LOCUS       DAOQLS010000001        13027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_1807_length_13027_cov_4.322541, whole genome shotgun sequence.
ACCESSION   DAOQLS010000001 DAOQLS010000000
VERSION     DAOQLS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1807_length_13027_cov_4.322541"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4371)
                     /locus_tag="PK362_00005"
     CDS             complement(<1..4371)
                     /locus_tag="PK362_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNC73256.1"
                     /translation="MNRFWRSGAAVALAVLAAAGCDRLRRPPAVKPPDPLWAQFLAGH
                     TVGIVSRKTKIAVRFAVDVFDKERVGQEAPGVLEFDPSVRGTATVSDPREVVFIPAED
                     LTPGAYYKITVRRKALANLPEKLDDYEFVFQTLPRTFEVEIGGLTAEDTAGLMALAGV
                     VTTADGEEAEPVEKVVFPQWSGAPLQVTWTHDAEGRRHEFTVRGLKREADAQSLHVNW
                     YGGPLDVENRGERTVEIPGLNDFKITNFQVVQAEERYFAVYFSDKIDPRQNLKGLIRA
                     SVSPFTLRVEGNVVKIYPDRRTSGKVTVTVEAGVRNAGGFALKEGRAETLSFVSEKPQ
                     VRFAGQGVLLPDNDVLSIPFDVMNVHSVQVTAFQVYEDNIGQFLQGNRLDTDTELGRV
                     GRHLWRKTLHLSAANPDQWQRYELDATDLLKKNPGALFRLTLSINRSNSDFACSEQDS
                     AVPVKTESAPKNQDDIYWNEGSGWDGIENWYQEGNPQWTDREDPCKDAYFIFGNGVKD
                     ARNFLASNIGLLAKTDATGRVTLVATDLRTAAPLSGAALSIRNFQNQEIGSGRTDGDG
                     FASIAVTEKPFYVAAEKDGGKGFLKMSDGTALPTSHFDVGGEKVQEGLKGFIYGERGV
                     WRPGDDLHLVFVLENKNHVVPEDHPATLRLTNPQGQLVDTVTNAKPVNGFYRFDLKTA
                     ESAPTGTWTAEVQLGGSAFSKALKIETVVPNRLKIEYAVGEEPIPAGNLNGTIFSQWL
                     HGATAAGLKTQVSVRYKSQGTRFARFEDHLFDDPTREIKGNEETIFEGRLDPTGHLAF
                     NSDLATDAPAPGMVSAQFKIQVFEEGGAFSVSQKAFDFAPYRRFVGLKLPKGDQVRNM
                     LLTDKTHTVSLVTVNAKGEPIKVPKLKVALYKVQWRWWWDQTGESSAQFNTGEENHVI
                     KEGTASTKADGRGSWDFEVKYPDWGRYLVRACDEDGGHCAGQYLYIDWPGWAGRAQEQ
                     SGPGASVLYFFSDKSSYAVGETAVLQLPDASQGRGLMTVENGSRVLEKRWIDFTKGKT
                     KFELPITAEMAPNVYVSVALLQPHAGKDNDRPLRLYGVIPLRVTDPDTVLQPVVKAPE
                     EWRPLSEAAVTVSETKGRPMTYTLAIVDEGLLGLTGYSTPDPHDYFYRKEALGVKTWD
                     LFDLVVGAYSANLERLLALGGSDALEGKDDNQETRRFPPVVRFLGPFTLGKGESKTHK
                     VDLPQYIGQVRAMVVAGRDGAYGRAEKSVYVREPLSVLTTLPRVVGPEERIAVPVNVF
                     VMKPEVKDVSLSIDTDDRFEVVGSKTAQMVFAQPGDGLTLFTLRAGLRTGPGRVTVSA
                     AGGGSSTRSETHLTVRAANPPTVETRGGAVEGGQTWTETVVPHGLPGTNEVHLEVSAL
                     PPLNLERRLGELIRYPHGCVEQVTSSVFPQLYLPDLVKLTDAQKAEVEKNIKTGIDRL
                     RAFQAPNGGFVYWPGALSGGP"
     gene            4525..5178
                     /locus_tag="PK362_00010"
     CDS             4525..5178
                     /locus_tag="PK362_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC73257.1"
                     /translation="MGNPLLRPFAPEDRTALRRLAVSTADRGNPSPGLAGDPALLADL
                     LTDYYAEKEPESLFIAEIDGAVRGYLTGCLDTRRYGRDMACRVVPRAVGRAALRGFFF
                     RPAAWGLFARLIRPGTPGNYRTPAILDRWPSHLHINLDPSARGRGVGKALIERFLHHA
                     RQKGSPGAHLAARIDNPGALRFFSSAGFREEGRRPLPGLLPGEPPQHDVALLVREIP"
     gene            5346..6662
                     /locus_tag="PK362_00015"
     CDS             5346..6662
                     /locus_tag="PK362_00015"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC73258.1"
                     /translation="MAVATTTVVLRDDDRLLVLAPHPDDDILCCGGLIQEAVDRGLPL
                     RVVYFTYGDNYEWAFMAYKKHPVIRRKAMQNMGLLRHDEAVAGMGVLGVSSGSLSFLG
                     YPDYGTLKIWNSRWDGRPPYRSMLTRVSKVPYVNAYRPGAAYKGEEIVKDLTSLLREF
                     RPTKIFVSHPGDQHPDHRALPLFLHVALWDLGPDFAPAVYPYLVHLRRWPSPPGHRPD
                     QPLAPPAAFRALAQWETFPVDAGRREAKRRALERHRTQFAYDRNYLLSFIRTNELFGD
                     FPILDLDRKGLPPPEDSDQERAKFVGVETKTVSVQNGKLVVTVSYQRPLGNRTGVQVF
                     AFGYRPGVPFERMPKLRIDFGALRHRVFDQGRELPWKSIEVDREPRRITLWIPLELMG
                     EPDRILLSSRSLFVSLPLDWATWHLVELGRRTSDPSERKESDGQNR"
     gene            6701..6922
                     /locus_tag="PK362_00020"
     CDS             6701..6922
                     /locus_tag="PK362_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73259.1"
                     /translation="MKKTLLVSGLFVGALSGLSLLAEGTTMTPVAEPTAAAAPAKAPA
                     KTHKKHKMMKKNAAAKPAETAPAAAPASK"
     gene            7048..7668
                     /locus_tag="PK362_00025"
     CDS             7048..7668
                     /locus_tag="PK362_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC73260.1"
                     /translation="MASHALSQRRIGTMIPDASTATPAAGTALDADRFRDLMERHGDK
                     IYNFAWRLAGNESDARDLTQEAFARAFQHRDRFDFTRPFDAWVCRILHNHFLDNVRRY
                     EHRRSISYDAPTEGLPLTETLVGRDPNPLDALMREERDRSVQRALNLLPPLYRSAVIL
                     CDLENYSYETIAEILECPLGTVRSRLHEGRRLLRQFFAAEAAGGSR"
     gene            7686..8141
                     /locus_tag="PK362_00030"
     CDS             7686..8141
                     /locus_tag="PK362_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNC73261.1"
                     /translation="MLSALIDGVLEVEEIALLEEHLALCADCRGELADLKRIQAALAA
                     APRRVLPAAFKAEMAQKILRPGWRERWAALWFRGPRWIPLGAAAAAALAVGLAWWGLG
                     GGRAEEETLRLEPFLAAHARTASDGPVPTSNFYGSDFSAQLASYNEAND"
     gene            8125..9114
                     /locus_tag="PK362_00035"
     CDS             8125..9114
                     /locus_tag="PK362_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HNC73262.1"
                     /translation="MKRTIKPLLGAAALLSAAVLRSGAAPSSDELMRRALKDESRTYA
                     GELAYTQWGDGRVRAEEARIYVAAPGIYRFEYLSPAGAVQRVILGRGADEIVFFPRQG
                     KAWRGPARRPPAADKSDRAFARLSKNYRVSTPVDDDIAGRPAWAITLTPVDAGKPHQT
                     LWFDQATGVLLASRRFLPGTPYAASVRFVRFDPDVDFEDGFFEMESGAVKRTDDHGWA
                     GRPGPSDPPPPAKAELPFGFALENRGQFEVQGETVTHLSYSDGLAVVSVFETRHPVRV
                     PSGAETPPPNAGPRLLNVSIPARVVHGRRDGVYLTVIGDDTAELLNELWRRFP"
     gene            9169..10101
                     /locus_tag="PK362_00040"
     CDS             9169..10101
                     /locus_tag="PK362_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73263.1"
                     /translation="MIDVQNLVKDYGPTRAVAGVSFRVEPGEVVGLLGPNGAGKSTTL
                     RILTGYLPPTAGRVVLDGVDVLENPLEARRRIGYLSEINPLYESLGVWECLELFGRLR
                     GMAPALLAKRRGPVVEECALTDVMHKDVGELSKGYRQRLGLALAILHDPQILVLDEPT
                     SALDPNQQKDVRDLILRLKEKKTVLLSTHILPEAQRMCDRLLIIHQGRIAAQGTVSDL
                     QARRTGAETVYVRLRGPAGEVGAALEKLSGVTAVQSVDEAEPGATGYHVTAAEDPRET
                     LVRLAVDRRWPLLELRRQSESLDDIFERLTGESA"
     gene            10098..10814
                     /locus_tag="PK362_00045"
     CDS             10098..10814
                     /locus_tag="PK362_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC73264.1"
                     /translation="MNLGRAGLLARKEWKESFNSPTPYIALGLFFILIGWFWTSALFL
                     TGQATMEEFFGPLPLLLGIFLPAFTMRLFAEEYKTGTIENLATLPLEDIDIVLGKFAA
                     ALAVWGVMLGLSLVYPALLLCLGSPDGGQAMAGYLGAFLLGVFFSALGLLASALTRSQ
                     VVAFLLGFLFCFLFYLVGKAAQFVPGVPGVLLAFLGVDRHMEGFLKGVVDSRDVLYFL
                     SGTALALAGTLAGYNSRRWR"
     gene            10811..12415
                     /locus_tag="PK362_00050"
     CDS             10811..12415
                     /locus_tag="PK362_00050"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNC73265.1"
                     /translation="MTTPAAERRRRMGLLSGTGLAVALGLLTFVNLLADRFFVRFDWT
                     NHRRYSLSPATKSLLRSLEDPVILRLYLSPGLPQPYASQGRYIRELLHEYRTAGHGKV
                     TLDIKSPDESDAWVMETRRLNIQPGRFTQVASDQFQVREGYMGLALFYQDKQDVIPFI
                     KETDNLEYEISSRVRLMTQKSKKTLFFVSNHQEVSPDFIRQGPAGRLFDEFRVETPSL
                     STATVGRRPDGLFLLGPQRSLSPEELDALDQALVDGIPVAVALNRRVIMPQNFRSMAQ
                     VTGLEPWLEHYGVNVSREFLMDEQCQNIAMRSAETTFYVKYWPFVLSNDLNREHLATR
                     DLDVLGFPYANPLSFNFSGASTLKGTVLARSSAKSWVWPGLYNVDPPSLFAQLKSESP
                     ATFGKSGKGDQLGPFPLAVVVEGATTTYRTPSRPIPNLRLVVLGTSFFANPQVPNPEG
                     NALSILALAHWMTQESNYLAIPPKDSPFRPLRPVSNGLRRTAKAAGYFLIPALIVLAG
                     FLHWRRRQILRARVQRAHARTEAPRA"
     gene            12408..>13027
                     /locus_tag="PK362_00055"
     CDS             12408..>13027
                     /locus_tag="PK362_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNC73266.1"
                     /translation="MPKWLRGSAILLGVLALIYAVAQIPRPEEKSPLPVVPERLEKLA
                     IARPPFSATFEYTNDHWRLRQPLDFPADDGAVASLLAGLRGMTGGDVLTRRAESHGLY
                     DVTDAAGVRVSLWAPGAKEPLEWILGKTSADGNHAYLRLPKSDAVFLVGGWTREGIEP
                     NLNAWREKRILTASSEDIVEVKAEPRGGAFTLSKDSDGWTVNGRPA"
BASE COUNT         2208 a         4346 c         3993 g         2480 t
ORIGIN      
        1 ggggcctccc gacagcgccc ccggccaata gacgaatccg ccgttgggcg cttggaaggc
       61 ccgcaggcgg tcgatgccgg ttttgatgtt tttttccact tcggcttttt gggcgtcggt
      121 gagcttcacc aggtccggaa gataaagctg gggaaagacc gaggaggtga cctgctccac
      181 gcacccgtgg gggtagcgga tcaattcgcc caggcgccgc tcgagattca acggcggtag
      241 ggccgacacc tccaggtgga cctcgttggt ccccggcagc ccgtggggga ccacggtttc
      301 cgtccaggtc tggccgcctt ccacggcgcc gccccgggtt tccacggtgg gcgggttggc
      361 cgcccgcacc gtgagatggg tttccgatcg ggtcgacgat ccgccgcccg cggccgacac
      421 cgtcacccgg cccgggcccg tgcgaaggcc ggctcgaagg gtaaagagcg tcaatccgtc
      481 gccgggctgg gcgaaaacca tctgggccgt cttggacccc accacctcaa agcggtcgtc
      541 ggtgtcgatg gacaaggaca cgtccttcac ttcgggcttc atcacgaaca cgttcacggg
      601 caccgcgatc cgctcctcgg ggcccaccac ccggggaagg gtggtcagga ccgacaaggg
      661 ctcccgcacg tagacggatt tttccgcccg gccgtaggcc ccgtcccggc ccgccaccac
      721 catggcgcgc acttgtccga tgtactgggg caggtccact ttgtgggttt tggattcgcc
      781 tttgcccagg gtgaaggggc ccaggaaacg aaccaccggc gggaaacgcc gggtttcttg
      841 attgtcgtcc ttgccttcga gggcgtcgct tccgcccagg gccaggaggc gttccaggtt
      901 ggcgctgtag gcgccgacca ccaggtcaaa gaggtcccag gttttgacgc ccagggcttc
      961 cttgcggtag aaataatcgt gcggatcggg cgtggaatac cccgtgaggc ccaggagccc
     1021 ctcatcgacg atggccaggg tgtaggtcat gggccggccc ttggtttccg aaacggtgac
     1081 ggcggcttcg gagagcgggc gccactcctc gggcgccttg accacgggct ggagaacggt
     1141 gtccgggtcc gtcacgcgga gggggatcac cccgtagagc cgaaggggcc gatcgttgtc
     1201 tttcccggcg tggggctgaa ggagcgccac cgaaacgtac acgttcggcg ccatttcggc
     1261 cgtgatgggc agctcgaatt tggttttgcc tttggtgaaa tcgatccagc gtttttcgag
     1321 cacccggctt ccgttttcga cggtcatcag gccccgtccc tgggaggcgt ccggcaattg
     1381 gagaaccgcg gtttcgccca cggcgtaact ggatttgtcc gaaaagaaat acaacacgct
     1441 ggcgccggga cccgattgtt cctgggcgcg gccggcccaa ccgggccaat cgatgtaaag
     1501 gtattggccc gcgcaatgcc cgccgtcttc gtcgcaggcg cgaaccaagt aacggcccca
     1561 atccgggtat ttgacttcaa aatcccagga cccccgcccg tccgccttgg tggacgcggt
     1621 gccttccttg atgacgtggt tttcctcgcc ggtgttgaac tgggccgagg attcgccggt
     1681 ttggtcccac caccagcgcc attggacctt gtagagggcg actttgagtt tcggcacctt
     1741 gatcggctcg cccttggcgt tgaccgtcac caaggacacc gtgtgggttt tgtccgtcaa
     1801 cagcatgttg cgcacctggt cgcccttggg cagtttcaac ccgacgaaac gccggtaagg
     1861 cgcgaagtca aaagccttct ggctgacgct aaaggccccg ccctcttcaa agacctgaat
     1921 cttaaattgc gccgagacca tgcccggggc cggggcgtcg gtggccagat cggaattaaa
     1981 ggcgaggtgc cccgtggggt ccaaacggcc ctcaaaaatc gtttcctcgt tgcctttgat
     2041 ttcccgggtc ggatcgtcga agagatggtc ttcgaaccgc gcgaatcgcg tcccctggga
     2101 tttgtaccgc acggacacct gggtcttcaa accggccgcc gtggctccgt ggagccattg
     2161 ggaaaaaatg gttccgttca aattccccgc ggggatcggc tcctccccga ccgcgtattc
     2221 gattttaagt cgattcggca cgacggtttc gattttaagc gctttggaga aagcgctgcc
     2281 gcccaattga acctcggcgg tccaggtgcc cgtgggggcg ctctcggccg ttttgagatc
     2341 gaaccggtaa aacccgttga ccggcttggc gttggtgacc gtgtcgacca gttggccctg
     2401 ggggttggtg aggcgcaggg tggccgggtg gtcctccggc accacgtggt ttttattctc
     2461 caggacgaag accaaatgga ggtcgtcgcc gggacgccaa acgccgcgtt ccccgtaaat
     2521 aaatcccttc agcccctcct ggactttttc gccgccgacg tcgaagtggc tggtgggcag
     2581 ggccgtaccg tcgctcattt tcaaaaagcc cttgccgccg tctttttcgg cggccacgta
     2641 gaagggtttt tccgtgacgg cgatgctggc gaacccgtcc ccgtcggtcc ggcccgatcc
     2701 gatttcctgg ttttggaaat tccggatgga cagggccgcg ccggacaacg gggcggccgt
     2761 tcgaagatcg gtcgccacga gcgtcacccg gccggtggcg tcggttttgg ccaacaagcc
     2821 gatgttcgac gccaaaaagt tccgggcgtc cttcacgccg ttgccgaaaa tgaaataggc
     2881 gtctttgcag gggtcctccc ggtcggtcca ctggggattg ccctcctggt accagttctc
     2941 aatgccgtcc cagccggacc cttcgttcca ataaatgtcg tcttggttct tgggcgccga
     3001 ttcggttttg accggcacgg cgctgtcctg ctcggagcag gcgaaatccg agttgcttcg
     3061 attgatggag agggtcagcc ggaagagggc gccggggttc tttttcaaca agtccgtggc
     3121 gtccagttcg tagcgttgcc attgatcggg gttcgccgcc gagaggtgga gggtttttcg
     3181 ccacaaatgc cggcccaccc ggcccaattc ggtgtcggtg tccaggcggt tgccctggag
     3241 aaactggccg atgttgtctt cgtacacctg gaaggccgtc acctgaaccg aatggacgtt
     3301 catgacgtcg aaggggatgg acaggacgtc gttgtcggga aggaggacgc cttgccccgc
     3361 aaagcgcacc tggggctttt cgctcacaaa agacagggtc tcggcccggc cttcttttaa
     3421 agcgaaaccg ccggcgttcc gcaccccggc ctcgacggtc acggtgacct tcccggaggt
     3481 ccgccggtcc ggataaattt tcaccacgtt gccttccacc cggagggtga aaggcgacac
     3541 gctggcgcgg atcaggccct tcaggttctg tcgcggatcg attttgtcgg aaaaatacac
     3601 cgcgaaatag cgctcctccg cctgaacgac ctggaagttc gtgattttaa aatcgttcaa
     3661 gccggggatt tcgacggtgc gctcgccgcg gttctcgacg tccaagggac cgccgtacca
     3721 attgacgtgg agggactggg cgtcggcctc ccgcttcagc ccccggaccg taaattcgtg
     3781 ccgccgccct tcggcgtcgt gggtccaggt cacctggagg ggcgccccgg accattgggg
     3841 gaacaccacc ttttccaccg gttccgcctc ttcgccgtcc gccgtggtca cgaccccggc
     3901 caaggccatg agacccgcgg tatcttcggc ggtcaatccg ccgatttcca cttcgaaggt
     3961 tcggggcaat gtttggaaaa cgaattcgta atcgtcgagt ttttcgggga ggttggccag
     4021 ggcctttcgg cggaccgtga ttttgtaata ggcgccgggg gtcaaatctt ccgcggggat
     4081 gaacacgact tcgcgggggt ccgacacggt ggccgtgccg cggaccgacg gatcgaattc
     4141 caaaacgccg ggggcttcct ggcccacgcg ttccttgtcg aaaacgtcca cggcgaaacg
     4201 cacggcgatc ttcgttttcc gggaaacgat gccgacggtg tggcccgcga ggaactgggc
     4261 ccacagggga tccggcggtt tcaccgcggg cggacgacgc agacggtcgc accccgccgc
     4321 cgccaagacc gccaacgcca ccgccgctcc gctccgccaa aatcgattca ttgaacgctc
     4381 ctcaaaaggg ttccccgatt tccggagggg ccccggtcca gggcacggga actagtatag
     4441 attgtcgtcg ctccggtcgc caccgaaaaa ccgcccggtg tccccggacc ggcccccgcc
     4501 ggggaagtga taaaatggcg gcccatgggg aatcccctcc tgcggccttt cgcgcccgaa
     4561 gatcggacgg ccctgcgtcg cctggccgtt tcaaccgccg atcggggaaa tccgtccccc
     4621 gggttggccg gcgacccggc cttgctcgcc gatctgctca cggattatta cgcggagaag
     4681 gaacccgaat cccttttcat cgccgaaatc gacggggcgg tccggggtta tttgacgggc
     4741 tgcctggaca cccggcgcta cggacgggac atggcgtgcc gggtggttcc ccgggccgtc
     4801 gggcgggcgg ctcttcgggg atttttcttt cgacccgccg cctggggcct gttcgctcga
     4861 ttgatccgcc ccgggacacc gggaaactat cggacaccgg cgattttaga ccgatggccg
     4921 tcccatctcc acatcaatct ggacccatcg gcccgcggcc ggggcgtggg aaaagctctg
     4981 atcgaacgat tcctccatca cgcccggcaa aagggttcgc cgggggcgca tttggcggcg
     5041 cgcatcgaca accccggggc ccttcgattt ttttcctccg cgggatttcg ggaggagggg
     5101 cgccgaccgc tcccgggcct gttgcccggc gaaccgcccc agcacgacgt cgcattgttg
     5161 gttcgggaaa ttccatgaaa aaaataatct tcggggcgat aatgcttttg gcggcgccga
     5221 tcctcggtga gggagggccc gtcgaggccg tcgccgacgg tctttccacc cccccgatcg
     5281 tcgcgtcgcc cgcgcccaga aaaaaggtcc gccgcccgcg gcgcccccgt cgggcggccg
     5341 tccccgtggc cgttgccacc accaccgtcg ttcttcggga cgacgatcga ctgttggtcc
     5401 tggcccccca ccccgacgac gacattcttt gctgcggcgg tttgattcag gaggcggtgg
     5461 accgcggact cccgctccgc gtcgtgtact tcacctacgg cgacaattac gaatgggcct
     5521 tcatggcgta caaaaagcac ccggttattc gacgaaaggc catgcagaac atgggccttc
     5581 tccgccacga cgaggccgtg gcgggcatgg gggttttggg ggtgagcagc gggtccctct
     5641 ctttcctggg ataccccgac tacggcacct tgaaaatctg gaactcccgc tgggacggcc
     5701 gtccgccgta ccggagcatg ctcacccggg tgtccaaggt gccctacgtc aacgcctacc
     5761 ggcccggggc cgcctacaag ggcgaggaaa tcgtgaagga tttgacatcc ctcctccggg
     5821 aatttcgccc gacaaaaatt ttcgtctcgc accccggcga tcaacacccc gaccaccggg
     5881 ccctgccgct tttcctccac gtggccctct gggacctggg ccccgacttt gccccggccg
     5941 tttatccgta tttggtccat ctgcgccgtt ggccgtcgcc gccgggccat cgcccggatc
     6001 aacccctcgc gcccccggcg gcctttcgcg cgctggccca atgggaaacg tttccggtgg
     6061 acgccgggcg gcgggaggcc aagcgccgcg ccctggaacg gcaccggacc caattcgcct
     6121 acgatcgaaa ttacctcctt tcgtttattc gaaccaacga actcttcggc gatttcccga
     6181 tattggattt ggacagaaaa ggattgcccc cgccggagga ttccgatcaa gagcgggcca
     6241 aattcgtggg ggttgaaaca aaaaccgtca gcgttcaaaa cgggaaattg gtggtgaccg
     6301 tctcctacca gcgacccctg ggcaaccgca ccggggtgca ggttttcgcc ttcggctatc
     6361 gcccgggcgt tccctttgag cgaatgccca agctgagaat tgatttcggc gcccttcggc
     6421 accgggtttt cgaccaggga cgggaattgc cctggaaatc cattgaagtg gaccgggagc
     6481 cccgccgaat caccctttgg attccgttgg aattaatggg agagccggat agaatcctat
     6541 tgagttctcg atcccttttc gtgtccctcc ccctggattg ggccacttgg cacctcgtgg
     6601 agctgggccg gcgaacttcc gatccatccg aacgtaagga aagcgacggc caaaaccgtt
     6661 aaagaggagc ggggagtccc cgcccaccaa ggagaatcac atgaaaaaga cgctgcttgt
     6721 ttccggcttg ttcgttggcg cgctttccgg gttgtcgctt ctggccgaag gaacgaccat
     6781 gaccccggtt gcggaaccga cggccgccgc cgcgcccgcc aaggccccgg ccaagaccca
     6841 caaaaagcac aaaatgatga agaagaacgc cgccgcaaaa cccgcggaaa ccgcgcccgc
     6901 cgcggccccg gcgtcgaaat aaattgaacg acggccgatc gtgactcgat cggcgtttcg
     6961 ggccccgacc tccacgggaa cgacccgggc gacccccgct ccgggtcgtt ccccggaggg
     7021 ggtctgtccg ggaaaggttc aaaaaccatg gcctctcacg ctctctccca acggcggatc
     7081 ggaaccatga tccccgacgc ctcaacggcg acccccgccg ccggaaccgc cctcgacgcg
     7141 gatcgatttc gtgatctgat ggagcgccac ggcgacaaga tttacaattt tgcctggcga
     7201 ctcgccggca acgagtccga cgcccgggac ctcacccagg aagcctttgc ccgggccttc
     7261 caacaccggg accggttcga tttcacccgt cccttcgacg cctgggtgtg ccgaattctc
     7321 cacaaccatt ttctggacaa cgtccgtcgc tacgagcacc ggcgttcgat ctcttacgac
     7381 gcgccgacgg aaggccttcc cctcacggag accctggtcg gccgggatcc caaccccctc
     7441 gacgccctga tgcgggaaga gcgggaccga tccgttcaac gggcgctgaa ccttttgccg
     7501 cccctttacc gttccgccgt gatcctttgc gatttggaaa attactccta cgagaccatc
     7561 gcggaaatcc tggaatgccc cctgggcacg gtgcgttccc gccttcacga gggacggcgc
     7621 ctgctgcggc aattcttcgc cgccgaagcg gccggaggct cccggtgagc gcgcatcccc
     7681 attccttgct gtccgccctg atcgacgggg ttttggaggt cgaggaaatc gccctgctgg
     7741 aggagcacct ggccctctgc gcggactgcc gcggggaatt ggcggatttg aagcggattc
     7801 aggcggccct ggccgcggcc ccccggcgcg ttctgcccgc cgccttcaag gccgaaatgg
     7861 cccaaaaaat ccttcggccc ggatggcgcg agcgatgggc cgcgttgtgg ttccggggcc
     7921 cccgctggat tcccctgggc gccgccgcgg ccgccgcctt ggccgtcggc ttggcctggt
     7981 ggggcttggg gggcggacgg gccgaagaag aaacgctccg cttggaaccg ttcctcgccg
     8041 cccacgcccg caccgcgtcg gacggtccgg tcccgacctc gaatttctac ggctcggatt
     8101 tttccgccca gttggcctcc tacaatgaag cgaacgatta aaccccttct cggagcggcc
     8161 gcgctcctgt ccgccgccgt tctccgaagc ggggcggccc cctcgtccga cgaattgatg
     8221 cgccgggccc tgaaagacga atcccggacc tacgccggcg aattggccta cacccaatgg
     8281 ggcgacggcc gggtccgggc cgaagaggcc cgcatttacg tggccgcccc ggggatttac
     8341 cgtttcgaat acctctcccc cgccggcgcc gttcagcggg tgatcctggg ccggggcgcc
     8401 gacgagatcg tgtttttccc ccgccaggga aaagcctggc ggggacccgc ccgccgtccg
     8461 cccgccgccg acaaatcgga ccgggccttc gcgcgcctat ccaaaaatta ccgggtgtcg
     8521 acgccggtgg acgacgacat cgccggccgc cccgcctggg ccatcacctt aacccccgtg
     8581 gacgccggca aaccccatca aacgctgtgg ttcgaccaag ccaccggcgt cctcctggcc
     8641 agccgccgct ttttgccggg gaccccttac gccgcgtcgg tgcgcttcgt ccgcttcgac
     8701 cccgacgtgg attttgaaga cgggtttttt gaaatggaat ccggcgcggt gaagcgcacc
     8761 gacgaccacg gctgggcggg gcgccccggg ccctccgatc ccccgccccc ggccaaggcc
     8821 gaacttccct tcgggttcgc cctggaaaac cgcggccagt tcgaggtcca gggggaaacc
     8881 gtcacccatc tgtcctattc cgacggactc gccgtggtgt cggtgttcga aacgcgccac
     8941 ccggtgcgcg tgccatcggg cgcggaaacg ccccccccga acgcggggcc ccggttgttg
     9001 aacgtttcga tccccgcccg ggtcgtccac ggccgccggg acggcgtgta tctgacggtg
     9061 atcggcgacg acaccgccga gcttttaaac gagctttggc ggcgtttccc ttaattgact
     9121 cgtcgggtaa ttttgtagaa tccgtgatcg gcacctaaat ccgaccccat gatcgacgtt
     9181 caaaacctcg ttaaagatta cggccccacc cgcgcggtgg cgggcgtttc ctttcgcgtg
     9241 gaacccgggg aagtggtggg cctgctgggc cccaacgggg ccgggaaaag cacgacgttg
     9301 cggattttaa cgggttacct tccccccacc gccggccgcg tcgtcctgga cggcgtcgac
     9361 gtcctggaaa accccctcga agcccgccgg cgcatcgggt atctttccga aatcaatccc
     9421 ctttacgaat ccctgggcgt ttgggaatgc ctcgaattgt tcggccgcct gcggggcatg
     9481 gcccccgccc tcctcgccaa acggcgcgga cccgtggtgg aggaatgcgc cttgaccgac
     9541 gtcatgcaca aggacgtggg ggagctgtcg aaaggttatc gccagcgcct gggattggcc
     9601 ctcgccatcc ttcacgaccc gcaaatcctc gtcctggacg agccgacctc ggccctggac
     9661 cccaaccagc agaaagacgt gcgggatctc atccttcgcc tcaaagagaa aaaaaccgtc
     9721 ctcctctcca cccacatttt gccggaagcc caacgcatgt gcgaccgcct gttgattatt
     9781 caccaggggc ggatcgccgc ccagggcacg gtgtcggacc ttcaggcgcg ccggaccggc
     9841 gccgaaaccg tttatgtccg cctgcgaggt cccgccggcg aggtcggcgc cgccctggag
     9901 aaactatcgg gcgtcaccgc cgttcagtcc gtggacgagg cggagcccgg cgccacgggg
     9961 tatcacgtca ccgccgccga ggacccccgg gaaaccctgg tgcgcctggc ggtggatcgc
    10021 cgatggcctc tcctggaact gcgccgccaa tcggaatccc tggacgacat atttgagcgg
    10081 ttgacgggag aatccgcgtg aacctcggtc gcgccggcct tctggcccgg aaagaatgga
    10141 aggaatcctt caattccccc acgccttaca tcgccctggg cctgttcttc atcctgatcg
    10201 gatggttctg gacgtcggcc cttttcctga cgggacaggc gacgatggag gaattcttcg
    10261 gccccctgcc gctcctcctg ggcatctttc ttcccgcgtt caccatgcgg cttttcgccg
    10321 aggaatataa aaccgggacc attgaaaacc tcgccaccct gccgctggag gacatcgaca
    10381 tcgtcctggg caaattcgcc gcggccctgg ccgtgtgggg cgtgatgctg gggctctcgc
    10441 tcgtgtatcc ggccctcctg ctttgcctgg gctcgcccga cgggggccaa gcgatggccg
    10501 gctacctggg ggcttttctg ctcggcgtct ttttttcggc cctcgggttg ctggcctcgg
    10561 ccctgacccg gagccaggtc gtggccttcc ttctgggatt cctcttttgt ttcctgtttt
    10621 atctggtggg aaaggccgcc cagtttgtgc ccggggtccc gggggtgttg ctcgcctttt
    10681 tgggcgtgga ccgccacatg gaaggcttct tgaaaggcgt cgtggattcc cgggacgtcc
    10741 tgtatttcct gtcgggaacg gccctggccc tggccggcac cctggccggg tacaacagcc
    10801 ggagatggcg atgacgaccc ccgccgccga acgccgccgt cgaatgggcc ttttgtccgg
    10861 cacgggcctc gccgtggccc tgggcctgtt gaccttcgtc aatttgctgg ccgaccgatt
    10921 tttcgtgcgc ttcgactgga cgaaccaccg ccgttattcc ctgtcgccgg ccacgaaatc
    10981 gttgctgcgc tccctcgaag accccgtgat ccttcggctt tatctgtcgc ccggactgcc
    11041 ccaaccctac gcgagtcagg gacgctacat tcgggaattg ctccacgaat accggaccgc
    11101 cggccacggg aaagtcaccc tcgacatcaa atcgccggac gagtcggacg cctgggtcat
    11161 ggaaacccgc cggctcaaca ttcagccggg ccggttcacc caggtcgcct cggaccagtt
    11221 ccaggtccgc gaagggtaca tgggcctggc cctcttttac caggacaagc aggacgtcat
    11281 tcctttcatc aaagagaccg acaacttgga atacgagatt tccagccgcg tccgcctcat
    11341 gacgcagaaa tccaaaaaaa cgcttttctt cgtctccaac caccaggaag tttccccgga
    11401 tttcatccgc cagggaccgg cggggcgcct cttcgacgaa ttccgggtgg agaccccgtc
    11461 gttgagcacg gcgaccgtgg ggcgccgccc cgatggcctt tttctgctgg gcccccagcg
    11521 gtccctctcc ccggaggaac tcgacgccct ggaccaggcg ctggtggacg gaatccccgt
    11581 ggcggtggcc ttgaaccggc gggtcatcat gccccaaaat ttccggtcca tggcccaggt
    11641 cacgggcttg gaaccctggt tggaacatta cggcgtgaac gtcagccgcg aattcctcat
    11701 ggacgaacaa tgccagaaca tcgccatgcg gtcggccgaa accacctttt acgtcaaata
    11761 ctggcccttt gtcctctcca acgacttgaa tagggagcac ttggccaccc gggacctgga
    11821 cgtgctgggt ttcccctacg ccaacccgct gtcgttcaat ttcagcggag cgtcgacgct
    11881 caagggcacc gtgctcgccc gctcctccgc aaaaagctgg gtttggcccg gcctctacaa
    11941 cgtcgacccc cccagcctgt tcgctcaatt gaaatccgaa tcccccgcga ccttcggcaa
    12001 atccgggaaa ggggatcaac tggggccctt tcccctggcc gtggtggtgg aaggcgccac
    12061 caccacttac cggaccccgt cccggcccat cccgaacctc cgcctcgtgg tcctgggcac
    12121 gtcctttttc gccaatcccc aggtgccgaa ccccgaaggc aacgcgctct cgattctcgc
    12181 gctggcccat tggatgaccc aggaaagcaa ctatctcgcc attccgccga aagactcccc
    12241 cttccggccc ctgcgcccgg tgtccaacgg ccttcgacga acggccaagg cggccggtta
    12301 tttcctgatc ccggcattga tcgttttggc gggattcctt cactggcgtc gacgtcaaat
    12361 ccttcgggcg cgcgtccaga gggcccacgc ccgaacggag gccccccgtg cctaaatggt
    12421 tgcgcggctc ggcgatcctc ctgggcgttc tcgccttgat ttacgccgtc gcccagatcc
    12481 cccgaccgga ggagaagtct cccctgccgg tggttcccga acgcctggag aagcttgcca
    12541 ttgcccggcc gcccttttcc gccacgttcg aatacaccaa cgaccactgg cgcctgcgcc
    12601 aaccgctgga tttccccgcc gacgacgggg cggtggcctc ccttttggcg ggtctgcggg
    12661 gaatgacggg cggcgacgtc ctcacccggc gcgcggaaag ccacgggctc tacgacgtga
    12721 ccgacgcggc gggggtgcgg gtgagccttt gggcgcccgg ggccaaggaa cccctcgaat
    12781 ggattttggg caaaacaagc gccgacggga accacgccta tcttcgactt ccaaaaagcg
    12841 acgcggtttt tctcgtgggc ggatggaccc gggaaggcat cgaaccgaat ttgaacgcct
    12901 ggcgggagaa acgaatccta acggcttcct cggaggacat cgtcgaggtt aaagcggaac
    12961 cgcggggcgg ggctttcacg ctgtccaaag attccgacgg ctggacggtc aacggacgcc
    13021 cggcctt
//
LOCUS       DAOQLS010000002        11548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_2165_length_11548_cov_4.019838, whole genome shotgun sequence.
ACCESSION   DAOQLS010000002 DAOQLS010000000
VERSION     DAOQLS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2165_length_11548_cov_4.019838"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PK362_00060"
     CDS             complement(<1..335)
                     /locus_tag="PK362_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HNC73267.1"
                     /translation="MKVPASGPEVKALAADLGFPFAAFTAIHPAAEDAAFLERWVAEG
                     KAAGMGWLSREPARRGNPANLLAGARTLISLGVPYAGETLPPRPAEPVGRVARYAWGL
                     DYHGTIQDRL"
     gene            complement(332..1081)
                     /locus_tag="PK362_00065"
     CDS             complement(332..1081)
                     /locus_tag="PK362_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNC73268.1"
                     /translation="MKKERMWIVGHRGAMGHAPENTRASFEKALRLGADAVECDVHRS
                     KDGETVVLHDGRLDRTTNGRGPVRSRTWAQLRKLDAGAWFAPRFKKERLWRLGDLIRW
                     ARRKKTRAGRPLNLIVEIKTSPDSPRRLPAAVVEALAAGGMVDRTIVISFDPRAVRAV
                     KARCPALRAGLLFSNPLPGLTRRMAALGADAIFPKHTLIDRALMTTARRHGWFVGTWT
                     ANETAELRRLSSLGVHAAATNYPERAPRPRS"
     gene            complement(1078..1494)
                     /locus_tag="PK362_00070"
     CDS             complement(1078..1494)
                     /locus_tag="PK362_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNC73269.1"
                     /translation="MTAPLLPTINAGFNALSAALLTAGFLFIRKKNVAAHRRCMTGAF
                     LSSVFFLAGYLLHHARAGHVRFQGAGLWRPLYLLLLLSHTVLAAAVVPLVVTTLALAA
                     RERFDRHRVWARWTWPVWMYVSVTGVAIYFALYHFS"
     gene            complement(1491..2273)
                     /locus_tag="PK362_00075"
     CDS             complement(1491..2273)
                     /locus_tag="PK362_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC73270.1"
                     /translation="MSASRFWLATGTLVDREMVRFFRQRNRLIGALMTPLMFWFFLGA
                     GLGGSFRVSLGAAGGVSYMQYFLPGTMALVCLFTAIFSTMSVIDDRQAGFLQAVLVSP
                     APRGAVVAGKMLGGTLLAFFQAVLFMALLPVLRVPLGLAGFLISSAMLLLMSFGLTGL
                     GLIIALKTDSVQGFHAIMNLFLMPLWFLSGAVFPASGAPGWLKVLMAINPMSYSVAAL
                     HRGFFPGAGGASLWVCFGVNALFAALTFGLSLSLIRRGGEAA"
     gene            complement(2270..3220)
                     /locus_tag="PK362_00080"
     CDS             complement(2270..3220)
                     /locus_tag="PK362_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73271.1"
                     /translation="MNPAVRVDGLRHEYKNPGGPPRVALQGIDFEVSPGELFGVLGPN
                     GGGKTTLFRILSTALRPTGGGARLFDTDVTSAPDAARQKMGVVFQSPSLDKKLTVMEN
                     LRHQGRLYGLHGAELAARSAEMLAHLGLADRAGDIVERLSGGLQRRAEIAKSLLHRPA
                     LLLMDEPTTGLDPGARRDVWTYLKSLKKEGVTVLVTTHLMEEAERCDRLIILDRGRIA
                     ALGTPAVLKETIRGDVVWVTTVSPDILSAGLKERFDVDAVVQDGAVRFERDRAHELIP
                     QIVVAFPDLVASASVGKPTLEDVFVHHTGHRFWADNGGPS"
     gene            complement(3217..4959)
                     /gene="cyoE"
                     /locus_tag="PK362_00085"
     CDS             complement(3217..4959)
                     /gene="cyoE"
                     /locus_tag="PK362_00085"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNC73272.1"
                     /translation="MSETSAVPSPWPHRLAWVLAAATLALVFIGGLVTSTHSGLTVPD
                     WPLSYGRLLPPMIGGILFEHGHRLAAASVGLLTIVLNLVFWKTESRAWVRRAAIGALG
                     LVVLQGVFGGLTVLLRLPKAVSITHACLAQTFFCLSVALVVWTSRAWKERFPSAGGDP
                     VNVALPHVALLLFLTLYAQLVLGAVVRHTGHAAGLHIANAVLIFLGLGWVFRRLALGD
                     ETDPRLWRLASALALVYVVQIVLGTLTLLSLLVPQWISSPLRPVLLATGHVMTGALLL
                     GLSAALTLLSWRGRPVAFGRFRDYVTLTKPGISLMAAVTALAGVFLGSGGAVGPARLL
                     HTVVGTLLVSGGACALNMLLERDVDARMHRTENRPLPARRLNAGEALFLGSLLALAGV
                     LYLSAAVNVLTAVIAGLTLCVYLYVYTPLKKISALNTLFGAVAGALPPVIGWTAAVGR
                     LDPGAWVLFAILFFWQYPHFFSLAWLYRADYARAGLAMLPAVEPDGETTARHMVVTTL
                     ALTSVSLLPTFLGLAGTIYFAAAFLMGLGLLALSFVFFRQHTDLRARRVFLASVLYLP
                     LLLLILVLDRRGTL"
     gene            complement(4989..5210)
                     /locus_tag="PK362_00090"
     CDS             complement(4989..5210)
                     /locus_tag="PK362_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73273.1"
                     /translation="MRKLPAAALVSMIAVNARACAVCFGAPGSALNRGFFWAILLLLV
                     LPFLLMGSLVALVVYHIRKGQRPTHPLPQ"
     gene            complement(5207..5491)
                     /locus_tag="PK362_00095"
     CDS             complement(5207..5491)
                     /locus_tag="PK362_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNC73274.1"
                     /translation="MDNQKSPVPLYAAVFGGLALLTGVTVLLSYLHLSHRVAIPLAAL
                     IALTKCTLIASVFMHLKWEGKAIRALLFTGLFLVAVLVLAVLPDIGVARP"
     gene            complement(5494..6081)
                     /locus_tag="PK362_00100"
     CDS             complement(5494..6081)
                     /locus_tag="PK362_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNC73275.1"
                     /translation="MSESAAPSFPPSTPIPSGKLGMWVFLSSEIMLFGAFISAFIVLR
                     MGSPNFGVPSEEVMGRPLATVNTFLLILSSFTMVLALAAIQRDDRRSFARNMIATMGL
                     GILFLIVKMFEYHHKISEGLTISSGLFGSFYFTLTGLHALHIIGGLVFNGYVLRNGLR
                     GRYSARHHERVEYAGLYWHFVDLVWVILFPMFYLV"
     gene            complement(6081..7796)
                     /locus_tag="PK362_00105"
     CDS             complement(6081..7796)
                     /locus_tag="PK362_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC73276.1"
                     /translation="MHEEPRQKSFWRTYVFSLDHKVIGLQYLFTSMAFLLLGFAFILL
                     MRWQLAFPGRPLPLIGGLLPDAIAPGGVMLPALYNQLGAMHGTIMIFLGVVPLGVGFF
                     GNYFLPLQIGAPDMAFPRLNMSSYWVYAMGGVVMLLSFVLPGGPAQSGWTSYPPLSDI
                     NPGQTIWLIGMTFLITSSLLGSINFIVTTVNLRAPGLTWSRLPLFVWAQLVTAILLLF
                     AFPVLQSGAILQLLDRVAGTCFYLPMGLVVGGKTLDRVGGGSPLLWQHLFWFLAHPEV
                     YVLVLPAMGIVSEIIAANTRKPLFGYTTMVGSMIFVGVFSFLVWAHHMFVSGMKTSLS
                     MFFMTTTMVISIPSVAILSCLLFSLWGGKIRFTVPMLFALAFLPMFGIGGLTGLPLGF
                     LPTDIYLHDTYYVIGHFHYVVVTGTIIALMAGVYHWFPKMFGRSMDDRLGQIHFWGTL
                     IGMNGIFLPMFIQGLAGMQRRLYDPTVQTHNLTTQFLSPYQLASAILLLLFQIPFLYN
                     FFKSMTKGVVARENPWDATTLEWACPSPPPHGNFPSVPSAHWAPYEYSVPGRAKDFWP
                     QWEKN"
     gene            complement(7805..8605)
                     /locus_tag="PK362_00110"
     CDS             complement(7805..8605)
                     /locus_tag="PK362_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73277.1"
                     /translation="MDELLGNYRVGLPVAASTYAGRVDTSLTVLHVGMIAIFLLWLAF
                     FVYCLWRFQQKKVDRASYQGWKGHASSFVPDALVLVFELWLIFVFGLPIWSHIKQDFP
                     AEDASNVVGLSAEQFSWGFHYPGPDGKLGRRDPARVTVSNPLGLDDTDPAAADDLVLY
                     NELHVPLGKPTILQMTSKDVIHSFFVPEFRVKQDVVPGMKIPLWFEPNRVGRFEIGCA
                     QLCGAGHYVMRGEIVVQTPEEYAQWWAGVSQAKAAAKPKVVAASDWSE"
     gene            complement(8626..9315)
                     /locus_tag="PK362_00115"
     CDS             complement(8626..9315)
                     /locus_tag="PK362_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC73278.1"
                     /translation="MSRFKVLLLLVLLAGCSGSKDPVARGRSYFVGLGCITCHRVGDK
                     GGGQAGPDLTTVGFRKTPEWLRLWLANPAAWKPGTAMPNFKLKDNVREDLVAYLSSLK
                     GEIYRAKPPWDNPALKADPLKRGEMLFARVGCVGCHGERGQGGYPNNNVVGGKILSLT
                     YAADGYSKDELRDLLHKGRVSLPADASQPPPMIRMPPWGQYLTDDEINDLVEYVYSLR
                     PATAAKDDWSE"
     gene            9400..10116
                     /locus_tag="PK362_00120"
     CDS             9400..10116
                     /locus_tag="PK362_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73279.1"
                     /translation="MTPRDSSPLGFRRIRRFLAAPGVLFLALIGPAAVPAWADMDDNP
                     SYLKDWTERDKGSGWNFSPAFNGGYTSLLHGYKGYKNLGNAGLDLYMRPPVPQFPRWY
                     NHMIFRLSADYFPLQVPSQVVGITEDIYALTGTALWRFSNASEAEHNQWIPFLGAGVG
                     VYWDSVTVDTLATGKVKDAEQYLGVTGALGVMLPTWGPIRIIPEIRYHTLREAGRYWA
                     SHMTYQIGAALWFPAKVSEQ"
     gene            10113..11060
                     /locus_tag="PK362_00125"
     CDS             10113..11060
                     /locus_tag="PK362_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC73280.1"
                     /translation="MNRPLPRLAAVVLALLAVGGCKRKDNNPLVGTPGAGVTGVTLAV
                     RGVDNTQFPDIKVLLQPKTSGGAPITDLTVGNFQILQGGKAGPIPTGYESASGYPFSV
                     MLVIDHSGSMSADDGTGSSRLARAQTAAAAFLGALSSNDEAAFIEYDDSVNFVQGFTK
                     DKNTVIAAVNASTPAGATATFDAIVRGAEELSKGTGLRLLVFLTDGDDNSSSNTAATV
                     KTKLSQAGVVACGIVIAGGATDTATLDDIAQSTGGQLLAGTTTVELQTAFNTILSSGL
                     FDDIYGLSFRSKNGQGSATYSVYINYGAFTDRADLIDFL"
     gene            complement(11136..11435)
                     /locus_tag="PK362_00130"
     CDS             complement(11136..11435)
                     /locus_tag="PK362_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC73281.1"
                     /translation="MTDLPKINVDEKLGTLTYKADHHTHITIKSADVCAKKCDGKPCT
                     TVCPAQVYRWETDQKKIIVSFENCIECGACRMLCPFDNIDCHWPRGGFGVQYKYG"
BASE COUNT         2510 a         3484 c         3743 g         1811 t
ORIGIN      
        1 agacggtcct ggatggtgcc gtggtagtcg aggccccagg cgtagcgggc cacccggccg
       61 accggctcgg ccggtcgcgg gggaagcgtc tcgcccgcgt agggaacgcc caggctgatc
      121 aacgtgcggg ccccggccag gaggttggcg gggtttcccc gtcgggccgg ttcccgggac
      181 aaccaaccca tcccggcggc cttgccttcg gcgacccatc gttccaggaa agcggcgtcc
      241 tccgccgcgg ggtggatagc ggtgaaagcc gcgaaaggaa aaccgagatc cgccgcgagg
      301 gctttgacct ccgggccgga cgccgggacc ttcatgaacg cgggcggggc gcccgctcgg
      361 ggtagttggt cgccgccgcg tggacgccga gcgacgacag gcgccgaagc tccgccgttt
      421 cgttggccgt ccaggtcccc acaaaccatc cgtggcggcg ggccgtggtc atcagggccc
      481 ggtcgatcaa ggtgtgtttg ggaaatatgg cgtcggcccc gagggcggcc attcgccgag
      541 tcaggcccgg caggggattc gaaaaaagca atcccgcccg gagagccggg caccgggcct
      601 tgacggcccg gaccgcgcga gggtcgaagg agatgacgat cgttcgatcc accatcccgc
      661 ccgcggccag ggcttccacc acggcggccg ggaggcggcg gggactgtcc ggggaggttt
      721 tgatttcaac gatcaaattc aggggccgac ccgcgcgggt ttttttccgc cgggcccaac
      781 gaatcaaatc ccccaggcgc caaagccgtt cttttttgaa ccggggggcg aaccaggccc
      841 cggcgtcgag ttttcgaagt tgagcccagg tccgggaccg gacgggcccc cgcccgttgg
      901 tggttcggtc caggcggccg tcgtgaagga cgaccgtttc gccgtctttg gagcgatgca
      961 cgtcgcactc cacggcgtcg gcgcccaggc gcagggcttt ttcaaaggac gcgcgggtgt
     1021 tttcgggcgc gtggcccatg gccccccggt ggcccacaat ccacatccgt tcttttttca
     1081 cgagaaatgg tacagcgcga aataaatggc cacgcccgtc acggacacat acatccagac
     1141 gggccaggtc caacgggccc agacccggtg ccggtcgaac cgctcccgcg ccgccagggc
     1201 caaggtggtc acgaccaggg gcaccacggc cgcggccagg accgtgtggg agaggagaag
     1261 aagaaggtag aggggacgcc aaaggccggc gccttggaac cgaacgtggc ccgcccgggc
     1321 gtgatggagg agataaccag ccaaaaagaa gaccgaggac aagaaggccc cggtcatgca
     1381 acggcgatgg gcggcaacgt tcttttttcg aatgaaaagg aatccggcgg tgaggagggc
     1441 ggcgcttagc gcgttgaaac cggcgttgat cgttggaagg agaggcgcgg tcacgccgcc
     1501 tctccaccgc gccgaatcag cgacagggag agaccgaagg tcaaggcggc gaagagggcg
     1561 ttgacgccga agcacaccca gagggacgcc ccgcccgccc cggggaaaaa gccccggtgc
     1621 agggccgcca cgctgtagct catgggattg atcgccatga gaaccttgag ccatcccggc
     1681 gccccggagg cggggaacac cgcgccggac aggaaccaca ggggcatgag aaaaagattc
     1741 atgatggcgt ggaacccctg gacggagtcg gtcttcaagg cgatgatcag gccgagaccc
     1801 gtgaggccga aggacatgag gagcagcatg gccgaggaaa tcagaaaccc ggccaggccg
     1861 aggggcacgc gcaaaaccgg cagtagagcc ataaaaagca ccgcctggaa aaaagccagc
     1921 aaggtgccgc ccagcatttt cccggccacc acggcgcccc ggggcgccgg cgacacgagc
     1981 acggcctgaa ggaaaccggc ctggcggtcg tcgatgaccg acatggtgga aaaaatcgcc
     2041 gtgaagagac acacgagggc catggtgccc ggaaggaagt attgcatgta gctcacgccc
     2101 cccgccgcgc ccagggacac gcggaagctt ccgcccaacc ccgcccccaa aaagaaccaa
     2161 aacataagcg gcgtcatcag ggcgccgatc agccggttcc gttggcggaa aaagcggacc
     2221 atctcccggt ccaccagggt gcccgtggcc agccaaaacc gcgacgcgct cacgagggtc
     2281 ccccgttgtc ggcccaaaaa cgatggccgg tgtggtgaac gaaaacatct tccagggtgg
     2341 gtttgccgac ggaggcggag gccacgaggt cgggaaaggc gaccacgatt tgcggaatta
     2401 attcgtgggc ccggtcccgc tcaaagcgaa cggcgccgtc ctgaaccacc gcgtcgacgt
     2461 cgaaacgctc cttgagcccg gcggataaaa tatccggcga gacggtggtt acccagacca
     2521 cgtccccccg aatcgtctcc ttcaacaccg ccggagtccc gagggccgcg atccgccccc
     2581 ggtccaaaat aatcaggcgg tcgcaacgct cggcctcttc catgaggtgg gtggtgacca
     2641 gaaccgtcac cccctctttt ttcaagctct tcagataggt ccaaacgtcc cgccgggccc
     2701 ccggatccaa ccccgtggtg ggttcgtcca tcagaagcag ggcggggcgg tggagcaggc
     2761 tcttggcgat ctcggcccgc cgctgcaggc cgccggaaag ccgttccaca atgtcccccg
     2821 cccggtccgc caggcccaaa tgggcgagca tttccgctga tcgcgccgcc aattcggccc
     2881 cgtgcagccc gtaaagccga ccctggtggc ggaggttttc catcacggtc aattttttgt
     2941 ccaggctggg cgattggaac acgacgccca tcttctgccg cgcggcgtcg ggggcggacg
     3001 tgacgtccgt gtcgaacaac cgggcgcccc cccccgttgg acgaagcgcc gtggaaagga
     3061 tgcgaaaaag ggtggtcttt cctccgccgt tggggcccag aacgccgaaa agttccccgg
     3121 gggagacctc gaaatcgatg ccctgaaggg ccacccgcgg gggccccccg gggtttttgt
     3181 attcgtggcg cagaccgtcg acccggacgg cgggattcac agggtccccc ggcggtcaag
     3241 caccagaatc aacagaagca acgggaggta gagcacggaa gccaggaaaa cccgtcgggc
     3301 tcgaaggtcc gtgtgttggc ggaaaaagac aaaggacaac gccagaagcc ccaggcccat
     3361 caagaacgcc gccgcgaaat aaatcgtgcc ggccaatccc aggaaggtgg gcaacagact
     3421 gacgctcgtg agggccaggg tcgtcaccac catgtgccgg gccgtggtct ccccgtcggg
     3481 ctccacggcc ggaagcatgg ccaaacccgc ccgggcgtaa tcggcccggt aaagccaggc
     3541 cagggagaag aaatgggggt attgccagaa aaacaaaatc gcgaagagga cccaggcccc
     3601 ggggtccaaa cggccgacgg cggcggtcca gccgatgacg ggcggtaaag cgccggccac
     3661 cgcgccgaac agggtgttca gggcggaaat tttcttcagc ggcgtgtaga cgtagagata
     3721 aacgcagagg gtcaggccgg cgatcacggc ggtgaggacg ttcaccgccg ccgaaaggta
     3781 aaggacgccc gccagggcca gaagggaacc cagaaacagg gcctcccccg cgttcaagcg
     3841 acgggccggc aggggccggt tttcggtgcg atgcatgcgc gcgtccacgt cccgctccaa
     3901 aagcatgttc aaggcgcagg ccccgccgga aacgagcagg gtgcccacga ccgtgtgcaa
     3961 cagccgggcg ggcccgacgg cgcccccgga cccgaggaaa acccccgcca gggccgtcac
     4021 ggcggccatc aaactgatgc ccggcttggt caacgtcacg taatcccgaa aacgtccgaa
     4081 ggccacgggc cggccccgcc aggacagaag ggtcaacgcg gccgaaaggc ccaggaggag
     4141 cgcccccgtc atgacgtggc cggtggccaa caggacggga cgcaacgggc tggagatcca
     4201 ttggggcacc aggagcgaca gaagagtcag ggttccaaga acgatctgaa ccacgtaaac
     4261 cagggccaac gccgacgcca ggcgccagag ccgggggtcg gtttcgtcgc ccaaggccag
     4321 ccgccgaaac acccagccca aacccagaaa aatcaacacc gcgttggcga tgtgaaggcc
     4381 cgccgcgtga ccggtgtgcc gcaccacggc ccccaaaacc aattgggcgt agagggtcag
     4441 gaacaagagg agggccacgt ggggcaacgc cacgttcacc ggatcgccgc ccgcggaggg
     4501 gaaacgctcc ttccaggccc gggaggtcca taccaccagg gcgaccgaca gacaaaaaaa
     4561 ggtttgcgcc aggcaggcgt gggtgatgga cacggccttg ggaaggcgga gcaggaccgt
     4621 caagccgccg aagaccccct gaaggacgac caatcccaag gccccgatgg ccgcccgacg
     4681 aacccaggcc cgggactcgg ttttccagaa cacgaggttc aagacgatgg tcaacagacc
     4741 gacggaggcc gccgccaatc gatggccgtg ctcaaaaagg attccgccga tcatgggcgg
     4801 caggaggcgc ccgtaggaca gcggccaatc cggcaccgtc aggcccgaat gggtgctggt
     4861 caccaacccg ccgataaaaa caagcgccag ggtggcggcg gccaggaccc acgccaatcg
     4921 gtggggccag ggactcggaa cggcggatgt ttcggacatt gacatgaagg aaaatcccct
     4981 ccggggattt actgaggcag ggggtgcgtg ggacgttggc ctttgcggat atgatacacc
     5041 accaaggcca ccagcgaacc cattaaaagg aacggaagga ccaacaaaag gagaatggcc
     5101 caaaagaacc cccggttgag ggccgacccg ggagctccga aacacaccgc gcaggcccgg
     5161 gcgttgacgg cgatcatcga gaccaacgcc gccgccggga gttttctcac ggccgggcga
     5221 cgccgatgtc cgggagaacg gccaagacca ggacggccac caggaacaat cccgtgaaga
     5281 gcagggcccg aatcgccttg ccctcccatt tcaaatgcat gaaaacgctg gcaatcaggg
     5341 tgcatttggt gagcgcgatc aaggcggcca ggggaatggc cacccggtga gacaggtgga
     5401 ggtagctgag caataccgtg acgccggtga gaagtgccag accgccgaac accgccgcgt
     5461 aaaggggaac cggggatttt tggttgtcca tggctagacc aggtagaaca tcggaaacag
     5521 gatcacccac accaaatcga cgaaatgcca gtagaggccc gcgtattcca cgcgctcgtg
     5581 gtgccgggcg ctgtagcggc cccgaagccc gttgcggagg acgtagccgt tgaaaacaag
     5641 accgccgatg atgtggagcg cgtggaggcc cgtcagcgtg aaataaaagg agccgaacag
     5701 gccggaggaa atcgtgaggc cctcggaaat cttgtggtgg tattcaaaca ttttaacgat
     5761 caggaaaaga attcccaggc ccatggtggc gatcatgttc cgggcgaagg atcgccggtc
     5821 gtcccgctgg atggcggcca aggccaggac catggtgaaa ctggacagga tcaggaggaa
     5881 ggtgttcacc gtggccaggg gccgacccat gacctcctcc gacggaacgc cgaaattggg
     5941 gcttcccatg cgcaggacga tgaaggcgct gatgaaggcg ccgaagagca tgatttccga
     6001 cgacaagaac acccacatcc ccagtttgcc ggagggaatg ggcgtcgacg gcggaaagga
     6061 cggcgcggcg gattcggaca tcagtttttc tcccattggg gccaaaaatc cttggcgcgg
     6121 cccggaacgc tgtattcgta gggcgcccag tgggccgaag gaacggaggg gaaattgccg
     6181 tggggcggcg gcgacgggca ggcccattcc agggtcgtgg cgtcccaggg gttttcccgg
     6241 gcgacaacgc ccttcgtcat ggatttaaag aaattataga ggaaggggat ctggaacaac
     6301 aacagcagga tcgcggacgc cagctggtag gggcttaaaa actgggtggt caggttatgg
     6361 gtttgaaccg tcggatcgta gaggcgccgt tgcatgccgg ccaatccctg aatgaacatc
     6421 gggagaaaga tgccgttcat gccgatcaac gtgccccaaa agtggatttg gcccagccga
     6481 tcgtccatgg agcggccgaa cattttcgga aaccaatgat aaacgccggc catcaaggcg
     6541 atgatggttc cggtgacgac cacgtagtgg aagtggccga tgacgtaata cgtgtcgtgc
     6601 aaatagatgt ccgtcggcag gaagcccagg ggaagcccgg tgaggccccc gatgccgaac
     6661 atggggagga aagccagggc gaaaagcatg ggcaccgtga aacgaatctt tccgccccag
     6721 aggctgaaga gcaggcaact caggatggcg acggagggaa tggaaatcac catggtcgtc
     6781 gtcataaaga acatgctgag ggaggttttc attccgctca caaacatgtg gtgggcccac
     6841 acgagaaagg agaaaacacc gacgaagatc atggagccga ccatcgtcgt gtagccgaaa
     6901 agcggtttgc gggtgttggc ggcgatgatc tccgacacga ttcccatggc cggaaggacc
     6961 aacacgtaga cttccgggtg ggccaaaaac cagaacaggt gctgccagag cagcgggctg
     7021 ccgccgccca cccggtccaa cgtttttccg ccgaccacca accccatggg aaggtaaaag
     7081 caggttccgg ccacccggtc caacaactga aggatggccc ccgattgaag aacgggaaac
     7141 gcgaacagca gtagaatcgc cgtgaccaat tgggcccaga caaaaagcgg cagccgggac
     7201 caggtgagcc ccggcgcccg caggttgacc gtggtgacga tgaaattgat ggaccccagg
     7261 agcgaggaag tgatcaggaa cgtcatgccg atcaaccaaa tcgtctgacc cgggttgatg
     7321 tccgacaggg gcgggtagga ggtccacccc gattgggcgg ggccgcccgg gaggacgaag
     7381 ctcaggagca tcacgacgcc gcccatggcg taaacccaat aactgctcat gttgagccgc
     7441 gggaaagcca tgtccggggc gccgatttga aggggcaaga aatagttgcc gaagaagccg
     7501 acgcccaggg gaacgacccc caggaaaatc atgatggttc cgtgcatggc gcccaattga
     7561 ttgtagaggg ccggcaacat gaccccgccc ggggcaatgg cgtccggcag gaggccgccg
     7621 atcaagggaa gcgggcggcc gggaaaggcc agttgccagc gcattaatag gatgaaggcg
     7681 aagcccagaa ggaggaaggc catggaggtg aagaggtact gcaacccgat caccttgtga
     7741 tcgaggctga aaacgtaagt gcgccaaaag cttttttgtc gaggctcttc gtgcatgagg
     7801 gggtttactc cgaccagtcg ctggcggcga ccactttggg tttcgcggcg gccttggcct
     7861 gtgaaacgcc ggcccaccat tgggcgtatt cttcgggggt ctgcaccacg atttcgcccc
     7921 gcatcacgta atggcccgcc ccgcagagtt gggcgcaacc gatttcaaaa cggccgaccc
     7981 ggttgggttc gaaccacagg ggaatcttca tgccgggcac gacgtcctgt ttcacccgga
     8041 actccggaac aaaaaaactg tggatgacgt ccttggacgt catctgaaga atggtgggct
     8101 tgcccagagg gacgtgaagc tcgttgtaaa gcacgaggtc gtcggccgcg gccgggtccg
     8161 tgtcgtccag gccgaggggg ttggagaccg tcacccgcgc ggggtcccgc cgccccaatt
     8221 ttccgtccgg gccggggtaa tggaatcccc aggaaaactg ctcggcggac aatcccacga
     8281 cgttggaggc gtcctccgcg ggaaaatcct gcttgatgtg ggaccaaatg ggaagcccga
     8341 aaacgaagat caaccacaat tcaaacacga ggaccagggc gtcggggacg aaggacgagg
     8401 cgtggccctt ccacccctga taggacgccc ggtcgacttt cttttgctgg aagcgccaaa
     8461 ggcagtagac gaaaaacgcg agccaaagaa gaaagatcgc gatcatgccc acgtggagga
     8521 ccgtgaggga ggtgtcgacc cgcccggcgt acgtcgaggc cgccaccggc aaacccacgc
     8581 gataatttcc cagcaattcg tccatgaggg gggtcgacgg gagccttact cgctccagtc
     8641 gtccttggcg gcggtggccg gccgcaagga gtacacgtat tccaccaaat cgttgatttc
     8701 atcatcggtc aggtattggc cccagggcgg catgcggatc atcggcggcg gctgggacgc
     8761 gtcggccgga agggacaccc ggcctttgtg aagcaagtcc cgcagttcgt ccttcgagta
     8821 tccatcggcc gcgtaggtga gggaaaggat tttcccgccc acgacgttgt tgttgggata
     8881 ccccccctgg ccgcgttccc cgtggcatcc cacgcacccg acccgggcga acaacatttc
     8941 gccgcgtttc aaggggtcgg ccttgagggc gggattgtcc caggggggct tggcgcggta
     9001 gatttcgccc ttgagagagc tcaagtaggc caccaggtct tcccggacgt tgtccttaag
     9061 tttgaagttg ggcatggccg tgccgggctt ccaggcggcc ggattcgcga gccaaaggcg
     9121 cagccattcc ggggtcttcc ggaaaccgac ggtggtcaaa tcgggtccgg cctggccgcc
     9181 gcctttgtcc ccgactcggt gacaagtgat gcatcccaag ccgacgaagt aggaccgtcc
     9241 acgggccacc gggtctttgg accccgagca acccgcgagc aacaccagca gcaagagaac
     9301 cttgaatcgt gacattggaa ggagggattc tataatctcc caacccctcc ggcaagagcc
     9361 cttgaaaaat tcggattctt ctgctatgtt gattttacga tgaccccaag agattcctcc
     9421 cccctgggct ttcgccggat ccgtcgcttc ctcgccgcgc cgggcgtgtt gtttttggcc
     9481 ctgatcggtc ccgcggccgt ccccgcctgg gccgacatgg acgacaaccc ctcctatttg
     9541 aaagactgga ccgaacggga caagggttcg gggtggaatt tttccccggc gttcaacggc
     9601 ggctacacct ccctgctgca cggctacaaa gggtacaaga acctcggcaa cgccggcctg
     9661 gatctttaca tgcgcccgcc ggtgccccaa tttccccgct ggtataatca catgattttt
     9721 cgattgtcgg ccgattattt tccccttcaa gtcccttccc aggtggtggg gatcaccgag
     9781 gacatctacg ccctgaccgg cacggcgctt tggcgctttt caaacgccag cgaagccgaa
     9841 cacaaccaat ggattccctt tttgggtgcg ggggtggggg tttactggga ctccgttacc
     9901 gtggacacgc tcgccaccgg aaaggtgaaa gacgctgagc aatatttggg ggtgacgggc
     9961 gccctcgggg tgatgttgcc gacctggggg ccgattcgca tcatccccga aattcggtat
    10021 cacactcttc gcgaagccgg acgatattgg gcctcccaca tgacgtacca aatcggcgcc
    10081 gctctttggt tcccggcgaa agtgagcgaa caatgaaccg cccccttccc cgcctcgcgg
    10141 cggtggtcct ggccctgttg gccgtgggag gctgcaaacg aaaggacaac aatccattgg
    10201 tcggaacacc cggcgcgggg gtgaccggcg tgaccttggc cgtccggggc gtggacaaca
    10261 cccaatttcc cgacatcaaa gtcctcctgc aacccaaaac cagcgggggc gcccccatta
    10321 ccgatttaac cgttggaaac ttccaaattc tgcagggcgg caaagcggga ccgatcccca
    10381 cggggtacga atcggcctcg ggttacccgt tttcggtcat gctggtgatc gatcacagtg
    10441 gaagcatgtc ggcggacgat ggcacggggt cctcccgttt ggcccgggcg caaacggcgg
    10501 cggcggcttt tttgggggct ctttcgtcga atgacgaagc cgcgttcatc gaatacgacg
    10561 atagcgtcaa cttcgtccaa ggttttacca aggacaaaaa caccgtcatc gccgccgtca
    10621 acgccagcac cccggccggc gccacggcca ccttcgacgc cattgtccgg ggcgcggagg
    10681 aattgagcaa aggcaccggc cttcggcttc ttgtcttcct gaccgacgga gacgacaatt
    10741 ccagttcgaa caccgccgcc accgtcaaaa ccaagctgtc ccaggcgggc gtcgtggcct
    10801 gcgggatcgt gatcgccggc ggagcgaccg acacggccac gttggacgac atcgcccaaa
    10861 gcaccggtgg acaacttctc gcgggaacga ccacggtgga gcttcagacc gccttcaaca
    10921 ccatattgtc gtcgggactt ttcgacgaca tttacggcct cagttttcgg agtaaaaacg
    10981 ggcagggcag cgccacctac agcgtttaca tcaactacgg ggcctttacc gaccgggcgg
    11041 atttaatcga cttcctttaa cctcccccct tccattagaa aaaaacccgc ctttcgaaat
    11101 gaaaggcggg tttttttatt ctttaaaaag cgcgttcagc cgtatttgta ctgcaccccg
    11161 aagccgcccc ggggccaatg gcagtcgatg ttgtcgaagg ggcagagcat gcggcaggcg
    11221 ccgcattcga tgcagttctc aaaggagacg atgattttct tttggtcggt ctcccaccga
    11281 tagacctggg cggggcacac cgtggtgcaa ggcttgccgt cgcatttttt ggcgcagacg
    11341 tcggcggatt tgatcgtgat gtgcgtgtgg tggtcggcct tgtaggttaa ggtgcccagc
    11401 ttttcgtcga cgttgatctt ggggaggtcg gtcatatcgg ggtcagttct cgtgggtcaa
    11461 ttttcggaat ttgaggcccg ctttgaggcc cattttcaac aaattgtagg cgttgcccca
    11521 ggtcatttcc gaggcgatga ggcttttg
//
LOCUS       DAOQLS010000003        10736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_2373_length_10736_cov_5.606966, whole genome shotgun sequence.
ACCESSION   DAOQLS010000003 DAOQLS010000000
VERSION     DAOQLS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2373_length_10736_cov_5.606966"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>10736
                     /locus_tag="PK362_00135"
     CDS             <1..>10736
                     /locus_tag="PK362_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73282.1"
                     /translation="DAARVKAAPAGLRQWMLRNYLLLKQGQFPRYNTYGAHGRYISFI
                     GLSEIKRMTARVEKSDETLAALRNRARELDKQIKRLAFDRRTLASEAQRRELAELNRA
                     RDQIDTDVVVRRTILQRRFVDALRQSLAERRDGVLGEAGDSLREQRDVMWRTFDGTFA
                     YYESRLVPEGFQLQTGARLFLTLAAIGRLAFTGIFLSQVFFFPSAALTAASWVVLGPL
                     LYNISRSFVDPLLVMVFMMFGPPPQGHARRDFLEVVREHAQKTGRPYAFSVEVPKMSG
                     NPLDGYLKTDIGAAYFKSANGLSASFNLNPTAPSASLQFPRVLDEAAQAEFARFVQGK
                     MWEGFRLDVEVTFQEARDAKNAVIPGAMVAVLRPSPGTAGRRFETRVNEILSAAKGET
                     QQLSPDGVRVVGNEWIEVDVTENYVNPVGVGFPEIGGPTTAEEKTWAGLGKMIEKEYS
                     LPLSLAADREPNITVRFDEVDGRVVRRVVYRFGVPRQYRSEIPQNFDPTTRFLDTIHL
                     EADVWAAYRRVDIDNLRQTLRDTAVEPGAPEVFTISFAMPTNTSNPQLIPYELENLQR
                     LQAEMDAEFPGRVSFVYFNQGMEWAESFLAGPAAVSAVSGAIGDADPLKKLGNQFTLF
                     DKEGARLYVTVQGNAVTTVQVSAQGRRTVRTLTRAEATALIKGVDASAVDRVVQWKRG
                     GWGKKLGNVAGLEWLKAGHTRPPAYNNPDGHENFTNPDAPIFPNANLMAGDILGYFRN
                     DVVVHRADGTTVRFTGSTTDANQITVAESRGESRTMTRAEANKELAQRIADLHTDRMT
                     DGEGRPLEPDDLSVVMDDKNSILPGAVEAAIGVAEHPANQGVVGFNPAIEVDPPRNTR
                     GGQAGSYFSSILLDYLDKSRVIHNMYDARFKAGLFGKMSAMYGKIFKRAAYGDSFTDE
                     RVTVARALSHDWQESQFSPTEAVYGDVTHTFQLVESSRNEDTREAVQVFRRQLGNRFE
                     LLRLVLSPVADHPGQINATVERLTPEGQWAVWRTFPAAASESAKVLVTRVAAFLTNAI
                     GVRERDLLTLAGVVDRDFRWLRGDLQMQSTAKPYEKDALPHAHRYHLGGIDFRLNGDP
                     SFLIVLAGLIPLWLFPDQALLVNKTLAYVLFGITMLGVAFRGHFLYPAYFDAMSRVQV
                     LTVSPWLGKPLLWGLLLVFGVKNLLLSLLTISATTLMSLPIAIKRTFYLETTAKVIEF
                     VKNVPSVWVTSSATVVRELMGVKIADTWKSEISLMNLVVFGGLSFAAFAGLIYTGNIY
                     FGALFPLGISIIVASILTGWIVAHFAGQAYRRDDRSAMMKRWFQVYGLSALIAAFIFF
                     APVTNFLTDLKIQSLRTPTAAEVVLKQLADPVTKPRPYTGVDISGGLALQVQRTEPIA
                     QEAAPKIMQAAVNVGAGVKAEVFPGVESIRLTGIPNTALPLPGDVRTSPYTTAQFEPP
                     KPLDMLKKPQLTEEQTKKLAYLLSQLDRMGDDTTKWENIAIYGGSTALITLVPPSAIG
                     LKVADVGAYLKMLSTGYPWLREPMVAKYWAQTALLMDINATLGDAKTAKAAQVRSVKD
                     ISQLLLWDKIKDVWDVDYTRFVQKGQVLRNPKTGGVLISEEDYRDLAETASGSMDKYE
                     VLSTAVINLHYGLGYPLEKITPELVWRFTRLSYEIEKSWAVVVPSVDIKPVQLFPKSV
                     GKVLRVAGNITFFLPVKIPILSDAHLEMIPMVTAFYQEPGGLQDFFTQMLEHNERDLR
                     GAGNEDYIKAFLDRIGADYIQRFSDPAKFPVDSALYNIVRDRMTEDLKKFNKKPTDKD
                     LRLAYLQTEYNLGRIALFNRHKVESKDLDAIRAAALSVARELSTVQSIAKSLAPRLYE
                     SHMKMEPGVLEVEGIMLYLLQGEPDRKVEYLRVFRDVLKTSEAILADMAKNKWTVSDA
                     YSQHVLSLLPPALVADMSPKAKEYNVLRDLSMLQMNIQLNGIKKADGKPYAFKEIMDA
                     LLAVKARVAGLRVPEKPGIMATMLLHSLKMKTQHPEVTDKEYWEGNRLPVLRYWAAWS
                     ATPELMKKLDADPHWAWYRNKINGEIEAEMGAKITDPEVRQFNAVQSMAQLADAVYNA
                     FPNLKKEHQGKPARVADLLLTLAHRSETYTQFFGTLKNMPVNAIGFQEYFVVAGYELN
                     GMSQKFDPKKAFEKEIDGFVLRPVNDLWNTGLYESLPKAFVEAVRVRMKDTQERLGIG
                     DRPIKADDLKIQAIQAFYKMTTSAMFSETYPAPLPAYSAAFLKDVVALGRELVGYAVE
                     IGEQTAGLPRVNEYVVKEPGILENEAIQIYLLKKRSGANFNLKNYVAFTRAIFLKIDS
                     ILRDQPGLLSSATFAKYRTVKSSLLSEAANKQMSDRGRDFLALRSIANLVVNAEHNDM
                     RPKAPLAARALSAEGWMTAYLDAWDHFENAPKYGGKKYQHIPYREEGFVDMVTLLKQK
                     WGFTKFWEAYYYERLNQVEKFMSDPAFVKFIRTQGGTGEKLLWVINDDIFRKSGIRFD
                     SNHPIFTFNGVIGVTEMFIDAAKKFPGTEINYQLLATRYLRYYDLFHQPDPATGKPRF
                     PNLPYMEEGFIESLVTGGYNFEANGGRFEDELRIMKLRDVDALMGAKLLDPEEKFQGI
                     FVRGYLEGIIRSESAVPPGVSAPVIDFFILQKIVGIGQTYNMLTGPSVGVGQPSSVDV
                     QKDLPVLKTATRALVFVHNGVRQQTADKKERYPKLRPIFNILGESEKWVGRMHATGLL
                     KEDRFQEIFTNYIDVLERHFDELKGTYLPPEQGAGGARYLGELGTYLRVLKENLRKDA
                     AAIESRFVTRDQQTRQLGQAAGLEEEKAGGTEAETRARVVFVRPEIDESQFILDAGYI
                     VTEILRDMEREIGASGNAALKNVTVDRLMTHYFKTLAHLKTTHYKNQIMQIQSYRDSE
                     ALGRLNGKINNYAMRFAFIDYLVYSAEGDPNLRAFFESKGLNSAQQFIDRFYANLEIV
                     QKTDGLKQALEELRNANEYSAEGVEFSYAMMLTLNHKITPETLKRVATHIPAVLRDYN
                     DYLLRPGAAAGQSLKFRVDSAVVLYEALARELALARPFGATGDRMEVKQKRTNEPWMV
                     DTLVKAVYGNLFGIYLEDDNPEHRKLMKEWEIKAFGKDLKSMVQQLASEPTVMGQKNA
                     YFDEIAKVDEAIRERTAKYEAAKRDLRESLADDYQIQIRILEQIRASLEERMGYLLQL
                     ADTMDQTYAEGVKDKNQATLEVLILLSALGFGAGLLAFSRRRWDRSSREGLTRLQRLR
                     ADLADLNAQRKQVPYLSNAALAIYLGLPAAYGLYFGVGAPRLAQDAGSIFTQAPYQFE
                     SPRSSQGTLFSEGGFSVRQSQEVRDGKPVVEHKILSGGYLVGRIELNTGYAEFMPGAV
                     PGGNGFSYPLNPGPSLVMLGFGENKPDGKKAYSYRGALSQVTYSVDSNGRLVLSGTLR
                     DAGGAFEARNYQMAVSVTAGQTRVSVSGDFTARRDLNVSEFNIGSLNTQSYIGVPQGA
                     PSPSDRSLSLEAQGTVAGNRVYLYFGNDHKLPDSGLTPWTVQSVSLGGTAYNNTPNVR
                     IVPESITFNGRKVDPVAAYTLLPNQFDRHNVAIAPVIGGGTPLTLQTGGKISVR"
BASE COUNT         2297 a         3368 c         3062 g         2009 t
ORIGIN      
        1 gacgccgccc gcgtcaaggc cgcccccgcc ggccttcgtc aatggatgtt gcggaattat
       61 cttctcctta aacagggtca attcccccgt tacaatactt acggcgccca cggccggtac
      121 atctccttta tcggactttc ggaaatcaag cggatgacgg cccgggtcga aaaatccgac
      181 gaaaccctgg ccgccctccg gaaccgcgcc cgggaattgg acaaacaaat caagcgtttg
      241 gctttcgacc gccgaaccct ggcgagcgag gcccaacggc gggaattggc cgaattaaat
      301 cgggcccggg atcaaatcga cacggacgtc gtcgttcgcc gaaccatact tcaacgccga
      361 ttcgtggacg ccctccgtca atccctggcg gaacgacggg acggcgtcct gggggaagcc
      421 ggcgattccc tgagggaaca gcgcgacgtc atgtggcgga ccttcgacgg aaccttcgcc
      481 tattacgaat cccggttggt ccccgaaggc tttcaactac aaaccggcgc ccgcctgttt
      541 ctcacgctgg ccgccatcgg ccgcctggcc tttacgggca tattcctctc gcaggttttc
      601 tttttccctt ccgccgcttt gaccgcggcc tcttgggtgg ttctcgggcc cctcctttac
      661 aacatctccc gctccttcgt ggatcccctg ctggtgatgg tgttcatgat gtttgggccc
      721 ccgccccaag ggcacgcccg ccgggacttc ctggaggtcg tgcgcgagca cgcccaaaaa
      781 accggtcgac cctacgcctt ctccgtcgaa gtgcccaaaa tgtcgggcaa ccccctggac
      841 ggctatctaa agacggacat cggcgcggcc tatttcaaat cggccaacgg cctttcggca
      901 agttttaacc tcaatccgac ggcgcccagc gcctcccttc aattcccccg ggtcctcgac
      961 gaagcggccc aggcggaatt tgcccgcttt gttcaaggga aaatgtggga aggattccgg
     1021 ctggacgtcg aagtgacgtt ccaagaagcc cgggacgcga aaaacgcggt aattcccggg
     1081 gcgatggtgg cggtgcttcg cccctccccg gggaccgccg gacgacggtt cgaaacccgc
     1141 gtgaacgaaa ttttgtccgc cgcgaaaggc gaaacgcaac aattgtcgcc ggacggggtc
     1201 cgtgtggtcg gcaacgaatg gattgaagtg gacgtgacgg aaaattacgt caaccccgtg
     1261 ggcgtcggat tccccgaaat cggtgggccc acgacggcgg aggaaaaaac ctgggcgggg
     1321 ctcgggaaaa tgatcgagaa ggaatacagc ctccccctgt ccctcgccgc ggatcgggag
     1381 cccaacatca ccgtccggtt cgatgaggtg gacgggcgcg tggtccgccg cgtggtttat
     1441 cgctttggcg tcccccgcca ataccgaagc gaaattcccc aaaacttcga cccgaccacc
     1501 cggttcctgg acaccatcca cctggaagcc gacgtgtggg ccgcctatcg ccgcgtggac
     1561 atcgacaatc ttcgccaaac cttgcgggac acggcggtcg aaccgggcgc gccggaagtc
     1621 tttacgattt ctttcgccat gccgaccaac accagcaacc cccaactcat tccctacgaa
     1681 ttggaaaacc tccagcggct tcaggcggaa atggacgccg agtttccggg acgggtcagc
     1741 tttgtttatt tcaaccaagg catggaatgg gccgaatcgt ttttggccgg ccccgccgcg
     1801 gtttcggcgg tgtccggcgc catcggggac gcggacccgt tgaaaaaact cggcaaccaa
     1861 ttcacgcttt tcgacaaaga aggcgcgcgg ctgtatgtca ctgttcaggg gaacgcggtc
     1921 accacggttc aagtcagcgc ccagggccgc cggaccgtcc gaaccctcac gcgggccgag
     1981 gcgaccgcgt tgatcaaggg cgtcgacgct tcggcggtgg atcgcgtcgt gcaatggaaa
     2041 cggggcggct ggggtaaaaa actgggcaac gtggcggggc tggaatggct caaggccggc
     2101 cacacccgcc cgccggccta caacaatccg gacggtcacg agaatttcac gaacccggac
     2161 gcgccgattt ttccgaacgc caacctcatg gcgggcgata tcctggggta tttccgaaac
     2221 gacgtcgtgg ttcatcgggc ggacgggacg accgttcggt tcacgggatc aaccaccgac
     2281 gccaatcaaa tcacggtggc tgagagccgc ggcgaatccc gaacgatgac ccgcgccgag
     2341 gccaacaaag agctggccca gcgcatcgcc gatcttcaca ccgaccgtat gaccgacggg
     2401 gaaggtcgcc ccctggagcc cgacgatttg agcgtcgtga tggacgacaa gaactccatc
     2461 ctcccgggcg cggtggaagc cgccatcggg gtggccgagc atccggccaa ccaaggcgtg
     2521 gtcgggttca acccggccat cgaggtggat cccccgcgga acacccgcgg cggtcaggcc
     2581 gggtcctatt tttccagcat cctgttggat tacctggata aatcccgggt gatccacaac
     2641 atgtacgacg cccggtttaa agcgggtctc ttcggcaaaa tgtcggccat gtacggaaag
     2701 attttcaaac gcgcggccta cggggattcc ttcacggacg aacgcgtcac cgtggcccgg
     2761 gccctttccc acgactggca ggaaagccaa ttttcaccga cggaagccgt ctacggggac
     2821 gtgacccaca ccttccaatt ggtggagtcc tcccgcaacg aggacacccg ggaagccgtt
     2881 caagtgttcc gccggcaatt gggcaaccgt tttgagttgc tgcgtttggt gctctccccc
     2941 gtggccgacc atccgggaca aatcaatgcc accgtggaaa ggttgacccc ggaagggcaa
     3001 tgggccgttt ggcggacgtt cccggcggcg gcctccgaaa gcgccaaagt cttggtcacg
     3061 cgcgtggcgg cttttctgac caacgccatc ggggtgcggg aacgggacct gttgaccctc
     3121 gcgggcgtgg tggaccggga ctttcgctgg cttcgcggcg acctgcaaat gcaatccacc
     3181 gccaaaccgt acgaaaagga cgccctgccc cacgcccacc gctaccattt ggggggcatc
     3241 gattttcggt tgaacgggga cccttccttc ctgatcgtgt tggccggcct gattcccctc
     3301 tggttgttcc ccgaccaggc cctgttggtc aataagaccc tggcctacgt gctcttcggg
     3361 attacgatgt tgggcgtggc cttccggggc catttcctct accccgccta ttttgacgcc
     3421 atgagccgag tgcaggttct caccgtctct ccgtggctcg ggaaacccct tctctggggc
     3481 ctgcttctcg tgttcggggt gaaaaacctg ctgctctccc tgctgacgat ttcggccacc
     3541 accctcatgt cgttgcccat cgccatcaag cgaaccttct atttggagac caccgccaaa
     3601 gtcatcgaat tcgttaaaaa cgtgccctcg gtgtgggtga cctccagcgc caccgtcgtg
     3661 cgtgagctca tgggcgtcaa aatcgccgac acctggaaaa gcgaaatttc gttgatgaac
     3721 ctggtggtct tcggcgggtt gagcttcgcc gctttcgccg ggctgatcta caccggaaac
     3781 atttatttcg gagccctctt tcccttgggc atttccatca ttgtcgcctc catcctgacg
     3841 gggtggatcg tcgcccactt cgcgggccag gcctaccgcc gggacgaccg cagcgccatg
     3901 atgaagcgct ggttccaggt ttacgggctt tcggcgctca tcgccgcctt cattttcttc
     3961 gcgcccgtga ccaatttcct caccgacttg aaaattcagt cccttcgaac gcccacggcg
     4021 gccgaagtcg tgctcaaaca actggccgat ccggtcacca agccgcgtcc gtacaccggc
     4081 gtcgacatct cgggcggctt ggccttgcag gtccaacgaa cggagcccat cgcccaggag
     4141 gccgccccca agatcatgca ggcggcggtt aacgtggggg cgggcgtgaa agccgaggta
     4201 ttccccggcg tcgagtcgat ccgcttgacg ggaattccca acacggccct gccgttgccg
     4261 ggggacgtcc gcaccagtcc ttatacgacg gcccagtttg agccgcccaa gcccctcgac
     4321 atgctgaaaa aaccccagct caccgaggag cagaccaaaa agttggctta cctgctctcc
     4381 caattggacc gcatgggcga cgacacgacc aaatgggaga acatcgcgat ttacggcggc
     4441 tccacggccc tgatcacgtt ggttccgccc tcggccattg ggcttaaggt cgccgacgtc
     4501 ggcgcctatc tcaagatgct ctccacgggc tacccctggt tgcgggagcc catggtcgcc
     4561 aaatattggg cccaaacggc cctcttgatg gacatcaacg ccaccctggg agacgccaag
     4621 acggccaaag ccgcccaggt ccgttccgtc aaggacatct cccagctcct gctctgggac
     4681 aagatcaagg acgtttggga cgtggattac acccgattcg ttcaaaaagg gcaggtcctt
     4741 cgaaacccca aaaccggcgg cgttctcatt tccgaggagg attaccggga cttggcggaa
     4801 acggccagcg ggtccatgga caaatacgaa gtcctttcca ccgccgtcat caacctgcat
     4861 tacggtctcg gctaccccct cgaaaagatc accccggagc tcgtgtggcg gttcacccga
     4921 ctttcctacg agatcgaaaa atcctgggcg gtcgtggttc ccagcgtcga cattaaaccc
     4981 gtccagctgt tccccaaatc cgtcggcaaa gtgctgcggg tcgcgggcaa catcaccttc
     5041 tttctgcccg tcaaaattcc catcctttcg gacgcccacc tggaaatgat tcccatggtg
     5101 acggcctttt accaggaacc gggcggcctt caggatttct tcacccaaat gttggagcac
     5161 aacgaacggg acctgcgggg ggcgggcaac gaagactaca tcaaagcgtt cctggaccgg
     5221 atcggcgccg attacatcca acggttcagc gatcccgcca aattcccggt ggattcggct
     5281 ctgtacaata tcgttcgcga ccggatgacc gaagacctga aaaagttcaa caaaaaaccc
     5341 acggacaagg acctccgtct ggcctacctc caaaccgaat acaacctggg ccggatcgcc
     5401 cttttcaacc gccacaaagt cgaatccaaa gacctggacg cgattcgggc cgccgcgctt
     5461 tccgtggccc gggaattgtc cacggttcaa agcatcgcga agtccctggc gccgcgcctt
     5521 tacgaaagcc acatgaaaat ggaacccggg gttttggaag tcgaagggat catgctctat
     5581 ctcctccagg gggaaccgga ccgcaaggtc gagtatttgc gggtgttccg cgacgtcctt
     5641 aaaacgtccg aggccatatt ggcggacatg gccaaaaaca aatggaccgt gagcgacgcc
     5701 tattcccagc acgtgctttc cctcttgccc ccggccctgg tggcggacat gtctcccaag
     5761 gccaaggaat acaacgtcct gcgggatctc tccatgcttc aaatgaacat tcagctgaac
     5821 ggcatcaaaa aagccgacgg caaaccctac gcttttaaag aaatcatgga cgccctcctg
     5881 gcggtcaagg cccgcgtggc cggtcttcgg gtgccggaaa agcccggcat catggccacc
     5941 atgctgctcc actccctcaa aatgaagacc cagcaccccg aagtgaccga caaggaatat
     6001 tgggaaggca atcgcctgcc ggtgcttcgc tattgggcgg cctggagcgc cacgccggaa
     6061 ttgatgaaaa aactcgacgc cgacccccac tgggcctggt atcggaacaa aatcaacggg
     6121 gaaatcgaag ccgaaatggg ggccaagatc acggaccccg aagtgcgcca attcaacgcc
     6181 gttcaaagca tggcccagct ggcggacgcc gtgtacaacg cgttcccgaa cctcaagaag
     6241 gaacatcaag ggaaacccgc ccgggtggcc gacctcctcc tcaccttggc ccatcggtcc
     6301 gagacctaca cccaattttt cgggacgttg aaaaacatgc cggtaaacgc catcggcttc
     6361 caggaatact tcgtggtggc gggctacgaa ttgaacggca tgtcccaaaa attcgacccc
     6421 aagaaagcct ttgaaaaaga gatcgatgga tttgttctcc gtccggtgaa cgatttgtgg
     6481 aacacgggac tctacgagtc gcttcccaag gcgttcgtgg aggccgtacg cgtccggatg
     6541 aaggacaccc aggaacgcct cggcatcggc gatcggccca tcaaggcgga cgatttgaaa
     6601 attcaggcca tccaagcctt ttacaaaatg acgacctccg ccatgttcag cgaaacttac
     6661 ccggcgcccc tgccggccta ttccgccgcc tttttgaagg acgttgtcgc cctgggccgg
     6721 gaattggtgg gctacgccgt ggaaatcggc gaacaaacgg cgggattgcc ccgggtcaac
     6781 gagtacgtcg tgaaagagcc gggcatcctg gaaaacgagg ccattcaaat ttatttgctc
     6841 aagaaacggt cgggggccaa tttcaacctg aagaattacg tggctttcac ccgcgccatc
     6901 ttcttgaaga tcgattccat tttgagggac cagccgggcc tgttgagttc ggcgaccttc
     6961 gcgaaatacc ggaccgtcaa gtcgagcctc ctctccgaag cggccaacaa acaaatgagc
     7021 gaccggggcc gggacttcct ggccttgcgg tccatcgcca atttggtcgt caacgccgaa
     7081 cacaacgaca tgcgccccaa ggcccccctg gccgcccggg ccttgagcgc cgaaggctgg
     7141 atgacggcct acctcgacgc ctgggaccac tttgaaaacg cccccaaata cggcggcaaa
     7201 aaataccagc acattcccta ccgcgaagaa gggtttgtgg acatggtcac gctgttgaaa
     7261 cagaaatggg gctttacgaa attctgggag gcctattatt acgagcgtct gaaccaggtg
     7321 gaaaagttca tgtcggatcc cgccttcgtc aaattcatcc gcacccaggg ggggacgggg
     7381 gagaagctcc tgtgggtgat caacgacgac atcttccgga aatccggcat ccggttcgat
     7441 tccaaccatc cgatcttcac ctttaacggt gtcatcggcg tgaccgaaat gttcattgac
     7501 gcggcgaaaa aattcccggg aacggaaatc aattaccaac tgctggccac ccggtattta
     7561 cgctattacg acctcttcca ccagcccgat ccggccacgg gaaagccccg tttccccaac
     7621 ctgccctaca tggaagaggg gttcatcgag agtttggtga ccggcggcta caacttcgaa
     7681 gccaacggcg gccggttcga ggacgaactg cgcatcatga agcttcggga cgtggacgcc
     7741 ctgatggggg cgaaactcct ggatcccgaa gaaaaattcc aggggatttt cgtccgggga
     7801 tacctggagg gcatcatccg atccgaatcc gcggtgcccc ccggggtcag cgccccggtc
     7861 atcgactttt tcatccttca gaaaatcgtc ggcatcggtc aaacctacaa catgctgacg
     7921 ggtccctcgg tcggcgtggg gcaaccctcc tcggtggatg ttcaaaagga ccttccggtt
     7981 ctaaagacgg ccacccgggc cctcgtgttt gtccacaacg gggtccgcca gcaaaccgcc
     8041 gataaaaaag agcgttaccc gaaacttcgg ccgatcttca acatcctcgg cgaatccgaa
     8101 aagtgggtgg ggcggatgca cgccaccggc ttgttaaaag aagaccgctt tcaggaaatc
     8161 tttacgaact acatcgatgt tctggaacgc catttcgacg agctgaaggg cacctatttg
     8221 ccgcccgagc agggcgcggg cggcgcccgt tacctgggcg aactgggaac gtacctccgg
     8281 gtcctgaaag aaaatcttcg aaaggacgcg gccgccatcg aatcgcgttt tgtgacccgg
     8341 gaccagcaaa cgcggcaatt gggccaggcc gcggggttgg aggaggaaaa ggcgggggga
     8401 accgaagcgg aaacccgcgc gcgggttgtt ttcgtccgtc ccgaaatcga cgaaagccag
     8461 ttcatcctgg acgccggcta catcgtcacc gaaatcctgc gggacatgga aagggaaatc
     8521 ggggcttcgg gcaacgccgc attgaaaaac gtgaccgtgg accggttgat gacccactat
     8581 ttcaaaacct tggcccattt aaaaaccacc cactacaaga accaaatcat gcaaatccaa
     8641 agctaccggg attcggaggc gttgggccgc ctgaacggca aaatcaacaa ctacgccatg
     8701 cggttcgcgt tcatcgacta tctggtctat tcggccgaag gggacccgaa ccttcgggcg
     8761 ttttttgaat ccaaagggct gaattccgcc cagcagttca tcgaccggtt ctacgccaac
     8821 ctcgaaatcg ttcaaaagac cgacggttta aaacaagcgc tggaagaatt gcgcaacgcc
     8881 aacgaatatt ccgcggaagg cgtggagttc agttacgcca tgatgctgac gttgaaccac
     8941 aagatcaccc ccgaaaccct taagcgcgtg gccacccaca tcccggccgt gttgcgggac
     9001 tacaacgatt atcttctccg gccgggcgcc gccgccggcc aatcgctcaa attccgggtg
     9061 gattccgcgg tcgttcttta cgaagccctg gcccgggagc tcgccctggc ccgccccttc
     9121 ggcgccaccg gcgatcgcat ggaggtgaaa caaaagcgga ccaacgagcc ctggatggtg
     9181 gacacgctgg tcaaggccgt ctacggcaac ctcttcggca tttatttgga ggacgacaat
     9241 ccggagcatc ggaagctgat gaaggaatgg gaaatcaagg ccttcggaaa agatttgaag
     9301 agcatggttc agcagttggc gagcgagccc acggtcatgg gccagaaaaa cgcctacttt
     9361 gatgaaatcg cgaaagtgga cgaggcgatt cgggagcgaa cggccaaata cgaggcggcc
     9421 aaacgcgacc tgagggaatc cttggccgac gattatcaaa tccagattcg catccttgaa
     9481 caaattcgcg ccagtctcga agagcgaatg gggtacttgt tgcagttggc cgacaccatg
     9541 gaccaaacgt acgcggaagg ggtcaaggac aaaaaccagg ccaccttgga agtgctgatt
     9601 ctgttgtccg cccttgggtt cggcgccggg ctgctggcct tttcgcggcg ccgatgggat
     9661 cgttcttccc gggagggttt gacgcgcctc cagcggcttc gcgccgattt ggccgatctg
     9721 aacgcccagc gcaagcaggt cccctacttg agcaacgcgg cgctcgcgat ctatttggga
     9781 ttgcccgcgg cctacgggct ctatttcggc gtcggcgccc cgcgcctcgc ccaggacgcg
     9841 ggttcgattt tcacccaggc gccctatcag ttcgaatcgc cccggagttc ccaagggacc
     9901 ctcttttcgg aaggcggttt cagcgttcga cagagccagg aagtgcggga cggaaagccc
     9961 gtggtggagc acaaaattct aagcgggggc tacctggtgg gccggatcga actcaacacc
    10021 ggctacgccg aatttatgcc gggcgccgtg cccggcggca acggattttc ctaccccctg
    10081 aatcccggac cgtccctggt gatgctgggt ttcggcgaaa acaagccgga cggtaaaaaa
    10141 gcctacagtt atcggggggc tctgagccaa gtcacctact cggtggattc gaacggacgt
    10201 ctcgtccttt cgggcacgct gcgggacgcg gggggcgctt tcgaggcccg gaattatcag
    10261 atggcggtga gcgtgaccgc gggccaaacc cgcgtcagcg tctccggcga tttcaccgcc
    10321 cgacgggacc tgaacgtcag cgagttcaac atcggcagcc tgaacaccca aagctacatc
    10381 ggggtgcccc agggcgcgcc gtctccgtcg gatcggtcgt tgtccttgga ggcccaaggc
    10441 acggtcgccg gcaaccgggt gtacctctat ttcggcaacg accacaagct tcccgacagc
    10501 ggcttgaccc cctggaccgt tcagtcggtc tccctcggcg gcacggccta caacaacacc
    10561 cccaacgtcc gcattgtccc cgaatcgatc acgttcaacg gccgcaaagt ggaccccgtg
    10621 gccgcctaca ccctcctgcc gaaccaattc gaccgccaca acgtggccat cgccccggtc
    10681 atcggcggtg ggacgccgct gactctgcaa accggcggga aaatcagcgt gcgtta
//
LOCUS       DAOQLS010000004        10206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_2553_length_10206_cov_4.139198, whole genome shotgun sequence.
ACCESSION   DAOQLS010000004 DAOQLS010000000
VERSION     DAOQLS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2553_length_10206_cov_4.139198"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PK362_00140"
     CDS             complement(<1..633)
                     /locus_tag="PK362_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNC73283.1"
                     /translation="MKLTILLPLTSLLFFFPSIFLADTWEDRNADRHVLLLNIDAHIE
                     LFPDFTRKSHLTVLHKVQSPEAIKNGFGEFQQDYNPKFDRLTMSAVVITPDGKRIHHN
                     KVQDKARSGDNAVYDDTHVVTLTLPRLTVGSTVELKVVKHNFNPRVPGQIELFTQIDP
                     STEAKRVRYAVTWPQGMDLTFLPLNGLQPGNSTLANGKHTREWVLEDRDEI"
     gene            807..1703
                     /locus_tag="PK362_00145"
     CDS             807..1703
                     /locus_tag="PK362_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC73284.1"
                     /translation="MRAFLAVASGGGYSRAAEAVHRTQPAVTASVKALERELGIALFE
                     RRGRRAALTPAGEALVAEAGPALTQWEGLADRLKERLSGEAQGVLRIGAGEAAVLYLL
                     PDILRGFQKKHPKVRIVLHHQRAEETLRMLKAGELDLGVRSLTIVPAWAIYRPSRDYP
                     RVAVCANGHPFAKGGTVKLSELSQHPLLFPGSQSITRVLVEEAMTKAGLAYQVGLEAG
                     GWEAIKTYAAAGFGVAVVPELCISKEDRRRLAVRSTGELFGRDSYGVVTRRGTALPPA
                     ARELIRLVDPGYSDLAPRGGKD"
     gene            3346..4116
                     /locus_tag="PK362_00150"
     CDS             3346..4116
                     /locus_tag="PK362_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73285.1"
                     /translation="MSSSGDPLIDVLRIESLQKSYGDKKAVDSVSFSVAEGEILGLLG
                     PNGAGKTTLINMALGVLEPDAGVVRVEGEDLSRRRSWALSRMNFAAVYAPLPGNLTVW
                     QNLRVFAMLYGIPRPRERIDVLLERFDLKKFRDAKCGALSSGEQTRVSLAKALLNSPR
                     LLLLDEPTASLDPATARDLRRVIKSIVREDRAGILWTSHNMREVVEVCDRVLFLSGGR
                     VLLEGDPKTLPAQHGKENLEELFIAVAQTPLALEEGAP"
     gene            4117..4914
                     /locus_tag="PK362_00155"
     CDS             4117..4914
                     /locus_tag="PK362_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC73286.1"
                     /translation="MRFHRVAAVGLRYVYLIRGSVSRVFPLFSWVAIDMVLWGFLTRY
                     LNTLSTVNFVPMLLGAVLLWDFFIRVMQGVTLVFFEDVWTRNFLNVFSTPLTVGEYVA
                     GLVLSSVVSSAIGLAVMLALAVPVFGFSFAAYGPLAVPYVMVLFLFGIAVGVLCCALV
                     LRLGPAAEWFIWPIPAFISPFVGVFYPVSTLPAWMRWVGRLLPPSYVFENLRSLVLGR
                     GPGPGGLALAGGLALLHLIVAAWVFKKVFRGAVRSGLIARYSAESVT"
     gene            5022..5198
                     /locus_tag="PK362_00160"
     CDS             5022..5198
                     /locus_tag="PK362_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73287.1"
                     /translation="MTLTELLLGAMVVVGLYYLLRPLQRALENWLLKVQGVENPRFID
                     VDRVPEKQDKPPKE"
     gene            5202..6119
                     /locus_tag="PK362_00165"
     CDS             5202..6119
                     /locus_tag="PK362_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNC73288.1"
                     /translation="MQLKRPDNFSTPEGFSWIASRGATLLVALIALVAVMNSFVIIQP
                     GYSGVIFNVWTGSLRTVGQGMAFRMPFVTKVQSYPTALRTYTMVQRAGEGSSGEDDSI
                     DLPTMEGQHIRQDISVTYNTSEDQAAQVFKAFRGADIEDIETSFIRRTFITVAQNVAG
                     QMSLTEIISSHRTQLQDEIQKKLEIELQKMGFHLDKVNLGASHLPPTIEAQMQQKMAA
                     QQEAQKAEYELQKQQTLAKAAVAQAQGEAQSILVKAKAQAEANHLLESSINAMLVQYK
                     SVEKWNGVLPEVTGGSTPFIDLRGKKSAE"
     gene            6170..6592
                     /locus_tag="PK362_00170"
     CDS             6170..6592
                     /locus_tag="PK362_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73289.1"
                     /translation="MSLPHTPPGGPPAAGPSRDIYGRMGEENIFRMCADFYRALEKTD
                     IRPLFPPDMAEASKKLGAFLVGLLGGPPLYHERHGPPRMRARHLPFPIDENARRVWFS
                     TFCGVLRNAPADYAFPPEHLPGFIAFLDAFAGWMVNRA"
     gene            6715..7335
                     /locus_tag="PK362_00175"
     CDS             6715..7335
                     /locus_tag="PK362_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73290.1"
                     /translation="MKPSAAVWMSLALLTGCAAFRIESHPEAAKAGPFRKTLVAFVGA
                     EASTRGEVEEALVRAFGVRQAAPARWSDGLATAPSPAAALATARQSGFDSLFVLEKHS
                     MVHWEGRDALPDRWEEALAPTKRESPTADLVSVESQGRAGEERFVNGRAFLFDLNTGR
                     RVWWAHGTGRADKSLRTREFVEQAARACVNALVREGLLPRRPGRDR"
     gene            7395..8717
                     /locus_tag="PK362_00180"
     CDS             7395..8717
                     /locus_tag="PK362_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNC73291.1"
                     /translation="MRGTLWFGFYLFLVVFPLILGVLGDPAAEPRPASLEAAVAAGYV
                     GLALMAFEFALISRVRSVAGAFGQDALAQFHKQIGVVALLFVMVHPALLFFNGFSPGS
                     LLNPFPEEVPGVLRWGMASFYAALSIVVLALGRKRFKIPYEWWQWSHSLLALAAVAGG
                     LFHIFGIGNYTGSAAMKSLWAVYAAVLVGLTVRYRVVRPLVRWRRPWEVVENVPERGE
                     AQTLVLRPVGHRGFAFEPGQFAWLLTGVTPFGWEQHPVSISSSAETPAGGPVSFTVKA
                     LGDWSRERVPALAPGRRLWLDGPYGVFSIDRQQGPGYVMIGGGVGITPLYSMCLTMAD
                     REDGRPVTLFYGSRRWEDVTFREGWESLSRRMNLKIVHVLEEAPAGWTGETGFITAAI
                     LQKQLPKGFRRFPFFVCGPPPLMDAMEDVLPGLGVDEDRIHTERFDMV"
     gene            complement(8993..9292)
                     /locus_tag="PK362_00185"
     CDS             complement(8993..9292)
                     /locus_tag="PK362_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73292.1"
                     /translation="MADADLSPVVPPTARWVKLQFQLKGKTPGARLTARVWSGAPEGA
                     VVLNGPEGFAFVRLAAPQRLSYQAPAEVELKLKVVAFKDGGEPPAGPANNDSSRG"
     gene            complement(9298..9735)
                     /locus_tag="PK362_00190"
     CDS             complement(9298..9735)
                     /locus_tag="PK362_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73293.1"
                     /translation="MLPRRHRREPTGPVPGFTLIELMIVVAIIGLLAAIAIPKFANLT
                     YKAREATVKNKLSAFRSAINLYYADTEGLYPQDDTALVPRYLAEIPRIGLPAPINHLD
                     TNDVGNLVNDWATNRAWFYDGSNGRLYVNCTHTDTRGVTWSLY"
     gene            9976..>10206
                     /locus_tag="PK362_00195"
     CDS             9976..>10206
                     /locus_tag="PK362_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNC73294.1"
                     /translation="MTQFDATLKPETLALHGGQEADPTTGSRAVPIYQTTSYQFKSSD
                     HAANLFGLREFGNIYTRLMNPTTDVLEKRIAAL"
BASE COUNT         1726 a         3047 c         3388 g         2045 t
ORIGIN      
        1 gatctcatcc cggtcttcga ggacccattc ccgcgtgtgt ttgccattgg cgagggtaga
       61 atttccaggt tgaagcccgt tcaacggcaa gaaagtgagg tccatcccct gaggccaggt
      121 gacggcgtag cgtaccctct ttgcttcggt cgatgggtcg atctgggtga ataactcgat
      181 ttgaccgggg acgcggggat tgaagttgtg tttgacaacc tttagctcga cagtggaccc
      241 caccgtcagt cggggcagtg tgagggtgac cacgtgggtg tcgtcataga cggcattatc
      301 ccccgagcgg gctttgtctt ggactttatt gtggtggatg cgtttcccgt ccggggtgat
      361 gaccacagcg gacatcgtta gcctatcgaa ctttgggttg tagtcctgtt ggaactcccc
      421 gaacccgttt ttgatcgctt cggggctctg caccttgtgg agaaccgtga gatgggactt
      481 tcgagtgaag tccggaaaga gttcgatgtg cgcgtcgatg ttcagcagga ggacatgccg
      541 gtcggcgttg cggtcctccc aggtgtcggc gaggaagata gacgggaaga aaaagagcag
      601 gctggtgagg gggagaagga tcgtaagttt catatggcta ccgcctttcg gaccgaagtc
      661 cggtgagatc gagcagcccc tggtgtcgga gagcgggtgg gctcgcctct cactcccatt
      721 gtagggtggg agaataaata aatcaaactg ataatattga tatctgatat aaatcctgtt
      781 aaactattgt tatggaaatc caacagatgc gggcgttctt ggcggtggca agcgggggcg
      841 ggtattcccg cgcggcggag gcggtgcacc ggacgcaacc ggcggtgacg gcgtcggtaa
      901 aagcgctgga gcgggagctg ggtattgccc tgttcgagcg gcgcggccgt cgggcggcgc
      961 tgaccccggc cggcgaggct ctggtggccg aggcgggccc ggccctgacc cagtgggagg
     1021 gcctggcgga caggttaaaa gagcggctga gcggagaggc ccagggggtg ctgcggatcg
     1081 gcgcggggga ggcggcggtg ctgtatttgc tgcccgacat tttgaggggg tttcaaaaga
     1141 aacatccgaa ggttcgaatt gttctccatc accagagggc ggaggaaacg cttcgcatgt
     1201 tgaaggcggg ggaactggac ctgggcgtca ggtcgctgac catcgtcccc gcttgggcga
     1261 tctaccgtcc gtcgcgggac tatccccggg tcgcggtgtg cgccaatggg catcccttcg
     1321 ccaagggcgg gacggtcaaa ctgtcggaac tgtctcagca tcctctttta tttcccggtt
     1381 cccagtcgat cacccgcgtt ttggtggaag aggccatgac caaggcgggg ttggcctacc
     1441 aagtgggtct ggaggccgga ggttgggagg cgatcaagac ctacgccgcc gccgggttcg
     1501 gcgtggcggt ggtgccggag ctgtgtatat cgaaggaaga ccgtcgacgg cttgctgtgc
     1561 ggagcacggg agagttgttt gggcgtgaca gttacggtgt ggtcacccgg cggggaacgg
     1621 cgttgccccc agccgcccgg gagctcattc ggttggtgga cccggggtat tccgacttgg
     1681 cgcccagggg aggcaaggat tagctggacc tataggaaag ttcagccgtc tggggagggt
     1741 tgggaggaag tttgatccca gcccttttta tttttttggg agagcttttc ggcggcggct
     1801 atggctgttg gatgccgccg ccgggatatg ggtggggaag gaggggcatg ggcgggattt
     1861 aaaggaggag taaaccactc cgcggcggtg gccgcgtggt ttttggtggt gctctccggg
     1921 gggtgtgggg ggccaccagc caggcagccc cccataaacc ccccgagccg ggcccgctgt
     1981 tggccgggat tggggggagg gggggcgggg gttgcctggc agcgccgcca gcggtgccgg
     2041 gcgggccgtt gtgtggcctg cacggcggcg caatggggcg gcgcttgcgg aggccaccga
     2101 aaagcacaga tcgtttgtga cccctgggag cgaaaccttt tcaggagaca ggcggggcgg
     2161 tggcgaagta tgccgccgcg gcttgggaag ggagcgggcc cacgcgcgag gcgcgacggc
     2221 tgagtgtgcc gtgccttgga aacaaggctg ggcccgtgac tgaaggtagg ccccggggac
     2281 ttcggataca tatatttgac cccggggacg aaaatacatg actgttgccg aagtggggca
     2341 acttaccttg aagtgcgcgc gccctacggg gtgaaaagct tttgacgtgc cgagcaacgg
     2401 agctgaaaac tcctccacag ccctggggag tggccacgcg gacgggcggc cgtgaccggg
     2461 gaaaccgaag ccaaccgact ccaccaagcc cgtgggcggg gccgcgggcc gcgctccgga
     2521 gcggttctgg aacgattcaa ccggagcgga cagcgttaca gtcgcgcggg cgaccgagcg
     2581 gagctgcggc ggcgcgggct cctcgtgccg acgcggcgga gcgacccgaa agacgacccg
     2641 accggagcga gcggcggggg tttttctccc cgggcagcga atcaaccgga gcggatttgg
     2701 aacggattga ccgaagcaga atgcggtccg gtcgcgggga agacgaagcg agctggaccc
     2761 gcgcgagctt ttcgggcggg gccggcgagc gaccaagggg gcggacgatg gggcggtgcg
     2821 gcaagcgccg gggtttttcc cggcgggcgg gtttggagtt ttcggggacg acagccatgg
     2881 cccgggtttt cggaactgag gcggcggccc cgcgccgggg gctatgtccc tctgtccccc
     2941 gtctctctgt tccgcacagg acaaatagag tggccccgtg tggcgccgcg atagcggtcg
     3001 acggtcaaaa aacgccccct cacccggctt tcagccaccc tctccccaga ggggcgaggg
     3061 aactagaaac caggcctttt agccggggga aggtaaaagt ccccggtcat tcgaaagttt
     3121 tcccaatagg catgtgctat atttgggaag tcgaagggga cgacaatttt gaagttttta
     3181 aggaagtccc cagtttcatc ccgtttgttt cagggcgcag cgttgttcgg cccggacacc
     3241 cacgcacgat gtcaatcggt atcgtcccgg tttgtcggac gggagagctt tgggtgcggc
     3301 cgttactcac ccgcgttttg tcgttcccgg cactggccgg taagcgtgag ttcttccgga
     3361 gaccctctta tcgacgtcct gcgcatcgaa tccctgcaaa aaagctacgg cgacaaaaaa
     3421 gcggtggatt cggtttcctt ttccgtcgcc gagggggaaa ttctgggcct gttgggtccc
     3481 aacggcgccg gaaagacgac gttgatcaac atggccctgg gcgttctgga accggacgcg
     3541 ggcgtcgttc gggtggaggg ggaggatttg tcccggcggc gaagctgggc tctgtcccgc
     3601 atgaactttg ccgcggttta cgcgccgctc cccgggaatt tgaccgtttg gcaaaacctg
     3661 cgggtcttcg cgatgcttta cgggattccc cgaccccggg aacgcatcga cgtccttctg
     3721 gaacgatttg atttgaaaaa attcagggac gccaagtgcg gggccctctc ctccggcgaa
     3781 cagacccggg tgtccctggc caaggccctg ctgaacagcc cccgacttct cctcctggac
     3841 gaaccgacgg cgtcgctgga cccggcgacg gcccgggacc ttcgacgggt gattaaatcc
     3901 atcgtccggg aggaccgggc cggcatcctt tggacctccc acaacatgcg ggaagtggtc
     3961 gaggtctgcg atcgggttct ctttctctcg gggggccggg tgcttctgga gggggatccc
     4021 aaaaccctgc ccgctcagca cgggaaagaa aacctcgaag agctctttat cgccgttgcc
     4081 caaacccccc tggccctgga agaaggggcc ccgtaagtgc gtttccaccg cgtcgccgcc
     4141 gtcggcctcc gctacgtcta cctgatccgc ggcagcgtgt cccgggtctt tccgctgttt
     4201 tcctgggtgg ccatcgacat ggtcctctgg gggtttttga cgcggtacct caacaccctg
     4261 tcgacggtaa atttcgttcc gatgcttctc ggggccgtgc tcctctggga ttttttcatc
     4321 cgggtcatgc agggggtcac cctggttttt tttgaagacg tttggacccg gaactttctc
     4381 aatgtttttt cgacccccct caccgtgggg gaatacgtgg cggggttggt gctttccagc
     4441 gtggtgtcca gcgccatcgg cctggcggtc atgctggccc tggccgtgcc ggtcttcggc
     4501 ttctcctttg cggcctacgg ccccctggcg gttccctacg taatggtcct ctttctcttc
     4561 ggcatcgccg tcggcgttct ctgttgcgcc ctggtgttgc gcctggggcc tgcggcggag
     4621 tggttcatct ggcccattcc ggcgttcatt tcgcccttcg tcggcgtttt ctaccccgtg
     4681 tccacccttc cggcctggat gcgctgggtg gggcgtctcc tgccgccctc ctacgtcttt
     4741 gaaaatcttc ggtcgttggt cctggggcgt gggcccgggc ccggcggttt ggccctggcc
     4801 gggggactgg ctcttttgca tttgatcgtg gccgcttggg tattcaaaaa agttttccgc
     4861 ggcgccgttc gcagcggact catcgcgcgt tacagcgccg aaagcgtgac ctgaaacccg
     4921 gaggccgcgc gccggagatt cttaaacttg taaagtatag gctcggcttc aaagcggggc
     4981 ttcccctccc cgaattgaac tcttacacga ggcggaatcc tatgacgtta accgaacttc
     5041 tgctgggcgc gatggtggtc gtgggccttt attaccttct tcggcccctg cagcgcgcgc
     5101 tggaaaattg gttgttgaag gtgcagggcg tcgaaaatcc ccggttcatc gatgtggatc
     5161 gcgtccccga aaagcaagac aaacccccca aggagtgaca cgtgcaatta aaacgacccg
     5221 ataatttttc cacccccgaa ggcttttcct ggatcgcgag ccgcggggcc acgctcctgg
     5281 tggctcttat cgccctggtg gcggtcatga actcctttgt gatcattcaa cccggttatt
     5341 cgggcgtgat cttcaacgtt tggaccggtt cccttcgcac cgtggggcag ggcatggctt
     5401 tccgcatgcc cttcgtgacc aaggtgcaga gctaccccac ggcccttcgg acctacacca
     5461 tggtgcagcg cgcgggggaa ggctcctccg gcgaggacga cagcatcgac ctgcccacca
     5521 tggagggcca gcacatccgg caagacatct cggtgacgta caacacttcg gaggaccagg
     5581 ccgcccaggt gttcaaggcc ttccgcggcg cggacatcga ggacatcgag acgtccttca
     5641 ttcggcggac cttcatcacc gtggcccaaa acgtggcggg ccagatgtcc ctgaccgaaa
     5701 tcatctcctc ccatcggacc caattgcagg acgaaatcca gaaaaaattg gagatcgaac
     5761 ttcaaaaaat gggtttccat ctggacaagg tcaatctggg ggccagccac ctgccgccga
     5821 ccatcgaggc ccaaatgcag cagaaaatgg cggcccagca ggaagcccag aaagccgaat
     5881 acgaattgca gaagcagcaa accctcgcca aggccgccgt ggcccaggcc cagggcgaag
     5941 cccaatccat tctcgtcaaa gccaaggccc aggccgaagc gaaccatctt ctggaatctt
     6001 ccatcaacgc gatgctcgtc caatacaaat ccgtggaaaa atggaacggt gttttgcccg
     6061 aggtgaccgg cgggtccacg cccttcatcg acttgcgcgg caagaaatcg gcggagtaaa
     6121 tcccgcccca atccgttgac ggagagattc cgttgaaggt ccgcccccgg tgagccttcc
     6181 ccacacgccc ccgggcggac cgccggccgc gggcccctcc cgcgacatct atgggcgaat
     6241 gggcgaggaa aatattttcc ggatgtgcgc ggatttttac cgcgccctgg aaaaaaccga
     6301 cattcgcccc ctgtttccgc ccgacatggc cgaggcctcc aaaaaattgg gggctttcct
     6361 cgtgggtctc ctgggcggcc cgccgttgta ccacgagcgc cacggccccc cgcgcatgcg
     6421 ggcgcgccat ctgccgtttc ccatcgacga aaacgcccgc cgggtgtggt tctccacctt
     6481 ttgcggcgtc ctccggaacg cgcccgccga ctacgccttt ccgccggaac acctccccgg
     6541 cttcatcgcc ttcctggacg ccttcgccgg gtggatggtc aatcgcgcct aaggggtccg
     6601 gggggcgctg cccgccgtca tcgacaatcc tgattgtcgt catagcgttt tccgccccgg
     6661 cctggtaagg taagggcgag cccgccccgt agcggtcggg ccggaggaat ccccatgaaa
     6721 ccgtccgcgg ccgtctggat gtccctggcg ttgttaacgg gatgcgccgc ttttcggatt
     6781 gaatcccacc ccgaagcggc caaagcggga cccttccgga aaaccctcgt ggccttcgtg
     6841 ggcgccgagg cgtcgacccg gggggaggtg gaggaagccc tcgtgcgcgc cttcggcgtg
     6901 cgccaggccg cgcccgcccg gtggtccgac gggttggcca cggccccttc gcccgccgcg
     6961 gccctggcga cggcccgcca atcggggttc gattccctgt tcgtcctgga aaagcattcc
     7021 atggtccatt gggaaggccg cgacgcattg cccgatcgat gggaagaggc gctggcgccg
     7081 accaaacggg aatcccccac ggccgatctc gtgtccgtgg aaagccaggg ccgggcgggg
     7141 gaggagcgtt tcgtgaacgg ccgggctttc ttgtttgatt tgaacaccgg ccgacgggtc
     7201 tggtgggccc acggcacggg acgtgccgac aaaagcctgc gcacccggga atttgtcgaa
     7261 caggcggccc gcgcctgcgt caacgccctg gtccgggagg ggttgttgcc ccgtcgaccg
     7321 ggacgggatc ggtaggccgg gcggtcggcc cgggcgaagc agatccacga aggagaaatc
     7381 caacgaatcg cgtcgtgcgc gggaccctgt ggtttgggtt ctatcttttt ctcgtggtgt
     7441 tcccgttgat cctcggcgtt ttgggggacc cggcggcgga gccgcgcccc gcgtccctcg
     7501 aagccgcggt ggcggcgggc tacgtggggt tggccctcat ggccttcgaa ttcgcgctca
     7561 tctcccgggt gcggtccgtg gccggggcct tcgggcagga cgccttggcg caatttcaca
     7621 aacagatcgg ggtcgtcgcc ctgctgttcg tgatggttca tcccgcgctt ctctttttca
     7681 acggattttc gcccgggtcg cttttgaacc ccttccccga ggaggttccc ggcgtccttc
     7741 ggtgggggat ggcctctttt tacgcggcgc tgtccatcgt cgttttggcc ctcggccgca
     7801 agcggtttaa gatcccctac gagtggtggc agtggagcca ctctttgctg gccctggcgg
     7861 ccgtggcggg tgggttgttt catattttcg ggatcgggaa ttacaccgga tcggcggcca
     7921 tgaaaagcct ttgggcggtt tacgccgcgg ttctggtggg actcaccgtg cgttaccggg
     7981 tggttcggcc cctcgttcga tggcgacggc cttgggaagt ggttgaaaat gttcccgaac
     8041 ggggagaagc ccaaaccctg gtcctccgac ccgtcggcca ccggggtttt gccttcgaac
     8101 cggggcaatt cgcctggctg ctcaccggcg tcaccccctt cggttgggaa cagcatccgg
     8161 tttccatttc ctcctccgcc gaaacccccg cggggggccc cgtgtctttc accgttaaag
     8221 ccctgggcga ttggtcccgg gaacgcgtgc ccgccttggc ccccggtcgc cgcctttggt
     8281 tggacggacc ctacggggtc ttttccatcg accgccagca ggggccgggg tacgtgatga
     8341 tcggcggcgg cgtgggcatc accccccttt attcgatgtg cctgaccatg gccgaccggg
     8401 aagacgggcg tccggtcacc ctgttctacg gcagtcggcg gtgggaggac gtgaccttcc
     8461 gggagggatg ggaatccctg tcccggcgaa tgaatttaaa aattgtccac gtgctggagg
     8521 aggcccccgc ggggtggacc ggcgaaacgg ggttcatcac ggcggcgatt ttgcaaaaac
     8581 agttgccgaa gggttttcgg cgcttcccgt ttttcgtctg cggtccgccc ccgctcatgg
     8641 acgccatgga agacgttctg cccggcctgg gggtcgacga ggaccgcatc cacaccgaac
     8701 gatttgacat ggtttaagag cctatcccaa taggtcattc ggggctgcaa acgcggagga
     8761 ccggccatcg tcttcgggcg gtcccggcca cgtgctcgcc gagcacgcct gccgggccct
     8821 tcctgcgccg ctgtccactc ctccacgttt tcgctcccaa agcacctatt gggataggct
     8881 ctaaggagag cgacgtgcga catcgcatcg tgacgcgggt ggtggtgttt gtggcggtcg
     8941 tgttggtggc ggcctgcgtg ttgttcgtcc tgggagtgac cccccggggc ggttagccgc
     9001 gggaagagtc gttgttcgcg ggtccggcgg ggggctcccc gccgtctttg aaggcgacca
     9061 ctttcagttt caattccacc tcggcggggg cctggtagga aagacgttgg ggcgccgcaa
     9121 gccggacgaa agcgaatccc tccggaccgt tcaggaccac ggccccctcc ggcgccccgg
     9181 accacacccg ggccgtcagg cgcgccccgg gcgtcttgcc tttcagttga aattgcaatt
     9241 tgacccaccg ggcggtgggc ggcaccaccg gggacagatc cgcgtcggcc atcgtcgtca
     9301 gtagagggac caggtgaccc cgcgcgtgtc cgtgtgggtg caattgacgt agaggcgtcc
     9361 gttggaccca tcgtaaaacc aggcgcggtt ggtggcccag tcgttcacga ggtttcccac
     9421 gtcgttggtg tccagatggt tgatgggcgc cggcagaccg atgcggggga tttcggccag
     9481 ataccgcggt acgagggccg tgtcgtcttg agggtacagc ccctccgtgt cggcgtagta
     9541 aagattgatg gccgagcgga aggccgacag cttgtttttc accgtggcct cgcgggcctt
     9601 gtatgtcagg ttcgcgaatt tgggaatggc gatggcggcc aaaagaccga tgatggccac
     9661 gacgatcatc agctcgatca gcgtgaagcc gggaacgggg cccgtcggtt ctcgtcggtg
     9721 tcgtcggggg agcacggggc cattcaatca aaataccgag gggaacgaaa gggtcttctt
     9781 ggacttcccg ggcgattttt gtatagtttc ggaaacggtg aggctttaat ttccaacttg
     9841 ccccagccgt gcggcgcgcc aagcgcgacg cgaattgggc gctaaagcgg tggagtccct
     9901 caatttttcg acggcccgca ccgcaacact cggcgcgggc ttttttcttc ccgccccaca
     9961 aaacggagca ttcccatgac gcaattcgac gccacgttaa aacccgaaac cctcgcgctt
    10021 cacggcgggc aggaggccga tcccaccacc gggtcccggg ccgtgcccat ctaccaaacg
    10081 acgtcctatc agtttaagag ctcggaccac gcggccaacc ttttcggcct gcgggagttc
    10141 ggcaacatct acacccgatt gatgaacccc accaccgacg ttctggaaaa acgcatcgcc
    10201 gccctg
//
LOCUS       DAOQLS010000005         9869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_2680_length_9869_cov_4.427247, whole genome shotgun sequence.
ACCESSION   DAOQLS010000005 DAOQLS010000000
VERSION     DAOQLS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2680_length_9869_cov_4.427247"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1174)
                     /locus_tag="PK362_00200"
     CDS             complement(149..1174)
                     /locus_tag="PK362_00200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNC73295.1"
                     /translation="MTPRSVLVVRLSSLGDVVLTAPVFDNLRRAWPEARLTLLVKKAF
                     APALAGHPAVHELLMFEDLGVWGALREIRRRRFDVLVDLHDTLRSRIWAMFSGAGRVV
                     RYRKRAWARRLLVCFKRPSSDLAGKTVDRYLETLAVLGVDVRDRAPRLPAPADGLPAA
                     AAALGAGPWIVLSPGALHATKRWPAERFAAAADRLAEGFRSAGGAAPTVLLVGSPSDR
                     PSAEAVLETLQGPALNLTGRTSLKELMALLSRAAVLLCNDSGAMHLGAALGVPTVAVF
                     GPTVEAFGFFPSGEKTAVVQNAGLACRPCSLHGGRRCPKKHFRCMTDVTVDRVVAEAR
                     RLLGAAS"
     gene            complement(1272..2570)
                     /locus_tag="PK362_00205"
     CDS             complement(1272..2570)
                     /locus_tag="PK362_00205"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNC73296.1"
                     /translation="MLTLYSFLTALLSPLILLVFVWRYGLRRTLRGLPERFVLGGSDG
                     SPKPTLWIHAASVGEVRAAESFLRAAPARYPGWPRLLTTTTVNAKELAERLDVAEFVR
                     LAPVDRPGPLGRFVRRWNPRALILVETELWPQWLKGLSENNVPVCVINGRMSDGAFPW
                     YYRLRSFWEPLLATLARAGVQSPTNASRFLQLGALPDAVAITGNLKFDVPLPDPARRA
                     ALRALYRVEGDAPVWVAGSTHDGEEEIVLGAFLRLRREGVPLRLVLAPRHVERAGEVR
                     RYAENTGFRAVLRTRLSEGDAPWDLLVLDTVGELADVYGLATVAYVGGSLIARGGQNP
                     LEPARWGVPVLFGPHMTNFREMAKLFEERRAALRVADAESLLAALRDLIASPEKRAAL
                     GDAARACADAQRGALEANLALVAEGLASGPRRPRGACGGC"
     gene            complement(2564..3235)
                     /locus_tag="PK362_00210"
     CDS             complement(2564..3235)
                     /locus_tag="PK362_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC73297.1"
                     /translation="MTQALSDSRSRRDRFFPWVVSWLGWLILQFIGRTSRVAVHWSPA
                     AQPLRDDGRPLIFAFWHRHQLLMAWEHRRRGVTVLVSRSRDGELIAGVLERLGYRTAR
                     GSSSRGGPAAFMELLDAVAAGGRVAFTPDGPRGPAGSVQPGVLALAAKTGVPIVPVAW
                     AGTRVKTLSSWDRFMIPLPFGRFEVVFGNPLPVSAVDSAAEQNLRRALEGAEAEAVRR
                     LEAPC"
     gene            complement(3380..3988)
                     /locus_tag="PK362_00215"
     CDS             complement(3380..3988)
                     /locus_tag="PK362_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73298.1"
                     /translation="MAETVFSVKKIKKFRLQPRAQAVMRNLRALSENAQATPELEKAV
                     ETEIRSVASSLETAALYATFSPDACPTGLASVRPGEGERPPLSLTLFAATVGVGLEEE
                     IARALARGEALRSRVLTALGEELADQAGQFVARLINEEAKDDAFELSDRRPLTSLDEQ
                     KTLLDTLDAARIPLSFDAGGHLTPRFSRLGYVLWWPPSKKRS"
     gene            complement(3988..4944)
                     /gene="secF"
                     /locus_tag="PK362_00220"
     CDS             complement(3988..4944)
                     /gene="secF"
                     /locus_tag="PK362_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNC73299.1"
                     /translation="MEFFKTPNFDYIKYHKLFFALSGLLAAATVVGVVVRGPSLMSID
                     FTGGTLVQGYFQKAGEPAAGAGGQNVEISLDRVRAALATAGLAGAELQSVPNHNAIIL
                     RFKSGAGEAKTELAGKVTEAFARAFEGFSFTTERVEFVGPVVGKQMVSKAILAVFFSM
                     LGIVVYVAFRFRNWIWGVSGVFALVHDVFLALGFMALTGREISITVVAALLTLAGYSI
                     NDTIVIFDRIRENIRLRGRHSKEPLDVLINRSCNETLSRTIITSLTVFIVLLSLLFLG
                     GEVIRDFSLALTFGVIVGSYSTIFIATPMVYLWQRKRNKPLH"
     gene            complement(4944..6383)
                     /gene="secD"
                     /locus_tag="PK362_00225"
     CDS             complement(4944..6383)
                     /gene="secD"
                     /locus_tag="PK362_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNC73300.1"
                     /translation="MPKAYWKSLAILATVLLSAWYLFPSYSFYRLSREKQQELRDRRN
                     PILGRILNLGLDLQGGIHLLLELQTDKLPDDTPETLREAIDRAMEVVRNRIDQYGLSE
                     PLIVRQGDKYIVVQLPGVKDRNQAKDLIGRTALLEFRLLESDVVPEAVLNKAREMGLT
                     AAQLRPGKMPKEIAELLPAGTDLLAGKESAFYLVKASAALTGAGLANARVEMGAHEFS
                     GASPHVSLEFNPEGAKRFADLTEANVGRRLAIVLDGVVQSAPSIRSRIPDGHAIIEGN
                     FSPEEAKFLKSVLQAGALPAPLEIVEERTVGATLGDDSVRAGVLAGAIGLGAIFLFMV
                     IYYRWSGLLANLALVLNLVLLLAAMAALHATLTMPGIAGIILTIGMAVDANVLILERM
                     REEVRLGKSPRMVVDQGYDKAFSAIFDGNLTTIIAAVFMFQFGTGPVKGFGVSLMLGL
                     AVSMFTAIVVTHTVYDLWLMHRDVQKLSV"
     gene            complement(6376..6765)
                     /gene="yajC"
                     /locus_tag="PK362_00230"
     CDS             complement(6376..6765)
                     /gene="yajC"
                     /locus_tag="PK362_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNC73301.1"
                     /translation="MKRFSSLLLLLSAAAPVWADGRPAGAPNGGLMMMVPWIAILGVF
                     YFLLIRPQQKQAKQHQALLDNLKRGDRVLTQGGLYGTIQAVKGKVLEVKLADDVKVLL
                     ARSHVSQVVSNDPAQETEPTATNGAHA"
     gene            complement(6762..7928)
                     /gene="tgt"
                     /locus_tag="PK362_00235"
     CDS             complement(6762..7928)
                     /gene="tgt"
                     /locus_tag="PK362_00235"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNC73302.1"
                     /translation="MTERAPGHFELETNDGAARAGRLTLAHATVETPIFMPVGTQATV
                     KTLSADDLETLGYSFILANTYHLWLRPGIDLLEKSGGLHAFMSWKGAILTDSGGFQVF
                     SLANRRDVSEEGVSFSSHVDGARHRLTPETVVDAQLRMGVDVAMCLDECPPYPVAEAD
                     AREMMERTLRWTGRARERWAADDRRGKTLLFPIVQGATFPALRTESARRTAALDFPGY
                     AVGGLSVGEPRALLGEMIGASVRELPAAKPRYLMGVGRPEDVLTAVERGIDMFDCVWP
                     TRNARNGQVMTWDGSYNVRVAALREDGRPLEPSCACPVCRRYTRRYLAHLFRAGETTA
                     LRLLTLHNLAFMLDFLRQIRRAIVRREFTEFKHAFLERQSRADGPPAGISQGVL"
     gene            complement(7962..>9869)
                     /locus_tag="PK362_00240"
     CDS             complement(7962..>9869)
                     /locus_tag="PK362_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73303.1"
                     /translation="HQKVYNALVPYQGTINTIVFRRDGDRLNALEKRFDALVADFAAQ
                     SPNPMDVESVRALVDGVRSDLARAREFLNYPLETWQIRVHALLDRYNNTTEENFPEPV
                     LWDQELAAIQSVAAELRADLEKSPFPDMMERHAEMEVEALGLIEDHLAEERREYRETL
                     GGRWPLLETIYRRIGGLGGLVLLPVTLESVVPALLSLSVGAAVGISGGVPLWGFAAYQ
                     MAWHLAHVPGAPGRGADRLLSAFSQEKMAITLGVFFGILGAQALGLADAGGLSFWASL
                     AALALPHAAVNAGLWLATRRALRPAVEIPIAKGVNVRPETDTQRLLNGLLFGGPAESR
                     IAYSRFVVSRTEAVRRALVERAAARAQRERLAAILGLAPGSSMGDIAFRLGRVHRGGG
                     DLASIVRGRGWPGARLDARQAHDVAALISLAGGWSLIPNLPKKALKVMREYHRGEGLV
                     DFHERIVQSLTDRGAGETPRVVLFEITTNLLDGKTKLEGRDEAIRRALEFWARRPNTV
                     VNRVVWVLPQGRSAVDLIALFRRTPALAPLLDGGLKGIPIVSPPTQKGRLKSITALLL
                     RAQNLSNPGELRPADLYLLNRSEWDLSDLPGHLKGLVNFLILLAGDVVFDATDRLADD
                     VKRLNVVSTNA"
BASE COUNT         1936 a         3158 c         3287 g         1488 t
ORIGIN      
        1 ccctccgccc gggcggcccg atcggccggg ccgtccgggt gggcggcgat ccacacggcg
       61 tgtccccggt cccgcagggc ccgggccgcg ccgaggccgt aggcggtcaa accggaatcc
      121 caccgctcgt cgaggacgtg caaaatgttc acgacgccgc cccgaggagg cgccgggctt
      181 ccgccaccac ccggtccacc gtcacgtcgg tcatgcagcg aaagtgtttt ttcgggcatc
      241 ggcgtccgcc gtggagcgaa caggggcggc aggccagccc cgcgttttga accacggcgg
      301 ttttttcccc cgacgggaag aagccgaagg cctcgacggt ggggccgaac acggccacgg
      361 tgggaacgcc cagggccgcc cccagatgca tggcccccga atcgttgcaa aggagcaccg
      421 ccgcgcggga caaaagggcc atcaactcct tgagcgaggt ccgcccggtc aaattcaggg
      481 cgggcccttg aagggtttcg agcacggcct ccgccgacgg ccggtccgac ggggatccca
      541 cgaggagcac cgtgggcgcg gccccccccg cgcttcgaaa accctcggcc agtcggtccg
      601 ccgccgcggc aaaacgttcc gcgggccacc gcttggtggc gtgcagggcc ccgggcgaca
      661 ggacgatcca gggtcccgcg cccaatgccg ccgccgcggc cggaagccca tcggcgggcg
      721 cgggcaaacg gggcgcccgg tcccgaacgt cgacccccag aaccgccagg gtctccagat
      781 agcggtccac ggtctttccc gccaaatcgc tcgatggccg tttgaaacag accaaaaggc
      841 gccgggccca ggcgcgtttc cggtaacgga cgacccgccc ggctccggaa aacatggccc
      901 agatccggga ccgaagggtg tcgtgcaagt cgaccaggac gtcgaaccgc cgacggcgga
      961 tttcccgaag agcgccccaa acgccgaggt cctcgaacat caacagttcg tggaccgcgg
     1021 gatggcccgc caaggcggga gcgaaggctt ttttgacgag gagagtgagg cgcgcctccg
     1081 gccaggcgcg gcgaagattg tcgaacacgg gggcggtgag gaccacgtcc cccagggagg
     1141 acaaccggac gaccaggacc gaccgggggg tcaagggagg acctcgccca aaaacgctcg
     1201 gtccccattc gcaacgggct tcgacccgtg cgaatacggg agggtcttcc tatccaggcg
     1261 gtccctccgc gtcagcatcc gccgcatgcg ccccgggggc ggcggggacc cgacgccagg
     1321 ccttccgcga ccaaggccag gttggcctcc agggctccgc gttgggcgtc ggcgcaggcc
     1381 cgggccgcgt cgcccagggc cgcgcgcttc tcgggcgaag cgatcaaatc ccggagggcc
     1441 gcgagcaggg attccgcgtc ggccacccgg agggccgcgc gacgctcttc aaaaagtttc
     1501 gccatctccc ggaaattcgt catgtggggg ccgaaaagga ccggaacccc ccaccgggcg
     1561 ggctccaggg ggttctgacc gccccgcgcg atcaaactgc cgccgacgta ggccacggtg
     1621 gcgaggccgt agacgtcggc caattcgccg acggtatcga gaaccaggag gtcccagggc
     1681 gcgtcgccct cggacaaacg ggtccgaagg acggcccgaa atccggtgtt ctcggcgtag
     1741 cggcggacct ccccggcccg ttccacgtgg cggggggcga gaacgaggcg caggggaacg
     1801 ccctcccgac gaaggcgcag gaaggccccg agaacgattt cctcctcgcc gtcgtgggtg
     1861 ctgcccgcca cccacaccgg ggcgtcgccc tcgacccggt aaagggcccg cagggcggcg
     1921 cgccgcgccg ggtccggcaa agggacatcg aatttgagat ttcccgtgat ggcgacggcg
     1981 tccggaaggg cgcccaactg gagaaagcgg gaggcgttgg tggggctttg aacgcccgcc
     2041 cgcgcgaggg tggccaacaa cggctcccaa aaagagcgca ggcggtagta ccaggggaac
     2101 gctccgtccg acatccggcc gttgatcacg cagaccggga cgttgttttc cgaaaggcct
     2161 ttcagccact ggggccacag ttcggtttcc accaggatca acgcgcgggg attccatcga
     2221 cggacaaaac gccccagagg cccggggcgg tccaccgggg ccagccggac gaattccgcc
     2281 acgtccaaac gctccgccaa ttccttggcg ttcaccgtgg tggtcgtgag caggcggggc
     2341 cacccgggat atcgggccgg cgcggcccgc agaaatgact ccgccgcccg gacttccccc
     2401 accgaggcgg cgtggatcca gagcgtcggt tttggcgatc cgtccgagcc gcccaggacg
     2461 aaccgctcgg ggagaccccg cagagtgcgc cgcaacccgt agcgccatac aaaaacgagg
     2521 aggatcaggg gcgacagaag ggcggtcagg aaggagtaca acgtcagcac ggcgcctcca
     2581 atcgacgaac cgcctcggcc tcggcgcctt cgagcgcccg gcgcaggttt tgttccgccg
     2641 ccgagtccac ggccgacacg gggagcgggt tcccgaagac cacttcaaac cggccgaaag
     2701 gcagaggaat cataaatcgg tcccagctgg agagcgtttt gacccgggtc ccggcccaag
     2761 ccacgggcac aatgggaacg ccggttttgg ccgccagggc cagaacgccc ggctgaacgg
     2821 acccggccgg cccccggggt ccgtcggggg tgaaggcgac ccgtccgccc gcggccaccg
     2881 cgtccagcaa ttccatgaag gccgcgggtc ccccccggct ggaggatccc cgggccgttc
     2941 gatagcccaa acgctccaga acgccggcga tcaactcccc gtcccgactg cggctgacga
     3001 gcacggtcac gccgcgccgc cgatgctccc aagccatcag caattgatgc cgatgccaaa
     3061 aggcgaagat caagggccgc ccgtcgtccc gaaggggttg ggcggcgggg gaccaatgga
     3121 ccgccacgcg ggaagttcgt ccgatgaatt gaagaatcaa ccaccccaac caggagacga
     3181 cccagggaaa gaaccggtcg cggcgggagc gggaatcgct tagggcctga gtcaaaataa
     3241 attgagcgtt ttgccgggag gattttggcc cagggcaagg cgcgacgagg gaacataccg
     3301 gtcgtatgcg accgaggagc aacacggcac ggggccaaaa gcatccggca aaatgttcaa
     3361 ggtatttcga ctccggccct caggagcgtt ttttggaggg cggccaccac agcacgtacc
     3421 ccaggcggga aaaccggggc gtcaggtgcc cgccggcatc gaaggagaga gggattcggg
     3481 ccgcgtcgag ggtgtccaaa agggtttttt gctcgtccaa ggacgtcagc ggacgtcgat
     3541 cggaaagttc aaaagcgtcg tccttcgctt cttcattgat gagacgggcc acgaactgac
     3601 cggcttgatc ggccaactcc tcccccaagg cggtcagcac gcggcttcgg agggcctcgc
     3661 cccgggcgag ggcccgggcg atttcctcct ccagacccac gccgacggtc gcggcgaaaa
     3721 gcgtcaggga aagaggcggg cgttcgccct ccccgggacg aaccgacgcc aaaccggtgg
     3781 gacaggcgtc gggcgaaaac gtggcgtaga gcgccgcggt ttccagcgac gacgccacgg
     3841 aacggatctc ggtctcgacg gccttttcca gctcgggagt cgcctgggcg ttttcgctca
     3901 acgcacggag attccgcatc accgcctggg cgcggggctg gagacggaac tttttaattt
     3961 ttttgacgga aaaaaccgtc tcggccatca gtggaggggt ttgttgcgct tgcgttgcca
     4021 gaggtaaacc atcggggtgg cgatgaaaat cgtggaatag gaaccgacga tcacgccgaa
     4081 ggtcaacgcc agggagaagt cccggatcac ttcgcccccc aggaaaagca gggaaaggag
     4141 cacgatgaac accgtgaggg aggtgatgat ggtccgcgac agggtttcgt tgcaggaccg
     4201 gttgatcaaa acgtccaaag gttccttcga gtggcgaccg cgcagacgga tgttttcccg
     4261 gattcggtcg aaaataacga tcgtgtcgtt gatggaataa ccggccaggg tgagcagggc
     4321 cgccaccacg gtgatggaaa tttcccggcc ggtcagagcc atgaaaccca gggccaaaaa
     4381 gacgtcgtgc acgagggcga agacgccgga gaccccccaa atccagttgc ggaagcggaa
     4441 ggccacgtac accacgatcc cgagcatcga gaaaaacacg gccaaaatgg ccttggacac
     4501 catctgcttc cccacgaccg ggccgacgaa ctccacgcgt tccgtcgtga aggaaaaacc
     4561 ctcgaacgca cgggcaaagg cttcggtgac cttgccggcc agttcggtct tggcctcccc
     4621 ggcgcccgat ttaaatcgaa ggatgatggc gttgtgattc ggcacgctct ggagctccgc
     4681 gccggcgagc ccggccgtcg ccagggcggc ccgcacgcga tccagcgaaa tctccacgtt
     4741 ttggcccccg gcgccggcgg ccggttcgcc cgctttttga aaatacccct gaaccagggt
     4801 tccccccgtg aaatcgatgc tcatgagcga ggggccccga accaccacac cgaccaccgt
     4861 ggcggccgcc aggagtcccg aaagggcgaa aaacagtttg tggtatttga tgtaatcgaa
     4921 attgggggtt ttaaaaaatt ccattacacg ctcaatttct gcacgtcgcg gtgcattaac
     4981 cagaggtcgt aaaccgtgtg ggtgacgacg atggccgtga acatggacac cgcgagcccc
     5041 aacatcaagc tgacgccgaa ccctttgaca gggcctgtgc cgaactggaa cataaagacc
     5101 gccgcgatga tggtggtcaa attgccgtca aaaatggcgg aaaacgcctt gtcatacccc
     5161 tggtccacca ccatccgggg ggacttcccg aggcgaacct cctcccgcat gcgctccaaa
     5221 ataagcacgt tggcgtccac cgccatgccg atcgtcaaaa tgatccccgc gatgcccggc
     5281 atggtcaagg tggcgtgaag ggcggccatg gcggccaaca gcaagaccag gttcaacacc
     5341 agggccaggt tggcgagcag gcccgaccaa cggtaataaa tcaccataaa gaggaaaatc
     5401 gcccccaatc cgatggcgcc ggccagaacg cccgcgcgca ccgaatcgtc gcccagggtg
     5461 gcgcccacgg ttcgctcctc gacgatctcc aagggggcgg ggagggcgcc ggcctggagg
     5521 acggacttta agaacttggc ttcctcgggg gagaaattcc cctcgataat ggcgtgcccg
     5581 tcgggaatcc gggaccggat ggacggcgcc gactgcacca ccccgtccag gacgatggcc
     5641 aagcggcggc ccacgttggc ttcggtcaag tccgcgaagc gtttggcccc ttcgggattg
     5701 aactccaggg acacgtgggg cgaggcgccg gaaaattcgt gggcgcccat ttcgacgcgg
     5761 gcgttcgcca ggcccgctcc cgtgagagcc gcggaagcct tcacgaggta gaacgcggat
     5821 tccttgccgg ccaacaagtc ggttccggcg ggaagaagtt cggcgatttc cttgggcatt
     5881 ttgcccggcc gaagctgggc ggcagtcagg cccatctcgc gggccttgtt cagcacggcc
     5941 tcgggaacca cgtcggactc caacaggcgg aattccaaca gggcggtgcg gccgatcaga
     6001 tccttcgcct gattccggtc tttgacgccg ggcagttgaa caacgatgta cttatcgccc
     6061 tgacgaacga tcaggggctc cgaaagaccg tattggtcga tgcggttgcg gacgacttcc
     6121 atggcccggt cgatcgcttc gcgcagggtt tcgggggtgt cgtcgggcaa tttatccgtt
     6181 tgcaattcca ggagaagatg tattcccccc tgcaaatcga ggccgaggtt cagaatgcga
     6241 cccaggatcg gattccggcg atcgcgcaat tcctgctgtt tttcccggga aaggcggtaa
     6301 aagctgtagg agggaaaaag ataccaagcc gacaacagaa ccgtcgccag gatggcgagg
     6361 gacttccagt aggctttagg catgggcgcc gttggtggcg gtcggttcgg tttcctgggc
     6421 cgggtcgttg gaaacgacct gggagacgtg gctgcgggcc aggaggacct tcacgtcgtc
     6481 ggcgagtttc acttcgagaa cttttccctt gacggcctgg atggtgccgt aaaggccgcc
     6541 ctgggtcaaa acccggtcgc cgcgcttgag gttgtccagc aaagcttggt gttgtttggc
     6601 ctgtttctgt tgcggacgga tcagcagaaa ataaaagacc cccaaaatcg cgatccaagg
     6661 gaccatcatc atgagacccc cgttgggcgc gccggcggga cggccgtcgg cccacacggg
     6721 cgcggcggcg gacaaaagaa gaagcagact tgaaaatcgt ttcacaggac cccctgggag
     6781 attccggcgg gcggaccatc ggcccgggat tgacgctcca aaaacgcgtg tttaaattcg
     6841 gtgaattctc gacggacgat ggcccgccga atttgacgga ggaagtctaa cataaaggcc
     6901 aagttgtgta aagttaagag ccggagcgcc gtggtttcgc cggcccggaa taaatgggcg
     6961 aggtagcgcc gggtgtagcg tcggcacacc gggcaggcgc aggacggttc cagcggccga
     7021 ccgtcctccc gaagggccgc cacgcggacg ttgtacgacc cgtcccaagt catcacctgg
     7081 ccgtttcggg cgttgcgcgt gggccagacg caatcgaaca tgtcgatccc ccgttccacg
     7141 gcggttaaga cgtcctcggg ccgcccgacc cccattaaat agcgcggttt ggcggcggga
     7201 agctcccgca cgctcgcccc gatcatttcc cccaaaaggg cccggggctc ccccacggac
     7261 aaccccccca cggcgtagcc ggggaaatcc agggcggccg tccgccgggc gctttcggtc
     7321 cttaaggccg gaaaggtcgc cccctgcacg atggggaaaa gaagggtttt cccccgccga
     7381 tcgtccgcgg cccagcgctc ccgggcccgt ccggtccaac gaagggtgcg ctccatcatc
     7441 tcccgggcgt cggcctcggc caccgggtac ggggggcact cgtccagaca catggccacg
     7501 tccacgccca tacgaagttg ggcgtccacc acggtttccg gggtcagtcg gtgccgggcc
     7561 ccgtccacgt gggaggagaa ggacaccccc tcctcggaca cgtcccggcg gttggccagg
     7621 gaaaacacct gaaacccccc cgaatccgtg aggatggccc ccttccagga catgaacgcg
     7681 tggagcccgc cggacttttc cagaaggtcg atccccgggc gaagccacag gtggtaggtg
     7741 ttggcgagga taaaggaata gcccagggtt tcgaggtcgt cggcggagag ggttttgacc
     7801 gtggcctggg tgcccacggg catgaagatc ggcgtttcca ccgtcgcgtg ggcgagggtc
     7861 aaacgccccg cccgggcggc gccgtcgttg gtttccagct cgaaatgccc gggggcgcgt
     7921 tcggtcatgg gaccgacacg atacaaaaaa ttaacgtcgg actaggcgtt ggtcgaaacg
     7981 acgttgaggc gtttaacgtc gtcggcgagg cggtccgtgg cgtcgaacac gacgtcgccg
     8041 gccaggagga tcaggaagtt gacgaggccc ttcagatggc cgggcaggtc cgacagatcc
     8101 cactcgctgc ggttgagcaa atagaggtcc gcgggacgca attccccggg gttggacagg
     8161 ttttgcgccc gcagaagaag ggcggtgatg gatttcagcc gccccttctg ggtcggcggg
     8221 ctgacgatgg gaatgccttt caggccgccg tcgagcaacg gagccagcgc cggggttctt
     8281 cggaacaggg cgatcaaatc gacggcggag cggccttggg gcaacaccca gaccacccgg
     8341 ttgacgacgg tgttcggccg acgggcccag aattccaagg cccgccggat cgcctcgtcg
     8401 cgcccttcca atttggtttt tccgtccagg aggttggtcg tgatctcaaa cagcacgacc
     8461 cgcggggttt cccccgcccc gcgatcggtg agcgattgaa cgatgcgttc gtggaaatcc
     8521 accaatcctt cgccgcggtg gtattcgcgc atcactttca aggccttttt cggcaggttg
     8581 ggaatcaggg accacccgcc ggccagggag atcagggcgg cgacgtcgtg ggcctggcgg
     8641 gcgtcgaggc gggccccggg ccacccccga ccccgaacga tggacgccag atcccccccg
     8701 ccgcggtgaa cgcggcccag acggaaagcg atgtcgccca tggacgatcc gggggccaat
     8761 ccgaggatgg cggccaggcg ttcgcgctgg gcccgggcgg cggcccgttc gacgagggcc
     8821 cgccggaccg cctcggtccg ggaaacaacg aaacgagaat aggcgatccg gctttcggcc
     8881 ggaccaccga aaagaaggcc gttgagaaga cgctgggtgt ccgtttcggg ccggacgttg
     8941 acgcccttgg cgatcgggat ttcgaccgcc gggcggagcg cccgacgcgt cgccaaccaa
     9001 aggccggcgt tcacggccgc gtggggcaag gcgagggccg ccagggaggc ccagaaactc
     9061 aggccgccgg cgtccgccag gcccagggcc tgggcgccga gaataccgaa gaacacgccc
     9121 agggtaatgg ccattttttc ctgggagaac gccgaaagga gacggtccgc tccgcgcccc
     9181 ggggcgcccg ggacatgggc caggtgccag gccatttgat aggcggcgaa cccccagagg
     9241 ggcacccccc cggaaattcc cacggcggcg ccgaccgaaa gggacagcaa cgccggaacc
     9301 acgctttcca aggtcacggg caaaagcacc aaaccgccca aaccgccgat gcggcggtaa
     9361 atcgtctcca aaagcggcca tcggccgccg agggtttccc ggtattcccg gcgctcctcg
     9421 gccaaatggt cctcgatcaa acccagggct tcgacttcca tctccgcgtg ccgttccatc
     9481 atgtccggga aaggggattt ttcgagatcg gcccgaagtt cggcggccac cgattgaatg
     9541 gcggccaact cctggtccca gaggaccggc tcggggaaat tttcttcggt ggtgttgttg
     9601 tagcggtcca gcagggcgtg gacccggatt tgccaggttt ccagggggta atttaaaaat
     9661 tcccgggccc gggcgaggtc cgagcggacg ccgtccacca aggcccgaac agactcgacg
     9721 tccatcgggt tggggctttg ggccgcaaaa tcggccacca gcgcgtcaaa ccgcttttcc
     9781 agggcgttca aacgatcgcc gtcccggcgg aaaacaatgg tgttgatcgt gccctgatag
     9841 gggacgaggg cgttgtagac cttttggtg
//
LOCUS       DAOQLS010000006         9340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_2878_length_9340_cov_3.725902, whole genome shotgun sequence.
ACCESSION   DAOQLS010000006 DAOQLS010000000
VERSION     DAOQLS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_9340_cov_3.725902"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..5775
                     /locus_tag="PK362_00245"
     CDS             148..5775
                     /locus_tag="PK362_00245"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNC73304.1"
                     /translation="MKREMNYVVFLKPAVSLVDALYRVTSVLGSDENRANIRSMDVHM
                     DGGYRAIDLDVQIPDEAEIDRLRRELSAATLGYRLDDASQEVRERGPFGRGGASWFPG
                     LYKAYAAWLIETPILFGLLAVARDPLLAFFESLPDPGLVGLLYPALFLMGHVFNPAAF
                     REPVVYRFAVLKSILGFAFFHFEPTGLVWVAATAALVGLSAAHWKENRQNEGPVFEAA
                     RTAASVLRRRSQESGFHPVFLAWAWWRTSRRPAVPEEGETPLGADGIPSVFFEPSSAG
                     PLRESLTTGSPGGLSLGPGSIQKTAERIQKPSRRAFGLWWRRLWVIAGTVLISTLGGS
                     AKSAPVPGEAGPTVTAPGKGLPLMPWLAMVGTWGGLPRREYEALRKDLVEALTNGKWT
                     PEEVDQFGRRALDLYRDDPVQLNWLASGVRKALPKISAVGFEAEMRPLARALLERAEV
                     AYVLAVTRGIAETAIGALGDAPDAWSSIIEKLTGDLEALHRRWDNWSGPDFKTVAAVR
                     LLPRHLLQLLADGGGGRAILLARGSGVRESLESTRDHLSRAFDGLIATPLSEALPMDM
                     DHINDFALADRARKGTGGLWTVVFGLVAVGLAQYFLGTGWGDALTWANAAEGGMLSAG
                     VAGAVGALSHGGGPGWNNGGRAGWSYRRLNNGAEMWTPLGEGLSSVPEKGLIEYVGDK
                     YVVRNEIKYEKISSPVSEINDTLQINLVDAIVERARRDPARPFVVLDWGAGQGQALRE
                     IDAALKKDHPDVQNVVLVGLSNVYYSAWETLPKGVAMIYAPARNLAEVIRPESLGLVF
                     SHWSLRSIAGLRWTAYLLDLLPLLSPGGVVYSDIAPSPAALQEIGRHYFVTTNPSRPD
                     LPLLTKPLSWHAQDVGHASLVSPEHPDHNDDAVLIPERDPAAGAAPGEIPGVAVLDGY
                     GRKSSGREASSLVAETFRDWMRQLPAIPSIEQIWIDVNRTISDALGRIQKELGHLLRR
                     SRDEGAEFYPGTTQALVIPTLMPDGRHYRVIAVAVGNTHIFIFNPRDNRLYMVTDGER
                     PHSGQGKRVGESLADVDRMHRQGTPASRGARDEGILLNAIQPGHNPNKNGLAKMFVGV
                     DYHPIDDVPAGSRVLILSDGVHAALSQADLSRLLERHADKSSDQLATLLVEEAQAAVK
                     ANPKLSGDDTTAAVLDLKDPGTASMPVGPNFFSLAAMAVTGALSSLGLFYSPDASASW
                     ATLAVNAAQPLANALGPLSVATLGALPALIVAVRDRWADKSRKPAGEMIARKAVEAWG
                     LVRDGRSPALEHFPLAPDPLRQAWVGSMTAVGRLLGAAELSVSPAGALAAEARRLGRD
                     FEGMGLLELTGIRLFAFEGLVPAVLHGPGEREPFTISLLDRRSGAPIIGIPADAVAQW
                     RAEEGLPNRTRYGGNALLTTVLRESAKLAKADAEELDAVGLSREGLDSLYRRGLGPTA
                     IAELQANRYRALTSSRQGQRQERRSAANGAEAALDDLLPPTALGWTRWVTLDSVRELT
                     DRERRDRKERINGLLIGLSRQWEVQNPFASRNRTDLSALVGAATEHWSTDVFKDRLES
                     GILADLEHNDLRAMNAGLAMEILSRLRAANEGRFFVEESPGAFWTRTFGAMGLLIKLG
                     GAVYPPSADEGANDRSSLVARQDLSVRLLIEMAAESFRYDRPPTLRDLSVLDGLNLMA
                     ASYTRASRGAASLAPAADEAARALRQGRDVLVQVSRGMLEGAVWTPSEKTQMEVLREL
                     DRRLSADGEAEWKGSIVLLLDEGLRGSYVEVLRRLVENANRATGKKGDLALGLAAAGR
                     GLVLEALPGTTHLRRGDKTVIQGVEVLRLLRARNATVGTIFALRETGVEWDLKGVEDL
                     AAFVVSLAGGLVKNMTATVGEDLSKIRYLEIQA"
     gene            complement(5772..6800)
                     /locus_tag="PK362_00250"
     CDS             complement(5772..6800)
                     /locus_tag="PK362_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC73305.1"
                     /translation="MRAVWIDGHGGLDVLRVGERPRPVIGPDDVLVRVRACALNHLDI
                     WVREGLPGLKVDFPHILGSDAAGVIEEIGDNVRGWVVGQPVLAAPGLSCGTCVYCQGG
                     DDHLCDAYDILGQRSQGAYAEFVRVPAANLFPIPAPLTFEEAASLPLVFLTAWQMLVV
                     NGDLRAGQTVLVHAGGSGVGIAAIQIAKAHGARVLTTVGSIEKAERAQALGADAVVFY
                     RDTDFATGVQKETGGRGVDLVVEHIGQATFEKSLQCLTKGGRLLTCGATSGRQVQFDL
                     RQLFGRNLTVRGSRMGRRDGLGAVLEGVRKGVLKPVVDWVFPLEDAASAHRRMEERLN
                     FGKIVLSI"
     gene            7165..>9340
                     /locus_tag="PK362_00255"
     CDS             7165..>9340
                     /locus_tag="PK362_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73306.1"
                     /translation="MRPFFKKPRRSGPTVRGLAATLSVVLALEFLSAPWVEAGFWEDR
                     RRAAAPEAPLAAATLVAQLPATIDPNLPSLGAVAGHPSLPSPLTHLPSSLGRLASFEP
                     SAANAPLVVVIQDAHRVASAQLNISELLARLQAHAGELVVGVEGASGAFQLTPVRRWT
                     DAREREAVASFLVQEGVLNGAEHFGVVAGTEPFLWGVESPDEYRANVTALRESVGRRD
                     KILDRWARWKQQADAAGERMLTPAARKWNDALDQYDREGATVVDLVVALGQAAPGPMP
                     SEVAKLLGIVALEKESPRALVEEDRSRFLDRLIPTLTPAETAGLLEATAAFRLGRLSA
                     ARYYGGWRELARSHRIPWKAFTALGRYTEAVGLSETLSPGILYDQIEALRYAATAAVS
                     RTAPERSLLELNADLRMVRRLAVLELTAADWTGAKRRRADLESLELRLAALTGTWPQT
                     DARVWAEDLRPAFAFYETAEKRNETLTQNLLARAKARRADVGALVAGGFHSNGLVEAL
                     RSRGAATAVITPTIGRWNAAASAVDAFTVERTPLERLLMGQKLDLAPPSALAEQPLGE
                     SGLGALAERWALLGRGTWQLGVQNQPAGFELWAAGQPFAVRSVKFRDDHREARVALGD
                     MEADLKVVAGGTDDGEAPKELDGSAPSGRLNLQSQETVVTGEVSPVQRGRGFFLSSFR
                     WAVEKAAGWIPEALALGLSTSVGALSLAGLTWGGALALGLGGG"
BASE COUNT         1628 a         2830 c         3077 g         1805 t
ORIGIN      
        1 atcgaaacgc aagtcttcga caacgggacg tccgaagtta ccgttaaaat cgccgaaggc
       61 ccgaaccagg acccgttgct gggcctggag tttcaacggc gaatccaaaa tgttccccgg
      121 gcccagtctt accccaacga gtatttgttg aaacgcgaaa tgaattacgt cgtgttttta
      181 aagccggcgg tgtcgttggt cgacgcgttg taccgcgtca cgagcgttct gggatccgac
      241 gagaaccggg cgaatatacg atcgatggac gtccacatgg acggaggcta tcgggcgatc
      301 gatttggacg tgcaaatccc ggatgaagcg gaaatcgacc gcctgcgacg cgaactgtcc
      361 gccgccaccc tgggctatcg gttggacgac gccagtcagg aagtgcgtga acgaggcccc
      421 ttcggccgcg ggggggcttc ctggttcccg ggactttaca aagcctacgc ggcttggttg
      481 attgaaacgc cgatattgtt tggtctactg gccgttgccc gagatccgct tctcgccttc
      541 tttgaatcgc tcccggaccc ggggttggtc ggcttgcttt accccgccct cttccttatg
      601 ggccacgttt tcaaccccgc cgctttccgt gagcccgtcg tttaccgatt cgccgttttg
      661 aaatcaatcc tcggtttcgc ttttttccat tttgaaccca cggggctcgt ctgggtggcc
      721 gcgacggcgg cccttgtcgg cctctcggcg gcgcattgga aggaaaatcg tcaaaacgaa
      781 ggcccggttt ttgaggcggc ccgaacggcg gcttcggtcc ttcggcgtcg ctcccaggaa
      841 tccgggtttc accccgtttt tctggcgtgg gcatggtggc gaacctcccg acggcccgcg
      901 gtcccggagg agggagaaac cccgctcggc gcggacggga taccgtcggt cttttttgaa
      961 ccttcgtccg cgggacccct tcgcgagagt cttacaacgg gttcccccgg gggtctttcg
     1021 ttgggtcccg gttccattca aaaaaccgcg gaacgaatcc aaaagccctc tcgacgcgct
     1081 tttgggcttt ggtggcggcg tctatgggtc attgcgggaa cggttttgat ttccacgttg
     1141 ggagggtccg ccaaaagcgc tccggtcccc ggcgaggcgg ggccgacggt caccgccccg
     1201 ggcaaaggtc ttcccttgat gccctggttg gccatggtcg gaacctgggg cggtctgccg
     1261 cgccgggaat acgaagccct tcgcaaggat ctcgtggaag cgctgaccaa cgggaagtgg
     1321 acgcccgaag aggtcgatca attcggccgg cgggccttgg atctctaccg cgacgatccc
     1381 gtgcaattga attggttggc ctcgggcgtg cggaaggccc ttccgaaaat ctccgcggtc
     1441 gggtttgaag cggaaatgcg cccgctggcc cgggccttgt tggaaagggc cgaagtggcc
     1501 tacgtcctgg ccgtcacccg tggcattgcc gaaacggcca tcggggccct cggcgatgca
     1561 ccggacgctt ggtcgtcgat cattgaaaaa ctgaccggcg atttggaagc gcttcaccga
     1621 cggtgggaca attggagcgg cccggatttt aaaacggtgg ccgccgtgcg ccttttgccg
     1681 cggcatctcc tccaattgtt ggcggacggg ggcggaggtc gggcgattct cttggcgagg
     1741 ggatcggggg taagggaatc cctcgagtcc acccgggacc acctgtcccg ggcgtttgat
     1801 ggccttatcg ccacgcccct ttcggaagcc ctgccgatgg acatggatca catcaacgat
     1861 ttcgctttgg ccgatcgcgc ccgcaaaggg accggcggcc tttggaccgt cgttttcggc
     1921 ctggtcgcgg tgggtctcgc tcaatacttc ctgggaacgg gctggggcga cgccttgacc
     1981 tgggccaacg ccgccgaagg gggaatgctt tcggccgggg tcgccggagc cgtcggcgcc
     2041 ctctcccacg gcggagggcc gggttggaat aacggcggac gcgccggatg gtcctaccga
     2101 cgattgaaca acggggccga aatgtggact cccttggggg agggcctttc ctccgtccct
     2161 gaaaaaggcc tcattgaata cgtcggcgat aaatacgtcg ttcgaaacga aatcaagtat
     2221 gaaaaaatat cttcgccggt ttcggaaatc aacgacaccc ttcaaatcaa tttggtcgat
     2281 gccattgttg aacgggcccg gcgggatccc gcgcggccct tcgttgtatt ggattggggc
     2341 gccggccaag gccaggccct gcgggaaatc gacgccgcgt taaagaaaga ccatcccgac
     2401 gttcagaacg tggtattggt gggattgtcg aacgtgtatt actcggcctg ggaaacgctg
     2461 ccgaagggcg tggcgatgat ctacgccccg gcgcggaatt tggcggaggt gattcgtccg
     2521 gagagtctcg ggctcgtttt cagccattgg agcctgcgaa gcatcgcggg gcttcgttgg
     2581 acggcctatc tgttggactt gctgcctctt ttatcgccgg ggggcgtcgt gtattcggac
     2641 atcgcgccgt cccccgcggc cctgcaggaa atcggcaggc attatttcgt taccacgaac
     2701 ccttcgcgac ccgatttgcc ccttttaacg aaaccgctat cctggcacgc ccaggacgtg
     2761 gggcacgcga gcctcgtttc gcccgaacat cccgatcaca acgacgacgc tgttttgatt
     2821 ccggagcggg accccgccgc gggggccgcg ccgggggaaa ttccgggcgt ggcggtcttg
     2881 gacggctacg gacgcaagag ctcgggacgg gaggccagtt ccctggtggc cgaaaccttt
     2941 cgcgactgga tgcgtcagtt gccggcgatc ccctccatcg agcaaatttg gatcgacgtg
     3001 aaccggacca tttccgacgc cctcggccgg attcaaaagg aattggggca tttgctgcgt
     3061 cgcagtcggg acgaaggcgc ggagttttat cccggaacca cccaggccct ggtgataccc
     3121 acgttgatgc cggacgggcg gcactaccgg gtgatcgcgg tggcggtggg aaacacccac
     3181 atcttcattt tcaatccccg ggataatcgc ctctacatgg tcaccgacgg cgagcggccg
     3241 cacagcggtc aaggcaagcg agtgggagaa agcctcgcgg acgtggatcg aatgcaccgc
     3301 cagggaacgc ccgcctcccg gggcgcccgg gacgaaggga ttttattgaa cgccattcag
     3361 cccggccaca acccgaacaa gaacggactg gccaaaatgt tcgtgggggt cgattaccac
     3421 cccatcgacg acgtccccgc cggcagccgg gtgctcatcc tctcggacgg cgtgcacgcc
     3481 gccctttccc aggccgattt gtcccggttg ctggaacggc acgcggacaa atcctccgat
     3541 caactcgcca cgctgctggt ggaggaagcc caggccgcgg tcaaagccaa tccgaaattg
     3601 agcggggacg acaccaccgc ggccgtgttg gaccttaagg accccggaac ggcgtcgatg
     3661 ccggtcggcc ccaatttctt ttccctggcg gccatggccg tcaccggggc cctctcgagt
     3721 ttgggattgt tctattcgcc cgatgcctcc gcctcctggg ccaccttggc cgtgaatgcg
     3781 gctcaacccc tggccaacgc cctggggccg ctcagcgtcg ccacgttggg ggccctgccc
     3841 gccttgatcg tggcggtacg ggatcgttgg gccgacaaaa gccgcaagcc ggcgggggaa
     3901 atgatcgcgc ggaaagcggt ggaagcctgg gggttggttc gggatgggcg atcgccggct
     3961 ttggaacatt ttccgttggc gcccgatcca ttacgccagg cgtgggtggg gtccatgacc
     4021 gcggtgggac gactcctggg cgccgccgaa ttgtcggtgt cgcccgcggg cgctctggcc
     4081 gccgaggcgc ggcggttggg acgggacttc gaaggaatgg gactcctgga attgacgggg
     4141 attcgcctgt tcgcctttga aggcctggtg ccggccgtcc ttcacggtcc gggggaacgg
     4201 gagcctttca cgatctcgtt gctcgatcga cgatccggtg cgccgatcat tggaattcca
     4261 gccgacgccg tcgcccagtg gcgcgcggag gagggactgc cgaaccggac gcgctacggc
     4321 gggaacgctt tgctgacgac cgttttgcgg gagtccgcca aattggccaa agcggacgcg
     4381 gaggagctgg acgccgtggg gttgagccgg gaggggttgg attcccttta tcgacgcggc
     4441 cttggaccga cggcgatcgc cgagctgcag gccaaccgtt accgcgcttt gacgtcttcg
     4501 cgccaggggc aacggcagga aagacgttcg gcggccaacg gggccgaggc cgccttggac
     4561 gacctgttgc cgcccaccgc cctcggttgg acccgatggg tgacgctgga ttccgtacgt
     4621 gaactgaccg atcgggaaag gcgcgatcgc aaggaacgga tcaacggcct cctcatcggc
     4681 ttgagccgac aatgggaagt tcaaaacccc tttgcgtcgc ggaatcgcac cgatctgtcc
     4741 gccctggtgg gcgccgccac cgaacattgg tccaccgacg tttttaagga ccgattggaa
     4801 tccgggatcc tcgccgatct cgagcacaac gatttacgcg ccatgaacgc ggggttggca
     4861 atggagattc tttcccggtt gcgcgccgcc aacgaagggc ggtttttcgt tgaggaaagt
     4921 cccggggcct tttggacccg cacgtttggc gccatgggat tgttgatcaa actcggggga
     4981 gcggtttatc cgccgtccgc ggacgaaggg gccaatgatc ggtcgtccct ggtcgcccgc
     5041 caagatctgt ccgtccggtt attgattgaa atggccgccg aaagtttccg gtacgaccgg
     5101 ccgcccaccc tgcgggactt gtccgtcctg gacgggctga atttgatggc cgcaagttat
     5161 acgcgggcca gccggggagc ggcgtcgctc gcccccgccg ccgacgaagc ggcgcgcgcc
     5221 ttgaggcaag ggagagacgt cctggttcag gtgtcccgcg gcatgctcga aggcgcggtt
     5281 tggaccccct cggaaaagac ccagatggag gttttgaggg aattggaccg tcgattgagc
     5341 gccgacgggg aagccgagtg gaaagggtcg atcgtccttt tgttggacga gggccttcgg
     5401 ggttcttacg tcgaggtgct tcggcgtttg gtggagaacg ccaatcgggc gacgggaaag
     5461 aagggggact tggctttggg tttggccgcc gcgggccggg gtctcgtttt ggaggccctt
     5521 cccggcacaa cgcacctgcg ccggggggat aaaacggtca ttcagggagt ggaggtcctt
     5581 cgattgttac gggctcggaa cgccacggtg gggacgattt ttgcccttcg ggagacgggc
     5641 gtggaatggg atttgaaggg cgtggaagac ctcgcggcct tcgtcgtctc tctggccggc
     5701 ggtttggtga agaacatgac ggccacggtg ggggaagatt tgtcgaaaat acgctacttg
     5761 gaaattcagg cctaaatcga aagaacgatt tttccgaagt tgagccgctc ctccatccgc
     5821 cggtgggcgg aggcggcgtc ttccaaaggg aagacccaat ccaccaccgg cttcaaaacg
     5881 cccttccgga cgccctccaa aacagccccc aatccgtccc ggcgccccat gcgggacccc
     5941 cgcacggtga ggtttcgtcc aaacaattgc cttaaatcga attgcacctg tcggccgctg
     6001 gtggctccgc aggtcagcag ccgaccgcct ttggtcaggc attgaagact tttctcaaac
     6061 gtggcctgac cgatatgttc cacgaccaaa tcgacgccgc gaccgccggt ttccttttgg
     6121 accccggtcg caaagtccgt gtcccggtaa aagacaacgg cgtccgcccc gagcgcctgg
     6181 gccctttcgg ctttttcaat ggatcccacg gtcgttagga cccgtgcgcc gtgggccttg
     6241 gcgatttgaa tggccgcgat gccgaccccg ctgccgcccg cgtgcaccag aaccgtttgg
     6301 ccggccctta ggtcgccgtt gacgacgagc atttgccagg ccgtcaaaaa cacgagaggg
     6361 agggaagcgg cttcctcaaa cgttaacggc gccgggatcg gaaaaaggtt ggccgccgga
     6421 acccggacga attcggcgta cgctccttgg gatcgctggc ccagaatgtc gtaggcgtcg
     6481 caaagatggt cgtcgcctcc ttgacaataa acgcaggtgc cgcaggaaag tccgggggcc
     6541 gccagaacgg gctgtcccac aacccatccc cgcacgttgt cgccgatttc ttcgatgacg
     6601 ccggccgcgt cgcttcccag aatatgggga aaatcgactt taagtcccgg caacccttcg
     6661 cggacccaaa tgtccaaatg gttcagggca caggcgcgaa cccgaacaag cacgtcgtcc
     6721 ggtccgatca ccggccgggg gcgctcgccg acgcgaagaa cgtcgagccc gccgtgtccg
     6781 tcgatccaga cggctctcat cgaacggaaa gcctgggcat cgacggaatc gggcgatcgg
     6841 ccggccagtg agtgggcgtc atggttcctc ctcggaaggt tgaactttaa cacaagcgga
     6901 ccactttcca caacggcgtg acgccggggt tgaaggattg acttccggcc ggtccgtgct
     6961 ataatgtatt caacacaaag ttgtgttcat aaagacagcg atgtcccgcg tgacttcatt
     7021 tcgagtccgc gggattttta tttagggtcg aagggggaac ggggcttcga tccgcacgcc
     7081 gggcggctgt caccgccggc gtcagacgaa ggcgtcttgc gcgatcaaat tcgcgcggga
     7141 cgcttttttt atgggaggac ccccgtgaga ccttttttca aaaaaccccg ccgttccgga
     7201 ccgaccgtcc gcggattggc agcaactctt tccgtcgttt tggccctgga attcctttcg
     7261 gcgccctggg tggaggccgg attttgggaa gaccgtcgac gggcggccgc cccggaagcc
     7321 cccctcgccg cggcgacgtt ggtggctcaa ttgccggcga ccatcgaccc gaacctcccg
     7381 agcctggggg cggtcgcggg gcatccgtcc cttccctccc ccctgaccca tttgccttcc
     7441 tccctgggac gactcgcctc ttttgaaccg tccgcggcca acgcgccctt ggtggtcgtg
     7501 attcaagacg cccaccgagt ggcctcggcc caattaaaca tctccgaact gttggcacgc
     7561 ctgcaggccc atgcgggcga attggtggtg ggcgtcgaag gcgcctccgg cgcttttcaa
     7621 ttaacgcccg ttcgccgatg gaccgacgct cgagaaaggg aagccgtggc ctcctttctg
     7681 gtgcaggagg gggttttaaa cggggccgag catttcgggg tggtcgccgg aacggaacct
     7741 ttcctctggg gcgtcgagtc gccggacgaa taccgcgcga acgtgacggc cttgcgggag
     7801 agtgtcggtc gtcgggacaa aatcctggat cgatgggcgc ggtggaagca gcaggccgac
     7861 gccgccgggg agcgaatgct gacgccggcc gcccggaagt ggaacgacgc tttggaccaa
     7921 tacgaccgcg aaggggccac cgtggttgat ctggtcgtgg ccttgggcca ggcggccccg
     7981 ggaccgatgc catccgaagt ggcgaagctc ctgggaatcg tggctttgga aaaggaatct
     8041 ccgcgcgcgc tggttgaaga ggatcgttcg cgcttcctcg accggttgat tccaacgtta
     8101 acccccgcgg agacggccgg gctcctcgaa gcgacggcgg cttttcgtct cggacgcctt
     8161 tcggccgcgc gttactacgg ggggtggcgg gagctcgcgc ggagccatcg cattccctgg
     8221 aaagccttca cggcgctggg ccgttacacc gaagccgtcg gcttgtcgga aaccctctcc
     8281 cccgggatcc tgtacgatca aattgaggcg ttgcgctacg ccgccacggc ggccgtctcc
     8341 cgcaccgcgc cggagagaag ccttctggaa ttgaacgccg acttgagaat ggttcgacga
     8401 ttggccgtcc tggaattgac ggccgccgat tggaccgggg ccaaacgacg gcgcgccgat
     8461 ctggaatccc tggagttgcg cctcgccgcg ctcaccggca cttggccgca aaccgatgcg
     8521 cgggtctggg ccgaagacct tcgacccgcc ttcgcctttt acgaaaccgc cgaaaaacga
     8581 aatgaaacct tgacccaaaa ccttttggcg cgggccaagg cccggcgggc ggacgtgggg
     8641 gctttggtcg ccggcggatt ccattcaaac ggcttggtgg aggcccttcg gagccgcggg
     8701 gcggccacgg cggtcataac gccgaccatc ggtcgctgga acgccgccgc ttcggcggtg
     8761 gacgctttta ccgtggagcg tactccgctg gaacgccttc taatgggaca gaaactcgat
     8821 ttggcgcctc cgtcggccct cgcggagcag cctctcggtg aatcggggct gggggccttg
     8881 gccgaacggt gggcgttgtt ggggcggggc acctggcaat tgggggttca aaatcagccg
     8941 gccggttttg aactttgggc cgccggtcag ccctttgccg tgcgttccgt gaaatttcgc
     9001 gacgatcatc gggaggcccg ggtcgcgctg ggcgacatgg aagcggattt gaaagtggtc
     9061 gcgggcggga cggacgacgg cgaagcaccg aaggaacttg acgggtcggc gccgtccggt
     9121 cggctaaatc ttcaatccca ggaaaccgtc gtgaccggcg aagtgtcccc cgttcaacgc
     9181 ggccggggtt ttttcctgtc ctccttccgc tgggcggtcg aaaaggccgc gggatggatt
     9241 ccggaggcgt tggctctggg cctgtcgact tccgtcggcg ccctgtccct ggccgggctg
     9301 acctggggcg gagcgttggc cctggggctg gggggcggtt
//
LOCUS       DAOQLS010000007         8650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_3227_length_8650_cov_4.587667, whole genome shotgun sequence.
ACCESSION   DAOQLS010000007 DAOQLS010000000
VERSION     DAOQLS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3227_length_8650_cov_4.587667"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK362_00260"
     CDS             <1..365
                     /locus_tag="PK362_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC73307.1"
                     /translation="FGGGAVPCRAALAVLKELSPARLAQARKLGALFQKEISGWQKDL
                     PALREVRGAGLMIGLELDRPVADLVVACREAGLLINGTADKVLRLLPPLILTSADARR
                     GLKILRTVLAKFLSSQSH"
     gene            436..888
                     /locus_tag="PK362_00265"
     CDS             436..888
                     /locus_tag="PK362_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73308.1"
                     /translation="MGKIRPIVTGFTLIELMLVVAIIGLLSAIALPKFANLVNRSKEA
                     SIKGNLGVLRSAMSLYYADNEGWVPRVSSPGDFYVEKYIDSVPLPTLPGHRSPMANML
                     MVTNSTDCARMTFDPAYTAGSWQFCSPRILIFISCNHTDSKGIWISEW"
     gene            1035..2249
                     /locus_tag="PK362_00270"
     CDS             1035..2249
                     /locus_tag="PK362_00270"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC73309.1"
                     /translation="MKNYKKVVVAYSGGLDTSVMLKWVREHYHCDVIACCVDVGQAEN
                     YPKLKARALATGASKAYVVDARKEFVENFIWPTLKAQALYEGRYLLGTSIARPIIAEK
                     VAEIARREKADAVSHGATGKGNDQVRFELTFKAVAPELGIIAPWREWDMKGREDEIAY
                     AERHKIPVTVTKKKPYSSDANLWHISYEGGVLEDPDHPYKNDMFELTVAPEDAPNRPE
                     TVSIAFEKGIPVAVNGKRLGAVALVQTLNKIAGRNGIGRVDIVENRLVGMKSRGVYEA
                     PGATLLYFAHRELESLVLDRETAHAKELIGPRYAELVYYGQWHVPFRRALDAFVNETQ
                     KVVTGVVRLKLYKGNIVVQGRTSPHSLYWQKLASFEAEDIYNQSDAEGFINLFGLPIK
                     VAALLKRGAGRK"
     gene            2285..3622
                     /gene="argH"
                     /locus_tag="PK362_00275"
     CDS             2285..3622
                     /gene="argH"
                     /locus_tag="PK362_00275"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNC73310.1"
                     /translation="MKKAPVSPSDFVASYGFDHRLAPHDLVASMAHVEMLGRQRILSR
                     AEAAKIVAGLKKLLARVNAGGRLLKTEDVHFSIEKSLYAEIGPLAGKMHTARSRNDQT
                     VTALRMYLRERIDGLRERLRALELSFVGQAEKNIDAIMPGYTHLQPGQPISAAQHLLA
                     YAWMFRRDRERLVDLRRRDNRLPLGAAALAGTTFPIDRAWVARKLEFDGAMENSIDAV
                     SDRDFLVEFLAAGALIMTHLSRWAEELIVWSNPSFGFVTLADPFITGSSIMPQKRNPD
                     MAELLRGKTGRVVGSLNALLTLLKGTPLAYNRDLQEDKPPVFDAVDTLEACLDVSIPM
                     AQTLKLHPDRTKAACRLGFLLATDVADALARRGLPFREAHALVGRVVAECLKTNRTLE
                     DLTVEDWKIFSPLFGPWVTGVLNVEDAVAARRSLGGTAPGEIRRQIAALRKQS"
     gene            3619..4899
                     /locus_tag="PK362_00280"
     CDS             3619..4899
                     /locus_tag="PK362_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73311.1"
                     /translation="MKRLVAFFFLLGPVGAAAEESAERTFTIAQSVQVALQNNQALLS
                     AREGVRIATQRIAEARSLIWPSLAVNLNASRYRTEDHAVLAAESGSTLLPPSFNGEAD
                     NYYSGRLALRQVLYNGGRTMANTRLAQAALAQARIREEEIRRRVTVDVTGAFYGELLA
                     RKQLALSEEAQRDLQALSASLPLSDGAARAVLEGVRARLRRAQSERRRDEERASLTFL
                     STLGLELYTRVSLQGDLSAAPLRPDLAKLLARAQESRLEIRGTEYQREMDRLAVNLTE
                     AERYPVVALMAGYEVNDRAFPLDQNFWNATLNVSLPLFDGFASRARIRQTRIAANQSR
                     IERAAIEDKIAMEVRSAHADLTYWQDEAAARRDDWDRLRELYARLPAGRDPVQKGQAR
                     LELLSAAQTYWDAVHGQLIARARLENAVGLPLDP"
     gene            4889..5881
                     /locus_tag="PK362_00285"
     CDS             4889..5881
                     /locus_tag="PK362_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC73312.1"
                     /translation="MIPSFRRAATALVVALAVAAPARSSADSAPSPTDTALWDTLWSR
                     LRALPADRRPRVGLVLSGGGARGLAHIGVLKVLEREKVPVDLIVGTSVGALVGALYAS
                     GIPAGQIENLSVESGWSQLTDLSAARVVRLLVTERLLSTQKMEAYLTTHIGDKKFADL
                     KTDFACVAADLRTGEQIVLREGSVALAARASATMPGIFEPVPFRHRLLVDGGIVDNVP
                     TDVAKRLGVDLLICVYTPADFSRHTVTNVLGTLTQALYIQGQVISEERLGLADFVVRP
                     EVSEVGAMELWRSRECVEAGQRAAESAWPELRRRLVEKFFDRWLAGPTLPGASK"
     gene            5878..6843
                     /locus_tag="PK362_00290"
     CDS             5878..6843
                     /locus_tag="PK362_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC73313.1"
                     /translation="MRRFGWVLLVLTAAALRAEGLSRKERDDLAGRGAELFAASQPAA
                     ARELWEKMLAGGATGAEQRRWRPWVGRAFEAEGNFQKALTAYQEAYDRDPKNVDRLVD
                     LARVYDTVELKERALELYDRALKSDRSRTDVVLALGFLHFNAGRLAEARRYADDALRR
                     DSRDPGARRLLAQIEESQGDLEGAARRREGLLAEHPAGADAVAVGRLWARAGELELAQ
                     AAFRRAADLGVATSELLFERGAVAWRRGDVSGAEDWLGQTLAKDPGCVPAVLLLSVVD
                     WERGRGSRAQARLRGMMLAEDSPWISLRDRLITALTYGSTPGEKK"
     gene            6840..8120
                     /gene="lysA"
                     /locus_tag="PK362_00295"
     CDS             6840..8120
                     /gene="lysA"
                     /locus_tag="PK362_00295"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNC73314.1"
                     /translation="MTLNAYDTFCYRGSDLWVEGVKAADLAARLGTPLYVYSAAKIRS
                     QFERFDRAFAAFPHTICYAMKANSNLGVLSHLARLGAGADIVSGGELFRALKAGVPPA
                     RIVFSGVGKTEDEMAAALKAGILMFNIESAEEMDALERVAKRTRKAAPVSIRVNPNVV
                     VDTHHHITTGKAENKFGVSAADAEALYHKAARSPWLRVTGIQAHIGSQLLDVRPYGET
                     LDKLLALVDRLEKAGIFFHVLDIGGGLGVVYKNERSPDPADLAALLLPKLAGRNLRLF
                     FEPGRFLVAESGCLLTRVLYRKEPGHKNFVIVDAAMNDLARPALYDAHHPIWPARKSA
                     ARDYVADVVGPICESGDYLAKARHLPRPKPGDLWAVMAAGAYGFSMSSQYNTRPRAAE
                     VLVSGKKWWVVRERETLRDLIRGESMPPGLKGTR"
     gene            8117..>8650
                     /gene="dapF"
                     /locus_tag="PK362_00300"
     CDS             8117..>8650
                     /gene="dapF"
                     /locus_tag="PK362_00300"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNC73315.1"
                     /translation="MNKISFTKMHGTGNDFVLLDALRRPIRVTEALARRLCDRRFGVG
                     ADQVLILEKSKKADFAMRIFNADGSQVEMCGNGIRCLARYAHERGHTAKKEFTVDTLA
                     GVKRPRLLDGRVRVDMGEPILDAAKIPTRAAGRVLERPFAIPGTKTTLRLTAVSMGNP
                     HAVFFVPDADKIPLDTWG"
BASE COUNT         1546 a         2717 c         2813 g         1574 t
ORIGIN      
        1 ccttcggcgg cggcgcggtg ccctgccggg ccgccttggc cgttctgaaa gaattgtcgc
       61 ccgcccggtt ggcccaggcc cgaaagctcg gcgcgctctt tcaaaaggaa atttccgggt
      121 ggcaaaaaga cctgcccgcc ctacgcgagg tgcgaggggc gggcctcatg atcggcctgg
      181 agctcgatcg tcccgtggcc gatctggtcg ttgcctgccg ggaagccggc ctgttgatca
      241 acggaaccgc cgacaaagtc ctccggcttc tgccgccgtt gattttgacc tccgccgacg
      301 cccgccgggg cctcaaaatc cttcggaccg tcctggccaa attcctctcc tcccaatccc
      361 attgacccgt tcggggggac ccgaaaggct tttcaatctt cctttacggg gttatccttg
      421 gggggaggag gtcctatggg caaaatccgg ccaatcgtta cgggatttac gctcatcgaa
      481 ctcatgttgg tggtcgccat catcggcctc ctgtcggcca ttgcgttgcc caaattcgcc
      541 aatctcgtca accggtccaa agaggccagc attaaaggca atttgggggt gcttcggagc
      601 gccatgagcc tctactacgc ggacaacgaa ggatgggtcc cccgcgtttc cagccccggt
      661 gatttttacg tggagaaata tatcgatagc gtgccgttgc ccacccttcc cgggcatcga
      721 tctccgatgg cgaacatgct gatggtaacg aactccacgg actgcgcgcg aatgacgttt
      781 gaccccgcct acaccgccgg ttcctggcag ttttgcagcc cccggatttt gatattcatt
      841 tcctgcaacc acacggattc caaaggaatt tggatcagtg agtggtaatc gaaattcccc
      901 cgccgaaagg gcgcccggca tttgacgatc gggaacccaa aaaagtaaaa tcacctattg
      961 aaatcaacaa aatttggagt cgccccaccg gcggcgctgg accctttttc ccaatttaaa
     1021 ggagccgatt caacatgaag aattacaaaa aagtcgttgt ggcctattcc gggggtttgg
     1081 acacctcggt catgctgaaa tgggtgcggg agcactacca ttgcgatgtc atcgcctgct
     1141 gcgtggacgt ggggcaggcg gagaattacc ctaaactcaa agcccgggcc ctggccaccg
     1201 gcgcttccaa ggcctacgtg gtggacgcgc gcaaggaatt cgtcgagaat ttcatttggc
     1261 cgactctgaa agcccaggcc ctgtacgaag gtcgttacct gctcgggacc tccatcgccc
     1321 gccccatcat cgccgagaaa gtggcggaaa tcgcccggcg ggaaaaggcc gacgccgtga
     1381 gccacggcgc caccggcaag ggcaacgacc aagtgcggtt cgaattgacc ttcaaggccg
     1441 tcgcaccgga actgggcatc atcgccccct ggcgggaatg ggacatgaag ggccgggagg
     1501 acgaaatcgc ctacgccgag cggcacaaaa ttcccgtgac cgtcaccaaa aagaaaccct
     1561 attcctccga cgccaacctg tggcacatct cctacgaggg cggcgtgctg gaagacccgg
     1621 accaccccta caagaacgac atgtttgagt tgacggtggc ccccgaggac gcgcccaacc
     1681 gtcccgagac ggtctccatc gcctttgaaa aggggatccc ggtggccgtc aacggcaagc
     1741 gcttgggcgc cgtggcgctg gtgcaaacct tgaacaaaat cgccggacga aacggcatcg
     1801 gacgagtgga catcgtcgaa aaccgtttgg tcggcatgaa atcccggggc gtgtacgaag
     1861 cgccgggggc gacgttgctc tacttcgccc accgggaatt ggaatccctg gtcttggacc
     1921 gggaaaccgc ccacgcgaag gagttgatcg gtccccgcta cgccgaactg gtttattacg
     1981 gccagtggca cgtgcctttc cgccgggccc tggacgcctt cgtgaacgag acccagaagg
     2041 tcgtcaccgg cgtggtgcgg ctcaagcttt acaagggcaa catcgtggtg caagggcgca
     2101 cgtcgccgca ctcgttgtat tggcagaaat tggccagctt cgaggccgag gacatctaca
     2161 accaaagcga tgccgaaggg ttcatcaacc tcttcggcct ccccatcaaa gtggccgctt
     2221 tgctcaaacg gggcgcgggc cggaaataaa gccgccgccg ctggaaaatc atcgatagga
     2281 aaaaatgaaa aaagcgccgg tttccccatc ggattttgtc gcctcctacg gtttcgacca
     2341 ccggctggcg ccccacgacc tcgtggcctc catggcccac gtggaaatgc tgggacgcca
     2401 acggattctt tcccgggccg aggcggccaa aatcgtcgcg gggctcaaaa aactgctcgc
     2461 ccgggtcaac gccggcgggc ggctgctcaa gacggaagac gttcatttct ccattgagaa
     2521 aagcttgtac gcggaaatcg gccccctggc cgggaaaatg cacaccgccc gctcccggaa
     2581 cgatcaaacc gtgaccgctc tgcggatgta cctgcgggag cgcatcgacg gtttgcggga
     2641 gcgccttcgg gccctggagc tttccttcgt cggacaagcg gaaaaaaata tcgacgcgat
     2701 catgccgggc tacacccatc ttcaaccggg tcagccgatc tcggcggccc agcatttgct
     2761 ggcctacgcc tggatgttcc ggcgggaccg ggaacggctg gtcgaccttc gacggcggga
     2821 caaccgcctg cctctgggcg ccgcggcgct ggcggggaca accttcccca ttgaccgggc
     2881 ctgggtcgcg cgaaagctcg aattcgacgg tgcgatggaa aattccatcg acgccgtgag
     2941 cgaccgagac ttcctggtgg aatttttggc cgccggcgcc ttgatcatga cgcacctgtc
     3001 ccgttgggcg gaggaactca tcgtctggtc gaacccttcg tttgggtttg tcaccctggc
     3061 cgaccccttc atcaccgggt cctcgatcat gccccagaaa cgaaacccgg acatggcgga
     3121 attgcttcgg ggaaaaaccg gccgggtggt gggaagtttg aacgcccttt tgacgctcct
     3181 caaaggcacc cctctggcct acaaccggga ccttcaggaa gacaagccgc cggtttttga
     3241 cgccgtggat accctggagg cctgcctcga cgtgtcgatc cccatggccc agactctgaa
     3301 actgcatccg gaccggacca aggccgcctg ccgtttgggg tttttgctcg ccacggacgt
     3361 ggccgacgcc ctggcccggc gggggctccc cttccgggag gcccacgcct tggtcggccg
     3421 ggtcgtcgcc gaatgcctca aaacgaatcg gaccttggag gatttgacgg tagaggattg
     3481 gaaaattttt tctcccctct tcggaccctg ggtgacgggc gttctgaacg tggaggacgc
     3541 cgtggcggcc cggcgatcct tgggcggcac ggcccccggg gaaatccgtc ggcaaatcgc
     3601 cgccttaagg aaacaatcgt gaaacgcctc gttgcctttt tcttcctctt ggggccggtc
     3661 ggggcggcgg ccgaggaatc ggccgagcgg acgttcacca tcgcccaatc ggttcaagtg
     3721 gcccttcaaa acaatcaagc cctcttgtcg gcccgggaag gggtgcgcat cgccacccag
     3781 cgcatcgccg aggcccgttc cttgatttgg ccgtcgttgg cggtgaacct caacgcctcc
     3841 cgttaccgaa cggaggacca cgccgtcctg gccgccgaat cgggttcgac gcttttgccg
     3901 ccgtcgttta acggggaggc cgacaattat tattccgggc ggctcgccct tcggcaggtt
     3961 ctttacaacg gcggccggac gatggccaac acccgcttgg cccaggcggc cctggcccag
     4021 gcgcgcattc gggaggagga aatccgccgg cgggttacgg tggatgtgac cggcgccttt
     4081 tacggcgaac ttctggcccg aaaacaattg gccctgagcg aagaggccca gcgggacctt
     4141 caggcccttt cggcgtcgtt gcccctttcg gacggcgcgg cccgggcggt gctggagggc
     4201 gttcgcgcgc gcctccgccg ggcccagtcc gaacgccgcc gagacgagga gcgggcgtcc
     4261 ttgacctttt tgagcaccct gggcctagaa ctctacacac gggtgtccct gcagggagac
     4321 ctctcggccg cgccccttcg gccggacctg gcgaagctcc tggcccgggc ccaggaatcc
     4381 cgattggaaa tccggggaac cgaataccag cgggaaatgg atcggctggc ggtcaacttg
     4441 acggaagcgg agcgttaccc ggtggtggcg ctcatggccg gctacgaagt caacgaccgg
     4501 gcctttcccc tcgatcagaa tttttggaac gccaccttga acgtcagcct gccgcttttc
     4561 gacggtttcg ccagccgggc ccgcatccgg cagacgcgga tcgccgccaa ccaaagccgc
     4621 atcgagcggg cggccatcga ggacaaaatc gccatggaag tccggtccgc ccacgccgat
     4681 ttgacctact ggcaggacga ggccgccgcc cgtcgggacg actgggaccg gctgcgggaa
     4741 ctctacgccc ggcttcccgc gggccgcgac cccgtccaaa aaggccaggc gcgcctggag
     4801 cttctgtccg cggcccaaac ctactgggac gccgtccacg gccaattgat cgcccgggcc
     4861 cggctggaaa acgccgtcgg ccttcccctt gatccctagt tttcgtcgcg cggcgaccgc
     4921 gctggtcgtg gccctggcgg tggcggcgcc cgcccgatcg tcggccgatt ccgcgccgtc
     4981 ccccacggac accgccttgt gggacaccct gtggtcccgg ttgcgggcgt tgccggcgga
     5041 ccggcggccc cgggtgggat tggttttgtc gggagggggc gcccggggat tggcccacat
     5101 cggcgtgctg aaggtgctgg agcgggagaa ggttcccgtg gacttgatcg tcgggacgag
     5161 cgtgggcgcc ctggtcggcg ccctctacgc ctcgggaatt ccggccgggc aaattgaaaa
     5221 tttaagcgtg gaatcgggtt ggtcgcaatt gaccgacctg tcggccgccc gggtcgtgcg
     5281 gcttttggtg acggagcgcc ttctgtccac acagaaaatg gaggcgtacc tcaccaccca
     5341 catcggggac aagaaatttg ccgatttgaa gacggatttc gcctgcgtgg cggcggacct
     5401 tcgaaccggg gagcagatcg tccttcggga gggttccgtg gccctggccg cccgggccag
     5461 cgccaccatg ccgggcattt ttgagccggt ccccttccgc catcgcctat tggtcgacgg
     5521 gggcatcgtg gacaacgtgc ccacggacgt ggccaagcgc ctcggggtgg atctcttgat
     5581 ttgcgtttac acgccggcgg atttcagccg acacacggtc accaacgttc tgggcaccct
     5641 gacccaggcg ctttacatcc agggccaggt gatcagcgag gagcggctgg gactggcgga
     5701 cttcgtcgtc cgtcccgagg tgtcggaagt cggcgccatg gaattgtggc ggtcgcggga
     5761 atgcgtggaa gccggccagc gcgccgccga atccgcctgg cccgaattgc gccgacggtt
     5821 ggtggagaaa ttttttgatc gctggttggc gggaccgact ttgcccgggg cctcgaaatg
     5881 aggcggttcg ggtgggtgtt gttggttctc accgccgcgg cccttcgggc cgagggtttg
     5941 tcgcgcaagg aacgggacga cctcgccggc cgtggggcgg aattgttcgc ggcgtcccag
     6001 ccggcggcgg cccgggagct ttgggaaaaa atgctggccg gcggcgccac gggcgcggaa
     6061 caacgccggt ggcgcccctg ggtcggtcgc gcctttgagg ccgaggggaa tttccaaaag
     6121 gccctgaccg cctaccagga agcctacgac cgggacccga aaaacgtcga tcgattggtg
     6181 gatttggcgc gggtgtacga cacggtcgaa ttgaaggagc gcgctctgga gctgtacgat
     6241 cgcgccctca aatcggaccg ctcccggacc gacgtggttc ttgcgttggg ctttttgcac
     6301 ttcaacgccg gacgtctcgc cgaggcccgg cggtacgcgg acgacgccct tcggcgggat
     6361 tcccgggacc ccggcgcgcg gcggcttctg gcgcagatcg aagaatccca gggggatttg
     6421 gaaggcgccg cccgtcgacg ggaggggttg ttggcggaac accccgcggg ggcggacgcc
     6481 gtcgcggtcg gacgtttgtg ggcccgggcc ggcgaattgg aattggccca ggcggctttt
     6541 cggcgggccg ccgatttggg cgtggcgact tcggaattgc tttttgagcg cggcgccgtg
     6601 gcctggcggc ggggcgacgt gtcgggagcc gaggattggc tgggccagac cttggcgaaa
     6661 gacccggggt gcgtcccggc ggtattgctg ttgagcgtcg tggattggga gcgggggcgt
     6721 ggaagccggg cgcaggcgcg ccttcgggga atgatgctgg cggaagacag cccctggatt
     6781 tcattgcgcg atcgattgat caccgcgctg acttacgggt cgacgccggg agaaaagaaa
     6841 tgacgttgaa cgcttacgac acgttttgtt accggggttc ggacctctgg gtggagggcg
     6901 tcaaagccgc ggatttggcg gcgcgcctgg gcaccccgtt gtacgtttac tcggcggcca
     6961 agattcggtc ccaattcgag cgcttcgatc gggccttcgc ggcctttccc cacacgattt
     7021 gctacgccat gaaggccaac agcaatttgg gcgtcttgag ccacctggcc cggttgggcg
     7081 ccggcgcgga catcgtgtcc gggggggaac ttttccgggc actcaaagcg ggcgtgcccc
     7141 cggcccgcat cgtgttttcc ggcgtgggga aaaccgagga cgaaatggcc gccgcgctca
     7201 aggccggaat cctgatgttc aacattgaat ccgccgagga aatggacgcg ctggagcgcg
     7261 tggccaagcg aacgcggaag gcggctccgg tgtccattcg ggtcaacccc aacgtggtgg
     7321 tggacaccca tcaccacatc accaccggta aagcggaaaa caaattcggt gtgtcggcgg
     7381 cggacgccga ggccctttac cacaaggccg cccgatcgcc ctggcttcga gtgacgggga
     7441 ttcaggccca catcggctcc caattgctgg acgttcgccc gtacggcgag accctggaca
     7501 agctcctggc cctggtggac cgcctggaaa aggcgggcat ttttttccac gtgctggaca
     7561 tcggcggcgg cctgggcgtc gtctacaaaa acgagcgctc cccggacccg gcggatttgg
     7621 cggcgctcct cttgcccaaa ctcgccggtc ggaacctccg gctctttttc gaacccggcc
     7681 gctttctggt ggcggaatcg ggctgcctgc tgacccgcgt gctctaccgc aaggagccgg
     7741 ggcacaaaaa tttcgtgatt gtggacgcgg ccatgaacga cctggcccgg cccgcccttt
     7801 acgacgcgca ccaccccatt tggccggccc gcaaatccgc cgcccgggat tacgtggccg
     7861 acgtggtcgg accgatttgc gaatccgggg attatttggc caaggcccgc catttgcccc
     7921 ggccgaagcc cggggatctc tgggccgtca tggccgcggg ggcctacggg ttctccatga
     7981 gttcccagta caacacccgc ccccgggccg ccgaggtgtt ggtgtccggc aaaaaatggt
     8041 gggtggtccg ggaacgggaa acgttgaggg acttgattcg cggcgagtcc atgccgccgg
     8101 gtctcaaagg aacgcgatga ataaaatttc tttcaccaaa atgcacggca cggggaacga
     8161 cttcgttctc ctggacgccc tgcgccgccc gatccgggtg acggaggccc tggcgcgccg
     8221 tctctgcgac cgccggttcg gtgtgggagc cgaccaggtc ctgatcctgg aaaaatccaa
     8281 gaaggccgat ttcgccatgc ggattttcaa cgccgatggt tcccaggtgg agatgtgcgg
     8341 caacggcatc cgctgcctgg cgcgttatgc gcacgagcgg gggcacacgg ccaagaaaga
     8401 attcaccgtg gacaccctgg ccggcgtcaa acgaccccgc cttttggacg gccgggtccg
     8461 ggtggacatg ggcgagccga ttttggacgc ggcgaaaatc ccgacccggg cggcggggcg
     8521 ggtgttggaa cggcccttcg cgattccggg gacgaagacg acgcttcgcc tgacggcggt
     8581 gtccatgggg aacccccacg ccgttttctt cgtgccggac gcggacaaaa tccccctgga
     8641 cacctggggc
//
LOCUS       DAOQLS010000008         7925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_3683_length_7925_cov_4.550318, whole genome shotgun sequence.
ACCESSION   DAOQLS010000008 DAOQLS010000000
VERSION     DAOQLS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3683_length_7925_cov_4.550318"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PK362_00305"
     CDS             <1..884
                     /locus_tag="PK362_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNC73316.1"
                     /translation="ILMIGPPGSGKTMLARRIPGLLPPLTFEEAIETRKIHSVAGMAL
                     GALLGDRPFRSPHHTISDVALVGGGSVPRPGEVSLAHTGILFLDELPEFDRHVLEVLR
                     QPLEEGAVTVSRAAQSVTFPAGFTLVAAMNPCPCGYQGHPERECVCTPFQIRKYRTKI
                     SGPLLDRIDLHVEVPALRLAELTEEAPAAENSAAVRRRVLAARERQSARLGGAAVNAR
                     MGSKDVRTHCKLGEGSKKLLQSVVARLGLSARSFDRILKVARTIADLAGSPDLKEEHV
                     AEAVGYRALDRPVVEAV"
     gene            complement(907..1359)
                     /locus_tag="PK362_00310"
     CDS             complement(907..1359)
                     /locus_tag="PK362_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73317.1"
                     /translation="MKSGSSNSRGFTLIELMLVVAIIGLLAAIAIPKFANLVVKAKEA
                     SVKGKLGALRSALSIYYADNESYIPLGYQSFPTALTGGGKYIDSIPVIQIPTANQSHQ
                     GNDLGHQATPADVEFWAYGGAWHYSGFSGRISVNCTHTDSNGTTWSLW"
     gene            complement(1461..1970)
                     /locus_tag="PK362_00315"
     CDS             complement(1461..1970)
                     /locus_tag="PK362_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73318.1"
                     /translation="MKNRLRVLSLCLLASAALRADYTTPKPMDRLRRAFSSPIDDAAL
                     EERKKTLVEGFNRRAALIEQNFKRRQEFETQFKDAKLAFEKKQVDEQVAFIGTLKSLA
                     PNDRGALLERLNEKQGEERKAFRDQWRERLRNSQELAVKDLENFREETRKSRETDRPT
                     PQIGAPPAK"
     gene            complement(1987..2568)
                     /locus_tag="PK362_00320"
     CDS             complement(1987..2568)
                     /locus_tag="PK362_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC73319.1"
                     /translation="MALTPSTMLPLGTPAPGFQLPDVVSGKKVSLEDFAGKPALLVMF
                     LCRHCPYVKHVEKELARVARDYAPKNVAVVAISANDAAEYPDDAPAGLRAQAAEVGFS
                     FPYLYDESQSVAKAYAAACTPDFFVFNSDRRLAYRGQLDDSRPGNGRPVTGGDLRAAL
                     DDVLAGKSPRVDQKPSVGCNIKWKRTGADAAKR"
     gene            2896..3867
                     /locus_tag="PK362_00325"
     CDS             2896..3867
                     /locus_tag="PK362_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HNC73320.1"
                     /translation="MRPIQRSFLLKLWMALAVGGLGLAFSGGKSVRADDAAAPAPAVE
                     AEKPELVGASACLACHQEQGEAFKSTHHGEYLEKRKGVDFEKSCETCHGPGSLHAGAA
                     GDKANPGFATIRTFKNQRSEQVSAACFQCHKDAERSHWAGGPHARANVSCVSCHSVHN
                     AKGEHGQLVKASIKETCFQCHSDVKGQMRRSAHMPVDEDKMSCTSCHSPHGTGSPKQL
                     KAQSINQSCYQCHQDKRGPNLWEHLPVRENCLNCHNAHGSHHEKMLVAKRAFLCQRCH
                     AGTRHPSTLYDATDITNGNNRIVGQTCQDCHSNIHGSNHPSGKFLYR"
     gene            3886..6117
                     /locus_tag="PK362_00330"
     CDS             3886..6117
                     /locus_tag="PK362_00330"
                     /inference="COORDINATES: protein motif:HMM:NF023282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HNC73321.1"
                     /translation="MKSMFRSFAGLLLLAGPAALRAETKVSQEMVTGAQVVDQNNPEA
                     KFEEYREVPNGAFIDLYSMEVEGDKNDFSFTAKKVRQEDQSAAAKFNNGRLSLDVTYD
                     QIPHNWSENAKTLYTEVAPGEFRLPDGMQATFQANPGTAAWQNIMASTFAGLAHAQEL
                     ASRQDKTSVAMGYALSDKVQLGINLSMEKKNGHKLMAMPFGFSHAVEIAKPIDQTVYE
                     SNAQIGYMAGGMNLSLGYGASLYQNDINTVIWDSSRRSTDRLSSASGYSTGDQSSQGR
                     MSMQPDNQAHTFSLAGGFDLPAKSRLDADISFTQMLQNDELLPYTINTALNASTNTVG
                     IDAFDPATLPVKDANTKQTLWVQNYTLTNRSFKNVVVGFKARSEQLGNSSMEITFPGA
                     ARMDQGWSTAPISTDRFEYRKLAVGANADWDPSYRLGFTLDLTQEKATREHREYKETD
                     ERILTGKVRYRPAAWLSVRGRYVNADRKAKDLEIEDFEDASGTLIELPGLRRADIAAR
                     ERNAGDVTVQMFRGPVSVGLLGALVHDHFKPGDGDMTGGNPANLDQQYGLLSNRNARA
                     AVDLSWDATDRVGFFSFYEYEHVIGIQRSNLTNASPPVTQDAANDWSVKSTDRYDVAG
                     LGFDVDVRSDLKVTLAYDLAHSRGATDFVDLGSALATKVTPPETKTTKQDYTVKGEYQ
                     VKKDLSFELGYLFERYDVTDYATENVPVVSGSRAAQTNLLLGDSLMDYKAHVVSAKVH
                     YKW"
     gene            6263..6346
                     /locus_tag="PK362_00335"
     tRNA            6263..6346
                     /locus_tag="PK362_00335"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6297..6299,aa:Tyr,seq:gta)
     gene            complement(6641..7816)
                     /locus_tag="PK362_00340"
     CDS             complement(6641..7816)
                     /locus_tag="PK362_00340"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC73322.1"
                     /translation="MPKDFKHWLMARWLSNIAKQLLSVAVAWQVYVRTRDPLALGLVG
                     LSSAIPFIITSLWAGHVVDRSEKRGLLMAAQGALGACAAALFFSTFGRAPLLFLYGAV
                     ALKGASVSFEMISASAFAQTIVPREGFPRAAAWNLITFSTATIAGPLAGGWLLKAHSD
                     RAAYGLAMVFFLGAWTFAARLRQLPPAPGQESEAPTARVKAGLRFVKNQPLILGAMSL
                     DMVAVLFGDAVALYPIFADLLKAGPVGFGLLRASPAAGAALVSARQVARPFIHPSWRQ
                     LKRVVAAFGVFMLGFALAPHLWTAALFLFLAGIVDGVSVIIRQSLYQAHTPDSLRGRV
                     AAVSGIFISTSNEIGAFESGLSAKFLGPVGSVLFGVAMTFATVAGMGWYFRGKIREE"
BASE COUNT         1606 a         2432 c         2437 g         1450 t
ORIGIN      
        1 atatcctaat gatcggtccg ccgggatccg gcaaaaccat gctggcccgg cggattcccg
       61 gcctgttgcc gcccctgacc ttcgaggagg ccatcgaaac ccgcaagatt cattcggtgg
      121 ccggcatggc cctgggcgcc ctgctcgggg accgtccgtt tcgaagcccc caccacacca
      181 tttccgacgt ggcgcttgtg ggcgggggga gcgtgccccg gcccggggaa gtgtccctgg
      241 cccacaccgg catcctcttt ttggacgagc tccccgaatt tgatcgccac gttttggagg
      301 tcctgcgcca gcccctggag gaaggcgcgg tcacggtgtc ccgggcggcc cagtcggtga
      361 cttttccggc ggggttcacg ctggtcgccg ccatgaaccc ctgcccctgc ggctaccaag
      421 ggcacccgga acgggaatgc gtctgcacgc cctttcagat tcggaaatac cggacgaaga
      481 tttcgggacc gcttctggat cgaatcgacc tgcacgtgga agtcccggcc ctgcgcctgg
      541 cggagttgac ggaggaggcc cccgccgctg aaaattcggc cgccgttcga cgccgcgtgt
      601 tagccgcccg ggagcggcag tcggcccggt tgggcggcgc ggccgtcaac gcgcggatgg
      661 ggtccaagga cgttcggacc cattgcaagt tgggggaggg gtcgaaaaaa ttgcttcagt
      721 cggtggtggc ccgtctgggt ctctcggccc ggtccttcga tcgcattttg aaggtggccc
      781 gcaccatcgc cgacttggcc ggttcgccgg atttaaagga agagcacgtc gccgaggccg
      841 taggctatcg ggctctggac cggccggtgg tggaggccgt ttaagccgaa atcgcgcgcc
      901 gggcggtcac cagaggctcc acgtcgttcc gttggagtcc gtgtgcgtgc aattcaccga
      961 tattcgaccg ctgaacccgc tgtaatgcca ggcccccccg taagcccaaa attcaacgtc
     1021 cgccggagtc gcctggtggc cgagatcatt tccttggtgc gactgattgg ccgtcggaat
     1081 ttgaatcaca gggatcgagt caatgtactt tcctccgccg gtcagtgccg tgggaaatga
     1141 ctggtacccc aacggaatat aagattcatt gtcggcgtag tagatcgaca aagcgctccg
     1201 gagggctccc aactttcctt taacggaggc ttctttggcc ttgaccacca ggttggcaaa
     1261 tttggggatg gcgatggccg ccaggaggcc gatgatggcc accacgagca tgagctcgat
     1321 cagcgtgaat cctctactgt ttgacgagcc gcttttcata gggcccccca tttagtgtaa
     1381 aggggggaaa cgggaaattc aaggagacgg gacgggtcct cttgtcctga ccgcggaggg
     1441 aaatagcccg gaggaaaacg ctatttggcg gggggggcgc cgatttgcgg cgtggggcgg
     1501 tcggtttccc gggatttgcg ggtttcttcc cggaagtttt ccaaatcttt gacggccagc
     1561 tcctgggagt ttcgaaggcg ttccctccac tggtcccgga aggctttccg ttcctcgcct
     1621 tgtttttcgt tgaggcgctc caacagggct ccccggtcgt tgggcgccag actctttaag
     1681 gtgccgatga aggcgacctg ttcgtccact tgtttttttt caaaggccaa tttggcgtct
     1741 ttaaactggg tttcaaattc ctgccggcgc ttgaaatttt gttcgatcaa cgccgcccgc
     1801 cggttgaatc cctccacgag ggtctttttt cgctcttcca aagcggcgtc gtcgatgggg
     1861 gaggagaagg cgcggcgcag ccgatccatg ggtttgggcg tcgtgtaatc ggcccgcagg
     1921 gccgccgaag ccagcaggca cagcgataaa acgcgaaggc ggtttttcat ggattctcct
     1981 tgagagttac cttttcgcgg cgtcggcgcc ggttcgtttc catttaatgt tgcatcccac
     2041 gctgggcttt tggtcgaccc gcggggattt tccggcgagg acgtcgtcca gggcggcccg
     2101 aaggtccccg ccggtgacgg gccggccgtt gcccggtcgg ctgtcgtcca gttggccccg
     2161 gtaggcgagg cggcgatcgc tgttgaagac aaaaaaatcg ggggtacagg ccgcggcgta
     2221 cgctttggcc acggattggg attcgtcgta gaggtaaggg aacgaaaatc cgacttcggc
     2281 cgcctgggcc cgaaggccgg cgggggcgtc gtcggggtat tcggcggcgt cgttggcgct
     2341 gatggcgacg acggcgacgt ttttcggcgc gtagtcccgg gccacgcggg ccaattcctt
     2401 ttccacgtgc ttcacgtagg ggcaatggcg gcagaggaac atgaccagca gggcgggttt
     2461 ccccgcgaaa tcctccaggg agactttttt cccggaaacg acgtcgggca attgaaaccc
     2521 gggggcgggg gtgccgaggg gcagcatggt cgacggagtc aaggccattg ggcccattcc
     2581 tgaacggacg ataattccac gcaggaagta tacgtccgcc gatcggccct gtcaaagggc
     2641 cgcagcggcc tccaaaaaaa tattttcaat tatgatcgct gtcatgtacg ggattcaaca
     2701 aattgatata tacatgaggt gaatcggccg gcgaatcgcg tcgggttccc ggcttttttc
     2761 gtttcccttt tgcagtcgag cgggttcgaa ccgcttgttt ccgacgaaag tcatttcgga
     2821 ggtcccgcga aagcgggaca ggccgcaacc ccgaccccgg tcgcgcatcg accggccgga
     2881 ctcttgagga taaccatgag accaatccaa cgaagtttct tgctgaaatt gtggatggcc
     2941 ctggccgtgg gcggcctggg cctcgccttc tcggggggaa agtcggttcg agccgatgac
     3001 gcggccgctc ccgcccccgc ggtggaagcg gaaaagccgg agttggtggg ggcctcggcc
     3061 tgcctcgcct gccatcagga gcagggggag gcttttaaat ccacccacca cggcgaatat
     3121 ttggaaaaac gcaagggcgt cgattttgag aaatcctgcg aaacgtgcca cgggcccgga
     3181 tccctccacg cgggcgccgc gggcgacaag gccaatcccg gtttcgccac gattcgcacc
     3241 ttcaagaacc agcggtcgga gcaagtgtcc gccgcctgct tccagtgcca caaggacgcc
     3301 gagcggtccc actgggccgg cggcccccac gcccgggcca acgtgtcctg cgtgtcctgc
     3361 cacagcgttc acaacgccaa gggcgagcac gggcaattgg tcaaagcgtc gatcaaggaa
     3421 acctgcttcc agtgccactc cgatgtcaag ggacagatgc ggcgctccgc gcacatgccg
     3481 gtggacgaag acaaaatgag ctgcacgtcc tgccactcgc cccacggcac cggttcgccg
     3541 aaacaattga aggcccagag catcaaccaa tcctgctatc aatgccacca ggacaaacgg
     3601 gggccgaacc tctgggaaca cctcccggtg cgtgaaaact gcttgaactg ccacaacgcc
     3661 cacggatccc accacgagaa gatgctcgtg gcgaaacgcg cgttcctttg ccagcgttgc
     3721 cacgccggca cccggcaccc gagcacgctg tacgacgcca ccgacatcac caacggcaac
     3781 aaccgaatcg tggggcagac ctgccaggac tgccactcga acatccacgg gtcgaaccac
     3841 ccgtcgggca agttcttgta ccgctagagg aggagagaca tgacaatgaa atcaatgttt
     3901 cgcagcttcg ccggtctcct gctcttggcg ggacccgcgg cccttcgggc cgaaacgaag
     3961 gtgagtcagg aaatggtcac gggcgcccag gtcgtcgatc aaaacaatcc cgaggccaaa
     4021 ttcgaggaat accgggaggt tcccaacggg gcgttcatcg acctttattc gatggaggtc
     4081 gaaggcgaca agaacgactt ttccttcacg gccaagaaag tccggcagga ggaccaatcg
     4141 gcggcggcca agttcaacaa cggtcgcttg tccttggacg tgacctacga ccaaatcccg
     4201 cacaattggt cggaaaacgc gaaaaccctc tacaccgagg tcgctcccgg cgaattccgc
     4261 cttccggacg gcatgcaggc cacctttcag gccaatccgg gcacggcggc ttggcagaac
     4321 atcatggcga gcaccttcgc cggcctggcc cacgcccagg aattggccag ccgacaggac
     4381 aagacctccg tggccatggg atacgcgctg tcggacaagg tccagttggg aatcaacctg
     4441 tcgatggaaa agaagaacgg ccacaagctc atggccatgc ccttcgggtt cagccacgcc
     4501 gtggaaatcg ccaaacccat cgaccagacg gtctacgaat ccaacgccca gatcggttac
     4561 atggcggggg ggatgaacct ctccctgggt tacggggctt cgctctatca gaacgatatc
     4621 aacaccgtca tctgggacag ttcccgtcga tcgaccgacc gcctctccag cgcttcgggc
     4681 tacagcaccg gggatcaatc ctcccaagga cgcatgtcca tgcagccgga caatcaggcc
     4741 cacacgttct ccctggccgg cgggttcgac ctcccggcga aaagtcgcct ggacgcggac
     4801 atctccttca cgcaaatgtt gcagaacgac gagttgctgc cctacaccat taacacggcc
     4861 ttgaacgcct cgaccaacac cgtcgggatc gacgcctttg atcccgccac gctcccggtc
     4921 aaggacgcca acaccaagca gacgctgtgg gtgcagaact acaccttgac gaaccgctcc
     4981 ttcaagaacg tggtggtcgg tttcaaggcc cgttcggagc aactggggaa cagttccatg
     5041 gaaatcacct tccccggggc ggcccggatg gatcagggat ggtccacggc ccccatctcc
     5101 acggaccggt tcgaataccg caagttggcg gtcggcgcca acgccgactg ggatccctcc
     5161 taccggttgg gctttaccct ggacttgact caggaaaaag ccacccgcga gcaccgggag
     5221 tacaaagaaa ccgatgagcg gatcttgacg gggaaagttc gctatcgtcc ggcggcgtgg
     5281 ctttccgttc gcggccgcta cgtgaacgcc gatcgaaaag cgaaagatct cgaaatcgaa
     5341 gattttgagg acgcgtccgg caccttgatc gaattgcccg gtcttcgacg ggcggacatc
     5401 gccgcccgcg aacgcaacgc cggcgacgtg acggtccaga tgttccgagg gccggtcagc
     5461 gtcggtctcc tgggggcctt ggtccacgat cacttcaagc ccggcgacgg ggatatgacc
     5521 ggcggaaacc cggcgaacct ggaccagcaa tacggtctgt tgtcgaaccg gaacgcccgc
     5581 gcggccgtgg atctctcctg ggacgccacg gatcgggtcg gcttcttctc cttctacgaa
     5641 tacgaacacg tgatcggcat tcaacggtcg aacctgacca acgcctcgcc cccggtgacc
     5701 caggacgccg ccaacgactg gtccgtcaaa tccacggacc gctacgatgt ggccggcctc
     5761 gggtttgacg tggacgttcg atcggacttg aaagtcactc tggcctacga tttggcccat
     5821 tcccgcggcg cgacggactt tgtggatttg ggctccgccc tggccacgaa ggtgactccg
     5881 cccgaaacga aaacgaccaa acaggactac accgtcaagg gggaatatca ggtcaagaaa
     5941 gacctttcct tcgaactggg ttacttgttc gaacgttacg acgtgacgga ctacgccacc
     6001 gaaaacgtcc cggtggtgtc cggcagtcgg gccgcccaga cgaacttgct tttgggcgac
     6061 tcgttgatgg attacaaggc tcatgtggtg agcgccaaag tccattacaa gtggtaagcc
     6121 aaaccatctt ctgacaccac gatcacaacg gcgcgccctc tccccgacga cgggggggag
     6181 ggcgcgcctc ttttctcgcg cttgacgggg gaatcgacac cgtgatataa cagggattcc
     6241 ccgccgcgta ttttcccttt ccgcacaggt ggcagagcgg tcaaatgcac cagactgtaa
     6301 atctggcggg cctcgcccta cgaaggttcg aatccttccc tgtgcaaatt taaaaggcgc
     6361 ccctcgtggg cgccttttga atttgcggag atctcaggat ttgaacttgg ttcgttgccg
     6421 ctaggcaacg tcccggttgg taggacgggg cgcggtcaag gcgaaagacg aagtctttcg
     6481 cttaatggag tggcgggctt ccgctcgacg taggcctggg gccgttatcc ttccctgtgc
     6541 aaatttccat gccgctaggc atggcccctg ttcgtggaaa ggggccggag ccccagcggc
     6601 gccttttgaa tttgcgggca tcccaggatt tgaacgtcgt ttattcctcc cgaattttcc
     6661 ctcggaaata ccaccccatc ccggccaccg tggcgaaggt catggccacg ccgaagagca
     6721 ccgagcccac gggccccagg aatttggccg acaggcccga ctcaaaggcc ccaatctcgt
     6781 tggaggtgct gataaagatg ccgctcacgg ccgccacccg cccccgaagg gagtccgggg
     6841 tgtgggcttg gtaaaggctt tgtcgaataa tcacgctgac gccgtccacg attccggcca
     6901 ggaatagaaa aagtgccgcc gtccaaagat ggggagccag ggcgaaaccc agcataaaca
     6961 cgccgaaggc cgccaccacg cgttttaatt ggcgccagga ggggtggatg aacggccggg
     7021 ccacctgacg ggcggaaacc agggcggccc cggcggcggg ggaggcgcga agaaggccga
     7081 atcccaccgg gccggccttc agaaggtcgg caaaaatggg ataaagagcc acggcgtcgc
     7141 cgaaaagcac ggcgaccatg tccaacgaca tggcccccag gatcaacggt tgatttttga
     7201 cgaaccgaag ccccgccttg acgcgggccg tcggcgcttc gctttcctgc cccggcgcgg
     7261 gcggaagttg tcggaggcga gccgcgaagg tccaggcccc taaaaagaaa accatggcca
     7321 gaccgtaggc cgcgcggtcc gaatgggcct tcaacagcca tccccccgcc agggggcccg
     7381 cgatggtggc cgtggagaag gtaataaggt tccaggccgc ggcccgggga aatccttccc
     7441 gggggacgat ggtctgggcg aaggcgctgg ccgagatcat ttcaaagctg accgaagccc
     7501 ctttcagcgc cacggcgccg taaagaaaca acaaaggggc ccgtccgaaa gtggagaaaa
     7561 agagggcggc ggcgcaagcg cccagggccc cctgggcggc catcaacaac cccctctttt
     7621 cgcttcggtc caccacgtgg cccgcccaaa gggaggttat tatgaaagga atcgcgctgg
     7681 aaaggcccac cagacccagg gccagcgggt ccctggttcg cacatagacc tgccaggcca
     7741 cggcgacgga aagcagttgc ttggcgatgt tggaaagcca ccgggccatc agccagtgtt
     7801 tgaaatcctt cggcatagag cgtcaggata gtagatggtt cccgagcgcg caacttgtca
     7861 cccccattcc ataatggtat ccttacccct tcggaaacga aaatttggga aaaagagccc
     7921 cccgg
//
LOCUS       DAOQLS010000009         7242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_4244_length_7242_cov_4.389871, whole genome shotgun sequence.
ACCESSION   DAOQLS010000009 DAOQLS010000000
VERSION     DAOQLS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4244_length_7242_cov_4.389871"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PK362_00345"
     CDS             complement(<1..512)
                     /locus_tag="PK362_00345"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNC73323.1"
                     /translation="MWIVAVTAMVLGLSAFGFFDPFENMSMDFRFARRGPRPADSRVV
                     LIDIDEQSIKALGLFPWPRETHARLIERLVQAGAAAIAFDVLFVDEDTQNAPGDAALG
                     RAAEESGRTVFGEVFSDFRRDLPTRLETPIEAVRGPSVQYGFVNAFPEEDGVLRRSAL
                     WLEFEGKKIPS"
     gene            complement(533..1510)
                     /locus_tag="PK362_00350"
     CDS             complement(533..1510)
                     /locus_tag="PK362_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC73324.1"
                     /translation="MKPGVYVVTGGAGFLGSHLSDHLLRRGHRVVGVDNFLTGASDNI
                     AHLAGHPRYRFIKHDVTQDLYLHGPVDGILHFASPASPRDYLEFPIQTLKVGALGTHK
                     MLGLAKAKNARFLLASTSEVYGDPQVHPQREDYWGHVNPVGPRGVYDEAKRYAEAMAM
                     AYHRAHGVKVRIARIFNTYGPRMRKNDGRAVPEFIDAALNGKPIPVFDRGQQTRSLCY
                     VEDEVEGLLRLLFSAHVGPMNIGNPREITILEIARLVKKLTGSRSPIVHKPLPVDDPK
                     VRQPNIAFARRALRWEPTVPLEEGLRRTVEWFQTAADAVPAPKRSRKRP"
     gene            complement(1507..2430)
                     /locus_tag="PK362_00355"
     CDS             complement(1507..2430)
                     /locus_tag="PK362_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73325.1"
                     /translation="MTIPKTPQRNIPLPHAERARSLMLLGFGGLLISAFLARGGREPF
                     ASGFVALAAWSYLLLVDGWVYRHRGESLIQSHPRRFLFLALWSLVFKLFNEMANLRLG
                     YWAHGVTPASRGAAWILGAALLAAAVPVLLETADLIESAGVFKSVTVPPLHRAPSVFV
                     VTGGLIFAVLALGWPRLFSPLWWAVPLFLCDPLNERLAEPSLLSDARVGQVRKIALVG
                     LAGLLTGVLWEVTNVVAGPHWIYPTLDNPVRFLELPVAGYLLYPFMALSAYASSVTAV
                     ALWDRSRPWVRILLAFAAAVGVVGALARVWP"
     gene            complement(2578..3189)
                     /locus_tag="PK362_00360"
     CDS             complement(2578..3189)
                     /locus_tag="PK362_00360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC73326.1"
                     /translation="MKSLPDFREPKTAAVPDPSAEWGEEHFENYGGPGRSYRRTWKQY
                     NSVTDCVRIFSQIEGLRIQSVIVLGAAMGDILKFFESSWGTRPHGCELSRWAYRQIPH
                     PYRRRIKNQDMKHYVAECQKKVDVVYANSFMYLNEEDVARVLKECARIGRFLFVDLSY
                     SESYAENDPFRRVLKPHRWWRNQFREAGFLPFKRHRRLWQSAR"
     gene            complement(3186..4193)
                     /locus_tag="PK362_00365"
     CDS             complement(3186..4193)
                     /locus_tag="PK362_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNC73327.1"
                     /translation="MASVPCVPAEAGRSTDSTAVRPAGAPKPPKRFKRVYIEISNVCN
                     LHCTFCPDVSRPKSFMDPVLFNEVAKAVAPLTEEVAFHLLGEPLLHPALESLVDICRA
                     LDLPVHLTTNGLRLTPARAKTLLNPIFRQINFSLQSFDDNFPGHDNTDYLRAIFEFTA
                     TALRRRPDLYLNYRLWNDGAPGFGAANERWIAAIKKNLGVDVVPARDLRWRKSVPLKS
                     RVSLHFDSRFEWPRPDRPARSDRGFCHGLSTHFGVLTDGTVVPCCLDKDGVIDLGNLR
                     TQNLHEVLQSARAEALRDGFQKAVLVEDLCRKCPFIARFDAKARRIIAGQRSTPARPR
                     P"
     gene            complement(4210..4977)
                     /locus_tag="PK362_00370"
     CDS             complement(4210..4977)
                     /locus_tag="PK362_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNC73328.1"
                     /translation="MSDRRSPPRAPVLVFRHVPHEGLGTLAPALRAAGYPLRVIDIAR
                     GRPALPSPRELAALIVMGGPMGVYEKTKHPFLRREITYIRRALRAKTPVLGVCLGAQL
                     IAHALGQRVYPNRQKEIGWYPLHRTAAARRDALFAHFPKTATVFQWHGDTFDLPVGAT
                     LLATSPRCRHQAFRWGNRVYGLQFHVEVDPAMVREWLSQPGVDRELRSVDGRARREIR
                     RHRPARWRGLQRLAGPLFQGWAGVLNGTPPPRASRPR"
     gene            complement(4974..5912)
                     /gene="ispH"
                     /locus_tag="PK362_00375"
                     /gene_synonym="lytB"
     CDS             complement(4974..5912)
                     /gene="ispH"
                     /locus_tag="PK362_00375"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNC73329.1"
                     /translation="MKILLARPRGFCAGVVRAVDIVDIALQKFPAPIYVRKEIVHNRA
                     VVDDFKSRGVVFIDTLDEAPPQSLVVFSAHGVSPEVRRRAEERGLRVIDATCPLVTKV
                     HLEVHRFRREGYRLLLVGHREHDEVEGTLGEAPGEITLVENAEDVARLPFPPDEKIMI
                     LTQTTLSLDETRGVILAIKNRFSNAHTPPKDDICYATQNRQDAVKDLLRRGIDLLLVV
                     GSKNSSNSQRLVDVARQSGCAAHLIDGAGEIDPAWLAGVKTVGLTAGASAPEHLVQGV
                     VALLTGRGGRVEEVTVREEDVHFSLPKELAGLTPTR"
     gene            complement(5909..6487)
                     /locus_tag="PK362_00380"
     CDS             complement(5909..6487)
                     /locus_tag="PK362_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73330.1"
                     /translation="MTSVSTQPARSTLRTGKVIAERPQILTPDAFVQRFKDFGEDSSA
                     YERFLKDHFSDSLRGLEYNFRNILETTEPHHTDARELARNIRKDLDDRNAARAAVILG
                     PESGWGFALMKFILEETSQSFAVNVRELDERGLFNPPDAEERRRREIEILLRRAESDP
                     SLVKPLADLLNRHGLFNDYQDAFFRLVKRGAS"
     gene            6564..6761
                     /locus_tag="PK362_00385"
     CDS             6564..6761
                     /locus_tag="PK362_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73331.1"
                     /translation="MASETRTRAGAAADEVRGEVDAAPLVPTADVIRAVDAFIKGGLK
                     DVTFVGTPPPDSTLDKILSGK"
BASE COUNT         1338 a         2158 c         2525 g         1221 t
ORIGIN      
        1 gaggggattt ttttcccttc gaattccaac cacagggcgc tccgtcgaag gacgccgtcc
       61 tcctcgggaa aggcgttgac gaaaccgtat tggacggagg ggccccgaac ggcctcgatg
      121 ggggtctcca ggcgcgtcgg gaggtctcgg cgaaaatccg agaaaacttc cccgaagacg
      181 gttcgcccgg attcctcggc ggcgcgtccc agggccgcgt ccccgggggc gttttgagtg
      241 tcttcgtcga cgaaaagaac gtcgaaggcg atggccgcgg ccccggcctg gacgagtcgt
      301 tcgatcaagc gggcgtgggt ttcccgcggc cacgggaaaa gccccaacgc cttaatggat
      361 tgttcatcga tatcaatgag gacgacgcgg gaatcggcgg gtcggggacc ccgtcgggcg
      421 aaacggaaat ccatggacat gttttcgaag gggtcgaaaa aaccgaatgc ggacaacccg
      481 aggaccatcg cggtgacggc gacaatccac accccgtggg ccgggggacg ttttatgggc
      541 gtttcctcga tcgtttcggg gcgggaacgg cgtcggccgc ggtttgaaac cattccaccg
      601 tccgccgaag tccttcttcc agggggacgg tcggctccca tcgaagcgcg cggcgggcga
      661 aggcaatgtt gggctgacgg actttggggt cgtccacggg caggggcttg tggacgatgg
      721 ggcttcggct gccggtcaac ttcttgacca ggcgggcgat ttccagaatg gtgatttccc
      781 gggggttgcc gatgttcatg ggcccgacgt gggcggagaa cagcaggcgc agcagtcctt
      841 cgacctcgtc ctccacgtag cacaggcttc gcgtttgttg gccccggtcg aacacgggga
      901 tcggctttcc gttcagcgcg gcgtcgatga actcgggaac ggcccggccg tcgttcttgc
      961 gcatgcgggg accgtaggtg ttgaagatcc gggcgatgcg caccttgacc ccgtgggccc
     1021 ggtgataggc catggccatc gcttcggcgt accgctttgc ttcgtcgtac accccccggg
     1081 gacccacggg attgacgtgg ccccaatagt cctcccgttg ggggtggacc tggggatcgc
     1141 cgtacacctc ggaggtcgag gccaggagga aacgggcgtt tttggccttg gccagaccca
     1201 gcattttgtg ggtgcccagg gcgccgactt taagggtttg aatgggaaat tccaaataat
     1261 cccggggcga agccgggctc gcgaaatgga gaattccgtc cacgggaccg tgcaggtaca
     1321 ggtcctgggt gacgtcgtgc ttgatgaatc ggtagcgggg gtggccggcc aggtgggcga
     1381 tgttgtcgga cgcgcccgtc aaaaaattat cgacgccgac aacgcgatgc ccccggcgaa
     1441 ggagatggtc gcttaagtgg gaccccagga accccgcgcc cccggtgacg acgtaaacgc
     1501 cgggtttcac ggccacaccc gggccagcgc gcccacgacg cccaccgccg cggcgaaggc
     1561 caggagaatc cggacccacg gtcgggagcg gtcccaaagg gccacggccg ttaccgagga
     1621 ggcataggcg ctcagcgcca tgaacgggta gagcagataa ccggccacgg gcagctcaag
     1681 aaaccgaacg gggttgtcca aggtcggata aatccaatga ggtccggcca cgacgttggt
     1741 gacttcccag aggacgccgg tgaggagccc ggccaaaccc accaaggcga ttttgcgaac
     1801 ttgacccacc cgggcatcgg acagcaggga aggttcggcc aaacgctcgt tcagcgggtc
     1861 gcaaagaaac agcggaacgg cccaccacag cggcgaaaac agacggggcc accccaaggc
     1921 gagaacggcg aaaatcaggc cgccggtcac gacgaaaaca ctgggggcgc ggtggagggg
     1981 cggcacggtg acggatttaa aaacgcccgc ggattcaatc aaatcggcgg tctccaggag
     2041 gacgggcacc gccgccgcca agagcgcggc gccgaggatc cacgccgccc cccgggaggc
     2101 cggcgttacc ccatgggccc aatagcccag gcgaaggttg gccatttcgt tgaacagctt
     2161 gaacaccagc gaccacaagg cgagaaaaag gaaacggcgc ggatgggatt gaatcaggga
     2221 ttcgccccga tgacggtaaa cccacccatc caccaggagg aggtaactcc aagcggccaa
     2281 ggccacgaac ccggaggcga agggttcccg gccgccgcgg gccagaaaag ccgagatcaa
     2341 caacccaccg aatcccagaa gcatgaggct tcgagcgcgt tcggcgtggg gcaacgggat
     2401 gtttcgttgt ggggtctttg gaatggtcaa ggttctctcg gcgtctcacg cctcgaagtc
     2461 gggggacagt ttacctcatc ggggagccgg ccgaaaggcc ccggcctcaa atcccggaaa
     2521 cgccgtgaag gggcggcgaa tccggcggtc cggccatcgg caccggcgga ccgtcggtca
     2581 ccgagccgat tgccacaacc gccggtgtct tttgaacggc aaaaagccgg cttcgcggaa
     2641 ttggttccgc caccagcggt ggggtttgag gacccgtcgg aaggggtcgt tctcggcgta
     2701 gctttccgaa tagctcaaat ccacgaacag gaagcgacca atgcgggcgc attccttcag
     2761 tacccgggcg acgtcctcct cgtttaagta catgaacgaa tttgcgtaaa cgacgtccac
     2821 ttttttttga cattcggcca cgtaatgctt catgtcctga ttcttgatgc gccggcggta
     2881 ggggtgggga atttggcggt aggcccagcg gctcagctcg cagccgtggg ggcgggtgcc
     2941 ccaggaggat tcgaagaatt tcaaaatgtc gcccatggcg gccccgagca cgatgaccga
     3001 ttggattcga agcccttcga tttgggaaaa gatccgtacg caatcggtca cgctgttgta
     3061 ttgcttccaa gttcgacggt aactccgtcc cggaccgccg tagttttcga aatgttcctc
     3121 cccccattcg gcggaggggt cggggaccgc cgcggttttt ggttctcgaa agtcggggag
     3181 ggatttcacg ggcgggggcg ggcgggcgtt gaacgttgcc cggcgatgat ccgccgggct
     3241 ttggcgtcga accgggcaat gaaggggcat ttccggcaaa gatcttcgac aagaacggcc
     3301 ttctgaaacc cgtcgcgaag ggcctcggcc cgcgcgcttt gaagaacctc gtggaggttt
     3361 tgggttcgga ggttccccaa gtcgatgacg ccgtctttgt ccaggcagca gggaacgacg
     3421 gttccgtcgg tcaaaacgcc gaagtgggtg gagagtccgt gacaaaaacc gcggtcggag
     3481 cgggcgggcc ggtcggggcg gggccattca aaccggctgt cgaaatgaag cgagacccgg
     3541 ctcttgagcg ggacgctttt cctccatcga aggtcccgcg cgggcacgac gtcgacgccc
     3601 aggttttttt tgattgcggc gatccatcgc tcgttggcgg cgccgaagcc cggcgcgccg
     3661 tcgttccaca ggcggtaatt gaggtaaagg tcgggccggc gccgaagggc cgtcgcggtg
     3721 aattcaaaaa tggcccggag atagtcggtg ttgtcgtggc ccgggaaatt gtcgtcgaag
     3781 ctttgaaggg aaaaattgat ttgccggaag atcggattca gaagcgtctt ggcccgggcc
     3841 ggggtcagac gaaggccgtt ggtcgtgagg tggacgggaa ggtcgagagc ccggcagata
     3901 tccaccagcg attccaacgc cgggtggaga agtggttcgc ccaggagatg gaaggcgact
     3961 tcctccgtca gcggggccac ggccttcgcc acctcgttga ataagaccgg gtccatgaag
     4021 cttttggggc gggagacgtc gggacaaaag gtgcagtgca aattgcacac gttgctgatt
     4081 tcgatgtaaa cccgtttgaa tcgcttgggg ggtttgggcg cgcccgcggg gcggaccgcc
     4141 gtcgagtccg tcgatcgtcc ggcctccgcg gggacgcaag ggacggaggc catcgcggcg
     4201 ggggcggaat cagcgggggc gcgacgcacg ggggggagga gttccgttca ggacccccgc
     4261 ccatccttga aaaagcggtc cggccaatcg ttgaaggccc cgccagcggg cggggcgatg
     4321 acggcggatt tcccgccggg cgcgtccgtc gacgctccgg agttcccggt ccacgccggg
     4381 ttgggaaagc cattcgcgga ccatggccgg gtcgacctcc acgtgaaact ggaggccgta
     4441 gacccggttc ccccaacgaa aggcttggtg acggcaacgg ggagaggtgg ccaaaagagt
     4501 cgcgccgacg gggagatcga aagtgtcccc gtgccactga aagacggtgg cggtttttgg
     4561 aaagtgggcg aacagcgcgt cgcggcgggc cgccgcggtc cgatgcaggg gataccagcc
     4621 gatttccttt tggcggttgg ggtagacccg ttgcccgagg gcgtgggcga tcagttgggc
     4681 gcccaaacac acgcccagga ccggtgtctt cgcccgaagg gcccggcgga tgtaggtaat
     4741 ttcccggcgg agaaaggggt gttttgtttt ctcgtagacc cccatgggcc ctcccatgac
     4801 gatcagagcg gcgagttcgc ggggagaggg cagggccggg cgcccccggg cgatgtcaat
     4861 gactcgtaag gggtatccgg ccgcgcgaag ggcgggggcg agggtcccca ggccttcgtg
     4921 ggggacgtgg cgaaaaacga ggacgggagc gcgcggcggt gagcgccggt cgctcatcgg
     4981 gtgggcgtga gcccggccaa ttccttgggc agggaaaaat ggacgtcctc ttcccggacg
     5041 gtgacttcct ccacccggcc gccgcggccg gtcagcaggg cgaccacgcc ttgcaccaga
     5101 tgctccgggg cggaggcgcc ggcggtgagc ccgacggttt tgacgcccgc gagccaggcg
     5161 ggatcgattt cgcccgcgcc gtcgatcaaa tgggcggcgc aaccggactg gcgggccacg
     5221 tccaccaggc gttggctgtt ggagctgttt ttggagccga cgaccaggag caaatcgatg
     5281 ccccgtcgaa gcaaatcttt gaccgcgtcc tgacggttct gggtggcgta gcagatgtcg
     5341 tctttgggcg gggtgtgcgc gttggagaag cgatttttga tggcgaggat gacgccccgg
     5401 gtttcgtcca ggctcaaggt ggtttgggtc aggatcatga ttttttcgtc cggggggaag
     5461 ggcaggcggg cgacgtcctc ggcgttttcc accagggtga tttcgcccgg ggcttcgccg
     5521 agggtgcctt ccacctcgtc gtgctcccgg tgccccacca gcaagaggcg gtatccttcc
     5581 cggcggaacc ggtggacctc caaatggact ttggtcacca gcgggcaagt ggcgtcgatc
     5641 acccgaagcc cccgttcctc ggcccgacgc cgaacctccg gggatacccc gtgggccgaa
     5701 aagaccacga ggctttgggg cggggcctcg tccagggtgt cgatgaaaac cacgccccgg
     5761 gatttaaaat cgtcgacgac ggcccggttg tgaacgattt ccttgcgaac gtatatcggg
     5821 gccggaaatt tctgaagggc gatgtcgacg atgtcgaccg cgcgcaccac gcccgcgcag
     5881 aacccccggg ggcgggccag gaggatcttc acgacgcccc gcgtttgacg agtcggaaaa
     5941 aggcgtcttg gtagtcgttg aaaaggccgt gccggttcaa gagatcggcg aggggtttga
     6001 ccagcgacgg gtccgactcg gcccggcgca gaaggatttc gatttcccgc cgtcggcgct
     6061 cctcggcgtc cgggggattg aacaggcccc gttcgtccag ctcgcggacg ttgacggcga
     6121 agctctggga ggtttcttcc aggatgaatt tcatgagcgc gaagccccag cccgattcgg
     6181 ggccgagaat gacggcggcg cgggcggcgt tgcggtcgtc caaatccttg cggatgttgc
     6241 gggccagctc ccgggcgtcc gtgtgatggg gttcggtggt ttcgaggatg ttgcggaagt
     6301 tgtattccag gccccgcagg ctgtcggaga aatgatcttt caggaaccgt tcgtaggccg
     6361 aagagtcctc cccgaaatcc ttgaaccgtt gaacgaaagc gtcgggggta aggatttgag
     6421 ggcgttccgc gatgactttc ccggtccgca gggtggaccg ggccggttgg gtggatacgg
     6481 acgtcaccag ctgataggaa atctcacttt ggccgaaggt ggccagccga tgtcggggaa
     6541 accggagaac ccggatctcc cgcatggcct ccgagacgcg cacccgagcg ggcgccgcgg
     6601 ccgacgaggt caggggcgag gtggatgccg cgccgctcgt cccgacggcg gacgtgatcc
     6661 gcgccgtcga tgcgttcatc aagggcggtc tcaaggacgt gacgttcgtc ggcaccccgc
     6721 cgccggacag cacgctcgac aagatcctga gcgggaagta gtccccacct gcgaaacccg
     6781 ccctccccgg ctcctccggg gagggcaccc gcaagggatc gtccgcgatc tccgggcgat
     6841 ccggggaccg gctccggccg gcgcaccccg gctccgttcg gacctcgcag atccgaactc
     6901 cgacgcactc cacgagcggc ccgtccacac ggcgggccgc tcgttccgtc tccgcgccgc
     6961 gctcccgcgg cgccgcagtc cgcacgcgac atcgagagcc cgtcccgtcc ccgagccgac
     7021 gacggaagga gatccccatg agggaccgag ctctcgagac ccgaccgcgc tcccccgccg
     7081 gcgccacggc gcgccgcgcc tcgctccgcc gaggtgacct gtgagcgcgc gcaacgagcc
     7141 gacccggctc aacatgacgc cgatgatcga cgtcgtgttc cagctcatcg tgttcttcct
     7201 cgtgtcgatg aagttcaaga cgctcgacat gcggatcgac ac
//
LOCUS       DAOQLS010000010         7242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_4245_length_7242_cov_3.997635, whole genome shotgun sequence.
ACCESSION   DAOQLS010000010 DAOQLS010000000
VERSION     DAOQLS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4245_length_7242_cov_3.997635"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..7204)
                     /locus_tag="PK362_00390"
     CDS             complement(<1..7204)
                     /locus_tag="PK362_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC73332.1"
                     /translation="MLSVGTSSFWATPLQSLRELAGASAAVLGPLVGGFTAGLLGHSG
                     VNLARRREGRAFLTGNDSKGRGFFGNLVSLATDLMGLKPTNTIVEVGPAGKPITLADT
                     NHITGSREPTIGYKINYDPIYDRDLLERSKAIQTWALNQENYGLIRGFSGEIDGPRSG
                     QHSGRTWWAAVPLAAKDKIVEALKAGNLADPDVNRYLSHNVRRYLASPKGQIWVTTLH
                     DLVSQNPLESVEQDSLGDLVAHFTETNMPLNGVHAFSLEWNPGFELDFQRNIHPETGY
                     DEVFPKDFDFFQWRLTRHISEALHRDLPGDLQKTAGEHTVAVFLNEDKTAVERVVVGS
                     TQFGWRFSPETNAMVLPGDESHPDGRASVEEVQHRMIQLNVRTDETGRWQTERVRGFF
                     NDLQLANHLLNRSGRVTNEGPATEESGPLVVSTVQPLPLMVVHQMGLPVGEFVPEKFV
                     AELMNDRELGRIFLMGTETDEGHRLYQHTAMVLRQFERYFAASPRFQAALTKAGVDLG
                     FFRLFMALHDIGKPEARAGRVGENEFDHTSRLIRRYFERHGLSSKQLDLALSLAGGDP
                     IGQYLRANPPTSTEFLSARDPLFIALAQGTEPSPLAYKAAAEISANARRLELDVETYW
                     ELLTAYYQSDAGAYTTDAYYAENGRLNYGPTSLNALFDFTRTDGSFISPPWQLKMEPL
                     QRLVLGVTPPTTTPPAALPEITGPTLLTPESGPHRLPQFPSRLLLANVHPVSLRIDHG
                     NLVATGPDGSTAVLQPGQTLSIGRSPDENSVGLVIKAQDVKNVSRRHATVQWDGQTAL
                     VADLSPNGTYVKPGDPLGDEFDAKLRAALQSEIPAVMDGQTVKVNLSVYTNGEAARVF
                     REDEAIPARDLQGFEPHEITVFARSADGHTVDATIGRVRDGLGSKIQTLQSLAVGQVR
                     PLDFSAEFGTVGAAFSRVTEDVATALAALAKPRGRADIENIVYSLGANAETLLTNQTT
                     RRPIPYNLEYFSALINAWRNVQETVLLGGQSKFPKLSMSVPQKNLPFPIVAKDTHLVV
                     ESVANGKITLLLPSGERLTGSTIEFGRGNLGLGAVSGISAKHFTLSLVGSDVVILDND
                     STNGTLVPSSTVAPRGPPAATISGLVPLIGRLYGLNPANPVDLNRIVNEKAPLVETVV
                     TGAFYYAAVGLLSALGSAVGLHIDHAFFAATGLFALVNLAFGFSHRSVYRFKPPVPGV
                     NRLGRWEAEPAPASRRERWGLALRGAAIHAPLLLAFMDPLGGLFLAPIAALTSVGLHA
                     VHNRLAAERGWSALTAGGVDQKTTPPTADLVYMRANTDRRRVDLAMDGLGYTTDLHYL
                     LAFHQGTIPEGSQPNPEETESLEREFAVAQALTQLLGYDSLAELLVANGEKKIDIRPE
                     PVLRYPTSRELILDILTRQGVPGPARREVTSRVDAESARGVLDRLADFLGYEAVKTQT
                     KDGEKVTPARQGLIGDLPLNLAFHRPRTDPLKVSDQRDFLPPFLKRYRLPAVAEFTLP
                     RLGEVRKPGGKLQPFENQFTLGPRGVEKRERTDSLLTAPLLGAAWVGQGNLAVKVWLS
                     GGVLHAQRFKHTDNQLTALDAQPIVVARALFEKGIDPTESLGLGSELFFTEKGVGLTS
                     PDTATEGGLVALRVLAGPNDTLQIGLQYDAVNNRPTTVSGFPEAAFAAAPSLPGVLKA
                     DRGTVTAVALAAERDQKLAALAEGRKVRQALIEKSFEEAQSRENPGKWSLGLSAEDFA
                     ASYDGTDADIPAAVRAALKERVGQERDRRAAREKLWDAEAAKALAAAFQGSSLPLTAE
                     RKAGFTAKKADWTASTGRDTLRDWAGRYEQELSTLWRRGKETMERESRAALAAALAAR
                     GESPNDAEYYESAFAPVLQKLEPGADPSETVKKAAVDFLAAHEAEQKAAREKDRQDRI
                     AKGLKGSSDALRKALTDIGAEWLWTSWQRNDQIHAAFQDVVRQHINDPGARLDSLASR
                     FVREQQEKSTAGKNPPAATIRGLTPLIGRLYGLNPDVPADLQRIVNEKAPIVETVLTG
                     VLYFAGVLLISQAGPLFGLALDPSVFNAYSLFALVNLAFGAAHARVYRFVPPARGDRR
                     LGRWELDRRPDGSPQQSSLGTRLNLAIRGLVIHSPLLLAFMDPMGGLFLAPLGAVASV
                     ALHLAHNRLAARLGWTAAAAAPSGEQGPPSNAKRLLKMSLADFLQEYAEEDFVRPGYF
                     GYNAPAIRQRLDEWFGPNGWSIKHIVRGRIVEESEAYALYEEGYYQYFKSHPEELNRL
                     VRMARDVYDTDPSNVASGGDYTKQGDTAARHLQDIAIRNVVKRLGRTFEGTELIQVRG
                     RGTPGEQWAPGNIPIHDASLIRQPEMRNWWKAGSVESFFQSTKIIVLNPHPVAERLFE
                     NHRRFFGKGRLFQLKDLAALPWALDADQFPKDADGRP"
BASE COUNT         1309 a         2076 c         2487 g         1370 t
ORIGIN      
        1 gggggcgtcc gtcggcgtct ttggggaatt ggtccgcgtc cagcgcccac gggagagcgg
       61 ccagatcttt taattgaaaa agccgtcctt ttccaaaaaa ccgccggtga ttttcgaaca
      121 gacgttcggc caccgggtgg ggattaagca caatgatctt ggtggattgg aaaaagctct
      181 cgacgcttcc ggctttccac caattccgca tttccggttg ccggatcagc gacgcgtcgt
      241 ggatgggaat atttccgggg gcccattgtt cgcccggggt tccccggccc cgaacctgga
      301 tcaattcggt gccttcaaag gtccgtccca accgcttgac gacgttgcgg atggcgatgt
      361 cctgaaggtg gcgggccgcc gtgtcgcctt gtttcgtgta atcgccgccg ctggccacat
      421 tcgaggggtc ggtgtcgtag acgtcccgag ccatgcgaac caggcggttc agctcctcgg
      481 ggtgggactt gaaatactgg tagtaccctt cctcgtacaa cgcgtaggct tccgactctt
      541 cgacaatgcg gccgcggacg atgtgtttga tcgaccatcc gttgggaccg aaccattcgt
      601 ccagccgttg gcggatggcg ggcgcgttgt agccgaaata cccggggcgg acaaagtcct
      661 cttcggcgta ttcctgcagg aaatcggcca aagacatttt aagcaggcgc ttcgcgttgc
      721 tcgggggacc ctgctcgccg ctgggagccg cggcggcggc cgtccagccg agccgggcgg
      781 cgaggcgatt gtgggccaaa tggagagcga cgctggccac cgcgcccaga ggcgccagga
      841 agagcccccc catcggatcc atgaaggcca gaaggagagg ggaatggatc acaagccctc
      901 ggatcgcgag attgaggcgg gtgccgaggg aggattgttg gggagatccg tccgggcgac
      961 gatcaagttc ccatcgcccc agacgccggt cgccccgggc cggaggcaca aaacggtaaa
     1021 cccgggcgtg ggcggcgcca aaggccagat tgaccaaggc aaacagggaa taggcgttga
     1081 acaccgaggg gtccagcgcc aggccgaaca gcgggcccgc ctgggaaatc aagaggacgc
     1141 cggcgaagta aaggactccc gtcaaaacgg tttcgacgat cggggctttt tcgttgacga
     1201 tccgttggag atccgcgggg acatcggggt tcaaaccgta gagccggccg atcaacgggg
     1261 tcaacccgcg aatcgtcgcg gcggggggat tttttccggc cgttgatttt tcttgttgct
     1321 cccgaacaaa acgggacgcc agggagtcca gacgcgctcc cgggtcgttg atgtgttgcc
     1381 gtacgacgtc ctgaaatgcg gcgtgaattt ggtcgttgcg ctgccacgag gtccagagcc
     1441 attcggcgcc gatgtccgtg agcgcctttc gaagggcgtc ggaggagcct ttcaaaccct
     1501 tggcgatccg gtcttggcgg tctttttccc gggcggcctt ttgctcggct tcgtgagccg
     1561 cgaggaaatc gacggcggct tttttcaccg tttcggaagg atccgccccc ggctccagtt
     1621 tctgaagcac cggggcgaag gcggattcgt agtactcggc gtcgttcggg gattcgcccc
     1681 gggcggcgag cgcggcggcc agggccgcgc ggctttcacg ttccatcgtt tctttgccgc
     1741 ggcgccaaag ggtggaaagt tcctgttcat accgaccggc ccaatcccgc agggtgtcgc
     1801 gccccgtgga ggccgtccaa tcggcttttt tcgcggtgaa tccggctttc cgttcggccg
     1861 tcagggggag cgaagaccct tggaaggccg ccgccaaagc tttggccgcc tccgcgtccc
     1921 agagtttttc ccgggccgcg cggcgatccc gttcctggcc cacgcgttcc ttcaacgcgg
     1981 cccgtaccgc ggcggggatg tccgcgtcgg tcccgtcgta ggaggcggca aaatcctccg
     2041 ccgacagtcc gagcgaccat tttccggggt tctcccgact ttgggcttct tcgaaacttt
     2101 tctcgatcag ggcctgacgg actttccggc cctcggccaa agcggccaac ttttgatcgc
     2161 gttcggcggc gagagcgacg gccgtcaccg tgccccggtc ggccttcagg accccgggga
     2221 gggagggggc ggcggcgaaa gcggcttcgg ggaaacccga aaccgtcgtg ggacgattat
     2281 taacagcgtc gtattgaagc ccgatttgaa gagtgtcgtt cggccccgcc aggacccgaa
     2341 gggccaccag gccgccctcg gtggcggtgt cgggggaggt caacccgacg cctttttcgg
     2401 tgaaaaagag ttcggacccg agtccgaggc tctccgtggg atcaatcccc ttttcgaaaa
     2461 gcgcccgggc cacgacgatc ggttgagcgt ccagggccgt caattgattg tccgtgtgct
     2521 taaaccgttg ggcgtgaagg accccgccgg acagccacac tttgacggcg agattgcctt
     2581 ggccgaccca ggccgcgccc aacagagggg cggtcaggag ggaatcggtc cgctcacgtt
     2641 tctcgacccc gcgggggccc agggtgaatt gattttcaaa aggttggagc ttgccgccgg
     2701 gttttcgcac ctcgcccagc cgcggcaagg tgaattccgc caccgcgggc aggcgataac
     2761 gcttcaggaa cggcgggagg aaatcgcgtt gatcggaaac cttcaacgga tcggtccgag
     2821 ggcggtgaaa cgccaaattc agggggagat cgccgatgag tccctggcgg gcgggggtca
     2881 ccttttcgcc gtccttggtt tgggttttca ccgcttcgta gccgaggaaa tcggccaggc
     2941 ggtccaggac cccccgcgcg gattcggcgt cgacgcggga agtgacctcc cggcgcgccg
     3001 gccccgggac gccctgccgg gtcaggatgt ccagaatcaa ttcgcgggac gtcgggtaac
     3061 gaagaacggg ctcggggcgg atgtcgattt tcttctcccc gttggcgacg agcaattcgg
     3121 ccaaggaatc gtatccgaga agttgcgtca gcgcttgggc cacggcgaat tcccgctcca
     3181 gcgattcggt ttcctccggg ttcggttggg atccttcggg gatcgtccct tggtggaagg
     3241 ccaaaaggta atgaaggtcc gtggtgtatc ccaggccgtc catcgcgaga tccacgcgcc
     3301 gccggtcggt gttggcccgc atgtaaacca ggtcggcggt gggcggggtg gttttctgat
     3361 caaccccgcc cgcggtcagc gcggaccaac cgcgttcggc ggccaatcga ttgtgaacgg
     3421 cgtggagacc cacgctggtc agcgccgcga tcggcgccag gaagagtccg cccagaggat
     3481 ccatgaaggc caacaagagg ggcgcgtgaa tggcggcgcc ccggagcgcc aagccccatc
     3541 gctcccgacg ggacgccggg gccggctcgg cttcccagcg gcccaggcgg ttgacgcccg
     3601 gaacgggggg cttgaagcga tagaccgagc ggtgcgaaaa gccgaaggcg agattcacga
     3661 gggcgaagag gcccgtggcg gcaaaaaaag cgtggtcgat gtggaggccg acagcggaac
     3721 ccagggcgct cagcaggccc accgcggcgt agtagaaggc gccggtgacg acggtttcaa
     3781 caaggggggc tttttcgttg acgatgcggt tcaaatccac cggattggcg ggattgagtc
     3841 cgtaaagccg gccaatcaat gggaccaatc ccgaaattgt cgccgccgga ggcccccggg
     3901 gcgccacggt ggacgaaggc acgagggtgc cgttggtgga atcgttgtcc agaatcacca
     3961 cgtcgctgcc caccagggac agggtgaagt gcttggcgga aataccggaa accgcgccga
     4021 gccccaaatt tccacggccg aattcaatgg tggagcccgt taagcgctct ccggatggca
     4081 gaaggagagt gatttttccg ttggccaccg attcgaccac caggtgcgtg tctttcgcaa
     4141 cgatggggaa cgggaggttc ttttgcggca cgctcatgga tagcttcggg aatttggatt
     4201 ggccgcccaa taaaacggtt tcctgcacgt ttcgccaggc gttgatcagc gccgagaaat
     4261 actccaaatt gtaaggaatc ggccgacgcg tcgtttggtt ggttaggagg gtttcggcgt
     4321 tggctcccag ggaatacacg atgttttcga tgtccgcgcg tccccgcggt ttggccaggg
     4381 ccgccagggc ggtcgccacg tcttcggtca ctcgcgagaa cgcggcgccg acggtgccga
     4441 attccgctga aaagtccaac gggcgaacct gaccgaccgc gagggattgc aacgtttgaa
     4501 ttttggatcc caggccgtcc cgaacgcgtc cgatggtcgc gtcgacggtg tgtccgtccg
     4561 ccgaccgcgc gaaaacggtg atttcgtggg gttcgaagcc ctggaggtcg cgggccggga
     4621 tggcttcgtc ctcccggaac acgcgggcgg cctccccatt ggtgtaaacg gataaattga
     4681 ctttgacggt ttgcccgtcc atcacggcgg ggatctccga ttggagagcg gcccgaagct
     4741 tcgcgtcgaa ctcgtcgccc aggggatcgc ccggcttgac gtaggtgccg ttgggggaca
     4801 aatcggccac gagggcggtt tgaccgtccc attgcaccgt ggcgtggcga cgggagacat
     4861 ttttgacgtc ctgggcttta atgaccaggc ccaccgaatt ttcgtcgggg gagcggccga
     4921 tggagagggt ttggccgggt tgaagcacgg cggtcgagcc gtcgggcccg gtggccacca
     4981 gattcccgtg atcgattcgc agggaaacgg ggtgtacgtt ggcgagcaac aggcgcgagg
     5041 ggaattgagg gagccggtgc gggccggatt cgggcgtcaa cagggtcggg ccggtgattt
     5101 ccggaagggc ggcggggggc gtcgttgtgg gcggcgtgac gccgaggacc aggcgttgaa
     5161 ggggttccat tttcaactgc caaggcgggc tgatgaagga tccgtcggtc cgggtgaagt
     5221 caaagagagc gttgagggac gtggggccgt aattcaggcg cccgttttcc gcgtaatagg
     5281 cgtcggtggt gtaggcgccg gcgtccgatt gataataggc ggtcaggagc tcccaatagg
     5341 tttcgacgtc caattccaag cgacgggcgt tggccgaaat ttcggcggcc gccttgtagg
     5401 ccaggggcga aggttccgtt ccttgggcga gagcaataaa aagcgggtcc cgggccgaga
     5461 ggaactcggt gctggtgggg ggattcgcgc gaaggtattg accgatgggg tcgccgccgg
     5521 ccagggacaa ggccaaatcc aattgcttgg aggacaatcc gtgccgctcg aaatagcgcc
     5581 gaatcaatcg cgaggtgtgg tcgaattcgt tctccccgac cctcccggcc cgggcctcgg
     5641 gtttgccgat gtcgtggagg gccatgaaga ggcggaaaaa accgagatcg acgcccgctt
     5701 tggtcaacgc ggcttgaaag cggggcgaag cggcgaagta tcgctcgaat tgccggagga
     5761 ccatggcggt gtgttgataa agacgatggc cttcgtcggt ttccgtgccc atcaggaaaa
     5821 tccggccaag ttcccggtcg ttcatcagct cggccacgaa tttttcgggg acgaattcgc
     5881 cgacgggaag gcccatctga tggaccacca ttaaaggaag gggttggacg gtgctgacca
     5941 cgagaggtcc ggattcttcc gtcgcgggcc cctcgttcgt cacgcggccg gatcggttga
     6001 gaagatggtt ggccagttgg aggtcgttga agaaaccgcg aacccgctcg gtttgccatc
     6061 ggccggtttc atcggttcgg acgttgagct ggatcatgcg gtgttggact tcctccacgg
     6121 aagcccgccc gtccggatgg gattcgtcgc ccggcaatac catcgcgttg gtttccggcg
     6181 agaaacgcca gccgaattgg gtgctgccga ccaccacgcg ttcgacggcc gttttgtcct
     6241 cgttcaaaaa gaccgcgacc gtgtgttccc cggcggtttt ttggaggtcg cccggcaaat
     6301 cgcggtggag ggcttcgctg atgtggcgcg tgaggcgcca ctgaaaaaag tcgaaatctt
     6361 tggggaacac ttcgtcgtaa ccggtttcgg ggtggatgtt gcgctggaag tccaattcaa
     6421 accccgggtt ccattccagg gaaaaggcgt ggaccccgtt caagggcatg ttggtttcgg
     6481 tgaaatgggc cacgaggtcc ccgagggagt cctgttcgac ggattcaagc gggttctggg
     6541 acacgaggtc gtgaagggtg gtcacccaga tttggccctt gggggaggcc aaatagcggc
     6601 ggacgttgtg ggacaaatag cggttcacgt cggggtcggc gagattaccg gccttcagcg
     6661 cttcgacgat tttgtccttg gccgcgagcg gcacggcggc ccaccaggtt cgaccgctgt
     6721 gctgtccgga acgcggcccg tcgatttccc ccgaaaagcc ccgaataagt ccgtagtttt
     6781 cctggttcaa cgcccaggtt tgaatggcct tggaccgttc gagcaagtct cgatcgtaga
     6841 tgggatcgta gttgattttg tagccgatgg tcggttcgcg gctcccggtg atgtggttgg
     6901 tgtcggccaa ggtaatcggt ttgcccgccg gaccgacctc gacgatggtg ttggtgggtt
     6961 tcaaccccat gagatcggtc gccagagaca cgaggtttcc gaaaaacccc cgccccttgg
     7021 aatcgttgcc cgtcaaaaag gcgcggcctt cgcgtcggcg ggccaaattg accccggagt
     7081 ggcccaaaag cccggcggtg aaaccgccga ccaaggggcc caggaccgcg gcgctcgcgc
     7141 ccgcgagttc ccgcaggctt tgaagggggg tcgcccagaa actggaggtc cccacggaaa
     7201 gcacggaatc cgccagcccg cgcgccagga cggggaggtt tt
//
LOCUS       DAOQLS010000011         7209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_4280_length_7209_cov_4.362035, whole genome shotgun sequence.
ACCESSION   DAOQLS010000011 DAOQLS010000000
VERSION     DAOQLS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4280_length_7209_cov_4.362035"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3788)
                     /locus_tag="PK362_00395"
     CDS             complement(<1..3788)
                     /locus_tag="PK362_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNC73333.1"
                     /translation="MSIKRAMGWLAGIGLALGAAPATAAVSISGSVVQSSTISATPSG
                     VALMVSTFPVTDPALANDPSVFIASAPLSVIGSGPFSFPALPFFGNPTEYYLYAYRDL
                     NGDGVPGSTEPLGGYGPFPFNDPAAHWTAPVDSVSTSGVTVNLIPRAEVTGQLFNNSG
                     QSGQRLIVRAIDLAHPPHKWQTIEFPVGGGPFPYSLGGLVPTLNYTIEAWIDTQPKGS
                     TGAYNPEPFEDKAPSAGPYAAFMGITTPNVDLTISSGALGGSTPDHIRLSGLNDSWGM
                     TIASGVPSGDLTVSVRDFNDALTATGAPTALIFSGFDSGGGFSPELSTDAFATLFTTA
                     TLPGGQPFSGKFKFRYPGQGNVLLQAEAVNFPTAGECRYAFFSFNVLPGGAAFANLNA
                     RTVSDPATTTGTTTSITPDRDGVDDAAVLSCDPPDPNVYWECDLSTEPTFTTGILERF
                     FGNGPGEVTWYGSAFDGRTVPNGTYFARFQTEGAGLVSSTLTVSVNTAFVRGTVTDAV
                     FSPVADANVQINGPGGGGFARTANDGSFFVGGLTAGQTYNLDVSRPGFAPQSFAVAAS
                     SDVGDVVLGPGATLRVKATVDTAPPFDLYGNIFVHDASYSSVAGGPLHLAPGNTVSDN
                     GLPAADPNFSNFTSMAVKPGVAYTVEINLPQYGHFTIPGQSTGPGQTVDVVQAMFRKA
                     NVAGALNFPAALSSPFGGEWISVDAVPTAPVGAPPAAWGGAFVPNGASSATFQMFGVS
                     AGTYTLRAFARGFIQNSTTPVVVGNTDLSGVDFPPFSTGGTATGTITINGDTSGLAGG
                     NFGGAACGAGQVPLPVHAFDPGSFVSAFTQVCVSTGPGPVTSALYQMNGLPDGTYELS
                     AFLPGFQINPPGPKFVTVSGGVGNTDLTFDAFTGRLDIVAALPPGDLAGAVAYRLERD
                     GPDRDERSGNLAGPVGGPATGSELALGTGLYTLTLLNGNPGRGLIRRIGVSVKNGSTT
                     TATVDMAIPTYAVGGAVSLQGNLILPSTWSVTVSSMAGLTAAAVAPTVQVFAFPLPNH
                     FDDRTKPLREIPVTPWAASGTFALNGLTPGGYLFRIKEDLNPPSPPANCNGCSAPPGL
                     PEMASSDRVVFLTTAPVTGADLTLSNGAKLTGTLRRPVGDDSTDPRHFVLRLRRTDNL
                     TVYETETDTNGTGAASYTIPHLAAGIYILEVYEKTPPVKYGAPAQTVEIANGDAALDV
                     ELVAAATLVGRLRDADTGTLITPANAGRFLPDRFEVFAEAKPFVPGGYANAERNENGP
                     G"
     gene            complement(3778..4977)
                     /locus_tag="PK362_00400"
     CDS             complement(3778..4977)
                     /locus_tag="PK362_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73334.1"
                     /translation="MRRPAAWAAVLLLTGTAGAAEVTPLWALQFMGGQHFFEGRSSAL
                     AGNIDVHYTPAVKFSDRWTLLPTYAGSYSGTRDVQELAGGGTLFQDSMSHGLLVKSVH
                     ALGERWKLKPSAGAKLELLRETVDEQWGDGLFDYRKWSGGLEAEFDQSKNAGGRFAYD
                     FYQLTFPNYESLESAADPTLARELAGAKVLDSSNHLITLGWWSPLPAAGRLEAAAFIN
                     RRSFGDQPVVKSDGGLTGTERADTTLSLHVETAYPFTVGDAARAVGTLGLSYGSQSSN
                     QNHFDARKAVFLPNYYDYSQWSLSPQVSAAFGESKWVSSLSASYQRRTYSNRPTQDGN
                     GDYLSEKLSLSEITTQFAVTYPVAKNMRLRFQTSLAWSDSNMQYEKVFRYNYKIANYM
                     MGFSYEY"
     gene            complement(4974..6680)
                     /locus_tag="PK362_00405"
     CDS             complement(4974..6680)
                     /locus_tag="PK362_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNC73335.1"
                     /translation="MGLSNAQRRVGLWTLGALLPLVFLHSLWADPVVVNAVRGSAEIR
                     RARAQNNKWYALRKGSPVKPGDEIRTGKSSRAVLKLDDGSRVVLSGGSRAKVLESSPN
                     RIFALAVGRMKSFVKKLKPTSKFEVRTPLAAASVRGTIFEMGVGEDGKDGYLDVSRGV
                     VNLAQGGRDLDVHAGERVDFMSGWPLGDPVPGKSSGNAPADEEKTDDKSALKREVGLG
                     MSKEAVMAAAAEEMRLAEYQEGKTLIDVNGDRVRLEEYIIRRPQELAANLQDRAFKLV
                     VLNERDNRFDYFYYRGVFNQALPDDLSVALNGVRGQLGAAAPDYYLDSYEMGQSNTRD
                     SVLDQATGGHLVKITYDGTNYYLEDPTDASNTRTILADEVTTTVDGTFHKVYDPVGDR
                     FVTITDAQFTAGGGAAGVYDASQDTFRTLTSADTYWRTAFNNYSHLINGNSKQSYVPD
                     GSLGITNILAQDQDALYTFAGGSVFPVTETPSGSDVLHNRITLYYGDGTTETVNTYIM
                     SDDGKIGPVSAFDGLTTGSAFKDQLLQWNYEQVTEATEFQGRKIDLVVEPKILIKSGL
                     LK"
     gene            complement(6747..>7209)
                     /locus_tag="PK362_00410"
     CDS             complement(6747..>7209)
                     /locus_tag="PK362_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC73336.1"
                     /translation="DVAARAGKPVVLHCRDAAAPSAAAEADLLEILRRRPDRLSDGGP
                     AGVAHCFQGSASAAWELLRQGFLIGVDGPLTYPNASPLRALIGHLPLESLVLETDSPY
                     LPPQSHRGKRNEPALLPAVVRALAELKHLAVEKIAAATAANARRLYRLPPL"
BASE COUNT         1326 a         2176 c         2413 g         1294 t
ORIGIN      
        1 ccgggtccgt tttcgtttcg ttcggcgttg gcgtacccgc ccggaacaaa cggtttcgct
       61 tccgcgaaca cttcaaagcg atcgggcaag aaccgcccgg cgttggcggg cgtgataagg
      121 gtccccgtgt cggcgtcgcg aagacgcccc accagcgtgg ccgccgccac caattcgacg
      181 tccagggccg cgtcgccgtt ggcgatttcc accgtttgcg cgggagcgcc gtatttgacc
      241 ggcggggttt tttcgtacac ctccaaaata taaatcccgg ccgccaggtg ggggatggtg
      301 taggacgccg cgccggtccc gttcgtgtcg gtttcggttt cgtaaaccgt gagattgtcg
      361 gtccggcgca aacggagcac aaaatgccgc gggtccgtgg aatcgtcgcc gaccggtcgg
      421 cgcaaggtcc cggtcaattt ggccccgttg gacaaggtca aatcggcgcc cgttaccggc
      481 gccgtggtga gaaacaccac gcggtccgaa ctggccattt ccggcaggcc gggcggcgcc
      541 gagcaaccgt tgcaattggc cgggggcgac ggcgggttga ggtcttcctt gattcggaaa
      601 agataaccgc cgggcgtcaa gccgttgagg gcgaaggtgc cggaggcggc ccagggagtg
      661 accgggattt cacggagagg tttcgtccga tcgtcaaaat gattgggcaa ggggaaagcg
      721 aaaacctgca cggtgggagc caccgcggcg gcggtgagtc cggccatgga gctgaccgtc
      781 acgctccagg tgctcggcaa aatgaggttc ccttggagcg acacggcccc acccaccgcg
      841 taggtcggta tcgccatgtc gacggtcgcc gtggtcgtgg acccgttttt gacggagacg
      901 ccgatccggc ggatgagccc ccggcccggg ttcccattga gaagcgtgag ggtgtagaga
      961 cccgtgccga gagcgagttc cgatcccgtg gccgggccgc ccaccgggcc ggccaaattc
     1021 cccgatcgtt cgtcccggtc cggcccgtcc cgctcgagcc ggtacgcgac ggcgccggcg
     1081 aggtcccccg gaggaagggc tgcgacgatg tcgagtcgtc cggtgaaggc gtcaaaggtc
     1141 aaatccgtgt tcccgacccc cccggagacc gtcacgaact tggggccggg cgggttgatt
     1201 tggaaccccg gaagaaaggc ggacagttcg taggtgccgt ccggcaaacc gttcatttga
     1261 tagagggcgg aggtgacggg acccggaccg gtcgaaacgc acacctgggt gaaggcgctg
     1321 acaaaacttc ccgggtcgaa agcgtggacc ggcaggggaa cctgtcccgc gccgcaggcg
     1381 gcgccgccga agttcccccc cgccagaccc gacgtgtccc cgttgatggt gatcgtgccg
     1441 gtggccgttc cgcccgtgct gaacggaggg aaatcgaccc cggaaaggtc ggtgttcccg
     1501 accaccaccg gggtcgttga attttggatg aacccccggg caaaggcccg aagggtgtag
     1561 gtgcccgcgc tcaccccgaa catttgaaag gtggccgagg acgctccgtt cggcacgaac
     1621 gccccgcccc aggccgcggg gggcgccccg accggcgcgg tgggcaccgc gtcgaccgaa
     1681 atccattctc cgccgaaggg gctggaaagc gccgcgggaa aattgagcgc cccggccacg
     1741 ttggctttgc gaaacatggc ctgcaccacg tccacggttt gccccggtcc ggtggactgc
     1801 ccgggaatcg tgaaatggcc gtattgggga agattgattt cgacggtgta ggccacgccc
     1861 ggcttaacgg ccatggaagt gaaattgctg aagttgggat cggcggcggg caacccgttg
     1921 tccgaaacgg tatttcccgg ggccaaatgc agggggccgc ccgcgacgct tgaataagag
     1981 gcgtcgtgga caaagatgtt tccatacaaa tcaaagggcg gcgcggtgtc caccgtggcc
     2041 ttgacgcgga gggtggcgcc cggaccgagg accacgtcgc ccacatcgga actggccgcc
     2101 acggcgaaac tttgaggggc gaacccgggc cgggacacgt ccaggttgta cgtctgcccc
     2161 gcggtcaatc cccccacgaa aaaggaaccg tcgttcgcgg tccgggcaaa cccgccgccg
     2221 ccgggaccgt tgatttgaac gttcgcgtcg gccacggggc tgaagaccgc gtccgtcacg
     2281 gtgccgcgca caaaagccgt gttgaccgag acggtcaagg tggaggaaac caaccccgcc
     2341 ccttcggttt ggaatcgggc gaaataggtt ccgttcggca cggtacgccc gtcaaacgcc
     2401 gaaccgtacc aagtgacctc cccgggcccg ttcccgaaaa accgctccag gatgcccgtc
     2461 gtaaaggtgg gttccgtgga cagatcgcac tcccaataaa cattcgggtc gggcggatcg
     2521 cacgacagaa cggccgcgtc gtccaccccg tctcgatccg gggtgatgga ggtggtcgtt
     2581 ccggtggtgg tcgccgggtc ggacacggtt cgcgcattca aattggcaaa ggcggctccg
     2641 ccgggaagga cgttgaagga gaaaaaggcg taacggcact cgccggcggt cggaaaattg
     2701 acggcttcgg cctggagcag gacgttcccc tggcccggat accgaaattt gaattttccg
     2761 gaaaagggct ggccgccggg caatgtggcg gtcgtaaaga gggtggcaaa ggcgtcggtg
     2821 gacaattccg gggaaaagcc cccccccgaa tcgaatcccg aaaaaatcaa cgccgtggga
     2881 gcgcccgtcg ccgtcagggc gtcgttaaaa tcccgaacgc tgacggtcaa gtcccccgat
     2941 ggaacgcccg aggcgatggt catgccccag ctgtcgttca acccggaaag ccggatgtgg
     3001 tccggggtgc ttccgcccaa cgcgccggaa ctgatggtta aatcgacgtt cggcgtcgtg
     3061 attcccatga acgcggcgta gggacccgcg gacggcgctt tgtcttcaaa gggttcgggg
     3121 ttgtaggccc ccgtggagcc cttcggttgg gtatcgatcc aagcttcgat ggtgtagttc
     3181 agggtcggca cgaggccgcc cagagaataa gggaacgggc cgccgcccac cgggaattcg
     3241 atcgtctgcc acttgtgggg cgggtgggcc aaatcgatgg cccggacaat caaacgctgt
     3301 ccggattggc cgctgttgtt gaagagttgg cccgtcacct cggcccttgg aatcaaattg
     3361 acggtgacac ccgacgtgct gacgctgtcc accggcgccg tccaatgggc cgccggatcg
     3421 ttgaagggga aaggaccgta cccgcccagg ggttccgtgc tgcccgggac accgtcgccg
     3481 ttgagatcgc ggtaggcgta gaggtaatat tccgtcgggt taccgaaaaa gggcaaggcg
     3541 ggaaaggaaa acggtccgct gccgatcacg gacagggggg cgctggcgat gaacaccgac
     3601 gggtcgttgg cgagcgccgg gtccgtcacg gggaaggtcg acaccatcag ggcgacgccc
     3661 gacggcgtcg cggagatggt ggagctttga acgacgctgc ccgagatgga aaccgccgcc
     3721 gtggccgggg ccgcgcccag ggccaacccg atccccgcca accatcccat cgcccgctta
     3781 atactcataa ctgaacccca tcatgtagtt ggcgattttg tagttgtagc ggaaaacctt
     3841 ctcgtattgc atgtttgaat cgctccaggc cagggaggtt tgaaagcgaa gccgcatgtt
     3901 tttggccacg gggtaggtca cggcgaattg ggtcgtgatc tccgagagac tcagcttttc
     3961 cgataaataa tcgccgttgc cgtcctgggt gggccggttg gaataggttc gccgttgata
     4021 ggacgccgac agggaactca cccatttgga ttcgccgaag gcggcggaca cctggggcga
     4081 caggctccat tggctgtaat cgtaatagtt gggcaaaaac acggcttttc gggcgtcaaa
     4141 gtgattttgg ttggaagact ggcttccgta ggacaagccc agcgttccca ccgcccgggc
     4201 ggcgtcgccc acggtgaagg ggtaggcggt ttcgacgtgg agcgacaacg tcgtatcggc
     4261 gcgttcggtc ccggtcagtc cgccgtcgga tttgacgacg ggttgatccc cgaaagatcg
     4321 ccgattgatg aaagcggccg cctccaggcg gccggccgcg ggcaggggcg accaccaacc
     4381 cagggtgatc aggtgattgg agctgtccaa caccttggcg ccggccagtt cccgggccag
     4441 ggtcgggtcc gccgccgact ccagagattc gtagttggga aacgtcagtt gataaaaatc
     4501 gtaggcgaat cgtccgccgg cgtttttgct ttgatcgaac tccgcttcca ggccgccgga
     4561 ccatttgcgg taatcgaaga ggccgtcgcc ccattgttcg tcgacggttt cccgaaggag
     4621 ttccagcttg gcgcccgccg agggtttcaa tttccagcgt tcgcccaagg cgtgcacgct
     4681 tttaaccagc agaccgtggc tcatggaatc ctgaaacagg gtgccgccgc ccgccagttc
     4741 ctggacgtcc cgggttcccg aataacttcc ggcgtaggtg ggcagcaggg tccatcgatc
     4801 gctgaatttg acggccggcg tgtagtggac gtcgatgttg ccggccaggg cgctggaacg
     4861 tccttcaaaa aagtgttgtc cgcccatgaa ttgcagggcc cagagcggag tgacttccgc
     4921 cgccccggcg gtgccggtca acaacaggac cgcggcccaa gccgccggac gcctcatttg
     4981 agcaaccccg acttgattag aattttgggt tccaccacca aatcgatttt gcgcccctgg
     5041 aactcggtcg cttcggtcac ctgctcgtag ttccactgaa gcaattggtc cttgaacgcc
     5101 gaacccgtgg tcaatccgtc aaaggcgctc acggggccga ttttcccgtc gtcggacatg
     5161 atgtaggtat tgacggtttc ggtggtgccg tccccgtaat acagggtgat ccggttgtga
     5221 agaacgtcgg agccggaggg cgtttccgtc acggggaata cgcttccgcc cgcaaaggtg
     5281 tagagcgcgt cctggtcctg ggccaaaatg ttcgtgatgc ccagggagcc gtcgggcacg
     5341 taggattgtt tgctgttgcc gttgatgaga tggctgtaat tgttgaaggc cgtgcgccaa
     5401 taggtgtcgg cgctcgtgag cgtgcggaag gtgtcctggg acgcgtcgta caccccggcg
     5461 gcaccgccgc cggcggtgaa ctgggcgtcg gtgatcgtga cgaaccggtc gcccacgggg
     5521 tcgtaaactt tgtgaaacgt gccgtcgacc gttgtcgtga cttcgtcggc caaaatcgtc
     5581 cgggtgttgg aggcgtcggt ggggtcctcc aaataataat tggtcccatc gtaggtgatt
     5641 ttgaccaggt ggccgccggt ggcctggtcc aaaaccgaat cccgcgtgtt ggattggccc
     5701 atttcgtagg aatccaaata gtagtcgggg gcggcggcgc ccagttggcc gcgaacgccg
     5761 ttcagggcca ccgagaggtc gtccggcagc gcttggttga agacgccccg gtaataaaaa
     5821 taatcgaagc gattgtcccg ctcgttcaga acgaccagct taaaggcgcg gtcctgaaga
     5881 ttggcggcca gttcctgcgg gcggcggata atgtattcct ccagccgcac ccggtcgccg
     5941 ttgacgtcaa tgagcgtctt gccctcctgg tattcggcga ggcgcatttc ctccgccgcc
     6001 gcggccatga cggcctcttt gctcatgccc agccccactt cgcgcttcaa agccgacttg
     6061 tcatcggttt tttcctcgtc ggcgggcgcg ttcccggagg atttgccggg caccggatcg
     6121 cccaggggcc aaccgctcat gaaatccacc cgctcgccgg cgtgcacgtc caggtcccgc
     6181 ccgccctggg ccaggttgac cacgccccgg gacacgtcca agtagccgtc cttgccgtct
     6241 tcgcccacgc ccatttcaaa aattgttccg cgcacggagg ccgccgccag gggcgtgcga
     6301 acttcgaact tgctggtggg tttgagtttt ttgacgaagg acttcatccg gccgacggcc
     6361 agggcgaaaa tgcggttggg ggaagattcc aagactttgg cgcggctgcc gccgctcaac
     6421 accacccggg aaccgtcgtc caactttagg accgcccggc tggatttgcc cgttcgaatc
     6481 tcgtcgccgg gtttgacggg ggagcccttc cgaagcgcgt accatttatt gttttgggcc
     6541 cgggcccggc ggatttccgc cgatccccgc acggcgttga cgaccacggg gtccgcccac
     6601 agggaatgca aaaacaccag gggcaacaaa gcccccagcg tccataaacc cacccgacgt
     6661 tgcgcgttcg acaaacccat ttggcaggga cccgtggagg tccatggaag tgtaggaccc
     6721 gaacccgctt ttgtcaacgg cgcgtattaa aggggcggga ggcggtagag tcggcgggcg
     6781 ttggcggcgg tggcggcggc gattttttcc acggccaggt gcttgagctc cgccagggcc
     6841 cggaccacgg ccgggagcag agccggttcg ttgcgtttcc cccggtggga ttggggcggc
     6901 agatagggac tgtccgtttc cagcaccagg gattccagag gcaggtggcc gatgagggcc
     6961 cgcagaggag aggcgttggg gtaggtgagc ggaccgtcca cgccgatcag aaacccctgg
     7021 cgaaggagct cccaggcggc ggaggcgctg ccttgaaaac aatgggccac gcccgccggt
     7081 cccccgtcgg acagtcggtc gggccgccgg cgaaggatct ccagcaagtc cgcttcagcg
     7141 gccgccgaag gggccgcggc gtcccggcaa tggagaacga cgggttttcc ggcccgggcc
     7201 gccacgtcc
//
LOCUS       DAOQLS010000012         7171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_4307_length_7171_cov_4.097667, whole genome shotgun sequence.
ACCESSION   DAOQLS010000012 DAOQLS010000000
VERSION     DAOQLS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4307_length_7171_cov_4.097667"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /gene="mutL"
                     /locus_tag="PK362_00415"
     CDS             complement(<1..581)
                     /gene="mutL"
                     /locus_tag="PK362_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNC73337.1"
                     /translation="MKNKVRKLPDDIVRGIAAGEVVERPASVLKELLENAVDAGARRI
                     EIEWRDAGRRRLRVTDDGGGMTPDDARLALERHATSKISELTDLETLSTYGFRGEALP
                     SIAAVSRFGLSTRSAEGEGWSIALEGGRFLREGPAGVPPGTTITVDDLFFAVPARLKF
                     LKSDATERGLLLRTVEDVALAEPGLELRILSEDR"
     gene            complement(578..1012)
                     /locus_tag="PK362_00420"
     CDS             complement(578..1012)
                     /locus_tag="PK362_00420"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC73338.1"
                     /translation="MKPDNRRHKRFPVLKDMAEPVDLFVMDQPPREVPAVLTNLSAGG
                     MALVVFAHVSGDAKLKILLDVHGLENLELQGRVAWTEVKGDTTAIGVRFDHLSHDTVH
                     RITKMAEDFQDCELKISFGLKDVCFRQCAYWTLCGKPVKLKH"
     gene            complement(1095..3689)
                     /gene="mutS"
                     /locus_tag="PK362_00425"
     CDS             complement(1095..3689)
                     /gene="mutS"
                     /locus_tag="PK362_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNC73339.1"
                     /translation="MSETTAAATPIMRQYQALKAQNPDAILFFRLGDFYELFDEDARR
                     AAPILELVLTQRQGIPMCGLPHHALPGYLGKLLKRGWRVAIAEQMEDPATTKGMVRRD
                     VVRVVTPGTVLEEELLPAKQNNFLAAAWPGRGGSPWGLAWADMSTGELSFASLPDGGA
                     VADELSRWDPRETLWPAGGPDPDGDRAVTRVEPSTFLPDSALRRAPALFGVKSFEGYG
                     LSADHPALPAVAALLNYIERNQAPALGALRPPRAHAPTRFMALDALALRRLDLFPSDS
                     GGDPQRPTSLWHLLDHTATAMGGRRLKGWMVNPLMDLEEIAARQDRVDYFLHHGRLRR
                     AVVDLLRETADVERVLSRLTAGTASGRDLNALRRTLRQAPDLERTLRSGGDPLAGQHP
                     LGSLTRSFQVPPGPGELLEKALAEDPPQRLQDGGVIRDGYSAPLDEWRQLARNGRGWI
                     SDLERAEREKTGIGSLKIGFTSVFGYYLEVSKVNLSKVPPEWIRKQTLTNAERFVTPE
                     LKAHEDKVLSAEEKAKSLERELLAELRQKILAHREPLIRLAEGLAETDALASLAEAAE
                     RGHWTRPRLTNDPVLRLEGARHPVVERMLEATGGGAFVPNDLELDGTERQLLLVTGPN
                     MGGKSTYLRQSALIVLLAQMGSFVPAARATVGLVDRIFTRIGAGDNLAAGASTFLVEM
                     QEVANILHNATPRSLVILDEVGRGTSTYDGVAVAWAVAEHLAGRGAAGPRTLFATHYF
                     ELTELPDRFPRVANVHAAVREWARPDGKMDLVLLHEVRPGPAERSFGVHVAQMAGLPA
                     DCVTRAAAILAALEKGDARLPARPAGNGQMDLFANHPVLEALRVLDPERLTPLEALQK
                     LAELKRGL"
     gene            complement(3682..4254)
                     /locus_tag="PK362_00430"
     CDS             complement(3682..4254)
                     /locus_tag="PK362_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNC73340.1"
                     /translation="MIPGLKRRPRRWMLFAPALALLVPLAVPARFYWQVTRPLLHKDA
                     IDRYAGQYGIDPLFVLALVRVESGFAPSARSHRGAIGLMQLMPDTARDMAIRVGENPA
                     TLNLEEPDTNIHLGVHYLSLLKKEFGDDEVALLAAYNAGPTNTRAWRVGGRLTARDIP
                     FPETRVFVTRVRRTHRWLRRLRSIKRFFHV"
     gene            complement(4251..5573)
                     /gene="miaB"
                     /locus_tag="PK362_00435"
     CDS             complement(4251..5573)
                     /gene="miaB"
                     /locus_tag="PK362_00435"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNC73341.1"
                     /translation="MKFYVETFGCQMNVADSQEMGRRLAARGLAPTETAADADVILVN
                     TCTVRDHAEHKALSYLGRLTPWKSQDPSRCVILAGCAAERLQGTIRRRFPHVSVVVGA
                     KSIDRFDELIDAALPALAFDGDGETLDAWGWMKGAAETPSSVTAHVTLMRGCNFSCSY
                     CVVPQVRGREKYRPAASLLAEVREKAARGQPEILLLGQTVNSYRPAGPNPGPDGREIR
                     DFADLLRAVAAEPGVRRIRFMSPHPHHVDDRFIAAVAETPAVCPHVHLPAQSGSDRLL
                     PLMRRNYSRRDYVEKAARLRRAVPGLSLTTDLIVGFPGETDADFAETLSLVREVDFEG
                     AYTFKYSPRPGTASAALPDDVSTEVKEERLARLREWTDRLAEQKTAAQVGARTEALIE
                     ARRGKALEARTPHGRKIFLEGSDAPAGALVPCRVDRADGKTLYGSPCP"
     gene            5683..7038
                     /locus_tag="PK362_00440"
     CDS             5683..7038
                     /locus_tag="PK362_00440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNC73342.1"
                     /translation="MKATPKKVVICGGVRTPIGHLGKSLAGFLPEDLMEAAVRGVFQK
                     TSLYPHAVDGVLVGWVGQGSHAPNIARVTALKAGLPVKAHAYTVQANCVSSLETVASA
                     YRHIVMGEGDLYLAGGTESMSNFPYAIRGPRSHKKLRSLETVKAEWANLWGDAEIAIR
                     DTTEEGLTDPIEKINMAATAEICAQMYSISRAAQDKYAFDSYRKGLAGEKTGFYATHV
                     VPVERSGQIVLEKDEYPFLRESLVEKPKMLEKAPTIFDGPSYSIQDFYRDNGSYIQGK
                     TYEEGRSEGTVTLFNACARSDGAAVVIVCSEERAKDLGLEILAEIYSWGFWGTNPAYM
                     GISPVFATNIALERADLSFGELSHIELHEAFAATCLSIFRVGKEKFNQDWAAANESGA
                     LNPNGGTLALGHPLAATGVRLLLNLLYSMKSDPNARFGLSTACASGGLGGAMILKKYG
                     A"
BASE COUNT         1261 a         2253 c         2398 g         1259 t
ORIGIN      
        1 cggtcctccg agagaattcg cagttcaagc cccggttccg ccaaggccac gtcctccacc
       61 gtgcggagga gaaggccccg ctcggtggcg tccgatttca ggaatttcag tcgggcggga
      121 acggcgaaga acaaatcgtc cacggtgatc gtggtgcccg ggggaacgcc ggccgggcct
      181 tcccgcagga accggccccc ttccagggcg atggaccagc cttcgccctc ggcggagcgc
      241 gtggacaaac cgaagcgcga aacggcggcg atggaaggca aggcttcgcc gcgaaatccg
      301 taggtcgaca gggtttccag atcggtcaat tccgaaattt tggaggtggc gtggcgttcg
      361 agagcgaggc gcgcgtcgtc gggggtcatg ccccccccgt cgtcggtgac gcgcaggcgg
      421 cgccgaccgg cgtcccgcca ttcgatttca atgcggcggg cgccggcgtc caccgcgttt
      481 tcgaggagtt ccttgaggac ggaggcgggc cgttcgacga cctcgcccgc cgcgatgccc
      541 cggacgatgt cgtccggcag ctttcggact ttgttcttca atgcttcaac ttgacgggtt
      601 tgccgcagag ggtccaatag gcgcattgac ggaagcagac gtctttgagc ccgaaggaaa
      661 ttttgagttc gcagtcctgg aagtcttcgg ccattttcgt gatgcggtgg acggtgtcgt
      721 gggaaaggtg gtcgaatcgg accccaatgg ccgtcgtgtc gcctttgact tcggtccagg
      781 ccacgcggcc ctgaagttcg agattctcca acccgtggac gtccaaaagg attttcagtt
      841 tggcgtcgcc ggacacgtgg gcgaaaacga cgagggccat gccccccgcg gacaggttgg
      901 tcaggacggc cggaacttcg cggggcggct ggtccatgac gaacagatcc accggctcgg
      961 ccatgtcttt caaaacggga aagcgtttgt gtcgtcggtt gtcgggtttc aagggaccct
     1021 ccttcaaggg gcgcgtcgga ctccgcggtc gaatgataac aaagccgtcc ccgcgcgtcg
     1081 agtcccgggc gggttcacaa tccccttttc aattcggcca atttctgcag ggcttccagg
     1141 ggcgtcaggc gctccgggtc caggacgcga agcgcctcca ggacggggtg attggcaaac
     1201 aagtccattt gcccgttgcc ggcgggacgc gcgggtaggc gggcgtcgcc tttttcaagg
     1261 gcggccaaaa tcgccgcggc gcgggtgacg cagtccgccg gaaggcccgc catttgggcc
     1321 acgtgcacgc cgaaggatct ctcggccggt cccgggcgaa cttcgtgcag aagcacgagg
     1381 tccattttgc cgtcgggcct cgcccattcc cggacggcgg cgtggacgtt ggccacccgg
     1441 ggaaaccggt cgggcagttc ggtcaattcg aaatagtggg tggcgaaaag cgtccggggg
     1501 ccggccgcgc cccggccggc caggtgttcc gccacggccc aggccaccgc cacgccgtcg
     1561 taggtcgacg tcccccggcc cacctcgtcc aggatcacga ggcttcgggg ggtggcgttg
     1621 tgcaggatgt tggcgacctc ctgcatctcc accaagaaag tcgaggcccc cgccgccaaa
     1681 ttgtccccgg cgccgatgcg ggtgaaaatc cggtcgacca gtcccaccgt ggcccgggcg
     1741 gcgggcacga aggatcccat ttgcgccagg agaacgatca aggccgactg acgaagataa
     1801 gtggattttc cccccatgtt gggaccggtg accaacagca gttgccgttc ggtcccgtcc
     1861 aactccagat cgttgggcac aaaggccccg cccccggtgg cctccagcat tcgttcgacc
     1921 accgggtgcc gggccccttc caggcgcagc acggggtcgt tcgtcaaacg ggggcgggtc
     1981 cagtggccgc gttccgcggc ctcggccaga gacgccaggg cgtcggtttc ggccaatccc
     2041 tccgccaggc ggatcaaggg ctcccggtgg gcgagtattt tttggcgcaa ttcggccagg
     2101 agctcccgct ccagggactt ggccttttct tcggcgctca agaccttgtc ctcgtgggcc
     2161 ttgagctccg gcgtcacgaa ccgctccgcg ttcgtcagcg tttgcttgcg gatccattcc
     2221 ggcggaacct tggacaaatt gactttggac acctcgaggt aataaccgaa caccgaggtg
     2281 aacccgattt tgagagaacc gatgccggtt ttttcccgtt cggcccgctc caaatccgaa
     2341 atccacccgc gcccgtttcg cgccaattgg cgccattcgt ccaggggggc ggaatagccg
     2401 tcccgaatca cgcccccgtc ctggagcctc tggggcgggt cttcggccag ggctttctcg
     2461 aggagttccc cggggcccgg gggaacctgg aaactccggg tcagggaccc gagggggtgt
     2521 tggccggcga ggggatctcc gccggaccgc agggtgcgtt ccagatccgg cgcctgtcgc
     2581 agggtacgtc gaagggcgtt taagtcccgt cccgaggccg tgcccgcggt gagtcgggag
     2641 aggacccgtt ccacgtcggc cgtttcacgc aacaaatcaa cgacggcccg gcgcagccgt
     2701 ccgtggtgga ggaaataatc cacccggtcc tgccgggcgg cgatttcctc caaatccatc
     2761 agagggttca ccatccatcc cttgaggcgg cggccgccca tggcggtggc cgtgtgatcc
     2821 agtaaatgcc aaagggacgt cggtcgctgc gggtcgccgc ccgaatccga cggaaataag
     2881 tccagccggc gcaaggccag ggcgtccagc gccatgaagc gcgtcggggc gtgggcccgg
     2941 ggcgggcgca gagcgccgag ggccggggcc tggttgcgtt cgatgtaatt gagcagggcg
     3001 gccacggcgg gtagggccgg atggtcggcg gaaaggccgt agccttcaaa ggacttcacg
     3061 ccgaaaaggg cgggcgcccg gcggagggcg gaatcgggca aaaaagtcga cggttccacg
     3121 cgcgtcacgg ctcgatcgcc gtccgggtcg gggcctccgg cgggccacag cgtttcccgg
     3181 ggatcccaac gggacaattc atcggccacg gcgccgccgt cgggcagcga cgcgaaggac
     3241 aattcgccgg tggacatgtc ggcccaggcc aggccccagg gcgacccgcc gcgccccggc
     3301 cacgcggccg ccaaaaaatt gttttgcttg gccggtagca actcctcttc cagcacggtg
     3361 cccggcgtca ccacgcgcac gacgtcccgt cgcaccatgc ctttcgtggt ggccgggtct
     3421 tccatttgtt ccgcgatggc gacgcgccag ccccgcttca gcaatttgcc caggtacccc
     3481 ggcagggcgt ggtgcggaag gccgcacatg gggatgccct ggcgttgggt gaggaccaat
     3541 tcaaggatcg gggcggcgcg gcgggcgtcc tcatcgaaga gttcgtagaa atcccccaaa
     3601 cgaaaaaaaa ggatggcgtc gggattttgg gccttaagcg cctggtattg ccgcatgatg
     3661 ggcgtggcgg ccgcggtggt ttcagacatg gaaaaacctc ttgatcgacc ggagtcttcg
     3721 cagccaccga tgggttcgac ggacgcgggt cacgaacacg cgggtttccg gaaaggggat
     3781 gtcccgggcg gtgagccgcc cgccgacgcg ccaggcccgg gtgttggtgg gaccggcgtt
     3841 gtaggcggcc aggagagcca cctcgtcgtc gccgaattcc ttttttaaca gggacaaata
     3901 gtgcacgccc aggtggatgt tggtgtccgg ttcctccagg ttcagggtcg cggggttttc
     3961 ccccacgcga atcgccatgt cgcgggcggt gtccggcatg agctgcatga gaccgatggc
     4021 gccccggtgg gatcgggccg agggggcaaa acccgattcg acccgcacca aggccaacac
     4081 gaagagagga tcgatgccgt attggccggc gtatcggtcg atggcgtcct tgtggaggag
     4141 cggccgcgtc acttgccaat aaaatcgcgc gggaacggcc agcggcacaa gtagggccag
     4201 ggccggcgca aaaagcatcc agcgccgggg acgccgtttg agccccggaa tcatgggcac
     4261 ggggatccgt aaagcgtttt cccgtcggcc cggtcgacgc ggcagggcac cagggcgccc
     4321 gccggggcgt cggacccttc caggaaaatt ttccgaccgt ggggggtccg ggcctcgagg
     4381 gcctttcccc ggcgggcttc gatcaaggct tcggttcggg cgccgacctg ggcggcggtt
     4441 ttttgttccg ccagccggtc ggtccattcc cgcaaacggg ccaaccgttc ttctttgacc
     4501 tcggtggaaa cgtcgtcggg cagggcggcc gacgccgtgc cgggccgggg cgagtattta
     4561 aacgtgtagg cgccttcaaa atccacttcc cgcaccaggg acaatgtttc cgcgaaatcg
     4621 gcatcggtct cacccgggaa tcccacaatg agatcggtgg tgagggacag gcccggaacc
     4681 gcccggcgca accgggccgc tttctcgacg taatcccgcc gggagtaatt gcgacgcatg
     4741 aggggcaaaa gccggtccga tcccgattgg gcgggaaggt ggacgtgggg gcagacggcg
     4801 ggcgtttccg cgaccgcggc gatgaaccga tcgtccacgt ggtgggggtg ggggctcatg
     4861 aaacgaatgc gccgcacgcc gggttccgcc gccaccgccc gaaggaggtc ggcaaaatcc
     4921 cgaatctccc ggccgtcggg gcccgggttc ggaccggccg gccggtagga gttgacggtt
     4981 tgccccagca aaaggatttc cggttggccc cgggcggctt tttcccggac ttccgccaac
     5041 aaactggccg ccgggcggta tttttcccgc ccccgcacct gggggacgac gcagtagctg
     5101 caggaaaaat tgcacccccg catgagggtt acgtgcgccg tgaccgagga tggcgtttcg
     5161 gcggccccct tcatccagcc ccaggcgtcc agggtttctc cgtcgccgtc aaaggccaag
     5221 gcggggaggg ccgcgtcgat caattcatcg aaccggtcga tggatttggc ccccacgacc
     5281 acggagacgt gggggaagcg ccggcggatc gtcccctgca accgttccgc cgcgcaaccc
     5341 gccaggatga cgcaccggct ggggtcctgg cttttccagg gggtgagacg gcccagatag
     5401 gagagggcct tgtgttcggc gtggtcccgc accgtgcagg tgttgaccag gatcacgtcg
     5461 gcgtcggccg ccgtttccgt gggggcgagc ccccgggcgg ccaggcgacg gcccatttcc
     5521 tggctgtcgg ccacgttcat ctggcacccg aaggtttcga cgtaaaattt cataatccgc
     5581 ttattttacc atgcccgggg cccgtcggcg cggcgattga caaagccgcg cccgggggct
     5641 agccttaaag cgcaaccggt ccacacgctc gggaggacac ccatgaaggc cacgcccaaa
     5701 aaagttgtga tttgcggagg ggtgcgaacc ccgatcggac atctgggcaa aagcctggcg
     5761 gggttccttc ccgaagacct gatggaagcc gccgtgcgcg gcgtctttca aaaaacgtcc
     5821 ctctatcccc acgccgtgga cggcgtcttg gtcggttggg tgggtcaagg gtcccacgcc
     5881 cccaacatcg cccgcgtcac ggccctcaaa gccggactgc cggtcaaagc ccacgcctac
     5941 acggtccaag ccaattgcgt ttcaagtttg gaaaccgtgg ccagcgccta tcggcacatc
     6001 gtcatgggcg agggcgatct gtatctggcg ggtggcacgg aatccatgtc caatttcccc
     6061 tacgccatcc gcggtccccg ctcccataaa aaactgcgtt ccctggagac ggtgaaggcc
     6121 gaatgggcca acctctgggg cgacgccgag atcgccatcc gggacacgac cgaagaaggc
     6181 ctgacggacc ccatcgagaa aatcaacatg gccgccacgg cggaaatctg cgcccaaatg
     6241 tattcgatct cccgggcggc ccaggacaaa tacgcctttg acagttatcg caaaggcctg
     6301 gccggagaga aaaccggatt ttacgccacc cacgtcgtac ccgtggagcg gagcggccag
     6361 atcgtgttgg agaaggacga ataccccttt ttgcgggaga gtctcgtgga aaaaccaaaa
     6421 atgctggaga aagcgccgac gatttttgac ggaccctcct attccatcca ggatttttac
     6481 cgggacaacg ggtcgtacat ccaggggaag acctacgagg aaggtcggag cgagggcacg
     6541 gtgacgctct ttaacgcctg cgcccgctcg gacggcgccg ccgtcgtgat cgtttgctcg
     6601 gaggaacgcg ccaaggattt ggggttggaa attttggcgg aaatctattc ctggggtttc
     6661 tggggaacga acccggccta catggggatt tcgccggtgt tcgccaccaa catcgccctg
     6721 gagcgggcgg atttgagttt cggggagctt tcccacatcg agctgcacga ggccttcgcg
     6781 gccacctgcc tctccatttt ccgggtgggc aaggaaaaat tcaaccagga ctgggcggcg
     6841 gccaacgagt cgggcgcctt gaaccccaac ggcggcaccc tggccctggg tcatcccctg
     6901 gccgcaaccg gcgtgcggct cctgctcaat ctcctctatt ccatgaaatc cgatcccaac
     6961 gcccgcttcg gcctttccac cgcctgtgcc tccggcggac tcgggggggc catgatcctt
     7021 aaaaaatacg gcgcctgaac ccctccgtcc attacgtcga cggggatcga tcgttttggt
     7081 cgatccccgt ttttttttgg ttaaggtcgt ggatgaaatt gccggtgggc ccgtttgagg
     7141 gcttcccggt ccacgtgggt gtaaatttgg g
//
LOCUS       DAOQLS010000013         6791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_4692_length_6791_cov_4.746734, whole genome shotgun sequence.
ACCESSION   DAOQLS010000013 DAOQLS010000000
VERSION     DAOQLS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4692_length_6791_cov_4.746734"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="gcvT"
                     /locus_tag="PK362_00445"
     CDS             <1..835
                     /gene="gcvT"
                     /locus_tag="PK362_00445"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNC73343.1"
                     /translation="YALLCKPDGGVIDDLYIYCLETDRFLVIVNASRAAVDIKWMKEN
                     LSGDAMLIDQPQAAALALQGPSAAKIMGHFSADALALPKNGVAELTIEGNELVVARTG
                     YTGEDGFEIFSPAGHLLQFYPSLLKRGSDYGLIPCGLGARDTLRLEMGYRLYGNDLDE
                     HHSALEAGLGWAVKFGKGDFIGRDSLFNEKTKGSHRKFVGLRLTERGVPRHGCPIVLD
                     GKAVGEVTSGTFSPSLNIGIAVGYVEAERFPAGAVRAAVRVHERDIPADVVVPPFYKK
                     S"
     gene            844..1221
                     /gene="gcvH"
                     /locus_tag="PK362_00450"
     CDS             844..1221
                     /gene="gcvH"
                     /locus_tag="PK362_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNC73344.1"
                     /translation="MDPLQLRFTPSHEWIAPDGTVGISDHAQHEITDVVFVELPKAGR
                     AVKAKESCAIVESVKAAFDIYAPVAGSVSAVNDAVVKNPALVNQAPYEGGWLYKIAMS
                     NPAERDGLMTHAQYQEFIKTAAH"
     gene            1238..2191
                     /locus_tag="PK362_00455"
     CDS             1238..2191
                     /locus_tag="PK362_00455"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HNC73345.1"
                     /translation="MAKTVLVTGAFSYIGAAVAEELMRRGHRVRTLTNRAVPTGLAET
                     VPTQPLRFEKEFLVGAMKGVDVFINTYWIRFPHAGQTFQTAIVNSERLFEAARDAGVG
                     RIVHVSVSNPDRGSDLGYYRGKADVEWKLRQNGVPHAIVRPTLVVGPGDVLTNNIGWL
                     LRRFPVFALPQGGRCRLQPVTLRETARIICDNIDGPDGSVVDAAGPETFSFAYFVRRL
                     AALLNVRRFFLPVPNGLALACLAPLGKCLRDTILTAEELLGLEQELLVSRRPPLSGES
                     VWPWLEAHRTDVGRVYVNDKNRHFGSGKTKPLHDSFRADLR"
     gene            2233..3561
                     /gene="gcvPA"
                     /locus_tag="PK362_00460"
     CDS             2233..3561
                     /gene="gcvPA"
                     /locus_tag="PK362_00460"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNC73346.1"
                     /translation="MFVPHTEEQKKEMLRSIGVSKFDDLLAGLPPSLLRPNLGLPSSL
                     SEMELLRHMEGLAQKDHLAQSYLGAGAYEHFIPTSVWALALRGEFATAYTPYQAEASQ
                     GTLQSIYEFQSLIGELFAMDVANASMYDGASAAAEACLLAAKHTERPVILIPETVHPQ
                     TRQVIETYLAHSGARIVVLPCPDGVLSLETLKKNLGGDTAALLIQNPNFFGCLETNIH
                     EVAAAVHAAGGLVIASSYPVALGMISPPGEYGADIAVAEGQPLGLPLGYGGPYVGLFA
                     CKTELIRKMPSRIVGQTVDRDGKRAFVLTLQAREQHIRREKATSNICTNQALCALAST
                     IHMSLLGKEGLRELADLNFHKAHYAAGELQKRGFAPVFQQPFFNEFTVRCPVSPEKIA
                     DRLAEKRVLAGLPLGPYFKDRADQLVVCVTEIKTKADIDEFAGLLEAACR"
     gene            3552..5030
                     /gene="gcvPB"
                     /locus_tag="PK362_00465"
     CDS             3552..5030
                     /gene="gcvPB"
                     /locus_tag="PK362_00465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNC73347.1"
                     /translation="MPLTQLPAVPGIVETLIFEKSVPGRKGVPLPGLDVPRTAPSALP
                     DKLRRKKPAAWPELSEPQVVRHFTRLSQLNFSIDTNFYPLGSCTMKHNPKVNDRVAML
                     PDFSALHPLRPHTLSQGILEILFELERWLCRICGMDAFTLQPSAGAQGELTGILVARA
                     YHESRGQNRPLVLIPDSAHGTNPASVAVAGLTAVTIKSAADGQVDLGDLKAKLTDQVG
                     LVMMTIPSTLGMFEPRIQEIAALVHKAGALLYMDGANMNALVGLLRPGDIGVDILHLN
                     LHKTFSTPHGGGGPGAGPVGVKKELEPFLPVPRVAKDAGVFRRKFDRAKTIGRVHGFQ
                     GNIGVLIRAYCYMRLQGAEGLLEISENAILAANYVRARLTPLFPKMPKNPCLHEVVVS
                     GEGAYGPGVKTLDVAKRLLDYGFYAPTIYFPLIVPEAMMIEPTENESKETLDAFCATM
                     EKIIEESKAQPDLVKNAPHTTPVRRLDEVKAAREPNLRYAPR"
     gene            complement(5027..5356)
                     /locus_tag="PK362_00470"
     CDS             complement(5027..5356)
                     /locus_tag="PK362_00470"
                     /inference="COORDINATES: protein motif:HMM:NF026918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 53 family protein"
                     /protein_id="HNC73348.1"
                     /translation="MSEATPLERLQRWFAAQCNGTWEHEWGVKIETLDNPGWLVRIDL
                     AGTPWAAKEFPRRKEGINDAGHPVAERWIHCFTQDQLFLGACDAHQLGRVIELFLAWV
                     DDAPPTA"
     gene            complement(5353..5718)
                     /locus_tag="PK362_00475"
     CDS             complement(5353..5718)
                     /locus_tag="PK362_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC73349.1"
                     /translation="MIRRLDAPAVVAAAGNKPKKIEEFIGRVNSGHSSVSIARMTSPA
                     GWTEPGQTPEFDEFSLVLRGTLRVESRDSVLEVGPGQSVVAPRGEWVRYSTPGPEGAE
                     YIAVCLPAFSPHSVHREDA"
     gene            complement(5800..6174)
                     /locus_tag="PK362_00480"
     CDS             complement(5800..6174)
                     /locus_tag="PK362_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC73350.1"
                     /translation="MQTWIFVLIGLAAGLVSGFFGLGGGTIIVPALTYFAGFSQHRAV
                     GTSLAVLLLPAGLGAVMEFHRQGNVDLRAAGLMAVALFASAWISGRWANRVAPAPLKL
                     AFGIFLVLVGARIVFSTAGKFR"
     gene            complement(6175..6576)
                     /locus_tag="PK362_00485"
     CDS             complement(6175..6576)
                     /locus_tag="PK362_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC73351.1"
                     /translation="MRTPRLRARRPVTRRRTAALCWLLALLGAVPGVGRADDDARPST
                     APVYIDVRTPEEFAAGHIQGARLLPYQTIEKTIGLSVPDKETPLVLYCAVGGRSGIAL
                     KKLKKMGYRNAVNGGGYEDIKRRLSIQGKAP"
BASE COUNT         1259 a         2176 c         2120 g         1236 t
ORIGIN      
        1 gtacgccttg ctttgcaagc ccgacggcgg cgtcatcgac gacctttata tttattgcct
       61 ggagacggac cgcttcctgg tgatcgtgaa cgcctcccgg gccgcggtgg acatcaaatg
      121 gatgaaggaa aatctgtccg gggacgcgat gctgatcgat cagccccagg ccgccgccct
      181 ggcgcttcag gggccctccg cggccaaaat catgggccat ttttccgccg acgccctggc
      241 ccttcccaaa aacggcgtgg ccgagctgac catcgagggc aacgaactgg tggtggcccg
      301 gacggggtac acgggcgagg acggctttga gattttttct ccggcggggc acctgcttca
      361 attttacccg tcgctcctca agcgcggctc cgactacggg ttgattccct gcggcctggg
      421 agcccgggac actctgcgtt tggagatggg ctaccgactt tacgggaacg atctggacga
      481 gcaccactcc gccctggaag ccggtctcgg ctgggcggtc aaattcggca aaggcgattt
      541 catcggacgg gattccctgt tcaacgagaa aaccaaggga tcccaccgga aattcgtcgg
      601 attgcgcctg accgaacggg gcgtgccccg ccacggttgc cccatcgtgt tggacgggaa
      661 ggccgtgggc gaggtgacga gcggcacctt ttccccgtcc ctgaacatcg gcatcgcggt
      721 gggctacgtc gaagccgagc ggttccccgc cggcgccgtc cgggccgccg tccgggttca
      781 cgagcgggac atccccgcgg acgtcgtcgt tccgcctttt tataaaaaaa gttaggagaa
      841 cgtatggatc cccttcaact gcgtttcacc ccgagccacg aatggatcgc ccccgacggc
      901 accgtgggca tttccgacca cgcccagcac gaaattaccg acgtggtgtt cgtggagctg
      961 cccaaggcgg gccgcgccgt caaggccaag gaatcctgcg ccatcgtgga atcggtcaaa
     1021 gccgcttttg acatttacgc cccggtggcc ggttccgtga gcgcggtgaa cgacgccgtg
     1081 gtcaaaaacc ccgcgttggt caaccaagcg ccctacgaag gcggctggct gtataaaatc
     1141 gccatgtcga acccggccga gcgcgacggc ctcatgaccc acgcccagta tcaggaattc
     1201 atcaaaaccg cggcgcactg acccccgggg gtcccccatg gccaaaaccg ttctcgttac
     1261 gggggccttt agttatatcg gagcggcggt ggccgaggaa ttgatgcgcc ggggccatcg
     1321 cgtccggact ctgaccaacc gggccgttcc gaccggtctc gcggaaaccg tcccgaccca
     1381 acccctccgg tttgaaaaag aatttttggt cggcgccatg aagggcgtgg acgtttttat
     1441 caatacgtat tggatccgtt ttccgcacgc gggacagacc ttccaaacgg cgattgtgaa
     1501 ttccgaacgg ctttttgagg cggcccggga cgcgggcgtc ggtcgaatcg ttcacgtgag
     1561 cgtcagcaac ccggaccgtg gatcggacct ggggtattac cggggaaagg ccgacgtcga
     1621 atggaagctg cggcaaaacg gcgttccgca cgcgattgtc cggccgacgc tggtggtggg
     1681 gcccggcgac gtgctcacca acaacatcgg gtggctgctt cgacggttcc ccgtttttgc
     1741 cctgccgcaa ggcggtcgat gccgcttaca gccggtgacg ctgcgggaaa cggcccgtat
     1801 tatctgcgat aatatcgacg gaccggacgg gtcggtcgtg gacgccgcgg ggcccgaaac
     1861 gttttcgttc gcgtatttcg ttcggcggtt ggccgcgctg ctcaacgttc gacgcttttt
     1921 ccttccggtg cccaacggcc tggcgttggc ttgtctggcg cctttgggaa aatgcctgcg
     1981 ggacacaatc ctgaccgcgg aggaattgtt ggggttggag caggaattgt tggtgtcccg
     2041 gcggccgcct ttgagcgggg aatctgtttg gccgtggctc gaagcccatc ggaccgacgt
     2101 gggtcgggta tacgtcaacg acaagaaccg tcatttcgga tcggggaaaa caaagcccct
     2161 tcacgattcg ttccgggcgg accttcgctg attttcgctg atttgaataa cgtcgttcaa
     2221 ggatagccct ttatgttcgt cccccacacc gaagaacaaa agaaggaaat gctccggagc
     2281 atcggggtgt caaaatttga cgacctcctg gcggggttgc cgccgtccct tttgcggccg
     2341 aacctggggc tgccgtcctc cctgtccgag atggaactcc ttcgccacat ggaaggtttg
     2401 gcccaaaagg accacctggc ccagtcctac ctcggggcgg gcgcctacga acatttcatt
     2461 ccgacgtccg tctgggccct ggcccttcgg ggagaattcg ccacggccta cacgccgtac
     2521 caggccgagg ccagccaagg cacgcttcaa tcgatctacg aattccagtc gctgatcggc
     2581 gaactcttcg ccatggacgt ggccaacgct tcgatgtacg acggggccag cgccgccgcc
     2641 gaggcttgtc ttttggccgc caaacacacg gaacggcccg tcattctgat ccccgaaaca
     2701 gtccaccccc aaacgcggca ggtgattgaa acctacctgg cccacagcgg tgcgcggatc
     2761 gtcgttctgc cctgccccga tggcgtgttg tccctggaga cgttgaagaa aaacctgggc
     2821 ggggacaccg cggccctgtt gattcaaaac cccaactttt tcggctgtct cgaaacgaac
     2881 atccacgagg tcgccgccgc ggtccacgcc gcggggggtc tcgtcatcgc ctccagctac
     2941 cccgtggcgc tcggaatgat ttcgcctccg ggcgaatacg gcgccgacat cgccgtggcc
     3001 gagggacagc ccctgggcct gcccttgggc tacgggggcc cctacgtggg gctcttcgcc
     3061 tgcaaaaccg aattgatccg caaaatgccc agccgcatcg tgggccagac ggtggatcgg
     3121 gacggcaagc gggcttttgt cctgaccctc caggcccggg agcaacacat ccgccgggaa
     3181 aaggccacct cgaacatttg cacgaaccag gccctctgcg ccttggcgag cacgattcat
     3241 atgtcgcttt tgggcaagga gggtttgcgg gagctggcgg atttgaattt ccataaagcc
     3301 cactacgccg ccggggaact tcaaaagcgc ggcttcgcgc cggtgttcca acagcccttc
     3361 ttcaacgagt tcacggtccg ctgtcccgtg tcgccggaaa aaatcgccga ccgactggcg
     3421 gagaagcgcg tgctggccgg gcttcccctg ggcccctatt tcaaggaccg ggccgaccaa
     3481 ctggtcgttt gcgtcacgga aatcaaaacc aaggcggaca tcgacgaatt cgccgggctt
     3541 ctggaggccg catgccgttg acccaactcc ccgccgttcc cggaatcgtg gaaaccctga
     3601 tttttgaaaa gagcgttccc ggtcgaaagg gcgttcctct gccgggcctg gacgtgcccc
     3661 gaaccgcccc ctcggccctg cccgataaac ttcgacggaa aaagcccgcg gcctggccgg
     3721 aactctcgga gccccaggtg gtgcgccatt tcacgcggct ctcccagttg aatttcagca
     3781 tcgacacgaa tttttatccc ctggggtcct gcaccatgaa gcacaacccc aaggtcaacg
     3841 accgggtggc catgctgccg gacttctccg ccctgcaccc cctgcggccc cacaccctga
     3901 gccagggcat cctggaaatc ctcttcgagc tcgagcggtg gctgtgccgg atttgcggca
     3961 tggacgcttt cacccttcag ccctccgccg gcgcccaggg cgagttgacc ggcattctgg
     4021 tggcccgggc ctaccacgaa tcccgggggc agaaccgtcc cttggtgctc attcccgatt
     4081 ccgcccacgg caccaacccc gccagcgtgg cggtggccgg ccttacggcg gtgacgatca
     4141 agtccgccgc cgacggccag gtggacctgg gggatttgaa ggcgaaactc acggaccaag
     4201 tgggcctggt gatgatgacg atccccagca ccctgggcat gttcgagccg cggatccagg
     4261 aaatcgccgc gctggtccac aaggccggcg ccctgctcta catggacggg gccaacatga
     4321 acgccctggt ggggttgttg cggccggggg acatcggggt ggacattctg cacctgaacc
     4381 tgcacaaaac cttttcgacg ccccacggcg gcggcggtcc cggcgcggga cccgtggggg
     4441 tcaaaaaaga attggagcct ttcctgcccg tgccccgggt ggccaaggac gccggcgttt
     4501 ttcgacggaa attcgaccgg gccaagacca tcggccgggt gcacgggttc caagggaaca
     4561 ttggggtctt gattcgggcc tattgctaca tgcgcctgca gggcgcggag gggttgctgg
     4621 aaatcagcga aaacgcgatt ctggcggcca actacgtgcg ggcccggctg acgccgttgt
     4681 ttccgaaaat gccgaagaat ccctgtctgc acgaggtggt ggtgtccggc gaaggggcct
     4741 acggtcccgg ggtgaaaacc ttggacgtgg ccaagcggct gctggactac ggtttttacg
     4801 cgccgaccat ctatttcccg ctcatcgttc cggaagccat gatgatcgag cccaccgaaa
     4861 acgagtccaa ggaaactctg gacgcttttt gcgcgaccat ggaaaaaatc atcgaagaat
     4921 ccaaagccca gccggacctc gtcaaaaacg cgccccacac gacccccgtc cgccgcttgg
     4981 acgaggtcaa agccgctcgg gagcccaacc tccgctacgc gccccgctag gcggtcggcg
     5041 gggcgtcgtc cacccaggcc aggaacagct ctatcactcg gcccagttga tgggcgtcgc
     5101 aggccccgag gaaaagttgg tcctgggtga agcagtggat ccaccgctcc gccacggggt
     5161 gccccgcgtc gttgatgcct tccttgcgcc ggggaaattc cttcgccgcc cagggcgttc
     5221 ccgccaaatc gatccgcacc aaccaccccg gattgtccag ggtctcgatc ttcacgcccc
     5281 actcgtgttc ccaggttccg ttgcattggg ccgcgaacca gcgctgcagt cgttcgaggg
     5341 gggtggcctc gctcacgcgt cctcccggtg cacgctgtgg ggggaaaagg cggggaggca
     5401 cacggcgatg tattcggcgc cctcgggtcc cggggtgctg tagcggaccc attcgcctcg
     5461 gggggccacc accgactgcc ccggtcccac ctccaaaacg gagtcccggc tttccacccg
     5521 aagggtcccg cgcaagacca atgaaaattc gtcaaattcg ggggtttgcc cgggttccgt
     5581 ccatccggcc gggctcgtca ttcgggcgat gctaacgctg gaatggcccg aattgacgcg
     5641 gccgataaac tcctcgattt ttttgggctt gttgccggcg gcggcaacga cggcgggcgc
     5701 gtccaaacgg cggatcatcg aagggtcctt tccataccgg cccagggaat ccggatcgat
     5761 gggcgaacgg cggtcgaggt catgggatcc gggggccctt taacgaaatt tccccgcggt
     5821 ggaaaagacg atccgcgccc ccaccaatac gaggaagatc ccgaaagcca acttgagcgg
     5881 cgcgggggcc acccggttgg cccaacggcc gctgatccag gccgaggcga aaagggccac
     5941 ggccatgagg cccgccgccc gaaggtccac gttgccctgg cgatgaaact ccatcaccgc
     6001 gcccaatccc gccggcagga gaagaaccgc gaggctcgtt cccacggccc ggtgttggga
     6061 gaaccccgcg aaatacgtca gggccggcac gatgatcgtt cccccgccca atccgaaaaa
     6121 acccgaaacc aatcccgcgg ccaaaccgat caacacgaag atccacgttt gcatttaagg
     6181 cgcctttcct tggatcgaca atcgtctttt gatgtcctcg tagccgccgc cgttcacggc
     6241 gtttcggtac cccatttttt ttagcttttt aagggcgatg ccggaccggc cgccgacggc
     6301 gcaatagagg accagggggg tctccttgtc cggcacggag aggccgatgg ttttttcgat
     6361 ggtttggtag ggcaagagcc gggcgccctg gatgtgaccg gccgcgaatt cctccggcgt
     6421 gcggacgtcg atatacaccg gggcggtgga aggccgggcg tcgtcgtcgg ccctcccgac
     6481 accggggacg gcgccaagca gagcgaggag ccaacaaaga gccgcggttc gccgtcgcgt
     6541 cacggggcgg cgggcgcgga gccggggggt tcgcatggga ccattatagc gcgggtttga
     6601 tatattcaac gatgtcccat gaaaaacgat ttgtgcgaaa aagaaattcg gggccccgct
     6661 ccgtggcgag tttccctgga aggcgccggc gccggggaag agaacatgcg ccgggacgcc
     6721 gaagccctgg acgtcctgcg cggggcgagg gagattccgc gacttcgcgt ttaccgctgg
     6781 gcggagccca c
//
LOCUS       DAOQLS010000014         6780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_4711_length_6780_cov_3.819628, whole genome shotgun sequence.
ACCESSION   DAOQLS010000014 DAOQLS010000000
VERSION     DAOQLS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4711_length_6780_cov_3.819628"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PK362_00490"
     CDS             complement(<1..447)
                     /locus_tag="PK362_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNC73352.1"
                     /translation="MTDLAPMSDAPSDALRAPVSEIFSSLQGEGLHLGERHVFIRFAG
                     CPWRCRYCDTPDSLTDEGHSRSTVQEVLERVGELVRDRPHAGISLTGGEPLLQTAFLE
                     SLLPGLKTLGLKIHLETSATHPHLFQRVADDVDVVAADIKLPSAIGR"
     gene            complement(444..1196)
                     /gene="queC"
                     /locus_tag="PK362_00495"
     CDS             complement(444..1196)
                     /gene="queC"
                     /locus_tag="PK362_00495"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009784435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNC73353.1"
                     /translation="MKGPATLRPPLHAPPRAVVLLSGGLDSSTALYWALRIKRWNCRA
                     IAFDYGQRHRRELRSARSLARAAGVPLDVVSFRLPWGGSRLLDRGPLPDHPLGAIGRG
                     PIPSTYVPARNTVFLSFALSCADALEIPNVVIGANALDYSGYPDCRPDFLRAMEKVGR
                     RGTRLGAELKKPPRLWAPLLRLDKAAIVRLAFRLGVPLARTWSCYAGKSRPCGRCDSC
                     RLRIEGFRRAGRADPADPQPKVVSSTGSRHRP"
     gene            complement(1222..2040)
                     /gene="murI"
                     /locus_tag="PK362_00500"
     CDS             complement(1222..2040)
                     /gene="murI"
                     /locus_tag="PK362_00500"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNC73354.1"
                     /translation="MGARPRPIAVFDSGVGGLTVLRALVSLLPREHFIYVGDTARVPY
                     GSKSPESVKRFSIEVARFFRRKNVKMMVTACNTVSALALPELRAFMPVPVLGVIEPGA
                     RAALAATRTGRVGIIGTEATVQSRAYEHAVQRLDGTIRVFSRACPLFVPLVEEGWLDH
                     PVTRRVASLYLAPLLRKKIDTLVLGCTHYPLIKGVLRRVAKTVELIDSAEETAKAVRS
                     RLDQDGLLVSRGPRGRVDYFSSDDPVKFSRLGHRFLGWNLPNVARIRLEGVDGV"
     gene            complement(2040..3620)
                     /locus_tag="PK362_00505"
     CDS             complement(2040..3620)
                     /locus_tag="PK362_00505"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNC73355.1"
                     /translation="MRTSKTALAVLVGVALLGAARRPPWPADSMTVVVDGETRGPASL
                     FRIDGIACLPLEHLQKIFGGRVTWKRVSRKFDYRLENRTAEFVLDSSTAVVGGQSVAL
                     ETSVRWWGDSAFLPVSLLTTPAYQSFTKAKIQWIESPPSLTVDPIPSISSARVFNYPQ
                     ETRVSVELGPDVDYRLLGQRDNTLYLRLFDGRSAQSEKLTFDEGTVASVEMTPHARTT
                     DLTVRLATGAGTPDIYTTASPRTLTIAVPKGAVWSPGRRSPPPDEEVEPVLRAPPADA
                     PRSSLDAPYLALSPLRTIVIDAGHGGKDVGAVGPGGTYEKDVNLRTARALAQLLRAEG
                     RFNVLLTRSDDRFLTLEERSSVANKNKADLFISLHCNAGLTRASNGFEIYYLSEKATD
                     DEAAAVARRENAVVDLEGVAGPAKEKVEELLWSLARNEHMNDSSEIAALIAHQAAKRL
                     PLANRGVKQAGFYVLRGTAMPAILIETGFITHPKEEGLLRSTRYHQKLIETVYAGLLD
                     YEKRKIQTRLAQSSAGGN"
     gene            complement(4106..4771)
                     /locus_tag="PK362_00510"
     CDS             complement(4106..4771)
                     /locus_tag="PK362_00510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC73356.1"
                     /translation="MIPLKDNIKGRSFPFVNILLILLNGAIFYLEIRQPSPKALMAFL
                     GQWSLVPGNLIRNPASEWIKILSSMFLHGGWMHFLGNMLYLWIFGDNVEDRMGHRRYL
                     IFYLLVGSCAALSQVCLNPHSTLPLIGASGAIAGVMGAYFILFPKAKITALVPIWIFI
                     RFIEIPAILFLGFWFLLQALQSWGSILNASAGGSEMGGVAWWAHAGGFLAGFLFVIPF
                     KKR"
     gene            complement(4768..6402)
                     /locus_tag="PK362_00515"
     CDS             complement(4768..6402)
                     /locus_tag="PK362_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC73357.1"
                     /translation="MLRRGPTLFFLLLSLLVAGGCWVWRRSTPWARAQRALDRGEAAD
                     ATLIAGGALAGRTWPRNREQLLRELLVESQLKLGALENAEKECRILHEKFPQDPSGAL
                     GLGILNLAFDRLSFAADYLEEARRLAPGDFRTILILANVRLAQNDYPRATEILEEGLK
                     LFPARSELLELYGDLAFDQGYFQKALARYGTVLRSVPEDRDTLFKITRAHLAAGDNES
                     AEASIRRLRRAPEDIEADLLLAGVRAQQGRRAEAEDLCARAYQGDHNRFQAGFSLAYW
                     LGIRGEEERSEAILSEINRRLPPLRSNADWSEPQDWDSVARNFQGRLYARGLQRDYEM
                     ARAQLDLHCHRAVDAGAHARRALDLDSNDAGVLLLLADIARRRGDNKDRLRWIDRTVE
                     LYREHPGALLSRGWAYLDLGRVSDAVVDAKLVSDAYPRLASAQALLSRAFLLIGRASE
                     AVDVAERAVGLNGGDVDAQLALGLSRSAEGKNNEAEKCFLKALEIDPFSAEARADYAW
                     FLRGQKRLDEAKKQWMEASHLEPSVFPALGLSKIKR"
BASE COUNT         1421 a         1923 c         2230 g         1206 t
ORIGIN      
        1 ccggccgatg gccgacggca atttgatgtc cgccgccacc acgtcgacgt cgtcggcgac
       61 gcgttgaaac aaatgggggt gggtggcgct ggtctccagg tggatcttca aaccgagggt
      121 tttaaggccg ggcaggaggg attccaaaaa ggccgtttga agcaaaggtt ctccgccggt
      181 gaggctgatc ccggcgtggg gacggtcccg gaccaattcc cccacccgtt ccaacacctc
      241 ttgaacggtg gatcgcgaat ggccttcgtc ggtcaaactg tcgggggtat cgcagtagcg
      301 gcagcgccag gggcaaccgg caaaacggat aaagacgtgg cgttcgccga gatggaggcc
      361 ttcgccctgg agggaggaaa aaatttccga gaccggcgcg cgaagggcgt cggagggggc
      421 gtcggacatc ggggcgaggt cggtcatggt cggtggcggg agcccgtgga ggataccact
      481 ttcggttgcg gatcggcggg atcggcgcgg cccgcgcgac gaaacccttc gatccggagg
      541 cgacaggaat cgcaccgacc gcagggccgg gattttccgg cgtagcagga ccaggtgcgg
      601 gcgaggggga cgcccagacg gaaagcgagg cgcacgatgg ccgccttgtc caggcggagg
      661 agcggcgccc aaagacgcgg cggttttttc agctccgcgc ccaaccgggt gccgcggcga
      721 cccaccttct ccatggcgcg aaggaagtcg ggccggcagt cggggtagcc ggaataatcg
      781 agggcgttgg cgccgatgac cacgttcgga atctccaggg cgtccgcgca ggagagcgcg
      841 aaggacagaa acaccgtgtt acgggcggga acgtaggtgg agggaatggg tccccggccg
      901 atggcgccga ggggatggtc gggcaacggg ccgcgatcca ggaggcggct tcccccccag
      961 ggcaggcgaa aggagacgac gtccaggggc acgccggcgg cgcgggcgag ggaccgggcg
     1021 gaacgaagtt cgcgccggtg ccgttggccg taatcgaagg cgatggcccg gcaattccaa
     1081 cgcttgatcc ggagggccca ataaagcgcc gtggacgaat cgagtccgcc ggaaagcaga
     1141 acgacggccc gggggggggc gtggagaggc gggcggaggg tcgcggggcc tttcacggac
     1201 gaaggggaga ggcggccggg ctcagacgcc gtcgacgcct tccagtcgga tgcgggcgac
     1261 gttcggcagg ttccaaccga ggaaccgatg cccgaggcgt gagaatttta cggggtcgtc
     1321 ggaggaaaaa taatccactc ggccccgggg gccccgggac accaggaggc cgtcctgatc
     1381 caggcggctt cggacggctt tggcggtttc ctcggcggag tcgatcaatt cgaccgtttt
     1441 ggccactcgg cggaggaccc ctttgatcag agggtaatgg gtgcagccca ggaccagggt
     1501 gtcgattttt tttcggagca ggggggccaa ataaagggag gccacccggc gggtgaccgg
     1561 atgatccagc cagccttcct ccaccagggg gacaaagagg gggcacgcgc ggctgaagac
     1621 ccgaatggtg ccgtccaagc gctggacggc gtgttcgtag gcgcggcttt ggaccgtcgc
     1681 ttcggtgccg atgatgccga cccgtccggt gcgcgtggcg gccaaggcgg cccgggcgcc
     1741 gggttcgatg acgccgagga ccgggacggg catgaaggcc cgcagttcgg gcagggccag
     1801 ggccgagacg gtgttgcagg cggtgaccat cattttgacg ttttttcgac gaaaaaagcg
     1861 ggcgacttcg atggagaaac gcttcaccga ttccggggat ttggaaccgt acggaacccg
     1921 ggcggtgtcg cccacgtaga tgaaatgttc ccgggggagg agggagacca gggcccggag
     1981 gacggtgaga ccccccacgc ccgaatcgaa gacggcgatg gggcggggac gcgcgcccat
     2041 caattccccc ccgccgacga ttgggccagg cgcgtttgaa ttttccgttt ttcataatcc
     2101 aggagaccgg cgtaaacggt ctcgatcaat ttttgatgat accgggtcga tcgcaggagg
     2161 ccttcctctt tgggatgggt gatgaatccc gtctcgatca aaatggcggg catggccgtt
     2221 ccccgcaaca cgtaaaaccc ggcttgtttc actccgcgat tggccagggg cagtcgcttg
     2281 gccgcctggt gggcgatcag cgcggcgatt tccgacgaat cgttcatgtg ttcgttgcgc
     2341 gccagggacc agagcaattc ctccactttt tccttggcgg gcccggccac gccttccagg
     2401 tcgacgacgg cgttttcccg gcgcgccacg gcggcggctt cgtcatcggt ggccttttcg
     2461 gaaaggtagt aaatctcgaa cccgttggag gcccgggtca gccccgcgtt gcagtgaagg
     2521 gaaatgaaga gatcggcttt gttcttgttg gccaccgatg agcgttcttc gagcgtcaga
     2581 aagcggtcgt ccgagcgggt cagaaggacg ttgaaccggc cttcggcccg aagcagttgg
     2641 gccagggccc gggccgttcg caagttcacg tctttttcgt aggtcccccc gggccccacg
     2701 gcgccgacgt cttttccccc gtggccggcg tcgatcacga tggtgcgaag gggcgatagg
     2761 gccaaatagg gggcgtccag ggacgaccgg ggggcatcgg ccgggggcgc ccgaaggacg
     2821 ggctcgacct cttcgtcggg cggcggggag cggcgccccg gggaccacac cgcgcctttc
     2881 gggacggcaa tggtgagggt tcgcggagag gcggtggtgt aaatgtcggg cgtgcccgct
     2941 cccgtggcga ggcggacggt gaggtccgtg gtgcgggcgt ggggcgtcat ttcgaccgaa
     3001 gcgaccgtcc cctcgtcaaa agtcaatttt tccgactggg ccgagcggcc gtcgaagagg
     3061 cggaggtaaa gcgtgttgtc ccgttggccg agaagccgat agtccacgtc gggtccgagt
     3121 tcgaccgaga cccgcgtctc ctgggggtaa ttaaaaaccc gggccgacga aatcgatgga
     3181 atcggatcga ccgtcaagga cgggggcgat tcgatccact gaattttcgc tttcgtgaag
     3241 gattggtagg ccggggtcgt cagaagggaa acgggcaaga acgccgagtc cccccaccag
     3301 cgcacggacg tctccagtgc gacggattgg ccgccgacga cggcggtgga actgtccaaa
     3361 acaaattcgg cggtgcggtt ttcgaggcgg tagtcgaatt ttcgagagac ccttttccag
     3421 gtcacccggc cgccgaaaat tttttgcagg tgttcgaggg gcaaacaggc gatgccgtcg
     3481 atgcgaaaca acgacgcggg tccgcgggtc tctccgtcca cgaccaccgt cattgaatcg
     3541 gccggccagg gcggtcgccg ggcggctccc aggagggcga cgccgacgag gacggccagg
     3601 gcggtttttg atgtacgcat ggggcaatta tattaaacat cgacgacctt cgtcggcggg
     3661 gtgctcccgc ccatattgca aaacgggtcg catttgtgta ttgttacacc gggatgttta
     3721 acatgaaaca ttgcgtggag gagccatgga gaaggcaaag agaagtttaa ccgattttga
     3781 gcgtggcgta attatcggat tgttggtggg cgagggacat tttggggggg atggccggca
     3841 accacacatt ctcatcaaaa tgcatgtccg tcaccagcct cttttggaat ggcttggggc
     3901 ccgcataccg ggggcaaagc tttatggtcc ctacaatcat ggaggaaggc actactccca
     3961 attaatggta agggggcggc cattgctgga ttttgttctt ggattgattg ccgattcgcg
     4021 ttggtgggaa attgacccgc attcacacca gcgtcttcgg ggcatgatcg atcggtatat
     4081 ggccccgggg gggtaatagg cgaaatcagc gtttcttaaa aggaataacg aaaagaaaac
     4141 ccgccaggaa gcctccggcg tgggcccacc aagccacacc gcccatttcg gaccccccag
     4201 cggaggcatt taaaatagac ccccagcttt gaagggcttg aagcaaaaac caaaagccaa
     4261 gaaaaaggat ggccggaatt tcaatgaatc gaataaaaat ccagatcgga accaaggccg
     4321 ttattttcgc ctttgggaac aagatgaaat aggcacccat gacgcctgcg atggccccgg
     4381 aggccccgat gagaggcagg gtcgagtggg gattaaggca aacctgggac agggccgcgc
     4441 aggaaccgac gagaaggtaa aatatcaagt accgtcgatg tcccattcgg tcttccacat
     4501 tatccccaaa aatccacagg taaagcatat tcccgagaaa atgcatccag ccgccgtgaa
     4561 ggaacatgga agaaagaatt ttaatccact cggacgccgg gtttctaatc aaattccctg
     4621 gaacgagcga ccactgccct aaaaatgcca tgagggcctt cggggacggt tggcgaatct
     4681 ctaaatagaa aatggcaccg ttgaggagaa ttaacaggat attaacaaag gggaaggaac
     4741 gaccttttat attgtcctta agagggatca tcgtttaatt ttagacagcc cgagtgcggg
     4801 aaaaacactt ggttcaaggt gggatgcttc catccattgc tttttggctt catccaatct
     4861 cttttggccc cttaaaaacc atgcgtagtc ggcccgagct tcggctgaaa aggggtcgat
     4921 ttccaaagct ttaagaaaac acttttcggc ttcgttgttc ttcccctcgg cggaccggga
     4981 aagaccaaga gccagttggg catccacatc gccaccgttc agtcccaccg cccgttccgc
     5041 cacatcgacc gcttcgctcg cccggccgat taatagaaag gcccgggaaa gaagagcttg
     5101 ggccgacgcc agtcgggggt aggcgtccga caccagcttt gcgtccacga cggcgtccga
     5161 cacccgtccc aggtccaaat aagcccatcc ccgggaaaga agggcgcccg ggtgttcccg
     5221 gtacaattca acagtccgat cgatccagcg aagccgatct ttgttgtcgc cccggcgccg
     5281 ggcgatgtcg gccaggagga gcaaaacccc ggcatcgttg gaatccaaat ccaaagccct
     5341 tcgagcgtgg gcgccggcgt ccaccgcgcg atgacaatgg aggtccaatt gggctcgagc
     5401 catctcgtag tcccgctgaa ggccccgggc gtaaaggcgc ccctggaaat ttcgcgccac
     5461 gctgtcccaa tcctggggtt cgctccaatc ggcgttggat cggagggggg ggagtcgccg
     5521 attgatttcc gataaaatgg cttcggaccg ttcctcctcg ccccgaatcc ccaaccagta
     5581 agcgagggaa aaaccagcct gaaatcgatt gtgatccccc tggtaggcgc gcgcgcagag
     5641 atcctccgcc tcggcgcgcc gaccctgttg ggcgcgtacc ccggccagaa gaaggtccgc
     5701 ctcgatgtcc tccggggccc ggcgcaaacg ccgaatggac gcctccgcgg attcgttgtc
     5761 tccggcggcc aaatgcgccc gggttatttt gaaaagggtg tcccggtcct cgggcacgga
     5821 ccgaaggacg gtgccgtagc gagccaaggc tttctggaaa tagccctggt caaaggccag
     5881 atcgccgtac aactccagca actcggaccg cgcggggaac aacttcaatc cttcttccag
     5941 gatttcggtc gcccgggggt aatcgttttg agccaatcgc acgttcgcga gaataagaat
     6001 cgtccgaaaa tcccccgggg ccaaccgccg cgcttcctcc aaataatccg cggcgaaaga
     6061 aagacggtca aaggccaaat tcaaaattcc cagccccagg gcgccggagg ggtcttgcgg
     6121 gaatttttcg tggagaatcc ggcactcttt ctcggcgttc tccaaggcgc cgagtttcag
     6181 ctgtgattca accagaagtt cccgaaggag ttgctcccga ttccgcggcc atgtccggcc
     6241 ggccaaggcg ccgccggcga tgagcgtggc gtcggcggct tcgcctcggt ccagcgcccg
     6301 ctgggcccgg gcccaagggg ttgaacgtcg ccaaacccaa caaccgccgg ccacgagcaa
     6361 ggaaaggagg agaaagaaaa gagtggggcc acgacgaagc attgctctat tttatatgaa
     6421 gtgtccggcc tccaccagat gttctgtagg ccgattaggc gttaatcgta agcactcgga
     6481 gtttggtttg aagagccgac ccacagcctt ctcgattcca agggccaaag ccccatagat
     6541 ttaaagcggt cgtttggcgg gaattcccac tcttcgcggg tagttaaagg aagtttgaga
     6601 caacttatga atacgaacga tgtagcgatc ccgatcgagg cccggtaacc ggtaaggcgt
     6661 caaattcccc aggcgtcctc cgagttcctt aagcgcccgg ccgacgccct cggcctcggt
     6721 ccgcgcctcc accccccggt gaaggaaaca aaaacccccc ggccggacaa agggcagggt
//
LOCUS       DAOQLS010000015         6669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_4806_length_6669_cov_3.978228, whole genome shotgun sequence.
ACCESSION   DAOQLS010000015 DAOQLS010000000
VERSION     DAOQLS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4806_length_6669_cov_3.978228"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="PK362_00520"
     CDS             complement(<1..1510)
                     /locus_tag="PK362_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNC73358.1"
                     /translation="MESPELARRIDAVAIEKTSSTPYSLMVNFSRFSLRPVGRFFMHL
                     LHPLDYVHSPHQSHGRTLRSVLLQISIPLVVVLYAVWSMGARTQTAGLVDHLMFIVLF
                     FLIYSRLLMVRSLFRRMMLSFAFVMGLTTFFLFIVLNFRFFHSWGQMDALKQWDDVFA
                     IWSGIMALLHPFDLLIGLLIPLLLWQLSMLSDRGMSVSIRRPFFLLALLLASYHYKLT
                     IGENRYSEQNPVFYVLRQKFAQMQLSYGYLLNPRRGPRRWKNEDFVAVNESLYKHAKN
                     RSFPFLKTPLPGAKPLPFALESKPNVVLVLLESFRAFESGAYGAAPSFTPNIDRLAKE
                     GVLLRNFYANGAQTIRGEFAIHASYVPNMRGSPVYLNQPKLNVLTLPMVLKKHGYSTY
                     WIGSHPPTFDKKIEFLSRHGVDNFLYSVPAEGRKLGWGAPDRDLFDYALDILTKAKGP
                     VFAEIMTLSGHFPFGDYPTDGQAPPVTGPALYQKYCRALYYTDFALGEFLRKV"
     gene            complement(1541..2344)
                     /locus_tag="PK362_00525"
     CDS             complement(1541..2344)
                     /locus_tag="PK362_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNC73359.1"
                     /translation="MNDVSPFRALLRYRALPLALLGWASTAFAAPVETRNIAGTLTDV
                     TGDVSVSTGTRAPAREGEIGWRAEAGAVLRTGTDGRAEILFDDGTALRMEPNTLLRVQ
                     EAGRVGRFRQFLVQLVEGRLLSNIPKWNRGARARFKVRTPVAVAAVRGTVFVTDASTA
                     TATVAVYDGVVQAGTAGAPPADVAANQQTDLASAGGAPVVRPLSAAMADYRDSVAELF
                     AAHMDELRSDMDAVRQMNETYMENHRNEVEKSMNDARDNLQDLMEGTAP"
     gene            complement(2473..2546)
                     /locus_tag="PK362_00530"
     tRNA            complement(2473..2546)
                     /locus_tag="PK362_00530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2510..2512),aa:Met,seq:cat)
     gene            complement(2626..3399)
                     /locus_tag="PK362_00535"
     CDS             complement(2626..3399)
                     /locus_tag="PK362_00535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009631945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNC73360.1"
                     /translation="MIKWRWLLALTIWGANARAEIKTSLIDYKDGKTALQGYLAYDDA
                     LSGARPGVLVVHEWKGHGDYVRRRADQLAAQGYMVFALDMYGKGVFAKDHAEAAGLSG
                     VYFKDRSLMRHRAQAGLRILQSQPRVDRKRLAAVGYCFGGTTVLEMARAGMPLKGVAC
                     FHGNLTTPTPARPGGLKAKIIVYQGADDQWTAGGIPEFEAEMKAAKADWKLESYPGAV
                     HSFTVKEAGDNPASGMAYNAEADRRSWESLTAFLTGLFK"
     gene            complement(3420..4211)
                     /locus_tag="PK362_00540"
     CDS             complement(3420..4211)
                     /locus_tag="PK362_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC73361.1"
                     /translation="MIFRIGTVVRRYVLIHLRSPARLLDLFFWPVMELLVWGFFTVYL
                     RQNSLDGAGRVMLVLINGLIFWDILFRSQQAVSLAFMEELWTRNVLNLLISPLRAIEW
                     VTGASLYGLLKTGIIVGVLITLATILYAFQLGALGFYFVPLAFNLLLFGYGMGLFTTG
                     LLLRWGHAAEALIWGIPFLIQPFSAIFYPLSVYPRWLKPFCLLLPSTWAMEGMRGAVL
                     TGALDWRSFAWSLGLNAVYTTAFTIFCARMLERGRVTGQLVRMTG"
     gene            complement(4208..4969)
                     /locus_tag="PK362_00545"
     CDS             complement(4208..4969)
                     /locus_tag="PK362_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73362.1"
                     /translation="MTDAVVRVENLVKRYGDVVALGGVSFEVRRGEIFGLLGPNGAGK
                     TTALHILLGVLTPTEGRARLLDRSPVDDRAFLYPRINFSSAYVQMPFNLTARQNLDIY
                     ARLYGVADRPAVIEGLLDKFQLRARADHRTGQLSAGEQTRLNICKSLLNNPELLFLDE
                     PTASLDPDISDLVRRTLKERQEKTGLTIVYTSHNMSEVESLCDRVLFIHRGRKISEGT
                     PGQVRDQFQKESLEKVFIHLARSGDVLEDAGAANP"
     gene            complement(4966..>6669)
                     /locus_tag="PK362_00550"
     CDS             complement(4966..>6669)
                     /locus_tag="PK362_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNC73363.1"
                     /translation="IGEIPDLGLLIDWCVLTGQSLLQLLPLNDVGYDFAPYSAKSSFA
                     LDPMYLSLRDLIGVDTRAFAGAIDGLARLFPRGKRVNYGVKGAKVSLLWDLFRRADKS
                     LPAFERFRAAQRYWLRDDALYKVLKGRFQGRGWEEWDEPFRWRHPAALEQLAKDEADA
                     LLFQEWLQWQLFEQFRAVKRHAGARGVFIMGDLPFLVARDSADVWAHPDYFKLDLSSG
                     APPDLYFAGGQRWGMPPYRWDAMAARDHDYLVEKLRYAENFYHLYRIDHVIGVFRLYT
                     IPLSAPAERGGLDGTFDPPDERQWEDHGTRLLRVMLNATSMLPCGEDLGVVPACSNPT
                     LARLGIPGLDVQRWARDWGTTYDFRDPAQNRKNACAVVSTHDMSNVSAWWEEEAGTVD
                     DYFFRLKCAERRMDADGLRRRLFDAEPAAPGRLRWNPHLRDVPALVRTLERREEDVRD
                     FIDLFLGSHDEKERFWRRLGGAGPAPQKATPAFVRNALESAGRSAAVFSVQLIFDWLS
                     VDRPLPGRPGDYRVNYPGSVGPHNWSVLCPLSLDDHRRWPGNSIIADINRSTDRWPAA
                     R"
BASE COUNT         1395 a         1975 c         2131 g         1168 t
ORIGIN      
        1 ggactttccg gagaaattcc cccagggcaa aatccgtgta atagagcgcg cgacaatatt
       61 tttgataaag agcgggtccc gtcacgggcg gcgcctggcc gtccgttgga taatcgccga
      121 aaggaaagtg accgctcaag gtcatgattt cggcgaaaac cggccctttg gccttcgtta
      181 aaatgtccaa ggcgtaatcg aaaaggtccc ggtccggcgc gccccagccg agtttgcggc
      241 cctccgccgg cacgctgtag aggaaattgt cgacgccgtg ccgggacagg aactcgattt
      301 ttttgtcaaa cgtcggtgga tgggagccga tccaatacgt ggaatacccg tgttttttta
      361 gaaccatcgg gagagtcagc acgttaagct tgggttggtt gaggtaaacg ggcgaccccc
      421 gcatgttggg aacgtaggag gcgtggatgg cgaattcccc ccgaatcgtc tgggcgccgt
      481 tggcgtagaa attgcgtagc aacacccctt ctttggccag tcgatcgatg ttgggcgtga
      541 aggacggcgc ggccccgtaa gcgcccgatt caaaggctcg aaaagactcg agcaaaacga
      601 ggacgacgtt cggcttcgat tccaaagcga agggaagggg cttcgccccg ggaagcgggg
      661 tcttgaggaa aggaaacgac cgatttttcg cgtgtttgta aagggactcg ttgacggcga
      721 caaagtcttc gtttttccat cggcgggggc cccggcgggg gttgagcaaa tagccgtaag
      781 acaactgcat ttgggcgaat ttttgacgca aaacgtagaa cacggggttt tgctccgaat
      841 agcgattttc cccgatggtc aatttgtagt ggtaggaggc cagcagaagg gccagaagga
      901 aaaaaggccg ccgaatggag acgctcattc cccggtcgga cagcatgctc aattgccaaa
      961 ggagtagggg aatgaggagg ccgatcagga ggtcgaaggg gtggagcagg gccataatgc
     1021 cgctccaaat cgcgaacacg tcgtcccatt gctttaaggc gtccatctgg ccccaggaat
     1081 ggaaaaagcg gaaattgagg acgataaaaa ggaaaaaagt cgtgagtccc atcacgaagg
     1141 cgaaactcag catcatccgt cggaacaggc tgcggaccat gagcaaccga ctgtaaatga
     1201 gaaaaaaaag aacgatgaac atgaggtggt cgacgagccc cgccgtttgg gttcgggcgc
     1261 ccatggacca cacggcgtag agtaccacga ccagcggaat gctgatttga aggagaacgg
     1321 accgaagcgt ccgtccgtgg ctttggtggg gcgaatggac gtagtccagg gggtgcaaca
     1381 aatgcatgaa aaaacggccg accggccgaa gggaaaagcg ggagaaattc accatcaaac
     1441 tataaggggt ggacgacgtt ttttcaatgg ccacggcgtc gatgcggcgg gccaattccg
     1501 gggattccac aacccgtgaa ggggggcccc gccgccacgg tcagggggcg gttccttcca
     1561 tcaaatcctg aagattgtcc cgggcgtcgt tcatggattt ttcaacttcg ttccggtggt
     1621 tctccatgta ggtttcgttc atctgccgga ccgcgtccat gtcggagcgg agttcgtcca
     1681 tgtgggcggc aaagagctcg gccacgctgt cgcggtaatc ggccatggcc gccgacagcg
     1741 gacgcacgac cggcgccccg cccgcggagg ccaaatcggt ttgttggttc gcggccacat
     1801 cggccggggg ggcccccgcc gttccggcct gcacgacgcc gtcgtaaacg gccacggtcg
     1861 ccgtcgccgt ggaggcgtcg gtgacaaaaa ccgttccgcg aacggccgcc acggcgaccg
     1921 gggtccggac tttgaagcgg gcccgggccc cgcggttcca tttggggatg ttgctcaaca
     1981 ggcgcccctc caccagttga accagaaatt gccgaaagcg tccaacccgt cccgcttcct
     2041 gtacgcgcag caaggtgttc ggttccatcc gcagcgccgt gccgtcgtca aagaggattt
     2101 ccgcgcgacc gtcggttccg gtgcgaagca ccgcgccggc ctcggctcgc cagccgattt
     2161 ccccttcccg ggccggggcc cgggttccgg tgctgaccga cacgtcgccc gtcacgtccg
     2221 tcaacgtgcc ggcgatgttt cgggtttcaa cgggggcggc gaacgccgtc gaagcccaac
     2281 cgaggagggc caacggcaga gcccggtatc ggaggagtgc gcggaacggg gagacatcgt
     2341 tcatgggatt tcctttcgaa gcgattcgac gaccgtcttg gattatatcc aattcgcgac
     2401 gggactcgcc ggggcgcccc ggcaaaacaa aaaaacccgg tccgcggggc ggaccgggtt
     2461 tttcagcaaa tctagcgggg gacggacttg aaccgtcgac cctgcgctta tgaagcgcat
     2521 gctctaacca gctgagctac cccgccaaaa ccgggattac cgccgggcgc cctgccgacg
     2581 aaaggcggcg ggccccgacg ccgggaatta taccattccc gtcgattatt taaaaagccc
     2641 ggtcaaaaag gccgtcagcg actcccagga ccggcgatcc gcctcggcgt tgtacgccat
     2701 gcccgaggcc ggattgtcgc ccgcttcctt aacggtgaag ctgtggaccg cccccgggta
     2761 cgattccaat ttccagtcgg ctttggcggc tttcatctcc gcttcgaatt ccgggatccc
     2821 ccccgccgtc cattgatcgt ccgccccctg gtagacgatg atcttcgcct tcaacccgcc
     2881 ggggcgggcc ggcgtcggcg tcgtgagatt cccgtgaaaa caggcgaccc ccttcaaggg
     2941 cattcccgcg cgggccattt ccaggaccgt cgttcccccg aagcagtagc cgaccgcggc
     3001 caagcgcttt cgatcgacgc ggggctggga ttgcagaatc cgcaggcccg cttgggcccg
     3061 gtggcgcatg agcgaacggt ctttaaagta tacgcccgac aacccggccg cttcggcgtg
     3121 gtccttggcg aaaacgcctt tgccgtacat gtccagcgcg aaaaccatgt agccctgggc
     3181 ggccagttgg tcggcccggc ggcggacgta atcgccgtgg cctttccatt cgtgcacgac
     3241 gagaacgccc ggccgggccc ccgaaagggc gtcgtcgtag gccagatagc cttgcagggc
     3301 ggttttcccg tccttgtaat cgatcaatga ggttttgatt tccgcgcgcg cgttcgcgcc
     3361 ccagatcgtc aacgccaaca gccaccgcca cttaatcatt cgagcctccc gaggattcct
     3421 catcccgtca tgcggaccaa ttggccggtc acgcggccgc gttccaacat gcgggcgcaa
     3481 aaaatcgtga acgccgtggt gtagacggcg ttcaatccca aggaccaggc gaaggaccgc
     3541 caatcgagcg cgccggtgag aacggcgccc cgcatccctt ccatggccca ggtgctgggg
     3601 agaaggaggc agaagggttt tagccagcgc ggataaaccg acaagggata aaagatcgcc
     3661 gaaaaaggtt ggattaaaaa agggattccc caaatgagcg cttccgccgc gtggccccac
     3721 cggagcaaca acccggtcgt gaacaacccc atcccgtagc cgaacaggag caggttgaaa
     3781 gccagcggaa cgaaataaaa cccgagggcg ccaagttgaa aggcgtagag gatcgtggcc
     3841 agcgtgatca aaacaccgac gatgattccc gttttgagga gcccgtagag ggaagccccc
     3901 gtgacccatt caatggcccg caggggcgaa atcagcaggt ttaaaacgtt gcgggtccac
     3961 aattcctcca taaaggcgag actcaccgcc tgctgggagc gaaagaggat gtcccagaag
     4021 atcaatccgt taatcaggac gagcatgacc cggccggccc cgtccaggga attttgccgc
     4081 aagtagaccg tgaaaaatcc ccagaccaga agttccatga cgggccagaa gaacaagtcc
     4141 agcaagcggg cggggctccg caaatggatc aagacgtagc gccgcacgac cgtgccgatg
     4201 cgaaagatca tgggttcgcc gccccggcgt cttccaggac atcgccggag cgcgccaagt
     4261 gaatgaagac cttctccagg gattcctttt ggaattggtc gcgcacctgg ccgggggtgc
     4321 cttcgctgat tttcctcccc cggtgaatga agaggacgcg atcgcacaag gattccacct
     4381 cggacatgtt gtgggaggtg tagacgatcg tcaggcctgt tttctcctgc cgctctttca
     4441 gcgtccgccg cacgaggtcc gaaatatccg ggtccaggct ggcggtgggc tcgtccaaaa
     4501 aaagcaattc ggggttgttg agaaggctct tgcagatgtt cagccgggtt tgttcgccgg
     4561 ccgacaattg gccggtgcgg tgatcggccc gggcccgaag ttggaatttg tcgaggaggc
     4621 cttcgatgac ggccggccga tcggccacgc cgtagagccg ggcgtagatg tcgaggtttt
     4681 ggcgcgcggt caggttgaag ggcatttgga cgtaggccga ggaaaaattg attcgggggt
     4741 acaaaaacgc ccggtcgtcg accggcgacc ggtcgagaag acgcgcgcgg ccctccgtgg
     4801 gggtcaaaac gcccaggagg atgtggagag cggtggtttt tcccgcgccg ttgggaccca
     4861 aaaggccgaa gatttccccc cgccggacct cgaaagaaac gccgcccagg gcgacgacgt
     4921 cgccgtagcg tttgacgagg ttttcaacac ggacgacggc gtcggtcatc gggcggcggg
     4981 ccaacgatcg gtggatcggt tgatgtccgc gatgatggaa ttgccgggcc accggcggtg
     5041 gtcgtccaag gagagcggac aaaggaccga ccagttgtgc gggccgacgc ttcccgggta
     5101 attgacccgg taatccccgg gacgtccggg cagcggacgg tccaccgaca gccaatcgaa
     5161 aatcaattga acactgaaga ccgccgcgga tcgccccgcg gattccaagg cgttgcgcac
     5221 gaaggccggc gtggcctttt gaggcgcggg gccggcgccg cccagacggc gccaaaaccg
     5281 ctccttttcg tcgtggctgc ccaaaaacag atcgatgaag tcccggacgt cctcctcccg
     5341 gcgttcgagc gtccgcacca gcgcgggcac gtcgcgaagg tgggggttcc accgcagtcg
     5401 gccgggggcc gcgggttcgg cgtcgaacaa acgccgccgt agtccgtcgg cgtccattcg
     5461 gcgttcggcg cattttaggc ggaagaaata atcgtccacc gtcccggctt cctcctccca
     5521 ccaggcggac acgttcgaca tgtcgtgggt gctcacgacg gcgcaggcgt ttttgcggtt
     5581 ttgggccgga tcccgaaaat cgtaggtggt cccccaatcc cgcgcccagc gttggacgtc
     5641 caggccggga atgcccaatc gggcgagggt cgggttcgag caggccggca ccacgccgag
     5701 gtcctccccg caggggagca tggacgtggc gttcaacatc acccgaagca gccgcgttcc
     5761 gtggtcctcc cattggcgtt catcgggcgg gtcaaaggtc ccgtccaacc ccccgcgttc
     5821 cgcgggggcg ctcaacggga tggtgtagag acggaagacg ccgatgacgt ggtcgatccg
     5881 atagaggtgg taaaagtttt cggcgtaacg aagcttttcc accaggtagt cgtggtcgcg
     5941 cgcggccatg gcgtcccatc ggtacggcgg catgccccag cgttgacccc cggcgaagta
     6001 aagatccggc ggggcgccgg aggacaaatc gagtttaaaa taatcggggt gggcccagac
     6061 atcggcactg tcccgggcca ccaaaaaggg cagatcgccc atgataaaaa ccccccgggc
     6121 gccggcgtgg cgtttcaccg cccggaactg ttcaaacaat tgccactgaa gccactcttg
     6181 aaacagcagg gcgtcggctt cgtccttggc caattgttcc agggccgcgg ggtggcgcca
     6241 ccgaaacggt tcgtcccatt cctcccaccc ccggccctga aatcgtcctt ttaaaacttt
     6301 gtacaaggcg tcgtcccgca gccaatagcg ttgggcggcg cggaatcgct cgaaggcggg
     6361 aagggatttg tcggcgcgtc gaaagagatc ccacaggagg gacaccttgg cgcctttgac
     6421 gccgtagttg acccgtttgc cccggggaaa aaggcgggcc aatccgtcga tggcccccgc
     6481 gaaagcccgg gtgtccacgc cgatcaggtc ccgaagggac aaatacatgg ggtccagggc
     6541 aaaggagctt ttggcgctgt agggcgcgaa gtcgtagccg acgtcgttca acgggagcag
     6601 ttgcagaagg ctctggcccg tgagcacgca ccaatcgatc aacaaaccaa ggtccgggat
     6661 ttcgccgat
//
LOCUS       DAOQLS010000016         6541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_4957_length_6541_cov_4.662504, whole genome shotgun sequence.
ACCESSION   DAOQLS010000016 DAOQLS010000000
VERSION     DAOQLS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4957_length_6541_cov_4.662504"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /gene="pyrH"
                     /locus_tag="PK362_00555"
     CDS             <1..277
                     /gene="pyrH"
                     /locus_tag="PK362_00555"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958842.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNC73364.1"
                     /translation="LRASEIEAHVVLKATQVDGVYDSDPRKNPKAKRYKTLSLLTALR
                     DRLGVMDATALSLCLENKIPVRVFNLRGPGNMRRAIAGGDVGTLVTP"
     gene            299..871
                     /gene="frr"
                     /locus_tag="PK362_00560"
                     /pseudo
     CDS             299..871
                     /gene="frr"
                     /locus_tag="PK362_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
     gene            1002..1649
                     /gene="uppS"
                     /locus_tag="PK362_00565"
     CDS             1002..1649
                     /gene="uppS"
                     /locus_tag="PK362_00565"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNC73365.1"
                     /translation="MDGNGRWAKSRGLPRLMGHRAGAESVREIVRAAGELGIGTLTLY
                     AFSTENWQRSPLEVQGLMRLLVHTLRKESQELHKNGVRLHAVGRTDGLPAPVRKELER
                     TIEFLSANRGLHLNLALNYGGRQEIVDAARAVARAGESITEESIARHLYTAHSPDPDL
                     VVRTSGEFRLSNFLLWQSAYAEIHVTPTYWPDFRRRELVMALLDYQRRERRFGAA"
     gene            1652..2503
                     /locus_tag="PK362_00570"
     CDS             1652..2503
                     /locus_tag="PK362_00570"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNC73366.1"
                     /translation="MVLPRVLTALVLGPLFLWVLYLGSIPFLIFMGTLILLGLWEFHS
                     MAEAGGHANQSVTGIAAALLVTLSLLFPGLRGDAPFCAQAPAFSLTLAAALMIFRELG
                     RSDKSLSMLRMAMTCAGVMLIAWPLGGFILLREARGATPELFHAGRSAAFFLVALIWT
                     QDTAAWAVGMTLGKHRLAPQVSPKKSWEGAVGGLLAAVLVSLFLREGWMKSLFGRGET
                     VLVAIVLGILAQASDLAESLFKRCFGVKDSSGLLPGHGGILDRFDSFLLSGPVLYFYL
                     ISVGRIL"
     gene            2503..3690
                     /locus_tag="PK362_00575"
     CDS             2503..3690
                     /locus_tag="PK362_00575"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNC73367.1"
                     /translation="MRRLVLLGSTGSIGVNTLDCVRRLSRAGERWSLRGLSAFSNLDK
                     LKDQIREFRPPRAVVGTDEGAIALRAWLRSRRIACRVDVGVEGLVDLAREASADLVVS
                     AVVGSVGLRPLLAAVQAGKKIALANKEALIVAGDLLMKAARRHGAELLPIDSEHSAIF
                     QCLNGAGPRSVRRLILTASGGAFYRRRGSLDTVTPREALDHPTWKMGKKITVDCATLT
                     NKGLEAIEAHHLFQVPLDRIDIVVHPQSIVHSLVEFNDGAVLAQLSHPDMRLPIQYAL
                     THPERRPTPLKTLRLEEMGRLDFHRPDFGRFPSLSLALAAGRRGGTWPAVFNGANEVA
                     VHAFLNGELSFTGIPTLCRRVLTAHRPASRTPGEDAGLSAILRADSWAREKARTLIDR
                     EKK"
     gene            3687..4784
                     /locus_tag="PK362_00580"
     CDS             3687..4784
                     /locus_tag="PK362_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNC73368.1"
                     /translation="MISFLMGSASVLIAFGLVIFVHEFGHFIVAKKSGVKVDRFSFGL
                     GPELFGFTWGETRYCVAWIPLGGEVRMAGEMDPGAERAAPRDPREFFAKPWYRRIPIV
                     VAGPAMNYLLAFLLFSLVFFAWGNPSLSPDAVVGDVAEGFPAQSAGLRPGDRVLSIDG
                     LAVAQWKDLAQAIHERADRPVTLEVRRAGTDPAAAAVRVQLTPRRDPATGHGLIGITP
                     LTVYEKMGLWASFRQGGFQTVFWSLHTLDYLKDRLVRREKPELSGPVGIAAVISKSAR
                     SGLQDYIFLIAMISLGIGLFNLFPIPMLDGGHLMFYLIEGLVRRPVSRRVVQTANAVG
                     LSVLLGILVFATYSDIQRLRGGDAPAATGNK"
     gene            4784..6505
                     /locus_tag="PK362_00585"
     CDS             4784..6505
                     /locus_tag="PK362_00585"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNC73369.1"
                     /translation="MRLSRYLLPTLKELPSDADTPSARLMLRSGMIRKVASGLYEWLP
                     FGLRALRNVERIVREEMDAAGGQEVWLPTLQPKELWMESGRWQAYGKEMMRLKDRKDG
                     EFCLAPTAEEVITDLVRREVRSYRELPMLLYQFGEKFRDEIRPRFGVMRAREFYMKDA
                     YSFHADEVDLEKTYRAVFAAYERVFTRCGLKFRPVEAQTGAIGGNFSHEFMVLAETGE
                     ETIAACDECGYAANVERAECLAPAADGETPRPMEEVDTPGLGAVADVAKFLNLPESKF
                     LKTQVYVADDKPVIALLRGDTELNEAKLQKVLEARVLYRAGDDVYRSVAGCDVGYAGP
                     QGRSVPVIVDLAAAAVVNGVSGANKNGRHVKNVNAPRDYTAARVADLRLVRESDPCPR
                     CGKRLSFFKGIEVGHTFKLGTKYSQAMGAGYLTEKGAKTPFQMGCYGIGVSRVVAAAL
                     EQCHDDNGIVWPEAIAPFDVAILPLNVSEPRLMETAERLEKELTARGFQVLLDDRDQR
                     AGVKFKDADLLGLPWRVTVGEKKLAVGQVEIKRRGAADASDVAIETAAAWLADRRGRK
                     SPEAVPA"
BASE COUNT         1185 a         2105 c         2012 g         1239 t
ORIGIN      
        1 cctgcgagct tcggaaatcg aggcgcacgt cgttctgaag gccacccaag tcgacggggt
       61 ttacgattcg gacccgcgca aaaacccgaa agccaaacgc tacaaaacgt tgtcgctctt
      121 gaccgccctt cgggaccgct tgggcgtcat ggacgccacc gccctttccc tttgtttgga
      181 gaacaagatt cccgttcgcg tctttaatct tcggggcccc ggcaacatgc gccgggccat
      241 cgccgggggc gacgtgggta ctcttgtgac gccctgaccc gaaaccccga ggcctctcat
      301 gccgtccctc aacgccgtcg cgcaaccgtt catcaacgaa gccgaagaaa aaatgaaaaa
      361 gtcggtggat aaaatccgcc aggaatacgc gagccttcga accggccgcg ccaccggctc
      421 cctcctggac catttgaagg tggaatacta cggctcccac gtcccgctca aacaggtcgc
      481 ggccgtgagc gtccccgaag ggcgcaccct ggaagtgaaa ccctgggaca tcggcgccct
      541 ggccgcgatt gaaaaagcga ttcgcacctc ggacctcggg ctcaatccca ccaacgacgg
      601 aaagatgctg cgcttgaaca ttccgacttt gaccgaggaa cggcgaaaag aaatggtcaa
      661 gcacgtcaaa aaagtggccg aggacttccg cgtgtccatt cgaaacgacc gccgcgacgc
      721 catggaaaaa atcaagaaag cggagaaaga caaaaccctt tccgaagacg accgcaaaaa
      781 cgcggaacac gcgctccagc acgtgaccga cctttacatt aagaaaatcg acgaaaccct
      841 cgcgatcaaa gaaaaagaca ttctggagat ttgcccgggc cgtcgacccc atgcccactc
      901 tcgcgaagcg ccgccccgcc gtgcccaccg gcgagccctc cctggacgct ttggaagccc
      961 gcctggaccg cgcgcggatc ccgcggcacg tggccgttat catggacggc aacgggcgtt
     1021 gggccaaaag ccgcggcctg ccccggttga tgggccaccg ggccggcgcc gagagcgtcc
     1081 gtgaaatcgt ccgcgccgcg ggcgaattgg gcatcgggac cctcaccctt tacgcctttt
     1141 ccaccgaaaa ctggcaacgg tctcccctgg aagtccaagg tctcatgcgc ctgctggtcc
     1201 acaccctccg caaagaatcc caggaacttc acaaaaacgg tgtgcggttg cacgccgtgg
     1261 gccgcaccga cggcctgccc gccccggttc gaaaggaact cgaacgaacc atcgaatttc
     1321 tgtccgcgaa tcgtggcttg catctgaatc tcgcgctcaa ttacggggga cgacaggaaa
     1381 ttgtcgacgc ggcccgggcc gtggcgcggg cgggggaaag catcaccgag gaatccatcg
     1441 cgcgccacct gtacaccgct cattccccgg accccgactt ggtggtgcgg acgtcgggag
     1501 aatttcgtct gtccaacttc cttttgtggc agtcggccta cgccgaaatc cacgtcacgc
     1561 cgacctactg gcccgacttc cgtcgacgcg aattggtgat ggctctgttg gattaccagc
     1621 ggcgggaacg ccgtttcggg gcggcctgac cgtggttctt ccgcgcgtcc tcacggcgtt
     1681 ggtcctgggg cccctcttcc tttgggtcct ctatttggga agcattccgt ttctgatctt
     1741 tatgggcacg ctgatcctgc tgggcctttg ggagtttcac tccatggccg aggcgggcgg
     1801 tcacgccaat caatcggtca cgggaatcgc cgcggccctc ctggtcaccc tgtccctcct
     1861 gtttccgggc ctgcggggcg acgccccctt ttgcgcccag gcgccggcct tttccctgac
     1921 cctcgccgcg gccctcatga tcttccggga attggggcgg tcggacaaaa gcctgtccat
     1981 gttgcgaatg gcgatgacct gcgcgggcgt gatgttaatc gcctggcccc tgggcgggtt
     2041 catccttttg cgcgaagccc ggggcgccac gccggaactg ttccacgccg gacggtccgc
     2101 cgcttttttc ctcgtggcct tgatttggac ccaagacacc gccgcctggg ccgtgggcat
     2161 gacgctggga aaacaccgcc tggcccccca ggtgagcccg aaaaagtcct gggaaggcgc
     2221 cgtgggaggc ctcctggcgg cggtcctggt ttccctcttt ctccgggaag gttggatgaa
     2281 atcccttttc ggccgggggg aaaccgtgct ggtggcgatc gtgcttggaa ttctcgccca
     2341 ggcctcggac ctggcggaat ccctttttaa gcggtgtttc ggggtcaagg attcttcggg
     2401 cctgctcccg ggccacgggg gaatactcga ccgcttcgac tcctttctgc tctcgggacc
     2461 ggtcctttat ttctacctga tctccgtcgg ccggattctt tgatgcgccg cctggttctt
     2521 ttggggtcga cgggatccat cggcgtcaat accttggact gcgtgcgtcg cctgagccgc
     2581 gccggcgaac gctggtccct tcgggggctc tcggccttct ccaacctcga taaactcaaa
     2641 gaccaaatcc gggaatttcg gccgccgcgg gccgtggtgg gcaccgacga aggggccatc
     2701 gccctgcggg cgtggcttcg gtcccgacgg attgcgtgcc gggtggacgt cggggtggag
     2761 gggttggtcg atttggcccg cgaggcgtcc gccgatttgg tggtttcggc ggtggtcggt
     2821 tccgtgggtc ttcggccgct cttggccgcc gttcaagcgg ggaaaaaaat cgccctggcg
     2881 aacaaagagg ccctcatcgt cgccggcgat ttattgatga aggccgctcg acggcacggc
     2941 gcggagcttc tgcccatcga cagcgaacat tcggccatat ttcaatgcct gaacggcgcc
     3001 gggccgcggt ccgtacggcg actgatcctc acggcttccg gcggcgcttt ttaccgtcga
     3061 agagggtcgt tggacaccgt cacgccccgg gaagccctcg accacccgac ctggaaaatg
     3121 ggaaagaaga tcaccgtcga ttgcgccacc ctgacgaaca aaggcctgga ggccatcgaa
     3181 gcccatcact tgtttcaggt gccgctggac cgcatcgaca tcgtcgtcca tccccaatcc
     3241 atcgtgcatt ccctcgtgga attcaacgac ggcgccgtct tggcccaatt gtcccatccg
     3301 gacatgcgcc tcccgattca atacgccctg acccaccccg aacgtcggcc gacgcccctg
     3361 aagacgcttc gattggagga aatgggtcgg ctggacttcc accggcccga ctttggccgt
     3421 ttcccgagcc tttccctcgc cctcgcggcc ggccgtcggg ggggaacctg gcccgcggtt
     3481 tttaacggcg ccaacgaagt ggccgtccac gcctttttaa acggcgaatt gtccttcacc
     3541 ggaattccga ctttgtgccg gcgggtcctc accgcccacc ggcccgcgtc ccgaacccct
     3601 ggcgaagacg ccgggttatc cgctattctt agagccgatt cctgggcccg ggaaaaagcc
     3661 cggaccctga tcgaccggga gaaaaaatga tctctttctt gatgggctcc gccagcgtgt
     3721 tgatcgcctt cggtctcgtg atttttgtcc acgagttcgg ccacttcatc gtcgcgaaga
     3781 aatccggcgt gaaagtggat cgtttttcct ttggcctcgg ccccgaactt ttcggcttta
     3841 cctggggaga aacgcgttac tgcgtggcct ggattcccct gggcggggag gtccggatgg
     3901 cgggggaaat ggaccccggc gcggaacggg cggcgccccg ggatccccgg gaatttttcg
     3961 ccaaaccgtg gtaccgccgg attcccattg tcgtcgccgg gcccgcgatg aattacctct
     4021 tggcgttttt gcttttcagc ctggttttct tcgcctgggg aaacccgagc ctgtccccgg
     4081 acgcggtcgt gggcgacgtg gccgagggtt ttcccgccca atccgccggg cttcgcccgg
     4141 gagaccgggt gctgtccatc gacggcctcg ccgtggccca atggaaggat ttggcccagg
     4201 ccattcacga acgcgccgac cggcccgtga cgctggaggt gcggcgggcc ggaacggacc
     4261 cggcggccgc ggcggtccgg gttcaattga ccccgcgccg ggacccggcc acggggcacg
     4321 ggttgatcgg catcacgcct ctcacggtct atgagaagat ggggctgtgg gcctcctttc
     4381 gccagggggg gttccaaacc gtcttctgga gtcttcacac cctggattac ctcaaggacc
     4441 gcttggttcg ccgggaaaaa ccggagctct ccggccccgt cggcatcgcg gcggtgattt
     4501 ccaaatccgc ccgcagcggg cttcaggact atattttcct catcgccatg atttccctgg
     4561 gcatcggcct gttcaacttg tttcccatcc ccatgttgga cggcggccat ttgatgttct
     4621 atctgatcga aggcctcgta cggcggcccg tcagccgccg ggtggttcaa accgccaacg
     4681 ccgtgggcct ctcggttctt ctgggcattt tggttttcgc cacctattcg gacatccaac
     4741 gcctgcgggg cggcgacgcg cccgcggcca ccggaaacaa ataatgcgcc tttcccgcta
     4801 tctactgccc acgctgaaag aattgccatc ggacgcggat accccctcgg cgcgcctgat
     4861 gcttcggtcg ggcatgatcc gaaaggtggc gtcgggcctg tacgaatggt tgcctttcgg
     4921 ccttcgcgcc cttcggaacg tcgaacgcat tgtccgggag gaaatggacg cggcgggggg
     4981 ccaggaagtc tggctcccaa cgctgcaacc caaggaattg tggatggagt cgggccgctg
     5041 gcaggcctac ggcaaggaaa tgatgcggct caaagaccgc aaagacggtg agttttgcct
     5101 ggcccccacc gccgaagaag tgatcaccga cctcgttcgg cgggaagtcc ggtcgtaccg
     5161 ggaactcccg atgctgcttt atcaattcgg cgaaaaattc cgggatgaaa tccggccccg
     5221 tttcggcgtc atgcgcgcgc gggagttcta catgaaggac gcctactctt tccacgccga
     5281 cgaagttgat ttggagaaaa cctaccgggc ggttttcgcc gcctacgagc gcgtgttcac
     5341 ccgctgcggc ctcaaattcc ggcccgtgga agcccaaacg ggggccatcg gcggcaattt
     5401 ctcgcacgaa ttcatggtgt tggccgaaac cggcgaggaa accatcgccg cctgcgacga
     5461 atgcggctac gccgccaacg tcgaacgggc cgaatgcctg gcccccgccg ccgacgggga
     5521 aaccccccgg ccgatggaag aagtcgacac gccgggcctg ggcgcggtgg ccgacgtcgc
     5581 caaattcctg aacctgccgg agtccaaatt cctcaaaacc caggtgtatg tggccgacga
     5641 caaacccgtc atcgcgctcc ttcggggaga cacggaattg aacgaagcca aattgcaaaa
     5701 agtcctggag gcccgggtcc tttatcgcgc gggggacgac gtgtaccgaa gcgtggccgg
     5761 ctgcgatgtc gggtacgcgg gcccccaggg acggtccgtg ccggtgatcg tcgatctggc
     5821 cgccgcggcc gtcgtgaacg gcgtgtcggg cgccaacaaa aatggacgcc acgtcaaaaa
     5881 cgtgaacgcc ccccgggatt acaccgccgc gcgggtggcc gatcttcgcc tcgtccggga
     5941 atcggaccct tgcccccgct gcggaaagcg gctatccttc ttcaaaggca tcgaggtcgg
     6001 ccacaccttc aaactgggga caaagtactc ccaagccatg ggcgcgggtt acttgaccga
     6061 aaaaggcgcc aaaacaccct ttcaaatggg ctgttacgga atcggcgtca gccgcgtggt
     6121 cgcggcggcc ctggaacaat gccacgacga caacggaatc gtgtggcccg aagcgatcgc
     6181 gccctttgac gtggcgatcc tgccgttgaa tgtttcggag ccccggttga tggaaaccgc
     6241 ggagcgcctt gagaaggaac tgaccgcccg gggttttcag gtgttgttgg acgatcggga
     6301 ccaacgcgcg ggggtcaaat tcaaagacgc ggatttgctg ggcctcccgt ggcgcgtcac
     6361 ggtgggggag aaaaaattgg ccgtgggcca ggtggaaatc aagcggcggg gggcggccga
     6421 cgccagcgac gttgccatcg aaacggcggc cgcgtggctg gccgatcggc ggggtcgaaa
     6481 atcccccgaa gcggttcccg cttaaatcac gacggaggaa aacggtgcct tttgacgaca
     6541 c
//
LOCUS       DAOQLS010000017         6453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5062_length_6453_cov_4.685370, whole genome shotgun sequence.
ACCESSION   DAOQLS010000017 DAOQLS010000000
VERSION     DAOQLS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_6453_cov_4.685370"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..550)
                     /locus_tag="PK362_00590"
     CDS             complement(29..550)
                     /locus_tag="PK362_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73370.1"
                     /translation="MKSRRRSRSPRGFTFVEVLVTLAIVGILFGVIPQIIMQVVRFYN
                     LSMDRLALQRDARIVTEIVVSNLRKAVSTTVRIDQVDVSTQPPYSRIRFETIKGSTVT
                     VWQENNKLYVTDSVVSPLPRVVTPYLRYLAFTYVGTMDDSVMSVNLCLEKESFRKKRI
                     FYVTNDRVRILNE"
     gene            complement(547..>2254)
                     /locus_tag="PK362_00595"
     CDS             complement(547..>2254)
                     /locus_tag="PK362_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73371.1"
                     /translation="YPIQYNGGGGYLRLTYNPTSSTDYAYLDGVGWRGGVDQGGGCVS
                     PAEPVNAEGGVSWRLIDQLGGGVQDCEGLNAGEQWIRYSSTMAWQETRDIGPAYDSDA
                     SVRDWSKWDPGAPGYFARFLPRNSTCPTVPVVSGRDPYQRADRLGNGSEAGYVTAFVN
                     DGISYWVDGIKPTYYTGWTQYGAFCTTCPRSNMYRYTDYDIPWWGRYEFTRVSTGNWT
                     LGMYARRKTGPYDYGVSTQVLLNVTQNSYTWWQGVIASTSDKVFLFGAVRDVYNVPLE
                     DVRVDYKFGDGSTPINIKSRSGGAYSLELSSSVGKVMSGVHLNMPGTGDYLPAYVDVL
                     WGPHEWWGGVGYKVDSCNCPDTHLGYTGFLTGFVQDELGVGIPDVNVSAINAATNEER
                     ASGLTSSGGGRPVGTFLVNVSTGMYKVKVMTELEETSSPTISGLISLATATMSSGGIF
                     VGTFTITNAYGRRAGAVVASGGPLNTGAVLVLTTSTIAGTRPPPIDSVFRAGNVTYYM
                     ASSDAIGRYSFDVKMNTYNLYGFYPYYDASGVLTWVKRSTTTYVNSGNNVLDFPTLDL
                     TP"
     assembly_gap    2255..2354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2355..3424)
                     /locus_tag="PK362_00600"
     CDS             complement(<2355..3424)
                     /locus_tag="PK362_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNC73372.1"
                     /translation="MTYSMGNNKGVTMTELIVAAVLLAVGIFGSLKVFRGISQSIFMS
                     QTKQVATTLAREKLEALQVLPYYRLRAQGAPTYYQPGGSGKPYIYYDPYYTPGPETVQ
                     VGTVKYYRGAYVDRVYRDPSSGDLVAMAVATPYQTEAGLKRIKVGVMWKDGSVWKVVD
                     VTGLKENPVRTRGEVKFVGTVTSTAGIPLSGAANGIVIDTIETLGQMITVQPNGSGYY
                     EFAVRPGTYTIRARDVSSGQVYYTVKQTGYVGYDHNPSTVNFVLPMSLYGRVDTTFWW
                     NSNPVISRVVGSSVSATDPNFNQEWVEIFNPTTWTWDATKLNLHYYMTRNSSESYVNS
                     TAEFAKHKIDANYINTTIPPGG"
     gene            3727..4305
                     /locus_tag="PK362_00605"
     CDS             3727..4305
                     /locus_tag="PK362_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC73373.1"
                     /translation="MDEARKQEFKTKALPLVDEMYGVALRMTRNPQTAEDLVAEAFSR
                     AWKNLTQFQPGTNLRAWMYRIMTNAYINDFRKKNREPEKVSVDAYEKLDEFHLFNRLS
                     SEAPAAPDPVRDVIGRLTNEDFRAALDALPEEYRTAIVLYDLQGLSYDDVAKALDIPI
                     GTVRSRLARGRRQLQGALYRHAVDAGLVEAQR"
     gene            4302..4535
                     /locus_tag="PK362_00610"
     CDS             4302..4535
                     /locus_tag="PK362_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNC73374.1"
                     /translation="MTDHECDKILDHAYEFLNRRDVTPAEEAAFRKHLSDCPPCGDKF
                     EFERKLLERLKAAGGCQCPEGLKARVQSLLERI"
     gene            4543..6006
                     /locus_tag="PK362_00615"
     CDS             4543..6006
                     /locus_tag="PK362_00615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC73375.1"
                     /translation="MFPVAALTAYLSILAVLSLYGFHRYWILFLYWRHYKSRAPLPTP
                     PDPIEWPRVTVQLPLYNEFYVVERLLASVAALDYPSNKLQIQVLDDSTDESRALAERL
                     VAEHRGRGLEIQHVARSDRRGFKAGALANGLKSATGEFVAIFDADFLPPADFLRRTLP
                     WFVDPGLGMVQARWGHVNTGYSLLTRLQALLLDGHFLLEHTARNRSGAFFNFNGTAGV
                     WRRRTIEEAGGWADDTLTEDLDISYRAQMIGWRFLFLPELVCPAELPVDMGAFRNQQH
                     RWTKGALQVAKKILPALWKSPAPFFVKLESTVHLTSNLAYPLVLLFSLLLFPSLVARR
                     ALGWPLWVGAIELTAFALTTASLLLFYSVAQREAFGGPLRLRAKDLPALMAFGVGMCV
                     NNGRAVFEAFFNVSTEFRRTAKFNIRKPGEAWLAKKYRSARPVGALELMVGGYLAAAM
                     AWSLRAGYYWSLPFLSIFLAGYLYVGFLSLRHAFQRA"
     gene            6016..>6453
                     /locus_tag="PK362_00620"
     CDS             6016..>6453
                     /locus_tag="PK362_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73376.1"
                     /translation="MPALPALALALEGVWIASLGLRPWRAHPGGFLAVFAAAFFLYLP
                     AVRRTLKRPQSLGLILAAGVLFRLTAFLSPPSLSDDIYRYLWDGRVQAAGHNPYRFAP
                     DAPELASLRNAEHTGINHKSVPTIYPPLAQAVFRLAVALSPTLA"
BASE COUNT         1170 a         1989 c         1879 g         1315 t
ORIGIN      
        1 ggcgcggctc cgccggctca acgcttcatt attcattgag aatccggacc cggtcgttgg
       61 tcacataaaa aattcgtttt ttgcggaagg attccttttc caggcaaagg ttgacggaca
      121 tcacgctgtc gtccatggtc ccaacgtagg taaaggccaa ataccgcaag tagggggtca
      181 ccacccgggg cagaggggac acgaccgagt cggtgacata caacttgttg ttttcctgcc
      241 aaaccgtcac ggtcgaccct ttgatggtct caaaccggat gcgggaatag gggggttgcg
      301 tcgacacgtc cacctggtcg atccgcaccg tggtggaaac ggcctttcga agattggaga
      361 cgacgatctc cgtcacgatg cgggcgtccc gctggagggc cagccggtcc atgctcaggt
      421 tgtaaaagcg gaccacctgc atgatgattt ggggaataac cccgaagagg atgccaacga
      481 tggccagggt gaccaacacc tcgacaaagg tgaacccccg gggggaccgg gaacgacgcc
      541 gcgatttcat ggcgtcagat ccaacgtcgg gaaatcgagg acgttattgc cggaattcac
      601 gtaggtggtc gtactgcgct tcacccaggt caacaccccc gacgcgtcgt aatagggata
      661 gaagccatac aagttgtagg tgttcatttt cacgtcgaaa ctgtagcgac cgatggcgtc
      721 cgaagaggcc atgtagtacg tcacgttgcc cgcccgaaaa accgagtcga tggggggcgg
      781 tctcgtgccg gcgatggtgg aggtggtcag cacaagcacg gcgcccgtat tcagaggacc
      841 gccgctggcc acgacggcgc ccgcccggcg gccgtaggcg ttggtgatcg taaaggttcc
      901 cacaaaaatc cccccgctgg acatggtcgc ggtggcgaga ctgatgaggc ccgaaatcgt
      961 cggggacgag gtttcctcca attccgtcat cactttgact ttgtacatgc ccgtggacac
     1021 attaacgaga aaggtcccca cgggccgtcc cccgccggag gacgtaagac ccgaggcccg
     1081 ttcttcgttg gtcgccgcgt tgatggcgct gacgttcaca tcggggattc ccacccccaa
     1141 ttcgtcttga acgaacccgg tcaaaaagcc ggtgtatccc aaatgcgtgt ccgggcagtt
     1201 gcaggaatcc accttgtagc cgaccccgcc ccaccactcg tgggggcccc aaagcacatc
     1261 cacgtaagcc ggaaggtagt cgcccgtgcc gggcatattg agatgaaccc ccgacatcac
     1321 ctttcccacc gaggaagaga gttccagact gtacgctccc cccgagcggg acttgatgtt
     1381 aatcggcgtc gaaccatcgc cgaacttgta atcgacgcgg acgtcttcga gcggaacgtt
     1441 atacacatcg cggacggccc cgaacaggaa caccttgtcg gaggtggacg cgataacgcc
     1501 ttgccaccag gtgtaactgt tttgagtgac gttcaacaac acctgggtgg agaccccgta
     1561 atcgtagggg cccgtttttc ggcgggcgta catgcccagg gtccagttcc ccgtggacac
     1621 ccgtgtgaat tcgtatcgac cccaccaggg gatgtcgtag tccgtgtatc gatacatatt
     1681 ggatcggggg cacgtggtgc aaaaggcgcc gtattgggtc cagcccgtgt agtacgtcgg
     1741 cttgattccg tcgacccagt acgaaatgcc gtcgttcaca aacgccgtca cataccccgc
     1801 ttcgctgccg ttccccaggc gatcggcccg ttggtaggga tcccgccccg acaccaccgg
     1861 aacggtggga caggtgctgt ttcgggggag aaagcgggcg aaatagcccg gggcccccgg
     1921 gtcccatttg gaccaatccc ggacgctggc gtcggaatcg taggcgggtc cgatgtcccg
     1981 ggtttcctgc caggccatgg tgctggagta acgaatccac tgctcaccgg cgttgagtcc
     2041 ttcacaatcc tgaacgccgc cgcccagttg atcgatcaat cgccaggaaa caccgccttc
     2101 ggcgttgacc ggttcggcgg ggctcacgca cccgccgccc tggtccaccc cgccccgcca
     2161 ccccacgccg tccaggtagg cgtaatcggt cgacgaggtg ggattgtacg tgagccgcag
     2221 gtagccgccg cccccgttgt attgaatggg gtagnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnntaaccg ccgggcggaa tcgtggtgtt gatgtaattg gcgtcgattt
     2401 tgtgtttggc gaactcggcg gtggaattga cgtaggattc ggaactgttc cgggtcatgt
     2461 aatagtgaag gttcagtttg gtcgcgtccc aggtccacgt cgtgggatta aagatttcca
     2521 cccattcctg gttgaagttg ggatccgtgg cgctcacgga actgcccacg acccggctga
     2581 tcaccgggtt cgaattccac caaaaggtgg tgtccacccg cccgtaaagg ctcatgggaa
     2641 gaacgaaatt gacggtggac ggattgtggt cgtatcccac gtaaccggtc tgtttgacgg
     2701 tgtaatacac ctggccgctg ctcacgtccc gggcccgaat cgtgtaggtc cccggacgaa
     2761 ccgcgaattc gtaatagccc gatccgttcg gttggacggt tatcatttgg cccagcgtct
     2821 cgatggtgtc gatcacgatg ccgttggcgg ccccggaaag cgggattccg gccgtggagg
     2881 tgacggtgcc gacgaattta acctcgcccc gggtgcgaac ggggttttcc ttgagtcccg
     2941 tcacatcgac caccttccac accgacccgt ccttccacat gacgcccact ttgatccgtt
     3001 tcagtccggc ttccgtctga tagggcgtgg ccacggccat ggcgaccaga tcgccggaag
     3061 aaggatcccg gtaaacgcgg tcgacgtagg ccccgcggta gtatttgacc gtgccgacct
     3121 ggaccgtttc cgggcccggc gtgtaatagg ggtcgtaata aatatacggt ttcccgcttc
     3181 cgccgggctg gtaataggtc ggcgcgccct gggcgcggag tcgataatag gggagcactt
     3241 gaagggcttc cagtttttcg cgagccaagg tcgtggccac ttgtttggtt tggctcatga
     3301 aaatggattg ggaaatgccg cggaacacct tgagggagcc gaaaattccg accgcgagca
     3361 atacggcggc cacgatcaac tccgtcatcg tgacgccttt gttgttgccc atcgaatagg
     3421 tcatgttatc cccgcacttg gactttggga tcgagagaaa aaagcccggt ggtcccgtta
     3481 agtatacatc atgaaatccc gggccatcga gtctttccct tgattgccat gcgtcggaat
     3541 ggccaaaccc gttctttccc tcttttgaag gccttttttt gatttttccg ccctttcgac
     3601 cccgccgccg tttcggagcc gggtcaaccc ccttgacaag gcggggcgga accggatttg
     3661 cggggtccgt cggtaattga taatcttgaa tcggcgcggg aacttttttc cccgtcgggg
     3721 tttagaatgg acgaggcacg gaaacaggaa ttcaaaacca aggcgctccc cctggtggac
     3781 gaaatgtacg gggtggccct tcgcatgacc cggaatcccc agacggccga agacctggtg
     3841 gccgaggctt tttcccgggc gtggaaaaac ctgacccaat tccaaccggg gaccaatttg
     3901 cgggcgtgga tgtatcggat catgaccaac gcctacatca acgattttcg gaagaaaaac
     3961 cgggaaccgg agaaggtgtc cgtcgacgct tacgagaaat tggacgagtt ccaccttttc
     4021 aaccgactgt cgtcggaagc tccggcggcc ccggaccccg tgcgggacgt catcggccgg
     4081 ctgaccaacg aggattttcg cgcggccctg gacgctttgc cggaagagta ccggacggcg
     4141 attgtgcttt acgatctcca ggggctttcc tacgacgacg tggccaaggc cctggacatc
     4201 ccgatcggaa ccgtgcggtc gcgcctggcc cggggccggc gacaacttca gggggcgctc
     4261 taccgccacg cggtggacgc cggattggtg gaggctcaac gatgaccgac cacgaatgcg
     4321 ataaaattct ggaccacgcg tacgaatttc tcaatcggcg ggacgtgacc cccgccgagg
     4381 aggccgcctt tcggaagcac ctgagcgact gccccccctg cggcgacaaa ttcgaattcg
     4441 agcggaaatt gctggagcgg ctcaaagccg ccggcgggtg tcaatgcccc gaggggttga
     4501 aagcccgggt ccagtccctt ctggaacgga tttaatccct tcgtgttccc cgtcgcggcc
     4561 ctcaccgcct acctgagcat cctcgccgtt ctttcccttt acggcttcca tcgctactgg
     4621 atcttgttcc tctattggcg ccattataaa tcccgcgccc cgctgccgac gccgccggac
     4681 cccatcgaat ggccccgggt gacggtacag ctcccgttgt acaacgagtt ctacgtggtc
     4741 gagcgcctgt tggcgtccgt ggccgcgctg gactatccgt cgaacaaact tcaaatccag
     4801 gttttggacg actccaccga cgagagccgg gcgttggccg aacggctcgt ggccgaacac
     4861 cggggacggg gcctggaaat ccaacacgtg gcccggagcg accggcgggg gttcaaggcc
     4921 ggcgccctgg ccaacggact gaaaagcgcc acgggcgaat tcgtggccat ctttgacgcg
     4981 gatttcctcc ccccggcgga tttcctccgc cggacccttc cgtggttcgt cgatcccggc
     5041 ctcggcatgg tgcaggcccg gtggggccac gtgaacacgg gctactccct ccttacccga
     5101 cttcaggctc tcctgttgga cggccatttt ctcctggaac acacggcccg aaatcgttcg
     5161 ggcgcctttt tcaacttcaa cgggaccgcc ggggtttggc gccgccggac catcgaagaa
     5221 gccggcggct gggccgacga taccctcacc gaggacctcg acatctccta tcgggcccaa
     5281 atgattggtt ggcgattcct ctttttgccc gaattggtct gcccggcgga gctcccggtc
     5341 gacatggggg ccttccgcaa ccagcaacac cgctggacca agggcgccct tcaggtggcc
     5401 aaaaaaattc tccccgctct ctggaaaagc cccgcgcctt tttttgtcaa attggaaagc
     5461 accgtgcatt tgacgtccaa cctcgcctac cccctggtgc tcctcttttc cctccttctc
     5521 tttccaagcc tggtggcccg gcgggccctg gggtggcccc tctgggtggg ggcgatcgaa
     5581 ttgacggcct tcgcgctgac gaccgcgtcc ctcctgcttt tttactcggt ggcccaacgg
     5641 gaagccttcg gcgggcccct gcggctgcgg gccaaggatt tgcccgccct catggctttc
     5701 ggcgtcggca tgtgcgtcaa caacggccgg gcggtgttcg aagcgttctt caacgtttcg
     5761 accgaatttc gccgcaccgc caaattcaat attcgaaaac ccggcgaggc ctggctcgcc
     5821 aaaaaatacc gttccgcccg acccgtcggg gccctcgaac tgatggtggg cggctacctg
     5881 gccgccgcca tggcctggtc ccttcgggcg ggttattact ggtccctgcc gtttctttcg
     5941 attttcctgg ccggctatct ttacgtcggc tttctcagcc ttcggcacgc ctttcaacgg
     6001 gcctgaaacc cgcccgtgcc cgcgcttccc gccctggccc tggccctgga gggggtttgg
     6061 atcgcctccc tgggtctccg cccctggcgc gcccaccccg gtggtttttt ggccgtcttt
     6121 gccgcggctt ttttcctcta tctcccggcg gtccgccgga cgctgaaacg ccctcaatcc
     6181 ctgggtctga ttttggcggc gggcgtctta ttccgattga ccgcctttct ctccccgccc
     6241 agcctttcgg acgatattta ccgttatctg tgggacggtc gggtgcaggc ggccggccac
     6301 aacccctacc gttttgcgcc cgacgcgccg gaactggcct cccttcgaaa cgccgaacac
     6361 accggcatta accacaagtc cgttcccacg atttacccac cgctcgccca ggcggtgttt
     6421 cgcctcgccg tcgccctttc gcccacgttg gcg
//
LOCUS       DAOQLS010000018         6282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5296_length_6282_cov_4.314437, whole genome shotgun sequence.
ACCESSION   DAOQLS010000018 DAOQLS010000000
VERSION     DAOQLS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5296_length_6282_cov_4.314437"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PK362_00625"
     CDS             complement(<1..1018)
                     /locus_tag="PK362_00625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC73377.1"
                     /translation="MSFLDINLHPDLVRAVEALGWTDPTPIQRQAVPLILEGRDVLGA
                     AQTGSGKTGAFALPILDHLLRKPSPGLRLLVLVPTRELASQVEQTFRDVGRFTSFKVA
                     TVIGGVGYDQQRRAVSEGAEIMVATPGRLLDHLENRAFRLTTVDHLVMDEADRMLDMG
                     FLPDIRAILHYVPKERQTLLFSATLVPDVERVAAFTLKDPVRVEVARPATLAEGISQV
                     LYPVVQEQKIDLLLTLLKNTEMRSVLAFTRTKDRADRLAASLDKAGYRVEVLHSNRTQ
                     RERTDAMENFREGKSQILVATDIAARGIDVKGISHVINFDVPQHPEDYVHRVGRTARA
                     YGVGD"
     gene            complement(1065..2369)
                     /gene="tssI"
                     /locus_tag="PK362_00630"
     CDS             complement(1065..2369)
                     /gene="tssI"
                     /locus_tag="PK362_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HNC73378.1"
                     /translation="MVLPALEWENAPSEKFLVKSISRIETVGRLSDTRVTGEPRGDFR
                     PERFLGQEVSFLLSNPPEAPRRLHGTLVSVTRESSAEDAPMVLLEVAPWLQELTKSAN
                     LAVFQDRSVPEILESVFRRWPRSLFVFDLHSSHPRRTLVCQYRETDFNFASRLMEEEG
                     LFYYFRDVPEGQRMIITDRNSPDPVGTVRFKERSSGDAGLDRWVEKFETTPSGYTLMD
                     YGVEAATPIVVSAATEPTGGPNVFRDALDTRFTLEEGPRFARLRLEENRAARAVYSAS
                     GNEPSLAAGDRFRLKGHPDPRQNADYWVTETNAQWYLVEPPPEETGDEEVPPLDALPP
                     LGMKFQFSLSAIPADTPFRPRRHTPKPLQQGVDMAEVVAVEGARVQILFFWNTPALGG
                     TPHPLWVPSLLATPPRPGDRVIVEFLAGDPDRPLVTAPAPLP"
     assembly_gap    2567..2666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2703..3314)
                     /locus_tag="PK362_00635"
     CDS             complement(2703..3314)
                     /locus_tag="PK362_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73379.1"
                     /translation="MAQRLFFRSSRPAVAARPVSAPRRIARGLVLAAAAVGAAYFLLP
                     FRTSGADALRRHFAARRADFVRLRDMIAAEPSLASIGVDNVGEYWLFDGSWTASDRRF
                     VAYSRREMLEAAGLPAERYDRYLALLSRVGAYRVARQNVLGAPLRGTVFLFPDPQGRG
                     PVTNIVFSHRPPEPLLAWDAAERNARPTYAALDDGWYVEFRRR"
     gene            complement(3318..3629)
                     /locus_tag="PK362_00640"
     CDS             complement(3318..3629)
                     /locus_tag="PK362_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73380.1"
                     /translation="MNRSLERQIVFFTVVGKVSFGDLDGCATVEVLRGRGGRQVAAGP
                     TRSPGRRETAVWVDDGGRRRLFRTAVHAMAAVERERPGESLHWCRKVRLQPSLGRMPA
                     R"
     gene            complement(3745..5055)
                     /locus_tag="PK362_00645"
     CDS             complement(3745..5055)
                     /locus_tag="PK362_00645"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNC73381.1"
                     /translation="MNRRLSPLGLGILLLATASPAGDLAGRDRRYLKGVYRDTWACLA
                     HFVGEGSGLPYDSARRLPSTSLTNVGLYLAATAVAARTGLLTGDEARARLDRALTSLV
                     KIERWGYGFPVTWVNVDTLEPTENQFSTVDHLSHLVGGLLVVKGLFPEYAPTVDDVLQ
                     PMDWGGLYDPARRWLKGGWRRDRKDFDVRQKGWDWYYSYVGADTRFGYVYGIGRGQIP
                     PESWSALNHDREKKHGLSYFVPGWQGGGLFMQMVSGLFVDEKPSALGRSAADFAWAQT
                     LHARSLGSPVWGWSACESPDGKSYLGWGHIDDAVVTPHAAALAAVYYPRQATENLRRL
                     EKMGVRAPWDGGADGRRPFGFRDSLNWKTGEVSGNYLSLDQGMLFLALANTLHDGIVW
                     KAVAQDPTVRRGVEAISDFAGDPASPAEYRRRDAEPFLLPPAGR"
     gene            complement(5196..>6282)
                     /gene="serS"
                     /locus_tag="PK362_00650"
     CDS             complement(5196..>6282)
                     /gene="serS"
                     /locus_tag="PK362_00650"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNC73382.1"
                     /translation="DATALLKEMEELKTRLKGLDDRTAAAQARVDEKVLGLPNLPHPS
                     VPVGADAGQNREIRRWGEPKKFPFKPRDHQDIGEKLGVLDMARAAKLSGARFALLSGA
                     GAKMERALIHFMLDLHTGVHGYTEIFPPFLVTRQTMTGTGQLPKFADELYATKDDDLF
                     LIPTAEVPLTNLYRDEPLDEATLPRALCAYTACFRREAGSYGKDTRGLIRNHQFNKIE
                     LVRFVRPEDSLAELEKLTGHAEEVLKRLGLPYRVMELCTGDLGFSSAKTYDLEVWLPA
                     ENGGQGAYREISSCSTFTDFQARRIGLRYKKSDGSRALMHTLNGSGVAVGRTLAAVLE
                     NYQDADGSVAVPEALRTLMGIDRLSPP"
BASE COUNT         1178 a         1902 c         2085 g         1017 t
ORIGIN      
        1 cgtctcccac gccgtaggcc cgggcggtcc gacccacccg gtggacgtag tcctcggggt
       61 gctggggcac gtcgaaattg atgacgtggg aaatgccctt cacgtcgatg ccccgggccg
      121 cgatatcggt ggcgacgagg atctgggatt tcccttcccg gaaattttcc atggcgtcgg
      181 tgcgttcccg ctgggtgcgg ttcgaatgaa gcacctcgac ccggtagccc gccttgtcca
      241 gggaagccgc cagacggtcc gcccggtctt ttgtccgcgt aaaggcgagc accgaacgca
      301 tttcggtgtt tttgaggagc gtcaacaaca aatcgatttt ttgttcctgc accaccggat
      361 acaggacctg gctgatgcct tcggccagag tggcgggacg ggcgacctcg acccggacgg
      421 gatctttcag ggtgaaggcc gcgacccgtt ccacgtcggg aacaagcgtg gcggagaaca
      481 ggagcgtctg gcgttcctta ggcacgtaat ggagaatggc ccgaatgtcg ggcaaaaagc
      541 ccatgtccaa catgcgatcg gcttcgtcca tgaccagatg atccaccgtc gtcaatcgga
      601 acgcccggtt ctccaaatgg tccagcaacc ggcccggcgt ggcgaccatg atctcggcgc
      661 cctcggaaac ggcccggcgc tgttggtcgt accccacgcc gccgatgacg gtcgcgactt
      721 tgaacgacgt gaaacgcccc acgtcccgga aggtttgctc gacctgggaa gccagttcgc
      781 gggtggggac caacaccagc aagcgaagcc ccggggaggg tttgcgcaag aggtggtcca
      841 aaatgggcag ggcgaaggcc ccggttttcc cgctgccggt ctgggcggcg cccagcacgt
      901 cgcgtccctc caaaatgagg ggaacggcct gtcgttggat cggcgtcgga tccgtccaac
      961 cgagcgcctc cacggcgcgg accagatcgg ggtgaaggtt gatgtcgaga aaggacatgg
     1021 aggaagatta tttcattttc cgaggaattc ggggggccaa aaacttaagg gaggggggcc
     1081 ggggcggtga ccaggggacg gtcgggatcg ccggccagga attcaacgat cacgcgatcc
     1141 ccggggcgcg ggggggtcgc caacagactc ggaacccaca ggggatgggg cgttcccccc
     1201 agggcggggg tgttccaaaa aaagagaatt tggacccggg cgccctcgac cgccaccacc
     1261 tcggccatgt cgaccccctg ttgaagaggt ttgggcgtat gacgccgggg gcgaaagggc
     1321 gtgtcggccg ggatcgcgga caaggaaaat tgaaatttca tgcccaaggg gggaagcgcg
     1381 tcaagcgggg ggacctcctc gtcgcccgtt tcttcgggtg gcggttcgac gaggtaccac
     1441 tgagcgttgg tctccgtcac ccagtagtcc gcgttttgcc gagggtcggg gtggcccttg
     1501 agtcgaaagc ggtcccccgc ggccagggaa ggctcgttgc ccgaggccga ataaaccgcc
     1561 cgggcggcgc ggttttcctc caggcgaagc cgggcgaaac gcggcccctc ctcgagggtg
     1621 aatcgggtgt ccagggcgtc ccggaaaaca ttcggaccgc cggtcggctc cgtcgccgcg
     1681 ctgaccacga tcggcgtggc cgcctccacc ccgtaatcca tgagggtgta accgctcggc
     1741 gtcgtttcaa atttttccac ccaacgatcc agacccgcgt cgccggagga tcgttctttg
     1801 aaccggaccg ttcccaccgg gtccggggaa ttccggtccg tgatgatcat ccgttgccct
     1861 tccgggacgt cccggaagta ataaaacaac ccttcctctt ccatcaatcg cgaggcgaaa
     1921 ttaaagtcgg tttcccggta ctgacaaacc aacgtccgcc ggggatggga cgaatggagg
     1981 tcgaaaacga agagggagcg gggccatcga cggaacacgc tctcgagaat ttccggcacc
     2041 gagcggtcct gaaaaaccgc caggttggcg gatttggtca gttcctgaag ccacggcgcc
     2101 acttcgagga ggaccatcgg cgcgtcctcc gcgctcgatt cccgcgtcac cgaaacaagc
     2161 gtgccgtgaa gacgccgggg ggcttcgggg ggattggaga gaagaaaact gacttcctgc
     2221 cccaggaacc gctcggggcg gaaatccccc cggggttcgc ccgtcacccg ggtatccgaa
     2281 aggcgaccga cggtctcgat ccgggaaatc gatttaacga gaaacttttc cgatggagcg
     2341 ttttcccatt ccaaggcggg aaggaccacg cgcggcggtg gtcaaggcca gggtcaaaaa
     2401 ggtcgaaaca acgagggacg ttttgggcac gggtttaagg ccgcgtttcc ttggcgtcgc
     2461 tgtgtttcgg attggcgaga aggctttcac tcggggcgca ttccgggaac gagaggcatt
     2521 gagttggaaa tggagtattc aaaaccgcgc cgccccttcc cccgggnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnaacc gcgccgcccc ttcctacaaa cagggacggc
     2701 gcctagcggc gccgaaactc gacgtaccaa ccgtcgtcga gggcggcgta ggtcgggcgg
     2761 gcgttccgtt cggcggcgtc ccaggccaac aggggttcgg gcgggcgatg ggaaaaaacg
     2821 atgttcgtca cggggccccg gccctggggg tcgggaaaga ggaaaacggt cccgcgcagg
     2881 ggggctccca gaacgttttg ccgggccacc cgataggcgc ccacgcgact caaaagggcg
     2941 aggtagcggt cgtatcgttc ggcgggaagc cccgcggctt cgagcatttc ccggcggctg
     3001 taggcgacaa accgccggtc cgaggcggtc caggaaccgt caaagagcca atattcgccc
     3061 acgttgtcca cgccgatgga cgccagggac ggctcggcgg cgatcatgtc cctgaggcgc
     3121 acgaaatcgg cccgccgggc ggcgaaatgc cgtcgaaggg cgtccgcgcc ggaggttcga
     3181 aaaggcaaaa ggaaataggc ggcgccgacc gcggccgcgg ccaaaaccaa tccccgggcg
     3241 atccggcgcg gggcggacac gggccgggcc gcgacggccg gacgggagga gcggaaaaaa
     3301 aggcgttggg ccatggctta gcgggcgggc atccggccca gggacggttg aaggcgcact
     3361 ttgcggcacc agtggagcga ttcgccgggg cgctcgcgct cgacggccgc catggcgtgg
     3421 acggccgtgc ggaacaatcg gcgtcggccc ccgtcgtcga cccacaccgc cgtttcgcga
     3481 cgacccggag agcgggtcgg accggcggcc acctgcctgc cgccccggcc gcgaagcacc
     3541 tccacggtgg cgcacccgtc cagatcgccg aaggagactt tccccacgac ggtaaaaaag
     3601 acgatttgac gctccaagga acggttcatg gcattagacg acaacgcccc ggcccgcggc
     3661 ggggcggtcg gggcgattat tcaacggcca acctatggtg tggccttcca ttgggacaca
     3721 atatatatag cccccaggcc gatgtcaacg tcccgcgggg ggaagcagaa aaggttcggc
     3781 gtcccgtcgg cggtattcgg cgggggaggc cggatccccg gcaaagtcgg aaatggcctc
     3841 cacgccccgc cggaccgtcg gatcctgggc gacggctttc cagacgatcc cgtcgtgaag
     3901 ggtgttggcc agagccaaaa acagcatccc ttgatccagg ctcaaatagt tgccggacac
     3961 ctccccggtt ttccaattaa gactgtcccg aaacccgaag ggccgacggc cgtcggcccc
     4021 gccgtcccag ggcgcccgga cgcccatttt ttccagtcga cgcaaattct ccgtcgcctg
     4081 gcgagggtaa taaacagcgg cgagggcggc ggcgtggggc gtcaccacgg cgtcgtcgat
     4141 gtgcccccat cccaaataag atttaccgtc gggactctcg caggcggacc atccccaaac
     4201 cggggaaccc aacgaacggg cgtggagggt ttgggcccag gcgaaatcgg cggccgaccg
     4261 gcccaaggcc gaaggttttt cgtccacgaa gagcccggaa accatctgca tgaacagccc
     4321 gcccccctgc caaccgggaa cgaaatagga aaggccgtgc tttttttccc gatcgtggtt
     4381 taaggcggac cagctttcgg ggggaatctg accccggccg atgccgtaga cataaccgaa
     4441 gcgggtgtcc gcccccacgt agctgtagta ccaatcccac cccttttggc ggacatcgaa
     4501 atccttgcga tcccggcgcc acccgccttt cagccagcgg cgggcggggt cgtaaagccc
     4561 gccccagtcc atgggttgga ggacgtcgtc caccgtgggg gcgtattcgg gaaacaaccc
     4621 tttgaccacc aataaaccgc ccaccaaatg ggacaggtgg tccacggtgg aaaattggtt
     4681 ttccgtcggc tccagggtgt cgacgttgac ccaagtgacg gggaaaccat atccccagcg
     4741 ttcgattttc accaggctcg tcaaagcgcg gtccaaacgc gcccgggcct cgtcgccggt
     4801 caggagaccg gtgcgggcgg cgacggcggt ggccgccagg taaaggccga cgttggtcag
     4861 ggaggtggag ggaagacgtc gggcggaatc gtagggaagg cccgatccct cgccgacaaa
     4921 atgggcgagg caggcccagg tgtcccggta aacgcctttc aggtaacgac ggtcccggcc
     4981 ggccaggtcg ccggcggggg aggcggttgc caacaggagg atcccgaggc ccaggggcga
     5041 gaggcgccgg ttcaccgaaa gggagggcgt cggcccccgg cgatcacaac ccgcgccgcg
     5101 ccgaaagcgt cgaaggaagg cccccgcggg gcgacccgta tataatccgc gattttttta
     5161 cccatgggaa ctcccggtgg aattgattgg gggaattagg ggggggacag ccggtcgatg
     5221 cccatcagcg tccggagggc ctcgggcacc gcgaccgacc cgtcggcgtc ctggtaattt
     5281 tccagcacgg ccgccagggt ccggcccacg gccacgccgg acccgttgag cgtgtgcatc
     5341 agcgcccggg agccgtcgct ctttttgtag cgcaggccga tgcgccgggc ctgaaaatcc
     5401 gtaaaggtgg agcaggacga aatttcccgg taggccccct gcccgccgtt ttcggccggg
     5461 agccagactt ccaaatcgta ggttttggcc gaggaaaacc cgaggtcgcc ggtgcagagc
     5521 tccatcaccc ggtagggcaa cccgagacgt ttcagcactt cctccgcgtg accggtcaat
     5581 ttctccagct ccgccaggga atcctcgggt cggacgaagc ggacgagctc gattttattg
     5641 aattgatgat tgcggataag cccccgggtg tctttcccgt aggagccggc ttcccggcga
     5701 aagcaggccg tgtaggcgca cagggcccgg ggcagggtgg cctcgtccaa cggctcgtcc
     5761 cggtagaggt tggtcagggg cacttccgcg gtggggatga ggaacaaatc gtcgtctttg
     5821 gtggcgtaaa gttcgtcggc gaatttgggc agctgaccgg tgccggtcat ggtctggcgg
     5881 gtgacgagga acggggggaa aatttccgtg tacccgtgaa ccccggtgtg gagatccagc
     5941 atgaaatgga tcagcgcccg ctccatcttc gcgccggcgc ccgagaggag ggcgaagcgg
     6001 gccccggaga gcttcgcggc ccgggccatg tccagcaccc caagtttttc gccgatgtct
     6061 tgatggtccc ggggtttaaa aggaaatttt ttgggttcgc cccaacggcg gatttcacgg
     6121 ttttgtccgg cgtccgcgcc cacgggtacc gacggatgcg gaaggttcgg caggcccagc
     6181 accttttcat ccacccgggc ctgggccgcg gcggttctgt cgtccaagcc cttgagacgg
     6241 gttttaagtt cctccatttc cttcaggagg gccgtcgcgt cc
//
LOCUS       DAOQLS010000019         6275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5313_length_6275_cov_4.318810, whole genome shotgun sequence.
ACCESSION   DAOQLS010000019 DAOQLS010000000
VERSION     DAOQLS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5313_length_6275_cov_4.318810"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK362_00655"
     CDS             complement(<1..516)
                     /locus_tag="PK362_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC73383.1"
                     /translation="MPNAIFVDKHRCTGCTSCSRVCPVSCIDMVERPKEPGVTWRKLA
                     LIDEAKCIFCNACVQACDKLFEKSKNKDVFHAITMVKEKVEGKTTVDVSLYKNVWVFA
                     EMRHGKLMPTAYELLGVARGLAATLGEEVAAVLMGKDVAQHAQDLIDHGADRVYVLDH
                     PSLENFVDEIYT"
     gene            complement(519..1322)
                     /locus_tag="PK362_00660"
     CDS             complement(519..1322)
                     /locus_tag="PK362_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNC73384.1"
                     /translation="MHIVVCIKQTPATSNIQIDPATGTLKREGMAAAINPFDAYAIEE
                     AVRIKERLPGTTVSVITMGPPQAEDSLREAIACGADQAFHLTSRAFAGADTWATSYTI
                     QMGIRKIMKEKGPVHLVICGKQTNDGDTGHVGPGIAAWLDWPNIAYVKKVEAVDDKKI
                     VVHRMMEDGVDVLEMDLPAVLAVVKEINEPRVASLKGKMNAKKAVIPKWTEVDIEADK
                     KCIGLGGSPTIVSKSFNPPPRKGGVKIEGATAQEKAKNLVDKLQEMKLI"
     gene            complement(1432..2661)
                     /locus_tag="PK362_00665"
     CDS             complement(1432..2661)
                     /locus_tag="PK362_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC73385.1"
                     /translation="MNYFLTEEQQAIVQVAREIAQKKIKPVREKHDAEDTFAWEIVEE
                     FRKADLCGVYFHTNYGGLGGGVFELALVVEELSKVCGGIALSLAATALGTFPIMLFGT
                     PEQKKRWLPDLASGKRLGAFTITEPEAGSDATATRCTAKKDGNDYILNGVKNFATNGE
                     VAEIYSTFASTNPSRGARGITCFVIEKGTKGFSFGKKEHKMGIRSSATYELVFDNCRI
                     PAANMLGKEGQGLFVAQATLDISRPGVASQALGIGTGALEEALNYAKVRKQFGQTVLS
                     FQSSQHMVADMATKLEAARALLYSVVRAMDVDFKAAVEASEQSGKTVYEEMKRVASGR
                     WTKYSAMCKLFCSDTAFEVADNAIQLCGGIGYMRDFPVEKYLRDAKITQIYEGTNQIQ
                     RNEIGMMITKELASKGE"
     gene            complement(2748..3530)
                     /gene="rnc"
                     /locus_tag="PK362_00670"
     CDS             complement(2748..3530)
                     /gene="rnc"
                     /locus_tag="PK362_00670"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNC73386.1"
                     /translation="MPREDSALLQKALRHKFKNAKLLEEALTHKSHAIERGSKSFNER
                     LEFLGDSVLAAVVAHYLFKRYADEDEGKLSKLKSQLVARPSLVVWSREIGLGKYLWMS
                     DGEEATGGRERDSLLANAFEALLGAIFLDAGFSVAQRFVVRLLSKKKRIVETDYKSKL
                     QEIIQKRYKIPPSYLLLEEKGPDHNKTFVMQVNVRRRLLGQGEGHSKKEAEQAAAYQA
                     LKKIRQHRLAPKIDPHHMAEDHALSEPSPRRAPRRTEEKEKS"
     gene            complement(3540..4790)
                     /gene="fabF"
                     /locus_tag="PK362_00675"
     CDS             complement(3540..4790)
                     /gene="fabF"
                     /locus_tag="PK362_00675"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNC73387.1"
                     /translation="MDNKKRVVITGVGIVSPIGIGKDAYWQSLKSGRPGFGRISFFDP
                     STYPCHIDAEVRDFQPEQFIDKKKIRRMDRFTQFACAAAKLAVEDSKIDFTKENVERC
                     GVIVGSGIGGLPTIESEHSVLLEKGMRRVSPFLIPMLITNIAPGEIAIEYGLVGPNYS
                     VASACATSNHAIGEALRHLRYGDADVILAGGTEAAITPLGVAGFCQARALTTDFNDRP
                     EKASRPFDKGRSGFVMGEGAGVVVLETLEHALARGAKIYGELAGYGATDDAYHITAPS
                     PEAKAASRAMQLALADGGLRPEDVDYVNAHGTSTELNDKTETFAIKKVFGDHAKKLAV
                     SSTKSMTGHLLGAAGAAELIATLLSMENNTLHPTINQETPDPECDLDYVPNVARPAQI
                     NVALSNSLGFGGHNAVLVVKRFKP"
     gene            complement(4881..5120)
                     /gene="acpP"
                     /locus_tag="PK362_00680"
     CDS             complement(4881..5120)
                     /gene="acpP"
                     /locus_tag="PK362_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC73388.1"
                     /translation="MADDIETKVRDIIVEQLGVDAAQVKPEASFVNDLGADSLDTVEL
                     VMALEEAFDMEIPDEEAEKIQTVTQAVNYIKSHKK"
     gene            complement(5252..5995)
                     /gene="fabG"
                     /locus_tag="PK362_00685"
     CDS             complement(5252..5995)
                     /gene="fabG"
                     /locus_tag="PK362_00685"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNC73389.1"
                     /translation="MRLKDQVTLITGGAQGIGRAIAETFAKEGAKIALCDVNEAAAQQ
                     TAAEIKQKYNVDTHAAKVNVTVFDECEKWVAAVAEKMGRVDVLINNAGITKDNLVMRM
                     SDQEWDAVIAVNLKGVFNCTKAVNRPMFKQRGGRIINIASIVGLMGNAGQVNYSATKG
                     GVIAMTKTCAREFASRNVLVNAIAPGFIRTAMTDKLTDEQKQKLSSIIPLERLGEAQD
                     VANAALFLASPESSYITGQVISVNGGMYM"
     gene            complement(6034..>6275)
                     /locus_tag="PK362_00690"
     CDS             complement(6034..>6275)
                     /locus_tag="PK362_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HNC73390.1"
                     /translation="RETRSPEEIRAKLVRQIDHAVLWEDTLRGLFAAGVENFIEVGPG
                     RVLSGLLRRTDKAKKFSNIEDKKSADALLAAAAPR"
BASE COUNT         1020 a         1840 c         1981 g         1434 t
ORIGIN      
        1 ggtgtagatt tcgtccacga agttttccag ggacggatga tccagcacgt acacgcgatc
       61 ggcgccgtgg tcgatcaaat cctgggcgtg ttgggcgacg tctttgccca tgagcaccgc
      121 ggccacttct tccccgagcg tcgccgccaa cccccgggcg acccccaaca gctcgtaggc
      181 cgtgggcatc aatttcccgt gacgcatctc cgcgaacacc cagacgtttt tgtacagcga
      241 cacgtcgacg gtggttttgc cctccacttt ttccttgacc atggtgatgg cgtggaagac
      301 gtctttgttt ttggatttct cgaacagctt atcgcaggcc tgaacgcagg cgttgcagaa
      361 aatgcatttg gcttcgtcga tcaaggcgag tttgcgccag gtgacgccgg gctccttggg
      421 ccgctccacc atgtcgatgc aggagacggg gcaaacccgg gagcaggagg tgcagccggt
      481 gcaacggtgt ttgtcgacga agatcgcgtt gggcatggtt agatcagctt catttcctgg
      541 agcttgtcca cgagattttt ggctttttcc tgggcggtgg cgccctcaat tttgaccccg
      601 cctttgcgcg gcggcgggtt gaaggatttg gaaacgatcg tgggcgatcc gcccaacccg
      661 atgcatttct tgtcggcctc gatgtccact tccgtccatt tggggatgac cgcttttttg
      721 gcgttcatct tgcctttgag ggaagccacc cggggctcgt tgatttcctt caccacggcc
      781 agcacggcgg gcaaatccat ttccaacacg tcgacgccgt cctccatcat tcgatggacg
      841 acgatctttt tatcgtcaac ggcttccact tttttgacgt aggcgatgtt cggccagtcc
      901 agccaggcgg cgatgccggg gcccacgtga cccgtgtcgc cgtcgttggt ctgcttgccg
      961 cagatcacga ggtgcacggg acctttttcc ttcatgattt tccgaatgcc catttgaatg
     1021 gtgtaggaag tcgcccaggt gtcggcgccg gcgaaggcgc ggctggtcaa atggaaggcc
     1081 tggtcggccc cgcaggcgat ggcttcgcgg agggaatcct cggcctgggg cggtcccatg
     1141 gtgatgacgg agaccgtggt gcccggcagc ctttccttga tgcggacggc ctcttcgatg
     1201 gcgtaggcgt cgaaaggatt gatggccgcc gccatgcctt cgcgcttgag cgttccggtg
     1261 gcggggtcga tttgaatgtt cgatgtcgcg ggcgtctgct tgatgcagac gacgatgtgc
     1321 aatcccatgg cggtcgtctc tttacttagg ttatcggcag gttcgtgatg tttttcacga
     1381 agcgatgcac cattctaact ggaaattttc gtcttttcaa ggaggaaccg tttattcccc
     1441 cttggacgcc aactccttgg tgatcatcat cccgatctcg ttgcgttgga tctggttggt
     1501 gccttcgtaa atttgggtga tcttcgcgtc ccggaggtat ttctccaccg ggaagtcccg
     1561 catgtagccg atgccgccgc acaactggat ggcgttatcg gccacttcaa aggccgtgtc
     1621 cgagcagaac agcttgcaca tggccgagta cttggtccag cggccggagg ccacacgctt
     1681 catttcctca tagaccgttt ttcccgattg ctcggaggct tccaccgccg ccttgaagtc
     1741 gacgtccatg gcccgcacga cgctgtaaag cagcgcccgg gcggcttcga gcttggtggc
     1801 catgtcggcc accatgtgct gggaagattg gaaggagagg accgtttggc cgaattgctt
     1861 gcgcaccttg gcgtagttga gcgcttcttc cagggcgccg gtgccgatgc ccagggcctg
     1921 cgaagcgacg cccggccggg agatatcgag ggtcgcctgg gccacgaaca ggccctggcc
     1981 ttccttgccg agcatgttgg cggccgggat gcggcagttg tcaaacacca attcgtaggt
     2041 ggcgctggag cggatgccca tcttgtgttc ttttttaccg aaggagaatc ccttggtccc
     2101 cttttcgatc acgaagcagg tgatcccgcg ggcgccccgg gaggggttgg tcgaggcgaa
     2161 ggtcgaatag atttcggcca cttcgccgtt ggtggcgaaa ttcttcacgc cgttcaaaat
     2221 atagtcgttg ccgtctttct tcgccgtgca gcgtgtggcc gtggcgtcgg agccggcttc
     2281 gggttccgtg atggtgaagg cgcccagacg tttgccggag gccaggtcgg gcagccagcg
     2341 ctttttttgt tccggcgtgc cgaaaagcat gatggggaag gtgccgaggg ccgtggccgc
     2401 caggctgagg gcgatgccgc cgcacacttt ggacaattcc tcgacgacga gggccaattc
     2461 gaagaccccc ccgcccagac cgccgtaatt cgtgtggaaa tagacgccgc acaaatcggc
     2521 cttgcggaac tcctcgacga tttcccaggc gaaggtgtcc tccgcgtcgt gcttctcccg
     2581 gacgggtttt attttctttt gggcgatttc gcgcgcgact tggacgatgg cctgttgctc
     2641 ttcggtcaag aaatagttca tgacgtctcc ttcgggggtg aactcaaatt cgatgggtcc
     2701 ggccggtcaa ctatacaaaa atcgccgggg ggcaaacgtg acgcaactca ggatttttcc
     2761 ttttcctccg tccgccgggg ggcccgtcgg gggctcggct ccgacaacgc gtgatcctcg
     2821 gccatgtggt ggggatcgat tttcggggcc aatcggtgct gacggatctt tttcaaggcc
     2881 tggtacgccg cggcctgctc cgcttccttc ttggaatgcc cctcgccctg gcccagaagg
     2941 cgtcggcgga cgttcacctg catgacgaag gttttgttgt ggtcggggcc tttctcctcc
     3001 aggagcaggt aggagggcgg aatcttgtag cgcttctgga tgatttcctg aagtttgctt
     3061 ttgtaatcgg tttcgacgat tcgcttcttt ttggaaagga gccggaccac gaaccgttgg
     3121 gccaccgaaa acccggcatc gaggaaaatc gcccccagga gggcctcgaa ggcgttggcc
     3181 agcaggctgt cccgctcgcg gccgccggtg gcttcctcgc cgtcgctcat ccacaaatat
     3241 ttgcccaggc cgatttcccg ggaccagacg accagggagg gccgggccac cagctgggat
     3301 ttcagtttgg acagtttgcc ctcgtcttcg tcggcgtagc gtttaaacag gtaatgggcg
     3361 acgaccgccg ccaggacgct gtcgcccaaa aactcgaggc gctcgttgaa ggacttgctt
     3421 ccccgctcga tggcgtggga tttgtgggtg agggcttctt ccaggagctt cgcgtttttg
     3481 aatttgtgcc gaagcgcttt ttggagaagg gcggagtcct cgcgcggcat gacgtcccgt
     3541 tagggtttga accgtttcac caccaacacg gcgttgtgcc cgccgaagcc cagggagttg
     3601 gagagcgcca cgttgatttg ggcggggcgg gcgacgttcg gcacgtagtc caaatcgcat
     3661 tccgggtcgg gcgtttcctg gttgatggtc ggatgcaggg tgttgttttc catgctgagg
     3721 agcgtggcga tcaattccgc cgccccggcc gcgcccagca ggtggccggt catggatttg
     3781 gtggaggaaa cggcgagctt cttcgcgtgg tcgccgaaca cctttttgat ggcgaaggtt
     3841 tcggttttat cgttgagttc ggttgaggtg ccgtgggcgt tgacgtaatc gacgtcctcg
     3901 ggccgcaagc cgccgtcggc cagggccagc tgcatggcgc ggctggcggc cttggcttcc
     3961 ggcgagggcg cggtgatgtg gtacgcgtcg tccgtggcgc cgtagcccgc caattccccg
     4021 taaattttcg cgccgcgcgc caaggcgtgt tccagggttt ccaggaccac gacgccggcg
     4081 ccttcgccca tcacgaaccc cgaccggccc ttgtcaaagg gacggctggc cttttcgggg
     4141 cggtcgttga agtccgtggt cagggcccgg gcctggcaga agccggccac gcccaggggg
     4201 gtgatggcgg cctcggtgcc gccggccaaa atcacgtcgg cgtcgccgta acgcaggtgc
     4261 cgcagggctt ccccgatggc gtggttcgag gtggcgcagg ccgaggccac ggaatagttg
     4321 ggacccacga ggccgtattc aatggcgatc tcgccggggg cgatgttcgt gatgagcatc
     4381 gggatcagga agggcgacac ccggcgcatg cctttttcca gcaacacgga atgttcggat
     4441 tcgatggtgg gcaatccgcc gatgccggag cccacgatga ccccgcaccg ttccacgttt
     4501 tccttcgtga aatcgatttt ggaatcctcc acggccaatt tggccgccgc gcaggcaaat
     4561 tgggtgaacc gatccatgcg ccggattttc tttttatcga taaattgctc cggctgaaaa
     4621 tcccggactt ccgcgtcgat gtggcagggg taggtggacg ggtcaaaaaa actgatccgc
     4681 ccgaacccgg gacgcccgga ttttagactc tgccagtagg cgtccttgcc gatgccgatg
     4741 gggctgacga tccccactcc ggtgatgacc acgcgctttt tattgtccat ggaatcctct
     4801 tcgcggacgc cgaaaccacg ggcgtccaaa atcgtgaccc cgaagggagg ctgatcgtcc
     4861 cttcggggtc cggggaaaaa ctatttcttg tgcgacttga tgtagttgac ggcctgggtg
     4921 acggtttgga tcttttcggc ttcttcgtcg ggaatttcca tgtcgaaggc ctcttccaac
     4981 gccatcacga gctccaccgt gtccagggag tccgcgccga ggtcgttcac gaacgacgcc
     5041 tccggcttca cctgcgccgc gtccacgccc aattgctcca cgatgatgtc ccgcactttt
     5101 gtctcgatgt cgtctgccac gtgttgcccc cttcattgag gttgttggcg tctgaggccc
     5161 aacgtgcgtg gggggacttc cccgcgcgcg ttgagcttcc tccaccgcgc ttctgaaaaa
     5221 cgacggcccc gacgcttccg gggcccggac gctacatgta catcccgccg ttcacggaaa
     5281 tcacctgccc cgtgatgtag gagctctcgg gcgaggccaa aaacagggcc gcgttggcca
     5341 cgtcctgggc ttcgcccagg cgttccaagg ggatgatgct ggaaagtttc tgtttctgct
     5401 cgtcggtcaa tttgtcggtc atggccgtcc ggatgaaccc cggggcgatc gcgttgacca
     5461 ggacgttccg gctggcgaat tcccgggcgc aggttttggt catggcgatg acgccgccct
     5521 tggtggccga atagttgacc tggcccgcgt tgcccatgag gcccacaatg gaggcgatgt
     5581 tgatgatgcg gcccccccgc tgcttgaaca tcgggcggtt gacggccttg gtgcagttga
     5641 acacgccctt gaggttcacg gcgatgaccg cgtcccattc ctgatccgac atccgcatca
     5701 ccaggttgtc cttggtgatg ccggcgttgt tgatcaaaac gtccacccgg cccatttttt
     5761 cggccacggc ggcgacccac ttttcgcatt cgtcgaacac cgtcacgttc actttggcgg
     5821 cgtgggtgtc cacgttgtat ttttgcttga tttcggcggc ggtctgttgg gccgcggctt
     5881 cgttcacgtc gcaaagggcg attttggcgc cttccttggc gaaggtttcg gcgatggccc
     5941 gcccgatgcc ctgggccccg ccggtgatga gagtcacttg atctttgagt cgcatggtgt
     6001 gtcctttcgc gagaggaaaa cgaacgccgc gccttagcgc ggggcggcgg ccgcgagcaa
     6061 cgcgtccgcc gattttttgt cctctatatt agaaaatttc ttggccttgt cggtgcgccg
     6121 caggagcccc gacagcaccc gccccgggcc gacctcgatg aaattctcga cgcccgcggc
     6181 gaagagtccg cgcagggtgt cttcccagag cacggcgtgg tcgatttggc ggacgagctt
     6241 cgcccgaatc tcctcggggg aacgggtttc ccggg
//
LOCUS       DAOQLS010000020         6271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5320_length_6271_cov_4.910071, whole genome shotgun sequence.
ACCESSION   DAOQLS010000020 DAOQLS010000000
VERSION     DAOQLS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5320_length_6271_cov_4.910071"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PK362_00695"
     CDS             complement(<1..555)
                     /locus_tag="PK362_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73391.1"
                     /translation="MRRRRSAFFRSAPRWFLGLLPAASLFAGTPGVAGEGPKRVIVRY
                     RSSPAGKSPTVVGAPALNGLSAAFGVRSLGPVFRAPPAASAKQGAPARSTLQQSLRRD
                     LESVQVLIYEGPDDPEVVARAYAADPAVDWAEPDARFEAFRVPNDPDYSDLWALPKIN
                     APEAWDASTGGGVVVAVVDSGVDGA"
     gene            complement(560..2704)
                     /locus_tag="PK362_00700"
     CDS             complement(560..2704)
                     /locus_tag="PK362_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC73392.1"
                     /translation="MNKLSTEKSPYLLQHATNPVHWLPWGDEAFETAFREDKPILVSI
                     GYATCHWCHVMERESFENPAIAEVMNRHLVCVKVDREERPDVDRLYMTAVQAMTGQGG
                     WPLNVFLTPTGKIFLGGTYFPPADRGGRPGWPRLVQRIGEAWKNPPERESLERQGEEL
                     VRAVAGFLSGSRTVAEADAEEAVSKAFDAFRESFDPDHGGFSGAPKFPLPVNQHFLLR
                     FAARAEALADPRGKTARDMAVRTLAALAAGGICDQLGGGFHRYSTDDRWHLPHFEKML
                     YDNAQLAVNFLEAHQLTGDPDLARTARSTLDYLVRDLGNAQGGFFSAEDADSRPEDSP
                     PGADKKEGAFYVWTWSELSKILGEEAEVFARAHGVRPEGNVDDGTGEFAGENVLCRAR
                     SEADLAAEGGWTIEDVSRGLAGSRSTLFDRRRSRPRPFLDDKILTGWNGLALSALAKG
                     HAILGEDRYREAAVRCAEFLKSRLWDAAGGRLYRRWRDGERAVAGQADDHAFLIQGLV
                     DLYEATFDSSWLRWAETLAEVFLQTFVDRESGVVHATPPDQDPRLPVRARDEGDNVEP
                     GASSVAVAVLQRLGRWRARADFTAMAGKISDALRGRATEAPRAFPGFLSAVVVDLCPK
                     SELVIAGRPGNEATRRLLAEARRVFRPDLLVVLADQAEGQAALAERLPHLAGLHAPVG
                     RARAYFCREGACEMPLDTPEALRARLTGFPRW"
     gene            2868..4961
                     /locus_tag="PK362_00705"
     CDS             2868..4961
                     /locus_tag="PK362_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73393.1"
                     /translation="MKNKIWRAFDRVLGFPERGMTLVEVLIAFTIVTLVLLGVSLALS
                     NTAKAVFSSKQEEVASRLAQLIHAKLLQVDFDQIFSVDSNNKNPTRTPTPLYYDYETN
                     PTQYNFWAPWSNLALTTRTLTILGQIEQEVRSAGFTRFTITVRHMRRDSSDVDNQQNV
                     SDLTFFARYYGSNLNQVFTVTPNVNAPDDADPNIAFRDRNGDSDFWDVVGGVPETPNT
                     DMKLLTITIYNANRVIAIKHGDLITRQQFSGRASNTTESQLKLIWKVPYQNLWSVNST
                     VFYQRDTLAKQNALDVPFFYQAQIDAARAPKTAGNTATQADHHGADVIRNFHVEGESE
                     PGATIEVEYAGAGVAWGAATKDSCAVMSNGQFACDCPNTSNYIQINPCFFGTCYGAGA
                     YSFSVRLRARSQQGLYSPYVTRGYIVDYFPPVIDNFRPSTTTVSVHTRQPVIACRFGD
                     PGQDGVSGANTWLYNLWYSTTAGSTWAGPFRWDNNMWRFPYPDGLYWSGTYILSNDYA
                     WTTVPANRRGLPFVFNNGQNVIVRAEMCDVATYKATTSWTFQISVDEGDTTGPTVDVS
                     TSNGFNLSKSAIPAAVRDSHLPPMGGWRRLAVLDDPESGIDWGSLSYTWYGPLGSSRT
                     YSAATLTGFQTLEYSGYGCSATGGGCLFDPYNSAFGLPIASMSTGTYNIGLSVQNWAV
                     PPVVTTVTWTFDIVP"
     gene            4958..5800
                     /locus_tag="PK362_00710"
     CDS             4958..5800
                     /locus_tag="PK362_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC73394.1"
                     /translation="MRSNRGFTFVELVVAAAIVGIITPPIVLYVGRSMDHSRKVAAKL
                     TAQDSTRAAMDQIELDITDMNDLLVAQSERIEFLMDSNRKTGYNPNLLTARGVSQRFD
                     PDDDGDWETPPANLSILPRNSPTTFQSYGNDLDDDDDDNDGNADVRCVVYRSNGQLIR
                     ELYFNKSLASTTPDVQKVLIAHCTRLAFSFAGSVNENRGNTGLDANGDGLITDSEIDD
                     APQYATLEGGNGNGAVDTRSERAYVTNIGVALGEDTNVDGQEDSALKTEIYPPLLPYK
                     NKYP"
     gene            5833..>6271
                     /locus_tag="PK362_00715"
     CDS             5833..>6271
                     /locus_tag="PK362_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73395.1"
                     /translation="MMIALILIVFVLPIVTVLVKSVFRQSKTNFQERNMKTARVVSND
                     IMINFMRVHSGNYYEDHYDTRFMLSGDVSNTFLSAGNSGVGTVGYVTGASAVGKYFYV
                     QSTATLVSGADQTVWGKKGMVGVIGFENDLTRFGMIFMNDAIFR"
BASE COUNT         1262 a         2095 c         1859 g         1055 t
ORIGIN      
        1 ggccccgtca acccccgaat ccacgacggc caccaccacc ccgccccccg tgctcgcgtc
       61 ccaggcctcg ggcgcattga ttttcggaag ggcccacagg tcggaataat cggggtcgtt
      121 ggggacccgg aacgcctcaa agcgggcgtc gggttcggcc cagtccacgg cggggtccgc
      181 ggcgtaggct cgggcgacga cttccggatc gtccggccct tcgtaaatga gcacttgaac
      241 gctttcaagg tcccggcgca gggattgttg gagtgtcgac cgggcggggg cgccctgttt
      301 agcggaggcg gcgggggggg ctcgaaaaac agggccgagg gaacgaacgc cgaaggccgc
      361 gctcagtcca ttcaaggccg gggctccgac cacggtgggc gatttcccag cggggctcga
      421 ccggtaacgg acaatcacgc gcttgggccc ctcccccgcg acgcccgggg tcccggcgaa
      481 aagggaggcg gccgggagga gccccaggaa ccatcgggga gcggagcgaa aaaaggcgga
      541 acgacggcgc ctcacggttt caccagcggg gaaacccggt caatcgagcg cgcagggctt
      601 cgggggtgtc caggggcatc tcgcaggctc cttcgcggca aaaataggcc cgggcccgac
      661 cgaccggggc gtgaaggccc gccaaatggg gcagccgttc cgccagggcc gcctggcctt
      721 cggcctggtc cgccaagacg accaacaggt cggggcggaa aacccggcga gcctcggcca
      781 acaggcgccg ggtcgcctcg ttgcccggac ggcccgcgat gaccaattcg cttttggggc
      841 aaaggtccac gaccacggcc gacaaaaacc ccgggaacgc ccggggcgcc tcggtggccc
      901 gaccccgcaa ggcgtcggag atctttccgg ccatcgcggt gaaatccgcg cgggcccgcc
      961 atcggcccag tcgttggaga accgccaccg ccaccgacga ggcgcccggc tccacgttgt
     1021 cgccctcgtc ccgggcccgc acgggcaaac gggggtcctg gtccgggggc gtcgcgtgga
     1081 ccacgcccga ttcccggtcg acgaaggttt gaagaaaaac ctccgcgagg gtttccgccc
     1141 agcggagcca agagctatcg aaggtcgcct cgtacaaatc gaccaggccc tggatcaaga
     1201 aggcgtggtc gtcggcctga ccggccacgg cgcgttcccc gtcgcgccac cggcggtaca
     1261 ggcgccctcc ggcggcgtcc caaagacggc ttttcagaaa ttcagcgcat cgcaccgcgg
     1321 cctcccgata acggtcctcc cccaggatcg cgtggccttt cgccagggcg gagagggcca
     1381 gaccgttcca gcccgtcagt attttgtcgt cgagaaaggg ccgggggcgg gatcgccggc
     1441 ggtcgaacaa cgtcgatcgg gacccggcca acccccggga cacgtcctcg atcgtccacc
     1501 ccccttcggc cgcgagatcc gcctcggacc gggcgcggca aagcacgttt tccccggcga
     1561 actcccccgt gccgtcgtcg acgttgcctt cgggtcgaac cccgtgggcc cgggcaaaga
     1621 cctccgcctc ctccccgaga atcttggaca attccgacca cgtccaaacg tagaaggcgc
     1681 cctccttctt atcggccccc gggggggaat cctcaggccg gctgtcggcg tcttcggcgg
     1741 agaaaaatcc gccctgggcg tttcccaaat cccgcaccag gtaatcgagg gtggatcgcg
     1801 ccgtccgggc cagatccgga tcgcccgtga gctggtgggc ctccaaaaaa ttgaccgcca
     1861 actgggcgtt gtcgtacagc atcttttcaa aatgcggcag gtgccagcga tcgtccgtgg
     1921 aataacggtg gaatccgccg cccaattggt cgcaaatccc ccccgccgcc agggccgcga
     1981 gggtccggac cgccatgtcc cgggccgttt taccccgcgg gtccgccagg gcctcggccc
     2041 gggcggcgaa acgaaggagg aaatgctggt tgacgggcag ggggaatttg ggggcgccgg
     2101 aaaacccgcc gtggtcggga tcgaaggatt cccgaaaggc gtcgaaggct tttgacacgg
     2161 cttcttcggc gtcggcctcg gccacggtgc gcgatccgct tagaaatccg gccaccgccc
     2221 ggaccagctc ctccccttgg cgctccaggg attcgcgttc cggggggttt ttccaggcct
     2281 cgccgatccg ttggacgagc cggggccagc cggggcgtcc accccggtcc gccgggggga
     2341 aataggttcc ccccaaaaaa atcttgcccg tcggggtcaa aaacacgttc aggggccaac
     2401 cgccctgacc cgtcatcgcc tggaccgccg tcatgtatag gcggtccacg tcgggccttt
     2461 cctcccggtc gactttgacg cacaccaggt gccgattcat gacctcggcg atggcggggt
     2521 tttcgaaact ttcccgctcc atgacgtggc accaatggca ggtggcgtac ccgatggaca
     2581 caaggatcgg tttgtcctcc cgaaaagccg tttcgaaggc ttcgtccccc cagggcagcc
     2641 aatggacggg gttggtggcg tgctggagca aataagggct tttttcggtc gataatttat
     2701 tcatggttgg caacaagccg ggactggaaa cgaagccgtg gcgctaaaaa ggcaaaaacg
     2761 taacaaacga aacaattgat tacgaaggga tttggcaatg gatatgaccc agatcatacc
     2821 attgaaatcg gaccgaaggg gccttatact cagatcggag gcgacccatg aaaaacaaaa
     2881 tttggcgcgc gttcgaccga gttctcgggt ttccggagcg gggaatgacc ctggtggagg
     2941 tactgatcgc cttcaccatc gtcaccttgg tgctgctcgg cgtctccctg gccctttcca
     3001 acaccgccaa ggccgtgttt tcctccaaac aagaggaagt ggcctcccgc ctcgctcaac
     3061 tgatccacgc caaactcctg caggtggatt tcgatcaaat tttctccgtg gattccaaca
     3121 acaagaaccc cacccggacc ccgacgcccc tctattacga ttacgaaacg aatccgaccc
     3181 agtacaactt ctgggccccc tggtccaacc tggccctgac cacacgcacc ctgacgatcc
     3241 tgggacaaat cgaacaggag gttcgcagcg ccgggttcac ccggttcacc atcaccgtcc
     3301 gccacatgcg gcgggattcc tcggacgtgg acaaccaaca gaacgtttcg gacctgacct
     3361 ttttcgcccg ctattacggc tccaatttaa accaggtctt cacggtcacg ccgaacgtca
     3421 acgccccgga cgacgccgac cccaacatcg ccttccggga ccggaacggg gacagtgatt
     3481 tctgggacgt ggtgggcggc gtgcccgaaa cgcccaacac ggacatgaaa ctcctcacca
     3541 tcaccatcta caacgccaac cgggtcatcg ccatcaaaca cggcgacctc atcacgcgcc
     3601 agcaattctc cggccgggcc tcgaacacga cggaatccca gttaaaactc atctggaaag
     3661 tcccctacca aaatctgtgg tcggtcaatt cgacggtttt ttaccaacgg gacaccctgg
     3721 ccaaacagaa cgccctcgat gtgcccttct tttaccaagc gcaaattgac gcggcccggg
     3781 cccccaaaac cgccggcaac accgccaccc aggcggacca ccacggggcg gacgtgattc
     3841 gaaatttcca cgtggagggc gaaagcgagc ccggagcgac cattgaagtg gaatacgccg
     3901 gcgcgggggt ggcctggggc gccgccacca aggacagttg cgccgtgatg tcgaacggcc
     3961 aatttgcctg cgattgcccg aacacgtcca attacattca gatcaacccc tgctttttcg
     4021 gcacctgtta cggcgcgggg gcctattcct tttccgtgcg tttgcgggca cgctcccaac
     4081 aggggcttta ctcgccctac gtgacccgcg gctacatcgt ggactatttc cccccggtca
     4141 tcgataattt ccgaccgtcc accaccaccg tgagcgttca cacccggcaa ccggtcatcg
     4201 cctgccggtt cggcgacccc ggacaggacg gcgtgagcgg cgccaacacg tggctttaca
     4261 acctgtggta ttcgaccacc gccggatcca cctgggcggg gcccttccga tgggacaaca
     4321 acatgtggag attcccctat cccgacggcc tttattggag cggcacctac atactgtcca
     4381 acgactacgc ctggaccacg gtgccggcca atcggcgcgg attgcccttc gtgttcaaca
     4441 acggtcaaaa cgtgatcgta cgagcggaaa tgtgcgacgt ggcgacctac aaggccacca
     4501 cctcctggac gtttcaaatc agcgtcgacg aaggcgacac gacgggaccc accgtcgacg
     4561 tgagcaccag caacggattc aacctgtcga agtcggccat tccggcggcc gtccgggatt
     4621 cccatttgcc gcccatgggc ggttggcgcc gcctggcggt cctggacgat cccgaaagcg
     4681 gcatcgactg gggaagcctg agctacacct ggtacggacc gctcgggagc tcccgaacct
     4741 acagcgcggc aaccctcacc ggattccaaa ccctggaata ctccgggtac gggtgctcgg
     4801 ccacgggcgg cggatgcctc ttcgatccct acaacagcgc cttcggcctt cccatcgcca
     4861 gcatgtcgac cgggacctac aacatcggcc tgtcggtgca aaattgggcg gtcccgcccg
     4921 tggtcacgac cgtgacgtgg actttcgaca tcgtcccatg aggtcgaacc ggggattcac
     4981 gttcgtggaa ctcgtggtgg cggccgcgat cgtcggcatc atcacgccgc ccatcgtttt
     5041 gtacgtgggg cggtccatgg accattcccg caaggtggcg gccaagttga ccgcccagga
     5101 cagcacccgc gccgccatgg atcaaatcga attggacatc acggacatga acgatttgtt
     5161 ggtcgcgcaa agcgagcgga tcgaattcct gatggattcc aaccgaaaga cgggctacaa
     5221 cccgaatctt ttgacggccc ggggcgtgtc ccaacggttc gaccccgacg acgacgggga
     5281 ctgggaaacc cccccggcca acctgtccat ccttccgcgc aattccccca cgacctttca
     5341 atcgtacggt aacgacctgg acgacgacga tgacgacaac gacggcaacg ccgacgttcg
     5401 atgcgtggtt taccgctcca acggtcaatt gatccgtgaa ctgtatttca acaaatcgct
     5461 ggcgtccacg acgcccgacg ttcagaaagt cctgatcgcc cactgcaccc gcctcgcctt
     5521 ttccttcgcc ggctccgtca atgaaaaccg cggaaacacc gggctggacg ccaacggcga
     5581 cggcctcatc accgattccg aaatcgacga cgcgccccag tatgccaccc tggaaggcgg
     5641 caacggcaac ggcgcggtgg acacccgctc ggaacgggcc tacgtcacca acatcggggt
     5701 cgccctgggc gaagacacga acgtcgacgg gcaggaggat tccgcgctca aaacggagat
     5761 ttaccccccc ctcctcccct acaaaaacaa atacccatga tctcccgccg ccgaaaaccc
     5821 agaggatccg ccatgatgat cgccctgatc ctcatcgtgt tcgtcctgcc catcgtgacg
     5881 gtcctggtca aatccgtttt ccgccaatcc aaaacgaatt tccaggaacg gaacatgaag
     5941 acggcccggg tcgtgtccaa cgacatcatg atcaatttca tgcgggtcca cagcggcaat
     6001 tattacgagg accattacga cacccgtttc atgctgagcg gggacgtttc gaacaccttc
     6061 ctgagcgcgg gcaacagcgg ggtgggaacc gtgggatatg tcacgggcgc cagcgccgtg
     6121 gggaaatatt tttacgttca gagcaccgcc accctggtgt ccggagcgga ccagaccgtg
     6181 tggggaaaga agggcatggt cggggtcatc gggtttgaaa acgacctgac ccggttcgga
     6241 atgattttca tgaacgacgc tattttccga t
//
LOCUS       DAOQLS010000021         6258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5346_length_6258_cov_4.776882, whole genome shotgun sequence.
ACCESSION   DAOQLS010000021 DAOQLS010000000
VERSION     DAOQLS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5346_length_6258_cov_4.776882"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK362_00720"
     CDS             <1..203
                     /locus_tag="PK362_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73396.1"
                     /translation="LLGWESLTARARRAHPTPDHFWPLLYVAALRRPGEPLSFPVEGF
                     QNGTIAMRAVQVGAAPPTVSRH"
     gene            266..1438
                     /locus_tag="PK362_00725"
     CDS             266..1438
                     /locus_tag="PK362_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC73397.1"
                     /translation="MKKVIFRRYLLKRLNTKISDNKKLALLHGTLRRYCPFVDRIAIA
                     LHDTKAGRLKTYLSSNRGPSPLVRYESALDDSPSLRRLLANRRSRVVNDLSVFDEGRG
                     LHTLEIRAHGYKSSYAFPMVVNGRVAGLLFFNSRKKNSFTPRSLDVLDLFAHLITNVA
                     TAGLRTAGVMTAALQTANEMVHYRDPETGNHLERMARYSRLVAQELARTGLYRFDDEQ
                     IQTIESFAPLHDVGKLGIPDNVLLKPGSLTPKERKIMRSHTTQGRRIIDAILHNFKLE
                     SLHRVDALRHIAEHHHEAMDGSGYPHGLKGNKIPIEARIIAVADIFDALTSVRPYKSA
                     WTNDRAFRYLRRLSRTKLDRDCVQALLHNRHAVEDIQARFRDSQAPAPAGRPDDRR"
     gene            complement(1470..2408)
                     /locus_tag="PK362_00730"
     CDS             complement(1470..2408)
                     /locus_tag="PK362_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNC73398.1"
                     /translation="MLDPVILFFVLGLLAGGAKSDLRLPEPVYKMLSIYLLVTIGLKG
                     GVHLAEHAGSGLIGPVVAALLLALIIPAIAFGVLKVWGKFSRDDAAALAAHYGSVSAV
                     TFAVGQAFLDRRGLPHEAYIALLVAVMEVPAIGLAVALARRGEGKTEWGPLAVEVLLN
                     RSIFLLIGGVAIGAVAGHERLQPIEGFFFGGFKGALALFLLEMGVLASQRLGDLKKVG
                     PFLVGFGILMPLLAGTLGALAGTLAGLSLGGTTLLAILAASASYIAAPAACHVAIPRA
                     NPTLYLTAALGITFPFNLTVGIPIYFALAGAFHRSG"
     gene            complement(2414..3472)
                     /locus_tag="PK362_00735"
     CDS             complement(2414..3472)
                     /locus_tag="PK362_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNC73399.1"
                     /translation="MLLGGLALLVLGAEFLIRGSSRLALRAGIPSLVVGLTIVAYGTS
                     APEMVVSAKAALAGQADLALGNVVGSNIFNVLFILGASALLASLLVSRPIIQREVPIM
                     IGVSVLLWVLALDGRVSRAEGVFLLLLLGAYTIFQVRLGLRQGNGHPVAVKSSTGLNV
                     LAIVGGLALLVLGARWFLDGSVQLARSAGLSELIIGLTIVAAGTSLPEVATSLLATWR
                     GERDLAVGNVVGSNIFNILGVLGVAAVLGDGVAVSGAARAFDIPLMTAVALACLPIFF
                     TGHVIARWEGIVFLFYYAAYTAFLVLDARGHDALPLFNAVMLNFAVPLTVLTLGIFVY
                     RHWRNPPPSVSATRGNSL"
     gene            complement(3506..4039)
                     /locus_tag="PK362_00740"
     CDS             complement(3506..4039)
                     /locus_tag="PK362_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73400.1"
                     /translation="MLKAFRNQLNQLSLVDRDSWTQRVTSALGGRRDSGTEEDVRGLI
                     LALPDLLERVRRWYDRADVPENFRRLHGITLTYLYHPADLVEDKGLFGYLDDAYLVGR
                     VFVLTAQRLTRDGVALEEDVAARHTTLERGLASARRVLPEETNRLDALVADYLSGDLK
                     AFRRLMDETEKLAVGKQ"
     gene            complement(4065..4928)
                     /locus_tag="PK362_00745"
     CDS             complement(4065..4928)
                     /locus_tag="PK362_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNC73401.1"
                     /translation="MNRLKTTALLGLLTALLLWMGQALGGSTGLAMALLMAGAMNFAS
                     YWWSDKIVLAMSGAKPLGEREAPDLHRMVRTLSIKAAIPVPRLYRLAQAAPNAFATGR
                     DPAHGAVAVTEGLLASLDRAEIEGVLAHEIGHIKNRDTLTMTVAAALGGALSMLADMA
                     LWATLAGRSSSDNDEGGHPLAGLVGVIVAPLAALLIQAAISRSREFDADEAAARLSGN
                     PRALASALLKIEAMAKRKALPTSPTAAHLFIVNPLRGGGLSGLFSTHPATRERVKRLE
                     ALAREGLSWAA"
     gene            complement(4954..5532)
                     /locus_tag="PK362_00750"
     CDS             complement(4954..5532)
                     /locus_tag="PK362_00750"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNC73402.1"
                     /translation="MLKAFQEKLTRLANDPQDPFRQRVTERVGPLEAPRTEAPLRRMI
                     LAMPDMLGQIASWSRELGMPQPLKRAQVFALAYIVNPEDFLPEGASGLYGYLDDAYIV
                     AATYYRTMQEVGPAGLRPLTDNVSLALAVPEWVEAFRRYWPELAERIDDLLDTHLDRP
                     DARRMGNGRWLGSRRPVARTAAVARNRAPRRS"
     gene            5628..>6258
                     /locus_tag="PK362_00755"
     CDS             5628..>6258
                     /locus_tag="PK362_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC73403.1"
                     /translation="MIPLNYHHLFYFYTVAKTGSIAKATETLLLAQPTISAQIKELER
                     FFKRPLFERRNRRLVLTEDGRRVLDYAESIFGLGRELEDVFRDRAPGSPTGAQLGVAT
                     GTPRALAHRLLERALADFPGAPLIVREGSTAALAEDLRQQRLDLVLSTSRLPPGTKDE
                     FITKRVGRIPLVFAAVPRRAAAVRRWPDDLGRQPMVLPAGGAYREVRDLW"
BASE COUNT         1204 a         2076 c         1964 g         1014 t
ORIGIN      
        1 ccctcctcgg ctgggaatct ctgaccgccc gggcccgccg ggcccacccc acccccgatc
       61 atttttggcc ccttctttac gtggccgccc tccggcggcc cggggaacct ctttcctttc
      121 ccgtggaggg gtttcagaac ggcaccatcg ccatgcgggc cgttcaagtg ggggcggcac
      181 cgccgaccgt ttcccgtcat tgacgccggg ggaatttcct gcaagacttt gggggccgcc
      241 aaaaacaccg aggggggcgc gggccgtgaa aaaagtgatt ttccgccgct atctgctgaa
      301 gcggctcaac accaagatct ccgacaacaa aaaactcgcc ctcctgcacg ggaccctgcg
      361 gcgctattgc ccctttgtgg accggatcgc catcgccctg cacgacacga aggccggccg
      421 cctcaaaacc tacctgtcca gcaaccgggg cccctcgcct ctcgttcgct acgaatccgc
      481 cctggacgac tcgccctccc tgcgtcggct cctggccaac cggcggtccc gggtggtcaa
      541 cgacctttcg gtgttcgacg aagggcgggg attacacacc ctggaaatcc gcgcgcacgg
      601 ttacaagtcc agctacgcct tccccatggt cgtcaacggc cgcgtggcgg gactcctgtt
      661 ttttaacagc cgtaaaaaaa acagttttac gccgcgctcc ttggacgtgt tggatttgtt
      721 cgcccacttg atcaccaacg tcgccacggc gggcctgcgc acggcggggg tgatgacggc
      781 ggcccttcaa accgccaacg aaatggtcca ttatcgggac cccgaaaccg gaaaccattt
      841 ggagcgaatg gcccgttatt cccgcctcgt ggcccaggag ctggcccgca ccgggctgta
      901 tcgtttcgac gacgagcaga ttcagaccat cgaatctttc gcgcctctgc acgacgtggg
      961 gaaactcggc attccggaca acgtcttgct caaaccgggc tcgttgacgc ccaaggaaag
     1021 aaagatcatg cggtcccaca ccacccaggg ccgccggatc atcgacgcca tcctccataa
     1081 tttcaaattg gaatccctgc accgggtgga cgcccttcgg cacatcgccg agcaccacca
     1141 cgaggccatg gacgggtccg gctatcccca cgggctgaaa ggcaacaaga tccccatcga
     1201 ggcgcggatc atcgccgtgg ccgacatttt cgacgccttg acgagcgttc gtccctacaa
     1261 gagcgcctgg accaacgacc gggcgttccg ttacctgcgc cgcctcagcc ggacgaagct
     1321 ggatcgggac tgcgtgcagg cgctcctcca caaccggcac gcggtggagg acattcaggc
     1381 ccggtttcgg gattcccaag cccccgctcc cgccggccgc cccgacgacc ggcggtgacc
     1441 cgcggcgtcc cctcgccaag ggggaggccc taccccgaac ggtgaaaggc gcccgccagg
     1501 gcgaagtaga tgggaattcc cacggtcaga ttgaacggga acgtaattcc cagggccgct
     1561 gtcagataaa gcgtcgggtt cgccctcggg atcgccacgt gacaggcggc cggggcggcg
     1621 atgtagctgg cgctcgcggc caagatggcc aagagcgtcg tgccgcccag ggaaaggccc
     1681 gcgagggtcc cggccagggc ccccagggtt ccggccagta gaggcatcag aatcccgaag
     1741 cccaccagga aaggccccac cttttttagg tcccccagtc gctggctggc gagaacgccc
     1801 atctccaaga ggaatagggc caaggccccc ttgaaccccc cgaagaaaaa cccctcgatg
     1861 ggctgaagcc gttcgtgtcc ggcgaccgcg ccgatggcca cgccgccgat caaaagaaat
     1921 atggaccggt tgaggaggac ctcgacggcc aggggccccc attcggtttt gccctcgccc
     1981 cggcgggcga gcgccacggc caaaccgatg gcgggaactt ccatcaccgc gaccaagagc
     2041 gcgatgtagg cctcgtgggg cagtccccgg cgatccagaa aggcctggcc cacggcgaag
     2101 gtcacggcgg acacgctgcc gtagtgggcg gccagggcgg cggcgtcgtc ccgggaaaat
     2161 ttcccccaaa ctttcaacac cccgaaggcg atggcgggga tgatcagggc caggagaaga
     2221 gcggccacga cgggtccgat caatccggag ccggcgtgct ccgccaggtg gaccccgccc
     2281 ttcaggccga tggtgacgag caggtaaatg ctcagcattt tatagaccgg ttccggcagg
     2341 cgcagatcgc ttttggcgcc cccggccaac aggcccagga cgaaaaagag gatgacgggg
     2401 tccagcaagt tgctcacaat gaatttcctc gggtcgccga gacggacggt ggggggttcc
     2461 gccagtggcg gtaaacaaaa atccccaggg ttagaaccgt cagggggacg gcgaaattca
     2521 gcatcaccgc gttaaacagc ggcaaggcgt cgtgcccgcg cgcgtcgagg acgaggaatg
     2581 cggtgtaggc ggcgtagtag aagagaaaga cgattccttc ccaccgggcg atgacgtggc
     2641 ccgtaaagaa aatcggcagg caggccaggg cgacggcggt catcaaaggg atatcgaagg
     2701 cccgggccgc cccggaaacg gccacgccgt ccccgagaac ggccgccact cccagaacgc
     2761 ccaggatgtt gaagatgttc gatcccacca cgttgcccac ggccagatcc cgctcacccc
     2821 gccaggtggc caacagcgag gtcgccactt ccggcaggct cgtgcccgcg gccacaatgg
     2881 tgaggccgat gatgagttcg ctcaaccccg cggaccgggc caattgaacg ctgccgtcca
     2941 ggaaccatcg ggcccccaga acgagaaggg ccaatccacc gacgatcgcg agaacgttga
     3001 gtcccgtgga ggatttcacg gcgaccgggt ggccgttgcc ctggcggagg cccaatcgca
     3061 cctggaagat cgtgtaggcc cccagcagga ggagaaggaa gaccccttcc gcccgggaca
     3121 cccgaccgtc cagggccagg acccagagga gaacgctgac gccgatcatg atgggaactt
     3181 cccgctggat gatgggacgg ctgaccagta aggaggccag cagggccgag gcccccaaga
     3241 taaacagaac gttgaaaatg ttggacccca cgacgttccc cagggcgaga tcggcctgcc
     3301 ccgccaaggc ggctttggcg ctgacgacca tttccggagc gctcgtcccg taggccacga
     3361 tggtcaggcc caccaccagg cttggaatcc ccgcccggag cgccaaccga ctggagcccc
     3421 ggatcaaaaa ctccgctccc aggaccagga gggccagtcc ccccaaaagc atgatcgtcg
     3481 tcatttcggg ttccacgaaa ccattttact gtttccccac ggccaacttt tcggtttcgt
     3541 ccatgaggcg ccggaaggcc ttcaggtctc cggaaaggta atcggccacc agcgcgtcca
     3601 agcggttcgt ttcctcgggc aacacccgcc gggcggaggc gagcccgcgc tccagggtcg
     3661 tgtgccgcgc cgccacgtcc tcctccaagg ccacgccgtc ccgggtcagt cgttgggcgg
     3721 tcaggacaaa aacccgcccc accaaatagg cgtcgtccag atagccgaac aaccccttgt
     3781 cttcaaccag gtccgcgggg tgatacaggt aagtcaaggt gatgccgtga agccgacgga
     3841 aattttccgg tacgtcggcc cgatcgtacc accggcgaac ccgctccagc aggtcgggca
     3901 gggccaggat caacccccga acgtcctcct cggttccgga atcccgtcgt ccgcccaggg
     3961 ccgacgtcac gcgttgggtc caggaatccc gatcgacgag gcttaactgg tttaattgat
     4021 tgcgaaaggc tttcagcatg acatcctcct tgaacgaacc aatctcacgc cgcccaggac
     4081 aggccttccc gggccagggc ttccaaacgc ttgacccgct cccgggtggc cgggtgggtt
     4141 gaaaagaggc ccgacaaacc gcccccccga aggggattaa cgatgaacaa gtgcgccgcg
     4201 gtgggcgacg tgggcagggc ctttcgtttg gccatggctt cgattttgag cagggcgctc
     4261 gccagggccc ggggatttcc cgacaggcgg gccgcggcct cgtcggcgtc gaattcccgg
     4321 gagcgggaaa tggcggcttg gatcaacagc gccgccaggg gggcgacgat caccccgacc
     4381 agtcccgcca gggggtgacc gccctcgtcg ttgtccgagg aagaacgccc cgccagggtg
     4441 gcccacaggg ccatgtcggc cagcatcgac aaggccccgc ccagggccgc cgccaccgtc
     4501 atggtcaggg tgtcccggtt tttgatgtgg ccgatttcgt gggccaacac gccttcgatt
     4561 tccgcccggt ccagggaggc cagaagcccc tcggtcacgg cgaccgcccc gtgggccggg
     4621 tcccgccccg tggcgaaggc gttgggggcg gcctgagcca gacgatagag ccggggaacc
     4681 ggaatggccg cttttatgga cagggtccga accatccgat gaaggtccgg ggcttcccgc
     4741 tccccgaggg gtttcgcgcc cgacatcgcc agcacgatct tgtccgacca ccaataactc
     4801 gcgaagttca tggccccggc catcagaaga gccatcgcca agcccgtcga gccgcccagg
     4861 gcttgcccca tccacagaag caaggcggtc aacaatccca acagcgccgt tgtttttagt
     4921 cgattcatgg aatgatctcc ttccttaagt cggttaagaa cgtcgcgggg cccggtttcg
     4981 agccaccgcc gcggtccgcg cgacgggtcg ccgcgacccc agccaacggc cgttgcccat
     5041 tcggcgggca tccgggcgat ccaaatgcgt gtcgaggagg tcgtcgatcc gctccgccag
     5101 ttcgggccag taacgccgaa aggcttccac ccattcgggc acggcgaggg ccagggacac
     5161 gttgtcggtc agggggcgca ggcccgcggg accgacttcc tgcatggtcc ggtaatacgt
     5221 ggcggccacg atgtaagcgt cgtcgagata cccatagagc cccgacgcgc cctcgggcag
     5281 gaagtcctcc gggttgacga tgtacgccag ggcgaacacc tgggccctct tcaagggctg
     5341 gggcattccc agctcccggg accaagaggc gatttggccc agcatgtcgg gcatggccaa
     5401 aatcatccgt cgaagcggag cctcggtgcg gggggcttcc agcgggccca cccgttcggt
     5461 gacccgttgt cgaaaggggt cctgggggtc gttggccaac cgcgtcaatt tttcttgaaa
     5521 ggctttcagc atgggggtcc tcccttgtgt atttgaaaga ccattttacc ggtcccccat
     5581 tgcatttgtc caatagataa ataaaaatat atatataggt ttaataaatg attcctctga
     5641 actaccacca tctcttttac ttttacacgg tggccaaaac cgggagcatc gccaaggcca
     5701 cggaaacgct tttgctcgcc cagccgacga tcagcgccca gatcaaggaa ctggagcggt
     5761 ttttcaagcg gccgctgttt gaacgccgaa accggcggct ggtccttacg gaggacggcc
     5821 ggcgggtcct ggactacgcc gaaagcatct tcggcctcgg ccgggaattg gaggacgtct
     5881 ttcgggaccg ggcccccggg tccccgacgg gcgcgcagct gggggtcgcc accggcaccc
     5941 cccgggccct ggctcatcga ttgctggaac gggccctggc ggattttccc ggggccccgc
     6001 tgattgtgcg ggagggatcg acggccgctc tggcggagga ccttcgtcag caacggcttg
     6061 atttggtcct gagcacgtcg cggttgcccc ccggaaccaa ggacgaattc atcacgaaac
     6121 gcgttggccg gatccccctg gtgtttgcgg ctgtcccgcg ccgggcggcc gcggtgcgtc
     6181 gttggcccga tgatttggga cgtcagccga tggtgttgcc ggcggggggc gcctatcggg
     6241 aggttcggga cctgtggg
//
LOCUS       DAOQLS010000022         6144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5499_length_6144_cov_4.722779, whole genome shotgun sequence.
ACCESSION   DAOQLS010000022 DAOQLS010000000
VERSION     DAOQLS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5499_length_6144_cov_4.722779"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK362_00760"
     CDS             complement(<1..251)
                     /locus_tag="PK362_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73404.1"
                     /translation="MNRRKAVWAALLWAPTLLRAQDLTLPLAEAEASAVAGSPQLAAQ
                     ADLETAAESRARAQNARRAPALSVDGSFRYFSEIPAFRPA"
     gene            complement(248..631)
                     /locus_tag="PK362_00765"
     CDS             complement(248..631)
                     /locus_tag="PK362_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73405.1"
                     /translation="MIKALGYLGRFVRDHRQLGLSLLEEAVRGDPEVLDFLKANFGRH
                     LAILRGLVDELKSGSGWGDWPAAGVMSFLMSSVGLPSLFVAGLRRAGAKRPYGEPLER
                     VEEAVLTDAAIDRRARAAVRGLDRS"
     gene            complement(662..883)
                     /locus_tag="PK362_00770"
     CDS             complement(662..883)
                     /locus_tag="PK362_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC73406.1"
                     /translation="MTRPSNDLDQRLLAAGRTLLDERGFGGLSVRAAARRARVNPGMF
                     HYHFKTMRAFKRQLLQSLYEEFFRDFSLR"
     gene            complement(976..1167)
                     /locus_tag="PK362_00775"
     CDS             complement(976..1167)
                     /locus_tag="PK362_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008950132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNC73407.1"
                     /translation="MKCNVGGPDRVFRVVVGVGLIAFALIAKNPWGWIGLLPLLTGLF
                     KFCPAYTVCKISTNKQTPQ"
     gene            complement(1275..1844)
                     /locus_tag="PK362_00780"
     CDS             complement(1275..1844)
                     /locus_tag="PK362_00780"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC73408.1"
                     /translation="MLDETSLIQKSRSGDRKAFDGLLRLYQDRLVRLAQKVCAQAPAE
                     ADDVVQDTLLVALKKVKDFRGDSSLGTWLFRVASNLCWMRLRKKRRRATDPLPEGPEG
                     ESRSRGPWASTDPLDDPAARARRNELRRAVGRALMELPADERAVVVLSDVREMKNEEI
                     AGRLGLTVAAVKSRLHRGRRRLRTHFEKK"
     gene            1893..2645
                     /locus_tag="PK362_00785"
     CDS             1893..2645
                     /locus_tag="PK362_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC73409.1"
                     /translation="MKKASRVLWAVLLSVHAGPSWASPSDKSMKSESLRPVVLWGAGL
                     TLVGLTLDRTIQNDIRSEHFIAKTLDHGDYARKGTVMELLGQTQVPLVLSGVLYGGGR
                     WGRNERLERVGRSGAGALLLSGTAVIGLKTLFGRSRPYVDHDADTFHVFHGAAAKNAS
                     FPSGHTTTVFTLAAVVADEYDSRLVDSLAYGLATAVAVGRLYQDQHWASDVVAGAALG
                     IATGKAVRRWDKAGRGGALRTDGRGIYWAWAF"
     gene            2633..3631
                     /locus_tag="PK362_00790"
     CDS             2633..3631
                     /locus_tag="PK362_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC73410.1"
                     /translation="MGVLRRCFLTALLLSPLGAGRFRAGENPPAGTVANGYAYARPGL
                     WTWLKRQVGDQKEFWVRSSRRDQWPLLGIVTVSTLWLTARDQYLYEKGYQLGDQWGVS
                     HDGVQRTFFKTKLWPTNYVVRLNGPYDLGTGLYFLGDGMTHFTVAGSFLAYGLAKDDS
                     RALQTSSQLVEGIVANGFLVQVLKHATGRENPNTGTAKNGKWRLFPNQKDYAKHVNKY
                     DAFPSGHLATAMVTVTVISENYPEHRWIRPLGYTLMTGLAYEMVNNGVHWVSDYPLAI
                     YMGYTFGRIAVHHGRTPLGAWEIKPLWTGSAAGLRMVHRFGGRTRRTGGSAAEATR"
     gene            3680..4450
                     /locus_tag="PK362_00795"
     CDS             3680..4450
                     /locus_tag="PK362_00795"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNC73411.1"
                     /translation="MKWIFLLAFGGIGLGIFIGGIAWGLKRHALAAHGRRAAGRVIEL
                     QESTSVSEVNGRKVASLSYYPVVEFQASDGSTHKFRGSTGSSSPSYEVGSAVELLYMP
                     DNPSNAQIADFSQFWLGPLVLSIFGFVFLGAGIAAFVMIGKSDETFGPSFDQRIHRAM
                     LYDSKRGVKVTATVRALEPVKKMGKTTGLVVVCATIGLDGQERLFRSAPLEVDPGPGW
                     VGRSVEVYVDPYDAKRYYIHLDPLLTPGANGTSSQGTW"
     gene            complement(4476..5210)
                     /locus_tag="PK362_00800"
     CDS             complement(4476..5210)
                     /locus_tag="PK362_00800"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNC73412.1"
                     /translation="MKTATRWTLVLTMFAAPLFGRSEEITADRVVALADAVRNPQEDY
                     QVTAIVSDFSPSAKEPRRAGYQIWVRGREKSVVRTAFPAVERGKSLLMLGRNLWIIIP
                     GTSQPIRVSFQQRLMGEVANGDLSRANFSGDYKAEFLEITDAYYRLQLMAKSDDVTYH
                     KILFWVEKKKYTPMKAEFYSISGRHLKTVTYEGYSRLGGRLRPTRHVIESATVRGQKS
                     IIDFSDMKVGPVDEKYFNKTYLRKMS"
     gene            complement(5213..>6144)
                     /locus_tag="PK362_00805"
     CDS             complement(5213..>6144)
                     /locus_tag="PK362_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC73413.1"
                     /translation="DTDVSPRLISRGETSVSFVGQGVNPRGESKMNTMFRIEAGKNLQ
                     AKDKFQVVMGRGLAASLSAKPGDTAVIVANTVRGSVNALDVTVKGIFSTSAKAFDDHV
                     LRVPFSTAAKLLYTGEVQSLIVLLKDTSKTRSVRRDLEKLFKSRGYATEIQSWEDLAD
                     VYNNSRDFLNRQMGILRWIILIVSVLSVVNTANMAVLERVGEIGTMRAMGTSRWKVMA
                     VFIMEGLLLGLMGGALGALAGVGVARWVSHFGIPMPPPPGSTMEWVAHIRLVPRVFAQ
                     AFAIAVGSSFFSILYPSWKASRLEVAEALRHQV"
BASE COUNT         1151 a         1911 c         1938 g         1144 t
ORIGIN      
        1 gccggacgga aggcggggat ttcggaaaaa tagcgaaacg agccgtccac ggaaagggcg
       61 ggcgcccgcc gcgcgttttg ggcccgggcc cgggattcgg cggccgtttc gagatcggcc
      121 tgggcggcca actgcggcga tcccgccacc gccgaggcct cggcttcggc caggggaagg
      181 gtcaggtcct gggcccgcaa aagcgtcggc gcccagagga gcgcggccca caccgctttc
      241 cgacgattca cgagcggtcc agcccgcgca ccgcggcccg ggcccggcga tcaatggcgg
      301 cgtcggtcaa cacggcctcc tccacgcgtt ccagcggttc gccgtagggg cgtttggccc
      361 cggcgcgccg caggccggcc acaaacaaac tgggaaggcc caccgacgac atgaggaagg
      421 acatcacgcc ggccgccggc caatcgcccc acccgctccc gcttttcaat tcgtccacca
      481 gtccccggag gatggccaaa tggcggccaa aattcgcctt tagaaaatcc aacacctcgg
      541 ggtcgccccg gaccgcttct tccagcaagg aaagacccaa ttggcggtgg tcccggacga
      601 atcggcccag atagccgagc gctttgatca agcgatcccg ggcctcgcct tgcgcttccg
      661 cctagcggag ggagaaatcc cggaagaact cttcgtaaag ggactggagc agctgccgct
      721 tgaaggcccg catggtcttg aagtggtaat gaaacatgcc cggattcacc cgggcccgac
      781 gggcggcggc gcgaacggac aggccgccga acccccgctc gtccagcagg gttcgcccgg
      841 cggccaagag gcgctgatcc agatcgttgg agggacgcgt catttgttaa ttatccgttt
      901 gcataattat acatatgacc agttaatacc gcaagtcccc tcgggcgaaa gaaccgcccc
      961 ggggactcgg cgaaattatt gcggtgtttg cttgttcgtg ctgattttac agacggtgta
     1021 ggcggggcaa aatttaaaaa ggcccgtgag gagggggagc aaaccgatcc acccccaggg
     1081 gtttttggcg atcaaggcga aggcgatcag gcccaccccc accaccacgc gaaacacccg
     1141 atccggacca cccacgttgc atttaatcat tggttcctcc ttgaagtggg gggggattcc
     1201 cccccgaaag ccattggctc tattcttttg gatgaccggc gcgcgccaaa ggattcctcc
     1261 tttcggcgcg ccggtcactt cttttcgaaa tgggttcgaa gccggcggcg accgcggtgc
     1321 aaccggcttt tgacggcggc cacggtcagc cccaagcgac cggcgatctc ttcgttcttc
     1381 atttcccgga catcgcttaa aaccaccacc gcccgttcat ccgccgggag ttccatcagg
     1441 gcccggccca ccgcgcgccg cagttcgttc cgtcgggccc gcgccgcggg gtcgtcgaga
     1501 gggtccgtgg aggcccaggg tccccgggac cggctttcgc cctcgggccc ctccggcagg
     1561 ggatcggtgg cgcggcgacg cttttttcgt aggcgcatcc agcaaagatt ggacgcgacg
     1621 cggaacagcc aggtcccgag agaagaatcc ccccggaaat ccttgacttt tttaagagcg
     1681 accagaagag tgtcctgcac gacgtcgtcg gcctcggcgg gcgcctgggc gcagactttc
     1741 tgggccagtc gaacaagccg gtcctggtag aggcgaagca atccgtcgaa ggctttccga
     1801 tcgccggatc ggctcttttg aattagcgag gtctcgtcaa gcatgggggc ctcattgtat
     1861 catccaacaa caatctccca ggagagttcc ctttgaaaaa agcgagccgg gtcctgtggg
     1921 cggtcctttt aagcgttcac gcgggtccaa gttgggcgtc gccgtccgac aaatcaatga
     1981 aatccgaatc ccttcgcccc gtcgtcctct ggggcgcggg gctgaccttg gtcgggctca
     2041 cgctcgatcg aacgattcaa aacgacattc gctccgaaca tttcattgcc aaaaccctcg
     2101 accacgggga ctacgcccgg aagggcacgg tcatggaatt gctgggccag acccaggtgc
     2161 ccctggtcct ctccggggtt ctctacggcg gcgggcggtg gggacgcaac gaacggttgg
     2221 agcgggtcgg tcgcagcggg gccggcgccc tgttgctcag cgggacggcg gtcatcggac
     2281 tgaaaaccct tttcggtcgg agccggcctt acgtggacca cgacgccgac accttccacg
     2341 tgtttcacgg cgccgccgcc aagaacgcgt cgtttccctc gggccacacg acgacggtct
     2401 ttaccttggc ggcggtggtg gcggacgaat acgattcccg gctcgtggac tccctggcct
     2461 acggcctggc cacggccgtg gccgtcgggc gcctgtatca ggaccaacac tgggccagcg
     2521 acgtggtggc cggcgccgcc ctggggatcg cgacgggcaa agcggtccgc cgatgggaca
     2581 aagccggccg cggcggcgcc ctgcgcaccg atggtcgggg aatttattgg gcgtgggcgt
     2641 tttaaggcgc tgttttttaa cggccctcct tctgtcgccc ctgggcgcgg ggcgctttcg
     2701 cgccggcgaa aacccgccgg ccggaacagt ggccaacgga tacgcctacg cccggccggg
     2761 tctttggacc tggttgaagc gacaagtcgg cgatcagaag gagttttggg tccggtcttc
     2821 tcgccgggat caatggcccc tgttggggat cgtcaccgtc agcacgttgt ggttgacggc
     2881 ccgggaccag tacctctacg agaaggggta tcaactgggc gaccaatggg gcgtctccca
     2941 cgacggggtt cagcggacct tttttaaaac caaactttgg cccaccaatt acgtcgtccg
     3001 cctcaacggt ccctacgacc tgggcaccgg gttgtatttt ctgggcgacg ggatgactca
     3061 cttcaccgtc gcgggaagtt tcctggccta cgggctcgcc aaggacgatt cccgggcgct
     3121 tcaaacttcc agccaattgg tggaaggcat cgtcgccaac ggattcctgg ttcaagtgct
     3181 taaacacgcg accggccggg aaaaccccaa caccggcacc gccaaaaacg gaaagtggcg
     3241 cctgttcccc aaccagaagg attacgccaa gcacgtgaac aaatacgacg cttttccctc
     3301 gggccatttg gccaccgcca tggtcacggt caccgtgatc tccgaaaatt acccggaaca
     3361 ccgctggatc cgcccgttgg gctacacgtt gatgacgggg ttggcctacg aaatggtcaa
     3421 caacggggtc cactgggtca gcgactaccc cctggcgatt tacatgggct acacctttgg
     3481 gcggatcgcg gttcaccacg gacgaacccc cctgggggct tgggaaataa agcccctgtg
     3541 gacgggttcc gcggcgggac tgcggatggt tcaccggttc ggcggaagga cacggcggac
     3601 gggcgggtcg gccgccgaag cgactcggta aaaatcggct cccccccggt cattggcggg
     3661 gcgccgcgag gaggaaggaa tgaagtggat ttttctactg gcctttggcg gcatcggtct
     3721 cggcattttt atcggcggga tcgcctgggg gctcaagcgg cacgccctgg ccgcccacgg
     3781 gcggcgcgcc gcggggcgcg tgatcgaatt gcaagaatcc acctcggtct cggaagtgaa
     3841 cggccgaaag gtcgcgtccc tttcctatta cccggtggtg gaatttcagg cgtcggacgg
     3901 atcgacccac aaattccggg ggagcacggg gtccagttcc ccgtcctacg aagtgggttc
     3961 ggccgtggaa cttttgtaca tgccggacaa tccgtcaaac gcccagatcg ccgatttcag
     4021 ccaattttgg ctcgggcccc tcgtcctctc gattttcggg tttgtttttt tgggagcggg
     4081 catcgcggct ttcgtgatga tcgggaagtc ggacgaaact ttcgggccgt ccttcgacca
     4141 gcggatccac cgggcgatgc tttacgattc caagcgcggg gtcaaagtga cggccaccgt
     4201 gcgcgccctg gagccggtca agaaaatggg gaaaacgacc ggtctggtgg tggtctgcgc
     4261 gacgatcggt ttggacggcc aggagcggtt gtttcgatcg gccccgctgg aagtcgatcc
     4321 cgggcccgga tgggtgggcc gttccgtgga ggtgtacgtg gatccctacg acgcgaaacg
     4381 ctattacata cacctcgacc cgttgttgac gcccggggcg aacggaacgt cgtcccaggg
     4441 aacgtggtga ccggggcccg aggcgccccg gcggtttagg acattttcct caggtacgtc
     4501 ttgttgaaat atttttcgtc cacggggccc accttcatgt ccgaaaaatc gatgatcgat
     4561 ttctggcccc ggacggtggc gctttcgatc acgtggcggg tcggccgaag gcggcccccc
     4621 aggcggctgt acccttcgta ggtcacggtt ttgaggtggc ggcccgagat ggaatagaat
     4681 tcggctttca tcggggtgta tttcttcttt tccacccaga acagaatttt gtgataggtg
     4741 acgtcgtcgc ttttggccat cagctgcaac cgataatagg cgtcggtgat ttccaaaaac
     4801 tccgctttgt aatcccccga aaaattcgcc cgggacagat cgccgttggc cacctcgccc
     4861 atcagccgct gctggaacga aacgcggatg ggttgggacg tgcccgggat gatgatccag
     4921 agattccgac cgagcatgag caggcttttt ccccgctcca cggccggaaa agccgtccgg
     4981 acgaccgatt tctcccgtcc ccgaacccaa atctggtacc ccgcccgccg cggttctttg
     5041 gccgagggcg aaaaatcgct cacgatcgcg gtgacctggt aatcctcctg ggggttgcgc
     5101 acggcgtcgg ccagggccac cacccggtcg gcggtgattt cctccgaacg cccgaaaaga
     5161 ggggcggcga acattgtgag aacgagagtc caacgggttg cggttttcat ggtcacacct
     5221 ggtggcggag ggcctccgcg acttcgagtc gggacgcctt ccaactggga taaaggatcg
     5281 aaaaaaagga cgagcccacg gcgatggcga aagcctgggc gaagacccgg ggcacgaggc
     5341 gaatgtgggc cacccattcc atggtcgatc cgggcggggg cggcatggga atgccgaaat
     5401 gggacaccca tcgcgcgacc ccgacgccgg ccagggcccc caaggccccg cccatgaggc
     5461 ccagcaaaag gccttccatg atgaacacgg ccatgacttt ccatcgactg gtccccatgg
     5521 cgcgcatggt gccgatttca ccgacgcgct ccaggaccgc catgttcgcc gtgttgacga
     5581 cgctcagaac ggacacgatg aggatgatcc atcggaggat gcccatctgc cggttcaaga
     5641 aatcccggga attgttgtag acgtccgcca agtcttccca ggactgaatt tcggtggcgt
     5701 acccccggga cttaaagagt ttttccaggt cccgccgaac ggaccgggtt ttcgatgtgt
     5761 ctttcaagag aacaatcagg gactgcacct ccccggtgta cagcagcttc gcggcggtgg
     5821 aaaaaggcac tcgcagaacg tggtcgtcga aggctttggc gctcgtggag aaaatccctt
     5881 tgaccgtgac gtccagcgcg ttgaccgacc cgcgaacggt gttggccacg atcacggccg
     5941 tgtccccggg cttggccgaa agggaagccg cgagcccccg gcccatgacc acctggaatt
     6001 tgtccttggc ctgcaaattc ttgccggctt cgatccgaaa catcgtgttc atcttgcttt
     6061 cgccccgggg attgacgccc tgccccacga aggacaccga cgtttctccc cggctgatca
     6121 gccggggaga aacgtcggtg tcct
//
LOCUS       DAOQLS010000023         6136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5511_length_6136_cov_3.506167, whole genome shotgun sequence.
ACCESSION   DAOQLS010000023 DAOQLS010000000
VERSION     DAOQLS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5511_length_6136_cov_3.506167"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PK362_00810"
     CDS             <1..590
                     /locus_tag="PK362_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNC73414.1"
                     /translation="AMRRSLLIAIALATAACAKKIVVQAPPPAEPPIMMLYSDPRASR
                     VGDLVTVQIMETSKGSRRVTSRADKDGELSVDLKTNTGGKAARSSMGLKTTNDTNAES
                     GLERRGSLIANTTARVTEILPGGLLRLEGEQTIVLEGGEQTIRLKGLARPADIGPGNT
                     ILSTRLADARIEYKSRKEPSVHRRGLLAWLVGIVF"
     gene            603..1601
                     /locus_tag="PK362_00815"
     CDS             603..1601
                     /locus_tag="PK362_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNC73415.1"
                     /translation="MNRRWAVLLGIAGTLNLAAGETRVKDVARVGGARDLELVGYGLV
                     TGLRGTGDRPGSGPAAQSLVNLLNRLGVAASPQELQSSNIAAVAVTARVPAFSRPGAA
                     SDARVSSLGNASRLEGGTLLLTALLGVDGKAYATAQGRLAEEPEAGGDRRGAGSAGGA
                     TTAFLPSGVLLEKPLTGPAAETDGRLAVTLDRPDAVTAERIAQAVQETLKTPSRATDP
                     GRVEVEIPEDFREDPVGFAARVERVTVTPDDAPRVVLNERTGTVIAGQGVRIAPVAIS
                     HGGVKIEVGGGPARAEADVTSLVDLLSGLGVRPRDVVVIFQMLKRLGALKAELVLM"
     gene            1655..1918
                     /locus_tag="PK362_00820"
     CDS             1655..1918
                     /locus_tag="PK362_00820"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNC73416.1"
                     /translation="MKQACRGFESLFLHTLLKEMRSTEAPAALEGSGPARDITRDLYD
                     QALSEEMSRAGGLGVGPLLYRQLNRAQVPAPGADKESERRIHP"
     gene            1915..2217
                     /gene="flgM"
                     /locus_tag="PK362_00825"
     CDS             1915..2217
                     /gene="flgM"
                     /locus_tag="PK362_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNC73417.1"
                     /translation="MIPSIGSSGPQGSRGASAADETAKAAPAKKLPARVPPAEPQDQV
                     TLSPLARDLQILRAHANDGAAERAAKVSALKERVARGDYRVSTDDLAARIRDAWSA"
     gene            2231..2725
                     /locus_tag="PK362_00830"
     CDS             2231..2725
                     /locus_tag="PK362_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNC73418.1"
                     /translation="MAADTPLARMESALEDITRLCGELRDLLTEERAALVAARRDALP
                     GLLRKKADGLQRLAEADGRRRRCALQLERSWGWAPVDRPLSQLASRLDGPTRERLAGK
                     KDRLLRVMRDLCALNEANGRLIRQAVTVGRRLLEWATAPRDLIYGPAGIRPDEAGRVV
                     HQRV"
     gene            2757..4385
                     /gene="flgK"
                     /locus_tag="PK362_00835"
     CDS             2757..4385
                     /gene="flgK"
                     /locus_tag="PK362_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNC73419.1"
                     /translation="MSLLASFEIGTRSLRTFQTAIQTVSHNISNVNTPGYSRQRIVLT
                     TEHPDRTDRFDVGRGVRMMGVERLRDAFFEASWRRETTGQGQAEALATAFKSAEALFP
                     EPNGDGLSTRMDKFWSAWQELANHPTDPGIRAVVLTRGADLAAGLNQTLQGIAQQRAD
                     ADSEIAAAVPQINSTAREIYQLNDSIKTALLNGQTPNDALDRRDFLVGQLSQWTSIQV
                     VDHADGSVAVYAGGAPLVEEGRVGSLETLPRAGDPEGHLEIRWKETGDVFEPIGGRLG
                     GWTTARDVVLPGLAQSLADWSRTLRDEVNALHRTGVGLDGSTSLRGAVNFTGTLAADA
                     TVSLNGVDIALNAGDDLATVVSRLNAQEAATGVHAAVDGARLVLAPGGATPQTVHVTG
                     DPDGAWKSLGIVNDFFAGEPGALALSDAVRNNPDAVAASASGAPGDNTLARALAGLRD
                     KKALSGGTTSFDGSYQGFLADLGARSAAAQASQENQSFLLQQIDQLRESVSGVSLDEE
                     LTDLIRYQRSYEMAARSIQTADEMLGTLLDMVHR"
     gene            4398..5273
                     /gene="flgL"
                     /locus_tag="PK362_00840"
     CDS             4398..5273
                     /gene="flgL"
                     /locus_tag="PK362_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNC73420.1"
                     /translation="MRVTERMIHRGVVDEIHGHQAAMAKLQQQIASGQRLTGLSEEPI
                     DAARSIRLRAAAAGLDQYKKNTQEALAWMNATLGGLGRVREVLQEARLDALQGGTDST
                     PPESRQALAETVARLRESLVELGNSKWEDASLFGGHATTATPFAPDGTYRGDTGAIGR
                     DIAPGETTTVNYNGDEVFQSGTNLFAALDNLETALRNNDPAAVRAAVPALDAGIGKIL
                     RFEASLGAETERVALTRSRLDEMSVTLAQRRSENDDTDMAQAAVDLQNENNVYEASLA
                     ASARLFGASLLDFLK"
     gene            5270..5734
                     /gene="fliW"
                     /locus_tag="PK362_00845"
     CDS             5270..5734
                     /gene="fliW"
                     /locus_tag="PK362_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNC73421.1"
                     /translation="MTRRPSVLKSTRFGVLRPDAGSILTLIDGLVGFPDQRRFLLVDH
                     AGREDFGWLQSLEDGGLAFPVTDPRRHWPECRYAFSADDLRGLDLAPGEDPDVLVTVS
                     VPAGGGLRLNLGGPIVFNFRRRRGRQLVQAPGAPVPAALPCSFSPVEKAKAS"
     gene            5695..6009
                     /gene="csrA"
                     /locus_tag="PK362_00850"
     CDS             5695..6009
                     /gene="csrA"
                     /locus_tag="PK362_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNC73422.1"
                     /translation="MLLLSRRKGESLLIGDTVEITVLAVEGGNVRLGIRAPGAVPVYR
                     RELYEAIRSENRDSAAAGVEDLSRALARRPVTPRSAAPAPVRRPRRGDRPSGPSDGPL
                     PS"
     gene            complement(5918..>6136)
                     /locus_tag="PK362_00855"
     CDS             complement(5918..>6136)
                     /locus_tag="PK362_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73423.1"
                     /translation="TKPWNDDEVLAVVSHAVDRFWLMRENDLLKQELELKDAELRKFK
                     KEAGRPKVPKADRPGGAAGPVPAPPTSA"
BASE COUNT         1022 a         2129 c         2014 g          971 t
ORIGIN      
        1 gggccatgag gcgctctctg ttgatcgcca tcgccttggc caccgcggcc tgcgccaaaa
       61 aaattgtcgt ccaggctccg cctccggcgg aacccccgat tatgatgctg tattccgatc
      121 cgcgggcgtc gcgggtgggc gatttggtga cggtgcagat catggaaacg tccaaaggtt
      181 cccggcgcgt cacctcccgg gccgacaagg acggggagct ctccgtggat ttgaagacga
      241 acacgggcgg caaagccgcc cgctcctcca tggggctcaa gacgaccaac gacaccaacg
      301 ccgaatccgg cctggagcgg cggggttccc tgatcgccaa caccacggcc cgggtcaccg
      361 aaattttgcc cggcgggctg ttgcgcctgg agggcgaaca aaccatcgtg ctggagggcg
      421 gcgaacaaac cattcggctc aagggtctcg cccgccccgc ggacatcgga ccgggcaaca
      481 cgatcctgtc cacccgcctg gcggacgccc gcatcgaata caaaagccgc aaggaaccct
      541 ccgtccaccg ccgggggctg ctggcctggc tggtcgggat cgtcttttaa cggggccctt
      601 gaatgaatcg acgctgggcg gttctccttg ggattgccgg aaccctgaat ctcgccgcgg
      661 gggaaacccg cgtgaaggac gtggcccggg tcggcggggc gcgtgacctg gaactggtgg
      721 gctacggtct ggtgacgggc cttcggggaa cgggggaccg gccggggtcc ggaccggcgg
      781 cccaatccct cgtcaattta ctgaaccgat tgggcgtggc ggcgtccccc caggaacttc
      841 aatccagcaa catcgcggcg gtggcggtca cggcccgggt cccggccttc agccgccccg
      901 gcgcggcctc ggacgcccgc gtttcctctt tggggaacgc ctcccgattg gaaggcggca
      961 ccctgcttct cacggccctg ttgggcgtcg acggcaaagc ctacgcgacg gcccaggggc
     1021 gcctggccga agagcccgag gccgggggcg atcgcagggg ggcggggtcc gccgggggcg
     1081 ccacgacggc ctttcttccc agcggggttc tcctggaaaa acccctgacg gggccggccg
     1141 cggaaaccga cggccgcctg gccgtgacgc tggaccggcc cgacgccgtg accgcggaac
     1201 gcatcgccca ggcggtgcag gaaacgctga aaaccccctc ccgggccacg gacccgggtc
     1261 gggtggaagt ggaaattccg gaggattttc gggaagatcc ggtgggcttc gcggcgcggg
     1321 tggaacgcgt gacggtcacg cccgacgacg cgccccgggt tgtcttgaac gagcgcacgg
     1381 gcacggtgat cgccgggcag ggcgtccgaa tcgccccggt ggccatttcc cacggcggcg
     1441 ttaaaatcga ggtcggcggc ggcccggccc gggccgaagc cgacgtcacg tcgttggtcg
     1501 atctgctgag cggcctcggg gtacgacccc gggacgtggt ggtgattttt caaatgctca
     1561 aacgactcgg cgcgctcaag gccgaactgg tgttgatgtg aaactcgatc gaaccgcccc
     1621 ccccgccgct acgccgcccc gggccgacga aaggttgaaa caagcctgcc ggggtttcga
     1681 gagtcttttt cttcacacgc ttttaaaaga aatgcggtcc acggaagcgc ccgcggccct
     1741 cgaggggtcc ggtcccgccc gggacatcac ccgggatctg tacgaccaag ctctgagcga
     1801 ggagatgtcg cgcgccggcg gcctcggcgt gggccccctg ctttaccgcc agttgaaccg
     1861 ggctcaagtt cccgcgcccg gggccgataa agaaagtgag aggaggatac atccatgatt
     1921 ccatccattg gttcctcggg gccccagggc tcccggggcg cgtccgccgc cgacgaaacg
     1981 gccaaagcgg cgcccgccaa gaaactcccg gcgcgcgttc ccccggccga accccaggat
     2041 caagtgaccc tgtcgccctt ggcccgggac cttcagattc tgcgcgccca cgcgaacgac
     2101 ggcgccgccg aacgggcggc caaagtgtcc gcgttgaagg aacgcgtggc ccgcggcgac
     2161 taccgcgtga gcaccgacga cttggcggcc cggattcggg acgcctggtc cgcctgattc
     2221 gttgacggcc gtggccgccg acacccccct ggcgcggatg gaatccgcgt tggaagacat
     2281 cactcgtttg tgcggggaac ttcgtgacct tctcacggag gaacgcgccg ccctggtggc
     2341 cgcccgccgg gacgcactgc cgggactcct ccgcaaaaaa gccgacggcc ttcagcggtt
     2401 ggccgaggcc gacgggcgac gccgccgctg cgcccttcaa ctggaacggt cctgggggtg
     2461 ggccccggtg gatcggcccc tgtcccagtt ggcgtcccgc ctggacggtc ccacccggga
     2521 acggctggcc gggaaaaaag accgcctcct tcgggtgatg cgggacttgt gcgctttaaa
     2581 cgaggccaac ggacggctga tccgccaggc cgtcaccgtg ggccgccgcc ttctcgaatg
     2641 ggccaccgcg ccccgggatt tgatctacgg acccgcggga attcgccccg acgaagccgg
     2701 gcgcgtcgtc catcagcgtg tttaaccgcc cccgcgtccc ggccggggct tgacccatgt
     2761 cccttctcgc cagttttgaa atcggcaccc gctcccttcg cacctttcaa accgcgatcc
     2821 agacggtgtc ccacaacata tccaacgtca acacgccggg ctattcgcgg caacggatcg
     2881 tcctgaccac ggaacacccc gaccggacgg accgtttcga tgtcgggcgc ggggttcgaa
     2941 tgatgggcgt ggaacggctg cgagacgcct tttttgaggc cagctggcgg cgggaaacca
     3001 cgggacaggg acaggccgaa gccctcgcca ccgccttcaa atcggcggaa gcccttttcc
     3061 ccgaacccaa cggggacggc ctgtcgaccc gcatggacaa gttttggtcc gcctggcagg
     3121 aactggcgaa ccaccccacg gatcccggta tccgcgcggt ggtgttgacc cggggggccg
     3181 acttggccgc cgggctcaac cagacgctcc agggcatcgc ccaacagcgg gcggacgcgg
     3241 acagcgaaat cgccgccgcc gttccgcaaa tcaattccac cgcccgggaa atttaccaat
     3301 tgaacgattc catcaagacg gccctcttga acgggcaaac ccccaacgac gccctggacc
     3361 gccgggactt cctcgtcggc caattgtccc agtggacctc gatccaggtc gtggaccacg
     3421 cggacggatc ggtggcggtc tacgcggggg gcgccccctt ggtggaggag ggccgcgtcg
     3481 gatccctgga aacgctcccc cgggccggcg accccgaagg ccatttggag atccgttgga
     3541 aggaaaccgg ggacgttttc gaaccgatcg gcggacgttt gggcggctgg acgacggctc
     3601 gggacgtcgt cctcccgggg ttggcccaat ccctcgccga ttggtcccgg accctgcggg
     3661 acgaggtcaa cgccctgcac cgaaccggcg tggggctgga cggctccacg tcccttcggg
     3721 gggccgtgaa tttcacgggt accctggcgg ccgacgccac cgtatcttta aacggggtgg
     3781 acatcgctct gaacgccggc gacgatttgg ccaccgtcgt ctcccgactg aacgcccagg
     3841 aggccgccac cggggtccac gccgccgtgg acggagcgcg gctcgtgctc gcgccgggcg
     3901 gcgcaacccc ccaaaccgtc cacgtgacgg gcgaccccga cggcgcctgg aaatccctgg
     3961 gcatcgtcaa cgattttttc gccggggaac ccggcgccct ggccctttcc gacgccgtgc
     4021 gcaacaaccc cgacgccgtg gcggcttcgg cctccggggc tccgggcgat aacacgctcg
     4081 cccgcgccct ggccggcctt cgggacaaaa aagccctgtc cgggggcacc accagcttcg
     4141 acgggtccta ccaggggttt ctggccgatt tgggcgcccg ctcggccgcc gcccaagcct
     4201 ctcaggaaaa ccaatcgttt ctccttcagc agattgacca attgcgggaa tccgtctccg
     4261 gggtttccct ggacgaagaa ttgacggatt taatccgtta ccagcgctcc tacgaaatgg
     4321 cggcgcgctc catccaaacc gccgacgaaa tgttgggcac cctgctcgac atggtccacc
     4381 gataaaggga ggtttccgtg cgcgtgaccg aacgtatgat ccaccgcggc gtcgtggacg
     4441 aaatccacgg ccatcaggcc gccatggcca aacttcaaca gcagatcgcc tcgggccagc
     4501 gtctcaccgg cctgtccgaa gaacccatcg acgccgcccg gtccatccgc ttacgggccg
     4561 ccgccgcggg gctggaccaa tacaaaaaga acactcagga agccctggcc tggatgaacg
     4621 ccaccctggg ggggctcggc cgcgttcggg aggttcttca ggaagcccgt ctcgacgccc
     4681 ttcaaggcgg gaccgattcc accccgccgg aaagccgcca ggctctggcg gaaaccgtgg
     4741 cccgattgcg cgaatccctg gtggaactgg gcaattccaa atgggaagac gcttcgctct
     4801 tcggtgggca cgcgacgacg gcgaccccct tcgcgcccga tggaacgtat cggggcgaca
     4861 ccggcgccat cggtcgcgac atcgcgccgg gcgaaacgac gacggtcaat tacaacggcg
     4921 acgaggtgtt ccagagcggc accaacttgt tcgccgcctt ggacaatctg gagaccgccc
     4981 tgcggaacaa cgaccccgcg gcggtgcgcg ccgcggtccc ggcgctggac gcgggcatcg
     5041 gcaagattct tcggttcgaa gcgtccctgg gcgccgaaac ggagcgcgtc gccctcaccc
     5101 gatcgcggct ggacgagatg agcgtgacgc tcgcccagcg gcggtccgaa aacgacgaca
     5161 cggacatggc tcaggcggcc gtggacctgc aaaacgaaaa caacgtctac gaggcgtccc
     5221 tggcggcgtc ggcgcgcctg ttcggcgcgt ccctgttgga tttcctcaaa tgacccgccg
     5281 cccttcggtc ctgaaatcga cgcggttcgg ggttctccgt cccgacgccg gttcgattct
     5341 gacgctgatc gacggactgg tgggttttcc cgaccagcgg cggttcctcc tggtggacca
     5401 cgccggccgg gaggacttcg gctggttgca aagcctcgag gacggcggcc tggcttttcc
     5461 cgtgacggac ccccgccgcc attggcccga gtgccgttac gcgttttccg cggacgattt
     5521 gcgcgggctc gacttggccc cgggcgaaga ccccgacgtt ctcgtgacgg tgtccgtgcc
     5581 ggccggcggc ggtctgcgcc tcaatctcgg cgggcccatc gtcttcaatt tccgccgacg
     5641 ccgggggcgc caactggtcc aagcgcccgg cgcgccggtc cccgcggccc tcccatgctc
     5701 cttctctccc gtcgaaaagg cgaaagcctc ctgatcggcg acaccgtgga aatcacggtg
     5761 ctcgccgtcg aaggcggaaa cgtgcgcttg ggcattcgcg cccccggcgc ggtgcccgtt
     5821 taccggcggg aactctacga agccattcgt tccgaaaacc gggattcggc cgccgcgggg
     5881 gtggaggatt tgtcccgggc tctggcccgc cgcccggtca cgccgaggtc ggcggcgccg
     5941 gcaccggtcc ggcggccccg ccggggcgat cggccttcgg gaccttcgga cggcccgctt
     6001 ccttcttgaa tttacggagc tccgcgtcct tcaattccaa ttcctgtttt aacaggtcgt
     6061 tttcccgcat cagccaaaaa cggtcgaccg cgtgggaaac gaccgccaac acttcgtcgt
     6121 cgttccaggg cttggt
//
LOCUS       DAOQLS010000024         6019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5679_length_6019_cov_3.663649, whole genome shotgun sequence.
ACCESSION   DAOQLS010000024 DAOQLS010000000
VERSION     DAOQLS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5679_length_6019_cov_3.663649"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..2532
                     /locus_tag="PK362_00860"
     CDS             4..2532
                     /locus_tag="PK362_00860"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNC73424.1"
                     /translation="MEFESLSQSAAETRRRAEGPNELPSSHRRGWVVLALDVFREPMF
                     LLLVACGALYFALGDGQEAAMLLGFVFFIMGITLFQEGKTERALEALRDLSSPRALVI
                     RDGSQRRVAGRDVVRGDLLVLAEGDRVPADGILLSSSHLAVDESLLTGESVSVRKEVR
                     AAIPGETPTTSGDGSPYVFAGTLVVQGQGLVRVTGVGAATEIGKIGRALESAESGLSP
                     LQIEMAALVKVVAVVGAILCAAVVLVTGFWRGAWLTGVLAGLSLAMALMPNEFPVVLT
                     IFLSLGAWRLSKRGVLARRMPAVEALGAVTVLCVDKTGTLTENRMDIQRLLPGDLRFD
                     ISPAGNTLEEAFHEILEFGILASARNPFDPMEKAFHALGDRFLARTEHLHGDWTLVKE
                     YPLSRELLAMSQVWTSPDQKRFVIAAKGAPEAVADLCHFPPDRLEALRRKVDEHAREG
                     YRLLGVAKSSFTAEVLPDLQHDFDFEFLGLIALADPVRRDVPAAIEECRRAGVRVAMI
                     TGDYPGTAQSVARAIGLSNADRVLTGPEIDTLSEEALAERLRHVNVFARAVPEHKLRL
                     IRALRANGEIVAMTGDGVNDAPALKAAHIGVAMGERGTDVAREAAGLVLLHDDFASLV
                     GSIAGGRRVFDNLKKGMAYILAIHVPIAGLSLASVFLGWPLVLLPIHIAFLHLVIDPA
                     CSVAFEAEPPEPDAMQRPPRKTTDRLFGWDTLGPSLAQGVSVFTAVFAVFALALRRGQ
                     GELDARALAFTTLMAANAGLILANRSWTQSTWARWRAPNRALWAVVGGSALFLAVVLS
                     TPLLRRLFHFSTLHTIDIAASVAVGTFSVFLVDWTAKRWARRRP"
     gene            2529..3215
                     /locus_tag="PK362_00865"
     CDS             2529..3215
                     /locus_tag="PK362_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC73425.1"
                     /translation="MTPRRPPETEERPPSSALNALPTGTRTELWRALVRRRVKAGAAL
                     FQEGEPGEAAYVVMKGLFKALKFSQGERVSAMDLIVPGRLCGVIALLDHRPYPVSVYA
                     LQDAEVLVLPAAVFHRLMSQHPEFSRAVHGEVGDHLRHAQDMRARASEPVDKRLAHLL
                     LMLLPAPGAEVRVRREDLAELVGCIPETAIRTLSEFKKRGWIRTGWKRIALLNPDALR
                     RLSRSPANLP"
     gene            3255..3821
                     /locus_tag="PK362_00870"
     CDS             3255..3821
                     /locus_tag="PK362_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73426.1"
                     /translation="MRIGRGWVPFLLATTAVFGRSPGAPPRAPRMDFPADLGPATIDV
                     SSYPAEMQNIYRTNFLKNCAVCHSPARAIHSEFLELPTAEAAALRAASPTLFDDPRVL
                     LPGPDLWKRYIKRMKMRPPCCGVCPVMSETEARDIWRFLVFDGRIRKTGPRAGDWVRN
                     RRALLEDFRKRFPERYRDLFESPKEDSR"
     gene            3902..4819
                     /gene="nirK"
                     /locus_tag="PK362_00875"
                     /pseudo
     CDS             3902..4819
                     /gene="nirK"
                     /locus_tag="PK362_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
     gene            4946..>6019
                     /locus_tag="PK362_00880"
     CDS             4946..>6019
                     /locus_tag="PK362_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HNC73427.1"
                     /translation="MDAPRSTLGISPWWRHGAVLTVIFGLTGLIFMGRTTYRGAPPQF
                     NAVVEGSGTSLFTSEDVKAGQDVFQKYGLMDYGTLFGHGGYRGPDFTAETLHGLAEDL
                     REIYAAERGGSAYADLPESARASIASRVVTELKINRYDPATNNVTVTPAFATAFGRRV
                     DHLRTTLKDADRTRPLPTNYISNDEDLRTLAAFFTWSAWAAVTPRPGKNHTYTNNWPP
                     DETAGNRPSLAVVLWSALSLISFLGALGLVLMFFGRFNFLGWGGGKMDVSQVPPVDAI
                     PVTVGQKATFKYFLVVALLFLFQTLMGVVTAHYFVEGAGFYGIDTRSFLPLTITRSWH
                     LQLSIFWIATAWLATGMFVAPLLA"
BASE COUNT          975 a         2054 c         1851 g         1139 t
ORIGIN      
        1 cccctggagt ttgaatccct gtcccaatcc gcggccgaaa cccgccgccg ggccgaaggc
       61 cccaacgaac ttccctcttc ccaccgtcga gggtgggtcg tcctggccct ggacgttttc
      121 cgagagccca tgttcctcct tctggtggcc tgcggagccc tctatttcgc cctgggcgac
      181 gggcaggaag ccgcgatgct tttggggttc gtgttcttca tcatgggcat cacccttttt
      241 caggagggaa aaaccgaacg agccctggag gccctgcggg acctatcgag cccccgggcg
      301 ctcgtgattc gggacgggtc ccagcggcgc gtggccgggc gcgacgtcgt ccgcggcgac
      361 ctgctggtgt tggccgaggg agaccgtgtc ccggccgacg ggattcttct ctcctcctcc
      421 cacctggccg tggacgagtc cctgctgacg ggggaatccg tttcggttcg gaaagaggtc
      481 cgcgcggcga ttccgggcga aaccccgacc acgtcggggg acggatctcc ttacgtattc
      541 gccgggaccc tcgtggtcca ggggcagggc ctcgtgcgcg tcaccggcgt cggggccgcc
      601 acggaaatcg gaaaaatcgg ccgggccctg gaatccgccg agtcgggcct tagccccctg
      661 caaatcgaaa tggccgccct ggtgaaagtg gtcgccgtcg tgggagccat tctttgcgcg
      721 gcggtggttt tggtgaccgg tttttggcgg ggcgcctggc tgaccggcgt cctcgcgggc
      781 ctcagcctgg cgatggccct catgccgaac gaatttcccg ttgtcctcac gatttttctt
      841 tccctgggcg cctggcgact ttccaagcgg ggcgtcctgg cccggcgcat gcccgcggtg
      901 gaggccctgg gcgccgtcac ggttctctgc gtggacaaaa cgggcacctt gacggaaaac
      961 cgcatggaca ttcagcggct ccttcccggc gatcttcggt tcgacatttc gcccgcggga
     1021 aacaccctgg aggaggcgtt ccatgaaatc ctcgagttcg ggattttggc cagcgcccga
     1081 aaccctttcg atcccatgga aaaggccttt cacgccctgg gcgaccgatt cctcgcccgc
     1141 accgagcatc tccacgggga ctggacgttg gtcaaggaat accccctgtc ccgggaactg
     1201 ctggccatgt cccaggtgtg gacgtccccc gaccagaagc gcttcgtcat cgcggccaaa
     1261 ggcgcccccg aagccgtcgc ggacctctgc catttccccc ccgaccgcct cgaggccctc
     1321 cgccgaaagg tggacgaaca cgcccgggaa gggtaccgcc ttctgggagt ggccaaatcc
     1381 tctttcacgg cggaggtcct gccggacctc caacacgact tcgattttga attcctgggc
     1441 ttgatcgccc tggcggatcc ggtgcggcgg gacgtgcccg ccgccatcga ggaatgccgt
     1501 cgggcgggcg tccgggtcgc catgatcacg ggcgactacc cgggcaccgc ccagagcgtc
     1561 gcccgggcca tcggcttgtc caacgccgac cgggtgctca cgggacccga gatcgacacc
     1621 ctgtccgagg aggccctggc cgagcggctg cgccacgtga acgtcttcgc ccgggcggtg
     1681 ccggaacaca aactccgact cattcgggcc ctgcgggcca acggcgagat cgtggccatg
     1741 accggcgacg gggtcaacga cgcgccggcg cttaaagccg cccacatcgg cgtggccatg
     1801 ggcgaacgcg gaaccgacgt cgcccgggaa gccgcgggcc tggttctgct ccacgacgat
     1861 ttcgcctccc tggtggggtc catcgccggc gggcggcggg tgttcgacaa tttgaaaaag
     1921 ggcatggcct acatcctggc cattcacgtg ccgatcgccg ggctgtccct ggcgtcggtg
     1981 tttctggggt ggcccctggt cctcctgccg atccacattg ccttcctgca tttggtcatc
     2041 gacccggcct gttccgtggc ctttgaagcc gaaccgccgg agcccgacgc gatgcaacgc
     2101 cccccgcgga aaaccacgga ccgcttgttc ggatgggaca ccctgggccc gagcctggcc
     2161 cagggcgtga gcgttttcac cgcggtgttt gcggtgttcg ccctggctct tcgccggggc
     2221 caaggagagc tggacgcccg ggccctggct ttcaccacgc tcatggcggc caacgcggga
     2281 ctgatcctcg ccaaccgctc ctggacccaa tcgacctggg cccgctggcg ggcccccaat
     2341 cgcgccctct gggccgtggt cggaggctcg gcgctctttt tggccgtcgt gctttccacg
     2401 ccgcttttga ggcgcctatt tcatttttcg accctgcaca ccattgatat cgcggcgagc
     2461 gtggccgtcg gcacgttcag cgttttcctg gtggattgga ccgccaaacg gtgggcccgg
     2521 aggcggcctt gacgccccga cgccccccgg aaaccgaaga acgccccccg tcctcggcgc
     2581 tgaacgccct gccgaccggg acgcgaaccg agctttggcg agcgctcgtc cgacgacggg
     2641 tgaaggccgg ggccgcgctt ttccaggagg gggaacccgg cgaggccgcc tacgtggtga
     2701 tgaagggact gttcaaagcg ttgaaatttt cccagggcga gcgggtctcc gccatggacc
     2761 tgatcgttcc cggccgcctt tgcggcgtca ttgccttgct ggaccatcgg ccctaccccg
     2821 tgagcgtcta tgccctccag gacgccgagg tgctcgtgtt gccggcggcg gtctttcatc
     2881 ggctgatgtc ccagcatccg gaattttccc gggcggttca cggcgaggtg ggcgaccatc
     2941 tgcggcacgc ccaggacatg cgcgcccggg cctcggagcc cgtggacaaa cggctggccc
     3001 accttctgct gatgctcctt ccggcccccg gcgcggaggt gcgggttcgg cgcgaagact
     3061 tggccgaact cgtgggctgc attcccgaaa ccgccattcg caccttgtcc gaatttaaga
     3121 aacgcgggtg gatccgaacg gggtggaagc gaatcgctct cctcaacccc gacgccctgc
     3181 gccgcctgtc ccgctccccc gcaaacctgc cctaacgcat acttaagtcg gcctccaagc
     3241 tttaagcttt ccccgtgcgc attggacggg ggtgggttcc attccttttg gcgacgaccg
     3301 ccgttttcgg gcggtccccc ggcgcgcccc cgcgcgcccc gcggatggat ttccccgccg
     3361 atctgggccc ggccaccatc gacgtttcct cctacccggc cgaaatgcaa aacatttatc
     3421 ggacaaattt cctcaaaaac tgcgcggtct gccattcgcc ggcccgggcc atccattccg
     3481 aatttcttga acttccgacg gccgaagcgg cggcccttcg ggcggcgtcg cccaccctct
     3541 tcgacgaccc ccgggttctc cttccgggcc ccgatctatg gaaacgctac atcaagcgca
     3601 tgaaaatgcg ccctccctgc tgcggggtct gcccggttat gagcgaaacc gaggcccggg
     3661 acatctggcg gtttttggtt ttcgacggtc gaatccggaa aaccggcccc cgggccgggg
     3721 actgggttcg aaaccgtcgc gccctactcg aggatttccg gaaacgtttt ccggaacgct
     3781 atcgcgattt attcgaatca cccaaggagg attcccgatg acgcttcgac gaatcgtgct
     3841 gttggcggcc ggtctggccg gcgggggcct ggccgcattg gccgccccgg tcacggaaaa
     3901 agcggtgctc acccaggccc cccaggtacc ccccgccatc gcccggcggc atcccgccgt
     3961 ggttcaagtt cacctggaaa cacgggaaaa agaagggttg atcatgaacg ggtttgagga
     4021 gggcacccgt taccgctttt ggacgtttaa cgggcaggtc ccggggccca tgatccgcgt
     4081 gcgggaaggg gacaccattg agctgaccct caccaatccc aaagaaagca ccatggacca
     4141 caacatcgac ttgcacgccg tgacgggccc cggcggagga gccaagatca ccctggccaa
     4201 acccggggag gaaaaagtgg ctcgtttcaa agccttgaac ccggggcttt tcgtttacca
     4261 ctgcgccgcc ccgcccgtga cggaacatat cgccaacggg atgtacggga tgatacttgt
     4321 ggaacccgcc gggggcctgc ccaaagtgga ccgggagtat tacgtcatgc agagcgagtt
     4381 ctacaccaag gccgaattcg gggccgaagg gttgcaaagt ttcgatccgg acaaggccgc
     4441 cgccgaacgt ccgacctata ttgtcttcaa cggccgggtg ggcgcgcttc tgggcgaggg
     4501 ggccttgaaa gccaaaacca acgagaccgt gcgcgtctac ttcggcgacg gtggtcccaa
     4561 cctggtctcg agctggcaca tcatcgggga aattttcgac cgcctttggc ccgaaggggc
     4621 cttggcgtcg ccgcccctcc acagcgtcca aaccaccctc gtccccgcgg gcggatccgt
     4681 cgtggttgaa ttcaaaaccc aggtgcccgg cgattacacc ttggtggacc actcgatctt
     4741 tcgaatcgag aagggcgccc tgggatccct tcacgtcgac gggccggagg acccggcggt
     4801 ttatcaatcc gtcaaataat ccccgttttc tgacgctcat cgggccgggc ccttctcggg
     4861 gcccggcccg atgtctgttt tagcgcatgt tttgagcccc gctcttcttc tatcctgagg
     4921 gctcaactca aacctggagg cattcatgga cgcacctcga agcaccctcg gcatttcccc
     4981 ctggtggcgg cacggcgccg tgctcaccgt cattttcggc ttgaccggcc tgatattcat
     5041 gggccgcacg acgtatcgag gggctccccc ccaatttaac gcggtcgtcg agggttcggg
     5101 gacatccctt ttcacctccg aggacgtgaa ggcgggacag gacgtcttcc aaaaatacgg
     5161 cctcatggat tatggaacgc tctttggcca cggcggctac cgggggcccg acttcacggc
     5221 ggaaactctc catggactgg ccgaggacct gcgggagatc tacgccgccg aacgcggcgg
     5281 atcggcctac gccgacctgc ccgaatccgc ccgggcgtcc atcgcctcgc gcgtggtgac
     5341 ggagttaaaa atcaaccgct acgaccccgc gaccaacaac gtgacggtga cgcccgcctt
     5401 cgcgacggcc ttcggtcgac gggtggacca cctgcgaacc accctcaaag acgccgacag
     5461 aacccgcccg cttccgacca actacatttc caacgacgag gatcttcgga ctctggccgc
     5521 gtttttcacc tggtccgcct gggccgcggt gaccccccgc cccggcaaaa accacacgta
     5581 caccaacaac tggccccccg acgaaacggc gggcaatcgc ccctccctgg ccgtcgtgct
     5641 ttggagcgcc ctgagtctca tttccttcct gggcgctttg ggcctcgttc tgatgttctt
     5701 cggtcggttc aattttctgg gctggggcgg gggcaaaatg gacgtttccc aggttcctcc
     5761 ggtggacgcg atcccggtca ccgtgggcca aaaggccacc ttcaagtatt tcctggtcgt
     5821 ggcgcttctg tttctcttcc aaacgctgat gggcgtcgta accgcccact acttcgtgga
     5881 aggcgccgga ttttacggca tcgacacccg ctcctttttg ccgttgacca tcacccgaag
     5941 ctggcacctt caactttcga tcttttggat cgcgacggcc tggctcgcca cggggatgtt
     6001 cgtggcgccg ctgttggcc
//
LOCUS       DAOQLS010000025         6012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5687_length_6012_cov_3.983885, whole genome shotgun sequence.
ACCESSION   DAOQLS010000025 DAOQLS010000000
VERSION     DAOQLS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5687_length_6012_cov_3.983885"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..734
                     /locus_tag="PK362_00885"
     CDS             54..734
                     /locus_tag="PK362_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73428.1"
                     /translation="MNGPMEHIGGGELLSLALLAPRQVMGSLPVNIPTPPFIQMELAP
                     GMEAAHWDFRVLDEANQMIYRVEGDQIPKDLIVWDGQDKGLMKIRTGQVYTPLLILTG
                     KSGKIDRFYGEPVLFDVMQYTQGDIRRTEFLNESLFGTGSADIFPEMAPPFKSLLSQM
                     RRTVGTVYHVTVYYTPNELSLARARLKTLQSLLEQALMLDSTSFVFEAAPAVERGDIS
                     EIVFSAKL"
     gene            731..1582
                     /locus_tag="PK362_00890"
     CDS             731..1582
                     /locus_tag="PK362_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73429.1"
                     /translation="MIPFRPPFRWIFALALAAFFFGTGTVPARAADDVAQMMDSAMKL
                     YEQGNYKKAMRLFLNVINADPNNADAKEFRNRCMDKIVEGELGVRVDKPSKVPNLPGP
                     GLRAPGAAVTPGAPRSIRTFRPQFRPGPRVAGNAKGKGKKPVTTVAADKPTAKDILAQ
                     RDALVEDYRRKVLGKESPVWLDDKRNEIEVVLYMNRIFLPMTDQLTTDSYPMLEAAAA
                     EIRANPKKAVFLRAVDNMSPAVRHTMIDLPARRSAIVFSYFVHSTLGDKRPAEASPAL
                     TPDDLAD"
     gene            1616..2401
                     /locus_tag="PK362_00895"
     CDS             1616..2401
                     /locus_tag="PK362_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNC73430.1"
                     /translation="MNARHATLLLLLLGATPGPLRARLNTVEPWAPGFDTNLPIRRWN
                     HDVNVRVGRGARGTDLTELSYALSLPAGDNWEMGGQWGYTSLDGQSAQSDSGINDLTL
                     AAKYRWPATAVPKPAAAVAEFGLSLPTGDGDKGLGAGGFGFLAGGGFSYPVASITGYA
                     RGEIGVYTEGSGTRRGRTLSFTVGGERRIDAEWTATMDLRLVDHGRDKINGVRSLDKS
                     SEAYLVPGGFWSPPNAPLALQGALLLGLTRDAYDFGLLVGARF"
     gene            2506..3960
                     /locus_tag="PK362_00900"
     CDS             2506..3960
                     /locus_tag="PK362_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC73431.1"
                     /translation="MRQNKAPLFDTLLGHAKNRVVSFHTPGHKNGRGIDPRLRAFTGR
                     NMYYMDVTVFPEVDSLHDPVGPIKKAQQLMAMAYGVEHSFFLVNGSSVGNMIMLMSSC
                     RPGDSVIISRNAHKSTMAGVILSGVWPIWIQPKVDQNLDILFDSSPEQIEEALRLYPE
                     AKAVVVTSPTYNGVTADLVKIAEICHSRGKILLVDEAHGPHLKFHEELPISAVEAGAD
                     LCVQSTHKILSALSQGSVVHVQSDLVDITRVRRVVSLLQTTSPNYLVLASLDLARRQA
                     VLEGEKRLEKVIRAAEAGRKRLNTLKNIFCITRKDIQARGYDMDPTKLTINVTRTGLS
                     GHEIEDILAREYKVQVDAADLFNLIAILGIGSEKSDVDRLVDALADIDRKYRGEAQNW
                     VLPIPELTTEMVMNPRDVFLHHKAKRVPLSRSIGQISAQTLTPYPPGIPVLIPGERIT
                     PEIVNYLSDLAEKDIRVVGQEGDALRTVKVVAVR"
     gene            3994..4389
                     /locus_tag="PK362_00905"
     CDS             3994..4389
                     /locus_tag="PK362_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNC73432.1"
                     /translation="MAKKKTAAQEPPAKKRVKKQPGTQAYEKRLLQMREDIIHLVREN
                     QDNETENEVGDEADAATKSSERELLFELSDNERQSLDAVEAALRKINTGVYGLCESCR
                     TKIAVARLKAIPHARYCINCQSRFDSPRS"
     gene            4394..4633
                     /locus_tag="PK362_00910"
     CDS             4394..4633
                     /locus_tag="PK362_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNC73433.1"
                     /translation="MERPAPTAHTVDARGRFCPMPTLLAALTLEKMASGDVLELRADD
                     PATRRDLPAWCAESGHRFLGIDETPPAFVARIQKS"
     gene            4630..>6012
                     /locus_tag="PK362_00915"
     CDS             4630..>6012
                     /locus_tag="PK362_00915"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNC73434.1"
                     /translation="MTPRLRVESNAAGRPVVLGVFQTDGEPVWTRRAAAWPRSLLAGA
                     RDDGFTGNTGETHLIAAPGVRAAEKILLVGLGKKEAADGQSLRLFAAGAFRRLAGTRW
                     TTARIVVPDDVLPPADAAQAVAEGIGLAAYRFDKHRSKPSDDVLLKDIVLDVLPVDLR
                     AADDRVRIGSLWAEAVSCARDLINEPPSHKRPVVLGRWAQSLASGRIRVRVYGKRELE
                     KMGAGALLGVNRGSAHPPVLVHLHYAPKGKPRRRVAFVGKGITFDSGGLSLKPADGMM
                     TMKYDMAGAATVLSIFKILGRLAPDVEVHGVAPVTENMPGPDAYKPGDVVRALNGKTI
                     EVLNTDAEGRVVLADALTFATRLNVDEILDVATLTGAATVALGKNIAACLSNTPRMTE
                     RLRAAADAAGEKLWPLPLEATYREHLRSKVADLKNIGNPGEAGTIIGGLFLEEFVAGK
                     PWAHIDMAGVG"
BASE COUNT         1186 a         2016 c         1759 g         1051 t
ORIGIN      
        1 cccaagctgg tttcgtgtag cggtgcggcg ctggtcggca aaccgaccgc gttttgaacg
       61 gcccgatgga acacatcggc ggcggggaac tcctgtcctt ggcccttctg gcgccgcgcc
      121 aggtgatggg ctccctcccg gtgaacattc cgacgccccc gtttattcaa atggaattgg
      181 cgccgggcat ggaagccgcc cattgggatt ttcgcgtcct ggacgaagcc aaccaaatga
      241 tttatcgcgt ggagggcgac caaatcccca aggacttgat cgtctgggac ggccaggaca
      301 aaggattgat gaaaatccgc accgggcagg tctatacgcc cttgctcatt ttgaccggga
      361 aaagcggcaa aatcgaccga ttctacggcg aaccggtgct cttcgacgtc atgcaataca
      421 cccaggggga tatccgccgc acggagtttc taaacgaaag cctgttcggg accgggtcgg
      481 ccgacatttt tccggaaatg gcgcccccct tcaaatcgct tttaagccaa atgcggcgaa
      541 ccgtcggcac cgtttaccac gtgaccgttt attacacccc caacgaattg tctttggccc
      601 gggcgcggct caaaaccctt caatcccttc tggaacaggc gctcatgctc gattccacgt
      661 cgttcgtttt tgaagccgcc cccgcggtcg aacggggcga catcagcgaa atcgtttttt
      721 cggccaaact atgatcccct tccgcccgcc gttccgttgg atttttgccc tcgccttggc
      781 cgcgtttttc ttcggaacgg ggacggttcc ggcccgcgcg gccgacgacg tcgcccagat
      841 gatggacagc gccatgaaat tgtacgaaca agggaattac aaaaaggcca tgcgcctttt
      901 tttaaacgtc atcaacgcgg atcccaacaa cgccgacgcc aaggaattcc ggaaccgttg
      961 catggacaag atcgttgaag gcgagctggg ggtccgcgtc gacaaaccct ccaaagttcc
     1021 caatttgccg ggccccggcc tccgcgcgcc cggcgccgcc gtcacgccgg gcgcgccccg
     1081 gtccatccgc acctttcggc cccaatttcg tcccggcccc cgggtcgccg ggaacgccaa
     1141 aggcaagggg aaaaaaccgg tcaccaccgt ggcggccgac aaacccacgg ccaaggatat
     1201 tctggcccaa cgggacgccc tggtggaaga ctaccggcgg aaagttctcg gcaaagaaag
     1261 ccccgtgtgg ctggacgata aacggaacga aatcgaagtc gtcctctaca tgaaccgaat
     1321 tttccttccc atgaccgatc aattgaccac ggactcctac cccatgctcg aagcggcggc
     1381 cgccgaaatc cgcgccaacc ccaaaaaagc cgtgtttctc cgggccgtcg acaacatgtc
     1441 gccggccgtc cgccacacca tgatcgacct cccggcgcgg cggagcgcca ttgtcttcag
     1501 ctatttcgtc cattccaccc tgggcgacaa acgccccgcc gaagcctcac cggccctcac
     1561 gccggacgac ctggccgatt aggccaaaaa taaaaaccac ccaaaggaac gaccgatgaa
     1621 cgcacgccac gccacactgc ttttactttt attgggagcg acgcccggac ccctgcgagc
     1681 ccggctgaac accgtggaac cttgggcccc cggttttgac acgaaccttc cgatccggcg
     1741 atggaaccac gacgtgaacg ttcgcgtggg tcgcggggcc cgggggacgg atctcaccga
     1801 attgtcctac gccctcagcc tccccgccgg ggacaattgg gaaatgggcg gccaatgggg
     1861 gtacacttcc ctggatggcc agagcgccca aagcgattcc ggaatcaacg atttgaccct
     1921 ggcggccaaa tatcggtggc ccgcgaccgc cgtgccgaaa ccggcggccg ccgtcgcgga
     1981 attcggtctg tcgctcccca ccggggacgg ggacaaaggt ttgggcgccg gcgggttcgg
     2041 ctttctggcc ggcggggggt tcagttaccc cgtcgcgtcc atcaccggct acgcccgggg
     2101 cgaaatcggg gtttacaccg aggggtcggg cacccgccgc ggccgcaccc tctccttcac
     2161 ggtgggcggc gaacgccgca tcgacgcgga atggacggcg acgatggacc ttcgcctggt
     2221 ggaccacgga cgggacaaaa tcaacggcgt ccgatccctg gataaatcgt ccgaggccta
     2281 cctcgttccc ggcgggtttt ggtccccgcc caacgccccg ctggcgctcc agggggccct
     2341 cttgctcggt ctcacgcggg acgcctacga tttcggcctt ctggtcggcg cccgtttttg
     2401 atcgcccgcc gtcgatcgct ccccgttctt tccgtcggat attttcttga caagggggga
     2461 gaaatgccct taaatgtggc accggcctag accggtctca accccatgag gcaaaacaaa
     2521 gccccgctct tcgacacgtt gctgggccac gcgaaaaacc gcgtggtctc ttttcacacg
     2581 cccggccata aaaacggccg gggcatcgac ccccgcctcc gcgccttcac cggccggaac
     2641 atgtattaca tggacgtcac cgttttcccg gaggtggatt cgctccacga ccccgtcggt
     2701 cccatcaaaa aggcccagca gttgatggcc atggcctacg gggtggaaca ctccttcttc
     2761 ctggtcaacg gttcctccgt cggcaacatg atcatgctga tgtcctcctg ccggccgggc
     2821 gactcggtca tcatttcgcg caacgcccac aaatcgacca tggccggagt gatcctgtcc
     2881 ggcgtctggc cgatttggat tcaaccgaag gtcgatcaaa acctcgacat ccttttcgat
     2941 tcgagccccg aacaaatcga ggaggccctt cggctctatc ccgaggccaa ggcggtcgtc
     3001 gtcaccagcc ccacctacaa cggcgtgacc gccgatctcg tgaagatcgc cgagatttgc
     3061 cactcccggg gaaaaatcct tttggtcgac gaagcccacg ggccccatct taaatttcac
     3121 gaggaattgc cgatttcggc ggtggaagcc ggggccgacc tgtgcgtcca atccacccac
     3181 aaaatcctct cggccttgtc ccagggatcc gtggtccacg ttcaatcgga cctcgtggac
     3241 atcactcggg ttcggcgcgt cgtgtcgctc ctgcaaacga cgagccccaa ttacctcgtt
     3301 ttggccagtt tggacttggc gcgccggcag gccgtgctgg aaggcgaaaa acggctggaa
     3361 aaagtgattc gggccgccga agcgggccga aagcgactca acaccttgaa gaacatattc
     3421 tgcatcaccc gcaaagacat tcaggcccgc ggctacgaca tggatccgac gaagctcacg
     3481 atcaacgtca cccgcacggg gctctccggc cacgagatcg aggacatttt ggcgcgggaa
     3541 tacaaggtgc aggtcgacgc cgccgactta ttcaatttga tcgccatcct gggcatcggg
     3601 tccgagaaat ccgacgtgga ccgattggtg gacgccctgg ccgacatcga ccgcaaatac
     3661 cggggcgaag cccagaactg ggtgttgccc atcccggagc tcaccaccga aatggtgatg
     3721 aatccccggg acgtgttcct tcaccacaag gcgaagcgcg ttcccctgtc ccgctccatc
     3781 ggccaaattt ccgcgcaaac cctcacgccg tacccgcccg gcattccggt gttgatcccg
     3841 ggcgaacgaa tcactccgga aattgtaaac tacctttccg atttggccga gaaagacatt
     3901 cgcgtcgtcg gccaagaagg cgacgccctt cggaccgtga aagtggtggc cgtccgctga
     3961 cctcggcggc gccttccaga aagagagacg accatggcga agaaaaaaac ggcggctcag
     4021 gagcctccgg cgaaaaagcg agtcaagaaa caacccggca cgcaggccta cgaaaaacgc
     4081 ctgttgcaaa tgcgggagga catcatacat ttggtccggg aaaaccagga caacgaaacc
     4141 gaaaacgagg tgggcgacga agccgacgcc gccaccaagt cctccgagcg cgaactgctt
     4201 ttcgaattgt cggacaacga gcgccagtcc ctggacgccg tcgaggcggc cctccgcaag
     4261 atcaacacgg gcgtctacgg tctgtgcgag tcctgtcgga cgaaaatcgc cgtggcgcga
     4321 ctcaaggcca tcccccacgc gcgctactgc atcaactgcc aatcccgttt cgactccccg
     4381 cggagctgag gccgtggaac gtcccgcgcc cacggcgcac accgtggacg cccggggtcg
     4441 cttttgcccc atgcccacgt tgttggccgc tctgacactc gagaagatgg cctccggcga
     4501 cgtgttggaa ttgcgcgccg acgatcccgc cacccgccgg gatctccccg cctggtgcgc
     4561 cgaatccggc catcgtttcc tgggaatcga cgaaacgccc cccgctttcg tggcccgcat
     4621 ccaaaaatca tgaccccccg ccttcgcgtc gaatccaacg ccgccggacg gcccgtcgtt
     4681 ctgggggttt tccaaaccga cggcgaaccc gtttggacgc ggcgcgccgc ggcctggccc
     4741 cggtccctcc tggccggcgc ccgggacgac ggattcacgg gcaatacggg cgaaacccat
     4801 ttgatcgccg ctccgggggt tcgcgcggcg gaaaaaattc ttctggtcgg gctcgggaaa
     4861 aaggaagccg cggacggcca gtccctgcgg cttttcgcgg ccggcgcttt tcggcgtttg
     4921 gcgggcaccc ggtggaccac ggcgcggatc gttgttcccg acgatgtcct tcccccggcc
     4981 gacgccgccc aggcggtggc cgaaggcatc ggattggcgg cctaccgttt cgacaaacac
     5041 cggtccaaac cttccgacga cgtccttttg aaggacatcg ttttggacgt cctcccggtc
     5101 gaccttcggg ccgccgacga ccgcgtccgg atcgggtcct tgtgggccga agcggtgtcc
     5161 tgcgctcggg acctcatcaa cgaacccccg agccacaaac ggccggtcgt cctcgggcgc
     5221 tgggcccaat cgttggcgag cgggcggatt cgcgtgcgtg tttacggcaa gcgggaactg
     5281 gaaaagatgg gcgccggagc cctcttgggg gtcaatcgcg gcagcgccca tccgccggtt
     5341 ctcgtccact tgcactacgc ccccaaggga aaaccccggc gtcgcgtcgc ttttgtcggc
     5401 aaggggatca ccttcgactc cgggggattg tccctcaagc ccgccgacgg catgatgacc
     5461 atgaaatacg acatggcggg ggccgccacg gtcctttcca ttttcaaaat tctgggacgg
     5521 ctcgcccccg acgtggaagt ccacggggtg gcccccgtca ccgaaaacat gccgggcccc
     5581 gacgcctaca aaccgggcga cgtcgtgcgg gctctgaacg gcaaaaccat cgaagtcctc
     5641 aacacggacg ccgaaggccg cgtggtgttg gccgacgcgc tcacctttgc gacgcggttg
     5701 aacgtggacg aaattctgga cgtggcgacg ctcaccggcg ccgccacggt ggccctggga
     5761 aaaaacatcg ccgcctgcct gtccaacacc ccgcgaatga cggagcgcct ccgggccgcg
     5821 gccgacgcgg cgggggagaa actctggccg ttgccgctgg aggcgaccta ccgcgagcat
     5881 cttcgcagca aagtggccga ccttaaaaac atcgggaacc cgggggaagc cggcaccatc
     5941 atcgggggcc ttttcctcga ggaattcgtt gccggaaaac cctgggccca catcgacatg
     6001 gcgggggtgg gg
//
LOCUS       DAOQLS010000026         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5800_length_5950_cov_4.807803, whole genome shotgun sequence.
ACCESSION   DAOQLS010000026 DAOQLS010000000
VERSION     DAOQLS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5800_length_5950_cov_4.807803"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2684)
                     /gene="uvrA"
                     /locus_tag="PK362_00920"
     CDS             complement(<1..2684)
                     /gene="uvrA"
                     /locus_tag="PK362_00920"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNC73435.1"
                     /translation="MSDDVIKIRGAREHNLKNLTLEIPRNKMVVVTGLSGSGKSSLAF
                     NTIYAEGQRRYVESLSAYARQFLELMEKPDVDLIEGLSPAIAIEQRTPSHNPRSTVGT
                     VTEIYDYLRLLYARVGTPHCPDCGREIEPRSATQIIGDILKEFDGQNVQILAPLVRGR
                     TGTYEALFDKLKKSGFARVRVNNELRDLEKKIALDRYKKHTIEAVVDRLAVAAAGRTR
                     LADSVETALRESRGLLVVAPADGKGPDRLFSEHHACPHCGTSLPEIEPRLFSFNSPYG
                     ACANCDGLGVKLEVAEDLVVTDESLSIADGALSAWADPVTTRTNRWKKSWSGYYMEIL
                     EEVCRRNKIPLDKPWRSLLPAQRKSLLHGGMDHKSPWGKNVKEFEGVIGNLERRYKES
                     ESAFVKEEIQARFMRSRLCPDCKGARLKPEALAVTVGGKSIAQATRLSVQEAHAFFNA
                     LPLSEKSQFVARQVLKEIRSRLAFLVDVGLEYVNLDRESATLAGGEAQRIHLATQIGS
                     GLVGVLYVLDEPTIGLHPRDNARLLTTLNRLRDIGNTLVIVEHDEETIRAADWVIDLG
                     PGAGVHGGQIIAQGTVKDLLKESKSLTGAYLRGERGIPVPAERRRPGEKFLEIKGASQ
                     FNLKSIDVKIPLGLFVAVTGVSGSGKSTLVNEILHKALAQKLHHAKDEPGRHRTLAGV
                     ENVDKVVDVDQTPIGRTPRSNAATYTGAFGPIRDLFAQLPEARRRGYKPGRFSFNVKG
                     GRCENCEGDGTLKISMQFLPDVYVKCDVCAGQRFNAETLEIKYKGKSIAEVLAMPAEE
                     AAVFFENIPAVARVLQTLVDVGMGYAAIGQPATTLSGGEAQRVKLASELCRRPTGHTL
                     YILDEPTTGLHFADVEKLLGVLQRLVDGGNTVLVIEHNL"
     gene            complement(2681..4342)
                     /gene="nadB"
                     /locus_tag="PK362_00925"
     CDS             complement(2681..4342)
                     /gene="nadB"
                     /locus_tag="PK362_00925"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNC73436.1"
                     /translation="MSKPFQSDFAIVGSGIAGLSLALKAAEHGTVHLVTKRAIQDSAT
                     DYAQGGLAAVISEKDSFDDHVKDTLSAGAGLCDPAVVRRVVTEAPDRLKDLVRWGVRF
                     SSAPSLDPNEQRFELGLEGGHSQRRILHVGDYTGHAVEQVLVDRVRAHPRIQVFEHHF
                     AVDLITRRRLGLRKGPDACAGAYVLDVDSGVVHTFVAKATALCTGGAGKVYLYTSNPD
                     VATGDGMAMAYRAGAVLANMEFVQFHPTCLYHPKAKNFLISEALRGEGAVLKNRAGER
                     FVKKYDRRGELAPRDAVARAIDAELKRTGDECVFLDISHRGAEYVRRRFPKIYERCLQ
                     FGIDITAQPIPVVPAAHYFCGGVRTDVRGRTSLSRLSAVGEVACTGLHGANRLASNSL
                     PEAMVFADAVAADWKLLADGNFVVPPARVPRWNPGKARNPDELVVISQVWEEIRRFMW
                     NYVGIARTNKRLERARRRVEMLQREIQEQYWDFLLTPDLLELRNIAAVAELVIRGALF
                     RRESRGLHYTADYPRTLKTARGTLLARGADGRPRAGAGSPVRGGS"
     gene            complement(4412..5413)
                     /locus_tag="PK362_00930"
     CDS             complement(4412..5413)
                     /locus_tag="PK362_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73437.1"
                     /translation="MRSTLYSLAEATAKIKTGAPLLLAGDESLLKKLPPGPWIGGTIP
                     YFMTVESGGVFSRDRVFITELPPSTENVRIVVLEDRHLREIYQQMPDNGFGFIIMPGS
                     SKTQLHFALNAPRYPAFGVRPLVGWVSGIDLAESGKTKPKVVDGQSGRIFEEGAVVMY
                     VSLPKTKVANVGIHNIFSSGTGDDLTFEAEGFSAGEVLVNGRKQNFGRYLKENKINTK
                     LPLVTNLNGVNLNVAFAGVDEKTGVASFYAPVFKGLTYRHAVLEGDYVDRFLSDFPVD
                     LGGSVCFSCNCICNYLHSNLQGRKMGALAGPITFGEVAYQLLNQTMVYLSITDAVKI"
     gene            complement(5424..5693)
                     /locus_tag="PK362_00935"
     CDS             complement(5424..5693)
                     /locus_tag="PK362_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73438.1"
                     /translation="MIQEKGGAFPVRDIPGATHPRWRSLVTGEIHLPLEFLATKILLI
                     RAKMETTLNKAPEVVPALCQEVYELHAKNLTNPKVQSDLRLIFHP"
BASE COUNT         1094 a         1742 c         1894 g         1220 t
ORIGIN      
        1 agattgtgtt cgatgaccag caccgtgttg ccgccgtcca ccaggcgctg aagcacgccc
       61 aacagtttct ccacgtccgc gaagtggagg cccgtggtgg gttcgtccaa aatatagagc
      121 gtgtgtcccg tggggcggcg gcacaattcc gaggccagct tcacccgttg ggcttcgccg
      181 ccggacaaag tcgtggcggg ttgaccgatg gcggcgtagc ccatgcccac gtccaccagg
      241 gtttgcagca cgcgggcgac ggcgggaatg ttttcaaaga acaccgccgc ttcctcggcg
      301 ggcatggcca gaacctcggc gatggatttg cctttgtatt tgatttcgag ggtttcggcg
      361 ttgaaccgtt gtccggcgca gacgtcgcat ttcacgtaaa cgtccggcag aaattgcatc
      421 gatattttca gggtgccgtc cccttcgcaa ttttcgcaac gcccgccctt cacgttgaac
      481 gagaaacgtc cgggtttgta tccccggcgc cgggcttccg gcaattgggc gaagaggtcc
      541 cgaatgggac caaacgcccc ggtgtaggtc gccgcgttgg accggggcgt gcggccgatg
      601 ggcgtttggt ccacgtccac cactttgtcc acgttttcca cgccggccaa ggtccgatgg
      661 cggcccggct cgtccttcgc gtggtggagc ttctgggcca gggctttgtg gaggatttca
      721 ttcaccaggg tggatttgcc ggaacccgaa acgccggtga ccgccacgaa gagccccaag
      781 ggaattttga catcgatgct ctttagattg aattgggagg cgcctttgat ctccagaaat
      841 ttttccccgg gccgtcgtcg ttccgcgggc acgggaattc cgcgttcccc gcgcaaatag
      901 gccccggtca acgatttcga ttcctttagc aggtctttca cggttccttg ggcgataatt
      961 tggccgccgt gaacgcccgc cccgggaccg aggtcgatga cccaatcggc ggcgcggatg
     1021 gtttcctcat cgtgctccac gatcaccagg gtattgccga tgtcccgcaa gcggttgagg
     1081 gtggttaaaa gtcgggcgtt gtcccggggg tggaggccga tggtgggttc gtccaacaca
     1141 tacaaaacgc ccaccaggcc ggaaccgatc tgggtggcca ggtggattcg ttgggcctcg
     1201 cccccggcca gggtggccga ttcccggtcc aaattgacgt attccaatcc cacgtccacc
     1261 agaaacgcca accgggagcg gatttctttg agcacctgcc gagcgacgaa ttgggatttt
     1321 tcggaaagcg gcagcgcgtt gaaaaaagcg tgggcttcct ggaccgaaag ccgcgtggct
     1381 tgggcgatgg atttcccccc cacggtgacg gccagggcct cgggtttgag gcgcgcccct
     1441 ttgcagtccg ggcagaggcg gctgcgcatg aatcgcgcct gaatttcttc cttcacgaag
     1501 gcggattcgg attccttgta gcgacgttcc aggttgccga tgacgccctc gaattccttg
     1561 acgttctttc cccaggggga tttgtggtcc atgccgccgt ggagcaggga tttccgttgg
     1621 gccgggagca gggaccgcca aggtttgtcc agggggattt tattccgccg gcagacctcc
     1681 tccaggattt ccatgtaata acccgaccag gattttttcc aacggttcgt ccgggtggtg
     1741 accgggtcgg cccaggccga cagggccccg tcggcgatgg acagcgactc gtcggtgacg
     1801 accaaatctt cggccacttc cagtttgacc cccaggccgt cgcagttggc gcaggcgccg
     1861 taggggctgt tgaaggaaaa aaggcggggt tcgatttcgg gcaggctcgt gccgcaatgg
     1921 gggcaggcgt ggtgctcgga gaaaagccgg tcgggccctt tgccgtcggc cggggccacg
     1981 accaggagtc cccgggactc ccgcagggcg gtttccacgg aatccgccag ccgggttcgc
     2041 cccgcggcgg cgaccgccag gcgatccacc acggcctcga tggtgtgctt tttgtagcgg
     2101 tccagggcga tctttttttc caaatcccgg agctcgttgt tcacccgaac ccgggcgaaa
     2161 ccggattttt ttaatttgtc gaaaagcgcc tcgtaagtgc cggtccgtcc acggaccagg
     2221 ggggccagga tttgaacgtt ttgaccgtcg aattccttta ggatgtcccc gatgatttgg
     2281 gtggcggagc gcggttcgat ttcccgtccg cagtcggggc aatggggcgt tcccacccgg
     2341 gcgtagagca ggcggaggta gtcgtaaatt tcggtcacgg ttcccacggt ggaccgcggg
     2401 ttgtgggacg gggtccgttg ctcgatggcg atggcggggg aaagcccttc gatcaaatcg
     2461 acgtcgggct tttccatgag ctccaggaat tgacgggcgt aggccgacag gctctcgacg
     2521 taacgccgct gtccttcggc gtaaatggtg ttgaacgcca gggagctttt gcccgacccc
     2581 gaaagccccg tcaccaccac cattttgttt cgggggattt ccaaggtgag gtttttaaga
     2641 ttgtgttccc gggcgccccg gattttgatg acgtcgtcgc tcacgatccg cctctcaccg
     2701 gcgagccggc cccggcccgg gggcgtccgt ccgccccccg cgccaacaag gtgccccggg
     2761 ccgttttaag cgttcgggga taatccgccg tgtaatgaag gccccgggat tcgcgccgga
     2821 acaaagcgcc ccgaatgacc aattccgcca cggcggcgat gtttcgaagt tccaggagat
     2881 ccggcgtgag caggaaatcc cagtactgct cctggatttc ccgctggagc atttccaccc
     2941 ggcggcgggc ccgctccaac cgcttgttgg ttcgcgcgat gcccacgtaa ttccacatga
     3001 agcggcggat ttcttcccac acctggctga tgacgaccaa ttcatcgggg tttcgggctt
     3061 tgcccgggtt ccaccggggc acccgggccg gggggaccac gaaattgccg tcggccagga
     3121 gtttccaatc ggcggccacg gcgtcggcga agaccatggc ttcgggcagg gaattggagg
     3181 ccaggcggtt ggcgccgtgc agaccggtgc aggccacttc ccccacggcc gacagccggg
     3241 acaaggatgt tcgtccacgg acgtcggtcc gcacgccccc gcaaaaatag tgagccgccg
     3301 gcacgaccgg aatgggctgg gccgtgatgt cgatcccgaa ttggaggcaa cgctcgtaaa
     3361 tttttgggaa gcgacgccgg acgtattcgg cgccccggtg ggaaatatcc aaaaaaacgc
     3421 actcgtcgcc cgtccgcttc agttcggcgt caatggcccg cgccacggcg tcccgggggg
     3481 cgagctcccc ccggcggtcg tatttcttga cgaaccgttc tccggcgcgg tttttaagaa
     3541 cggccccttc tccccggagg gcttcggaga tcaaaaagtt tttggccttg gggtggtaca
     3601 ggcaggtggg gtggaattgc acgaattcca tgttggccaa gacggccccg gcccggtacg
     3661 ccatggccat gccgtcgccc gtggccacgt cggggttcga ggtgtaaaga tacaccttgc
     3721 ccgcgccgcc ggtgcagagg gccgtggctt tggcgacgaa ggtgtggacg acgcccgaat
     3781 ccacgtccaa cacgtaggcg ccggcgcagg cgtcggggcc ctttcgaagg cccaaccgtc
     3841 ggcgggtgat aaggtccacc gcgaaatggt gttcaaagac ctggatcctt ggatgggctc
     3901 ggacccgatc caccaggacc tgctccacgg cgtggccggt gtaatccccg acgtgcagga
     3961 tgcggcgttg ggagtggccc ccctccaggc ccagctcgaa tcgctgttcg ttggggtcca
     4021 agctgggggc ggaggaaaaa cggacgcccc agcgcaccaa atcttttagc cggtccgggg
     4081 cctcggtgac cacccggcgc acgacggcgg gatcgcagag cccggccccc gcggacaggg
     4141 tgtctttgac gtgatcgtcg aaggaatcct tttcggaaat caccgccgcc aggccgccct
     4201 gggcgtagtc cgtggccgaa tcctgaatgg cccgcttggt caccaaatga acggtgccgt
     4261 gttccgccgc cttcaaggcc agggacaatc ccgcgatccc cgatccaacg atggcgaaat
     4321 ccgattgaaa gggttttgac atggagggag attgtaccag ttgaccgccc ggcgggagcg
     4381 acgaaaaagg gcccccggga gctcgatgga attatatttt gacggcgtcc gtgatggaaa
     4441 ggtagaccat ggtttggttt aacaattgat acgccacttc cccgaaggtg atgggaccgg
     4501 ccaacgcccc cattttacgg ccctgaagat ttgagtgcag gtaattgcaa atgcaattgc
     4561 aggagaagca aaccgacccg cccagatcga cgggaaaatc ggagaggaac cgatccacgt
     4621 aatccccttc caggacggcg tgtcggtagg tcagaccttt gaacaccggg gcgtaaaagg
     4681 acgccacccc cgttttttcg tccacccccg caaaggcgac gttgaggttc acgccgttca
     4741 aattggtgac caggggcagt ttggtgttga ttttattttc tttaagataa cggccgaaat
     4801 tttgttttcg gccattgacg agcacttccc cggccgagaa gccttcggcc tcaaaggtca
     4861 aatcgtcgcc cgtgcccgaa ctgaaaatat tgtgaatgcc gacgttggcc accttggttt
     4921 tgggcaagga cacatacatg accaccgccc cttcttcgaa aatccttccc gattgtccgt
     4981 ccacgacttt gggtttggtt tttcctgatt ccgccaaatc gattcccgac acccagccga
     5041 ccagcggccg gacgccaaag gccgggtagc ggggggcgtt caaggcgaaa tggagctgtg
     5101 ttttggaaga tcccggcatg atgataaagc caaaaccgtt gtcgggcatt tgttgataaa
     5161 tctcccggag gtggcggtct tccagaacca caattcggac gttctcggtg gaggggggca
     5221 attcggtgat aaacacccgg tctcgggaaa agacgccccc gctttccacc gtcatgaaat
     5281 aagggatggt tcctccgatc cagggaccgg ggggaagttt tttcaacaga gactcgtctc
     5341 ccgccaaaag gagcggcgcg ccggttttga tttttgccgt cgcttcggcc aacgaataaa
     5401 gcgtggagcg catggcggac tccttaggga tggaaaatca aacgaagatc gctttgaact
     5461 ttcggattgg tcaaattctt cgcgtgcaat tcataaactt cctggcacaa cgcgggaacg
     5521 acctccggcg ctttgttgag ggtcgtttcc attttcgcgc ggatgagaag tatttttgtg
     5581 gcgagaaatt ccaacgggag atgtatttcg cccgtcacga gggagcgcca ccgggggtgg
     5641 gtggccccgg ggatgtcccg gactggaaag gccccgcctt tttcctggat catggtggat
     5701 ctaagcttat cccctccggg gcccgctttc aatggttttc ccggttcttt ccgtcggttt
     5761 ggcaaactca ggtcgtggac gattcccgga ggggatggcg gtgaaaacac cgggggcgac
     5821 cgggcccctc ggtgatcagc gaagggcctt gaacagtcgc agggcgtgcg ccgtgtcttt
     5881 gacgttgtga acccggagga cggcggcgcc ttcctgggcg gcgcggaggt tggcggccag
     5941 ggatccggat
//
LOCUS       DAOQLS010000027         5931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5836_length_5931_cov_4.952519, whole genome shotgun sequence.
ACCESSION   DAOQLS010000027 DAOQLS010000000
VERSION     DAOQLS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5836_length_5931_cov_4.952519"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1204
                     /locus_tag="PK362_00940"
     CDS             77..1204
                     /locus_tag="PK362_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC73439.1"
                     /translation="MKSKWWILGLALPWVAACQRGPREIKIALALPLTGDIASVGQGI
                     QRSATLALEEATTAGRFKSPVVLVPFDDRSDPKEAVNVANRVVSDPDTVAVIGHFNSG
                     CSIPASRVYAQAGLPMVSPGSSNPELTRQQLSANWPWPKNVFRANTTDDAQGTFAAEF
                     LNRTLKKKTVAVVHDKTSYGQGIAEEMQKRFAALGGVVVSFDGVQTGDKDFKALMTRI
                     KAENPAALFYGGTYTEGALILRQARDAGIGALFLTGENSFDPEFVRMAGPAAEGAYVT
                     YLGRPPELLDTAKAFVDKYKLRYPGQEIKAYDHYGYEITNMLLEALEKVGPDKAKIIE
                     YLRGLRYTGVLGETTFDEKGDTRNRTITLFVIKKGVFTRSL"
     gene            1167..1721
                     /locus_tag="PK362_00945"
     CDS             1167..1721
                     /locus_tag="PK362_00945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC73440.1"
                     /translation="MSSKKAFSPGPFSVVLAALLLAACAGPFARSRFHPAAGRGPSDP
                     PLKIHFRLPLERARILSPFGTRHGRLHTGIDLLETRGGGDPVLAAADGRVVGIGRGGP
                     YGRSILLEHAGGYRTRYAHLRKSLARPNAHVAAGEKIGIVGRTGNATTAHLHFEVITP
                     SGRFLDPGPLLFPPSPRPAGKSKK"
     gene            1754..2638
                     /locus_tag="PK362_00950"
     CDS             1754..2638
                     /locus_tag="PK362_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNC73441.1"
                     /translation="MAEQLLNGLTLGGIYALIALGYTMVYGVLQMINFAHSELFMWGG
                     VAGALFLSFLPADAGGGALIAAFVVAMGASGILAMILDRVAYAPVRRSSRLTPLISAI
                     GASLFLQNLVFLWRDSQMAFPQPLAVRSFPCGPVTVNGLQVFIVGGSVALMGALTFFV
                     RRTKMGKAMRAVAQDADTAELMGIPVNRVIALTFFIGGALGGAAGVMNGLYYGSVKYN
                     MGFLPGVKAFTAAVLGGIGNLPGAMLGGFLLGVVEALGTGFLPEPEWKDVFAFAVLIL
                     VLLFRPSGLLGENTAEKV"
     gene            2664..3617
                     /locus_tag="PK362_00955"
     CDS             2664..3617
                     /locus_tag="PK362_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73442.1"
                     /translation="MAAFLAAPLVLQGTSWAFVIRILALMGLYGLLALGVNLVLGYVG
                     LLDLGFMAFYAIGAYTTALLSLRGVGFWWCLPASVLAAVTVRTLLGWPVLRLRGDYLS
                     IVTLGFGEITRIALNNWDSLTNGPKGLSLLSSAAVPPLRFFGFGIQTNTHFFYLIFAL
                     LSLGLLVCARLNRSRIGRAWMAIRDDELAARAMGVNAPRLKNLAFGLSAGFAGVAGAI
                     FARWENFVTPESFTFWESALLVAMVVLGGMGSLPGVLVGVALILGLPEILRSGVFRWI
                     GGAQAVNARYLVFGAILVAMAIFRPQGLWPRRVDRLPAGNE"
     gene            3618..4397
                     /locus_tag="PK362_00960"
     CDS             3618..4397
                     /locus_tag="PK362_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73443.1"
                     /translation="MRLLGVRGVSKKFGGVAALDGVEFDIESGEIVSVIGPNGAGKTT
                     LFNVLTGFYRPDAGAIEFSGERIDDLPSHRIAKRGIARTFQNIRLFGRMTALENVMVG
                     RHCRTRSELWPALLRTRSFYREEREIRHSALERLKAVGLEKEAHALARNLPYGDQRRL
                     EIARALAAEPRLLLLDEPNAGMNPQESEGLMALIRRIRGEGVTVLLIEHAMRVVMGVS
                     DRVIVLDYGKKIAEGPPAAVQSDPRVIEAYLGAPREEDARA"
     gene            4390..5097
                     /locus_tag="PK362_00965"
     CDS             4390..5097
                     /locus_tag="PK362_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73444.1"
                     /translation="MLEVKNVSASYGRKLRALHGVSLTVGRGEIVCLIGANGAGKSTL
                     LKTLSGLIWPDAGEILFDGRRVDSLPAHATAERGLIHVPEGRRIFPRMTVRENLEMGA
                     YTGAAAEARRGIDRVVRLFPVLGDRLSQWGGTLSGGEQQMLAMGRALMARPRLLMLDE
                     PSMGLAPLVVQKIFEIIRDINRDGTPILLVEQNANEALRVAHRGYVIETGRIAHEGSA
                     RDLRTDPKVRNAYLGVS"
     gene            5133..5903
                     /locus_tag="PK362_00970"
     CDS             5133..5903
                     /locus_tag="PK362_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNC73445.1"
                     /translation="MELVCVGTELLTGKISTHGAAFGALLEDRGWPLTRETTVADDPR
                     EMEAVFREIWRRSDVTIVTGGLGPTFDDVTREVWSKVTGRRLRRNRAVEAVIAGRFRA
                     RGLRMPPANRRQGMILDGATVLTNDAGTAPGQCLWTGKKMLVLLPGPPRELTPMVENH
                     WLPLFRRHFPDGRRATRVWRFFGLSESAVDEVLRSLVRRPGEVWGILAQSFLVDAKLT
                     LADVDPARAARRFAEVEKSLRRAFGAALFGTGRETMEE"
BASE COUNT          941 a         1914 c         1928 g         1148 t
ORIGIN      
        1 acgttgatgg tcgcgctcac caccgcggcg gcgtgccagc tgcgcgtgcg cgaccatcaa
       61 cgtccggggg cttaagatga aatcgaaatg gtggattttg ggattggctt tgccgtgggt
      121 ggccgcgtgc cagcgcgggc cccgggaaat caaaatcgct ttggctctgc ccctgaccgg
      181 cgacatcgcc tcggtgggcc agggcattca acggtcggcg accctcgccc tggaggaggc
      241 caccaccgcc ggacggttta aatcccccgt cgttttggtt cccttcgacg accggtccga
      301 tcccaaagag gccgtgaacg tggccaaccg cgtcgtctcg gacccggaca ccgtggcggt
      361 catcggccat ttcaattcgg ggtgctccat cccggcctcc cgggtgtacg cccaggcggg
      421 acttccgatg gtcagcccgg gctcctccaa cccggaattg acccgccaac agctctcggc
      481 caactggccc tggccgaaaa acgttttccg ggccaatacc accgacgacg ctcagggcac
      541 cttcgccgcg gaatttctaa accggacgct caagaaaaag accgtggccg tcgtgcacga
      601 caaaacgtcc tacggccagg gcatcgccga ggagatgcaa aagcggttcg cggcgctggg
      661 gggcgtcgtg gtctccttcg acggcgtcca gaccggcgac aaggatttca aagccctcat
      721 gacgcggatc aaagccgaaa accccgcggc cctgttttac ggcggcacct acaccgaagg
      781 cgcgctcatc ctccgacagg cccgggacgc cgggatcggc gccctctttt tgacggggga
      841 aaacagtttc gatccggagt tcgtacgcat ggcggggccc gcggccgaag gcgcctacgt
      901 cacctacctg ggccgtccgc ccgaattgct ggacaccgcc aaggccttcg tggacaaata
      961 caaactccga tatcccggcc aggaaatcaa agcctacgac cactacggtt acgagatcac
     1021 gaacatgctc ctggaagccc tggagaaagt cggtcccgac aaagcgaaga tcatcgaata
     1081 tttgcggggc ctgcgttaca ccggtgtgtt gggcgaaacg acctttgacg aaaagggaga
     1141 cacccgaaac cggaccatca ccctgtttgt catcaaaaaa ggcgttttca cccggtccct
     1201 ttagcgtcgt tctggccgcg ctgctgttgg cggcctgcgc cgggcccttc gcccgctccc
     1261 ggttccatcc ggcggccggt cggggtccct cggacccgcc gctcaaaatc cattttcgcc
     1321 tccccttgga acgggcccgg attttgtcgc ccttcgggac ccgtcacggt cgcctgcaca
     1381 cgggcattga tcttctggaa acccggggcg ggggcgatcc cgtgttggcc gccgcggacg
     1441 gccgggtggt ggggatcgga cggggaggac cctacggccg ctcgatcctc ctggagcacg
     1501 ccggcggtta ccgcacgcgc tacgcccacc tacgaaaatc cctggcgcgg cccaacgccc
     1561 acgtggcggc gggggaaaaa atcgggatcg ttggacgcac cggaaacgcc acgacggccc
     1621 atcttcattt cgaagtgatc acgccctccg gccggttttt ggatccgggc ccgctgttgt
     1681 tcccaccctc cccgcgcccc gccgggaagt caaaaaagta gaataactcc gcgtgagtcc
     1741 tctatcgaca accatcgccg aacaattgtt gaacggcttg actctgggcg gcatctacgc
     1801 cttgatcgcc ctgggctaca ccatggtgta cggggtcctg caaatgatca atttcgccca
     1861 ttcggaattg ttcatgtggg ggggcgtcgc gggggcgctt ttcttgagtt tccttccggc
     1921 ggacgccggc gggggggccc tgatcgcggc cttcgtcgtc gccatgggcg cctccgggat
     1981 tctggccatg attctggacc gggtcgccta cgcccccgtt cgccgatcgt cgcggctgac
     2041 ccccctgatc tccgccatcg gcgcctcgct ctttttgcaa aacctggtct tcctttggcg
     2101 ggattcccaa atggccttcc cccaaccgct ggccgtccgg tccttcccct gcggccccgt
     2161 gaccgtgaac ggacttcaag tgttcattgt ggggggctcc gtcgccctca tgggcgccct
     2221 gacgtttttc gtccgccgaa cgaaaatggg gaaagccatg cgcgccgtcg cccaggacgc
     2281 cgacaccgcc gaactgatgg gcattccggt caaccgagtg atcgccctga cctttttcat
     2341 cggcggggcc ctgggcgggg ccgccggcgt gatgaacggc ctatactacg ggtccgtcaa
     2401 atacaacatg gggtttctcc ccggggtgaa agccttcacg gccgcggtcc tggggggcat
     2461 cggcaatctg cccggggcca tgctgggcgg tttcctcctc ggcgtggtgg aagccctggg
     2521 caccgggttc ctaccggaac cggagtggaa agacgttttc gccttcgccg ttttgattct
     2581 ggtccttcta ttccgacctt cggggctcct cggtgagaac accgctgaaa aagtttaaat
     2641 cgtatttttg ggcgggcgtc ggcgtggcgg cgtttctggc cgccccgctc gtcctccagg
     2701 gaacctcctg ggccttcgtg attcgaatcc tggccctgat gggcctttac ggacttctgg
     2761 ccctcggcgt caatttggtg ctgggctacg tgggcctgtt ggatctcggg ttcatggcgt
     2821 tttacgccat cggcgcgtac acaacggccc tgctttccct tcggggggtc ggattttggt
     2881 ggtgccttcc cgccagcgtc ctcgcggccg tgacggttcg aacgcttctg gggtggcccg
     2941 tgctccgctt gcggggcgat tatttgtcca tcgtgaccct ggggttcggg gagatcaccc
     3001 gcatcgcgct caacaattgg gattcgctca ccaacggccc caaagggctt tcccttttgt
     3061 cgtcggccgc ggtgcctccc cttcgtttct ttgggttcgg gatccaaacg aacacccact
     3121 ttttctattt gattttcgct ttgctgagtc tcggactcct ggtgtgcgcg cgcctcaacc
     3181 gctcccggat cggccgggcg tggatggcga tccgggacga cgagttggcc gcccgggcca
     3241 tgggggtcaa cgcgccccgg ctcaagaatc tggccttcgg gctgtccgcc ggtttcgccg
     3301 gcgtggcggg ggccatcttc gcccggtggg aaaacttcgt cacgcccgaa tccttcacgt
     3361 tttgggaatc ggctctcctg gtcgccatgg tggtgttggg cggcatgggg tccctgcccg
     3421 gcgtcctggt gggcgtggcg ttgatcctgg gtcttccgga gattctccgg tccggcgtgt
     3481 ttcgatggat cggcggcgcc caggccgtca acgcccgtta cctggtcttc ggcgcgattc
     3541 tcgtggccat ggcgattttt cggccccagg ggctttggcc ccgccgtgtg gaccggttgc
     3601 cggcggggaa cgaataagtg cgcctcctgg gcgttcgcgg ggtgtccaaa aaattcggcg
     3661 gcgtcgccgc cctggacggc gtggagttcg acatcgaatc cggggagatc gtgagcgtca
     3721 tcgggcccaa cggcgcgggg aaaaccacgc tcttcaacgt cctcaccggg ttttaccggc
     3781 ccgacgccgg cgccatcgag ttttccggcg aacggatcga cgacctgccc tcccaccgga
     3841 tcgccaagcg gggcatcgcc cgaacctttc agaacatccg gctcttcggc cgcatgaccg
     3901 ccctggaaaa cgtcatggtc ggccgccatt gtcgcacccg atcggaactg tggccggccc
     3961 tgctccgcac ccgctctttc taccgggagg agcgcgaaat ccgccattcc gccctcgagc
     4021 ggctgaaggc cgtgggattg gaaaaggaag cccacgccct ggcccgcaac ctcccctacg
     4081 gggaccagcg tcggttggaa atcgcccggg ccctggccgc cgagccccgt cttctgttgt
     4141 tggacgagcc caacgccggc atgaatcccc aggaaagcga agggctgatg gccttgatcc
     4201 gacggattcg gggggaaggg gtgacggttc tgctgatcga gcacgccatg cgagtggtga
     4261 tgggcgtgtc cgaccgcgtc atcgtcttgg actacggcaa aaaaatcgcc gaagggcccc
     4321 cggccgcggt gcagagcgac ccccgggtga tcgaggccta tttgggcgcc ccgcgggagg
     4381 aggacgcccg tgcttgaggt gaaaaacgtc agcgcctcct acggccggaa gctccgcgcg
     4441 cttcacggcg taagtctgac cgtgggtcgg ggcgaaatcg tttgcttgat cggcgccaac
     4501 ggcgccggca agagcactct gctcaagacc ctgtcgggtc tgatctggcc ggacgccgga
     4561 gaaatccttt tcgacggccg ccgcgtggat tcgctgccgg ctcacgccac cgccgaacgg
     4621 ggattgatcc acgttcccga agggcgccgg attttcccgc gaatgaccgt tcgggaaaat
     4681 ctggaaatgg gggcctacac cggcgccgcg gccgaggcgc gacggggaat cgaccgggtg
     4741 gtccgcctgt ttccggtgtt gggagaccgg ttgtcccaat ggggcggcac cctctccggc
     4801 ggggaacagc aaatgttggc catggggcgg gcgctcatgg cccggccccg ccttctcatg
     4861 ctggacgaac cgtccatggg tttggcgccg ctggtcgttc aaaagatctt tgagatcatt
     4921 cgggacatca accgggacgg cacaccgatt ttgttggtgg agcagaacgc caacgaggcc
     4981 ctgcgcgtgg cccaccgggg ctacgtgatt gaaaccggcc gcatcgccca cgaaggctcg
     5041 gcccgggacc tgcggacgga tcccaaggtc cgaaacgcct atttgggggt ttcctgattt
     5101 ccggggggct tcgccggtcc tccaccgctc gggtggaact tgtgtgcgtg gggacggaac
     5161 tgttgacggg taaaatcagc acccacggcg ccgccttcgg ggcgcttttg gaggatcggg
     5221 gatggccgtt gacccgcgaa accacggtgg ccgacgatcc ccgcgagatg gaagccgtct
     5281 tccgggaaat ttggcgacgg tcggacgtga cgatcgtcac cggcgggttg ggcccgacct
     5341 tcgacgacgt aacccgggag gtgtggtcca aggtcacggg acgccggttg cgccggaacc
     5401 gggcggtgga agccgtcatc gcggggcgat ttcgggcgcg cggcctgcgc atgccgcccg
     5461 cgaaccggcg ccaggggatg attctcgatg gggcgaccgt tctgaccaac gacgccggca
     5521 ccgcccccgg acaatgtctc tggaccggca aaaaaatgct cgttttgttg cccggtccgc
     5581 cccgggaact gacgcccatg gtcgaaaacc attggctccc gctttttcgt cggcatttcc
     5641 ccgacggtcg ccgcgccacc cgggtgtggc ggtttttcgg cctatcggaa agcgccgtgg
     5701 acgaggtgtt gcgttccctc gttcggcgcc ccggggaggt gtggggcatc ctcgcccaat
     5761 cttttttggt cgacgcaaag ttgacgctgg cggacgtcga ccccgcgcgg gcggcgcggc
     5821 gcttcgctga agtcgagaaa tccctgcggc gggccttcgg ggcggctctt ttcggaacgg
     5881 ggcgggagac gatggaggaa tagtcgtagc ggccgtggtt gcgcagcatg g
//
LOCUS       DAOQLS010000028         5927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5841_length_5927_cov_5.590940, whole genome shotgun sequence.
ACCESSION   DAOQLS010000028 DAOQLS010000000
VERSION     DAOQLS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5841_length_5927_cov_5.590940"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK362_00975"
     CDS             complement(<1..376)
                     /locus_tag="PK362_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73446.1"
                     /translation="MIYKRSILAAVAACSLASGCGKKEAPAPKVETFPVRVETVERRT
                     LEETLALVGSLKAKDEATLFSRVPGKLKQNLVKEGDPVRKDQAVALVERDEVGVHFEP
                     APVPSTLRGVVARVYLDRGANVT"
     gene            complement(373..1623)
                     /locus_tag="PK362_00980"
     CDS             complement(373..1623)
                     /locus_tag="PK362_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73447.1"
                     /translation="MTLTLEAAVELAMKENIDVALAELDLRAFQSRYREVIGAAISDL
                     SLTGAYTRNFEKRIAFFSDGKTEIGERNSMVGGVEMEQPLYSGGKLRAGLKAAKLGTA
                     AGEDALRSRRADVKLVVRSFFYTVLLASATASISEDTLRSAEAHLYTIRERYKQGLDS
                     DLTVLRQEVEAANAKPALIQSRNLFELGLTSLKDVLGLDVDRPIIIDGRLDPPDFAAP
                     SYESLVNSALAHNPDYQAARNRAEAALQLARATAGDQRPQLSLFGAYLWTAESPDFSP
                     GSSQRGIIAAGGLRLRYPFFTGGEVLERVRQARIDHERALEVQRKTERTVRVEVKKQW
                     LAAKEAEERARSQEGAIGQARRALDATEVRYKSGDASQLELNDATLALGRARTLYAQA
                     SHDFLVALAALERAAGTRLEEINR"
     gene            complement(1700..2266)
                     /locus_tag="PK362_00985"
     CDS             complement(1700..2266)
                     /locus_tag="PK362_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC73448.1"
                     /translation="MDAARKSLITRGYHDVHLDDVAVTAGVSKGAIYLYFKDKESMFS
                     AVLKDVIDQLEERLKALPTQGTALDVLSRMAAEMLDFVDEHQDFLIQFSREKPDLCGK
                     RAGTVLQERFAGHLRFVANAVERCFKEGSLRRHDPALGGLFFISLVRMFMLQKIMGQA
                     AGPLRPKADQLMDLFLHGLGGRGARPRA"
     gene            complement(3634..4581)
                     /locus_tag="PK362_00990"
     CDS             complement(3634..4581)
                     /locus_tag="PK362_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNC73449.1"
                     /translation="MTHETFVGIDVSKDRLDIALRPTEESWSVPSDEASIASLVDRLK
                     AIKPTLIVVEATGGFEVPVSSALAAAALPVVVINPRQVRDFAKSTGRLAKTDAIDAQV
                     LAQFGQAVRPPIRPLSDAQAKELQGLVVRRRQIVEMIVSEKNHFRAALPTLRPEIKKH
                     IQWLQQRLIQMNDQLQALIREMPIWREKDDLLRSAPGVGPQLSVSLISGLPELGSLGR
                     KQIAALVGVAPMNRDSGAFRGRRTVWGGRAHIRATLYMVTLTAIRKNPSIRDFYQRLR
                     KAGKAPKVAITACMRKLLTILNAMMKHKTPYRENFAYAS"
     gene            5650..>5927
                     /locus_tag="PK362_00995"
     CDS             5650..>5927
                     /locus_tag="PK362_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73450.1"
                     /translation="MINRHVVMIDNHGGGARGGAVISAGDSGQRSKNGEPLTRLSATL
                     SPGGEGFIWLTAYPVEYRIPSSSGSCPNNHAQYWEKTGAQFETEGSPCS"
BASE COUNT         1165 a         1688 c         1939 g         1135 t
ORIGIN      
        1 gggtcacgtt cgcgccgcgg tccaggtaga cgcgggcgac gacccctctc agggtggaag
       61 ggacaggggc gggctcgaag tggaccccca cctcgtcgcg ctcgacgagc gccacggcct
      121 ggtccttgcg cacggggtcg ccctccttga ccaggttctg cttgagtttg ccgggaaccc
      181 gggagaaaag cgtcgcctcg tccttggcct tgagcgagcc caccagcgcc aacgtctcct
      241 cgagcgtcct gcgttcgact gtctcgacgc ggacggggaa cgtctcaacc ttgggagccg
      301 gagcctcctt cttgccgcag ccggatgcca gcgaacacgc ggccacagcc gccaaaatcg
      361 atcgtttata aatcatcggt tgatctcctc cagtcttgtg cccgccgcgc gttccaaggc
      421 cgccaacgcc accagaaaat cgtgcgaggc ttgtgcgtag agcgtgcgcg cgcggccgag
      481 cgccagcgtg gcgtcgttga gctcgagttg gctcgcgtct ccgctcttgt agcgaacctc
      541 cgtggcgtcc aaagcgcgcc gcgcctgtcc gatggctcct tcttgcgagc gcgcccgctc
      601 ctcggcctcc ttggcggcca gccactgctt cttgacctcg acccggacgg tgcgttcggt
      661 cttgcgctgg acttcgaggg cgcgctcgtg gtcgatgcgc gcctgtctca cccgctcgag
      721 gacctctccg cccgtgaaga acgggtagcg caggcgaagc ccacccgcgg cgatgattcc
      781 tctttgcgag gagccgggag agaagtcggg cgattcggcg gtccaaaggt aggcgccgaa
      841 cagtgaaagt tggggccgct gatcgcccgc cgttgcccgg gccagctgga gcgccgcctc
      901 agcgcgattg cgcgccgcct gatagtcggg attgtgcgcc agggccgaat tgaccagaga
      961 ctcatatgag ggcgcggcga agtcgggcgg atcaagacgg ccgtcaatga taatcggtcg
     1021 gtccacgtcg agtccgagaa cgtccttaag agacgtcagg cccagttcga agaggtttcg
     1081 ggactggatg agcgccggtt tggcgttggc cgcctcgacc tcttggcgca agacggtgag
     1141 gtcgctgtcg agcccctgct tgtagcgctc gcggatcgtg tacagatgcg cctcggcgga
     1201 gcgaagggtg tcctcagaga tggacgccgt cgcgctggcc aggagcacgg tgtagaagaa
     1261 gctccgcacc accagcttga cgtccgcgcg cctggagcgc aaggcgtctt cgccggccgc
     1321 cgtcccgagc ttcgcggcct tgaggcccgc ccggagcttg ccgccggagt aaagcggctg
     1381 ctccatttcg acgccgccca ccatgctgtt gcgttcaccg atctcggtct tgccgtcgga
     1441 aaagaaggcg atcctcttct cgaagttccg cgtgtaggcc ccggtcagcg aaagatcgga
     1501 gattgccgcg ccaatgacct cgcggtagcg gctttgaaag gcccgcaggt caagttccgc
     1561 aagggccacg tcgatgttct ccttcatggc cagttcgacg gccgcctcca gcgtcaaggt
     1621 caagggctcc ggtggcgcgg ccggttccgc gcgcgccgcg ccggaaaggg ccacccacgc
     1681 cagcgccgct aaaagcgtac tacgcacgag gcctcgcccc ccgtccaccg agaccgtgga
     1741 ggaaaagatc catgagctgg tccgccttcg ggcgaagcgg gccggcggct tgccccatga
     1801 tcttctgaag catgaacatc cgtaccagcg agatgaaaaa caggcccccg agggccggat
     1861 cgtgccgccg aaggctcccc tccttgaagc aacgctcgac agcgttagcc acgaaacgca
     1921 ggtgcccggc gaaccgctcc tggagcaccg tccctgcgcg cttgccgcac aagtccggct
     1981 tttcccgcga gaattggatg aggaagtcct ggtgctcatc cacgaagtcg agcatctcgg
     2041 ccgccatgcg cgaaagaacg tccaacgccg tcccctgcgt cggcagcgcc ttcaaccgct
     2101 cttccagttg gtcgatgaca tccttgagca ccgccgagaa catgctctct ttgtccttaa
     2161 aataaagata aatcgccccc ttggatacgc ccgccgtaac ggccacatcg tcgagatgca
     2221 cgtcgtggta cccccgggtg atcagcgatt tccgggcggc gtccaatatc tcttggcggc
     2281 ggatcgactt tggctttgtc ctggcacgag ccatggggat atccctccat ttatactgac
     2341 tgaatgttca gtcagtaata taacgcatgc cctatccttt gtcaatgggg tgcgggttcg
     2401 acacacgttt gtttttcgcc ctttccgcac cgcccgctat ccgcctcggg agatgaggat
     2461 aattcgactt actgtgccgt tggaaaggaa gtattcctgt ccaggcacgc ggaatttccc
     2521 gacgcgacat cgaacaggat gtcacacgtc aatgcccaag aaaatggggg cggtatgaat
     2581 attttcggca gccgtcttta aaatccgaat agagcagttc tgcgtggatc gagaatccat
     2641 taccggtaag gagggcgaac cgttcctgtc caggatcgtc catgctgcct ccacgaattc
     2701 ccatttcgtg ggctaaacca ttgcgtaact ctttccacat ccatccaggt aaatctgcgg
     2761 tcggagtggt ccagagaggg aggtattggc ctagaaaggc cctaaagcac tttaggttct
     2821 catcagtatt gttgagtaag tattcaccca tcgcttcaat tccacagcaa acaaggcttg
     2881 tcccaagaat gaaaagcgat cattcgggtc cattgatggc atagtgagct ggcgctcttt
     2941 ttagaaaaac ttggtccatc gagtgaaaga aaatcaagtc catccgtttc tgaaaataga
     3001 gaattttgtc ttcgataggg agaggtggat tggttgacga taggatggga aaatcggcgt
     3061 ctttccagcc actgcatgac tgcttcgtgc tggggagttt gagtcggcca agtttgagat
     3121 aggcgtagag ccagcgttga aatttgacaa gcgcattggg aataaaccaa atcataaatg
     3181 aattttattg gattaaccta tgcggagaaa aggaggattg gcgcgcgaag cgcgcccgca
     3241 aggtttggtg tttttaatgg gggcccatgc cacctattga acttgggggg cggggggcat
     3301 ggagacgcgg ggggagcgag gcgataacgg cccgccacag cggcagcgaa ggcgggcagg
     3361 gcgggtccga acccgccctg agtgccgagc ggcagtggcc ccctaccccc tacacatggc
     3421 acgcgccccc ggcggcggat tgcgaagcga aagccgacgc cggacgggag accccgaaga
     3481 cgatttaatg cgcataggcg gccaacgagg accgtgccgt ttggccgcca gggttggggg
     3541 ccgtggcggc caggaccaca ggaggccgcc gtctcaacgg ccggaggaaa aacgaaagga
     3601 aacggcgcgt ggggggagca actgtcatga aaatcaagag gcgtaggcga agttttcccg
     3661 atagggggtt ttgtgcttca tcatggcgtt gaggatggtt aagagcttgc gcatgcaggc
     3721 ggtgatggcg acctttgggg ctttgccggc tttgcgaagg cgctggtaga agtcgcggat
     3781 ggatggattc tttctaatgg ctgttaaggt gaccatgtaa agcgtggcgc gaatatgggc
     3841 gcggccgccc caaacggtgc gcctgccccg gaaagcgccg ctgtcgcgat tcatgggtgc
     3901 gacaccgaca agtgcggcga tttgtttgcg gccaagcgag ccgagctccg gcaggccgga
     3961 gatgagcgac acggacaatt gcggaccgac gcctggggct gagcgaagaa ggtcgtcctt
     4021 ttcccgccaa atcggcattt cacggataag ggcctggagt tgatcgttca tttggatcaa
     4081 tcgttgctga agccattgga tatgtttctt gatttcaggc cggagcgtag ggagagcggc
     4141 cctaaagtgg ttcttttcgc tgacgatcat ttcgacgatt tgccgacgac ggacgaccag
     4201 gccttgcagc tccttagcct gggcatcgga cagaggtcgg atgggcggac gaacggcctg
     4261 gccgaattgc gcgaggacct gggcgtcgat ggcatcggtt ttggcaagcc ggccggtgga
     4321 tttggcgaag tcacggactt ggcgagggtt gatgacgacg accgggaggg cggccgcggc
     4381 cagggcggag gaaacgggga cttcgaaacc tcctgtggct tctacaacga tgagggtggg
     4441 tttgatggct ttaagtcggt cgacaaggga agcgatggaa gcttcatcgg aggggaccga
     4501 ccaagactcc tcggtgggcc tgagggcgat atccagccgg tctttggaga cgtcgatgcc
     4561 tacgaatgtt tcatgagtca taaaacctcc ttttgtcccg tccttgcgaa tgcgggctta
     4621 gagcccaagc aactgttcgg gatgctgagg ataaagcgga cgtggcgacc catgctcccc
     4681 cgcgatctct aagaaccaag ggggccgcgg cctgccacgc cgtatcttgg gaactgctgg
     4741 agcagtagac caagacgaat ttcatgatac aaggggggcc ggtgtaagcc tggcagcccc
     4801 ggcagcggtg acgtgcgcgc cgttgattgg cgtgcacggc ggcgcaatgg agccggggct
     4861 tgcggaggcc accgtcaagc gcgggggttg tggtgacttc tgcaacctga cccaatggcg
     4921 acgaacaatg gagctagctt tttgtgagcg ggccccgcgc gaggcgcgac agtggaaccg
     4981 actgagcctt gcccgcaagg cagggcccgc gaatgatttg aagtgcgtcc ccgggggcaa
     5041 tgtatttgac cccggggacg aacaaatagc caggcccgac ccctaccttt gcgcgcgcgg
     5101 agacgggagg gagggccaga ccttaagggc ttggttctct ccaaccaacg gagcgggggg
     5161 cggtccagtc ccgggggcga cggagcggag gtggcgaggc gcatgtgacc gaggcgcggg
     5221 agccgaggaa atggttggag ggcgctagcc cggaaaccat gccgagccgc cggagcgacc
     5281 ctgttttaat cgaaccaacc gaagcggtca gcgtccgggt tttcccgggg agcgaatcaa
     5341 ccggagtggg gtcttttctt ttcgaccgga gcgagcgtgg ttcagtcgcg ccagcaaccg
     5401 agcgagccag tgcggcggtg cttgctccgc cgcgcggcga gcgccccggg gtggaacgat
     5461 tcaaccgcag cgaccagcgc ccgggggttt tccgggggga agcgaatcaa ccggagcgga
     5521 gacttccttc cgatcgaagg ccggggttgt ccttcttctt tgtctccggg ataacagggt
     5581 ttgacgagcg gggcggtctt gggtttttcg gaggcgggac ttcagggttt taacgcggag
     5641 gcggcaccga tgatcaatcg ccatgttgtc atgatagaca accacggtgg cggagcgcgg
     5701 ggcggcgcgg tgatttccgc cggcgatagc ggtcaacggt caaaaaacgg cgaacccctc
     5761 acccggcttt cagccaccct ctccccaggg ggcgagggat ttatttggtt gacggcttat
     5821 ccggtcgaat atagaattcc ttcgtcaagc ggtagttgtc ccaataatca tgcgcaatat
     5881 tgggaaaaga caggggcaca gttcgagaca gaagggagtc cctgctc
//
LOCUS       DAOQLS010000029         5920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5856_length_5920_cov_5.727366, whole genome shotgun sequence.
ACCESSION   DAOQLS010000029 DAOQLS010000000
VERSION     DAOQLS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_5920_cov_5.727366"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..343
                     /gene="rpsS"
                     /locus_tag="PK362_01000"
     CDS             56..343
                     /gene="rpsS"
                     /locus_tag="PK362_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNC73451.1"
                     /translation="MSRSQKKGPFVDSNLQEKVQAARAAGDKKPIKTWARASMISPEF
                     VGLTIAIHNGKKHQPIFITEQMVGHRLGEFAPTRYFRGHGEAHTKEAAAKT"
     gene            353..679
                     /gene="rplV"
                     /locus_tag="PK362_01005"
     CDS             353..679
                     /gene="rplV"
                     /locus_tag="PK362_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNC73452.1"
                     /translation="MQAIAHARFARYSYRKVGQVLGLIRGKSVVDAMGMLPWVPRACR
                     VLVGKTLKSAVANAGKNADPARLKVAQAWVNHGPALKRVMPKAMGARAMFKRKTSHLT
                     ITVSDN"
     gene            700..1542
                     /gene="rpsC"
                     /locus_tag="PK362_01010"
     CDS             700..1542
                     /gene="rpsC"
                     /locus_tag="PK362_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNC73453.1"
                     /translation="MGHKTHPKGIRLGYIRDWDSKWLNLKEMPSLIEEDQKIRKFLKG
                     RLKLAAVSKIGIERTGKYLRVNIYTARPGLVIGKKGADIEGLRSVIEDMTSLKTRIEI
                     IEIKKPEVDAQLVAEGVAMQLEKQVGFRRAMKRSIERAMQAGALGIKIQVAGRLGGAE
                     IARTEWLKEGRVPLQTFRADIDYGFTEARINMGTIGVKTWVFRKELFKKTDADLMEEV
                     RVIEEKEKEELAKKAEQGLDTAEPVLALEPAADAEVIEEEVMKAVEQEETEARKRTEL
                     EGKK"
     gene            1544..1957
                     /gene="rplP"
                     /locus_tag="PK362_01015"
     CDS             1544..1957
                     /gene="rplP"
                     /locus_tag="PK362_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNC73454.1"
                     /translation="MLMPTRVKYRKSQRGRMKGCTKAGAFVSFGEFGLQALEAHWITA
                     RQIEAVRVTLARFLKKGGKVWIRIFPDKPVSKKPAETRMGKGKGNPEFWVAVVKPGRV
                     LFEIEGVTAEVAKQAMTLAGHKLPIKTRFVQRDHF"
     gene            1969..2175
                     /gene="rpmC"
                     /locus_tag="PK362_01020"
     CDS             1969..2175
                     /gene="rpmC"
                     /locus_tag="PK362_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018017485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNC73455.1"
                     /translation="MAKKKEDVNFGELSKDELRVKLQESRDKLFQLRFQNATAPLKNH
                     REIRTTRRNIARAMTFLKQKGDNA"
     gene            2172..2477
                     /gene="rpsQ"
                     /locus_tag="PK362_01025"
     CDS             2172..2477
                     /gene="rpsQ"
                     /locus_tag="PK362_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNC73456.1"
                     /translation="MTTKVERADRKTLEGVVLSDKMNKTRVVEVERRLRHALYGKVLI
                     RTSKVYAHDENNESKAGDRISVMATRALSKQKRWRLVKILERAGVAQGVDASLGGKS"
     gene            2474..2842
                     /gene="rplN"
                     /locus_tag="PK362_01030"
     CDS             2474..2842
                     /gene="rplN"
                     /locus_tag="PK362_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNC73457.1"
                     /translation="MIQERSILKVADNSGARLVRVFRMHGGSRRRYAYLGDVVTASVQ
                     NALADSNIKKGEVVKCVVVRTRKEKRRPDGSYVRFDDNAGVLIDENGEPRGTRIFGPI
                     ARELREKNYLKIISLAPEVV"
     gene            2842..3159
                     /gene="rplX"
                     /locus_tag="PK362_01035"
     CDS             2842..3159
                     /gene="rplX"
                     /locus_tag="PK362_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNC73458.1"
                     /translation="MALATVRKKDKVRILAGKDRGKEGEVLDVDLAGRRVLVAKLNLV
                     KRHTKGTPQNPGGVKEQEAFLPMSKVMLVCPKCKRTARPKAGALSDGTKARVCRNCGE
                     MVG"
     gene            3173..3766
                     /gene="rplE"
                     /locus_tag="PK362_01040"
     CDS             3173..3766
                     /gene="rplE"
                     /locus_tag="PK362_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNC73459.1"
                     /translation="MSTATKSTAPEPRLKAAFQKQVVPEMMKKFSWENPFQVPRVKKI
                     VVNIGVSEARENAKVMDLAADELTAIAGQKAEVRRSKKSISNFKLREGMPIGLRVTLR
                     GARMWEFLDRLVNVAMPRIRDFQGLDPRRGFDGRGNFNLGLTEQYVFPEISLDKSDKA
                     RGMNITIVTDAQRDDTARELLILLGMPFKRDRKAALN"
     gene            3777..3962
                     /locus_tag="PK362_01045"
     CDS             3777..3962
                     /locus_tag="PK362_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNC73460.1"
                     /translation="MATKAWVAKTRKPQKFAVRHRSRCRLCGRPRGFYRDFGLCRICL
                     RTLALRGELPGVTKSSW"
     gene            3992..4387
                     /gene="rpsH"
                     /locus_tag="PK362_01050"
     CDS             3992..4387
                     /gene="rpsH"
                     /locus_tag="PK362_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNC73461.1"
                     /translation="MSFDPVSDMFAMITNANHKFMERIDLPSSSLKKEVAHILKEEGY
                     IADYKVLPDRKQGVLRIFMKYLPNKTRVIRGVKRISRPGLRQYKGYEKLGKLRGGLGI
                     SIVSTSQGLMTDRQSRKAKLGGEVLAQVW"
     gene            4392..4976
                     /gene="rplF"
                     /locus_tag="PK362_01055"
     CDS             4392..4976
                     /gene="rplF"
                     /locus_tag="PK362_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002787868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNC73462.1"
                     /translation="MSRLGRKPIVLPEKVKVSVDGPKVSVAGPLGTLTRALPQGISAK
                     VDGGVLTVARANDSTTQRALHGTLRKLLLNMVEGTAKGFQKELRIGGVGFRATLTGNH
                     LHMTLGYSHPIEYTVPAGIKLTVDAKQTTIVVQGADKELVGQVAAEIREFRKPGVYWA
                     NNEPVGIRYANEVVRRKAGKAAAGAAGGAAGAKK"
     gene            4973..5332
                     /gene="rplR"
                     /locus_tag="PK362_01060"
     CDS             4973..5332
                     /gene="rplR"
                     /locus_tag="PK362_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016477826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNC73463.1"
                     /translation="MSLKSPQARYAFRKARTRAKVFGTAARPRLSVFRSQKHIYAQII
                     DDVAGKTLAAASSVMPGDKKALGATVSAAEKIGRSIGERAKGLNISQVVFDRGGRPYH
                     GRIKAVADGARAAGLQF"
     gene            5344..5868
                     /gene="rpsE"
                     /locus_tag="PK362_01065"
     CDS             5344..5868
                     /gene="rpsE"
                     /locus_tag="PK362_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNC73464.1"
                     /translation="MARIDANSLNLKETVVAINRVAKVVKGGKRFSFNALVVVGDQAG
                     HVGAAMGKAKEVQAAIQKAVQHAKKTIIRAPLKGATIPHEVTGIFGAGTVLIKPAAPG
                     TGVIAGGSVRAVLEAVGVRDVLTKSLRSANPFNVVYATIEALKNLQTKEDVARLRGKT
                     LPASTTAAPAPAGV"
BASE COUNT         1320 a         1857 c         1863 g          880 t
ORIGIN      
        1 ctggatgatc atccgccgcc gcaccgacgg cgccgagcaa gcgtaaggag cccccatgag
       61 ccgttctcag aaaaagggcc cgttcgtcga ctcgaacctg caggaaaagg ttcaagccgc
      121 ccgcgcggcc ggcgacaaaa agcccatcaa gacctgggcc cgggccagca tgatcagccc
      181 cgaattcgtg ggcttgacga tcgcgatcca caacggcaaa aagcaccagc cgattttcat
      241 caccgagcag atggtgggcc accgcctggg cgagttcgcc cccacccgct atttccgagg
      301 ccacggcgaa gcgcacacca aagaagccgc ggcgaaaacg taaggacgac ctatgcaagc
      361 cattgcgcac gctcgattcg cccgatattc ctaccggaag gtgggccagg tgttggggct
      421 catccgcgga aagtccgtgg tcgacgccat ggggatgctc ccctgggtcc cccgggcttg
      481 ccgggtgttg gtcggcaaga cgctgaaaag cgccgttgcc aacgcgggga agaacgcgga
      541 ccccgcccgc ctcaaagtgg ctcaggcctg ggtgaaccac ggcccggcgc tcaaacgcgt
      601 gatgcccaaa gcgatgggcg cccgcgcgat gttcaaacgc aaaaccagtc atctcacgat
      661 caccgtgagc gacaattagc gaagggataa cactttctca tgggtcacaa aacacacccc
      721 aaaggcatcc ggctcggcta catccgcgac tgggattcga agtggctgaa cctcaaggag
      781 atgccctcct tgatcgaaga ggatcagaag atccgaaaat ttttgaaggg ccgcctgaag
      841 ctggccgcgg tctccaaaat cggcatcgaa cgcacgggca agtacctgcg cgtcaacatt
      901 tacaccgcgc gccccggttt ggtcatcggt aaaaaaggcg ccgacatcga aggcctgcgg
      961 tccgtcatcg aagacatgac gagcctcaag acccgcatcg aaatcatcga gatcaagaag
     1021 cccgaagtcg acgcccagct ggtggccgaa ggcgtggcca tgcagttgga aaagcaggtc
     1081 ggtttccgcc gcgccatgaa gcgctccatc gagcgcgcca tgcaggccgg ggccctcggg
     1141 atcaagatcc aggtggccgg ccgtctgggc ggcgccgaaa tcgcccgcac cgaatggctc
     1201 aaggaaggcc gcgtgccgct ccaaaccttc cgggccgaca tcgactacgg tttcaccgaa
     1261 gcccgcatca acatgggcac catcggcgtc aaaacctggg tgttccggaa ggaactcttc
     1321 aagaagaccg acgcggacct catggaagaa gtgcgcgtca tcgaagagaa ggaaaaggaa
     1381 gaattggcca agaaagccga gcaggggctg gacacggcgg aacccgtgct ggcgctcgag
     1441 cccgccgccg acgccgaagt gatcgaagaa gaggtgatga aggccgtcga gcaggaagaa
     1501 acggaagcgc gcaagcgcac cgagctggaa gggaagaaat aacatgttga tgccgacccg
     1561 ggtcaaatac cgcaaatccc aacgcggccg catgaagggc tgcaccaagg ccggggcctt
     1621 cgtgtccttc ggcgagttcg gcctccaggc cctggaagcc cattggatca ccgcccggca
     1681 gatcgaagcc gtccgcgtga ccctggcgcg atttttgaaa aaaggcggca aagtctggat
     1741 ccgcattttc cccgacaagc ccgtgtccaa gaagccggcc gaaacccgga tgggaaaagg
     1801 aaaaggcaac cccgagttct gggtggccgt cgtgaagccg ggccgcgtcc tgtttgaaat
     1861 agaaggcgtg accgccgagg tggcgaagca ggccatgacc ctggccggcc acaagctgcc
     1921 catcaaaacg cgctttgtcc agcgcgatca tttctaagag agcgcgccat ggcgaaaaag
     1981 aaagaagacg tgaatttcgg ggaattgtcg aaggacgaac tccgggtgaa actgcaggaa
     2041 tcgcgggaca agctttttca gcttcgcttt caaaacgcga cggccccgtt gaaaaaccat
     2101 cgggaaatcc gcaccacccg gcggaacatc gcccgggcga tgacgttttt gaagcagaaa
     2161 ggggacaacg cgtgacgacc aaagtggaac gcgccgatcg caagacgttg gaaggggtgg
     2221 tcctttccga caaaatgaac aagacccgcg tggtggaagt ggagcgccgg ttgcggcacg
     2281 cgctgtacgg caaagtgctc atccgcacct ccaaagtcta cgcccacgac gagaacaacg
     2341 aaagcaaagc cggcgaccgg atttcggtga tggccacgcg cgccctctcg aagcagaagc
     2401 ggtggcgcct ggtgaagatc ctggagcggg ccggcgtggc gcaaggcgtc gacgcgtccc
     2461 tgggagggaa gtcgtgattc aagaacgaag cattttgaaa gtcgccgaca actcgggcgc
     2521 ccgcttggtg cgggtgttcc gcatgcacgg cggatcgcgg cgccgctacg cctatctggg
     2581 cgacgtggtg accgcctcgg tgcagaacgc cctggccgac tccaacatca agaagggcga
     2641 agtggtgaag tgcgtggtcg tccgcacccg caaggaaaag cgccgtccgg acggcagcta
     2701 cgtccgcttc gacgacaacg ccggcgtcct gatcgacgag aacggcgagc cgcggggcac
     2761 gcgtattttc ggccccatcg cccgggaact gcgcgaaaag aactatttga agatcatctc
     2821 cctcgctccg gaggtcgtgt aatggccctg gccaccgtac gcaaaaaaga caaagtccgg
     2881 attctggccg ggaaagaccg cggcaaagaa ggggaagtgc tcgacgtcga cctcgcgggg
     2941 cgccgcgtgc tggtcgcgaa actcaatctg gtcaaacgcc acaccaaagg gacgccgcag
     3001 aaccccggcg gcgtcaagga gcaggaagcg ttcctgccca tgtccaaggt gatgctggtt
     3061 tgccccaaat gcaagcggac cgcccggccc aaggccggcg ccttgtccga cggcaccaaa
     3121 gcccgcgtct gccgcaactg cggcgagatg gtcgggtagg ttcgaggaac ccatgtccac
     3181 cgccacgaaa agcaccgccc ctgagccccg gctcaaagcc gccttccaaa agcaggtggt
     3241 tccggagatg atgaagaaat tttcctggga gaaccccttc caggtgccgc gcgtcaaaaa
     3301 gatcgtcgtc aacatcggcg tcagcgaagc gcgcgaaaac gccaaggtca tggacctggc
     3361 cgccgacgaa ttgaccgcca tcgcgggcca aaaagccgag gtgcggcgtt ccaaaaaatc
     3421 catctcgaac ttcaaacttc gggaaggcat gcccatcggc ctgcgcgtca cgctgcgcgg
     3481 cgcccgcatg tgggaattcc tggaccggct ggtgaacgtg gccatgcccc gcatccggga
     3541 cttccagggc ctcgacccgc ggcgcgggtt cgacggccgg ggcaatttca atctggggct
     3601 caccgagcag tacgtgttcc ccgaaatcag tttggacaaa tcggacaagg cgcgcggcat
     3661 gaacatcacg atcgtgaccg acgcccagcg ggacgacacc gcccgggagc tcttgatcct
     3721 cctcggcatg ccgttcaagc gcgaccgcaa agcggctttg aactaaggag acaccgatgg
     3781 cgacgaaggc gtgggtagcg aaaacgcgga aaccgcagaa attcgcggtc cggcatcgga
     3841 gccggtgccg gctgtgcgga cggccccggg gtttctatcg ggattttggg ttgtgccgga
     3901 tttgcttgag aaccttggcg ctccgcgggg aactgcccgg cgtcacgaag tccagttggt
     3961 agttacttca cacaaagaga gacgatgagt tatgagtttt gatcccgtca gcgacatgtt
     4021 tgccatgatc accaacgcga accataaatt tatggaacgc atcgatcttc cttcgtcgag
     4081 cctgaagaag gaagtggccc atatcttgaa ggaagaaggg tacatcgccg actacaaggt
     4141 gctccccgac cgcaagcagg gcgtcctccg cattttcatg aaatacctcc cgaacaagac
     4201 ccgcgtcatc cgcggcgtca agcgcatttc ccgtcccggc ctccgccagt acaagggcta
     4261 cgagaagctg ggcaaactgc gcggcgggct gggcatttcc atcgtctcga cgtcccaggg
     4321 cctgatgacc gaccgccaat cgcgcaaagc gaagctgggc ggcgaagtcc tcgcccaggt
     4381 gtggtgatcc cgtgagccgc ctcggtcgca aacccatcgt cctgccggag aaggtcaaag
     4441 tttccgttga cggtcccaaa gtcagcgtgg ccggccccct gggcacgctc acgcgggcgc
     4501 tcccccaggg catttccgcc aaggtggacg gcggcgtcct cacggtggcc cgggccaacg
     4561 acagcaccac ccagcgggcc ctgcacggca ccctccgcaa gcttctgctc aacatggtgg
     4621 aaggcaccgc caaggggttc cagaaggagc tccgcatcgg cggcgtcggt ttccgcgcga
     4681 cgctgacggg caaccatttg cacatgaccc tggggtattc ccaccccatc gaatacaccg
     4741 tcccggcggg catcaagctg acggtggacg ccaagcagac gaccatcgtc gtgcagggcg
     4801 cggacaagga gctggtgggc caggtggccg ccgagatccg tgaatttcga aagcccggcg
     4861 tttactgggc caacaacgag cccgtgggca tccgctacgc caacgaagtg gtgcggcgca
     4921 aagccggcaa agccgccgcc ggcgcggcgg gcggagcggc aggagcgaag aaatgagcct
     4981 caagtccccc caagcgcgtt acgcgttccg caaggcgcgc acccgggcga aagtgttcgg
     5041 caccgcggcg cgtccgcggt tgtcggtctt ccgctcgcag aaacacattt acgcccaaat
     5101 catcgacgac gtggccggca agaccctggc cgcggcctcg tcggtcatgc cgggcgacaa
     5161 gaaagccctc ggggccacgg tgtcggccgc cgagaaaatc gggcgttcca tcggggaacg
     5221 ggccaagggc ctgaacatct cccaggtcgt tttcgaccgg ggcggtcggc cctaccacgg
     5281 acggatcaaa gcggtcgccg acggcgcccg cgccgccgga ctgcagtttt aaggagatca
     5341 cgcgtggcgc gaatcgatgc aaacagcttg aacctgaaag aaaccgtcgt ggccatcaac
     5401 cgggtggcca aagtggtgaa gggcggtaag cgattttcct tcaacgcgtt ggtggtggtc
     5461 ggcgaccagg ccggccacgt cggcgcggcc atgggcaagg ccaaggaagt ccaggccgcc
     5521 attcaaaagg ccgtgcagca cgccaagaag acgatcatcc gcgccccgct gaagggcgcg
     5581 acgatccccc acgaagtgac cggcattttc ggcgcgggca cggtgttgat caagcccgcc
     5641 gccccgggaa ccggcgtcat cgcgggcggc agcgtccgcg cggtgctgga agccgtcggc
     5701 gtccgggacg tgctcacgaa gtcgcttcgc agcgccaacc cgttcaacgt ggtgtacgcc
     5761 accatcgaag cgctcaagaa tcttcaaacg aaagaagacg tggcccgtct ccggggcaaa
     5821 acgctcccgg cgtcgacgac ggccgccccc gctcccgcgg gcgtctaacg aggacgacca
     5881 tgattcgcgt cacgaccatc aaacccgcgc cgggctcccg
//
LOCUS       DAOQLS010000030         5909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5866_length_5909_cov_4.783738, whole genome shotgun sequence.
ACCESSION   DAOQLS010000030 DAOQLS010000000
VERSION     DAOQLS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5866_length_5909_cov_4.783738"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK362_01070"
     CDS             complement(<1..264)
                     /locus_tag="PK362_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNC73465.1"
                     /translation="MKVLAVIPARHAAQRFPGKPLALLAGRPMVRWVYEAARRALPGA
                     VVVATDDERIRSAVRAFGGEAVMTSERCRSGTDRVAEVARRIRA"
     gene            complement(261..1247)
                     /gene="rfaE1"
                     /locus_tag="PK362_01075"
     CDS             complement(261..1247)
                     /gene="rfaE1"
                     /locus_tag="PK362_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNC73466.1"
                     /translation="MTSPLLSRVSRFAGMPVLVVGDLMVDRFLRGAVHRLSPEAPVPV
                     VDVREDKSMPGGAGNVAANIAALGGRPLLVSVVGEDPEGDRLVDALRAVRVDVDGIVA
                     DAHRPTIVKTRVIAGHQQVVRFDREDRAPLAPAVLNRLLERMREKMTAARAVILSDYG
                     KGVIGPRLLNAVLTLAHRQKKFVMVDPKVEHFLRYRRVDCITPNLKEATEGLRGLPPK
                     NDAEVDALGGRILRRLRCHSVLITRGERGMSLYREGKPPLHIPSEAREVFDVTGAGDT
                     VISTLSLALAAGANLEDAARLSNAAAGVVVGKLGTATVSPAELLAALRARRP"
     gene            complement(1372..2220)
                     /locus_tag="PK362_01080"
     CDS             complement(1372..2220)
                     /locus_tag="PK362_01080"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNC73467.1"
                     /translation="MAERGAAFFAALLLLGAGAAVAEETPAPPAFVWRQKPNTVFTVG
                     ESIVYAIKYGIVPAGTARLDVRAEETIKGRPAFRIECVARSNAAMDAVFKVRDRNESW
                     MDRESLCSLRFRQDMREGLFKRKVETVYDPVTGRFLYRKWRKGKEFVYDGPSPTYIQD
                     VLSSLYYIRTCELKVGESVLLDANSGSNNWTLRVNVIARETVEVPAGRFVCLKLEPVL
                     AGDGLFRSKGRLEVWVTEDARRVPVLLRSRVTVGAFDAEMTEYTPGGGEVPTVAGATS
                     VQTLRP"
     gene            complement(2213..2767)
                     /locus_tag="PK362_01085"
     CDS             complement(2213..2767)
                     /locus_tag="PK362_01085"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNC73468.1"
                     /translation="MIARFRVFMGFIAGVLFLFLSHAGSGLRVGAGLGIALAGLLLRG
                     RAAGYLEKGKRLAQDGPYAWIRHPLYAGSFLMALGFTVAGTSAYYPVHGWILWAVFLI
                     LFVGIYPRRIKEEEASLEKYFGDAWREFTSRNRRFLPRCSPVRRDNPDRFDWARYRKN
                     KEYNAALGWLAGVAVVLIKGALNG"
     gene            complement(2792..4006)
                     /locus_tag="PK362_01090"
     CDS             complement(2792..4006)
                     /locus_tag="PK362_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC73469.1"
                     /translation="MNPPPAGGAARWFERGRSGALAALALTIPLSLAGANLFWGLALG
                     CWLGLLVTDPAARRYRSTGIEIPWLIYLAVELVTALWSDRPAHSLGKVKSEILIVVFL
                     LYAQAPGERRRHVGLFLLGAGIAAALGLVQKSLGWTSEGVHGLARLLDTHRGRAQGFY
                     SNPITYAEMLLFAIFLSLSRRREDGAGFPRWPWAVFALSVAAAAASATRAIWLAGPLA
                     LAVWAFRRKNGRVALALGAVLLGAAAAGVASPAFRARAASITDARTDSSHLIRKGVWV
                     RSLALIREHPWRGVGIGNLHIAGRDLPWGGAAADSDWTEAHNIYLQMAVERGIPGLLV
                     FLGLLAAYGRLLVKAAAAEPEWDGLLFGFLALCLAGLTESWTHDSEVVLCLYMMLGVA
                     ASRSRVAPPHGN"
     gene            complement(4003..5100)
                     /locus_tag="PK362_01095"
     CDS             complement(4003..5100)
                     /locus_tag="PK362_01095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC73470.1"
                     /translation="MIGSPIGIAARDLATSGGRVALELARRLAPNGYEPRLAVHRLGN
                     EPTDAPDGRPVEVATVGEWPFSSWFRAYSFDRSARARLRRRDAQLIHGFGELIVQDLL
                     TLPVLERVRRRHAPGLPPPSAGVGYLRRLQLGPGGARAVTVFSEMARRDLAEAYEVPL
                     ASIRAIPPGVDARRFRPDERGPSRFTLFRASGWTDHYKIVLAVVTDDPATQDFSLLAR
                     AVDLLADRVPSALCVVGRADFSKNAAVQQLARRGRFFHVPVTQHAEKYLVAADVTALP
                     AHYEEFGLPVLESLASGTPVVVSGRTGAKEVLSNGTDGIVVDDMQDPNVWAMALERAW
                     ALDRSVCRRSAEARPWDAHVAAVVQIYRGLM"
     gene            complement(5117..>5909)
                     /locus_tag="PK362_01100"
     CDS             complement(5117..>5909)
                     /locus_tag="PK362_01100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC73471.1"
                     /translation="LPAGSGAGWMHERQLRGRRFRLLIANSRLMKNDVEQRFGVPPDR
                     VRVVYPGFDPARFRPEDRTLWREAQRRRWAVGEEETVVGLVTSGDFEKRGLPLFLRAL
                     AGLPPAVRRRVRAVVVGKETRPAPYLNLMRSLDLGDRVQFETPQVHVEALYHALDVYV
                     HPAPFEEFGMSVQEAMACGVPVLTSRRVGASEFWPGAWNAWLAEAPEVSELSARLAAL
                     VEDPLLRRALGAALAPAVRANDWEAHFRSLRACYDEVLDAAGRVE"
BASE COUNT         1134 a         2028 c         1932 g          815 t
ORIGIN      
        1 ggcgcggatt cggcgagcca cttcggccac ccggtccgtg ccgctccggc aacgttccga
       61 ggtcatcacc gcttccccgc cgaaggcgcg gacggccgac cgaatgcgtt cgtcgtcggt
      121 ggccacgacc accgcgccgg gcagagcccg gcgggcggct tcgtacaccc aacggaccat
      181 gggtcggccc gccagaagag ccaagggttt gccgggaaaa cgctgggccg cgtggcgcgc
      241 cgggatgacg gccaggacct tcacgggcgc cgcgccctga gcgccgcgag gagctccgcc
      301 ggggacaccg tggccgtgcc gagtttaccg acgacgacgc cggccgcggc gttggacagt
      361 cgcgcggcgt cttccaagtt cgcccccgcc gccaaggcca ggctcaacgt ggaaatcacg
      421 gtgtcgcccg cgccggtcac gtcgaacacc tcccgggcct cggaaggaat gtggaggggc
      481 ggcttcccct cccggtagag gctcatgccg cgctcgccgc gggtgatcaa gacggaatga
      541 cagcggagac ggcgaaggat gcgcccgccc agggcgtcga cctcggcgtc gtttttcggg
      601 ggaaggcccc ggaggccctc ggtggcttcc ttcagattgg gggtgatgca atcgacccgt
      661 cgataacgaa gaaaatgctc gaccttcggg tccaccatca cgaatttttt ctgccggtgg
      721 gcgagggtca acacggcgtt caacaggcgg gggccgatca cgcctttgcc gtaatcggaa
      781 aggatcacgg cccgggccgc ggtcattttc tcccgcatcc gctccaggag gcgattcagg
      841 accgccggcg ccaggggggc ccggtcttcc cggtcaaagc gcaccacctg ctggtggccg
      901 gcgatcaccc gggttttgac gatggtcgga cggtgggcgt cggccacgat cccgtcgacg
      961 tccacccgga cggcgcgcag ggcgtccacg aggcggtccc cctccgggtc ttcgcccacc
     1021 acggagacca acagcggccg gccgcccagg gcggcgatgt tggccgccac gttgccggcc
     1081 ccgccgggca tggatttgtc ctcacggacg tccacgacgg gcacgggggc ctccggcgac
     1141 aaacggtgga ccgcgcccct caggaaacgg tccaccatca aatcgcccac caccaggacc
     1201 ggcatgccgg caaaacggga cacccgggag aggaggggag aggtcacggt cggaccgtgc
     1261 gggagaaaac ggaatggggg cacaacgggt cgttcacgcg tgaattccta aagggccgaa
     1321 ccaacggtgc ttgggacgga cggattatac cacatcgccc gcgccggccc atcaggggcg
     1381 cagggtttga acgctggtcg cgccggcgac ggtcggcacc tcgcccccgc ccggcgtgta
     1441 ttcggtcatt tccgcgtcga aagcgcccac ggtcacgcgg gaacgcaaca ggacgggaac
     1501 gcggcgggcg tcctcggtga cccacacttc caaacgccct ttggaccgaa acaaaccgtc
     1561 gccggccagc acgggctcca gtttcaggca cacgaaccgg ccggcgggca cctccacggt
     1621 ctcccgggcg atgacgttga cgcgaagggt ccaattgttg cttcccgaat tggcgtccaa
     1681 caagacggac tcgcccacct tcaattcaca ggtccggatg taataaaggc tggaaaggac
     1741 gtcctgaatg taggtgggcg acggcccgtc gtaaacgaac tctttgccct tccgccattt
     1801 gcggtagagg aaccggcccg tgacgggatc gtagaccgtt tcgactttcc gtttgaacaa
     1861 cccttcgcgc atgtcctggc gaaaacgcag ggaacaaagg ctttcccggt ccatccagga
     1921 ttcgttgcgg tcccggactt tgaacaccgc gtccatggcg gcgttgctgc gggccacgca
     1981 ttcgatccga aacgcggggc gccctttgat tgtttcttcc gcgcggacgt ccaggcgggc
     2041 cgtcccggcg ggaacaatgc cgtatttgat ggcgtaaacg atggattccc ccacggtaaa
     2101 caccgtgttg ggtttttgcc gccagacgaa agcgggcgga gcgggcgtct cctcggcgac
     2161 cgcggcgccg gcgcccagga ggaggagagc ggcgaagaac gccgcccccc gttcagccat
     2221 tcaaagcccc tttgatcaac accaccgcca ccccggccaa ccatcccaac gcggcgttgt
     2281 actccttgtt tttacggtac cgggcccaat cgaaacggtc gggattgtcc cgtcgaacgg
     2341 gggagcaacg gggcagaaac cgccggttcc gggaggtgaa ctcccgccag gcatcgccga
     2401 aatatttttc cagggacgct tcctcctcct tgatccggcg ggggtagatg ccgacgaaga
     2461 ggatcagaaa cacggcccag aggatccaac cgtgaacggg gtaataggcc gaggtgccgg
     2521 ccacggtgaa acccagggcc atgaggaagc ttccggcgta aagagggtgg cgaatccagg
     2581 cgtagggacc gtcctgggcc aggcgctttc ccttttccaa atacccggcg gcccgccccc
     2641 gcaggagcag cccggccaac gcgatcccca atccggcccc cacacgaagg ccggagccgg
     2701 cgtgagaaag gaaaagaaac aaaacgccgg cgataaaacc catgaaaacg cgaaaacgtg
     2761 caatcactca agtctccttg gacgcatttt attaatttcc gtgggggggc gcgacccggg
     2821 atcgggacgc cgccacgccc agcatcatat aaaggcacag gaccacttcc gaatcgtggg
     2881 tccaggattc cgtcaatccg gccaagcaga gcgccaaaaa cccgaaaagg agcccgtccc
     2941 attccggttc ggccgccgcg gccttcacca ataaacggcc gtaggcggcc aaaaggccga
     3001 ggaaaaccaa aaggccgggg atcccacgct ccaccgccat ctggagatag atgttgtggg
     3061 cttcggtcca atccgaatcc gccgcggcgc cgccccaggg caaatcgcgg ccggcgatgt
     3121 gcaagttccc gatgcccacc ccacgccagg ggtgttcccg aatcaacgcc agggaccgga
     3181 cccaaacccc ttttcgaatc agatgggacg agtcggtccg cgcgtcggtg atgctggcgg
     3241 cccgggcccg aaaggccggg gacgccaccc cggcggccgc ggcgcccaac aagaccgcgc
     3301 ccagggccaa cgccacccgc ccgtttttcc gtcggaaagc ccacaccgcc agagccaggg
     3361 gcccggcgag ccagatggcc cgcgtggcgc tcgcggccgc ggcggccacg gacaacgcga
     3421 acaccgccca gggccagcgc gggaatcccg caccgtcctc ccgccgccgg gaaagggaga
     3481 ggaaaatcgc gaacaacaac atctcggcgt aggtgatggg attgctgtaa aacccctggg
     3541 cccgcccccg gtgggtgtcg agcagacggg ccaacccgtg gaccccttcg gacgtccatc
     3601 ccaaactttt ttggacgagc cccagggcgg cggcgattcc ggcgcccagg aggaacaacc
     3661 ccacgtggcg acgacgctcc cccggcgcct gggcgtaaag caggaacacg acgatcaaaa
     3721 tctcgctttt aaccttgccc agactgtggg cgggacggtc ggaccaaagg gcggtgacaa
     3781 gttccaccgc caaataaatc agccagggaa tttcgatccc cgtggaacga taacgccggg
     3841 cggcgggatc cgtgacgagc aaacccaacc aacagcccag ggcgagaccc cagaagaggt
     3901 tcgcccccgc caaggacagg gggatcgtta acgccaaggc cgccagggcg ccggaacgcc
     3961 cccgctcgaa ccagcgggcg gcgccgcccg cgggaggagg gttcacatca gtccccggta
     4021 aatttgaacg accgcggcga cgtgggcgtc ccagggacgg gcctccgccg atcgacggca
     4081 caccgaccgg tcgagggccc aggcgcgttc caacgccatg gcccagacgt tggggtcctg
     4141 catgtcatcg acaacgatac cgtcggtacc gttcgaaagg acttccttgg ccccggtccg
     4201 gccgctcacg accacgggcg tcccggaggc cagggattcc aacaccggca atccgaactc
     4261 ctcgtaatgg gcgggaaggg ccgtgacgtc ggccgccacc aagtattttt ccgcgtgctg
     4321 ggtgaccggc acatggaaaa accgcccgcg gcgagccagt tgctgcaccg ccgcgttttt
     4381 ggagaaatcg gcgcgtccca cgacgcaaag ggccgacggc acccggtccg ccaacagatc
     4441 cacggcgcgg gccaggaggg agaaatcctg ggtggccgga tcgtcggtga cgacggccag
     4501 gacgattttg taatggtccg tccaccccga ggcgcgaaac agggtgaatc ggctgggccc
     4561 ccgttcgtcg gggcgaaaac gccgagcgtc gacgccgggg gggatcgccc gaatggacgc
     4621 cagcggcact tcgtacgcct ccgccaagtc gcggcgggcc atctccgaaa acaccgtgac
     4681 cgcccgggcg ccgccggggc ccaattgcaa acggcggagg tagccaacgc cggccgacgg
     4741 gggcgggagc cccggcgcgt gccgccggcg cacgcgttcc aggaccggga gggtcagcaa
     4801 atcctgaacg atcaactccc cgaatccgtg gatcaattgg gcgtcgcgcc gccgaaggcg
     4861 cgcccgggcg cttcgatcga aactgtaggc ccgaaaccac gaggagaacg gccattcccc
     4921 caccgtcgcc acctccaccg gccggccgtc gggcgcgtcg gtcggttcgt tgccgaggcg
     4981 gtggacggcg agccgcggtt cgtagccgtt cggggcgagc cgacgcgcca attccagggc
     5041 gacgcgcccg ccgctggtgg ccaggtcgcg cgccgcgatg ccgatggggg aaccaatcat
     5101 gggggatctc cgttgttcat tcgacgcggc cggccgcgtc caagacttcg tcgtagcagg
     5161 cgcgcaggct tcggaaatgc gcctcccaat cgttcgcgcg caccgcgggc gccagcgcgg
     5221 cgcccagggc ccgtcgaagg agcggatcct ccaccaacgc ggccagccgg gcggacaatt
     5281 cggaaacttc cggcgcttcc gccaaccacg cgttccacgc cccgggccag aactccgagg
     5341 cgccgacgcg acggctcgtc aacacgggaa cgccgcaggc catggcctcc tgcacgctca
     5401 tgccgaattc ttcgaagggc gccggatgaa cgtagacgtc cagcgcgtgg tagagggcct
     5461 ccacgtgaac ctggggggtt tcgaattgca cccggtcgcc cagatccagg gaccgcatca
     5521 aattcaaata gggggccgga cgggtttctt ttcccaccac gaccgcgcgc acccggcgac
     5581 gaaccgccgg cggcaatccg gccagggccc gcagaaacaa gggcagtccc cgcttttcaa
     5641 aatcccccga ggtgacgagt cccaccaccg tttcctcctc gcccacggcc cagcggcgtc
     5701 gttgggcctc ccgccaaagg gttcggtcct ccggacggaa ccgggccgga tcaaaccccg
     5761 gatacacgac gcggacccga tccggcggaa cgccgaaccg ctgttccacg tcgtttttca
     5821 tgaggcggga gttggcgatc aagaggcgga accgccggcc ccgcaattga cgctcgtgca
     5881 tccatcccgc gccggacccg gcgggcaac
//
LOCUS       DAOQLS010000031         5878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5923_length_5878_cov_4.486519, whole genome shotgun sequence.
ACCESSION   DAOQLS010000031 DAOQLS010000000
VERSION     DAOQLS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_5878_cov_4.486519"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PK362_01105"
     CDS             complement(<1..649)
                     /locus_tag="PK362_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC73472.1"
                     /translation="MIQKSIRGLAAIALMLGALPAAAEKKIIKIDGSSTVYPITEAVA
                     EEFMKTHPDIQVTVGISGTGGGFKKFCRGETDISDASRPILRKEMDACLDAAVQYYEL
                     PVAFDALTVMINPKNTWAATMTVAELKKIWEPAAQGQVTKWNQVNPTWPDKPLKLYGA
                     GSDSGTFDYFTEAVNGKAKSSRGDYTASEDDNVLVQGIASDEGALGYFGLAYYVEN"
     gene            complement(831..2009)
                     /locus_tag="PK362_01110"
     CDS             complement(831..2009)
                     /locus_tag="PK362_01110"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNC73473.1"
                     /translation="MNQGNRTTRRALGLAGVLALGALVAPAARAGTEISFADWVTKVT
                     VKGDMRLRSDSEIIRQGVDRNRQRMRIRLQGEIPVSPTLKTVFGIASGEGEQTSTNQS
                     FKALSSQKALWIDLAYLEYKPLETFRITAGRMKNPLWLAYSDDAVWDGDFNPEGLAQN
                     LKLSLFGSGRVFLNGLQGVADEDSASPKDQFYFTEQLGVIWPLPADSRITVAGAVTEW
                     VNESSATRKQLGSAATFQQVKTQDGNRRLGTGLANEFRVMELTAEYLIPVFDRPLSVQ
                     GTYVKNLASLDVYTDKADRGNQVGLIYGKASGKGKWEAAYFHKQLATDATVADVADSD
                     FGVGGTNRRGHLAWFSYAPADYAVAQVKFFNTKLNDTAFNSGKPKDVNRLQIDFSVKF
                     "
     gene            complement(2170..2346)
                     /locus_tag="PK362_01115"
     CDS             complement(2170..2346)
                     /locus_tag="PK362_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73474.1"
                     /translation="MKPRNPQLGRLLARIQSRALQDGVIDHWNRCRRLPPGFLNLPVF
                     SSNHRGSSRRELAA"
     gene            complement(2389..2616)
                     /locus_tag="PK362_01120"
     CDS             complement(2389..2616)
                     /locus_tag="PK362_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73475.1"
                     /translation="MAWAPDFGYDESNKNRRRPNMSEQKIEILRAALRAAEGQLKEIL
                     LGLETKDLDDQAVSGQLREIERGLRRLARAA"
     gene            2779..4731
                     /locus_tag="PK362_01125"
     CDS             2779..4731
                     /locus_tag="PK362_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73476.1"
                     /translation="MTKSKGLSLVESMIAMVVVGIIAIVLIRLMQGGGKSIVSSQNQF
                     EAAQLLDYIQAKFQIARFEDVLAVDSGNKNGVTAGNFSPSQYWVRWSSSPIQGMLLDI
                     ETRVKEAGFPRFKVEVKFIRRDSADNYANSSVVDFIAFEPLATCSVAAGCVDKYNPEI
                     RFQSLIANAPTEFFYDVDGAGRTEVPDTRMKTLDIYLCRTAACNASDPGTVMSREGVM
                     LSKEKLSGRAQENYVKDLKLLMTFPVDSSRYYRDRAEIYYGYASGAAHTAGSTIGAGP
                     LTNQFFRRDNYAKTSSWSWQLRPGDKTNGSLIPKVAGYSERDAIISLWTSTVPYPPVA
                     APNYTWTVTADNGYFDNPWSGAFADAVYLEGVHGFVMRAKKGPVGNEMFSPWRIVYMN
                     VDITPPVARNADILPLPGSNVPNRSPYLKLFLRDEDSGNLIPTHSSPPRHLPGVSGIA
                     EESLSIATMTSPAPAAVGNINYMRHLYTPVTPAVSGVVSASGTLVAIDTTTLLPYVFP
                     DNQDIYLRAEFGDKTGQKTIKTWTIHIDDMNFDDGTSPDVVVPSTTPPTLAGAPLVLS
                     VSDPESGVDWGSFELFDVDHGAVLLSSVTSPSLGLCLNPATNSIVYSTSSFTPGTHHL
                     RTTVSNWSRGGGTNATTVRNFDMVIQ"
     gene            4728..5510
                     /locus_tag="PK362_01130"
     CDS             4728..5510
                     /locus_tag="PK362_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73477.1"
                     /translation="MTAGNRGMSLPEVLIASVIFAVVLLGTVVFVKKSSEGLFHLLGR
                     ISTEERLRMVNQDVLRDLGEMNEVLEVDVSSITFVLDSHRFPGYNPNALVRGLAARYQ
                     PDQDGDAVFLATAPGCLRYMGNDLDDDDDDNDGRIDVQCRYKFSNGQIQRWFNYNEAG
                     WVESPLVRGGPGFVVNEFSLVPAGAKSIEAYAAWDTNADGEVSQSEIAVQGGNPLFID
                     TRPELAAVATLRVDMEIQKGKEKIKTSTQIIPPLLVAKRKAP"
     gene            5507..>5878
                     /locus_tag="PK362_01135"
     CDS             5507..>5878
                     /locus_tag="PK362_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73478.1"
                     /translation="MRRLRGTPGGMVTLPVILVLFFLIPLFLMLMSRLRLHNMHNYAD
                     RTIRAKRLISTNFISDFMRQFSEDFRQDHYMRFWLDRSNIFAADPAVYNFTGGRTKIK
                     INNDIVGALDETTVRTNHVFVM"
BASE COUNT         1119 a         1717 c         1778 g         1264 t
ORIGIN      
        1 tgttttccac gtaataggcc aggccgaaat agccgagggc gccttcatcg gaggcgatcc
       61 cctggaccag gacgttgtcg tcttccgagg ccgtgtaatc gccccggctg gatttggctt
      121 ttccgttgac ggcttcggtg aagtagtcga aggtgccgga atcggacccc gcgccgtaaa
      181 gtttgagggg tttgtcgggc caggtcggat tgacctggtt ccatttcgtc acctgtccct
      241 gggccgccgg ctcccagatt ttcttcagct cggcgacggt catggtggcc gcccaggtgt
      301 ttttggggtt gatcatgacg gtcaaggcgt caaaggcgac gggcagttcg tagtactgga
      361 cggcggcgtc caggcaggcg tccatttcct tgcggagaat gggccgggag gcgtccgaga
      421 tgtccgtttc gccgcggcag aatttcttaa acccgccccc ggtgccggaa atccccaccg
      481 tcacttgaat gtcggggtgg gttttcatga attcctccgc caccgcttcg gtgatcgggt
      541 agacggtgct ggaaccgtcg atcttaatga ttttcttttc ggccgccgcg ggcagggccc
      601 cgagcatcag ggcgatggcc gccaggccgc gaatcgattt ctggatcatt gaatcctccg
      661 tggtcattcc gatcttacga accgactgtg accgttgaat gacgttcgtg taaccgtgtt
      721 gtaatcaaat cggtggaggg agcgccgagc ggaaaatgag ggaaaccacc cggacgctcc
      781 ctccgaattg attcacgaca atccctcgtt gtcgaagagc gtggcgatcc ttagaacttg
      841 accgagaagt cgatctgcag tcggttcacg tccttgggtt tgccgctgtt gaaggccgtg
      901 tcgttcagct tcgtgttgaa gaacttcacc tgggccaccg cgtagtccgc gggggcgtag
      961 gagaaccagg ccaggtgacc gcgacggttg gtgccgccca cgccgaagtc cgaatcggcc
     1021 acatcggcca cggtggcgtc ggtggccagc tgtttgtgga aataggccgc ttcccatttg
     1081 cccttgccgc tggctttgcc gtagatcaac cccacctgat tgcctcggtc cgccttgtcg
     1141 gtgtaaacgt ccaaggaagc gaggtttttg acgtaagtcc cctggaccga gaggggacgg
     1201 tcaaagacgg ggatgaggta ctcggccgtc aattccatca cgcggaactc gttggccagg
     1261 cccgtgccca gccggcggtt gccgtcttgg gttttgacct gttggaaagt cgcggcggag
     1321 ccgagctgtt tgcgggtcgc cgaggattcg ttgacccatt cggtgaccgc tcccgccacg
     1381 gtgatgcggg agtccgcggg caagggccag atgacgccca attgttcggt gaaatagaac
     1441 tggtctttcg gggaggcgct gtcctcatcg gccacgccct ggagcccgtt caagaacacc
     1501 cggccggagc cgaagagcga caacttcagg ttttgcgcca acccttcggg gttgaagtcg
     1561 ccgtcccaga ccgcgtcgtc ggaataggcg agccagaggg ggttcttcat ccggccggcg
     1621 gtgatccgga aggtctccag gggcttgtat tccaggtagg ccaagtcgat ccaaagggct
     1681 ttctggctcg acagcgcttt gaagctttga ttcgtcgacg tctgttcgcc ttcgccgctg
     1741 gcgatgccga aaacggtctt caacgtgggg ctgaccggaa tttcgccctg caggcggatg
     1801 cgcatccgtt ggcggttgcg gtccacgccc tggcgaataa tttcgctgtc ggagcgcagg
     1861 cgcatgtcgc ctttcacggt gactttggtg acccaatcgg cgaagctgat ttccgtcccg
     1921 gcgcgggccg cgggagcgac cagggcgccc agggcgagaa cgccggccag gccgagggcc
     1981 cggcgggtgg ttcgattgcc ttgattcatt tgattcctcc attttaatgt cgcgccgaag
     2041 cgctcgaaat tcatcggtgt gaacggccgc actttaattc aaattccatt gaaggggaat
     2101 tgcggccagg tcaaatcccc gtgacggcgg cgtgacgacc ggtcggccgc gacgctccga
     2161 cggaataatc taggcggcga gttcccgccg ggaggacccc cggtggttgg aagaaaagac
     2221 cgggaggttg agaaaccccg ggggaagccg gcggcaacgg ttccagtggt caatcacgcc
     2281 gtcctggagg gcccgggatt gaatccgggc cagcaggcga cccagctggg ggttccgggg
     2341 tttcatttgc ccgaggaggt ctccggccgt gcggggggtg cccacgatct acgccgcccg
     2401 ggccagccgc cgcaggcccc gctcaatttc ccgcagttgg ccgctgacgg cttggtcgtc
     2461 caggtccttg gtttcgaggc ccaggaggat ttccttcaat tgaccctccg ccgcccgcaa
     2521 cgcggcccgg aggatttcga tcttttgttc gctcatgttc ggtctccttc gatttttatt
     2581 gctttcatcg tagccgaagt ccggcgccca agccattgtg gggaggtaaa accggtgtga
     2641 agcccgtgtg acgttttcgg cgggggaaaa tcgcccggtt tcgccatttt gttgcatggg
     2701 aattaagagg aaaggacaca ataaaaggag ttgaagtcgg gggtttgagg ggttaaccta
     2761 agagccggag gggtctccgt gacgaaaagc aagggcctgt ccctggtcga atccatgatc
     2821 gccatggtgg tggtgggaat catcgccatt gtgttgatcc gcctcatgca gggcgggggg
     2881 aaaagcattg tttcctccca gaaccagttc gaggcggccc agttgctcga ctacatccaa
     2941 gccaagtttc aaatcgcccg attcgaagac gtccttgccg tcgattcggg aaataaaaac
     3001 ggcgtcacgg ccggaaattt cagtccctcc caatattggg tccgctggtc gtcctcgccc
     3061 attcaaggca tgcttttgga catcgaaaca agggttaaag aagcgggctt tccccgtttt
     3121 aaggtggagg tcaaattcat tcggcgggat tcggcggaca attacgccaa cagcagcgtc
     3181 gtggatttca tcgccttcga acccctggcg acctgcagcg tggcggccgg ttgcgtggac
     3241 aagtacaacc ccgaaatacg ttttcaatcc ctcatcgcca acgctccgac ggagtttttt
     3301 tacgatgtgg acggcgccgg ccggacggaa gttcccgaca cccggatgaa aaccctggac
     3361 atctatttgt gtcgtaccgc ggcctgcaat gccagcgacc ccggaacggt gatgtcccgg
     3421 gaaggcgtga tgttgtccaa ggaaaaatta tcggggcggg cccaggaaaa ttacgtgaaa
     3481 gacctcaaat tgcttatgac cttcccggtg gattcctccc gctattaccg ggaccgggcc
     3541 gaaatttatt acggctacgc cagcggagcc gcccacaccg ccggttcaac gatcggggcc
     3601 ggtcccctca ccaatcaatt tttccgccgg gacaattacg ccaaaacatc gtcctggagt
     3661 tggcaattac gcccgggcga caagaccaac gggagcttga tccccaaggt ggcgggctat
     3721 tccgaacggg acgcgattat ttcgctatgg acctccacgg tgccctaccc cccggtggcg
     3781 gcgcccaatt acacctggac ggtcacggcc gacaacggct atttcgacaa tccctggtcg
     3841 ggggccttcg ccgacgcggt ttatttggaa ggcgtccatg gtttcgtcat gcgggccaag
     3901 aaagggccgg tgggcaacga aatgttttcc ccctggcgga tcgtctacat gaacgtggac
     3961 atcacgccgc ccgtcgcccg gaacgcggac atcctccccc tgccggggtc caacgtcccg
     4021 aaccggtctc cgtatctcaa gcttttcctg cgagacgagg acagcggcaa tttgattccg
     4081 acccattcat cgccgccccg ccatcttccc ggcgtgtcgg gcatcgccga agaatccctt
     4141 tccatcgcca ccatgacctc tccggccccc gcggccgtgg gaaacattaa ttatatgcgc
     4201 cacctctaca cccccgtgac tcccgcggtt tcgggggtgg tttcggccag cgggacgttg
     4261 gtcgcgatcg ataccacgac cctgctgcca tacgtttttc cggacaatca ggacatctat
     4321 cttcgggctg aatttggcga taaaaccggc caaaaaacga ttaaaacctg gaccattcac
     4381 attgacgaca tgaattttga cgacggaacc tccccggacg tggtggtccc ctccacgacg
     4441 ccgccgactt tggcgggcgc tccgctggtc ctcagcgtgt ccgaccccga aagcggcgtg
     4501 gattggggat cgtttgagtt gttcgatgtg gatcacggcg ccgttttgct gagcagcgtg
     4561 acatcgccct ccctgggatt gtgcctgaac ccggcgacca attccattgt ttattccacg
     4621 agcagtttca cccccgggac gcaccatctg cgaaccacgg tttccaattg gtcccggggc
     4681 gggggcacca acgccacgac ggttcgaaac ttcgatatgg tcattcaatg acggcgggga
     4741 atcggggcat gtcgttgccc gaggtgttga tcgcttcggt gattttcgcc gtcgttttgc
     4801 ttgggacggt ggtgtttgtt aaaaaaagct ccgaagggtt gttccacctc ctgggtcgga
     4861 tttccacgga agagcgcctt cgaatggtga atcaggacgt ccttcgggat ctgggggaaa
     4921 tgaacgaagt gttggaagtc gatgtcagca gcatcacctt tgttttggat tcccaccggt
     4981 tcccggggta caaccccaac gcgctcgttc gggggttggc cgcccgttac caaccggacc
     5041 aggacgggga cgcggttttt ctggccacgg cccccggctg tttgcgttac atggggaacg
     5101 atctggacga tgacgacgac gacaacgacg gtcgcatcga cgtccagtgc cggtacaaat
     5161 tcagcaacgg acaaattcag cgttggttca attacaacga agccggctgg gtcgaatcgc
     5221 cgctggttcg cggcggtccc ggattcgtgg tgaatgaatt ttcgctggtc ccggcggggg
     5281 ccaaatcgat cgaggcctac gccgcttggg acaccaacgc ggacggcgag gtcagccaat
     5341 ccgagatcgc cgtccagggc gggaacccgc tgttcatcga cacccgcccg gagctcgccg
     5401 ccgtggccac cctccgggtc gatatggaga ttcaaaaagg gaaggaaaaa ataaaaacct
     5461 ccacccaaat cattcccccg ttgctggtgg ccaaaaggaa agccccgtga ggcgccttcg
     5521 ggggacgccc gggggcatgg tgaccctgcc ggtcatcctt gttttgtttt tcctcatccc
     5581 ccttttcctg atgttgatgt cccgccttcg cctgcacaac atgcacaatt acgccgaccg
     5641 caccattcgg gccaaacgat tgatttccac caattttatc tccgatttca tgcggcaatt
     5701 ttcggaggat ttccgccagg accattacat gcgtttttgg ttggaccggt ccaacatttt
     5761 cgcggcggac ccggccgttt ataatttcac cggcggtcga acgaaaatta agatcaacaa
     5821 cgacatcgtc ggcgccctgg acgaaaccac cgtgcgcacg aaccacgtgt ttgtcatg
//
LOCUS       DAOQLS010000032         5854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5957_length_5854_cov_4.258838, whole genome shotgun sequence.
ACCESSION   DAOQLS010000032 DAOQLS010000000
VERSION     DAOQLS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5957_length_5854_cov_4.258838"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PK362_01140"
     CDS             <1..359
                     /locus_tag="PK362_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNC73479.1"
                     /translation="ESGGGHGETAAPAHGAETGVAYPLKTTVVNLASDSDHRFAKVSI
                     TLEGQDAATIDRLKELDHQVYDSLIEILGNVRAQDVATDEGKERLKDSIRERLNRLVP
                     DNAGVKSVYLTEFLVQ"
     gene            356..1252
                     /locus_tag="PK362_01145"
     CDS             356..1252
                     /locus_tag="PK362_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HNC73480.1"
                     /translation="MTESSGVQRFDPRRGGGLVKATREQLARIHKEFTLKTASTFPDR
                     LGLPLRVTFRDLATIGWDQCQSAAGASAAVLPFALTPGDGFGFLRLSADGARAFVDAS
                     FGGPGRSGPGGAPLTDVERRSAVWPLRLFLTEWNALWEAFGECAFNADEIGGADAHFS
                     SAAGEPHVVAGFDWSIGETTGRLEAALPLSFARPFLGELEKARGESNALWTDPKMRPV
                     LRQRAEGVSLPLRVCLVESDIPVRDMADLEVGDVVRLGSVNQSAVVKIGEISLFHAKA
                     GVSNGRLAVRVLALAGNGRSAS"
     gene            1249..1611
                     /gene="fliN"
                     /locus_tag="PK362_01150"
     CDS             1249..1611
                     /gene="fliN"
                     /locus_tag="PK362_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNC73481.1"
                     /translation="MTESEKPAENPAPGATPEARPVQLGEVPKSGPVEPLAVNIAPLM
                     DVPLKVSVLLGETRMSLGDLLRLGTGSIIELSRSAGEPIDVLVNDRLYARGEIVAVGE
                     DFGVRVVELVAAPKTSAA"
     gene            1616..2047
                     /locus_tag="PK362_01155"
     CDS             1616..2047
                     /locus_tag="PK362_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNC73482.1"
                     /translation="MSPSLRRAFFGGATLAGLSPALAAAEADWLSTPGAPETPFPWIR
                     LGFLVALTIVAVVSARRWGRARVRNVEAPDELSLLGKLPLGPKNGIAFIAVGSRRVLV
                     GFGEQGVRPLARWEEKAGPAPAGAWTPVLRGALPNALFHES"
     gene            2037..2771
                     /gene="fliP"
                     /locus_tag="PK362_01160"
     CDS             2037..2771
                     /gene="fliP"
                     /locus_tag="PK362_01160"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNC73483.1"
                     /translation="MKAKALPWVLPILLAAGPVLAAAGPADGLLRSLGEPKRLSSAME
                     TVVVLTILSLIPTVLILTTCFLRFSLVLGFARQALSLQQTPPNHVLISLAMVLTFFVM
                     GSTLKTINDTAVQPYRQGKLAFEKALEIGAQPLREFLLKQTRRRELSLAVRLARLEPK
                     TEADVPFHVLTTAYVLSELKTAFQMGFLLFIPFLVIDLGVSSVLMSLGMMMVPPNMVA
                     LPLKLLLFVMVDGWSLLVQGLVESVR"
     gene            2776..3045
                     /gene="fliQ"
                     /locus_tag="PK362_01165"
     CDS             2776..3045
                     /gene="fliQ"
                     /locus_tag="PK362_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNC73484.1"
                     /translation="MNADVVASLLRDAFWTVLLAAGPVLAVALAAGIVVGLFQAATQI
                     HEMTLVFVPKLLLVALVTAVLGHWQWAALVRFAERMITALPQAAR"
     gene            3050..3811
                     /locus_tag="PK362_01170"
     CDS             3050..3811
                     /locus_tag="PK362_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNC73485.1"
                     /translation="MTFPDPVKFLFVFARILGLFAVAPPWAGRGVPVARLGAALGVSV
                     LVVSWAGGGPPAATDAWGVGLGVISETGVGAALGGLLALFFSAYQTAGHLIGNQMGFG
                     WAGFYDPRTNESVDDLAELYHRLALLLFFILDGPWRLLKLTVWSFEKIAPGQALWTGR
                     LAGVWVESVGRSMALAAAAAAPTVGALWLASLLVGFLVRAAPQMNLAAVDFPARALIG
                     FASLWVGGAALIRFFPGTAATAFDQLSLFLKSLGG"
     gene            3815..4876
                     /locus_tag="PK362_01175"
     CDS             3815..4876
                     /locus_tag="PK362_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNC73486.1"
                     /translation="MAPGSDQKTEKPSPHKLQKARRQGQIAQSREIPAVAALVGAAAV
                     LIAQGGQWASRWGGLWRETVALAGKTAGESVPLQAAAILALKGGAALVLPVLAAAVLS
                     ALALGAVQTRGLFAPSLLKFDWSRSNPLSGAGRLLSPRNGADALKMFLKAAGFALIAG
                     RAVWSLAVQSPAELARGLPAMAAQVGRGFSEVLWDAILFFSALAAWDYYRQHRRWEAD
                     LRMSKQELKEEHREMEGNPQIKSRLKSLRKKWASRRMMSQVPKADVVVTNPTHLAVAL
                     RYRPRTSAPEVVAKGADLVAQRIRELARAHGVPLLENKPLAQLLYQKVDVGSPVPVAL
                     YQAVAEVLAYVYRLKNPRR"
     gene            4880..>5854
                     /locus_tag="PK362_01180"
     CDS             4880..>5854
                     /locus_tag="PK362_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNC73487.1"
                     /translation="MAEPMIQKRTLDVLTAFGILGVLATMILPLPTWLLSFLLILNLA
                     MSVTVLLVVLNIREILDFSIFPSVLLGLTLFRLALNVASTKRILMSGDAGAVIEAFGQ
                     IVVRGDAVVGFVVFLILVVVQFMVITKGAERVSEVAARFTLDALPGKQMAIDAELNAG
                     LIDEKTATQRRQNIQREADFYGAMDGASKFVRGDAIAAIMIVGINILGGAVLGFWRRH
                     MELADVLRTYTVLSIGDGLVHQIPALIVSTASGVLVTRSATQSPLGQEWARQIAANPR
                     ALGMAGFTMSLLGVTGLFTGLPVVPFLVVGGTLASMAWVARPGAAPSAA"
BASE COUNT          879 a         1828 c         1957 g         1190 t
ORIGIN      
        1 cggagagcgg cggcggccac ggcgaaacgg cggccccggc ccacggcgcg gaaacgggcg
       61 tggcgtaccc gctcaaaacg acggtcgtga atttggccag cgactccgat catcggtttg
      121 ccaaggtgtc catcaccctg gaggggcagg acgcggccac catcgaccgg ttgaaggaat
      181 tggaccacca agtctacgat tccctgatcg agatcctggg gaacgtgcgc gcccaggacg
      241 tggccaccga tgagggcaag gagcggctca aagattcgat ccgggaacgc ctcaatcggc
      301 tcgtgcccga caacgccggc gtcaaaagcg tctatctcac ggagttcctg gtccagtgac
      361 cgaatcgtcg ggggtccagc gctttgatcc gcgtcggggc ggcgggttgg tcaaggccac
      421 ccgggagcaa ttggcgcgga tccacaagga attcaccctc aaaaccgcgt cgacctttcc
      481 cgaccgattg ggcctcccgt tgcgggtgac ctttcgggat ttggccacca tcggctggga
      541 ccaatgccaa tccgcggcgg gggcgtcggc cgccgttctg cccttcgctc tgacgcccgg
      601 agacggattc gggtttttgc ggttgtccgc ggacggggcc cgcgctttcg tcgacgcgtc
      661 cttcgggggg ccgggccggt ccggtcccgg cggggcgcct ttgaccgacg tcgagcggcg
      721 atccgcggtt tggccgcttc gcctttttct gaccgagtgg aacgcgctgt gggaagcctt
      781 cggcgagtgc gccttcaacg ccgacgaaat cggcggggcg gacgcgcact tttcaagcgc
      841 cgccggggag ccccacgtgg tggccggatt tgattggtcc atcggggaga ccaccgggcg
      901 gctcgaagcg gccttgcccc tgtccttcgc gaggcctttt ctcggtgaac tggaaaaggc
      961 ccggggggag tccaacgcgc tctggaccga tcccaaaatg cgaccggtcc tccgccagcg
     1021 ggcggagggc gtttcccttc ccctccgggt gtgtctggtg gaatcggaca ttccggtgcg
     1081 ggacatggcg gatttggaag tgggcgacgt cgttcgtctg ggcagcgtga accagagcgc
     1141 cgtcgttaaa atcggtgaaa tttcattgtt ccacgccaaa gccggcgtgt ccaacgggcg
     1201 gctggccgtt cgggtgctgg ctctggccgg aaacggaagg agcgcgtcgt gacggaatcg
     1261 gagaaacccg ctgaaaatcc cgcccccggg gcgaccccgg aggcccgtcc ggtgcagttg
     1321 ggcgaggttc ccaaaagcgg gccggtggag cccctggcgg tgaacatcgc ccccctgatg
     1381 gatgtgccgc ttaaagtgag cgttttgttg ggcgaaaccc ggatgtccct gggggatttg
     1441 cttcggctcg ggacagggtc catcatcgaa ttgtcccgaa gcgccgggga acccatcgac
     1501 gtcctcgtca acgaccgcct ctacgcccgg ggcgagatcg ttgcggtcgg tgaagatttc
     1561 ggcgtgcgcg tcgtggagtt ggtggccgcg ccgaagacgt cggcggcctg aattcgtgtc
     1621 cccgtccctg cggcgcgcat tcttcggggg ggccacgctg gcgggtctgt cccccgcttt
     1681 ggcggcggcc gaggcggatt ggctttccac ccccggggcg cccgaaacgc ctttcccgtg
     1741 gattcgcctc ggtttcttgg tggcgctgac gatcgtggcg gtggtgagcg cccggcgatg
     1801 ggggcgggcg cgggtccgga acgttgaggc cccggacgaa ttgtctcttt tggggaaatt
     1861 gcccctcggc cccaaaaacg ggatcgcctt tatcgccgtg gggtcccggc gcgttctcgt
     1921 gggtttcggg gaacaagggg tgcgtcccct cgctcgctgg gaggaaaagg cgggcccggc
     1981 gccggccggg gcctggaccc cggtcttacg cggcgccctt ccgaacgcgc ttttccatga
     2041 aagctaaagc ccttccctgg gtcctgccga tccttctggc ggcgggtccg gtcttggcgg
     2101 cggccgggcc ggcggacggc cttctccgtt ccctgggcga acccaagcga ctgtcctcgg
     2161 ccatggagac cgtcgtcgta ttgacgatcc tttccttgat tcccacggtg ttgattctca
     2221 ccacgtgctt tctccgcttc tcgctcgttt tgggattcgc ccggcaagcc ctcagcctcc
     2281 agcaaacccc gcccaaccat gtcttgatca gtctcgccat ggtcctgacg tttttcgtca
     2341 tgggatcgac gctaaaaacc atcaacgaca ccgcggtgca accttaccgc cagggaaagc
     2401 tcgcctttga gaaggctctg gaaatagggg cccagccgct ccgggagttt ttattgaaac
     2461 aaacccgacg tcgggaactg tcgctggcgg tccgcctggc gcgcctggaa cccaaaaccg
     2521 aagcggacgt cccctttcac gtgttgacca cggcctacgt gctgagcgaa ctcaaaaccg
     2581 cttttcaaat ggggtttctc cttttcatcc cctttctggt cattgatttg ggggtgtcca
     2641 gcgtgttgat gtctttgggg atgatgatgg ttccgcccaa catggtcgcc cttcctttga
     2701 aactcctgct ctttgtgatg gtcgacggat ggtccctgtt ggtgcagggg cttgtcgaga
     2761 gcgttcggtg atccggtgaa cgccgacgtc gtcgcctccc tcctgcggga cgccttttgg
     2821 accgtgctcc tggcggccgg gccggtgttg gccgtggccc tggccgcggg cattgtggtg
     2881 ggtctgttcc aagcggcgac tcaaattcac gagatgacgc tggtgttcgt ccccaaattg
     2941 ttgctggtgg ccttggtgac cgccgttctg ggacactggc agtgggccgc cctggttcgt
     3001 ttcgccgagc gcatgatcac cgcccttccc caagccgccc gctaagaccg tgacgtttcc
     3061 cgatccggtg aagttcctgt ttgttttcgc ccgaattctc gggctctttg ccgtggcacc
     3121 gccctgggcc gggcgcggcg ttcccgtggc ccgtttgggc gccgccctgg gggtgagcgt
     3181 attggtcgtg tcctgggccg gcggggggcc gccggcggcg accgacgcct ggggcgtggg
     3241 actcggcgta atatcggaaa ccggcgtcgg ggcggccctg ggggggctac tggcgctttt
     3301 cttttcggcc taccaaaccg ccgggcattt gatcgggaat cagatgggat tcggttgggc
     3361 gggattttac gatccccgga cgaacgaatc cgtggacgac ctggcggaac tctaccaccg
     3421 actggcgctc ctgctctttt ttattttgga cgggccctgg cggctgttga aattgacggt
     3481 ctggagcttt gaaaaaatag cgccggggca ggccctttgg acgggtcggc ttgccggcgt
     3541 gtgggtggaa agcgtgggcc gttccatggc cctggcggcg gccgccgcgg caccaacggt
     3601 gggggccctt tggttggctt cgctgttggt gggtttcctc gtccgggcgg cgccccagat
     3661 gaatctggcg gccgtcgatt ttcccgcccg ggccttgatc gggttcgcct ccctgtgggt
     3721 ggggggcgcg gctttgatcc gtttttttcc gggaacggcc gccaccgctt ttgatcaatt
     3781 gtcccttttt ctaaaaagcc tgggggggta atccgtggcc cccgggtccg atcagaaaac
     3841 cgaaaagccc tctccccata aacttcaaaa ggcccgacgg caggggcaaa tcgcccagag
     3901 ccgggaaatc ccggccgtgg ccgctttggt cggggcggcg gccgtgttga tcgcgcaggg
     3961 agggcaatgg gcctcccggt ggggggggct gtggagggag accgtggccc tggcgggaaa
     4021 aaccgcgggg gaaagcgtgc cgctccaggc cgcggcgatt ttggccctca aaggcggggc
     4081 cgccctcgtc cttcccgtgt tggccgcggc cgttttaagc gccctggcgc tgggcgccgt
     4141 tcaaacccgg ggcctgttcg ccccgtccct cctgaaattc gactggtccc gctccaatcc
     4201 gttgtccggc gcgggccgcc tgctcagccc ccgaaacggc gcggacgcgc tgaaaatgtt
     4261 tttgaaagcc gcggggtttg ccttgatcgc gggccgggcg gtgtggtccc tggcggtgca
     4321 atcgcctgcg gaactcgccc ggggcctgcc cgccatggcc gcccaggtgg ggaggggttt
     4381 ttcggaggtt ctgtgggacg cgatcctctt tttttcggcc ttggcggctt gggattatta
     4441 ccgccaacac cgccgctggg aagccgacct ccgcatgagc aagcaggaat taaaggaaga
     4501 gcaccgggaa atggaaggga acccgcaaat aaaatcccgc ttgaaaagtt tgcgcaaaaa
     4561 atgggcctcc cgccgcatga tgagccaggt gccaaaggcc gacgtggtgg tcaccaatcc
     4621 aacccacctg gccgtggccc tgcgttatcg accccgaacc agcgcgccgg aagtcgtggc
     4681 caaaggggcc gatttggtgg ctcaacgaat tcgggaactc gcccgggccc acggggtgcc
     4741 gcttctcgaa aacaagccct tggcacaact cttgtatcag aaagtggatg tggggagccc
     4801 cgttcccgtg gccctctacc aggcggtggc cgaggtcctg gcctacgtgt atcgcctgaa
     4861 gaacccccgg aggtaaggcg tggccgaacc gatgattcaa aagcgcacct tggacgtcct
     4921 gaccgccttc ggcattctgg gcgttctggc caccatgatt ttgcccctgc cgacctggct
     4981 gctcagtttc ttgctgatcc tcaatctggc catgtccgtc accgtgctgc tggtcgtgtt
     5041 gaacatccgt gaaatcctcg atttctccat cttcccctcg gtcctcctgg gcctgacgct
     5101 ctttcggctc gcgttgaacg tcgcctccac caaacgcatt ttaatgtccg gggacgccgg
     5161 cgccgtcatc gaagccttcg gccaaatcgt ggtccggggg gacgcggtgg tcgggttcgt
     5221 cgtgtttctc attttggtgg tcgtgcagtt tatggtcatc accaagggcg ccgagcgggt
     5281 ttcggaagtc gcggcccgtt tcaccctcga cgctcttccc gggaaacaaa tggccatcga
     5341 cgccgagttg aacgccgggt tgatcgacga aaaaacggcc acccagcggc gacaaaacat
     5401 ccaacgggaa gccgatttct acggcgccat ggacggcgcc tccaaattcg tccggggcga
     5461 cgccatcgcg gccattatga tcgtggggat caatattttg gggggcgccg tgctgggttt
     5521 ctggcgtcgc catatggagc tggccgacgt gttgcgaacc tacacggtcc tctccatcgg
     5581 cgacgggctg gtccatcaga ttccggcgct catcgtgtcc accgcgtccg gggtcctggt
     5641 gacccggtcg gcgacccaat cgcctctcgg ccaggaatgg gcccggcaaa tagccgccaa
     5701 cccccgcgcc ctgggcatgg cggggttcac catgtcgctc ctgggcgtca cgggattgtt
     5761 cacggggctg ccggtcgttc ctttcctcgt ggtgggcggc accctggcct ccatggcctg
     5821 ggtggcccgg cccggcgcgg cgccgtcggc ggcg
//
LOCUS       DAOQLS010000033         5853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_5964_length_5853_cov_3.191100, whole genome shotgun sequence.
ACCESSION   DAOQLS010000033 DAOQLS010000000
VERSION     DAOQLS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5964_length_5853_cov_3.191100"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK362_01185"
     CDS             <1..403
                     /locus_tag="PK362_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73488.1"
                     /translation="IDVEIHVFPEGEAISAFKSQPWNLGLTTLPSPIAHVAFPMGLFF
                     YSNSFFSMHHDPVFDRMYEKATSIMDPAESEKAFQELERKVYDDALGVFLYRRIKTYA
                     VSKRVRFSPYLTGMPHFVDAVSLVKEEANKK"
     gene            400..819
                     /locus_tag="PK362_01190"
     CDS             400..819
                     /locus_tag="PK362_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73489.1"
                     /translation="MIPAISPTAIPILLVVAGGTHFAQIGAMHWYAYEWARRNPLPEG
                     PGVLFRALRSGLIFMVVGTGLLVIFNAGRIAAGGGLAVSLCALLSLFAGYRLGLQWFG
                     MGKEMDHAGNRGARAVLYATLVLQFLIYAGVFLALVR"
     gene            833..1759
                     /locus_tag="PK362_01195"
     CDS             833..1759
                     /locus_tag="PK362_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNC73490.1"
                     /translation="MIQTSLWAKVRRWSGALWVVALVVSARAAQPPLNMCATTLLDGG
                     PPPGFYYLNYAIFTEGKKFRDGDGNEAANMGKLNTFSQLHQFYYIADFNVLGAHPALD
                     VLVPVAALTARGGTAGPFSLNSNTAGLGDLVVGPALHWDGRTLFGKPVFHRVELDLTI
                     PTGKYSKDQLFNPGSNLHTIEAYYSLVMILPPKWETSWRFFYAHHSENKDHLFGRLKP
                     GAVVHANYALSRQVGEKWRVGAAGYYLQQLREDELNGAKQVKSKERVVSIGPAVHYGV
                     PGWSLVVSHPVEFAVRNRFQGSRTTLQLIHRF"
     gene            1856..3400
                     /locus_tag="PK362_01200"
     CDS             1856..3400
                     /locus_tag="PK362_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC73491.1"
                     /translation="MKKAARWFMPEGSRRWLPGLAVLLVLVALGRARESPSTVVVCND
                     TQDPRTMDPFLELTEKNHVLLQQMMEGLVRFDPEGKVIPCLAESWEQVDLLRLRFHLR
                     KDVEFHNGEPFTAEAVRFSAQTFSDPKGVYPGKGFVSSIDRVEIVDPLTVDVVTHFPD
                     GILLRRFAGLIFMVPPDYYKKVGSQGFSDRPVGTGPFRFVRHVPGKEIVLAANRRYWR
                     PGWPKFDQLIFRFIPMKDQVQALLDGKTDVLTDLPGTMTLRVMENSSTQVVKKESMYT
                     VTGHFNTSKGILADVRVRRAIDHAINVQELIRYDLLANGVPMASLSFPGQVGYDATLT
                     PYKFDVPLAKRLLKEAGVQTPVRLTMVTVPFGERTAKIIAKQLEVLGLKVELSVYPDA
                     EIFQAFQNHTWDIGVAVLPSPIAHIAFPLTLMFFSQSPYSLHKSPEFDRLFLDAVTAL
                     DPVEQEKKFCAIVRYVHDQALGIFTYQRIKTYGVSRRVHFVPSVTGMAHFVDSTLVES
                     PSVGPQ"
     gene            3661..>5853
                     /locus_tag="PK362_01205"
     CDS             3661..>5853
                     /locus_tag="PK362_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC73492.1"
                     /translation="MARWNIKRLLPILGRWVALFRGRSLSRRILLPVLPAVLFLIGCM
                     GFFNYHLVRRQIVDSVRQTMNSDALNAARTLESFFQQRLSDLDSLSETPLIADYNKNR
                     GFGLVQEAEVYRRELEKYFANFSERSKVYYDISYVSPEGRRVCSLRQGISADTYQASF
                     PIGFLDFLHRGKRYDPPLQRIVEYGPLVKRYAKPIFDDNGTFLGAIVTDCDMKAVEDI
                     LRVIRVGKLGSAFLEDADGKAVLDVPAAHFTHPLRGEAPIHETETNWRWKVAVTAPAS
                     EFLDQPLKQIFYLTILFSVGICLLLMAMIVFRVSDLMNPIQSMVEGTRRFASGDLTFR
                     FPPLNSEELSTLAASFNKMAETLEIRNKELESRLRQLTALRDMEESVIQRQDEETVLR
                     TCLEAVARGFGFDRTGMYWVDHAHKEIVGRYLFGSDAAGFSEAAFRKRRVPLGGADIL
                     NEVVRSRSAIVVKNPVRDVRTNPSFVAEAKTREFVLAPICGKDRVLGILNADNFFTGR
                     PLTDADREGLMLYANAVGLALENAMLFQNLAESESRLRTVLENSPEAIIGLSREHWIN
                     TWNRGAEKIFGLSSAEVLGKPMTVLFPKGGGAEFKNLLNQVMERGAVKDFLMPGQTKD
                     GRLLDLSVSWGGAHADFWMNKEWTLVIRDITEARRLQQQLIRSEKLSAVGQLISGIAH
                     ELNNPLQAVVGYSDILTDDMRQKIDEASGKPVAVQPQEIVDDLRIITEN"
BASE COUNT         1165 a         1690 c         1703 g         1295 t
ORIGIN      
        1 catagacgtg gaaattcacg tgttccccga aggggaggcc atttcggcgt ttaagagcca
       61 accctggaac ttgggtttga cgacgctccc ttcccccatc gcccacgtgg cctttccgat
      121 gggattgttc ttttattcca attccttttt cagcatgcac cacgatccgg tttttgaccg
      181 aatgtatgaa aaagcgacgt cgatcatgga tccggcggaa agcgaaaagg ccttccagga
      241 attggaacgg aaggtgtacg acgacgcgct gggggtgttc ctgtatcggc ggattaagac
      301 ctacgccgtc agtaagcggg tgcgtttctc gccttacctc accggaatgc cgcattttgt
      361 ggacgccgtg tcgctcgtga aagaagaggc caataaaaaa tgattccggc gatatcgccg
      421 acggccatac cgatcctgtt ggtggtggcc ggaggcaccc acttcgccca aatcggggcg
      481 atgcattggt acgcgtacga gtgggcgcgc cgcaatcccc tgccggaagg tcccggggta
      541 ttgttccggg cccttcgaag cgggctcata tttatggtgg tcggcaccgg tctcctcgtg
      601 attttcaacg cgggacgaat tgccgcgggg ggcggattgg cggtgtcgct gtgcgcgttg
      661 ctctcgcttt ttgcgggata ccgcctgggg ctgcaatggt ttggcatggg caaagaaatg
      721 gaccacgccg gcaatcgtgg ggcgcgcgct gtcctctacg ccacgctcgt tcttcaattt
      781 ttgatttacg ccggggtgtt tctcgccctg gttcgttaaa taaggaggcc gattgatcca
      841 gacatccttg tgggcgaagg ttcgacgatg gagcggcgcg ttgtgggtgg tcgcgctggt
      901 ggtttccgca cgggcggcgc aaccgccgct gaatatgtgt gccacgacct tgttggacgg
      961 cggccccccg ccggggtttt actatttaaa ctacgccatt ttcaccgagg ggaagaaatt
     1021 tcgggacggc gacggcaacg aggccgcgaa catggggaaa ctgaacacct tttcccaact
     1081 gcatcagttc tattacatcg cggacttcaa tgtcttgggg gcgcacccgg ccctggatgt
     1141 cctggtgccc gtggccgctt taacggcgcg cggggggacc gcggggccct tctccctgaa
     1201 ttccaacacc gcgggattgg gcgatctggt cgtgggtccc gccctccatt gggacggccg
     1261 aacgttgttt ggaaaacccg tgttccatcg ggtggagctc gacctcacaa ttccgacggg
     1321 caaatacagc aaagaccaat tgttcaatcc agggtccaat cttcacacga tcgaggccta
     1381 ttattccttg gtgatgattc ttccaccgaa gtgggagacc agttggcggt ttttttacgc
     1441 tcaccactcc gaaaacaagg accatttgtt tggccggctg aagcccggcg cggtggtcca
     1501 cgccaattac gccctctccc gccaagtggg ggagaaatgg cgggtgggcg cggccggtta
     1561 ctatctacag caattgcggg aggacgaact gaacggcgcg aaacaggtca aatcgaagga
     1621 gcgggtcgtg tccatcggtc cggccgtgca ttacggagtc ccgggatggt ccctggtggt
     1681 gagtcatccc gttgaattcg ccgttcggaa tcgttttcaa ggttcccgaa cgaccctgca
     1741 actgattcac cggttctgac gtcgggaaaa ccccaccggg gcgccgccgg tgtttggcgg
     1801 tgctccggtg ttgtttttgg gtaacccgga ggaaggaaat gcgatctctg gtcttatgaa
     1861 aaaagcagcc cgttggttca tgccggaagg gtcccgtcgg tggcttcccg gcttagccgt
     1921 cttgctggtc ttggtggccc tcggtcgagc gagggaaagt ccctccacgg tcgtggtgtg
     1981 caacgacacc caggacccgc gcaccatgga tcccttcctg gagctcacgg aaaaaaatca
     2041 cgtccttctc cagcaaatga tggaaggtct ggtccgcttc gacccggagg gcaaggtcat
     2101 tccctgcttg gccgaatcct gggaacaggt ggatctcctt cggttgaggt tccatttgcg
     2161 caaggacgtg gaattccaca acggcgaacc ctttacggcc gaggccgtcc gtttcagcgc
     2221 ccaaaccttt tccgatccca agggggttta tcccggcaag gggttcgtct cctccatcga
     2281 ccgggtggag atcgtggatc cgttgaccgt cgatgtcgtc acgcatttcc ccgacggtat
     2341 attgttgcga agattcgccg gtttgatttt catggttcca cccgattact acaagaaagt
     2401 cggcagccaa ggcttttccg atcgtccggt tgggacgggg ccgtttcggt tcgttcggca
     2461 cgttccgggg aaggagatcg ttttggcggc aaacaggcgc tattggcgcc cgggctggcc
     2521 caagttcgat caattaattt ttcgctttat ccccatgaaa gaccaggtac aggcgctcct
     2581 ggacgggaaa accgacgtgc tgacggatct ccccgggacc atgacccttc gcgtcatgga
     2641 aaattcttca acccaggtgg tcaaaaagga gtcgatgtac acggtcacgg ggcatttcaa
     2701 cacgtcgaaa ggaattcttg cggatgttcg cgtccgacga gcgatcgatc acgccatcaa
     2761 tgttcaggaa ttgatccgtt acgatctttt ggccaacggc gtccccatgg ccagcctttc
     2821 gttccccggc caggtgggtt acgacgctac cttgaccccc tacaaattcg acgtccctct
     2881 ggcgaaacgc ctgctcaaag aagcgggggt tcaaacgccg gttcggttga ccatggtcac
     2941 cgtcccattt ggggagcgta cggccaaaat cattgccaag caattggagg ttctggggct
     3001 caaggtcgag ctgagcgtct accccgacgc ggagatattt caggcctttc aaaatcacac
     3061 ctgggacatc ggcgtggccg tgttgcccag ccccatcgcc cacatcgcgt ttccgttgac
     3121 cctcatgttc ttttcccaat cgccctacag ccttcacaaa agtccggagt tcgatcgttt
     3181 gtttttggac gcggtgacgg ccttggaccc ggtcgaacag gaaaagaaat tttgcgccat
     3241 cgtgcgttac gttcacgacc aagccttggg gatttttacc taccaacgga tcaaaaccta
     3301 cggggtcagc cgacgggtgc actttgtccc ttccgtcacc ggcatggccc atttcgtgga
     3361 cagcacgctc gtcgaatcgc cctccgtcgg accccaataa caagccatcc attatccaag
     3421 aaggaggaaa ggacaacctt tcttgctccg tgtttaaatg ggccaatcaa aaataggatt
     3481 ttgatgtaaa taaatgagat gtaacatttt tgcttttata tgatcgtggt tattgtgctt
     3541 cccgatttgc ggcactatat ttgcaaggtg cctctttcgg cgcttttcgc gttgtttttt
     3601 gagagtattt cttgtttcta aaaggcggtc aaaggtcccc gtttgggaaa taaaaacctc
     3661 atggccagat ggaatatcaa gcgattgctc cccattttgg ggcgctgggt cgcccttttt
     3721 cgggggcgga gcctcagccg ccgcattttg ttgcccgttt tgccggcggt gctgttcctt
     3781 atcggctgca tggggttttt caactaccac ctcgtaagac ggcagatcgt cgacagcgtc
     3841 cggcagacca tgaacagcga cgccttgaac gccgcccgca ccttggaaag ctttttccag
     3901 cagcggctga gcgacctcga ttccctttcc gaaacgcccc tgatcgccga ctacaacaaa
     3961 aaccgcgggt tcggcctcgt gcaagaggcg gaggtttacc gtcgggagct ggagaaatac
     4021 ttcgccaact tttccgaacg ctccaaggtc tattacgaca tctcttacgt gagccccgaa
     4081 gggcggcgcg tgtgctccct ccgtcagggc atttcggccg acacctatca ggcgtcgttc
     4141 cccatcgggt tcctggattt cctccaccgg ggaaaacgct acgatccgcc ccttcagcgc
     4201 attgtggaat acggtcccct cgtcaagcgt tacgccaaac ccatcttcga cgacaacggc
     4261 acctttctgg gcgccatcgt gaccgactgc gacatgaaag ccgtggagga catcctgcgg
     4321 gtcatccggg tgggcaaatt gggcagcgcg ttcctggagg acgccgacgg caaagccgtc
     4381 ttggacgtgc cggcggcgca ctttacacac cccctgcgcg gcgaggcccc catccacgaa
     4441 acggaaacca attggcggtg gaaagtcgcg gtgaccgcgc cggcgagcga attcttggat
     4501 cagcccttga agcaaatctt ttatttgacg atccttttct ccgtcggcat ctgtctgttg
     4561 ctcatggcca tgatcgtttt ccgcgtgtcg gacctgatga accccattca aagcatggtc
     4621 gaaggcacgc gccggttcgc ctcgggcgat ttgaccttcc ggtttccgcc cctcaacagc
     4681 gaggagttgt ccactctggc ggcctccttc aataaaatgg ccgagactct cgaaatccga
     4741 aacaaggagt tggaaagccg ccttcggcaa ttgaccgcct tgcgggacat ggaagagtcc
     4801 gtcattcaac gtcaggacga ggagaccgtt ctgcggacct gcctcgaggc ggtggcccgg
     4861 gggttcggtt tcgaccgcac cggcatgtac tgggtggacc acgcccacaa ggaaatcgtg
     4921 ggtcgctacc tgttcggctc cgacgcggcg ggattttcgg aggcggcgtt ccgaaaacgc
     4981 cgcgtgcccc tgggcggcgc ggacattttg aacgaagtcg tccgaagccg gtcggccatc
     5041 gtcgtgaaaa acccggtgcg ggacgttcga acgaacccgt ccttcgtggc ggaggcgaaa
     5101 acccgggaat tcgtcctggc gccgatctgc ggcaaagacc gggtgctggg catcctgaac
     5161 gccgacaatt tcttcaccgg ccgtcccttg accgacgccg accgggaagg cttgatgctc
     5221 tacgccaacg ccgtggggct tgcgttggaa aacgccatgc tgttccaaaa cctagcggaa
     5281 tcggaatccc gcctgcgcac cgtgctcgaa aactcgcccg aagccatcat cgggttgtcc
     5341 cgggagcatt ggatcaacac ctggaaccgc ggcgcggaga aaatattcgg gttgtcatcg
     5401 gcggaagtgc tgggcaagcc catgacggtc ctgttcccca aaggcggggg cgccgagttc
     5461 aagaacctgc tcaaccaggt catggaacgg ggcgcggtga aggacttcct gatgccgggc
     5521 caaacgaagg acggccgcct gctcgacctc tccgtttcct ggggcggtgc ccacgccgat
     5581 ttctggatga acaaagaatg gaccctcgtg atccgcgaca tcacggaagc gcggcggctc
     5641 cagcagcaat tgatccgttc cgaaaaactt tccgccgtgg gccagctcat ttccggcatc
     5701 gcccacgagt tgaacaaccc cctgcaggcc gtcgtcgggt attccgacat tttgaccgat
     5761 gacatgcgcc aaaaaatcga cgaagcgtcg ggaaaaccgg tcgcggttca accccaggag
     5821 atcgtggacg atctgcggat catcacggaa aac
//
LOCUS       DAOQLS010000034         5768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6090_length_5768_cov_10.209522, whole genome shotgun sequence.
ACCESSION   DAOQLS010000034 DAOQLS010000000
VERSION     DAOQLS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6090_length_5768_cov_10.209522"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..1656
                     /locus_tag="PK362_01210"
     CDS             394..1656
                     /locus_tag="PK362_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73493.1"
                     /translation="MRIPLKRTALGTNPAGERGAVLFFIIIMLTLIAVIGMASRLTVQ
                     SRRRTQDNLQKNQVRDIARGGILDALNWFRRQTVQPVASDLPVANRYPTPDDAFAPTT
                     STTTPDGGTIDASIGLVKEYELSPERNFWARHEVRRQGGGPSDPHAAHDITALRFANV
                     PAGEGRAWSLVSRGLVFERRDANAAFDQSPNRIIQEAFAYSEIQRFRTAVPPNCAVLT
                     PDAAASLFFRDFTTVDGNSAAPGVLTFSNPAVFNANGAVILGVPNPVVQQGAVSLTVQ
                     DFFGVPQSDLPAMSDLVLDDSILIQNQSQGFNSAAFDNTFVYIKGSASVPRFSISGSG
                     IIFVDGSAEVTGSFAGLIFVLGDLRMYQVQIRGAVVVTGALRSSVMSNSHSTANNVIF
                     DQNAVQLAIAGNSQYREVRQSFLLGADR"
     gene            1653..4646
                     /locus_tag="PK362_01215"
     CDS             1653..4646
                     /locus_tag="PK362_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73494.1"
                     /translation="MKRPRSSSGFTVMEVVFGLMLLGVLALVLGRTSLFINKHENDTQ
                     EKELANEICLQLMQEVRAYSEVNTVSDIDSLDDNTRFNSTLSISGSSPRNFAIANRYC
                     RQVNVFTVEGEPQARRVRVRVYRRGTPSFPVTGTALLAETTGFVRKTSKTALSIQNLR
                     MYRVVGFPSYPVYNSPTMKMDTRNISLVHAGRLPEGYSPRSVHHRTEPAYDWNYTDIV
                     TTSVYLTNNSDVNGSVDAYNHGTRQPTCSLGAPDCLRALLDRMILGDADYRNVLIVNA
                     GGVFYLPPIRNYSDAARDSGTIPYARAVTHPERIGVASGAEIKLRVYTYTMRPDLPAA
                     DTPLPEVTIVLSTNVPDANIAITKISGDSANAYTAGPAAVPADARVDVFASSTVIHLF
                     NSPQRHPYHHASHTGLRPNRRLYGLEYIPCPVKDGDWYDLTTDSDDEAKNTARWIIRL
                     APGSVPDGLFTATTRIGDDLTTGTFGNKPADKSVTYVWSGVAVPMTEQYQFIGDPRHM
                     PYMDVKQNHGYNWFFVRVNNHGYEGFDKTHDGWVYQHQLPNVNTTEERVNFDFPRYMQ
                     VIRTALLNSNSLYMVGDSSLGRNESYTPMAFASFGGGVTFEWTRLGVIPLKAPVNAWD
                     PNNSGVGYSFELGADRTNVNSYQRIIARTDNTWMSLPWLGELFPDESSATWTTLRNLY
                     AVPGGFYRAPYSVFASTIGLPAGTNFSMQIGGAALGTLLNGNTGGAGNNYVYMPYLGG
                     AFINYNTTWKAIENLFQIYMGRPSDNTTANIDLNAAGNPTRPPAYNDAPYAGERTRVT
                     VVRRFMDQRAGKETAALIRMEDPGNAARVGYVVVNTNENNDWNYELGAVQDFAMAAWG
                     FLEMGSPLEPLGVQPIPRVSFVAPLQDAVIQNPTTVPVQWNREWLRADGGAYTSDYPN
                     NYTHAGAVIFNLMYSDNNGSSWKDVATNANRSPAQRAQANNVTSPYNWDVSALPAGRY
                     VLRIEAFRAGEQHNAYHDRRIEIKR"
     gene            4618..5655
                     /locus_tag="PK362_01220"
     CDS             4618..5655
                     /locus_tag="PK362_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73495.1"
                     /translation="MTVELKSSVKSPPAVGYSLTEVVVAAALIGLLFLGFAPVFNMAR
                     RAMVGNEAKTALKSISESVMTRIDGNLREARRVYVRRITSWPANTAATVVDDFSAYVD
                     LTGCPPPLSGSQMHTRASTETVGNVLFFVKQLPPLQVVTGSTTTAVGVLVFYYYYVSL
                     APGGKSFGNGRERHLYEWKSVPYLEWGQLTRIGDAGLRSAVIEALRLQGGWRAYDVSQ
                     PLLDSAFYNLTGGGTLTLNPGHLIQRGEIAPLTTGINGRSLSGFSISVSPNTAVLPTG
                     TFKFTVPTTTFGTNWTGDSPSGFEVTNNGQNKSRGATLLRVRLVLLAQGPFDPFITRE
                     GVIASTVQDVW"
BASE COUNT         1119 a         1949 c         1597 g         1103 t
ORIGIN      
        1 ttccctcaaa gattatttct acctgttggg cgacactttc gtcacccgct accggtcgga
       61 gccctatgtg tccctggcca cgggccacct ctgcgtcacc ttgacggctc tcttccggga
      121 cgcccgcaac acgccccacg tcgtctgcct cgacgtgccc tgggtctgac gcggacgccg
      181 tccttgggcc tccgggagcg cgaaggttcc gtccccgggg aacaccgctc cttcgcctcc
      241 gccgccgtgc cccggcggcc ttgaaattcg cccattcccc gaccaccgcc ttggccgaag
      301 tgttgcattc ggtttcaaaa gacgccgccg ggagcccctt cttctgcatt tgctttcccc
      361 ccgacgcggg cctatactaa accgagaccg ccaatgagaa ttcctctgaa aagaaccgcc
      421 ttggggacga accccgccgg ggagcgcggc gcagtccttt ttttcatcat catcatgctc
      481 acattgatcg cggtcatcgg gatggcgagc cgcctgaccg ttcaatcccg ccgccggacc
      541 caggacaacc ttcaaaaaaa ccaggtgcgg gacatcgccc gcggggggat tctggacgcg
      601 ctcaattggt tccgccgaca gaccgtccaa ccggtggcga gcgaccttcc cgtggccaat
      661 cgatatccga cgcccgacga cgcttttgcg ccgaccacga gcacgaccac gcccgacggt
      721 ggaaccatcg acgcgtccat cgggctggtc aaagaatacg aactttcccc cgaacggaat
      781 ttctgggccc gccacgaggt gcgccgtcag gggggcggcc ccagcgaccc ccacgccgcc
      841 cacgacatca ccgcgcttcg cttcgccaac gtgcccgccg gcgaaggccg ggcctggtcc
      901 ctcgtttccc ggggcttggt gtttgaacgt cgggacgcca acgcggcttt tgaccaatcc
      961 cccaaccgaa tcatccaaga agccttcgcc tattccgaaa tccaacgctt tcgcaccgcg
     1021 gttcccccca actgcgcggt cctgacgccc gacgccgccg cgagcctgtt ttttcgggat
     1081 ttcacaacgg tggacggcaa ttccgccgcc cccggcgtac tgaccttctc gaacccggcg
     1141 gtcttcaacg cgaacggagc ggtgattttg ggggtcccga accccgtcgt tcaacagggg
     1201 gcggtttccc tgaccgtcca ggactttttt ggcgttcccc aatcggacct tcccgccatg
     1261 agcgacctgg tgctggacga ttccatcctc attcaaaacc agagccaggg gttcaactcg
     1321 gcggctttcg acaacacctt cgtttacatc aaagggagcg ccagcgttcc ccggttcagc
     1381 atttccggga gcggcatcat ttttgtcgac ggttcggcgg aggtgacggg cagcttcgcg
     1441 ggtttgattt tcgttctggg ggatctgcgg atgtaccaag tacaaatccg gggggccgtc
     1501 gttgtgaccg gcgctctccg atcctcggtg atgtccaatt cccactcgac ggccaacaac
     1561 gtcatcttcg atcaaaacgc ggttcaattg gccatcgccg ggaacagcca ataccgcgaa
     1621 gtgcgtcaaa gcttccttct gggggccgac cgatgaaaag accgcgatcg tcttcgggtt
     1681 tcaccgtcat ggaagtggtg tttgggttga tgctgctggg ggtgctcgcc ctcgtgttgg
     1741 ggcgcacgag tcttttcatc aacaagcacg aaaacgacac ccaggaaaag gaattggcca
     1801 acgaaatctg tcttcaactc atgcaggaag tgcgggccta ttccgaggtg aacaccgtca
     1861 gcgacatcga cagtctggac gacaacaccc ggttcaactc gactctttcg atttccggca
     1921 gttccccgcg gaatttcgcc atcgccaacc gctattgccg ccaagtgaac gtgttcaccg
     1981 tggaggggga accccaggcc cgccgcgttc gcgtccgagt gtatcggcgc gggaccccgt
     2041 cctttcccgt cacggggacg gcgctcctgg ccgaaacgac gggattcgtt cgaaaaacgt
     2101 cgaagacggc cctttcgatt caaaatcttc gaatgtaccg tgtggtgggt tttccgtcct
     2161 accccgtcta caactcaccg accatgaaaa tggacacccg gaacatcagc ctggtccacg
     2221 ccggccgact ccccgagggc tattcccccc ggtcggtcca ccaccgaacc gaacccgcct
     2281 acgactggaa ctacaccgac atcgtgacca cctccgtgta cttgacgaac aacagcgacg
     2341 tcaacggcag cgtcgacgcc tacaatcatg gaacgcgcca accgacctgt tcgttggggg
     2401 ccccggattg ccttcgggct ctgctggacc gaatgattct gggggacgcg gattatcgaa
     2461 acgtcctgat cgtcaacgcg gggggcgttt tttatctccc ccccatccgc aactattcag
     2521 acgccgcccg ggattccggg accattccct acgcccgggc ggtcacccac ccggaacgga
     2581 tcggcgtggc ctccggggcg gaaatcaaac ttcgggttta cacctacacc atgcggcccg
     2641 atttgccggc ggccgacacc ccgctccccg aagtgacgat cgttttgtcc acgaacgttc
     2701 ccgatgccaa catcgccatc accaagattt ccggcgactc ggccaacgcc tacaccgccg
     2761 gcccggccgc cgtgcccgcc gatgcccggg tggatgtgtt cgcttcgtca acggtcatcc
     2821 acctattcaa ttcgccccag cgacaccctt accaccacgc gtcccacacg ggcttaaggc
     2881 ccaatcgtcg cctttacggc ttggaataca tcccctgtcc cgtgaaagac ggcgattggt
     2941 acgatttaac caccgacagc gatgacgaag ccaaaaacac cgcccgttgg atcattcgcc
     3001 tggcccccgg ctcggtgccc gacggactct tcacggccac cacccgcatc ggcgacgatt
     3061 tgacgaccgg aaccttcggc aacaaaccgg ccgacaaatc ggtcacttac gtttggtccg
     3121 gggtggccgt gcccatgacc gagcaatatc aatttatcgg cgacccgcgc cacatgccct
     3181 acatggacgt taagcaaaac cacggctaca actggttttt tgttcgggtc aacaaccacg
     3241 gttacgaggg gttcgataaa acccacgatg gctgggtcta tcagcaccag cttcccaacg
     3301 tcaacacgac cgaagagcgg gtcaattttg attttccccg ctatatgcag gtgattcgca
     3361 ccgcgctgtt gaattccaat tcgctctaca tggtcggcga ttcctccctc ggccggaacg
     3421 agagctacac gcccatggcg ttcgcttcgt tcgggggcgg cgtgaccttc gaatggaccc
     3481 gcctgggggt gattcccttg aaagccccgg tcaatgcctg ggatcccaac aacagcgggg
     3541 tcggctactc ctttgaattg ggcgcggacc gcaccaacgt caattcctac caacgaatca
     3601 ttgcgcgcac cgacaacacc tggatgtcgc tcccctggct gggcgagctt ttccccgatg
     3661 aatcctccgc cacgtggacc acccttcgca atctttacgc ggtccccggg ggattctatc
     3721 gggcgcccta ttccgttttt gccagcacca tcggcctgcc ggccggcacc aatttttcca
     3781 tgcaaatcgg cggggccgcc ctgggcacgc tcttgaacgg gaacaccggc ggggccggaa
     3841 acaactacgt ctacatgccc tacctgggcg gcgcttttat caactacaac acaacgtgga
     3901 aagccattga gaatctattt cagatttaca tgggccgccc ttcggacaac accaccgcca
     3961 acatcgacct gaacgcggcg ggcaacccca cccggccacc ggcctacaac gacgccccct
     4021 acgccgggga gcgcacgcgg gtcaccgtcg tgaggcgttt tatggatcaa cgggcgggca
     4081 aggaaaccgc cgcgctgatt cgaatggagg acccggggaa cgccgcgcgg gtggggtatg
     4141 tcgtggtaaa caccaacgaa aacaacgact ggaattacga attgggcgcc gttcaggact
     4201 ttgccatggc ggcctggggg ttcctggaaa tgggatcccc gctggagccc ttgggcgtac
     4261 aacccattcc ccgggtgtct ttcgtggccc cgctccagga cgccgtgatt caaaacccga
     4321 ccaccgtgcc cgtccaatgg aacagggaat ggctccgggc cgatggaggc gcctacacct
     4381 ccgactatcc gaacaactac acccacgccg gggcggtgat tttcaatctg atgtattcgg
     4441 acaacaacgg ttcgagctgg aaagacgtgg ccacaaacgc gaaccgctcc cccgcccaac
     4501 gggcccaggc caacaacgtc acctccccct acaattggga cgtcagcgcc ctgccggcgg
     4561 gacgatacgt tcttcgcatc gaagccttcc gcgcgggaga gcaacacaac gcgtaccatg
     4621 accgtcgaat tgaaatcaag cgttaagtcc ccgccggcgg tcggctactc cctcacggag
     4681 gtggtggtgg ccgcggcgtt gatcggcctg ctgtttctgg ggttcgcgcc cgttttcaac
     4741 atggcgcggc gggcgatggt cggaaacgaa gcgaaaacgg ccttgaaaag catttccgaa
     4801 tcggtgatga cccgcatcga cggcaacctt cgggaggccc gccgagtgta cgttcgccgc
     4861 atcaccagct ggcccgccaa cacggccgcg acggtcgtgg acgacttttc ggcctacgtg
     4921 gatttaacgg gctgcccgcc gcccctgagc ggatcccaaa tgcacacgcg ggcctcgacg
     4981 gaaacggtcg gaaacgttct ctttttcgtc aagcaattgc ccccgctcca agtcgtaacc
     5041 ggaagcacga ccacggccgt cggcgttttg gttttttact attactacgt gtccctcgcg
     5101 cccgggggaa aatccttcgg aaacgggcgg gagcgccatt tgtatgaatg gaaaagcgtg
     5161 ccttatttgg aatgggggca gctgacccgc atcggggacg ccggtttacg gtcggccgtg
     5221 atcgaagctc tgcggttgca agggggatgg cgggcctacg acgtgtcgca gccgcttctg
     5281 gacagcgcct tctacaacct caccggcggc ggaacgctca cgttgaaccc gggccatctc
     5341 atccaacggg gggaaatcgc tccgctcacc acgggaatca acgggcggtc cctgtcgggg
     5401 ttttccattt ccgtaagccc caacaccgcc gttcttccca cgggaacttt taaattcacc
     5461 gtcccgacga cgacctttgg aaccaactgg acgggtgatt ccccctcggg atttgaagta
     5521 acgaacaacg gccaaaacaa gtcccggggc gccactttgt tgcgcgtccg gctcgttttg
     5581 ctggcccagg gtcccttcga tcccttcatc acccgggaag gcgtcatcgc ctccacggtg
     5641 caggacgtct ggtaaacccg cccgcggccg gaccgaaacc cggcctcgac cctaggaacc
     5701 gatcgcctcg gccagcgcga aggttccgtc cccggggaac accgctcctt cgcctccgcc
     5761 gccgtgcc
//
LOCUS       DAOQLS010000035         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6213_length_5704_cov_4.063728, whole genome shotgun sequence.
ACCESSION   DAOQLS010000035 DAOQLS010000000
VERSION     DAOQLS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6213_length_5704_cov_4.063728"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..606
                     /locus_tag="PK362_01225"
     CDS             175..606
                     /locus_tag="PK362_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73496.1"
                     /translation="MAQFFKKRRRGAPGPSKNELKVRRRRADANEAAQQRPLAEEIPG
                     VQRVTLQLDFLTPQRQVLDQQTHVFNATDTPEFTAECLGRCGGTGIFDLSDKVQQAVN
                     ARQRTLQSTDLCAERIFPGSSETCGFQLQSTIDIVFAPANG"
     gene            692..1321
                     /locus_tag="PK362_01230"
     CDS             692..1321
                     /locus_tag="PK362_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC73497.1"
                     /translation="MKKWLWILPGLLSVSSARAYDATLKAFQGKAFVRSAGETAYRPL
                     RPGQKFRRDDTLRTGDGGLVQVAFKSGATVLVKENSRFSLRSDKNGDALTFVRGEFLI
                     GLRNRLKGRDKFTVRTPACVAAVRGTVFWGLSDDKKKSTYACFTGAIEVTARGRSVTL
                     NPGQKLAVAFGAAPSKTVPANIPKEYIKTFAVDDSLQGVDEILEDGGAK"
     gene            1757..1843
                     /locus_tag="PK362_01235"
     tRNA            1757..1843
                     /locus_tag="PK362_01235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1791..1793,aa:Ser,seq:tga)
     gene            1961..2203
                     /locus_tag="PK362_01240"
     CDS             1961..2203
                     /locus_tag="PK362_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC73498.1"
                     /translation="MGKSCESCLMPLRKDPGPRESDRYCSYCFKNGALCYSGTDLKEF
                     QRRAYEGMIARGTGRIAARLFTFLIRFAPRWRAPKN"
     gene            2216..2671
                     /locus_tag="PK362_01245"
     CDS             2216..2671
                     /locus_tag="PK362_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73499.1"
                     /translation="METPKTTEVAPVPLGQKIKRALIKYSLLALLAAIGGSALWVWGS
                     LTYVYSSGERAGYVQKISKKGWVIKTWEGELAMVNLPGAMPEKFAFTVREESVARRIQ
                     DTIGQRVALSYQEHRGLPGNLFGDTRYFVINVGAVDPAPTPADAPRSAL"
     gene            complement(2660..4579)
                     /locus_tag="PK362_01250"
     CDS             complement(2660..4579)
                     /locus_tag="PK362_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73500.1"
                     /translation="MVLKSGRRAMALGAALWMGLSPAVKAAPVFVRTAALTLAGNSNL
                     VTAAVDAAGTNAYFVNGTGTVTKIRLSDFTELSSLTVAGVTSVGAAAIDTGLNQLYFT
                     AKVSGVVKLVRVNLGSFTHAPGTDQLTLRSDAINVPTAFAVDAAGHLGYVAAGKSSAS
                     NPAEMIRVNLSTFAVSFRKNATFPDDLNTVAGALDGSYFYGPSTTIYLSGRFAIGRVI
                     LSSLALDAPTAQPSGSPSPRIGLIDTTAGEAFFTTTAGVVWRVKTGSFPSAASVALAG
                     SPNPASGIIDRQNRFLFVGTSESPGKIKRVDLATFAASGELTMNAGENNLSCAVYDSV
                     AGYGYFGTNTSPARVVKVQLSNFAGSPPVNLTLPTLSGSAIQTQTLTGADGSWGGSPS
                     GFARQWRRCNSAGAACVDIGGATGTTYLLGVADVGSTIRFRVTATNGSGSTSADSAAS
                     AVVQASVVAVSSLSLSPSTVQGGTATHGTVTLGSAAPAGGSSVALASADTAVASVPSS
                     VLVAAGQTSAGFTIDTFAVAANTPVAVSATAGGQTRNATLTVTAESEEKPPHVFPNPY
                     RPAAAGSLTLQDLPVQTAVKVVAADGRFVRSLTTDVNGAASWDGLTESGEPAPSGIYL
                     VLVEKRAPVKIALQR"
     gene            complement(4570..5478)
                     /locus_tag="PK362_01255"
     CDS             complement(4570..5478)
                     /locus_tag="PK362_01255"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC73501.1"
                     /translation="MKNLLAAGGVLLALSISVPAATTEGLSSPFLSMGAGARAAALGQ
                     AYTAVANDATAVYWNPAGLPFLHRRTVTLMHGALVGSAMTYDYAAFGQKWGRRGGVGI
                     GIQHLSAGGLVLADATGARQGQFKPRDFAGSLALGGQWSGGAVGAAVKLVRRQVVDTA
                     QTLTYDAGLLSPFYFGNRLRLGYAATNLGGSLKFDQVEEKIPATHRGGAVIRFSPAAF
                     ASVDMEKKTGAPAALRAGVEYALDLKTGALALRAGYDSGLTEDHGAAGFTAGLGMGFD
                     NVKVDYALIPNREGDVSHVVSLTTWF"
BASE COUNT         1061 a         1888 c         1813 g          942 t
ORIGIN      
        1 ccccaagggc ggcggcgcgc ccgccggccg ttccggtcgg ccgccctttc aatcccacaa
       61 gggatcgtcc cattcgcccc acaaagggtc gtcccacccg aacgcgcgac accccggaaa
      121 ccggcccccc cgcgggccct tccgtcgaca caaataaccc gaggagaaac caccgtggcc
      181 caatttttca agaaacgacg ccggggcgcc cccggaccca gcaaaaacga actcaaagtc
      241 cgccgccgac gggccgacgc caacgaagcc gcccaacagc gcccgctggc cgaggaaatc
      301 cccggcgttc agcgcgtgac gctgcagctc gatttcctga cgccccagcg acaggtgctg
      361 gaccagcaaa cccacgtctt caacgcgacc gacacgccgg aattcacggc cgaatgcctg
      421 ggccgctgcg gcggcacggg gattttcgat ctttccgaca aggtccagca ggccgtgaac
      481 gcccgccagc gcaccctcca aagcaccgac ctctgcgcgg agcggatttt tcccggctcc
      541 agcgaaacct gcggcttcca gcttcaatcc acgatcgaca tcgttttcgc ccccgccaac
      601 ggctgatcgc ggcgccgccg cccaccgccc aacatatata atggtttctc ggcgcgcccc
      661 ttcgggcgcg gcccagaaag gcggctcctt catgaaaaaa tggctttgga ttcttcccgg
      721 gcttctctcg gtgtcttcgg cccgggccta cgacgccacc ctgaaggcct tccaggggaa
      781 ggccttcgtc cggtccgcgg gcgaaaccgc ctaccggcct ctgcgccccg gtcaaaaatt
      841 ccgacgagac gacaccctcc gaaccgggga cgggggattg gtccaggtgg ccttcaaatc
      901 cggcgccacg gtcctggtga aggaaaattc gcgcttttcc ctccgcagcg acaagaacgg
      961 cgacgccctg acctttgttc gcggcgaatt cctgatcgga ctgcgaaacc gcctaaaagg
     1021 gcgcgacaag ttcacggtcc gcacgcccgc ctgcgtggcc gccgttcggg ggacggtgtt
     1081 ttggggattg agcgacgaca agaaaaaatc cacctacgcc tgcttcaccg gggccatcga
     1141 ggtcaccgcc cggggtcggt cggtgacgct gaaccccggg cagaaattgg ccgtggcctt
     1201 cggcgccgcg cccagcaaga ccgtccccgc gaacatcccc aaggaataca tcaagacctt
     1261 cgccgtggac gacagcctgc agggggtgga cgagattttg gaggacggcg gggcgaagta
     1321 agttccactc gcccacaact taattgatat tcggcgccgt cccggcgcct ctcgccgggg
     1381 cgacacttgg ccggagtcca agtgcgcatg aagccgcgga gcggcgaggc ggcgctcgaa
     1441 accccgaatt ggaatctaat ttggctggca ctttcgcaaa gcttcaagtg ccagcggcgg
     1501 gcccagaggg cccgaaaccc cgctggaggg gtggccgggt cggggcgacg ggcgaaggcc
     1561 gtcgcatgat tggggggatt tcctcacgac aacggcgggg ccgttgtgga acgaggccac
     1621 cggaagagtt ccaacctttt ggaggggtgg ccgggtcggg gcgacgggcg aaggccgtcg
     1681 catgattggg gggatttcct cacgacaacg gcggggccgt tgtggaacga ggccaccgga
     1741 agagttccaa ccttttggag gggtggccga gtggtttaag gcggcggtct tgaaaaccgc
     1801 tgtgcccgaa aggacaccgg gggttcgaat ccctccccct ccgatttttt taaagtgtga
     1861 tttgggattc gaagaatccc gaccgcgcta gcggaagtgg cgcgggggag cggcctcccc
     1921 ctccgatttt ttgattccga ccaacgcccg gaggccgtcc atgggaaaat cctgcgaaag
     1981 ctgtttgatg cccctacgga aggaccccgg gccccgggag tccgaccgtt actgcagtta
     2041 ctgctttaaa aacggcgccc tgtgctattc ggggacggat ttaaaggaat ttcagcgtcg
     2101 ggcctacgag ggcatgattg cccgcgggac cggccggatc gccgcgcgcc tttttacgtt
     2161 tctgattcgc ttcgcccccc gttggcgggc gccaaaaaat taaaggacaa attccatgga
     2221 aaccccgaaa accaccgaag tcgcccccgt gccccttggg caaaaaatca aaagggcctt
     2281 gatcaaatat tcccttttgg cgttgcttgc ggccatcggc ggatcggccc tctgggtgtg
     2341 gggatcgttg acctacgtgt attcctcggg cgaacgggcg gggtacgtcc aaaaaatttc
     2401 caagaagggc tgggtgatca aaacctggga gggcgaactg gccatggtca atctgccggg
     2461 agcgatgccg gaaaaattcg cctttaccgt ccgcgaggag tccgtggccc ggcggatcca
     2521 ggacaccatc ggccaacgcg tggcgctttc ctaccaggaa caccgcggat tgcccggaaa
     2581 cctttttggg gacacccgct atttcgtcat taacgtcggc gcggtggacc cggcgccgac
     2641 tcccgccgac gctccgcgct cagcgctgta aggcgatctt gaccggggcc cttttttcca
     2701 ccaacaccag gtaaataccg ctcggcgcgg gctcgccgct ttccgtcaac ccgtcccagg
     2761 acgccgcccc gttgacgtcc gtcgtcaacg accgcacaaa ccgcccgtcc gccgccacca
     2821 ccttcaccgc cgtctggacc ggaaggtcct ggagagtcaa cgaacccgcc gccgcggggc
     2881 ggtagggatt ggggaacacg tggggcggct tctcctcgct ctcggccgtg accgtcaacg
     2941 ttgcgtttcg ggtttggcct ccggccgtgg cgctcacggc gacgggggtg ttggcggcca
     3001 cggcgaaggt gtcgatggtg aaccccgcgc tggtttgtcc cgccgccacc aggacgctgg
     3061 agggaacgct ggccacggcc gtgtcggcgc tggcgagggc cacggaactg ccccccgcgg
     3121 gcgcggcgga acccagcgtc accgtgccgt gggtggcggt gccgccttgg accgtggacg
     3181 gcgaaaggga caggcttgag accgccacga ccgacgcttg aaccacggcg ctggcggcgg
     3241 aatccgccga ggtggaaccg ctgccgttgg tggcggtgac gcgaaagcga atggtgctgc
     3301 ccacgtccgc cacgccgagg aggtaggtcg tccccgtcgc gccgccgatg tcgacgcagg
     3361 cggctccggc ggaattgcag cgccgccact ggcgggcgaa cccgctcggg ctgccgcccc
     3421 aggacccgtc ggcccccgtc aacgtttggg tttgaatggc ggaccccgac agggtcggca
     3481 acgtcaaatt caccggcgga gaccccgcaa aattggaaag ctgcaccttc accacccggg
     3541 ccgggctcgt gttggtgccg aaatagccgt accccgccac ggaatcgtac accgcgcagg
     3601 acaggttgtt ttcccccgcg ttcatcgtca attcgcccga cgccgcgaag gtcgccaaat
     3661 ccacccgctt gattttcccc ggggattcgc tggtgcccac gaacaaaaat cgattttgcc
     3721 ggtcgatgat cccgctggcg gggttgggcg atcccgccag agccacgctg gcggccgagg
     3781 ggaaggaacc ggtcttcacc cgccagacca cgcccgcggt cgtcgtaaaa aaggcctccc
     3841 cggcggtcgt gtcgatgagg ccgatccggg gcgagggcga ccccgacggc tgggccgtgg
     3901 gggcgtcgag ggcgagactg gaaagaatga cccggccgat ggcgaaccgg ccggacaggt
     3961 aaatggtcgt ggaagggccg taaaagtagg aaccgtcgag ggcccccgcc acggtgttca
     4021 aatcgtcggg aaaggtggcg ttcttccgaa aggagacggc gaaggtcgac aaattgacgc
     4081 gaatcatttc ggccggattc gaagcggagc ttttgccggc ggccacgtag cccaggtggc
     4141 cggcggcgtc caccgcgaag gccgtgggca cgttgatggc gtccgaacgg agcgtgagct
     4201 ggtcggtccc cggcgcgtgg gtgaaggaac ccaaattgac gcgaaccagt ttgacgacgc
     4261 ccgacacctt ggcggtgaaa tacaactggt tgaggcccgt gtcgatggcc gccgccccca
     4321 cggaggtgac gccggccacg gtcagagagg aaagttccgt gaaatcgctc aagcggattt
     4381 ttgtgacggt ccctgtgccg ttcacaaaat aagcgttggt tccggccgcg tcgacggcgg
     4441 cggtgaccaa attggaattc cccgccaggg tcaaggcggc ggtccggacg aagacgggcg
     4501 cggccttgac ggccggggac agacccatcc acagggccgc gcccagggcc atcgcccgtc
     4561 gtccgctttt tagaaccacg ttgtcagcga caccacatgg gacacgtcgc cctcccggtt
     4621 cggaatcaac gcgtaatcca ccttcacgtt gtcaaaaccc attcccaggc ccgccgtgaa
     4681 acccgcggcg ccgtggtctt ccgtcaatcc gctgtcgtag cccgcccgca gggccaaggc
     4741 gccggttttc aagtccagcg cgtattcgac gcccgcccga agggcggcgg gggcgccggt
     4801 ttttttctcc atatcgaccg acgcgaaagc cgccggggaa aaccgaatca cggccccgcc
     4861 ccgatgcgtg gcgggaatct tttcttccac ttggtcgaat ttcaacgaac cgcccaaatt
     4921 cgtcgccgcg tacccgaggc gcagacggtt gccgaaataa aacggggaga gaagccccgc
     4981 atcgtaggtc agggtttggg ccgtgtccac cacctgccgg cggaccagct tgaccgcggc
     5041 gccgacggct ccgccggacc attgaccgcc cagcgccaag gaaccggcga aatcccgggg
     5101 cttgaactgc ccttggcggg ctccggtggc gtcggccaac acgagccccc cggccgacaa
     5161 atgctgaatt ccgatcccca cgcccccgcg ccggccccat ttttggccaa aggccgcgta
     5221 gtcgtaggtc atggcggacc ccaccagagc gccgtgcatc aacgtcacgg tgcgccggtg
     5281 caaaaagggc aggccggcgg gattccaata gacggcggtc gcgtcgttgg ccacggccgt
     5341 gtaggcctgg cccagggccg cggcccgggc gccggccccc atggagagaa acgggctcga
     5401 caggccttcc gttgtcgccg ccgggacgga aatcgacaaa gcgaggagaa cgccgcccgc
     5461 ggccaaaagg tttttcatct ctctaatata agttcaattg acccgcctga caagttcgat
     5521 tgccgaaata ttttcccgcc ggtggatttt taacggcact tctactatcc ttgaacatca
     5581 tgacccgccg cttcccgcgc ttttttttcc cgctcttcct tttggcgtgg gccgccgggc
     5641 tgcccctcgc cgcccgggcc gcgctcccgc ccaggtccgt ttacgaagaa aaattaaagt
     5701 ccgg
//
LOCUS       DAOQLS010000036         5648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6326_length_5648_cov_3.917576, whole genome shotgun sequence.
ACCESSION   DAOQLS010000036 DAOQLS010000000
VERSION     DAOQLS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6326_length_5648_cov_3.917576"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK362_01260"
     CDS             complement(<1..376)
                     /locus_tag="PK362_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73502.1"
                     /translation="MKMVSAGMVLLVLAGATALRAADRYALEVPGAVMAGDAFELKVI
                     PRSGAGRDGAPQRVKLEFPAGFIAAGAPRFLDLQGEGVVSVKTLPTVAPGLYALSVRS
                     AGDRRVLGMVDIAVERPAASFSL"
     gene            complement(660..5534)
                     /locus_tag="PK362_01265"
     CDS             complement(660..5534)
                     /locus_tag="PK362_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC73503.1"
                     /translation="MNWGEQEKALAAWARERGLAAVHSPAGRERSEEIRKIVNKELVG
                     LATPERVLPGARALLEFLRKAGVRLEVATSGSVETRTRVLRGLGLSEFFGTLHGGGKK
                     GDVIGRRLSEEGLTGTHAVLIGDGRGDIAAARATGALAVGVASDPERAAVLRAAGADV
                     IVHGDFSDLAALRPALGFGDRRGTPPWRAGPAEGDVRTLSPPPGRGLVFPPTLRFDNV
                     LGNYKAAVSEAPTDRRYMRSVALQKLPGLSAQTGFDVLLSNQGLQRTGSFKPWILSGV
                     LGRVARDPGAFGRTHRFLTTPTTGNQGQSLAFFADFLRRTYPRELGHLQAVVFVPRNA
                     PAGKIERMRAGGADIRFTTDYAEAEVRSREFAEREGAYFLAHGDERAVESYGALGLDL
                     LVALAHREAPDVGLDRLVRWFDLKARSASLEAAESEELSALETDLAGLADTAFLIPVG
                     AGGVVAGVDFALKSIDPRLTVIGVDSSRADPMSQSLARGEIAAQSPAPDILENGIAVN
                     APEPLAFHTAAGLLDAMVTVPFESVAAGVRAHWEEAGVVSEGAAALPYAALARSPALV
                     DNLRRRGVRRVVLLGTGQNIPEDLWASLTGASGFPAGPRGGGMHHAGEADLNSDQWRA
                     LRSRARREASAEERRRWAADLRARVDRQRDWLDPLELTGPLLALADQLEQKKPLAGGA
                     LASLGVQRRPSPVRWFVLKPVAGESWADRIEGAGERVPARGRSPEEVRLYVTGLAAVI
                     HEGLEHAVLPGVLGLNPRDRHTLAYLAEALANDWDEGPVPERTLEEMGRRRAVQLVSF
                     VERSRAEFNRVRRRFGDRPAVRERLEAIVRRLRDHAELCLSDLRAYPKPNAFFDAFRA
                     ADETYWTGNTPEFGSSVRKLDRIVRQASVEIQRPLPLWRSVARRLRGRNAPWAAGPGR
                     AAKDQNLPPRLTALQRSWDHYQFPEFSTALAALAAGARERPLPGGARDMLRLWAAADQ
                     VVEPLRRRGDWSETRVLQSEFVRWVRAEAVDRGAGSPYWEKTALRLLRDLSTDAVNAG
                     GLSAEAAGEFRWAFDLLDRADREGAPALSLSEQRRLLAILAGGETTPLERGVLYEKAR
                     NALTKFKGSFREKLEDVTLRAARFNVPKGRPSPIELLAVVTWSSDLAFEDLVAILRDY
                     LSGRLSRDRAESRLIQGGNAARSAVAMALDQWDEGLDPVSAASGRLLPREVSRWTLRA
                     AGAGATLLFFVSTWGALAGSSVDVRWAAPLFAPAMLLGALRPWLRAASARGPGARAPA
                     AFLARARGDAATSFLEVVDGDLRVNPAIRDRWSRWPVPIQNALLYGLGVVGHESLHRA
                     GIRSESVAYGLGQLLPAFLGAGAAALLLGAASPGAALASLAGAPAAVAAAGILLDRWA
                     ERRAEAVASALARAAAGQSLAVDFQALLASRPMRLGVAGEAGSGDRLDGVDDPGFGAA
                     LRRSLSRRGISGSPVEIQALLVRALGASGLVAPPSAAGDRPLTVVLVSRSTADRLEAV
                     IARSRPNGEGAAPVLALLPLDAEGEAAIARWNPADRRVERWTRRIHRSLESDPWDLRE
                     VESTVRSRLNPSEFSSCRILAGADVPLRAGGLETALFRDAMVWLMDGLFPVSVTTSDL
                     DRLTRLSRLVATAA"
BASE COUNT         1032 a         1983 c         1867 g          766 t
ORIGIN      
        1 cgagcgaaaa cgacgccgcc ggtcgctcga cggcgatatc gaccattccc aaaacgcgcc
       61 gatcgccggc gctccggacg gacaacgcgt agagaccggg cgccacggtc gggagcgttt
      121 ttacggacac gacgccctcg ccctgcaagt ccaggaagcg cggcgctccc gccgcgataa
      181 accccgccgg aaattccagt ttaacgcgct ggggagcgcc gtcccgcccc gcgccggacc
      241 ggggaatgac cttcaattca aaggcgtcgc ccgccatcac cgcgccgggg acttcgaggg
      301 cgtagcggtc cgcggcccga agggccgtcg ctcccgccaa caccaacaag accatccccg
      361 cggagaccat tttcatcgcc gaacccaccg tcctttcccc cccgcacgcc gagccgcccc
      421 gggcgatgga ctcccccggg aaaagcgcgc gttggattgc tcgggattta aaccatgggc
      481 agcaaaaccg ggccgtttgc aaaaagtgtc ctctcgatgt gccgggccga tataaggaag
      541 ggtgttcgcc cggggccacg tagcaagtct agggccgaat cttaaaattt tcaagcccag
      601 gatttatagg gattttacac gatggggggc gcccccgaaa tggccgattt gttgcggggc
      661 taggccgcgg tggcgacgag gcgggaaagg cgggtcaggc ggtccaaatc cgacgtggtg
      721 acggaaacgg gaaagaggcc gtccatcagc cataccatcg cgtcccgaaa gagagccgtt
      781 tcaagcccgc cggcgcgcag gggaacgtcg gccccggcca gaattcggca ggaggaaaat
      841 tcggagggat tgaggcggga tcggaccgtc gattccacct cccgcaggtc ccagggatcg
      901 ctttccaagc ttcgatgaat tcggcgcgtc caccgttcca cccgtcggtc cgccggattc
      961 catcgggcga tggcggcttc cccctcggcg tccagcggga gaagggccaa caccggggcc
     1021 gcgccctccc cgttcgggcg ggaacgggcg atcaccgcct ccaggcggtc cgccgtgctt
     1081 cgggaaacga ggacgacggt caaggggcga tccccggcgg cggacggagg ggcgacgagg
     1141 ccggaggccc cgagggcccg gaccagcaag gcttggattt ccacggggga accggaaatt
     1201 ccccggcggc tcaacgacct cctcagagcc gccccgaagc cgggatcgtc cacgccgtcc
     1261 agccgatcgc ccgatcccgc ttcgcccgcg accccgaggc gcatgggccg ggaggccaaa
     1321 agggcttgga aatcgacggc gagcgattgc cccgccgctg cccgggccag ggcggacgcg
     1381 acggcctcgg cccgccgctc cgcccaacga tccaatagaa tcccggcagc ggccaccgcg
     1441 gcgggagccc cggccaggga cgccagggcg gccccggggg aggcggcgcc caacagcagg
     1501 gccgccgctc ccgccccgag gaaagcgggc aataattggc cgaggccgta ggccaccgac
     1561 tcggaccgga tcccggcccg atgaaggctt tcgtgaccca ccaccccgag gccgtaaagc
     1621 agggcgtttt gaatcggcac cggccaccgg gaccaacgat cccggatcgc cgggttgacc
     1681 cgaagatccc cgtccaccac ttccaggaaa gacgtggcgg cgtctccccg ggcccgggcc
     1741 aggaaggcgg ccggagcgcg ggcccccggc cctcgggcgg acgccgcgcg aagccagggc
     1801 cgcaaggccc ccagcaacat cgccggggcg aagaggggcg ccgcccaacg aacatcaacg
     1861 gacgatcccg ccagcgcccc ccaagtggaa acaaaaaaga gcagggtcgc cccggcgccg
     1921 gcggcgcgaa gggtccaacg ggacacctcc cggggaagca gccgaccgct ggcggcggaa
     1981 accggatcca acccttcgtc ccattggtcc aacgccatgg ccaccgcgga ccgcgcggcg
     2041 ttcccgcctt gaatcaaccg gctttcggcg cggtcccggg acaatcgccc cgataaataa
     2101 tcccgaagaa tggccacgag gtcttcaaag gccaaatccg aggaccaagt gacgaccgcc
     2161 agcaattcga tgggggacgg gcgccccttg ggcacgttga aacgggcggc gcgaagagtc
     2221 acgtcctcca acttttcacg aaaggaccct ttgaatttcg tcagggcgtt tcgggctttc
     2281 tcgtacagga cgccccgttc cagcggcgtg gtctcccccc cggccaaaat cgccagcaag
     2341 cgtcgctgct cggacaggga cagggccggc gccccctccc ggtcggcgcg atcgaggagg
     2401 tcgaacgccc agcgaaattc cccggcggcc tcggccgata gtcctccggc gttcacggcg
     2461 tccgtcgaca aatcccgcag gagacgcagg gccgtttttt cccaataagg ggaacccgcc
     2521 ccccggtcaa cggcttcggc ccggacccac cggacgaatt ccgactgaag aacccgggtc
     2581 tcgctccaat ccccccgacg gcgaagcggc tccaccacct ggtccgcggc cgcccaaagg
     2641 cggagcatgt cccgggcgcc gccgggcagg gggcgttccc gggccccggc cgccaaggcg
     2701 gccagggcgg ttgaaaattc cggaaattgg tagtgatccc aggaccgttg cagagccgtc
     2761 aatcgcggcg gaaggttctg gtccttggcc gcccgaccgg ggcccgcggc ccaaggggcg
     2821 ttccgacccc gaaggcgtcg ggcaacgctc cgccacaggg ggaggggccg ttgaatttcg
     2881 acggaggctt ggcgtacgat tcggtccaac tttcgaacgg aagaaccgaa ttccggcgtg
     2941 tttcccgtcc agtaagtctc gtccgccgcg cggaaggcgt cgaaaaacgc gttgggcttg
     3001 gggtaggccc gaaggtccga aaggcagagt tccgcgtgat cgcgcaggcg gcgcacgatg
     3061 gcctccaacc gctcgcgtac cgcggggcgg tcgccgaacc gtcggcgaac ccggttgaat
     3121 tccgcgcggg atctctcgac aaaactgacg agttgaacgg cccgccggcg gcccatttcc
     3181 tccaacgttc gctcgggaac gggcccctcg tcccagtcgt tggccagcgc ctcggccagg
     3241 taggccaggg tgtggcggtc ccggggattg agtcccaaaa caccgggaag aaccgcgtgt
     3301 tccaatcctt cgtgaatgac cgccgccaaa cccgtgacat agagccggac ttcctcgggc
     3361 gaacgacccc gggccggcac ccgctcgccg gccccttcga tccggtcggc ccaggactcc
     3421 ccggccaccg gcttcaacac aaaccatcga accggcgacg gccggcgctg aacccccagg
     3481 gaggccaagg ctccgcccgc caggggcttt ttttgctcca gctggtccgc caacgccagg
     3541 agagggcccg tcaattccaa gggatcgagc cagtccctct ggcggtcgac ccgggcccgg
     3601 agatccgccg cccagcggcg acgttcctcc gccgaggctt cgcggcgggc gcgggagcgg
     3661 agggcgcgcc attgatcgga atttaaatcg gcttcgcccg cgtggtgcat gccgccgccc
     3721 cggggcccgg cggggaagcc cgacgcgccg gtgagagacg cccaaaggtc ttcgggaatg
     3781 ttttggcccg tgcccaacag gaccacgcgg cggacgcccc ggcgccgtaa attgtcgacg
     3841 agggccgggg accgcgccaa cgccgcgtag ggcagggcgg cggcgccttc ggaaacgaca
     3901 ccggcctctt cccaatgggc ccgaacgccc gcggccacgg attcaaaggg cacggtcacc
     3961 atggcgtcca atagacccgc ggcggtgtga aaggccagag gctcgggcgc gttcaccgcg
     4021 atcccgtttt ccaaaatgtc cggggccggg gactgggcgg cgatctcccc ccgcgcgagg
     4081 gattgggaca tggggtccgc ccgggaagaa tccacgccga tcaccgtcaa acggggatcg
     4141 atggatttca gggcgaaatc caccccggcc acgacgcccc ccgctcccac cgggatcaga
     4201 aaagccgtgt ccgccaatcc cgcgaggtcc gtttccagcg ctgataattc ctcggattcg
     4261 gcggcctcca aggaagccga ccgggccttg agatcgaacc atcggaccaa ccggtcgaga
     4321 ccgacgtcgg gggcctcccg gtgagcgagg gcgacgagca aatccaaccc cagggcgccg
     4381 taggattcca cggcccgttc atcgccgtgg gcgaggaaat aggcgccctc ccgctcggca
     4441 aattcccggg aacgaacttc ggcttcggcg taatccgtcg tgaagcggat gtccgcgccc
     4501 ccggcccgca tgcgctcgat ttttccggcg ggggcgttcc gcggaacgaa cacgaccgct
     4561 tgaaggtgcc ccagttcccg ggggtaggtt cgtcgaagaa aatcggcgaa aaaggcgagg
     4621 gattgcccct gatttcccgt ggtcggcgtc gtgaggaatc gatgggtccg gccgaaggcc
     4681 ccggggtccc gggccacccg gcccaacacg cccgacagga tccagggctt gaaggacccg
     4741 gttcgctgaa ggccctggtt gctgagcaga acatcaaatc cggtttgggc gctgagcccg
     4801 ggaagttttt ggagggcgac ggaacgcatg taacgccggt ccgtcggcgc ttccgaaacg
     4861 gcggccttgt aatttcccaa gacgttgtca aaacgcagcg tcggggggaa cacgagaccc
     4921 cgtccggggg gcggcgacag cgttcgaacg tccccctcgg cgggcccggc gcgccaaggc
     4981 ggcgtccccc gacgatcgcc gaacccgagc gccgggcgaa gggccgccaa atccgaaaaa
     5041 tccccgtgaa cgatgacgtc ggccccggcg gcccgaagga cggcggcccg ctccgggtcc
     5101 gacgcaacgc ccacggccag ggcgccggtg gcccgggccg cggcgatgtc gccccgtccg
     5161 tcgccgatca ggacggcgtg ggtcccggtc aatccttcct cggacaaccg acgcccgatg
     5221 acgtccccct tttttccccc gccgtgaagc gtcccgaaga attccgacaa cccgagccca
     5281 cgcaggaccc gggtccgcgt ttcgacggaa ccgctggtgg cgacttcgag acgaaccccc
     5341 gctttccgta gaaattccag caaagcgcgg gcgccgggca aaacgcgttc gggcgtcgcc
     5401 aggccgacca gttctttgtt gacgatcttt cgaatctcct cggagcgctc ccggcccgcg
     5461 ggcgagtgaa ccgccgccag cccccgctcc cgggcccagg cggccagggc tttttcctgt
     5521 tccccccagt tcatccaatc cgtgtcgatg aaaaaccgac ggacctcctc gatccgctcc
     5581 ggggcggggt gcgcgtcgtt gaaaaggaaa cgactccaca gctcggcttt cgtttcgaaa
     5641 gggtatcc
//
LOCUS       DAOQLS010000037         5633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6349_length_5633_cov_3.651847, whole genome shotgun sequence.
ACCESSION   DAOQLS010000037 DAOQLS010000000
VERSION     DAOQLS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6349_length_5633_cov_3.651847"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="PK362_01270"
     CDS             <1..1346
                     /locus_tag="PK362_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC73504.1"
                     /translation="DSLEPASALERATGAAGDWGRRSVRERADRLLGAAREMRRRRFE
                     WTAKIIREVGKTWAEADADVAEAVDFIEYYAAEARRGAVPPPLPSLPGLRNRMVHRPL
                     GAGVVIAPWNFPLAIPCGMTVAALATGNTVVLKPAEQSPGIARDLVNLLAAVCPPGVV
                     QGVYGPGETVGAALVKDPRTRFVAFTGSDETARRIRMAAADATFPKRLILETGGKNAI
                     FVDEDADLDLALAAVIDSAFGYSGQKCSACSRLILHAKIRDRFLPRFLELVAALRVGD
                     PADPSTQLGPLIDDEAFRKVRGFIERGKNEGRLLTPEKWEESRRWVWPAVFELPSPGT
                     PLAREEIFGPVVAVLAAGNASEAFALMNATRFGLTAGVFTRSARTKALFAREVVAGVK
                     YVNRKITHASVGLIPFGGTRASGTGVHAGGPFYLQQFLQQEVQIEEPPHYEIDFP"
     gene            1385..1458
                     /locus_tag="PK362_01275"
     tRNA            1385..1458
                     /locus_tag="PK362_01275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1419..1421,aa:Met,seq:cat)
     gene            complement(1547..2014)
                     /locus_tag="PK362_01280"
     CDS             complement(1547..2014)
                     /locus_tag="PK362_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73505.1"
                     /translation="MFRNFPLFLTTDRRRRFGGALALLAALSGSVGCARPGVNVEMSR
                     WVGQPYARVLYAFGQPAQEKTLPDGRRLVTYEFPHTATIVNQFTNAADRLGATLQNAV
                     GTVQVPRPAKDRYTNGISKRLFIVSADTVEDCVFIGHDGKASPPGKDFKPARP"
     gene            complement(2025..3335)
                     /locus_tag="PK362_01285"
     CDS             complement(2025..3335)
                     /locus_tag="PK362_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNC73506.1"
                     /translation="MIRFKIRCRLALPALLSLAGALRAEPGSLNLGVDYGLRGVATLN
                     PDANGSTSGTLNYYSQSARLSLATELSPGVDVDFRIRSLNVWGLEGSGAPLVRYPAAD
                     GTPWVERAAARLAGLAWNRLTLTLGRQPFLLGDGVLVSDDELGFNGLRADVKLPKNLD
                     LTLFTAKISESLGAGDDSDLILGSLGFSRDINRWELSWLREKTGAASVYQLATGTAAA
                     TDVRRDFIDLRLFGDLKDAYYKLELAFESGEAQIGGANTSIGGSGQRIELGAQTDASR
                     WGRFGVKALYASGSGDDAGTPTKDESFQPSFTRRWDGFERAGFGSFFGASLSDVFNAS
                     APFSNGGSGLPLGASGIKTLGLGLSTVQKVKWTASLDYYLFDSRVKVSGLNSLGSEAD
                     LTLVYRHSGYVTFAAGAAYFFPGDLYGPDASRVSRYTAETQIHF"
     gene            complement(3421..4785)
                     /locus_tag="PK362_01290"
     CDS             complement(3421..4785)
                     /locus_tag="PK362_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73507.1"
                     /translation="MRNWTVALAALALAGSPAFAADALGLKNISTDGSIEVLGVSANN
                     EQDYSDPANDHRGDTVTRVRVGLSADVTEDVKARVEAVRNPSTTSAAQYGNAARPTDL
                     NTEQSAFKFQNAYLDLSNFLSLDSVRLGRQYVGRANDAIVYFGPVNDDLLGVSALDAL
                     AVSKKVGPVTLLGVTGKVSDDDVIATTSTATDAGDTAGDVNASWITASSDELIKLKDL
                     KVPLEIGYYSMTNSNAAATSDNANLSIIDLRAGVQCPKDSFKVMLEYAMNGGQQNAGA
                     TKTKFKGSLLLLTAYWKGEDNAYGVHADFASASGDDTADTNDKSFHDIAGDYRWGEIL
                     SNDNVADVGVGFDSGAQGPGLNVVKIGGHYVLPVVDKKVSLHADYIMAKTSKSVTLAG
                     GTSKKIGNEIDLAVKYKHSDNVMAKVGYAMLSPDDVLTGGGSAQDDQVSKAFAKLLVK
                     WGNN"
     gene            5218..>5633
                     /locus_tag="PK362_01295"
     CDS             5218..>5633
                     /locus_tag="PK362_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73508.1"
                     /translation="MTDKPHSHTPEAGPAKPAAPAAKPPVHAGAGKKKGAEFIKKHKF
                     VAMAGAFIVVGFLMWLVMKLMGGKPAEGPAQNTGSGTIAVNVLEMKPDHFQDMMIAVG
                     SIKGGSEIPLRFEVEGGIDLFEFRQGDKVRKGDIIAR"
BASE COUNT         1052 a         1649 c         1882 g         1050 t
ORIGIN      
        1 cggattccct ggaacccgcc tcggcgttgg agcgggcgac gggcgccgcg ggggattggg
       61 gccgccgttc cgtccgggag cgggcggacc ggctgctcgg cgcggcccgg gaaatgcgcc
      121 gccgccgttt cgaatggacg gccaaaatca ttcgggaagt ggggaaaacc tgggccgaag
      181 ccgacgccga cgtggcggag gccgtggatt tcatcgaata ctacgccgcc gaagcccggc
      241 ggggggcggt cccgccccct ctgccgtccc ttccggggtt gcggaatcga atggtccatc
      301 ggcccctggg cgcgggcgtc gtcatcgccc cgtggaattt ccccctggcc attccctgcg
      361 ggatgaccgt ggcggccctc gcgacgggca acaccgtcgt gttaaagccg gcggaacagt
      421 ccccgggcat cgcccgggac ctggtgaacc tgttggcggc ggtttgtccg cccggggtgg
      481 tgcaaggggt ctacggcccg ggggaaaccg tcggagcggc cctggtgaag gatccccgga
      541 cccgcttcgt cgccttcacg gggtccgacg aaaccgcccg gcgaattcga atggccgccg
      601 ccgacgcgac gttccccaaa cgcttgattt tggagaccgg gggcaagaac gccatttttg
      661 tcgacgaaga cgcggatctg gatttggccc tggccgccgt catcgactcg gccttcggct
      721 acagcgggca gaaatgcagc gcctgttccc ggttgatcct ccacgccaaa atccgggacc
      781 ggtttctgcc gcggtttttg gagctcgtgg cggcccttcg ggtgggggac cccgccgatc
      841 cgtcgactca attggggccg ttgattgacg acgaagcgtt ccggaaagtc cggggcttta
      901 tcgaacgggg gaaaaacgaa gggcggctcc tcaccccgga aaaatgggag gaatcccggc
      961 ggtgggtgtg gcccgccgtg ttcgaattgc cgtcccccgg aacgcccctg gcccgggagg
     1021 aaatcttcgg tcccgtggtc gcggtgctgg cggcggggaa cgcgtccgaa gccttcgccc
     1081 tcatgaacgc cacccggttc gggttgaccg ccggggtgtt cacccgctcc gcgcgaacca
     1141 aggccctctt cgcccgggaa gtggtggcgg gcgtcaaata cgtgaatcga aaaatcaccc
     1201 acgccagcgt cgggctcatc cccttcggcg gaacccgggc ctcgggcacc ggcgtgcacg
     1261 ccggcgggcc cttttacctt cagcaatttc tccaacagga agttcaaatc gaagaaccgc
     1321 cccattacga aatcgatttt ccctgatctt gtcaaagagt cgttaaaaac tgctaaaatc
     1381 catgcgcggg gtggagcagc ctggtagctc gcgaggctca taacctcgag gtcgtaggtt
     1441 caaatcctac ccccgcaaca aaaaaaaccg gtcgcgcatt ggcgcgggcg gtttttttgc
     1501 ttttaagggg gccgatttta gggaaggagc gtttttgggg ggggcgctaa gggcgggcgg
     1561 gtttaaaatc tttgcccggg ggggaggctt ttccgtcgtg gccgataaaa acacaatctt
     1621 cgacggtgtc ggccgaaacg ataaagaggc gtttggagat cccgttggtg tagcggtcct
     1681 tggcggggcg ggggacttga acggttccca cggcgttttg aagggtggcc cccaggcggt
     1741 cggcggcgtt ggtgaattga ttgacgatgg tggccgtgtg gggaaactcg taggtcacca
     1801 ggcggcggcc gtcgggcagg gtcttttcct gggccggttg gccgaaagcg tagagcaccc
     1861 gggcgtaggg ttgaccaacc cagcgcgaca tttccacgtt cacgccgggg cgcgcacatc
     1921 cgaccgaacc cgagagggcc gccagcaacg ccaaggcgcc gccgaaacgg cgccggcggt
     1981 cggtcgttag gaaaagagga aaattccgaa acacggaaaa ggggttagaa gtggatttgg
     2041 gtttcggcgg tgtaccggct cacgcgggaa gcgtcggggc cgtagaggtc gccggggaaa
     2101 aagtaagcgg cgccggccgc gaaggtcacg taaccggaat gccggtagac cagggtcagg
     2161 tccgcttccg accccaggga attgagaccg gacactttga cccgggaatc gaaaaggtag
     2221 taatccaggg acgcggtcca tttcaccttt tggaccgtgg acaaccccag gccgagggtt
     2281 ttaatgccgg aggccccgag gggaaggccc gagcccccgt tggaaaaagg cgccgaggcg
     2341 ttgaacacgt cggacaggga agcgccgaaa aaggatccga atcccgcccg ttcaaaccca
     2401 tcccaccgac gggtaaaaga cggttgaaag ctttcgtcct tggtcggggt gccggcgtcg
     2461 tcccccgagc ccgacgcgta aagggctttc acgccgaacc gcccccaacg ggaggcgtcg
     2521 gtttgggccc ccagttcaat gcgttgcccc gaccccccga tggaggtgtt cgcgccgccg
     2581 atctgggctt cgccgctttc aaaggccaat tccaacttgt agtaggcgtc ttttaagtcg
     2641 ccgaaaagcc gaaggtcgat gaaatcccgc cgaacgtcgg tggccgcggc cgtgcccgtg
     2701 gccagctggt acaccgaagc ggcgccggtt ttttcccgga gccaggacaa ttcccaacga
     2761 ttgatgtcgc gcgagaaccc cagggacccg aggatgaggt cggaatcgtc gcccgccccc
     2821 agggattcgg aaatcttcgc ggtgaaaagg gtgaggtcga ggttcttggg cagttttacg
     2881 tccgcccgga ggccgttaaa gcccaattca tcgtcggaaa caaggacccc gtcgcccaga
     2941 aggaacggtt ggcgtcccag ggtgagggtc aggcggttcc aggcaaggcc ggccaggcgg
     3001 gcggcggccc gctcgaccca gggcgttccg tcggccgcgg ggtagcggac gaggggggct
     3061 cccgaccctt ccaggcccca cacattgagg gagcgaattc gaaagtccac gtccacgccc
     3121 ggcgagagtt ccgtggcgag ggacagacgg gccgattggg aatagtaatt cagggttccg
     3181 gacgtggacc cgttggcgtc ggggttcagg gtggcgacgc cgcgaagacc gtagtcgacg
     3241 cccaggttca gggaaccggg ctcggcccga agggcgccgg ccagggacag gagagccgga
     3301 agcgccaggc ggcagcggat tttgaatcga atcatcaggg ccgattctac cataaaaaga
     3361 aaaacccccg ccccggtaac ccggggcggg ggttttcttt aaagatggtt cagaggcgaa
     3421 ttagttgttg ccccatttga ccaacagttt cgcaaacgct ttcgaaacct ggtcgtcttg
     3481 ggccgagccg ccgcccgtca acacatcgtc gggggagagc atcgcgtaac cgactttcgc
     3541 catcacgttg tcgctgtgtt tgtacttcac ggcgaggtcg atttcgttgc cgatcttttt
     3601 gctcgtgccg ccggcgagag tgaccgattt ggaggtcttc gccatgatgt agtcggcgtg
     3661 gaggctgact ttcttgtcga ccaccggcag gacgtagtgg ccgccgattt tgacgacgtt
     3721 caagccgggg ccctgggctc cggagtcgaa cccgacgccc acatcggcga cgttgtcgtt
     3781 gctcaggatt tcaccccagc ggtagtcgcc ggcgatgtcg tggaacgatt tgtcgttggt
     3841 gtcggccgtg tcgtcgccgg aggcgctggc gaagtcggcg tgaacgccgt aggcgttgtc
     3901 ctcacccttc caataggcgg tcaggaggag gagggagcct ttgaacttgg ttttggtcgc
     3961 gccggcgttc tgctggccgc cgttcatggc gtattccaac atgactttga agctgtcttt
     4021 ggggcactgc acgcccgccc ggaggtcgat gatcgacaga ttggcgttgt cgctcgtcgc
     4081 cgcggcgttg gaattggtca tgctgtaata accgatttcc aggggcactt tgaggtcttt
     4141 gagtttgatc agctcgtccg aggacgcggt gatccaggag gcgttcacgt cgccggcggt
     4201 gtcgcccgcg tcggtggccg tggaggtggt cgcgatgacg tcgtcatcgg agactttgcc
     4261 ggtgacgccg agcagcgtga cgggaccgac tttcttggag acggccaggg cgtccaaggc
     4321 gctgacgccc aggaggtcgt cgttcacggg gccgaagtag acaatggcgt cgttggcgcg
     4381 gccaacgtat tggcggccca ggcgaacgct gtccaggctg aggaagttcg acaagtccaa
     4441 ataagcgttc tggaacttga acgccgattg ttcggtgttg aggtcggtgg ggcgggcggc
     4501 gtttccgtat tgggccgcgg aggtcgtgct ggggttgcgg accgcttcca cgcgcgcttt
     4561 aacgtcctcg gtcacgtcgg cggaaagacc gacgcggaca cgggtgaccg tgtcgccgcg
     4621 atggtcgttg gcggggtcgg aataatcttg ttcgttgttg gccgacacgc ccaacacttc
     4681 gatggagccg tcggtggaga tgttcttcag acccagcgca tcggcagcga acgccgggga
     4741 accggcgagc gccaaagcgg cgagtgccac tgtccagttt ctcatttgtg tgttgcctcc
     4801 ttcgttggag agaatccgac cgcgcttcag cgcgcggtcg aacagctcaa gttcaccgaa
     4861 tcacgatggg gtctccaccg taaggggaca gggagccggc cttgacggcg gagcttcctg
     4921 accccctatg caaaaaccga gcgtcgaccg ccttcggtga gagcggccaa agactcggct
     4981 ttcaagcagg ggacccaacc acacggggta tttctattaa attgtattgg cgctgtcaaa
     5041 tattacaagg gctttctttt caattaatcc ttgaaacccg ggaaccatcg ggtgtataca
     5101 tatatagtcc gccatggaaa tcccggaccg gtacgccgga aggcgaaatt cggatttacc
     5161 ttccgcggcc gtatttggta taactgtcgc aacttattta caccaggatg atcttcattg
     5221 accgataaac cgcattccca cacgcccgaa gccggaccgg cgaaacccgc cgcccccgcc
     5281 gccaagcccc ccgtccacgc cggggccggt aagaagaagg gcgccgaatt cattaaaaag
     5341 cacaagttcg tcgccatggc cggcgctttc atcgtcgtcg gcttcctcat gtggctggtc
     5401 atgaagctca tgggcggcaa acccgccgaa gggcccgccc agaacaccgg cagcggcacc
     5461 atcgccgtga acgtgctcga aatgaaaccc gaccatttcc aggacatgat gatcgccgtg
     5521 ggctcgatca agggcggttc ggaaatccct ttgcgtttcg aagtggaagg cggcatcgac
     5581 ctttttgagt tccgccaggg cgacaaggtg cgcaagggcg acatcatcgc ccg
//
LOCUS       DAOQLS010000038         5517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6604_length_5517_cov_4.493592, whole genome shotgun sequence.
ACCESSION   DAOQLS010000038 DAOQLS010000000
VERSION     DAOQLS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6604_length_5517_cov_4.493592"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK362_01300"
     CDS             complement(<1..270)
                     /locus_tag="PK362_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNC73509.1"
                     /translation="MSKPKPRSTASRVPTRWVIAVDGPAGAGKSSAARGLARTLGFRH
                     IDTGAMYRAAAWAALRAGVPLDAEKALIRVVGKTRFDFTDGATRVD"
     gene            complement(257..1567)
                     /gene="aroA"
                     /locus_tag="PK362_01305"
     CDS             complement(257..1567)
                     /gene="aroA"
                     /locus_tag="PK362_01305"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNC73510.1"
                     /translation="MSISTSSRVVVPGGALRGETRPPPDKSITHRAVILSALAQGTSR
                     IVHPLEADDCERTAEAFRTLGVSIELDKRGEWIVVGRGLYGLAEPSADHIYCGNSGTT
                     IRLLSGVMAGQPFAARLMGDKSLSARPMQRVVDPLLQMGADISARDGRFAPLRIHGRR
                     PLRAIDWKSPVASAQVKSCLLLAGLYAEGVTTFEEPLRSRDHMERMMAAAGVKLTVEE
                     KRVGVQGGQSLKPQEWVVPSDISSAAFFIVAALLTPGSEVMLRNVNINPTRDGVLEVL
                     NKMGASIKQEDVRVVAGEPLADLLVRPAALRATQFGEEILPRLIDEVPILALAATQAQ
                     GTTEIRGAGELRVKESDRLAHVARGLNALGARVEELPDGLRITGPTPLVGATVDSAGD
                     HRLAMTFGVAGLIARGETHIRDADCVDISFPTFWQEFEKLRVQA"
     gene            complement(1571..2431)
                     /locus_tag="PK362_01310"
     CDS             complement(1571..2431)
                     /locus_tag="PK362_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNC73511.1"
                     /translation="MAGPQRVVIVGVGLLGGSLGLALRRRTRARVIGLSHRPGTVLRA
                     RRRGVFHEGHTDPARALPGADVVVFCTPVETIVPLARRFAVHFDPNALVMDVGSVKGP
                     IVDGMASVFREPAGAQFIGAHPMAGSEKSGVENAAADLFVGATCALTPTGAARPRAIR
                     RAADFWRSVGARPIVVAPDVHDRAVALVSHLPHLIADALALTAGRWGATPSERKTLRS
                     LAAGSFRDATRVAGADPDLWRGIFDSNAAPLKRAARDFQRTLGGLVGRRWPRTDLRAA
                     QRFHDEFHGS"
     gene            complement(2448..3467)
                     /gene="aroF"
                     /locus_tag="PK362_01315"
     CDS             complement(2448..3467)
                     /gene="aroF"
                     /locus_tag="PK362_01315"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNC73512.1"
                     /translation="MIVTLKSGAKKKEVQEIVAKIKKLGFTPQLSQGAERVVIGVIGE
                     GNKAIAHKDVFESMPQVELVTPISRPYKLVSRQFKKFDTVIELDGVKIGGKEVVIMAG
                     PCSVDTRENLESTGRELKRAGVQILRGGAFKPRSSPYAFQGLGEAALKLLADVRKATG
                     MPVITEVMDTRQVDLVEQYADILQIGARNAQNFDLLREVGRTRKPVMLKRGMATTIEE
                     WLMAAEYILAKGNTNVMLCERGIRTFETATRFTLDLNAVPVLKHLTHLPVVVDPSHGV
                     GVRDYIVPMARAAIAAGADGVIIECHPNPSTALSDGHQALLPAQMHGIVKELRQVAEA
                     VGRTL"
     gene            complement(3464..4597)
                     /gene="hisC"
                     /locus_tag="PK362_01320"
     CDS             complement(3464..4597)
                     /gene="hisC"
                     /locus_tag="PK362_01320"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNC73513.1"
                     /translation="MVEKSPVPEAAAPFPPRTEVQGFEAYTPGRDMETVKRLYKLRRV
                     IKLASNENPLGPSPKARAAAAAAGPQYHRYPDGFSVTLRQTLARHLGVKFNQVTVGAG
                     SDELIELLARAYLNRGDEIVVSDHAFIRYRMAGEMMGARVVSVPMTRLTHDLEAMAAA
                     VTSRTKFLFIANPNNPTGTYNSHDQLEELLITLPARVVPVIDEAYFEFARARRDYPSA
                     VDFFKAGRNLVVLRTFSKAYGLAGLRLGYAVAPEPIIETVERVRPPFNVSLAAQAAGV
                     AALGDRAHLKRTTTLVAREKKALEKALAALGVATVPSAANFLLIHVAPHRGDELFEAL
                     LRLGVIVRAMDEYGLPEHIRVTIGRPEENRQFLNAFRQARTLL"
     gene            complement(4597..>5517)
                     /gene="pheA"
                     /locus_tag="PK362_01325"
     CDS             complement(4597..>5517)
                     /gene="pheA"
                     /locus_tag="PK362_01325"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNC73514.1"
                     /translation="QILDRLAALNPGPLPTDAMEEIFGTVMNNCRLLQKPLSIAYFGP
                     EATFTHQAAMKHFGRGARYAAAKSITDVFDDVEKGRADYGVVPIENSTEGMVNHTLDM
                     FMESDLVIAAERQDPINHCLLAAPGTKKVKSLSSFPQALAQCRRWLESHYPGVPVHEA
                     ASTADAAAQAALHEGVAAVASPLAAEIYRLKVLGASIQDARNNRTRFLVLGKKIASPS
                     GSGRDKTSILVSLKDRVGALHDLLGTFRQAGLNLTKIESRPSKRKAWEYVFFIDFLGH
                     VAEPRVQKVMRDLKDHSVFLKLLGSYPRGD"
BASE COUNT          884 a         1677 c         1989 g          967 t
ORIGIN      
        1 gtccacccgg gtggcgccgt cggtaaaatc aaatcgtgtt ttcccgacca cgcgaatcag
       61 ggccttttcg gcgtccagcg ggaccccggc gcggagggcc gcccaagccg cggcccggta
      121 catggcgccg gtgtcgatgt ggcggaaccc gagggtccgg gcgaggcccc gggccgcgga
      181 ggattttccc gcgccggcgg gcccgtccac cgcgatcacc cagcgggtcg gaacgcgcga
      241 agccgtcgat cggggtttag gcttggacac gaagcttttc aaattcctgc cagaaggtgg
      301 ggaaggagat gtcgacgcaa tcggcgtccc gaatgtgggt ttccccccgg gcgatgagac
      361 cggcgacgcc gaaggtcatg gccaggcggt ggtcgcccgc cgaatccacg gtggcgccga
      421 cgagcggcgt ggggccggtg atgcggagcc cgtcgggcaa ctcctccacg cgggcgccca
      481 gggcgttgag gccccgggcc acgtgggcca gccggtcgga ttccttgacc cgcagttcgc
      541 ccgccccgcg aatctctgtc gtgccctggg cctgggtggc ggccagggca agaatgggaa
      601 cttcgtcgat caaccggggg aggatttcct cgccgaattg ggtggcgcga agggccgccg
      661 gacgaacgag gagatcggcc aggggttccc cggcgacgac gcggacgtcc tcctgcttga
      721 tggacgcccc cattttgttc aggacttcca ggacgccgtc ccgggtgggg ttgatgttga
      781 cgtttcgcag catcacctcc gagccgggcg tgagcagggc ggcgacgatg aaaaacgccg
      841 cgctggagat gtccgagggg accacccatt cctggggttt gagggattgc ccgccctgca
      901 cgccgacgcg tttttcctcc acggtcagtt tgacgccggc ggcggccatc atgcgttcca
      961 tgtggtcccg ggaacgaagc ggttcttcga aggtggtcac gccttcggcg tacaggcccg
     1021 ccaggaggag acaggatttc acctgggcgc tggccacggg gcttttccaa tcgatggccc
     1081 ggagcgggcg gcggccgtgg atgcgaagcg gcgcgaagcg accgtcgcgc gcggagatgt
     1141 cggcgcccat ttgcaggagc gggtcgacca cgcgttgcat ggggcgggcc gagagcgact
     1201 tgtcgcccat gaggcgggcg gcgaagggtt gtcccgccat gacgcccgag agaagacgga
     1261 tggtggtgcc cgaattgccg cagtaaatgt ggtcggccga cggctcggcc agcccgtaga
     1321 ggccccggcc gacgacgatc cactcgccgc gtttgtccaa ctcgatgctc acccccagcg
     1381 tgcgaaaggc ctcggccgtg cgctcgcagt cgtcggcttc caggggatgg acgatccggg
     1441 acgtcccttg ggccagagcc gaaagaatca cggcgcggtg ggtgatggat ttgtccggcg
     1501 gcggccgggt ttcgccccgc agggcacccc ccggaacgac cacgcggctg gaggtggaaa
     1561 tggacatggt ttaactcccg tgaaattcgt cgtgaaagcg ttgggcggcc cgcaggtcgg
     1621 tccggggcca gcgccgcccc accaggccgc cgagggtccg ctgaaaatcc cgcgccgccc
     1681 gtttcagggg cgcggcgttc gaatcgaaga tgccgcgcca gaggtcgggg tcggccccgg
     1741 ccacccgggt ggcgtcccgg aaactgcccg ccgccaggga ccgaagggtt tttcgctccg
     1801 acggggtcgc gccccaccgg cccgcggtga gcgcgagggc gtcggcgatc aaatggggca
     1861 gatggctgac cagggccacc gcccggtcgt gaacgtccgg cgccacgacg atcggtcggg
     1921 cgcccacgga ccgccaaaaa tcggcggcgc ggcggatcgc tcgggggcgg gcggcgcccg
     1981 tcggcgtcaa cgcgcaggtg gcgcccacga ataaatcggc ggcggcgttt tcgacgccgg
     2041 atttttcgga tccggccatg gggtgggccc cgatgaattg ggccccggcg ggctcgcgga
     2101 acacggacgc cattccatcg acgatgggcc ccttgacgct gcccacgtcc atgaccaggg
     2161 cgttgggatc gaagtggacg gcgaatcgcc gggccagggg cacgatcgtt tcgacgggcg
     2221 tacagaacac gacgacgtcc gcgccgggca gggcccgggc cgggtcggtg tgtccttcgt
     2281 gaaagacgcc gcggcgtcgc gcccgaagga cggtgcccgg gcggtgactg aggccgatca
     2341 cccgggcccg ggtgcggcgg cgcagggcga gccccaggga ccccccgagg agtcccacgc
     2401 cgacgatcac gacccgttgc ggtcccgcca cgacgccggt ttccggttta aagggttcga
     2461 ccgaccgctt cggccacctg ccgaagctcc ttcacgatgc cgtgcatttg ggcgggcagc
     2521 agggcctggt gcccgtcgga gagcgccgtg gaggggttgg gatgacactc gatgatgacg
     2581 ccgtcggcgc cggccgcgat ggccgcccgg gccatgggca cgatgtaatc ccgcacgccg
     2641 accccgtggg acgggtcgac caccacgggc aggtgcgtga ggtgtttgag cacgggcacc
     2701 gcgttcaagt ccagggtgaa ccgggtcgcg gtttcaaagg tgcggatccc gcgttcgcaa
     2761 agcatgacgt tggtgttgcc cttggccaaa atgtattccg cggccatgag ccactcttcg
     2821 atggtcgtgg ccatgccgcg tttcaacatc accggttttc gggtgcggcc gacctcccgc
     2881 agaagatcga agttctgagc gttccgcgcg ccgatctgca gaatgtcggc gtattgttcc
     2941 accaggtcca cctggcgggt gtccatcacc tcggtgatca ccggcatgcc cgtggctttc
     3001 cgcacgtccg ccaggagttt gagggcggct tcccccagcc cctggaaggc gtacggcgac
     3061 gaccggggtt tgaaagcccc gccccgaaga atctggacgc ccgcgcgttt gagttcccgt
     3121 ccggtgcttt caaggttttc ccgtgtgtcg acggagcagg gcccggccat gatcacgact
     3181 tccttgccgc cgattttaac gccgtccaat tcgatgaccg tgtcgaattt cttgaattga
     3241 cggctgacca gcttgtacgg gcgggagatg ggggtgacca gctccacctg gggcatggat
     3301 tcaaagacgt ctttgtgggc gatggctttg ttgccctcgc cgatgacgcc gatgacgacg
     3361 cgctcggccc cttgggacag ttgcggggtg aagccgagtt ttttgatttt ggcgacgatc
     3421 tcttggactt cttttttctt ggcgccgctt ttcagggtga cgatcatagc agggtcctcg
     3481 cttggcgaaa ggcgttgaga aattggcggt tctcctccgg gcggccgatg gtgacccgga
     3541 tgtgttcggg caggccgtat tcgtccatgg cgcggacgat gacgcccagg cggagaagcg
     3601 cttcaaacaa ctcgtcgccc cggtgggggg cgacgtggat caacaaaaag ttggcggcgg
     3661 aagggacggt cgcgacgccg agggcggcca gggctttttc cagcgccttt ttttcgcggg
     3721 ccacgagcgt cgtggtccgt tttaaatggg cgcggtcccc caaggcggcc accccggcgg
     3781 cctgggccgc gagggagacg ttgaagggcg gacgcacccg ctccacggtt tcaatgatgg
     3841 gctccggcgc cacggcgtaa ccgagccgaa ggcccgccag gccgtacgcc ttcgagaacg
     3901 tccggagcac caccagattg cgtccggcct tgaaaaaatc gaccgcgctg ggataatcgc
     3961 ggcgggcccg ggcgaactcg aaataggctt cgtcgatgac ggggaccacg cgcgccggga
     4021 gggtgatcag aagttcctcc agctggtcgt gggagttgta agtgcccgtc ggattgttcg
     4081 ggttcgcgat gaagaggaat ttggtgcggg acgtcacggc cgcggccatg gcttccaaat
     4141 cgtgggtgag gcgggtcatg ggcacggaga ccacccgggc gcccatcatt tcccccgcca
     4201 tgcggtaacg gatgaaggcg tgatcggaca ccacgatttc gtcgccccgg ttcagatagg
     4261 cccgggccag aagttcgatg agctcatcgg aaccggcccc gacggtgacc tggttgaatt
     4321 taacgccgag atggcgggcg agggtttggc ggagcgtcac gctgaacccg tcgggatacc
     4381 ggtggtactg gggtccggcc gccgccgcgg ccgcccgggc cttgggcgac gggcccaacg
     4441 gattttcgtt ggacgccaat ttgatcacgc gccgaagctt gtacaagcgc ttgacggttt
     4501 ccatgtcgcg gccgggggtg tacgcttcga acccctggac ttcggtccgg ggcggaaagg
     4561 gcgcggcggc ttccgggacg ggggattttt cgaccatcag tccccccggg ggtaactgcc
     4621 caggagtttt aaaaagacgc tgtggtcttt gaggtcccgc atgacttttt gtacccgggg
     4681 ttcggccacg tggcccagga aatcgatgaa gaacacgtat tcccaggcct tgcgcttgga
     4741 cggccgggat tcgatctttg tcaggttgag ccccgcctgg cggaaggtgc ccaaaagatc
     4801 gtgcagggcg ccgacccggt ctttgaggga aacgaggatg gaggttttgt cccggccgga
     4861 acccgagggg ctcgcgattt ttttgccgag aacgaggaaa cgggtccggt tgttgcgggc
     4921 gtcttggatc gacgccccca gaaccttcag gcggtagatt tccgccgcca gggggctcgc
     4981 caccgcggcc acgccttcgt gcagggcggc ctgggccgcg gcgtcggcgg tggaggcggc
     5041 ttcgtggacg ggaacccccg ggtaatggct ttccaaccag cgccggcatt gggccagggc
     5101 ctgggggaag gaggaaagac ttttcacttt tttggtgccg ggggccgcca ggaggcaatg
     5161 gttgatgggg tcctggcgtt ccgcggcgat gacgagatcg gattccatga acatgtccag
     5221 ggtgtggttc accatgcctt cggtggaatt ctcaatggga acgaccccgt agtcggcgcg
     5281 gcctttttcc acgtcgtcga acacgtcggt gatcgatttg gccgccgcgt agcgggcccc
     5341 gcgcccgaaa tgtttcatgg cggcctggtg ggtgaaggtg gcttcggggc cgaaataggc
     5401 gatggaaagg ggtttttgga ggaggcggca gttgttcatc accgtcccga aaatttcctc
     5461 catggcgtcg gtgggaagcg gtccggggtt gagggcggcc aggcggtcca ggatttg
//
LOCUS       DAOQLS010000039         5496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6646_length_5496_cov_3.769528, whole genome shotgun sequence.
ACCESSION   DAOQLS010000039 DAOQLS010000000
VERSION     DAOQLS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6646_length_5496_cov_3.769528"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1355
                     /locus_tag="PK362_01330"
     CDS             111..1355
                     /locus_tag="PK362_01330"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNC73515.1"
                     /translation="MTPPTLWDQALAQLDRGAQRLGLEPYIVERLRHPKRILEVSVPV
                     RRDDGSVKVFRGYRVQHNVDRGPAKGGIRYHPDVSLDDVAALAFWMTMKCAAVNLPYG
                     GAKGGVVCDPKTLSEGELERLTRRYASEISILIGPDKDIPAPDMNTNEKIMGWIMDTY
                     SSQVGHSVPGVVTGKPVEIGGTLGRREATGRGVFFIVQELARVKNWQLKDLRIAVQGF
                     GNVGSNAARILREAGARVVAISDATGGVADPAGVDVDDARRHCEKETLATYRKGRPLT
                     NRELLELDCDVLIPAALENQITAEVAPRVKARFIVEGANGPTTTEADRLLNERGVTVV
                     PDILANAGGVTVSYFEWVQDLQAFFWDEGEVRDRLRAILLRAFQDVWRVAGENKTDLR
                     LAAFLVALDRLAKAIRQRGLFP"
     gene            1391..2071
                     /locus_tag="PK362_01335"
     CDS             1391..2071
                     /locus_tag="PK362_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73516.1"
                     /translation="MERLLPIWNTYVELMGDPTFAIASALAVFGLMVFLALMVKFFKA
                     EKAPDLNSLSGGVDPSALTPPKPVKDAPKADPPVPASTATTKLTPPPELAATMVLPPG
                     ELPRPAAPPPPGSVDVAMYELLVRRVASLEGEVKKDPLFLDPLLKRLSLMEKRLEEIG
                     ELSKKIDELAKTVAQAPAPAAPATGESGPASAGGIDPAEFHAFKEKVYGLQKILEHLA
                     ESPGTPPA"
     gene            2068..2631
                     /locus_tag="PK362_01340"
     CDS             2068..2631
                     /locus_tag="PK362_01340"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73517.1"
                     /translation="MTRSRILLLMLLDLVLMGGAGYRAFERYGELKTSLPTAAVAPPP
                     ASPVETPAVETSTAPAVPTETTALSTATAEATIPAPSTSTVPVSAASTAPAVALSTAP
                     VHAARVAFRYRNSVAKKVFLTGTFNNWTPTAFRKSASGLWTLVVDIAPGDHAYNFVVD
                     GKTVRDPNQRRSDEKGRSLLRVAPPSK"
     gene            2699..>5496
                     /locus_tag="PK362_01345"
     CDS             2699..>5496
                     /locus_tag="PK362_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC73518.1"
                     /translation="MASANDYISVNASVPRWIGPAFFRCLALAVSGILPLSSTPVSAK
                     ESEPIRYFQFKFKPDQPAESLQNYLTFVQKNAPGIEGLRTQYAVADAYMAQGKWDEAA
                     EILQTIVQVPQADEFFRASVLIKLADCNMRMAQFAAASGFYTAAAQSAVRSILPEATI
                     GLALACVAKGEREKAFVHFQELIAFYPSYKIHPRYMFPLGLIQWEVRKYEGALDYFLK
                     DEGNPASLYFAGLCYRELKRVPEATAVFRRITEDHPESIWANRARFEVGETFYQQKDF
                     NLAAQTFQNIYRDRPSDQWETLAYYRMACVDMQQNNFKAAEEKLTILADDNKDPMLLS
                     NITYLLSEALAEQKKVTRLIDILESTQKTKRKSVDNMYRLIWGYTAQGDYKKAIDLAN
                     DFLSTQLDADLTPRALLVQGYAFNKLGKLPEAFASYQLVVENFPATPYAAKATQLAAM
                     NYYRAGQFKAVTTQVNSLWESIDNGVRNHYPETLYWLAQAHLKLRHGDEAESLFKEFA
                     KVAPPRHPWLPQALRAQALASALGKSSETALPILQRAYQNASDIGDRALMGKLTLDMA
                     NISFNAKKYEKAAGFYRQMEQVDPKNEKLPFALFQGGVALYRSSYYTDAIDNWMKLVR
                     QFPKDARAPDALFRASKTWFDLAKFSEAIAGFEDLMRNYPLTDFAKDSLLQIGQAYYN
                     DKNFTKAIDTYKDFLTKYPRDPQSVQVAQLLQMAYYKTGKSPEDIDALTKGQTKNPVL
                     VDVYWEEGAKLYNEKDYPKARELFEKIIFNFPTSSLAPQASFYRSEALFLEEKYQEAA
                     VAYEGFLQSYKDDAQRNLAMFHLAVSRYNLKQFPEAAQTFELFAQTFPKDPLAQNASL
                     NSALSYIQAQNVDEAVDAVQSYATAFPNAEDLGTAYLQLGQFLEKLGQEQKAAEIYRQ
                     TPPQRAEYPEALYNVARV"
BASE COUNT         1163 a         1817 c         1496 g         1020 t
ORIGIN      
        1 cagcggtgcc aaagcccctg gacctgcccc cacgggcgtc ccacgtttct ccgtttgacg
       61 aacgaagacc tggcaaaacg attccgccgg atgtagagtt aagcgtcacc atgacgccac
      121 cgactctgtg ggatcaagcc ctcgcgcaat tggaccgcgg cgcccaacgc ctcggcctcg
      181 aaccctacat cgtcgaacgc ctccgccacc ccaaacgcat tctggaagtg tccgtgcccg
      241 ttcgccggga cgacggctcc gtgaaggttt ttcgcggcta ccgggtgcaa cacaacgtcg
      301 accggggccc ggccaagggc ggtatccgct atcaccccga tgtgtcgctg gacgacgtcg
      361 cggccctggc gttttggatg acgatgaaat gcgcggccgt caacctgccc tacggggggg
      421 ccaagggggg ggtcgtgtgc gatcccaaaa ccctttccga gggggaactg gaacgattga
      481 cccgacgcta cgcctcggaa atttccatat tgatcggtcc cgacaaggac atccccgccc
      541 ccgacatgaa caccaacgaa aaaatcatgg ggtggatcat ggacacctat tcctcccagg
      601 tcggccattc ggtccccggt gtggtcacgg ggaagcccgt ggaaatcggc ggaacgttgg
      661 ggcgtcggga agccacgggg cggggggttt tcttcattgt ccaggaattg gcccgggtga
      721 agaattggca actcaaggat ctccgcattg ccgtccaggg cttcggcaac gtcggctcca
      781 acgccgcgcg cattctccgg gaagccgggg cccgcgtcgt tgcgatttcg gacgccacgg
      841 gcggcgtggc ggacccggcc ggcgtggacg tggacgacgc ccgacgacac tgcgagaaag
      901 aaacgttggc gacctatcga aagggtcgcc ccctcacaaa ccgggaactc ttggaattgg
      961 attgcgatgt gctgatcccc gcggccctgg aaaaccaaat cacggcggaa gtcgctcccc
     1021 gggtgaaggc ccggttcatc gtggaaggcg ccaacgggcc gacgaccacc gaggccgacc
     1081 gcctcctcaa cgagcggggg gtcaccgtgg tgcccgacat cctggccaac gccggcggcg
     1141 taaccgtcag ttattttgaa tgggtccagg atctgcaggc ttttttctgg gacgaggggg
     1201 aagtccggga ccgcctccgg gccatcctcc tacgggcctt ccaggacgtc tggcgggtgg
     1261 cgggggagaa caaaaccgat cttcggctgg ccgccttttt ggtggccttg gaccgattgg
     1321 ccaaggcgat acgtcaacgg gggctgtttc catgattgac aagcccatcc aattccttta
     1381 aattctaaaa atggaacgcc tccttccgat ctggaacacg tacgtggagc tcatgggcga
     1441 cccgaccttt gccatcgcct cggccctggc cgtgtttggg ttgatggttt ttctggcgtt
     1501 gatggtcaaa tttttcaagg cggaaaaggc gccggatttg aactccctct cggggggggt
     1561 ggacccgtcg gccctgaccc cgcccaagcc cgtcaaagac gcccccaagg ccgacccccc
     1621 ggttccggcg tccaccgcca ccacgaaatt gactccgccg cccgagctcg ccgccacgat
     1681 ggttttgccg ccgggcgaat tgccccgccc cgccgcgccc ccgccccccg gttcggtgga
     1741 cgtggccatg tacgaattgc tcgtccgtcg ggtggcttcc ctggaaggcg aagtcaagaa
     1801 agaccctttg tttctggatc cgcttctgaa gcgtctaagc ctcatggaaa aacgccttga
     1861 ggaaatcggc gaattgtcga agaaaatcga cgaactggcg aaaacggtgg cccaggcccc
     1921 ggcgcccgcc gccccggcga cgggggaatc cggcccggcg tccgccggcg ggatcgaccc
     1981 ggccgaattt cacgccttta aggaaaaagt ctacggcctt caaaaaatcc tcgagcatct
     2041 ggccgaatcc cccggaaccc cgcccgcatg acgcgctccc gaatccttct tttaatgctg
     2101 ttggatcttg ttttgatggg cggggccggc tatcgggcct ttgagcgtta cggggagttg
     2161 aaaacctctt tgccaacggc cgccgtcgcg ccgccgccgg cctccccggt ggaaacgccc
     2221 gccgtggaaa cctccaccgc cccggcggtc ccgacggaga cgacggcgct ctccaccgcc
     2281 acggcggaag cgacgattcc ggcgccttcc acctcgaccg tgcccgtttc cgcggcgtca
     2341 acggccccgg cggtggccct gtcgacggcc cccgttcacg ccgcccgggt ggccttccga
     2401 tatcgtaatt ccgtcgcgaa gaaggtcttt ctcaccggaa ccttcaacaa ttggaccccg
     2461 accgccttcc gaaaatccgc ctcgggactc tggactctcg tcgtggacat cgcccccggg
     2521 gaccacgcct ataatttcgt ggtggatggg aaaaccgtcc gcgatccgaa ccaacgccga
     2581 agcgacgaga aggggcgatc tttgcttcgc gtcgccccgc cctccaagta accgccgctc
     2641 ctgaaaaaat tctgtaacgg tattgcaaat tcgcccctcg gaccctataa ttaagagaat
     2701 ggcttccgcc aacgattaca tctcagtgaa cgcctccgtc ccgcggtgga tcgggcccgc
     2761 gtttttccgt tgtttggcct tggccgtttc cggaatcctt ccgttgtcct ccacgccggt
     2821 ctcggccaag gaatcggaac ccatccggta ttttcaattt aaattcaaac ccgaccaacc
     2881 ggccgaatcc ctccaaaatt acctgacctt cgtccaaaaa aacgccccgg gcattgaagg
     2941 cctccgcacc caatacgccg tcgccgacgc ctacatggcc caggggaaat gggacgaggc
     3001 cgcggaaatc cttcaaacca tcgtccaagt gccccaggcc gacgaatttt tccgggccag
     3061 cgtcttgatc aaattggccg actgcaacat gcgcatggcc caattcgcgg cggccagcgg
     3121 tttttacacc gcggccgccc agtccgccgt gcgttcgatt cttccggagg ccaccatcgg
     3181 cctggccctc gcctgcgtgg cgaagggaga gcgggaaaaa gccttcgttc acttccagga
     3241 actgatcgct ttctatccct cctacaaaat ccacccccgc tacatgtttc ccctcgggct
     3301 gattcaatgg gaggtgcgca aatacgaggg ggccctggat tactttttga aggacgaagg
     3361 gaacccggcc agcctttatt tcgccggcct ctgctaccgg gaactcaagc gcgtgccgga
     3421 agccaccgcc gttttccgcc gaatcaccga agaccatccg gaatccattt gggcgaaccg
     3481 cgcccgcttc gaggtggggg aaacctttta ccaacagaag gattttaatc tcgcggccca
     3541 aaccttccag aacatctacc gggaccgccc ctcggaccaa tgggaaaccc tcgcctacta
     3601 ccggatggcc tgcgtcgaca tgcaacaaaa caatttcaag gcggccgagg aaaaactcac
     3661 gatcctggcg gacgacaaca aagaccccat gttgctgtcc aacatcacct acttgctctc
     3721 cgaagccctg gccgaacaaa agaaggtgac ccgattgatc gacattttgg aatcgaccca
     3781 aaaaaccaag cgaaaatccg tggacaacat gtaccggctc atttggggtt ataccgccca
     3841 gggggattac aagaaggcca tcgatctggc caacgatttt ctgagcaccc aactggacgc
     3901 cgatttgacc ccccgggctc ttttggtgca gggctacgcc tttaacaagt tggggaaatt
     3961 gcccgaggcc ttcgcgagtt atcagctggt cgtcgaaaac tttccggcca cgccctacgc
     4021 cgccaaagcc acccaattgg ccgccatgaa ttactaccgc gccggtcaat tcaaggcggt
     4081 caccacccag gtcaacagtc tttgggaatc catcgacaac ggcgttcgaa accattaccc
     4141 ggaaacgctc tattggctgg cccaggccca tttgaaattg cgccacgggg acgaggccga
     4201 aagcctgttc aaggaattcg ccaaagtcgc cccgccgcgc cacccctggc tgccccaggc
     4261 ccttcgagcc caggccctgg cgagcgccct gggaaaaagc tccgaaaccg ccctccccat
     4321 tcttcaacgg gcctaccaga acgccagcga catcggcgat cgggccctca tgggcaagtt
     4381 gaccttggac atggcgaaca tttccttcaa cgcgaagaaa tacgaaaagg ccgcgggctt
     4441 ttaccgccaa atggaacagg tggacccgaa aaacgaaaaa ctgcccttcg ccctctttca
     4501 aggcggggtg gccttgtatc gctcgtccta ctacaccgac gccatcgaca attggatgaa
     4561 actcgtccgt caattcccca aggacgcccg ggcccccgac gccctgttcc gggcctccaa
     4621 gacgtggttc gatttggcca aattctccga ggccatcgcg gggttcgaag acctgatgcg
     4681 aaattacccg ttgacggatt tcgccaagga ttccctgctt caaatcggtc aagcctatta
     4741 caacgacaag aatttcacca aagccatcga cacctacaag gatttcctga ccaaataccc
     4801 ccgggatccc cagtccgtcc aagtggcgca gcttcttcaa atggcctact acaaaaccgg
     4861 caagtccccc gaggacatcg acgcgctcac gaaaggacaa accaagaacc cggtgctggt
     4921 ggacgtttac tgggaagagg gggccaagct ttacaacgaa aaggattacc ccaaggcccg
     4981 ggaacttttc gaaaagatca tcttcaactt ccccacgtcc tccctggcgc cccaggcctc
     5041 tttttaccgt tcggaagccc tctttttgga ggagaaatac caggaagccg ccgtcgccta
     5101 cgagggcttt ttacagtcct acaaagacga cgcccagcgc aatttggcca tgttccacct
     5161 ggccgtcagc cgttacaatt tgaagcaatt ccccgaggcg gcccaaacct ttgagctctt
     5221 cgcccaaacc ttccccaagg atcccctggc ccaaaacgcc tcgctgaact ccgccttgtc
     5281 ctacattcaa gcgcagaacg tcgacgaagc cgtcgacgcg gtgcaaagtt acgccacggc
     5341 cttccccaac gccgaagacc tcggcaccgc ctacttgcaa ttgggtcaat ttttggaaaa
     5401 actgggccag gagcaaaaag ccgccgaaat ataccgccag acgccgcccc agcgggccga
     5461 gtatcccgag gccctttaca acgtggcccg cgtctt
//
LOCUS       DAOQLS010000040         5474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6693_length_5474_cov_4.713600, whole genome shotgun sequence.
ACCESSION   DAOQLS010000040 DAOQLS010000000
VERSION     DAOQLS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6693_length_5474_cov_4.713600"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PK362_01350"
     CDS             <1..503
                     /locus_tag="PK362_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73519.1"
                     /translation="KDVEGRRLRVRYEKARHWPTLNFNGNYYTDRPAFYDAIDWDAKL
                     TLAAPIYQGGTVAARTAEAVSELRQSEDELARLRRWAQTDVAQRHALWSAALDEVDAH
                     RQAFEAADKSFRRHEEEYRLGLVTNLDVLQALNDRLAMKKAWDDARLNARRAYWDLRA
                     AVGSLP"
     gene            500..3631
                     /locus_tag="PK362_01355"
     CDS             500..3631
                     /locus_tag="PK362_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC73520.1"
                     /translation="MRLSDTAIKNPVFAWMLMAGLILFGWIGFTGIGKSQLPDVDFPV
                     IMVSTRWEGASPEVIETDVVDLIEDALTSVKGLKKITSTSRQGQTMVILEFDLKTDVD
                     VALQETQSKLSQIQATLPKDMEPPTIAKFNPEDQPFMWLSLSGDRPLREMNAYVKDEL
                     KNQFTTIPGVGDVMLGGYVEPSLRVWLDPVRLKAVELTVDDVVAAIQSEHAERPAGYL
                     TSDKQEWNLRVMGEATGVEDFGNLVIPARSGAPLTRIFRLKDVATIEDGLADPRAISR
                     VDGHPAIGLGIRKQRNANTVEVGNRVKKKVAELQSSLPPGMKLEVNFDGTFFVEQSTH
                     ELNFNLILAAVLTSLICWMFLGSWSSAINIILAIPTSILGTFIVLYLLGFTQNTFTLL
                     ALTLVIGIVVDDAIMVLENIVRHREMGEPKVKAAILGAREIYFAAMAASVAILGIFVP
                     VVFVKGVIGKYFFQFGITISAAVMFSLLEALTLAPMRTSQFLEVGHTNAVGRWVDRAM
                     ARSTAAYRRALEFCLRHRWKVVGASLALFIGSLSLGGIVKKELMPPQDQNMLFLRVET
                     PVGSSLAFTDRTLKQVEDFAMKRGEMARYYAAVGGFEGGEINTAFMFITLKDPKDRPV
                     PPGRRKPFSQQELMTEFRNAVKPMPGVESVILQDFSVGGMGADSGFPIEFTLRGRDWD
                     ELGRVSDEVKKKMEDSGLMVDVDTNFDLGMPEVQIFPNREAAALRGVSVSAIGNAINA
                     TLGGMKVGKFSRGGRRYDVRVRFSEGDRTRPSDVDKIWVRNNRGQVIPLREVVTVREK
                     KTLLSITRQDRSRAVTVTANVAPGKSQGDALNKVQEIGRSLPNGVKLVFSGAAETFKE
                     AFQGLALAILLGVVVAYMILGTQFNSFIHPVLVLIALPFSVTGALLALVLGHQSLNMF
                     SFIGLILLMGIVKKNSIMLVDFTNERRKSMPLREALLDACPVRLRPIVMTSVSTIAAA
                     IPPALGLGAGAETRIPMALVIIGGVTVSTFLTLFVVPCAYSLMARLESHRHDRDLKDA
                     LRELGELKTEAPQ"
     gene            3628..4854
                     /locus_tag="PK362_01360"
     CDS             3628..4854
                     /locus_tag="PK362_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNC73521.1"
                     /translation="MNPLGVAVLAALVLVQVIEWTGDALNLRALSPRPPDMFKDLYDA
                     DRYARSQEYTRARIRFGFVVQFLELAGLVGFWFLGGFGVLERWLSPVTPALTRGLLYV
                     GILAAAKDLLSIPFDLYSAFVIEERFGFNRTTLKTFVTDRLKGYGLAVVLGGPVLALV
                     LWLFGRLGSAAWIWGWGAVTAFSLLVQFIAPTWILPLFNKFTPLPEGELRDAIFALAR
                     RLQFPLTNVFVMDGSRRSAKGNAFFTGFGRNKRIALFDTLVEKNTVPELVAVLAHEIG
                     HYKKGHVWKGFALGVAQTGVMFFLLSLALGWGALFQAFGLSAPSAHAGLVVFGILFSP
                     ASFLLGLPLKAYSRRNEFEADRYAKENLGSGGDLQTGLQKLSASSLSNLTPHPFYVFL
                     HYTHPPLTERVGALRS"
     gene            complement(4890..>5474)
                     /locus_tag="PK362_01365"
     CDS             complement(4890..>5474)
                     /locus_tag="PK362_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73522.1"
                     /translation="HRVAVLGLGQPSQLGDADLRIGARGLRIGVGATVARARRCIARV
                     LAGLLALAFTGCNYDFPLTDRPTDRVHKELLGAWTAEEGQERVEIRAFDERSYVVNDN
                     GQLYRMIHTDLGDLRILSVQELESADRRYVYLRWRLTPDGKELHLNGVNTRVIPTSLP
                     SARAVRRAMKKNRSNPDLFTEEGVYTRADAAPTR"
BASE COUNT          972 a         1725 c         1680 g         1097 t
ORIGIN      
        1 aaaaggacgt ggaaggacgg cggttgcgcg ttcgctacga aaaggcccgc cactggccca
       61 ccctcaactt taacggcaac tactacaccg atcgcccggc cttttacgac gccatcgatt
      121 gggacgccaa gttgacgttg gcggcgccga tttaccaggg gggcacggtc gccgcccgga
      181 ccgccgaagc cgtgtccgag cttcgccagt ccgaggacga actggcgcgg ttgcgccgct
      241 gggcccaaac ggacgtggcc caacgccacg ccctgtggag cgcggccctg gacgaagtgg
      301 acgcccaccg ccaggccttc gaggcggcgg acaaaagctt ccgccgccac gaggaggaat
      361 accgcctggg gctggtcacc aacctcgatg tccttcaggc gctcaacgat cgcttggcca
      421 tgaaaaaagc ctgggacgac gcccgcttga acgcccgccg ggcctactgg gacctgcgcg
      481 ccgccgtggg gagcctgcca tgagactgtc cgacaccgcg attaaaaacc ccgtcttcgc
      541 ctggatgttg atggcggggc tgatcctctt cggttggatt ggatttacgg ggatcggtaa
      601 aagccaattg ccggacgtgg attttcccgt cattatggtg tccacccggt gggagggcgc
      661 ctccccggaa gtcatcgaaa ccgacgtcgt cgatctgatc gaagacgcgc tcacctccgt
      721 caaaggcctc aaaaaaatca cctccaccag ccgccagggt cagaccatgg tgatcctgga
      781 attcgacctc aagaccgacg tggacgtggc cctccaggaa acccaaagca agctctccca
      841 aatccaggcg accttgccca aggacatgga gccgccgacg atcgcgaaat tcaaccccga
      901 agaccagccc ttcatgtggc tgtcgttgtc cggcgaccgt cccctgcggg aaatgaacgc
      961 ctacgttaag gacgaactca aaaatcaatt taccaccatc cccggcgtcg gggacgtgat
     1021 gttgggcggt tacgtggaac cgtccctgcg cgtttggctg gaccccgtcc gcctgaaagc
     1081 cgtggaactc accgtcgacg acgtggtggc ggccatccaa tcggaacacg ccgaacgccc
     1141 ggccggctat ctgacgtcgg acaagcagga atggaacctc cgggtcatgg gcgaggccac
     1201 cggggtggaa gattttggaa acctcgtgat tcccgcccga agcggcgcgc cgctcacccg
     1261 aattttccgt ttgaaggacg tcgcgaccat cgaggacgga ttggccgatc cccgggccat
     1321 ttcccgggtg gacggtcacc cggccatcgg gttgggtatc cgcaaacaac gaaacgccaa
     1381 caccgtggaa gtcgggaatc gggtcaagaa gaaagtggcg gagcttcaat cctccctgcc
     1441 gcccggcatg aagctggagg tgaatttcga cggcacgttt tttgtcgagc aatccaccca
     1501 cgagctgaac ttcaatttga tcctggcggc cgtcctcacg tccctgattt gctggatgtt
     1561 cctggggtcc tggagttcgg ccatcaatat catcctggcg atccccacgt cgatcctggg
     1621 gaccttcatc gtcctgtact tgctcggctt cacccaaaac accttcaccc tgctggccct
     1681 gacgctggtg atcggcatcg tcgtggacga cgcgatcatg gtgctggaaa acatcgtgcg
     1741 tcatcgggag atgggggaac ccaaggtcaa ggccgccatt ctgggggccc gggaaatcta
     1801 tttcgccgcc atggcggcct cggtcgcgat tttgggcatc tttgtgcccg tggttttcgt
     1861 gaagggcgtt atcggaaaat acttctttca attcggcatc acgatttccg ccgccgtgat
     1921 gttctccctt ctggaagccc tgacgttggc ccccatgcgg acgtcgcaat ttttggaggt
     1981 gggtcacacc aacgcggtcg gccgctgggt ggaccgcgcc atggcgcgct ccaccgccgc
     2041 ctaccgccgc gccctggaat tctgcctccg ccaccgctgg aaagtcgtgg gggcttcgct
     2101 ggccctcttt atcggctccc tgtccctggg gggcatcgtg aagaaggaat tgatgccgcc
     2161 ccaggaccag aacatgcttt tcctccgcgt cgaaaccccc gtggggtcgt cgttggcctt
     2221 cacggaccgc acgctcaagc aggtcgagga tttcgccatg aaacgcggtg aaatggcccg
     2281 ctattacgcg gccgtcggcg gattcgaagg cggcgaaatc aacaccgcct tcatgttcat
     2341 cactctgaaa gatcccaaag accgccccgt gcccccgggc cgccggaaac ctttctccca
     2401 acaggaactg atgaccgaat tccgcaacgc ggtcaaaccg atgccgggcg tcgaatccgt
     2461 cattcttcag gatttctccg tgggcggcat gggggccgat tcggggttcc ccatcgagtt
     2521 caccctgcgc ggccgggact gggacgaact gggtcgggtg agcgacgagg tcaaaaagaa
     2581 aatggaagac tcgggcctca tggtcgacgt ggacaccaat tttgatttgg gcatgccgga
     2641 agttcagatc ttccccaacc gggaagcggc cgcccttcgg ggcgtcagtg tttccgccat
     2701 cggcaacgcc atcaacgcga cgctgggcgg catgaaagtc ggaaaattct cccggggcgg
     2761 ccggcgttac gacgttcggg tccgcttcag cgagggggac cgaacccggc cctccgacgt
     2821 ggacaaaatt tgggtccgca acaaccgggg ccaggtaata cccctgcggg aggtcgtcac
     2881 cgtgcgggag aaaaagaccc tcctgagcat cacccgtcag gaccggtccc gagccgtgac
     2941 ggtcacggcc aacgtggccc cggggaaatc ccagggggac gcgctgaaca aagtccaaga
     3001 gatcggaaga tccttgccga acggcgttaa gcttgttttc tccggcgccg cggaaacctt
     3061 caaggaagcg tttcagggcc tcgccctggc gatcctcttg ggtgtcgtcg tggcttacat
     3121 gatcctcggc acccaattca acagtttcat ccatccggtg ctcgtgttga tcgccctgcc
     3181 gttcagcgtc accggcgcct tgctggccct ggtgctcgga caccaatccc tcaacatgtt
     3241 cagttttatc ggtctgatcc tcctgatggg catcgtgaag aagaactcca tcatgttggt
     3301 cgatttcacc aacgagcgtc ggaaatccat gccgcttcgg gaagcgctcc tggacgcctg
     3361 cccggtgcgt ctgcggccga tcgtcatgac gtcggtgtcg accatcgccg cggccattcc
     3421 gccggccctg ggtttgggcg ccggggcgga aacgcggatc cccatggctc tcgtcatcat
     3481 cggcggcgtc accgtgtcga ccttcttgac gttgttcgtc gtcccctgcg cctacagcct
     3541 catggcgcgc ctggaaagcc atcggcacga ccgggacttg aaggacgctc ttcgagagtt
     3601 gggcgagttg aaaaccgagg ccccgcagtg aatcccctgg gcgtggcggt gttggccgcg
     3661 ttggtcttgg tgcaagtcat cgagtggacg ggcgacgccc tgaatttgcg cgccttgtct
     3721 ccccggccgc cggacatgtt caaggacctc tacgacgccg accgctacgc ccgcagccag
     3781 gaatacaccc gggcccggat tcgcttcggg tttgtggttc aattcctgga attggcgggg
     3841 ttggtggggt tctggttttt gggcgggttc ggggttctgg agcgttggtt atcgcccgtc
     3901 acgcccgccc tgacgcgggg attgctctac gtggggattc tggcggcggc caaagacttg
     3961 ttgtcgatcc ccttcgacct ctattccgcc ttcgtcattg aagagagatt cggtttcaac
     4021 cggacgacgc taaagacctt tgtcacggac cggctcaaag ggtacggtct cgccgtggtg
     4081 ctgggggggc cggtgctggc cctggtgctt tggctcttcg ggcgattggg gtccgcggcc
     4141 tggatttggg ggtggggcgc cgtcacggcc ttttccctgc tcgttcagtt catcgccccc
     4201 acctggattc tgccgctgtt caacaaattc acgcccttgc cggaggggga actccgcgac
     4261 gccatttttg cgctggcccg ccgtctgcaa tttccgctga ccaacgtgtt cgtcatggac
     4321 ggttcccgcc gatcggccaa aggcaacgct tttttcaccg gcttcggccg gaacaaacgc
     4381 atcgcgttgt tcgacactct cgttgaaaag aacaccgttc ccgagttggt cgccgtcctg
     4441 gcccacgaaa ttgggcatta caagaaaggc cacgtttgga aaggttttgc tttgggagtg
     4501 gcccagacgg gggtgatgtt ctttttgctg tccttggctc tgggatgggg ggcgttgttt
     4561 caggcgttcg ggttgtccgc gcccagcgcg cacgcggggt tggtggtctt cggaattcta
     4621 ttttctccgg cgtcgttcct gctcgggttg cccctcaagg cttattcgcg tcggaacgaa
     4681 ttcgaggccg accgatacgc caaggagaat ctggggtccg ggggggattt gcagaccggt
     4741 cttcagaaac tttcggcttc cagcctgtcc aacctcacgc cccacccctt ctacgtcttc
     4801 ctgcattaca cccacccgcc gttaacggag cgggtggggg cccttcggtc ctaatccgcc
     4861 ttcgcccatt ccgctccccg gacccgccct cagcgggtcg gggccgcgtc cgcccgggtg
     4921 tagacgccct cttccgtgaa gagatcgggg ttcgaacgat ttttcttcat cgcccgccga
     4981 accgcccgcg cggagggaag cgaggtcggg atcacccgcg tgttgacgcc gttcaggtgc
     5041 aattccttcc catcgggggt cagccgccag cgcagataca cgtaacgccg gtcggccgat
     5101 tcgagttcct gaacgctcag gattcggaga tccccgagat ccgtgtggat cattcggtag
     5161 agttggccgt tgtcgttgac cacgtacgac cgttcgtcaa aagcccgaat ctcgacgcgc
     5221 tcctggcctt cctcggccgt ccaggctccc aacagctcct tgtggacgcg gtccgtgggt
     5281 cggtccgtca gggggaaatc gtagttgcac ccggtgaagg ccagggccag gaggccggcc
     5341 aaaacgcggg cgatgcatcg gcgggctcga gcgacggtgg caccgacacc gatccgcagg
     5401 ccgcgtgcgc cgatccgcag gtcggcgtca ccgagctgcg acggctgacc caggccgagt
     5461 accgcaacac ggtg
//
LOCUS       DAOQLS010000041         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6722_length_5459_cov_5.028497, whole genome shotgun sequence.
ACCESSION   DAOQLS010000041 DAOQLS010000000
VERSION     DAOQLS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6722_length_5459_cov_5.028497"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PK362_01370"
     CDS             complement(<1..531)
                     /locus_tag="PK362_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73523.1"
                     /translation="MRRWTVVSLWLAAVTVSAQETPAALSLGDCFRRAAAKSETLALA
                     ESDIRQVENQYALVRAGVLPQAGFRASETWQDAGGRSDASGGGSRVRRSESAFYGRQV
                     LFSGFREFAGMRAQSALGDSRRWGLVRARQLLYGDVAEVFYTVLAYDADRSALTTLGE
                     LTDQRIADLTRRVRIGR"
     gene            complement(528..1217)
                     /locus_tag="PK362_01375"
     CDS             complement(528..1217)
                     /locus_tag="PK362_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC73524.1"
                     /translation="MTDRSVNSAVAARSRSPRSVPIPLVRPPTSLRRRPHARRPAAET
                     RDAILAAAGRALETRDYHALTMDEIARRSGIAKGSLYLHFKTKEDLFRELAESVHRQM
                     GEAFCQVLGATAPGRDRVRGLIRAQLAFFETHRGLFLQVFQGNLPCLWRGPHRPMELV
                     KALINEMAKAIDEGARLGHFRRGRADEAAIGLFGLIRGFVFVHIAGDMAGSLLSRTEA
                     VWDLFSRGVCP"
     gene            complement(1267..2076)
                     /locus_tag="PK362_01380"
     CDS             complement(1267..2076)
                     /locus_tag="PK362_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73525.1"
                     /translation="MRLRPLLALSLLAACGKATFPAARLPEAVRELCLKEQKIDVRAQ
                     LVGKTLYIACDLEGLVTDDLDFRRDTLETFEGLMLSGTRASLSTDATVDFLVVQAKDP
                     RLKASITILRYIPDIKGLIYMRYSRSDFEDRMVLESDAESTEASTATWHDVDVAEFMA
                     RLVASRLQRRLSGNPLVSVMLQIRSIRGRVEGGDLILTLERGDKASTDSPVNAVLETA
                     VQEVASDVLRKYDPQSAEVQRVGLEDDRGTALWDRAATDLLSPSPKPLVSR"
     gene            complement(2048..3712)
                     /locus_tag="PK362_01385"
     CDS             complement(2048..3712)
                     /locus_tag="PK362_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC73526.1"
                     /translation="MSLFRRFKIFWMNNWVNITIIVSLILLLILSIWGLFALESFYRT
                     LTLATLPLNLLMAVMNALVFVYLYTHVLQGGFGKMGRKMQINADDFKVTFKDVIGLEN
                     AKKEAMEVVALLKDRARVKAIGGSIIKGLLMVGPPGTGKTLLAKAIATEAGIPFLSIA
                     GSEFVEVFVGVGASRVRKLFKKARQLAYAHGACIVFLDELDVIGRGRHFNSFGGGEET
                     NSTQNQLLVEMDGLGEKQENVIVIGATNAAEGVLDKALLRPGRFDRKIFVDLPNLQER
                     TELFRYYLRKIVHDPAIDIGRLARRCVQKSPADIMNICKESALIATRDKRSQITYRDI
                     SEAIERIDLGVAHPLSMSEQERQATAYHEAGHLLVLYNMHPTDDVFKASIIHRGGALG
                     VVYHTPREELHSSTKDQILADIKVSLGGYMGEKIKYGVTSNGVAADFANAMGKAHTMV
                     WRLGMGSNGFLGDYGVIPEHQLSDDIKSKLNQETYAILHQCAKDVEEFLRREWTLVDR
                     FAQELVRRNELEYDDIHAIFAEYGKAREQVPIQTVTLDAPPAAPGA"
     gene            complement(3750..4130)
                     /locus_tag="PK362_01390"
     CDS             complement(3750..4130)
                     /locus_tag="PK362_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC73527.1"
                     /translation="MPAKILIAEDDPNILGLLSVFLSGLGYEVALARDGQEALEMAAQ
                     VKPDLLIADVMMPRMNGFKLVNTLTSERHDIPMPKVIMLTSLADQENVQRGMSVGADV
                     YIPKPFNLEDVAARVKELLGAAGV"
     gene            4302..>5459
                     /locus_tag="PK362_01395"
     CDS             4302..>5459
                     /locus_tag="PK362_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC73528.1"
                     /translation="MAGPNPKERILVVDDEPHLLDLIVDLLTDDGYTVDGVASGTAAI
                     HAARQQEYHAALLDYNLGDMTGLALAKELRRLQEDLNVILMTAHASLDMAVKAIQADV
                     YDYLIKPIDSNHLKRSLANALEKNRLSVENKRLVQTLTKANDGLERASELKSKFLSIV
                     THDLRTPLASIRGYAQLLQFQRGLSPEQATQFLNIIIAESDHMGDLVSDLMDVVSIEA
                     GKLRIEKTPIVFNELLQQVASRMKPLAEQKKIVLATSLEAGDLPPLSLDRRRIDQVLT
                     NLIGNAFKHTPAGGQVSVSGRRDGETLRLEVADTGEGIPKEALTRVFDQFFQVEAHAS
                     KKEGLGLGLTIAREIIHAHGGEIGAESEGTGRGAKFWFTLPLTAAPNPPAPA"
BASE COUNT         1105 a         1679 c         1686 g          989 t
ORIGIN      
        1 ccggccgatg cggacccggc gggtcaaatc ggcgatccgc tgatcggtca attcccccaa
       61 ggtcgtgagc gccgaccggt ccgcgtcgta ggccagcacc gtgtaaaaca cctcggccac
      121 gtcgccgtaa agcaactggc gggcgcggac caagccccat cggcgggaat ccccgagggc
      181 ggattgagcc cgcattccgg caaactcccg aaacccggag aacaagactt ggcgtccgta
      241 aaaggccgac tcggaccgcc gcacccggga tccgccgccc gaggcgtcgg accgaccgcc
      301 ggcgtcttgc caggtttccg acgcgcgaaa gcccgcctgg ggcaataccc cggcccggac
      361 gagggcgtat tggttttcca cctggcgaat gtccgattcc gccagggcca aggtttcact
      421 tttggcggcg gcgcgccgga agcaatcccc cagggacagg gcggccgggg tttcctgggc
      481 ggagacggtc accgcggcca accagagcga taccacggtc caacgcctca cgggcaaacc
      541 ccgcgactga aaaggtccca cacggcttcg gtccgggaaa gaagcgagcc ggccatgtct
      601 ccggcgatgt gaacgaagac aaatccgcga atcaagccga aaaggccgat ggcggcctcg
      661 tcggcgcggc cccggcggaa atggcccagc cgggcccctt cgtcgatggc cttggccatt
      721 tcgttgatca gggctttcac cagctccatg ggccggtggg gcccccgcca gagacagggc
      781 aaattgccct ggaagacctg gaggaacagc ccccggtggg tttcaaaaaa agccaattgg
      841 gcgcgaatca atccccgaac gcggtcacgt ccgggggccg tggcccccag gacttggcaa
      901 aaagcctcgc ccatttggcg atgaaccgat tcggccagtt cccgaaaaag gtcttccttc
      961 gttttaaaat ggagatagag gctgcccttg gcgatgcccg accgtcgggc gatttcgtcc
     1021 atggtcaagg cgtgatagtc ccgggtttcc agggcgcgcc cggcggccgc cagaatggcg
     1081 tcgcgggttt cggcggccgg ccgtcgagcg tgcgggcggc ggcgaaggga cgtcgggggt
     1141 cgaacgaggg gaatgggaac gctcctgggc gatcgcgacc gggcggcaac cgcggaattg
     1201 actgaccggt cagtcaatat taggcgtttg aaggcgcctt gtcaaacgat cgacgggggg
     1261 aggcgatcag cgggaaacga ggggcttggg ggacggcgaa agaaggtccg tcgcggcgcg
     1321 gtcccagagg gcggtgccgc ggtcgtcctc caggcccacg cgttggactt cggcggactg
     1381 ggggtcgtat tttcgaagaa cgtcggaggc cacttcctgg acggcggttt ccaggacggc
     1441 gttgacgggg gaatccgtcg acgccttgtc gccccgctcc aaagttaata tcaagtcgcc
     1501 gccctccacc cggccgcgaa tggagcgaat ttgaagcatg acgctcacga gggggttgcc
     1561 gctcaaccga cgctggagtc gcgaggccac cagacgggcc ataaactcgg ccacatccac
     1621 gtcgtgccag gtcgccgtgg aagcctccgt ggattcggcg tcggactcca acaccatgcg
     1681 gtcctcaaaa tcggaccggg aatagcgcat gtaaatcagg cctttgatgt cgggaatgta
     1741 acggagaatg gtgatggagg ctttgagccg ggggtccttg gcctggacga ccaggaaatc
     1801 caccgtggcg tcggtggaca agctggcccg ggtgcccgac aacatcaggc cttcaaaggt
     1861 ttccagggtg tcccgtcgaa aatccagatc gtcggtgacc agcccttcca aatcgcaggc
     1921 gatgtaaagg gttttcccca ccaattgggc gcgaacgtcg attttttgct ctttgaggca
     1981 gagttcccgg accgcctcgg gaaggcgcgc ggcgggaaac gtcgccttcc cgcaagcggc
     2041 gaggaggcta agcgccagga gcggccggag gcgcatcgag cgtgaccgtt tggatgggca
     2101 cctgctcccg ggccttgccg tattcggcga agatggcgtg aatgtcatcg tattccaatt
     2161 cgttccgtct gaccaattcc tgggcaaaac ggtcgaccag cgtccattcc cggcgaagga
     2221 attcctccac gtctttggcg cattgatgga ggatggcgta ggtttcctga ttcaacttcg
     2281 acttgatgtc gtcggacaat tggtgttcgg gaatgacgcc gtagtcgccc aggaaaccgt
     2341 tggaccccat gcccagccgc cacaccatcg tgtgggcctt tcccatggcg ttggcgaaat
     2401 ccgccgccac cccgttggag gtcacgccgt acttaatctt ttcgcccatg tagcccccca
     2461 gggacacctt gatgtcggcc aggatttggt ctttcgtcga ggagtggagt tcctcccggg
     2521 gggtgtggta aacgacgccc agagcgccgc cgcggtggat gatggaggcc ttaaaaacgt
     2581 cgtcggtcgg gtgcatgttg tagagaacca acaaatggcc ggcttcgtga taggccgtcg
     2641 cttgccgttc ctgctcgctc atggacagcg gatgggccac gcccaggtcg atgcgctcga
     2701 tggcctcgga gatgtcccgg taagtgattt gggagcgttt gtcccgggtg gcgatcaagg
     2761 ccgactcctt gcagatgttc atgatgtccg cgggggactt ttgaacacag cggcgggcca
     2821 aacggccgat gtcgatggcg gggtcgtgaa caatctttcg aagatagtaa cggaacagtt
     2881 ccgtccgctc ctgaaggttc ggcagatcga cgaaaatttt ccggtcgaaa cggccggggc
     2941 gcaggagggc tttgtccaag acgccttccg ccgcgttggt ggcgccgatc acgatcacgt
     3001 tttcctgctt ttctcccagc ccgtccattt ccaccaacaa ttgattttgg gtgctgttgg
     3061 tttcttcgcc gccgccgaag gaattgaaat gccggccgcg accgatgacg tccaattcgt
     3121 ccaagaacac gatgcaggcc ccgtgggcgt aggccaattg gcgggccttt ttaaacaact
     3181 ttcgcacacg gctggcgccc accccgacga agacctccac aaactccgag ccggcaatgg
     3241 acagaaaagg gatccccgcc tcggtcgcga tggccttggc cagaagggtt tttcccgtcc
     3301 ccgggggtcc gaccatgagg aggcctttga tgatgcttcc cccgatggcc ttcacgcggg
     3361 cccggtcttt caggagagcc acgacctcca tggcttcttt tttggcgttt tcaagaccaa
     3421 tcacgtcctt gaaggtgacc ttgaaatcgt cggcgttgat ctgcatcttc cggcccattt
     3481 ttccaaatcc gccctggaga acgtgggtat aaaggtagac gaacaccaac gcgttcatga
     3541 ccgccatcag caagttgagg ggcagcgtgg ccagggtgag ggtgcggtag aaggattcga
     3601 gggcgaagag cccccaaatc gaaagaatca acagcagaat caacgacacg atgatcgtga
     3661 tgttgaccca gttgttcatc cagaaaatct tgaatcgacg gaaaagggac atgggaatcc
     3721 ttttggttcg actttgaacg tccgggggat tagaccccgg cggcgcccaa aagttccttc
     3781 acccgggcgg ccacgtcttc caggttgaac ggcttgggga tgtaaacgtc cgccccgacg
     3841 ctcatgcccc gttggacgtt ttcctggtcg gccagggagg tgagcatgat caccttgggc
     3901 atggggatgt cgtgccgttc gctggtgagc gtgttcacca atttaaaacc gttcatgcgg
     3961 ggcatcatga cgtccgcgat caacaaatcc ggcttgactt gggcggccat ttccagggct
     4021 tcctggccgt cccgcgccaa ggccacttcg taacccaacc cactgaggaa tacggacaac
     4081 agacccaaaa tgttcggatc gtcttcggca atcaggattt tggcgggcat ggtcacctca
     4141 aaaagtcgcg cgcgatgcga ttcagacgga aattcctcgt cgaaaacgtg gaatcaatca
     4201 attatagacc ccgatccctc ccccgtcaag gctttcccaa aatcgaccgt ccccccttgg
     4261 ggcccttgcc aagaaacgcc gggcgatcta ccctatggga catggccgga cccaacccca
     4321 aagaacggat cctggtggtg gacgacgaac cccacctcct ggacctgatc gtggaccttt
     4381 tgaccgacga cgggtacacc gtggacggcg tggcgagcgg gaccgccgcc atccacgcgg
     4441 cccgccagca ggagtaccac gcggccctgc tcgattacaa cctgggggac atgaccgggc
     4501 tggccttggc caaggaactg cggcggctcc aggaggattt aaacgtcatc ttgatgacgg
     4561 cccacgcctc cctggacatg gccgtcaaag ccatccaggc cgacgtctac gactacctca
     4621 tcaagcccat cgattccaac catctgaagc ggtccctggc caacgccctg gaaaaaaacc
     4681 gcctctccgt cgaaaacaaa cgcctggtgc agaccctcac caaggccaat gacgggctgg
     4741 aacgggccag cgagctcaaa tccaaatttc tttccatcgt gacccacgac ctgcggaccc
     4801 ccctcgcctc cattcggggc tacgcccaat tgcttcaatt ccagcggggg ctgtcgccgg
     4861 agcaggcgac ccagttcctg aacatcatca tcgccgaatc ggaccacatg ggggatttgg
     4921 tgtcggattt gatggacgtg gtgagcattg aggcgggcaa gctgagaatt gaaaaaacgc
     4981 ccatcgtgtt caacgaactc ctgcaacagg tggcctcgcg gatgaaaccc ctggccgagc
     5041 aaaagaaaat cgtcctggcg acgtccctgg aagccgggga tttgcccccc ctctccctgg
     5101 accggcggcg gatcgaccag gtgctgacca acctgatcgg caacgccttc aaacacacgc
     5161 cggccggcgg tcaggtgtcg gtttcgggcc gccgcgacgg ggaaaccctt cgcctcgaag
     5221 tggcggacac gggggagggg atacccaagg aggccttaac gcgggtcttc gaccaattct
     5281 tccaggtgga agcccacgcc tcgaagaaag aaggcctcgg gctgggcctc accatcgccc
     5341 gggaaatcat ccacgcccac ggcggcgaga tcggcgccga atccgaaggc accggccgcg
     5401 gcgccaaatt ttggttcacc ctgcccttga ccgccgcccc caacccgccg gccccggcc
//
LOCUS       DAOQLS010000042         5415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6817_length_5415_cov_4.312313, whole genome shotgun sequence.
ACCESSION   DAOQLS010000042 DAOQLS010000000
VERSION     DAOQLS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6817_length_5415_cov_4.312313"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PK362_01400"
     CDS             <1..913
                     /locus_tag="PK362_01400"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC73529.1"
                     /translation="LRINEKTPMESTPEIARDLRRLGELQIRRRAHADAEAALTRGIK
                     ITRIVHGDDHAETAEAWTLWGDLKADTGRFEEAAEGYGKAMFVWGKLTRKDAAGAAET
                     IKRQARLFELRGDGKKVEPTLLEALTHREKTVGPNDPGLAGDLLALGRHHNRHKLFRK
                     SLPLLGRALEIRQAARGPNHPDLAEILLASGDAQGLQGDVMQAEEFFHKALRLLEQNR
                     GAQCPELAGGLNDLAALYYDHSHYKEAEPLLRQAIDILQTVFGPAHPEVSTGLNNLMM
                     CLQSLGRSVETEGLKQQLIERARHLGR"
     gene            915..1859
                     /locus_tag="PK362_01405"
     CDS             915..1859
                     /locus_tag="PK362_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNC73530.1"
                     /translation="MEPLDPHLRRVFQPRQRRALLSFRRRRDLVFFIVCVVGLLVGVI
                     SLLSAIAVVKRRPAPPTVTTSPAPAVEPPAPPPLAVPPPTPEVPAAAPAPAAPPAAEP
                     PIAETPSRAIEPEPEPAVRPEKREKSKPPRAEKKSEPEKKTEPEKKKKSAKPADLPRL
                     ASPYLLLSNNRPMKIDADTTAEIIAETIVVGGQPALEIRYRMEEGRWVRVSFNTPSDL
                     HGARFVTLTFRGEGNTNAIEFGVVDADGTVASASWSKGTGTPTWSTAEIAVENLSRSG
                     GDGELDWSHVRSMYVRVAKGRSGEGGNGTALVRSVKIF"
     gene            2239..>5415
                     /locus_tag="PK362_01410"
     CDS             2239..>5415
                     /locus_tag="PK362_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNC73531.1"
                     /translation="MNFWDSALPAADSRSVAFPRVYFDPITHRYIATALGMTSNRIVI
                     RVSPPQFFAMVAVSETNDPTGPWKKYAFNLNVDAASPVRPVIGVNVTQTGFSHRWIAL
                     TLVATNLGVADGCHLYVFEKNDLINNSVTPATVTRFGKVANNPVAPMVPNRDTAQDDL
                     YFLESRFGDGGIFYSLGEVTDKGGPVVGAVASRNIVTPYLHISSSFTFHPQSGTTDKI
                     TASKSEIQSLVFDGSSLWSAQTVYAISPVRSAIQWHHWNPVNLADFDAGVLEDLTGAN
                     MYDFPSLSVNSVGDVLIGFSRFSGTTFPSAGYAFRAANDPAGTLRKPEIYKPGLAAYH
                     PLGAGANPWGYFSATALDPADGHSLWTLQNYAETPVFNGDTFDDAWGLQWARAIPPVA
                     APNMLPATALGVSSVQWNWSDNSYNESGFNLRRTSDNGAIVLSLAANTTFYRQTGLTP
                     NAPQQAYAQAFNPAISTNSGNSTVVYTLANPPTGTAVTFRSSNTVTLAWNANGNPAGT
                     EYRLQGATSVPLLALASFSTTTATSFSFNTLTADTTWFFRVRARNGGGVDTAFDATVS
                     TLTLPAPPAAPSAPDVVARSTGSLTWGWTDLAVNEAGFRVRRASDSVVLVGTLPANST
                     QWLQTGLTPNAPQRVFAESFNVTASSASGFSPTRYTLAAAPTASTVAVVAPQTARFSW
                     SANGNPAGTSYQISFAQSGFDPSFIPTTGTVRDFPGLTPNTTYEFQVSAQNTEGIFSA
                     PGALLTLWIPPLAPNAPSAVAAVAFSTDSVSWSWTDNADNEDGFRVRRSSDNANLSGD
                     LPVNSTGFVHAGLGPNAAARVRVEAFNVTAASASAPSATAYSLAVPPSSLGFLGVFQT
                     SAAFAWSAGANPAGTLFRAERSLNGVAYVEIATTTLTNVLASSLTPDTTYFFRVRAEN
                     TAGTTTAYSNVITTRTIPLAPAVPTGFAAIARTTGTLTWGWTDVSNEAGYRVRRSSDN
                     GFVSGDLPSNTVSFTQTSLGVNTPQQLYVEAFNAGGSAPSAPAAAHTLSNPPAAPVVA
                     TAGTSTVSLTWAANGNPAGTAYEVEQSLN"
BASE COUNT          965 a         1998 c         1512 g          940 t
ORIGIN      
        1 cctccgaatc aacgaaaaaa cccccatgga aagcaccccg gaaatcgccc gggatttgcg
       61 gcgcctgggt gaactgcaaa tccgccgacg ggcccacgcg gacgccgaag ccgccctgac
      121 ccggggcatc aagatcaccc gcatcgtgca cggcgacgac cacgccgaaa ccgccgaagc
      181 ctggacgttg tggggcgatc tcaaagccga cacgggtcgt ttcgaagagg ccgcggaggg
      241 ctatgggaaa gccatgttcg tttggggaaa actgacccgg aaggacgccg ccggggcggc
      301 cgaaacgatt aagcgccagg cgcgcttgtt cgaactgcgg ggcgacggca aaaaggtgga
      361 accgaccctt ctggaggccc tgacccaccg ggaaaaaacc gtcggtccca acgacccggg
      421 attggccggc gacttgctgg ccctcggccg ccaccacaat cggcacaaac tctttcggaa
      481 atcccttccc ctgctgggaa gggccttgga aattcgccag gcggcccggg ggccgaacca
      541 tccggatttg gccgaaatcc tgctggcctc cggcgatgcc cagggccttc aaggcgacgt
      601 catgcaagcc gaggaatttt tccacaaagc gctgcgattg ctggagcaaa accgcggcgc
      661 ccaatgcccc gaactggcgg gcggtttgaa cgacctggcg gccttatact atgatcacag
      721 ccattacaaa gaggcggagc ctctcctgcg ccaggccatc gacatccttc aaacggtttt
      781 cggcccggcc cacccggagg tttccacggg actcaacaat ttgatgatgt gcctccaatc
      841 cctcggccgg tccgtggaaa cggaaggcct caagcagcaa ttgatcgaaa gggcccgcca
      901 tctcgggcga taacatggaa cccctggacc cccaccttcg acgcgtgttc caaccccgcc
      961 agcggcgggc cttgctgtct ttccgccgaa ggcgggatct cgtatttttc atcgtgtgcg
     1021 tggtggggtt gctggtcggc gtgatcagcc tcctctcggc cattgcggtg gtcaaacgcc
     1081 gtccggcccc cccgaccgtg acgacgtccc cggccccggc cgtggaacct cccgcgccgc
     1141 cccccctcgc cgttccgccc ccgactccgg aggttcccgc ggcggcgccc gcgccggcgg
     1201 ccccgccggc ggcggagccc cccatcgcgg aaaccccgtc ccgcgcgatc gaacccgaac
     1261 cggaaccggc cgtccgcccg gagaaacggg agaagtccaa acccccccgc gccgaaaaga
     1321 aatccgagcc ggagaaaaaa accgaaccgg aaaaaaagaa aaagtcggcc aaacccgcgg
     1381 acttgcctcg actggcctcc ccctacctcc tgttgtccaa caaccggccc atgaagatcg
     1441 acgcggacac cacggcggaa attatcgctg aaaccattgt ggtcggcggc caacccgcgc
     1501 tggaaatccg ataccgaatg gaggaaggtc ggtgggtgcg cgtttccttc aacacccctt
     1561 cggacctgca cggggcgcgg ttcgtcaccc tgactttccg gggcgagggc aacaccaacg
     1621 ccattgagtt cggcgtcgtc gacgccgacg gcaccgtcgc cagcgcctcc tggtccaagg
     1681 gtaccggcac ccccacctgg tccaccgccg aaatcgccgt ggagaacctt tcccgttccg
     1741 gcggggacgg tgaattggat tggtcccacg tgcgcagcat gtatgtccgg gtcgccaagg
     1801 ggcgctcggg cgaaggcggg aacggcacgg ccctggtccg gtccgtcaaa atcttttaag
     1861 aagccccccg cgcgcggccg ggctgttccg gcgattgact tccccccctc tccgtgttaa
     1921 aatccccccg cttcccgacc gacaccccgg actctttctc attctctttt cctctttaaa
     1981 ggacaacgtc tgaaaaaccg ttttggcagc ctttggcgcg ccctccccct gggactgggc
     2041 ttcctcgtcg cgggccttcg ggcccaggcc gagcccgctc cggccgttca attccggggg
     2101 gacgcttacg accccgcctt cgcgttttcc ccacccagcg gacacggaac cgccggtccc
     2161 gaccacctcg ttttgtccga taacgggtac ttgatcgtcc aatcgaaatc gggcgttgcg
     2221 atcagctccc agactctctt gaatttttgg gattcggccc tgcccgccgc cgacagccga
     2281 tccgtcgctt tcccccgggt ttacttcgat cccatcaccc atcgctacat cgccacggcc
     2341 ctgggaatga cttcgaatcg gatcgtcatt cgggtctcgc cgccccaatt ttttgccatg
     2401 gtggccgtgt cggagaccaa cgatcccacc ggcccctgga aaaaatacgc cttcaacctg
     2461 aacgtcgacg ccgcctcgcc cgttcgcccc gtgatcggcg tcaacgtcac ccagacgggt
     2521 ttcagtcatc gctggatcgc cctgacgctc gtggccacca acttgggagt cgccgacggt
     2581 tgccacttgt acgtatttga aaaaaacgac ctgatcaaca attccgtcac gcccgccacc
     2641 gtgacccggt ttggcaaagt ggccaacaac cccgtcgcgc cgatggtccc caaccgcgac
     2701 acggcccaag acgaccttta ctttttggaa tcccgattcg gcgacggggg cattttttac
     2761 agcctgggag aggtgaccga caaaggggga ccggtcgtcg gcgccgtcgc ctcccgcaac
     2821 atcgtcaccc cgtaccttca tatttcctcg agtttcactt tccaccccca atcggggacc
     2881 accgacaaaa tcaccgcctc caaaagcgaa attcagagcc tcgtattcga cggatcctcc
     2941 ctgtggtccg cccagacggt ttacgccatt tccccggttc gttccgccat ccagtggcac
     3001 cactggaacc cggtcaattt ggccgatttt gacgccggcg ttttggaaga tttaaccggg
     3061 gcgaacatgt acgacttccc gtccctgtcc gttaattccg tcggggatgt cctgatcggg
     3121 ttctcccgtt tctcggggac gacgttcccc tcggcgggtt acgccttccg cgccgccaac
     3181 gacccggccg gcactcttcg aaaaccggaa atttacaaac ccggactggc cgcctaccat
     3241 cccctgggcg cgggggccaa cccttggggg tatttcagcg ccaccgccct ggacccggcc
     3301 gatggccaca gcctttggac ccttcagaac tacgccgaga cgccggtatt caacggggac
     3361 accttcgacg acgcctgggg ccttcaatgg gcccgggcca tcccccccgt tgcggccccc
     3421 aacatgctcc ccgcgaccgc cttgggggtt tcgagcgttc aatggaactg gtccgacaat
     3481 tcctacaacg aatccggatt caatctccgc cgtacctccg acaacggggc catcgttctt
     3541 tcgctggcgg ccaacaccac tttctaccgg caaaccggac tgacgcccaa cgccccccaa
     3601 caggcctacg cgcaggcgtt caacccggcg atcagcacga actccggcaa ttccaccgtt
     3661 gtctacacgc tggccaatcc gcccacgggc accgccgtaa ccttccgctc gtccaacacg
     3721 gtgacgctcg cctggaacgc caacggcaac cccgccggca ccgaataccg cctccagggc
     3781 gccacgagcg ttcccctgtt ggccctggcc tccttttcga ccaccaccgc gacctctttc
     3841 tcctttaata cgctgacggc cgacacgacc tggtttttcc gcgtccgggc ccggaacggc
     3901 ggcggggtgg acaccgcctt cgacgccacc gtatcgacgc tcacgctgcc cgcgcccccg
     3961 gccgcgccct ccgcgccgga cgtcgtcgcc cgaagcaccg gctccctcac ttgggggtgg
     4021 acggatctcg ccgtcaacga ggccgggttc cgggtccgcc gggcctccga ctccgtggtg
     4081 ctggtgggca cgctgccggc caacagcacc caatggcttc agacgggcct cacgcccaac
     4141 gcgccccagc gggttttcgc ggagtctttt aatgtcacgg cctccagcgc gtcggggttc
     4201 tcccccaccc gttacaccct ggccgccgcg cccacggcca gcaccgtcgc cgtcgtcgcg
     4261 ccccaaacgg cgcgattttc ctggtcggcc aatgggaacc cggcgggcac gtcctaccaa
     4321 atctccttcg cccaaagcgg gttcgacccg tcctttatcc caacgacggg aaccgtccgg
     4381 gacttccccg gcctgacccc gaacacgacc tacgaattcc aagtgagcgc ccagaacacg
     4441 gaaggaattt tttcggctcc cggcgccttg ctgaccctct ggattccgcc cctggccccc
     4501 aacgcgccgt cggcggtcgc ggccgtcgcc ttttccaccg acagcgtttc ctggtcctgg
     4561 acggacaacg ccgacaatga ggacggcttc cgtgttcgcc gctcctcgga caacgccaat
     4621 ctttcgggcg acttgccggt caattcaacg gggtttgtcc acgccgggct gggccccaac
     4681 gccgccgccc gggttcgggt ggaagccttt aacgtgaccg ccgccagcgc ctcggcgccc
     4741 tcggccacgg cctattccct ggccgttccc ccctcgtcct taggctttct cggggttttt
     4801 caaaccagcg ccgccttcgc ctggtccgcc ggcgccaacc cggcgggaac gttgttccgg
     4861 gccgaacgct ctctaaacgg cgtcgcctac gtggaaatcg ccaccacgac cttgaccaac
     4921 gttctggcct cgagcctcac gcccgacacg acctatttct tccgggtgcg agccgaaaac
     4981 accgcgggga caaccaccgc ctattccaac gtgatcacga cccgaacgat ccccctggcc
     5041 ccggcggtcc cgacgggttt cgccgcgatc gcccgcacga cgggcaccct gacctggggg
     5101 tggacggacg tttccaacga agcgggatac cgggtgcggc gctccagcga caacgggttc
     5161 gtttcgggcg acctgccgtc gaacaccgtt tccttcacgc aaacgagcct cggggtcaac
     5221 acgccacaac aactctacgt ggaagccttt aacgccgggg gctcggcccc ctcggctccg
     5281 gccgcggccc acacattgtc caatcccccc gccgcgccgg tggtcgccac cgcggggacg
     5341 tcgacggttt ccctgacctg ggccgccaac gggaacccgg cggggaccgc ctacgaggtg
     5401 gaacaatccc tgaac
//
LOCUS       DAOQLS010000043         5409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6829_length_5409_cov_5.476093, whole genome shotgun sequence.
ACCESSION   DAOQLS010000043 DAOQLS010000000
VERSION     DAOQLS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6829_length_5409_cov_5.476093"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="PK362_01415"
     CDS             complement(<1..1636)
                     /locus_tag="PK362_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNC73532.1"
                     /translation="MTPLSFQGLLRKPSKTRSLKGEILRSIAKIPPMPEIEEHFQKLI
                     DLIEIEREAEKEENKRELDRWPVETREQLGKTVSRLVIERQDVGVGGMPLLVLSRPPA
                     GEALSPFHAMDSGDIVRVTFLQGGFAKEGTLYDVEEYRVTVALNEELIDPPTGKCQID
                     LLGSDATYKRMRRALDIAQRATGRLGELRDVLMGLRSAQADPPKEIEFYNDGLNTFQK
                     DAVRSALAAKDVALVHGPPGTGKTTVLVEIIRQAALRGLTVLATAPSNIAVDNMLEKL
                     LDSDLRVVRLGHPARTLESLRHATLMAQMDEHPDQKPIALMDRDREQLVLQRYRRQNR
                     GTYDPVEDKEMKKEIKALWKEARNLEKALAKRIIQRAHVVLATHGGIGKNLSGERFDL
                     VCLDEASQSTEPLSWVPILLAQKIVLAGDPLQLPPTIYSKEAAAAGLKTTLMERLQPE
                     LPDNLQALLRVQYRMNETIMRFSSERFYEGKLIADGSVRDHDALGLPHVQPTDLTQGT
                     LVFVDTAGTGYAETWNELLDSRENEGEARLAALLWEE"
     gene            1635..2090
                     /locus_tag="PK362_01420"
     CDS             1635..2090
                     /locus_tag="PK362_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73533.1"
                     /translation="MGRTKGFTLIELMLVVAIIALLAAIAIPKFADLVIKAKEAGVKG
                     KLGTLRSAINLYYVDNEGTWPREVNLLGVPSVLVPKYLDEFPAISIPTVPSHALGSVH
                     TGSLAGGDPYVTDFLWFGAVQGWAYGWRTGTLVVNCTHTDSKGSTWSLW"
     gene            complement(2127..2249)
                     /locus_tag="PK362_01425"
     CDS             complement(2127..2249)
                     /locus_tag="PK362_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73534.1"
                     /translation="MKLSEVLQMKTLKPLWFPTMLVLTWCAFVLATTLWIRAGG"
     gene            complement(2437..3324)
                     /locus_tag="PK362_01430"
     CDS             complement(2437..3324)
                     /locus_tag="PK362_01430"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC73535.1"
                     /translation="MIVVTAATGHIGQHLVQELLSKKKKVRAVARDAQKLKKLADRGA
                     ETVTADLADPAAVARALQGASAVFALIPPSYGEPDFRGYQNKISVVLADAVKNAGVKH
                     VVNLSSLGAHLPDKTGPIKGLYDHERRLNQLDGVHVLHLRPTYFMENFYFGLGLIKGQ
                     GIHGSPLLPDVPFPMIATRDIASVAAHRLAALDFMGKSVQELLGPREVTMAEATRTIG
                     AAIGKPGLPYVQFPYADARAAMIGLGMSADVADQFNEMYKAFNEGLVRPTQARGAQTT
                     TPTSIEAFAREFAVAYSQS"
     gene            complement(3436..>5409)
                     /gene="rnr"
                     /locus_tag="PK362_01435"
     CDS             complement(3436..>5409)
                     /gene="rnr"
                     /locus_tag="PK362_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNC73536.1"
                     /translation="PKPANGPAPSHGAPGEIVEGVLQKKPRFGFVLSENPARPDLFIA
                     GPSLRLAMDGDRVRARVTRPGVRSEGEILEVLSRKRSTLAGVFRSTPKPCLVPEEGGE
                     PVRVADVGGLKPKDGDLCVLRVSQWPDGEHEAAGTLIEVIGRRNDPAADLAFLLRKHE
                     LPGEFPPEVERGAAAFGDEVPPSAYQGRKTFFDVPVFTIDGADAKDFDDAVSLERRPG
                     GGWRLGVHIADVAHYVTEGSALDQEAIRRGTSVYLSGAVIPMLPFPLSDNLCSLRPHV
                     ARLTLTCLMDLDPAGRVVHHDVFESVIRSARRFTYDEVELILQGQRVEVSDAVRDAVR
                     EMGELARTLRALRFGRGSLDFDFPEPVIIVDDKGWPIDACRKERGESHRLIEEFMLLA
                     NETVARHMAGGPFLYRIHERPDPAKMETLAKTLQAVGVAVPPTIASGKPAALRQVLES
                     VKGKPVEPMVHTLTLRSLKQAVYSPVNKGHFGLASEAYTHFTSPIRRYPDLLVHRMVK
                     ERIHGRFDAARQSAWRGRLPGQTEESSRLERRAVEAEREFLALKRAQLMTRRVGETFD
                     AVVTGVTAFGVFVQPTAVFVEGLVNVERLPRDRYQFDPLRLQLRGQRTNKSFRLGQAV
                     RVRLAGVNVEKRELDFALEDAPPKTGTFSRRPR"
BASE COUNT          999 a         1596 c         1721 g         1093 t
ORIGIN      
        1 attcctccca gagaagtgcc gccagccggg cttcgccttc gttttcccgg ctgtccaaaa
       61 gttcgttcca tgtttcggcg tatccggtgc cggccgtatc gacaaaaacc agggtgccct
      121 gggtcaaatc cgtgggttgc acgtggggca gacccagagc gtcgtgatcg cggaccgacc
      181 catcggcgat caatttcccc tcgtaaaaac gctccgaaga gaaccgcatg atcgtctcgt
      241 tcatgcggta ttggacccga agcaaggctt gcagattgtc cggcaattcg ggttgaagtc
      301 gctccatgag cgtcgttttg agtccggcgg cggccgcttc cttcgaatag atcgtcggag
      361 gcaattgaag gggatcgccc gcgaggacga ttttttgggc caggagaatc ggcacccagg
      421 acaggggttc ggtggattgg ctggcctcgt ccagacacac gagatcgaaa cgttcgccgg
      481 aaagattttt tccgatgccg ccgtgggtgg ccaacacgac gtgggcccgt tgaatgatgc
      541 gcttggcgag ggccttttcc aaatttcggg cctctttcca aagggctttg atctcttttt
      601 tcatctcttt gtcttcgacg gggtcgtagg tgccgcggtt ttgccgacgg taacgctgca
      661 ggaccaactg ctcccggtcg cggtccataa gggcgatggg tttttggtcc ggatgttcgt
      721 ccatttgggc cataagggtg gcgtggcgaa gactttccag cgtgcgggcc gggtggccca
      781 ggcggacgac ccgaaggtcg ctgtcgagca gtttttccag catgttgtcg acggcgatgt
      841 tcgagggcgc cgtcgccagc accgttaatc cccgaagggc ggcctgtcga atgatttcca
      901 ccaacacggt ggttttcccc gtgccggggg ggccgtggac gagcgccacg tccttggccg
      961 ccagcgccga acgaacggcg tccttttgaa acgtgttcag cccgtcgttg taaaattcga
     1021 tctctttcgg cggatcggcc tgggccgaac gcaggcccat caacacgtcg cgcaattccc
     1081 ccaaacgtcc cgtggcccgt tgggcgatgt cgagggcccg gcgcatgcgt ttgtaggtcg
     1141 cgtcggaccc gaggaggtcg atctggcatt tccccgtcgg cggatcgatc aattcctcgt
     1201 tgagggccac cgtgacgcgg tattcctcca cgtcatacag ggtgccttcc ttggcgaaac
     1261 caccctggag gaaagtgacc cggacaatgt cgccggaatc catggcgtga aaaggcgaca
     1321 gggcttcgcc cgccggggga cgcgaaagga ccagcaaggg cattccgccc acgcccacgt
     1381 cctggcgttc gatcaccagg cgggaaacgg ttttgcccaa ttgttcccgg gtttccacgg
     1441 gccaacggtc aagctcccgc ttgttttcct ctttttcggc ctctcgctct atttcaatga
     1501 ggtcaatcag tttctggaag tgctcttcga tctccggcat gggcgggatt ttagcaattg
     1561 agcgaaggat ttcgcctttt aaactcctgg tttttgacgg ttttcggagc aatccttgga
     1621 acgacagggg ggtcatgggt cggacaaaag gatttacgct catcgaattg atgctggtgg
     1681 tggcgatcat cgcgctgctg gccgccatcg ccattcccaa attcgccgat ctcgtcatca
     1741 aggccaagga agccggggtg aagggaaaac tcgggaccct tcgaagcgcc atcaacctct
     1801 attacgtgga caatgaagga acctggcctc gggaggtgaa ccttctgggg gtgccttcgg
     1861 tgttggtccc gaaatatctg gacgagtttc ccgccatttc catcccgacg gttccttccc
     1921 acgcgctcgg aagcgtccac accggatctc tcgcgggggg agatccttat gtgacggatt
     1981 ttttgtggtt tggcgccgtg cagggctggg cctacggttg gcggacggga acccttgtgg
     2041 tgaactgcac ccacaccgat tccaaaggtt caacctggag cctttggtaa atccgggggt
     2101 cgggggagcg gaattcggtt ggggaatcag ccgcccgcgc ggatccacag ggtggtcgcg
     2161 agcacaaagg cgcaccaggt gaggaccagc atggtgggaa accaaagggg ttttagcgtt
     2221 ttcatttgta gcacctcgct gagtttcatt tctgtcgccc ccttttgaca ctggtcggga
     2281 tcaaatgttc ccgtcctcac ccgatctact tgcaacgaat caaccaaaaa gatcccagca
     2341 aggtcattaa gagaaaaatg atctatttct agatataaaa aacgagggcc gtccccttcg
     2401 ggacggcccg gcgttcagcg gctattctcg acgaagttag ctctggctgt aggccaccgc
     2461 aaattccctg gcaaaagctt caatcgatgt gggggtcgtg gtttgggcac cccgggcctg
     2521 ggtggggcgg acaaggcctt cgttaaacgc cttgtacatt tcgttgaatt ggtcggccac
     2581 gtccgccgac atccccaggc cgatcatggc cgcacgggcg tccgcgtagg ggaattgaac
     2641 ataaggaaga ccgggttttc cgatggccgc gccgatggtc cgggtggctt ccgccatggt
     2701 cacttcccgg ggtcccaaaa gctcctgaac cgatttcccc atgaaatcca acgcggccaa
     2761 tcgatgggcg gccaccgacg cgatgtcccg ggtggcgatc atgggaaagg ggacgtccgg
     2821 aagcaagggg ctaccgtgaa ttccctggcc tttgatcaac ccgaggccga aataaaagtt
     2881 ttccatgaaa taggtcggcc gtaggtgcaa cacatggacg ccgtccaatt gattgagacg
     2941 ccgctcgtgg tcgtaaagac ctttgatggg tcccgtttta tccggcaggt gggcgccgag
     3001 gctggaaaga ttgaccacgt gtttcacgcc ggcgtttttc acggcgtcgg ccaagaccac
     3061 gctgatcttg ttttggtacc cccgaaaatc cggctccccg taggaaggcg gaatgagggc
     3121 aaacaccgcg ctggcgccct ggagggcccg ggccacggcg gccggatccg ccaaatcggc
     3181 cgtgaccgtt tcggcgcccc ggtcggcgag ttttttcagc ttttgggcgt cgcgggccac
     3241 ggcgcggact tttttcttct tggacaacaa ttcctgaacg agatgttggc cgatgtgacc
     3301 ggtggccgcg gtgacgacga tcatgactgc ctcccaatgg gacaataggt cgcgcggggg
     3361 gatcggcgaa gaccgtgaac gtacgcccgg cggaggccgg gtcgaacccc gaaaccgttc
     3421 agcggttcac cgactctaac gcgggcggcg ggaaaaagtt cccgtcttgg gcggggcgtc
     3481 ttccagggcg aaatccaatt cccgtttttc cacgttgacc ccggccagtc gcacccggac
     3541 ggcttgaccg agccggaagg atttgttggt ccgttggccc cgaagctgga gacgcaaagg
     3601 atcgaattgg taacgatccc ggggaaggcg ttccacgttg accaggcctt cgacgaacac
     3661 cgccgtgggt tgaacaaaaa caccgaaggc cgtgaccccg gtcacgacgg cgtcgaaggt
     3721 ttcccccacc cgtcgcgtca tcagctgggc gcgtttgagc gccaaaaatt cacgctcggc
     3781 ttctaccgcc cgccgctcca accgactgga ctcctcggtc tgccccggca gacgtccgcg
     3841 ccaggcgctt tgacgggcgg cgtcgaaccg gccgtgaatc cgctccttga ccatgcggtg
     3901 cacgagcaaa tccgggtacc gccgaatggg cgaggtgaag tgggtgtagg cctcggacgc
     3961 caggccgaaa tgtcctttgt taacgggcga gtacaccgcc tgtttcaagc tgcgaagggt
     4021 caaggtgtgg accatgggct ccaccggttt tcccttgacg ctttccagca cttggcggag
     4081 ggcggccggt ttcccggagg cgatggtcgg cgggaccgcg acgcccacgg cctgaagggt
     4141 tttggcgagg gtttccattt tcgccggatc cgggcgttcg tggatccggt acaggaaggg
     4201 gccgccggcc atgtggcggg cgacggtttc gttggccaac agcatgaatt cttcgatcag
     4261 tcggtgggat tcgccccgtt ccttgcggca ggcgtcgatg ggccaccctt tgtcgtcgac
     4321 gatgatcacg ggttcgggaa aatcgaagtc caaggagccc cggccgaacc ggagggcccg
     4381 aagggtccgg gccagttcgc ccatttcccg gacggcgtcc cggacggcgt ccgagacctc
     4441 cacccgttgc ccctgaagaa tgagctccac ttcgtcgtaa gtaaatcggc gggcgcttcg
     4501 aatgacgctt tcaaacacat cgtggtggac gacccgcccg gccgggtcga ggtccatcag
     4561 gcaggtcaac gtcaaccggg ccacgtgggg acggagactg cacaggttgt cggaaagcgg
     4621 gaagggcaac atgggaatga ccgcgcccga caaatacacg ctcgttcccc ggcgaatcgc
     4681 ttcctggtcc agggcgctgc cttcggtgac gtagtgggcc acgtcggcga tgtggacgcc
     4741 gagacgccag ccgcccccgg gtctgcgctc cagggacacg gcgtcgtcga aatccttggc
     4801 gtcggcgccg tcgatggtga agaccggcac gtcaaaaaac gtttttcgtc cctggtaggc
     4861 cgacggcgga acttcatcgc cgaacgccgc ggccccgcgc tccacctcgg gtggaaattc
     4921 gccgggcaat tcgtgctttc gaagcagaaa ggccaaatcg gcggcggggt cgttccgccg
     4981 gccgatcact tcgatcaacg tgcccgccgc ttcgtgttcg ccgtcgggcc attggctcac
     5041 gcggagaacg cacaagtcgc cgtccttggg cttcaaaccg ccgacgtcgg ccacgcggac
     5101 gggttcgccg ccttcttcgg gcaccaggca gggtttgggc gtggagcgaa acactccggc
     5161 caaggtggag cgtttgcggg agaggacttc caaaatttcg ccttccgaac ggaccccggg
     5221 ccgggtcacc cgcgcgcgaa cgcggtcccc gtccatggcc agacgcaaac tgggaccggc
     5281 gatgaacaaa tccggccgcg cggggttttc ggaaagcaca aagccgaagc ggggtttttt
     5341 ttgaaggacg ccttcgacga tttcgcccgg cgcgccgtgg gacggcgcgg ggccgttcgc
     5401 gggcttggg
//
LOCUS       DAOQLS010000044         5333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_6998_length_5333_cov_3.955665, whole genome shotgun sequence.
ACCESSION   DAOQLS010000044 DAOQLS010000000
VERSION     DAOQLS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6998_length_5333_cov_3.955665"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5333)
                     /locus_tag="PK362_01440"
     CDS             complement(<1..>5333)
                     /locus_tag="PK362_01440"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC73537.1"
                     /translation="AMMSRRLGLPVRRDAVFAGEVDLRGRVLDVPQVRERAIAAREAG
                     AAFLFLPSAAEPNLFQEAFKTAPYLMGPVLNLATGRLWLKERSLPARSRAAEVAAIAH
                     WNEAIRRVAETPPGALRVQKGDRPGETMVEGSRNDLVALLASLPRLSGQLTYCLLEDP
                     TEAVRLGLLGSGGFPVFPPPTTKSESEDAEPEAESARPRAGTISSPPSSTDEAFPPTV
                     AAPRGNPAEWLLGSAHEPLAVGPLLDDFEENGELLADALDYTLEHNTNENREKLRELL
                     LALVYTHPVAMSDVFNTIVSEPENSDHRDFLFQEMPAEIVGVMLANHRRGLLHYVQSG
                     AQKKARQFEEYENLLLNAVHNPPADEASRAALVKTLGAFVDAARPEVESASNAGNREG
                     LLEPFQRLEKAVPEIQKTPLDKLADAFGSLADPILALTTFLKARSYDFAFPPMSANQR
                     EVEKIIDAHLNKLIEESGAGSFAMLTREDEKAGRVAAIWRSYLASLSVENEIQRGRLA
                     IHNAEAITHLSPEFQATFEEATGVVRDLLDRQVFRSVNVVCPSDNTKAVFPGLLAERL
                     KNDHRPRRVVTVEVNQFPVDPRDSLREILSLMQAAYDAGDVVLVIDLDEFRSRFGDIR
                     ARSLSRVLGRWASHRNPLPLVFLSNEETNADFTENLPVFFRWIPQHRLRIANAKVLLD
                     YELGALEKTGVSVEPEARLWLQIKLAREEVDFALAAMLLRDGAAEAHRQHRPLTVDDL
                     EATWVTMQSGELAARVRMLPDSARRRGMALLAQMNNAQEGSHEYGNIRDVLENLVRFP
                     WLKRTRSPLPALAEVDPRLRADEFRRAGRQILQNARRHLDESHYGMNEAKEEIEDYLA
                     EQIQRERHGKRGTGKVLCLVGPAGVGKSTLGESIAKALGREFGRVPLGGTRDPAEIRG
                     HSPTYQGAMPGNIVQTLQKLQVKNPVILLDEIEKMDSGIQGNPADVLLQALDEEQNDH
                     FTDHNIGDVDLSEVLFIATANEEDKIPEALKDRMEIVRLPAYDRAEKIAIGTDKLLPR
                     VMRDLGVSPEQVQTPRPQELMTHLVDGYTQEPGVRELSKQLTRLVKRALTDVYLAGGR
                     PVVLTPEAVRGYMGLPRRGATARASSEVGEAVGLVISPRGGGTINIQADARPANNPEA
                     PIEIVNFGQLQADMLFSVETALSYLRKNAVRLGLPPAAMKGQRLLINISPGDVKKDGP
                     SAGIGFVATIFSSLTGQPLRQDFAMTGQVDDEGKVYPIGGLKEKILAALEDRASTVIL
                     PVGNKEELIKTIFVQSPDLKGVIEENGLQRITVEQKKPAGNQTGPTAEDSTWEALNTL
                     LTTKAQRFGLVAESDRLGALTLAGTPDQMRSFLSDSDVMKNVSYPVTYLIVSHVDEAL
                     KALRAAGNDESSGGTKPTLPLTGPALGGVLTAFLAGLAYSPESSAAPLVQSLATAGTL
                     SFASLAPVLLLTGWMALKWIARRWNTTVLSRNTLHRMTISLGGALADLAGKGLVGLFE
                     FGGFLRRGPGEAPSPLGGGLFADLRDIQTMENQYPLLAAALSTAARHHWFSRGPLSLT
                     TSVSTRALAGFTERMLAAGDTRRIRYPYAARAAGAGFRPTPRTKNATDEGLGSPQRLG
                     VLLHGVSEGLPSDLIDWRLKNMEGLDRGEWPNADSLRTLLDFLRGDSWKSPSYEGLEE
                     ERREFVRAQIVEIRRSLYARLALEAARADPLEGRKVWETGKTAESDARVTERSSGDGW
                     RGAADALALRPAVVHSREAIVLDAELLKAAAAEDVARLVAAAFNNSASKTMA"
BASE COUNT         1044 a         1654 c         1578 g         1057 t
ORIGIN      
        1 gccatcgttt tggacgccga attgttgaag gccgccgcca ccaatcgcgc cacgtcttcc
       61 gcggcggcgg ccttcaacaa ttcggcgtcc aaaacgatgg cctcgcggga atgaacgacg
      121 gcggggcgaa gggccaaggc gtcggccgcg ccgcgccaac cgtcgccgga ggaacgctcc
      181 gtgacgcggg catcgctttc cgctgttttc ccggtttccc acacctttcg gccttccaag
      241 gggtccgccc gcgcggcctc caaggccaat cgcgcataca acgaccgccg gatttcaacg
      301 atttgagccc ggacgaattc ccttcgctcc tcttccaaac cttcataaga gggggatttc
      361 caggaatcgc cgcggaggaa atccaggaga gtgcggaggg agtcagcgtt cggccattcg
      421 ccgcgatcca gaccctccat attttttagc ctccaatcga tgaggtccga aggcaagccc
      481 tcggagacgc cgtgaagaag aacgcccaat cgttgggggg aacccagccc ttcgtccgtt
      541 gcatttttcg ttcggggggt cggccgaaaa ccggccccgg cggcccgtgc cgcgtatgga
      601 taccggatcc gtctcgtatc gcccgcggcc agcattcttt cggtaaaacc ggccaacgct
      661 cgcgttgaaa cgctcgttgt cagcgaaagg gggccccggg aaaaccaatg atgccgggcc
      721 gccgttgata aagcggccgc caaaagggga tattggtttt ccatcgtttg aatgtcgcgg
      781 agatccgcaa aaagtccacc gcccaacggc gacggggcct ccccagggcc cctccgcagg
      841 aatccgccaa attcaaagag ccctaccaaa ccttttcccg ccaggtcggc caaagcgccg
      901 ccgagcgaga ttgtcattcg atgcaaagtg tttcgggaaa gaaccgtcgt attccatcgc
      961 cgagcaatcc attttaaagc catccatccg gttaacagca gaacgggagc aagggaagcg
     1021 aaggacaacg tcccggcggt ggccaacgat tgaaccaggg gcgcggccga ggattccggc
     1081 gaataggcca atccggccaa aaaggcggtg aggacacccc ccaaggccgg accggtcaag
     1141 ggaagcgtgg gtttcgtccc gccggacgat tcgtcgttgc cggccgcccg gagagccttc
     1201 aacgcttcgt ccacgtggga aacgatcaaa taggtcacgg gataggacac gttcttcatc
     1261 acgtccgaat ccgagagaaa ggatcgcatt tgatcgggcg tgcccgccaa ggtgagggcc
     1321 cccagccgat ccgattcggc gaccaaacca aaacgctggg ccttggtggt taaaagcgta
     1381 ttcagcgcct cccaggtgga atcctcggcc gtgggcccgg tttgattgcc ggcgggtttc
     1441 ttttgctcga cggtaatgcg ttgaagaccg ttttcttcaa tgaccccttt caaatccggg
     1501 gattgaacaa atatcgtctt aatcaactcc tccttgttcc cgacgggtag gatgaccgtt
     1561 gaagcgcggt cttccagggc ggcgagtatt ttttccttca aaccgccgat gggataaact
     1621 tttccttcgt cgtccacttg ccccgtcatg gcaaagtctt gccgcaaagg ctggcccgtc
     1681 aacgaggaaa atatggtggc cacaaaaccg atccccgcgg agggcccgtc tttcttcacg
     1741 tccccgggcg atatattgat caacaaccgt tgccccttca tggccgcggg cggcaggccc
     1801 aaccgaaccg cgtttttgcg aagataggac agggcggttt ccacgctgaa caacatgtcc
     1861 gcctgaagct ggccaaaatt aacgatttcg atgggggctt cggggttgtt cgccggtcgg
     1921 gcgtccgctt ggatgttgat ggtgccgccc ccgcgggggg aaatcaccag cccgacggct
     1981 tcgccgactt cggaagaagc tcgggccgtc gccccgcggc gcggcaaccc catataaccc
     2041 cgcaccgcct cgggggtcag gacgacggga cggccgccgg ccaagtagac gtcggtgagg
     2101 gcgcgcttca ccaaccgcgt caattgtttg ctcaattccc gaacgcccgg ttcctgcgtg
     2161 taaccgtcca ccaaatgcgt catcaattct tgcggccgcg gtgtttggac ttgttccggg
     2221 gaaaccccca ggtcccgcat gacccgggga agcagtttat ccgttccaat ggcgattttc
     2281 tcggcccgat cgtacgccgg caaccggacg atttccatcc ggtctttcag cgcttcgggg
     2341 atcttgtctt cttcgttggc ggtcgcgatg aacaaaactt ccgaaagatc gacgtcgccg
     2401 atgttgtggt cggtgaaatg atcgttttgc tcctcgtcga gcgcttgaag caggacgtcg
     2461 gccgggtttc cttgaatgcc ggaatccatt ttttcaattt cgtccaggag gattacgggg
     2521 tttttgacct gcagtttttg cagcgtctga acgatgttgc ccggcatggc gccttggtag
     2581 gtcggtgaat gcccgcgtat ttccgcggga tcgcgcgttc ctcccagggg cacccggccg
     2641 aactcccttc ccaaggcttt ggcgatggac tccccgagcg tggatttgcc caccccggcc
     2701 ggccccacca ggcaaaggac tttgccggtg ccccgcttgc cgtgtcgttc ccgttgaatc
     2761 tgttcggcga gatagtcttc gatttcctct ttggcctcgt tcatgccgta atggctttcg
     2821 tccaaatgac ggcgcgcgtt ctgaaggatt tggcgcccgg cccggcgaaa ttcgtcggcg
     2881 cgcaggcgcg ggtccacttc ggcgagagcg ggcagggggc tgcgcgttct tttaagccag
     2941 gggaaccgca ccaggttttc gagaacgtcc cgaatattcc cgtattcgtg ggaaccctcc
     3001 tgggcgttgt tcatctgggc caaaagggcc attccccgcc gtcgagcgct gtccggcagc
     3061 atgcgcacgc gcgccgccaa ctccccggat tgcatcgtga cccaggtggc ttccaaatcg
     3121 tccaccgtga ggggccggtg ctgccgatgg gcctcggccg cgccgtcccg caaaagcatg
     3181 gccgccaacg cgaaatcgac ttcctcccgg gccaacttga tctgcaacca aaggcgcgcc
     3241 tccggttcaa ccgaaacacc ggttttttcc agggcgccca actcgtaatc caatagaacc
     3301 ttggcgttgg cgatccgaag ccgatgttgc gggatccagc ggaagaagac cgggagattt
     3361 tcggtgaaat ccgcgttggt ctcctcgttc gacagaaaaa cgagcggaag cggatttcga
     3421 tgactggccc atcgtcccag aacccgcgaa agagatcgcg ctcgaatgtc cccgaaccgg
     3481 gaacgaaact cgtccaaatc aatcaccagc accacgtcgc cggcgtcgta agccgcctgc
     3541 atcaaggaga ggatttcgcg cagggaatcc cggggatcca cggggaattg gttgacttcg
     3601 acggtcacca cccgccgggg ccgatgatcg tttttcaacc gctccgcgag aaggccggga
     3661 aaaacggctt tcgtgttgtc cgacgggcaa acgacgttga ccgatcgaaa cacttgacga
     3721 tccagcaaat ctcgaaccac gccggtcgct tcttcaaaag tggcttggaa ttcgggcgac
     3781 aggtgggtga tcgcttcggc gttgtggatg gccaaacgcc cccgctgtat ttcgttttcc
     3841 accgacagcg aggccaaata cgaccgccaa atcgcggcca cccgcccggc tttttcgtct
     3901 tcgcgggtca acatggcaaa agacccggcc ccggactctt caatcaattt attcaaatga
     3961 gcgtcaatga tcttttcgac ttcccgctgg ttggcgctca tggggggaaa ggcgaaatcg
     4021 taggagcggg ctttcaaaaa cgtggtgaga gccagaatcg gatcggccaa cgacccaaag
     4081 gcgtcggcca atttatcgag cggggtcttt tgaatttccg gaacggcttt ttccaatcgt
     4141 tggaacggtt ccaacaaccc ttcccggttg ccggcgttgc tcgccgattc cacttccggg
     4201 cgcgcggcgt cgacaaaggc cccgagggtt ttgacgagcg cggcgcggga cgcctcgtcc
     4261 gcgggcggat tgtgaacggc gttcaatagc agattctcgt attcctcgaa ctgccgggct
     4321 ttcttttgcg ctcccgattg gacgtagtga agcaatccgc gtcgatgatt ggccagcatc
     4381 acgcccacga tttcggcggg catttcctgg aaaaggaagt cgcggtggtc gctgttttcg
     4441 ggttcggaaa cgatcgtgtt gaaaacgtcc gacatggcca cggggtgggt gtaaaccagc
     4501 gccaggagca attcccgtaa tttttcgcgg ttttcgttgg tgttgtgctc cagggtgtag
     4561 tccaacgcgt cggccagcaa ttctccgttt tcttcaaaat cgtccaggag cgggcccacg
     4621 gccaaaggct catgggcgga tcccaaaagc cattccgccg gattcccccg cggggcggcg
     4681 accgttggag gaaacgcctc gtccgtggag gacggcgggg atgaaatcgt ccccgcccgg
     4741 gggcgggccg actcggcttc cggttccgcg tcctcgcttt cggatttggt cgtcggtgga
     4801 gggaaaacgg gaaacccgcc gcttcccaac aagcccaggc gaacggcttc ggtgggatcc
     4861 tccaggaggc aataggtcag ttgtcccgat aatctcggca acgacgccag gagcgccacc
     4921 aaatcgtttc gcgaaccctc caccatggtt tctccgggcc gatcgccttt ttgaacccga
     4981 agagcgccgg ggggcgtttc ggccactcgc cgaatggcct cgttccaatg ggcgatggcg
     5041 gcgacctccg cggcccggga cctcgccgga agggaccgtt ccttcaacca aagtcgaccc
     5101 gtcgccagat tcaagaccgg gcccatcaag tacggcgccg ttttaaaggc ttcctggaac
     5161 agattcggtt cggccgccga aggcaaaaac aagaacgccg cccccgcctc gcgcgccgcg
     5221 atcgcccttt cacgcacctg cgggacgtcc aacacccgcc cgcggagatc cacttcgccc
     5281 gcaaaaacgg cgtcccgccg aaccgggagt cccaaccgac gggacatcat ggc
//
LOCUS       DAOQLS010000045         5318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7028_length_5318_cov_5.160175, whole genome shotgun sequence.
ACCESSION   DAOQLS010000045 DAOQLS010000000
VERSION     DAOQLS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_5318_cov_5.160175"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2332
                     /gene="bamA"
                     /locus_tag="PK362_01445"
     CDS             <1..2332
                     /gene="bamA"
                     /locus_tag="PK362_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNC73538.1"
                     /translation="QGFNAMNRTHRWLARIATALILGFAVPLRAETDPVIKDIRVEGN
                     RFLRPRTVLSKVKAKAGDTVTDPAFRADIDRLFETGQYDDVEVSLEDVPGEKTAQGAP
                     KVRVVFKIKERPVVRRIDFKGNRRLSDSKFREELSSKTDEPYDRFKAGQDVQKIIAKY
                     REEGYAEAQAESYVSLNPRTNKVILTFFITEGNRVLVDAVNVEGNRFMTARKVRGRLK
                     KTRRKKVFKEDTYKEDIDHLIEAYRDQGFLEVQVNASSRSFSEDHTRVTLTLGVREGR
                     RYTVGGVAFAGATLYKDAELSKAVALRSGKLYEQSKMNESLRSLQDLYADKGYLRAEI
                     DPQTLTHPVDETRGTVDVTFAIVESSVVYVDRVYVDGNTYTKEKVVRREVLLKPGDVF
                     SAGKMRRSVERIYNLGFLDDVQVDIQQPRSPSLADVVFSVKEGKPGILSAGAGFSSLD
                     GVVGTLQVQHVNLFGLGQRLNLMWEFGRKKQNYEIGWTDPWFLNKPVSFGVDLFDTVR
                     TLPFQNDSFAYKKGNRGFGLRLGPRLSDELSLIENYNYERVRVFDIEPKFIGNADPAN
                     NILPSDDIKSAFTTGAVWDTRDNVFDASRGGRHSATVEVAGGPFGGDLNFYKPELSVA
                     RYFPTIWKFVLTLSARGAFVKEFPPSKDLPFSELFRVGGVDTVRGYDIGEVGVDGGHT
                     YMVYNAEYKFPIVQENHRTILQGAFFADVGGSWAGNRDVNLGIGSRLNQMRSGVGFGV
                     RFKTPVFPIRLDWGYGLNHLPGQPLSQFYFTIGNIF"
     gene            2420..3082
                     /locus_tag="PK362_01450"
     CDS             2420..3082
                     /locus_tag="PK362_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNC73539.1"
                     /translation="MSPAHSQWNRTFVAALAVAGCTVAAGSSLGALEIPLKRGANQAT
                     RVGFVDMDKIFREYPETQKARAEYYKALEKRRSELAAREKELAALDGPAPASAPATPP
                     VISTGLFALPPDLMSPTASTGPAAIPAPDPEEREAVRREKQAEIAEARAAAARELKEL
                     EEKKSVQILGSLYKTLVQLAQEKGVDLVVDKSAILYGQNAIDLTEPLARRLRGLPDDG
                     KP"
     gene            3093..4115
                     /gene="lpxD"
                     /locus_tag="PK362_01455"
     CDS             3093..4115
                     /gene="lpxD"
                     /locus_tag="PK362_01455"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNC73540.1"
                     /translation="MKLTVTQIAELTGGAVRGDGATVIEGAAGLADATPRDITFLGND
                     KYQSQLETTRAGAVLVAPGVDTGSRPAVVVKNPPYGWARVLEVLEKERTRRPAGIHPT
                     AQVAPTARVGKNVTIGAFTVVEDGAVIGDNSVLYAHVYVGFDVRIGRDCLIYPRATLR
                     ERVALGDRVVLQPGAVIGCDGFGFTVHEGRHYKIPQVGTVEIGDDVEIQANSTIDRAA
                     VGVTKIGRGTKIDNLVQIAHNTEIGEHCLIVALTGIAGSVKVGRYVTLAAQVGVAGHL
                     SIADGVTVAARGGVTQSLKTPREVLWGLPAQPIRDELKCQAALRRLPAVLEEWRQFKK
                     KGSPSA"
     gene            4112..5017
                     /gene="lpxC"
                     /locus_tag="PK362_01460"
     CDS             4112..5017
                     /gene="lpxC"
                     /locus_tag="PK362_01460"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNC73541.1"
                     /translation="MKLFSTGERQRTIAREAVYKGVGLHTGNKCHLIFKPAPAGQGVV
                     FVRTDLPGKPRLPAHHSIVSSVVRGTTLSLDGEKNHEARVHTVEHVLSALYGLGIDNA
                     VIEVNANEPPVADGSARPFWDALEAAGVTELDEPRRFFQPDPLEYTAGETRYEVEPAD
                     ELIIETNIHFKHPLIGEQRREFTVTRESYLNEISRARTFCFDFEVEALKRQGLARGGS
                     LDNAVVVGMDRIHNKEKSLRYPDEFVRHKTLDLIGDLFLLGAPLKARIKAHRVGHGHN
                     VNLVKKLAAQMTGEAPSQTAVTSNY"
     gene            5043..>5318
                     /locus_tag="PK362_01465"
     CDS             5043..>5318
                     /locus_tag="PK362_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HNC73542.1"
                     /translation="MDNVAPRTLTTVEIMGAIPHRYPILMIDTATITEPEKKITGFKS
                     VSANEPFFQGHFPGRPIMPGVLIVEAMAQSACVLFLSKPELKDKLAFF"
BASE COUNT         1063 a         1670 c         1630 g          955 t
ORIGIN      
        1 ccaaggattc aacgccatga accgaaccca tcgctggttg gcccggatcg cgacggccct
       61 gattctcgga ttcgccgtcc ctttgcgggc cgaaaccgat cccgtgatca aggacattcg
      121 ggtcgagggg aatcggtttc tccgtccccg caccgttctt tccaaagtga aggccaaagc
      181 gggggacacg gtgacggatc ccgcttttcg ggccgacatc gatcgattgt ttgaaaccgg
      241 ccagtacgac gacgtcgaag tctccctgga ggacgtgccc ggcgaaaaaa cggcccaggg
      301 cgcgcccaaa gtgcgggtgg tcttcaaaat caaggagcgc ccggtggttc gccgcatcga
      361 tttcaaggga aaccgccggc tttccgatag taaattccgc gaggagctgt cgtccaaaac
      421 cgacgagccc tacgatcgct ttaaagccgg tcaggacgtt caaaaaatca tcgccaaata
      481 ccgggaggaa gggtacgccg aggcccaggc ggagtcctac gtttccttaa acccccgaac
      541 caacaaagtc attttaacct tcttcattac cgaggggaac cgcgtgttgg tggacgccgt
      601 caacgtcgag ggcaaccgct ttatgaccgc ccggaaggtc cggggccggc tcaagaaaac
      661 acgccgcaaa aaagttttca aggaagacac gtacaaagag gacatcgacc acctcatcga
      721 ggcttaccgg gaccaggggt tcctggaggt tcaggtgaac gcctcctcac gctccttttc
      781 ggaggaccac acccgggtga cgttgaccct gggcgtccgg gaagggcgcc gttacacggt
      841 gggcggcgtc gctttcgcgg gcgcgacgct ctacaaggac gccgagctgt ccaaggccgt
      901 cgcccttcgg tcgggaaaat tgtacgagca atccaagatg aacgaatccc tgcggtccct
      961 gcaggatttg tacgcggaca aggggtatct gcgggcggaa atcgatcccc aaaccctcac
     1021 ccaccccgtg gacgaaacgc gggggacggt cgatgtgacc ttcgccatcg tcgaaagctc
     1081 ggtcgtctac gtggaccggg tgtacgtgga cggcaacacc tacaccaagg agaaggtggt
     1141 gcgccgggaa gtgctgctca aaccgggcga cgttttttcc gcgggcaaga tgcggcggtc
     1201 ggtggagcgc atttacaatt tgggcttttt ggacgacgtg caggtggaca ttcagcagcc
     1261 ccgctccccg tccctggccg acgtggtctt ctccgttaag gagggcaagc ccggcatcct
     1321 gtccgccggc gcgggttttt ccagcctgga cggcgtggtg ggaacgctcc aggtccagca
     1381 cgtcaatttg ttcgggttgg gccagcggct gaatttgatg tgggaattcg ggcgcaaaaa
     1441 acagaattac gaaatcggtt ggacggaccc ctggtttctc aacaagcccg tgagtttcgg
     1501 cgtcgacctc ttcgacacgg tccggaccct ccctttccaa aacgattcct tcgcctacaa
     1561 gaaggggaac cgcggttttg ggcttcgtct cggtccccgc ctgtcggacg agctgtcgct
     1621 gatcgaaaat tacaactacg agcgcgtccg ggtcttcgac atcgaaccca aattcatcgg
     1681 caacgccgac ccggcgaaca acatcctccc ctcggacgac atcaaatccg cgttcaccac
     1741 cggcgcggtg tgggacaccc gggacaacgt gttcgacgct tcgcggggcg gccgccacag
     1801 cgcgacggtc gaggtggcgg gagggccttt cgggggcgat ttgaatttct acaaaccgga
     1861 actgtccgtg gcgcgctatt tcccgacgat ttggaaattc gtgctgacgc tctcggcccg
     1921 gggcgccttc gtcaaggaat tcccgcccag caaagacctc cccttctcgg aattgttccg
     1981 agtgggcggc gtcgacaccg tccggggata cgacatcggg gaggtcggcg tggacggcgg
     2041 gcacacctac atggtgtaca acgccgaata caagtttccc atcgtgcagg aaaaccaccg
     2101 caccatcctt caaggcgctt ttttcgccga cgtgggcggc agctgggcgg gcaaccggga
     2161 cgtcaatctc gggatcggca gccgcctcaa tcaaatgcgg tccggcgtgg gattcggcgt
     2221 ccgattcaaa accccggtct tccccattcg cctcgattgg ggttacggat tgaaccattt
     2281 gcccggacag cccttgagcc agttctattt caccatcggg aacatcttct aagcgcgttc
     2341 ctcggcggtg gacagccccc acccaatagt gtaaaatcga cgttttaaag ccgcttcgcc
     2401 ccgtacgggg gaggattttt tgtcgcccgc ccattcccag tggaatcgaa cctttgttgc
     2461 cgccctcgcg gtcgccggat gtacggtggc cgccggatcg tcgttggggg cgctggaaat
     2521 tcctctgaag cggggggcca accaggcgac ccgtgttggg ttcgtcgaca tggacaagat
     2581 ttttcgggag tacccggaga cccaaaaggc ccgggccgaa tattacaagg cgttggaaaa
     2641 gcgacggtcc gagttggccg cccgggaaaa agaattggcg gccctggacg ggcccgcgcc
     2701 cgcgagcgcg cccgccacgc cgccggtgat ttcgaccggg ctgttcgccc tgccgccgga
     2761 tttgatgtcc ccgaccgcct ccaccgggcc ggccgcgatt ccggcccccg atcccgagga
     2821 gcgggaagcg gtccggcggg aaaaacaggc cgaaatcgcc gaagcgcggg ccgccgcggc
     2881 ccgggaattg aaggagctcg aggaaaagaa atccgtccaa attttgggct cgctctacaa
     2941 gaccttggtg caattggccc aggagaaggg cgtggatctg gtggtcgaca aatcggccat
     3001 tctctacggt cagaacgcca tcgatttaac ggaacccttg gcccggcggt tgcgggggct
     3061 tccggacgac ggaaaaccct gaggactccc acgtgaaatt aacggtaacg caaattgcgg
     3121 aattgacggg cggcgcggtt cgcggcgacg gcgcgacggt tattgaaggc gcggccgggt
     3181 tggccgacgc caccccccgg gacatcacgt ttctgggcaa cgacaaatac caatcccagt
     3241 tggaaacgac ccgggccggg gcggtgctcg tggctcccgg cgtcgacacc gggtcccgcc
     3301 cggcggtcgt ggtcaaaaac ccgccctacg gctgggcccg ggttctggag gttttggaaa
     3361 aggaacgcac gcggcgaccg gcgggcatcc atccgacggc ccaggtggcc cccaccgccc
     3421 gggtcgggaa gaacgtgacc atcggcgcct tcaccgtggt ggaggacggc gcggtgatcg
     3481 gtgacaacag cgttttgtac gcccacgtgt atgtcggttt cgacgtccgc atcgggcgcg
     3541 attgcctgat ttatccccgc gcgaccctgc gcgagcgcgt cgccctgggc gaccgggtgg
     3601 tgttgcagcc cggcgcggtg atcggatgcg acggtttcgg ctttaccgta cacgaaggtc
     3661 ggcattacaa aattccccaa gtggggacgg tggagatcgg cgacgacgtg gagatccagg
     3721 ccaactcgac gatcgaccgc gcggccgtcg gcgtgacgaa gatcggccga ggcaccaaga
     3781 tcgacaacct ggtccaaata gcccacaaca ccgagatcgg ggagcattgc ctcatcgtcg
     3841 ctttgaccgg catcgcgggt tccgtcaagg tcggccggta cgtcaccctg gcggcccagg
     3901 tcggggtggc ggggcacctc agcatcgccg acggggtgac cgtggcggcc cggggcggcg
     3961 tgacgcagtc cctgaaaacc ccccgggaag tcctctgggg ccttccggcg caaccgattc
     4021 gcgacgaact caaatgccag gcggctctcc gacgtctgcc ggcggtgttg gaggaatggc
     4081 gacaatttaa aaagaaagga tccccctccg cgtgaaactg ttttccaccg gggaacgcca
     4141 acgcaccatc gcccgggagg cggtgtacaa aggcgtcggc cttcacaccg gcaacaaatg
     4201 ccacttgatt tttaagcccg cccccgcggg ccagggcgtc gtttttgtcc gcaccgattt
     4261 gccgggaaag ccgcgcctgc ccgcccatca ttccattgtc tccagcgtcg tgcgcggcac
     4321 cactttgagc cttgacgggg agaaaaacca cgaggcccgg gtccacaccg tggagcacgt
     4381 gttgtccgcc ctctacggcc tgggcatcga caacgcggtc atcgaggtga acgccaacga
     4441 accccccgtg gcggacggca gcgcccggcc cttttgggac gcgctggaag ccgccggcgt
     4501 gaccgagctg gacgaaccgc ggcgattttt ccagcccgat cccctggaat acaccgcggg
     4561 cgaaacccgt tacgaggtcg agccggccga cgagctgatc atcgagacaa acatccattt
     4621 caaacacccc ttgatcgggg agcaacgccg ggagttcacc gtgacccggg agtcctattt
     4681 gaacgaaatc tcccgggccc ggaccttttg cttcgatttc gaagtggaag ccctgaaacg
     4741 ccaagggctg gcccggggcg ggtccctgga caacgccgtc gtggtcggca tggaccgcat
     4801 tcacaacaag gaaaaatccc tccgctaccc ggatgaattc gtccggcaca agaccctcga
     4861 tctcatcggc gacctctttt tactgggcgc tcccctcaag gcccgcatca aagcccaccg
     4921 cgtgggccac ggacacaacg tcaatttggt caagaaattg gccgcgcaga tgaccggcga
     4981 agcaccgtcc cagacggcgg tcacgtcgaa ttactgattt aattcccgga ggagtcaccc
     5041 ccatggacaa cgtcgcgccc cgcaccctta ccaccgtcga gattatgggg gccattcccc
     5101 accgttaccc gatcctgatg atcgacacgg ccacgatcac cgagcccgaa aagaaaatca
     5161 ccgggttcaa gtccgtttcc gccaacgagc cgtttttcca gggccatttt cccggacgtc
     5221 ccatcatgcc gggcgtcctc attgtcgaag ccatggccca atccgcctgc gtgctctttt
     5281 tgtccaaacc ggaactcaag gataaattgg cgttcttt
//
LOCUS       DAOQLS010000046         5296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7072_length_5296_cov_4.351460, whole genome shotgun sequence.
ACCESSION   DAOQLS010000046 DAOQLS010000000
VERSION     DAOQLS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7072_length_5296_cov_4.351460"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PK362_01470"
     CDS             complement(<1..1032)
                     /locus_tag="PK362_01470"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNC73543.1"
                     /translation="MLAAPNAFKGSLTAGDAARALARGARKAVRPAPQIRLCPLADGG
                     DDTLEVLSGVFGARPRRARVTGPLGRPLQARWAYASRRRLAVIEMARSSGLALVPPSS
                     RNPEKTTAFGTGELLRAALDAGARTVYLALGGSATVEGGVGVLQALGATVFLREGDRS
                     SPLTRPFVGGDFSRLASLDVEGLDPRLKKIRLVLLTDVRNPLLGAGGAARVFGPQKGA
                     TPAQVRRLEKGLALLERWAGKGAGPGGGAAGGVAAGLVFWAGARVLAGASFVFDAVNL
                     KRHVRWADVVLTGEGRLDDTSFGGKTVGGLANRCRREKKPLAIFAGSATPGARRRGAR
                     LGAAVVPLAP"
     gene            complement(1043..2083)
                     /gene="alr"
                     /locus_tag="PK362_01475"
     CDS             complement(1043..2083)
                     /gene="alr"
                     /locus_tag="PK362_01475"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNC73544.1"
                     /translation="MKPLRWVEIDLGAIAANFRAVRRLVGPSAPVTAVVKGDGYGHGA
                     VQVAKTCLEAGARDVAVTYLEEALPLRRAGVRAPLLVMGPLDPAEAGLAAKENLSVMV
                     DNEPLLKALARRGRARPVSVQVKVDLGLRRWGIPLEALADFLKKIEALPGLRLDGVFA
                     HPGYMADKNAGEVDRRLRAFVAAARPLMKPFPKARLHGADSAVLLDFPEFRLDGVRVG
                     NLLYGMNPTRKDLSLKNPWFPRTRVVRVIRANPGDTIGYGSQYIAEKTLTLGTLPVGY
                     AHGFTLELAPARGNPYWVTIEGVRCPVIGRVGMSHTVVDLSGLPRPPREGDIAALPLR
                     RTASALWEKVYI"
     gene            complement(2080..3180)
                     /locus_tag="PK362_01480"
     CDS             complement(2080..3180)
                     /locus_tag="PK362_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNC73545.1"
                     /translation="MNEPAGSPNLNTPTPPAPLFAPGAPKGHPFQTEGWAEVKRRMGW
                     RSHFLEVTREGAVRAALRVLEKDFPKIGYRFFYAPRGPILETGWTDADLDKLFRRAET
                     LARERRAVFLKIDPDVPGPEEVLTGALRRNGFRLAPAVGSLSGVQPRCVFRLDLRPTE
                     EALLAGMDQKARYNIRLAEKKGVTVRPVSDRKDVETFFRLLQETAVRDKFLIRPLRYL
                     LDIQEQFESRGDAQFFLAEREGQAIAGALALKSGPLCLYAYGASANTGRQYMPNHLVQ
                     WTMVRWAKAHGCLVYDFRGVPSNPLPDDPLFGLFRFKKGFGGVFTEFIGEFDRVYVPW
                     LYWLWIYGWPLFKKIRHLLLFRRTSRTAGESD"
     gene            complement(3201..4292)
                     /locus_tag="PK362_01485"
     CDS             complement(3201..4292)
                     /locus_tag="PK362_01485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC73546.1"
                     /translation="MTASRSVVHVVPGLGYGGTEFVVLRLAEAERARGWDSRVVSLKR
                     SGALFPAFEKAGVPARPLGPSDFRGPAPGTVVGWLYNGNLAAGALALFRPGTGLVWNL
                     MQANLSAAVNRWPTRAAMRLGAALSAGRPDAVVCNSEAARTAHAAAGYAAAKMSVIPN
                     GIDTELYRPSENARTGVGPVVIGHLARWDPQKDHATFVRAVGRLLRRGVDVRARLAGP
                     GVTADNPDLTRWIAETGRPECFERLGALDQTVPFYQSLDVFCLSSVGESSPYALAEAM
                     ACGLPAAATDVGDCRAVLNDGDALAPAGDDDALSRALERWARLGPEERRRKGREARGR
                     IVEHYSFRRMVDAYLDLYQTVAARRDGRG"
     gene            complement(4289..5161)
                     /locus_tag="PK362_01490"
     CDS             complement(4289..5161)
                     /locus_tag="PK362_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC73547.1"
                     /translation="MRIWVTGSGGYIGSRLLKSLRRRFPRAVVQGDRRGPGPAPRFKS
                     IDDVGARLARFRPTHVVHCLGRTRPGTLQQLNAAHVAPTFYLLEALRRLPAPRPRVLL
                     VGSAAEYGASRRRLTESAPARPQTDYGVSKLLQTRLGLAFHALGVPVIVARLFNLYAP
                     DAPATFAVPRVWNLLRRKSKGRKILETGPQNAVRDFLALPDALEALALLATRGKPGEI
                     YNVCSGRGLRMGELFDAMAEGAGVSVGWKTTGTGSGKSAASVAVGDPAKIRRRTGWRP
                     RLDVLALARAGKKS"
BASE COUNT          888 a         1815 c         1845 g          748 t
ORIGIN      
        1 gggcgccagg ggaaccaccg ccgctcccag acgggcgcct cggcgccggg cgccgggcgt
       61 cgcgcttccg gcgaaaatcg ccaagggttt cttttcccgc cggcaccgat tcgccagccc
      121 gcccacggtt tttccaccga aggacgtgtc gtccagccgc ccctcgcccg tcagaacgac
      181 gtcggcccac cgcacgtggc gtttcaaatt caccgcgtcg aacacgaagg aggccccggc
      241 caacacccgg gcgcccgccc agaaaacaag gcccgccgcc accccgccgg cggcgccgcc
      301 gcccggcccc gcgcctttcc ccgcccaacg ctccaggagg gcgagtccct tttccaaccg
      361 gcgcacctgg gccggggtcg cgcccttttg gggtccaaag acccgggccg ctcccccggc
      421 cccgagcaat gggtttcgaa cgtccgtgag caggaccagt cgaatttttt tcagccgcgg
      481 gtccaacccc tcgacgtcca gggaggccag gcgggaaaaa tcgccgccga caaagggccg
      541 ggtcaggggc gacgaccgat cgccttcccg caggaaaacc gtcgccccca gggcctgaag
      601 aacgcccacc ccgccttcga cggtcgcgct tcccccgaga gccagataaa cggtgcgcgc
      661 gccggcgtcc aaagcggcgc gcaacagttc gcccgtcccg aaggcggtgg ttttttcggg
      721 atttcgggag gaaggtggaa cgagggccag gcccgaggac cgggccatct cgatcacggc
      781 cagtcgtcgc cgggacgcgt aggcccagcg ggcttgcagg ggccggccga gggggcccgt
      841 gacccgggcg cgccgggggc gggcgccgaa gaccccggag agaacttcca gggtgtcgtc
      901 gccgccgtcg gccagcggac acaatcgaat ctggggggcg gggcgaacgg cttttcgagc
      961 gccccgggcc agggcgcggg cggcgtcccc ggcggtcagg cttcctttaa aggcgttggg
     1021 cgccgccagc acccggagcc gactatatat agaccttctc ccaaagggcc gaggcggtgc
     1081 ggcggagggg cagggcggcg atgtcgccct cccggggcgg ccgggggagg cccgagagat
     1141 ccaccaccgt gtgactcatg cccacgcgcc cgatcaccgg acagcgaacg ccctcgatgg
     1201 tgacccaata gggatttccc cgggcggggg ccagctccag ggtgaagccg tgggcgtaac
     1261 ccaccggcag ggtgccgagg gtcagggttt tttcggcgat gtattggctg ccgtagccga
     1321 tggtgtcgcc ggggttcgcc cggatcacgc ggaccacccg ggtccggggg aaccaggggt
     1381 ttttaaggga cagatccttc cgcgtcgggt tcatcccgta gagcaggttg cccacgcgca
     1441 cgccgtccaa ccggaattcc ggaaaatcca ggagcaccgc gctgtccgct ccgtgcaggc
     1501 gggccttggg aaagggcttc atcagcggcc gggcggcggc cacgaaggcg cgcagacgac
     1561 ggtccacttc gccggcgttt ttgtccgcca tgtagcccgg gtgggcgaaa acgccgtcca
     1621 gccggaggcc cggcagggct tcgatctttt tcaggaaatc cgccagagcc tccaggggga
     1681 ttccccagcg gcgaaggccc aaatccactt tcacctgaac cgacaccggc cgggcacgac
     1741 cccggcgggc cagggccttg agcaggggct cgttgtccac catgacggac aaattttcct
     1801 tggcggccag gccggcctcc gccgggtcca ggggacccat gacgaggagg ggcgcccgca
     1861 cgcccgcccg gcgcaggggc agggcttctt ccaaataagt caccgccacg tcccgggccc
     1921 cggcctccag gcaggttttg gccacttgca ccgccccgtg gccgtagccg tcgcccttga
     1981 ccaccgccgt cacgggcgcc gacggaccga cgagacgccg aaccgcccgg aagttggcgg
     2041 cgatggcgcc caggtcgatc tccacccagc ggaggggctt caatcggact ccccggccgt
     2101 gcggctcgtc cgtcggaaca acaggaggtg gcggattttc ttaaagaggg gccagccgta
     2161 gatccacaac caatagagcc acgggacgta gacccggtcg aactctccga taaattccgt
     2221 gaagacgccg ccgaagcctt ttttaaaccg gaagaggccg aagagcggat cgtcgggcaa
     2281 agggttgctc ggcacgccgc gaaagtcgta gacgaggcag ccgtgggcct tggcccagcg
     2341 gaccatggtc cattgaacca ggtggttggg catgtattgg cggccggtgt tggcgctggc
     2401 gccgtaggcg tagaggcaca gggggccgct cttcaaggcc agggccccgg cgatggcctg
     2461 gccctcgcgc tcggccagaa agaattgggc gtcgccccgg gactcgaatt gctcctgaat
     2521 gtccaacagg tagcgaaggg ggcggatcag gaatttgtcc cgcacggcgg tttcttgaag
     2581 gaggcggaaa aaggtctcca cgtccttccg gtccgagacg gggcggacgg tgacgccctt
     2641 tttttcggcc aggcggatgt tgtagcgggc cttctggtcc atgcccgcca agagcgcctc
     2701 ctccgtcggc cgcaggtcca gacggaaaac gcaccggggt tgaacgccgg acaaactgcc
     2761 cacggcggga gccaaccgaa acccgtttcg ccgaagggcc cccgtgagaa cctcctccgg
     2821 gccgggcacg tccgggtcga ttttcaggaa caccgcccgg cgttcccgag cgagggtctc
     2881 ggcccggcgg aaaagcttat ccaaatccgc gtcggtccac cccgtctcca ggatcggccc
     2941 ccggggcgcg tagaaaaacc ggtaaccgat tttggggaaa tccttttcca acacccgcag
     3001 ggcggcccgc accgcgccct cccgggtcac ttccaaaaag tgcgaccgcc agcccatgcg
     3061 gcgtttgacc tcggcccacc cttccgtctg aaaaggatgg cccttgggcg ccccgggggc
     3121 gaagagcggc gcgggcggcg tcggggtgtt taaattcggg gagccggcgg gttcgttcac
     3181 aggcgaggat tttgccatat tcaaccgcgc ccgtcccgcc gggcggcgac ggtttggtag
     3241 agatccaaat aggcgtccac cattcggcgg aaggaataat gttcgacgat ccgcccccgg
     3301 gcctcccggc cctttcggcg gcgctcctcc ggccccaggc gcgcccaacg ctccagggcc
     3361 cgggacaggg cgtcgtcgtc gcccgccgga gccagggcgt cgccgtcgtt caacaccgcg
     3421 cggcaatccc ccacgtcggt ggccgcggcg ggcaggccgc aggccatggc ttcggccagg
     3481 gcgtagggcg agctctcccc caccgacgac agacaaaaga cgtccaggga ctgatagaag
     3541 ggcacggttt gatccagggc gcccaggcgc tcaaagcatt cgggacggcc cgtttcggcg
     3601 atccagcgcg tgagatcggg attgtcggcc gtcacgccgg gccccgccag gcgggcgcgg
     3661 acgtccacgc cccgccgcaa aaggcggccc acggcccgca cgaaggtggc gtggtctttt
     3721 tgagggtccc accgggccag atggccgatg acgacgggcc cgacgccggt ccgggcgttt
     3781 tccgacgggc ggtagagttc ggtgtcgatc ccgttgggga tgacgctcat ttttgcggcg
     3841 gcgtagcccg ccgcggcgtg ggccgtccgg gcggcttcgg aattgcagac gacggcgtcc
     3901 ggccgtccgg cggagagggc cgcgccgagc ctcatggcgg cccgggtggg ccagcggttc
     3961 acggcggcgc tcaagttcgc ctgcatgagg ttccacacga ggcccgttcc ggggcggaac
     4021 agggccaggg ccccggccgc gaggttgccg ttgtagagcc agcccacgac ggtgccgggg
     4081 gccgggcctc gaaaatccga cgggcccagg ggccgggccg ggacgccggc cttttcaaaa
     4141 gcgggaaaaa gcgcgccgct ccgcttgagc gacacgaccc ggctgtccca cccccgggcc
     4201 cgctcggcct cggccaaacg caacaccaca aattccgtgc cgccgtaccc caacccggga
     4261 accacgtgga ccaccgaccg ggaggcggtc acgatttttt ccccgcccgg gccaacgcca
     4321 gcacgtcgag gcggggccgc caaccggtcc gtcggcgaat tttggcggga tcgcccacgg
     4381 cgacggacgc ggccgacttg ccggacccgg tgcccgtcgt tttccacccg acggaaaccc
     4441 ccgcgccttc ggccatggcg tcgaacaact cgcccatgcg aaggccccgc cccgaacaaa
     4501 cgttgtagat ctccccgggt tttccccggg tcgccaaaag cgccagggcc tccagggcgt
     4561 ccggcagggc caggaaatcc cgcacggcgt tttgcggacc cgtttcaaga atcttccgcc
     4621 ccttggattt tcgtcgaagg agattccaca cccggggcac ggcgaaggtg gcgggggcgt
     4681 cgggggcgta caaattgaag aggcgcgcca cgatgaccgg cacgcccagg gcgtggaacg
     4741 ccaaccccag ccgggtttgg agaagcttgg aaacgccgta atcggtttgg ggacgggcgg
     4801 gggcgctttc ggtcagtcgt cgtcgggagg cgccgtattc cgcggcggag cccaccagga
     4861 gaacccgggg ccgcggggcg ggaagacgac gcagggcttc cagaagataa aacgtgggcg
     4921 ccacatgggc ggcgttcaat tgttgaaggg tgccgggccg ggtccgaccc aggcagtgaa
     4981 cgacgtgggt gggccgaaac cgcgccaacc gcgcgccgac gtcgtcgatg gatttgaacc
     5041 ggggggcggg gcccgggccc cgacggtcgc cttgcacgac cgcccggggg aaacggcggc
     5101 gaaggctttt cagcaggcgg gaaccgatgt agccgccgct ccccgtgacc caaattcgca
     5161 cgacgggtta aacggaggtg atcgacgggc ccggggtctt cgtgaaaagt tcctgggcct
     5221 ggcggtaatc gtcggggcgg ccgatgtcca accactggca ccggcttggg taggcgacca
     5281 cccgctcgcc cgcctt
//
LOCUS       DAOQLS010000047         5281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7112_length_5281_cov_4.966896, whole genome shotgun sequence.
ACCESSION   DAOQLS010000047 DAOQLS010000000
VERSION     DAOQLS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7112_length_5281_cov_4.966896"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..440)
                     /gene="cutA"
                     /locus_tag="PK362_01495"
     CDS             complement(102..440)
                     /gene="cutA"
                     /locus_tag="PK362_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNC73548.1"
                     /translation="MKPVLLLVSAPKGPAGRRLARALVEERLAACVTAVPGAASVYRW
                     KGRVERTAEELLFIKSARPRLPALFRRIKALHPYDVPEILVWPVAGGNADYLAWLGAA
                     VAASGRARIR"
     gene            complement(437..2344)
                     /locus_tag="PK362_01500"
     CDS             complement(437..2344)
                     /locus_tag="PK362_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC73549.1"
                     /translation="MAKGARDVYFDTVKALERRAAALEHIALYARSPAKLEAVLSGIL
                     ESAMELVGADAGTVYLANPATGQFDFAAARWASLSAAESADREKSLKAAPVKLNEGIV
                     GQVFQNREPVTVPDVTKSAVFRKDVADVAHYEIRNLLAVPLQLDETPLGVLELFNKTP
                     KGVFASSDLELAVSLAHLTALVIDLHRQRAAAKALPTPAPKPVEPETPKTNPAAAAEL
                     QEARRALRDAQAQLAETRKLLDAALQVQEQNTRRVKDLGDENERLKAMADAATPPQQM
                     TRLLKSVEPFAFTLSLDRVLKNFMELSARLVNAQAVQVFLWDARAERLSLAASTAAAA
                     GKGVSLAFKKGEGVAGAVAESLEILRVTDVTKEDRFSKTIDEAPGILTRSLLVAPLAA
                     QGRLLGVIELINKKNGGSFLEEDVVGLGGLALMGSAALEKTLLHRELVDAHRSVLGLV
                     ADLIETRTALDQGRHERIRALSQALAENLGFREAELRDLDWAALLFNIGLATLPADLT
                     TKKSDLTPAEREALSKVSRVSAAVLNPVPALAEAARLVKHVTERWDGLGDPDRLSAGG
                     IPYGSRVLAVVDAFDALTTGAGGRKTLPPDVALKELESGAGKLFDPACVESFARLYKS
                     GRFKAALNRPK"
     gene            complement(2337..3032)
                     /locus_tag="PK362_01505"
     CDS             complement(2337..3032)
                     /locus_tag="PK362_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73550.1"
                     /translation="MVRRLRERHGPPGWWPVAPAPGRPARYDPRWTRGFLTPAQQDEV
                     AIGAVLTQNTAWTNVEKALAALRRARALRLDRWRVLRPALLEKLVRPSGFFRQKTKRL
                     RGLAAHLGDRWGGRLGRLLSRPLPEARAALLALNGFGPETVDSLLLYAGRHPVFVVDA
                     YTRRLAHRLRLFRYNNTDMIQSYFHRRLPRRVPLYREAHALIVAHAKSHCRTVPRCGD
                     CPLRARCPEASRG"
     gene            complement(3053..3586)
                     /locus_tag="PK362_01510"
     CDS             complement(3053..3586)
                     /locus_tag="PK362_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNC73551.1"
                     /translation="MIRALGRDTPVVHPTAYVDPSATVIGRVRIGPHASVWPGAVLRG
                     DIEPIVLGEGTNVQDNAVIHTDHGVPTILGNGITVGHGAILHSCTVEDDALIGMGAIV
                     LGRAVVQKGALVGAGALVPPGGHVDAGRLALGSPARSVRKLTDGEAREIRENARRYVA
                     LAERYARSPRRRPSGKK"
     gene            complement(3583..4533)
                     /gene="argF"
                     /locus_tag="PK362_01515"
     CDS             complement(3583..4533)
                     /gene="argF"
                     /locus_tag="PK362_01515"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNC73552.1"
                     /translation="MTHAKHDLLSMAALSASDIDGLLTEARRLKARRRPEQRLAGKTL
                     GMIFEKPSTRTSVSFAVAMVELGGHALTLDARNLQIGRGETMADTARTLSRYLSGLMM
                     RANRHADVEELALHADIPVINGLTDKEHPCQVLADLQTALEAFRLKKTADLRRLRFAY
                     VGDGNNMTHSWMLAAGVLGLQFVAATPAGYAPDADVQKKAAALARASGGSVEVTRDPA
                     AAVRGADVVYTDVWTSMGQEAEAEKRRSVFRPYQINDQLLSRAAPRAVVMHCLPAHRG
                     EEITAAVLEGPRSVVFDQAENRLHVQKAVLLHCLGGGRSR"
     gene            complement(4554..>5281)
                     /locus_tag="PK362_01520"
     CDS             complement(4554..>5281)
                     /locus_tag="PK362_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73553.1"
                     /translation="DGTHFERLTNDRSIALFPKFSPDGEWIVFTSYKNGGPEVHVMRS
                     NGKDRRAVCRYDGLNSAAAWAPDGKSLIATLSLGREPNLHQVDLDGRVIKTLTNSSAA
                     DTAPTLSPDGLRLAFTSDRPGPPQIYAMDTSGANLQRLTTEGQCDSPGWSPLGHLLAF
                     TMSVKGNYDIYTLEVATGRTTRLTFGEGNNENAAWSPNGRHLVFTSSRRGRPELWVMN
                     ADGSSPRRLGNIPGRSFSPDWGR"
BASE COUNT          890 a         1675 c         1856 g          860 t
ORIGIN      
        1 ccagcaagcg ccgccgccgg ggcgaggcgg acgccaacac gagggggacg ggagaaccgg
       61 ggggtcgaaa ccggggcgtc atcgggcggg aaccgcggaa ctcaacgaat tcgggcccgg
      121 cccgaggccg cgacggcggc gcccagccaa gccaggtagt cggcgtttcc gccggccacg
      181 ggccacacca atatttcggg gacgtcgtag ggatggaggg ctttgattcg acggaatagg
      241 gcgggcaggc ggggccgggc ggatttgatg aacaacaatt cttccgccgt ccgctcgacg
      301 cggcccttcc agcggtacac ggacgcggcc cccggcacgg cggtgacgca ggccgccaaa
      361 cgctcctcca ccaaggcccg ggccaggcgc cgaccggcgg ggcccttcgg cgccgaaacc
      421 aggagaagga ccggtttcac ttcgggcggt tgagggcggc tttgaaccgg cccgatttgt
      481 aaagtcgggc gaaggattcg acgcaggccg gatcgaacaa tttgcccgcg ccgctctcca
      541 gttcctttag ggcgacgtcg ggcggcaagg ttttgcgacc cccggcgccg gtggtcaaag
      601 cgtcaaaggc gtcgacgacc gccagcaccc gggacccgta cgggatgcct cccgcggaca
      661 gccggtccgg atcccccaga ccgtcccaac gttccgtcac gtgtttcacc agccgcgcgg
      721 cctcggccaa ggcgggcacc gggttgagca ccgccgcgct cacgcgcgac actttggaca
      781 gggcctcccg ttcggccggg gtgagatccg attttttcgt cgtgagatcc gccggcaggg
      841 tggccaagcc gatgttgaaa agcaacgccg cccaatccaa gtcccggagc tccgcctccc
      901 ggaaacccaa attttcggcc aaggcctggg acagggcgcg gatccgttcg tggcggcctt
      961 gatccaaggc cgtccgggtt tcgatcaaat ccgccacgag gcccaagacg gaacgatggg
     1021 cgtccaccaa ttcccggtgc aaaagggttt tttccagggc cgccgagccc atgagggcca
     1081 gcccgcccaa gccgacgacg tcctcctcca aaaacgaccc gccgttcttt ttgttgatca
     1141 gttcgatgac gcccaaaaga cgaccctggg ccgccagcgg agcgaccaac agcgaccggg
     1201 tcaggatccc cggcgcctcg tcgatggttt tggaaaaacg atcttccttg gtgacgtccg
     1261 ttacccgaag gatttccaaa ctttcggcca ccgccccggc caccccttcg ccctttttga
     1321 acgccaggga cactcctttc ccggcggccg cggcggtgga ggcggccagg gacaatcgct
     1381 ccgcccgcgc gtcccagagg aacacctgga cggcttgggc gttcaccagg cgcgcggaca
     1441 gttccatgaa attcttcaac acccggtcca gggaaagcgt aaaggcaaaa ggttcgacgg
     1501 atttgaggag acgggtcatc tgctggggcg gcgtggcggc gtcggccatg gctttcaagc
     1561 gttcgttttc gtcgcccagg tccttcaccc ggcgcgtgtt ttgttcctgg acctgcaggg
     1621 ccgcgtccag caatttacgc gtttcggcca attgggcctg ggcgtcccgg agcgcccgcc
     1681 gggcttcttg aagttccgcc gcggcggccg gattggtctt gggcgtttcc ggttcgacgg
     1741 gtttgggcgc cggcgtggga agggccttgg cggccgcccg ctggcgatgg agatcgatca
     1801 ccagggccgt caaatgggcc aaggacacgg ccaattccaa atcgctggag gcgaaaaccc
     1861 ccttcggggt tttattaaag agttccagga cccccagggg ggtctcgtcc aattggaggg
     1921 gcaccgccaa caaatttcgt atttcgtaat gggccacgtc ggccacgtct ttgcggaaca
     1981 cggcgctttt ggtcacgtcg ggaaccgtga cgggttcccg gttttgaaac acctgtccga
     2041 cgatgccttc gttgagtttc acgggcgcgg ctttgaggga tttttcacgg tcggcggatt
     2101 ccgcggccga gagggaggcc caacgggcgg cggcgaagtc gaactgcccg gtcgcggggt
     2161 tcgccaggta aacggtgccc gcgtcggcgc ccaccaactc catcgcgctt tccaagattc
     2221 ccgagaggac cgcctccagt ttggcggggg accgggcgta gagcgcaatg tgttccaggg
     2281 cggcggcccg gcgttcgagg gccttgacgg tgtcgaaata gacgtcccgg gcccccttag
     2341 ccacgggagg cctcgggaca gcgcgcgcgg agggggcagt cgccgcaccg gggcacggtc
     2401 cggcaatggg acttggcgtg ggcgacgatc agcgcgtggg cctcgcggta caacggcaca
     2461 cgccggggga ggcggcggtg gaaataggat tgaatcatgt cggtgttatt gtagcgaaaa
     2521 agtcgaaggc ggtgtgccag gcgacgcgtg tacgcgtcca ccacaaacac cgggtggcgt
     2581 ccggcgtaca gcaagaggga atcgacggtt tcggggccga acccgttcag ggccaggagc
     2641 gcggcgcggg cctcggggag cggtcgcgag agcaatcggc cgagacgtcc cccccaccgg
     2701 tcccccaggt gggcggccaa gccgcgcaag cgcttcgttt tctgacggaa aaaacccgaa
     2761 ggacgaacaa gtttttccaa gagggccggg cggagaaccc gccaccggtc gagccgaagg
     2821 gcccgggccc gtcgaagggc ggccagcgcc ttctcgacgt tggtccaggc ggtgttttgg
     2881 gtgagaacgg cgccgatggc cacttcgtcc tgctgggccg gggtcagaaa accgcgggtc
     2941 caacggggat cgtaacgcgc gggccggccg ggggccgggg ccacgggcca ccaccccggg
     3001 gggccgtggc gctcccgcaa acggcgcacc acccaggcgg gcgtggcggg gattattttt
     3061 ttcccgaagg tcgacgtcgg ggcgaccggg cgtaacgttc ggccaacgcc acgtaccgcc
     3121 gggcgttttc ccgaatttcc cgggcctcgc cgtcggtcag cttgcgcacc gagcgcgcgg
     3181 gagaccccaa ggccagccgg ccggcgtcca cgtggccgcc cggcggcacc agggccccgg
     3241 cgccgaccaa ggcgcctttt tgcaccacgg cgcgccccaa cacgatggcg cccattccga
     3301 tcagcgcgtc gtcttcgacg gtgcacgaat ggagaatggc cccgtggccg acggtgatgc
     3361 cgtttccaag aatcgtcggg acgccgtggt cggtgtggat cacggcgttg tcctgcacgt
     3421 tggtgccttc gccgaggacg atgggctcga tgtcgccccg aagcacggcg cccggccaaa
     3481 cggaggcgtg gggcccgatg cgcacccggc cgatcacggt ggccgacggg tccacgtagg
     3541 ccgtgggatg aaccacgggc gtgtcgcggc ccagggcgcg aatcatcggg accgcccgcc
     3601 gcccaggcaa tgaagcagga cggctttttg cacgtgcagg cggttttcgg cttgatcgaa
     3661 aaccacggac cgggggcctt ccaaaacggc ggccgtgatt tcctcgcccc ggtgggcggg
     3721 caggcaatgc atcacgacgg cccggggcgc cgcccgggaa agcagttgat cgttgatctg
     3781 gtacggccgg aaaaccgagc ggcgtttttc ggcttcggcc tcctgcccca tggaggtcca
     3841 cacgtcggtg tacaccacgt cggcgccgcg gacggcggcg gccggatccc gggtcacttc
     3901 gacggacccc ccggacgccc gggccagcgc ggcggccttt ttttgcacgt cggcgtccgg
     3961 ggcgtaaccc gccggggtcg ctgcaacaaa ttggaggccc aacacgccgg cggccagcat
     4021 ccaggaatgg gtcatgttgt tgccgtcgcc cacgtaggcg aaacggagtc gccgaagatc
     4081 ggccgttttt ttcagccgga aagcctcgag ggccgtctga aggtcggcca gcacttggca
     4141 gggatgctcc ttgtccgtca agccgttgat cacggggatg tcggcgtgga gcgccagttc
     4201 ctccacgtcg gcgtggcggt tggcccgcat catgaggccc gacaaatagc gggacagggt
     4261 gcgggccgtg tcggccatgg tttcgccccg cccgatctgc aaattgcggg cgtccagcgt
     4321 gagagcgtgc ccgccgagct ccaccatggc cacggcgaag gacacggagg tgcgggtcga
     4381 gggcttttca aaaatcatgc cgagggtttt ccccgcgagc cgttgctcgg ggcgacggcg
     4441 ggctttgagg cggcgcgcct cggtcagcaa gccgtcgatg tccgaagcgg acaaggcggc
     4501 catggaaagc aaatcgtgtt tggcgtgggt cataaaaatc cttcgtggga tggtcagcgg
     4561 ccccaatcgg gggagaacga acggccggga atgttgccca aacgccgggg gctggaaccg
     4621 tcggcgttca tcacccacaa ctccggtcgt ccccgccggg aactggtgaa caccaggtgg
     4681 cgcccgttgg gcgaccaggc ggcgttttcg ttgttgcctt ccccgaaggt cagccgggtg
     4741 gtgcgtccgg tggcgacttc cagggtgtaa atatcgtagt tgcccttcac gctcatggtg
     4801 aaggccagca ggtggcccag gggcgaccag ccgggggaat cgcattgccc ttcggtcgtc
     4861 aagcgttgga ggttcgcgcc gctggtgtcc atggcgtaaa tctgcggagg ccccgggcgg
     4921 tccgaagtga aagcgaggcg gaggccgtcg ggcgagagag tcggcgcggt gtccgcggcc
     4981 gaggagttcg tcagcgtttt gatcacccgg ccgtccaaat ccacctggtg caaattgggc
     5041 tcccgcccca gggacagggt ggcgatcagg gattttccgt cgggggccca ggccgccgcg
     5101 ctgttcaatc cgtcgtaacg gcagaccgcg cgccggtctt tcccgttgga ccgcatgacg
     5161 tggacttcgg gaccgccgtt tttgtaggac gtgaagacga tccactcccc gtccggggag
     5221 aatttcggga agagggcgat ggagcggtcg ttcgtcagcc gctcgaaatg ggtgccgtcg
     5281 t
//
LOCUS       DAOQLS010000048         5259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7141_length_5259_cov_4.347041, whole genome shotgun sequence.
ACCESSION   DAOQLS010000048 DAOQLS010000000
VERSION     DAOQLS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7141_length_5259_cov_4.347041"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PK362_01525"
     CDS             <1..563
                     /locus_tag="PK362_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNC73554.1"
                     /translation="VSLMKIANDVRWLSSGPRCGIGEIEIPSLQPGSSIMPGKVNPVI
                     PEAVAMAAAQVIGADAAITVGGLSGNFELNVMKPVIAHNLLSALSIVANVVRLFTEKC
                     VKGIKANRDIAEGFIEKSLAMCTALAPKLGYDKAAEVAKEAYASGKTVRQVVLEKKML
                     PEKELAALLNARAMTEPGMTLDGAGG"
     gene            complement(560..2164)
                     /locus_tag="PK362_01530"
     CDS             complement(560..2164)
                     /locus_tag="PK362_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC73555.1"
                     /translation="MTVSRKIVPGPATAALAVLALVFAVYGRGLFYSFVYDDRWTIVE
                     NVAVQRPTNLARFFLDRDTVAVPATGMGRTIYRPLPTLTFALDRALGARAPWRFRLAN
                     LLLHVTNGVLLWGLFRSLGLSFGASLAGSAAFLLHPAQVESVQWVTQRSNLMCLAGLL
                     TALHFHRRGSAWSLAGLALALLSKETAVVAIALWPLFLWLEGPRIPPFQKGSARARWL
                     AAGGLVAAYLLLRGTVIGGFAQRDFRGGSWTGGLLLGTLSLWEYVKILVWPLGLRVSR
                     RQYIEDPWTSPWTLVGLGTAIVTALVLFYLWRRHRRAFFWAGWAPLTLLPVLGLAPTD
                     TFVAERFLYVPLAGVGGVAALLWDRFGAAGRRARGWARGVLAAALALWAGLSWAQVGV
                     WRDELSLWTSTANRDPEHGFSWLCRAQALAERGRFPEAEAEYLHALRVGLTRGPAAAA
                     LTNLSDLRLRAEDPAGALVWADKALIAAPASAPALVNRARALVGLNRKSEALGVVDRL
                     TSDHPPAVQWEPLRRWVENAPPGRRP"
     gene            2222..4129
                     /locus_tag="PK362_01535"
     CDS             2222..4129
                     /locus_tag="PK362_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73556.1"
                     /translation="MTRKDWIALGVFALALAGVFSGTWLTDRTFFARDLTFLFHPMHV
                     FTAQALQAGELPAWNPYVSGGVYWLGQPQTGLFSPGNLAFWVFPFVPGFKIFQAVHFL
                     LSGLGGYLWVRANRGARWAALSSALLWSLNGFWRARVEFPPLLNTLTWTPWALLFLGA
                     KPGWTSGAGLALAMSFALLGGHWPHVAWMGVALLALWAAAGGRRRGIVLMSAGVGAAI
                     FSAVQLLPGLDLLGQSARVQSGVAPAVADLHSLTVARWAGLLAPVFGDFPSDRYTGER
                     FFWLGCFYIGALALWAAGRGWRRSSPRHRATWAALFLLGGLFSLGNAVPLYAWAVDRV
                     PGLRGVRFPAQYAYWMVVSALSLLAVVGGGDFRRPWLRWGAVGIVAAELMFWGAGLQP
                     TLPGSFFHSRPSWIRGVQSSPGKLFLSPRAADSMSARGRTLEETWTALRHRLLDLGTL
                     PYRVANLNPSGFSLNPAGNDKALLALYAHPTPAAAEPDFDRLDVRWVAAPGALEVPGW
                     RLREDNPWRLYERSRPSGDGAVLSGATRAAWSRRIRALGRDEWTGAPAENATLVTRTL
                     WQKDWIAGTARGPLDVLDRDGLLSVRWDRPLDRFWARFAPSSLWWGILGTFAGTATAL
                     AFAFHRARSVR"
     gene            4126..>5259
                     /locus_tag="PK362_01540"
     CDS             4126..>5259
                     /locus_tag="PK362_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73557.1"
                     /translation="MKRALGLFAALTGLFTAPLIFGRGFVANAGDVYDYAAPFRAWGN
                     RELLGGRLPFWNPWIFGGTPFLASPQSALFYPGNTAMVSTHLTAGFRILIVLHLFLMG
                     IGYWMWLRSRGERWGGSALGAVLATFSFFTIAKVAAGHAIHLTGYAWTPYVFLLSEAV
                     ARRPDNRHLPWAAAAASGLQFLTGHLQVWLVTHLVVAGLLAGRRLADRRVARALGAAL
                     LFGAFLVAVQAFPTADFLPRTTRGLRGVLTDRDRYAFATSYSLELGRLWKGWTNPWAW
                     GALPKPPLFPEPPSVYFETATVFVGWGGLALAVVGLVLLLRRRQWRWPVLAAGSLMAA
                     LGKNGPVYPVLWRCLASLRVPARFYLGIHVALAAAAARGWSVLS"
BASE COUNT          783 a         1729 c         1779 g          968 t
ORIGIN      
        1 ccgtatcatt gatgaagatc gccaacgacg ttcgctggct ttcctccggc ccccggtgcg
       61 gcatcgggga aatcgaaatc ccgtcgcttc agccggggag ttcgattatg ccggggaagg
      121 tcaaccccgt gattcccgaa gccgtggcga tggccgccgc ccaggtcatc ggcgccgacg
      181 ccgccatcac ggtcggcgga ttgtcgggca acttcgaatt aaacgtcatg aagccggtca
      241 tcgcccacaa cttgctgagc gcgctttcca tcgtggccaa cgtcgtgcgc ctgtttaccg
      301 agaaatgcgt gaagggcatc aaggccaacc gcgacatcgc cgaagggttc atcgagaaga
      361 gcctggccat gtgcacggcc ctggccccca aactgggcta cgacaaggcc gccgaggtcg
      421 ccaaggaagc ttacgcttcc ggcaaaacgg tgcggcaggt ggtgttggag aaaaaaatgc
      481 tgccggagaa ggagttggcg gcgctcttga acgcccgggc catgaccgag ccgggcatga
      541 cgctcgacgg cgccgggggt taaggccgcc gtcctggcgg ggcgttctcc acccagcgcc
      601 ggaggggttc ccattggacg gcgggggggt ggtcggacgt caatcgatcg acgacgccca
      661 acgcctcgct ttttcggttg agaccgacga gggcccgggc ccggttcacg agcgcgggcg
      721 cggaagcggg agcggcgatc agcgctttat cggcccagac aagggcgccc gcgggatcct
      781 cggcccgaag tcgaaggtcc gataaattgg tcagcgccgc cgcggcggga ccgcgggtaa
      841 gaccgacgcg caaagcgtga aggtattccg cctcggcttc ggggaatcgg cccctttccg
      901 ccagcgcctg ggcccggcac agccacgaaa agccgtgttc ggggtcccgg ttggccgtcg
      961 aagtccagag cgatagttcg tcccgccaaa cccccacctg cgcccaggaa agccccgccc
     1021 aaagagccaa cgccgccgcc aagacgcctc gcgcccaacc ccgggcccgc cggcccgccg
     1081 cgccgaaacg atcccacaaa agggccgcca cgccccccac gccggcgagg ggcacgtaaa
     1141 gaaagcgttc cgccacgaag gtgtctgtcg gcgcaaggcc caaaaccgga aggagcgtca
     1201 gcggcgccca ccccgcccag aaaaaggcgc gccgatggcg acgccacaaa tagaacaaga
     1261 ccaaggcggt gacgatcgcg gtcccgagcc ccaccaacgt ccaggggctc gtccagggat
     1321 cctcgatgta ttgacgtcgg ctgacccgaa ggcccagagg ccaaaccaga atttttacgt
     1381 attcccagag ggaaagggtc ccgagcagga ggccgccggt ccagctcccc ccgcggaaat
     1441 cgcgctgagc aaatccgccg atcaccgtcc ctctcaagag gagataagcg gccaccaaac
     1501 ccccggccgc cagccatcgc gcgcgggcgg accctttttg gaaaggcgga attcgcgggc
     1561 cctcgagcca taagaagagc ggccaaaggg cgatcgccac gaccgcggtt tcttttgaga
     1621 gaagggccag ggcgagaccc gccagcgacc acgcggagcc tcggcggtgg aaatggaggg
     1681 cggtcaggag gcccgccagg cacattaaat tcgaacgctg ggtcacccac tggacggatt
     1741 cgacttgagc gggatgaaga agaaacgcgg cggaccccgc cagggacgct ccaaacgaaa
     1801 gtccgaggga gcggaacagc ccccagagaa ggacgccgtt cgtgacgtgc aggaggaggt
     1861 tcgccaagcg gaaacgccag ggggcccggg ccccgagcgc ccggtccagg gcgaaggtca
     1921 aggtcggaag cggacggtaa atcgtgcggc ccatgcccgt ggccgggacg gccacggtgt
     1981 cgcggtccag gaaaaaccgc gcgaggttcg tgggccgctg cacggcgacg ttctcgacga
     2041 tggtccagcg gtcgtcgtaa acgaaggaat agaaaagtcc ccgcccatac acggcgaaca
     2101 ccagcgcgag caccgccaga gcggccgtcg cgggccccgg gacgattttt cgcgagaccg
     2161 tcatgagagg ctattgtaat atatcgccca tgcgccgccc ccgccggacc cgctcccggc
     2221 catgacacgg aaagattgga tcgcgctggg cgttttcgcc ctcgcgctcg ccggggtgtt
     2281 ttccgggacg tggctgaccg accgaacttt cttcgcccgg gacctgactt tcctttttca
     2341 tcccatgcac gtgtttacgg cccaggccct tcaggcgggg gaattgccgg cctggaaccc
     2401 ctatgtctcc ggcggcgtgt attggctggg tcaaccccag accggtttgt tttcccccgg
     2461 caacctcgcc ttttgggttt ttcccttcgt gcccgggttc aaaatattcc aagcggttca
     2521 cttcctgttg tccggactcg gcgggtattt gtgggtgcgg gccaatcggg gagcccgctg
     2581 ggccgcgctg tcctcggctc tgctttggag tttgaacggg ttttggcgcg cccgggtcga
     2641 attcccgccc ctgctcaaca ccttgacgtg gaccccctgg gccctgcttt tcctcggcgc
     2701 caaacccggt tggaccagcg gagcgggcct ggcgctggcg atgtcttttg cgcttttggg
     2761 ggggcattgg ccccacgtgg cgtggatggg ggtggcgctt ctggcgttgt gggcggccgc
     2821 ggggggccgg cggcggggaa tcgtcctgat gtcggcgggc gtgggcgcgg cgatattctc
     2881 ggcggttcag ctgttgccgg ggttggatct gctgggacaa tcggcccgcg ttcaatcggg
     2941 cgtggcgccc gcggtggccg acctccattc cctgaccgtc gcccgttggg ccgggctcct
     3001 cgcgccggtc ttcggcgact ttccgtcgga tcgttacacg ggggagcgat ttttctggct
     3061 cggctgtttt tacatcgggg cgctggccct ttgggcggcg ggacgcgggt ggcggcgctc
     3121 ctccccccgg catcgagcga cctgggccgc gctcttcctc ctgggggggc tgttttcgct
     3181 cgggaacgcc gtcccattat acgcctgggc cgtggaccgg gtgccggggc tgcggggcgt
     3241 gcggttcccg gcccaatacg cctattggat ggtggtgtcc gccctgtccc tcctggccgt
     3301 ggtgggcggg ggcgattttc gccgaccgtg gcttcgttgg ggggcggtcg gaatcgtcgc
     3361 cgccgaactg atgttttggg gcgcgggcct tcagcccacg ctgccgggat cgtttttcca
     3421 ttcccggccg tcctggatcc ggggggtcca atcaagtccg ggaaaattgt ttttatcgcc
     3481 ccgcgcggcg gacagcatgt cggcccgggg acgaacgctc gaggaaacct ggaccgccct
     3541 tcgccatcgg ttgctggatt tgggcacgct gccttaccgg gtggccaatt taaatccctc
     3601 cgggttttcc ctcaacccgg cggggaacga taaagccctc ttggcgcttt acgcccatcc
     3661 gacgcccgcg gccgcggaac ccgattttga ccgactggac gtccgttggg tggcggcgcc
     3721 cggcgcgctc gaagttccgg ggtggcgttt gcgggaggac aatccctggc ggctttacga
     3781 gcgctcccgt ccatcggggg acggcgcggt gctttccggg gcgacccgcg cggcttggtc
     3841 ccgacggatc cgggcgttgg gacgcgacga atggacgggg gccccggcgg agaacgccac
     3901 gctcgtcacg cggacccttt ggcaaaagga ttggatcgcc ggaacggccc gaggccccct
     3961 ggacgttttg gaccgggacg gccttctgtc cgttcgctgg gaccggcccc tggaccgttt
     4021 ttgggcgcgg ttcgctccct ccagcctttg gtggggaatc cttggcacct tcgccggaac
     4081 ggcgacggcc ctcgccttcg cttttcaccg ggcgcggagc gtccgttgaa gcgcgccctg
     4141 ggattgttcg ccgccctgac gggccttttt acggccccgt tgattttcgg ccgggggttt
     4201 gtggccaacg cgggagacgt gtacgactac gcggcgccct tccgcgcctg gggcaatcgc
     4261 gaactgctcg gggggcgact ccccttttgg aatccatgga ttttcggcgg cacgcccttc
     4321 ctcgcttccc cccaatccgc cctcttttat cccgggaaca cggcgatggt ttcgacgcat
     4381 ttgacggcgg gcttccgaat cctcatcgtc ctgcacctgt tcctcatggg gatcggttac
     4441 tggatgtggt tgcgctcccg cggcgagcgt tggggcggct cggccctggg cgcggtcctg
     4501 gcgacgtttt ccttttttac catcgccaag gtggccgcgg gccacgccat tcacttgacc
     4561 ggctacgcct ggacgccgta tgtttttctc ctgagcgaag ccgtggcgcg ccggccggac
     4621 aatcgccacc ttccctgggc ggccgcggcc gcttccggac tccagtttct gacgggccac
     4681 cttcaggtct ggctcgtgac ccatctcgtc gtggcgggtc tcctggcggg ccggcgcctg
     4741 gcggaccgcc gcgtcgcgcg ggccctgggg gcggctcttc ttttcggtgc cttcctggtg
     4801 gccgtccagg cttttcccac ggccgatttt cttccccgca ccacccgggg gctgcgcggc
     4861 gtattaacgg accgggaccg ctacgccttt gccacctctt attccctgga actgggacgg
     4921 ctttggaaag ggtggaccaa cccctgggcc tggggcgccc tgccgaaacc gcctcttttt
     4981 cccgaaccgc cctcggtgta tttcgaaacg gcgacggttt tcgtgggctg gggagggctg
     5041 gccctcgcgg tcgtcggttt ggttctcctc cttcggcgcc gccaatggcg ctggccggtc
     5101 ttggcggcgg gttcgttgat ggcggctctc ggcaagaacg gccccgtcta tcccgtcctt
     5161 tggcgctgct tggcttcctt gcgcgttccg gcccggtttt atttggggat tcacgtggcc
     5221 ttggccgcgg cggccgcccg cgggtggtcc gtcctatcg
//
LOCUS       DAOQLS010000049         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7153_length_5253_cov_4.080800, whole genome shotgun sequence.
ACCESSION   DAOQLS010000049 DAOQLS010000000
VERSION     DAOQLS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7153_length_5253_cov_4.080800"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..378)
                     /locus_tag="PK362_01545"
     CDS             complement(13..378)
                     /locus_tag="PK362_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73558.1"
                     /translation="MIRRVEHIALALGAALMVGGLAARVFRGEDVDLNNAALYLPVGL
                     VALGLVNAGLRSKVLAWAIFTAALVLGMIVFGAWAVTFRSVVGSQTLNFMAATILYAA
                     LTTAAACQLRVRDHQRPGA"
     gene            complement(375..1436)
                     /gene="thrC"
                     /locus_tag="PK362_01550"
     CDS             complement(375..1436)
                     /gene="thrC"
                     /locus_tag="PK362_01550"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC73559.1"
                     /translation="MPRQGLIDKYRAHLPVSAQTPVVSLMEGETPLIPAVRIAEWAGA
                     PKLKIFLKHEGLNPTGSFKDRGMTLAISKAVEEGSKAVITASTGNTSAAAAAYAARAG
                     LRCVVLIPDGNIALGKLSQALVHGAEVFAVKGNFDDALTLVRQVAQTLPLTIVNSINP
                     YRLEGQKTGAFEVIEQLGEAPDFHFIPVGNAGNITAYWRGYRESRAAGRSAKLPRMMG
                     WQAAGSAPIVLGAPVKDPQTIATAIRIGNPASWKGALAAREESGGFIGAVTDEEILEA
                     YRALAGEGVYCEPASAASVAGVKKFARENPEFARAAGTVVCVLTGHGLKDPDRALAVS
                     PRPKSIPANLDALRAELKS"
     gene            complement(1438..2766)
                     /locus_tag="PK362_01555"
     CDS             complement(1438..2766)
                     /locus_tag="PK362_01555"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNC73560.1"
                     /translation="MRKSLVTIGLVGQGVVGSGLLKVFEENLPVLEAKVGTRLRVGWV
                     ASRRRRTLPVIGGVRPRFTKDWRDVVSDPAVDIVVELIGGLEPARTLILASLKAGKQV
                     ATANKAVLAGHWPEIFTTAQSARGLVYFEAAVGGGIPVIQGLNEGLAANRISRIFGIL
                     NGTTNYILTRMNEEGLRFNAALKAAQAAGFAEADPSFDIEGVDAAQKLAILASLATGG
                     WVKTDDVSREGLAGLELWDLNFARRRLGLVGKFLALADIDGDKVLARVHTTLIPENHP
                     FATVRHEYNAFVIHGDAVGDVMFYGKGAGAMPTASAVVSDVMYLARQVAGGMAGRMPY
                     TTYDAGRKLKIMDLEDHQMRRYLRFSAADRPGVLAAVTQILSKHEISIASVHQEDGPV
                     ALADVVRKSVPIVIVTHEAPEGAVQAALKETRRVRGLARRPVHLRIDPLR"
     gene            complement(2763..3989)
                     /locus_tag="PK362_01560"
     CDS             complement(2763..3989)
                     /locus_tag="PK362_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC73561.1"
                     /translation="MVECAKKLQQLPPYIFTRIKALAAEAHARRLDVIDLGMGNPDLP
                     TPAHVVDRLVDTVKNHPRTHRYPQAKGMPKFRRSVVRYMDRRFGVKLDPEQNVLALVG
                     SKEGIAHLCAAYLDPGDYALVPVPSYPVHSNGVILAGGRIHYMNLTPENGYLPDYGKI
                     PASVLKRTKIMFLCYPNNPTAAVVEDPNFFKETVRFAKKHDILVAYDNPYCEITFDGY
                     RAPSFLETPGARDVALEFHSFSKTYNMAGWRIGWVCGEKELLAPLEKFKAFVDYGVPS
                     FLQLAGVAALDGPQDCVAQMVETYRRRRDYFVGELNKIGWAVPLPKATMYVWAPLPES
                     WKARGSLAFAEELVKETGIVVTPGVGFGPEAEGYVRMSLVTHDDRFYDAALRIRKFLR
                     RGEKAVRPAREGKARP"
     gene            complement(3982..4281)
                     /locus_tag="PK362_01565"
     CDS             complement(3982..4281)
                     /locus_tag="PK362_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73562.1"
                     /translation="MNQKVAVRGLAHLSAWLFAGWGALMVLKGLWDCFGGEPEANHFS
                     PAPWQFVTREQWFRYAGFELCYGLACLGMAAALRVYGRRLPVFVERPLQREESHG"
     gene            complement(4295..4579)
                     /locus_tag="PK362_01570"
                     /pseudo
     CDS             complement(4295..4579)
                     /locus_tag="PK362_01570"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(4866..>5253)
                     /locus_tag="PK362_01575"
     CDS             complement(4866..>5253)
                     /locus_tag="PK362_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009634532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC73563.1"
                     /translation="KLIKDLGADVVVSDGEGLSRAGGADVILATGNSWKAAQDAMKGL
                     RPDGRLVLMGLSDETFSLTAGALMNRHRIIGSLQNDVEYLYEALDFVARGKVKVVTEI
                     YPLKDIARAYDRVSRGEVRFRAVIVP"
BASE COUNT          930 a         1672 c         1712 g          939 t
ORIGIN      
        1 ttcgatttca tcttaagccc ccggacgttg atggtcgcgc acgcgcagct ggcaggccgc
       61 cgcggtggtg agcgcggcgt agagaatcgt cgccgccata aaattgagtg tttgggaacc
      121 caccacggag cggaaggtca ccgcccaggc cccgaacacg atcatgccca gcaccagggc
      181 ggcggtgaaa atggcccagg ccaggacttt ggaccgaagc ccggcgttca ccagacccag
      241 ggcgaccagg cccaccggca gatagagcgc ggcgttgttc aaatcgacgt cttccccccg
      301 gaagacccgg gcggccaaac cgcccaccat caaggccgcc ccgagcgcca gggcgatatg
      361 ctccacccgg cggatcacga tttcaactcg gcccgcaggg catcgaggtt cgcgggaatg
      421 gatttgggcc ggggcgaaac ggccagggcc cggtcggggt ccttcaagcc gtggccggtc
      481 agcacgcaga cgacggtccc cgcggcccgg gcgaattccg ggttttcccg ggcgaatttt
      541 ttgacgccgg ccacggacgc cgcgctggcc ggctcgcaat aaacgccttc gcccgccagg
      601 gcgcggtagg cctccaaaat ttcctcgtcg gtgacggcgc cgatgaatcc gccggattcc
      661 tcccgggccg ccagggcgcc cttccaagac gcgggattgc cgattcgaat ggccgtggcg
      721 atggtctggg ggtctttgac gggggcgccc aacacgatgg gggcggaccc cgcggcctgc
      781 caccccatca tgcgggggag cttcgcgctc cgtcccgccg cgcggctttc ccggtagccg
      841 cgccaatagg ccgtgatgtt gccggcgttg cccacgggaa tgaagtggaa atccggcgcc
      901 tcgcccagtt gctcgatcac ttcaaacgcc ccggttttct ggccctccaa ccgatagggg
      961 ttgatggagt tgacgatggt gaggggcagg gtctgggcca cctggcggac cagggtcagg
     1021 gcgtcgtcaa aattgccctt caccgcgaag acctcggcgc cgtgaaccaa cgcctgggac
     1081 agcttgccca gggcgatgtt cccgtcgggg atcaacacca cgcagcggag gcccgcccgg
     1141 gccgcgtagg cggcggccgc ggccgaggtg ttccccgtgg aggccgtgat gacggctttg
     1201 gaaccctctt ccaccgcctt ggaaatcgcc agggtcatcc cccggtcttt gaaggagccg
     1261 gtggggttca gcccctcgtg tttcaggaaa attttaagct tcggcgcgcc cgcccactcg
     1321 gcaatccgga cggcggggat gaggggcgtt tccccctcca tcagggaaac gaccggggtt
     1381 tgggccgata cgggcaggtg cgcccggtat ttgtcgatga gtccttggcg cggcatgtca
     1441 gcgaaggggg tcgattcgga gatggacggg acgccgggcc aatccccgga cccggcgggt
     1501 ttccttcagg gccgcctgga cggcgccctc gggcgcctcg tgggtgacga tgacgatggg
     1561 aacggatttg cgcaccacgt cggccagagc cacgggcccg tcctcctggt ggacggaggc
     1621 gatggagatt tcgtgtttgc tcaaaatctg ggtgacggcg gccagaacgc ccggacggtc
     1681 cgcggcgctg aaacgaaggt agcggcgcat ttggtggtct tccaaatcca tgattttcaa
     1741 tttacggccg gcgtcgtagg tggtgtaggg catgcggccg gccatgccgc ccgccacctg
     1801 gcgcgccaga tacatgacgt ccgacaccac ggcactggcc gtcggcatgg cgcccgcccc
     1861 tttgccgtaa aacatcacgt cgcccacggc gtcgccgtgg atgacgaagg cgttgtattc
     1921 gtggcgcacc gtcgcgaaag ggtggttttc gggaatgagg gtcgtgtgga cccgggccag
     1981 cactttgtcg ccgtcgatgt cggccagggc caaaaacttg ccgaccaggc ccaggcgccg
     2041 ccgggcaaaa ttcaaatccc agagttccaa ccccgccaac ccctcccggg acacgtcgtc
     2101 ggttttgacc cacccgcccg tggccaggga ggccaggatg gccaatttct gggccgcgtc
     2161 cacgccttcg atgtcgaagg aggggtccgc ttcggcgaaa cccgcggctt gggcggcttt
     2221 gagggcggcg ttgaaccgga gcccttcctc gttcatccgg gtcaatatat agttcgtcgt
     2281 gccgttcaag atgccgaaaa ttcgggaaat ccggttggcc gccaggccct cgttcaggcc
     2341 ctgaatgacg ggaatgccgc cgcccaccgc ggcttcaaag tatacgaggc cacgggccga
     2401 ctgggccgtg gtgaaaattt ccggccagtg gccggcgagc acggccttgt tggccgtggc
     2461 gacctgcttg cccgccttga gcgacgccag gatcaaggtt cgggccggct ccaacccgcc
     2521 gatgagctcc accacgatgt ccacggcggg gtccgacacc acgtcccgcc aatccttggt
     2581 gaaccggggg cggacgcccc cgatcacggg caacgtgcgc cgtcgacggc tggccaccca
     2641 ccccacgcgg aggcgcgtgc ccaccttggc ctccaggacg ggcagatttt cctcaaaaac
     2701 cttcagcagg cccgagccga ccacgccctg gcccacgagg ccgatggtga cgaggctttt
     2761 tctcaaggcc gggctttgcc ttcccgggcg ggacggacgg ctttttcccc ccgtcgaaga
     2821 aatttgcgga tgcggagggc cgcgtcgtaa aaacgatcgt cgtgggtgac cagggacatg
     2881 cggacgtagc cctccgcctc cgggccgaac ccgaccccgg gagtcaccac gatgccggtc
     2941 tccttgacga gctcctcggc aaaggccagg gaaccccggg ccttccagga ttcgggcagg
     3001 ggcgcccaga cgtacatggt cgccttgggc aggggcacgg cccagccgat cttgttcagt
     3061 tccccgacga aatagtcccg gcgccgacgg taggtttcca ccatctgggc cacgcagtcc
     3121 tggggcccgt ccagggcggc gacccccgcc aactgcagga agctcggaac gccgtagtcc
     3181 acaaaggctt tgaatttttc gagcggagcg agcagttcct tttccccgca cacccagccg
     3241 atgcgccagc cggccatgtt gtaggttttg gagaaactgt gaaattccag cgccacgtcg
     3301 cgggcccccg gcgtttcaag aaaactgggc gcacggtaac cgtcgaacgt gatctcgcag
     3361 taggggttgt cgtaggccac caggatgtcg tgttttttgg cgaagcgcac ggtttccttg
     3421 aaaaagttcg ggtcctccac caccgccgcc gtggggttgt tggggtagca gaggaacatg
     3481 atcttcgtgc gctttaagac cgacgcgggg attttcccgt aatccggcag atagccgttt
     3541 tccggcgtga gattcatgta gtgaatccgt ccgccggcca aaatgacgcc gttggaatgg
     3601 acggggtagc tgggcacggg gaccagggcg taatcccccg ggtccaaata ggcggcgcac
     3661 aggtgcgcga tgccttcctt ggaacccacc agggccaaaa cgttttgttc cggatcgagc
     3721 ttgacgccaa agcggcggtc catgtagcgg acgaccgacc gccggaattt gggcatgcct
     3781 ttcgcctggg ggtagcggtg ggtgcggggg tgatttttga ccgtgtccac caggcggtcc
     3841 accacgtggg ccggcgtcgg cagatcgggg ttgcccatgc ccaggtcgat cacgtccagc
     3901 cggcgcgcgt gggcctcggc ggccagggcc ttgatccggg tgaaaatgta ggggggcagc
     3961 tgttgaagtt ttttggcgca ctcaaccatg ggattcctct cgttgaaggg gacgttcgac
     4021 gaacaccggc aagcggcggc cgtacacgcg cagggcggcg gccattccca ggcacgccaa
     4081 tccgtaacac aactcaaacc ccgcgtagcg gaaccactgc tcccgggtga cgaattgcca
     4141 gggggccggg gaaaaatggt tggcctcggg ctcgcccccg aagcagtccc acaacccttt
     4201 caagaccatc agggcgcccc aaccggcgaa caaccacgcc gacaaatggg ccaagccgcg
     4261 caccgcgact ttttgattca tgccggcggg ccgcttaacc tttgacgagt ttcttgaggg
     4321 aaaggctggg tttgaaggaa atttttcgcc cggcgggaat ggcgatggtc tcgccggtct
     4381 tggggtttcg gcccgggcgg gctttccggg ctttcacttt gaaggtgccg aggccggtca
     4441 gggaaatttt gtcgccggaa cgaatcgtgt cccggatggc gctcaccagg gatttgacgg
     4501 atttgatcgc ctgggcgcgg gtgacgccgg cgtccttggc cacgcgctga atgagttcaa
     4561 acttgttcat gtttcctccg gaaaatgggc gatcaacgag ttaaatcgac tctattttta
     4621 gataagaaaa gtctcttgtc aagggatggc ctcgcccctt cgggctcgtc caccattcgc
     4681 accgggcttt gtcccgcgcg aacacgggag ggtctttaca tccgggcggt ccctccgcgt
     4741 caagggatgg cctcgcccct tcgggctcgt ccaccattcg caccgggctt tgtcccgtgc
     4801 gaatacggga gggtctttac atccgggcgg tccctccgcg tcaagaacaa ttcgacggga
     4861 atcgatcagg gaacgatgac cgcccgaaag cggacttccc cccgcgacac ccggtcgtag
     4921 gcccgggcga tgtctttcag gggataaatt tccgtcacga ctttcacctt cccccgggcc
     4981 acgaaatcca acgcctcgta aaggtattcc acgtcgttct gtagggaacc gatgatccgg
     5041 tggcgattca tcagggcgcc ggccgtgagg gaaaacgttt cgtcggagag gcccatgagc
     5101 accagacggc cgtccggacg aagtcccttc atggcgtcct gggcggcctt ccaggaattg
     5161 cccgtggcca aaatcacgtc ggcgcccccg gcccgggaaa gaccctcccc gtccgacacg
     5221 acgacatcgg ctcccaagtc tttgataagt ttc
//
LOCUS       DAOQLS010000050         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7154_length_5253_cov_3.907849, whole genome shotgun sequence.
ACCESSION   DAOQLS010000050 DAOQLS010000000
VERSION     DAOQLS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7154_length_5253_cov_3.907849"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..949)
                     /locus_tag="PK362_01580"
     CDS             complement(197..949)
                     /locus_tag="PK362_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC73564.1"
                     /translation="MGRNQTARKSPKKALNPRPVRAVFFDAGNTLLRAHPSVGHIYTR
                     AARRHGLRLPVAWVEERFQAAWRDRHRHALPKNDNAEKLWWRKMVRRVFGARFAPRAF
                     DTFFNDLYGRFAHPRHWRLFDDALPTLRALRRRGFRLGIVSNWDSRLVTLSEKIGLTR
                     EVEFLMVSSLEGVAKPDPTIFERALARAGVAPHEALHVGDSLREDYLGARAAGLSALL
                     LERHGTGPAGISTIQSLEGVLRHAGPRPAPSR"
     gene            complement(904..1293)
                     /locus_tag="PK362_01585"
     CDS             complement(904..1293)
                     /locus_tag="PK362_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HNC73565.1"
                     /translation="MAKQNLNDLLRLMARLRAPGGCPWDRRQTHKSLVRHLKEESAEV
                     ALAIRKNDPDNLCEELGDLLHQIVFHAQLAREKKRFTMADVIDGLCRKIVRRHPHVFG
                     KKKLKSVKEVMVQWEEIKRREKARKKR"
     gene            1460..2098
                     /locus_tag="PK362_01590"
     CDS             1460..2098
                     /locus_tag="PK362_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNC73566.1"
                     /translation="MVFFPAFLELSGKECLVVGGGDVALQKTRDLIAARARVTVVATR
                     FCPEFRKHKGLRRLRRAFRPGDVPAGPGRPWLVVAATDDEALHARIADLCRRRRVWVN
                     VVDRPAFCDFIVPSVARRGLVTVAVSTGGQSPALAKFIGGKIRASLGPEVGRLGRLLA
                     DLRPALRRLPMAERRELLSCFVSDGALAEIRRSGAVALRRYKKMILNSVPRE"
     gene            2102..3022
                     /gene="ccsA"
                     /locus_tag="PK362_01595"
     CDS             2102..3022
                     /gene="ccsA"
                     /locus_tag="PK362_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNC73567.1"
                     /translation="MQRWEALLFDFGYLGYAAALVLAVVYALTRRDDLMALGHRLLVG
                     AALLHSASLAVGLWAESHRPGHVGYAFWSQWFASLSLFALLIVLIYLFAQHRAPVSIL
                     GVFVLPLAVFLLTLALTQAFLASPRCPFASVEDFLKIADATRRQPDIAPTFWTAVHVP
                     LIFLAYAAFANAFGIGLAYLIGERQIKSHRPTELGYRLPALEVMDRLIAHIVLIGFPA
                     LTVGLALGAGGARAAGGPGWAGDPKVLWSLVMWGVYLSYIVLRYVVGWRGRRTAYLSL
                     AGFGVVLFTYVGVSFFSRMHGFLQAGGALP"
     gene            3019..4131
                     /gene="hemA"
                     /locus_tag="PK362_01600"
     CDS             3019..4131
                     /gene="hemA"
                     /locus_tag="PK362_01600"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNC73568.1"
                     /translation="MKLRLVGLSHRTAPVEWRERLAVPEGRLPVLVESIKNSAGAVET
                     VVLSTCNRVEVYAVTPDDGETECLLRKELSGLHLDKTLDDSLYSLEDDAAVRHLFRVA
                     AGLDSLVIGESEILGQVKRAYDLAQRAGSTGKLTNVLFQRAIYLGKKVRTATKISEGP
                     TSVPSLAVALAERIFGDLSTSRVFVLGAGPMAEMAVRALKSQKVADLLVANRTGEKAE
                     AMARQFGGRAIPFDRRAEGLVEADIVLCSTGAPDVVITRNDVARALERRRGKSLFLID
                     IAVPRDVDASVNDLENTYLYNVDDLESLVAESLVKRRDEIQKADGLVDDKTNEFAAWY
                     RAWLAGDTATLRHAPAESADGSAAAETKGGIVSWPN"
     gene            4119..5039
                     /gene="hemC"
                     /locus_tag="PK362_01605"
     CDS             4119..5039
                     /gene="hemC"
                     /locus_tag="PK362_01605"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNC73569.1"
                     /translation="MAQLIIGTRGSALALAQSKMVKARLEALAPDLRVDLQIIKTTGD
                     KMTESPLANLGGKGVFTKEIEEALLDRRVDLAVHSLKDLPTQLPPGLTVGAVLEREDP
                     RDCLVSRFGEQLLELPSGSVVGTSSLRRQAQIRAAKKNIRVQDTRGNLDTRLKKVAEG
                     EFTAVVVAYAGVRRLGRADEVSEVIPLEIMLPAPGQGFIAIEIREGDEATAVWVKRLN
                     HEMSFRSATAERAFLAGLGGGCRVPIAAHARADGNRLVMDGLVISVDGQRLLRVKDQG
                     PLTDPLGLGNLLAARALEKGAANILKETVS"
     gene            5050..>5253
                     /locus_tag="PK362_01610"
     CDS             5050..>5253
                     /locus_tag="PK362_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HNC73570.1"
                     /translation="MAESIHGHEVMRLMAAAPAGIPRDQLERAIAERFGPEARFHTCS
                     ASNLTAGDLIRFLEGRGKFSPTTD"
BASE COUNT          850 a         1639 c         1754 g         1010 t
ORIGIN      
        1 cggaccaacg ggaggtcgtt aaaaaaaacc gtcccgcggt gttggacgat tcccaccaat
       61 tcgaaacggg gcaacgcgga aaaggagacc tcaacgcttt tcattcgaga ttcgacgggt
      121 cgcggtccca gaaccagcgc gaccccggcc aaaatccgtc caagggacgc ggtcggtcga
      181 aggcgaaaac gcgggttcaa cgggagggcg cgggccgggg tccggcatgg cggagaacgc
      241 cctccaacga ttgaatcgtc gatatccccg cgggtcccgt cccgtggcgt tccagaagaa
      301 gggccgacag cccggcggcc cgggcgccga ggtagtcttc ccggagggaa tcccccacgt
      361 gcagggcctc gtggggggcg acgcccgccc gggccagggc ccgctcgaaa atggtggggt
      421 ccggcttggc cacgccctcc agggacgaca ccatcaaaaa ctccacttcc cgggtcaggc
      481 cgattttttc ggacaaggtc accaaccggg agtcccaatt ggaaacgatc cccaggcgaa
      541 acccccggcg tcgaagggcg cgcagggtcg gtagggcgtc gtcgaacaaa cgccaatggc
      601 gggggtgggc gaagcggccg tagagatcgt tgaaaaacgt gtcgaaggcc cggggcgcga
      661 accgggcgcc gaagacccga cggaccattt tccgccacca gagtttctcg gcgttgtcgt
      721 ttttgggcag ggcgtggcgg tggcggtccc gccaggcggc ttgaaaccgt tcctccaccc
      781 aggccacggg caagcgcagg ccgtgccggc gggcggcccg ggtatagatg tgccccacgg
      841 atgggtgggc gcgcagaagg gtgtttcccg cgtcgaagaa gaccgcgcgg accgggcggg
      901 ggttcagcgc ttttttcggg ctttttcgcg ccgtttgatt tcttcccatt ggaccatgac
      961 ttctttgacg cttttgagtt tcttctttcc gaaaacgtgg gggtgccggc ggacgatttt
     1021 gcggcagagg ccgtcgatca cgtccgccat ggtgaacctt tttttctccc gggccagttg
     1081 agcgtgaaaa acgatttggt ggagcaaatc gcccagttcc tcgcagaggt tgtccgggtc
     1141 gtttttgcgg atggccagcg ccacttcggc ggattcttcc tttaaatggc gaaccaaact
     1201 tttgtgggtt tgacgccggt cccagggaca accgcccggg gcccgaagcc gggccatcaa
     1261 gcgcaaaagg tcgtttaagt tttgtttcgc catgggagcc taattctata tatttccgtc
     1321 gggtattggg gttttccccg ggggtttttt tggtattatg gcccttcatg gaatccggcc
     1381 ccgcggtcct ttcccagagc tccaaggacg gggcgcgttg cgaaggcgcc ggacgcggaa
     1441 tgggtcggta atcgaattca tggttttttt tcccgctttt ctcgaattgt ccggcaagga
     1501 gtgcctggtg gtcggcggcg gcgacgtggc ccttcaaaaa acccgggatt tgatcgccgc
     1561 ccgggcccgg gtcacggtgg tggccacccg gttttgcccc gaatttcgga aacacaaggg
     1621 tcttcggcgg cttcggaggg cctttcgccc cggcgacgtt cccgcgggtc ccggccgccc
     1681 ctggttggtg gtggcggcca cggacgatga ggcccttcac gcccggatcg ccgatctttg
     1741 ccgtcgtcgg cgggtctggg tcaacgtcgt cgatcggccc gccttttgcg atttcatcgt
     1801 cccgtccgtg gcccggcgcg ggcttgtgac ggtggcggtt tccaccgggg gccaaagccc
     1861 ggccctggcc aaattcatcg gcggaaaaat ccgggcctcg ttgggtcccg aggtcgggcg
     1921 tttgggacgc ctcctggcgg acctgcggcc cgcccttcgg cgactgccca tggcggagcg
     1981 ccgggaactg ttgtcttgtt tcgtttcgga cggcgccctg gcggaaatcc gccgctccgg
     2041 cgccgtcgcc cttcggcggt ataaaaaaat gattttgaat tccgttcctc gggagtaacg
     2101 cgtgcaacgt tgggaagccc tgttgttcga tttcggttat ttggggtacg ccgcggccct
     2161 ggtgttggcc gtcgtctacg ctctgacccg tcgggacgat ttgatggccc tgggccaccg
     2221 cctgctggtc ggggcggccc tcctccattc cgcgagcctg gccgtgggcc tctgggcgga
     2281 atcccaccgg cccggacacg tcggttacgc tttttggagc caatggttcg ccagtctctc
     2341 cctcttcgct cttctgatcg ttctgattta tctcttcgcc caacaccggg cgcccgtgtc
     2401 catcttgggg gtgttcgtgc tgcccctggc cgttttcctc ctgaccttgg ccctcaccca
     2461 ggcgttcctg gccagtcccc gttgcccctt cgcctccgtg gaggattttc tgaagatcgc
     2521 cgacgccacg cgccgtcagc ccgacatcgc ccccaccttt tggaccgccg tgcacgtgcc
     2581 gctcattttc ctcgcctacg ccgcttttgc caacgccttc gggatcggcc tggcctacct
     2641 gatcggcgaa cgccagatca aatcccaccg gccgacggaa ttgggctacc gccttcccgc
     2701 gttggaggtc atggaccggt tgatcgctca catcgtgttg atcgggttcc cggcgctgac
     2761 cgtggggctc gctttgggcg ccggcggcgc ccgcgccgcc ggggggcccg gctgggccgg
     2821 agatcccaag gtcctttggt ccctggtcat gtggggcgtc tatttgtcgt acatcgttct
     2881 tcgttacgtg gtcggctggc gcggccggcg aacggcctat ttgtccctgg ccgggttcgg
     2941 ggtggtgttg ttcacctacg tgggcgtcag ctttttctcc cggatgcacg ggttccttca
     3001 ggccgggggg gcgctcccgt gaagcttcgg ctcgtcggcc tctcccaccg caccgcgccg
     3061 gtggaatggc gggaacgcct ggcggtgccg gaaggccgtc ttccggtgtt ggtggagtcg
     3121 atcaagaaca gcgcgggggc ggtggaaacg gtggtgctgt ccacctgcaa ccgggtggaa
     3181 gtctacgccg tgacgcccga cgacggcgaa acggaatgct tgttgcgaaa agaactgtcg
     3241 ggtcttcatc tcgataaaac cctggacgac agtctttaca gtttggagga cgatgcggcc
     3301 gtgcggcact tgttccgcgt ggcggcgggg ttggattccc tggtgatcgg cgaatcggag
     3361 atcctcgggc aggtcaaacg cgcctacgat ttggcccaac gggccgggtc caccgggaaa
     3421 ctgaccaacg tcctgtttca gcgggccatt tatctcggaa aaaaagtccg gaccgccacg
     3481 aaaatatccg aggggcccac ttcggtcccc agtctggcgg tggccctcgc cgagcgcatt
     3541 ttcggggatc tgtcgaccag ccgggtgttc gtcctgggcg cggggcccat ggcggagatg
     3601 gccgttcggg cccttaagtc ccagaaagtc gcggatctcc tggtggccaa ccggaccggg
     3661 gaaaaggccg aggccatggc gcggcaattc ggcggccggg ccattccctt cgaccgacgg
     3721 gcggaggggt tggtggaggc cgacatcgtc ctgtgctcca ccggggcgcc ggacgtcgtg
     3781 atcacgcgaa acgacgtggc ccgggccctg gagcggcgcc ggggaaaatc cctctttttg
     3841 atcgacatcg cggtgccccg ggacgtggac gcctcggtca acgatttgga aaacacctac
     3901 ctctacaacg tggacgattt ggaatccctg gtggccgaaa gcctggtcaa gcggcgcgac
     3961 gaaatccaaa aggccgacgg cctcgtggac gacaaaacga acgagttcgc cgcctggtac
     4021 cgggcttggc tggcgggcga caccgcgacc cttcgccacg ccccggccga atcggccgat
     4081 ggatccgcgg cggccgaaac gaaaggcggg attgtctcgt ggcccaattg atcatcggca
     4141 cccgggggag cgccctggcc ctggcccaat cgaaaatggt caaagcccgg ttggaggccc
     4201 tggcccccga tctgcgcgtc gaccttcaaa tcatcaagac gaccggggac aaaatgaccg
     4261 agtcgcccct ggcgaacctg gggggcaagg gcgttttcac caaggagatc gaagaggcgc
     4321 tcctggaccg ccgggtggac ctggcggtcc attccctgaa ggatttgccc acgcaactgc
     4381 cccccgggct cacggtgggc gccgtgcttg aacgggaaga cccccgggat tgcctcgttt
     4441 cccggttcgg cgaacagctg ctggaattgc cgtccggctc cgtggtgggc accagctccc
     4501 tgcgtcggca ggcccagatt cgcgcggcca agaaaaacat ccgggttcaa gacacccggg
     4561 ggaacctgga cacgcggctg aagaaagtgg ccgaaggcga attcaccgcc gtggtcgtcg
     4621 cctacgcggg ggtgcggcgg ctgggccggg ccgatgaagt gtccgaagtg atccccttgg
     4681 aaatcatgct cccggccccc gggcagggat tcatcgccat cgaaatccgc gaaggcgacg
     4741 aggccaccgc ggtctgggtg aaacgcctga accacgaaat gagttttcgc tcggccaccg
     4801 ccgaacgggc ctttttggcc gggctgggcg gcggttgccg ggtgccgatc gcggcccacg
     4861 cccgggccga cggcaaccgg ctggtgatgg acggtctcgt gatcagcgtc gatgggcagc
     4921 gactcctccg cgtgaaagac caggggcccc tcacggaccc cctggggttg ggaaacctgt
     4981 tggccgcccg ggccttggaa aagggcgccg cgaacatttt gaaagaaacg gtttcctgaa
     5041 aggaacgcca tggccgaatc catccacgga cacgaagtaa tgcgcttgat ggccgccgcg
     5101 ccggcgggga ttccccggga ccaattggag cgggccatcg ccgaacggtt cggccccgag
     5161 gcgcgttttc acacctgttc cgcttccaac ctgacggcgg gggatttgat ccggtttttg
     5221 gagggccgcg ggaaattctc gcccacgacg gac
//
LOCUS       DAOQLS010000051         5241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7185_length_5241_cov_4.003471, whole genome shotgun sequence.
ACCESSION   DAOQLS010000051 DAOQLS010000000
VERSION     DAOQLS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7185_length_5241_cov_4.003471"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PK362_01615"
     CDS             <1..1096
                     /locus_tag="PK362_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73571.1"
                     /translation="WGGYLSRPSGTLLSYWIDGDFFSPSSYEAVDLSALLTPDAQGFG
                     TGFLDQSHWCYFAPLRKETNGKMGPNDLALRYDRLKGMSNPAAYETFRVGSLPRAPAR
                     MGWITGAEINGYAFFVPYGEPTRGPPWFHRHGNLLRYNSRGPFADPRSWDWVDLTALV
                     HPDAVGYQSCAVKEPWIYLIPYDRNILIRYTPSKPLGSPSSYESVDLTRLHPKAVGYT
                     GGIVSGPYLVLVPYRDLSIRNLFKQSVNLAAAYDTRRPLADRSAWSFIDLSEVDKEAR
                     GGYQFGWRDNEGFVHFCPTFNFRTRKPPSFVVWDGRKSFSDPRSWRAFPSKGVPTSTG
                     AAYDGSRAYLVPWGKNGDSGRITRISMERE"
     gene            complement(1191..1673)
                     /locus_tag="PK362_01620"
     CDS             complement(1191..1673)
                     /locus_tag="PK362_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73572.1"
                     /translation="MSRRRAIDFSRNGTAGFTLIELMLVVAIIGLLAAIALPKFGEVL
                     RKAKEASVRGKLGSLRSAMELYYVDNEGALYPRWTTNQGVPLLVPKYIDAIPPIGVPH
                     WDDSPETNQLVSNIVPNCDLAHAFDDRYVYQDFISPPRYVLYVACIHTDARRGTWSQY
                     "
     gene            complement(1798..>5241)
                     /locus_tag="PK362_01625"
     CDS             complement(1798..>5241)
                     /locus_tag="PK362_01625"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNC73573.1"
                     /translation="DPATLIVPKERRFRVLEPADRHAHRVRTLPPEPPVAGSASAPGI
                     LGLDLGSTGAKAVLTSLSDGCAVWDLYDTTRGNPVEAAKRLVAAALARGPLDVRAVAV
                     TGSGREAVATVLRAAYPDAADRIVVANEIIAHATAAIRQDPHRGRSLSVVEIGGQDAK
                     FIQISGGQIVESDMNRACSAGTGSFLEEQAKCYGIDDVREFVRRAREARRPPDLGQMC
                     TVFVAEAAAEALNEGFTLSDVFGGFQYSVVHNYIHRVMSHRVFGETIFFQGKPASGPS
                     LAWTLAAVTGREVLVPDNPGAMGAWGIGLLAREERRGALEGAATLDLARFGESRIVES
                     RDFQCHEPRCATLCRIEKTTVEGPGGARTVYNGGACPKYEISTAERPKLPLEAPSAFD
                     EREALLAPFVAGAPGENPVGVPWVGALAGLLPWTVAFLKGLGLPVRVLRSDATSLARG
                     EGRCFSYDSCAPAKLAHGVVPPEVERIFLPKVIDRPADRSPGVTCAMEQALPDILRMS
                     GRSRGEEARWASPALSFARGFDAPELRGPLEEAARRLGADPALVSSALSAAARAQEEY
                     ETALAGIGRRTLEYGREHGIPVVAVVGSLHVIHDSAINAGVPRLLRTEGVLPLPMDCF
                     PLSEDEVPGLERLAWGDAKRALRVGLACRARGDAYPLLLTAFGCSPLSFGEQFNEALL
                     AGHPHTTLESDGHGGTAGYVTRVQAFLHTVRRHDRRPSPPPTDALRWTAPLPEEPLVP
                     GGPPLLVFSVADRLPGILAANYRAHGVDARPAAPSGPAALAAGRSDCTGKECLPYQML
                     WGTFRKTLEDLAPGTPARLLQFSGEGACRNCVFSAKDQMSLARNGWADRVGVRHFGPE
                     PRLGRAFFFRLYAGVVAWELLHQLASYHGARAAAPARALYDRFGEELENIMARPGASG
                     PRGWAAAGGVAVDVHALLLRASRAFAEMSGPAPLRRALLSGDVYVRLDAGANDDLVRR
                     LNRLGVAVLVEPLSSLAEYILDEKSAELFRLPTGFPERPLTRAAMVGLRRWYHAAARR
                     YNPWLPTVTPADMAEGTRGKLDRHPRGEAPVTLGSVYSHWNRGLCDGAVVASPWGCGP
                     ALVAENLLRHDKEIPLLFLYCDGSPIDDRKLNAFAFRLRRTPARSSGSKEERPGTLSR
                     G"
BASE COUNT          952 a         1792 c         1662 g          835 t
ORIGIN      
        1 gtggggaggg tatttgagcc gccccagcgg aacgctcttg agttattgga tcgatgggga
       61 ctttttttcc ccctcgagtt atgaagcggt ggatttgtcc gctttgctca cccccgacgc
      121 ccaagggttc ggaaccgggt tcctggatca atcccattgg tgttacttcg ccccccttcg
      181 aaaagagacc aacgggaaaa tgggccccaa cgacttggcc cttcgctacg accgcctcaa
      241 gggaatgtcc aaccccgccg cctacgagac gtttcgcgtc gggtccctcc cccgtgcgcc
      301 ggcgcgaatg gggtggatca ccggggcgga aataaacggt tacgcctttt ttgtgcctta
      361 cggcgagccg acccggggcc ccccatggtt ccatcgccat ggaaatctcc tccggtacaa
      421 cagccgaggc ccctttgccg acccccgttc gtgggattgg gtcgacctga ccgccctcgt
      481 ccacccggac gctgtcggat accaatcctg cgcggtcaaa gaaccgtgga tctacctgat
      541 tccttacgac cgaaatattt tgatccgcta caccccttcc aagcctttgg gatccccctc
      601 ttcctacgaa agcgtggacc tgacccgcct tcatccaaaa gccgtgggtt acaccggggg
      661 aatcgtttcc ggtccctacc tggtcctggt tccctatcgg gacctatcga ttcgcaacct
      721 ttttaaacaa agcgtcaatc tcgcggccgc ctacgacacg cgccgtccct tggccgatcg
      781 atccgcctgg tcctttatcg atctctccga ggtggacaaa gaggcccgcg gcggatacca
      841 attcggttgg cgggacaacg aagggtttgt ccatttctgc cccactttca acttcaggac
      901 gaggaaaccc ccgtccttcg tggtctggga cggtcggaaa tccttttccg atccccgatc
      961 ctggagggcc tttcccagca aaggcgttcc gacatcgacc ggggccgcct acgacggaag
     1021 ccgcgcctac ctggtcccct ggggaaaaaa cggcgacagc ggaaggatca cgcggatttc
     1081 catggagcgg gaataacgcg ggcgaccgtc atcgccccga cgcccccgcg ggacttcgtc
     1141 ccactggctt ggaatgcgct tcgccccctc ccggcccccc caaagacctt tcagtattgg
     1201 ctccacgttc ctcggcgggc gtccgtgtga atgcacgcca cgtacaagac atacctcggc
     1261 ggcgatataa aatcctggta aacgtaacga tcgtcgaagg cgtgggccaa atcacagttg
     1321 gggacgatat tgctgaccaa ttgattcgtt tcgggcgagt cgtcccagtg gggaacgccg
     1381 atcggcggaa tcgcgtcgat gtattttggg accagaagcg gcacgccctg attggtggtc
     1441 caccgcggat aaagagcgcc ttcgttgtcc acgtaataca actccatcgc cgaccgaaga
     1501 ctgcccaact ttccccggac ggaagcttcc ttggccttgc gaagcacttc cccgaatttg
     1561 ggaagggcga tggcggccaa aaggccgatg atggcgacca caagcatcag ctcgatgagc
     1621 gtaaatccgg cggttccgtt ccgcgaaaaa tcaatggccc ttcgtcggct cacgtcacac
     1681 cccgtttcga agggctccaa acccttcctt cgctcccgcg ttaaagggca atccgacttt
     1741 cccacggtcc agccgcttct cccgatcggt tccccgatgt ctttcgaacc actggagtca
     1801 gccccgggaa agagtccccg gcctttcctc ctttgatccc gaagagcggg ccggcgtccg
     1861 ccggaggcgg aaggcgaaag cgttcaattt tcggtcatcg atgggcgacc cgtcgcagta
     1921 aaggaacagg agcgggatct ccttatcgtg ccgtagaaga ttttcggcga ccaacgccgg
     1981 tccgcacccc caggggctgg ccaccacggc gccgtcgcac agaccccggt tccaatggga
     2041 atacacgctt cccagcgtca ccggcgcttc cccgcgcggg tggcggtcca gtttgccccg
     2101 ggtgccctcc gccatgtccg ccggagtcac cgtgggcaac caagggttgt accgccgggc
     2161 cgccgcgtga taccatcggc ggagtcccac catcgcggcc cgggtgaggg gccgttccgg
     2221 aaagcccgtc ggcagtcgga aaagctcggc ggacttttca tccaggatgt attccgccag
     2281 ggacgacagc ggttccacca aaacggccac ccccaggcgg ttgaggcgtc gaaccaaatc
     2341 gtcgttggcc ccggcgtcca gacggacgta gacgtcgccg gacaacagcg cccgccgaag
     2401 gggcgccggt ccggacattt cggcgaaggc ccgggaggcc cggagcagca gcgcgtggac
     2461 gtcgacggcg acgccccccg ccgcggccca tccccgcggg cccgatgctc cgggacgggc
     2521 catgatgttt tccaattcct ccccaaaacg atcgtagagc gcccgggccg gggccgccgc
     2581 ccgggcccca tggtaagacg ccagttgatg cagcaattcc caggcgacga cgcccgcgta
     2641 caaccggaaa aagaacgccc gacccaaacg gggctccggt ccgaagtggc ggacgcccac
     2701 ccggtcggcc catccgttgc gggccaggga catctgatcc ttggcggaaa aaacgcaatt
     2761 tcgacaggcg ccctcaccgg aaaattgcaa aaggcgggcg ggggttcccg gggccaggtc
     2821 ctccagggtt ttccgaaagg tcccccaaag catttgatag gggaggcatt ccttgccggt
     2881 gcaatcgctc cgccccgccg ccagggccgc ggggcccgag ggcgcggccg gccgcgcgtc
     2941 cacgccgtgg gcgcgataat tcgccgccag gattccgggc agacgatcgg ccacggaaaa
     3001 aacgaggagc ggcggtccgc ccgggacgag gggctcttcg ggaagcggcg cggtccaacg
     3061 cagggcgtcc gtcggcgggg gcgagggtcg ccggtcgtgc cggcgcaccg tgtgcaaaaa
     3121 ggcctgaacc cgggtgacgt atcccgcggt ccctccatgc ccatcgcttt ccaaggtggt
     3181 gtgggggtga ccggccagga gcgcttcgtt gaactgctcg ccaaaggaaa gggggctgca
     3241 gccgaaggcc gtgaggagga gtgggtacgc gtcgccccgg gcgcggcagg ccaatccgac
     3301 ccgcagggcc cgtttggcgt ccccccaggc caggcgttcc aaaccgggca cttcgtcttc
     3361 ggacaaggga aaacaatcca tgggcaaagg aaggacgcct tcggtccgca gcaaccgagg
     3421 cacgcccgcg ttgatggcgg aatcgtgaat cacgtgcagg gaacccacga cggccacgac
     3481 ggggatcccg tgctcccggc cgtattccag ggtccgacga ccgatgcccg ccagggccgt
     3541 ctcgtactcc tcctgggccc gggcggcggc cgacagggcc gacgaaacga gggccggatc
     3601 ggcgcccaaa cggcgggcgg cctcttccag aggccctcga agctccggcg cgtcgaaccc
     3661 acgggcgaag gacagggcgg gggaagccca acgggcctct tcgccccggg accgtcccga
     3721 cattcgaagg atgtccggca gggcctgctc catggcgcag gtgacgcccg gcgaacgatc
     3781 ggcgggccgg tcgatcacct tgggaaggaa aatccgctcc acctcgggcg gaaccacgcc
     3841 gtgggccagt ttcgcgggcg cgcaggaatc gtaggaaaaa caacgcccct cgccgcgggc
     3901 cagggacgtg gcgtcggagc gaagaacgcg caccggaagc cccagccctt ttaaaaaggc
     3961 gaccgtccag ggcaacaacc ccgccagggc gccgacccag ggaacgccga cgggattttc
     4021 cccgggcgcg cccgcgacga agggggccag gagcgcctcc cgctcatcga aggcgctggg
     4081 ggcctccagg ggcagcttcg gccgttccgc cgtggaaatt tcgtatttcg ggcaggcgcc
     4141 tccgttgtaa accgtccggg cgcccccggg cccctcgacg gtggttttct cgatgcggca
     4201 gagggtcgcg caccggggtt cgtggcactg gaaatcccgg gactccacga tccgggattc
     4261 cccgaaacgg gccagatcca aggtcgccgc gccctccagg gccccgcgac gctcctcccg
     4321 cgccaggagg ccgatccccc aggcccccat ggcccccggg ttatcgggca ccagaacctc
     4381 ccgcccggtc acggcggcga gggtccaggc cagggacggc cccgaggccg gtttgccctg
     4441 gaaaaagatg gtctcgccga aaacgcggtg ggacatcacc cggtggatgt aattgtgcac
     4501 gacggagtac tggaaccccc cgaacacgtc ggacagggtg aacccttcgt tcagcgcctc
     4561 ggccgcggcc tcggccacga acaccgtgca catctggccc aaatcgggcg gacggcgcgc
     4621 ctcccgggcc cggcggacga actcccgcac gtcgtcgatg ccgtagcact tggcctgttc
     4681 ctccaaaaac gatcccgtcc cggcgctgca ggcccggttc atgtcgcttt cgacgatctg
     4741 ccccccggaa atctggataa atttggcgtc ctgcccgccg atttcaacga cggaaagact
     4801 tcggccccgg tgggggtcct ggcggatggc ggccgtggcg tgggcgatga tctcgttggc
     4861 cacgacgatc cggtccgcgg cgtcggggta ggccgcccga agaaccgtcg ccacggcttc
     4921 ccggccggaa ccggtgacgg ccacggcgcg aacgtcgagc ggtccccggg ccaaggccgc
     4981 ggccaccagc cgcttggcgg cctccaccgg attgccccgg gtcgtgtcgt ataaatccca
     5041 caccgcgcaa ccgtcggaca acgacgtcaa caccgccttg gctcccgtcg atcccaaatc
     5101 caaacccaaa atcccggggg ccgacgcgga cccggccacg ggcggttccg ggggaagcgt
     5161 gcgcacgcgg tgggcgtggc ggtcggccgg ttccaacacg cggaaacggc gttccttggg
     5221 gacgatcaac gtcgcggggt c
//
LOCUS       DAOQLS010000052         5215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7243_length_5215_cov_4.961240, whole genome shotgun sequence.
ACCESSION   DAOQLS010000052 DAOQLS010000000
VERSION     DAOQLS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7243_length_5215_cov_4.961240"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PK362_01630"
     CDS             <1..558
                     /locus_tag="PK362_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC73574.1"
                     /translation="LEAMGQHFLSRFLSERAPALQPAPRGVECRFLIPLPGGHEISGA
                     FDLLDSDWVLHDFKTSSKPYDPRRADRTQLVIYAWACERMFGRPPKALCFDVFVKGDG
                     ADGAVELQEPVLFPTPSPLEMAEVGRRLSAQIDQLIAAETRGAFPRSFVPLRCHWCEY
                     QTRCLTDWERDGRPEPVRVRLERLA"
     gene            complement(574..1182)
                     /locus_tag="PK362_01635"
     CDS             complement(574..1182)
                     /locus_tag="PK362_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73575.1"
                     /translation="MAATDRGDVLHRMDDFFAAYGRVRTVLRLAAESTRTGGDHRTRR
                     YRCVFESGEKPGPCLAGAREETRRTGSLVKIREEILFQLSRNLGDTGVLRVEADPVGR
                     WRGTMESVDPLLAEKDPEWPRANLAEMPGFPYRSLAGTGRADRLERYRSDRTRVADRL
                     AESETAGWTFSDRDGPIMHYLLFRVGARLELLGAVVGYDSYF"
     gene            complement(1182..3185)
                     /locus_tag="PK362_01640"
     CDS             complement(1182..3185)
                     /locus_tag="PK362_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC73576.1"
                     /translation="MTPLSPDWAPFLRKISLFAQLGEAALTRLRDRVHPRSAPAGTVL
                     FHQGDDSDSLIVLLAGEIRLSKIENGQDHLVTLLGRRGDTIGEMTLLNGRTRPVTARV
                     ERDAEFLVLERRDFESLLEENPALALPLARQLSEQLVQTAPVGVDRKSAVNRTYAFFA
                     PVPGPDRVVLGVNAALSLLEQSRRRVVVIDMAERPLDRVAPALGLRGAAAAADWRAED
                     FSTPDGLRRHIERHASGLDVLTLPLSLFDARFTESFYPLLHALRRDWDHIVFLLEGDM
                     GRLARALVAEVDRVLVAWDDPPSAAAGVLWRELEGVIAAPRLDRVELNGGDRIRRNRP
                     GHFMIPWPRGTGQRVLGGGTPFLGPADARTHRGLDRMARHLTGHRIGLAMGSGAALGY
                     SIIGILRVLERNGIYPDLISGTSMGALIGSFYAMGCTPDELEKIALSITKVKLWSMVD
                     FSLPPWKGIVIGNGVLKFLKSVLGDATFDDLELPFACVATDINTGDERILRHGAVAEA
                     VRASLSLPFFFQPFLHQGRYLVDGGLVNPVPSSVVRSMGADIRIGINITTKPAVKHFP
                     GFKSRLSPLDRLIVPNVVRVMMKTLYTMQYGIAQTRAHDSNVVLAPDLSAFTWMEFHR
                     AADIIRVGEDNAEHLLPKIKALLPFHNDQCRIPLRAPVAVRPY"
     gene            3319..4662
                     /gene="hisS"
                     /locus_tag="PK362_01645"
     CDS             3319..4662
                     /gene="hisS"
                     /locus_tag="PK362_01645"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNC73577.1"
                     /translation="MTRPTAPKGTRDIVGEELTRFRLVEDAARRLFGLHGFQEIRPPV
                     FESRDLFSRSVGETSDIVEKEMFSFEDRGGRSLALRPEGTAGVVRAFLEHGFDQNPRF
                     QKLFYLGPMFRAERPQAGRYREFWQAGAESFGNLSPWADAEMMFLAHGILKAAGVDVS
                     KLRFHVNTLGDAQCRSGYRDALVVYLNKWRGSLCEHCLRRIDKNPLRVLDCKVDGPKL
                     RDAGDVLPVMAEFLCSDCRAHDDDLLKILSDHQKRNKDFVFLKDPAIVRGLDYYVRTV
                     FEIKSTDLGAQDAVGAGGRYDGLVKQLGGPDLPGVGFALGLDRSAKLIRAEKLAEIQT
                     KRVFFAFDKDGATVEPWAAALLEAARMAVDGGPNGRRWEFDFGSRHQSLNGQLRRANS
                     WGADWVVFCLWDNGRPSVSVKFMRDAGGRQDAAPAAPDEFQKAFLKIIEERSPVQ"
     gene            4659..>5215
                     /locus_tag="PK362_01650"
     CDS             4659..>5215
                     /locus_tag="PK362_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNC73578.1"
                     /translation="MKRTTYCGLVTKKWEGQKVTLNGWVHNRRDHGGVIFVDLRDREG
                     LVQLVFHPEQKAIFAAGEALRGEAVVEVVGTVRPRPAGTVNPNLPTGEIEVWVEAVEV
                     LNVSRTPPFEISEHSNASEDVRLQYRYLDLRRPPLQKNLILRHKVTQTVRDVLAAQGF
                     LELETPFLTKSTPEGARDFLVPSRL"
BASE COUNT          956 a         1646 c         1720 g          893 t
ORIGIN      
        1 ctggaggcca tgggtcagca tttcctttcc cgctttctca gcgaacgggc ccccgccctg
       61 caaccggcgc cccggggcgt ggagtgccgg ttcctcatcc ccctgcccgg cgggcacgaa
      121 atctccggcg cctttgacct gttggacagc gattgggttc tccacgattt taaaacctcc
      181 agcaaaccct acgatccccg ccgggcggac cgcacccaat tggtcatcta cgcctgggcc
      241 tgcgaacgca tgttcgggcg cccgcccaaa gccctgtgct tcgacgtgtt cgtgaaaggg
      301 gacggcgccg acggcgccgt ggagttgcag gaaccggtgt tgttcccgac gccttcgccc
      361 ctggaaatgg ccgaggtggg ccgccggttg tcggcccaga tcgaccaatt gatcgccgcc
      421 gagacgcggg gggcttttcc ccgatccttc gtgccgcttc ggtgccattg gtgcgaatac
      481 cagacccggt gtttgacgga ttgggagcgg gacgggcggc cggaacccgt gcgggtccgg
      541 ttggaacggt tggcctagcc gggggaatcc ccgctagaaa tacgagtcgt atcccaccac
      601 ggcgcccagc agctccaacc gggcacccac gcgaaacaac aggtaatgca tgatcggacc
      661 gtcccggtcc gagaaggtcc agcccgcggt ctccgattcc gcgaggcggt cggcgacccg
      721 cgtccggtcc gatcgataac gttccaggcg gtcggcccgt cccgttccgg cgagggaccg
      781 ataggggaac cccggcatct cggcgagatt cgcccgcggc cactcggggt ccttctcggc
      841 gaggagcgga tcaacgcttt ccatcgttcc ccgccaccgt cccacggggt ccgcctccac
      901 ccgaaggacg ccggtgtccc ccaggttccg gcttagttga aataaaattt cctcccgaat
      961 cttcaccaac gaacccgtcc ggcgcgtttc ctcccgcgcc cccgccaggc acggccccgg
     1021 cttttccccg gactcgaaga cgcaccgata gcgtcgggtt cgatggtcgc cccccgtccg
     1081 ggtcgattcg gcggcgagac gaagcaccgt ccggaccctt ccgtaggcgg cgaaaaagtc
     1141 gtccatccgg tgaagaacgt cgccgcggtc cgttgcggcc atcagtaggg tcgaacggcg
     1201 accggcgccc gaaggggaat gcggcactgg tcgttgtgga acggcaggag ggctttgatt
     1261 ttcggcagca ggtgttccgc gttgtcttcg cccacgcgga tgatgtccgc ggcccggtga
     1321 aattccatcc aagtgaaagc cgacaagtcc ggcgccagca ccacgttgga atcgtgggcc
     1381 cgggtctggg cgatgccgta ttgcatggtg tagagggttt tcatcatcac ccggacgacg
     1441 ttgggaacga tcagccggtc cagaggggac aagcgggatt tgaaaccggg aaaatgtttg
     1501 acggcgggtt tcgtggtgat gttgatgccg atccggatgt ccgcgcccat ggagcgcacg
     1561 acggaggacg gcacgggatt tacgagaccg ccgtccacca ggtagcggcc ctggtggaga
     1621 aagggctgga agaaaaaagg gagggagagg gaggcccgca ccgcttcggc cacggccccg
     1681 tggcggagga tccgttcgtc cccggtgttg atgtccgtgg cgacgcaggc gaagggaagt
     1741 tccaaatcgt cgaaggtcgc gtcgcccagg accgatttga ggaacttgag caccccgttg
     1801 ccgatgacga tccccttcca gggggggagc gaaaaatcca ccatcgacca caatttgacc
     1861 ttggtgatgc tgagggcgat cttttccaat tcgtcgggcg tgcagcccat ggcgtagaaa
     1921 ctgccgatca gggcgcccat ggaagttccc gagatcaggt cggggtagat tccgtttcgt
     1981 tccagcacgc gcaaaatccc gatgatggaa tacccgagcg ccgccccgga gcccatggcc
     2041 aagccgatgc gatggcccgt caagtggcgg gccatccggt cgaggccgcg gtgcgtccgg
     2101 gcgtccgcgg gccccaaaaa gggcgtgcca ccccccagga cccgttggcc cgttccccgg
     2161 ggccagggaa tcatgaaatg gccgggccgg ttccgccgga tccggtcgcc gccgttcaat
     2221 tccacgcgat ccaaacgcgg ggcggcgatc acgccttcca gttcccgcca caaaacgccc
     2281 gccgcggcgg aggggggatc gtcccaggcc accaggaccc ggtcgacttc ggccaccagg
     2341 gcccgggcga ggcgacccat gtcgccctcc aggagaaaaa cgatgtggtc ccaatcccgt
     2401 cgaagggcgt ggagcagggg gtaaaagctt tcggtgaacc gcgcgtcgaa aagggagagg
     2461 gggagggtca acacgtcgag ccccgaggcg tgccgttcga tgtgacgccg aagtccgtcg
     2521 ggggtggaaa aatcttcggc acgccaatcg gccgccgcgg cggcgccccg aaggcccagg
     2581 gccggggcca cccgatcgag gggccgctcc gccatgtcga tgacgaccac ccgccggcgg
     2641 ctttgttcca acagggaaag ggccgcgttg acgcccagga ccacccggtc gggccccggc
     2701 acgggagcga aaaaggcgta cgtccgattg accgccgatt ttcgatccac cccgacgggc
     2761 gcggtttgaa ccaattgttc ggacaattgg cgggccaggg gcagggccaa ggcggggttt
     2821 tcctccaaga gagattcgaa atcccggcgt tccagcacca ggaattccgc gtcccgctcc
     2881 acccgggcgg tgaccggccg ggtgcggccg ttcaggagcg tcatttcgcc gatcgtgtcc
     2941 ccccgtcggc ccaggagggt gaccaggtgg tcctgcccgt tttcaatttt ggaaagccgg
     3001 atttcgccgg ccagaaggac gatcagggaa tcgctgtcgt ccccttggtg gaagaggacg
     3061 gtgccggccg gggccgagcg gggatggacc cggtcccgaa ggcgcgtcag ggccgcttcc
     3121 cccaactggg cgaagaggga aatcttgcgg agaaaaggcg cccaatcggg cgacaaaggt
     3181 gtcatcgcgt ggtctccgga gcgccccggg ggacgctccc accctaaggg aaggcccgaa
     3241 acgggggttt gtcaaggcgg cggggcccga aatccttccg ggggaaaatg ataaaatcgg
     3301 gtctccaaaa atccgcccat gacccgaccc accgccccca aaggtacccg cgacatcgtc
     3361 ggcgaggaat tgacccgttt ccggttggtc gaggacgcgg cccgacgcct cttcggcctt
     3421 cacgggttcc aggagatccg cccccccgtg tttgaatccc gggacctgtt ttcccgatcc
     3481 gtgggcgaaa cctccgacat cgtggaaaaa gaaatgtttt ccttcgagga ccgggggggc
     3541 cgttccctgg cgctccggcc cgaaggcacg gccggcgtgg tgcgggcttt cctcgagcac
     3601 gggttcgacc aaaacccccg gtttcaaaaa cttttttact tggggcccat gttccgcgcc
     3661 gagcggcccc aggccggccg ttaccgggaa ttttggcagg cgggggccga atcctttgga
     3721 aatttgtcgc cctgggccga cgccgaaatg atgtttttgg cccacggaat cctcaaggcc
     3781 gccggcgtgg acgtgtcaaa gctccgcttc cacgtgaaca ccctgggcga cgcccagtgc
     3841 cggtcgggtt atcgcgacgc cctcgtcgtt tatttgaata aatggcgggg ttccctctgt
     3901 gagcactgtc tccggcgaat cgataaaaac cccctgcggg tactcgattg caaagtcgac
     3961 gggcccaagc tgagagacgc gggggatgtt ttacccgtga tggcggaatt cctttgttcg
     4021 gattgccggg cccacgacga tgatttgctg aaaatacttt cggaccacca aaagcgtaat
     4081 aaagactttg tttttttaaa agaccccgcc atcgtgcggg ggttggatta ttacgtgcgg
     4141 acggtgtttg aaatcaaatc gaccgacctg ggggcccagg acgcggtggg ggccgggggc
     4201 cgctacgacg gactcgtgaa acagctgggc ggccccgacc tgcccggcgt ggggttcgcc
     4261 ctggggttgg atcggtccgc caagctgatc cgggccgaaa aattggccga gatccaaacc
     4321 aagcgggtct tcttcgcctt cgacaaggac ggcgcgaccg tcgaaccctg ggccgccgcg
     4381 cttctggagg ccgcccggat ggccgtggac ggcgggccga acggccgccg atgggaattc
     4441 gatttcggct cgcggcatca aagcctgaac ggtcaattgc ggcgggccaa ttcctggggc
     4501 gccgactggg tcgtgttttg cctgtgggac aacggccgcc ccagcgtgtc cgtcaaattc
     4561 atgcgcgacg cgggcggacg ccaggacgcc gcgcccgccg cgccggacga atttcaaaaa
     4621 gcttttctaa aaatcatcga ggagcgctcc ccggtccaat gaaacgtaca acctattgcg
     4681 gtctcgtcac caagaaatgg gaaggccaaa aagtcacgtt gaacgggtgg gtgcacaacc
     4741 gccgggacca cggcggcgtc attttcgtgg atttgcggga ccgggagggg ttggttcaat
     4801 tggtgttcca ccccgagcaa aaggcgatct tcgcggcggg ggaggccctg cggggcgagg
     4861 cggtcgtgga agtcgtcggc accgtccggc cccgtccggc ggggaccgtg aatccgaatc
     4921 tgcccaccgg ggaaatcgaa gtctgggtgg aggccgtgga agtgctgaac gtcagccgca
     4981 cgccgccctt cgaaatctcc gagcacagca acgccagcga agacgttcgc ctgcagtacc
     5041 gctacctcga cctgcgtcgg ccgccccttc aaaagaattt gatccttcgc cacaaagtga
     5101 cccagacggt gcgggacgtc ctggccgccc aggggttcct ggaattggaa acgccttttt
     5161 tgaccaagtc gacgcccgag ggcgcccggg acttcctggt gccctcccgg ttaaa
//
LOCUS       DAOQLS010000053         5197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7278_length_5197_cov_3.793466, whole genome shotgun sequence.
ACCESSION   DAOQLS010000053 DAOQLS010000000
VERSION     DAOQLS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7278_length_5197_cov_3.793466"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="hemW"
                     /locus_tag="PK362_01655"
     CDS             complement(<1..905)
                     /gene="hemW"
                     /locus_tag="PK362_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNC73579.1"
                     /translation="MAPLPPKNHPLTGDADPGRSAPSARAETGLYLHIPFCDVKCRFC
                     DFAAYPGRKNDIPRYRAALERDLRDRAREFPDRRLATFYVGGGTPTVYSAAELTALAN
                     LVTTLFPAAPDWEATLEANPEGAAPEKMAAARAAGFNRLSLGLQSSDDRLLKTLGRLH
                     TFDGFKTAFRTARAAGFDNVNVDLMFGLPGQTADGWRDTLDRVLALAPDHLSAYALTV
                     EDATYFKKSGVASDPDFQAELYEIAADRLAAAGFVHYEISNFARPGKESRHNLRYWRN
                     VECLGAGVSAAWYVGGRRRKNHESLT"
     gene            complement(872..1816)
                     /gene="lepB"
                     /locus_tag="PK362_01660"
     CDS             complement(872..1816)
                     /gene="lepB"
                     /locus_tag="PK362_01660"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNC73580.1"
                     /translation="MEEKLFWISMVSGVYGLALKAFMKVETRVSDWRRVALMNGVFWM
                     LTALVVTTLLVSYKEWPAPSALSLILLAVGGFAAGVASRAVRSRSREISVAVLRDNLE
                     WADTGFSAILLAAVIMYFLVQAFKIPSGSMRNTLLEGDHLFVNKLVYGVRIPYTGRRV
                     LRFRAVKRGDVVVFRFPTTDKASPHYGKDFIKRAIGLPGDTIEVRDKKVFVNGASTEE
                     TYTHFADEEVLPGPEPSLSGADRQAVWERGDLAREEGENVRDNFGPVTVPPGHYFCMG
                     DNRDRSYDSRFWGPMPDTHLKGRAWFVYWPLSRRKIIR"
     gene            complement(1819..3630)
                     /gene="lepA"
                     /locus_tag="PK362_01665"
     CDS             complement(1819..3630)
                     /gene="lepA"
                     /locus_tag="PK362_01665"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNC73581.1"
                     /translation="MNPENIRNFSIIAHIDHGKSTLADRLLEETGTIEKREMRAQILD
                     GMELERERGITIKAKAVRMHWRHQQRDYVLNLIDTPGHVDFTYEVSRALAACEGALLL
                     VDASQGVEAQTLANAHLAQEAGLTIIPVINKIDLPSSDVEGVKKQLQESLGIGGPYFL
                     TSAKEGKGVPEILQAIVERVPPPTGDPARPLSALAFDSVYDPYRGVIVYVRLLDGQIK
                     PGLKVRFVATGAVHVVEEVGYLKPKYVKAELLSTGEAGYVVCGIKDIHQVKAGDTLTE
                     DARPTESPHPGYREMKPFVFAGFYPLAQADYENLKKAIEKLHLEDAALFHQPENSVAL
                     GFGFRCGFLGLLHMDIIQERIRREFGVDLLVTAPTVVYRVTDLRGKVAEFDSPAKFPP
                     HGDIVQIDEPYVLSTVVVPSDFVGPVMQLCQDRRGQFLDMRYLTPTRVVLKYEIPLAE
                     IVVDFYDALKSLSKGYASFDYEQAGYRPGDLQRMDILINNEEVDAFSFVVPTVQAAYR
                     GREIAERLRELIPRQMFEVPIQARVGSRIVARETVRAMRKDVLAKCYGGDISRKRKLL
                     EKQKEGKKRMRQFGQVEIPQEAFLAVLRRDDSGKKEE"
     gene            complement(3644..>5197)
                     /gene="ptsP"
                     /locus_tag="PK362_01670"
     CDS             complement(3644..>5197)
                     /gene="ptsP"
                     /locus_tag="PK362_01670"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNC73582.1"
                     /translation="QQKILKLLGKTHARLIEAYILILEDPLIAKDVVKNISQNRVNAE
                     YALSEAIAGAVKALESATDEYFRERRHDVLDVGRKILLHLLGHEPQSLDDLKEPSVIV
                     ARNLTPSDTIHMQTKFAEGFATDIGGRTSHTAILANSMGIPAVVGLHDISRHVRPGDM
                     LIVDGHTGTVVINPSPDLVDNYRRERDIRLAEKRDLEGLRDQPAQSTDGRRLTLAANI
                     DSPTEMKGVLSNGAEGVGLLRTEFLYLNRAALPTEEDHLQFYTRVAQSVLPYPVIIRT
                     VDIGGDKLVDLGFAHLSKSEPNPFLGLRGIRLSLKHHELFRTQLRAILRASAVGKVKV
                     MFPMVSGVEEFRAGRKILAECMEELRRENKPFDEKMEVGLMIEVPSAALVVDHLAQEA
                     DFMSVGTNDLIQYTLAVDRVNDEVAHLYEPLHPAILRLLSQTVKAAHAAGKWVGMCGE
                     MASDLDVTPILLGLEFDELSVSAGLVPKVKKMIRDLSFTDCQALAQEVLKDPTRDNVQ
                     RLLRQFQKR"
BASE COUNT          952 a         1628 c         1652 g          965 t
ORIGIN      
        1 gtcaacgatt cgtgattttt ccgacggcgc cccccgacgt accaggccgc cgaaaccccc
       61 gcccccaggc actccacgtt gcgccaataa cgaaggttgt ggcggctctc ctttcccggc
      121 cgggcgaaat tgctgatttc gtagtgcaca aaccccgccg ccgccaaccg atcggcggcg
      181 atttcgtaaa gctccgcctg aaaatccgga tccgacgcca cgccggactt cttgaaatag
      241 gtggcgtcct ccaccgtgag cgcgtaggcc gaaaggtggt cgggggccag ggccagcacg
      301 cggtccaggg tgtcccgcca gccgtcggcc gtctggccgg gcagaccgaa catcaaatcc
      361 acgttgacgt tgtcgaaccc cgccgcgcgg gccgttcgaa aagccgtttt aaatccgtcg
      421 aacgtgtgga gccgcccgag ggttttcaac aagcggtcgt cggacgactg caggcccagg
      481 ctgagccggt tgaacccggc ggcccgcgcg gcggccattt tttcgggcgc ggccccttcg
      541 ggattggcct cgagggtggc ctcccaatcc ggcgcggccg gaaacagcgt cgtcacgagg
      601 ttcgccagag ccgtcaattc ggccgccgaa tagaccgtgg gggtcccccc cccgacgtaa
      661 aaagtcgcca accgccggtc cggaaattcc cgggcgcggt cccgcaaatc ccgctccagc
      721 gccgcccggt accgggggat gtcgtttttc cggccgggat aagccgcgaa gtcgcagaag
      781 cggcatttca cgtcgcagaa ggggatgtgc agatagaggc ccgtctccgc gcgggcggag
      841 ggggcggagc ggccggggtc ggcgtccccg gtcagcggat gatttttcgg cgggagaggg
      901 gccaatacac gaaccaggcc cggcccttca ggtgggtgtc gggcatggga ccccaaaaac
      961 gggaatcgta ggaccggtcg cggttgtcgc ccatgcagaa gtaatggccc ggcggtacgg
     1021 tgacggggcc gaaattgtcc cgcacgtttt cgccttcctc ccgcgccaaa tcgcctcgtt
     1081 cccagacggc ctggcggtcg gccccggaaa gcgacggttc ggggcccggc aggacttcct
     1141 cgtccgcgaa atgggtgtag gtttcctcgg tcgacgcccc gttgacgaag actttcttat
     1201 cgcgcacttc gatggtgtcg cccggaagcc cgatggcgcg tttgatgaaa tccttgccgt
     1261 aatgggggct cgccttgtcg gtcgtgggaa aacgaaacac caccacgtcc ccgcgcttga
     1321 ccgcgcgaaa gcggagcacg cgccggcccg tgtaggggat tcgaacgccg tagaccaatt
     1381 tgttaacgaa aagatggtcc ccctccaaca gggtgttgcg catggacccg gaggggattt
     1441 taaaggcctg gacgaggaaa tacatgatga cggccgccag caggatggcc gaaaaacccg
     1501 tgtcggccca ttccaggttg tcgcgcagga ccgcgacgga gatctcccgg gaccgggatc
     1561 gaacggcccg cgacgccacg cccgcggcga acccccccac ggcgaggagg atcaaagaca
     1621 gggccgacgg cgccggccat tccttgtagg acacgagcag cgtcgtcacc accagcgccg
     1681 tcagcatcca aaacaccccg ttcatgagcg cgacccggcg ccaatccgac acccgggtct
     1741 caactttcat gaacgccttg agggccaggc cgtagacccc gctcaccatg gaaatccaaa
     1801 acagtttttc ttccatcgtc attcctcttt cttgccggaa tcgtcgcgtc ggagaacggc
     1861 caaaaacgcc tcttggggaa tctccacctg cccgaactgc cgcatgcgtt ttttcccctc
     1921 tttttgtttc tccaacagtt tgcgcttgcg ggaaatgtcg ccgccgtagc atttcgccag
     1981 cacgtccttg cgcatggcgc ggaccgtttc ccgggcgacg atgcggctgc cgacccgggc
     2041 ctggatgggc acttcgaaca tctggcgcgg aatcagctcc cgcaggcgtt cggcgatttc
     2101 ccggccccgg taggcggcct ggacggtggg aacgacgaag gaaaaggcgt ccacttcctc
     2161 gttgttgatc aaaatatcca tgcgttggag gtcgccgggg cgataacccg cctgctcgta
     2221 gtcgaaggag gcgtagccct tggacaagga cttgagcgcg tcgtaaaaat ccacgacgat
     2281 ttcggccagg gggatctcgt atttaagaac cacccgggtc ggcgtcagat aacgcatgtc
     2341 caggaactgg ccccgccgat cctggcacaa ctgcatgacc ggccccacga aatccgacgg
     2401 gaccacgacg gtggacagca cgtacggctc gtcgatctgg acgatgtcgc cgtggggcgg
     2461 aaacttggcg gggctgtcga attccgccac ctttccccgc aaatccgtca cccggtagac
     2521 caccgtcggg gccgtgacca ggagatccac gccgaactcc cgccggatcc gctcctggat
     2581 gatgtccatg tggagaaggc ccaaaaaccc gcaccgaaac ccgaacccga gggccacgga
     2641 gttctccggc tgatggaaaa gcgcggcgtc ctcgaggtga agtttttcga tggctttttt
     2701 taaattctcg tagtccgctt gggccagggg atagaacccc gcgaagacga agggtttcat
     2761 ttcccggtag ccggggtggg gcgattcggt ggggcgggcg tcctccgtca aggtgtcgcc
     2821 ggccttcacc tggtgaatgt ccttgatgcc gcaaacgacg taccccgcct ccccggtgga
     2881 aaggagttcc gccttaacgt atttgggttt gagatacccc acttcctcga cgacgtggac
     2941 ggcgccggtg gcgacgaagc ggactttgag gcccggtttg atctgcccgt ccagcaagcg
     3001 gacgtaaacg atcacgccgc ggtacgggtc gtagaccgaa tcgaacgcca gggccgagag
     3061 cggccgcgcc ggatcgcccg tcgggggcgg cacgcgctcc acgatggcct gaagaatttc
     3121 cggaacgcct ttgccttcct tggcgctggt gaggaaatag ggcccgccga tccccaggga
     3181 ttcctgaagc tgctttttaa ccccctccac atcggaggac ggcaaatcga tcttgttgat
     3241 gacgggaata atcgtgagcc cggcttcctg ggccaggtgg gcgttggcca gggtttgggc
     3301 ctccacgccc tggctggcgt ccaccagcag caaagcgcct tcgcaggccg ccaaggcccg
     3361 gctgacctcg taggtgaaat ccacgtggcc cggggtgtcg atcaaattaa gaacgtagtc
     3421 ccgttgctgg tgccgccaat gcatgcggac ggccttggcc ttgatggtga tgccccgctc
     3481 ccgctccaac tccatgccgt ccaggatctg ggcccgcatt tcgcgtttct cgatggtgcc
     3541 ggtctcctcc aacagacggt cggccagggt gcttttcccg tggtcgatgt gggcgatgat
     3601 cgaaaagttg cggatgtttt ccggattcat ggcgttcggc gaatcagcgt ttttggaact
     3661 ggcgaaggag ccgttgaacg ttgtcgcggg tgggatcctt gagcacttcc tgggccaggg
     3721 cctggcagtc ggtgaaagag aggtcccgga tcattttctt gactttgggg accaagcccg
     3781 ccgagaccga gagctcgtcg aactcgaggc ccaggaggat gggcgtgacg tccaaatccg
     3841 acgccatctc gccgcacatt cccacccact tgccggccgc gtgggcggcc ttgacggtct
     3901 gggacaacag ccggaggatg gcggggtgca ggggctcgta caaatgggcc acctcgtcgt
     3961 tgacgcggtc cacggcgagg gtgtattgga tcaaatcgtt cgtgcccacg gacatgaaat
     4021 cggcctcctg ggcgaggtgg tccaccacca gcgccgccga cggaacctcg atcatgaggc
     4081 ccacttccat cttctcatcg aagggcttgt tttcccgccg gagttcctcc atgcattcgg
     4141 ccagaatttt ccggccggcg cggaattcct ccacccccga caccatcggg aacatgactt
     4201 tcaccttgcc gacggccgac gcccggagaa tcgcccgcaa ctgcgtccgg aacaattcgt
     4261 ggtgcttgag cgacaggcga atgccccgaa ggcccaggaa cggattcggc tcggacttgc
     4321 tcaaatgagc gaaccccaaa tccacgagct tatcgccccc gatgtcgacg gtccgaatga
     4381 tgacggggta gggaaggacc gattgggcca cccgggtgta aaattgaaga tgatcttctt
     4441 ccgtgggaag cgcggcccgg ttcaaataca ggaattcggt gcgaagcaac cccaccccct
     4501 ccgccccgtt cgaaagcacg cccttcattt ccgtgggcga atcgatgttc gcggccaggg
     4561 tgagccggcg gccgtcggtg gattgggccg gctggtcccg caggccctcc aaatcgcgct
     4621 tttcggcgag gcggatgtcc cgctcgcggc ggtagttgtc gacgaggtcc ggcgaagggt
     4681 tgatgacgac cgtgccggtg tgcccgtcca cgatgagcat gtcgcccggg cggacgtggc
     4741 ggctgatgtc gtgcaggccg accacggccg ggatccccat ggaattggcc aggatggcgg
     4801 tgtgggaggt tcggcccccg atgtcggtgg cgaagccctc ggcgaacttg gtctgcatgt
     4861 ggatcgtgtc cgacggagtc aggttccgcg cgacgatcac ggacggttct ttcaaatcgt
     4921 ccagggattg gggctcgtgg ccgagcagat ggagcaaaat ttttcgcccg acgtccaaca
     4981 cgtcgtgccg ccgctcgcga aaatattcgt cggtggccga ttccagcgcc ttcacggcgc
     5041 cggcgatggc ctcggagagg gcgtattctg cgttgacccg gttttgactg atgttcttga
     5101 ccacgtcctt ggcgatcagc gggtcttcca aaatcaggat gtacgcctcg atcagccgcg
     5161 cgtgggtttt cccgagaagc ttcagaatct tctgctg
//
LOCUS       DAOQLS010000054         5182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7321_length_5182_cov_3.701970, whole genome shotgun sequence.
ACCESSION   DAOQLS010000054 DAOQLS010000000
VERSION     DAOQLS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7321_length_5182_cov_3.701970"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PK362_01675"
     CDS             complement(<1..551)
                     /locus_tag="PK362_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73583.1"
                     /translation="MTKRKSNARGSLVVLLVVGMALLMLLIPPLTQWLRKDAKDAVRY
                     QRALQSSNLAEAALEKVEWRLSGDAGLAAALVAGSAPAGFQGDVEYNDIEGGVYKVNV
                     ALSTSVHSTGFTLTIRAKENRTQVVKAVQATYVKPEFKVAAPVGYISANGIFADIHWS
                     PIVASYISGPGMSLPDYPRLLSGG"
     gene            complement(604..3435)
                     /locus_tag="PK362_01680"
     CDS             complement(604..3435)
                     /locus_tag="PK362_01680"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNC73584.1"
                     /translation="MSPSRSGRRRFRFLFWPWGTGFSLVELLVGFALLSAVFLVGSRF
                     FSKISVGVTRSRSGTTAAQLTERTLNRMLASPINRLLVGTTLTAIPGIDVALNADVVN
                     YPPETSQVGSTSFVTYALVEKVIQNGAGQSLTVVASPGDAETNMRRITVTTVWTENGH
                     TRRYTSAKVKQVGLDLETPAVLRGTVTDNLGTPLRGVQINVLQSHIGSSGGNYRNYDG
                     KYTRSDGTYELQVYSGIYSVEARLPGKDSQIATDVRITTAISPVQLNFTLQPRSTGQM
                     SGKIWYHLGPVISQVVGDTVTASGFHQEYVELFLAEAQVNNLWSNNYGYTLSLPSAIW
                     RRWGLRFQRRTGQDASAKPIKLRWIDEPPVSGTYYYERPYVIFANTNPVVINGVSRQV
                     HAVWDTTTTGVGSNFDYWGVAAGGHFTAADPNIFPVAEDGVGGTTPEAEGAGALELYD
                     TTLGLVYDRVGWSRGPGLDPAFSEGTAIQGGGTGLRRGEQFVRYSSTAGIDRNYGSAY
                     DSGNNDLDFGDVFPMTYRPFEWQDNTNLSGAYWQAGAAPRCLAGTVDPTLILTGGDEM
                     SDVVRGTVTTVGGRPVVNYSLTMGTGTYPVFWSTALGRGYTTAFDNSYKQFYIARGTS
                     VVTDLHAYNYQFINPGFFPGGSVTEGTYRNGLGMPVPNVAVAGQTRNAGGGVPTIALD
                     NNAFYPMNAVKVTNAAGFYRTLSWGGLYISNNDSEAQYVNFASTPGGNTPLDLVRNAT
                     AFLRGRVVLSDGVSPAEGFLVKIYYCCSRSTVRNQTSVQYNGYFDTYVGTGTWEIVPT
                     NNTNYTINEITSPESATVSVGFVDEGNTIFAGSFTVVNNACQVAGRVTNGGAPVASGV
                     LVTAVPAAVNASFLTALSTTGATTNAGWVSALSDERGNYQIRAPTPGNYNLYAFFSDL
                     VDGTVTVRQSTPTAVVLSSGSTLTSDINF"
     gene            complement(3640..4170)
                     /locus_tag="PK362_01685"
     CDS             complement(3640..4170)
                     /locus_tag="PK362_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73585.1"
                     /translation="MQKNNSDRRVVLVGVYATPLAFILGTLGIFLATIPEKEQLICAG
                     VLGFTVFYNVVFPFFFKNLSQDLRGINVNIRLYLNLAVNGVLVYFLGDSFRPIWLILA
                     LTPIASAIYGSRRNTLMKAAVVSALLVGIQAARPDRTWLAWGEQAAHISFIVLISLLV
                     NRLASLISPQPATASI"
     gene            4356..>5182
                     /locus_tag="PK362_01690"
     CDS             4356..>5182
                     /locus_tag="PK362_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73586.1"
                     /translation="MPIPPPPASNAPCPEDGQGPLLSAEAGLSLIELSLSMAVMGFLV
                     MGTVLAFQWGLRASLGARQQMAGNNLLQSIHTRLLNMDFYNVFSVNSSSPNFGLWAAY
                     PEKTALTTLWQSAQNVGFTGFAIDVAFMRRDLSDANGNGLTTDLVPFTDVNHDGVDDF
                     DPNLRYFDQNADGDFYDRYGPTNLPEQPDTHIKKLDIVLYKGAAAVASRKGDLLSLEQ
                     FSGSESASSEAAFALRVDAPDNSAVLYRAVTPAQTAALNLPVAKSFPASPAAVRADAG
                     "
BASE COUNT         1054 a         1610 c         1539 g          979 t
ORIGIN      
        1 ccgccggaaa gaaggcgggg gtagtcgggc aacgacatcc ccggaccgga aatataactt
       61 gcgacgatgg gcgaccaatg aatgtcggcg aagatgccgt tggcgctgat gtagcccacc
      121 ggcgccgcga ctttaaattc gggcttcacg taggtcgcct gaaccgcttt aacgacctgg
      181 gttcgatttt ctttcgcccg aatcgtgagc gtgaatccgg tggagtggac ggaggtgctc
      241 agcgccacgt tcaccttgta gaccccgccc tcgatgtcgt tgtattccac gtccccctga
      301 aaacccgcgg gggcggatcc ggccacgagc gcggccgcga gccccgcgtc gccggaaagc
      361 cgccattcca ctttttccag ggcggcttcc gccaggttgg aggattggag ggcccgttga
      421 taccgaacgg cgtctttcgc gtccttccgc agccattggg tcaacggcgg aatgagaagc
      481 atgagcaacg ccatgcccac caccaagagg accaccaacg agccccgtgc gttgcttttc
      541 cgcttcgtca taccgcgatc ctcgctttta aacccattct cggaatccgt cggccggatc
      601 gaatcaaaaa ttgatatcgc tcgtcagcgt cgaacccgac gaaaggacca cggccgtcgg
      661 ggtgctttgg cgcacggtca ccgtcccatc caccaagtcg gagaaaaacg cgtagaggtt
      721 gtaatttccc ggggttggcg cacgaatttg atagttcccg cgttcgtccg agagtgcgct
      781 cacccacccc gcgttggtgg tggcccccgt cgtggagagc gccgttaaga aagacgcgtt
      841 caccgcggcc ggaaccgccg tcaccaacac gcccgacgcc accggggccc ccccgttcgt
      901 cacccttccc gccacctggc aggcgttgtt gacgaccgtg aacgaaccgg caaagatcgt
      961 gttgccctcg tccacgaaac ccacgctgac ggtggcgctc tcggggctcg tgatttcgtt
     1021 gatggtgtaa ttcgtgttgt tggtgggaac aatttcccac gttccggtgc cgacgtaggt
     1081 atcaaaatag ccgttgtatt ggacgctcgt ctggttccgc accgtcgaac gggaacagca
     1141 gtagtatatt ttcaccagga atccctcggc gggggagacc ccgtcggaca agaccacccg
     1201 ccctcgaagg aacgcggtcg cgttgcgaac cagatccagc ggcgtgttcc cgccgggggt
     1261 cgaggcaaaa ttcacgtatt gggcctcgct gtcgttgttg ctgatgtaaa ggccgcccca
     1321 gctgagggtc cgataaaatc cggccgcgtt ggtgaccttc acggcgttca tggggtaaaa
     1381 agcgttgttg tccagcgcga tggtcgggac gcccccgccg gcgttgcggg tttgtcccgc
     1441 gacggcaacg ttgggcacgg gcatccccaa tccgttccga tacgtcccct cggtgacgct
     1501 tcccccgggg aaaaacccgg gattgataaa ttgatagttg taggcgtgaa gatccgtcac
     1561 caccgaggtt ccccgagcga tataaaattg tttgtagctg ttatcaaacg cggttgtgta
     1621 gccgcggccc agcgcggtgg accaaaagac gggataagtg cccgtcccca tcgtcaaact
     1681 gtaattcacc accggacgcc cgcccaccgt ggtgacggtt ccgcggacga catcggacat
     1741 ttcatccccg cccgtcagaa tgagcgtcgg gtcgaccgtt ccggccaaac agcggggggc
     1801 cgctcccgcc tgccagtagg cgccggaaag gttcgtgttg tcctgccatt caaaaggccg
     1861 ataggtcatc gggaacacgt cgccaaaatc caggtcgttg ttgccgctgt cgtaggccga
     1921 tccgtagttg cggtcaatgc ccgcggtgct ggaataccga acgaactgtt cgccccgccg
     1981 cagtcccgtt ccaccgcctt gaatcgccgt cccttcggaa aaagccgggt ccaatcccgg
     2041 gccccgactc caccccacac gatcgtaaac caggcccagc gtcgtgtcgt acaactccaa
     2101 ggcaccggcg ccctccgctt ccggggtcgt gccgccgacc ccgtcctcgg ccaccgggaa
     2161 aatgttcgga tccgccgccg tgaaatgccc ccccgccgcc accccccagt aatcaaaatt
     2221 gctgccgacg ccggtggtcg tcgtgtccca cacggcgtga acctgccggg agacgccgtt
     2281 gatcaccacc ggattggtgt tggcgaaaat cacatagggg cgttcataat aataggtgcc
     2341 gctgacgggc ggttcatcaa tccaacgcaa tttgatgggc ttggccgagg cgtcctgccc
     2401 ggtccgacgc tgaaatcgaa gaccccagcg ccgccagatg gcgctgggaa gggacagggt
     2461 gtaaccgtag ttgttggacc aaaggttgtt gacctgggcc tccgccaaaa aaagttcgac
     2521 gtattcctgg tgaaagcccg acgcggtcac cgtgtccccc accacctggg agatgacggg
     2581 gcccaggtgg taccatattt tcccggacat ctggcccgtg ctccgcggtt gcaaagtgaa
     2641 attcaattgg acgggggaga tcgccgtggt gattcgaacg tccgtcgcga tctgggagtc
     2701 cttgcccggg agacgcgcct ccacgctgta tattccggaa tacacctgca actcgtaggt
     2761 gccatcggat cgggtgtatt taccgtcgta gttgcggtaa ttgcctcccg agctaccgat
     2821 gtgggattga agaacattga tttggacgcc ccgcaggggc gttcccaggt tgtccgtgac
     2881 ggtgccgcga agaacggccg gggtctcaag atccaacccg acctgtttaa ccttggccga
     2941 ggtgtaccga cgcgtgtgac cgttttcggt ccaaaccgtg gtcaccgtga tgcgccgcat
     3001 gttcgtctcc gcatcgccgg gggacgccac gaccgtgagc gactggccgg cgccgttttg
     3061 aatgactttt tcgaccaagg cgtatgtcac gaacgaggtg gaaccgacct gggacgtttc
     3121 cggcgggtaa ttcaccacat cggcattcaa cgccacatcg atgcccggga tcgcggtgag
     3181 ggtcgtcccc acgagcaacc gattgatcgg ggaggccaac attcggttga gcgttcgttc
     3241 ggtcaattgc gccgccgtgg ttcccgagcg ggaacgggtc acgccgacgc tgattttgga
     3301 gaaaaaccgg gatccgacga ggaacaccgc cgacaggagg gcgaagccaa caagcaattc
     3361 caccagggaa aatccggttc cccagggcca aaaaagaaaa cggaatcgtc gacgaccgct
     3421 gcgagagggg ctcatggacg ccttttccag gcctttaagg ctatccccag tggggggata
     3481 atgcaaggga gcggccgggg ccgtccggga aacggcaacc gcccggaggc tcctttgccc
     3541 gttcgatgat gggaaaggaa accggccgga gtgggttctt aagacgcgct taagttaatc
     3601 gggccggaag ggggggcggg aaaggacgtt taaaattatt taaatcgacg ccgtcgccgg
     3661 ctggggtgaa atcaaggagg ccaaccggtt gacgagcagg ctgatcagaa cgatgaagga
     3721 aatatgggcg gcctgttccc cccaggcgag ccaggtgcgg tccggtcggg cggcttgaat
     3781 ccccaccaac agggccgaca ccacggcggc cttcatcaac gtgttccggc gcgatccgta
     3841 aatggccgag gcgatgggcg tcagcgccaa gatgagccaa atgggccgga aactgtcccc
     3901 caggaaatac accagaacgc cgttgacggc caagttgaga tacaaacgga tgttgacgtt
     3961 gatcccccga aggtcctggg aaaggttttt aaaaaagaag ggaaacacca cgttgtagaa
     4021 aacggtaaac ccgagaaccc ccgcgcaaat caattgttct ttctcgggga tcgtcgccag
     4081 aaaaatcccc agggtcccca ggatgaacgc caagggcgtg gcgtagacgc cgacgaggac
     4141 gacccgccgg tcggagttgt ttttttgcac ggcttgattc atggatggcc taccttaaaa
     4201 aaagattgcg agcgccgccc gcgagcgcca cgggggaatt gtaacctacc cgcggacccc
     4261 tggctagtcg cggcgggccg tcgaaccggc cttccgctca cgcgccgccg gtgatctggt
     4321 atattatgtc gacgttaaaa accaggagaa tgaccgtgcc gattcccccg ccccccgcgt
     4381 ccaacgcgcc gtgccccgaa gacggccagg gacccctcct gtccgccgaa gcgggcctct
     4441 ccctcatcga attgtccttg tccatggccg tcatggggtt tttggtcatg ggaacggtgc
     4501 tggccttcca atggggattg cgggcctcct tgggcgcccg gcaacaaatg gcgggaaaca
     4561 atcttctgca gtccatccac acccgccttc tcaacatgga tttctacaac gtcttttcgg
     4621 taaattccag cagtccgaat ttcggcctgt gggccgctta cccggaaaaa accgcgctca
     4681 ccaccctgtg gcagtcggct caaaatgtgg gattcacggg attcgccatc gacgtagcgt
     4741 ttatgcgccg ggacctctcc gacgccaacg gcaacggatt gacgacggac ctggtcccgt
     4801 tcacggacgt caatcacgac ggggtggacg acttcgaccc caacctccgc tattttgatc
     4861 aaaacgccga cggagatttc tacgaccgtt acggccccac caatctcccg gaacaacccg
     4921 acacccacat taaaaagctc gacattgttc tctacaaagg ggccgcggcc gtggcttcac
     4981 gcaaaggcga tttgttgtcc ctggagcaat tcagcggatc cgaaagcgcc tcctccgaag
     5041 ccgccttcgc cctgcgcgtc gacgcccccg acaactccgc cgttctttac cgggcggtca
     5101 ctcccgccca aaccgccgcg ctgaaccttc cggtggccaa atcttttccc gcttccccgg
     5161 cggccgtccg ggccgacgcc gg
//
LOCUS       DAOQLS010000055         5166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7366_length_5166_cov_3.607318, whole genome shotgun sequence.
ACCESSION   DAOQLS010000055 DAOQLS010000000
VERSION     DAOQLS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7366_length_5166_cov_3.607318"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PK362_01695"
     CDS             <1..1244
                     /locus_tag="PK362_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73587.1"
                     /translation="RMFRWLEPRLTYNFTGTETNGLPTASSPTAASAKTLDRSGTGDL
                     FVSLAPRDLLPNTKVFRTLNIDNSFRIESADTYENVSNAFLDWRKRQVLRLSKIHRKD
                     GRAMYGLLTPLEFNDPATRRKQLTARNTSRFSANWTPLDWIPAPLRLQPLKTLNLTGT
                     YTNTDEHTENTETLRDVNTVSWPDVLVTLRDTEKFMGLEKWMSGSQINLRANRKRSET
                     FNEELSDSKTNGGDYRLTLFARYDCFLSYSRTDSFVRDLRTGLLKTDTTGLNHSYQVG
                     AKYGSWRVTPSATFRLDHSVDGTGRALQDLDTQTYSVLGRFDRSYPSGFRFPFTKKVF
                     GNVNRLTVDVKTSFERRASSLNFERDNTDTYSGEATGEWEISRNFRLSFGGKTSVVNN
                     RAKPEDGLMTLELNSQLVIQF"
     gene            1247..1966
                     /locus_tag="PK362_01700"
     CDS             1247..1966
                     /locus_tag="PK362_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNC73588.1"
                     /translation="MLGAARRAALEGLGFFFPRTCGVCGRDLPSEADPLCALCVGAFP
                     RWAGFSCRVCGIPLPDGGARCDACRRSRRSFRFCRSGGLYDGPLREALLRLKYGAREA
                     LARPLGRWLALRWRERPELGKVDVVTAVPLHFLRRHARGYNQSERLARSFAAAVGLPY
                     LEVLRRTRWTRPQTRLGRDARKENVAEAFRVALPESVAGKRFLLIDDVCTTGATLDAA
                     AGVLRRAGARWVAALTAARQT"
     gene            complement(2909..3631)
                     /locus_tag="PK362_01705"
     CDS             complement(2909..3631)
                     /locus_tag="PK362_01705"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC73589.1"
                     /translation="MSEPRVVHDQRPAAPPPLSFLVPAFNEIQSIESVLEALNGFPES
                     HEIIVVDDGSTDGTREKLESLRPANTLVLFHAANGGKGAAVRTALARARGRFTAIQDA
                     DLEYDPLDYRELLDVARRDNARAVFGSRFLRANPNIYRRFLWGNRLMTAWINGLTGAR
                     YTDTYTCYKLIESDLFRALALEARGFEMEAEICVKLSARGVFPTEVPIHYKPRRVEEG
                     KKIGWRDFFKGVVAALKHRRPK"
     gene            complement(3713..3795)
                     /locus_tag="PK362_01710"
     tRNA            complement(3713..3795)
                     /locus_tag="PK362_01710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3759..3761),aa:Leu,seq:gag)
     gene            complement(3822..4166)
                     /gene="secG"
                     /locus_tag="PK362_01715"
     CDS             complement(3822..4166)
                     /gene="secG"
                     /locus_tag="PK362_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNC73590.1"
                     /translation="MIYSIVLSIHVVVCVLVILVVLLQSGKGAGFAGLMGGGGGDALF
                     SAPSGSSFIRKVTTGFAVAFFTTSLLLTFYAARRGTRSVTQNLVLPPVAAPTAPEAAP
                     APATPAPASTKK"
     gene            complement(4181..>5166)
                     /locus_tag="PK362_01720"
     CDS             complement(4181..>5166)
                     /locus_tag="PK362_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNC73591.1"
                     /translation="PKYSLAPVAQHLSQLIKKPVAFVADCVGAQAEEAARTLKNGDVL
                     LLENLRFHPEEEKNDPAFAKKLAGLGELFVQDAFGAVHRAHASTAGVPALLPSAAGLL
                     LLKELDYLGRIKENPAKPFVAIVGGAKVSDKIEVLDKLIEKVDSLVIGGAMAYTFLRA
                     QGVATGKSLVEPDKVDLAKDLMKKAAARGAQVLLPLDHVAVKAIDNPASAHDTAGTAI
                     ADDELGVDVGPKSLQSVAGPLAAAKTIFWNGPMGIFEVDRYAAGTRRVAALSADAATK
                     GCVVVVGGGDSVAAVQSTGLADKISHISTGGGASLEFLEGKELPGVTALAR"
BASE COUNT          929 a         1626 c         1685 g          926 t
ORIGIN      
        1 ggcggatgtt ccgttggctg gagccccggc tgacctacaa tttcacgggc acggagacca
       61 acggcctgcc cacggcttcg tcgccgacgg ccgcgtcggc caaaaccctg gaccgctccg
      121 gcacggggga tttgttcgtc tcgctggcgc cccgggacct cctgcccaac accaaggtct
      181 tccgcacctt gaacatcgac aacagttttc ggatcgaaag cgccgacacc tacgagaacg
      241 tctccaacgc cttcctggat tggcgaaaac ggcaggtcct ccgcctgagt aaaatccatc
      301 ggaaagacgg ccgcgcgatg tacggcctcc tgacgccttt ggagttcaac gatccggcca
      361 cccggcgaaa acaattgacc gcccggaaca cctcccggtt ctccgccaac tggactcccc
      421 tggattggat cccggcgccg ctccgcctcc agccgctcaa aacgttgaat ctgacgggca
      481 cctacacgaa caccgacgaa cacacggaaa acaccgagac ccttcgggac gtgaacaccg
      541 tgagctggcc cgacgtcctg gtcaccctgc gggacacgga aaaattcatg gggctggaaa
      601 agtggatgag cggttcccaa atcaacctgc gggccaatcg caaacgatcc gaaaccttca
      661 acgaggaatt gtccgacagc aaaaccaacg gcggcgacta ccggctgacc ctgttcgccc
      721 gttacgactg ctttttgagc tattcccgga cggacagttt cgtccgggac cttcggacgg
      781 gattgctaaa aaccgacacg acggggttga accactccta tcaagtgggg gccaaatacg
      841 gttcctggcg ggtgacgccg agcgccacgt tccggttgga ccattccgtg gacgggacgg
      901 gccgcgccct gcaggatttg gacacccaga cctattccgt gctgggccgc ttcgaccgct
      961 cctatccctc ggggttccgg ttccccttca ccaaaaaagt cttcggcaac gtgaaccgcc
     1021 tcacggtcga cgttaaaacg agttttgagc gccgggcctc gagtctcaac ttcgagcggg
     1081 acaacacgga cacttattcc ggggaggcca ccggcgagtg ggaaatctcc cggaacttcc
     1141 ggctctcctt cggcggaaaa acctccgtcg tcaacaaccg ggcgaaaccc gaggacgggc
     1201 tcatgaccct ggaactcaac agccagctgg tgatccaatt ctaacggtgt tgggcgcggc
     1261 gcgccgggcg gcgttggagg gcctgggatt cttttttccc cggacctgcg gggtgtgcgg
     1321 gcgggatttg ccgtcggagg ccgaccccct ctgcgccctc tgcgtcggcg cgttcccccg
     1381 atgggcgggt ttctcctgca gggtctgcgg aattcccctg ccggacggcg gggcccggtg
     1441 cgacgcgtgc cggcggtccc gtcgctcctt ccggttttgt cgaagcgggg gattgtacga
     1501 cgggcctctg cgcgaagccc tgttgcggct taaatacggc gcccgggagg ccctggcccg
     1561 accgctgggc cgatggctgg cgctgcgttg gcgcgaaagg ccggaattgg ggaaggtgga
     1621 cgttgtgacc gccgtgcctt tgcacttcct ccgccgccac gcccggggct acaaccaatc
     1681 ggaacggttg gcgcggtcct tcgccgccgc cgtgggattg ccgtatctcg aggtcctgcg
     1741 ccgaacccgt tggacgcgac cccaaacccg cctgggccgg gacgcccgga aggaaaacgt
     1801 ggccgaggcc tttcgcgtgg cccttcccga atcggtcgcg gggaagagat ttcttttgat
     1861 cgacgacgtc tgcaccaccg gcgccacctt ggacgccgcc gccggggtcc tccgccgcgc
     1921 gggggcccgc tgggtcgccg ccctcaccgc cgcccgccaa acttaatccg ctctgttttt
     1981 tcctttggat attgtttgcc gttagggggt tcggggcccc ttaaggcgaa tcgagcacac
     2041 cgttttggcg gctgtggtgc ggtccgttgt gccggcgata ggcttttgag ccgagccggt
     2101 cacgttgctg gcggcggagc cgccggtgac cgggccctgg tgtttgggcc acgtgaggac
     2161 cgcccgtcaa aacggtgcgc gcaggccgcc ggggcgactt tcttttggtt acttttcttt
     2221 gggcggccaa agaaaagtaa cccgggtcca gggcggcctg tcgcatcgct ccaggcacct
     2281 ggggcggcag cgccactgat gcggagcgcc tgggactttc gctgttggcg ttttctcttt
     2341 ggaaaaatgc ccgaacaatg agtcgcgggc ctccccggcc cgcccccggg caagggccct
     2401 ttttgggcgc gcaaaaaggg cccctgcacc ccaaaaacgc gccccggtcg gcctgcgcgc
     2461 gatttttcga aggaggccgg accaactccc ggacctcaaa atcccacggt ccggatcgaa
     2521 aggcaccgtc cgacggtttg gattttcacc aaagcctcaa aatcgatttt ttgggtcgga
     2581 cgggacaggt ccggccccgc gggccttcga aaaatcgtgc tcgaatcgcc tcaagggggc
     2641 cccagaaccc aaaaaggcaa atacgaagga caccaacaaa aagggtgaag tttgataggg
     2701 gcggggcggg attgtcgggg gggttgtatt actcgttgcg gctggactta ctgggacttg
     2761 gccgggcctc ggccaccgcg gcggcaaaaa gtttgtgctc aaaccgcctc aagggggccc
     2821 cggaaacact tcacaggaaa tattaaaaac cagcagtggc gggggatttt aaagacggga
     2881 aaaggcgatg ggattacgga tcaataaact acttcgggcg gcggtgtttg agggcggcga
     2941 cgacgccttt gaagaaatcc cgccagccga ttttcttgcc ctcctccaca cggcggggtt
     3001 tgtaatggat cggcacttcc gtcgggaaaa ccccccgggc ggagagcttc acgcaaattt
     3061 cggcttccat ctcgaagccc cgggcttcga gggccagggc ccggaaaagg tccgactcga
     3121 tgagcttgta gcaggtgtag gtgtcggtgt agcgggcgcc ggtcaggccg ttgatccagg
     3181 ccgtcatgag gcgattgccc cagaggaatc ggcggtagat gttggggttg gcgcggagga
     3241 accgggagcc gaaaacggcg cgggcgttgt cgcgccgggc gacgtccagg agttcccggt
     3301 aatccagggg gtcgtattcc aaatcggcgt cctgaatggc ggtgaatcgg ccccgggccc
     3361 gggccagggc cgttcgaacg gccgcccctt tcccgccgtt ggcggcgtgg aaaagcacca
     3421 gcgtgttggc gggacgaagg ctttccagct tctcccgggt gccgtcggtg gagccgtcgt
     3481 ccacgacgat gatctcgtgg gattcgggga agccgttcaa ggcctcgagg accgattcaa
     3541 tgctttggat ttcgttgaag gcgggtacca gaaaagacag cggcgggggc gccgccgggc
     3601 gttgatcgtg aacgacgcgg ggttcgctca ccgaaaggga cacgcgatca aaggccctgg
     3661 ggccgggaaa cgctggggag ggtcgcttcg aacatttgtc ttatgcacac cgtgcgcggg
     3721 gggggacttg aacccccaat gcctttcggc atacgcacct caagcgtacg tgtctgccaa
     3781 tttcaccacc cgcgcacggt gggcacaaga caaaaatcgg tttacttctt ggtcgaagcg
     3841 ggcgccgggg tggccggggc cggggcggct tcgggcgccg tgggcgccgc gacgggcggc
     3901 aacaccaggt tctgggtcac gctgcgggtt ccgcggcggg ccgcgtaaaa ggtcaggagg
     3961 agcgaggtgg tgaaaaaggc caccgcgaaa ccggtcgtga ctttgcggat gaaggaggag
     4021 cccgacggcg ccgagaatag cgcgtcgccc ccgccgccgc ccatgaggcc ggcgaacccc
     4081 gcccctttgc cggactggag caacaccacc aaaatcacca acacgcagac gacgacgtgg
     4141 atcgagagga cgatgctgta aatcatgggg tctcctgggc ctaccgggcc aaggcggtca
     4201 cgcccggaag ttccttgcct tcaaggaatt ccagggaggc gccgccgccc gttgaaatgt
     4261 gcgaaatttt gtcggccagc ccggtggatt gaaccgccgc cacgctgtcg ccgccgccga
     4321 ccaccacgac gcatcccttg gtggccgcgt cggccgacag ggcggccacg cgccgggtgc
     4381 ccgcggcgta acggtccact tcgaaaatgc ccatggggcc gttccagaaa atcgtcttgg
     4441 ccgcggccag ggggccggcg accgattgaa ggctcttggg ccccacgtcc acgcccagct
     4501 cgtcgtccgc gatggcggtg cccgccgtgt cgtgggccga cgccgggttg tcgatggctt
     4561 tgaccgccac gtggtccagg ggcaacaaca cctgggcgcc ccgagcggcg gcttttttca
     4621 tcaggtcctt ggccaggtcc actttgtccg gctccaccag ggattttccc gtggccacgc
     4681 cctgggcccg caaaaaagta taggccatgg ccccgccgat caccagcgag tccacttttt
     4741 cgatcaattt gtccaacacc tcgattttgt ccgacacctt ggcgccgccg acgatggcca
     4801 cgaagggctt cgcggggttt tccttgatgc ggcccaaata gtccaattcc ttgaggagca
     4861 gaaggcccgc cgcgctgggc aggagggccg ggacgccggc cgtgctggcg tgggcccggt
     4921 ggaccgcgcc gaaggcgtcc tgaacgaaga gttcgcccaa ccccgccaat ttcttggcga
     4981 aggccgggtc gttcttttct tcctcggggt gaaaccggag gttttcgagc aacaaaacgt
     5041 cgccgttctt gagcgtgcgg gccgcttcct cggcctgggc cccgacgcaa tcggccacga
     5101 aggccacggg ctttttgatc aactggctga ggtgttgggc caccggggcc aggctgtatt
     5161 tcgggt
//
LOCUS       DAOQLS010000056         5164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7372_length_5164_cov_4.209826, whole genome shotgun sequence.
ACCESSION   DAOQLS010000056 DAOQLS010000000
VERSION     DAOQLS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7372_length_5164_cov_4.209826"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PK362_01725"
     CDS             <1..660
                     /locus_tag="PK362_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73592.1"
                     /translation="LPTAGVWLALVPVFTRLPREWTAWAPLAGGIVGYGLIHFAFRRP
                     ISLYVFGHELTHALAALVSGYKVKSLYVSEKGGEVQLSNTNVVVALAPYCLPIYTMIV
                     VGLYLFVNRYAGFATPPVWFGVGVGATLAFHVALTVHALRQHQPDLHHAGVYFSLVAI
                     LFCNTLTVAALVSLLFPASLRWTEFALESVRRTRGLAEGLWAVSSAGGRRMLEWAREV
                     R"
     gene            657..1244
                     /locus_tag="PK362_01730"
     CDS             657..1244
                     /locus_tag="PK362_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73593.1"
                     /translation="MNVFQHEGARKLFHSLSAGYALLYAAAGRTPTLWALGAALAVSA
                     AVEAIRLRNPRVNQFLLSRFGGIHRDKEIDKPSGIFWTLAGTVGVVALVPARDIVLAS
                     ILYLTVGDGLAGFVGRNWGRFRIGAKSLEGSLAAFLGCWAVGALTLHSPFGRAEVLWG
                     ALGATALEALDLPPDDNFTLPLLSGLGLWAARAFL"
     gene            1241..1996
                     /locus_tag="PK362_01735"
     CDS             1241..1996
                     /locus_tag="PK362_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73594.1"
                     /translation="MIKTEGLLKKFQDVVAVDRLDLEVPAGEIFGLLGPNGAGKTTTV
                     KLLTGLLKPTAGRSWIGGIDVQRDPRGAKKIMGLVPDQPYVYPHLTGKEFLRFVGDLY
                     DVGPRRQIKRIPELLRMFELEDMGDELVESYSHGMRQKLVLAGVLLHEPKVLFLDEPM
                     VGLDPKSARLVKDIFQELSKGGVTIFMCTHVLEIAEKLCHRIGIVEKGRMTRIGTVEE
                     LRSQSQRAGSLEDVFLRLTGGDEYRALLKYLAE"
     gene            1993..3654
                     /locus_tag="PK362_01740"
     CDS             1993..3654
                     /locus_tag="PK362_01740"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73595.1"
                     /translation="MIFLLRIQFLKLKNWLTRSTTGETLKTGGFALLGLLFLVLLYFG
                     FVRVLSALRAVEIIGTLLLLKVLSMAFLTTFFMIVFSSALAAFTTLFFARDLSMLIHT
                     PLPHRTLFFFKSLETAVFSSWMVMVALVPFLGAYGRVTGQGAGFYFLLMGLGIPFAWT
                     ASALGIGLSMTLMSVFPARRVREVMLTLAILIGTGLFLWARWAAPARFVRADNLEVII
                     QYIAQLEAPTAPYLPSWWMSLAVSSYAAGRLGEVLSYGALLGAVAVAAGALLVAFGER
                     AYYSGWAAAQEGGRRRTVTPLGREWRWIPRFFSTRVRALLGKDFAIFRRDSNQWSQLL
                     MLMSIVAVYLISIRKLPLDTPYLRGLVSFLNIGMVGFVLASVALRFVFPEISLEGRAW
                     WVLRAAPMSLWSVLGGKFLSGFLPLSVVGVVLVWVSNAFLHVDPFVVWLSNGTVFVMA
                     GALAGMGVGFGALFPRFNLENIAQIQTSAGGMIYMVAALFYVGLTLSLEAVIMRLYYY
                     SRVAGRSLWSWEPVVLVGAALLVVNLVAVAGPLLMGKAHLERADI"
     gene            3679..4020
                     /locus_tag="PK362_01745"
     CDS             3679..4020
                     /locus_tag="PK362_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73596.1"
                     /translation="MKKWTLTAAALGLGVGLATGASLRSFRNGVYYTPDTVEEKLEFN
                     ESGDNSRFSHFRAAASIPKDYGRLAGVTSGESGATLWFEDAEGNLRNVFVEESRLIHI
                     RRSGTSVARRP"
     gene            4099..>5164
                     /gene="hisD"
                     /locus_tag="PK362_01750"
     CDS             4099..>5164
                     /gene="hisD"
                     /locus_tag="PK362_01750"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNC73597.1"
                     /translation="MKNTVARLLEEVRRRGDKGVASLLRRFDGVSRTPRQWRIPLEEA
                     RRALRRLPAERRRVLETAQRNIARFHAAERRFAGRSWRLRGRGLVVGQEVRPVASAGL
                     YVPGGRFAYPSTVLMTAVPAKAAGVKRVVVVTPPRHMTDEVLGACALAGVDEILSLGG
                     VAAIGALAFGTETIRPVDIIAGPGGAWVTEAKRQVFGHVGIDMLAGPSEIVLLADASV
                     PASLVAADMMAQAEHDPAARAVVISTDSRVLAAVKAAVEPRFRKQCRFLKAADWSAAA
                     RLAEDAAPEHLSLAVKNPEGLLKKIRNAGAVFLGPWSPVAAGDYWAGPSHVLPTGRSA
                     RFSSGLSVQTFLKRSSVIGVS"
BASE COUNT          727 a         1686 c         1652 g         1099 t
ORIGIN      
        1 ctgcccaccg cgggggtgtg gctcgccttg gtccccgttt ttacgcgttt gccccgggag
       61 tggaccgcct gggccccgct ggccggcggc atcgtcggct acgggctgat ccatttcgcc
      121 ttccgccgcc ccatttcgct ctacgtgttc ggccacgagc tgacccacgc cctggcggcc
      181 ctggtctccg gttataaggt gaaatccctt tacgtgtcgg aaaaaggcgg ggaagtccag
      241 ctgtcgaaca ccaacgtggt cgtcgccctc gcgccttatt gccttccgat ttacacgatg
      301 atcgtcgtcg gcctctacct gttcgtcaat cgttacgccg gcttcgcgac gccccccgtg
      361 tggttcggcg tcggtgtcgg cgccaccctg gctttccacg tcgctttgac ggtccacgcc
      421 ctgcgccagc atcagccgga cctccaccac gcgggggtct atttttccct ggtggccatc
      481 cttttctgca acacgctgac ggtggcggcc ctggtgtccc tgctcttccc cgcgtccctg
      541 cggtggacgg aattcgccct ggaaagcgtc cgacgcaccc gggggctggc ggaagggctt
      601 tgggccgtct cgtccgcggg cgggcgccgg atgttggagt gggcccggga ggtccgatga
      661 acgttttcca acacgaaggc gcccgcaaac tgtttcattc cttgagcgcc ggctacgcgc
      721 tcctctacgc cgcggcgggg cggaccccga cgttgtgggc gctgggcgcc gccttggccg
      781 tttcggccgc cgtggaggcg attcgcctgc ggaatccccg ggtcaatcaa ttcctcctga
      841 gccgcttcgg cggcattcac cgggacaagg aaatcgacaa accctccggc atcttttgga
      901 ccctggcggg caccgtcggg gtcgtggccc tggtgccggc ccgggacatc gttctggcct
      961 ccatcctcta tctcaccgtg ggcgacggcc tggccggttt cgtgggacgc aattggggtc
     1021 gtttccgcat cggggccaaa agtctcgaag gcagtttggc cgccttcctg gggtgttggg
     1081 ccgtgggcgc cctgaccctc cattccccct tcggccgcgc cgaagttctc tggggcgccc
     1141 tgggcgccac cgctttggaa gccttggacc ttccgcccga cgataatttc acgcttcccc
     1201 tgctgtccgg cctggggctc tgggcggccc gggcttttct gtgatcaaaa ccgaagggct
     1261 tctgaagaaa tttcaggacg tcgtggccgt ggaccggctc gatctcgagg tcccggccgg
     1321 ggaaattttc gggttactgg gtccgaacgg cgccgggaaa accaccaccg ttaaactgct
     1381 cacgggcctg ctcaagccca cggcgggacg gtcctggatc ggcggcatcg acgttcagcg
     1441 ggacccccgc ggggccaaga aaatcatggg cttggtcccg gaccaaccct acgtctaccc
     1501 ccacctcacc gggaaggaat ttctccggtt cgtcggcgac ctctacgacg tgggcccgcg
     1561 ccgccaaatc aagcggatcc ccgaactgct ccggatgttc gaactggagg acatgggcga
     1621 cgaactggtg gagtcctatt cccacggcat gcggcagaaa ttggtcctgg cgggcgttct
     1681 cctgcacgaa cccaaggtcc tctttttgga cgaacccatg gtcggcctgg accccaaaag
     1741 cgcccggctg gtgaaggaca tttttcaaga gttgtctaaa ggcggcgtga cgattttcat
     1801 gtgcacccac gtcctggaga tcgccgaaaa actctgccac cggatcggca tcgtcgaaaa
     1861 gggacggatg acccggatcg gcaccgtgga ggagctccgt tcccagtccc agcgcgcggg
     1921 gtctctggaa gacgtcttcc tccgtctgac cggcggcgac gaataccgcg cgcttctcaa
     1981 atacctcgcc gagtgatctt cctccttcga atccagtttc tcaaacttaa gaattggctc
     2041 acccgttcca ccacgggcga aaccctgaaa accggcgggt tcgccctgct cggtttactc
     2101 ttcctggtcc tgttgtattt cggtttcgtg cgggtcctgt cggccctgcg ggccgtggag
     2161 atcatcggaa ccctcctgct ccttaaggtt ctgtccatgg ccttcctgac gacgtttttc
     2221 atgatcgtct tttcgagcgc cctggcggcc ttcacgacgc tttttttcgc ccgggacctg
     2281 tccatgctca tccacacgcc cctcccccac cggacgctct tttttttcaa atccctggag
     2341 acggctgttt tttcctcctg gatggtgatg gtggccctcg ttccctttct gggcgcctac
     2401 ggccgggtca cgggccaggg ggccgggttt tatttccttc tgatgggcct ggggatcccc
     2461 ttcgcctgga cggccagcgc cctggggatc ggcctctcca tgacgctcat gagcgtcttt
     2521 cccgcccggc gggtgcggga agtcatgttg accctggcga tcttgatcgg cacggggctt
     2581 ttcctctggg cccggtgggc cgccccggcg cgattcgtcc gggccgacaa cctggaagtg
     2641 atcatccaat acatcgctca attggaggcg ccgaccgccc cctatctgcc ttcctggtgg
     2701 atgtccctgg cggtgtcgag ctacgccgcc ggccgattgg gcgaagtctt gagctacggc
     2761 gcgttgttgg gcgccgtggc cgtggcggcg ggcgccttgc tcgtggcctt cggcgagcgg
     2821 gcctactatt ccggttgggc cgccgcccag gagggcgggc gtcggcgcac ggtcacgccc
     2881 ttggggcggg aatggcgctg gatcccccga tttttcagca cgcgggtacg cgctcttctc
     2941 ggcaaggatt tcgctatttt tcgacgggac tccaaccaat ggtcccagct tttgatgctg
     3001 atgtccattg tggccgtcta tttgatcagc atccgaaaac tgcccttgga cacgccctat
     3061 ttgcgcgggc tcgtttcttt cctgaacatc ggcatggtcg ggttcgtcct ggcgtcggtg
     3121 gctcttcgct tcgtgtttcc cgaaatcagc ctcgagggac gggcctggtg ggtccttcgg
     3181 gcggccccca tgagcctttg gtccgtcctg ggcggcaagt tcctgtcggg gtttctgccc
     3241 ttgagcgtcg tgggcgtcgt cctggtctgg gtgtccaacg ccttcctcca cgtcgacccc
     3301 ttcgtcgtct ggctctccaa cggcacggtg tttgtcatgg cgggggcgtt ggcgggcatg
     3361 ggcgtcgggt tcggcgccct tttcccgcga ttcaacctgg aaaacatcgc ccaaatccaa
     3421 acctccgccg ggggaatgat ttacatggtg gccgccctat tctacgtcgg cctcacgttg
     3481 tcgctcgagg ccgtgatcat gcgcctctat tattattccc gggtggcggg ccggtccctc
     3541 tggtcctggg aaccggtagt tcttgtgggg gcggccctcc tggtcgtgaa cctcgtggcc
     3601 gtggccgggc cgctgttgat gggaaaagcc catttggagc gggcggatat ttagacgacg
     3661 aattcccggg aggtcgcaat gaaaaaatgg acgttgacgg cggcggcgtt gggtttgggc
     3721 gtggggttgg cgacgggggc gtccctgcgg tcctttcgaa acggcgtgta ttacaccccc
     3781 gacaccgtgg aagaaaaact ggagttcaac gaaagcggcg acaactcccg attcagccat
     3841 ttccgcgccg ccgcctcgat ccccaaggat tacggccgtc tggcgggggt gacctccgga
     3901 gagtccggcg ccacgctgtg gtttgaagac gccgagggga accttcggaa cgttttcgtc
     3961 gaagaatccc gattgatcca cattcgccgc tccggaacga gcgtcgcgcg ccggccctga
     4021 tttcccgggg cgccccgcgc ccgattcgac ccgtcttttc cgacggattt acaacacttt
     4081 ttgtataatt tccccttcat gaaaaacacc gtggcccgtc tcctggaaga ggttcgccgc
     4141 cggggggaca agggggtggc gtccctgctc cgccggttcg acggcgtctc ccggaccccc
     4201 cgccaatggc ggattccctt ggaggaggcg cgtcgggctc ttcgacgcct tcccgccgag
     4261 cggcgccggg ttctggaaac ggcccaacgg aacatcgccc gtttccacgc ggcggaacga
     4321 cgctttgcgg gccggtcctg gcgtttacgg ggccggggcc tggtggtggg gcaggaggtc
     4381 cgtcccgtgg cttcggcggg cctttacgtt cccggcggcc ggttcgccta cccgtccacg
     4441 gtgttgatga ccgcggtccc cgccaaagcg gcgggagtga agcgcgtcgt cgtggtcacg
     4501 cccccgcgtc atatgaccga cgaagtgttg ggggcctgcg ccttggccgg cgtggacgag
     4561 attttaagcc tcggcggagt ggccgccatc ggcgccctgg ctttcggcac cgaaaccatc
     4621 cgccccgtgg acatcatcgc cgggccgggc ggcgcctggg tcacggaagc caagcggcag
     4681 gtgttcgggc acgtcggcat cgacatgttg gcgggaccca gtgaaatcgt ccttctcgcc
     4741 gacgcgtcgg ttcccgcgtc tctcgtggcg gccgacatga tggctcaggc ggaacacgac
     4801 cccgcggccc gggcggtcgt gatctccacc gattcccggg tgctggcggc cgtcaaggcg
     4861 gcggtggagc cccgatttcg aaagcagtgc cggttcctca aagccgccga ctggtccgct
     4921 gcggcgcgct tggcggaaga cgcggccccg gaacatttgt ccctggcggt gaaaaacccc
     4981 gaggggctcc tgaaaaaaat ccgaaacgcg ggcgcggtgt tcctgggtcc ctggtcgccg
     5041 gtggcggcgg gggattactg ggccggtccc tcccacgtgc tgcccacggg gcggtcggcc
     5101 cggttttcct cgggcctgtc ggtccaaact tttttgaaac gctccagcgt catcggggtg
     5161 tccc
//
LOCUS       DAOQLS010000057         5146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7410_length_5146_cov_4.138676, whole genome shotgun sequence.
ACCESSION   DAOQLS010000057 DAOQLS010000000
VERSION     DAOQLS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7410_length_5146_cov_4.138676"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="PK362_01755"
     CDS             <1..1446
                     /locus_tag="PK362_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC73598.1"
                     /translation="PTAPRSGRSGGFAEGFLENLSHDFRTPLNGLLGAAALLADSPLA
                     PHQREWLSILRRSAQALNDTVEVLLDHARWQAGDLAPREADFWLKDFLADLMATVKAS
                     LAEITPPLTLEAPSEAVPCRGDADRLRQILRLLILRVLRTGEPGPRVLRVARVDRPGE
                     LAVLRFEFPAEGPADVPSGPEDLPAAGSEKLGFALAQNLLRQLGGTLTAAAGDKGGEA
                     FRLEISYAAPLGAQVEAPKAPLTGSSTTPRPPRAHYRILVAEDNPVNQKVLLLQLEKL
                     GYRADVAADGRTAVELFKKKPYDLVLMDCQMPVLDGYGAVEEIRRWEDKKRRTPVVAV
                     TANATEEDRERCRTAGMDEYLTKPLAVETVAATLARWDVSVEPETLKGLKDLGEGDFP
                     ALREDFLRQTHEQIGALRQAVVGAAWKEIKAAAHKIKGTGGTFGALRLQRLSRWIEDA
                     ADRKDGAEAALLTDSLEEEWGVVAWCLGAGR"
     gene            complement(1443..3599)
                     /locus_tag="PK362_01760"
     CDS             complement(1443..3599)
                     /locus_tag="PK362_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC73599.1"
                     /translation="MATAHQFQDEWIVKIAEPLGLPADVVPGLRRDGESCMASALLRR
                     GIVDWPRLSDAVARAFAVPALDLNASHVEKTALTLLTQPVCEKLHLIPLRITHTTITV
                     AMAHPLDAEALRETRLLAAREVEAVYALPEKIDALVADCYSDDMRVVGLIEKLDVDER
                     LEVLDSSGAPNTPLNAEGPMERFVNDLIAKAVRMEATEIRVDHGDAASEVRFLLDGRL
                     RTILTLPKGLATGPLAARVKVLAGLDPAERLRGQQGRATLRLAGRELGLRVFSRGTPS
                     GERLALRILDEKVAGFALDDLGLRPALAAKMAALLADPRGLFLVSGPPGSGKTSTLYA
                     LLKKAQGSARTVATIENPIEFKLAGVPQIQVNESQGLHWATALRSALRGNPDAVLVGH
                     LADAETAGVALQASAAKRVLAGVHTDHALAALARLVDLGLPPDKIAGATAAALSQRLL
                     RRLCERCRKPLNAERVDPRLAAAIQSAGGPLNVYEAVGCDECNFTGYRGRFALVELVT
                     LAGPVRDALARAAADADLARAAAESKNWETLAVDALWHVGAGDTTLAEAAPYLTEWLS
                     APPAPPTEKTPVPVLAQKKNRRALVTDDDAVIRTVLRRVLEKEGFAVDEAVDGIQALA
                     RVAETPPDLLLLDLDMPEMDGFGVLKLLRRRMGMVNLPVIVLTASDDEANQKLALNLG
                     ADDYILKPVKPPAIAERVNALFRRLKTETSPVAPRP"
     gene            complement(3688..4044)
                     /locus_tag="PK362_01765"
     CDS             complement(3688..4044)
                     /locus_tag="PK362_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC73600.1"
                     /translation="MAKVLVADDSAVLLKVARLVFEKAGHRVESASDGQMALERARTF
                     RPDLIVLDAEMPVLSGLEACRALKADPLTAGAPVHIYTGHDVDGPAAAAFRAAGAAAV
                     HAKPYTPAALLALLAG"
     gene            4174..4659
                     /locus_tag="PK362_01770"
     CDS             4174..4659
                     /locus_tag="PK362_01770"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNC73601.1"
                     /translation="MKKLLALGFVAAMVPSLALAKGGNHPMGGCGLGYVLFGNNDNTP
                     VMQILAATTNGTSANQTFGMTSGTSGCTEDGAVKFVKEVEVYAEINMKDLSRDMVNGQ
                     GEYLSAFARLLGVKAEKQAEFGAFVRSNYGALVPSAATTSVEMLNALADKLAGRTDLL
                     G"
     gene            4720..>5146
                     /locus_tag="PK362_01775"
     CDS             4720..>5146
                     /locus_tag="PK362_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNC73602.1"
                     /translation="MRRSLMFFLFLAPFALRAADTAYLDALRERARANGLADDPRWRA
                     LLHMKKNWRGRWESRVDGKAFFLSPRGKTDPAAELDATLAAFFEPPPADTDTIHPQGL
                     FPARYAYLREALGFDGTRLPEMSSPRLEKWRQNLDIDGIS"
BASE COUNT          873 a         1691 c         1721 g          861 t
ORIGIN      
        1 cccaccgccc cgcgatcggg ccggagcggc ggattcgccg aggggttttt ggaaaacctc
       61 agccacgatt ttcgcacccc gctcaacggt ttgttgggcg cggccgccct gctggccgac
      121 tcgcccctgg ccccgcacca gcgggaatgg ctgtcgattc tccgccggtc ggctcaggcc
      181 ctcaacgaca cggtcgaggt cctgttggac cacgcccgat ggcaggccgg ggatttggcg
      241 ccccgggagg cggatttttg gctcaaggac tttctggccg atttgatggc gacggtcaaa
      301 gcctccctgg cggaaataac cccgcccctg accctggagg ccccgtcgga ggccgttccc
      361 tgccggggcg acgccgaccg acttcgccaa attttgcgcc tgttgatcct gcgcgtcctt
      421 cggaccgggg aaccggggcc ccgcgttctc cgcgtggccc gggtggaccg cccgggggaa
      481 ttggcggttc ttcgattcga atttccggcg gaaggcccgg ccgatgttcc atcgggtccg
      541 gaagatctcc cggccgcggg atccgaaaag ctcgggttcg ccctggccca aaacctcctc
      601 cggcagttgg ggggcacgct gaccgccgcg gcgggcgaca aggggggcga agcctttcgg
      661 ctcgaaattt cctacgcggc gcccctcggc gcccaggtcg aggcccccaa ggcccccctg
      721 accgggtcct ccacgacccc ccgtccgccc cgggcccatt accgaatcct ggtggccgag
      781 gacaaccccg tcaatcaaaa agtgcttctc ctgcaattgg aaaaactagg ttaccgcgcg
      841 gacgtggccg cggacggccg gaccgccgtc gaacttttta agaaaaaacc ctacgacctg
      901 gtgctgatgg actgccaaat gccggtcctg gacggatacg gggcggtcga ggaaattcgc
      961 cgatgggagg acaaaaaacg gcgaacgccg gtggtcgccg tgacggccaa cgccacggag
     1021 gaggaccggg agcggtgccg cacggccggc atggacgaat acctcactaa acccctcgcc
     1081 gtcgaaaccg tggcggcgac gttggctcgg tgggacgtat cggtggagcc cgaaacgctg
     1141 aaggggctca aggatttggg cgaaggggac ttccccgcgc ttcgggagga tttccttcgg
     1201 caaacccacg agcagatcgg cgccctgcgg caggccgtgg tcggcgccgc ttggaaagaa
     1261 atcaaggccg ccgcccacaa aatcaaaggc accggcggca ctttcggggc cctgcgcctg
     1321 cagcggctaa gccggtggat cgaagacgcg gcggaccgga aggacggggc cgaggccgcc
     1381 ctgctgacgg actcgttgga ggaggagtgg ggggtggtgg cctggtgttt gggggcgggc
     1441 cgttagggcc gcggagcgac cgggctggtt tcggttttta gccgccggaa aagggcgttg
     1501 acccgctcgg cgatggcggg cggtttgacc ggtttgagga tgtagtcgtc ggccccgagg
     1561 tttagggcca gcttttgatt ggcctcgtcg tcgctggcgg tcagaacgat gaccggcaga
     1621 ttgaccatgc ccatgcggcg gcgcaacagc ttgagaacgc cgaagccgtc catctccggc
     1681 atgtccaaat ccagaagaag gagatccggc ggggtttcgg ccacccgggc cagggcctgg
     1741 attccgtcca cggcctcgtc cacggcgaag ccctccttct ccaggacccg ccgaagaacg
     1801 gttcgaatca cggcgtcgtc gtcggtcacg agcgctcgcc gatttttctt ttgagccagc
     1861 accggcaccg gcgttttttc ggtcggcggc gcgggggggg cggacagcca ttccgtcaga
     1921 tagggcgccg cttccgccag cgtcgtgtcg cccgcgccga cgtgccagag ggcgtcgacg
     1981 gccagggttt cccaattctt cgattccgct gccgcccggg cgaggtcggc gtccgccgcg
     2041 gcccgggcca gggcgtcccg caccggcccc gcgagggtga ccagctccac caaggcaaag
     2101 cgtccccggt aacccgtgaa attgcactcg tcgcagccga ccgcttcgta gacgttcagc
     2161 ggtccgcccg cgctctggat ggccgccgcc agtcgggggt cgacgcgttc ggcgttcaac
     2221 ggtttccggc accgctcgca cagccgacgc aacaaacgct gggaaagggc ggcggcggtc
     2281 gcgcccgcga ttttatccgg aggcaggccc aggtcgacca atcgggccag ggccgcgaga
     2341 gcgtggtcgg tgtggacccc ggccaacacc cgcttggcgg cggaggcctg aagggccacg
     2401 ccggcggttt cggcgtcggc caagtggccc acaagcaccg cgtcggggtt cccccgcagg
     2461 gccgaccgca gggcggtggc ccagtgaagg ccttgggatt cgttgacttg aatttggggg
     2521 acgcccgcga gcttgaattc gatggggttt tcaatcgtcg ccaccgtccg ggccgacccc
     2581 tgggcttttt ttagcaacgc gtagagcgtg gaggttttgc cggagcccgg cggacccgaa
     2641 accaaaaaga gcccccgggg gtcggcgagc agggccgcca ttttcgccgc cagggccggt
     2701 cgcagaccca aatcgtccaa ggcgaaaccg gccacctttt cgtcgagaat ccgcaacgcc
     2761 aggcgttcgc ccgacggcgt gccgcgggaa aagacgcgga gacccagttc acgcccggcc
     2821 aggcgcaggg tggcccgccc ctgctggccg cgaaggcgtt ccgcgggatc caggcccgcc
     2881 aggactttca cccgggcggc cagcgggccc gtggccagtc ccttgggaag ggtcaaaatc
     2941 gtccgaagcc gcccgtccag cagaaaccgc acttcgctgg cggcgtctcc gtggtcgacg
     3001 cggatttccg tcgcttccat gcgaaccgcc ttggcgatga ggtcgttgac gaaacgttcc
     3061 atgggtcctt cggcgttcag cggcgtgttg ggcgcgcccg aggaatccaa cacttccaac
     3121 cgttcgtcca cgtcgagttt ttcaatcagg ccgaccactc gcatgtcgtc cgaataacag
     3181 tcggccacca aggcgtcgat tttttccggc agggcgtaca ccgcctccac ctcccgggcc
     3241 gcgagcagcc gggtttcccg cagggcctcg gcgtccaggg gatgggccat ggccacggtg
     3301 atcgtcgtgt gggtaatgcg caagggaatc aggtggagct tttcgcagac gggctgggtc
     3361 aggagggtca gggcggtttt ttcgacgtgg gaggcgttca ggtccagggc gggaacggcg
     3421 aaagcccggg cgacggcgtc cgaaagccgg ggccaatcga cgatgccgcg gcgcaggagc
     3481 gcggaggcca tgcaggattc cccgtcgcga cgcaggcccg gcaccacgtc cgccggaagg
     3541 cccaagggct ccgcgatttt aacgatccac tcgtcttgaa attggtgcgc cgttgccaaa
     3601 atcgaactcc ttccccgggg ggcgatgccc tcaacctttc ctgtataccc ccgatcgacg
     3661 atcctttcaa gaccgacggc ggagcgctca gcccgccagg agggcgagca gggccgccgg
     3721 cgtgtacggt ttggcgtgaa ccgccgccgc cccggccgcg cgaaaggcgg ccgccgccgg
     3781 gccgtccacg tcgtggccgg tgtaaatgtg gacgggagcg cccgccgtga ggggatcggc
     3841 tttcaacgcc cggcaggcct ccaggcccga gaggacgggc atttcggcgt ccagaacgat
     3901 caaatccggt cgaaaggttc gggcgcgctc aagggccatt tgcccgtcgg acgcgctttc
     3961 cacccggtgg cccgcttttt cgaaaacgag tcgggcgacc ttgagcagga ccgccgaatc
     4021 gtccgccacg agaactttcg ccataaccca aagatacgcc cgggaagaat ttgccgcaag
     4081 ggtcgatttc ttgaagcgtc cgttgatttg ccggggtttg tgtggtataa acattgaagt
     4141 cactttcaat cgttatttct tccaggagga aacatgaaaa agttgttggc actcggattc
     4201 gttgcggcaa tggtcccctc gctcgcgctc gccaagggcg gcaaccaccc catgggcggc
     4261 tgcggactgg gttacgtcct cttcggcaac aacgacaaca cgccggtcat gcagattttg
     4321 gccgcgacca ccaacggcac ctccgccaac caaaccttcg gcatgacgtc cggcacctcg
     4381 ggctgcaccg aagacggcgc ggtcaagttc gtgaaggaag tcgaggtcta cgccgaaatc
     4441 aacatgaagg acctctcccg cgacatggtg aacggtcagg gcgaatacct ctcggccttc
     4501 gcgcgtctcc tgggcgtcaa agcggaaaag caggccgaat tcggcgcctt cgtgcggtcg
     4561 aactacggcg cgctggttcc ctccgccgcc acgacctcgg ttgagatgct gaacgccctg
     4621 gcggacaagc tcgccgggcg cacggacctc ctcggctaag gcgatccctt aaggtttcaa
     4681 agcggcctcc gcgggatccg cgggggccgc tttttttcca tgcgacgatc cctgatgttt
     4741 tttttgttcc tggcgccctt cgcccttcgg gcggcggaca ccgcctattt ggacgccctg
     4801 cgggagcgcg cccgggccaa cggtttggcc gacgatcccc gctggcgggc cctcctgcac
     4861 atgaaaaaga attggcgggg ccgctgggaa agccgggtgg acgggaaggc ttttttccta
     4921 tcgccccggg gaaaaaccga cccggcggcg gagttggacg ccaccctcgc ggcgtttttt
     4981 gagccgcccc ccgccgacac ggacacgatc cacccccagg ggttattccc cgcccgctac
     5041 gcctacctcc gggaagccct ggggttcgac ggaacccgtc tgcccgaaat gtcgtcgccc
     5101 cgccttgaga aatggcggca aaatctggac atcgacggga tctccc
//
LOCUS       DAOQLS010000058         5142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7419_length_5142_cov_4.596029, whole genome shotgun sequence.
ACCESSION   DAOQLS010000058 DAOQLS010000000
VERSION     DAOQLS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7419_length_5142_cov_4.596029"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PK362_01780"
     CDS             <1..1432
                     /locus_tag="PK362_01780"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNC73603.1"
                     /translation="YVVPFITGPAGSPFSKVGVELTDSRYVVLNMGIMTRMGAVAWKE
                     LGESPHFTRGLHAKADLDEKRRFICHFPQDNTIWSIGSGYGGNALLGKKCLALRIASA
                     LGRREGWLAEHMLILGIQYPNGAIRYVAAAFPSACGKTNLAMMVPPDFLEDQGYKVWT
                     VGDDIAWLRVGADGRLWASNPEAGFFGVVPGTNSHSNPNAVESIRRNTIYTNVARRPD
                     GTPWWEGHDDPAPDQAIDWRGRPWTPESTEKAAHPNSRFTAPASQCPSIAPNWEDPRG
                     VPIDAIIFGGRRARLAPLVTQSFSWNHGVFMGATMASETTAAQQGGTVGVVRRDPMAM
                     LPFCGYNMADYFAHWLEMGKRIPRPPKIFLVNWFRHGADGKFLWPGYGENLRVLLWIL
                     ERAAGGGSATESAIGQLPTLDAVNTQGLHHLGPNAMKELLAVNAGDWEKELADIDGFF
                     AKFGDRLPALLRDERNALAARLQKTR"
     gene            1442..1885
                     /locus_tag="PK362_01785"
     CDS             1442..1885
                     /locus_tag="PK362_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNC73604.1"
                     /translation="MTTATAQLIKDHKMIRKLLAELAEGHPRFPQLLATTERVVLGHA
                     WFEDEFLLPALKARPGLFQPFREEIVQEHRDIAGLFLRLRGATPSDPVVRPHLLTLRV
                     LLETHFAKEEDALFPLAEKIIGEEGLVGMAVDMERRKSEVRLPPA"
     gene            complement(1919..2308)
                     /locus_tag="PK362_01790"
     CDS             complement(1919..2308)
                     /locus_tag="PK362_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNC73605.1"
                     /translation="MRGSDSAEVFVSAARAFSALLADPDGVSPGGLHDRLRVEATTRE
                     GLLAAFLNELGRLFEREREIFCRFQPRRVTDTLCEIEADGELWDPGRHRAGHTFKPLS
                     AADVTWTAAPGVPPIVRLCLSVQSPPS"
     gene            complement(2358..4853)
                     /gene="gyrA"
                     /locus_tag="PK362_01795"
     CDS             complement(2358..4853)
                     /gene="gyrA"
                     /locus_tag="PK362_01795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNC73606.1"
                     /translation="MADTKNTEQGDLLDRVIPRAIEEEMKTSYIDYAMSVIVGRALPD
                     VRDGLKPVHRRILYTMDEMGLAHNKPYKKSARVVGDVMGKYHPHGDSAIYDAVVRMVQ
                     DFSLRHPLVDGQGNFGSIDADPPAAMRYTEVRLAAIAQEMLGDIDKNTVDFGPNYDGS
                     LTEPLVLPAKLPNLLINGSAGIAVGMATNIPPHNLVEVCDAVNAYIDNENITTGELLK
                     IIKGPDFPTAATITGREGIKQYFETGRGSITTRAKAEIEDIRGGKQAIIINELPYQVN
                     KAQLLETIAELVRDKKVDGISDIRDESDRDGIRVVVELKRDGNAQVILNQLYKYTQME
                     TSMGVIMLALVNGRPRVLSMREMLKYYVEHRREVVIRRTRHELKRAEDRAHILEGLRI
                     AIDHLDKVIKTIRESKNTEAARTALMEKFELSRIQAQAILDMRLHQLTALERKALEEE
                     YLELIKTIARLKALLADARKILGVIQEELKELKDKYGEKRRTQITAAVEEMEIEDLIA
                     QEDVVVTFSHAGYVKRLPVDTYRAQKRGGRGVTGMTTKEEDFVEQLFVTNTHAHLLLF
                     TTRGRAYSVRVYEIPEAGRASRGKAVVNLVALNPDEKITSAIPVTSFEPEKTKDMGLI
                     LCTRNGQIKRTALSEYDSVRKSGIIAIGLDEGDILVDAKLTDEKREVLIGTKHGMCIR
                     FPAEQVRLMGRGAGGVRGVRLEKDDQVVGMEVTAPARKETLVTACEFGYGKRTELSEY
                     RDQNRGGSGVITIKTSDRNGPVVGIKLVTNDDDLMLMTEKGMTVRVHAKDLSVIGRNT
                     QGYRLIRMEEGDKLANIAPVVAEEEDNGDAKDA"
     gene            complement(4869..>5142)
                     /locus_tag="PK362_01800"
     CDS             complement(4869..>5142)
                     /locus_tag="PK362_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004692939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNC73607.1"
                     /translation="VASIAELLEAIKDAGRKGATIQRYKGLGEMNPQQLWETTMDPQN
                     RKLLRVKLDANSTEADDVFTVLMGDKVEPRRQFIESHASEVQNLDI"
BASE COUNT          840 a         1551 c         1654 g         1097 t
ORIGIN      
        1 gtacgtggtg cctttcatca cgggccccgc cggatcgccc ttttccaaag tgggcgttga
       61 attaaccgac agccgttacg tggttttaaa catggggatc atgacgcgca tgggcgccgt
      121 ggcgtggaag gaactggggg aatccccgca tttcacccgg ggcctccacg ccaaagccga
      181 cctggacgaa aaacgtcggt ttatttgcca ctttccccag gacaacacca tttggagcat
      241 cggctcgggc tacggcggga acgccctttt gggcaagaaa tgcctcgccc tgcgcatcgc
      301 cagcgctctc ggccgccggg aaggctggct cgcggagcac atgctgattt tgggcattca
      361 atacccgaac ggagcgatcc gctacgtcgc ggcggccttc ccctcggcct gcgggaaaac
      421 caacttggcc atgatggtcc cgccggattt tctggaagac cagggataca aagtctggac
      481 cgtgggcgac gacatcgctt ggttgcgggt gggggcggac ggccgattgt gggcctccaa
      541 tcccgaggcc gggtttttcg gcgtcgtccc gggcaccaat tcccactcga accccaacgc
      601 ggtggaaagc atccgccgca acacgattta caccaacgtc gcccgtcggc ccgatggaac
      661 gccttggtgg gaaggccacg acgatccggc gccggaccag gccattgatt ggcgggggcg
      721 cccctggaca cccgaatcca cggaaaaagc ggcccacccc aacagccggt ttaccgcccc
      781 ggcgtcccaa tgcccgtcca tcgcgcccaa ttgggaagac ccccggggcg ttcccattga
      841 cgccattatt ttcggtggcc gccgggcgcg tctggccccg ctggtgaccc agagcttttc
      901 ctggaaccac ggcgtgttca tgggcgccac catggcgtcg gaaaccaccg cggcccaaca
      961 aggcggcacg gtgggcgtgg tgcggcgtga tcccatggcc atgcttcctt tttgcggtta
     1021 caacatggcg gactatttcg cccactggct ggagatggga aaacggattc cccgtccgcc
     1081 caagatcttt ttggtcaatt ggttccgcca cggagccgac ggaaagtttt tgtggccggg
     1141 gtacggggaa aatctgcggg ttctgctgtg gattctcgaa cgggccgcgg gcggcggttc
     1201 ggcgacggag tccgccatcg gtcagttgcc gaccttggac gcggtcaaca cccaggggct
     1261 ccatcacctg ggtccgaacg ccatgaagga gcttttggcc gtcaacgccg gcgattggga
     1321 aaaagagctt gcggacatcg acggtttctt cgcgaagttc ggcgaccgcc ttccggcgct
     1381 gctccgggac gagcggaacg ccctcgccgc ccggttgcag aaaacccgtt aagccccgcg
     1441 cgtgaccacc gccaccgctc agctgatcaa ggatcataaa atgatccgca agttgttggc
     1501 cgaattggcg gaaggccatc cccggtttcc ccaactcctg gcgacgaccg aacgggtggt
     1561 cctgggccac gcgtggtttg aagacgagtt tcttttaccg gcgctgaaag cccggccggg
     1621 gctgtttcaa ccctttcggg aggaaatcgt ccaggagcac cgggacatcg ccggactttt
     1681 cctccgtctc cgcggggcaa cgccctcgga tcccgtggtc cgtccacatc ttctcaccct
     1741 tcgagttctt ctggaaaccc atttcgccaa ggaggaagac gcgttgtttc ccttggcgga
     1801 gaaaatcatc ggggaagagg ggctggtggg aatggcggtc gacatggaac ggcgcaaatc
     1861 cgaggtgcgg ctgcccccgg cctaagggtc cggggctttt tcggatattg ggcgggattt
     1921 aagaaggggg cgattgaacc gaaaggcaaa gccggacgat ggggggaacg cccggcgcgg
     1981 cggtccaagt cacgtcggcg gccgagagcg gtttgaacgt gtgccccgcc cggtgacgcc
     2041 cggggtccca gagttccccg tcggcttcga tttcacaaag ggtgtcggtc acccgacggg
     2101 gctgaaaccg gcaaaagatt tcccgctccc gctcaaacaa acgacccagt tcgttgagga
     2161 aggcggccaa caacccttcc cgggtggtcg cctcgacccg aaggcgatcg tgaaggcccc
     2221 ccggcgaaac gccgtcggga tcggccagga gcgccgaaaa cgcccgggcg gcgctgacga
     2281 aaacctccgc cgaatccgat ccgcgaattg tgagggtcgg ccgcccggtc ccggggtccc
     2341 gatggaccgg cgggtcctca ggcgtccttg gcgtccccgt tgtcctcttc ctccgccacc
     2401 accggcgcga tgttggcgag tttgtcccct tcttccattc gaatcaatcg atagccctgg
     2461 gtgttccggc cgatgaccga caggtctttg gcgtgaacgc ggacggtcat gcctttttcc
     2521 gtcatgagca tgaggtcgtc gtcgttcgtc acgagcttga tgccgaccac gggcccgttg
     2581 cggtccgagg ttttaatggt gatcacgccc gatcccccgc ggttttgatc ccggtattcg
     2641 gaaagttccg tccgtttgcc gtagccgaat tcgcaggcgg tgacgagggt ttccttccgg
     2701 gcgggggccg tcacttccat gcccaccacc tggtcgtctt tttccaaccg aacgccgcgg
     2761 acgccgccgg ccccccggcc catcaggcgg acctgttccg ccgggaaccg gatgcacatg
     2821 ccgtgcttgg tgccgatcag cacttcgcgt ttctcgtcgg tcaatttagc gtcgaccagg
     2881 atgtcccctt cgtccaaacc gatggcgatg atcccgcttt tacgcacgct gtcgtattcc
     2941 gagagcgcgg tgcgtttgat ctgcccgttc cgggtgcaga ggatgaggcc catgtctttg
     3001 gttttctccg gctcgaagga cgtcaccgga atggccgacg ttattttctc gtcggggttg
     3061 agcgcgacga ggttgacgac cgccttgccg cgggacgccc ggcccgcctc ggggatttcg
     3121 tagacgcgga cgctgtaggc ccggccccgg gtggtgaaga ggagcaggtg ggcgtgggtg
     3181 ttggtcacga acagctgttc cacgaaatct tcctctttcg tggtcatgcc ggtcacgccc
     3241 cggccgccgc gcttttgggc gcggtaggtg tccacgggca ggcgtttcac gtagcccgcg
     3301 tgggagaagg tgaccaccac gtcttcctgg gcgatcagat cctcgatttc catttcctcc
     3361 acggcggcgg tgatttgcgt gcgacgcttc tctccgtatt tgtctttcaa ttccttgagt
     3421 tcttcctgga tgacgcccaa gatcttccgg gcgtcggcca acagcgcttt gaggcgcgcg
     3481 atggtcttga tcagctccag atattcctct tccagggcct tccgttcgag agccgttaat
     3541 tgatggaggc gcatgtccaa aatggcctgg gcctggatgc gcgacagttc gaatttttcc
     3601 atgagggccg ttcgggcggc ttcggtgttt ttggattccc gaatggtctt gatcaccttg
     3661 tccaggtggt cgatggcgat gcgcaaccct tccaggatgt gggcgcgatc ctcggcccgt
     3721 tttaattcgt gccgggtccg gcggatgacg acttcccggc ggtgctcgac gtaatacttg
     3781 agcatctccc gcatggagag gacccgggga cgcccgttca cgagcgcgag catgatgacg
     3841 cccatggagg tttccatctg ggtgtacttg taaagctggt tcaggatgac ctgggcgttt
     3901 ccgtcgcgtt tcagttcgac gacgacccgg atgccgtccc gatcggattc gtcgcggatg
     3961 tcggaaatgc cgtcgacttt cttgtcccgc acgagctccg cgatggtttc caggagttgg
     4021 gccttgttga cctgataagg cagctcgttg atgatgatgg cctgtttgcc gccccgaatg
     4081 tcttcgatct ccgctttggc ccgggtggtg atggaaccgc ggccggtttc gaaatactgt
     4141 ttgatgccct cgcgccccgt gatggtggcg gccgtgggga aatcgggacc cttgatgatt
     4201 ttgagcagtt cgcccgtggt gatattttcg ttgtcgatgt aggcgttcac cgcgtcgcac
     4261 acttccacca ggttgtgggg cggaatgttg gtcgccatgc cgaccgcgat gccggcggat
     4321 ccgttgatga gcaggttggg aagcttggcg ggcaggacca ggggttccgt gagggacccg
     4381 tcgtagttcg ggccgaaatc caccgtgttt ttgtcgatgt cgccgagcat ttcctgggcg
     4441 atggccgcga ggcgcacttc ggtgtaccgc atggcggcgg gagggtcggc gtcgatggag
     4501 ccgaagttgc cctggccgtc caccagcgga tggcgaaggg agaaatcctg gaccatgcgg
     4561 accacggcgt cgtagatggc gctgtcgccg tgggggtggt atttgcccat gacgtcgccg
     4621 accacgcggg cgctcttttt gtagggcttg ttgtgggcca agcccatttc gtccatggtg
     4681 tagagaatcc ggcggtgaac cggcttcaag ccgtcccgca cgtcgggcag ggcgcggccc
     4741 acgatgacgc tcatggcgta atcgatgtag gaggttttca tctcctcctc gatggcgcgg
     4801 gggatcacgc ggtccagcag gtcgccttgt tcggtgtttt tggtatcggc catgtcgtgg
     4861 tctccgaatt aaatgtcgag gttctgaact tcggacgcgt gggactcgat gaactgccgg
     4921 cggggctcca ccttgtcgcc catcagcacg gtgaacacgt cgtccgcttc ggtgctgttg
     4981 gcgtccagct tgacgcgcaa gagttttcgg ttttgcgggt ccatggtggt ttcccacaat
     5041 tgctgcgggt tcatttcgcc caaccctttg tagcgctgga tcgtggcgcc tttccgcccg
     5101 gcgtctttga tggcttccag cagctcggcg atggacgcga cg
//
LOCUS       DAOQLS010000059         5141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7425_length_5141_cov_4.777232, whole genome shotgun sequence.
ACCESSION   DAOQLS010000059 DAOQLS010000000
VERSION     DAOQLS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_5141_cov_4.777232"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1591)
                     /gene="radA"
                     /locus_tag="PK362_01805"
     CDS             complement(212..1591)
                     /gene="radA"
                     /locus_tag="PK362_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNC73608.1"
                     /translation="MKTKTRVLFVCRDCGGDTPKWAGQCPACGQWNTLVQRSETPAPA
                     AAPRRLTDFSSKVMSLADTAVLTAERRKTGIGEFDRILGGGLWPGSLVLLGGAPGIGK
                     STLMLQVANRLARPDFPVLYVSGEESPDQVKGRAARLGVRNPSLFLVAETNLTKILEA
                     VEEVRPGVLVVDSVQTVHKPELPGAPGAVAQIRECAAELLHLAKGRGIGVFLLGHVTK
                     EGDLAGPRVLEHMVDTVLYFETERQDIHRILRAVKNRFGPTNEIGVFEMTGAGLAEVQ
                     NPSALFLGDHAGAAPAGTAVLAALEGTRPLLVEVQALTSRTLFGMPRRQMSGVDYNRT
                     SLLVAVLDKRCGFHLDSQDVYVNATGGLEVREPAADLAISAAIASSFLDRSIPPKTLW
                     LGEVGLSGELRPVAQTAERLSEAAQLGFARVIMPKARGRAGEWPKGLEVRAVATLSEA
                     LAQAGLSNQ"
     gene            complement(1626..2147)
                     /locus_tag="PK362_01810"
     CDS             complement(1626..2147)
                     /locus_tag="PK362_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNC73609.1"
                     /translation="MLHEARIYSLANVGGQCVLVLEETEGPRLLPVWIGLYEGGSIGM
                     ALAGQKFPRPLTHDLFLDALERLGTTLEKVVVTDLRDSTFFAEIHLKRDDERLVVDAR
                     PSDSIALAVRRNCPIYVADEVFEACPELLKPISEEEVQEFKKSLQTMKPEDFFKDLKK
                     PPDPGAGGEGPKA"
     gene            complement(2159..3526)
                     /locus_tag="PK362_01815"
     CDS             complement(2159..3526)
                     /locus_tag="PK362_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC73610.1"
                     /translation="MALTSESKVGLFTLAGLFVFAAGILILGDFHIRSRYPLYVYFDD
                     AGGLPEKGPVKIAGVEVGQVDTIGLEGPRARVRIQLREDVAVHKGARAHVSSTGLIGS
                     KFLDLSLGDPAAPVLQPGETLEGDPSLTFDEVMTKLGEFLKEDPKTGAVAENLKTTIA
                     NFRKVSQALADSLGEQRAEMTEIVQNIRDVSAHAKRVAADLAEITTDRKEDIKVALAK
                     FRSVSERLDDLTARVQNGQGLLGKLVTDEKMGDELKQTMTSVKQATKDLETFTGRVSR
                     IEVYWDYHQRYDFEDEKFRADLGLRWVPRPGKFYYIAGNNLGRREDRKADPTADLERR
                     NTVTAVMGKDFGPLTLYAGAIRSAGGGGARFRPLPASSAWNRRVEVEGEAYNFGRDET
                     IRGVKMDKPVYNAGLRVNAIAPWVWVGGGVEDLAVRKNFNANVNVLFKDEDIAFLFGL
                     VNIAR"
     gene            complement(3526..4290)
                     /locus_tag="PK362_01820"
     CDS             complement(3526..4290)
                     /locus_tag="PK362_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73611.1"
                     /translation="MIQLENVHKAFGANRVLRGLSLEIRDGETLTIIGGSGTGKSVTL
                     KLMVGLLRPDQGRVIVDGQDIAGIGEDALAEVQKKFGFLFQGAALFDSLTVAENVLFG
                     VRNLNRKSLGRPDELVERSLGLVGLKPEVARLKPAELSGGMKKRVGLARAIAHEPEYI
                     LYDEPTTGLDPIMSDVINDLIVNIREKLKITSIAVTHDMKSAYKISNRIAMIYEGRLV
                     SVGTPAEIQATTDPVLRQFITGSSHGPIQMPVKAYV"
     gene            complement(4290..5129)
                     /locus_tag="PK362_01825"
     CDS             complement(4290..5129)
                     /locus_tag="PK362_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC73612.1"
                     /translation="MRDLQTGSPPPPGVRVKPIRVTFQLFSGQVVDLSETVGSVVVLL
                     RKTVRSIFTTRLDTKNVVAQMYEIGYQSFAVTSLTALFTGMVLALQTGFSFKKVFNEP
                     LYVGTVVGLSLLKELGPVLTAVVVAGRVGAAIAAEIGTMNVTEQVDALFTLGTNPVRY
                     LVVPRFLACLLMVPILTVFADLIGIGGGYLVAHFRLGIPSSTFWDEINAIELEDGFHG
                     LIKSLAYALIIVTTSCYKGLKTSGGAEGVGRATTSAVVVSMVFILVSDYFLSALLVSL
                     GIG"
BASE COUNT          972 a         1659 c         1573 g          937 t
ORIGIN      
        1 gcgccgatca ccgccgagat caacgcgtcc aacgccaccc gttcgatgac aatttcggcg
       61 ccgatgacca acaaggcgac gccgacgccg atcagaattc ccttggaatc atgggaaatc
      121 tgtgtgtagc tgatgatggg cccgatcacg atgaccaagc cccttatgat gttgataagc
      181 acggagtacc cccccgatta ggataataaa atcattgatt cgaaagaccg gcctgggcca
      241 aggcttccga caaggtcgcc acggcgcgca cctccagccc tttgggccac tcgccggcgc
      301 gaccccgcgc cttgggcatg atcacccggg cgaaacccaa ctgggcggcc tcgctcagcc
      361 gctccgccgt ttgggccacg ggccgcagtt cgccgctcaa tcccacttcc cccagccaca
      421 gggttttggg cggaatggaa cggtccaaaa acgagctggc gatggccgcc gaaatggcca
      481 aatcggcggc gggttcgcgc acctccaggc cgccggtggc gttcacgtag acgtcctggg
      541 aatccaaatg aaaaccgcat cgtttgtcca acaccgccac caaaagcgac gtccggttgt
      601 aatccacgcc ggacatctgg cggcggggca tgccgaaaag ggttcgggag gtgagggcct
      661 ggacctccac caaaagcggg cgggtgcctt ccagggcggc cagaaccgcg gtgcccgccg
      721 gcgccgcccc ggcgtggtcc cccaggaaaa gggccgaggg gttctggact tcggccaacc
      781 ccgcgcccgt catttcgaaa acgccgatct cgttggtggg gccgaagcga tttttgacgg
      841 cccgcaatat tcggtggatg tcctgccgtt cggtttcaaa atacaaaacc gtgtccacca
      901 tgtgctccaa cacccggggg ccggccaaat ccccttcctt ggtcacgtgc cccaggagga
      961 aaacgccgat cccccggccc ttggccaggt ggagcagttc cgccgcgcat tcccgaatct
     1021 gggccacggc ccccggggcg ccgggcaatt ccggcttgtg gaccgtctgc acggagtcca
     1081 ccaccaacac gccggggcgg acctcctcca cggcttccaa aatcttggtc aggttcgttt
     1141 cggcgaccag gaagagggac ggattgcgca cgcccagccg ggccgcccgg cctttcacct
     1201 ggtccgggga ttcctctccc gaaacgtaga gcaccggaaa atcgggacgg gccaatcgat
     1261 tcgccacttg gagcatcagg gttgatttcc cgatgccggg ggcgccgccc aacaggacca
     1321 gggagcccgg ccaaaggccg ccgcccagaa tccggtcgaa ttctccgatg ccggttttcc
     1381 gccgctcggc cgtcagcacc gccgtgtcgg ccagggacat caccttggac gaaaaatccg
     1441 tgagccgccg gggcgccgcc gcgggggccg gcgtttcgga ccgctgaacc aacgtgttcc
     1501 actgcccgca ggccggacac tgccccgccc atttcggcgt gtcgcccccg cagtcccggc
     1561 agacgaaaag gacccgtgtt ttggttttca cggacgggtg gccgacgggg cggaggccgg
     1621 gcggctcatg ctttcggacc ttcgccgccg gctcccgggt cgggcggttt tttcaaatcc
     1681 ttgaagaaat cttccggctt catggtctgc aggcttttct tgaattcctg gacttcctct
     1741 tcggaaatgg gtttcagaag ttccggacag gcctcgaaca cttcgtccgc cacgtaaatg
     1801 gggcaattgc ggcggaccgc cagggcgatg gagtccgacg ggcgggcgtc cacgacgagg
     1861 cgttcgtcgt cccgcttcaa atggatttcg gcgaaaaacg tgctgtcccg cagatcggtg
     1921 acgaccacct tttcgagggt cgtccccagc cgctccaaag cgtccaggaa aagatcgtgg
     1981 gtcaggggcc gcgggaattt ctgacccgcg agggccatgc cgatggaccc gccttcgtac
     2041 agcccgatcc acacgggaag gagccggggg ccttcggttt cctccaacac caacacgcat
     2101 tggccgccca cgttggccaa ggaataaata cgggcttcgt gcagcatggt cgcctttctt
     2161 accgcgcgat attcacgagg ccgaagagga acgcgatgtc ttcgtcctta aagagaacgt
     2221 tcacgttggc gttgaagttc ttgcggacgg ccaaatcctc cacgccgccg ccgacccaca
     2281 cccacggggc gatcgcgttc acccgcagtc cggcgttgta caccggtttg tccattttga
     2341 cgccgcggat tgtttcgtcc cggccgaaat tgtacgcctc gccctcgacc tccacgcggc
     2401 gattccaggc cgaggaggcc ggcaggggac ggaagcgggc gccaccgccc cccgccgatc
     2461 ggatggcgcc ggcgtaaagg gtcagcggac cgaaatcctt gcccatgacg gcggtcaccg
     2521 tgttgcggcg ctccaaatcg gcggtgggat cggctttccg gtcctcccga cgccccaggt
     2581 tgttgcccgc gatgtaataa aacttgcccg ggcgcggaac ccaccgcaac ccgagatcgg
     2641 cccggaattt ttcgtcttca aaatcgtagc gctgatggta atcccaatac acctcgatgc
     2701 gggacacccg cccggtaaaa gtttccaaat ccttcgtcgc ctgcttgacg ctggtcatgg
     2761 tctgcttcag ttcgtcgccc atcttttcgt cggtcaccag tttgcccagg aggccctgtc
     2821 cgttttgcac ccgggcggtc aaatcgtcca gccgctcgga aacggaccgg aatttggcca
     2881 gggccacttt gatgtcctcc ttgcggtcgg tggtgatctc ggccaggtcc gccgccaccc
     2941 gtttggcgtg ggccgacacg tcccgaatgt tttggacgat ttccgtcatc tcggcccgct
     3001 gttcgcccag ggaatccgcc agcgcctggg acactttccg gaaattcgcg atggtggttt
     3061 tgaggttttc ggccacggcg ccggtcttgg gatcttcctt gaggaattcg cccaatttgg
     3121 tcatcacctc gtcgaacgtc agggacgggt cgccctccag cgtttcgccg ggctgaagca
     3181 cgggggcggc ggggtcgccg aggctgagat cgaggaattt gcttccgatc aacccggtgg
     3241 acgacacgtg ggcccgggcg cccttgtgga cggccacgtc ctcgcgaagt tgaatgcgca
     3301 cccgggcccg gggcccttcg agaccgatgg tgtccacctg gcccacttcg acgccggcga
     3361 ttttcacggg gcctttttcc ggcaggccgc ccgcgtcgtc aaaataaacg tacagggggt
     3421 agcggcttcg aatgtggaaa tccccgagga tcaagatccc cgcggcgaag acgaacaacc
     3481 ccgccagggt gaacagcccg actttggatt ccgaggtcag ggccatcaga cgtaggcctt
     3541 gaccggcatc tggatgggcc cgtgggagga ccccgtgatg aactggcgca gcacgggatc
     3601 ggtcgtcgcc tggatttccg cgggggtgcc cacggacacc aggcgccctt cgtaaatcat
     3661 ggcgatgcgg ttggaaatct tataggcgga tttcatgtcg tgcgtcacgg cgatggaggt
     3721 gattttaagt ttctcccgaa tattgacgat caaatcgttg atgacgtccg acatgatggg
     3781 gtccaacccc gtggtcggct cgtcgtacaa aatgtattcc ggctcgtggg cgatggcccg
     3841 ggccaagccc acgcgctttt tcatcccgcc cgacagctcc gcgggtttca gccgcgccac
     3901 ctcgggttta agccccacca accccaggct ccgctccacc agttcgtccg gtcggcccag
     3961 ggacttgcgg ttcaggtttc ggaccccgaa aagtacattt tcggccacgg tgagcgaatc
     4021 gaacagggcc gcgccctgga aaagaaagcc gaactttttc tgcacctcgg ccaaggcgtc
     4081 ctcgccgatc ccggcgatgt cctggccgtc cacgatcacg cgcccctggt ccgggcgcaa
     4141 caggcccacc atgagcttga gcgtcacgct tttgccggtg cccgagcccc cgatgatggt
     4201 cagggtctcg ccgtcccgaa tctccaggga aagtccccgc aaaacgcggt tggcgccgaa
     4261 cgccttgtgg acgttttcca gctggatcat cagccgatgc ccagggacac caggagcgcc
     4321 gacaggaaat aatcgctgac caagataaac accatgctga ccacgaccgc gctggtcgtg
     4381 gcccggccca ccccttcggc gccgccggag gtcttgagcc ctttgtaaca ggacgtcgtg
     4441 actattatga gagcataagc cagggacttg atcaacccgt gaaaaccgtc ctccaattcg
     4501 atggcgttga tttcgtccca aaaggtggaa gagggaattc ccagacggaa atgggcgacc
     4561 aggtacccgc ccccgatgcc gatcaaatcg gcgaagacgg tcaaaatcgg aaccattaaa
     4621 aggcaggcca aaaagcgggg caccaccaga tatcgcacgg gattggtgcc cagcgtgaac
     4681 agggcgtcca cctgttcggt gacgttcatg gtgccgatct cggcggcgat ggcggccccc
     4741 acccggccgg cgaccacgac ggcggtcagg acgggcccca gttcctttaa gagcgacaac
     4801 cccacgacgg tgcccacgta gaggggctcg ttgaacacct tcttgaaaga gaatcccgtt
     4861 tgaagggcga gcaccatccc cgtgaagagc gccgtgaggc tggtgaccgc gaaggattgg
     4921 tagccgattt cgtacatctg ggccacgacg tttttggtgt ccaggcgggt ggtgaaaatg
     4981 gagcggacgg ttttccgcaa cagaacgacg acgctcccca ccgtctcgga gaggtccacc
     5041 acctggccgg aaaaaagctg gaaggtgacg cggatcggct tcacgcggac gcccggtggt
     5101 ggcggggaac ccgtttggag atcccgcaca ggatctcgta g
//
LOCUS       DAOQLS010000060         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7447_length_5134_cov_3.506990, whole genome shotgun sequence.
ACCESSION   DAOQLS010000060 DAOQLS010000000
VERSION     DAOQLS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_5134_cov_3.506990"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2152
                     /locus_tag="PK362_01830"
     CDS             <1..2152
                     /locus_tag="PK362_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNC73613.1"
                     /translation="EWAKFIAGSTYTFTADGLPVDSKTYELVLYARNKNGIETAGSLP
                     LTVALQSGYPEVSLTMPTGEVFVATNSYAVPIYTNETLLKFRALGSGHFNARVTEDVA
                     ISTPEALNLLSPNVYKLWTGWNGTILPFPAGVTATNSFPDPFYADPWSEFDFQTEGLW
                     FFNIAGTLSDLGTPANTYISTSPFRVFIDTTPVDPGTIRAEFGQDATGTPDHTEILPG
                     LPTGHQKPEFFWTKTDTQGKPNPRSPIVGWSWSFSSNPVDTPVLSSAQPQFLPASLFV
                     AASSASYRVFMATYIANFDLVFESTFYFKVAALDKAGNWTPIPSEFQYNYVKDQVPPD
                     LLGFDFPGVEVWTSSPTRYEAVNSTVPVQVIFSELMNFVGGDQAMSIVQTQDHLGNPM
                     GTTIPYTVAWVELPTGTSAVLTPSLPGNQWPTGSRFVLHIDGFFTRDLAGNRLNVNYD
                     VLFNTAMDPAIVNIGKSEDGNTTVTFQPGAFGPNPAGAAIADNPATRPVSAAPGLAGT
                     VRKAVETIVKTKGGDYRKILSIKQFELYDLNRQALKTPFNGQVLLTFQYNDVNDNGIV
                     DETEAKFPVKVKDLAIHWLDEKSGAWIKLPESNVDTAAKTVSIPLQHFSVYALIGAPS
                     LDLSNAHPFPVPYKASTDPAGITFTGLSSIATVKIFTLDGRLVKTLTDDSGAGFVRWD
                     PVNNESGDPVASDVYLYVIENDQQRRIGKLMVIR"
     gene            2149..4113
                     /locus_tag="PK362_01835"
     CDS             2149..4113
                     /locus_tag="PK362_01835"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC73614.1"
                     /translation="MNRRHLWTGLGAFLLGVGSLWAKASGSSTANFLKLGVGGRGVAM
                     GEAHTAAVDDVMSLYWNPAGLGYLYLNEVGFMHNNFVQGISQDVFYYARPTETKGTFG
                     AGVSMLRINDIAGFDASDIPTGNVQASDTMLTFGWGKSMEQLVWFRGMRVGANLKILR
                     KTLDRTSALGYMTDLGLLYETRGGWFHGLNTGFVIQNLGTGLSMGGQKSNFPLQMKLG
                     FAYPMFGDNLVLATDLVLPTDGSLYINMGTDYRIWDILAFRLGYRGSNDLDSGLTYGV
                     RLGNERLHMDYAFVPFGPFGDSHRVSLGVRFGKAFREGKVADQIQTAYMRAEAKYGQG
                     LLVDAYMQATQILDVAPWHRASKLLTKRIESELKAMETGARRQQLQAQVDEHYARGEQ
                     LFQIDDLLHAKREFEAILALQADHMGAKTYLNRIEERFKSIVDSFYEAAMRSFAAGDY
                     KQSQEYLQRVLVVNPEHTEAREQLARVEILLEKQAKAMEEQQRLERIRPIRQAAMVFF
                     EQKQYEEALSKFEEILTIDTTDQEAIRYRGISRSLIAKESYNAGLKAAQDGDFPKARE
                     QLKRALHYDPDNPDIRKIYASVEDRQHEKDRLESQRVYRDALDAFLAGDLEKSLLLAQ
                     KSLELDSENLEAKRLVERLTQRRDAPKPAP"
     gene            4270..>5134
                     /locus_tag="PK362_01840"
     CDS             4270..>5134
                     /locus_tag="PK362_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73615.1"
                     /translation="MCRTQSNFRWFFLAALTAALGTSRFLWAAASPVELMQDALEAYF
                     DERYEDSVHLFAKVLAQDPKNGSAQKGLKNAQRQRAAQIQKIKEQERRNIFYVEDRLK
                     EGNLVDAYERLRDVLSRVPDHPDALDLKVKLRAKAEKAMAKARPDSSDYHYATGVLAY
                     MDGDWFKAADNWQRILDFNPDRLDLVAKVEKAKHNLAEEQRQERLRVLTEMAKTNFKE
                     GRFAEAKANWEEILTLEPHNEAAKEGIKKAVKSAEDEVQAQRMEQIQNFQVQAIDAYS
                     SGKFKESVRLWD"
BASE COUNT         1141 a         1577 c         1405 g         1011 t
ORIGIN      
        1 cgaatgggcg aaatttattg ccggttccac gtacaccttc acggcggacg gattgcccgt
       61 ggacagtaaa acctacgagt tggttttata cgctcgaaac aagaatggaa ttgaaaccgc
      121 cgggagtctc cccctgaccg tcgccctcca atccggctac cccgaggtga gcttgacgat
      181 gcccaccggc gaagttttcg tcgcgacgaa cagctacgcc gttccgatat acaccaatga
      241 aacgctcctg aaatttcgcg cccttgggtc cggccacttc aacgcgcgtg tcacggagga
      301 tgtggccatt tcaacccccg aggccctgaa cctcctgtcg ccgaatgttt acaaactgtg
      361 gaccggttgg aacgggacca ttcttccttt ccccgcgggc gtgacggcca ccaacagttt
      421 ccccgacccg ttttacgccg atccttggtc ggaatttgat tttcaaacgg aagggctttg
      481 gttcttcaac atcgccggaa ctctttccga cctgggaacg cccgccaata cctatatttc
      541 cacctcccct ttccgcgtct ttatcgacac cacccccgtg gaccccggga cgatccgcgc
      601 ggaattcggg caggacgcca ccggcacgcc cgaccacacg gaaatccttc ccgggttgcc
      661 cacgggtcac caaaaacccg aatttttctg gaccaagacg gacacccagg gcaaacccaa
      721 cccccgcagc cccatcgtgg gctggagctg gagtttttcc agcaatcccg tcgacacgcc
      781 cgttcttagc tcggcccaac ctcaattctt gcccgcgagc ttgttcgtcg ccgcttcctc
      841 cgccagttac cgggtcttta tggccaccta cattgcgaat ttcgacttgg tttttgagtc
      901 cactttttat ttcaaagtgg ccgccctgga caaagccgga aactggaccc ccatcccgtc
      961 ggaatttcaa tacaactacg tgaaggacca ggtcccgccc gatttgctcg ggtttgactt
     1021 ccccggggtg gaagtctgga cttcgtcgcc gacacggtac gaagcggtga attccaccgt
     1081 tcccgtccag gtgatctttt cggaattgat gaatttcgtt ggcggcgacc aagccatgag
     1141 catcgttcaa acccaggatc atttgggcaa tcccatgggg accaccattc cctacaccgt
     1201 ggcctgggtc gaactcccca ccggcacctc ggccgtcctg acgccgtcat tgccgggcaa
     1261 ccaatggccc acgggaagcc gtttcgtcct gcacatcgac gggttcttca cccgcgacct
     1321 cgcggggaac cgtttgaacg tgaattacga tgtgctgttc aataccgcca tggacccggc
     1381 cattgtaaac atcgggaaga gcgaggacgg caacaccacc gttaccttcc aaccgggggc
     1441 ctttgggccc aatccggccg gggcggccat cgccgacaat cccgcgacgc ggcccgtcag
     1501 cgcggcgccc ggtttggccg gaaccgttcg gaaagccgtc gaaaccatcg tgaaaacgaa
     1561 gggcggcgat taccgtaaga tcctgtccat caaacagttc gaactttacg atttgaaccg
     1621 ccaggctttg aaaacgccct tcaacggtca agtcctcttg accttccaat acaacgacgt
     1681 caacgacaac ggcatcgtgg acgagaccga ggccaaattc cccgtgaagg tgaaggactt
     1741 ggccattcac tggttggacg agaaatccgg ggcttggatc aaattgcccg aaagcaacgt
     1801 cgacaccgcg gccaaaaccg tgtccatccc gctccagcat ttctccgtct acgccctgat
     1861 cggggccccg tccctggatc tttccaacgc gcaccccttc ccggtgccgt acaaagcgtc
     1921 caccgatccg gccggcatca cgttcacggg cctttccagc attgcgacgg tcaaaatctt
     1981 taccttggac ggccggctgg tgaaaaccct gaccgacgac tccggcgccg gtttcgtccg
     2041 ctgggacccg gtgaacaacg aaagcggcga ccccgtggcc agcgacgtct atctctatgt
     2101 cattgaaaac gatcagcagc ggcggatcgg aaagttgatg gtgatccgat gaaccgtcga
     2161 catttatgga ccggcctcgg cgccttcctc ctgggggtcg ggtcgctttg ggccaaggcc
     2221 tcgggctcca gcacggccaa ttttttgaaa ctcggcgtgg gcggccgcgg cgtggccatg
     2281 ggcgaggccc acacggcggc ggtggacgac gtcatgtcgc tttattggaa cccggcggga
     2341 ttggggtacc tttacctgaa cgaagtcggg ttcatgcaca acaattttgt tcaagggatc
     2401 tcccaggacg tgttctatta cgcccgcccc acggaaacca aagggacctt cggcgccggg
     2461 gtatcgatgc tccgcatcaa cgacatcgcg gggttcgacg cctccgacat tcccacgggc
     2521 aacgtgcagg cctcggacac gatgctgact tttgggtggg ggaaatccat ggagcagttg
     2581 gtgtggttcc gaggcatgcg cgtgggcgcc aaccttaaaa tccttcgcaa aaccctggac
     2641 cgcacctcgg cgttgggcta catgaccgat ttgggccttt tgtatgaaac ccggggcggc
     2701 tggttccacg ggctgaacac cgggtttgtc attcaaaatc ttgggacggg cctctccatg
     2761 ggcggccaaa aatccaattt tccgcttcaa atgaagctgg ggttcgccta tccgatgttc
     2821 ggtgacaacc tggtgctcgc gacggacctg gtgctgccga cggacggcag tctctacatc
     2881 aacatgggaa ccgattaccg catatgggat attttggcct ttcgcctcgg ctaccgcggg
     2941 tccaacgatt tggattcggg cctcacctac ggcgtccgtc tgggcaacga acgccttcac
     3001 atggattacg cctttgtgcc cttcggccct ttcggggaca gccaccgggt cagcctgggg
     3061 gtgcgtttcg gcaaggcctt ccgcgaaggg aaggttgccg atcaaattca aacggcgtat
     3121 atgcgggccg aggcgaaata cggtcagggt ctcctggtcg acgcctacat gcaagccacc
     3181 caaattctgg acgtagcgcc ctggcatcgg gcgtccaaat tgcttacgaa gaggattgag
     3241 agcgaattaa aggccatgga aaccggggcc cgccgccagc agctacaggc ccaggtggat
     3301 gaacactacg cccgcggcga gcaactcttc caaatcgacg atttgctcca cgccaaacgc
     3361 gaattcgaag ccatattggc tttgcaggcc gatcacatgg gcgccaaaac ctatttgaat
     3421 cgcattgaag aacggttcaa gagcatcgtg gacagcttct acgaagctgc catgcgttct
     3481 ttcgccgccg gggactacaa acagtcccag gaatacctgc agcgcgtcct ggtggtcaac
     3541 ccggaacaca ccgaagcccg ggaacaattg gcccgggtgg aaatcctgct cgagaagcag
     3601 gccaaagcca tggaggaaca acaacggttg gaacgtattc gacccattcg ccaggccgcc
     3661 atggttttct ttgaacaaaa gcagtatgaa gaggccctct ccaaattcga ggaaatcttg
     3721 accatcgaca cgacggacca ggaagccatc cgttaccgcg gcatttcccg cagcttgatc
     3781 gccaaggaat cttacaacgc ggggttgaag gccgcccaag acggcgattt ccccaaggcc
     3841 cgggaacagc ttaaacgggc cctccattac gacccggaca atccggacat ccggaaaatt
     3901 tacgcctcgg tggaagaccg ccagcatgaa aaagaccggt tggaatccca gcgggtttac
     3961 cgcgacgccc tggacgcctt cctggccgga gatctggaaa aatccctgct tctggcccag
     4021 aaatcccttg agttggattc cgaaaacctg gaagccaaac gcctggtgga acgcttgacc
     4081 caacgccggg acgcccccaa acccgctcct taaagggcgg gttgacaaaa gatgttttat
     4141 gagggaaact taattgaaat tgatcatgcg ttgagcaacg atcggcggac ccgtggccaa
     4201 aaaaccggcg gcgggtctcc ttattgttcg atcccccgag tcgtttcggg aaggcggaaa
     4261 ccatcaccca tgtgtcgcac ccagtctaat ttccgttggt tttttctcgc cgccctgacg
     4321 gcggctctcg gaacctcccg ctttctttgg gcggccgcct cccccgtgga actcatgcag
     4381 gacgccctcg aggcttattt cgacgagcgg tatgaagact ccgtccatct gttcgcgaaa
     4441 gtgctcgccc aggaccccaa gaacggatcg gcccaaaaag gcctcaaaaa cgcccagcgg
     4501 cagcgcgcgg cccaaatcca aaaaatcaag gagcaggaac gccgcaatat tttctacgtg
     4561 gaagatcgcc tgaaagaagg gaacctcgtg gacgcctacg agcgcttgcg cgacgttttg
     4621 tcccgggttc ccgaccaccc ggacgcgctg gatctcaaag tgaaacttcg cgccaaggcc
     4681 gaaaaggcca tggccaaagc gcggccggac agctccgatt accattacgc cacgggcgtg
     4741 ctggcctaca tggacggcga ctggttcaaa gccgccgaca attggcaacg cattttggat
     4801 ttcaaccccg atcggttgga cttggtggcc aaggtggaga aagccaaaca caatttggcc
     4861 gaggaacagc gccaggagcg ccttcgcgtg ctgaccgaaa tggccaagac gaatttcaag
     4921 gaagggcgct tcgccgaggc caaagcgaat tgggaagaaa ttttaacgtt ggaaccccac
     4981 aacgaagccg ccaaggaagg gattaaaaag gccgtcaagt cggcggaaga cgaagtccaa
     5041 gcccaacgca tggagcaaat tcagaatttc caggttcagg ccatcgacgc ttattcgtcc
     5101 ggaaagttca aggaatccgt tcgtctctgg gacc
//
LOCUS       DAOQLS010000061         5100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7527_length_5100_cov_5.462438, whole genome shotgun sequence.
ACCESSION   DAOQLS010000061 DAOQLS010000000
VERSION     DAOQLS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7527_length_5100_cov_5.462438"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..789
                     /locus_tag="PK362_01845"
     CDS             142..789
                     /locus_tag="PK362_01845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNC73616.1"
                     /translation="MRWKPLSEVVGFYGLVLLSIWVGQSLGLRGQVWIGAVLLLGATV
                     VSSRKHGDGRDRLGLGRNTLGPAARLTLIYAGPALLLLAIVALFRPAPPWPKILFGVL
                     GYPLWAFAQEYALLSFAANRLEEGLGQKPALVSTVNGLLFALVHTPNPVLMGACLLSG
                     WVFTKIFLKTRNIFPLALAHALGGFLLSWILLNQYNGMMVGPAYWKWAGTPGPHP"
     gene            complement(1015..1206)
                     /locus_tag="PK362_01850"
     CDS             complement(1015..1206)
                     /locus_tag="PK362_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73617.1"
                     /translation="MLVVAIIGLLAAIAIPKFANLVVKAKEASVRGKSVRFVAPCPFT
                     TRTAMEIDFIRLVPISSTL"
     gene            complement(1266..1760)
                     /locus_tag="PK362_01855"
     CDS             complement(1266..1760)
                     /locus_tag="PK362_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73618.1"
                     /translation="MRREPFLRSVFGAFTLIELMLTVAIIGLLAAIALPKFANLVAKS
                     REAAAKGSLGALRSAINILYIDRDQAYVSSTTAGWSNIPLWLVPRYIDAIPTAGLPHH
                     HHGTGSDVLVLPGDPIDAYWDFTSPTPTPWFWQVDPPQTIWTGNVYINCTHTDTRGVT
                     ISLW"
     gene            complement(1910..2344)
                     /locus_tag="PK362_01860"
     CDS             complement(1910..2344)
                     /locus_tag="PK362_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC73619.1"
                     /translation="MRTRWAPAFTLVEMMIVVAIISLLAAVAFPRFSNMMGKSREAAL
                     RGKLGTVRSAISIYYADTEGVFPFDVGVALTSGGRYLNEMPLVQIPNYTGHAANRLVG
                     TACSEWDCTDNSGAAYRYDPNNGHLAINCEHTDSTSRTWSTW"
     gene            complement(2341..2799)
                     /locus_tag="PK362_01865"
     CDS             complement(2341..2799)
                     /locus_tag="PK362_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNC73620.1"
                     /translation="MALRWTKLLYGTGVELIDAQHRRLFEWMNKLIDAFDRADASDIS
                     SILGDLNAYAVKHFRCEERTMAKGNCAALCLNQTEHREFSNKLALLKKEFEINGPTPA
                     VAREFKFFILGWVRTHIQAVDVHLQCTIKQGFRLEAPPSTNVAADGAPPS"
     gene            complement(3006..3467)
                     /locus_tag="PK362_01870"
     CDS             complement(3006..3467)
                     /locus_tag="PK362_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73621.1"
                     /translation="MSENRSLSGFTLIELMIVVAIIGILAAIALPKFSNLVIKSKEAS
                     VKGKLGALRGALNIYYADNEGTYPRVDLAALRTGGKYLDAIPSISIPTVPIHTSANIV
                     SGIADVADDSSFQGVGVAWKTNGSSPGSIPASAVWVNCTHTDSSGRTWSLW"
     gene            complement(3614..4957)
                     /locus_tag="PK362_01875"
     CDS             complement(3614..4957)
                     /locus_tag="PK362_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC73622.1"
                     /translation="MKSENPPAVDQRRSPFRFSLLWRTLRYRNYRLFFAGQLVSLIGT
                     WMTNTATVWLVYRLSGSALMLGLVGFSSQIPAAVLSPLAGIYIDQWNKHRVLVATQVL
                     SMLQSFALAALALSGKITVGWLIGLNALQGVINAFEMPCRQSFVISLVEEKENLGNAI
                     ALNSSMFNAARLLGPSIAGIVIAWIGEGWCFFADGVSFLAVLASLLAMRLAPRPAGQR
                     STTALAEFREGWRYTFGSPPLRAIITLLALTSLVGVPYTVLVPIFAGKLLGGGPHTLG
                     FLMAAAGAGALLAALWLAARRSVLGLTRVVPWATATFGGGLILFSLSRWLPLSLLLMM
                     VAGFGFMTQMAASNTVLQTIVEDDKRGRVMSLFVFAFLGTAPLGSLLAGGLADRLGAP
                     WTLRLGGLGCVLGSLWFFRQLPQLRLAIRPIYLKLGILREVAEGIERVSVLEKLE"
BASE COUNT         1196 a         1411 c         1477 g         1016 t
ORIGIN      
        1 gccggcgtgc cgggaatcca tttttacacc ctcaatcgct cccgatccac cgccgaggtt
       61 ctaaaaaccc ttcgggctcg ctgaaatccg aacaccgctc cagggcttga ccccgacttc
      121 cggggggcct acccttacat tatgcgatgg aaacccctgt ccgaagtagt cgggttctac
      181 ggactggttc tgctttctat ttgggtcggg caatccttgg gtctgcgggg tcaagtttgg
      241 atcggtgccg ttctcctgct gggggccacc gttgtttcaa gtcgaaaaca tggcgacggt
      301 cgggaccgat tgggactggg gcgaaacacc ttggggccgg cggcccgttt gaccttgatt
      361 tacgcgggac cggctttact gcttttggcc atcgtggccc ttttccgccc ggccccgccg
      421 tggcccaaaa ttctttttgg cgtgttggga tatcccctct gggccttcgc ccaggaatac
      481 gccctgttga gtttcgcggc caatcggttg gaggaaggtt tgggacaaaa accggcgttg
      541 gtctcgacgg tgaatggcct gcttttcgcc ttggtgcaca cgcccaaccc ggtgctcatg
      601 ggggcgtgcc tattgagcgg ttgggttttc acgaaaatat ttcttaaaac ccgaaatatc
      661 tttcctctgg ccctggccca cgccctgggg ggatttttgt tgagttggat tttattgaat
      721 cagtacaacg gcatgatggt cggtcccgcc tattggaaat gggccggaac gcccggtccc
      781 catccataga cttttaaaac gtactccaaa tcactccctt ggtatcgctg tgggtgcagt
      841 tcagatgaaa ctcggagtaa tccgggacga ccataatacc gtagggtcgc ggcgccaagg
      901 gggaccaccg tgaatcgtcc ggcataaccg aaatggtgaa cccccgcccg gccgggtgaa
      961 gatcgggatg ggccggatgc tcgatcgttg gaatatcatc gatgcatttg gggatcaaag
     1021 ggtcgaagaa atagggacta aacggataaa atcgatttcc atcgctgtcc gcgtagtaaa
     1081 tggacatggc gctacgaacc gaaccgattt tccccggaca gaggcctcct tggctttgac
     1141 caccaagttg gcgaattttg gaatggcgat ggccgccaaa agtccgatga tcgccaccac
     1201 cagcattaat tcaataagag taaaaccgcg caaatatcga aatcggcgtg gggtccttcc
     1261 taaaatcacc ataaactgat cgtcactccc cgcgtgtccg tgtgggtgca attgatgtag
     1321 acgtttcctg tccaaatcgt ctggggcggg tccacctgcc agaaccacgg cgtcggcgtg
     1381 ggggacgtga aatcccaata agcatcgatg gggtcgcccg gcagaaccaa aacgtcgctc
     1441 ccggtcccgt gatggtggtg ggggagcccc gccgtcggaa tcgcatcgat gtatcgagga
     1501 acaagccaga gggggatatt ggaccatcct gccgtcgtgc tggaaacgta ggcctggtcc
     1561 cggtcgatat agagaatgtt gatggccgaa cgaagagcgc ccagggatcc tttggcggcg
     1621 gcttcccggc tcttggccac gagattggcg aattttggca gggcgatggc ggccaacaac
     1681 ccgatgatcg ccaccgtcaa catcaattcg atcaaagtaa aggccccgaa aactgaacgg
     1741 aggaatggtt ccctccggat tcctgctccg gagaaaaacc ggcgccggcc gttgcgcaag
     1801 acctttttct tcaaaaatcc agcgaacatg gccccccctg ttcatcaggg taggccccgg
     1861 gggccattct gtcaagctga ttagcttttg tattgaaaaa taaatatgtt taccaggtgc
     1921 tccaggtgcg cgaggtcgag tccgtgtgct cgcaattgat ggccaaatgc ccattattcg
     1981 ggtcgtaccg ataagccgcc cccgagttgt cggtgcaatc ccattccgaa caggccgttc
     2041 ccaccaaccg attggccgcg tgccccgtgt agttcggaat ctggaccaac ggcatttcgt
     2101 tcaaataccg accgccgctc gttagggcga cgccaacatc gaaagggaaa accccctccg
     2161 tgtcggcgta gtaaatgctg atggcgctgc ggacggtgcc cagcttcccc cgcagggcgg
     2221 cctcccggga tttccccatc atattggaaa aacgcggaaa ggccaccgcc gccaaaagac
     2281 tgatgatcgc gacgacgatc atcatttcga ccaaagtaaa cgccggagcc caacgggtcc
     2341 tcatgaaggc ggcgccccat cggccgcgac gtttgtcgac ggaggcgcct ccagccgaaa
     2401 cccctgtttt atcgtgcatt gcaaatgaac gtcgaccgcc tgaatgtggg tgcgaaccca
     2461 cccgaggata aaaaacttaa attcccgggc cacggccggg gtcgggccgt tgatttcaaa
     2521 ttcttttttc aatagggcca gtttgtttga aaattcccgg tgctcggttt ggttaaggca
     2581 aagggccgca caatttccct tcgccatggt tcgttcctcg cagcgaaaat gtttgacggc
     2641 gtaggcgttc aggtccccga gaatggagga aatatcggac gcgtcggccc gatcgaaggc
     2701 atcgattaat ttgttcatcc attcgaatag ccgccggtgt tgggcatcga tcagctcgac
     2761 gccggtgcca taaagtaatt tcgtccaacg tagggccaag aggttcctcg ttcaatgaca
     2821 atatgattaa gcatcgaaat tatcggtatt tttttgattt atttaaggag gtttgggtgg
     2881 gatggataaa aacagcgtaa gcaacgatca aatctaaata tcaatacgtt ttttcttacg
     2941 gtcccaaggg aatgccgaaa acggagaagg aggtgttcgg ccggtggcgg ccgttcaatg
     3001 aaaaattacc aaagactcca tgtccggcca ctggaatcgg tgtgggtgca attgacccaa
     3061 acggcggaag ccggaatgga cccgggggac gacccgtttg ttttccaggc cacaccgacc
     3121 ccctgaaacg aggaatcgtc ggccacgtcc gcgattccgc tcacgatgtt ggcggaggtg
     3181 tgaatgggaa cggtgggaat tgaaatggag gggatcgcgt ctaagtattt tcctccggtg
     3241 cggagggccg ccaggtcgac ccggggataa gtcccctcgt tgtcggcgta ataaatattc
     3301 aaggcgccgc gcagggctcc caattttcct ttcacggaag cttctttgga tttgatgacc
     3361 aaattggaaa attttggaag agcgatggcc gccaggatgc caatgatcgc cacgacgatc
     3421 atcagttcga tcagcgtaaa gccggagagg gagcggtttt cactcatgaa atgttcaccc
     3481 aacaaggcta acccctacta ggacatattt ccaggagaag gcaaggcggg catactattt
     3541 tgaatctaaa aatagataca aaaacataac actgttcaca gaggagaaag ccgccgaagc
     3601 gaaatgggta aagctattcc agcttctcca acacggaaac ccgctcaatg ccctccgcca
     3661 cttcccgcag aatgcccaat ttcaaataaa tggggcgaat ggccaagcga agttggggca
     3721 actgccgaaa aaaccaaagg gacccaagaa cgcaacccaa tccgcccaag cgaagcgtcc
     3781 aaggggctcc caggcgatcg gccagacccc ccgccagcag gcttcccagg ggagccgttc
     3841 ccaaaaaagc gaaaacgaac aaactcatga cccgccctct tttgtcgtcc tccacgatgg
     3901 tttgcaacac cgtgttgctg gcggccatct gcgtcataaa accgaatccg gcgaccatca
     3961 tgaggaggag cgacaggggc agccatcggg aaagggaaaa aagaatcaat ccaccgccga
     4021 aagtcgccgt ggcccagggg accacgcgcg tcaggcccaa aaccgaccgg cgggccgcca
     4081 accagagcgc cgccaggagc gccccggccc cggcggccgc catcaaaaat cccagcgtgt
     4141 gcggcccgcc ccccaggagt tttccggcga agatgggaac cagcacggtg taggggaccc
     4201 ccacgaggct ggtcagagcc aaaagggtga tgatcgctcg aagagggggg gacccgaagg
     4261 tgtagcgcca accttcccgg aattcggcca acgccgtggt cgatcgctgg cccgccgggc
     4321 gcggggccaa tcgcatggcc aggagggaag cgagaacggc caaaaaactc actccgtcgg
     4381 cgaaaaagca ccacccctcc ccgatccaag cgatcacgat tccggcgatg gacggcccca
     4441 gaaggcgcgc cgcgttgaac atggaggaat taagggcgat ggcgttgccc aggttttcct
     4501 tttcctccac cagggaaatg acaaaggatt gtcggcaagg catttcaaaa gcgttgatga
     4561 cgccctgaag ggcgttcagg ccaatgagcc atccgacggt aatttttccg ctaagcgcca
     4621 gggccgccag ggcgaaggat tggagcatgg acaacacctg cgtcgccacc aggacccggt
     4681 gcttgttcca ctggtcgatg taaatgcccg cgaggggcga caaaaccgcc gcggggatct
     4741 ggctggagaa gccgacgagt cccaacatca gggccgaacc ggacaggcga tagacgagcc
     4801 acaccgtcgc cgtgttggtc atccaggtgc cgatgaggga aaccaactgg ccggcgaaaa
     4861 acaggcggta gtttcggtaa cgcaaagtcc gccacaggag cgaaaaacga aaggggctcc
     4921 ggcgctgatc cacggccggc ggattttccg atttcataag aagattttat ctgttgtgga
     4981 cgcgggcgtg gcgcggcgaa agggggagag ggcgaaaccg tgttctacca caagctccat
     5041 gtttgaccgt tggtgtcggt gtgcgtgcaa tttacgtata gctggccggt caaggggaaa
//
LOCUS       DAOQLS010000062         5095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7540_length_5095_cov_4.930556, whole genome shotgun sequence.
ACCESSION   DAOQLS010000062 DAOQLS010000000
VERSION     DAOQLS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7540_length_5095_cov_4.930556"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PK362_01880"
     CDS             complement(<1..591)
                     /locus_tag="PK362_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HNC73623.1"
                     /translation="MIIEPQSKGFICLTAHPAGCAKNVALQIEHVSRGGRRSGPKNVL
                     VIGASTGYGLASRIAAAFGYGANTLGVFFEKPAEGKRTATPGWYNTAAFESAARAAGL
                     RAVSLNGDAFSDELKAQTVAALKKDFGPVDLVVYSLASPRRTHPREGVVFTSALKPIG
                     RAYTSKTVNFQNGQMSEVTLDPATVAEKLTKIDAACD"
     gene            complement(597..2455)
                     /gene="typA"
                     /locus_tag="PK362_01885"
                     /pseudo
     CDS             complement(597..2455)
                     /gene="typA"
                     /locus_tag="PK362_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
     gene            2626..3510
                     /locus_tag="PK362_01890"
     CDS             2626..3510
                     /locus_tag="PK362_01890"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNC73624.1"
                     /translation="MANVFHLMNPSLRNLPRVKLVTKVTRGMAPWALVARQHPHHTET
                     LLDWAVKTGVTRLVVWGGDGTFHRVVRGLHERGVLDKIELGLVPMGTCNDLARRLGLS
                     WECWGRWETQSPQGRLAALSLGRMSWRAQADAPQPDGSDVFINNAGFGRPRTSYKRKD
                     SPWRVLRSFKSLGVTVRWGDGHLTARAYFGLAALGPYFSGGLHFEKDLSPEDGVLRFY
                     LVPARSKIRLALRLARGRLGAPLFDEKVTKISAPRLSVESESPLWPQADGEPPAAKAA
                     RRVDFELLTPRAKLWVTH"
     gene            3520..4011
                     /locus_tag="PK362_01895"
     CDS             3520..4011
                     /locus_tag="PK362_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNC73625.1"
                     /translation="MIRAYLDVETDERQALTVVGVFRPDRGHRQWVRPQFSRLELLNY
                     LTDVELLMTYNGARFDLPLVRDQLGADLATLFRHRDLMLDCWAQNLYGGLKKVERRLG
                     IHRDTEGVDGWAAIRLWHAHRAGDAEALDLLLRYNREDVENLEALARKLGVLPAAAAG
                     GRS"
     gene            4008..4880
                     /gene="folD"
                     /locus_tag="PK362_01900"
     CDS             4008..4880
                     /gene="folD"
                     /locus_tag="PK362_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNC73626.1"
                     /translation="MTATLLDGKAAAARVLERVTEKAQALKARGRPAGMATVLVGDDP
                     ASHIYVGQKIKKCEAAGMASIHVPLPATVSETELLAQVDKLNRDPRVHGIIVQLPLPK
                     GLNAERVLLALDPAKDADGLHPSNQGRWAQLKSWSDVLASGIPLPCTPAGVMELLRQN
                     NVSVAGKRAVVVGRSTLVGKPIALMLLAADATVTVAHSRTANLADVCRSADILIAAIG
                     QDRMIQGDWVKPGAVVVDVGMNRTAAGLFGDVDFAAARDRAGALTPVPGGVGPMTVAL
                     LLWNTVLAAERAAR"
     gene            4933..>5095
                     /locus_tag="PK362_01905"
     CDS             4933..>5095
                     /locus_tag="PK362_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC73627.1"
                     /translation="MKGKVKWYNRVKGYGFLIPEDGSAEVFVHHSAIQAKGKDKVLVD
                     GQAVEFEVTQ"
BASE COUNT          847 a         1546 c         1694 g         1008 t
ORIGIN      
        1 gtcgcaggcg gcgtcgatct tcgtgagctt ctcggccacg gtggcggggt cgagggtgac
       61 ctcggacatt tggccgtttt gaaagttgac ggttttggag gtgtaagccc gcccgatggg
      121 tttgagggcc gaggtgaaca cgacgccttc ccggggatgg gtgcgccggg gcgaggccaa
      181 gctgtaaacc acgaggtcca cggggccgaa atctttttta agggcggcga cggtctgggc
      241 cttcagttca tcggaaaaag cgtccccgtt caggctgacg gcccggagcc cggcggcccg
      301 ggcggcggat tcgaaagcgg cggtgttgta ccaccccggc gtggcggtgc gcttcccttc
      361 ggcgggcttt tcgaaaaaca ccccgagggt gttggccccg tagccgaagg cggcggcgat
      421 ccggctggcc aacccgtacc ccgtcgaggc gccgatgacc aaaacgtttt ttggaccgga
      481 gcgacggccc ccccgggaca cgtgctcgat ctgcagggcg acgttcttcg cgcaccccgc
      541 ggggtgcgcc gtgaggcaga tgaatccctt ggattgcggc tcgatgatca tgcgggtcag
      601 ccttcctctt cttccttctc ggattttcgc cgacgactgt gatccaattc ctttttccgc
      661 aggcggattg ttttcggtgt gatttcggcc aattcgtcct cctcgatata ggccatggcc
      721 tgttcgaggc taaaatccga ggcggggtca gctgaaccaa atcgtcggat cccgcggccc
      781 gcatgttcgt gagggctttc tttttgcacg ggttgacgat catgtcgttt tcccgggcgt
      841 tttggccgac gatcatgccg gcgtagatgt cggttccggg cgccacgaac aacacggagc
      901 gggcttgaag gctttcgagg gcgtaggacg tgcccaggcc ggattccttg gcgatcaaca
      961 cgccggccaa acgccgggtc acgacgttgg acctcggaat gtagccgtgg aagctgtggt
     1021 gcatcaatcc cgtgcccttg gtttgggcca ggaattcgga tttaaacccg atgagggccc
     1081 gggcggcaat gacgccttcg attcgcaggc gcgttccccc gccctcggcg tgcatgttct
     1141 tgatctccag gccgcgcttg cccagatttt ccatcacggc gccctggtag gattgttcaa
     1201 tgtccaccac caggtattcc gccggttcca gggttttccc gttttccgtt ttcaagatcc
     1261 cctgggggcg ggaaacggcc agctcaaagc cctcccgccg catggtttcg atcaggaccg
     1321 acaggtgcaa ttcgccccgg ccggagactt tgaaggtgtt ttgcgtgagc tcctctacgc
     1381 ggaggcccac gttggttttg acttcctgtt ccagccgctc gcgcaggtgc cgcgaggtca
     1441 ggaactttcc gtcccgcccg gaaaaggggc tgtcgttcac catgaactcc accgaaaggg
     1501 tgggttcgtc gatcagcatg ggggggaggg ccacggggtt ttccgcgtcg gccaccgtgt
     1561 cgcccacccg ggcctcttcc atgcccgcga tggccacgat gtcgccggcg aaggcctttt
     1621 caatctcccg ccgctccaag ccgaagaagc cgtaaatccg cgtggcccgc atggggaggg
     1681 cccgcccgtc ggtcttcatc agcatgacgg attggttgcg ggacaccgtc ccggccaaaa
     1741 tgcggccgat ggcgatgcgc ccgacgtagg agctgtagtc caccatggtg gtcagcatct
     1801 ggagggggct ctgggggtcc gccacggggc cgggcacgtg ccgcagaatg gtttcaaaga
     1861 ggggtttcag atccgtcgtc gggtgtttta aatccagggt ggcccagccg tttttccccg
     1921 aggcgtagac gatgggaaaa tccagctgct gttcggaggc gccgaggtcg acgaaaaggt
     1981 caaaggtgtt gttgagggct ttggccgggt cggcgaaagg gcggtcgact ttgttgatga
     2041 ccaccacggg ccgcaggccc agctcaaggg atttgcggag cacgaaccgc gtttggggca
     2101 tggggccgtc cagggcgtcg atcaggagga gcaccccgtc caccatggtc aaaatgcgtt
     2161 cgacttcgga tccgaaatcc gcgtgaccgg gcgtgtcgac gatgttgatg cgcgtgtcgc
     2221 cgtactgcac ggaggtgcat ttggccagaa tggtgatgcc ccgctcccgt tcctggggat
     2281 tggaatccat caggcattcc tgggcctggt cggctttgac cttgaactcg cccgtctggc
     2341 ggaaaagggc gtccaccagg gtggttttcc cgtgatcgac gtgggcgatg atggcgatgt
     2401 ttcgcacgtc cgggcgcctg ggcgctccgg tgactttcgg tgtaacggtt tccatttttt
     2461 ccataagtgt ggtttccata taggcaaaat ccgaccccgg ggtccgatgc gtggaccgct
     2521 tcaaccctgt gcttcgatga ggggaaaagg gggagggtaa actccgacca acagggtcat
     2581 attataccac aagtccttgt tttttcgttt aattatcttc cacccatggc caacgtcttt
     2641 catctgatga acccgtccct gcgcaatctg ccccgggtga agctcgtgac caaagtcacg
     2701 cgcggcatgg cgccctgggc cctcgtggcg cgccagcacc cccaccacac cgaaacgctg
     2761 ctggattggg cggtcaagac gggggtgacc cggttggtcg tttggggcgg cgacggcacc
     2821 tttcaccggg tcgtccgggg ccttcacgag cggggcgtcc tggacaaaat cgaactgggg
     2881 ctcgtcccca tgggaacgtg caacgatctc gcgcgccgcc tgggcctttc ctgggaatgt
     2941 tgggggcgct gggaaaccca atccccccag gggcgactgg ccgcgctgag cctcggccgc
     3001 atgtcctggc gggcccaggc cgacgcgccc cagcccgacg ggtccgacgt gttcatcaac
     3061 aacgccggct tcgggcggcc gcgaacctcc tacaaacgga aagattcgcc ctggcgcgtt
     3121 cttcgttcct tcaaatctct cggggtcacg gtccgatggg gggacggtca tttgaccgcc
     3181 cgggcctatt tcgggttggc ggccctgggc ccctattttt cgggggggct ccatttcgag
     3241 aaggacctct ccccggagga cggcgtcctt cgtttttatt tggtcccggc gcgttccaaa
     3301 atccgcctgg ccctgcggct ggcccgggga cgcctcgggg ccccgctgtt cgacgaaaaa
     3361 gtcacgaaga tttcggcccc ccgcctgtcg gtcgaatcgg aatcgcccct gtggccccag
     3421 gccgacgggg aaccccccgc cgccaaagcg gcccggcggg tggatttcga gttgctgacc
     3481 ccccgggcaa agttgtgggt gacccattga ggcgggacgg tgatccgcgc gtacctggac
     3541 gttgaaaccg acgagcggca ggccctgacg gtggtgggcg ttttccggcc cgaccggggg
     3601 catcgccagt gggtccggcc ccaattttcc cggctggaat tgctaaatta tctgaccgac
     3661 gtggaactcc tgatgacgta caacggggcc cggttcgatc tgccgctggt tcgcgatcaa
     3721 ttgggagcgg atttggcgac cctgtttcgg caccgggatt taatgctgga ctgttgggcc
     3781 caaaatctgt acggtggact caagaaagtg gagcggcggc tgggcatcca ccgggacacc
     3841 gagggggtgg acggctgggc ggccattcgt ttgtggcacg cccaccgggc cggcgacgcc
     3901 gaggcgttgg atttgttgtt gcgttacaac cgggaagacg ttgaaaattt ggaagcgttg
     3961 gcgcgcaagc tgggggttct ccccgccgcc gccgcgggag gacgatcgtg accgccacac
     4021 tcttggatgg aaaagccgcc gccgcgcgcg tgttggagcg cgtgacggaa aaagcccagg
     4081 ccctcaaggc ccggggccgc cccgcgggga tggccaccgt gctcgtgggg gacgacccgg
     4141 cgtcccatat ttacgtcgga caaaagatta aaaaatgcga agccgccggc atggcttcca
     4201 ttcacgtgcc cctgccggcc acggtgagcg aaacggaact gttggcccag gtggacaagc
     4261 tcaaccgcga cccgcgcgta cacggcatca tcgttcagtt gccgcttccc aaaggcctga
     4321 acgccgaacg ggtgttgctg gccctggacc cggccaagga cgccgacggc ctgcacccct
     4381 ccaaccaggg gcgatgggcc cagttgaaat cctggagcga cgtcctggcc tcgggcatcc
     4441 cccttccctg cacgcccgcc ggggtcatgg aattgctccg gcaaaacaac gtgtccgtcg
     4501 cggggaaacg cgcggtggtc gtgggccgct ccaccctggt gggaaaaccc atcgccctga
     4561 tgctgttggc cgccgacgcc acggtcaccg tggcccattc ccgcaccgcg aatttggccg
     4621 acgtgtgccg ctcggcggat attttgatcg ccgccatcgg gcaggatcgg atgatccagg
     4681 gcgactgggt gaagcccggc gcggtggtgg tggacgtggg catgaaccgc acggccgcgg
     4741 ggcttttcgg cgacgtggat ttcgcggccg cccgggaccg cgccggcgcg ttgacgcccg
     4801 tccccggcgg tgtgggcccc atgacggtgg ccctcctgct ttggaacacg gttttggccg
     4861 ccgagcgcgc ggcccgctga tttcgggaac ggatttttct tcggagagtt ccctggagag
     4921 aggagaggtt ccatgaaagg taaagtcaag tggtacaacc gcgtcaaagg ctacgggttt
     4981 ctgattcccg aggacggcag cgcggaggtg tttgtgcatc attcggccat ccaggccaaa
     5041 ggcaaagaca aagtcctcgt ggacggtcaa gccgttgaat tcgaagtcac ccagg
//
LOCUS       DAOQLS010000063         5069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7612_length_5069_cov_3.886518, whole genome shotgun sequence.
ACCESSION   DAOQLS010000063 DAOQLS010000000
VERSION     DAOQLS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7612_length_5069_cov_3.886518"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PK362_01910"
     CDS             complement(<1..829)
                     /locus_tag="PK362_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73628.1"
                     /translation="MMAYAVRLLDAFAQGHLVGFSRLLRAAALGLLVLAAVGPLGPAA
                     VRWAIRRWPWWRCHKCGRFNRKAHKVCDKCLAGLSDAVLRPPPVPRPGAFYRWVLRPW
                     RWVWAILFYPLALLAVSLTGVWGGDHRALELSAAALFLVFGVGAHAAVAAALGPWSAF
                     VELRLVRVFWAFILAGFSAGALFLWCMSPLAALPGNRTFAVSPAGGLRFVGAARDEIR
                     LLRDPRDGAVIVPVAGAVFRWPAFGIERWVVTRIAGTPTTPGPVRWLTRRLATPARGG
                     "
     gene            complement(826..1437)
                     /gene="rdgB"
                     /locus_tag="PK362_01915"
     CDS             complement(826..1437)
                     /gene="rdgB"
                     /locus_tag="PK362_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNC73629.1"
                     /translation="MPRPAVVIATHNRHKLTEIRKILKSPTFRVRGLDDFPPPYTVRE
                     TGKTLAANALLKARKAARVTGLLSLSDDTGLDVAALGGRPGVYSARFAGPNCSFEDNN
                     RKLLRLLRGVPRSKRRAVFRTVVAAVFPDGREKIFEGRCPGWITDDFQGVHGFGYDPV
                     FAPGRSRRTFAQLTLAQKNRVSHRARAFQKARAFLLKTFRRAR"
     gene            complement(1460..2419)
                     /locus_tag="PK362_01920"
     CDS             complement(1460..2419)
                     /locus_tag="PK362_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC73630.1"
                     /translation="MRIGRAAAPVPGEDSPDIMKITFWGTRGSIATPGPDTVRYGGNT
                     ACVELSDDKNLLVFDVGTGLRLLGEELCRRIPAGKINAHLFISHFHFDHIIGFPFFRP
                     LYTPGNTFTLYGCEGTGRKLETIFVGQLSPEYFPVSLSEIPAKLNFVQLTTRPLPLNG
                     WTVTPAYVNHPGLALGYRVDNGKSRVVYMTDNEPFRYLLRQQAKKTPIYDDLDRGEVE
                     LEREDLMLVDFLEGADVLIHDAQYTIEEYRTKTGWGHSFYDFAVEMALRGKVKKLILF
                     HHDPLRTDKDLDLQVQRARSIVAQRGAALEIEAAFEGLEIDLP"
     gene            complement(2547..3611)
                     /gene="gap"
                     /locus_tag="PK362_01925"
     CDS             complement(2547..3611)
                     /gene="gap"
                     /locus_tag="PK362_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNC73631.1"
                     /translation="MAVKIAINGFGRIGRLVFRALVEQGLLGKQLDVVAVGDIVPADN
                     LAYLLKYDSTQGRFHGTVGSKKSSPDKVEDDVLIVNGHDIRVVSAKDPSGLPWKDLGV
                     ELVIESTGLFTDAEKAKGHLAAGAKKVIISAPAKNEDLTVVMGVNENKYDPAKHHIIS
                     NASCTTNCLAPLVHVLLKEGFGIEEGLMTTVHSYTATQKTVDGPSKKDWKGGRTAAQN
                     IIPSTTGAAKAVALVCPEVKGKLTGMAFRVPTPTVSVVDLTVKTTKDTSYAEISAAMK
                     KASETYLKGVLEVTSDEVVSSDFIHSSSSSIFDQGSGIELNKRFFKLVSWYDNEWGYS
                     NRVVDLVKYIVNKMVAVAAR"
     gene            complement(3765..4136)
                     /locus_tag="PK362_01930"
     CDS             complement(3765..4136)
                     /locus_tag="PK362_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC73632.1"
                     /translation="MARILVGEDDDNLREYFRFVLENSGHQVSAVGDGQAGLNACAAE
                     PPDLVILDVMIPEVNGYEVCHRIKSDAKTQKAKVLMVTAKTFAADRLEAKNAGADGFL
                     AKPVAPLDLLETVKTLLAPAP"
     gene            4244..>5069
                     /locus_tag="PK362_01935"
     CDS             4244..>5069
                     /locus_tag="PK362_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC73633.1"
                     /translation="MELPTVYAPDATESKWYDFWESRGYFAARPDAAKKPFTVVIPPP
                     NVTGSLHMGHALNNTLQDVLVRRRRMKGDNALWIPGTDHGGIATQNVVEKMLKKEGKS
                     RHDLGRAAFLERMWAWRKESGDTILHQLRKLGCSLDWSRTRFTMDEVSSKAVAKAFVT
                     FFERGLIYQGVRLVNWCPRCATALSDIEVEHEDRAGKLWHIRYPFANGAGDGVVVATT
                     RPETLLGDTAVAVHPGDERYAALIGKELLLPLMNRVIPVVCDDAIDSAFGTGAVKVT"
BASE COUNT          943 a         1600 c         1663 g          863 t
ORIGIN      
        1 agcctccccg ggccggcgtc gccaaccgcc gggtcaacca acgcaccggt cccggcgtcg
       61 tcggcgtccc ggcgatccgc gtcacgaccc agcgctcaat tccgaaggcg ggccagcgga
      121 agacggcgcc cgccaccgga acgatcacgg cgccgtcccg gggatcgcgc aacaatcgta
      181 tctcatcccg ggccgcgccc acaaaccgca gtccgcccgc gggcgagacc gcgaaggtcc
      241 ggttgcccgg aagcgcggcc aagggcgaca tgcaccacag aaagagcgct ccggccgaga
      301 accccgccaa aatgaaggcc caaaacaccc gaacgagtcg gagttccaca aaggccgacc
      361 aggggccgag ggccgcggca acggcggcgt gggccccgac gccgaacacc agaaaaagcg
      421 cggcggccga cagctccagc gcccgatggt ccccgcccca cacccccgtc aacgacaccg
      481 ccaaaagcgc cagcgggtaa aagaggatcg cccaaaccca acgccagggg cgaagaaccc
      541 accgatagaa ggcgcccggt cggggcacgg gcggcgggcg aaggacggcg tcggagagcc
      601 ccgccaggca tttgtcgcac actttgtggg ccttccggtt gaaccgcccg cacttgtgac
      661 atcgccacca gggccaccgg cgaatcgccc accggacggc cgcgggcccg aggggcccga
      721 cggccgccag caccaaaaga ccaagggccg ccgcccgcaa caggcgggaa aagccgacca
      781 ggtgcccctg ggcgaaagcg tccagcaggc gaaccgcgta cgccatcatc gggcgcggcg
      841 gaaggtttta aggaggaagg cgcgggcttt ttgaaaggcc cgggcgcggt ggctgacgcg
      901 gtttttttga gcgagggtca actgcgcgaa ggtccggcgg gagcgccccg gggcgaagac
      961 cgggtcgtaa ccgaacccgt ggacgccctg gaaatcgtcg gtgatccatc ccgggcaccg
     1021 gccctcgaat atcttttccc gtccgtcggg aaacaccgcc gccaccacgg tgcgaaacac
     1081 cgcccggcgt ttggaccggg gcacgccccg gagaagccgg agcaactttc ggttgttgtc
     1141 ttcgaaagaa cagttcgggc cggcgaagcg ggcggagtag acccccggac gaccgcccag
     1201 ggccgccacg tcgaggcccg tgtcgtcgga caaggacaga agacccgtca cccgggcggc
     1261 cttgcgggcc ttgagcaggg cgttggccgc caaggttttt cccgtttcgc gcacggtgta
     1321 gggcggcggg aaatcgtcca ggccccggac gcggaacgtc gggcttttca aaatctttcg
     1381 gatttcggtc agcttgtgtc ggttgtgggt ggcgatgacg acggcgggac ggggcatggg
     1441 gcgcggggaa cggcgggatt tacgggagat cgatttcaag cccctcaaag gcggcttcga
     1501 tttccagggc ggcgccccgt tgggccacga tggaccgggc ccgttgaacc tgcaggtcca
     1561 agtccttgtc cgtgcgcagg gggtcgtggt ggaaaaggat caatttcttg actttgcccc
     1621 gaagggccat ttccaccgcg aaatcgtaaa agctgtggcc ccagccggtt tttgtgcggt
     1681 attcctcgat ggtgtattgg gcgtcgtgaa tgaggacgtc ggcgccttcc agaaaatcga
     1741 cgagcatcaa atcttcccgc tccagctcca cctcgccgcg gtcaaggtcg tcgtagatcg
     1801 gggtcttctt ggcctgttgg cgaaggaggt agcggaaggg ttcgttgtcg gtcatgtaga
     1861 ccacgcggct ttttccgttg tccacccggt agcccagggc cagcccgggg tggttgacgt
     1921 aggccggggt gacggtccag ccgttcaacg gcaagggccg ggtggtcaac tgaacgaaat
     1981 tcagcttggc ggggatttcg gagaggctca cggggaaata ttccgggctc aactggccca
     2041 cgaaaatcgt ttccagtttc cgccccgtgc cttcgcaacc gtagagggtg aaggtgttgc
     2101 cgggcgtgta caaaggccgg aaaaagggaa aaccgatgat gtggtcgaag tggaaatggc
     2161 tgatgaagag gtgggcgttg attttccccg ccgggatccg gcggcacaat tcctcgccca
     2221 gcaggcgaag gccggtgccg acgtcgaaaa cgaggagatt tttgtcgtcc gaaagctcca
     2281 cgcaggccgt gttgccgccg tagcggacgg tgtccggacc gggggtggcg atggaaccgc
     2341 gggtgcccca aaaagtgatt ttcatgatgt cgggcgaatc ctccccgggc accggtgccg
     2401 cggcccgtcc gatccgcatg gcaattattg atcaaacacg cccggttttc aaggtttttc
     2461 gaaatccccc cggacccgcc gccaacaaaa acccccttcc ccgcgaacgg ggaagggggt
     2521 ccggaagcga aaaggggcga ccgcgtttag cgggcggcca ccgccaccat tttgttcacg
     2581 atgtatttga ccagatcgac gacccggttg gaatagcccc actcgttgtc gtaccagctc
     2641 acgagcttga agaagcgctt gttgagctcg atgcccgacc cctggtcgaa gatcgacgag
     2701 gaggaggagt ggatgaaatc ggagctgacg acttcgtccg aggtcacttc caggacgcct
     2761 ttcaggtagg tctcgctcgc ctttttcatg gccgcgctga tttcggcgta gctggtgtcc
     2821 ttggtggttt taaccgtcag gtccacgacg gaaaccgtcg gcgtgggcac gcggaaggcc
     2881 atgcccgtga gtttgccttt gacctcgggg cagaccaggg cgacggcctt ggccgcgccg
     2941 gtggtcgacg ggatgatgtt ctgggccgcg gtgcgcccgc ccttccaatc cttcttggag
     3001 gggccgtcca ccgttttctg ggtggcggtg taggagtgca cggtggtcat gaggccttct
     3061 tcgatgccga agccctcttt caggagcacg tgcaccagcg gcgccaggca gttggtggtg
     3121 caggaagcgt tcgagatgat gtggtgcttg gccgggtcgt atttgttctc gttgaccccc
     3181 atgacgaccg tgagatcctc gtttttggcg ggggcggaaa tgatgacttt cttggcgccc
     3241 gcggccaggt gtcccttggc tttttcggcg tcggtgaaga ggccggtgga ttcgatgacc
     3301 aattccacgc cgaggtcttt ccaggggagg ccggaagggt ccttcgcgct cacgacgcgg
     3361 atgtcgtggc cgttgacgat caagacgtcg tcttcgactt tgtccggaga ggatttcttg
     3421 gagccgacgg tgccgtggaa acggccctgg gtcgaatcgt atttcaaaag gtaggcgagg
     3481 ttgtcggcgg ggacgatgtc gcccacggcc accacgtcca attgtttgcc caacaggccc
     3541 tgctccacca gcgcgcggaa cacgagacgc ccgatgcgcc caaatccatt gattgcaatc
     3601 ttaaccgcca taacatcctc cttaaggtag tgaaaagacg atcccggccg ggggaacggc
     3661 ccgctcaacc gcgtgaggct tcgagtcgga gagtgctttc ggggagaagc gacattttac
     3721 cacaggcgcc cccgggggtc aaccggcgga cgcccggccc cctctcaggg ggcgggcgcc
     3781 aatagggttt tgacggtctc cagaaggtcc aggggcgcca cgggtttcgc gaggaaaccg
     3841 tcggcgccgg cgtttttggc ttccaggcgg tcggccgcga acgttttggc cgtcaccatg
     3901 aggaccttgg ccttttgggt cttggcgtcg gatttgatcc ggtggcagac ttcgtaaccg
     3961 ttcacttcgg ggatcatcac gtccaggatc accaggtcgg ggggttccgc ggcgcaggcg
     4021 ttcaacccgg cctgcccgtc ccccaccgcc gacacctggt ggccagagtt ttccaacacg
     4081 aaccgaaaat actcccgcag gttgtcgtcg tcttccccca ccagaatgcg cgccatgagg
     4141 ggcctctctt tcgtcgggat tatagacctt gccccccgcg ccgtcaacgc ccgccgaccg
     4201 gaccgaaacc cccgccgaat tttgtagaat cagccccctc gctatggaac ttccgaccgt
     4261 ctacgcgccc gacgccaccg aaagcaagtg gtacgacttc tgggaaagcc gcggctactt
     4321 cgcggcccgc ccggacgccg ccaaaaaacc cttcaccgtg gtgatccccc cgcccaacgt
     4381 gaccggatcc ctccacatgg gccacgcgtt gaacaacacg ctgcaggacg tgttggtccg
     4441 ccgccggcgg atgaagggcg acaacgccct ctggattccc ggcaccgacc acggcggcat
     4501 cgccacccaa aacgtcgtcg aaaaaatgct caaaaaagag ggcaaaagcc gccacgacct
     4561 gggccgcgcc gcgtttctgg agcgcatgtg ggcctggcgg aaggaatccg gcgacaccat
     4621 tttgcaccag cttcgaaagc tcggatgctc cctggactgg tcccgcacgc gcttcaccat
     4681 ggacgaggtc agctccaagg ccgtggccaa agctttcgtc acgtttttcg agaggggcct
     4741 gatctaccaa ggcgtccgcc tggtaaactg gtgcccccgc tgcgccacgg ccctgtcgga
     4801 catcgaagtg gagcacgagg accgggcggg aaagctctgg cacatccgtt atcccttcgc
     4861 gaacggcgcg ggggacgggg tggtggtggc cacgacccgg cccgagaccc tgctggggga
     4921 caccgcggtg gccgtccacc ccggggacga gcgctacgcg gcgctgatcg gaaaagagct
     4981 gctcctcccc ctgatgaacc gggtgatccc cgtcgtgtgc gacgacgcca tcgattccgc
     5041 cttcggcacc ggggccgtca aagtgaccc
//
LOCUS       DAOQLS010000064         5055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7650_length_5055_cov_4.334200, whole genome shotgun sequence.
ACCESSION   DAOQLS010000064 DAOQLS010000000
VERSION     DAOQLS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7650_length_5055_cov_4.334200"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PK362_01940"
     CDS             complement(<1..809)
                     /locus_tag="PK362_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNC73634.1"
                     /translation="MKGNGRNLILWLLIFSGVLFFIQSLRTARVGEQEIDYSAFKQEL
                     ENGRVLEVRVRPDLLRGRIKSADGKITPFRTVPLTDPTLVAEMQDKNVKFSGEAERGW
                     LNSILVNLLWIGIFFFLWWFVIIRQMQGGGKQAMAFGRSKAKLQSGKKQKVTFGDVAG
                     CDEAKEELEEIIEFLKDPAKFQKLGGKIPKGVLLFGAPGTGKTLLAKAVAGEAGVPFF
                     SSSGSDFVEMFVGVGASRVRDLFDQGRKNAPCLLFVDEIDAVGRQRFAGIGG"
     gene            complement(806..1363)
                     /gene="hpt"
                     /locus_tag="PK362_01945"
     CDS             complement(806..1363)
                     /gene="hpt"
                     /locus_tag="PK362_01945"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNC73635.1"
                     /translation="MARHPDIQRVLLSERVLRQRVRELGRRISRDYAGRDILVVAVLR
                     GGVIFLADLIRTLSVPCALDFMAVSSYEGTTSTGAARLTLDLKKNPEGKHILLVEDVA
                     DTGLTLARLRRILKARRVASLRTCALLDKPSGRRVPLKLDYVGFRLPPGFVVGYGLDY
                     EEKYRNLPYVGLLKPSVYKRKATSK"
     gene            complement(1374..2381)
                     /gene="tilS"
                     /locus_tag="PK362_01950"
     CDS             complement(1374..2381)
                     /gene="tilS"
                     /locus_tag="PK362_01950"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNC73636.1"
                     /translation="MSDLLAEFRRRLPFPTPSRLLVACSGGPDSMALADLADRYRRET
                     GAFVAVGHVDHGLRGRAARVDAAFVERWCLSRGLPVDVQRVHGRQGARRRRKGVEEAA
                     RHFRYAALARSARKFRCPWVLTAHTLGDQAETIVLNLLRGAGPAGLAGMAADAPWPAG
                     AGPRLGRPLLGVSRDEILAYLQKRKIPWRKDATNDEPLFLRNRIRPTLRRWERWRPGF
                     FARMAQTAQIFRDEEEYWRRRLVPTREGHRRKNAPFRLDIAPFLRYPIVEQRRRLRHL
                     FGLAHFESVERVRALAAAPSGGSPRGPLDLPGVRVARAGRFLLFRYRRPGTKSGRRSL
                     Q"
     gene            complement(2387..4396)
                     /locus_tag="PK362_01955"
     CDS             complement(2387..4396)
                     /locus_tag="PK362_01955"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC73637.1"
                     /translation="MKRPSSNVWSVVGITAAVLIFYVLGFFDRMENASIDFRFARRGL
                     RPADSRIVLITINDDSLKKLGLYPWPREIHARLVDLLTRAGAEAVAFDVLFLEEDTKR
                     PDGDRALGRAASANGRVVFGGLFTQTSDGEPKMMDRPIATLRRSGIGTGFVNVFPEKD
                     GILRSGALWVPFEDRVMPSLSLAALSVVRQRPVESLARAFEEPTAGDWHELMINFTGW
                     KEDAEGSPSPYRYYSYADVLSGRTDPDAFKNKIVIVCGTATGLFDTTAVPNLRNFPGP
                     EIHANLIDNLLNGTYLRAPPVWVSLPVILLLGLAVGPWLAGLKTKRGAAAVFAILIGY
                     YALCQFGFNRFYYVFNFVVPLVALLASYGTMLFRRLLTEEKEKRRIKSMFGQYVSPKV
                     IDILIRDSSRMKLGGEERHVTLFFSDIAGFTTVSEKLTPQELVEVLNDYLSTMTDVIL
                     DLDGTLDKFIGDAIMAFWNAPLDQPKHALLACRAAVGQMALLPELHRRFGDKGWPIVE
                     FRIGLNTGPAVIGNMGSSTHRNFTAVGDTVNLASRLEGANKMFGTRILISESTLQAAK
                     DDIEVRELDFLRVKGKTQPIRVYELLALKNGVSPEKKKTVSLFTEGLALYRDRRFTDA
                     TAKFFESLKTSPDDKPSQIYIKRCEQFLAAPPPADWDGVYVMTSK"
     gene            complement(4393..>5055)
                     /locus_tag="PK362_01960"
     CDS             complement(4393..>5055)
                     /locus_tag="PK362_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNC73638.1"
                     /translation="PEEDGVLRRSALWLEFEGEKIPSLALAALAAARRESPNKMIRQL
                     SGGVPGRTPLINFSGWNSGRGGSRSPYHYHSYADVLQGRLDPGVFNDKIVLVGGTAMG
                     LFDSLTVPNLPVFPGLEIHANIVDNLLNHSFLWSPPRWGTWGIVFLFQMVLGTWLAGL
                     TTSRGTTIVLGGVVGYYFLAQLVFNRWSLALDVVAPMATTLANHGTLLFRRLLAYERE
                     IA"
BASE COUNT         1026 a         1498 c         1644 g          887 t
ORIGIN      
        1 ccgccgatgc cggcgaagcg ctggcggccc acggcgtcga tttcgtccac gaagagcagg
       61 cagggcgcgt tcttgcgtcc ttgatcaaag agatcccgaa cgcggctggc gcccacgccg
      121 acgaacattt ccacgaagtc cgagcccgag ctggagaaaa agggaacgcc ggcctcgccc
      181 gccacggcct tggccaggag ggttttgccc gtgccgggcg ccccgaacag gagaacgccc
      241 ttggggattt ttccgcccaa tttttgaaat ttagcggggt ctttcaggaa ttcgatgatt
      301 tcctcaagct cttctttggc ttcgtcgcag ccggccacgt cgccgaaagt gaccttctgc
      361 tttttaccgg actgaagctt ggccttggaa cggccgaagg ccatggcctg ttttcccccg
      421 ccctgcatct gacggatgat cacgaaccac cagaggaaga agaaaatgcc gatccacaac
      481 agattgacca ggatggaatt cagccatccc cgctccgctt ctcccgaaaa tttgacgttt
      541 ttgtcctgca tttcggccac gagggtcgga tcggtcaggg gcacggtgcg gaaaggcgtg
      601 attttgccgt cggcggattt gatccggccc cgaaggaggt cggggcggac gcgcacctcc
      661 agaacgcgcc cgttttccag ctcctgttta aaggcggagt aatcaatctc ctgttccccc
      721 acccgggcgg tgcggaggct ttggatgaaa aagaggaccc cggagaaaat caggagccag
      781 aggatgaggt ttcggccgtt gcctttcatt tggaggtcgc ctttcgcttg taaacgctcg
      841 gtttcaacag ccccacgtag ggcagattcc ggtacttttc ttcgtaatcc aatccgtacc
      901 cgaccacaaa ccccggggga agccgaaagc ccacgtagtc gagctttaag gggacgcgtc
      961 gccccgaggg tttgtccaac aaggcgcagg tccgaaggga cgccacgcgc cgggccttca
     1021 aaatccgccg aaggcgggcc agggtcaggc ccgtgtcggc cacgtcttcc accagaagaa
     1081 tgtgcttgcc ctcggggttc ttcttcaaat cgagggtgag ccgggccgcc ccggtggacg
     1141 tggtgccttc gtaggaactg acggccatga agtccagggc gcaaggcacc gaaagcgttc
     1201 ggatcaaatc ggccaaaaag atcacgccgc cccgcaggac ggcgaccacc aggatgtccc
     1261 ggcccgcgta atcccgggaa atgcggcggc cgagttcccg aacccgttgt cggagaaccc
     1321 gttccgaaag caggacgcgt tgtatatcgg gatggcgggc catgggtcga accttattgt
     1381 aacgaacggc ggccgctttt ggttcccggg cggcggtagc gaaagaggag gaatcggccc
     1441 gcgcgagcga cgcggacgcc cggaaggtcc aaggggccgc ggggggaccc gccggagggc
     1501 gcggccgcca gggcccgaac ccgctccacg gattcgaaat gggccagccc gaaaagatga
     1561 cggaggcgcc gccgctgttc aacgatagga tacctcaaaa aaggggcgat atcaagacga
     1621 aacggggcgt ttttccgtcg atggccttcg cgggtgggga cgaggcgccg ccgccaatat
     1681 tcctcctcgt cccggaagat ttgggccgtt tgggccatcc gggcaaaaaa acccgggcgc
     1741 caacgctccc atcggcgaag ggtcggacga atgcgatttc gcaggaacag gggttcgtcg
     1801 ttggtggcgt ccttccgcca ggggattttc cttttttgga ggtaggcaag gatttcgtcc
     1861 cgggagaccc cgagcagggg gcgtcccagc cggggcccgg cccccgcggg ccagggagcg
     1921 tcggcggcca tgcccgccaa tccggccgga ccggcgccgc ggagcaaatt cagcacgatg
     1981 gtttcggcct gatcgccgag cgtgtgggcc gtgaggaccc acgggcaccg aaacttccgg
     2041 gcggaccggg ccaacgcggc gtagcggaaa tggcgcgcgg cctcttccac gccttttcgg
     2101 cgccttcgcg ccccttgccg accatggacc cgctgcacgt ccacgggcaa tccccgggaa
     2161 aggcaccacc gttccacaaa ggcggcgtcg acccgggccg ctctcccccg caacccgtgg
     2221 tccacgtggc cgacggcgac aaaagccccc gtttcgcgac ggtaacgatc cgccaggtct
     2281 gccaaggcca tggaatccgg gccgccggaa caggcgacga ggaggcggga aggcgtcggg
     2341 aaaggcaggc gacggcgaaa ttcggcgagg aggtcggaca acgcggttac ttgctggtca
     2401 tcacgtaaac cccgtcccaa tcggcgggcg ggggcgcggc gaggaattgt tcgcaacgct
     2461 tgatgtaaat ctgggacggt ttgtcatcgg gcgatgtttt cagcgattcg aaaaatttgg
     2521 ccgtggcgtc cgtgaatcgg cgatcccgat agagcgccag gccctccgtg aacagggaaa
     2581 cggttttttt cttttcgggg gagaccccgt ttttcaaggc cagcaattcg tacacccgga
     2641 tgggttgagt cttccccttc acccgcagga aatccaactc ccgaacctcg atgtcgtctt
     2701 tggccgcctg gagcgtgctt tcggaaatca ggatccgggt gccgaacatt ttattggccc
     2761 cttccaggcg ggaggccaga ttgacggtgt cgcccacggc ggtgaagttc cggtgggtgg
     2821 aggagcccat gttgccgatg acggccgggc cggtgttcag cccgatgcgg aattccacga
     2881 tgggccagcc tttgtcgccg aaacggcggt ggagctcggg caatagcgcc atttggccga
     2941 cggcggcccg gcaggccagg agggcgtgct tcggctggtc caacggcgcg ttccagaaag
     3001 ccatgatggc gtcgccgatg aacttgtcga gggttccgtc gaggtccaaa atcacgtcgg
     3061 tcatggtgct caggtaatcg ttcagcacct ccaccagttc ctggggcgtc agtttttcgg
     3121 agaccgtggt gaaaccggcg atgtccgaaa aaaacaaggt gacgtggcgc tcttcccccc
     3181 ccaacttcat gcgggaggag tcccggatca ggatatcgat cactttcggg gagacgtatt
     3241 ggccgaacat gcttttaatc cgccgttttt ccttttcctc ggtcaaaagc cggcggaaca
     3301 acatggtccc gtaactggcc aacaaggcca ccaggggcac gacgaaatta aaaacgtagt
     3361 aaaaacggtt gaacccgaat tggcagagag cgtagtagcc gattaaaatc gcgaacacgg
     3421 cggcggcccc gcgtttggtt ttgagaccgg cgagccaggg cccgaccgcg aggccgagga
     3481 ggagaatcac gggaagggac acccacaccg gcggggcgcg aagatacgtc ccgttgagca
     3541 ggttgtcgat cagattggcg tgaatttcgg ggccggggaa attgcgcaga ttgggaacag
     3601 cggtggtgtc gaaaagtccc gtggcggttc cgcagacgat gacgatttta tttttaaagg
     3661 cgtccgggtc ggtccggccc gacagcacgt ccgcgtagga gtaataccgg tacggggagg
     3721 gagacccttc ggcgtcttct ttccagcccg tgaaattaat catcaattcg tgccaatccc
     3781 cggccgtggg ctcctcaaag gcccgggcca gactttccac cgggcgctgg cggacgacgg
     3841 agagggcggc caagctcagg gagggcatga cgcggtcttc gaaaggaacc cacagggcgc
     3901 cgcttcgcaa aatgccgtcc ttctcgggga aaacgttgac gaatccggtg ccgatgccgg
     3961 accggcggag ggtcgcaatg gggcggtcca tcattttggg ttccccatcg gaggtttggg
     4021 taaataggcc gccgaaaacc acccggccgt tggccgacgc ggcgcggccc agggcgcggt
     4081 cgccgtccgg gcgcttggtg tcttcctcga gaaacaaaac gtcgaaggcg acggcttcgg
     4141 ctcccgctcg ggtaagaagg tcgaccaacc gggcgtgtat ttcccgcggc caggggtaga
     4201 gccccaactt ttttagggaa tcgtcgttga tcgtgatcaa aacgattcgg gaatcggcgg
     4261 ggcgaagacc gcgtcgggcg aaacggaaat cgatggaggc gttttccatc cggtcgaaaa
     4321 agcctaaaac gtaaaaaatc agcaccgcgg cggtgatgcc gacgaccgac caaacgttgg
     4381 aggatgggcg tttcatgcga tctcccgttc gtacgccaga agccggcgga acaacagcgt
     4441 gccgtggttg gccagcgtcg tcgccatcgg ggcgaccacg tccaaggcca gcgaccagcg
     4501 gttaaaaacc agctgggcaa ggaaataata gcccacaacg cctccgagga caatcgtcgt
     4561 tccgcgcgag gtcgtcagcc ccgccagcca cgtccccagg accatctgaa agaggaaaac
     4621 gattccccag gtgccccaac ggggcggcga ccaaagaaac gaatggttca acaaattatc
     4681 aacgatgttg gcgtggattt ccagtcccgg gaaaacgggg aggttgggga ccgtcagcga
     4741 gtcaaacagt cccatggcgg tgcctcccac caggacaatt ttgtcattaa acacgccggg
     4801 gtccaggcgg ccctggagaa cgtcggcgta ggaatggtag tggtacggcg accgacttcc
     4861 gccccgtccg ctgttccagc ccgagaaatt gatgaggggg gtccggcccg ggacgccccc
     4921 ggacaattgg cggatcattt tgttcggaga ttcccgccgg gcggcggcca gggcggccag
     4981 ggcgagagag gggatttttt ccccttcgaa ttccaaccac agggcgctcc gtcgaaggac
     5041 gccgtcctcc tcggg
//
LOCUS       DAOQLS010000065         5051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7657_length_5051_cov_3.999199, whole genome shotgun sequence.
ACCESSION   DAOQLS010000065 DAOQLS010000000
VERSION     DAOQLS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7657_length_5051_cov_3.999199"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PK362_01965"
     CDS             <1..941
                     /locus_tag="PK362_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNC73639.1"
                     /translation="RALAETLPRLTSEVNRQMEAVSRQVLEGQKSVGDRLDNAARVVG
                     EVQKNLGALGQASEKIFEVGKDIAGLQDILRAPKLRGGLGEYFLTDLLSQILPARNVT
                     LQYAFRSRETVDAVIRLGERLVPVDAKFPLENFRKVLEAKSEEDRLALRKKFTQDVRK
                     HVDAIAAKYILPDEGTYDFALMYIPAENVYYECIVKDDNAGSDGALVEYALKRRVVPV
                     SPGSFYAYLQAIAMGLRGFQIEENARRIMDDLGRLRVDFERFAEEFEVLGKHLSNAKV
                     KHDDAGRRLDRFQEKLERVQDAPALTKDPSEPAALP"
     gene            complement(1025..5035)
                     /locus_tag="PK362_01970"
     CDS             complement(1025..5035)
                     /locus_tag="PK362_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73640.1"
                     /translation="MFRRNTNGENRPNAVFVDLGLKFRRSGDPAIAAADKAAAALQKN
                     PLAGQRPSVFISDLIRHIGSGQNIGVIFEAVLEHFGTTDGLKFLNSVSGLESDDQAKL
                     DQFIAATNRRLRAESMAGGLGVSPAATIPGLVPLLAWAYGLDKNNPADLNLIVNEKAP
                     LVESIVTGAFFYAGVGLISALGSFFGVSIDHSFFAATGLFALVNVAFGFSHRSVYRFA
                     PPEPGTHGLGRWKLESAPASRQTRWNLALRGMAIHAPLLLAFMDPFGGLFLAPLGALA
                     GVVLHVVHNRAAARFGWTALTAGHAPTAGPAAPKYIDAEGKAVLARAGAKLRASVEKL
                     RRGGRLTEDDHVTGMFICSNNESRSAISEILARKALFNFNSSLVPAAERDQFQSSINA
                     ILPRLEVTSPEGTVTTLTMRSGMKLDFPEGTQYKEVLPEAPDPEALKTIRFFSAGLGA
                     NGFGRTRHYRAEALKANVSAVPPEVYVPPATTKFDENTLRARKPDFLIAFVDTKALRP
                     DGRPAATALAVLKAQAKKLGFEDKLVIVEMPDEEPDTLWPKLTRFIQTEVLTGLGFSA
                     TKPAAAAPAHGPTRGAVGWWAPVANRLLDRFPSLANSRNLSFALFGALESVLLLAVNF
                     LLPFTGYAEGSDLLNDATLGVALFGAHYVSGILNFNPETKRFEPAPGTFGRALGATAI
                     ASTTFVPLMMLDLFMTGGVSHWMTAAAGAAGVAAHALLNVRDSIVRWMGDRTRSVKTL
                     ATAALLSLNLLSVPLALSQTNVETPALPLATLERAQVARLNMSQLAPGEASMKRAEQW
                     MAKDNPLLQASAQMQRIKLEGANTPDDLKILTWLLEVTHTWKLDTSKVSAVVLTSNLP
                     APGMFNDLKKKGESDAEAKRVVYLDRNNTALGLLATWAHEAGHITRDNRQVAEADTLG
                     EDSRIAASLLPYINDAAKDWPGVRDRFGLPADLAFYNPQAMGVLGGTNLSARDAFWLR
                     YDVVPAVAAFLLREAPTPALLSPNHSAVKAEINRLVPLVSQRVFRVQAVYGLSTYSIG
                     LTNGRVTITFMDARNRAFPIDLGIPAAPKTPEPGLPAGTGSTQGPNGRRLPGTGEEDF
                     NAWARLALMETLLGSKAEPFEFTIPTPVGSGDYWTARNFNELRSEYTSVDDATRLGGF
                     ESFLQLLSNDRTGARRWINEPARREQLAEGALLAAGARAAQDSATTLHVNLFGYKGWS
                     AREQKDMRIFLTGLAGAVRPGTNLVVAAEGANPLADVFAEAIKTGAAAVHVLTPARVP
                     GLADAEGRVVMSEVVAWINRPENQKSGMMKGTRIIARDANSVTKAEGFLIIGLQKLSE
                     AIANDIATMLKIMTQA"
BASE COUNT          933 a         1515 c         1683 g          920 t
ORIGIN      
        1 cccgggccct cgccgaaacc cttccccgcc tcacctccga ggtcaaccgg cagatggagg
       61 cggtctcccg gcaggtgttg gaggggcaga aatccgtggg ggaccgtttg gacaacgccg
      121 cccgggtggt gggggaggtt caaaagaatc tgggcgccct gggacaggcc tccgaaaaga
      181 tttttgaagt gggaaaagac atcgccggcc tccaggacat cctgcgggcg ccgaagcttc
      241 ggggcggact gggagaatat ttcctcaccg atcttttgtc gcaaatcctt ccggcccgaa
      301 acgtcaccct ccagtacgcc ttccgctccc gggaaaccgt ggacgccgtc atccgcctcg
      361 gggagcgcct ggtccccgtg gacgccaaat ttcccctgga aaacttccgc aaggtgttgg
      421 aggccaaatc ggaagaggac cgcctcgccc ttcgaaagaa gttcacccag gacgtgcgca
      481 aacacgtgga cgccatcgcc gccaaataca tcctgccgga cgaaggcacc tacgatttcg
      541 ccttgatgta catcccggcc gaaaacgttt attacgagtg catcgtgaag gacgacaacg
      601 ccgggtccga cggggccctg gtggaatacg ccctgaaacg gcgggtggtt ccggtgtccc
      661 cgggaagctt ctacgcctat cttcaggcca tcgccatggg gttgcggggg tttcagatcg
      721 aagagaacgc ccgccgcatc atggacgacc tggggcgcct gcgggtggat tttgaacgat
      781 ttgccgagga attcgaggtg ttgggcaagc acctgtccaa cgccaaagtg aagcacgacg
      841 acgcgggccg gcgattggac cgttttcagg aaaaactgga acgggtgcag gacgcccccg
      901 cgttgaccaa agacccctcc gaacccgccg cccttccgta acgcaacctc ccaaccccca
      961 aaaaaatccc cgcggagggc cgcggggatc gctttgaatc tttgtgtttc gtggtcgagg
     1021 cgaattacgc ctgggtcatg atcttgagca tcgtcgcaat gtcgttggcg atggcctcgg
     1081 acagcttttg caaaccgatg atcaggaagc cttcggcttt ggtgacgctg ttggcgtccc
     1141 gggcgatgat ccgggtgccc ttcatcattc ccgatttctg gttttccggc cggttgatcc
     1201 aggccaccac ttcggacatg accacccggc cctcggcgtc cgccagcccc ggcacccgcg
     1261 ctggggtcag cacatggacg gcggccgcgc cggtttttat ggcctcggcg aagacatcgg
     1321 ccagggggtt ggctccttcg gccgcgacga ctaaattcgt tcccgggcgc accgcgccgg
     1381 ccaggcccgt gaggaaaatc cgcatgtcct tctgttcccg ggcgctccag cccttgtagc
     1441 cgaacaaatt gacgtgaagg gtggtggccg aatcctgggc cgcccgggcg cccgcggcca
     1501 agagggcccc ttcggccaat tgctcccgac gggcgggttc gttgatccac cgccgggcgc
     1561 cggtccggtc gttggaaaga agttggagga agctttcaaa accgcccagg cgcgtggcgt
     1621 cgtccaccga cgtgtattcg gaacggagtt cgttgaagtt ccgcgcggtc cagtagtcgc
     1681 cgctcccgac cggggtcgga atcgtaaatt cgaagggttc cgccttggaa cccaacagcg
     1741 tttccataag ggccaatcgg gcccaggcgt tgaagtcttc ttcgccggtc ccgggaagcc
     1801 gccgaccgtt gggcccttgg gtcgaccccg ttcccgcggg caatcccggt tccggggttt
     1861 tgggcgcggc cggaatgccg agatcgatgg gaaaggcgcg gttgcgggcg tccatgaagg
     1921 tgatcgtgac ccggccgttg gtgaggccga tgctgtaggt ggacaatcca tagaccgcct
     1981 ggacccggaa cacccgctgg ctcacgaggg gaaccagccg gttgatttcc gccttgacgg
     2041 cgctgtggtt ggggctcagg agcgccgggg tcggggcctc ccgcagcagg aaggccgcga
     2101 cggcgggaac cacgtcgtaa cgcagccaga acgcgtcccg ggccgaaagg ttggtgccgc
     2161 ccaacactcc catggcctgg ggattgtaaa aggccaggtc ggccggcaat ccaaatcggt
     2221 cccgaacccc cggccaatcc ttggccgcgt cgttaatata gggcaggaga gaggcggcga
     2281 tgcgggagtc ctcccccagg gtgtcggctt cggccacctg gcgattgtcc cgggtgatgt
     2341 ggccggcttc gtgggcccag gtcgccaaca ggcccagggc cgtgttgttg cggtccaagt
     2401 agaccacccg cttggcctcg gcgtcggatt cgcctttctt ttttaaatcg ttgaacattc
     2461 ccggggccgg taaattggaa gtgaggacca cggcgctgac tttcgaggtg tccagtttcc
     2521 aggtgtgggt cacttccaac agccatgtca aaatctttaa gtcgtccggg gtgttcgccc
     2581 cctccagttt aatccgctgc atttgggccg acgcttgcag gagcggattg tccttcgcca
     2641 tccattgttc ggcccgcttc atcgacgcct ccccgggcgc caattgggac atgttcaggc
     2701 gggccacctg ggcccgttcg agcgtcgcca ggggcagggc cggggtctcg acgttggttt
     2761 gggaaagagc caagggaacg ctcaggaggt tgagggacag gagggccgcg gtggccagcg
     2821 ttttcaccga gcgcgttcgg tcgcccatcc agcgaacgat ggaatcccgc acattgagaa
     2881 gcgcgtgggc cgcgacgccg gcggcgcccg ccgccgcggt catccaatgg gacacgccgc
     2941 cggtcatgaa caggtccagc atcatcagcg gaacaaaggt ggtcgaggcg atggcggtcg
     3001 ctcccagggc ccgcccaaag gtgccgggcg cgggttcaaa gcgtttcgtt tccggattga
     3061 agttcagaat cccgctcacg taatgcgcgc cgaacagggc gacgccgagc gtggcgtcgt
     3121 tcaataggtc ggagccttcg gcgtagcctg tgaaggggag aaggaagttc accgccaaaa
     3181 gcagcaccga ttccaaggca ccgaagaggg cgaaagagag gttccgcgaa ttcgccaggg
     3241 aggggaaccg gtccaggagc cgattcgcca cgggcgccca ccaccccacg gcgccccggg
     3301 tcgggccgtg ggcgggggcc gcggcggccg gtttggtggc cgagaatcca agtccggtca
     3361 acacttcggt ttgaatgaac cgggtcaatt tcggccaaag ggtgtccggc tcttcgtcag
     3421 gcatttcaac aatcacgagc ttgtcttcaa agccgagttt tttggcctgg gctttcaaaa
     3481 ccgccagggc tgtggccgcg ggtcgtccgt ccggccgaag ggccttggtg tcgacgaagg
     3541 cgatcaggaa gtcgggtttg cgggcgcgca gggtgttctc gtcaaatttg gtcgtggcgg
     3601 gcggaacata cacttccggg ggcacggccg acacgttggc tttcagggcc tcggcgcggt
     3661 aatggcgcgt ccgtccgaac ccgttggccc ccaggccggc ggagaagaat cgaatcgtct
     3721 tcagggcttc ggggtccggg gcctcgggca ggacttcctt gtactgcgtg ccttccggga
     3781 agtccagctt catcccgctg cgcatggtca gggtcgtgac ggtcccttcg ggggacgtca
     3841 cttccaaccg gggcagaatg gcgttgatgg aggattgaaa ctgatccctc tcggcggccg
     3901 ggaccaggga actgttgaaa ttgaagagcg ctttgcgcgc caggatctcc gatatggcgc
     3961 ttcggctttc gttgttggag caaatgaaca tccccgtgac gtgatcgtcc tcggtgagcc
     4021 gaccgccgcg gcgcaatttt tcaacgcttg cccggagttt cgcgcccgcc cgggccagga
     4081 ccgctttgcc ttccgcgtcg atgtatttgg gggccgccgg accggcggtg ggcgcgtgtc
     4141 cggcggtcaa ggccgtccaa ccgaaacggg cggcggcgcg gttgtgaacg acgtgcagga
     4201 ccacgccggc cagggcgccc aggggggcca ggaacagccc gccgaaggga tccatgaagg
     4261 ccaggagcaa aggcgcgtga atggccatgc cccgcagggc caggttccac cgggtttgcc
     4321 gggaggccgg ggcggactcc aatttccatc ggcccaaccc gtgggtcccc ggttccgggg
     4381 gcgcaaatcg ataaacggaa cggtgcgaaa acccaaaagc gacgttgacg agggcgaaga
     4441 ggccggtggc ggcgaagaag gaatggtcga tggaaacccc gaagaaggaa cccaaggcgc
     4501 ttatcaagcc gacgccggcg taaaagaaag cgccggtgac gatcgattcc accagggggg
     4561 ccttttcgtt gacgatgagg ttgaggtcgg ccggattgtt cttgtcgagg ccgtaggccc
     4621 aggcgaggag gggcaccagt cccggaatcg tcgcggccgg tgaaactccc aaacctcccg
     4681 ccatggactc cgcgcgaagg cggcgattgg tggcggcaat aaattggtcc agcttggctt
     4741 gatcgtcact ctccaagcct gaaacagaat tcagaaactt aagtccgtcc gtcgttccga
     4801 aatgctcaag aacggcttca aagatgacgc cgatgttttg gccactaccg atgtggcgga
     4861 tcaagtccga aataaatacc gatgggcgct gacccgccaa ggggtttttt tgcaaagccg
     4921 cggcggcttt gtccgcggcg gcgatggcgg gatcaccgga ccgtcggaat ttaagtccca
     4981 agtcgacaaa tacggcgttt ggccggtttt cgccgttggt gttacggcga aacatgaagt
     5041 tgtccgggtt g
//
LOCUS       DAOQLS010000066         5028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7712_length_5028_cov_5.140358, whole genome shotgun sequence.
ACCESSION   DAOQLS010000066 DAOQLS010000000
VERSION     DAOQLS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7712_length_5028_cov_5.140358"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK362_01975"
     CDS             <1..284
                     /locus_tag="PK362_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73641.1"
                     /translation="ALKNTPHAKLAVAVLHGGALKGLGKEDFFFTRNPAEAVAHSKKT
                     DGWSFFLAPNTVQEVLDVSTAGMVMPPKSTYFYPKIPSGLLSHALKGSL"
     gene            complement(288..704)
                     /locus_tag="PK362_01980"
     CDS             complement(288..704)
                     /locus_tag="PK362_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73642.1"
                     /translation="MIELMLVVAIIALLAAIAIPKFADLVVKAKEAAVRGKLGTVRSL
                     ISIYYADNEGRYPWLSGYMEWVFVPKYAETIPGISVPGVPSHAEDNGFIPFAVMIDGP
                     WSPLSVQKAWGYNSQNGSVLVNCSHTDSKATTWYVW"
     gene            complement(725..1201)
                     /locus_tag="PK362_01985"
     CDS             complement(725..1201)
                     /locus_tag="PK362_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73643.1"
                     /translation="MIELMLVVAIIGLLAAIAIPKFANLVTKSKEAGVKGKLGALRSA
                     VNLYYVNNEGNFPSCLGVAAGTFCLPLVLVPNYIDKIPPIALPLPDHPENSGVRFIQP
                     TGPGYGMIPEWRAYLGPGGEGGWYYQMIPWFGSFDKERVQVNCTHTDSNGTPWSVW"
     gene            complement(1417..1614)
                     /locus_tag="PK362_01990"
     CDS             complement(1417..1614)
                     /locus_tag="PK362_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC73644.1"
                     /translation="MKGTVKWFNASKGYGFITPESGEDVFVHFSAIQSEGYKTLEEGQ
                     AVEFEVTKGPKGLQASNVRTA"
     gene            complement(1793..2938)
                     /locus_tag="PK362_01995"
     CDS             complement(1793..2938)
                     /locus_tag="PK362_01995"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNC73645.1"
                     /translation="MTAVVPPGSAVGVLGSGQLGRMFALAARRLGYRVHTFSPESDTP
                     TGQVADVEITASYDDLDAVRKFAKGVGVVTFEFENVPAACVAAAQEFAPVRPNPEALR
                     VSQHRLREKTYLQEKGFPVAAFRHVRSLEELQSALEALGAPAVLKTVGFGYDGKGQRV
                     IRSAADAPAAFAALGGQEAVLESFVDFACELSVVVARSANGATAHWGAIENLHANHIL
                     DVSVSPAITPGPGQAQQAVEIALDVLKTLDIVGVLCVEFFLTKKGRLLINELAPRPHN
                     SGHLTLDASVTNQFEQQLRAVCGLPLGATDFFAPAAMANLLGDVWAGGEPVWARALAF
                     PDVKLHLYGKADARPGRKMGHLTALARTPVEARDLVLKARRALAPAP"
     gene            complement(2935..3396)
                     /gene="purE"
                     /locus_tag="PK362_02000"
     CDS             complement(2935..3396)
                     /gene="purE"
                     /locus_tag="PK362_02000"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNC73646.1"
                     /translation="MGSTSDWETMRHAAQVLADFGVPHEKKVVSAHRTPKLLASFADR
                     AEARGLEVIIAGAGGAAHLPGMVAAQTVLPVLGVPVESRALKGLDSLLSIAQMPAGIP
                     VGSLAVGKAGATNAGLLAVAILAGSRPDLRRKLHQFRRKQTATVLQTPLPR"
     gene            3618..4274
                     /locus_tag="PK362_02005"
     CDS             3618..4274
                     /locus_tag="PK362_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC73647.1"
                     /translation="MFCALNADAVKKLDGAQTENLFKKGQILFYEGNPAVGVYCVSAG
                     EVKLSKTGAGGRPQILGIVGPGTLMGHRSLLTNQPHTMTAEALVDSRVCFFEKKFFLD
                     LLNQEPSVSIEVLKKLSQDLDAVEDRLLDLVEKPVPVRLARLLLMLKEVHGRPSPKGV
                     VLTLGLTREEMAEMIGTTQETTIRLLSRFKQKGYLRLDKRTIVLADIPGLLKVARLSL
                     "
     gene            4373..>5028
                     /locus_tag="PK362_02010"
     CDS             4373..>5028
                     /locus_tag="PK362_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNC73648.1"
                     /translation="MDPSLLSTGGAPAPDTAPWHAWSVERTRTALGAPAVGLSESDAA
                     GRLARDGPNRLSPPVRRSPSRLFLAQFNDFMIRVLLGAALVAGLLGDRGDAAAILAIV
                     LLNAGVGFVQEFRAERALDALREIDAPTARVERDGRRRLINADGLVRGDRVILEAGAR
                     VPADLRLSEAQTLRIDESALTGESAPVEKNTDPLPIKTEMADRTNMAYRGTLVVHGRG
                     A"
BASE COUNT          959 a         1583 c         1554 g          932 t
ORIGIN      
        1 ccgccttaaa aaacacgccc cacgccaagc tggcggtggc cgtgcttcat gggggcgcgc
       61 tgaaggggct ggggaaggag gatttctttt tcacccggaa tcccgccgag gccgtggccc
      121 attccaagaa aaccgacggt tggtctttct tcctggcccc caacaccgtg caggaagtgt
      181 tggacgtttc gacggccggc atggtcatgc cgcccaaaag cacgtacttt taccccaaaa
      241 tcccctcggg gcttctcagc cacgccctta agggttccct ctaaaaatca ccagacgtac
      301 caggtggtgg ccttcgaatc ggtgtgactg caatttacca gtacggagcc attctgcgag
      361 ttgtaccccc acgccttttg aaccgataga ggggaccatg gaccgtcaat catcacggcg
      421 aacggaataa atccattgtc ctcggcgtgg gacggaactc cggggacgga gatgccggga
      481 atggtctcgg catattttgg aacgaacacc cattccatgt aaccgctgag ccaaggatag
      541 cggccctcgt tgtcggcgta ataaatgctg atcaagcttc gcacggtccc cagttttccc
      601 cgaaccgccg cctccttcgc tttgaccact aaatcggcaa attttggaat ggcaatcgcg
      661 gcgaggaggg cgatgatggc cacgaccagc atcaattcga tcaacgtaaa gccccgaccc
      721 cttctcacca tacgctccag ggcgtgccgt tgctgtcggt gtgggtgcaa ttgacctgga
      781 cccgttcctt gtcgaaggaa ccgaaccagg gaatcatttg atagtaccac ccgccttcgc
      841 ccccggggcc caaatacgcg cgccattcgg gaatcatccc gtatcccggg cccgtcggtt
      901 gaatgaagcg caccccggaa ttttccggat ggtccggaag ggggagggcg atggggggaa
      961 ttttgtcgat gtaatttggg accagaacca acggcaggca gaaggtgccg gccgcgactc
     1021 cgaggcaact tggaaaattc ccctcgttgt tcacgtaata gagattcacc gcgcttcgca
     1081 aggcccccaa tttgcccttt acccccgcct ccttcgattt cgtcaccaga ttggcgaatt
     1141 tggggatggc gatggcggcg agaaggccga tgatggccac caccagcatc aattcgatca
     1201 aggtaaaggc ccgcggatta tttattgaaa tcatggccct tgagtataaa gccgggaaag
     1261 taatatctca acgggggaca cccggttttt taatttcgaa ttttagcgag gctttaattt
     1321 aaaagcggga aaccgaatat tgaagcagcg ggacaaaaat tgaacccccc gtcccgcgct
     1381 gatcgcggaa cggggggttc tgtcgaacgg gtcgggttac gccgtgcgaa cgttggaggc
     1441 ctgaagacct ttggggcctt tcgtcacctc gaactcgaca gcctgtccct cttccaacgt
     1501 cttatagccc tccgactgga tggcggagaa gtggacgaaa acgtcctctc cgctctccgg
     1561 cgtgatgaag ccataaccct tggaagcgtt gaaccatttc actgtgcctt tcattgtgtt
     1621 gctcaccgcc cttacgtaag tctaaattag agccggaccc gttcgccgtg ggctactaca
     1681 aaaaaaaccg cgcgggaatt aatcccgtgc ggtcccttca gcgtgtgttt cgcagtgcga
     1741 catcagtcat actcaactta cttgattaag tataccaccg cgccccaaaa agtcaagggg
     1801 cgggggcgag ggcccgtcgg gccttaagaa ccaggtcccg agcttccacc ggtgttcggg
     1861 cgagggccgt caggtggccc attttccgac cgggccgggc gtcggccttt ccgtaaaggt
     1921 gaagtttcac gtcggggaag gccagggccc gggcccagac gggttctccg ccggcccaga
     1981 cgtcccccag caaattggcc atggccgcgg gggcgaaaaa gtccgtggcc cccaggggca
     2041 agccgcagac cgcccgcaat tgttgttcga attggttcgt gaccgaggcg tccaaggtca
     2101 aatgccccga attgtggggc cggggcgcga gctcattgat taaaagccgg ccctttttcg
     2161 ttaaaaagaa ttccacgcag agcacgccga cgatgtccag ggttttcaaa acgtccaggg
     2221 cgatttccac cgcctgttgc gcctgccccg ggccgggggt gatggccggg gacaccgaca
     2281 cgtccaaaat atggttggcg tggaggttct cgatggcccc ccagtgggcc gtagcgccgt
     2341 tggcgctccg ggccaccacg acagataatt cgcaggcgaa atccacgaag gattccaata
     2401 ccgcttcctg accgcccagg gcggcgaagg ccgccggggc gtccgcggcc gaacggatca
     2461 cccgctgacc tttgccgtcg tagccgaacc cgacggtctt gagcaccgcg ggcgctccca
     2521 gggcctcaag ggcgctttgt agttcctcga gcgagcgcac gtggcggaag gcggccacgg
     2581 ggaagccttt ttcctggagg tacgtctttt cccgaaggcg gtgttgggaa acccgcaggg
     2641 cctcggggtt gggccgcacg ggggcgaatt cctgggccgc cgccacgcag gcggcgggga
     2701 cgttttcaaa ttcaaaggtg acgaccccga cgcccttggc gaattttcga acggcgtcca
     2761 gatcgtcgta ggaagcggtg atttccacgt cggccacctg gcccgtgggg gtgtccgatt
     2821 cgggggaaaa ggtgtgaacc cggtacccca ggcgccgggc ggccagggcg aacatgcggc
     2881 ccaattggcc ggacccgagg acgccgacgg cggaccccgg cggaacgacg gccgtcatcg
     2941 gggcagcggg gtctgaagga ccgtggcggt ttgtttccgc cggaattggt ggagcttgcg
     3001 gcgaaggtcc gggcgggagc cggccaaaat ggccacggcg agaagccccg cgttggtggc
     3061 gccggccttg ccgacggcca agctgccgac gggaatgccc gccggcatct gggcgatgga
     3121 gaggagggag tccaggccct tcagggcccg gctttccacg ggaacgccca ggacgggcaa
     3181 aacggtttgg gccgccacca tgccgggcag gtgcgcggcg ccccccgccc cggcgatgat
     3241 gacctcgagg ccccgggctt cggcccggtc ggcgaaggac gccaggagtt tgggggtgcg
     3301 gtgggcgctc accacttttt tctcgtgggg cacgccgaaa tccgccagga cctgcgcggc
     3361 gtggcgcatg gtttcccagt ccgacgtgct gcccatgatg acggccacca aaggtcgaat
     3421 cacgacgcca ttgtagaaaa ttgttcctcc gggcggcgac cccgggaaaa cgtccgcgtc
     3481 cccgtcgtcg attggcggga aagcgttttt ccccaatccc acccggattc gttataattt
     3541 ccgtcatggt ttctcccgcc gcgccccgtc ccggaacgcc cctgcggtgc agtcactgta
     3601 cccaacgagg gtcgggcgtg ttttgcgccc tgaacgccga cgccgtcaaa aagctggacg
     3661 gcgcccagac cgagaacctg tttaaaaaag gccaaatcct tttttacgag gggaacccgg
     3721 ccgtcggggt ctactgcgtc tccgccgggg aagtgaagct ttccaaaacc ggggccggcg
     3781 ggcggccgca aattctgggc atcgtgggcc ccggcacctt gatggggcac cgttccctct
     3841 tgaccaacca gccccacacc atgaccgccg aagccctggt ggactcccgg gtgtgctttt
     3901 tcgagaaaaa gtttttcctg gacctgctga accaagaacc gtcggtgtcc atcgaagtcc
     3961 taaaaaaact ttcccaggat ttggacgccg tggaagaccg cttgctggat ttggtggaaa
     4021 agccggttcc cgtgcggctg gcccggcttc tattgatgct caaggaggtc cacggccgcc
     4081 cgtcgcccaa gggcgtggtg ctcacgctgg gcctcacccg ggaggaaatg gccgaaatga
     4141 tcggcaccac ccaggaaacg accatccgcc tgctcagccg gttcaagcaa aaagggtacc
     4201 tccgactcga caaacggacc atcgttctcg ccgatattcc cggccttctc aaagtcgccc
     4261 gcctctccct gtaacgcttc cctgacgacc gtcagggctc ccgctgtccc ccgtcatttc
     4321 gccggccgtc cgttccgggt aggataaaac cagccgttca aagggaggtt ccgtggaccc
     4381 ttcgctcctc tcgaccggcg gggcccccgc gcccgacacg gcgccctggc acgcctggtc
     4441 cgttgaaaga acccgaaccg ccctgggcgc ccccgccgtc gggctttcgg aatcggacgc
     4501 cgcgggacgg ctggcccggg acggtcccaa ccggctttcg ccccccgtcc gccgatcccc
     4561 gtcccgactc tttcttgccc aattcaacga tttcatgatc cgggtccttc tgggggccgc
     4621 cctggtggcg ggcctcctgg gcgaccgggg ggacgccgcc gcgattttgg ccatcgtcct
     4681 cctgaacgcc ggggtcggtt ttgtgcagga gtttcgcgcg gagcgcgccc tggacgccct
     4741 gcgggaaatc gacgcgccca ccgcccgggt ggaacgggac ggccgccgcc ggttgatcaa
     4801 cgccgatggg ctcgttcggg gggaccgcgt gatcctcgaa gcgggggccc gcgttcccgc
     4861 ggatcttcgg ttgtcggagg cccaaaccct tcgaatcgac gaatccgctc tgacggggga
     4921 atccgccccc gtggaaaaaa acaccgaccc ccttccgata aaaaccgaaa tggccgatcg
     4981 gacgaacatg gcgtaccggg gaaccctcgt ggtgcacggc cggggagc
//
LOCUS       DAOQLS010000067         4937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7938_length_4937_cov_5.294756, whole genome shotgun sequence.
ACCESSION   DAOQLS010000067 DAOQLS010000000
VERSION     DAOQLS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7938_length_4937_cov_5.294756"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1183
                     /locus_tag="PK362_02015"
     CDS             263..1183
                     /locus_tag="PK362_02015"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNC73649.1"
                     /translation="MTAHARWMTGLMVATAVLAIVYFAPERGPVRLIAPTPPVPANPH
                     RITVIYMDEEIWLEREKGGWWIVRPHRWRAAGERVEKLLDTLGRFSLWNKITDKTDTD
                     SLYELSDRRVTVRVVGSNEQETLEWSFGKNTDVKNRLFARCSARSGVYAVDGLSRASL
                     GYTWGHWMDARLFPFEADDPVIEAAGKGPRGAFHLRKDSVGWTLDGRRADSAAVETWT
                     RALAEVRFLQWESPTPPVSGHGPALHLRSARGRHWSVEMDTSPESNGNFTLRHSDLPG
                     HLLLTGPNANVLFPTPAALQKHRGTLKPAD"
     gene            complement(1180..2322)
                     /gene="wecB"
                     /locus_tag="PK362_02020"
     CDS             complement(1180..2322)
                     /gene="wecB"
                     /locus_tag="PK362_02020"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNC73650.1"
                     /translation="MKRSTPKKILFVFGTRPEAIKMAPVILALRKRPAEFNVRIAVTA
                     QHRDLLDPVLKLFGIRPHHDLGIMRPGQSLTDVSVRALSRLEPVIRSEKPDLVLVHGD
                     TTTALMGALAAFYQRVPVGHVEAGLRSGDDANPFPEEANRRLTDGLSVLHFAPTVDAR
                     RNLLREGRPASGVVVTGNTAVDALDLGLARLGSQTPALPPGVARIARGPFVLATVHRR
                     ENFGRPLEEICEALGRVARARPDRGFVLPVHPNPNVGAVVRKILGSRSNVALTAPLEY
                     GPMIYLLKKSLFVVTDSGGIQEEAPSLGKPVLVVRRVTERPEAVRAGVARVIGTDGAS
                     VQRRVERLLDDGPLYRRMARRVHPFGDGRAAERIRRGLVRFFRSQR"
     gene            complement(2319..3314)
                     /locus_tag="PK362_02025"
     CDS             complement(2319..3314)
                     /locus_tag="PK362_02025"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNC73651.1"
                     /translation="MTPSFAALSRRWGVLDVPDERKVHRTPIPRWGGGAIAAGVFAAL
                     GALALFVPRFRTLLAYQHRVVKDGQIVGTLGLGSQLTGILLGASFCVVLGMWDDRRSL
                     PPIVKLLAQIIAAYVAMMYGVRMAGLALPGLGFVEFPLFLSQIITLLWLLGFMNAINL
                     ADGLDGLAAGLAAIAAVTFLFVALLQGETRIVLYAKQLKLAAVLAAAVGGACLGFLIF
                     NFHPARIFMGDGGTLFLGFMLGSISVVGTLKTSAVLAFLIPVIVVALPVTDTAFAIVR
                     RYRRGNGVMAADRGHFHHRLLDLGWTQREVVLLVYVLTLILSMAALLLTFFRGRV"
     gene            complement(3372..4625)
                     /gene="glyA"
                     /locus_tag="PK362_02030"
     CDS             complement(3372..4625)
                     /gene="glyA"
                     /locus_tag="PK362_02030"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNC73652.1"
                     /translation="MSELKKADPQVYRIVQDETHRQEEGLEMIASENYVSEAVLEAQG
                     SVLTNKYAEGYPGKRYYGGCEEVDKVETLAIERVKALFGAEHANVQPHSGSQANMAVY
                     MAALQPGDTIMGLELSHGGHLTHGHPLNFSGKYFKIVSYGVHPETERVDYDKLEALAR
                     EHKPKMIVSGASNYSRLWEWERLRAIADSVGALHFADIAHYAGLIAAGEYPSPVAHAD
                     FVTTTTHKTLRGPRGGVVMCKERFAKDIDRVVFPGVQGGPLMHVIAAKAVAFGEALKP
                     AFKKYQAQVRVNAQALAAALVKHGFRIVAGGTDSHVMSVDLRPKSVTGKDAEVVLGKA
                     GITVNKNTIPFDPEKPFITSGVRLGTPALTTRGMKAKEMEKIAGWINAAIEGRSDDKA
                     LAKIRREVETLCKKFPLYRHRLKRG"
     gene            complement(4636..>4937)
                     /locus_tag="PK362_02035"
     CDS             complement(4636..>4937)
                     /locus_tag="PK362_02035"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNC73653.1"
                     /translation="TTGGKDTLALRVPDHPVARALLRAMNAPLAVTSANRSGRPEATT
                     GARAAALFGGKVDVLVDGGPCPGGVPSTVLDVSSVVWTLRREGAVPKSDLLRYMA"
BASE COUNT          925 a         1587 c         1608 g          817 t
ORIGIN      
        1 gcttgtcgtt atcggatttt accggcggcg ccgggaccgg tggcgggcga tctggcgcgc
       61 ggaggtgggt cgtgtttaag ccgagggaga ataattcctt aacggccggg cgaccggaaa
      121 tcgatcggac tctcaccggg ggccgaacgg ccgagaaagg cgtcgaaacc tcgccgtcgc
      181 gaaaaatccg tttaagcggg gacccgcttt ttcccgcttc ggagggaggc gcccagtcgc
      241 cgggcgttaa accgccgaaa caatgaccgc ccacgcccga tggatgacgg gattgatggt
      301 cgcgacggcg gttttggcca tcgtttactt cgccccggaa cggggaccgg ttcgtctcat
      361 cgcccccacc ccccccgtcc cggccaatcc ccatcgaatc acggtgattt acatggacga
      421 agaaatttgg ctggaacgtg aaaagggggg ctggtggatt gttcgtcccc accggtggcg
      481 cgccgccggg gaacgcgttg aaaagctttt ggacaccctc ggccgattct ccctttggaa
      541 caaaatcacc gacaaaaccg acaccgattc cctttacgaa ttaagcgatc gtcgcgtgac
      601 cgttcgggtc gttggaagca acgaacaaga gacgcttgaa tggtcttttg ggaaaaacac
      661 ggacgtgaaa aaccgacttt tcgcgcgttg cagcgcacgg tcgggggtgt atgcggtgga
      721 cggtttatcc cgggcgagct tggggtatac ctggggccat tggatggatg cgcggttgtt
      781 tcccttcgaa gcggacgacc ccgtcattga ggcggcggga aaggggccgc gcggggcttt
      841 tcacctccga aaggattcgg tgggatggac cctcgatggt cgccgggccg actccgccgc
      901 ggtggaaacc tggacgcggg ccctggccga agttcgattc ttgcaatggg aaagcccaac
      961 gccgcccgtg tcggggcacg ggcccgccct ccacctccgc tcggcccgag gccgacattg
     1021 gtcggtcgag atggacactt caccggagag caacgggaat tttaccctcc gccactcgga
     1081 tttgccgggg catctccttc tgaccggtcc aaacgccaac gtccttttcc ccactcccgc
     1141 ggcgctccaa aaacaccggg gaaccttgaa gcccgccgac tagcgctgcg accggaaaaa
     1201 ccggacgagc ccgcgacgga tgcgctccgc ggcccggccg tccccgaaag ggtgtacccg
     1261 tcgggccatg cgtcgataga gcggcccgtc gtccaatagc cgttccaccc gtctctgcac
     1321 cgacgcgccg tcggtgccga tcacccgggc gaccccggcg cggacggcct cgggccgctc
     1381 cgttacccgg cgcaccacca acacgggctt gcccaggctc ggcgcttcct cttgaatccc
     1441 cccggaatcc gtcacgacga aaagggattt ttttagcagg taaatcatcg ggccgtattc
     1501 caagggcgcc gtcaacgcca cgttggaccg ggacccgagg atttttcgca ccacggcgcc
     1561 cacgttgggg ttgggatgga cggggagcac gaacccccga tccggccggg cccgggccac
     1621 gcgccccagg gcttcgcata tttcctccag gggccgtccg aaattctccc gccggtgcac
     1681 ggtcgccaaa acaaaaggac cccgggcgat ccgggccacc ccgggcggca gggccggcgt
     1741 ttgagatccc agacgggcca atcccaaatc cagggcgtcg accgccgtgt tccccgtgac
     1801 gaccacgccg gaggccgggc gcccctcccg caacaaattc cgccgggcat caacggtggg
     1861 ggcaaaatgg aggacgctca ggccgtcggt caaccgtcta ttcgcttcct ccggaaaggg
     1921 gttggcatcg tcgccggagc gaaggcccgc ctccacgtgt cccacgggaa cccgctgata
     1981 aaacgcggcc agggccccca tcagggcggt ggtcgtgtcg ccgtggacca acacgaggtc
     2041 cggcttttcg gagcgaatca cgggctccag gcgggacaac gcacggaccg aaacgtccgt
     2101 caacgactgg cccgggcgca tgattcccaa atcgtggtgg ggacgaattc caaagagctt
     2161 caaaacgggg tccagcagat cccggtgctg ggcggtcacg gcgattcgca cgttgaattc
     2221 cgccggacgc ttccgaaggg ccaaaatgac cggggccatt ttgatggcct cgggccgggt
     2281 gccgaacaca aaaaggattt tcttgggggt cgaacgtttc acacgcggcc ccgaaaaaac
     2341 gtcaacagga gcgccgccat ggacaagatc aacgtgagca cgtaaaccaa caagacgacc
     2401 tcccgctggg tccagccgag gtccaggagt cggtggtgga agtgcccccg gtccgcggcc
     2461 atcacgccgt ttccccgacg gtaacggcgc acgatggcaa acgccgtgtc ggtcaccggc
     2521 agggcgacca cgatcaccgg gatcaggaag gccagcacgg cgctggtctt taacgtgccc
     2581 accacgctga tggacccgag catgaagccc aaaaagagcg tgccgccgtc ccccatgaaa
     2641 atgcgcgccg ggtgaaaatt gaatatcaaa aaacccaggc aggccccgcc gacggccgcc
     2701 gccaggaccg ccgccaattt gagttgcttg gcgtaaagga cgatccgggt ttccccctgg
     2761 agcaacgcca cgaacaagaa ggtcacggcg gcgatggccg ccaaccccgc ggccagcccg
     2821 tcgagcccgt cggccaagtt aatggcgttc atgaatccca acagccaaag gagcgtgatg
     2881 atttgggata ggaacagggg aaactcgacg aaccccaggc cgggcagggc caagccggcc
     2941 atgcgaacgc cgtacatcat ggccacgtaa gcggcgatga tttgggcgag cagtttgacg
     3001 atgggaggga gggaacggcg gtcgtcccac atgccgagga caacgcaaaa ggacgcgccc
     3061 aaaaggatgc ccgtgagttg ggaaccaagc cccagagtgc ccacgatctg cccgtccttg
     3121 accactcggt gttggtaggc caacagcgtg cgaaatcggg gaacgaacag ggccaaggcg
     3181 cccagggccg cgaagacccc ggcggcgatg gcgccgccgc cccagcgggg gatcggcgtc
     3241 cggtgaactt ttcgctcgtc gggaacgtcc aggacccccc agcgccgcga gagagcggcg
     3301 aaggagggcg tcaagcccgc ggcgaccgcg agggccgtga aaaaaccgac ggcgagcagg
     3361 aggggcagga attatccccg tttcaatcgg tggcggtaga ggggaaactt tttgcagagc
     3421 gtttccactt cccgccggat tttggccagg gccttgtcgt ccgaacggcc ctcgatggcc
     3481 gcgttgatcc agccggcgat cttttccatc tccttggctt tcatgccccg ggtggtgagg
     3541 gccggggtgc cgaggcggac cccggaggtg atgaagggct tctccggatc gaaggggatc
     3601 gtgtttttgt tgacggtgat gccggccttc cccagcacga cttcggcgtc tttcccggtg
     3661 acggatttgg gccggagatc gacggacatg acgtggctgt cggtcccccc cgccacgatt
     3721 cgaaaaccgt gcttgaccag ggccgcggcc agggcttggg cgttgacgcg cacctgggcc
     3781 tggtactttt tgaaggcggg tttcaacgcc tcgccgaagg cgaccgcttt ggccgcgatg
     3841 acgtgcatca acgggccgcc ctgaaccccg ggaaacacca cccggtcgat gtccttggcg
     3901 aaccgctcct tgcacatcac gacgccgccc cgggggcccc gaagggtctt gtgggtggtc
     3961 gtggtgacga aatcggcgtg ggccacgggc gaggggtatt ccccggcggc gatcaagccg
     4021 gcgtaatggg cgatgtcggc gaagtgcagg gccccgacgg aatcggcgat ggcccgcagg
     4081 cgttcccact cccagaggcg ggaataattg gacgcgcccg agacgatcat tttgggcttg
     4141 tgttcccggg ccagggcctc taacttgtcg taatccacgc gctcggtttc ggggtgaacc
     4201 ccgtaggaca cgatcttgaa atacttgccg gaaaagttga gcggatggcc gtgggtcaga
     4261 tgcccgccgt gggacaattc caaccccatg atcgtgtccc cgggctggag ggccgccatg
     4321 tagacggcca tgttggcctg ggacccggag tggggctgca cgttcgcgtg ctcggcgccg
     4381 aacagggcct tgacccgctc aatggccagg gtctccacct tgtccacttc ctcgcagccg
     4441 ccgtagtagc gcttccccgg gtagccttcg gcgtatttgt tcgtcagcac cgatccctgg
     4501 gcttccagga ccgcttcgga cacgtagttc tccgaagcga tcatctccaa accctcctcc
     4561 tgccggtggg tttcgtcctg gacgatccga taaacttggg ggtccgcttt cttcaactcg
     4621 ctcatcgctg tctccttagg ccatgtatcg aagcaaatcc gatttgggca cggcgccttc
     4681 ccggcgcagg gtccacacca cgctggacac gtccagcacc gtcgacggaa cgccgccggg
     4741 gcaggggccg ccgtccacca acacgtccac tttcccgccg aagagggccg ccgcccgggc
     4801 gccggtcgtg gcttcggggc ggccggaccg gttggcgctc gtgaccgcca ggggcgcgtt
     4861 catggcccgc aacaaggccc gggccacggg gtggtcggga acgcgcaggg ccagggtgtc
     4921 ctttcccccc gtggtcc
//
LOCUS       DAOQLS010000068         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_7985_length_4920_cov_3.694553, whole genome shotgun sequence.
ACCESSION   DAOQLS010000068 DAOQLS010000000
VERSION     DAOQLS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7985_length_4920_cov_3.694553"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PK362_02040"
     CDS             complement(<1..670)
                     /locus_tag="PK362_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNC73654.1"
                     /translation="MLKINNMSVGKKIGSLSALMIGGIVLSAGTGLKVMRSIHAGLDN
                     IANVQLPATRKGASAEIGFRVLYSCVYRALNVTRETPPAVINEINADLELQGETFRSA
                     LLDLDGLALNKPTKDALGLVQMASETYLADCEEVVRLATHANGAAARARMKDLNSQFR
                     QMEDSLYVLSEEILKESTHRTAQAQRWHRWGTGVLSGVGLAVVAIAIFLAVWIIRGIT
                     RPLAE"
     gene            complement(725..1783)
                     /locus_tag="PK362_02045"
     CDS             complement(725..1783)
                     /locus_tag="PK362_02045"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNC73655.1"
                     /translation="MTAAAPLHVLVVDDSAVVREVLTDLLSQDPGLTVATAGDPMIAM
                     TKMRIQRPQVIVLDLELPRMDGLTFLRKIMTEDPIPVVVCSSQTPAGSDEALRALEEG
                     AVDVIAKARLGVREFLRESSDRLLDAVRSAATARVAAARRPPAVPFLGSKPLLTVTTD
                     KVMALGASTGGTEALREILQALPPSAPGVVIVQHMPEGFTNAFARRLDSICRVEVKEA
                     ADGDRVLAGRALIGPGNRHTTLRRSGGHYIVEVKDGPLVSRHRPSVDVLFESVAAEAG
                     RNAVGAILTGMGSDGARGLLAMRNNGARTLAQDEASCVVFGMPAVAIAQGAVDRVVPL
                     ERMAEVLLAEANALSGKA"
     gene            complement(1780..2763)
                     /locus_tag="PK362_02050"
     CDS             complement(1780..2763)
                     /locus_tag="PK362_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNC73656.1"
                     /translation="MTEPRPRKTDPTGTSFPWGPLPPDLRRLDREMAQGLGFPSLTDA
                     EFSRLRDWVEKESGIHLTDAKRGLVMGRLAKRVRAVGATGFGAYIERALGSAGPEERA
                     RLLDALCTNETHFFREIHHFDFLEKRVFPSWRVTGTTGRIPRHIRAWSAACSTGEEPY
                     ILAMVLLKNFPRSSGWSVEVLGTDLSGHALQRAAAAVWPIEKAAEIPSAFLKAFMRRG
                     TGPEEGKMKAGPEIREAVHFLRLNLCAEAYPVSGAFDLIFCRNVLIYFNWDTKRKVIG
                     RLLSHLAPTGYFFSGHAESLNELSNVVTTLQPTVYRPLRTADSTDPTREAP"
     gene            complement(2760..4325)
                     /locus_tag="PK362_02055"
     CDS             complement(2760..4325)
                     /locus_tag="PK362_02055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNC73657.1"
                     /translation="MAIDRRALLATFRQQAGETLRGLEESLLALEAHPDDEELVNGLF
                     RGAHRLKGDAALLGLAPIAELTHVFEDLLDRLRHRKWSVTGSMITLHLRFLDVLEELI
                     RGVGGPSVAEAASASGPAGGGVPTAKTVRVNTDKLDRLLDTVGEIAIHREKLRQMVED
                     GSSGGGAALAETLREADPLYLDLQETVMKARMVPIGPLFRTFQRTVRDLGNALGKKAV
                     LRLTGEEVEVDTAIVENLRDPLTHLIRNAMDHGVEAAEARAAAGKSAEGQLSLSAAQE
                     AGFVHVRLTDDGRGLDRAAIAARVRALGLAAAPEALTDADLFAFLFAPGFSTATKVTE
                     WSGRGVGLDIVRRHVQTLRGIIAVESRPGQSTTFAIRLPLTLAIIQGFSVGVGEETMV
                     IPLEMVAECLELAPGERENRDPLGVFNLRGEPVPLVRLGALWGGAPGPAPARESAVVV
                     RWGDRRAGFVVDRVLGENQTVIKPLPPHFRSLEGVAASSLLGNGRIALVLDVPGIFKK
                     LLRERAVSGRERP"
     gene            complement(4335..>4920)
                     /locus_tag="PK362_02060"
     CDS             complement(4335..>4920)
                     /locus_tag="PK362_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNC73658.1"
                     /translation="FWGGRAPTFMGNDPRLPSPSASPARADFWARDRAASGVPTPVFR
                     GRPVVYVAPGELMATTLARAARTLLGSCVAVCLWDGNLRIGGMTHYLLPDSNGHPDVT
                     PRYGDVAVAVLLERLHRLGARVERLEAKIFGGARVLPVFPGDGARVGDKNIALARDIV
                     GRARIPIRGEDVGGDRGRKLIFCTDDGSAWVRRL"
BASE COUNT          897 a         1717 c         1527 g          779 t
ORIGIN      
        1 cctcggccag gggccgcgtg atgccgcgaa tgatccacac cgcgagaaaa atcgcgatgg
       61 ccaccacggc caaccccacc ccgctcaaaa cgccggtgcc ccatcgatgc catcgctggg
      121 cttgagcggt tcgatgggtc gactccttca ggatctcttc cgacaacacg taaaggctgt
      181 cttccatttg ccggaattga ctgttcaaat ccttcattcg cgcgcgcgcc gccgccccgt
      241 tcgcatgggt ggccaggcga acgacttctt cgcaatcggc caggtaggtt tccgacgcca
      301 tctgcaccag ccccagggcg tccttcgttg gcttattcaa cgccagtccg tccaggtcca
      361 acagagcgga ccggaacgtt tcgccctgga gctccaaatc ggcgttgatc tcgttgatca
      421 cggcgggcgg cgtttcccgg gtcacgttga gcgcgcggta gacgcaggaa tagagcaccc
      481 gaaatccgat ttcggccgag gcgcccttgc gggtcgcggg caattggacg ttggcgatgt
      541 tgtccaggcc cgcgtgaatg gaccgcatga ccttcaagcc cgttcccgcg ctgagcacaa
      601 tgcccccgat catcaaggcc gaaaggcttc ctattttttt tcccaccgac atattgttaa
      661 tcttcagcat ttcccgcctc ctcgaaaaat ccccgggggg agccgccgct cccgaaagac
      721 attctcacgc cttgccggaa agggcgttgg cttcggccag aagaacctcg gccatccgct
      781 ccaggggcac cacgcggtcc acggccccct gggcgatggc cacggccggc ataccgaaca
      841 ccacgcagga cgcctcgtcc tgggccaacg tccgcgcccc gttgttccgc atcgccaaaa
      901 gtccccgggc cccgtcggac cccatgccgg tcaaaatcgc ccccaccgcg tttcgcccgg
      961 cctcggcggc cacggactcg aacaggacgt cgacgcttgg gcggtggcgg gagaccaagg
     1021 gtccgtcttt gacctccacg atgtaatgcc cgccgctccg gcggagggtc gtgtgccggt
     1081 tccccggccc gatcaacgcc cgacccgcca gcacccggtc gccgtccgcc gcctccttga
     1141 cttcgacgcg gcaaatcgaa tccaaccgac gggcgaaggc gttggtgaag ccttcgggca
     1201 tgtgttggac gatcacgacc cccggcgcgg agggaggaag ggcttgaaga atttcccgaa
     1261 gggcctccgt tccgcccgtg gaggctccca acgccatgac cttgtcggtc gtgacggtca
     1321 aaagcggctt ggacccaagg aaggggaccg ccggcgggcg gcgcgccgcc gcgacccggg
     1381 ccgtggcggc gcttcgaacc gcgtccaaca accggtccga ggattcccga agaaattcgc
     1441 gcacgccgag cctggccttg gcgatgacgt cgaccgcccc ctcctccagg gcccgcaagg
     1501 cctcgtcgga acccgccggg gtttgactgg agcaaaccac cacggggatc gggtcttcgg
     1561 tcatgatttt ccggaggaag gtcagcccgt ccatgcgggg cagctccaaa tccaacacga
     1621 tgacctgcgg tcgttgaatc cgcattttgg tcatggcgat catcggatcg ccggcggtgg
     1681 ccacggtcag gccggggtct tgggacaaga ggtccgtgag gacctcccgc acgacggcgg
     1741 aatcgtcgac gaccagcacg tgcaacggcg cggcggccgt cacggcgcct cccgcgtcgg
     1801 atcggtcgag tcggccgtcc gcaacggacg gtagaccgtg ggttggaggg tggtcacgac
     1861 gttcgatagt tcgttcagac tttccgcgtg accggagaaa aagtaaccgg tcggggccag
     1921 gtgggaaaga aggcgaccga tgactttccg cttggtgtcc caattgaagt aaatgaggac
     1981 gtttcggcaa aaaatcaaat cgaaggcgcc ggacacgggg taggcctcgg cgcagagatt
     2041 caggcgaagg aaatgcaccg cttcccgaat ttccggcccg gccttcattt tcccctcctc
     2101 gggtcccgtg ccccgccgca tgaacgcttt gagaaacgcg ctggggatct ccgcggcttt
     2161 ttcaatgggc cacaccgccg ccgcggcccg ttgaagggcg tggccggaga ggtccgtgcc
     2221 cagcacctcg acgctccaac cgcttgaccg ggggaaattc ttgaggagga ccatggccaa
     2281 gatgtacggc tcctcccccg tcgagcaggc cgcgctccac gcccggatgt ggcggggaat
     2341 gcggcccgtg gttcccgtga cgcgccacga cgggaacacc cgcttttcca aaaaatcaaa
     2401 atggtggatt tcccggaaga aatgcgtttc gttggtgcaa agggcgtcca acagccgcgc
     2461 ccgttcctcc gggcccgcgc tccccaacgc gcgctcgata tacgccccga agccggtcgc
     2521 tcccaccgcg cggacccgct tggccaaccg gcccatcacc aagccgcgct tggcgtcggt
     2581 caagtggata ccggattcct tttcgaccca atcccgaagc cgggagaact ccgcgtccgt
     2641 caggcttgga aagcccaacc cctgggccat ttcccggtcc agccggcgga gatcgggggg
     2701 gagcggcccc cacggaaagg acgtcccggt cgggtccgtt ttccggggac ggggctcggt
     2761 cacggccgtt ccctgccgga caccgcccgc tcccggagca gttttttgaa aatgccgggg
     2821 acgtccagca cgagggcgat ccgcccgttc cccaacaaag acgacgccgc caccccctcg
     2881 agcgatcgaa aatgaggcgg aaggggcttg atcaccgttt gattctcccc caacacccgg
     2941 tccacgacga accccgcccg gcgatcgccc caacgcacga cgaccgcgct ctcccgggcc
     3001 ggggccggac ccggcgcccc cccccacaag gcccccaacc ggacgagggg gacgggctcc
     3061 ccccggagat taaacacgcc caagggatcc cgattctccc gctccccggg ggccaactcc
     3121 agacactcgg ccaccatttc cagggggatc accatcgttt cctcgccgac cccgacggaa
     3181 aaaccttgaa tgatcgccaa cgtgaggggc agccgaatgg cgaacgttgt ggattggccg
     3241 ggacgcgatt ccacggcgat gattccccgc agcgtctgaa cgtggcggcg gacaatgtcc
     3301 aaaccgacgc cccgcccgga ccactccgtc acttttgtcg cggtggaaaa acccggcgcg
     3361 aaaagaaacg cgaacaagtc ggcgtcggtc agcgcctcgg gggccgccgc cagccccaag
     3421 gcccgaaccc gcgccgcgat cgccgcgcgg tccagcccgc gcccgtcgtc ggtcaaccgc
     3481 acgtggacga accccgcctc ctgggccgcc gaaagggaca attggccctc cgcggacttg
     3541 ccggcggccg cgcgcgcttc cgcggcttcc acgccatggt ccatggcgtt tcgaatcaaa
     3601 tgcgtgagag ggtcccgaag gttttccacg atggccgtgt ccacctcgac ctcctccccg
     3661 gtcagccgca ggaccgcttt tttccccaac gcgttgccca aatcccggac cgtgcgctgg
     3721 aaggttctga aaagcggtcc aatcgggacc atgcgggctt tcatcaccgt ttcctgaaga
     3781 tccaaataga gcggatcggc ttcccgaagg gtctcggcca aggccgctcc cccgccggag
     3841 gagccgtcct ccaccatttg gcgcagtttt tcccgatgga tggcgatttc gccgaccgtg
     3901 tccaggaggc ggtcgagttt gtccgtgttc acgcgtacgg ttttggccgt cgggacgccg
     3961 ccgccggcgg gacccgacgc cgaagcggct tccgcgaccg acgggccgcc caccccccga
     4021 atcaattcct ccaggacatc gagaaaccga aggtggagcg tgatcatcga tcccgtgacc
     4081 gaccatttcc gatggcgcaa tcggtccaga aggtcttcga acacgtgggt caattcggcg
     4141 atgggggcca agcccaggag ggcggcgtcg cctttcaatc gatgggcgcc gcgaaaaagg
     4201 ccgttgacca attcctcgtc gtcgggatgc gcttccaagg ccaacaagga ttcctccagc
     4261 ccccggagag tttccccggc ttgctggcgg aaagtcgcca gaagggcccg acggtcaatg
     4321 gccacggcgg cgcctcacag tcggcgaacc caggcggagc cgtcgtcggt gcaaaagatc
     4381 aatttccgtc cgcggtctcc ccccacgtcc tccccgcgga tcggaatgcg ggctcgcccg
     4441 acaatgtccc gggcgagggc gatgtttttg tcccccaccc gggcgccgtc gccgggaaac
     4501 accggaagga cccgggcccc accgaaaatc ttggcctcca accgctccac gcgggccccg
     4561 aggcggtgaa gccgctcgag aaggacggcg acggccacgt ccccgtaccg aggcgtcacg
     4621 tccgggtgcc cgttggaatc gggcagcaag taatgggtca ttccaccaat gcgtaaattc
     4681 ccgtcccaga ggcagacggc cacgcaggac cccaggaggg tgcgggccgc ccgggccagc
     4741 gtggtcgcca tcaattcccc gggagccacg taaacaacgg gccgcccgcg aaagacgggc
     4801 gtgggaacgc cggaagcggc ccggtcgcgg gcccagaaat ccgcgcgggc cggcgaggcg
     4861 gagggcgacg gaagccttgg gtcgtttccc atgaacgtcg gggccctacc cccccaaaat
//
LOCUS       DAOQLS010000069         4872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8124_length_4872_cov_3.767698, whole genome shotgun sequence.
ACCESSION   DAOQLS010000069 DAOQLS010000000
VERSION     DAOQLS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8124_length_4872_cov_3.767698"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PK362_02065"
     CDS             complement(<1..180)
                     /locus_tag="PK362_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC73659.1"
                     /translation="MHKLLARQWKRHVGGPPEDRPDLAAFLSSVNVAYEQFDRDRLLL
                     ERSIDISSKELLEANR"
     gene            complement(185..1327)
                     /locus_tag="PK362_02070"
     CDS             complement(185..1327)
                     /locus_tag="PK362_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNC73660.1"
                     /translation="MRVERRQWKSGLGWSAGSTEPLAGDAQVVFVFGAGAHLKDPARI
                     KDIREMYPRALRVGCSTAGEIFDVNVSDETLVASALALDKGRVVPVLVPVPSPAESRR
                     AGEALAAGLPPEGLVHVVVLSDGLHVNGSDLVRGLTSGLPRGVRVTGGLSADGDRFVE
                     TGVLLGDDFHSRAVVGIGFYGERLRVGFGSLGGWDTFGPRRLITKSNGNVLECLDGKS
                     ALEIYKNYLGEYASGLPATGLLFPLAIQRPQDPEVLVRSVLAVDEIKGTMTFAGDMPE
                     GCLAQFMMANFDRMVDGAESAAGHAKIGFGASDAEWALLISCVGRKLILKQRVEEEVE
                     AVRDVLGPRPVLVGFYSYGEIAPFRPDARCDLHNQTMTITTFTEIP"
     gene            complement(1317..2933)
                     /locus_tag="PK362_02075"
     CDS             complement(1317..2933)
                     /locus_tag="PK362_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC73661.1"
                     /translation="MDDEPGFRALLSMELTKRGHRVVTAENGRDALEQCRRNEFQLVI
                     SDVKMPKMGGLEFLEALKKDSPSTEFIITTGYGTVETAVAAMKKGAYDFIQKPYNLPE
                     LLALVEKALEKGELRAMVGVYEASREVFKSVKLDTLLPAIMEWTVKILGADDASLMLV
                     DDTGKLRLEASTGLTADQRRGVEIRLGEGVDGRAAQWREPVLISGPLWQDPRFADLRV
                     VRDIKSSIVYPLVLEGELLGVLNANRTKREDPFSGSSLFSMTILGSQIAQAVYNARLY
                     KKLEGHVEELKTAYADLRNTQAQLIQSEKMAALGVLSAGVGHELNNPLSAVIGMAQFL
                     LEGGGLSAEQREDIEMILRQSQRCATIVQSLLDFSRKSEPRKIPTDLAGVVDCVLRLV
                     RGDFLKEGLTIDAAYEPETPRVNADPDQLHQVFLNAATNARQAMEGRSGGVLTVTVGR
                     DGNKACVVFRDTGAGIPPDIQAKIFDPFFTTKKPGQGTGLGLSISLGIVKEHQGEIRV
                     AGRPGAGAEFRFLFPPLEEQTKAPEALRAS"
     gene            3274..>4872
                     /locus_tag="PK362_02080"
     CDS             3274..>4872
                     /locus_tag="PK362_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC73662.1"
                     /translation="MVERLKEWVFGRPKNPLDPHVFHNISLIAFFAWVGLGADGLSSS
                     CYGPEEAFRALGHYTHLAIPLALAVAATVFILSASYSQIIEYFPTGGGGYLVASKLIG
                     PVSGLISGCALLVDYILTVAISIASSMDAIFSFLPHHLHHYKFAATLVFLLILVTLNL
                     RGIKESVQILTPIFVTFVITHTIVIVYAIFSHGTQLPALVGDTLAETRQGIQSVGLLA
                     MSGIFLRAFCLGGGTFTGIEAVSNGLQILREPRVKTGKRTMKYMAFSLAFTAGGILIS
                     YVLNGVSHTPGQTLNAVLIGGLTEKWPLGHAFLLVTLLSEGALLIVAAQAGFLDGPRV
                     MSNMAIDSWLPRRFTNLSDRLVIKDGVLVIGLSALAMLIYTKASVGLLVVMYSINVFL
                     TFSLSQAGMVRHWVRDRGDGWPRKLAINVTGLILTLGILIVTVFIKFREGGWITLVIT
                     GVIVAFCLWVNHHYRDTAKALHHLDEILTQIPLPEEMPVVKKVPGKPAAILMVNGYNG
                     MGIHSFLAIHRLFPGHFKNFVFLAV"
BASE COUNT          908 a         1567 c         1397 g         1000 t
ORIGIN      
        1 ccggttggcc tccaacaact ccttggaact gatgtcgatg gaccgttcca acaggaggcg
       61 gtcccggtcg aactgttcgt aggccacgtt caccgaggac aagaaggccg ccaggtccgg
      121 ccggtcctcc ggcgggcccc ccacgtgccg cttccattgg cgggcgagca atttgtgcac
      181 ggcgctaagg gatttcggtg aaggtcgtga tcgtcatggt ttggttgtgc aggtcgcagc
      241 gggcgtcggg acgaaagggc gcgatttccc cgtaggaata aaagcccacc aggaccggac
      301 gggggccaag gacgtcccgg acggcctcca cctcctcctc cacccgctgc ttgagaatta
      361 attttcgtcc cacgcagctg atgagaagag cccattcggc gtccgacgcg ccgaacccga
      421 ttttggcgtg ccccgccgcc gattcggcgc cgtcgaccat ccggtcaaaa ttcgccatca
      481 tgaattgggc caggcacccc tcgggcatgt cccccgcgaa ggtcatggtg ccttttattt
      541 catccacggc cagcaccgac cggaccagga cctccggatc ctggggccgt tggatggcca
      601 agggaaacaa caagcccgtg gccggaaggc cgctggcgta ttcgccgaga taatttttgt
      661 agatctccag ggccgatttg ccgtccaggc attccagaac gttcccgttg gatttggtga
      721 tgagccgacg ggggccgaag gtgtcccaac cgcccaggga tccgaaaccg acccgcagac
      781 gctccccgta aaaaccgatg cccacgacgg cccgggaatg gaaatcgtcg cccaggagga
      841 cccccgtttc aacgaagcgg tctccgtcgg ccgaaagacc gccggtgaca cggacccccc
      901 ggggcaatcc cgaagtcaat ccccgcacca aatcgctccc gttcacgtgg agtccgtcgg
      961 aaaggaccac gacatggacc aacccctcgg gcggcaggcc ggcggccaag gcctccccgg
     1021 cccggcggct ttcggccggc gaggggacgg ggaccaggac ggggaccacc cgccccttat
     1081 ccagggcgag ggccgacgcc acgagggtct cgtccgacac gttgacgtcg aaaatctccc
     1141 ccgccgtgga acagcccacc cgcagggccc ggggatacat ctctcgaatg tccttgatcc
     1201 gcgcagggtc ctttaggtgg gcaccggccc caaagacaaa aacgacctgg gcgtcccccg
     1261 ccaatggttc ggtggagccc gcggaccagc ccaaaccgga tttccactgc cgacgttcaa
     1321 ctcgcacgta gggcctccgg ggccttggtt tgttcttcca gcgggggaaa gaggaatcga
     1381 aattccgccc cggcccccgg gcggccggcg acccgtattt ccccttggtg ctccttcacg
     1441 atgcccaggg aaatggaaag ccccaacccg gttccctgtc ccggcttctt cgtggtgaag
     1501 aaggggtcga agatcttggc ctgaatgtcc ggcggaatgc cggcccccgt gtcccgaaaa
     1561 accacgcacg ccttgttccc gtcccgtccg acggtcaccg tgagcacgcc gccggaccgg
     1621 ccctccatgg cctggcgcgc gttggtggcc gcgttcagga acacctggtg caactggtcg
     1681 ggatcggcgt tgacccgggg agtttcgggc tcgtaggcgg cgtcgatggt caggccttcc
     1741 ttcaaaaaat cgccccgaac cagacgcagg acgcagtcga cgacgcccgc caaatccgtg
     1801 gggatcttgc ggggctcgga cttccggctg aagtccagga gggactggac gatggtcgcg
     1861 caccgctggc tttgacgaag gatcatttca atgtcctccc gctgttccgc actcagtcca
     1921 cccccttcca ataaaaattg agccatgccg atgacggcgg acagggggtt gttgagctcg
     1981 tgacccaccc ccgccgacaa cacccccaag gcggccattt tttccgattg aatgagctgg
     2041 gcctgggtgt tgcgcaaatc cgcgtaggcc gtttttaatt cctccacgtg accttccagc
     2101 tttttgtaga gacgggcgtt gtaaaccgcc tgggcgatct gggaacccaa aatggtcatg
     2161 ctgaaaaggg aggacccgga aaaggggtcc tcccttttgg tccggttcgc gttaagtacg
     2221 ccgagcaatt ctccttccag aacaagcggg tagacgatgg aggatttgat gtctcgaacc
     2281 acccgaaggt ccgcgaaccg agggtcctgc cagaggggcc cggaaatcag gaccggctcg
     2341 cgccattggg cggcccgacc gtcaacgcct tctcccagcc gaatttcaac cccccggcgc
     2401 tgatccgcgg tcaaccccgt ggacgcctcc aaacgcaact tccccgtgtc gtccaccaac
     2461 atcaaggagg catcatcggc gcccaatatt ttgaccgtcc actccatgat ggcgggcagt
     2521 agcgtgtcca gcttgacgga tttgaagacc tcccggctgg cctcgtacac ccccaccatg
     2581 gcccgaagct cccccttttc cagggctttt tccaccaggg cgagcagttc gggaaggttg
     2641 tagggttttt ggataaagtc gtaggcgccc tttttcatgg cggccacggc cgtttcaacg
     2701 gtcccgtaac ccgtggtgat gatgaattcg gtcgaggggg agtctttttt gagggcttcg
     2761 aggaattcca ggccgcccat tttcggcatt ttaacgtccg atataaccaa ttggaattcg
     2821 ttccggcggc actgttccag ggcgtcccgt ccgttttcgg ccgtgaccac ccgatgcccc
     2881 cgctttgtca attccatgga caaaagggcc cggaacccgg gctcgtcgtc caccaccaga
     2941 atatgatgcg gcagggtccc gccaaaagat tttggggccc cgaatccaga tgggctcatt
     3001 tggctccctc gctcgatcga tccatcctct ggattatcgt ccacgaacca ccgaaataga
     3061 gagaaaataa ggggcgcgat cccgggcgaa aggcggtcat tcgccggggt cttgacaggg
     3121 ggggtggatt tagtaagatt cagttcgccc ccaaggtcgg gggagaccat cgctgacgga
     3181 cattacaggg ctgtaattcg gaccgtctcg ttcccttaag gcttttttta atggagcgag
     3241 gcgctttttt ttagaccatc ggagttgctg aaaatggttg aaagacttaa ggaatgggtc
     3301 tttgggcgac ctaaaaatcc cctggacccc cacgttttcc ataatatttc cctcatcgcc
     3361 ttttttgcct gggtcggctt gggggcggac gggctgtcct cctcctgtta cgggcccgaa
     3421 gaagccttcc gcgccctggg ccattacacc cacctggcca tccccctggc cctggcggtc
     3481 gccgccacgg tctttatcct ttcggccagt tactcccaaa tcattgaata tttccccacc
     3541 gggggcggcg gctacctggt ggcctccaaa ctcatcggcc cggtgtcggg gctcatctcc
     3601 gggtgcgctc tcctggtcga ttacatcctg acggtggcca tttccatcgc ttccagcatg
     3661 gacgccattt tcagcttcct cccccaccac ctccaccact acaaattcgc cgccaccctt
     3721 gttttcctcc ttattttggt caccttgaat cttcggggga tcaaggagtc ggttcaaata
     3781 ttgaccccca tttttgtcac tttcgtcatc acccacacca tcgtcatcgt atacgccatc
     3841 ttttcccacg gcacccagtt gccggccctg gtgggagaca ccctggcgga aacccggcaa
     3901 ggcatccaat ccgtgggtct attggccatg tcgggaatat tccttcgggc cttctgcctg
     3961 gggggcggaa cgtttacggg gattgaggct gtttccaacg gtcttcaaat ccttcgggaa
     4021 ccccgggtga aaacgggcaa acgaaccatg aaatacatgg ccttctccct ggcttttacg
     4081 gccgggggca tcctgatcag ttacgttctg aacggcgtgt cccacacccc cggccaaacg
     4141 ctgaacgcgg tcctcatcgg agggctcacc gaaaaatggc ccctgggtca cgccttcttg
     4201 ctggtgacgc ttttgtccga aggcgccctt ttgatcgtgg cggcccaggc gggattcttg
     4261 gacggccctc gggtgatgtc caacatggcc atcgacagct ggctcccccg gcggttcacc
     4321 aacctgtcgg accgcctcgt catcaaggac ggcgtcctgg tcattgggct ctccgccctc
     4381 gccatgttga tttacaccaa ggcgtcggtg ggcctcctgg tggtcatgta cagcatcaac
     4441 gtgttcctga cgttttccct gtcccaagcc ggcatggtcc gccactgggt tcgggaccgg
     4501 ggcgacggct ggccgcgcaa attggcgatt aacgtcaccg gcttgatact gaccctggga
     4561 attttgatcg tgaccgtctt catcaagttc cgggaaggcg gttggatcac cctcgttatc
     4621 accggggtga tcgtggcctt ctgcctgtgg gtcaaccacc actaccggga caccgccaag
     4681 gccctgcacc atttggacga gatcctgacg cagatcccgc tccccgagga aatgcccgtc
     4741 gtcaagaaag tcccgggcaa acccgcggcc atcctgatgg tcaacggcta caacggcatg
     4801 ggaatccact cctttttggc gatccaccgg ctctttcccg ggcactttaa gaacttcgtc
     4861 tttctggcgg tg
//
LOCUS       DAOQLS010000070         4859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8155_length_4859_cov_3.941507, whole genome shotgun sequence.
ACCESSION   DAOQLS010000070 DAOQLS010000000
VERSION     DAOQLS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8155_length_4859_cov_3.941507"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK362_02085"
     CDS             <1..389
                     /locus_tag="PK362_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73663.1"
                     /translation="LTGLLSTAVARKAAPPWTFAATALLAALAITYFCPLEWFLGWSP
                     VPKALASLVLVGLPIFFAGLVFAEFFEDHPFSPRGFAWNLLGAVAGGLLEFLSMALGF
                     RALVLLAGAVYLTAFAIRPRRRGVPR"
     gene            386..967
                     /locus_tag="PK362_02090"
     CDS             386..967
                     /locus_tag="PK362_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC73664.1"
                     /translation="MTEGNFFPGTGSNPPEGIDAMLQTHGPRIFALAVRLCGNRTDGE
                     DLAQETFLQAFRRRDQFRGEADLGSWIYRICVNLWKNRVRYEKRRFFWQHRPLEIRDA
                     DGEVRPMEIADPSDATDGPAELDERQRLVRRALNALEPHERAVIVLRDMEDKSYEEIA
                     ACLDLPLGTVKSRIARAREALKEKLEPLLGEQP"
     gene            964..2301
                     /locus_tag="PK362_02095"
     CDS             964..2301
                     /locus_tag="PK362_02095"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HNC73665.1"
                     /translation="MTDANQNTPKERLSAYLDDALAPAERAAFEKELAASANLRRDLA
                     RLKSLRSILRSMPPPAPTETFYRNVRRAAEPRRWRFVLPPLAAAAAAALIMVGIRHND
                     RPTVALDALSPEGSTVARRSLAAKPRFLESGTDAETVRRAALPADRPSSDYNAAAAPS
                     EELRLRESGAVADKKDFPDVLAKQRLQAAEPRAERGDGMAPPTVSREEKEKLPAGPRP
                     AIGDRKGLLEKAKVAEFDSPVPAAPPLATLPAEDDGNPSRSLSEGFGGGTPAAGTGPT
                     RTQNALVRAAKPAPAAVGSVSKDEAALSAREGSKKSSIAGFRIPSKTAARAEWRGDDS
                     GVLQFRTVVVRDAAAWKTLWAEHAALRVPPPPAPDVDFSRDMVVGVFLGQKPSGGYEA
                     MLVDFRAGAEGATVTYQTATPDPDQSQITVLTAPYHLRVVPRTARPVRFKKIP"
     gene            2388..4775
                     /locus_tag="PK362_02100"
     CDS             2388..4775
                     /locus_tag="PK362_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73666.1"
                     /translation="MTRRLLPALLLAAWSAQAADTSDNARLKLPWDTIKSVLKIDRND
                     VRLTAAEFETLLKLTSAKPLPDYRLMEGDVVLPRAEFTRLVQSLVPPAPEAAGAYLPK
                     AVYRGRLENHAVRFAAALQLEIPRRPTRPLSVDVFPGGVAFEEILLDGKPARTDLSTG
                     RLTLVATEAGSHRVDLKYVVPLPDASAAQSFTVPVARTPITDWTLEIPENNLDIAVSP
                     ALHRDVSGAAGATRVRALLPPTDTVSATWNPLAPDAAKGPAQVYATVDHLLSVEEDAL
                     RLTGIVDLEVLHNTINRATLEIPEGFAVLNVRGEDLAEWQENAGRPATLDVPFRSARK
                     GRVAFEVDLERVIPGEKSTTTVAGLTVRGALRQRGFVGLELKSDAELPPPDTQGLDPQ
                     DPFRELPALLTARSARLLFGYRYLRPPFQIRLALSRHETVAVVSSVIDRAEGTTVLRE
                     DGKRVHQVVYYLRQAAKQFLSIRLPEGARLWNAFVDGAPVKPVMGSSGTVLLPLIRAG
                     RNPADEFPVEFVYFEKTTPLGFLGRETVHFPTPDVMVSRLRWTVVTPPDQGFHSFGHD
                     FEPEIAAPAAPAFLAANGVTRAFAAPDEPMIEESEGALKDRDGFDDKERLDQLSAAKP
                     MAKKIQTFRAEMNAPAPSVGGEWQGKGGGAKTDVSAVRGVMPVRVSVPRVGVQRVFAK
                     TLPESNALLPLVFYHGTAGAGRLLKLILVLGALGLMWIFRTPLGRGALWVRPRVEPWA
                     RRVPWTDPALGAFGAGVLSLGAAALSRGLFYISLIPFTWAVGRWILSALPRGDHR"
BASE COUNT          787 a         1701 c         1510 g          861 t
ORIGIN      
        1 tcctgaccgg tcttctttcc acggccgtgg ctcgaaaagc cgcccccccg tggaccttcg
       61 cggcgaccgc cttgttggcg gccctggcca tcacttattt ttgtccgctc gaatggttcc
      121 tgggctggag ccccgttccg aaagcgttgg cctcgttggt tctggtggga cttcccattt
      181 tctttgccgg cttggtcttt gccgaatttt tcgaagacca tccgttttcc ccccgcggtt
      241 ttgcctggaa ccttctgggc gcggtcgccg gcggtttgct tgagtttctt tccatggccc
      301 tggggttccg tgcgctggtg ctcttggcgg gggcggttta tctgaccgcc ttcgccattc
      361 gcccccgccg tcgaggagtt ccgcggtgac ggaagggaac ttttttcccg ggacggggtc
      421 caaccccccg gagggcatcg acgccatgct tcaaacccac ggacctcgca ttttcgccct
      481 ggccgtccgg ctctgcggca accggaccga cggggaggat ttggcccagg aaaccttcct
      541 ccaggcgttc cgccggcggg accaattccg gggcgaggcc gacctgggat cctggatcta
      601 ccggatttgc gtcaacctct ggaaaaaccg ggtgcgttac gaaaaacgac gtttcttctg
      661 gcagcaccgg cccttggaaa tccgggacgc ggacggcgag gttcgaccga tggaaatcgc
      721 ggacccctcc gacgccaccg acggtccggc ggaactggac gagcgccaac gcctcgtgcg
      781 ccgggccctg aacgccctgg agccccacga acgcgcggtg atcgtcctcc gggacatgga
      841 agacaaatcc tacgaggaaa tcgccgcctg cctggacttg cccctcggca ccgtgaaatc
      901 ccgcatcgcc cgggcccggg aggccctgaa ggaaaagctg gagccgcttt tgggagaaca
      961 gccatgaccg acgcgaacca aaacaccccg aaggaacgcc tctccgccta cctggacgac
     1021 gccttggcgc ccgccgagcg cgccgccttc gaaaaggaac tggccgcttc ggcaaaccta
     1081 cgccgggacc tggcgcgcct aaaatccctg cggtcgattc ttcgatcgat gccgcccccc
     1141 gcgcccacgg aaacctttta ccggaacgtg cgacgagcgg cggaaccccg gcgctggcgt
     1201 ttcgttcttc ctcccctggc cgcggcggcc gcggccgcct tgatcatggt cgggatccgc
     1261 cacaacgacc ggccgaccgt cgccttggac gccctttcgc cggagggatc gaccgtggcg
     1321 cggcggtcct tggccgcaaa accccgattt ttggaatccg ggacggacgc ggaaacggtt
     1381 cggcgggccg ctctcccggc ggaccggccg tccagcgatt acaacgccgc cgcggccccg
     1441 tcggaagaac tacgtcttcg ggaatccggc gccgtggccg acaaaaagga tttccccgat
     1501 gtcctggcca agcagcgtct ccaggccgcg gaaccccggg cggagcgggg ggacgggatg
     1561 gcgccgccga cggtgtcccg ggaggaaaag gaaaaacttc ccgccggacc tcgcccggcc
     1621 atcggagacc gaaaaggcct tttggagaaa gccaaggtcg ccgaattcga ttccccggtc
     1681 ccggccgcgc cccctcttgc gactctcccg gcggaagacg atggaaatcc ttcacggtct
     1741 ctttcggaag gattcggcgg gggaactccc gccgcgggga ccgggccgac ccggacccaa
     1801 aacgcactgg tccgggccgc caagcccgcg ccagcggccg tcggatccgt ttcaaaagac
     1861 gaagcggccc tgtccgcgcg ggaaggctcc aaaaagtcgt ccatcgccgg ttttcgaatt
     1921 ccttcaaaga ccgccgcccg ggcggagtgg cggggcgacg acagcggagt gcttcaattc
     1981 cgaacggtgg tcgtccggga cgccgccgcc tggaaaacgc tctgggcgga gcacgccgcc
     2041 ctccgggttc ccccaccgcc cgccccggat gttgattttt cccgcgacat ggtcgtgggc
     2101 gttttcctcg ggcaaaagcc gtcgggcggg tacgaagcga tgctcgtgga ttttcgggcg
     2161 ggggccgagg gcgcgacggt cacctatcaa acggcgaccc cggatcccga tcaaagccaa
     2221 attacggtcc tcaccgcccc ctaccacctt cgcgtcgttc cccggaccgc ccgtccggtt
     2281 cgatttaaaa agattcccta gccgggaact tttcccgggc gccggtgtct tccggttatg
     2341 aatcccttga ttcccgcgcc tttggaacct acgcttgggg gacgcccatg acacgccgac
     2401 ttctccccgc cctgctcctc gcggcgtggt ccgcccaagc ggcggacacg tcggacaacg
     2461 cgcgtcttaa actcccctgg gacaccatca agtccgttct gaaaatcgac cgcaacgacg
     2521 tccgattgac ggcggccgaa tttgaaaccc tcctgaaact cacaagcgcc aaacccttgc
     2581 cggactatcg cctcatggaa ggcgacgtgg ttcttccccg agccgaattc acccgcttgg
     2641 tccaaagcct ggtccccccg gcccccgagg ccgcgggggc ttatctgccc aaggcggttt
     2701 atcgggggcg gttggaaaac cacgccgtgc gtttcgccgc ggcccttcaa ttggaaattc
     2761 cccgccgccc gacgcgcccc ctctccgtcg acgtgtttcc gggcggggtg gcctttgagg
     2821 agatcctgct ggacgggaaa cccgcgcgaa cggatttgtc caccggccgt ttgaccctcg
     2881 tcgccacgga agcggggtcc caccgggtgg acttaaagta cgtcgtgccc ctgcccgacg
     2941 ccagcgcggc ccaatccttc acggtgcccg tggcccgcac gcccatcacg gattggacgc
     3001 tggaaattcc cgagaacaat ttggacatcg ccgtgtcccc ggcccttcac cgggacgtga
     3061 gcggcgccgc cggcgccacc cgggtgcggg ccctcctccc gcccacggac accgtctccg
     3121 cgacctggaa tcccctcgcc cccgacgccg ccaagggacc cgcccaggtg tacgccacgg
     3181 tggatcatct cctgtccgtg gaagaggacg ccctgcggtt gacggggatc gtcgatctgg
     3241 aagtcctgca caacaccatc aaccgggcca ccttggaaat cccggagggc ttcgcggtgt
     3301 tgaacgtccg cggcgaggac ctcgcggaat ggcaggaaaa tgcgggccgt cccgccacgc
     3361 tggacgttcc tttccggtcg gcgcggaaag gacgcgtggc ttttgaggtc gatttggaac
     3421 gcgtgatccc cggagagaaa tcgacgacca cggtcgccgg tctgacggtg cggggcgccc
     3481 tccggcaacg ggggttcgtc ggattggaat tgaaaagcga tgcggaattg cccccgccgg
     3541 acactcaggg attggacccc caggacccct ttcgggaatt gcccgcccta ttgacggcgc
     3601 gatccgcccg tctgctcttt ggctaccgct accttcgccc ccccttccaa attcgtttgg
     3661 ccctgtcgcg ccacgagacc gtggcggtcg tgtcctcggt catcgaccgg gccgaaggca
     3721 ccaccgtcct gcgggaagac ggcaagcgcg ttcaccaagt ggtttattat cttcgccagg
     3781 ccgccaagca gtttctgtcg atccgccttc ccgaaggggc gcgtctctgg aacgctttcg
     3841 tggacggggc cccggtcaaa cccgtgatgg gctcctccgg gacggtcctt ctccccttga
     3901 ttcgcgcggg ccgaaacccc gccgacgagt tccccgtgga gttcgtgtat ttcgagaaaa
     3961 cgaccccgct cgggttcctc gggcgggaaa cggtccattt cccgacgccg gacgtcatgg
     4021 tgagccgcct tcgctggacc gtcgtcacgc cgccggacca gggattccac tcgttcggcc
     4081 acgattttga acccgaaatc gcggccccgg cggctccggc tttcctcgcc gcgaacgggg
     4141 tgacccgggc cttcgcggcc cccgacgagc cgatgatcga agaatccgag ggcgccctca
     4201 aagaccggga cggtttcgac gataaggaaa ggctggatca actgagcgcc gcgaaaccga
     4261 tggccaagaa aattcaaact ttccgagcgg aaatgaacgc ccccgcgccg tcggtcggcg
     4321 gggaatggca aggcaaaggg ggcggcgcca aaacggacgt ttccgccgtc cgcggcgtga
     4381 tgcccgtgcg ggtgtccgtt ccccgggtgg gcgtacagcg ggtcttcgcc aaaacccttc
     4441 cggaatccaa cgcccttctc cccttggtgt tttatcacgg cacggcgggg gcggggcggt
     4501 tgttgaaact aatcctcgtc ctgggggccc tcggcttgat gtggattttc cggacccccc
     4561 tgggacgcgg ggccctctgg gtccggcccc gggtggaacc ctgggcgcga cgagtgccct
     4621 ggaccgatcc ggccctgggc gccttcggcg ccggagtcct ctccctgggc gcggccgctc
     4681 tgtcccgcgg actcttctat atttccctca tccccttcac ctgggccgtt gggcgctgga
     4741 tcctgtcggc cctgccccga ggagaccacc gatgatttat cgacgaatcg ccatgatcgg
     4801 cgtcctggtc ggcgccgccg tcgccgcgcg ggccgacgac ccctcccccc aatcccagg
//
LOCUS       DAOQLS010000071         4845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8193_length_4845_cov_4.204175, whole genome shotgun sequence.
ACCESSION   DAOQLS010000071 DAOQLS010000000
VERSION     DAOQLS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8193_length_4845_cov_4.204175"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="PK362_02105"
     CDS             complement(<1..1549)
                     /locus_tag="PK362_02105"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNC73667.1"
                     /translation="MAKKYVYFFGNGKAEGNGTQKDLLGGKGAGLAGMSSAGVPVPPG
                     YTITTETCRYYYANGKKLPKELAAQQKEAMGKLEKALGKKFGDNKNPILVSVRSGAKF
                     SMPGMMDTILNLGLNDEAVLGLADKTGNERFAWDAYRRFISMFGNVVMEIEKGEFEKE
                     LESVKHKKGVHLDTELGVADLKQVVDLLKKLVKRETGKDFPQDPWLQLEEARNAVFRS
                     WNNPRAITYRRLNKISDDIGTAVNVQAMVFGNMGNDSGTGVGFTRNPSSGVKEFFGEY
                     LTNAQGEDVVAGIRTPKPITELQNDMPQVFKQLKDITSKLEKHYRDMQDFEFTIEKGK
                     LFMLQTRNGKRTGIAAVRVAVEMVNEKLISKEEAILRIEPDQLAQLLAPVFDGKEKAA
                     AVKGGRFLAKGIDAGPGAAAGRVAFTSEKAVTMSKQGPVVLVRPETNPDDIEGMVVAE
                     GILTAIGGRTSHAAVVARGMGKPCVVGCGVLKIDLDAGVLAVNGTRVKEGDYLSIDGF
                     TGEVITGQ"
     gene            complement(1700..2692)
                     /locus_tag="PK362_02110"
     CDS             complement(1700..2692)
                     /locus_tag="PK362_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73668.1"
                     /translation="MTGRWVGGLLAGIVLSGAPVGAEAPEVRKVVEGRTFVFRDGKAY
                     ERVDGRETLFKILYDPTHVERCYRTRDGRVFARSEDGKEYPVLRSFADGFENAASLAD
                     LIGPARGWSAVTLQSPRAPTVAAYVALRNRILSGASNFLDNRVEPSTARAHAGRASLH
                     LYSAAPTRSMTCSKASLETGLAYFVRNDDFWFSGWYFIVGGRPVGLVDVESTYIDEGP
                     GPRVLLSEAGEPRVELKWADKPTYRAPPGVVVPRGEWFRLRVHYRLRDDDQGLVQLWL
                     NDEKIIDARGQTLPLPDTVLDRVEVGLTASPPGGPSELFLDDVAFGAEPPPGAR"
     gene            complement(2689..>4845)
                     /locus_tag="PK362_02115"
     CDS             complement(2689..>4845)
                     /locus_tag="PK362_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC73669.1"
                     /translation="DGAHPDLASRMWSNPGEVPGNGLDDDGNGFVDDAGGWDFANEDN
                     DPADDAGHGTHVAGTVAAAGDNGLGVVGVAFGARLMPVKGLDAGGGGYASDLAAALVY
                     AVDNGARIINASWGAPAKSALIEAAANYVRASGGLLVAAAGNSNGPVAYPAALDGVVA
                     VAATDEADAKADFSSFGPQVDLAAPGVDIRSTLPGGMYGTKSGTSMAAPHVAGVAALI
                     AALHPFYTPAQLTAALRLATDDVNTPGFDAATGAGRVNAARAVALRDLAAVHDPPAVA
                     ILYPSDGLTVAGPQGLRGTVSSPVGLGRVEVLADGAFRGIAQVDHGAWSFAADATGWS
                     GAHQWTVRAWDVFDQFAEATVTVTATTSAAPVPEIPAVYDPGWGAPACGVGVECDSVN
                     LLAGRGGLLGGVEPHAPNTLVSAPCADGNEGSYHLDESIDRLRIRSAAGGPLTAGGVA
                     TVDVTYWSYAGFDADRVALYYATSPAAPAWTPLVSFVPTAAGGQTLRTAMTLPDAPRG
                     VLRAVMTYQIPPDVCAGGDYTDHDDLVFEVAADTRPPVLSDVRVRPEPRRASFAWATD
                     ELSSAWLDVGPAGASPNGRVALPDLALTHAAVFNDLSPETVYEFTIASRDAGGRESLS
                     TGTFRTTAENGTSAPLAFPSPFRPGDGEARLLHLPPHKSVRLVTVSGRTIRTLPVDES
                     GEARWDGVGENGEPVPSGVYYVLPDGGRPFPLVIQK"
BASE COUNT          802 a         1585 c         1648 g          810 t
ORIGIN      
        1 cctggccggt gatgacctcg ccggtgaacc cgtcgatgga gaggtagtcg ccctctttca
       61 cccgggtgcc gttgacggcc aggacgccgg cgtccaagtc gattttcaag acgccgcagc
      121 ccaccacgca gggtttgccc atgccgcggg ccacgacggc cgcgtgggag gtgcgtccgc
      181 cgatggcggt caaaatgcct tccgccacga ccatgccttc gatgtcgtcg gggttggttt
      241 cgggacggac gaggaccacg gggccctgct tggacatggt caccgctttt tcggaagtga
      301 aggccacgcg gccggcggcg gcgccgggac cggcgtcgat gcccttggcc aggaagcgac
      361 cgcccttcac ggcggcggcc ttctctttgc cgtcgaagac gggggcgagg agctgggcca
      421 gctggtcggg ctcgatgcgg aggatggcct cttccttgga gatcaatttc tcgttgacca
      481 tctcgacggc gacgcggacg gcggcgatgc ccgtgcgttt gccgtttcgg gtctggagca
      541 tgaacagctt ccctttttca atggtgaact caaagtcctg catgtcgcgg tagtgcttct
      601 cgagcttgga ggtgatgtcc ttcagctgct tgaagacctg cggcatgtcg ttttgaagtt
      661 cggtgatggg cttgggcgtc cgaatgccgg ccaccacgtc ttcgccctgg gcgttggtca
      721 aatactcgcc gaagaattcc ttcacgcccg aagaggggtt ccgggtgaag cccacgccgg
      781 tgcccgaatc gttgcccatg ttgccgaaca ccatggcctg gacgttgacg gcggtgccga
      841 tgtcgtcgga gatcttgttc agacggcggt aggtgatggc gcgggggttg ttccagctgc
      901 ggaaaacggc gttccgggcc tcttccaatt gcagccaggg gtcctggggg aagtccttgc
      961 cggtttcgcg tttgacgagc ttcttgagca ggtccacgac ttgcttgaga tccgccacgc
     1021 ccagctcggt gtccaagtgg acgccctttt tgtgcttcac ggattcgagt tctttttcaa
     1081 attcgccctt ttcgatttcc atgaccacgt tgccgaacat ggagatgaag cggcggtagg
     1141 cgtcccaggc gaaacgttca ttgccggtct tgtcggccag accgaggacg gcctcgtcgt
     1201 tcaagccgag gttcaaaatc gtgtccatca tgccgggcat ggagaacttc gcgcccgagc
     1261 ggaccgagac caggatgggg ttcttgttgt ccccgaattt cttgcccagg gccttttcca
     1321 gtttgcccat ggcttccttc tgctgggcgg ccaattcctt gggaagcttt ttgccgttgg
     1381 cgtagtagta gcggcaggtt tcggtggtga tcgtgtagcc cgggggcacg ggcacgcccg
     1441 cgctggacat gccggccaaa ccggcgccct tgccgcccaa aaggtctttc tgcgttccgt
     1501 tgccttcggc cttgccgttg ccgaagaaat agacgtattt tttcgccatg acagtgaaac
     1561 tccttagaga gagacgagtg tattgaacgc gagggtgagg cggagagctg cgggacttgg
     1621 aaaagcccgg aaggccgatc ccgagggaag gcccccagcg cgttacgcgg gggattttag
     1681 caaagccgga gaggctccgt caacgcgccc cggggggcgg ttcggccccg aaggccacgt
     1741 cgtccaggaa cagttccgaa gggccgccgg gcggactggc cgtcagcccc acctcgaccc
     1801 ggtccagcac ggtgtccggc aaggggaggg tttggccccg ggcgtcgatg atcttctcgt
     1861 cgttcaacca caattgaacg aggccttggt cgtcgtcccg aagccggtag tgaacccgga
     1921 gtcggaacca ttccccccgg ggaacgacga cccccggcgg ggcccggtag gtgggtttgt
     1981 cggcccactt caactccacc cggggctcgc ccgcctccga caggagcacc cgagggccgg
     2041 ggccttcgtc gatgtaggtg ctttccacgt cgacgagccc cacgggtcgt ccgccgacga
     2101 tgaaatacca acccgaaaac caaaaatcgt cgttccgaac gaaataggcc aatcccgttt
     2161 ccaaagacgc tttggaacag gtcatggacc gcgtcggggc ggcggaataa agatggaggc
     2221 tcgcccggcc ggcatgggcc cgggcggtgg aaggctcgac ccggttgtcg agaaaattcg
     2281 aggcgcccga caaaatccga tttcgaagag cgacgtaggc ggccacggtg ggggcccggg
     2341 gggattgaag ggtgacggcc gaccaccccc gggcggggcc gattaaatcc gccaaagaag
     2401 cggcgttctc gaaaccgtcc gcaaacgatc gaagaacggg gtattccttt ccgtcctccg
     2461 accgggcgaa cacccgcccg tcccgggtcc ggtagcaacg ttcaacgtgg gtggggtcgt
     2521 agaggatttt aaagagggtc tcacgcccgt cgacccgctc gtaggccttt ccgtcccgga
     2581 aaacgaaggt tcgcccctcg acgacctttc gaacttccgg cgcttcggcc ccaacggggg
     2641 cgcccgaaag aacgatcccc gcgagaagcc ctcccaccca acggccggtc atttctgaat
     2701 cacaagagga aagggccgcc cgccgtccgg caggacataa tagaccccgc tcggcacggg
     2761 ctcgccgttt tccccgacgc cgtcccaacg ggcctcgccg ctttcgtcca cgggaagcgt
     2821 ccggatggtt cggccggaga cggtcacaag gcggaccgat ttgtgcgggg gcaggtggag
     2881 gagccgggcc tcgccgtcgc cgggacgaaa gggggaaggg aatgccagcg gcgcggaggt
     2941 cccgttctcc gccgtcgtcc ggaaggtccc ggtggagaga ctttcccggc cgccggcgtc
     3001 ccgggaagcg atcgtaaatt cgtagaccgt ttccggggac agatcgttga acaccgccgc
     3061 gtgggtcaag gccaaatcgg gcagcgcgac gcggccgttc ggcgacgccc cggcgggacc
     3121 gacgtccaac cacgcggagg aaagctcatc ggtggcccag gcgaaggagg cccggcgggg
     3181 ttcggggcgg acccgcacgt ccgagagaac cggcggccgg gtgtccgccg ccacctcgaa
     3241 caccaaatcg tcgtggtccg tgtaatcgcc tccggcgcac acgtccgggg ggatttggta
     3301 cgtcatgacg gcccgaagga ccccccgggg cgcgtcggga agcgtcatgg ccgttcgaag
     3361 ggtctgcccc ccggccgccg tggggacgaa ggacaccagg ggggtccagg ccggggccgc
     3421 cggcgaagtg gcgtaataca gggccacccg gtcggcgtcg aacccggcat acgaccaata
     3481 ggtgacgtcg acggtcgcca ccccccccgc cgtcaacggc ccgccggcgg cgctccggat
     3541 ccgcaggcga tcgatggatt cgtccagatg ataggatccc tcgttgccgt cggcgcaggg
     3601 cgcggagacg agggtgttgg gagcgtgggg ttcgacgccg cccaggagtc ccccccggcc
     3661 cgccaataag ttaaccgaat cgcattccac ccccacgccg caggcgggcg ccccccaccc
     3721 cggatcgtaa acggcgggga tttccggaac cggggcggcg gacgtcgtcg cggtcaccgt
     3781 cacggtcgcc tcggcgaatt gatcgaacac gtcccaggcg cgcacggtcc attggtgggc
     3841 cccggaccac ccggtggcgt cggccgcgaa ggaccaggcc ccgtggtcga cctgggcgat
     3901 tccccggaac gccccgtccg ccaacacctc cacccggccc aaccccaccg gggaggacac
     3961 cgttccccgc agcccctggg ggccggccac cgtcaacccg tcggaaggat acaggatggc
     4021 gacggccggg ggatcgtgga cggcggccaa atcccgtagg gcgacggccc gggcggcgtt
     4081 gacccgtccg gcgcccgtgg ccgcgtcgaa tccgggggtg ttgacgtcgt cggtggccaa
     4141 gcggagggcc gcggttagtt gggcgggggt gtaaaacggg tgaagcgccg cgatcaaggc
     4201 ggccaccccc gccacgtggg gcgcggccat gctggtcccg cttttggtcc cgtacatccc
     4261 tccgggcagg gtggaccgaa tgtccacgcc cggagcggcc aggtccactt gggggccaaa
     4321 actcgagaaa tcggccttgg cgtcggcctc atcggtggcg gccacggcga ccaccccgtc
     4381 caaggccgcc gggtaggcca ccgggccgtt gctgttgccg gccgccgcca ccaacaggcc
     4441 gccgctcgcc cgcacgtagt tcgcggcggc ttcgatcaac gcgcttttgg cgggagcccc
     4501 ccagctggcg ttgatgattc gggcgccgtt gtccacggcg tacaccagcg cggcggccaa
     4561 atcggaggcg tatccgccgc ccccggcgtc caaccccttg acgggcatca gccgggcgcc
     4621 gaaggccacc ccgacgacgc cgagaccgtt gtcgcccgcg gcggcgacgg tccccgccac
     4681 gtgggtgccg tggccggcgt cgtcggccgg gtcgttgtcc tcgttggcaa aatcccaccc
     4741 gccggcgtcg tccacaaacc cgttgccgtc gtcgtccaaa ccgttgcccg gaacttcgcc
     4801 ggggttggac cacattcgac ttgccaagtc cggatgggcc ccgtc
//
LOCUS       DAOQLS010000072         4842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8201_length_4842_cov_3.188009, whole genome shotgun sequence.
ACCESSION   DAOQLS010000072 DAOQLS010000000
VERSION     DAOQLS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8201_length_4842_cov_3.188009"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK362_02120"
     CDS             complement(<1..189)
                     /locus_tag="PK362_02120"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HNC73670.1"
                     /translation="MKKRVHLIFSPTLVQKPITWELSKKFDIVFNVRRAKITETVGES
                     VLELEGDGPTLDAAVAWLK"
     gene            complement(356..634)
                     /locus_tag="PK362_02125"
     CDS             complement(356..634)
                     /locus_tag="PK362_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC73671.1"
                     /translation="MAVKVRIPAPLRRLTKDQAVVEVEGATIEQVIAGLEKGFPGLKE
                     RLCDETGQIRRFINVFVNGEDIRFKDGAKTAVAAGAEVSIIPAIAGGK"
     gene            complement(658..>1305)
                     /locus_tag="PK362_02130"
     CDS             complement(658..>1305)
                     /locus_tag="PK362_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC73672.1"
                     /translation="GSKTLGFEVAEQLGWRAPDHCVVPMASGSLLTKIHKGLQEFQEL
                     GLIPKQKTRMSGAQAAGCAPVVTAVREGTDIVKPVRPNTIAKSLAIGNPADGPYAAKI
                     IKGTGGHAEDVTDEEVVDGIKLLARTEGLFTETAGGVTIGVLKKLAASGKIASDETVV
                     AFITGSGYKTIEAVAGRTAVLHDIPPTLEAFRSLHQRVKGARSVPGTPPLESVGA"
     assembly_gap    1306..1405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1406..2093)
                     /gene="thrC"
                     /locus_tag="PK362_02135"
     CDS             complement(<1406..2093)
                     /gene="thrC"
                     /locus_tag="PK362_02135"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC73673.1"
                     /translation="MTSKIKGLKCKECGQIYPALATHVCEFCFGPLEVDYNYDVIRPG
                     LTRAKIESGPRTLWRYWDLLPVESKNLVSIHEGFTPLFHAKHLGRELGLKNLYIKNDS
                     VNPTYSFKDRVVSVALTRAKELGFDTVACASTGNLAGAVAAYGASSGLKTFVFIPADL
                     EQGKIIGAGVYDPVLVGVRGNYDEVNRLCAEVADAFKWAFVNVNVRPYYAEGSKTLGF
                     EVAEQLGWRAP"
     regulatory      complement(2176..2290)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2343..2795)
                     /locus_tag="PK362_02140"
     CDS             complement(2343..2795)
                     /locus_tag="PK362_02140"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC73674.1"
                     /translation="MELDRRSGHRIPVPAQFGGLVSFAPVGLLGRLKIARDGVVENLS
                     TEGLNAFVHGPLRKGNKLWVTFYAEGPLRGVRLSGRVVWAEKEAGGTRVGLRFLSVPA
                     EATTVIGRWAYDHQVCEAGITFALKNICKTTCAYWSLCEKSIKIRPAA"
     gene            complement(2981..>3148)
                     /locus_tag="PK362_02145"
     CDS             complement(2981..>3148)
                     /locus_tag="PK362_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HNC73675.1"
                     /translation="WVDEVIAANAKIVADIKGGKPSAVGSLVGQVMKKSSGRANPQTV
                     QRLLKEKLSVN"
     assembly_gap    3149..3248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3249..4312)
                     /gene="gatB"
                     /locus_tag="PK362_02150"
     CDS             complement(<3249..4312)
                     /gene="gatB"
                     /locus_tag="PK362_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNC73676.1"
                     /translation="MTAYETVIGLEVHVQLKTESKIFCSCPTTFGAEPNTQICPVCAG
                     YPGVLPVLNKKVVEGLVRAALALHCGINRHSIFARKQYFYPDLPKNYQISQYELPLAI
                     NGHLDIAVPPAGPRRIRIHRIHLEEDAGKLLHAIGNRALDYSLVDLNRTGVPLMEIVS
                     EPDLRSPEEASAYLDTLRNILRYVGVSDCDMEKGSMRCDANVSLRPVGTETLGTKAEV
                     KNMNSMKSVRDAIAHEVLRQTALLESGGRVVQETRLWSQDTGTTQSMRSKEEAHDYRY
                     FPDPDLVPLDLSTEFITNLQSQLPELPEARRARYEKELGLSPYDAGVLTAERGLSDYF
                     DRGLAGFPPAERGAAAKPLS"
     gene            complement(4309..4773)
                     /locus_tag="PK362_02155"
     CDS             complement(4309..4773)
                     /locus_tag="PK362_02155"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC73677.1"
                     /translation="MMSKGDSTTQGDPAVPGEEVSAGGIVLGPEGLLMIKVRNLEDNV
                     VWTFPKGHLEAGETPQQAALREVLEETGWKCRVIGDLGEVRYKFKRDLRPVNKTVHWF
                     YMTPEDRPGRPDPDEVLDCRWYPLPEAASLVVYKSDKMILSLLKKKAGEALP"
BASE COUNT          815 a         1462 c         1440 g          925 t
ORIGIN      
        1 ctttaaccac gccacggcgg cgtccagggt gggaccgtcg ccttcgagtt ccaaaacgct
       61 ttcgccgacc gtttcggtga tcttcgcccg gcgcacgttg aagacgatgt cgaatttttt
      121 gctcagctcc caggtgatcg gcttttggac caacgtggga gaaaaaataa ggtgcacgcg
      181 ttttttcacg aaccgggact ccgatcttga tgtcgcgacc aaaacgacct ggccggatcg
      241 cctcgcccgc gggcgaatcg cggtactcgg tcaggcggcc gcccccgtgg aacggcgggc
      301 gtggaattca gccgacgact tccgcccgat ccttcgggcg gtccaacgaa aatctttatt
      361 ttcccccggc aatcgccggg atgatgctga cttcggcgcc ggcggccacc gcggtcttcg
      421 ccccgtcttt gaagcggatg tcctcgccgt tgacgaacac gttgatgaaa cgtcgaatct
      481 gccccgtctc gtcgcagagg cgttccttga gcccggggaa ccctttttcc aaaccggcga
      541 tgacctgctc gatggtcgcg ccctccacct ccaccacggc ctggtccttc gtcaagcgcc
      601 tcaacggggc gggaatgcgc acttttacgg ccataacaaa gtctccttta gtgtacgtca
      661 ggccccgacg gattcaaggg ggggcgttcc cggcaccgac cgcgccccct tgacacgctg
      721 gtgcagggac cggaaggcct ccaacgtggg aggaatatcg tgcaagaccg cggtgcgccc
      781 ggcgacggct tcgatggttt tgtagccgct gccggtgatg aaggccacca ccgtctcgtc
      841 ggaggcgatt ttaccactcg ccgccagctt tttcaacacg ccgatggtca cccccccggc
      901 ggtttcggtg aagaggccct cggtgcgggc caggagcttg atgccgtcga ccacttcctc
      961 gtcggtcacg tcctcggcgt ggccgccggt ccccttgatg atcttggccg cgtagggtcc
     1021 gtcggcgggg tttccgatgg ccagggattt ggcgatggtg ttggggcgga cgggtttcac
     1081 gatgtcggtc ccctcccgga cggcggtcac cacgggggcg cagccggcgg cctgggcccc
     1141 ggacatgcgc gttttttgtt tgggaatcaa tcccaattcc tgaaattcct ggagcccctt
     1201 gtggattttg gtcaacaacg aaccgctggc catggggacc acgcagtggt ccggcgcccg
     1261 ccagcccaat tgctccgcca cttcgaatcc cagggttttg gagccnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnccggc gcccgccagc ccaattgctc cgccacttcg
     1441 aatcccaggg ttttggagcc ttcggcgtaa taggggcgca cgttgacgtt gacgaaggcc
     1501 cacttgaagg cgtcggccac ctcggcgcag aggcggttca cctcgtcgta gttcccccgg
     1561 acgcccacca gaacggggtc gtaaacgccg gccccgatga ttttgccctg ctccaaatcc
     1621 gctgggatga aaacaaaggt tttaagcccc gaggaggcgc cgtaggcggc caccgccccg
     1681 gccaagtttc ccgtggaggc gcaggccacg gtgtcgaacc cgagctcctt ggcccgcgtc
     1741 aaggccacgc tcacaacccg gtccttgaag gaataggtgg ggttgaccga atcgttcttg
     1801 atgtaaaggt ttttgaggcc caattcccgg cccaggtgtt tggcgtggaa gagcggcgtg
     1861 aacccttcgt gaatcgagac caaattcttt gattccacgg ggaggagatc ccaataacgc
     1921 cacagggtcc ggggcccgga ttcgatcttg gcccgggtca atccgggacg aatgacgtcg
     1981 tagttgtaat cgacttccaa gggaccgaag caaaattcgc agacgtgggt ggccagggcg
     2041 gggtagatct gcccgcattc cttgcacttc aatcctttga ttttcgacgt catgccgcgt
     2101 tctccttggc cggaccttcg gcgcaaaaaa aatccccgag gagagatctc cacggggaac
     2161 ccgtttcttc gtctcctctt atcgttcccc tcgcgcgggg ccggcgttgg cacctgtccc
     2221 gaatccgata acgattcggc ggttgccgtg ggatcatcgg gcgttgtccc tcccccactc
     2281 tggataagag tacaagggct tttctaggaa agtcgcctca ttccgtcaag attcgcccga
     2341 gctcacgccg cggggcgaat cttaatggat ttttcgcaga gggaccaata ggcgcaggtg
     2401 gttttgcaga tgtttttgag ggcgaaggtg atccccgcct cgcacacttg gtggtcgtag
     2461 gcccaacggc cgatgacggt cgtggcctcg gcgggcaccg acaggaagcg caggcccacc
     2521 cgggtgcccc cggcctcttt ttccgcccac accacccggc cgctcaagcg aacgccccgc
     2581 agggggcctt cggcgtaaaa ggtgacccac agtttgtttc ccttccgcag gggaccgtgc
     2641 acaaacgcgt tcaagccctc cgtggacaga ttttccacca ccccgtcccg ggcgattttc
     2701 agccgcccca ggagccccac gggggcaaac gacaccaacc cgccgaattg ggcggggacg
     2761 gggattcgat gaccactgcg acgatccaat tccatgacag gcctcccacg gaatcgcggc
     2821 gcccgcccaa cagggacggg accgaggatt ccaaccatcg ttgtgccgat tgtgtcgcgc
     2881 gaggtgtggg atgagggatg ggggatgaaa aaaggccggt cgcgccggcc cctaagctta
     2941 ggcccgaatc cgcggaattg ccagtcccgg cggagacggg ttaattgacg gagagttttt
     3001 ccttgaggag gcgctgaacg gtttgggggt tggcccggcc cgaggacttc ttcatcacct
     3061 gacccaccag cgaccccacg gcgctgggtt tccccccctt gatgtcggcc acgattttgg
     3121 cgttggcggc gatcacctcg tccacccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnntt ggacagcggc ttggccgccg ccccccgttc ggccggcggg aaaccggcca
     3301 gaccccggtc gaaataatcc gacaaccccc gttcggccgt caaaacgccg gcgtcgtaag
     3361 gagagaggcc gagctctttt tcatagcggg cgcgccgggc ctcgggcagt tccggcaact
     3421 gggactgtaa attcgtgatg aattccgtcg ataaatccag gggcaccagg tccgggtccg
     3481 gaaaataacg gtaatcgtgg gcctcttcct tggaccgcat ggattgggtc gtcccggtgt
     3541 cctggctcca aagccgggtc tcctgaacca cccggccgcc ggattccagg agggccgtct
     3601 ggcgaaggac ttcgtgggcg atggcgtccc ggacgctctt catggaattc atgtttttca
     3661 cttcggcctt ggtgcccagg gtttcggtcc ccacgggccg aagggacacg ttggcgtcgc
     3721 aacgcatgga ccccttctcc atgtcgcagt cggagacgcc gacgtaccgc aggatgttcc
     3781 gcagggtgtc caggtaggcg ctggcctcct cggggcttcg aaggtcgggt tccgagacga
     3841 tttccatcaa gggcacgccc gtgcggttca aatcgacgag ggaataatcc aaggcccggt
     3901 tcccgatggc gtgcaggagt tttccggcgt cctcttccag gtgaatccgg tgaatgcgga
     3961 tgcggcgggg gcccgcgggg ggcaccgcga tgtccagatg gccgttgatg gccaggggca
     4021 gttcgtactg ggaaatctgg tagttcttcg gcaggtcggg gtaaaaatac tgttttcggg
     4081 cgaaaatgga atggcggttg atgccgcaat ggagcgccag cgccgcccgt accaacccct
     4141 ccacgacctt cttgttgagc acggggagga cgccgggata cccggcgcac acggggcaga
     4201 tctgggtgtt gggctccgcg ccgaaggtgg tcgggcaaga gcagaagatt ttgctttcgg
     4261 ttttgagctg gacgtggact tccaacccga tcaccgtctc atacgcggtc acggcaaggc
     4321 ctcccccgct tttttcttca gcaacgacaa aatcattttg tccgatttgt acaccacgag
     4381 gctcgcggcc tccggcaacg ggtaccaccg gcaatccagc acctcgtcgg ggtccggccg
     4441 gccgggccgg tcctcgggag tcatgtaaaa ccagtggacc gttttgttca ccggtcgcag
     4501 gtcccgtttg aatttgtaac gcacttcgcc caggtccccg atgacccggc atttccagcc
     4561 ggtttcctcg agcacttccc gaagggcggc ctgttggggc gtttccccgg cctccagatg
     4621 acctttggga aaggtccaca cgacgttgtc ctccaggttc cggaccttga tcatcaacag
     4681 gccttcggga ccgaggacga tgccgcccgc cgatacttcc tccccgggga ccgcggggtc
     4741 gccttgggtg gtggaatcgc ctttggacat catcgcttgc gcacctcgta ccaggtcggg
     4801 gccgcgctcc gctgccggag tcgccagggc atcaacaaaa at
//
LOCUS       DAOQLS010000073         4839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8210_length_4839_cov_3.983696, whole genome shotgun sequence.
ACCESSION   DAOQLS010000073 DAOQLS010000000
VERSION     DAOQLS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8210_length_4839_cov_3.983696"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK362_02160"
     CDS             <1..195
                     /locus_tag="PK362_02160"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HNC73678.1"
                     /translation="YLHLFEAKLRLPPGENDFSVWLSADWGDPSLAEKIRGLDLSLHT
                     LEEIKNKMVALLRSSESSHA"
     gene            188..1411
                     /locus_tag="PK362_02165"
     CDS             188..1411
                     /locus_tag="PK362_02165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC73679.1"
                     /translation="MRRLEEYRAVVGSDVLEELQLLANPLSGKRVVNINSTAVGGGVA
                     EILNGMIPLLKELGVDARWDVLKGGEAFYVVTKKFHNALHGDPVRFEPADFRVYEETV
                     EGNAALMALDSDVVYVHDPQPASLVQKRAAWKNRWIWRCHIDLTRREQVLWDYLRPVI
                     ESYDAAVFSSPAFAQNLPIPQVLIAPSIDPLSDKNRPLEDKEVKAVAERLGVPLDKPL
                     ITQVSRFDRLKDPVGVIEAFQMARKEVPARLLIVGGAADDDPEGMEVLAEVQEKAKGD
                     ADILVLNLPPTSHLEINALQRASTVVVQKSLREGFGLTVSEALWKNIPVIGGAVGGIP
                     QQVLHEHTGLLVTSVPEAAKAMARLLKEPEWAKNLGRNGHQHVRQNFLLTRHLRDYLL
                     LFHSVLNPGEHIVRL"
     gene            1561..2304
                     /locus_tag="PK362_02170"
     CDS             1561..2304
                     /locus_tag="PK362_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73680.1"
                     /translation="MNTKAFRWGLGLGLMALLAWPVAARERSRGGTFQGNRGGGTWAS
                     NTTRERGQMNRSTTVQSERGTAQRNVQRNWDRSSGKGTYSSETTGPGGKTVTRQGTVE
                     KTGEGSYHQTGTITGPQGHTTDVDRTSTRNDDGSRTVNTTYTNEKGETKTVDKTITKT
                     EDGRSMTGSYQTSTGKSGTIESKTTVEDGKIKTTGSVTNQDGKTWSRTAETSKTDTGL
                     HRDIAVTNPEGKTRDRSVDIQVTTKTVTP"
     gene            complement(2378..2806)
                     /locus_tag="PK362_02175"
     CDS             complement(2378..2806)
                     /locus_tag="PK362_02175"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNC73681.1"
                     /translation="MILNVVIGALLLGGWAAGAHQRRLLEGKEIVQGRVVELPVGHGS
                     KGGTIYGIRAEFKDRGGRAHTYQSGWKSSNPGYAVGDPIRIYYDPQNPDRCGIATFGM
                     RYGLAAGLLLAGILLVAVRWGLQAGQSWFEQAYPLTLGPR"
     gene            complement(2814..3884)
                     /locus_tag="PK362_02180"
     CDS             complement(2814..3884)
                     /locus_tag="PK362_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNC73682.1"
                     /translation="MSDIKPLLAKLAASDASDLHLCAGSPPCYRLNGRIEPVDPADKP
                     LEFRDLEQMIFHVLTEDQQARLMADHELDFSLGFSEVGRFRGNVHRQRGSWAVVFRKI
                     PPEIPSMIELGLPEIAKTLALKESGLVLVTGPTGSGKSMTLAAMIETINRERACHVIT
                     VEDPIEFLFKNQKALIKQREIGSDTDGYTTALRHSFRQDPDVLMIGEIRDRETISMAI
                     TAASTGRLVLGTLHTMDAVSTVDRLVESFPADQQNLVRNQIAGSLEGVISQQLVRKSH
                     GTGRVLAVEILVSTPSVRNQIRTGKTFQVISDIETGKRFGMQSMNQALQTLVTTDYIK
                     LEEAIRHTRFPEQLLQKLGVPQ"
     gene            complement(3881..4333)
                     /locus_tag="PK362_02185"
     CDS             complement(3881..4333)
                     /locus_tag="PK362_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73683.1"
                     /translation="MSLLHLGGAIAGAFRAEDFLADGPLRRWPAALESPLWTTAVLSA
                     ALFLVFWPPVRWLRRGLRRWSDADFWKSQFALLDGLGKLSRGIATLLAVLVLLCLLGN
                     DGVLAGGSWLKTVFFLFALLLAGVLSVIVGLYGWLAAQWNELRSEKTP"
     gene            4517..>4839
                     /locus_tag="PK362_02190"
     CDS             4517..>4839
                     /locus_tag="PK362_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC73684.1"
                     /translation="MRFLVTGAGGLIGANVSLTLAQRGHDVHALDHFAVGRRENLADF
                     KGTVHTGDIRDFDFAALGRFDGVFHQAAITDTTVMDEKLMRDVNVHAFVRILEAAHRT
                     GTPKVV"
BASE COUNT         1036 a         1492 c         1438 g          873 t
ORIGIN      
        1 taccttcatc tgtttgaagc caaactgcgc ctgccgcccg gggaaaacga tttctccgtt
       61 tggttatcgg cggattgggg cgacccgtcc ttggcggaaa agatccgcgg cctggatttg
      121 tcccttcaca cgctcgagga gatcaaaaac aaaatggtcg cgttgcttcg ttcgtcggag
      181 tcgtcccatg cgtaggcttg aggaataccg ggccgtcgtg ggttcggacg ttttggagga
      241 attgcaattg ttggccaacc ccctgtccgg aaaacgggtc gtcaacatca attccacggc
      301 ggtgggaggg ggcgtggccg aaatattaaa cgggatgatc cctcttttga aggaattggg
      361 ggtggacgcc cgatgggatg tgttgaaggg cggggaggcg ttttacgtcg tcacgaagaa
      421 attccacaac gcccttcacg gggaccccgt tcgcttcgaa cccgcggatt ttcgggttta
      481 cgaggagacc gtcgagggga acgcggcctt gatggccctg gattccgacg tggtctacgt
      541 ccacgacccc cagcccgctt ccctcgtcca gaaaagggcc gcttggaaaa accgttggat
      601 ctggcgctgc cacatcgacc tcacgcggcg ggagcaggtc ctatgggatt atcttcgtcc
      661 ggtgattgaa tcgtacgacg cggccgtgtt ttcgtccccg gcctttgccc aaaaccttcc
      721 gatcccccag gtgctgatcg cgccgtccat cgacccgttg agcgacaaga accggcccct
      781 cgaggacaag gaagtcaaag ccgtcgccga gcgcctcggc gtgccgctgg acaagccctt
      841 gatcacccag gtttcccggt ttgaccgcct caaagacccg gtcggcgtca tcgaagcctt
      901 tcaaatggct cggaaggaag ttcccgcacg acttttgatt gtcgggggcg cggccgacga
      961 cgaccccgaa gggatggagg tcttggcgga ggtccaggaa aaagccaagg gcgacgcgga
     1021 catcctggtt ctaaatcttc cccccaccag ccatctcgaa atcaacgcgc tccaacgcgc
     1081 ctcgaccgtc gtcgtccaga agtcgcttcg ggaaggtttc ggtttgaccg tttcggaggc
     1141 cctgtggaaa aacattccgg tgatcggcgg cgccgtgggc gggatccccc aacaggtgct
     1201 ccacgaacac acgggactgc tggtcacttc cgtgcccgag gcggccaagg ccatggcgcg
     1261 ccttttgaag gagccggaat gggcgaaaaa tttaggccgg aacggccatc aacacgtccg
     1321 gcaaaatttc ctcctcaccc gccatttaag agattatctc ctgctgtttc attccgtctt
     1381 gaacccgggg gaacacatcg tccgccttta agcgggaaat tcccccgaga aggcgttttc
     1441 tccggaagag gcatttttgg gagccggcgg gcgacacttt tgtcggatcg ccgtcgtcca
     1501 atggatacaa cggccgaatc gccggccgcg aaacgcgcac gaacgccagg gaggaatgtc
     1561 atgaacacaa aggcttttcg gtggggtttg ggactgggtt taatggcctt attggcctgg
     1621 cccgtggccg ctcgggaacg ttcccggggt ggaacctttc aagggaaccg cggcggtggc
     1681 acctgggctt cgaacacgac gcgggagcgg ggacaaatga accggtccac caccgttcaa
     1741 tcggaacggg gaaccgcgca gcgaaacgtc caacgtaatt gggaccggtc ctccggaaag
     1801 gggacttatt catccgaaac gaccgggccc ggcggaaaaa cggtgacccg ccagggcacc
     1861 gtggagaaaa cgggcgaagg ttcctaccac caaaccggaa ccatcaccgg cccccagggc
     1921 cacacaaccg acgtggaccg gacctccacc cggaacgacg acggcagccg caccgtgaat
     1981 accacctaca ccaatgaaaa gggcgagacg aaaaccgtgg acaaaacaat caccaaaacc
     2041 gaagacggac gctccatgac ggggagctac cagaccagca cgggcaaaag cgggaccatc
     2101 gaatccaaga ccacggtcga ggacgggaaa attaagacca ccggctccgt gaccaaccag
     2161 gacggaaaaa cctggagccg gacggcggaa acctccaaga ccgacacggg ccttcaccgg
     2221 gacatcgccg tcaccaaccc ggaaggtaaa acccgggatc ggtccgtgga catccaggtc
     2281 accacgaaaa ccgtcacccc gtaaattcct tttcgaaacg ccaaaggccg gcggcccccc
     2341 gagggggaac cgccggcctt ttatttttaa atcgtggcta acgcgggccg agcgtcaacg
     2401 gataggcttg ctcaaaccag gactgccccg cctgaagacc ccaccgcacg gccaccagga
     2461 gaatccccgc caagagcaaa cccgcggcca atccgtaacg cattccgaag gtcgcgatgc
     2521 cgcaccggtc cgggttttgc gggtcgtaat agatccgaat ggggtccccg acggcgtaac
     2581 ccgggttgga ggatttccaa ccggattggt aggtgtgggc gcggccgccg cggtccttaa
     2641 attccgcccg aataccgtaa atcgttccgc ctttggaacc gtggcccacc ggaagctcca
     2701 cgactcgccc ctgaacaatt tctttccctt ccaacaatcg gcgttggtgg gcgccggcgg
     2761 cccacccgcc caggagcaac gctccgatca cgacgttgag gatcatattc cccctactgg
     2821 ggcaccccca acttctgcag gagctgttcc ggaaaccggg tgtgccgaat ggcctcctcc
     2881 aatttaatgt aatccgtcgt caccagggtc tgaagggcct ggttcatgga ctgcatgccg
     2941 aaccgcttgc cggtctcgat gtcgctgatg acctgaaagg tttttcccgt tcgaatctgg
     3001 ttgcgaaccg acggcgtgga caccaggatt tccacggcca gcacccggcc ggtgccgtgg
     3061 gactttcgaa cgagctgctg ggagatcacg ccttcgaggc tcccggcgat ctggttgcga
     3121 accaagtttt gctgatcggc gggaaaactc tccaccagcc ggtcgacggt ggacaccgcg
     3181 tccatggtgt ggagcgtccc caacaccaaa cggcccgtgc tcgcggccgt gatggccatg
     3241 gaaatggttt cccggtcccg aatctccccg atcatcagca cgtccgggtc ctggcggaag
     3301 gaatgacgaa gggccgtcgt gtaaccgtcg gtgtcggagc cgatctcccg ctgtttgatc
     3361 agcgcctttt gatttttgaa aaggaattcg atggggtcct ccacggtgat cacgtggcag
     3421 gcccgttccc ggttgatggt ttcgatcatg gccgccaggg tcatggattt cccgctgccg
     3481 gtggggcccg tgacgagcac caagccggat tccttcagcg ccagggtctt ggcgatctcg
     3541 ggaaggccca attcgatcat ggaggggatt tccgggggga tcttccggaa gacgacggcc
     3601 caactgcctc gctggcggtg gacgttgccc cgaaaacggc ccacctccga aaatcccagg
     3661 gaaaaatcca attcgtggtc cgccatgaga cgggcctgct ggtcctcggt cagaacatga
     3721 aaaatcatct gctccaggtc ccggaattcc aggggcttgt cggccggatc cacgggttcg
     3781 atgcggccgt tcaggcggta gcagggcggc gacccggcgc acaggtgaag gtcggaggcg
     3841 tcgctggcgg ccaatttggc caaaaggggt ttaatatcgc tcatggggtt ttctccgatc
     3901 gcaattcatt ccattgggcg gccaaccacc cgtacaaccc cacgatcacg ctgagaaccc
     3961 ccgccaggag caaggcaaaa aggaaaaaaa ccgttttcaa ccaggaaccg cccgccaaaa
     4021 cgccgtcgtt tcccaacaga cacaagagca ccagaacggc caacagcgtg gcgatgcccc
     4081 ggctcaattt tcccagcccg tccaggaggg cgaattggga tttccagaaa tcggcgtcgc
     4141 tccaccgtcg aagtccccgc cgaagccacc gcaccggcgg ccagaaaacc agaaacaacg
     4201 cggcgctcaa gaccgccgtc gtccacaaag gcgattccag cgcggcgggc cagcggcgca
     4261 ggggtccatc cgccaagaag tcctcggcgc ggaacgcccc cgcgatggcc ccccccaagt
     4321 gcaagaggct cacccaatgg ggcaggttca ttcgccaaaa aatgggccat ccgcgggcga
     4381 aatgctttgg ggtcaatcga tcgaataggg ttcccatggt gtcgacctta agcatacccc
     4441 cgattgactg taattcaaga gtctaaaatc gcgcgatgtg gtaaaatgat ctcatccccc
     4501 tttgaaggag tcgttcatgc ggtttctcgt caccggcgcc ggcggattga tcggcgccaa
     4561 cgtgtccctc actttggccc aacggggcca cgacgtccac gccctggatc actttgccgt
     4621 tgggcggcgg gagaatttgg ccgattttaa aggcaccgtc cacaccgggg acattcgaga
     4681 tttcgatttc gccgcgctgg ggcggtttga cggggtgttc caccaggcgg ccatcaccga
     4741 caccaccgtg atggatgaaa agctgatgcg ggacgtcaac gtgcacgcct tcgttcgaat
     4801 tttggaggcc gcccatcgca ccggcacccc caaggtcgt
//
LOCUS       DAOQLS010000074         4829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8245_length_4829_cov_4.771261, whole genome shotgun sequence.
ACCESSION   DAOQLS010000074 DAOQLS010000000
VERSION     DAOQLS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8245_length_4829_cov_4.771261"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..720)
                     /locus_tag="PK362_02195"
     CDS             complement(172..720)
                     /locus_tag="PK362_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73685.1"
                     /translation="MKRMKGLLMAGAVVVMAGAAMAATTDTVVLTVTPTGTKSLSIDR
                     VSYDFGALNLGTTGNISSSSTVTNDGTLAATWAMRVSVDDAVWVASTTVGADRYKLMA
                     LLNAAAPVAANFNAGGGFDDVVVSAGAGLGAVSTATNYSGTQSGVNVLAGSTRGLWFN
                     LDMPTSSSVSTQRSFTVELEAQ"
     gene            1068..2381
                     /locus_tag="PK362_02200"
     CDS             1068..2381
                     /locus_tag="PK362_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNC73686.1"
                     /translation="MRLSRLVFLALVAVPGVTGFGMEPAAPPPVLTMAVTGPVDPAVK
                     NHLLDGFRRAEALNAGAVILRLDTPGGLLDATRDIVQAMINAPCPVIVHVAPRGARAA
                     SAGVFLTMAADVAVLAPETHLGAAHPVNLGGGAPGSEAPASSTGSANILGEKAVSDAA
                     AYIRGLAVAHGRNAVWAEDAVRRSVSLTAEEALANHVVDLIAADETELMDRLEGREVR
                     KNGRTFTLRFRGAARIDHPMSAAQRGLHVLGHPNIAYLLLVLGFYALVYEFATPGVGL
                     GGIAGITCLVLAFFSLQILPINTAGLVLLIAGLAMMAIDLIVSSHGLLIFGGLLAFGL
                     GSFLLFDGDSPAGRVSWPLIVGTLAGSAAYFGLALRKVFQARRAPPRTGAESLRGQTA
                     EVRPGGLVFVQGALWTADGLEGFKPGDRAIVLEVLGTRLRVGRPS"
     gene            2424..3191
                     /locus_tag="PK362_02205"
     CDS             2424..3191
                     /locus_tag="PK362_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNC73687.1"
                     /translation="MFGFGIGFLPIAIIAFIVIVNAVRVVPEYERGVIFRLGRVIGAK
                     GPGLFFLWPVIDRMVKVGLRTVTLDVPVQEIITKDNVPCQVNAVCYFKVIDPVRAVIE
                     IQDYFLATSQIAQTTLRSVLGQVEFDELLSHREKINTQLQQIIDTQTDPWGIKVSIVE
                     VKDVQVPEQMQRAMAHQAEAERDRRAKIIAAEGEFQASVKLAEAAKIIAAEPVTLQLR
                     YLQTATEIGRDKNTTILFPLPMEFINVLSNLVNKNKN"
     gene            complement(3231..4472)
                     /gene="thiI"
                     /locus_tag="PK362_02210"
     CDS             complement(3231..4472)
                     /gene="thiI"
                     /locus_tag="PK362_02210"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNC73688.1"
                     /translation="MNVVVVHYAELGLKGGNRRMFERKLMDDIRARLVPVHAHRVRLE
                     SARVLVELTENHVPEGVRAELGKVFGVAWFAFGRLLPWAPGNPGWEALAEGAVALVRS
                     FPQAKTFKVFARRAVKHYPLSSQDICVRLGKKIGEDTGLSVDLDHHDVGVHVEILTKE
                     ICFFAERTQGLRGLPKGSSGETLCLFSGGIDSPVAAWLMMRRGARVHYLHFHPFRSAA
                     DVRDSKIFELDRVLRATSPSSKLYLVPHDRYQVAASLAVPVEYETVFFRRFMFRAAES
                     LARREGYKALITGDSLGQVASQTLENLKAVQTELTLPVFQPVIAYDKESIVQLAQQIG
                     TYEPSIRAYKDCCSLMAKKPKTNVATPVVRRLEEQLDMPRLIEESLAQAELWDGATLR
                     PWTRGAYKEKTGGEIPPVFPA"
     gene            4564..>4829
                     /locus_tag="PK362_02215"
     CDS             4564..>4829
                     /locus_tag="PK362_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73689.1"
                     /translation="MYSLLAAFFRKFDGPAGHRRFSAWRTGIVLFWGGAAASAVHALP
                     YNPVQIEVEPVTAADRLTQGGVAWGDMDGDGNLDLVMSGQTAAGT"
BASE COUNT          861 a         1555 c         1469 g          944 t
ORIGIN      
        1 ccatcaccac caccgctccc gccatcagga gccctttcat ccgtttcatc atgtgtgtcc
       61 tcccgcgttc atcgacgcat cgcctttccg ccgaaccggg tcggcggggc attcccccgc
      121 cgacccgtcg atcaaacccc ggaaaggctc atccaaaccg taccgctggt gttattgggc
      181 ctccaattcg accgtgaagg accgctgggt gctgacgctc gaggacgtcg gcatgtccag
      241 gttgaaccac aggccccgcg tcgaacccgc caggacgttc acgccgcttt gcgtgcccga
      301 gtagttggtc gccgtcgaca ccgcgcccaa ccccgcgccc gccgacacca ccacgtcgtc
      361 gaacccgccg cccgcgttga agttcgccgc caccggcgcc gccgcgttca acaacgccat
      421 cagtttgtag cggtccgctc ccaccgtcgt gctcgccacc cacaccgcgt cgtccacgct
      481 cacccgcatc gcccacgtcg ccgccagcgt cccgtcgttc gtcaccgtgg aggacgacga
      541 gatgttgccc gtcgtgccca ggttcagagc gccgaagtcg tagctcaccc ggtcgatcga
      601 caggctcttc gtccccgtcg gcgtcacggt caacaccacc gtgtccgtcg tcgccgccat
      661 cgccgctccc gccatcacca ccaccgctcc cgccatcagg agccctttca tccgtttcat
      721 catgtgaacc ctccttgggt ttgatgcgac tcgaattgca ctcgttgcgt catcgtgttg
      781 aacaaatcgg gacacgcttt ttggggattg aataatatcg agaaaagggg cgaaaaccga
      841 tcgggcgatc gaggggaagg tgcgcaagaa gctcatcagg gcctccgagg ttacggtctt
      901 ggtgtatagc tataagcctg atctaagtca tgtatagccc ctgcttagct tatttgcaag
      961 ggatcttggc atggaatttg cctttcggcc aaaggtaaaa aacgcaaaaa aaggcacttt
     1021 tgggccttcc atgcgccgaa tcggcgggga actggtaaaa tgggcccatg cgtctctccc
     1081 gtctggtttt tttggccctc gttgcggtcc ccggggtgac cggctttggg atggaaccgg
     1141 cggctcctcc gccggtcctc accatggccg taaccggtcc cgtggacccg gccgttaaaa
     1201 accacctcct ggatggcttc cgacgggccg aggccctgaa cgccggcgcc gtgatccttc
     1261 gtttggatac ccccggcggt ctcctggacg cgacccggga catcgtccag gccatgatca
     1321 acgcgccctg cccggtcatc gttcacgtgg ccccccgggg ggcccgggcg gcttcggccg
     1381 gggttttttt gaccatggcg gcggacgtgg ccgtcctggc gcccgaaacc catttgggcg
     1441 cggcccatcc ggtcaatctc gggggcggcg cccccggttc cgaagccccg gcctcctcca
     1501 cgggatcggc caacattctc ggcgaaaaag cggtttccga cgccgccgcc tacattcgcg
     1561 gcttggcggt cgcccacggc cgaaacgccg tctgggcgga ggacgccgtt cgacgaagcg
     1621 tgtccctgac ggcggaggag gctctcgcca accacgtggt ggacctgatt gccgcggacg
     1681 aaacggaact gatggaccgg ctggagggac gggaggtccg gaaaaacggc cggacgttca
     1741 ccctgcgatt tcgcggcgcg gcccggatcg accaccccat gagcgccgcc caacggggcc
     1801 tgcacgtact cggccatccg aacatcgcct accttctcct cgttctcggt ttttacgccc
     1861 tggtgtacga gttcgccacg ccgggcgtcg gactgggcgg catcgccgga atcacctgcc
     1921 tggtcctcgc ctttttttcc cttcaaattc ttccgatcaa caccgcgggc ctcgtgctgt
     1981 tgatcgcggg attggccatg atggccatcg acctcatcgt ttcctcccac gggcttttga
     2041 ttttcggcgg gcttctggcc ttcggcctgg gctccttcct tctcttcgac ggggactccc
     2101 ccgccggccg cgtgtcctgg cccctcatcg tcggcacctt ggccgggtcg gcggcctatt
     2161 tcggtttggc gttgcgcaag gtttttcagg cgcgccgggc ccctccccga accggggcgg
     2221 aaagcctgcg gggacaaacg gccgaagtgc ggcccggggg cctcgtgttt gtgcagggcg
     2281 ccctgtggac ggccgacggg ctcgaggggt ttaagcccgg cgaccgggcg atcgttttgg
     2341 aagtgctggg gacccgcctt cgggtgggtc ggccgtctta gacggcggcc ccggacgttc
     2401 gatcaacgag aggagaacta aacatgtttg gattcggcat cgggtttttg cccatcgcga
     2461 tcatcgcttt catcgtcatc gtgaacgcgg tgcgcgtcgt accggaatat gaacgcgggg
     2521 ttatttttcg actggggcgc gtgatcggcg cgaaggggcc ggggctcttt ttcctctggc
     2581 cggtgattga ccggatggtc aaggtgggtt tgcggaccgt gacgttggac gtgcccgtcc
     2641 aagaaatcat caccaaggac aacgttccct gccaggtcaa cgcggtgtgt tatttcaaag
     2701 tcatcgaccc cgtgcgcgcg gtgatcgaaa ttcaggatta ttttttggcc acatcccaga
     2761 tcgcgcagac caccctgcgc agcgtgctgg gacaggtgga attcgatgaa ctgctctccc
     2821 atcgggaaaa gatcaacacc cagctccaac agatcatcga cacccagacg gacccctggg
     2881 gcatcaaggt gtccatcgtc gaggtcaagg acgtccaagt gcccgaacag atgcagcgcg
     2941 ccatggccca ccaggccgaa gcggagcggg atcgccgagc caaaatcatc gcggcggaag
     3001 gcgagttcca ggcgtcggtg aaactggccg aggcggcgaa aatcattgcc gccgaacccg
     3061 tgacgttgca actccgctat ctgcagacgg ccacggagat cggacgggac aagaacacga
     3121 ccatcctgtt ccccttgccc atggaattca tcaacgttct gtcgaacctg gtgaacaaaa
     3181 acaagaatta agcggtcgaa gcgggcggcg ccgccgcggg cggaccggat ttaggcggga
     3241 aaaaccggcg gaatttcccc gccggttttt tctttgtagg ccccccgggt ccagggacgg
     3301 agcgttgcgc cgtcccacag ttcggcctgg gccagggatt cctcgatcaa tcggggcatg
     3361 tccaattgct cctccagccg tcgcacgacg ggggtggcca cgttggtttt gggtttcttg
     3421 gccatgaggg aacaacagtc tttgtaggcg cggatggagg gctcgtaggt gccgatttgc
     3481 tgggccaatt gaacaatgga ttctttgtcg taggcgatga ccggttggaa aaccggcaac
     3541 gtcaactcgg tttgcacggc ttttaagttt tccaacgtct ggctcgccac ctggcccaga
     3601 ctgtcccccg tgatcaaggc tttgtagcct tcccgacggg cgagggactc cgcggcgcga
     3661 aacatgaagc gccggaaaaa caccgtttcg tattccaccg gaaccgccag ggaggccgcc
     3721 acctggtagc ggtcgtgcgg aacgaggtaa agtttggacg aaggactcgt ggcgcgcaac
     3781 acccggtcca attcgaatat tttcgaatcc cgcacgtccg cggccgatcg aaacggatga
     3841 aaatgaaggt agtggacccg ggcgccccgc cgcatcatga gccaggccgc cacgggcgaa
     3901 tcgattcccc ccgagaacaa acacagggtt tcccccgagg accccttggg caacccgcgc
     3961 aatccctggg tccgttccgc gaaaaagcaa atttccttgg tcaggatttc cacgtgaacg
     4021 ccgacgtcgt ggtgatcgag gtcgacggac aggccggtgt cttcgccgat tttttttccc
     4081 aaccgcacgc aaatgtcctg ggaggacaac gggtaatgct tgaccgcccg tcgggcgaac
     4141 actttgaagg ttttggcctg ggggaaggat cggacgaggg ccaccgcgcc ttcggccagc
     4201 gcctcccaac ccgggttccc cggggcccag ggcaacaacc ggccgaaggc gaaccaagcc
     4261 acgccgaaca ctttccccag ttccgcgcgg acgccctcgg gaacgtgatt ttccgttaat
     4321 tccacgagca cccgcgccga ttccaatcgg acgcggtggg cgtggacggg gacgagccgc
     4381 gcgcgaatgt cgtccatcaa ctttcgctcg aacatccggc gattgccgcc cttcagaccc
     4441 agttccgcgt agtgcacgac gacgacgttc atggaccgca tgttatcaaa aacgtcggcg
     4501 gacgcacgat gtccccgcca cttgcaaatt tcgtgccagg gggtatgctt taggcaataa
     4561 gctatgtact ccttattggc cgcatttttc cggaagttcg acggtcccgc gggtcaccgc
     4621 cggttttccg cgtggcggac ggggatcgtt cttttttggg ggggcgccgc ggcctccgcc
     4681 gttcatgccc ttccctacaa ccccgtccag attgaagtgg agcccgtgac ggcggccgac
     4741 cgtctgaccc agggcggggt ggcttggggc gacatggacg gtgatgggaa tttggatttg
     4801 gtgatgagcg gtcaaaccgc cgcgggaac
//
LOCUS       DAOQLS010000075         4774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8390_length_4774_cov_4.643357, whole genome shotgun sequence.
ACCESSION   DAOQLS010000075 DAOQLS010000000
VERSION     DAOQLS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8390_length_4774_cov_4.643357"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PK362_02220"
     CDS             <1..581
                     /locus_tag="PK362_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HNC73690.1"
                     /translation="AYISVLVALPGTPLFDRLRREGRLLPGEETRHTTHSAHSNIRFK
                     MAPGPFLRGYRDLVAQAFTPRAYFDRALRTLQRMSESRRLGERFESLRADFRSITRAA
                     ASGPRPKTSFLAPVTALWRFFAALPPSFRWQSIVFALRVACSVPDRWPWIFHFIAMGY
                     HYHRFTYENMLPGLDEAIAEADRRPNPLAAAV"
     gene            938..1011
                     /locus_tag="PK362_02225"
     tRNA            938..1011
                     /locus_tag="PK362_02225"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:972..974,aa:Glu,seq:ctc)
     gene            1367..2527
                     /gene="ugpC"
                     /locus_tag="PK362_02230"
     CDS             1367..2527
                     /gene="ugpC"
                     /locus_tag="PK362_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNC73691.1"
                     /translation="MARVILKNLTKSFGDHPIVNNVSLDIPDKEFFILVGPSGCGKST
                     TLRMIAGLEEMTSGEIHINGRMVNHVRPKDRDIAMVFQNYALYPHMSVYENMAFGLKL
                     RGYSRGEVDRRVREAADILTLGAYLDRKPRALSGGQRQRVAVGRAIVRKPQVFLFDEP
                     LSNLDAKLRVQMRLELSKLHERLQTTIVYVTHDQVEAMTMGDRICVMKDGEVQQVAPP
                     LELYQPPANKFVAGFIGNPPMNFFTVLVEAGRGGPVLRHASFTLTVPKTLADQVRPLI
                     GREVDFGIRPEDLYDRLFYNYPVSDGSSVKAVVDVVEPMGSEIYLYLKAGTVDLVVRV
                     PAYVKAEAGKPMDMVFNLERMHLFDRATGATLLKPIPAGPVPAPRPATVPAP"
     gene            2534..2914
                     /locus_tag="PK362_02235"
     CDS             2534..2914
                     /locus_tag="PK362_02235"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNC73692.1"
                     /translation="MYYKLLADGIVVLHALNVAYVLLGPIWAWRVSWFRFVHLISLWW
                     TFFVFAGGFYCPMTNFENSLRYHYNPAATYSTGFIIRYIGPMLWWDLTKPQVVGAMTA
                     WTAFWTLAYAWLWARKKPAPRAGG"
     gene            2911..3489
                     /locus_tag="PK362_02240"
     CDS             2911..3489
                     /locus_tag="PK362_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNC73693.1"
                     /translation="MNPRARAVGLALALAAPLRAGDAFGHRPYREAPAASLADAGDYR
                     SSGRTVRLTKDAAAAWKSMVAAAARDGVQLIPISGFRPVSYQRGLFKRATKKYGSPRD
                     AARWVAPPGHSEHHTGLALDLGDGARPDTDVDQRFESTPAHRWLTQHAREHGFELSFP
                     KDNPQGVSHEPWHWRWIGSAEGKRLFHPADRP"
     gene            3546..>4774
                     /locus_tag="PK362_02245"
     CDS             3546..>4774
                     /locus_tag="PK362_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC73694.1"
                     /translation="MAGEYIFTIEDLSKSFGPKKLFEGIYLSFYHKAKIGIVGENGSG
                     KSTLLRIMAGVEKDFRGKAEAKKGAKIGYLPQEPRLDPTKTVREEVEEAFADIKKMLA
                     DYDKISESMSGELSPDDMEKALEKMGRLQEQIEAVDGWNLKRQVDVAMDALLLPEDTT
                     LCNVLSGGEARRVALCKLLLLKPDMLLLDEPTNHLDAETVAWLENALKEYPGNVIVVT
                     HDRYFLDNITQWILELDHGKGIPFEGSYHEWLAAKAKRLADEDKRASAMQKHLEKELE
                     WMRQTPSGRRTKSKARVRDYEALASQQKTVDVNSLDIEVVPGPKLGNRVVDFENVSKA
                     YGDNGLMKDVSFSVPRGAIVGLVGPNGVGKTTLFRMLVGQEKPDAGQVKIGETVVFSY
                     VDQKREDLSPDKTVFQE"
BASE COUNT          903 a         1604 c         1355 g          912 t
ORIGIN      
        1 acgcttacat cagcgtcctg gtcgccctgc ccggaacccc gcttttcgac cgacttcgcc
       61 gggaaggccg ccttctcccg ggagaggaaa cgcgccacac gacccattcg gcccattcca
      121 acattcgttt taaaatggct cccgggccct ttttgcgggg ttatcgggat ttggtggccc
      181 aggcctttac gccccgggcc tacttcgacc gggcgctccg aacccttcag cgcatgtcgg
      241 aatcccggcg cctgggggaa cgattcgaat ctcttcgagc cgactttcga tccatcaccc
      301 gagccgccgc ttcggggccc cggccgaaaa cctcctttct ggctccggtc acggcgctct
      361 ggcgcttttt tgcggccctg ccgccttctt ttcgttggca atcaatcgtt tttgcgcttc
      421 gcgtggcctg ttctgtcccg gatcgttggc cctggatttt ccattttatc gccatggggt
      481 accactacca tcgcttcacc tacgaaaaca tgcttcccgg cctggacgag gccattgccg
      541 aggccgaccg ccgtccgaac cccctggccg cggccgtttg acagcgggaa cgccactcga
      601 tataatcaaa agacaggtct acttttcgtc cggacaatcc gttttactcc cacggcccct
      661 ctcggcagat cgagagggtt tgtcgtttta aaccggtctt ttccctgtca agtcggaatt
      721 ccatcgttgc gtcggcaaaa tggccctttc gtccgacggg gttgcggtcc ctccggggcc
      781 gccgtcggcc ccaaaagctc tgcgttgggg cgtgcggccc aggacgccgc cctcggtgcc
      841 ccccgggcac ggcggagagg atgtccccac gatttggcga gcgacaacgt cgctcgcttc
      901 cgctcaaggc ggagttccat cgttgcgtcg gcaaaatggc ccgttcgtct agcggcctag
      961 gacgccgccc tctcaaggcg gagatcacgg gttcgaatcc cgtacgggcc attttgccga
     1021 tgcctttgtc agcgggttcg cggtcggagc ggcggacctt gtccgacgcc tgaacagggc
     1081 ggagtcctcc cgatcaaggc cgggggccgt gatcccgtac gggccatttt gccgatgctt
     1141 ttatcagcgg gttcgcggtc ggagcggcgg accttgtccg acgcttgaac ggggcggagt
     1201 cctcccgatc aaggccgggg gccgtgatcc cgtacgggcc attttgccgg tgcttttgtc
     1261 accggtcttt ggcggcgcgc cgtcgcccct gtttccgacg atcggaaagt tgatacaata
     1321 attcttcgcc cgcgcggttt gcggagacca ggagacggga caccccatgg cgcgcgtgat
     1381 tctcaagaac ctgacgaaaa gcttcggcga tcacccgatc gtcaacaacg tttccttgga
     1441 catccccgac aaggaatttt tcatcctggt cggcccctcc ggttgcggca aatccaccac
     1501 ccttcgcatg atcgccggtt tggaggaaat gacttccggc gaaattcaca tcaacgggcg
     1561 gatggtcaac cacgtccgcc ccaaagaccg ggacatcgcc atggtgttcc aaaattacgc
     1621 cctctacccc cacatgtcgg tctacgaaaa catggccttt ggattgaaac tccgcggtta
     1681 ttcccgggga gaagtcgacc gccgcgtccg ggaagccgcc gacatattaa ccctgggcgc
     1741 gtatctcgac cggaaacccc gggccctctc cggcggccaa cgccagcggg ttgcggtggg
     1801 ccgcgcgatt gttcgcaaac cccaggtgtt cctctttgac gagcccctgt ccaatctgga
     1861 cgccaaactc cgggttcaaa tgcggctgga actctccaag cttcacgaac gtcttcaaac
     1921 gaccattgtg tacgtcaccc acgatcaggt ggaagccatg accatgggcg accgcatctg
     1981 cgtcatgaag gacggcgagg tccaacaggt ggcgcccccc ctggagcttt accagccccc
     2041 cgccaataaa tttgtcgcgg ggttcatcgg caacccgccc atgaattttt tcaccgtcct
     2101 ggtggaggcc ggccgggggg gaccggtgct tcgccatgcc tcctttacct tgaccgtgcc
     2161 gaaaaccctg gccgaccagg ttcgccccct gatcggacgg gaggtggact tcggcattcg
     2221 gcccgaagac ttgtacgacc ggcttttcta caactacccc gtcagcgacg ggtccagcgt
     2281 caaagcggtg gtggacgtcg tggagcccat ggggtccgag atttatctgt acctcaaggc
     2341 cggcacggtg gacttggtcg tgcgcgttcc cgcctacgtg aaagccgagg ccgggaaacc
     2401 catggacatg gttttcaatt tggagcgcat gcatctcttt gatcgggcga cgggcgcgac
     2461 cctgctcaaa ccgattcccg ccggcccggt ccccgcgccc cggcccgcca ccgtcccggc
     2521 cccctaaccg tccgtgtatt acaaactcct cgccgacggc attgtggtgc ttcacgccct
     2581 caatgtcgcc tacgttctgc tcggccccat ttgggcctgg cgcgtctcct ggttccgctt
     2641 cgtccaccta atcagcctgt ggtggacgtt cttcgtcttc gccggcgggt tctattgccc
     2701 catgaccaat ttcgaaaaca gcctccggta tcactacaat cccgccgcga cgtacagcac
     2761 cggattcatc atccgctaca tcggtcccat gctctggtgg gacctcacca agccccaggt
     2821 cgtcggcgcc atgacggcct ggaccgcctt ttggaccctg gcgtacgcct ggctttgggc
     2881 ccggaagaaa cccgcccccc gcgccggcgg atgaacccgc gagcccgcgc cgtcggtctg
     2941 gccttggcct tggccgctcc ccttcgggcg ggcgacgcct tcggccatcg cccctaccgg
     3001 gaagcgccgg ccgcttcgct ggccgacgcg ggagactacc gctcctcggg acgaacggtg
     3061 cgattgacga aggacgccgc ggccgcctgg aaatccatgg ttgccgccgc cgcccgggac
     3121 ggcgttcaat tgattcccat atcgggtttc cgacccgtgt cctaccaacg gggtcttttt
     3181 aagcgcgcca caaaaaaata cggctcgccc cgggacgcgg cgcgctgggt cgccccgccg
     3241 ggccattcgg aacaccacac cggcctcgcc ctggatctcg gcgacggcgc ccggcccgac
     3301 accgacgtcg atcaacgttt tgaatccacg cccgcccacc gttggctgac ccagcacgcc
     3361 cgggaacacg ggtttgaact gtcctttccc aaggacaatc cccagggcgt cagccacgaa
     3421 ccctggcatt ggcggtggat cggctccgcc gaaggaaaac gccttttcca ccccgcggac
     3481 cgtccgtaac cccgcggttc ccacaaggta taattggttt ttgattccca ctaaaagagg
     3541 ttcccatggc cggcgaatat atttttacga ttgaagatct ctccaaatcc ttcgggccga
     3601 agaagttgtt cgaaggcatt tatctctcct tttaccacaa agccaaaatc ggcatcgtgg
     3661 gcgaaaacgg ctccggcaaa tccaccctgc tccgcatcat ggccggcgtg gaaaaagatt
     3721 tccgcggcaa agccgaagcc aaaaaaggcg ctaaaatcgg ctacctcccc caggaacccc
     3781 gcctcgaccc gacgaaaacc gtccgggagg aggtggagga agccttcgcc gacatcaaga
     3841 aaatgctggc cgattacgac aaaatttccg aaagcatgtc cggggagctt tccccggacg
     3901 acatggaaaa ggccctggaa aaaatgggcc ggttgcagga gcaaatcgaa gccgtggacg
     3961 ggtggaacct gaaacgccag gtggacgtgg ccatggacgc cctgctcctg cccgaggaca
     4021 ccacgctctg caacgtgctc tccggcggcg aggcccgccg ggtggccctc tgcaagctgc
     4081 tgttgctcaa acccgacatg ctcctcctgg acgagcccac caaccacctg gacgccgaaa
     4141 cggtggcctg gctggaaaac gccctcaagg agtaccccgg caacgtcatc gtcgtcaccc
     4201 acgaccgtta tttcctggat aacatcaccc agtggatcct ggaattggac cacggcaaag
     4261 gcattccctt cgagggcagc taccacgaat ggttggcggc caaggccaaa cggctggccg
     4321 acgaagacaa gcgggcctcg gccatgcaga aacacttgga aaaggaattg gaatggatgc
     4381 gccagacccc ctcgggccgc cgaaccaaaa gcaaagcccg cgtccgcgac tacgaagccc
     4441 tggcctccca acagaaaacc gtcgacgtca acagcctgga catcgaagtc gttccggggc
     4501 ccaagctcgg aaaccgggtc gtggattttg aaaacgtctc caaggcgtac ggcgacaacg
     4561 gcctgatgaa agacgtgagt ttcagcgttc cccggggcgc catcgtgggc ctcgtgggcc
     4621 cgaacggcgt cgggaaaacc acgctcttcc gcatgctggt cggccaggaa aaacccgacg
     4681 ccgggcaagt caagatcggc gaaacggtcg tgttctccta cgtcgaccag aaacgcgaag
     4741 acctctcccc ggacaaaacc gtcttccagg aaat
//
LOCUS       DAOQLS010000076         4756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8438_length_4756_cov_3.922782, whole genome shotgun sequence.
ACCESSION   DAOQLS010000076 DAOQLS010000000
VERSION     DAOQLS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8438_length_4756_cov_3.922782"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1342
                     /gene="murD"
                     /locus_tag="PK362_02250"
     CDS             8..1342
                     /gene="murD"
                     /locus_tag="PK362_02250"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNC73695.1"
                     /translation="MEGRRALVVGLGKSGAAAATLLSRSGARVAVTESRPSEAVAEWI
                     RRLPRAAARETGRHRFLEEKWDLIVPSPGVPSRVWADAARRGARVWGELELGYRALCA
                     AGRWPDRTAAITGTNGKTTTTALLGAMARADGRPTLVGGNIGTPLCDLVDRVTPETAL
                     ILEVSSYQLETAAAFRPAVGAVLNVTPDHLGRHGTMENYARTKFRLFQNQGAGDAAVL
                     NDRDPWCRRLAPLPPGRVTWFGRRSPGVGWDGRRLRSSLPGAGGDWSAPSHLPGRHNI
                     ENAEAAVACARLLGLSAPAVARALRNFPGVEHRLESVRLWRGVAFVNDSKATNVDSTR
                     VALKAFPGPVRLLLGGEHKGSPYTPLRGLLRGRVAEILLIGEAAPIIARDLAGTVPLA
                     RCGTLAAAVQRVAATARRGDTVLLSPACASFDQFLNYEDRGRRFKNLVAALP"
     gene            1339..2469
                     /gene="ftsW"
                     /locus_tag="PK362_02255"
     CDS             1339..2469
                     /gene="ftsW"
                     /locus_tag="PK362_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNC73696.1"
                     /translation="MTRAAVPASPDRVVFGLTIVLAVLGWMILYSASALVADGRFGDQ
                     YYFLKRQMIWTVLGLVGLTVACRVPLARVQKWSRPLLLGVVVLLVLVLAVGHEVGGAK
                     RWLRLGGLGLQPSEFAKLAVVLALADYLDRKQSRLGNWAGGLLPALILTGAVLGLVAL
                     ERDLGTPLLIGSVAIGMIYLAGARLAHLAALGLGLLPVVGTAVLAVPYRRQRLFAFLD
                     PWHDAQGAGYQLVQSLLALGSGGFWGKGSGESTIKMYYMPESQTDFIFPIFGEEFGFW
                     GTSLLVAIFFVLTYRCFQIAREATHSFHALTAAGVGLLLGGQVLINLGVVTGLLPTKG
                     MPLPFVSFGGSSLLVMFTAIGLVLNVSRRNAGGPSAPFRGRA"
     gene            2471..3574
                     /locus_tag="PK362_02260"
     CDS             2471..3574
                     /locus_tag="PK362_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNC73697.1"
                     /translation="MRVCLAAGGTGGHLLPGLALGKALRERGHLVHFIVKIDETSQDR
                     VAREGFPSSAFFFEGFPRKLSLRALAFPWIAAAAYRSAARIARRETPRVIVGMGGYVS
                     VPLGLWAAGHRVPLVLHEQNVRAGLANRFLSRWAAAIGVTFEGTTGLPAERCTTTGLP
                     LRPDLRPGDPRAARRALGLAENDGTILVFGGSQGARALNARWIDSLSRLRRPGENWQF
                     IHLTGPQDEAAVRAAYERDGRRAFVRAFHSDMATVYSAADVVVGRAGANTVMEIYRMG
                     KAALLVPFPHATSDHQTANALALARGGAATVVAEGELTVDRLADLVNAWPALDDLRRA
                     NDARLAAVPADFSRAAERLADLVESAVGKELPA"
     gene            3571..4368
                     /gene="surE"
                     /locus_tag="PK362_02265"
     CDS             3571..4368
                     /gene="surE"
                     /locus_tag="PK362_02265"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC73698.1"
                     /translation="MNRKTILVVNDDGIHGPGLPPLVNALRPLGRVVVVVPQQERSAA
                     SHAITLHKPIRLREIKKDWYITNGTPADCVRLGVTGILRERLDAVVSGVNQGANLGSD
                     TLYSGTVGAAKEGCLLGFPSVAFSLTSKDGARFDTAARFARSLVRWMLGNALPPHCFF
                     NVNVPDVAWTELRGAAMTRLGRRLYGRRLTRRRDPRGQHYYWMAGAIPRGVAEVDSDV
                     GAVARRVISVSLLRLLATVEDGPSTRGPWPFLFSAPGAGRPNSKRAR"
     gene            complement(4346..>4756)
                     /locus_tag="PK362_02270"
     CDS             complement(4346..>4756)
                     /locus_tag="PK362_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73699.1"
                     /translation="PALSATAGNLRVNGSLVYANGTNAASAQDSFAALASNNIQIDCT
                     IDPPPQAPNNTHFTILNGVFSSVNSGQVNMVNATGGGFAGLKFYGTRTNLFTLTAGGG
                     TWTPSEFYYDANLVKYPPPGLPEKPKLVTWRASN"
BASE COUNT          641 a         1518 c         1660 g          937 t
ORIGIN      
        1 ccgttggttg gaggggcgcc gggctctggt cgtgggcctc ggaaaatccg gcgccgcggc
       61 ggcgaccctc ctgtcccgga gcggggcccg ggtggccgtg accgaaagcc gtccgtcgga
      121 ggccgtggcc gaatggatcc ggcggcttcc ccgcgccgcg gctcgggaaa cgggccgtca
      181 tcgtttcttg gaagaaaaat gggatttgat cgttccgagc ccgggggtcc cttcgcgcgt
      241 gtgggcggac gccgcccggc ggggcgcccg cgtgtggggc gaattggagc tcggctatcg
      301 cgccctttgc gccgcgggtc gttggcccga ccgcaccgcc gcgatcacgg gcaccaacgg
      361 gaagacgacg acgacggccc ttttgggcgc catggcccgg gcggacggac gccccacgtt
      421 ggtcgggggg aacatcggaa ccccgctctg tgatttggtc gaccgcgtga cgccggaaac
      481 cgctttgatt ttggaagtgt ccagttacca actggaaacc gccgcggcgt ttcgacccgc
      541 ggtcggcgcg gtgctgaacg tcacccccga tcacctggga cgccacggta ccatggaaaa
      601 ttacgcccgc accaaattcc gcctttttca aaaccaaggg gcgggggacg cggcggtgct
      661 gaacgaccgc gatccctggt gccggcgtct ggcgccgttg ccgcccggcc gggtgacctg
      721 gttcggccgc cgttcgccgg gcgtgggttg ggacgggcgg cgtcttcgct cctccttgcc
      781 cggggcgggg ggcgactggt cggcgccgtc ccacttgccc ggccgtcaca acatcgaaaa
      841 cgccgaggcg gccgtcgcct gcgcccgtct gctgggcctg tcggccccgg ccgtcgcccg
      901 cgcgctacgg aattttcccg gcgtggagca ccgattggag tccgttcgcc tttggcgcgg
      961 cgtcgccttc gtcaacgatt ccaaggccac caacgtcgat tccacccggg tggccctgaa
     1021 agcgttcccc ggtccggttc gactcctgct cgggggagaa cacaaaggct cgccctacac
     1081 cccgctgcgg ggactcctgc gggggcgggt ggcggaaatt cttctcatcg gggaggccgc
     1141 cccgatcatc gcccgggatt tggcgggaac ggttcccctg gcccgatgcg gcaccctggc
     1201 ggccgccgtt cagcgggtcg cggcgacggc ccgccggggg gacaccgtgt tgctttcgcc
     1261 cgcctgcgct tccttcgacc agtttttgaa ttatgaggac cgcggccggc gattcaagaa
     1321 tctagtggcg gcgctgccgt gacgcgcgcg gcggttcccg cctcccccga ccgggtcgtg
     1381 ttcggtttga ccatcgtcct ggccgttctc ggctggatga tcctctattc cgccagcgcc
     1441 ctggtggccg acgggcgctt cggcgaccag tactattttt taaagcgcca aatgatttgg
     1501 accgttctgg gtctggtcgg actgaccgtc gcttgccgcg ttcccctcgc ccgcgtccag
     1561 aaatggagcc gacctctcct cctgggcgtc gtggtcctgc tggtgttggt cctggccgtg
     1621 ggccacgagg tggggggcgc caagcgctgg ttgcggctcg gcgggttggg actccagccg
     1681 tcggagttcg ccaaactggc cgtggtgctg gccctcgccg attatttgga ccgcaaacaa
     1741 agccgcctcg gcaattgggc cgggggcctt ctgcccgccc tgatactcac cggcgcggtg
     1801 ttggggttgg tcgctttgga acgggatctt ggaacgccgc tcttgatcgg atcggtggcg
     1861 atcgggatga tttatttggc cggcgcgcgg ctcgcccatt tggcggccct gggattgggc
     1921 ctgctccccg tggtcggcac cgccgtgttg gcggtcccgt accgtcgaca acgtttgttc
     1981 gcctttttgg atccctggca cgacgcccag ggcgccggat atcaactggt ccaatcgctc
     2041 ttggccctgg ggtccggggg gttttggggg aaagggtccg gggaatccac gattaagatg
     2101 tattacatgc cggaatccca gacggacttc atttttccca ttttcgggga agaattcggt
     2161 ttttggggca cctccctcct ggtggccatt tttttcgttt tgacctaccg ttgtttccaa
     2221 atcgcccggg aggcgaccca ctcctttcac gcgttgacgg cggcgggggt ggggctgttg
     2281 ctcggggggc aggtgttgat caatttgggc gtcgtgacgg ggctgctccc caccaaagga
     2341 atgcccctgc ccttcgtgtc tttcggggga tcctccttgc tcgtcatgtt caccgccatc
     2401 ggcttggtgt tgaacgtttc tcgtcggaac gcggggggcc cctccgctcc tttccgggga
     2461 agggcctgac gtgcgggtgt gtttggcggc gggggggacg ggggggcatc ttcttcccgg
     2521 tctggccctt ggaaaggccc tgcgggaacg ggggcacctc gttcatttta tcgtcaagat
     2581 agacgaaacc tcccaggacc gtgtggcccg ggaagggttt ccctccagcg cgttcttttt
     2641 tgaagggttc ccccgaaaac tctcccttcg agcactcgcc ttcccctgga tcgccgcggc
     2701 cgcctatcga agcgcggccc gcatcgcgcg ccgggaaacg ccccgggtga tcgtcggcat
     2761 gggggggtac gtgtcggttc ccttgggcct ttgggcggcg ggccatcggg tgcccctggt
     2821 cttgcacgag caaaacgtcc gggccggact ggccaatcgc tttttgtcgc ggtgggccgc
     2881 ggccatcggc gtcacgtttg aggggaccac gggactgccc gcggaacgtt gcacgaccac
     2941 cggactgccc cttcgtccgg atttgaggcc gggcgatccc cgggccgctc gacgggccct
     3001 ggggttggcc gaaaacgacg gaacgatcct ggtcttcggg ggaagccaag gggcccgggc
     3061 cctcaacgcc cgttggatcg attccctgtc ccgccttcgc cggccggggg aaaattggca
     3121 attcatccat ttaacgggtc cccaggacga agcggcggtg cgcgccgcct acgaacggga
     3181 cggccggcgg gccttcgttc gggcgtttca ttcggacatg gccaccgtct attcggcggc
     3241 cgacgtcgtg gtggggcgcg cgggcgccaa caccgtcatg gaaatttacc ggatggggaa
     3301 ggccgcgctt ctggttccgt tcccgcacgc gaccagcgac caccagaccg ccaacgccct
     3361 ggccttggcc cgcggggggg ccgcgacggt cgtggccgaa ggggagttga ccgtcgatcg
     3421 gttggccgac ctggtgaacg cctggcccgc cctcgacgac ctccgtcggg ccaacgacgc
     3481 gcggttggcc gccgttccgg ccgatttttc ccgggcggcg gagcggttgg cggacctcgt
     3541 ggaatccgcc gtcggaaagg agctccccgc gtgaatcgaa aaacgatttt ggtcgtcaac
     3601 gacgatggca ttcacggccc cggcttgccg cccctggtga acgcccttcg tcccctgggg
     3661 cgcgttgtgg tcgtcgtgcc ccagcaggag cgttcggcgg cgagccacgc catcaccctg
     3721 cacaagccga tccgccttcg ggaaatcaaa aaggattggt acatcaccaa cggcactccg
     3781 gccgactgcg tgcggttggg cgtgaccggg attcttcggg aacgtttgga cgcggtggtg
     3841 tcgggggtca accagggggc caatctcggc tccgacaccc tctattccgg cacggtcggg
     3901 gcggcgaagg aagggtgttt gctgggattc ccgtcggtgg cgttttccct gacgtccaag
     3961 gacggcgcgc ggttcgacac ggccgcccgc ttcgcccgct ccctggtccg ctggatgctg
     4021 gggaacgcgc ttccgcccca ttgttttttc aacgtgaacg tcccggacgt ggcctggacg
     4081 gaacttcggg gcgccgccat gacccggttg ggccgccgcc tttacgggcg gcggctcacc
     4141 cgccgccggg atccccgggg ccagcattat tattggatgg cgggcgccat tccccggggt
     4201 gtggccgagg tggattcgga cgtgggcgcc gtggctcgac gcgtgatttc ggtgagtttg
     4261 cttcgtctcc tcgccaccgt ggaggacggg ccttcgacgc gcgggccctg gccctttttg
     4321 ttctcggcgc cgggcgccgg tcggcctaat tcgaagcgcg ccaggtgacg agtttcggtt
     4381 tttcgggaag gccgggcgga gggtatttga cgaggttggc gtcgtaatag aattccgagg
     4441 gcgtccaggt cccgccgccc gccgtcagcg tgaataaatt ggtgcgcgtt ccgtaaaatt
     4501 taagcccggc gaatccaccc cccgtggcgt tgaccatgtt gacctggccg ctgttgaccg
     4561 aggaaaacac cccgttgaga atcgtaaagt gggtgttgtt cggggcctgg ggcggaggat
     4621 cgatcgtgca gtcgatttga atgttgttgg aggccagcgc cgcaaaggaa tcctgggcgc
     4681 tggccgcgtt cgtcccgttg gcgtagacca acgagccgtt gacgcggagg ttgccggcgg
     4741 tcgccgacaa ggccgg
//
LOCUS       DAOQLS010000077         4741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8486_length_4741_cov_4.081733, whole genome shotgun sequence.
ACCESSION   DAOQLS010000077 DAOQLS010000000
VERSION     DAOQLS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8486_length_4741_cov_4.081733"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="PK362_02275"
     CDS             <1..975
                     /locus_tag="PK362_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC73700.1"
                     /translation="NCTDALNLALHGLPWKAGDRALVSPFEHNSVLRPLNALKARRGI
                     VVDFMPTDGDGRVRPEGLSRVLRARTRLIACLHASNVTGVLQPLAALGAFARRRGLPF
                     LVDAAQSAGAVPLDVRRMNIDYLAFPGHKALLGPLGTGGLIVAPGNDPAPARQGGTGS
                     WSETDVQPTEFPDRLESGSHNAPGLLGLLEGVRYVAARGVESIRRAERARIDQFREGV
                     RGIPGLRLFAPRSAEHQVAVFSFLFDGEDPQRSAARLWARHRLMVRAGLHCAPGAHRF
                     IGTLPRGTFRFSFGPWTSAGDVARAIEALRAPAPRRRQLSRVPGDKVQ"
     gene            1003..4467
                     /locus_tag="PK362_02280"
     CDS             1003..4467
                     /locus_tag="PK362_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73701.1"
                     /translation="MPNPAPNAESELYSYINLKLAALGQPAVDAVRSDNLLSVAQPLL
                     RNYREKDRLLSQYLCPADWRIQHFLDDYFQADPLTEPVPLPSTTFVLDRPGLARALSL
                     PPTSDRFVSNIVTSYRVAQGVLHNPKSDRRTTAGVFHIAEGGLPIPEDKKAVPKAVYR
                     NLLNEAFRPPEDLLELPFTADQKEKARLFVSLLLRPLVRPPVPGRRPAQSMEIRFFVP
                     GNLVANLDFVESIFDNGGDPYLPENDAAMDLEGWTGHTGCVILAPHLVRLTKKSLGLP
                     PLDKATERQRRDGMCWASPEELYNEGQAFKITARDERGVMVTVIADNYFGYCKKEVKT
                     QISYAANLYGGVEEEHSGGALAFPSYAEGDSFHQEERILPQNGQSFRDVAALYGDGMI
                     VRPEGYGVDKKYPDIYYVPSDARFDLVSKSVTWSLEGHTQNLKLLAGAAYVYPNGYKV
                     FLEKQPGGKGWHLVSAAPEGTLCHKPSTVSGGGKSEISKSILDAMVQGPVFVANFKED
                     FDQVEAVINGEFGHRFKNPKVMTRPSRPILSPRRSLGSVIKLLTPAEEYIDEFNKWLR
                     SIPPHILDLIFIVKRFYREEWGTRWRERFSVDAINGAPGHELKLDGKRLTAQYLRVGF
                     DPDGSRRILRVRQDFNAARKVQVEDDITASVVVAAKDLAGLAAGIGEGSVKLVQNCER
                     RLFQRPDDAIHPGYDKQAEADIAGPGVFLSNFEPLSRERAREMVEDAMGFDLYTEPIQ
                     DLFLGFLRSARPDYIVASSVPRMFDGRPSKNPRYLQNRPDLMDHRSTYLAELGARLFR
                     KIPPGQPCVSPVNAVLAGRRNNPPAPEIGAPPLAVYNPIHYQELPEFFMDLISSLTGK
                     SPSTTGFGSEGALTKGPFNALWPIIDLNNALVSFVLTGQAGFTTAAGHVGPRYKVDHD
                     ISLLVPEIWCRLSAEERCPREMIRQGQLEKVEDFDFEGRRIPASRLGYRITQKFARAF
                     LGRIFNNPNVVFPEDMLKPELQDRGVFVEGVEAIAAAHQRVALDYFADGSVEAACPPL
                     RALLHIMAHGHFEGKNVTDPAIRGLFTAESVLSSEWYRRRLETQAERDRALWSRHVAA
                     LEEASADPVRSETIGRAALRDKLNRARQELKKAGSSDYVDGLRGGLGADPFGVKTPEP
                     PRSAVAAR"
     gene            complement(4469..>4741)
                     /locus_tag="PK362_02285"
     CDS             complement(4469..>4741)
                     /locus_tag="PK362_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNC73702.1"
                     /translation="GPGVGWVLWGAAITLVPLLVVGIVARLILKTNYTALCGLLAGAM
                     TDPPALAFANAHFGSEAPALSYATVYPLTMLLRVLSTQAMVLLLMR"
BASE COUNT          822 a         1599 c         1498 g          822 t
ORIGIN      
        1 aactgcaccg acgccctgaa cctggccctt cacggcctgc cctggaaagc cggggatcgg
       61 gccctggtgt ctcccttcga acacaattcc gttctccggc ccctgaacgc cctcaaagcc
      121 cggcggggca tcgtcgtcga ttttatgccg acggacgggg acgggcgcgt gcgcccggaa
      181 ggtttgtccc gggtccttcg ggcccgcacc cgcttgatcg cctgtctcca cgcctccaac
      241 gtgacggggg tcctccaacc cctggcggct ctgggggcct ttgcccggcg gcggggcctc
      301 ccctttctgg tggacgccgc ccaatcggcg ggggcggtgc ctctggatgt gcgacggatg
      361 aacatcgatt atttggcctt tccgggacac aaagccctct tgggtccctt gggcaccggg
      421 gggctgatcg tcgccccggg caacgacccg gcgccggccc gccagggggg aacgggcagt
      481 tggtccgaaa cggacgttca accgacggaa tttccggacc gtctggagtc cggcagtcac
      541 aacgcccccg gtctgctggg tcttttggaa ggggtgcgat acgtggcggc ccggggcgtg
      601 gaatccattc gacgggccga gcgggcgcgc atcgatcaat ttcgggaagg cgtccgaggg
      661 atcccgggcc tccgcctctt cgcgccgcgg agcgccgagc atcaggtcgc cgtcttttcc
      721 tttctcttcg acggagaaga tccccaacgg tccgcggcgc ggctctgggc ccgccaccga
      781 ttgatggttc gggccggcct ccactgcgcc cccggcgccc accggtttat cggcaccctg
      841 ccccggggca ccttccgttt ttccttcggc ccctggacct cggccgggga cgtggcgcgc
      901 gccatcgaag cccttcgggc ccccgcgccc cgccggcgtc aactttccag ggttccgggc
      961 gataaagtac aatagaccca tcccatacga cggaggtcgt cggtgccgaa ccccgctccg
     1021 aacgcggaga gcgagcttta ctcctacatc aacctgaagc tggccgccct cgggcagccc
     1081 gccgtggacg ccgtgcgcag cgacaatctc ttgtcggtgg cccaacccct gctccgcaat
     1141 taccgggaga aggaccggct cctgtcccaa tacctctgcc cggccgattg gcggatccag
     1201 catttcctcg acgattattt tcaggccgat cctctgacgg aaccggtgcc ccttccctcg
     1261 acgaccttcg tcctcgaccg cccgggtttg gcccgggcgc tgtccctgcc gcccacctcc
     1321 gaccgttttg tttccaacat cgtgaccagc taccgggtgg cccagggcgt cctgcacaac
     1381 cccaaaagcg accgccggac caccgccggg gtttttcaca tcgccgaggg cggcctcccg
     1441 attcccgagg acaaaaaggc cgtgcccaag gcggtctacc ggaatttgct caacgaagcc
     1501 ttccgccccc ccgaggacct tttggagctg cccttcacgg cggaccaaaa ggaaaaagcg
     1561 cggctcttcg tttctctcct cctgcggccc ctcgtccggc ccccggtgcc cggccggcgt
     1621 cccgcccaaa gcatggaaat ccggtttttc gtcccgggca acctcgtcgc caatttggat
     1681 tttgtggagt cgattttcga caacggcggg gacccctacc tgcccgaaaa cgacgccgcc
     1741 atggacctgg agggatggac cggccacacg ggctgcgtga ttttggcgcc ccacctggtg
     1801 cggctcacca agaagtccct gggcctcccg cccctggaca aagccaccga acggcaacgg
     1861 cgggacggca tgtgctgggc gtcccccgag gagctttaca acgaggggca ggcctttaaa
     1921 atcaccgccc gggacgagcg gggcgtgatg gtcacggtca tcgccgacaa ctatttcggc
     1981 tactgcaaga aggaagtcaa gacccagatc agctacgccg cgaacctcta cggcggcgtc
     2041 gaagaggagc actcgggcgg cgccctggcg tttccgagct acgccgaggg ggacagtttc
     2101 caccaggaag agcgcatcct cccgcagaac ggccaaagtt ttcgcgacgt ggcggccctc
     2161 tacggcgacg gaatgatcgt gcggcccgaa ggctacgggg tcgataagaa ataccccgat
     2221 atttactacg ttccatcgga cgcgcggttt gatttggtgt ccaagtccgt gacctggtcc
     2281 ctggaaggcc acacgcaaaa tctcaagttg ttggccgggg cggcctacgt ctaccccaac
     2341 gggtacaaag tgtttttgga gaagcagccg gggggcaagg gttggcattt ggtgagcgcg
     2401 gcccccgaag gcaccctctg ccacaaaccg agcacggtgt ccggcggcgg aaaatccgaa
     2461 atttcgaagt ccatcctgga cgccatggtc caggggccgg tgttcgtcgc caacttcaaa
     2521 gaggattttg accaggtcga ggccgtgatc aacggcgagt tcggccaccg gttcaaaaat
     2581 cccaaagtca tgacgcggcc gtcgcggccc atcttgagcc cccgccggtc cctggggtcc
     2641 gtgatcaaat tgctgacccc ggccgaggaa tacatcgacg aattcaacaa atggctgcgg
     2701 tccatcccgc cgcacatcct ggatttgatt ttcatcgtca agcggtttta ccgggaggag
     2761 tgggggacgc gttggcggga acggttcagc gtcgacgcca tcaacggcgc ccccggccac
     2821 gaattgaaat tggacggcaa gcggctcacg gcccagtatc tccgcgtggg cttcgacccc
     2881 gacggctccc ggcgcatttt acgcgtccgg caggatttca acgccgcccg caaggtccag
     2941 gtggaggacg acatcaccgc ctcggtggtg gtggcggcca aggatttggc gggtctggcc
     3001 gccggaatcg gggaaggaag cgtcaagctg gtgcagaact gcgaacgccg gctgtttcag
     3061 cgccccgacg acgcgatcca tcccgggtac gacaagcagg cggaggccga catcgcgggt
     3121 cccggggtgt tcctgtccaa tttcgagccg ctgtcccggg aacgggcgcg ggaaatggtg
     3181 gaagacgcca tggggttcga tctctacacc gaacccattc aggacctgtt cctcgggttt
     3241 cttcgttcgg cccggccgga ttacatcgtg gcctcctcgg tgccgcggat gttcgatggc
     3301 cggccttcca agaacccccg gtatcttcaa aaccgccccg acttgatgga ccatcgctcg
     3361 acctatctgg ccgagctggg cgcgcgcctg tttcggaaaa ttcccccggg ccaaccctgc
     3421 gtctccccgg tgaacgccgt gttggccggg cggcgcaaca acccgcccgc ccccgagatc
     3481 ggcgcgccgc ccctggccgt gtacaatccg atccactacc aggaactccc cgaatttttc
     3541 atggatttga tttcgagtct cacgggcaaa tccccgtcca ccacggggtt cgggtccgag
     3601 ggcgctttga ccaaggggcc cttcaacgcc ctgtggccca tcatcgattt gaacaacgcc
     3661 ctcgtgtcct tcgtcttgac gggccaggcc ggtttcacga ccgcggcggg gcacgtcggc
     3721 ccccgctaca aggtggacca cgacatcagc ctgttggtgc cggagatctg gtgccggttg
     3781 tccgccgagg agcggtgccc ccgggaaatg atccgccagg gccaattgga aaaggtggag
     3841 gattttgatt tcgaggggcg gcgaatcccg gccagccggc tggggtaccg catcacccag
     3901 aaattcgccc gggcgtttct gggtcggatt ttcaacaacc ccaacgtcgt gtttcccgag
     3961 gacatgttga aaccggagct gcaggaccgc ggggtgttcg tggaaggggt ggaggccatc
     4021 gccgccgccc atcaacgagt cgccctggac tatttcgccg acggcagcgt cgaggcggcc
     4081 tgcccgccgc tgcgggcgct gttgcacatc atggcccacg ggcatttcga aggcaaaaac
     4141 gtgacggacc cggcgattcg cgggttgttc acggcggaaa gcgttttgtc gagcgaatgg
     4201 tatcgccgcc ggctggaaac ccaggcggag cgggaccgcg ccctctggtc ccgccacgtg
     4261 gcggcgctgg aagaggcctc cgccgatccc gttcggtccg agaccatcgg ccgggcggcc
     4321 ctccgggaca aattaaaccg ggcgcggcag gagctgaaga aagcgggatc gtccgactac
     4381 gttgacggac ttcgcggagg gttgggcgcc gatcccttcg gcgtcaagac cccggaaccg
     4441 ccccgttcgg cggtcgcggc ccgctgagtc agcgcatcag aagcaggacc atggcctggg
     4501 tcgagaggac ccggagcagc atggtgagcg gatacaccgt cgcgtaggac aaggccggcg
     4561 cctccgaccc gaagtgggcg ttggcgaagg ccagggcggg cgggtcggtc atggccccgg
     4621 ccagcaaccc gcagagcgcc gtgtagttcg ttttcagaat taagcgcgcc acgatcccca
     4681 ccaccaacag cggcaccagc gtgatcgccg ccccccaaag aacccaaccc acccccggac
     4741 c
//
LOCUS       DAOQLS010000078         4655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8738_length_4655_cov_3.607174, whole genome shotgun sequence.
ACCESSION   DAOQLS010000078 DAOQLS010000000
VERSION     DAOQLS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8738_length_4655_cov_3.607174"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK362_02290"
     CDS             <1..714
                     /locus_tag="PK362_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73703.1"
                     /translation="GLAGLSALGWMVVRSPRPRVNGVQRSLLLGGGGMILGVLFLQSV
                     HWGREDTKFSSLVFPVFLGLALGAAAVARVPARRNALRGALLAFWAGTFGCNLTAAIV
                     PGSRLENNTELMKALFVRDHTPANAVVVINGGMLRLYLPGNAARQAVDLAYPLALTSK
                     AGARAFVEDQIRTTVLAGRPLFAVNEVVDASAWAETSDRGWDRTDRDRWLAPFDVQET
                     ARRNDWALYALRPRTEVRR"
     gene            711..1796
                     /locus_tag="PK362_02295"
     CDS             711..1796
                     /locus_tag="PK362_02295"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNC73704.1"
                     /translation="MIYSRVVKPLLFRLSPEKAHALGMVALRAAAPFAFLGRWGRADD
                     PRLRVTLGRGADALTFPNPVGLAAGFDKDGVALPGLSALGFGFLEAGTVTPRPQPGND
                     RPRLWRFPAQRALINRMGFNNDGLDALAARWNRAPDLGIPVGANIGKNKNTPNDQAVD
                     DYLAGIDRLFDRADFFVVNVSSPNTPGLRDLLAPEQLGPLLGALRGRVDRWMSERRKP
                     KRPLLFVKISPDDSPGEALVETVVDAGFDGIVATNTTRDRAGLPPGAPAEGGVSGEPL
                     RARSTDIVRRLFRAARGRLALIGAGGGFSAADAYEKIQAGASLVEIYTGFVYGGPGVV
                     RNINRGLLKAMKDQKWDSIQNAVGSRA"
     gene            1793..2473
                     /gene="pyrF"
                     /locus_tag="PK362_02300"
     CDS             1793..2473
                     /gene="pyrF"
                     /locus_tag="PK362_02300"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNC73705.1"
                     /translation="MKRGGLIAALDVDAPAEARALVDRMGSAVDFYKVAPTMTLKDPG
                     FLAWLSARGKKTFLDCKWYDIPSQVQRSVQTAGELGVTAVTIHTGAGTAVMKAALAPA
                     RRPWVWGVTVLTSLGARDIQEVGVEVPPAEQVLRLARLAQAAGVDGLVCSPQEVSLLR
                     KNGITVTLVTPGIRFGGTVGGDQQRTATPGEAWSAGADYIVVGRSLLEAADPAATAKK
                     ILDERPSA"
     gene            2470..3012
                     /gene="pyrE"
                     /locus_tag="PK362_02305"
     CDS             2470..3012
                     /gene="pyrE"
                     /locus_tag="PK362_02305"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNC73706.1"
                     /translation="MNRQELAKTIYNACNIQGTFRLRSGQISSEYFDKYLLESRPEIL
                     KAVAEGMAALVPAETRILAGLEMGGIPVATAISFRTGLPVLFVRKKAKDYGTCKLAEG
                     IDFKGQRVTVVEDVVTTGGAIIDGVKALRDQGAIVHDVVCVIDRESGGAAKLGDVGLT
                     LRPLYRASELRTAAGAPAAT"
     gene            3028..3996
                     /gene="purM"
                     /locus_tag="PK362_02310"
     CDS             3028..3996
                     /gene="purM"
                     /locus_tag="PK362_02310"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNC73707.1"
                     /translation="MAKTSWTYKKAGVDIDAGDALVDRIRKKLPAIGDFAGLFPLPKG
                     LKKPYLVGCTDGVGTKLKIAQDLGVHNTIGIDLVAMNVNDLICCGARPLFFLDYFAAG
                     KLEVNVAEKVIGGMIDGCRQAGCALIGGETAEMPGMYPPGEYDLAGFAVGVVDDRDRV
                     RNEKIRPGDVLLGLPSSGVHSNGYSLVRVLFRGADLKKWGKALLAPTTIYVKPVLAAL
                     RKFPGAIKGLAHITGGGLLENVPRFLPKNTDAVFHVATWPLPPVFAEIRRRGNVADEE
                     MFRTFNMGLGMVLAVAPEKADAVRKALAPCFEVGRVESGRRRVRLT"
     gene            4094..>4655
                     /gene="purN"
                     /locus_tag="PK362_02315"
     CDS             4094..>4655
                     /gene="purN"
                     /locus_tag="PK362_02315"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNC73708.1"
                     /translation="MTDPGAIGVGVLVSGGGSNFEALAVAVREGRVPGAEIRLLISNK
                     PAVGALDRARRLGIEALVVEPREFPNRAAFYDRVAAEFERRGVQLVCLAGFLLKVEPN
                     FIAKFRGRILNIHPALLPQYGGAGMYGHHVHEAVIKAGERESGCTVHIVDEEFDHGPI
                     RMQSRVSVLPVDTPDSLAARVLAQEHK"
BASE COUNT          768 a         1465 c         1625 g          797 t
ORIGIN      
        1 gggttggccg ggttgtccgc gttgggctgg atggttgtcc ggtctccccg accccgggtg
       61 aacggagtcc aacgttccct gcttctgggc ggcggcggga tgatcctggg cgttttattc
      121 ctgcagagcg tgcattgggg ccgggaagac acaaagtttt caagcctcgt ctttcccgtg
      181 ttcctggggt tggcgttggg ggccgccgcc gtcgcccgcg ttccggcccg gcgaaacgcc
      241 ttgcgggggg cgctcctggc cttttgggcc gggaccttcg gctgcaacct gacggccgcc
      301 attgttccgg gcagccggct cgaaaacaac acggagttga tgaaggctct ttttgtccgg
      361 gaccacacgc ccgcgaacgc cgtggtggtg atcaacggcg ggatgcttcg tctctatctc
      421 ccgggcaacg ccgcccggca agccgtcgac ctggcctatc ccctggcgtt aacgtcgaaa
      481 gccggcgccc gggcctttgt ggaagatcaa attcgaacga cggtgctcgc cggccggccc
      541 ctgttcgccg tcaacgaggt cgtcgacgcg tcggcctggg ccgaaacgtc ggaccggggc
      601 tgggaccgga ccgaccggga ccggtggctg gcccccttcg acgtccagga aacggcccga
      661 aggaacgatt gggcgcttta cgccctccgc ccgcggaccg aggttcgacg gtgatctatt
      721 cccgggtggt gaaacccctt ttgttccgtt tgtccccgga aaaggcccac gccctcggca
      781 tggtcgcctt gcgcgcggcc gctccgttcg cttttttggg ccggtggggc cgggcggacg
      841 atccgcgact ccgggtcact ctgggccggg gcgcggacgc gctgacgttt cccaaccccg
      901 tgggcctggc cgcggggttc gacaaggacg gggtggcgtt gcccggcttg tcggccctgg
      961 ggtttggatt tcttgaggcc ggcacggtca ccccgcgccc tcagccgggc aacgaccgcc
     1021 cgcgtttgtg gcgattcccg gcccaacggg cgctgatcaa ccgcatggga ttcaacaacg
     1081 acggcctgga cgccttggcc gcccggtgga atcgcgcgcc ggatctcgga attcccgtcg
     1141 gggccaacat cgggaaaaac aaaaacacgc ccaacgacca ggccgtggac gattacctcg
     1201 cgggcatcga ccggcttttt gatcgggccg atttttttgt cgtgaacgtc agttccccga
     1261 atacgccggg gttgcgggac ctcctggccc cggaacagct ggggcccctg ctcggggccc
     1321 tgcggggccg cgtggaccgc tggatgtccg agcgacgaaa gcccaaacgg cccctcttgt
     1381 ttgtcaaaat atccccggac gattcgccgg gcgaggcgtt ggtggaaacg gtggtcgacg
     1441 ccgggttcga cggcatcgtc gccacgaaca cgacgcggga ccgggcgggg ttgcccccgg
     1501 gagcgcccgc cgaaggcggc gtgagcgggg aaccgctgcg cgcccgttcc acggacatcg
     1561 tccgccgtct ctttcgggcg gcccggggcc ggctggccct cattggggcg gggggggggt
     1621 tcagcgcggc ggacgcctac gagaaaattc aggccggggc ttccctggtg gagatctaca
     1681 ccggttttgt ttacggcggg cccggcgtcg tccggaacat caatcgagga ttgctgaagg
     1741 cgatgaaaga ccaaaaatgg gattccattc aaaacgcggt ggggagccgg gcgtgaagcg
     1801 cgggggcctg atcgccgcct tggacgtgga cgccccggcc gaggcccggg cgctggtgga
     1861 ccggatgggg tcggcggtgg atttttacaa agtcgcgccg accatgaccc tgaaagaccc
     1921 cggatttctg gcctggttgt cggcccgggg caaaaaaaca tttctcgact gcaaatggta
     1981 cgatatccct tcccaggttc agcgttccgt gcagacggcg ggggagctgg gcgtgacggc
     2041 cgtgaccatt cacaccggcg cgggcaccgc cgtcatgaag gcggccctgg cgccggcccg
     2101 acggccctgg gtgtggggcg tgacggtctt gaccagcctc ggggcccggg acatccagga
     2161 agtcggcgtc gaggttccgc ccgccgaaca ggtccttcgg ttggcccgcc tggcccaggc
     2221 ggccggggtg gacgggttgg tgtgttcgcc ccaggaagtt tctctgttac gcaagaacgg
     2281 aatcaccgtg accctcgtga cgccgggcat ccggttcggc gggaccgtcg ggggcgacca
     2341 acagcggacc gccacgccgg gcgaagcctg gtcggccggc gcggattaca tcgtggtggg
     2401 gcggagcctc ttggaagccg cggaccccgc ggccaccgcc aaaaaaatat tggacgaacg
     2461 accctcggca tgaatcgaca ggaattggcc aaaacgatct acaacgcctg caacatccaa
     2521 ggcacgttcc gccttcgaag cgggcagatc agctcggagt atttcgacaa gtacctgctc
     2581 gaatcccggc cggagatcct gaaggccgtg gccgagggca tggccgcgct ggtgcccgcc
     2641 gaaacgcgga tcctggcggg gttggagatg ggggggatcc cggtggccac cgcgatttcc
     2701 ttccgcacgg gattgcccgt tcttttcgtc cgcaaaaagg ccaaggacta cggcacctgc
     2761 aaattggcgg agggcatcga tttcaaaggc cagcgggtga cggtggtgga ggacgtggtc
     2821 accaccgggg gcgccatcat cgacggggtg aaggctctgc gggaccaggg cgcgatcgtc
     2881 cacgacgtgg tgtgcgtgat cgaccgggaa tccggcgggg ccgcgaagtt gggcgacgtc
     2941 ggcctgaccc ttcgccccct gtaccgggct tcggaactaa ggaccgccgc cggcgcgccg
     3001 gccgcgacat agaaagagag cgtttccatg gcaaagactt cctggacgta caagaaagcg
     3061 ggcgtggaca tcgacgcggg cgacgccctg gtcgaccgca tccgcaaaaa actgccggcc
     3121 atcggggatt tcgcggggct gttcccgttg cccaaaggcc tcaagaagcc gtatctcgtg
     3181 ggttgcaccg acggcgtggg caccaagctc aagatcgccc aagacctggg cgtgcacaac
     3241 accatcggca tcgacctggt ggccatgaac gtcaacgacc tgatttgttg cggagcccgg
     3301 cccctgtttt tcctcgacta tttcgccgcc gggaaactcg aggtcaacgt ggccgaaaaa
     3361 gtgatcggcg gcatgatcga cgggtgccgg caagccgggt gcgcgttgat cggcggcgag
     3421 accgccgaaa tgccgggcat gtacccgccg ggggaatacg atctggccgg tttcgccgtg
     3481 ggcgtcgtgg acgaccgcga ccgggtgcgc aacgaaaaaa tccggccggg ggacgttctg
     3541 ctcggacttc cctcctcggg ggtccactcg aacggctatt cgttggtgcg ggtgttgttc
     3601 cggggcgccg atttgaaaaa atggggaaag gcccttttgg cgccgacgac catttacgtg
     3661 aagccggtcc tggccgccct tcgaaaattc cccggcgcca tcaaaggcct cgcgcacatc
     3721 accggcggcg ggctgttgga gaacgtgccc cgctttttac cgaaaaacac cgacgcggtg
     3781 ttccacgtgg ccacctggcc cctgccgccg gtcttcgccg aaatccgccg ccggggcaac
     3841 gtggcggacg aggaaatgtt ccgcaccttc aacatgggat tgggcatggt cctggccgtg
     3901 gcgccggaaa aagcggacgc cgtgcgcaag gcgctggcgc cctgctttga agtggggcgc
     3961 gtggagtcgg gccgtcgtcg ggtccgtctg acttgacgcg gagggaccgc ctggacaaaa
     4021 agaccctccc gtattcgcac gggtcaaagc ttggtgcgaa cggtggccga gcggggacgg
     4081 gcgaggccat tccttgaccg atccgggcgc gatcggcgtc ggcgtcctcg tttccggcgg
     4141 aggatccaat tttgaagccc tcgccgtggc ggtccgggaa gggcgcgtcc ccggggcgga
     4201 aatccgcctt ctgatttcga acaaacccgc cgtcggagcc ctggaccggg cgcgccgact
     4261 gggcatcgag gcgctggtgg tggagcctcg ggaattcccg aaccgcgcgg cgttctacga
     4321 ccgggtcgcg gcggagttcg agcgacgcgg ggttcaactg gtctgcctcg ccgggtttct
     4381 gctgaaagtc gaacccaatt tcatcgccaa atttcgcgga cggatcctca acattcatcc
     4441 cgcgctgctg ccccaatacg gcggggcggg catgtacggc caccacgtgc acgaggcggt
     4501 gatcaaggcc ggcgaacggg aatcggggtg cacggtccac atcgtggacg aggaattcga
     4561 ccacggcccc attcgaatgc agtcccgggt gtcggtgttg cccgtcgaca cgccggactc
     4621 cctggcggcc cgggtgctgg cccaggaaca caagc
//
LOCUS       DAOQLS010000079         4646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8762_length_4646_cov_5.075583, whole genome shotgun sequence.
ACCESSION   DAOQLS010000079 DAOQLS010000000
VERSION     DAOQLS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8762_length_4646_cov_5.075583"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PK362_02320"
     CDS             complement(<1..756)
                     /locus_tag="PK362_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73709.1"
                     /translation="MNPTRWILSVFVLAGTLPVRAQETVVTLSQCQAWALQNSESRRI
                     SRESISQSESRARAALGGILPTVQWTWDRTWQDTSGLPVFNDPSGFSNSFALKRQDVS
                     KFTVDQALFGGFREFTARAGFLKERDRDALRLDHQSLEIFKSVMASFYDVRATETLRD
                     NVAEALALADRRTAELKSRYRLGKARESEMFSAEAQAASLRAQWIHLGGQTRWSRENL
                     SYVTGRDIASAPLADDETLSDRPAPLETFTGQLD"
     gene            complement(762..3896)
                     /locus_tag="PK362_02325"
     CDS             complement(762..3896)
                     /locus_tag="PK362_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC73710.1"
                     /translation="MTLSDLSIKNPVFAWMLMIGMIVFGLFGFTRMGVSQMPDVDFPV
                     LSVSVNWEGAAPEVMETEITDTLEDALISIEGLKEISSSSRQGSTSLTLEFNLDKDID
                     VALQEVQAKVAEAQRSLPREMDPPVIRKSNPEDQPIMWLALMGNRDRRFLMEYTRDQL
                     KDKFTTVPGVGEVTLGGYVEPNLRVWVNAEKLRAYELTVDDVLHAIGAQHAEVPAGRI
                     ETGRQELNVRVMGEATTVADFSNLIIPARGGAPLHRTVRLKDVAEVEDGLADLRRISR
                     AMGQSAVGLGIKKQRGANAVAVARAVKERMAQARKDLPEGLTLALNFDTTRFIEESTH
                     ELLLTLGLSALLTSAVCWLFLGSWSSAMNVILAIPTSIVGSFLILYFMGFTLNTFTLL
                     GLSLCIGIVVDDAIMVLENIVRYREKGLSRIKAAIVGAREITFAALAATVAILAIFVP
                     VVFMKGIIGKFFFQFGVTLSVAVVLSLLEALTLAPMRCSQFLEVGHSSRVGRAMDALL
                     ERLTRWYARGLDWSLRWRWWVCGSATFVFAASLFLVGGLKKEFVPSQDQSRFLVRLQT
                     PLGSSIESTDAVFKKAEDIVSARPELERYFGAIGGFGGGEVNTAMMFISLKDPAKRPV
                     TPPYKGPVTQSDVMEYVRGQFKAIPGVQRVAVQDLSQQGFSAQRGFPVEFTIRGPDWD
                     KLGELAGVFEKRMEATGLMIDADTDYRVGMPEIRVVPDRDKSSLRGVTVESIANTINA
                     MIGGVRAGKYTKGGKRYDIRLRLVADERRRPDDIRKIYVRNDRGEVIPLSQVVAINEK
                     PTLLTINRRDRERAVSLFANVAPGKSQGEAINAVNALGREILPEGYRLVLSGSAKTFK
                     ESFSSLVFALVLGLFVAYMVLGAQFNSFIHPVTVLLALPFSVTGAFLALRMTGISLNV
                     YSLIGLLLLMGLVKKNSILLVDFTNERRVRGLGVREALLDACPVRLRPILMTSVSTIV
                     GAIPAALAFGPGAETTRPMAVVVIGGMLVSTVLTLFVVPSAYSLLANLQSHAHDRDLK
                     EALKELGELKEAVE"
     gene            complement(3893..>4646)
                     /locus_tag="PK362_02330"
     CDS             complement(3893..>4646)
                     /locus_tag="PK362_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73711.1"
                     /translation="VRIGRSREGELLSVRSQRATLEARRRRAEGLRASALEVLSVLVG
                     ESVGAVALEPGEPPPADSLARWIEKLPTRPDLAAAERSAEAAEFLATARRREKWPSLV
                     LAGNYYTQRSGSNAAVDWDSTLSLDLPLFRGGAWRYGVRVAAEESAAARWRLSRERRA
                     AESELRARYAELLSARSETEALLSAAELAEANYNAQVRDYGLGLVTNLDVLTAMDTFQ
                     EAKLSYDRRRLDVRLGALRLAVAAGVVPEAAP"
BASE COUNT          926 a         1464 c         1452 g          804 t
ORIGIN      
        1 gtccaattga cccgtgaacg tttccagggg ggcgggccgg tccgacaacg tttcgtcgtc
       61 ggccagcggg gccgaggcga tgtcgcgtcc ggtcacgtag gaaagatttt cccgggacca
      121 ccgcgtctgg cctcccaggt gaatccattg ggcccgcaac gacgcggcct gggcttcggc
      181 gctgaacatc tcgctttccc gggcctttcc caaccggtag cggctcttga gctcggcggt
      241 ccgccggtcg gcgagggcca gggcctcggc cacgttgtcc cgcagggttt cggtcgcccg
      301 cacgtcgtag aacgacgcca tcaccgactt aaagatctcc agcgactgat ggtccaatct
      361 caaggcgtcc cggtcccgtt ctttcaagaa accggcgcgg gcggtaaatt cccgaaaccc
      421 cccgaagaga gcctggtcga cggtgaactt cgacacgtcc tgccgtttta aggcgaaact
      481 attgctgaag ccggagggat cgttgaacac gggaagacca ctcgtatcct gccaggttcg
      541 atcccaggtc cattgaaccg tggggagaat tccgcccaga gcggcccgag cccgggattc
      601 ggattgtgaa atcgactcgc gggaaatccg ccgcgattcg ctgttttgaa gggcccaggc
      661 ctggcattgg gacaaggtga cgacggtctc ctgggcacgg accggaagcg tccccgccaa
      721 aacgaaaacc gagagaatcc atcgcgtagg gttcatcgac attactccac cgcttccttg
      781 agttctccca attccttgag cgcttccttt aaatcccggt cgtgggcatg gctctgaaga
      841 ttggccagaa gactgtaggc gctggggacc acaaaaaggg tcaggacggt cgacaccagc
      901 atgccgccga tgacgaccac cgccatgggg cgagtcgttt cggccccggg cccgaaggcc
      961 aaagccgccg ggatggctcc cacgatggtg gacacggagg tcataaggat ggggcgaagg
     1021 cgcaccgggc aggcgtccag aagggcctcg cgaacgccca gaccgcgcac ccggcgttcg
     1081 ttcgtaaaat ccacgaggag gatggagttc tttttcacca gtcccatgag caggagcagg
     1141 ccgatcaggc tgtaaacatt caaggaaatg cccgtcattc gaagagccag aaaagcgccg
     1201 gtcacgctga agggcaacgc caataaaacg gtcaccgggt gaataaaact gttgaactgg
     1261 gcgcccagga ccatgtaggc cacgaaaaga cccaggacca aagcgaagac caggctggag
     1321 aaggactctt taaaggtttt ggcgcttccg gaaagaacga ggcgataccc ctcgggcaga
     1381 atctcccgcc ccagggcgtt gaccgcgttg atggcctccc cctgggattt cccgggagcc
     1441 acgttggcga agaggctcac ggcccgttcc cggtcccgcc gattgatggt cagaagcgtc
     1501 ggcttctcgt tgatggccac gacctgggac aaggggatta cttcgccccg gtcgttccgc
     1561 acataaattt tccgaatgtc gtcggggcgc cggcgttcgt cggccaccag gcgcaagcgg
     1621 atgtcgtacc gtttgccgcc cttggtgtac ttgccggccc gcaccccgcc gatcatggcg
     1681 ttgatggtgt tggcgatgga ttcgaccgtg accccccgaa gggaactttt gtcccggtcc
     1741 ggaacgacgc gtatttccgg catgcccacc cggtaatcgg tgtcggcgtc gatcatcaaa
     1801 cctgtcgctt ccatccgttt ttcgaacacc ccggccaact cgcccaattt atcccaatcg
     1861 gggccccgaa tggtgaactc caccgggaaa ccgcgctggg cggagaatcc ctgctgggac
     1921 aaatcctgga cggccacccg ttggacgccg ggaatggcct tgaactggcc ccgaacgtat
     1981 tccatcacgt ccgactgggt cacgggaccc ttgtacgggg gcgtgacggg gcgtttggcg
     2041 gggtctttga gggaaatgaa catcatggcg gtgttgactt cccctccccc gaaaccgccg
     2101 atggcgccga agtagcgttc cagttcggga cgggcggaaa cgatgtcttc cgccttttta
     2161 aatacggcgt ccgtggattc gatcgaggac cccaagggcg tctgcaatcg caccaagaat
     2221 cggctttggt cctgggacgg cacgaactcc ttttttagcc cgccgaccag gaacagcgaa
     2281 gcggcgaaga cgaaggtggc gcttccgcac acccaccacc gccaacggag gctccaatcc
     2341 aatccccggg cgtaccaccg ggtcaatcgt tccaggagcg cgtccatcgc ccgccccacc
     2401 cgggaggaat ggccgacttc caaaaactgc gaacagcgca tgggcgccag ggtgagggcc
     2461 tccagcaggg acagaacgac ggcgaccgag agcgtgacgc cgaattggaa aaagaatttc
     2521 ccgatgatcc ccttcatgaa aacgacgggg acgaaaatgg ccaagatggc gacggtggcg
     2581 gccagggcgg cgaaggtgat ctcccgggcc ccgacgatgg cggctttgat gcgcgagagc
     2641 cccttctccc gatagcggac gatgttctcc aacaccatga tggcgtcgtc gaccacgatg
     2701 ccgatgcaga gggaaagtcc gaggagggtg aaggtgttca gcgtgaaacc catgaaatac
     2761 aaaatcaaaa aggagccgac gatggaggtg ggaatggcca gaatcacgtt catggccgag
     2821 ctccaggacc ccaaaaagag ccagcagacc gcggaggtga gcagggccga aaggcccaga
     2881 gtcagcaaaa gttcgtgggt ggattcctca atgaaccgcg tggtgtcgaa attcaacgcg
     2941 agcgtcaaac cttcgggcaa gtctttccgg gcttgcgcca tgcgctcctt gaccgcccgg
     3001 gccacggcca cggcgttggc gccccgctgc tttttgatgc cgaggcccac ggccgattgg
     3061 cccatggcgc gggagattcg gcgaagatcg gccaacccgt cctccacctc ggccacgtct
     3121 tttagccgaa ccgtccggtg aagaggcgcg cccccccggg ccggaatgat caaattcgaa
     3181 aaatccgcca cggtggtcgc ttcgcccatg acgcgcacgt tcagttcctg ccggccggtt
     3241 tcgatgcgcc ccgccggcac ctcggcgtgc tgggcgccga tggcgtgcag gacgtcgtcc
     3301 accgttaatt cgtaggcccg aagtttttcg gcgttgaccc acacccgcag gttgggttcc
     3361 acgtagccgc cgagcgtgac ctcgcccacc ccggggaccg tcgtgaattt gtccttcagc
     3421 tggtcccggg tgtattccat gagaaatcgc cggtcccggt tgcccatgag cgccagccac
     3481 atgatcggct ggtcctcggg attggatttg cggatcacgg gcgggtccat ctcgcgggga
     3541 aggctgcgtt gggcttcggc caccttggcc tggacttcct ggagcgccac gtcgatgtct
     3601 ttgtcgaggt tgaattccaa ggtcagggac gtcgacccct ggcgggagct cgaggatatt
     3661 tccttcaacc cctcgatgga aataagggcg tcttcgagcg tgtcggtgat ttccgtttcc
     3721 atcacttcgg gagcggcccc ttcccagttg acgctcaccg atagaacggg gaaatccacg
     3781 tcgggcattt ggctgacgcc catgcgggtg aaaccgaaca ggccgaagac gatcatcccg
     3841 atcatcaaca tccaggcgaa aaccgggttc ttgatggaca aatccgacag ggtcatggcg
     3901 cggcctccgg gacaacgccg gccgccacgg ccaggcggag ggcccccaac cgaacgtcca
     3961 accgtcgacg gtcataggaa agtttcgctt cctggaaggt gtccatggcg gtcaggacgt
     4021 ccaaattggt cacgaggccc aggccgtagt cccgcacctg ggcgttgtag ttggcctcgg
     4081 ccaattccgc ggccgacaaa agggcttcgg tttccgagcg ggccgaaagc agctcggcgt
     4141 atcgggcccg aagttcggac tccgccgccc ggcgctcccg ggacaaccgc cagcgggcgg
     4201 ccgccgattc ttccgcggcg acgcggacgc cgtaacgcca ggccccgccc cgaaagaggg
     4261 gcaggtccaa ggacaacgtc gaatcccaat cgacggcggc gttggacccc gaccgttggg
     4321 tgtagtaatt gcccgccaaa acaaggctgg gccacttttc acgtcggcgg gccgtcgcca
     4381 gaaattccgc cgcctccgcc gaccgctccg cggccgccaa atcgggccgg gttggaagtt
     4441 tttcgatcca acgggcgaga gagtcggccg ggggaggttc cccgggctcc aacgccacgg
     4501 cccccaccga ttcgccgacc aaaacgctta gaacttccaa ggccgaggcg cgaagccctt
     4561 cggcccgccg tcggcgggct tccagggtgg ctcgctggga acgaaccgac aagagctccc
     4621 cctcccggga ccggccgatg cggacc
//
LOCUS       DAOQLS010000080         4646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_8763_length_4646_cov_5.075147, whole genome shotgun sequence.
ACCESSION   DAOQLS010000080 DAOQLS010000000
VERSION     DAOQLS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8763_length_4646_cov_5.075147"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PK362_02335"
     CDS             <1..527
                     /locus_tag="PK362_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73712.1"
                     /translation="RGVLQILILSGMIAVGCRRTEAPPAPPAFDPVALEARFPADLGT
                     ATLDVSGYPPEKKSHYALFSEKCAGCHSLARPLNSPWTTEVDWRRYVERMETNNEDKT
                     GKPLLTPVEAKKIISFLVYDSRERKMKNRAGFEAEQARLKDLYAKVVAERNRLAAAQS
                     KSQGRESSPYVGDR"
     gene            564..905
                     /locus_tag="PK362_02340"
     CDS             564..905
                     /locus_tag="PK362_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC73713.1"
                     /translation="MKKQAVVWVLALGLGAGALAARAEDAGKIYAAKCQSCHGKDGKG
                     NPAMTKVLKADLAALSLVDDATLAKTDADLVKITLEGVGKMPKYQGKLTEADVKAVTA
                     YVRAMKPAPAK"
     gene            936..1268
                     /locus_tag="PK362_02345"
     CDS             936..1268
                     /locus_tag="PK362_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC73714.1"
                     /translation="MKRLSLLLGVLTLLAPVAGAEDAAKLYGAKCAGCHGKDGKGNAV
                     MVKALKVDLAALSLVDAASLAKKDEEWVKLTLDGQGKMPAQKGKIKDEDVKALVAYVR
                     GLAPKSAQ"
     gene            1297..3192
                     /locus_tag="PK362_02350"
     CDS             1297..3192
                     /locus_tag="PK362_02350"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNC73715.1"
                     /translation="MRRHRLFPRGFWERAALAGALLLFGGIGRLRADGNEDCLACHGN
                     ADSGAPPVHEKAFARSIHGRNLCVSCHVDATEIPHAEKLAPVSCARCHRLETQIYLNS
                     DHGRAVARGKVEAASCKDCHGHSHTLLNSRNPDSPVNRKNIHDTCAACHGKADAPANQ
                     NLTERKPVDSYDHTVHGVAFRAGKINAAVCSDCHGTHDLHGSANPASRVNRARIPETC
                     GRCHQNVLSVYQVSIHGRASANGIKETPVCTDCHGEHTIQSPENPASSVFRGAVTKTC
                     SGCHESERIVAKFGLPAGRLKSFMDTYHGLASQRGDLRVANCASCHGWHDVLPSTDPQ
                     SAVNPANLANTCGRCHAGAQAKLLAGRIHAGKGNRPNRWVLFFRWFYLIAIPLTIGGM
                     LVHNGLDFLRKVIDPPPPPHGRELTVLRLTAHERAQHGLLALAFSVLAYSGFALAFPS
                     AWWAAPFQWAGGEVARKALHRWTALGFVLMGGWHAAYMIGTARGRFLLREGLLPRLRD
                     LTEPLHLTLYNLGLRKTRPALRYPTYIERAEYWALLWGSLVMVVTGGTLVFNNFTLRH
                     APLWVPDLATMIHYYEAILACLAILVWHAYWTVFDPAVYPMNWAWLIGRLRRKKAAAP
                     EKPHGAK"
     gene            3179..4078
                     /locus_tag="PK362_02355"
     CDS             3179..4078
                     /locus_tag="PK362_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNC73716.1"
                     /translation="MERNRAVLLLAAVLGGPPALRAQYAGPEACADCHPQEFQDWKPS
                     VHARAYASDTFQRTWKENGSTPACLKCHTTGWKSGTSVYAHPGVTCESCHGALAEGHP
                     AERMPLPGGSDMCRDCHKKSHGEWKLSRHGQKNIRCFDCHKVHGQGLRLGGGDALCGS
                     CHAAKLKDFAHATHHQEGLSCNTCHFPKSSAGPDAIMGTGAPGHNLSVGPQVCDRCHE
                     ETVHKSGRLADLRSRMSQTEKHMAVAGVTSVFELNEKAKDLEWRLERARSNAWLVALL
                     GLLAGLGLGWLAGWYVFRPGAKK"
     gene            4075..>4646
                     /locus_tag="PK362_02360"
     CDS             4075..>4646
                     /locus_tag="PK362_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC73717.1"
                     /translation="MTVEVSRRSLLKGMVGAVAAVGLPRSMARAQSPVAKSDVFSVLV
                     DIPRCIGCRACLRGCQRANDLDAPQDLMDMDHLASRLEPAHNLWTVVNREGRETVRHV
                     KRQCMHCLEPACVSVCPVAALHQTDAGPVLYRENRCIGCRYCMVACPFDVPRFEWNNG
                     LTPVIGKCQFCASQRLFKGLPPGCVEACPTG"
BASE COUNT          858 a         1532 c         1477 g          779 t
ORIGIN      
        1 gccgcggcgt cctccaaatc ttgattttgt cggggatgat cgccgtcggt tgccgcagga
       61 ccgaagcgcc cccggcgccg cccgccttcg acccggtggc gctcgaggcc cggttcccgg
      121 cggacttggg aacggcgacc ctcgacgttt cgggctatcc acccgaaaag aagtcccact
      181 acgccttgtt ttccgaaaaa tgcgccgggt gccactccct ggcccgtccc ctcaattcgc
      241 cctggaccac cgaggtggat tggcgccgct acgtggagcg gatggaaacc aacaacgagg
      301 acaaaaccgg caaacccctg ctgacgcccg tggaggcgaa aaaaatcata tcgttcctgg
      361 tgtacgacag ccgggaacga aagatgaaaa accgggccgg gttcgaagcg gagcaggcgc
      421 gcctcaaaga cctttacgcc aaagtcgtcg cggagaggaa ccgcctggcg gcggcccaaa
      481 gcaaatccca gggtcgggaa tcgtccccgt acgtcgggga ccgttgacgt cttcaattta
      541 atctctcagg aggaatcccc atcatgaaaa aacaagcggt ggtctgggtg ttggctttgg
      601 ggttgggcgc gggcgccctg gcggcgcggg cggaggacgc ggggaaaatt tacgcggcga
      661 aatgccaatc ctgccacggc aaagacggca aaggcaatcc ggccatgacg aaggtgctga
      721 aggccgacct cgcggccttg agcctggtgg acgacgccac cctggccaaa accgacgcgg
      781 atttggtgaa gatcaccctg gaaggcgtcg ggaaaatgcc caagtatcag ggcaagttga
      841 ccgaagcgga cgtgaaagcg gtgacggcct acgtgcgcgc gatgaagccc gcgcccgcga
      901 aataaccttt taatttctcg agtcaaggag ttcctatgaa acgattgtca ttgttgttgg
      961 gggtcctgac ccttcttgcc ccggtcgccg gagccgaaga cgccgccaag ctttacgggg
     1021 ccaaatgcgc cggatgccac ggcaaggacg gaaagggcaa cgcggtcatg gtcaaagccc
     1081 tcaaggtgga tctggcggcg ttgagcctgg tggacgcggc gagtctggcg aaaaaagacg
     1141 aggagtgggt caagctcacc ctcgacggcc aggggaaaat gccggcccaa aaaggcaaaa
     1201 tcaaagacga ggacgtgaaa gccctggtgg cctacgtccg cggtttggcg ccgaagtcgg
     1261 cccaataagc ttccaacgag attgagcgga gcgcccgtgc gccggcaccg ccttttcccg
     1321 cgtggttttt gggaacgggc ggcgctggcc ggggcgctcc tcctgtttgg gggaatcggg
     1381 cgtcttcggg ccgacggcaa cgaggattgc ctggcgtgcc acggaaacgc cgattccggc
     1441 gctccccccg tccatgaaaa ggccttcgcc cgctccatcc acgggcggaa cctgtgcgtt
     1501 tcctgccacg tggacgccac cgaaattccc cacgccgaaa aactcgcgcc ggtgtcctgc
     1561 gcccggtgcc atcgtctcga aacgcagatt tacctcaatt ccgatcacgg tcgcgccgtc
     1621 gcccggggaa aagtggaggc ggcgtcctgc aaggattgcc acggtcacag ccacaccctg
     1681 ttgaactccc ggaaccccga ctcgccggtc aaccgcaaaa acattcacga cacctgcgcc
     1741 gcgtgccacg ggaaagccga cgcgccggcc aaccagaatc tgacggagcg gaaacccgtc
     1801 gacagttacg atcacacggt ccacggcgtg gcgttccgcg cgggcaaaat caacgcggcc
     1861 gtctgctccg actgccacgg cacccacgat ttgcacggct ccgccaaccc ggccagccgg
     1921 gtgaaccggg cccgcattcc cgaaacctgc ggtcggtgcc accaaaacgt tctttccgtt
     1981 taccaggtca gcatccacgg ccgcgccagc gccaacggga tcaaggaaac ccccgtgtgc
     2041 accgactgtc acggcgaaca caccattcaa tcgccggaaa atccggcgtc ctccgtgttt
     2101 cggggcgcgg tcaccaaaac ctgctcgggg tgccacgaat ccgagcgcat cgtggccaaa
     2161 ttcggccttc ccgccggccg gctgaaaagt ttcatggaca cctaccacgg cctggcctcc
     2221 caacgggggg atctgcgggt cgccaattgc gcctcctgcc acgggtggca cgatgtcctg
     2281 ccgtccaccg atccccaatc cgccgtgaac cccgccaatt tggccaacac ctgcggacgt
     2341 tgccacgccg gggcccaggc gaaattgctc gccggacgca tccacgcggg caaagggaac
     2401 cgcccgaacc ggtgggtgtt gttttttcga tggttttacc tgatcgccat cccgctcacc
     2461 atcggcggga tgttggtgca caacgggttg gattttcttc gcaaagtgat cgacccgccg
     2521 cccccgcccc acggacggga actgacggtc ctgcgtctga ccgcgcacga gcgcgcccag
     2581 cacgggctcc tggcgctggc cttttcggtg ctggcctaca gcgggttcgc cctggcgttc
     2641 ccgagcgcct ggtgggcggc gccgttccaa tgggccggcg gagaggtcgc ccggaaggcc
     2701 ctgcaccgct ggaccgccct gggcttcgtg ttgatggggg ggtggcacgc cgcgtacatg
     2761 atcggcaccg cccggggacg ttttctcctt cgcgaagggc tcctcccgcg tttgcgcgat
     2821 ctcacggaac ccctccactt gacgctgtat aacctgggcc ttcggaagac ccggccggcc
     2881 ctccgttacc ccacctacat cgagcgggcc gaatactggg ccctcctctg ggggtccctg
     2941 gtgatggtgg tcacgggggg cacgttggtc ttcaacaatt tcacgctccg ccacgcgccg
     3001 ctctgggtgc cggatttggc gacgatgatc cactactacg aagccatcct cgcctgtctg
     3061 gcgattttgg tgtggcacgc ctattggacg gtgtttgatc ccgcggtcta ccccatgaat
     3121 tgggcgtggc tcatcggacg ccttcgccgg aaaaaagcgg ccgccccgga gaagccccat
     3181 ggagcgaaat agggccgtcc ttctcctggc ggcggtcctg ggcgggcccc ccgcgctccg
     3241 ggcccagtac gcgggccccg aggcctgcgc cgactgccac ccccaggaat ttcaggattg
     3301 gaaaccgtcg gtccacgcgc gggcctacgc gtccgacact tttcaacgga cgtggaagga
     3361 aaacggttcc acccccgcct gcctgaagtg ccacaccacc ggttggaaat cgggcacctc
     3421 ggtatacgcg cacccggggg tcacctgcga atcctgccac ggcgccctgg cggaggggca
     3481 ccccgccgaa cgcatgcccc tgcccggcgg gtccgacatg tgccgggact gccacaaaaa
     3541 atcccacggc gaatggaagc tgagccggca cggccaaaaa aacatccggt gctttgactg
     3601 ccacaaggtc cacgggcagg ggctgcggtt gggcggcggc gacgcgctgt gcgggtcctg
     3661 tcacgccgcg aaactgaagg atttcgccca cgccacccac caccaggaag ggttgagctg
     3721 caacacctgt cattttccca aatcgtcggc gggccccgac gccatcatgg gcacgggagc
     3781 gccgggccac aacttgtccg tcggccccca ggtgtgcgat cgatgtcacg aggaaacggt
     3841 tcacaaaagc ggccggctgg cggaccttcg ctcccgcatg agtcaaaccg aaaaacacat
     3901 ggccgtggcg ggggtgacca gcgttttcga actgaacgag aaagccaagg atttggaatg
     3961 gcgtttggag cgggcgcgct ccaacgcgtg gctggtcgcg ttgctcggcc tgttggccgg
     4021 gttgggcctg gggtggttgg ccgggtggta cgttttccgg cccggggcca aaaaatgacc
     4081 gtcgaggtgt cccgccgctc cctgctgaag ggaatggtgg gcgcggtcgc cgcggtgggg
     4141 ttgccccgtt ccatggcccg ggcccagtcc ccggtggcca aaagcgacgt gttcagcgtt
     4201 ctggtggaca ttccccggtg catcggttgc cgggcctgtc tgcgcgggtg ccagcgggcc
     4261 aacgacctcg acgcgcccca agacctcatg gacatggacc acctcgcgtc gcgcctcgag
     4321 cccgcccaca acctgtggac cgtggtcaat cgcgaagggc gggaaaccgt tcggcacgtc
     4381 aagcgccaat gcatgcattg cctggaaccg gcctgcgtgt cggtgtgccc cgtggcggcc
     4441 cttcatcaaa cggacgcggg ccccgttctt taccgggaaa accggtgcat tgggtgccgt
     4501 tactgcatgg ttgcctgccc tttcgacgtc ccgcggttcg aatggaacaa cggcctgacg
     4561 cccgtgatcg gcaaatgcca gttctgcgcc tcccagcgcc ttttcaaagg gctgccgccc
     4621 ggttgcgtgg aagcgtgccc gacggg
//
LOCUS       DAOQLS010000081         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9030_length_4563_cov_3.568545, whole genome shotgun sequence.
ACCESSION   DAOQLS010000081 DAOQLS010000000
VERSION     DAOQLS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9030_length_4563_cov_3.568545"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4545)
                     /locus_tag="PK362_02365"
     CDS             complement(<1..4545)
                     /locus_tag="PK362_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73718.1"
                     /translation="MTTYLDESEQKGVFVEKTGRSTSDTSSTYISIWGQAVVSQAVTT
                     TVTESGATRSTSTITVNYSYNASGQLIAAEGHGTSHSETSVWNDTNNDGVHDVGELEL
                     QVTDAVITNTYDIIGGQAFVSSSTSDSTTVAGASTTHSVSVTFTLYNEKGQVIGARGE
                     STTDNTTKTLTVKAGEVARESSQTNHSHTVSDYIVVGGQAVVSRSKTTSTMVNGNVTT
                     ESTSYMNYHYNALGQLDSATGSSSGTNKTRVHSEPDNDPATIDPPEWSDQISDFSSTN
                     VYGIIWGQAQLLESTTTSGSATGTSESETTSHVAYRYDSQGRLVSATGTSHTTSTNDV
                     FNDANGNKQKDAGEVVKQQTTSDAVNTYAIHWGQAVVTQSVTDQTTRTGMINNDPATA
                     TTVTTSKAYMFYTYNDLGQLTGVTGYSDGNVHQQHINKVPRVVSTTSFDSEGFSAYLS
                     TCISEALSTATDLWMVGGASQALARGLSKAAANLNAFYTTTSSVVYDNVVTYTDTPQH
                     TENTFDIINGQAVMVRTTTHSTAPAGATNSTSDATTSYTYDYNSGALLHVTGDNDSHS
                     TTTFADGTTSTTTSHTANEFLVRQGQALLMSATTTSTTDGPGNHSTGTTTTRYTYDER
                     GMMIGARGTTSSDTTTQVYTNGVLENQTNHTTGTNTYVVLWGQAMLLENISHSENNFK
                     NVNTVTDSVVRYSYNAQGQMIGAIGFNDATTTTTNADGSKTYTSNHVDNDYAVYFGQA
                     QIAASIGRTSSHSGDLTAGESAAIAPVIGKSPLGRQNGVVDTGAASWTVYQYNALGQI
                     TGALGGSKSITQTLNKDTTISVSVANTRNDYIVVGGQALVTQSRTHSTAYSGFDRNTA
                     GWNLLSASSITVSDTIMTNTYDAVGRLTGAGAQIINGVTTTTNSDGTRSISHFHGTNQ
                     YAIIGGQAVVINAQSDSTTVSGATTTTSHSNTDYTYNSLGQLVSATGTSTGSSDTTNI
                     EKSIDANGNATYSTSVTHTTMTSEMTYRVVAGQALVTKITTTSHTTGADSTSDTTSDT
                     FNEYDARGLLVSSYSTSTTDSVTKIWDRDLNGNKVFDANEEGQQTAHITSRTQNVIVW
                     GQAVASRTTSHTHSATEGNINVTDSDSDVSYTYNARGQLTGAVAQPTTGSTTILNADG
                     STTVTRFTTTNVYEVRQGQALVSRSSTHSTTTADRTTTTTDSVTSYQYNALGQLTGAN
                     SSADSTSITQVPDSRYGSMERRDISHTDTAMAIHNGQAVAGKSVTTSDSYSGYSGSNY
                     LAAVSHTHNTSWTVNDYNGRGQLIRTTGGSEGYSDKKNSDNTVTHSVLSGDNTYVIIG
                     GQSVVSASHSLSTTYSAYDGTNYNTARDISTNESNTGYALDRYGRVTGGGTTFSSRQA
                     TVDSDGRSWTYSFTNGWTTLIAVGGQTYEKHTESTTTHSNSTGSTTNVVDYIYSGSRL
                     VDATGYTTGTTGEAVWNDINHDGNWTPNETVIETGNIRTDNTYIIVNGAAEVSHSHTH
                     TWGSSGDSSRDSHQYMDFGFN"
BASE COUNT          689 a         1186 c         1587 g         1101 t
ORIGIN      
        1 gttgaatcca aaatccatgt attgatggga gtcgcgcgaa gagtcgcccg aggatcccca
       61 cgtgtgggtg tgggaatgag agacttcggc ggctccgttc acgatgatgt aggtgttgtc
      121 ggtgcgaata ttgcccgttt caatgaccgt ttcgttgggc gtccaattgc cgtcgtgatt
      181 aatgtcgttc caaacggctt ctccggttgt gcccgtcgtg tatcccgtgg cgtcgaccag
      241 gcggctgccg gaatagatgt agtccaccac attggtggtg gatcccgtgg agttggagtg
      301 ggtggtcgtc gattccgtgt gcttctcgta ggtttgaccc cccaccgcga tcaaggtcgt
      361 ccacccgttg gtaaacgagt aggtccacga acgcccatcg gaatccaccg tcgcctggcg
      421 cgaggagaaa gtcgttcctc cgcccgtgac ccgaccgtaa cggtccaagg cgtatcccgt
      481 gttcgattcg ttggtcgata tgtcccgggc ggtgttgtag ttcgtgccgt cgtaggcgct
      541 gtaagtggtg ctgaggctgt gcgacgcgga aacgacggat tggccgccga tgatgacgta
      601 ggtgttgtcg cccgacagga cggaatgggt gacggtgttg tcgctgttct ttttgtcgct
      661 gtaaccttcg gagcccccgg tggtgcgaat cagctggccc cggccgttgt agtcgttgac
      721 ggtccacgag gtgttgtgcg tgtggctgac ggccgcgagg taattggaac cgctgtagcc
      781 cgaataggaa tccgacgtgg tgacgctctt gcccgccacg gcctgcccgt tgtggatcgc
      841 catggcggtg tccgtgtggg agatgtcccg gcgttccatg ctgccgtatc ggctgtccgg
      901 cacctgggta atcgaggtgc tgtcggccga actgttggcc ccggtcaatt gccccagggc
      961 gttgtattga tagctggtca cggaatccgt cgtggtggtg gttcggtcgg ccgtggtcgt
     1021 ggagtgggtg ctcgaccgcg aaaccagggc ctgcccctgc cgcacctcgt acacgttggt
     1081 ggtggtgaac cgcgtcacgg tggtggaccc gtcggcgtta aggatggtcg tgctaccggt
     1141 ggtcggctgc gccaccgcgc cggtcaactg gccccgggcg ttgtaggtgt aggacacgtc
     1201 cgaatccgaa tcggtgacgt tgatgttgcc ttcggttgcc gagtgggtgt gggacgtcgt
     1261 ccgggaggcg accgcctgtc cccacacgat cacgttttgg gtgcgggacg tgatgtgcgc
     1321 ggtttgttgg ccctcttcgt tcgcgtcgaa tactttgttg ccgttcaggt cgcggtccca
     1381 gatcttggtc accgaatcgg tggtggacgt ggaatacgac gagaccaata agccccgggc
     1441 gtcgtattcg ttgaacgtgt cgctcgtggt gtcggaagtg gaatccgcgc cggtcgtgtg
     1501 ggacgtggtc gtgattttgg tcacgagggc ctgcccggcc accacgcggt aggtcatttc
     1561 cgacgtcatg gtggtgtggg tgaccgacgt ggaataggtg gcgttcccgt tcgcgtcgat
     1621 ggatttttcg atgttggtgg tgtcgctgct cccggtggag gtgcccgtgg ccgaaaccag
     1681 ttggccgagg ctgttgtaag tgtaatcggt gttcgaatgg ctcgtcgtcg tggtggcgcc
     1741 cgacaccgtg gtcgaatcgc tctgggcgtt gatcaccacc gcctgcccgc cgatgatcgc
     1801 gtattgattt gtaccgtgga aatgtgaaat ggagcgcgtc ccgtcggaat tcgtcgtggt
     1861 cgtgaccccg ttgatgattt gcgcgccggc gccggtcagt cgaccgacgg cgtcgtaagt
     1921 gttcgtcatg atcgtgtccg acaccgtgat ggaagaggcc gacaggaggt tccagccggc
     1981 cgtgttccgg tcgaatccgc tgtaggcggt ggaatgggtg cggctttggg tgaccagggc
     2041 ctgccctccg accacaatgt aatcgtttcg cgtgttggcg acggacaccg aaatcgtcgt
     2101 gtccttgttc aaggtctgag tgatggactt ggaaccgccc aacgccccgg ttatttgtcc
     2161 cagggcgttg tattggtaga ccgtccagct ggccgcgccg gtgtccacga cgccgttttg
     2221 acggccgagg ggcgacttgc cgatgacggg agcgatggcg gcgctttccc cggcggtgag
     2281 atctccggaa tgggaggacg tccgcccgat ggaagcggca atctgggcct ggccgaagta
     2341 aacggcgtaa tcgttgtcga cgtggttgga ggtgtaggtc ttcgagccat ccgcgttcgt
     2401 ggtcgtcgtg gtcgcgtcgt tgaatccgat cgccccgatc atttgtccct gggcgttgta
     2461 ggaataccgg acaacgctat cggtgaccgt gttgacgttt ttgaagttgt tttcgctgtg
     2521 ggaaatgttt tccagcaaca tggcctggcc ccacagaacc acataggtgt tcgtccccgt
     2581 ggtatggttg gtctgatttt ccaagactcc gttcgtatac acctgagtcg tggtgtcgct
     2641 gctggtggtc ccgcgggcgc cgatcatcat cccccgctca tcgtaggtgt agcgggtcgt
     2701 ggtggtcccg gtggaatggt tgcccgggcc gtccgtcgtc gaggtcgtgg tcgccgacat
     2761 caggagcgcc tgcccttgac gcaccaggaa ttcattggcg gtgtgcgagg tggtcgtgga
     2821 cgtcgtgccg tccgcaaacg tcgtcgtcga atggctgtcg ttgtcgcccg tcacgtgaag
     2881 aagggccccg ctgttgtagt catacgtgta ggacgtggtc gcgtcgctgg tggaattggt
     2941 ggcgccggcg ggggccgtgg agtgggtggt ggtccgaacc atgacggctt ggccgttgat
     3001 gatgtcgaag gtgttctccg tgtgttgcgg cgtgtcggtg taagtgacga cgttgtcgta
     3061 caccaccgaa ctggtggtgg tgtagaacgc gttcaggttc gcggccgctt ttgaaaggcc
     3121 gcgcgcaagg gcttgagaag ccccgccgac catccacaag tcggtggcgg tggagagggc
     3181 ttcggagatg cacgttgaaa ggtaagcgga aaaaccttcg ctgtcaaagg acgtggtcga
     3241 aacgacccgg ggcaccttgt tgatgtgttg ctggtgaacg ttcccgtcgg agtagccggt
     3301 gacccccgtc aattggccca ggtcgttgta ggtgtagaac atgtaggcct tgctggtggt
     3361 gacggtggtg gccgtggcgg gatcgttgtt gatcatgccg gtgcgggttg tttgatccgt
     3421 gacggactgg gtcacgacgg cctggcccca atggatggcg taagtgttga cggcgtcgct
     3481 cgtcgtctgt tgtttgacca cttcgccggc gtctttttgc ttatttccat tggcgtcgtt
     3541 gaaaacgtcg ttggtgctgg tggtgtggga ggttcccgtc gcggacacca aacgtccttg
     3601 ggaatcgtag cggtacgcca cgtgggaggt cgtttcggat tcgctcgtcc ccgtcgcgga
     3661 ccccgaggtg gtcgtggatt ccagcaattg ggcttgtccc caaatgatgc cgtagacgtt
     3721 ggtgctggag aagtccgaaa tctgatcgga ccattccggg ggatcgatcg tcgccggatc
     3781 gttgtcgggt tcgctgtgaa cgcgggtttt gttcgtcccg ctggacgagc cggtggccga
     3841 atccaattgg cccaaggcgt tgtagtggta attcatgtag gaggtcgatt ccgtggtgac
     3901 gttgccgttc accatggtcg aggtggtttt cgaccgggaa accacggctt gtccccccac
     3961 cacgatgtag tcgctcacgg tgtggctgtg attggtttgg gaggactcgc gggccacttc
     4021 gcccgccttg acggtcaagg ttttggtcgt gttgtccgtg gtcgattcgc cccgggcgcc
     4081 gatgacctgg cctttttcgt tgtagagggt gaaggtcacg gacacggaat gggtcgtgga
     4141 ggcgccggcc accgtggtgg aatccgaagt ggaggacgag acgaacgcct ggccaccgat
     4201 gatgtcgtag gtgttggtga tcaccgcgtc ggttacctgg agttccaact cgcccacgtc
     4261 atgcaccccg tcgttgttcg tgtcgttcca aacgcttgtt tcggaatggc tcgttccgtg
     4321 cccttcggcc gcgatcagtt ggcccgaggc gttgtaggag taattgacgg tgatcgtgga
     4381 cgtgctgcgc gtggctcccg attcggtcac ggtggtcgtg acggcctggg acacgacggc
     4441 ttggccccag atcgaaatgt aggtgctcga ggtgtcggaa gtcgaccggc cggttttctc
     4501 gacaaaaacg cccttttgtt ccgattcgtc caggtaggtg gtcaccgatt cggtgtggga
     4561 cgt
//
LOCUS       DAOQLS010000082         4519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9176_length_4519_cov_4.184812, whole genome shotgun sequence.
ACCESSION   DAOQLS010000082 DAOQLS010000000
VERSION     DAOQLS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9176_length_4519_cov_4.184812"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1054)
                     /locus_tag="PK362_02370"
     CDS             complement(71..1054)
                     /locus_tag="PK362_02370"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNC73719.1"
                     /translation="MSGVRFLATGAALPAKVLTNADLSKIVDTSDEWITERTGIKERR
                     IAAPDEATSDLSTRAARQALERAGLQPRDVDLIVVATCTPDHLFPSTACLVQKNLGVP
                     ASIAFDVSAACSGFIYGLACVKGLLETGLAKTALLIGADTLSRFTDWTDRGTCVLFGD
                     GAGALVLQASPQSNDLLSMHLAADGGPGDILTIPGGGSRHPLGQNGNAKEFPPTIKME
                     GREVFKHAVTRMVEAAEGALAKAGRSPADLRLLIPHQANLRIIDAVAKRVSLPDDRVF
                     RNVHKFGNMSAATTIVALDEAVQEGRVKRGDLVELIAFGAGLTWGAAVLRW"
     gene            complement(1057..2082)
                     /gene="plsX"
                     /locus_tag="PK362_02375"
     CDS             complement(1057..2082)
                     /gene="plsX"
                     /locus_tag="PK362_02375"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNC73720.1"
                     /translation="MTCRIALDAMGGDHGLPVNIAGALLARKETDHEIILVGDRDLIQ
                     QELEHHGARDAFLIQHAPTVVGMHEKPADACRSKKDSSIMVAAELVAQGKADALISAG
                     NSGATMAASLWHLRRLPGVSRPAIATLMPTLVGNAIVLDVGANVDCKPKHLLQFAVMG
                     SIYAHAILGIPSPRVGLLTIGEEEGKGNLVTVETHPLLKVSGLNYVGHVEGRDVPSGV
                     ADVFVCDGFVGNILLKFGEGLAAAVLKLIKNEVRKHPLAIFGKYLLKGAFKELLRKTD
                     PSEYGGAPLLGVNGVALISHGGADAKAIKNAVRTAGVFVEAGINRQISDRLQGTNAHI
                     IPLKVSA"
     gene            complement(2132..2338)
                     /gene="rpmF"
                     /locus_tag="PK362_02380"
     CDS             complement(2132..2338)
                     /gene="rpmF"
                     /locus_tag="PK362_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNC73721.1"
                     /translation="MANPKKKHSPARRDSRRSANFRLVMGSMSRCSNCGAARPPHRVC
                     AACGYYGKELVVAPKTKKSKGEEK"
     gene            complement(2357..2812)
                     /locus_tag="PK362_02385"
     CDS             complement(2357..2812)
                     /locus_tag="PK362_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNC73722.1"
                     /translation="MKVRIHEIQDHGSMAVEGEIPASSLPLETPDRPALVGPVRVALT
                     ADADKKEVHAWGTARGRVSMACARCLARFETDLEGAVDMSVPVSETFLVVDDEVRQSL
                     LLALPSQPLCRPACRGLCPRCGRNLNQGPCGCPAEPAPSPFDELKKLIQ"
     gene            complement(2882..3424)
                     /locus_tag="PK362_02390"
     CDS             complement(2882..3424)
                     /locus_tag="PK362_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNC73723.1"
                     /translation="MTTTPAVPQRPTPYLSFSLGGEDYALPALRIREIIEYEPITPLP
                     GAPPSVRGVINLRGSVVPVVDLAVAFGLPSTVAAASTCVVVAELEVAGEVCPLGLLAD
                     AVHRVLELSPADIESPPSFGLGARLEYLSGMGKIDGQKKFLMILDLDKILVLQDAART
                     GAPRPPAASEGPPRIDSGAS"
     gene            complement(3430..>4519)
                     /locus_tag="PK362_02395"
     CDS             complement(3430..>4519)
                     /locus_tag="PK362_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC73724.1"
                     /translation="EAVRVAKDLALGDVRPRVTAMFNDEHGRLLAAMGDMIQSTKDLV
                     QAARAIADGDLTVRVVPRSEHDVLGVALAQMVEKISRVMREIRNGAASLMNAAEQVSA
                     TSQALSQGTSEQAASVEETTASLEQMSSSITQNAENSRQMEQMALQGSRDAEQSGQAV
                     GQTVEAMKQIAQKISIIEEIAYQTNLLALNAAIEAARAGEHGRGFAVVAAEVRKLAER
                     SQGAAKEIGSLAGRSVQIAERTGRLIGELVPGIKKTTDLVQEVSAASKEQSAGVAQMN
                     RAMTQVDQVTQRNASAAEELASTAEEMSTQAEALKDQTGYFILAEAIDGGGGSPPSLE
                     AGASAAPPAPVPVDPRAVDDDEKHFTRF"
BASE COUNT          774 a         1424 c         1497 g          824 t
ORIGIN      
        1 cagtcctcgg cgtaaagatc gacggtgggc gacgccttgg ccgcccggcg cgcggcggaa
       61 accgcgcccg ctaccaacga aggaccgcgg ccccccaggt caggccggcg ccgaaagcga
      121 tcaattccac caaatccccg cgcttcaccc ggccctcctg gacggcctcg tccagggcca
      181 cgatcgtcgt ggccgcggac atgttgccga acttgtggac gttccggaac acccgatcgt
      241 ccggcaggct gacccgcttc gccacggcgt cgatgatgcg caaattggct tggtgcggga
      301 tcaacagccg caaatccgcg ggggaccgtc cggccttggc cagcgcgcct tcggcggctt
      361 ccaccatccg ggtcacggcg tgcttgaaca cttcgcggcc ttccattttg atggtgggcg
      421 gaaattcctt cgcgttgccg ttttggccca ggggatggcg ggacccgccg cccggaatcg
      481 tcaaaatatc ccccggtccg ccgtcggcgg ccagatgcat ggacaacaaa tcgttggatt
      541 ggggggaggc ctgaaggacc aaggcgccgg ccccgtcgcc aaaaagcacg caggtgcccc
      601 ggtcggtcca gtcggtgaag cgggacaaag tgtcggcccc gatcaggaga gccgttttgg
      661 ccagcccggt ctccaggagg cccttcacgc aggccaggcc gtagatgaac ccggaacagg
      721 cggccgacac gtcgaaagcg atggaggccg gaacgccgag attcttttga accagacagg
      781 cggtggaagg aaaaaggtgg tcgggggtgc aggtggcgac gacgatcagg tcgacgtccc
      841 ggggttgaag gcccgcccgt tccagggcct ggcgcgcggc ccgggtggag aggtccgagg
      901 tggcttcgtc gggggcggcg atgcgccgtt ccttgatgcc cgtccgttcg gtgatccact
      961 cgtcggaggt gtcgacgatt ttcgacaaat cggcgttcgt gagaaccttc gccgggagcg
     1021 ccgccccggt ggccaaaaac cggacgccgc tcacggttag gccgacacct tgagcggaat
     1081 gatgtgggcg ttggttccct gcaggcggtc ggaaatctgg cggttgatgc ccgcttcgac
     1141 gaacaccccc gcggtgcgca cggcgttctt gatggccttg gcgtcggcgc cgccgtggga
     1201 gatcaaggcc acgccgttaa cgcccaaaag cggcgcccct ccgtattccg aaggatcggt
     1261 tttgcgcagg agttccttga aagcgccctt gagcaggtat ttgccgaaaa tggcgagggg
     1321 gtgtttgcgg acttcgtttt tgatcaactt caggacggcg gcggccagac cttcgccgaa
     1381 tttcaaaagg atgttcccca cgaatccgtc gcaaacaaaa acgtcggcga cgccgctcgg
     1441 gacgtcgcgg ccttcgacgt ggcccacgta gttgaggccg ctgactttga ggagcggatg
     1501 ggtttccacg gtgaccaaat tgcccttgcc ctcctcctcg ccgatggtca agagccccac
     1561 ccggggagag ggaatgccca gaatcgcgtg ggcgtagatg gaccccatga cggcgaattg
     1621 aagcaggtgc ttgggtttgc aatcgacgtt ggcgccgacg tccaggacga tggcgttgcc
     1681 gaccagggtc ggcatgaggg tggcgatggc gggccgggag acgccgggaa gccggcgcag
     1741 gtgccacagg gaggccgcca tggtcgcgcc ggaatttccg gcggagatca gcgcgtcggc
     1801 cttgccctgg gccaccaact cggcggcgac catgatcgag gaatccttct tggagcggca
     1861 ggcgtcggcg ggcttttcgt gcatgccgac caccgtcggc gcgtgctgaa tgaggaaggc
     1921 gtcccgggcg ccgtggtgtt ccaattcctg ctggatcaaa tcccggtcgc cgaccaaaat
     1981 gatctcgtgg tcggtttcct tccgggccag aagggccccc gcgatgttga cgggaagacc
     2041 gtggtctccc cccatggcgt ccaaggcgat gcggcaggtc atcgacgggt caccagggcg
     2101 cgaaagaaat tcgagagcga ctcaacaatt cttatttctc ttcgcctttg gactttttcg
     2161 tcttgggggc gaccaccaat tctttgccgt aataaccgca ggccgcgcaa acgcggtggg
     2221 gcggacgggc ggccccgcag ttcgagcaac gggacatcga tcccatcacg agacggaaat
     2281 tcgcggaacg acggctgtcg cggcgcgcgg gggagtgttt tttctttggg ttggccatgg
     2341 ggttcttttc cttcgtttat tgaatcaatt ttttgagttc gtcaaaggga gacggggccg
     2401 gttcggcggg gcacccgcag gggccctggt tcaagtttcg tccgcagcgg gggcacaaac
     2461 cccggcaggc gggccggcag aggggctggg acggcaaggc cagcaacaag ctctggcgga
     2521 cttcgtcgtc caccaccaaa aaggtttcgc tgacggggac gctcatgtcg acggccccct
     2581 ccaaatcggt ttcaaatcgg gccaagcacc gggcgcaggc catggaaacg cggccccggg
     2641 cggtgcccca ggcgtgaacc tcttttttat cggcgtcggc ggtcagggcc acccggaccg
     2701 ggcccaccag cgcgggccga tcgggggttt ccaaggggag ggacgacgcc gggatttccc
     2761 cctccaccgc catggaaccg tgatcctgaa tttcgtgtat acggactttc acggctcctc
     2821 cgaaaagagc aaaaaacccc cttccggggg ttccgttgaa ttttcgtgag gtattttagc
     2881 gttaagaggc gccgctgtca atccgcggcg gaccctccga agcggcgggc gggcgggggg
     2941 cgcccgttcg ggcggcgtcc tggagaacca gaatcttgtc caaatcgaga atcatgagga
     3001 atttcttttg cccatcgatt tttcccatgc ccgaaagata ttccaagcgg gcgcccaggc
     3061 cgaaggacgg cggggattcg atgtccgcgg ggctcaactc cagcacccgg tggacggcgt
     3121 cggccaacag tcccaagggg caaacctccc cggcgacctc caattcggcg accaccacac
     3181 acgtcgacgc cgccgcgacg gtcgatggca aaccgaaggc gacggccaaa tcgaccacgg
     3241 gcacgacgct cccccgcaaa ttgatgaccc ctcgaacgga gggcggcgcc cccggcaacg
     3301 gggtgatggg ctcgtattcg atgatttctc ggatccggag ggccggcagg gcgtaatcct
     3361 ccccccccaa agaaaaggaa aggtagggcg tcgggcgttg agggacggcg ggcgtggtcg
     3421 tcacggcggc taaaaccggg tgaaatgttt ttcgtcgtcg tccaccgccc gcggatcgac
     3481 cggaaccggc gccggggggg cggccgaagc gcccgcttcc agggagggag ggctcccgcc
     3541 cccgccgtcg atggcctcgg cgaggatgaa ataaccggtt tggtccttca aagcctcggc
     3601 ctgggttgac atttcctcgg ccgtgctggc caattcttcg gcggccgagg cgttccgctg
     3661 ggtcacctga tccacctggg tcatggcccg gttcatttgg gcgaccccgg ccgattgctc
     3721 tttggacgcg gcggagactt cctggaccaa atcggtcgtt tttttaatgc ccggcaccag
     3781 ctccccgatc aatcgaccgg tgcgttccgc gatctgaacg ctgcgtcccg ccaaggaacc
     3841 aatctccttg gcggcccctt ggctccgctc cgccaacttg cggacctccg cggccacgac
     3901 ggcgaaaccg cgcccgtgct ccccggcccg ggccgcttcg atggccgcgt tcagggccag
     3961 aagattcgtt tggtaggcga tttcctcaat gatggaaatt ttttgggcga tctgtttcat
     4021 ggcctccacg gtctgcccga cggcttggcc gctttgctcg gcgtcccggg acccctggag
     4081 ggccatttgt tccatttgac ggctgttctc ggcgttctgg gtgatggacg agctcatttg
     4141 ctcgagactg gccgtggttt cctccacgct cgccgcctgc tcgctggtcc cctgggaaag
     4201 tgcctggctg gtggccgaaa cctgttcggc ggcgttcatg aggctggcgg cgccgttgcg
     4261 aatctcccgc atcacgcggc tgattttctc aaccatctgc gcgagggcca cccccagcac
     4321 gtcgtgttcc gatcggggca cgacccgcac ggtcaaatcc ccgtcggcga tggcccgggc
     4381 cgcctgcacc aggtctttcg tcgattggat catgtccccc atggcggcga gcaatcggcc
     4441 gtgttcgtcg ttgaacatcg ccgtcacccg gggacgcacg tcgcccaacg cgaggtcttt
     4501 ggcgacccgc accgcctcg
//
LOCUS       DAOQLS010000083         4497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9251_length_4497_cov_3.237055, whole genome shotgun sequence.
ACCESSION   DAOQLS010000083 DAOQLS010000000
VERSION     DAOQLS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9251_length_4497_cov_3.237055"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(356..1054)
                     /gene="cysE"
                     /locus_tag="PK362_02400"
     CDS             complement(356..1054)
                     /gene="cysE"
                     /locus_tag="PK362_02400"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNC73725.1"
                     /translation="MFQTLRRDLFAVMTRDPAARNRLEVILCYPGLHAVWIHRLTHAL
                     WRRGFKTPARFLSQVARSWTGIEIHPGAVIGPGFFIDHGMGVVIGETAEIGANVTLYQ
                     GVTLGGTSWHKGKRHPTLRDGVVVGAGAKVLGAFEIGERSRVGANAVVVKPVPPDSIV
                     VGVPGEIVRGGAHAHLHRPDLDHGRLPDVLSDAIGSLSAKLALLESRLANEKDQALLT
                     APDRAVRQDDDFSI"
     gene            complement(1054..1989)
                     /gene="cysK"
                     /locus_tag="PK362_02405"
     CDS             complement(1054..1989)
                     /gene="cysK"
                     /locus_tag="PK362_02405"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNC73726.1"
                     /translation="MKIAKNVTELIGNTPLVRLGRLAQGVGADIVLKLEFFNPAHSVK
                     DRIGLAMIEAAEAAGKIKPDTIIVEPTSGNTGIALAMVCAARGYKLVLTMPETMSKER
                     RMLLKAYGAQIILTPGPEGMGGAIKRAEDLAASDPRYFIPQQFKNVANPAVHRKTTAE
                     EIWRDTDGKADVLVSGIGTGGTITGVGEVIKARKPSFRCVAVEPDASPVLSGGAKGPH
                     PIQGIGAGFVPSILNTKIYDEVVRVKNDDAFDIARRLATEEGVLGGISSGAAVWAALQ
                     VGRRPESRGKMIVVIIPSFGERYLSTALYAHLADN"
     gene            complement(2227..2898)
                     /locus_tag="PK362_02410"
     CDS             complement(2227..2898)
                     /locus_tag="PK362_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNC73727.1"
                     /translation="MRYVDRPAAGRRLAEALGPWTDEGTLVMALPRGGVPVGHEVARA
                     LKIPLAALPARKLGAPGRPEFAVGALAGYESPAVALDNEALQSLGVTPDYVAEEIARQ
                     RARLAESVARFAPPGERPVLRGRQIILVDDGVATGATLRAALTFLEAQGAPPVVLALP
                     VGPRDTLRKLRPLVRALLCPWIPHPFYAVGDHYENFDPVPDDEVQALLRSPTVPAVPF
                     PVPFH"
     gene            complement(2895..3311)
                     /locus_tag="PK362_02415"
     CDS             complement(2895..3311)
                     /locus_tag="PK362_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73728.1"
                     /translation="MLKATLGKEIVVRRTNEIGALADIAKTVAEKGIGILAVSGWVEG
                     SEGVFRLLVDDALRAGDALRAKHYAPRESSVVIVELPHKPGLLKMMTEKLRDARIDLH
                     HLYASAPSRAAECLVVFSSSDNQRAMVELNHADPPR"
     gene            complement(3305..3511)
                     /locus_tag="PK362_02420"
     CDS             complement(3305..3511)
                     /locus_tag="PK362_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73729.1"
                     /translation="MTKTKRREPMFKGVFAPSMDMGEFLEERGDLRAVFDRVHGHFFD
                     AWTWEYPRRRPRTASKKTGGGPPC"
     gene            complement(3523..4422)
                     /locus_tag="PK362_02425"
     CDS             complement(3523..4422)
                     /locus_tag="PK362_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC73730.1"
                     /translation="MKKWTTPLTWLVGVDGSAHAERAARFALERAAVGGGTIRGVSIV
                     PPLRRFPRPGEIAVVEALALRARTALDQWADRCRARGVRVETRALESDRPAEALLKEA
                     DRARASLVVVGARGLSPFKAFLMGSVSQRVVRRSRRPVLVVRGNPPPRGLRALVAVDG
                     SSTGRRAIAFLKKIGLPAGSTVTAVHVVADPLALWAPEAGYGGGYPGGPFPLESRTEL
                     RRRGRTLLDKAVRDLRTVFPGARGRLTEGHPPVELLAEAKKARAHLVVLGARGLRGLD
                     RFFLGSVSQQVLAHAESSVLIVP"
BASE COUNT          783 a         1524 c         1502 g          688 t
ORIGIN      
        1 gaccgagccg ggggaggcga ggaaatggtt gtagcgcgct agcgcggaaa ccgcgggccc
       61 caaatcgaat tccaaccaat aaacccaagg ttcccgagtt gtgctcaccg ctctccggca
      121 gcgctatgac cgaggccggg ggccgacccc gccgcagagc tcctggggtc ggcggcgcgc
      181 cggaggcgcg acccctccgc caaggaaatg gtcggagcgt gaccgcgcca gcggaaatgc
      241 cgcgggggag cggccgctag cgcggaaacc gcgggcccta agtcggaatt ctaccaagag
      301 cgggggcggt tgggcagggg cgatgcccga aaacgggtgg ggcggataac tgtttttaaa
      361 tcgaaaaatc gtcgtcctgg cggacggcgc ggtcgggggc ggtcagcaga gcctggtctt
      421 tttcgttggc caggcgcgat tccaggagag ccaacttggc ggacagggag ccgatggcgt
      481 cgctcaacac gtcggggagg cggccgtgat ccaaatccgg ccgatggagg tgggcatggg
      541 ccccgccccg gacgatttca ccgggaaccc cgaccacgat ggaatccggc ggcaccggct
      601 tgaccaccac ggcgttggcg ccgacccggc ttcgctcccc gatttcaaaa gcccccaaaa
      661 ccttggcgcc cgcccccacc acgacgccgt cccgaagcgt ggggtgccgc ttgcccttgt
      721 gccagctggt gccgcccagg gtgacccctt gatagagcgt gacgttggcg ccgatttccg
      781 ccgtctcacc gatgacgacg cccatgccgt ggtcgatgaa aaatccgggg ccgatgaccg
      841 cccccgggtg gatttcaatg ccggtccagg accgggcgac ctgggacaaa aaccgcgccg
      901 gagttttgaa cccccgccgc cagagagcgt gggtgagccg gtggatccac accgcgtgca
      961 acccggggta gcagaggatc acttccagcc tgtttcgggc cgccgggtcc cgggtcatga
     1021 cggcgaacaa atcccggcga agggtttgaa acattaatta tccgccaaat gggcgtagag
     1081 ggcggtgctc aaataccgct cgccgaagga gggaatgatc acgacgatca ttttccctcg
     1141 actctccggc cggcggccga cttgaagggc ggcccaaacc gcggcgcccg aggagatccc
     1201 gcccaacacg ccctcctccg tggccaaccg gcgggcgatg tcgaaggcgt cgtcgttttt
     1261 gacgcgaacg acctcgtcgt aaattttcgt gttcaaaatc gacggtacaa acccggcccc
     1321 gatgccctgg atggggtgcg gccccttggc cccgccggac aggacgggcg acgcgtcggg
     1381 ctcgacggcc acgcagcgga aggagggttt gcgggccttg atcacttccc ccacgccggt
     1441 gatggtgccc ccggtgccga tgccggagac caggacgtcc gctttgccgt cggtgtcccg
     1501 ccaaatttcc tcggcggtgg ttttccggtg cacggcgggg ttggcgacgt tcttgaattg
     1561 ctggggaatg aaataccgcg ggtccgaggc ggccaaatcc tcggcgcgct tgatggcccc
     1621 gcccatgccc tcgggccccg gggtgaggat gatttgggcc ccgtaggcct tcaagagcat
     1681 ccgccgttcc ttgctcatgg tttcgggcat ggtcaaaacc agtttgtagc cccgggccgc
     1741 gcacaccatg gccagggcga tccccgtgtt gccgctggtg ggttcgacga tgatcgtgtc
     1801 cggtttgatt ttcccggcgg cctcggccgc ttcgatcatg gccaacccga tgcgatcttt
     1861 cacgctgtgg gccggattga aaaattccag tttcaaaacg atgtccgcgc ccacgccctg
     1921 ggccagtcga cccaagcgga cgaggggggt gttgccgatc aactccgtga cgtttttcgc
     1981 gattttcatt cgattctcct tgggccgggt cccggcgctc cgccggcggg ttccgccggg
     2041 gaacggtctt cccgggagac ggagaggacc cgcctccatc aaaaatccaa cgaaccggac
     2101 ggagtgatta aaggaagacg ccgaccaacc gacgatcggg gcggaacggg gcgctccgcc
     2161 gaacctttcg tcgatcgtaa aaaaatcccc ccccggcgtc aagggaaggg accgaatcgt
     2221 tgccgatcag tggaaaggga cggggaaagg aacggcggga actgtcgggg accgaagaag
     2281 ggcttgaact tcgtcgtcgg gcacgggatc gaaattttcg taatggtccc ccaccgcgta
     2341 gaaagggtgc gggatccaag ggcagagaag ggcccgcacc agggggcgga gtttccgaag
     2401 ggtgtcccga ggccccacgg gcaacgcgag gacgaccggc ggagctccct gggcctccaa
     2461 aaaagtaagg gcggcccgga gggtggcccc cgtcgccacg ccgtcgtcca ccagaataat
     2521 ctgacggccg cgaagcacgg gccgctcccc gggcggggcg aaccgggcga cggattcggc
     2581 caaccgcgcc cgttggcggg cgatttcctc ggccacgtag tccggcgtga ccccgaggct
     2641 ttgcagggcc tcgttgtcca gggcgaccgc gggggattcg tatcccgcca gggcgcccac
     2701 cgcgaactcc ggccgtcccg gcgcccccaa tttgcgcgcg ggcagggcgg ccagggggat
     2761 cttcaacgcc cgggccactt cgtgcccgac gggaacgccc ccccggggca gggccatgac
     2821 cagggtgcct tcgtcggtcc agggccccag ggcctcggcc agacggcgcc cggcggcggg
     2881 gcgatcgacg tatctcaccg gggcgggtcc gcgtggttca attccaccat ggcccgctgg
     2941 ttgtccgagc tggaaaagac gaccaggcac tcggccgccc gggaaggcgc cgacgcgtag
     3001 agatggtgga ggtcgatccg ggcgtcccgc agtttttccg tcatcatttt caacaggccc
     3061 ggcttgtggg gcagctcgac gatcaccacc gaggattccc ggggcgcgta atgtttggcg
     3121 cgcagagcgt ccccggcccg cagggcgtcg tccaccagga ggcggaaaac cccctcggac
     3181 ccttccaccc aacccgacac ggccaggatt ccaatgcctt tttcggcgac ggttttcgcg
     3241 atgtccgcca gggcgccgat ttcgttggtt cggcgaacaa cgatttcttt tcccaaggtg
     3301 gctttcagca cggcgggcct cctccggttt ttttggacgc cgtccgcggg cggcggcgcg
     3361 ggtattccca ggtccaggcg tcgaaaaagt gcccgtgaac ccggtcaaag accgcgcgca
     3421 aatccccccg ctcctccagg aattccccca tgtccatgga cggggcgaac acccccttga
     3481 acatcggctc tcggcgcttg gtttttgtca cgggacctcc tcttacggca cgatcaacac
     3541 cgacgactcc gcgtgggcca gcacctgctg ggaaacgctg cccagaaaaa accggtcgag
     3601 cccccgcagg ccccgggccc ccaggacgac gaggtgcgcc cgggcctttt tggcttcggc
     3661 caacaattcg acgggcggat gcccctcggt caaacggccc cgggcgccgg ggaataccgt
     3721 gcgaagatcc cgtacggctt tatcgaggag cgtccggcct cggcgccgaa gttcggttcg
     3781 ggattccagg ggaaaagggc ccccggggta tccgccgccg tagccggctt cgggcgccca
     3841 gagggccaat ggatcggcca ccacatggac cgccgtgacc gtcgaaccgg cgggcaaccc
     3901 gatctttttc aaaaaggcga tggcgcggcg tcccgttgac gaaccgtcca cggccacgag
     3961 cgcccgaagc ccccgggggg gcggatttcc gcgaaccacc agcaccggcc gccgggaacg
     4021 gcgaaccacc cgctgggaaa cgctgcccat caaaaacgcc ttgaacggcg aaagcccccg
     4081 ggccccgacc accaccagcg aggcccgcgc ccgatccgcc tccttgagca gcgcctccgc
     4141 cggacgatcg gattccagcg cccgggtctc gacccggacc ccccgggcgc ggcaacggtc
     4201 ggcccattgg tcgagggccg tgcgggcccg aagggccagg gcttcgacga cggcgatttc
     4261 ccccggccgg gggaaccgcc ggaggggcgg cacgatggag acgccccgga tcgtcccacc
     4321 gccgacggcg gcccgctcca aggcgaaccg cgccgcccgc tcggcgtggg cggagccgtc
     4381 cacgccgacc agccacgtca aaggcgttgt ccactttttc acggcgtccc cctcaccaca
     4441 tctcccgaac cgtcccgccc gccgtgttcg gggaagccag gcttcgccat ccgcccc
//
LOCUS       DAOQLS010000084         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9305_length_4483_cov_4.713415, whole genome shotgun sequence.
ACCESSION   DAOQLS010000084 DAOQLS010000000
VERSION     DAOQLS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9305_length_4483_cov_4.713415"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK362_02430"
     CDS             complement(<1..193)
                     /locus_tag="PK362_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HNC73731.1"
                     /translation="MKAVLALETGRVFEGRAFGAAGDTEGEVVFCTALTGYQEILTDP
                     SYQRQIVVMTYPHIGNYGTF"
     gene            complement(190..1479)
                     /locus_tag="PK362_02435"
     CDS             complement(190..1479)
                     /locus_tag="PK362_02435"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNC73732.1"
                     /translation="MSTLIVVGAQWGDEGKGKIVHLLGKKADLIVRYQGGNNAGHTVV
                     FDGKHFVLHQIPSGILQPGKTCLIANGVVMDPWALREEAQFLESRKIRVAGRLFISAW
                     AHLILPYHRYLDALRESGSGKIGTTKRGIGPAYSDKVGRVGIRLADFMDRQAFHDLVE
                     QNLQAKAPLLTSLVSLDKLREETYKDEEALRAFFKPLVADTPALLQKALQKKKNILFE
                     GAQGTLLDVDFGTYPFVTSSNPIAGAACVGSGVGPASIDEVLGVVKAYTTRVGDGPFA
                     TELNDDFGKSLRERGQEFGATTGRPRRCGWFDAVVVRHAVRINGLTRLSLTKLDVLEG
                     VDPIRVCVAYKIDGKIVKDFPASRREQQIAEPVYKNLPGFRGPVKGITRYEKLPKTAQ
                     RYVRFLEKEVGVPMTLISMGRSREETIVMDKKFRWVP"
     gene            complement(1480..3078)
                     /locus_tag="PK362_02440"
     CDS             complement(1480..3078)
                     /locus_tag="PK362_02440"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNC73733.1"
                     /translation="MNAESAVADAAAQRLFPSPEPLVRLANAFHDPLNNAVATARTCY
                     SPRVITAEEVGRDEKSRAQRDAIAESTYKAGHHTTLQHATFQFVLERVSRQFIWSFLH
                     SHPFYNSEQVSQRYVTVGPDTLVTPPLTGRALALYRDTAARQMDAYVRLTEMLRPTVE
                     REYKKIFPLRNPAEKPWAKIVQRKAQEVARYVLPLATHAHLYHTISGLTLHRYHRICQ
                     SFDTPREQRVVIGKMIDAVRAVDPAFVERLEDPIPLDQTPEYRCFQEFHGSASPARAF
                     LREFDLRLEGLSSKMVDHKANGEAVLAQAVRSVLGVPRAVLSDDDAIDRVLNPARNPY
                     LSESLNLGGHAKLLRTMVHMHFTFAKKISHTADSQDQRHRMTPASRPVLSGHFVPDAP
                     DFITPELIRHSPEAQAFYEGNLTALWGKINDLLNLGVSREDALYLLPNAVSVRFEESG
                     DLLNFHHKWTKRLCYTAQEEIWRTSKEEVDQLARVAPRVARHLGAPCALRASAGVRPF
                     CPEGDRFCGVLVWKKPVSAYERIL"
     gene            3255..3785
                     /locus_tag="PK362_02445"
     CDS             3255..3785
                     /locus_tag="PK362_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC73734.1"
                     /translation="MGTLAALLAAPLWVAAAAPSAPPEDSTVVDSGSVKSPVQPATPQ
                     DYAHFVASFDFKKHTATLREFLKWRAEKNVVVADLRTREDFDRGHVAGAVYLGSDITQ
                     ERLAALLPDPQTRLLVYCANSFFPTRRLALTDVALPQIAFLGHPRVWMLEAVWRDPGN
                     KSRSIPWEGNDPPHPW"
     gene            complement(3819..4355)
                     /locus_tag="PK362_02450"
     CDS             complement(3819..4355)
                     /locus_tag="PK362_02450"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNC73735.1"
                     /translation="MADMRAIPWVFSWTQSRNLLPAWYGAGSALQEFLDEKGAAGRGL
                     LADMYARWPFFAALLDNLELSLAKADLTIAEGYAALVKDRSLRSAIFGRVREEHRRTV
                     GALRAITGHASPLEQSPVLRESIALRNPYVDSLSALQTRFLSLWRDPRRSPAERDKAL
                     GVLLVTINGIAAGMKSTG"
BASE COUNT          783 a         1395 c         1474 g          831 t
ORIGIN      
        1 cgaacgtccc gtagttgccg atgtggggat aggtcatcac cacgatctgc cgttggtagg
       61 aggggtcggt caggatttcc tgataccccg tcaacgccgt gcaaaagacc acttctccct
      121 cggtgtcccc ggccgcgccg aaagcgcggc cttcgaaaac gcggcccgtt tcgagggcaa
      181 ggacggcctt cacgggaccc accggaattt tttgtccatc acgatggtct cctcccgact
      241 ccgccccatg gaaatgagcg tcatgggaac gccgacttcc ttctcgagaa accgaacgta
      301 gcgctgcgcc gttttcggca atttttcgta gcgcgtgatc cctttgaccg gcccccggaa
      361 cccgggcagg tttttgtaaa ccggctcggc gatttgttgt tcccggcggg aggccgggaa
      421 atccttgacg attttcccgt cgattttgta ggcgacgcag acgcggatgg ggtcgacccc
      481 ctccaggacg tcgagcttcg tcaacgacag gcgcgtcaac ccgttgatcc gcacggcgtg
      541 gcgcacgacc accgcgtcga accagccgca gcggcgggga cgccccgtgg tggcgccgaa
      601 ttcctgcccc cgctcccgca gggacttgcc gaaatcgtcg ttcagctccg tcgcgaaggg
      661 cccgtcgccc acccgggtgg tgtaggcttt gaccacgccc aacacctcgt cgatcgaagc
      721 cggccccacc ccggaaccca cgcaggccgc gcccgcgatg gggttggagg acgtcacgaa
      781 gggataggtc ccgaaatcca cgtccagcag ggtcccctgg gcgccttcga aaagaatgtt
      841 cttctttttt tgcagggctt tttgcaggag cgccggcgtg tcggccacca ggggtttgaa
      901 gaaggcccgc agggcttcct cgtccttgta ggtttcctcc cgcagcttgt ccagggacac
      961 caaactggtc agcagggggg ccttggcctg gagattctgc tccacgagat cgtggaaggc
     1021 ttgccggtcc atgaaatccg ccaggcggat gcccacccgc ccgaccttgt cggaataggc
     1081 ggggccgatg ccgcgcttgg tcgtgccgat cttcccgctg ccggattccc gaagggcgtc
     1141 gaggtaacgg tggtagggca aaatcagatg ggcccaggcg ctgatgaaca accgacccgc
     1201 cacccgaatc ttgcgggact ccaggaattg cgcttcttcc cggagggccc aggggtccat
     1261 gacgacgccg ttggcgatca gacaggtttt gccgggctgg agaatgcccg acgggatctg
     1321 gtgaaggacg aaatgttttc cgtcgaaaac aaccgtgtgg ccggcgttgt tgccgccctg
     1381 gtagcgcacg atgagatcgg cttttttgcc gagcaggtga acgattttcc cttttccttc
     1441 gtcgccccat tgggcgccga caacgatgag cgtggacatt tacagaatcc tttcgtaggc
     1501 cgagacgggc tttttccaaa cgagcacgcc gcaaaaccgg tcgccctcgg ggcagaaggg
     1561 gcggaccccg gccgacgcgc ggagcgcgca gggggcgccc aggtgccgcg cgacgcgcgg
     1621 ggccacccgg gccaactgat ccacttcttc cttggacgtc cgccagatct cttcctgggc
     1681 ggtgtagcac aaccgtttgg tccatttgtg gtgaaaattc aacaaatcgc cggattcctc
     1741 gaagcggacg ctgacggcgt tcggcaacag gtagagggcg tcctcccggg aaacccccag
     1801 gttcaacaga tcgttgatct tgccccagag ggcggtgagg ttcccctcgt aaaaggcctg
     1861 ggcctcgggg gaatggcgaa tcaattccgg ggtgatgaaa tccggcgcgt ccggcacgaa
     1921 gtggcccgag agcacgggcc gggaggccgg ggtcatccgg tgccgctggt cctggctgtc
     1981 cgccgtgtgg gagattttct tggcgaaggt gaaatgcatg tggaccatcg tccgcaagag
     2041 tttcgcgtgc ccccccaggt tcagggattc ggacaaatag ggattccggg cgggattcaa
     2101 cacccggtcg atggcgtcgt cgtcggacaa gaccgcccgg gggacgccca ggaccgagcg
     2161 gaccgcctgg gccaggacgg cctccccgtt ggctttgtgg tccaccattt tggagctgag
     2221 cccttccaat cgaaggtcga attcacggag gaaggcccgg gccggcgacg ccgacccgtg
     2281 gaactcctgg aaacaccggt actccggcgt ttggtccagg ggaatcgggt cttccaggcg
     2341 ctcgacaaag gcggggtcca cggcgcgcac ggcgtcgatc atctttccga tcacgacccg
     2401 ctgttcccgg ggcgtgtcga agctttggca aattcggtga taccgatgga gcgtcaatcc
     2461 gctgatcgtg tggtaaagat gggcgtgggt cgccaggggc agaacgtagc gggccacctc
     2521 ctgggctttg cgttgaacga ttttggccca gggcttttcc gccgggttcc gaaggggaaa
     2581 gatttttttg tactcccgct ccaccgtcgg ccggagcatt tcggtcaacc gcacgtaggc
     2641 gtccatttgc cgggcggcgg tgtcgcggta aagggccagg gcccggcccg tcaggggcgg
     2701 ggtgaccagg gtgtcgggcc cgaccgtgac gtaacgctgg ctcacctgct cggaattata
     2761 aaaagggtgg ctgtgcagga acgaccaaat gaactgccgg gagacccgct ccaggacgaa
     2821 ttgaaaggtg gcgtgctgca gggtggtgtg gtgcccggct ttgtacgtgc tctcggcgat
     2881 ggcgtcccgc tgggcccggg atttttcgtc ccggcccact tcctcggcgg tgatgacccg
     2941 gggggaataa caggtccggg cggtggccac ggcgttgttc agggggtcgt gaaacgcgtt
     3001 ggccagccga accaggggtt cgggcgaggg gaaaagcctt tgggcggcgg cgtcggccac
     3061 ggcagactcg gcgttcacgg gttttgttcc tatgtgaccg cccggagcgg gcggagggtg
     3121 gggaccgacg gttgtcggac ccgcaccagt ctaccaaagg cgggaagggc cgtcaaacca
     3181 atcccgggcg gggggctttc tttgaagatc ggccgaaaag ggtgtatgat tttcccatgg
     3241 gacaccgggg atcaatggga actctggccg cgttgctggc ggcgccgctg tgggttgcgg
     3301 cggccgcccc ctccgccccc ccggaagact cgaccgtcgt cgattccggg agcgtcaaat
     3361 cgcccgtcca gccggccacg ccccaggatt acgcccattt cgtggcgtcc ttcgatttca
     3421 aaaaacacac ggccacgcta agggaatttc tcaaatggcg ggccgaaaaa aacgtcgtcg
     3481 tggcggacct tcgaacccgg gaggattttg atcgggggca cgtcgcgggc gccgtttatt
     3541 tgggttcgga catcacccag gaacgattgg ccgccctgtt gccggacccc caaacacgcc
     3601 tgctcgttta ttgcgccaat tcctttttcc ccacccgtcg cctcgcgctg acggacgtgg
     3661 ccttgcccca gatcgctttc ctcggacacc cccgggtgtg gatgctcgaa gcggtgtggc
     3721 gggacccggg aaacaaatcc cgatccatcc cctgggaagg caacgaccct ccccacccct
     3781 ggtagttcgc cggcccgttc tcatcgggcc gatttcgcct atcccgtgct tttcatcccc
     3841 gccgcgatgc cgttgatggt gaccagaagc acccccaggg ccttgtcccg ctcggcgggg
     3901 gaccggcggg ggtcccgcca caaacttaaa aatcgcgtct gaagggcgct cagggaatcg
     3961 acgtagggat ttcgcagcgc gatggattcc cgaaggaccg gggattgttc caggggcgaa
     4021 gcgtggcccg tgatcgcccg cagggccccc accgtgcgcc ggtgctcctc ccgaacgcgt
     4081 ccaaagatcg ccgatcgcag ggaccgatct ttgacgaggg cggcgtaccc ttccgcgatg
     4141 gtcaaatcgg ccttggccag ggaaagctcc aaattgtcga gcagcgccgc gaaaaagggc
     4201 caccgggcgt acatgtcggc caacagcccg cgccccgcgg cgcccttttc gtccaggaac
     4261 tcctgaaggg cgctccccgc cccgtaccag gcgggaagta aatttcgcga ctgggtccag
     4321 gaaaaaaccc aggggatggc gcgcatgtcc gccagggacg gcgccgccgg ggcccggggg
     4381 gacccgtctt tcggttttcc gcttcgaaaa ccggtcggct ggcgatttcc actcgctcga
     4441 tcaggtcaat gggcgtggcc tgggcgaagt attcggcgaa acc
//
LOCUS       DAOQLS010000085         4476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9330_length_4476_cov_4.637186, whole genome shotgun sequence.
ACCESSION   DAOQLS010000085 DAOQLS010000000
VERSION     DAOQLS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9330_length_4476_cov_4.637186"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..493)
                     /locus_tag="PK362_02455"
     CDS             complement(116..493)
                     /locus_tag="PK362_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73736.1"
                     /translation="MSIRPIFPEALLKKSGLKRIPINIWDDYEDDGAIPPGKVQETWA
                     YVESSLLSTELMDDVLRVFCETLNKKHRVRGVSFALRKRREGALEIAIKGLTHAERIK
                     LVRNLKKERPTYQGLVLDVYSES"
     gene            509..1408
                     /locus_tag="PK362_02460"
     CDS             509..1408
                     /locus_tag="PK362_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC73737.1"
                     /translation="MKPRQRKIIYPVIVLFEFLIVAALWGGSYLFMRVAGPAFGVLPL
                     VGLRLSIGGAFLLGLCGLNGKFGEIRRHWKKTVLLGVSNSAVPFSLLAYTTIHTNAGF
                     GAILNSTAPFFTAVVGWVWLRERLRFSQGLGLLIGGLGVCLLVGGRLSFHSTRLAAAI
                     AAALMGTLLYGFSIVYTKKYLADVSPLAITTVSQVTGAMVLAPFVIARWPAQSPGPAA
                     WWSVGLLGVFSTAVAFVSFYRLIARLGATRTVVVTYLIPVFGMVWGGLFLGERVTVSM
                     IGSTVVILFGTALSQGVVGSRSP"
     gene            complement(1405..1857)
                     /locus_tag="PK362_02465"
     CDS             complement(1405..1857)
                     /locus_tag="PK362_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNC73738.1"
                     /translation="MRRSPPPKRTNNEWVFAAPSGIHGSGLFARAEIPAGTLLVEYDG
                     PRVSAAEGRQRAAEGNAFVFGLNRREFIDGSVGWNLGRFANHSCEPNAASESRGGRIW
                     LRALRVIFPKEEITYDYGFSFRDDPTPCRCGAPNCRGNIVAARHRDRS"
     gene            complement(1861..2139)
                     /locus_tag="PK362_02470"
     CDS             complement(1861..2139)
                     /locus_tag="PK362_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNC73739.1"
                     /translation="MKKNRIGPPADGRAAPTPPPTFFKLRVRPGSRKTSVFKKADDVY
                     EIHVRAPAERGLANAEALGLLARALDCPPNRLRLVKGGTSPHKIVQRL"
     gene            complement(2136..2675)
                     /locus_tag="PK362_02475"
     CDS             complement(2136..2675)
                     /locus_tag="PK362_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73740.1"
                     /translation="MKSTIPLLLLAASTVFPALSRAESPLLSSTYTFLAKDCRWAFNE
                     TDLEEGQDNARICRGPAPYELFIDFSAEAAFLSARRSDRPNDTVFGDPVGGIDEEKGA
                     VEWRLAGGVPFALIVRTKRRTTLDDGREWTSERLAVRGLGNHSEITGDVDVHKTPHAN
                     AAARTLADRQWRVHRADER"
     gene            2792..4360
                     /locus_tag="PK362_02480"
     CDS             2792..4360
                     /locus_tag="PK362_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73741.1"
                     /translation="MNVPKKVVLCFLFLLAGGPLWAADRPFLMGKTPISISTSVFPVW
                     SFENMDDQDLVSIHGDDFLGIPWDQFADGGPLPASWVRQWSAIAEAAIQSGKIRYLAL
                     SPLDSRKTLAQKVDAAGAKQTGWVPVDADGCYPFSTDPNAEKYKRAYIRQANYLIDLL
                     RPRFFSPAIELDIQFYSCPAQKSAYLQWYGDVHRALKAAHPNLVIFPTFQFEYLYGVA
                     LSSGAPYTPWCGGPRTDATLRACFQQHLNEALSVPGDRIAFSMYPLQFLYPPIPPDSY
                     TVGFPLAEAFSMVRQSTDRKIWVAETGWPAVKILTSYQHAAPPSACGAVLVDTSAVGG
                     ESNMGDYLTALLAQAQEKDFEAVVWWENRDLLNDADADLCPCPAGTDAFVILENFYTL
                     GGVAAELSLRRFGNMGLRRFDGSPRAAHAIWKVALARPLATPGSLDRIKAFPNPLRPG
                     GGVMTLTGLPADARLKLYSLNGRPVRTLFADFTGQAAWDGTDAGGGPVDSGVYYLHAE
                     GAGDHRTIKIVVQR"
BASE COUNT          743 a         1376 c         1380 g          977 t
ORIGIN      
        1 gaatacccca aagagggcgc gacgtcggac cggggggaag ttcaggtggg gatttttttc
       61 ttttcgaagg tgaaaggaaa gtggttcttt acccgggcga ctctggatcg ctgacctagg
      121 attcgctgta cacgtcgaga acgaggcctt ggtacgtcgg tctttctttt tttaaattcc
      181 gcaccagttt gattcgttcc gcgtgcgtca agcctttgat ggcgatttca agggcgcctt
      241 cccgccgttt ccgcagggcg aaggacaccc ctcggacgcg gtgtttttta ttgagcgtct
      301 cgcagaacac ccgaagcaca tcgtccatca attccgtcga taagagggac gattcgacat
      361 aagcccaggt ttcttgaacc ttccccgggg gaatcgcccc gtcgtcttcg taatcgtccc
      421 aaatgttgat ggggatcctt ttcaatcccg acttttttag aagcgcctct ggaaaaatcg
      481 gccggatgga catgaagcct cccgttgggt gaaaccgcgt caaaggaaaa tcatctatcc
      541 cgtgattgta ctctttgaat ttttgattgt cgccgccctt tggggcgggt cctacctgtt
      601 catgcgggtg gccggaccgg ccttcggcgt gctccctctg gtgggcctgc ggctttccat
      661 cggcggggcc tttctcctcg ggctctgcgg tctcaacggg aagttcggcg aaatccgtcg
      721 gcattggaaa aagaccgtct tgttgggcgt ttccaattcg gcggtgccat tttccctgct
      781 ggcctacacc acgattcaca ccaacgcggg gttcggcgcc attcttaatt ccaccgcccc
      841 cttttttacc gcggtggtgg ggtgggtctg gcttcgggag cggcttcggt tttcccaagg
      901 gctgggattg ttgatcggcg ggctcggggt gtgtttgttg gtgggcggac gactctcctt
      961 ccattcgacg cgattagccg ccgccatcgc ggcggcgctg atgggcaccc tgctttacgg
     1021 tttttcgatc gtgtacacga aaaaatattt ggcggacgtg agccccttgg ccattaccac
     1081 cgtcagccaa gtcacgggcg cgatggtcct ggcgcccttc gtcatcgccc gatggcccgc
     1141 ccagagcccc ggccccgcgg cctggtggag cgtggggttg ttgggcgttt tcagcacggc
     1201 cgtcgccttc gtttcttttt accggttgat cgcccgtttg ggggcgaccc ggacggtggt
     1261 ggtcacttat ctgattcccg tttttgggat ggtgtgggga gggctgtttt tgggggagcg
     1321 ggtcacggtt tccatgattg gctcgaccgt tgtcatactt ttcgggacgg ccctcagcca
     1381 aggagtggtt gggtcgaggt ccccttagga ccgatcccga tgccgggcgg ccacgatgtt
     1441 cccccgacag ttcggggcgc cgcaacggca gggggtcggg tcgtcccgaa aagaaaaccc
     1501 gtagtcgtag gtgatttcct ctttcgggaa aatgacccga agggcccgaa gccagatccg
     1561 accgccccgg ctttcgctgg ccgcgttggg ttcgcaggaa tggttggcga accgtcccag
     1621 gttccatccc accgacccgt cgatgaactc ccgccggttg agcccgaaga cgaaggcgtt
     1681 cccttccgcc gccctttgtc ggccctccgc cgcggacacc cgggggccgt cgtactccac
     1741 caacaaggtt ccagcgggga tttccgcccg cgcgaaaagc ccgcttccgt gaattcccga
     1801 aggggcggcg aacacccatt cgttgttggt tcgtttgggg ggcggggagc gccgcatcgg
     1861 ctaaaggcgt tgaacgattt tgtgggggga agtcccgcct ttgaccagcc gaaggcggtt
     1921 cggcgggcag tccagcgccc gggccaacaa ccccaacgct tccgcgttgg cgaggccccg
     1981 ttcggcgggg gcgcggacat ggatttcata gacgtcgtcg gcttttttaa acaccgaggt
     2041 ttttctggaa cccgggcgga cccggagctt gaagaaggtc ggcggcgggg tcggcgcggc
     2101 tcttccgtcc gccggaggcc cgattcgatt ttttttcacc gttcgtcggc ccgatggaca
     2161 cgccattgtc ggtcggccaa ggtccgggcc gcggcgttgg cgtggggggt tttgtgtacg
     2221 tccacgtccc ccgtgatctc cgaatgattg cccagacccc ggaccgccaa acgttcgctg
     2281 gtccattccc gaccgtcgtc cagcgtggtt cgtcgtttcg ttcgcacgat cagcgcgaag
     2341 ggaacccccc cggccaaccg ccattccacc gcgcctttct cctcatcgat tccgccgacc
     2401 gggtcgccga aaaccgtatc gttcggccgg tcggaccgcc gggcggacag gaacgcggcc
     2461 tcggccgaga aatcgatgaa gagctcgtag ggcgccggac cccggcagat ccgggcgttg
     2521 tcctggccct cttcgagatc ggtctcgtta aaagcccacc ggcagtcctt ggccaaaaaa
     2581 gtatacgtgc ttgaaagaag gggggattcg gcgcgggaaa gggccgggaa gaccgtcgag
     2641 gcagccagga gaaggagagg aatagtcgat ttcatgagtt cgtccgtcga acgaggttga
     2701 ccgtagtccc gccgattcga agacgctata atcgttgggg ctcgaccggt ccgcagtcgt
     2761 ccttggaaca ccgcgaggga atgatatcta aatgaacgtc ccaaaaaaag ttgtcctttg
     2821 tttcctcttc ttgttggcgg gggggcctct ttgggccgcc gaccgccctt tcttgatggg
     2881 gaaaacgccc atctccattt ccaccagcgt ttttcccgtt tggtccttcg agaacatgga
     2941 cgatcaggat ctcgtttcga ttcacggcga cgatttcctg ggaatcccct gggatcaatt
     3001 cgccgacggc ggtccccttc cggcgtcctg ggtccggcaa tggtccgcca tcgccgaagc
     3061 ggccatacaa tcgggaaaaa tccgctacct cgccctgtcg cccctcgaca gtcgaaagac
     3121 tctggcgcaa aaggtggacg ccgccggcgc caaacagacg ggatgggtcc ccgtggatgc
     3181 cgacggttgc tacccctttt caacggatcc caacgccgaa aaatacaagc gggcctacat
     3241 ccgccaggcg aactatctca tcgacctgct gcggccccgg ttttttagcc ccgcgatcga
     3301 attggatatc cagttttatt cttgtcccgc ccagaagtcc gcctacctcc aatggtacgg
     3361 ggacgttcac cgcgccctga aggccgccca tccgaatttg gtcatttttc caacgtttca
     3421 atttgaatat ctgtatgggg tggccctttc gtcgggagcg ccgtacaccc cctggtgcgg
     3481 ggggccccgc acggacgcca ccttgcgcgc ctgttttcag caacatctga acgaagccct
     3541 gtccgttccc ggcgaccgaa tcgctttttc gatgtaccct cttcaattcc tctatccccc
     3601 catcccgccg gattcctaca ccgtggggtt tcccctggcc gaggcgtttt cgatggtccg
     3661 gcaatccacc gaccgaaaaa tctgggtcgc cgagaccggg tggccggcgg tgaaaatcct
     3721 cacgagctac cagcacgcgg cgccgccgtc cgcctgcggg gccgtcttgg tcgacacgag
     3781 cgcggtgggg ggggagtcga acatggggga ttacttgacc gcgttgctgg cccaggccca
     3841 ggaaaaagat tttgaggcgg tggtctggtg ggaaaatcgg gacttgctga acgacgcgga
     3901 cgcggacctc tgtccctgcc ccgccggcac cgacgccttc gtcattctcg agaactttta
     3961 caccttggga ggggtcgccg ccgaattgag cttgcgccgg ttcgggaaca tggggctccg
     4021 acgattcgac ggcagccccc gggcggccca cgcgatttgg aaggtcgcct tggcccgtcc
     4081 gttggccacg ccgggatcct tggatcggat caaggccttc cccaacccgc ttcggccggg
     4141 gggcggggtc atgaccttga cggggctccc cgccgacgcc cggcttaaac tctattcgct
     4201 gaacggccgg ccggtccgaa cccttttcgc ggacttcacc ggtcaagccg cctgggacgg
     4261 gaccgacgcc ggcggcggtc ccgtcgacag cggggtctac tacctccacg ccgagggcgc
     4321 gggggaccac cggacgatca aaatcgtcgt tcaacgctga ggacgggtct ctcgccggac
     4381 cccattgagt tgtcccgtcc ggggggatat cttggagcat gcgcctcgac gcgtcgtttg
     4441 tgttgtccac cctcttcggc ctcgtgggaa tgattt
//
LOCUS       DAOQLS010000086         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9365_length_4465_cov_4.275057, whole genome shotgun sequence.
ACCESSION   DAOQLS010000086 DAOQLS010000000
VERSION     DAOQLS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9365_length_4465_cov_4.275057"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PK362_02485"
     CDS             <1..1333
                     /locus_tag="PK362_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73742.1"
                     /translation="LVLERRRWVDPLQTFENGLLNGNLVDVEIDRGPLRGAVWSPADA
                     AAALRVRPLSTNRTIEQGRQGFFSDFGLSPYLLGTAGPSASEILEDVNRLTARLRSLE
                     VPEPRAWAERLAEISIKEKIDALRLLARYFRARDRRGDTFPLGSQRAPRTRALSDFVV
                     GLTRSVGREAGQLAALRAGAVRPGAPEALLRARTQSALERFPSLARTLLSLLEEAGVT
                     PVPGKEADRSAFAADLLRVFGHAFDEGYGRLTVLTTAAAARQAPTAKGLAVGVFHVRD
                     SLRDSLAAGELVRSLLDHARQGTLAQFAPVVLLDGPGFEHATARQLSSELQYRLAEEA
                     PGAPADLRDAALRTLAERLGVAHAEAVSQDGRVSLHRLLSWTRSQWAGQTVGTVQIFT
                     RDRDRMLDDVRGRLVDWIIYLIEPMGLRRLSSSLEDAAAEDRLRRYVASNA"
     gene            1384..2457
                     /locus_tag="PK362_02490"
     CDS             1384..2457
                     /locus_tag="PK362_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HNC73743.1"
                     /translation="MTTHNVTLIPGDGTGPEISATVQKVIDATGVKIAWDVVEAGADV
                     VEKYGTPLPENVLASIRKNKVALKGPLTTPIGTGFRSVNVALRKELDLYACLRPCKSY
                     KGVRSRYENIDLVVVRENTEDLYAGVEYETGSKEAKEIIGWAKGKIFPDAAISIKPIS
                     RTGTERIVKFAFDYAVAHKRRKVTAVTKANIMKYTDGLFFKVAQDVAKQYPGIEYEER
                     LIDNMCMQLVQKPELYDVICLPNLYGDILSDLCAGLIGGLGIAPGANHGKDIALFEAV
                     HGSAPKYKGMNKVNPTAVLLSGVLMLEHLGEKAAAQKLEAAIAAVIAEGKSVTYDLKP
                     QPTDPTAVGTSQMGDAIIKKIRG"
     gene            2497..3417
                     /locus_tag="PK362_02495"
     CDS             2497..3417
                     /locus_tag="PK362_02495"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC73744.1"
                     /translation="MNRPDRIPGSTSLLVLALASGMTLRAAEGSSTAGFLNLPSSSRL
                     AGMGEAGLALVEGSQGLGVNPASLGRLKGQGVGLTHGLYVDESSLEDLTYARATSLGG
                     FGVGVRYFSAGKIDELDTAGATVGSFTPSDLALTAGYGRALKYVKVGGAVKYIRSKLV
                     DSANALTVDLGIESNPLLRKKLRLALVGQNLIGSLKYETEKSDLPRILRAGGTYSVKG
                     QWDVAVDLGFPKDGDTEWSLGTEYRLKMAEPWGVALRTGYNSRRADVSGGEGFALGLG
                     VKRNKMTVDYAYVPFGDIGSTHWISLGWAW"
     gene            3414..3884
                     /gene="ndk"
                     /locus_tag="PK362_02500"
     CDS             3414..3884
                     /gene="ndk"
                     /locus_tag="PK362_02500"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNC73745.1"
                     /translation="MSAELSCVIVKPDGVGQALVGKILARLEEEGFRLLALRMVRPKV
                     ETMAEFYGEHRGKPFYDGLMKFMTSGPFVVTAWTGENAIARIRALVGATDPKKAAAGT
                     LRATWGTDHRRNLIHASDSPASGQRETALFFTPAELAPYDPQRWAAPDMAPPVQ"
     gene            3881..4294
                     /locus_tag="PK362_02505"
     CDS             3881..4294
                     /locus_tag="PK362_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73746.1"
                     /translation="MKMRVHGSLWALTLLGLAQGMGPSTSSAFYATAENVMVVRIVES
                     KLMQFSTKVDRYVFSVRVEVIGDCGDYPEFKKGASLTISRLEAVEGWHERIRTGVCLR
                     IVRPLEMVPRKGWKAGNWRVADVYAEDPGGCPPKP"
     gene            complement(4299..4451)
                     /locus_tag="PK362_02510"
     CDS             complement(4299..4451)
                     /locus_tag="PK362_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNC73747.1"
                     /translation="MEVLEFSDNVLTVHYQGACGSCASATSGTLYAIQNLLQRMVDPK
                     IQVVSV"
BASE COUNT          816 a         1381 c         1449 g          819 t
ORIGIN      
        1 attggtgctc gagcgtcgcc gctgggtcga cccccttcaa acctttgaga acggtttgct
       61 gaacggaaat ttggtcgacg tggaaatcga tcgcggtccc ctgcggggcg cggtctggtc
      121 ccccgccgac gcggccgccg cccttcgggt tcggccgctg tcgacgaatc gaacgatcga
      181 acagggccgc caggggttct tttccgactt cgggctttcg ccctatctgc tggggaccgc
      241 gggcccgtcg gcctcggaga ttttggagga cgtgaatcgc ctcacggcgc gcctccgttc
      301 cctggaggtt cccgaaccgc gggcctgggc ggaacggctg gcggaaattt ccatcaaaga
      361 aaaaatcgac gccctgcgcc tcctggcccg gtatttccgg gcgcgggatc gccggggcga
      421 cacctttccc ttgggaagcc aacgcgctcc gcggacccgg gcgctatcgg attttgtggt
      481 gggtctgacg cggtcggtgg gacgggaggc cggacaattg gccgcccttc gggcgggcgc
      541 cgttcgtccg ggagcccccg aggcccttct tcgggcccga acgcaaagcg ccttggagcg
      601 gtttccttcc ttggcgcgca cccttctgtc gttgttggag gaagccggtg tgacgcccgt
      661 gccgggcaaa gaggccgatc ggagcgcctt cgcggcggat ctccttcggg tgttcggcca
      721 cgcttttgac gaggggtacg gccgcttgac ggtactgacg accgccgccg ccgcccgcca
      781 ggcgcccacg gccaagggtt tggccgtcgg ggttttccac gtgcgcgatt ccctgcggga
      841 ttccctggcg gcgggcgaac tggtccgctc cctgctcgac cacgcgcggc aaggaacgtt
      901 ggctcagttc gcgcccgtcg tcctcttgga cgggccgggg ttcgagcacg ccaccgcccg
      961 ccaattgtcg tcggagcttc agtaccggct ggccgaggag gctcccggcg ccccggccga
     1021 ccttcgggac gcggcccttc gaaccttggc cgaacgcctg ggcgtggccc acgccgaggc
     1081 cgtttcgcag gatggccggg tgagcctcca ccggctcttg agctggacgc ggtcccaatg
     1141 ggccggtcaa acggtgggaa cggttcagat tttcacccgg gaccgggatc ggatgttgga
     1201 cgacgtccgg ggccgcctcg tggattggat catttacctg atcgaaccca tggggctccg
     1261 ccgtttgtcg tcctccctgg aagacgcggc ggccgaagac cgcctgcgcc gctacgtcgc
     1321 gtcgaacgct taagaattgg ctaaaatggt tttcatcaaa ttttttccca aaggacacca
     1381 cccatgacca cccacaacgt aaccctgatc cccggcgacg gcacggggcc cgaaatttcc
     1441 gccacggttc aaaaagtcat cgacgccacc ggcgtgaaaa tcgcctggga cgtcgttgaa
     1501 gccggggccg acgtcgttga gaaatacggc acgcccctcc ccgagaacgt cctggcctcc
     1561 atccgtaaaa acaaagtcgc gctgaaagga cccctgacca cgcccatcgg cacgggtttc
     1621 cggtccgtca acgtcgccct tcggaaggag ctggaccttt acgcctgcct ccgcccctgc
     1681 aagtcctaca agggcgtccg ctcccgctac gaaaacatcg atttggtcgt ggtccgggaa
     1741 aacaccgagg atctttacgc cggggtcgag tacgaaaccg gttcgaaaga ggccaaggag
     1801 atcatcggtt gggccaaggg caaaatcttc cccgacgcgg ccatctcgat caagcccatt
     1861 tcccgcacgg gcaccgagcg catcgtgaaa ttcgccttcg actacgccgt cgcccacaag
     1921 cggcgcaagg tgaccgcggt gaccaaggcc aacatcatga agtacaccga cgggctcttt
     1981 ttcaaagtgg cccaagacgt ggccaagcaa tacccgggca ttgaatacga ggaacgtctg
     2041 atcgacaaca tgtgcatgca gctcgtgcaa aagccggagc tgtacgacgt gatttgcctg
     2101 ccgaaccttt acggcgacat tctctccgac ctctgcgccg ggttgatcgg cggcctgggc
     2161 atcgcccccg gcgccaatca cggcaaggac atcgccttgt tcgaggcggt tcacgggtcc
     2221 gcccccaaat acaaaggcat gaacaaggtg aaccccacgg ccgtccttct ttcgggcgtt
     2281 ctgatgctgg agcacctggg cgaaaaggcc gccgcccaaa agctggaggc cgccatcgcc
     2341 gccgtgatcg ccgaaggcaa gtcggtgact tacgatctca aaccccagcc caccgatccc
     2401 accgccgtcg gcaccagcca aatgggcgac gcgattatta aaaagattcg tgggtaaaat
     2461 cgtgaagccc gctcccgggc gctctcgggg cccgcgttga atcgaccgga tcggattcca
     2521 gggagcacct ccctgctggt tctcgccttg gcctccggaa tgactcttcg ggcggcggaa
     2581 gggagttcca cggcggggtt cctcaacctt ccttcctcga gccggctggc gggaatgggg
     2641 gaggcgggat tggcgttggt ggaggggtcc cagggcctgg gggtcaatcc ggcgagcttg
     2701 ggtcggttga agggccaggg ggtgggtttg acccacggcc tctatgtgga cgaaagttcc
     2761 ctggaagacc tgacctacgc gcgggcgacg tccctgggcg gtttcggcgt gggcgttcgg
     2821 tatttttcgg cggggaagat cgacgaactg gatacggcgg gggcgacggt cgggagcttt
     2881 acgccgagcg acctggccct gaccgcgggg tatggccggg cgctgaaata cgtgaaagtg
     2941 ggtggagcgg tgaaatacat ccgatccaag ttggtggaca gcgccaatgc gttgacggtg
     3001 gacctgggaa tcgagtcgaa tccgctgctc cgaaaaaagc tgcggctggc gctggtgggg
     3061 cagaacctga ttgggagttt gaaatacgaa acggagaaga gcgacctgcc gcgaatcctc
     3121 cgggcggggg ggacctatag cgtgaagggg caatgggacg tcgcggtgga cctgggattt
     3181 cccaaggacg gcgacaccga atggtctctg gggacggagt atcgattgaa gatggccgaa
     3241 ccctggggag tcgcccttcg cacggggtac aacagccggc gggcggacgt cagcgggggg
     3301 gaggggtttg ccctgggctt gggcgttaaa cggaacaaga tgacggtgga ctacgcctac
     3361 gtgcccttcg gcgacatcgg ttccacccat tggatctccc tgggatgggc ctggtgagcg
     3421 cggagctttc ctgcgtcatc gttaaaccgg acggggtcgg ccaggccctg gtgggaaaaa
     3481 ttttggcccg actggaagag gaggggttcc ggctcctggc tcttcggatg gtgcgtccga
     3541 aggtcgaaac catggcggaa ttttacgggg aacatcgggg aaaacccttt tacgacggcc
     3601 taatgaaatt catgacctcg gggcccttcg tggtcacggc ttggacgggg gagaacgcca
     3661 ttgcccgcat tcgggccctg gtgggcgcca cggatcccaa gaaagccgcg gcgggaaccc
     3721 ttcgcgccac ctggggaacc gaccaccgcc gcaatttaat ccacgcctcg gactcccccg
     3781 ccagcggcca gcgggaaacg gctctttttt tcacccccgc cgaattggcc ccctacgacc
     3841 cccagcgatg ggccgccccg gacatggcac cgcccgtcca atgaaaatga gggttcacgg
     3901 atctctttgg gccttgaccc tccttgggtt ggcccagggg atgggcccgt caacctcctc
     3961 cgccttctac gccaccgcgg agaacgtgat ggtggtgcgc atcgtggaat cgaagttgat
     4021 gcaattctcc acgaaggtcg atcgttatgt tttttccgtg cgcgtggaag tgatcgggga
     4081 ctgcggcgat taccccgaat tcaaaaaagg ggcctcgtta accatttcgc gtttggaggc
     4141 cgttgaagga tggcacgaac gaattcgaac gggggtctgc cttcgaatcg tgcgtcctct
     4201 ggaaatggtc ccacggaaag gatggaaagc ggggaattgg cgggtcgccg acgtttacgc
     4261 cgaagacccc gggggatgcc cccccaaacc gtgatcgatt aaaccgacac cacctgaatc
     4321 ttggggtcca ccatccgttg aagcaggttt tggatggcgt acagggttcc cgaggtcgcg
     4381 ctggcgcaac ttccgcaggc cccctggtag tgcaccgtca acacgttgtc cgaaaattcc
     4441 aacacctcca accccccccc gtccc
//
LOCUS       DAOQLS010000087         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9389_length_4459_cov_4.244550, whole genome shotgun sequence.
ACCESSION   DAOQLS010000087 DAOQLS010000000
VERSION     DAOQLS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9389_length_4459_cov_4.244550"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..778)
                     /locus_tag="PK362_02515"
     CDS             complement(65..778)
                     /locus_tag="PK362_02515"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNC73748.1"
                     /translation="MKYKSRLIPIAPDGWRFVGVFGVLGALFLLFGAWFSKTVGGVLG
                     LLALFSLRFFRDGERTVPVTTDLLAPADGTVTEVSDVDGEGYGRGRVIRIFLSVFDGH
                     VQRAPLAGRVTKTHYQPGLFLDARNARAAFANESNAIEIESSKGRVVVRQIAGLIARR
                     IVCWVRPDDVVEAGERIGLIRYGSQVNLYVPLDVEITVKDGDKVSAGQTIVGRWKNAG
                     SSQRAGSADRTVEPAGVGA"
     gene            complement(795..1862)
                     /gene="ilvC"
                     /locus_tag="PK362_02520"
     CDS             complement(795..1862)
                     /gene="ilvC"
                     /locus_tag="PK362_02520"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNC73749.1"
                     /translation="MTTAVKTASPNGHSGLELKAKTFYEKDADLALLKSKKIVVLGYG
                     SQGHAQALNLKDSGCQVTVAVRKGGRGYALALKHGWVEGKNLITDNVAAVRDADWIHF
                     LLPDEAQGPVWKNEIAPNVKKGVVMSFSHGFNIRFGCVTPPKEADVVLIAPKGPGHLV
                     RRTYEEGKGTPALVAVEQDYSGNALALALAFCKGVGATRAGVIQTTFSEETETDLFGE
                     QNVLCGGVVDLIKAGFETLLEAGYQPEIAYFECLHELKLITDLVQEGGIGWMNYSISD
                     TAEYGEYTRGPRLITEATKAQMKKNLEEVRNGTFAKEYLLENIAGRPSINSFRKATAE
                     HPIEKVGEKLRAMMPWLKKKE"
     gene            complement(1905..3758)
                     /gene="ilvB"
                     /locus_tag="PK362_02525"
     CDS             complement(1905..3758)
                     /gene="ilvB"
                     /locus_tag="PK362_02525"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNC73750.1"
                     /translation="MSADLALHADAALPTGGDPMTGADMIPRVLAQEGVTTVFGYSGG
                     AILPAIDAFFRFNEKQPESKKIRYIVPANEQGAGFMASGFARSTGQVGVALVTSGPGA
                     TNTVTPIRDAMADSVPLVVLTGQVPRPAIGTDAFQEAPVFNIMMACAKHVFLVEKPEE
                     LESTLRTAFWIARTGRPGPVVVDIPKDVQVTPGHFKGAGLLPLRGYRRRVEALSDSRL
                     TEAQGRTFFQRLGEAHRPLLYVGGGVINANASAELKEFAETFNIPVVTTLMGIGALDN
                     RHPLSMHMLGMHGTPYANYAVEDCDFLFAVGARFDDRVAGKVAEFAPKASFIAHLDID
                     PAEIGKVKTPTWSHVADAKRGLRDLIDAGKKLGFKRDFSAWIKHTDATRKAHPLNYNR
                     KAEKIQPYAVLERIAEMTRGEAIVTTGVGQHQMWAAQYGRTAVPRRWLTSGSMGTMGY
                     GLPAAIGAQFANPGKLVIDVDGDGSLRMNFSEMETITTYNLPVKVLLLNNEGDGMVRQ
                     WQTLYFNKRYFAIDKNLHTLDFVKAAEAMGFPFARRVEKPADVDGVLKEFLSAPGPAF
                     LDVTIDPFAFVYPMVGPGMGYKEMVTGEWIKARPQAAAPHEVPGDVVPDLF"
     gene            complement(3777..4253)
                     /gene="ilvN"
                     /locus_tag="PK362_02530"
                     /pseudo
     CDS             complement(3777..4253)
                     /gene="ilvN"
                     /locus_tag="PK362_02530"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          829 a         1421 c         1319 g          890 t
ORIGIN      
        1 ctggggtaac cttccccccg caatcccaac agtcgggaca gtaaaccgaa acgcgacatc
       61 acgctcacgc gcccaccccc gcgggttcga ccgtccggtc ggcgctgccg gcgcgttggg
      121 acgatcccgc gtttttccaa cgaccgacga tggtttgacc cgccgacacc ttgtccccgt
      181 ctttcacggt gatctccaca tccaacggaa cgtacaaatt gacctgcgag ccgtatcgaa
      241 tcaggccgat ccgctccccg gcttccacca cgtcgtcggg gcgcacccag caaacgatgc
      301 gccgggcgat caaccccgcg atctgccgaa ccaccacccg ccccttggac gattcgatct
      361 caatggcgtt cgattcgttg gcgaaagccg cccgggcgtt ccgcgcgtcc aaaaacaggc
      421 ccggttgata atgggttttg gtcacgcgcc ccgccagggg ggctcgctga acgtgcccgt
      481 cgaaaacgga caaaaagatc cgaatcacgc gtccccggcc gtagccttcg ccgtcgacgt
      541 ccgagacctc ggtcacggtg ccgtcggccg gggccaggag gtcggtcgtc acgggaacgg
      601 tgcgctcccc gtcccgaaag aaccgaaggg agaaaagggc cagaagcccc agaaccccgc
      661 cgacggtttt ggagaaccag gccccgaaca acagaaaaag ggcccccagg acgccgaaga
      721 ccccgacgaa ccgccatcca tcgggcgcaa tgggaatcag tcgcgacttg tatttcatgc
      781 ggacgccggt ccggctattc tttctttttg agccacggca tcatggctcg aagtttttcg
      841 ccgacttttt cgatggggtg ttcggcggtc gctttgcgga agctgttgat ggacggtcgg
      901 cccgcgatgt tttccagaag gtattccttc gcgaatgtgc cgttccgaac ctcctccaaa
      961 ttcttcttca tctgggcctt ggtcgcctcc gtgatcaggc gcggcccccg ggtgtactcc
     1021 ccgtattcgg cggtgtccga aatggagtag ttcatccacc cgatgccgcc ttcctgcacg
     1081 agatcggtga tgagcttcaa ttcgtggaga cattcgaaat aagcgatttc cggctggtaa
     1141 ccggcctcga ggagggtttc aaaacccgct ttgatgaggt cgacgacgcc gccgcaaaga
     1201 acgttttgtt cgccgaagag atcggtttcg gtttcttcgg aaaaggtggt ttgaatgacc
     1261 cccgcccggg tggccccgac gcctttgcag aaggccaaag ccaaggccaa ggcgttgccc
     1321 gaataatcct gttccaccgc gaccagggcc ggcgtccctt ttccctcttc gtacgtccgg
     1381 cggaccaaat gaccgggacc tttgggggca atcaggacga catcggcttc tttcggaggg
     1441 gtgacgcacc caaagcgaat gttgaacccg tgggagaagg acatcacgac gcctttttta
     1501 acgttcgggg cgatttcgtt tttccagacg gggccctggg cttcgtccgg caggagaaag
     1561 tgaatccagt ccgcgtcccg gaccgcggcc acgttgtcgg tgatcagatt tttcccctcc
     1621 acccacccgt gcttgagggc caaagcatac ccccgcccgc ctttgcgcac cgcgaccgtc
     1681 acctggcagc cggaatcctt caggttcaag gcctgggcgt gcccctggga gccgtagccc
     1741 aagacgacga tttttttgct tttcaacaac gccaagtcgg cgtctttttc gtaaaaagtt
     1801 tttgccttca gttccaggcc ggaatggccg ttgggggaag ccgttttaac cgcggtcgtc
     1861 atgtgcgttt ctcctcaggg attaaaactt aattatatct atttttaaaa cagatccgga
     1921 accacgtccc ccggcacctc gtggggggcg gcggcctgcg gacgggcttt gatccattcg
     1981 ccggtcacca tttccttgta ccccatgccg ggacccacca tcgggtaaac gaaggcgaag
     2041 gggtcgatgg tcacgtccag gaaagccggg cccggggccg aaaggaattc cttcagcacg
     2101 ccgtccacgt cggcgggttt ctcgacgcga cgggcgaaag ggaaccccat ggcctcggcc
     2161 gctttgacga agtccaaggt gtgaagattt ttgtcgatgg cgaaatagcg cttgttgaag
     2221 tagagggtct gccattgacg caccatgccg tccccctcgt tgttgagcag gaggaccttc
     2281 acgggcagat tgtaggtcgt gatggtttcc atttcgctga aattcatccg gaggctgccg
     2341 tcgccgtcca catcgatcac gagcttcccg ggattggcga attgggcccc gatggccgcc
     2401 ggcagtccgt agcccatggt gcccatggac cccgaggtca accagcggcg cggcacggcg
     2461 gtgcggccgt attgcgccgc ccacatttga tgctggccca cgcccgtggt gacgatggct
     2521 tcgccccggg tcatctcggc gattctttcc agaaccgcgt agggctggat tttttcggcc
     2581 ttgcggttgt aattcaacgg gtgggccttc cgggtcgcgt cggtgtgttt gatccaggcc
     2641 gaaaaatccc gcttaaaccc gagtttcttt ccggcgtcga tcaaatcccg caaaccgcgc
     2701 ttggcgtcgg ccacgtggga ccaggtgggc gtcttgactt ttccgatctc cgccgggtcg
     2761 atgtccaaat gcgcgatgaa ggacgccttg ggcgcgaact ccgccacctt gcccgccacc
     2821 cgatcgtcga aacgcgcccc gacggcgaaa aggaaatcgc aatcctccac cgcgtagttg
     2881 gcgtagggcg tgccgtgcat gcccagcatg tgcatcgaga gcggatgccg gttgtccagg
     2941 gcgccgatgc ccatgagggt tgtcaccacg gggatgttga aggtttccgc gaactccttg
     3001 agctcggcgg aggcgttggc gttgatgacc ccgccgccca cgtagagcag ggggcggtgc
     3061 gcttccccca accgctggaa aaaggttctt ccctgcgcct ccgtcaggcg ggagtccgaa
     3121 agcgcctcca cccgacggcg gtagccgcgc aggggcagaa ggccggcgcc tttgaaatgg
     3181 cccggggtca cctggacgtc cttgggaatg tccaccacca caggacccgg ccggccggtg
     3241 cgggcgatcc aaaacgccgt gcgcagagtg ctttccaatt cctcgggctt ctccaccaaa
     3301 aacacgtgtt tggcacaggc catcatgatg ttgaacaccg gcgcttcctg aaaggcgtcg
     3361 gttccgatgg cgggccgagg cacttgaccg gtcaatacca ccaagggcac ggaatccgcc
     3421 atggcgtccc gaatgggcgt gacggtgttc gtggccccgg gtcccgaggt caccaaagcc
     3481 acccccacct gtccggtgga gcgggcgaat cccgaggcca tgaaccccgc gccttgttcg
     3541 ttcgcgggca cgatgtaacg aatttttttc gattccggtt gcttctcgtt gaaccggaaa
     3601 aaagcgtcga tggcgggcag gatggcgccg ccggaataac cgaacaccgt ggtcacgcct
     3661 tcctgggcca acacccgggg aatcatgtcc gccccggtca tggggtcgcc cccggtgggc
     3721 aacgcggcgt ccgcgtgaag ggccagatcc gccgacatgt cgagggtttt tccccgttgc
     3781 aacaacaccc ggccggttcg cgaaacttcc tgaatgccga agggctccag agccttcagg
     3841 agcgcatcca cctcgccggc gcgaccggcg ttttcaatga caaatcccgc ggggctgtcg
     3901 tgtacccggg cgtgaaacgc ttggcaggcc tggcccaccc cggatcggtc gccggatccg
     3961 attttcacca ggaccaattc gctttccacg ttgtcttccc agctcaaatc cgacactcgc
     4021 accacgtcca ccaacttgtt gagttggctg atgatttgcc cgatcacctt gtcgtcccct
     4081 ttgcaaacca gcgagattcg ggatatcccg ggaatttgag ttccgcccac ggccagggag
     4141 tcgatgttga acccccggct ggaaatcaac cccgcgatgc gggccagtac gcccgggcga
     4201 tcctccacca ggcaggaaag aaaatgcgtg tgttccgtcg gtttgtgcgt cattggtttt
     4261 tgccccaagt gtttgtcgtt ttcatcgtcc accgtttctc gatcgcctta tcgcgccgac
     4321 cgcgccgaaa caaaaacgcc gccggagctt gggcgccggc ggcggtcgtt ttaaactttt
     4381 acttaccgcc tacggcgcaa ccgcccctcc caggaggacc tggctgagaa cgacgaccgt
     4441 aagcgcgaga ccttgcaac
//
LOCUS       DAOQLS010000088         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9429_length_4447_cov_3.088115, whole genome shotgun sequence.
ACCESSION   DAOQLS010000088 DAOQLS010000000
VERSION     DAOQLS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9429_length_4447_cov_3.088115"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK362_02535"
     CDS             <1..357
                     /locus_tag="PK362_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73751.1"
                     /translation="TGSEYLQAKIERQKAFNDRNQARRNVEIDVREAYYNIQKGKIQI
                     KGAEAELDYREKELDIARQKERMNLIEPAQSLAAESSYGDAVANHEEALSFYQVSLAG
                     LERAVGMPLESIPEFR"
     gene            397..3690
                     /locus_tag="PK362_02540"
     CDS             397..3690
                     /locus_tag="PK362_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC73752.1"
                     /translation="MAGFVARRPVTGTMFYMGVALMGVISWFFTARELFPSISFPQLL
                     VITRYGNAAPEEIENLITKVIEESVGTVPNLKRVRSLSKEGVSLVTLEFDWGTDMGFS
                     HLTAREKIDQIKDRLPSEAEEPIINRINPFAQPMMIYSVSGSLPIPRLTEIAKQVIKQ
                     RLEKVTGVASASISGGQEREILVEVDRPRMEAQGISLASIVDSLKDANLNYPAGTVQG
                     KFYEYLVRTMGEFKNIAEIGRTVIKVERPQDEEERDRYGNNRDRENHPREARLSYLND
                     LASVQDTFKERTSFSRYNGHENISITIQKQADANALTTAQRVRVATEELQTILKPKGV
                     NVELVYDESRFIEDAISGVATDGIVGGILAFFVLYFFLKSWTVAAIVALAIPASTLIT
                     FTGMFFKKISINMLSLAGLGLGIGNMVDNSIVVAENIARHQTELGKGRIEAAVDGTDE
                     VAPSMVTSSLTNVAVLLPLLFAKGVAQLVFRDMFFIVTCASFASVFISITLVPLLSAH
                     PVSFAFIKTMLGIRAAEPVPGGEIPPPEAPPAKPFLDRFPLPVQKALRWFLDGFSDEA
                     YNRLMARYKSLLEWVLKNPSKTVVGITGGALLSLGLLGFQDRVFMPKVDQGQFILKIQ
                     MPVGTRLEITNEVTEKVEAVLKSLPGFREVTVSVGSNNVEAVDALGDHQSQAIVNLDR
                     SKVATEDYIETLKDTLDKQDLQGAEVQYVLQDSLLASAFETAAPIVIECKGPDMTTLR
                     KIADDILRDVTAVPGVFGAKTSLALPSSETRVEIDKLRAASYHLNTAEIARTALIGIK
                     GFVATTYKEAGQEVDVRVRLRPEDRADVDSIGRLTVLAPGNVMVPLSEIASLVSARGP
                     SEIKHLDQQRSIVVSANVAKRSTNDVIKDVTALLEKYHNLSDYQVVLSGESQRIKESF
                     GGLAVAMIFATLLVYMIMAAEFESFGNPFIIMITVPFSVVGVALSLFFTRTPISAPVI
                     LGMILLVGSVVNYGIILIDFMNQKRREGSTLWDAVVEGCTTRLRPVLMSSLTTILAVF
                     PLALGLSEGSELASPMAVVTFGGLGISVLLSLFVLPLIYYFIEDWRQRRADAEAAALS
                     AAEPPPAQS"
     gene            3695..>4447
                     /locus_tag="PK362_02545"
     CDS             3695..>4447
                     /locus_tag="PK362_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC73753.1"
                     /translation="MSLPRFAVRRPVATLMFFVAVCLMGLFAWIQLPVDLMPNATAGT
                     LTIYIGVRGGLPPEDIETLVTKITEDAVSSVQHLRNLLSVSRKDRSTVTLTYEPGTDL
                     SFAALEVQERLAKIKNKLPKDIEKPIVAHYSENDYPVIILALTSDKYSPERLRELVDN
                     NIKPQLMRVSGVANVEVGGGRERKILVEFDQTRLEAYRLSIRQVIDTIGKNNVNLLTG
                     KREDERSSFFIRTMGQYQTMDDLRKLVILRSPQ"
BASE COUNT          940 a         1360 c         1223 g          924 t
ORIGIN      
        1 accgggagcg aatacctcca ggccaaaatt gaacgccaaa aagcgttcaa cgaccgaaat
       61 caagcccggc gcaacgtgga aatcgacgtc cgcgaagcct actacaacat ccaaaagggc
      121 aaaatccaga tcaaaggcgc cgaagccgaa ttggattatc gggagaagga attggacatc
      181 gcccgtcaaa aagaacggat gaacctgatt gaaccggccc agtccctcgc cgccgaaagt
      241 tcctacggcg acgccgtggc caatcacgag gaagcgttgt ccttttacca ggtgtccttg
      301 gccggtctgg agcgggccgt gggcatgccg ttggagtcca tccccgaatt tcgttagccc
      361 gcctctgtcc cgcgggagag gagatcgcgc atgacgatgg ccggtttcgt cgcccgtcgt
      421 cccgtcaccg ggacgatgtt ttacatgggc gtcgcgctca tgggcgttat ttcctggttt
      481 ttcaccgcgc gggaactttt tccgtccatt tccttccctc aactcctggt catcacgcgc
      541 tacgggaacg ccgcccccga ggaaatcgag aacctcatca ccaaggtcat cgaagaatcc
      601 gtggggacgg tccccaacct caagcgggtg aggtccctgt cgaaggaagg cgtttccttg
      661 gtcacgctgg aattcgactg ggggacggac atgggcttct ctcacttgac ggcccgggaa
      721 aaaatcgacc agatcaaaga ccgcctgccc agcgaagccg aagagcccat catcaaccgc
      781 atcaacccct tcgcccaacc catgatgatt tattcggtca gcggttccct gcccattcct
      841 cgattgaccg agatcgccaa acaagtcatc aaacagcgcc tggagaaggt gacgggcgtg
      901 gcgtccgcct cgatttccgg cggtcaagag cgggaaatct tggtggaagt cgaccgcccc
      961 cggatggaag cccaagggat ttccctggcc tccatcgtgg actcgttaaa ggacgccaat
     1021 ttgaattatc ccgcggggac cgtccaggga aagttttacg agtatctcgt tcgaacgatg
     1081 ggcgaattca agaacatcgc cgaaatcgga cgaacggtca tcaaggtcga gcggccccag
     1141 gacgaggagg aacgggaccg ttacggaaac aatcgggacc gtgagaacca tccccgggag
     1201 gcccgcctca gttacctaaa cgatttggct tcggtccaag acacctttaa ggaacgcacg
     1261 agtttttccc gttacaacgg ccatgagaac atctcgatca ccattcaaaa acaagccgac
     1321 gccaacgcct tgacgacggc ccaacgcgtt cgcgtcgcca ccgaggaact tcaaacaatt
     1381 ttgaagccga agggcgtcaa cgtggagctc gtttacgacg aatcccggtt catcgaggac
     1441 gccatcagcg gggtcgccac ggacgggatc gtgggcggca tcttggcctt cttcgtgctt
     1501 tattttttcc taaaaagctg gaccgtcgcc gccatcgttg cgttggccat cccggcctcc
     1561 acgttgatta cctttaccgg gatgtttttc aaaaaaatct ccatcaacat gctctccctg
     1621 gccggactgg gccttggcat cgggaacatg gtggacaact ccatcgtcgt cgccgaaaac
     1681 atcgcccgtc atcagacgga actgggcaaa gggcgcatcg aggccgccgt cgatggcacc
     1741 gacgaggtgg ccccctccat ggtcacctcg tctttgacca acgtggcggt cttgttgccg
     1801 ctcctgttcg ccaagggcgt ggcccaattg gtcttccggg acatgttttt catcgtcacc
     1861 tgcgcgtcgt ttgcctccgt cttcatttcg atcacgttgg tccccttgct gtcggcgcat
     1921 ccggtgtcgt tcgcttttat taaaacaatg ctcgggattc gcgccgccga acccgtcccc
     1981 gggggggaaa tcccgccccc ggaagcgccg cccgccaaac cctttctcga ccgcttcccg
     2041 ctgccggttc aaaaagccct tcggtggttt ttggacgggt tttccgacga agcctacaac
     2101 cggctcatgg cgcgttacaa gtcgcttttg gagtgggtgt tgaagaaccc cagcaaaacc
     2161 gttgtgggga tcacgggggg ggccttgttg agcctcgggc ttctgggttt ccaggaccgg
     2221 gtgttcatgc ccaaagtcga ccagggccaa ttcattctta aaatccaaat gccggtcgga
     2281 acgcgcctgg aaatcaccaa cgaagtcacg gaaaaggtag aggccgtttt gaaatccctc
     2341 cccggcttcc gggaggtgac cgtcagcgtc gggtccaaca acgtcgaggc ggtcgacgct
     2401 ctgggggacc accagtccca ggccatcgtc aatttggatc gttccaaagt ggccaccgaa
     2461 gattacatcg aaaccctcaa agacacgttg gacaagcagg atctccaagg ggccgaagtc
     2521 caatacgtat tgcaggacag ccttctggcg tccgccttcg aaacggccgc ccccatcgtc
     2581 atcgaatgca aagggcccga catgaccacc ctgcgcaaga tcgccgacga tattttacgc
     2641 gacgtgaccg ccgtgccggg ggttttcggc gccaagacga gtttggccct gccgtcctcg
     2701 gaaacgcgcg tggaaatcga caagcttcgg gccgcgtcct accacctgaa caccgccgaa
     2761 attgcccgaa ccgccctgat cgggatcaaa ggctttgtgg ccaccaccta caaagaggcc
     2821 gggcaggaag tcgacgttcg tgtgcgcctt cggccggagg accgcgccga tgtggattcc
     2881 attggacggt tgacggtgtt ggccccgggg aatgtgatgg tgccgttgag cgaaattgcg
     2941 tccctggttt cggcccgagg gcccagcgaa atcaaacatt tggaccaaca acgatcgatc
     3001 gtcgtttcgg ccaacgtggc taagcgatcc accaacgacg tgatcaagga cgtcaccgct
     3061 cttctggaaa aataccacaa cctctcggac tatcaggtgg ttctttccgg cgaaagccaa
     3121 cgcattaaag agtcttttgg cgggttggcc gtggccatga ttttcgccac cctgctggtt
     3181 tacatgatta tggcggccga attcgaatcg ttcggcaacc ccttcatcat catgatcacc
     3241 gtgccgtttt ccgtcgtggg cgtggcgctg agcctcttct tcacccgaac gcccatcagc
     3301 gcgcccgtga tcttggggat gattttgctg gtgggcagcg tcgtcaacta cggcatcatc
     3361 ctgattgact tcatgaatca gaaacggcgg gagggatcca ccctctggga cgccgtcgtg
     3421 gaaggttgca cgacccgcct ccgtcccgtt ctgatgtcct ccctgaccac catccttgcg
     3481 gtctttccct tggccctggg cctttcggaa gggtcggaat tggcgtcgcc catggccgtg
     3541 gtgaccttcg gcggtttggg catttccgtt ttgctcagtt tgttcgtttt gcctttgatc
     3601 tattatttca tcgaagattg gcgtcaacga cgtgccgacg ccgaagccgc cgctctctcc
     3661 gccgcggaac ccccgccggc ccagtcctga ttccatgagt ttgcctcgct tcgcggtgcg
     3721 acggcctgtc gccacgctga tgtttttcgt cgctgtttgt ttaatgggct tgttcgcttg
     3781 gattcaactt ccggtggact tgatgcccaa cgccaccgcc gggaccttga ccatttacat
     3841 cggcgtccgg ggcggattgc ctcccgaaga catcgaaact ctcgtcacca aaatcaccga
     3901 agacgccgtt tccagcgtcc aacacctgcg aaacctcctc tcggtgtccc gaaaggaccg
     3961 ctcgaccgtc actctgacgt acgaaccggg caccgacctg agtttcgcgg ccctcgaggt
     4021 tcaggagcgc ctggccaaga tcaagaacaa actccccaag gacatcgaaa aacccattgt
     4081 cgcccactat tccgaaaacg attaccccgt catcattttg gcgttgacca gcgacaaata
     4141 cagccccgag cggctccggg aactggtcga caacaacatc aaaccgcagc tcatgcgggt
     4201 gagcggcgtg gccaacgtgg aagtgggcgg cggtcgggag cgcaaaatcc tcgtggaatt
     4261 cgatcaaacc cgtttggaag cttaccgtct ttccatccgg caggtgatcg acaccatcgg
     4321 gaagaacaac gtcaacctgt tgaccggcaa gcgggaggac gaacgaagtt ccttttttat
     4381 ccgaaccatg ggccaatacc aaaccatgga cgacctgcgg aaactggtga tccttcgcag
     4441 cccccag
//
LOCUS       DAOQLS010000089         4426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9491_length_4426_cov_3.924960, whole genome shotgun sequence.
ACCESSION   DAOQLS010000089 DAOQLS010000000
VERSION     DAOQLS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9491_length_4426_cov_3.924960"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4426
                     /locus_tag="PK362_02550"
     CDS             <1..>4426
                     /locus_tag="PK362_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73754.1"
                     /translation="MTYNKYSNMTHFDRTAIDFQGKPSQIVWDGGYDQYDRATDYDQV
                     TTDSFGHQQRLEFRKADYTIDDNMISYIEKVTEDGLTRERQFSNGTYDSKRNMIGYTE
                     KVIDSAGRVSNRVWSSVGPDFGYLKGDLRGYQETTTLENLTINTTVTGITYDALWHRN
                     GGTEVKVIKGIETDGRPADVTLTTVTTAKPDALETGLAPKDIELGIIMNKKRITDTAS
                     VQTVEGRDNATGELLRQLTVTTTRSDLTPQGDFLETRHTKGEADGDVLDTETKTHRTG
                     TVLDGFGRPVTFKEESIQNGRPSTWTERTVDKIVHNVDSNMIASHEVSLNALGVHTAL
                     SRTGMVYDQQNRLESYHMETASEAQGIHTGSTVDRTHIGYNALADVTVYHEVVAHLGS
                     SVIDTQDWHGQYNVLGLVSSSNQTIHKLGETTVNGVAQRLDVIENTSTYGMKYTRDGV
                     SLIKYTEESDSTATAGLLQVTTFDGATYDEFGRVATFNQTTRRVSQTAVSRDAKGHAV
                     VDSSAASFDSVTTTERSEATFDALGALTGYTEKSTLTDGQGRPAGGSLLHRDHLVFHP
                     MGQLLSYRGDESRLESPEVTTALTWDGRYDSLGRSAGFTETRTEQLSGGLRGLVTVTS
                     RDDVSYDDLGHVRHYTESSTRSDAPELTNRTDWSAALYDDRGNVTDSVETVQTSGRTA
                     AGGYDVTKTTRRSAVVVDDQGLTRTFRETQWDSSQPDATTTTDRQSTDFDLVGREIAS
                     VSLRHEQGQTTTPDINGVSQTMALDKWTTVVQSGRTFNDVGQLTASKELSFDGDTIAL
                     NGTETAWNALTNAQRAGLQSGALVADEMVTLTSRTGIAYNGRGLATDYTDATRELSTS
                     LDHVRSTEHRGVVYNALAQATDYTDTLRDNLTPGVTETTRVQSIEYNGFGQMIANHET
                     KTQTGDLISAVETDRHLSYDNANRLATEHTVGVNAQGVTTDRLYAVKSYNGFGRVSSE
                     SIETLQSADAGTDVLTGAPVSGFSLSTLSTKTGLYYDAKGRLVASRETKKSSDKSSVT
                     VVTDWKATDLNANNQVVASEEARVETNPAGSHRVETTRSNIRYSLPGQAQSYTDAILN
                     DADKITEVLNWSADGFNGAGLATGILEVRRRFAAGSTAPFDVTSTQKKSNLTYDAFGN
                     LIGYHQDASATDNPALKQATEWTGTFDAFGRLSSFVERSRQYDDDGALDVRTVTARDG
                     IQFDDQNRTIGYVQRSTDKLGHVSVTSWAGENDDRGLLAAFDETTTDDRGNVTHKVQE
                     DITYDAAGRNATFRQTVTDPLNTVTVTERRGTGYNDAGLMAEVVENTVATDAAGVRTE
                     TLSNQKPILFDSTGHVTQRHEEAYTSIFDENGVLVRESNKSTDMTGGRYNALGQSIQY
                     HQLAITTNPDGTFNLSVSNDFSEGLYNTLGDLTSFHQSDGQQGFSVETLTLPSNWEDL
                     ALKPEERLLWLTNLTFVVGAEKEKKGIDELNLMAPGSEG"
BASE COUNT         1022 a         1502 c         1210 g          692 t
ORIGIN      
        1 acatgacgta caacaagtat tccaacatga cgcatttcga ccggacggcc atcgatttcc
       61 agggaaaacc ttcccaaatc gtctgggacg gcggctacga ccaatacgac cgggccaccg
      121 attacgacca agtgaccacg gactctttcg gccatcagca gcgcctggaa ttccgcaaag
      181 cggactacac catcgacgac aacatgattt cctatatcga gaaagtcacg gaagacggat
      241 tgacccggga acgccaattc tccaacggga cctacgattc gaaacgcaac atgatcggat
      301 acacggagaa agtcatcgat tccgccggcc gcgtatcgaa ccgcgtctgg agttccgtgg
      361 gacccgattt cggctacctg aagggcgatc tgcgcggcta ccaggaaacc acgaccctcg
      421 aaaacctcac gatcaatacg accgtcaccg gcatcaccta cgacgcgctg tggcaccgga
      481 acggcgggac cgaagtaaaa gtcattaagg ggatcgaaac cgacggtcgg ccggcggacg
      541 tcaccttgac gacggtgacc accgccaagc ccgacgccct tgaaaccgga ttggccccca
      601 aagacatcga gttgggaatc atcatgaaca aaaagcgcat caccgacacg gcgtccgtgc
      661 aaaccgtcga agggcgcgat aacgccacgg gggaactcct ccggcaattg accgtcacca
      721 ccacccggtc ggacttgacc ccccagggcg acttcctcga aacccggcac accaaggggg
      781 aggcggacgg cgacgtgctg gacaccgaaa ccaaaaccca tcgcaccgga acggtgctgg
      841 acggtttcgg ccggcccgtg acctttaagg aagagtccat tcagaacggc cgcccctcca
      901 cgtggaccga gcggacggtg gacaaaatcg tccacaacgt ggacagcaac atgatcgcca
      961 gccacgaagt gtcgctgaac gccctgggcg tccacaccgc cctttcccgg accgggatgg
     1021 tttatgacca acagaaccgc ctggaaagct atcacatgga aaccgccagc gaagcccaag
     1081 gcattcacac ggggtccacg gtggaccgca cccacatcgg ctacaacgcc ttggcggacg
     1141 tcacggttta tcacgaagtg gtcgcccacc tgggttcgtc ggtgatcgac acccaggatt
     1201 ggcacggcca atacaacgtc ctgggcctcg tttcctcctc caatcaaacg atccacaaat
     1261 tgggggaaac cacggtcaac ggcgtggccc agcggctgga cgtcatcgaa aacacgagca
     1321 cctacgggat gaaatacacc cgggacggcg tctccctcat taagtacacc gaagaatccg
     1381 acagcacggc caccgccggc ctgctccagg tcaccacctt cgacggcgcg acttacgacg
     1441 agttcgggcg cgtggcgacc ttcaaccaaa ccacccgccg ggtctcccaa acagccgtgt
     1501 cccgggacgc caaagggcac gccgtcgttg attcctcggc cgcctccttt gatagcgtga
     1561 ccacgacgga acggtcggag gccacctttg acgccctggg cgccttaacg ggctatacgg
     1621 aaaaatccac cttaacggac ggccaaggcc gcccggccgg gggaagcctg ttgcaccggg
     1681 accacctggt tttccacccc atgggtcaac ttctttccta ccgaggggac gaatcccgcc
     1741 tggaatcccc cgaagtgacg accgctttga cctgggacgg ccgctacgat tcgctgggcc
     1801 gctccgccgg gtttaccgaa acgcgcaccg aacagctgtc cgggggcctg cggggcctgg
     1861 tgaccgtcac ctcccgggac gacgtttcct acgacgattt gggccacgtc cgccactaca
     1921 cggagtcctc cacccgatcc gacgcgcccg aactgaccaa tcgcacggat tggtccgcgg
     1981 cgctgtatga cgatcgcggc aacgtgaccg actccgttga aaccgtccaa acctcgggcc
     2041 gcaccgccgc cggcgggtac gacgtgacca aaacgacccg tcgctcggcc gtggtggtgg
     2101 acgaccaggg gttgacgcgc accttccggg aaacccaatg ggattcctcc caaccggacg
     2161 cgaccacgac caccgaccgg caatcgacgg atttcgacct ggtgggccgg gaaatcgcct
     2221 ccgtgtccct tcggcacgaa cagggccaaa ccacgacgcc ggacatcaac ggcgtttccc
     2281 agaccatggc cctggacaaa tggaccaccg tcgtccaatc gggacggacc tttaacgacg
     2341 tcggccaact cacggcgtcc aaggaattga gcttcgacgg cgacaccatc gccttaaacg
     2401 gcacggaaac cgcctggaac gctttgacca acgcccaacg ggccggcctg cagtccggcg
     2461 ccctggtggc cgacgaaatg gtcaccctga cctcccggac gggcatcgcc tacaacggac
     2521 ggggcctcgc caccgattac accgacgcca cccgggaact ttcgacgtcc ttggaccacg
     2581 tccgatccac cgaacaccgc ggggtcgtct acaacgccct ggcccaggcc accgattaca
     2641 ccgacaccct tcgcgacaat ctgaccccgg gcgtcaccga aacgacacgc gtccaatcga
     2701 tcgaatacaa cgggtttggg caaatgatcg ccaaccacga aacgaaaacc cagacgggcg
     2761 atttgatctc cgccgttgaa accgaccgcc atctttcgta cgacaacgcc aaccgactcg
     2821 ccaccgagca caccgttggc gtcaacgccc agggcgtgac gaccgatcgc ctctacgcgg
     2881 taaaatccta caacggattc ggccgggtgt cgtcggagtc catcgaaacc ttgcaatcgg
     2941 ccgacgccgg caccgacgtt ttgaccggcg cgccggtgtc cggattcagc ctttccacgc
     3001 tttcgaccaa gaccggcctt tattacgacg ccaaaggccg cctggtcgcc tcccgggaaa
     3061 ccaagaaatc cagcgacaaa tccagcgtca cggttgtcac cgattggaaa gccacggacc
     3121 tgaacgccaa caatcaggtg gtcgcttccg aggaagcccg cgtcgaaacc aaccccgcgg
     3181 gttcccaccg cgtcgaaaca acccgctcga acatccgcta cagccttccc ggtcaggctc
     3241 aatcctatac cgacgccatc ctcaacgacg cggacaaaat caccgaggtt ttgaattggt
     3301 ccgcggacgg gttcaacggc gcggggttgg ccaccggcat tttggaagtc cgccggcggt
     3361 ttgcggccgg cagcaccgcg ccctttgacg tgacctccac ccagaaaaaa tcaaacctga
     3421 cctacgacgc cttcggcaat ttgattgggt accatcagga cgcttcggcc accgacaatc
     3481 ccgccctcaa gcaggccacg gaatggaccg gcacctttga cgcctttggc cgcctctcgt
     3541 cctttgttga acggtcccgc cagtatgacg acgacggggc gttggacgtt cgcaccgtga
     3601 ccgcccggga cggcattcag tttgacgatc aaaaccgaac catcgggtat gtccaacgtt
     3661 ccaccgacaa attgggccac gtcagcgtga cctcctgggc cggggaaaac gacgaccgcg
     3721 gcttgttggc cgccttcgat gagaccacga cggacgatcg cgggaacgtg acccacaagg
     3781 tccaagagga catcacgtac gacgccgccg gccgtaacgc cacctttcgc caaaccgtca
     3841 ccgatcccct gaacaccgtg accgtgaccg aacgccgagg caccggctac aacgacgcgg
     3901 ggctcatggc cgaagtcgtt gaaaacacgg tggccaccga cgcggcgggg gtgcgtaccg
     3961 agacgctgtc caaccaaaaa ccgattctct tcgacagcac gggtcacgtg actcaacggc
     4021 acgaagaggc ctacacctcc attttcgatg aaaacggcgt cctggtccgg gaatcgaaca
     4081 aatcgacgga catgacggga gggcgctaca acgccctggg ccagtcgatt caataccatc
     4141 aattggccat caccaccaac ccggacggaa ccttcaatct cagcgtgtcc aacgactttt
     4201 cggaagggct ctacaacacg ttgggcgatt tgacgtcttt ccaccaatcc gacggtcaac
     4261 aggggttcag cgtggaaacc ctgacgctcc cttccaattg ggaggatctg gctctcaaac
     4321 ccgaagagcg cctcctctgg ctgacgaacc tcaccttcgt ggtcggggcc gaaaaggaaa
     4381 agaaagggat cgacgaattg aatttgatgg cccccggttc cgaggg
//
LOCUS       DAOQLS010000090         4403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9582_length_4403_cov_3.727921, whole genome shotgun sequence.
ACCESSION   DAOQLS010000090 DAOQLS010000000
VERSION     DAOQLS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_4403_cov_3.727921"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PK362_02555"
     CDS             <1..663
                     /locus_tag="PK362_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC73755.1"
                     /translation="MVVDDDKDIRRLVENILSKEGFVTVGAENAADALKKIQNSKPDL
                     IILDLQLPDKDGFEVCKQLRADPATRYVPVVFLTVQNVDSYKIAGLEIGADDYITKPF
                     NQTELVARVKAVLRRVDWRDKKEAALRDGQLAVDMEKHSVHMDAKPLDLSPKEFDLLV
                     SLLRNHGKVMTRAELSETVWGHEYFGNTRTVDVHVGRLRKKLGKMGEKIRTVERIGYR
                     YE"
     gene            complement(695..1066)
                     /locus_tag="PK362_02560"
     CDS             complement(695..1066)
                     /locus_tag="PK362_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC73756.1"
                     /translation="MAKKRVLIIDDDPAMSGLLEEFCVEMGCDVLCVNDSRHAFEAVR
                     RFQPDLITLDLEMPHKDGLELLREFRADPQAKTIPVLIVSIMAQEAPIAAEAVLGKLT
                     KPVPFGTFLDKVRKLLIPAIS"
     gene            1236..>4403
                     /locus_tag="PK362_02565"
     CDS             1236..>4403
                     /locus_tag="PK362_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73757.1"
                     /translation="MASSLKNKLIKQQFAALTAGCFLLTNLGTPYAQATTSSSFWESR
                     KKTSDIKKELPDFSKPLELPDSDDGDLGFRLEDGLVKERFWPGQENPGPWRVGGRTLP
                     FIVHVQDAHRNVDAQRTLGRIVRSLAEKNGGSALVCVEGAWGELDPGLLKVFPDRELR
                     DRAAEEFLRVGWLTGEEHLAVTADDLALRLVGVDDPLLHRANRAARDRAEPLAGLAHR
                     RLARLADRAVRRQAALWPQSLRDLEAIRRRYEADEDSLAHYVRALVSRLPPGERSGYP
                     ALDAFGRLSLLEDRYEPDRARQDLRDLAARSSALAPADRAALRAGLQAGPRERSRAIS
                     DLIRRIGGTGSAQWPALRAHAEALALRAAIDAGRLASEVESAHRRVVDDLLRQRPESQ
                     RPDLLGLLAATDWIHSRQRLWSFEMTPREWRAARRDWARYGWADVEALLGDAAPAESG
                     DAVRAALRDAEEHLRDFYETALQRNRAMADNTLAAARRWRADRVVLITGGFHTPGLTD
                     LLRQAGANYAVVCPVFNPRPAPPVHTATLRDQSRLEDLADRQTLAAAQWAIASGEKPV
                     ATLGEWRRSWSRALTNPTDRRWIAGLNLISRESGPDGSWTAAVGSASANDARRIQFSG
                     TAGLRSIPLSPPPIPSISDVSAAGRQTVLKMQGGVRRAFATVKRFGRPVLQRSWPWVA
                     AGAVLLGAAWIFLPALFPGNGAEVVFSMGSAQWAAMTGVIFPPWGRHLGVWRLDEHSR
                     GPRDQGNNDWFDELQRLLIALEESDRRLAEAIAAKQKAEISVAQLRLSEARLNLQQFM
                     RRRLAASDEDTATLYLRLEGPKRDLLVRAVGAQVLGRLIHGMTGHFFERARRGLRRRR
                     ADLERLLQLEESLPRASAGERERILGDLEKTLVALDRNAQGLIEGLQTFIGTPNVPDI
                     PAPDLAKIKTEARAKLQSLADHRRLDAQWADTLRSALTTGGKTDPLPPLAAHRARHRA
                     ALTETEMDALSLSNPYIFRDPIYAHRRSRALLHGLRNREIRLIQRALTHDRATLDAVG
                     PIYHAADLLTEPLGDAVQDLTGMAQH"
BASE COUNT          811 a         1388 c         1350 g          854 t
ORIGIN      
        1 atggtggtgg acgacgacaa ggacattcgc cggttggtgg aaaatatttt gtccaaagag
       61 gggttcgtga cggtgggcgc cgaaaacgcc gccgacgccc tcaagaaaat tcaaaattcc
      121 aagccggatt tgatcatcct ggatttgcaa ttgcccgaca aggacggttt cgaagtgtgc
      181 aagcagctgc gggccgatcc ggcgacgcgt tacgtcccgg ttgttttcct gaccgtccaa
      241 aacgtcgatt cctataagat cgccggactg gagatcgggg ccgacgatta cattaccaaa
      301 ccgttcaacc agaccgaact ggtcgctcgg gtcaaggcgg tccttcgccg ggtggactgg
      361 cgcgataaaa aagaagccgc gctgcgcgat ggccagttgg cggtggacat ggaaaaacat
      421 tccgtccaca tggacgccaa acccttggac ctctccccca aggaattcga tttgctggtg
      481 tccctccttc gtaaccacgg caaggtcatg acgcgggcgg aattgtcgga aaccgtgtgg
      541 ggccacgagt atttcggcaa cacccgcacc gtggacgtcc acgtgggccg tttgcgcaag
      601 aaattgggga agatgggcga gaaaatccgt acggtggaac gaatcggcta tcggtacgag
      661 taggtttccc cccccgtcgc cgggccgaag ggcgtcagga gatggcgggg atcagcagct
      721 tgcgaacctt gtcgagaaag gttccgaaag gaaccggttt cgtcagtttc cccagcaccg
      781 cttcggccgc gatgggcgct tcctgggcca tgatggacac gatgagcacc ggaatggttt
      841 tggcctgggg gtccgcgcga aattcccgca acaattccag gccgtccttg tggggcattt
      901 ccaagtccaa ggtgatcaag tcgggttgaa atcgacgcac cgcttcgaag gcgtggcggc
      961 tgtcgttcac gcataaaacg tcgcaaccca tttccacgca aaactcctcc agcaaaccgg
     1021 acatggccgg atcgtcgtca ataatgagca cgcgtttctt tgccatttcc ccctcctcgg
     1081 cgcttcccgt ttccctataa atttagatca aaaagtttcg gcccgcctta attaattgta
     1141 acggaattga aacaaattat cgaaatgtta tttacattac atttacattt tattcaggcc
     1201 ctctttatct cgcttttgta tgtttaaagc gaaatatggc atcttccctc aaaaataaat
     1261 taattaaaca gcaattcgcc gcattgacgg cgggctgttt tttgctgaca aaccttggaa
     1321 cgccttacgc ccaggcgacc acgtcgtcgt ccttttggga atccagaaaa aaaacctccg
     1381 atatcaaaaa agaactcccc gatttttcaa agcccctgga attgccggat tcggacgatg
     1441 gagatcttgg cttccgtctt gaggacggat tggtgaaaga gcgattttgg cccgggcagg
     1501 aaaatcccgg gccctggcgc gtgggcggcc gaaccctgcc cttcatcgtc cacgtgcagg
     1561 acgcccaccg caacgtcgac gcccaacgaa cgctcggccg gatcgtgcgg tccctggccg
     1621 aaaaaaacgg cggttccgcc ttggtttgcg tggaaggcgc ctggggagag ctcgatcccg
     1681 gattgctgaa ggtcttcccc gaccgggagc ttcgcgatcg agccgccgag gaatttttac
     1741 gcgtcggctg gttgacgggc gaagaacact tggccgtgac cgccgacgac ctcgctctgc
     1801 ggctggtggg cgtcgacgat ccattgttgc accgggccaa tcgcgcggcc cgtgaccggg
     1861 cggagcccct ggccggcctc gcccaccggc ggttggcccg cctggccgac cgggcggttc
     1921 gtcgtcaggc ggccctttgg ccccagtcgc ttcgggacct cgaggcgatt cggcgacgct
     1981 acgaggccga tgaggattcc ctggcccatt acgttcgcgc tttggtgtcg cgtcttcccc
     2041 ccggcgaaag gtccggttac ccggctctgg acgcctttgg gcgactcagt cttcttgaag
     2101 accgatacga accggaccgg gcccgtcagg atttgcggga tctggccgcc cgatcgtccg
     2161 ctttggcccc cgccgaccgg gccgctctgc gggccggtct tcaggcgggg ccacgggagc
     2221 gctcccgcgc catctcggat ttgattcgac ggatcggcgg tacgggatcg gcccaatggc
     2281 cggccctgcg ggcccacgcg gaagccctcg ccctacgcgc cgcgatcgac gccggtcgtc
     2341 tggcgtcgga agtggagtcc gcccaccgcc gcgtcgtgga cgacctcctc cgtcaacgcc
     2401 cggaatccca gcgtccggac cttcttggtc ttctggcggc aaccgattgg attcactccc
     2461 gacaacgtct ctggtccttt gaaatgacgc cccgggaatg gcgggccgcg cgtcgggact
     2521 gggcgcgata cggctgggcc gacgtggagg ccctcctggg cgacgccgcc ccggcggaat
     2581 ccggggacgc cgtgcgggcg gcactgcggg acgccgaaga acatttaaga gatttttacg
     2641 agacggccct tcaacgcaac cgggccatgg cggacaacac cctggccgcg gcgcggcgtt
     2701 ggcgcgcgga ccgcgtcgta ctcatcacgg ggggctttca cacgccgggg ttgacggacc
     2761 tcctccgcca ggccggcgcc aattacgccg tggtgtgccc ggtcttcaac ccccgtcccg
     2821 ctccccccgt ccacaccgcc acccttcggg accaaagccg cctggaggac cttgccgacc
     2881 ggcaaaccct cgccgcggcc cagtgggcga ttgcgtccgg cgaaaaaccc gtcgcgactc
     2941 tgggcgaatg gcggcgatcg tggtcccggg cgttgacgaa tcccacggac cgtcgatgga
     3001 tcgccggatt gaatttgatt tcgagggaat cgggccccga cggttcgtgg accgcggcgg
     3061 tggggtcggc atcggccaac gacgcccggc ggattcaatt ttcggggacc gcgggtttgc
     3121 ggtccatccc cctttcccct cctccgatcc cgtcgatttc ggacgtctcc gcggcgggcc
     3181 ggcaaacggt cctaaaaatg cagggaggtg ttcgtcgtgc cttcgcgacg gtcaaacggt
     3241 ttggtcggcc cgtcctccag cgctcctggc cgtgggtggc cgcgggcgcg gttcttttgg
     3301 gcgcggcgtg gatttttctc ccggccctgt tccccggcaa cggggcagag gttgttttct
     3361 caatggggtc cgcccagtgg gcggcaatga cgggggttat tttcccgccc tggggccgcc
     3421 atttgggcgt ttggcggttg gacgagcatt cccggggccc gcgggatcaa gggaacaacg
     3481 attggtttga cgagttgcag cgtttattaa ttgctctgga ggaaagcgat cgacgattgg
     3541 cggaggccat tgccgccaaa cagaaagccg agatttcggt cgctcaactc cggttgtccg
     3601 aggcccgcct taaccttcaa caatttatgc ggcgtcgatt ggccgcgagc gacgaggaca
     3661 cggcaactct ttacctgcgt ctggaggggc ccaagcggga tttgctggtg cgagcggtgg
     3721 gggcccaggt tctgggacgt ctcattcacg gtatgaccgg gcatttcttc gaacgcgcgc
     3781 gacggggact ccgccgtcgc cgcgccgatt tggaacggct cctccaactg gaagaatcgc
     3841 tcccgcgcgc ctcggccggg gaaagggaac ggattctggg cgatctcgaa aaaaccctcg
     3901 tggcgctcga ccgcaacgcg cagggattga tcgaaggcct gcaaacgttc atcggcacgc
     3961 ccaacgtccc cgacatcccc gccccggatc tggccaaaat caaaacggag gcccgggcca
     4021 aattgcaatc gttggccgac catcgccgtc tcgatgccca atgggccgat acccttcgaa
     4081 gcgcgctgac gaccggaggg aaaacggacc cccttccgcc cctcgcggcc catcgggccc
     4141 gtcatcgggc ggccttgacc gaaacggaaa tggacgctct ttcgttgtcc aacccctata
     4201 ttttccggga cccgatttac gcccaccgtc gatcccgggc cctccttcac ggcctccgca
     4261 accgggaaat acgattgatc caacgggccc tcacccacga tcgcgccacc ttggacgccg
     4321 tcggccccat ctatcacgcc gcggaccttc tgacggagcc cctcggcgac gcggtgcagg
     4381 atttgaccgg catggcgcaa cac
//
LOCUS       DAOQLS010000091         4400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9591_length_4400_cov_4.347986, whole genome shotgun sequence.
ACCESSION   DAOQLS010000091 DAOQLS010000000
VERSION     DAOQLS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9591_length_4400_cov_4.347986"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK362_02570"
     CDS             <1..469
                     /locus_tag="PK362_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC73758.1"
                     /translation="LSWRLVRAEGQRRFVCGGCRSISYQNPKIVAAALPVWKNKIVLL
                     RRAIEPAKGLWSHPAGFMELGETVQKAAIRETREEIGCRVRLAGPARLYSYDDAAVVT
                     VVYDAWVVGAGPRAGAESLEVKAFAADEIPWDRLAFRSTYHVLRDWADRRSKS"
     gene            complement(466..975)
                     /locus_tag="PK362_02575"
     CDS             complement(466..975)
                     /locus_tag="PK362_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73759.1"
                     /translation="MVPWALPVLASNLPLYERVLWVDTSGRFDPRPLLQQADDWGIDR
                     FFYINRIQVAQPRTTDHLERFIVSELLPRSAGDQPVVIADPFHLWDVDGFSPAEKEDR
                     FLRFEDLMANILPRCLVLSEERRSNMSGFNQRLYLQAARRARLEMKDGFWSVVELSEK
                     LSSPVKNPV"
     gene            complement(1108..1701)
                     /gene="lexA"
                     /locus_tag="PK362_02580"
     CDS             complement(1108..1701)
                     /gene="lexA"
                     /locus_tag="PK362_02580"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNC73760.1"
                     /translation="MDNLTSRQQEIYNFIASEIQTAGLPPTVREIAGHFDIFPKAVQD
                     HLAALEKKGVLRRAKDRARGLIMEARGAVQKLRLPILGRVPAGQPLEAIADVEDYLAV
                     DEAIAKSANFALRVKGDSMFPEINDGDVVLVQSTNVAQNGDVVVATVDDNEATVKRLR
                     RVGGDVYLQPANPAYPIIRGKDITVVGRVTSLIRTFF"
     gene            1700..2881
                     /locus_tag="PK362_02585"
     CDS             1700..2881
                     /locus_tag="PK362_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73761.1"
                     /translation="MSATIQKNSVRVNFRLAMGLMALAAGPLRADITGPAIVDNDYRL
                     AFNPSVAVGSSRKVAMGGAYTGIAEGADSLWDNPVAAGFRRPGDESDWDYDLMAGSTI
                     FNGDDVDNNGHLTHAFRRNSVVDVGAQVRKSRVGVGFYVVNQQYDVGGPAGVERLSFN
                     VGTIAVGWASPMENFFWGVGLRLAALESKPKGSRIKTLHLSGSGGSVGTLWNPGRGPW
                     RLGMSFAGAIHDNNEFPANATAPVVNNGLIIPRQVTLPVVGSLGVSYHFARGTFWSDR
                     PLLMGLQVNAVAPTRGAVGVESFIEQRVQYTGKKRTYSYHLGGEEEIWPRRVRLRLGT
                     YYEPSRFEGVKGRVHATGGVEIRLFHFNFLGSHDVAFSYAADNAPRYFTQIFNVGFWQ
                     F"
     gene            3080..>4400
                     /locus_tag="PK362_02590"
     CDS             3080..>4400
                     /locus_tag="PK362_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73762.1"
                     /translation="MLTLDRCLEKALAQNPALTLAEDDFAIARARARAAAVSLFPAVT
                     AKADETTGRAEQGVDNPPFVQRSYGVQATQTIFSGGKLWGTQRQASLGAEIARWQLEK
                     QRLDVRHAVTEAYWRVAGLQQALRAHRDAHGLLQEDLEKAVRHDLSEARSARIELLST
                     RAQNRESEAALAEVQESLLEARQALWDALGERTATEYQVPEEMPAGFVDVDESEALRL
                     ARDHRPDTRIADRMMESARFARSVARSGVFPKIDLNGFYGRSASAFIETDPLIYKKDW
                     NVGLSANWAFIGNSVKYSAYREKTSPRLGESSRTETDNHALSLSLGDALGVGVVHREG
                     RRSFHEEEWRYEKSRRDADNDVRLAVRRTATARSRYEAARAKLEEALQEFKDTRSLLQ
                     EDRAHLGDLAAARNRVAFAQAAQAQSQAQYLAAVSALNRAVGLSDKYR"
BASE COUNT          833 a         1258 c         1427 g          882 t
ORIGIN      
        1 attgtcctgg cgcctggtcc gggcggaggg ccagcggcgg tttgtgtgcg gcgggtgtcg
       61 atcgatttcc tatcagaacc cgaaaatcgt ggcggcggcc cttccggttt ggaaaaacaa
      121 gattgtttta ttgcggcggg ccatcgaacc ggccaaaggc ctctggagcc acccggcggg
      181 ttttatggaa ttgggggaaa cggttcagaa agcggccatt cgggaaaccc gggaggaaat
      241 tggctgccgg gttcgcctgg ccgggccggc gcgcctctac tcctacgacg acgcggccgt
      301 ggtgacggtg gtttacgacg cttgggtggt gggggccggt ccccgggcgg gagcggagtc
      361 cttggaagtc aaagccttcg cggcggacga aattccctgg gaccgattgg cttttcgaag
      421 cacttatcac gttctccggg attgggcgga ccggcggtcg aaaagttaga ccgggttttt
      481 taccggcgac gacagtttct cggaaagctc caccacggac caaaatccgt ccttcatttc
      541 caatcgggcg cgccgggcgg cttgtagata aagtcgctgg ttgaacccgg acatgttcga
      601 gcggcgttcc tcgctcagca ccagacaccg cggcaggatg ttggccatca ggtcctcaaa
      661 ccgaagaaaa cggtcttcct tttcggcggg gctgaacccg tccacgtccc aaaggtggaa
      721 cggatcggcg atgacgacgg gctggtcgcc ggcgcttcgg ggcaacagtt ccgacacgat
      781 aaagcgttcc aggtgatcgg tggttcgggg ttgggcgacc tgaattcgat tgatatagaa
      841 gaaccgatcg atgccccagt catcggcctg ctgaagcaga ggccgcgggt caaaccgccc
      901 cgacgtgtcc acccagagga cccgttcgta gagaggcagg ttggaagcca ggacgggaag
      961 ggcccaggga accattccgc tggggccgga agccacggaa tagccggcgc gtgtttgggg
     1021 gtgttgcggc ggagccagga ggctctgcag ggcaagtctc ggatcgttta tcatcgtatc
     1081 cctcggcgtg agagttgtac agatggttta gaaaaaagtt cttatcaggc tggtgacgcg
     1141 gccaacgacg gtgatgtcct ttcctcgaat gatggggtag gccggattgg ccggttgcag
     1201 gtaaacgtcc ccgccgacgc gacgcaggcg tttaacggtg gcttcgttgt cgtccacggt
     1261 ggccacgacc acgtcgccgt tttgggcgac gttggtgctt tgaaccagga ccacgtcgcc
     1321 gtcgttgatc tcgggaaaca tgctgtcccc tttgacgcgc agggcaaaat tggcgctttt
     1381 ggcgatggct tcgtccacgg ccaaataatc ttccacgtcc gcgatggctt ccaggggctg
     1441 gcccgccggc acccggccga gaatcgggag gcgaagtttt tggacggctc cgcgggcttc
     1501 catgatgagc ccccgagccc ggtccttggc ccggcgcaat accccttttt tctccagggc
     1561 ggccagatgg tcctgaacgg ctttgggaaa tatatcgaaa tgaccggcga tttcgcgcac
     1621 cgtggggggg agtccggcgg tctggatttc cgaggcgatg aaattgtaga tttcttgttg
     1681 tcgggaggtg agattgtcca tgagtgcgac tatacaaaag aatagtgttc gcgtcaactt
     1741 ccgcctggcc atgggtttaa tggcgttggc ggcgggcccc ttgcgggcgg atattaccgg
     1801 tccggcgatc gttgataacg attaccggtt ggcgttcaat ccgagcgtgg cggtgggttc
     1861 cagccgcaaa gtggccatgg gcggcgccta caccggcatt gccgaaggtg cggacagtct
     1921 ttgggacaat cccgtggccg ctggatttcg gcgccccggg gacgaatcgg attgggatta
     1981 cgatttgatg gccggcagca cgatttttaa cggcgacgac gtggacaaca acggccattt
     2041 gacccacgct ttccgaagga attcggtggt cgacgtcggc gcccaggtgc ggaaaagccg
     2101 cgtcggggtc ggtttttacg tcgtcaacca acagtacgat gtcggcgggc cggcgggcgt
     2161 cgaacgcttg tcgttcaacg tcggaacgat cgccgtcggt tgggcttccc ccatggagaa
     2221 ttttttctgg ggggttggat taaggttggc ggctttggaa tccaaaccca aaggatcccg
     2281 aatcaaaacg ctacatctgt cgggcagcgg ggggagcgtg gggaccctct ggaaccccgg
     2341 gcggggcccc tggcgcctgg gaatgtcttt cgctggggcg atccacgaca acaacgaatt
     2401 tccggcgaac gcgaccgccc cggtcgtcaa caacggtctc atcatccccc gacaggtcac
     2461 ccttcccgtc gtggggtctc tgggtgtttc ctaccatttt gcccgcggca cattttggtc
     2521 cgatcggcct cttttaatgg gccttcaagt caacgccgtg gcccccaccc ggggcgcggt
     2581 gggtgttgaa tcctttatag aacaacgggt gcaatacacg ggaaaaaaac ggacctatag
     2641 ttatcatttg ggcggggagg aagaaatttg gccccgtcgc gtgcgccttc gattgggcac
     2701 ctattatgag cccagccggt ttgaaggggt gaaggggcgg gttcacgcca ccgggggcgt
     2761 cgaaatccgc ctgttccatt ttaatttcct gggaagccac gacgttgcct ttagctacgc
     2821 cgccgacaac gccccccgct attttacgca gatattcaac gtcggttttt ggcagtttta
     2881 gttcttatcc gccgaaggcc gacgggccgt ggtataataa aagagtatca acgattccca
     2941 ggggacttcc gactacttcc caatgaaaaa caataatcat catcgaatcc gaacgatact
     3001 ttccctttgg acgcggggag cgttcctttt gggactgatt ttggttttcg ctgggtttgc
     3061 gaaggcggag gacgccgccg tgctcacgct ggatcgttgt ttagaaaaag ctctggccca
     3121 aaaccccgcc ctcactctgg ccgaggacga tttcgccatc gcgcgggccc gggcccgggc
     3181 cgccgcggtt tccttgttcc cggcggtgac cgccaaggca gacgaaacca ccgggcgcgc
     3241 cgaacagggc gtcgacaatc ccccgttcgt tcaacgctcc tacggcgtcc aggcgacgca
     3301 gacgattttt tcggggggga agctttgggg aactcaacgc caggcttcct tgggcgcgga
     3361 aatcgcccga tggcaattgg agaaacagcg gctcgatgtt cgacacgccg tgaccgaggc
     3421 ctattggcgg gtggcggggc ttcaacaagc cctgcgcgcc caccgggacg cccacgggct
     3481 gctgcaggaa gatctggaaa aagccgtgcg acacgatttg agcgaagccc gttcggcccg
     3541 gattgaactg ttgtcgaccc gagcgcaaaa ccgcgaaagc gaggcggccc tggccgaggt
     3601 tcaggaaagc ctcttggagg cccggcaggc cctgtgggac gccttgggcg agcgcaccgc
     3661 gacggaatac caagttcccg aagaaatgcc ggcggggttc gtggacgtgg acgaaagcga
     3721 ggccctccgc ctggcccggg accaccggcc cgacacgcgc attgccgatc gaatgatgga
     3781 gtcggcccgt ttcgctcgtt ccgtggcgcg cagcggtgtc tttccaaaaa tcgatcttaa
     3841 cggtttctac gggcggtccg cctccgcctt tatcgagacg gatccattga tctacaagaa
     3901 agattggaac gttggcctct ccgccaattg ggcctttatc ggcaattccg tcaagtattc
     3961 cgcctatcgg gagaaaacca gcccgcgcct tggcgagtcc agccggacgg aaaccgacaa
     4021 ccacgccttg agcctttccc tgggcgacgc gctgggcgtc ggagtggtcc atcgggaggg
     4081 acggcggtct tttcacgagg aagagtggcg atacgaaaaa tcccgccggg acgccgacaa
     4141 cgacgttcgg ctggcggtgc gccgcaccgc cacagcgcgt tcccgctacg aagcggcccg
     4201 ggccaaattg gaggaggcgc ttcaagaatt taaggacacg cgttccctcc tccaggagga
     4261 tcgggcgcat ttgggggacc tcgcggccgc ccggaaccgt gtggctttcg cccaggccgc
     4321 ccaggcccaa tcccaggccc agtatttggc ggccgtctcg gcgctgaacc gcgcggtggg
     4381 cctatcggac aaataccggg
//
LOCUS       DAOQLS010000092         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9787_length_4344_cov_4.132432, whole genome shotgun sequence.
ACCESSION   DAOQLS010000092 DAOQLS010000000
VERSION     DAOQLS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9787_length_4344_cov_4.132432"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PK362_02595"
     CDS             complement(<1..781)
                     /locus_tag="PK362_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase subunit L"
                     /protein_id="HNC73763.1"
                     /translation="MPKRTDINTILILGSGPIVIGQACEFDYSGAQAVKALKKEGYRV
                     VLINSNPATIMTDPEFAHATYVEPLIPSMVERIIEKEKPEVILPTLGGQTALNLAVEL
                     HERGSLKKYGVELIGAKIDAIKRAEDRELFKKTMMGIGLEVPQSGVARSLDEAQKIAE
                     SIGFPLIIRASFTLGGVGSAIVYTREEFVAAARRGLDASPIHEILLEQSVIGWKEYEL
                     EVMRDKADQCVIICSIENMDPMGVHTGDSITVAPAQTLSDPE"
     gene            complement(845..1816)
                     /locus_tag="PK362_02600"
     CDS             complement(845..1816)
                     /locus_tag="PK362_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73764.1"
                     /translation="MRRAALSTLFLAFGFTGLWGASDPRTAGNSLLETADARVTGLSG
                     AATALTGDLGVAAVNPAALPTLVGAEASTQFESAPGDVRTGMASFGRGNGRAGWAATG
                     LFFDAGKIDVVPLSGPGYTRRAQQDWVGALTGGIALGESLRLGVAGKALSSTLVRDYH
                     AAVFAGDAGFQWDVSRNLTLGGAVTNAGPKLTYRNQGDPLPTEYRAGAAVKFALGSGG
                     DESEDPDSPWYTKIRSDKNYITVTGDAVADRFGAVAGAVGLEWDYARRAILRGGMRLG
                     QTGDSFSAGLGFWMKGGRFDYSFQAVGDLPDRHRVTVSWFWKKKSEE"
     gene            complement(1818..3431)
                     /locus_tag="PK362_02605"
     CDS             complement(1818..3431)
                     /locus_tag="PK362_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73765.1"
                     /translation="MNKAALLLGLALAAAPAAAAVSTDTVSTSVTVSSSVFMQTGVAI
                     DTRNNVHLVFVDTATLTLKYAQLANGTTTWALSSVSAAQVGPQCALALENGLLPHVVY
                     YQTSGTPGVMHGRLSGGSWSTDTVEAFTGTNTFVSIAVGADRNPRVAYYNSVSSATVF
                     GARSAGVWSTFTVLTSTFGGPVDIALTSTDSARIALVNGSTVTSYRELSYARQTGAST
                     FAITTPYVIGTDTTTLGGLALALDSLGRAHLAVYDTFSDGLDYIEFDTAGSTVAAVVV
                     DSGSGTGFFCDIAVDSADDPRIVYYSTAGLKTATAAGSWAPNVLDAGAVVGVGASVVF
                     NRFDHYLAGYLNADLSEAKFVTDAIRGLSLSGTVLDFSFAPIPGVTLSLAGNIASTGV
                     SISTATGAYSADHLFEGTYSLTPSLTGYSFQPNARSYSVLQSSQSLQNFKGGPVAVSA
                     VGNLFDPSAGESATLNFSVLPGHVFAGVYTLQGRPVKTLLDEDKSAGNYSVVWDGRAS
                     DGDIVASGIYLVRFETDNLKQILKMAVVK"
     gene            complement(3428..>4344)
                     /gene="carA"
                     /locus_tag="PK362_02610"
     CDS             complement(3428..>4344)
                     /gene="carA"
                     /locus_tag="PK362_02610"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNC73766.1"
                     /translation="SKKPHVAGFVAREFSPRASHWGSTADLEGYMKTHGVIGIDGLDT
                     RALVRHLREVGACRGIVSTSTEKPKALVERAKKSAAMVGADLVREVSCAKPYAWTPGK
                     PGNGAARRVALMDFGVKYNIMNSLAALGCDVTVFPATTSLADLLAFKPDGVMLSNGPG
                     DPAAVTYAIDTLKGLIRHNEGAAAPIPVFGICLGHQLLGLALGGKTYKLKFGHRGANH
                     PVKDLTTGKVEVTTQNHGFVVDIDSLAKGDVELTHINLNDNTLEGLRHKHLPLFSVQY
                     HPEAAPGPHDAHYLFDRFLGLMKEPARA"
BASE COUNT          786 a         1380 c         1396 g          782 t
ORIGIN      
        1 attccgggtc cgacaaggtc tgcgccgggg cgacggtgat ggaatccccg gtgtggacgc
       61 ccatggggtc catgttttcg atggagcaga tgatcacgca ctgatcggcc ttgtcccgca
      121 tgacctccaa ctcgtactct ttccacccga tcacgctttg ttccaacagg atttcgtgga
      181 tggggctcgc gtccagcccc cgccgggcgg cggcgacgaa ttcctcccgg gtgtagacga
      241 tggcggaccc cacgcccccc agggtgaagg acgcgcggat gatgagcgga aatccgatgc
      301 tttcggcgat tttctgggct tcgtccaggg aacgggccac gccgctttgg ggcacttcca
      361 ggccgatgcc catcatggtt ttcttgaaga gctcccggtc ttcggcccgc ttgatggcgt
      421 cgatcttggc gccgatcaat tccacgccgt attttttgag actgccccgt tcgtggagct
      481 ccacggcgag gttcaacgcc gtctggccgc ccagggtggg caaaatcact tcgggttttt
      541 ccttttcaat gatgcgttcc accatggagg gaatcagcgg ctccacgtag gtcgcgtggg
      601 cgaactccgg atccgtcatg atggtggcgg ggttggaatt gatgagaacc acccggtagc
      661 cttccttctt cagagccttg acggcctggg cgccggaata gtcgaactcg caggcttggc
      721 cgatgacgat ggggccggag ccgagaatga ggatggtatt gatgtccgtt cgtttgggca
      781 tgggaaacgg gccttcgctt tcaagagtcc gggccccggg ggaacacccc gggccggttc
      841 aaatttattc ttccgacttc tttttccaaa accaggacac ggtcacccgg tggcggtccg
      901 gaagatcccc caccgcttga aaggagtaat cgaaccgccc ccccttcatc caaaaaccga
      961 gccccgccga aaaactgtcg cccgtttgcc ccaggcgcat cccgccgcgc aaaatggccc
     1021 ggcgggcgta atcccattcc aacccgacgg ctcccgccac cgcgccgaag cggtcggcga
     1081 cggcgtcccc ggtgaccgta atatagtttt tgtccgatcg aatcttcgtg taccagggcg
     1141 agtccggatc ctccgactca tcgcccccgc tgcccagggc gaacttcacg gcggcccccg
     1201 cccggtattc cgtgggcagg gggtcgccct ggttccgata ggtcaatttc ggaccggcgt
     1261 tcgtcacggc cccgcccagg gttaaatttc tggaaacgtc ccattgaaat ccggcgtccc
     1321 ccgcgaaaac cgcggcgtgg tagtcgcgaa ccaacgtgga ggaaagcgcc ttcccggcga
     1381 cccccaaccg cagactttcc cccagggcga ttccccccgt gagggccccc acccaatcct
     1441 gttgagcccg gcgcgtgtat cccgggccgc tcagaggcac cacgtcgatc tttccggcgt
     1501 cgaaaaacaa acccgtggcg gcccacccgg cccgaccgtt tccccggccg aagctggcca
     1561 ttccggttcg aacgtcgccc ggcgcggatt caaattgggt cgacgcctcc gcgcccacca
     1621 gggtgggcag ggcggcgggg ttcacggcgg ccacgcccag gtcccccgtg agggccgtgg
     1681 cggcccccga gaggccggtc acccgggcgt cggcggtctc caacaaggaa ttcccggccg
     1741 tccggggatc ggaagccccc cagagcccgg tgaatccgaa agcgaggaaa agagtcgaaa
     1801 gagcggcgcg tcgcatgtta tttcaccacc gccattttta agatttgctt cagattatcg
     1861 gtttcgaacc gcaccagata aattccgctg gccacgatgt ctccgtcgga ggcccggccg
     1921 tcccaaacca cgctgtaatt gccggccgac ttgtcctcgt ccaacaacgt tttcaccggc
     1981 cgcccctgaa gcgtgtacac cccggcgaaa acgtgcccgg gcaacacgga gaaattgagg
     2041 gtcgccgatt cccccgccga cggatcgaag agattgccca ccgcgctgac ggccacgggc
     2101 ccgcctttga aattctggag ggattggctg gactgcaaaa cgctgtagga ccgggcgttc
     2161 ggttggaacg agtatccggt caaggacggg gtcaacgagt aggtgccctc gaaaagatgg
     2221 tcggccgaat aggcccccgt cgcggtggag atggaaaccc ccgtggaggc gatgttgccc
     2281 gccagggaca gcgtcacccc gggaatgggc gcaaaggaaa aatccaacac cgttccggaa
     2341 agggacaaac cccggatggc gtcggtcaca aatttggctt cggaaaggtc cgcgttcagg
     2401 taacccgcca aatagtgatc gaaccggttg aagaccacgg aggcgcccac gccaaccacc
     2461 gctcccgcgt caagaacgtt gggcgcccag gacccggcgg ccgtcgccgt ttttaatccg
     2521 gcggtggaat aataaacgat ccggggatcg tccgcggaat ccacggcgat gtcgcagaaa
     2581 aaccccgttc cggatcccga atccacgacc acggccgcga cggtgcttcc cgcggtgtcg
     2641 aattcgatgt agtccaaccc gtcgctgaag gtgtcgtaga cggccaagtg cgcccgcccc
     2701 aggctgtcca gggcgagggc gagcccgccc agcgtcgtcg tgtccgtccc gatgacgtag
     2761 ggggtggtga tcgcaaaagt ggaagcgccg gtttggcggg cgtaggacag ttcccggtaa
     2821 ctcgtgacgg tcgaaccgtt caccagggcg attcgggcgc tgtcggtgga ggtgagggcg
     2881 atgtccaccg gcccgccgaa ggtgctggtg agcacggtga aggtgctcca aacgccggcg
     2941 cttcgcgcgc cgaagaccgt ggccgaggaa acgctgttgt agtaagccac ccggggattt
     3001 cgatcggctc ccacggcgat ggaaacgaag gtgttcgttc ccgtaaacgc ctcgaccgta
     3061 tcggtggacc aggacccgcc gctcaagcgg ccgtgcatca ccccgggggt gccgctcgtc
     3121 tggtaataca cgacgtgggg gagcaatccg ttctccaggg ccaaagcgca ttggggaccg
     3181 acctgggccg ccgacacgct ggagagggcc caggtggtcg tgccgttggc cagttgggcg
     3241 tatttcaagg tcagggtggc ggtgtccacg aaaacgaggt gcacgttgtt ccgggtgtcg
     3301 atggccacgc cggtctgcat gaacaccgag gacgacacgg tcacggaggt ggacaccgtg
     3361 tcggtggaca ccgccgccgc ggccggcgcg gcggccaggg ccagtcccag gagaagagcc
     3421 gctttgttca tgcccgcgcc ggctccttca tcaggcccag aaaccggtcg aacaagtaat
     3481 gggcgtcgtg gggccccggg gcggcctcgg ggtggtattg gacggagaag agcggcaggt
     3541 gtttgtgccg gaggccttcc agggtgttgt cgttcaaatt gatgtgggtg agctccacgt
     3601 cgcctttggc cagggaatcg atgtccacca cgaacccgtg gttttgggtc gtgacttcca
     3661 ccttgcccgt ggtcaaatcc ttcacgggat ggttggcgcc ccggtggccg aatttgagtt
     3721 tgtaggtctt cccgcccagg gccaggccga gcaactggtg gcccaggcag atcccgaaaa
     3781 ccggaatcgg cgcggccgcg ccttcgttgt ggcggatcaa ccccttgagc gtgtcgatgg
     3841 cgtaggtcac cgcggccggg tcccccgggc cgttcgacag catcaccccg tcgggtttaa
     3901 aagcgagcag gtcggccagg gaggtcgtcg ccgggaaaac cgtcacgtcg cacccgaggg
     3961 ccgccaggga gttcataatg ttgtatttga cgccgaagtc catcaaggcc acgcgccggg
     4021 ccgccccgtt cccgggcttt ccgggagtcc aggcgtaagg tttggcgcag gacacttccc
     4081 gcaccaaatc ggccccgacc atggcggcgg attttttggc ccgctccacc agggctttgg
     4141 gcttttccgt cgacgtcgaa acaattcccc gacaggcgcc cacctcccga aggtggcgca
     4201 ccagggcccg ggtgtccaga ccgtcgatgc cgatcacccc gtgggttttc atgtagcctt
     4261 ccaaatcggc ggtcgatccc caatggctgg cccgggggct gaattcccgg gcgacgaagc
     4321 cggccacgtg gggttttttg gatt
//
LOCUS       DAOQLS010000093         4307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9933_length_4307_cov_4.099953, whole genome shotgun sequence.
ACCESSION   DAOQLS010000093 DAOQLS010000000
VERSION     DAOQLS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9933_length_4307_cov_4.099953"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PK362_02615"
     CDS             <1..1147
                     /locus_tag="PK362_02615"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNC73767.1"
                     /translation="SKSDRPFRMPDQAIYKFVSQGDHRRLVAGRIEAGRVRVGDKVVF
                     TPSNKVSTVKSIEAFNAAPRTEIDAGWSTGFTLTEEIYITRGEIMSHEAHPPQVSTRF
                     RANLIWLGKKPLEKNKDYKLKIHTQAVPVRLREIKKVIDASTVGSTALAKDRVDRHDV
                     ADVILETPRPIAFDEIAQGEATGRFVIVDGYDIAGGGIIAAAERDDQDEFRAEARLRD
                     FNWIRGEVTAEARARRFGHGAALVMFVGAAGAGKHQYARRVEKSLFDQGRSAYLLDGT
                     NVLLGVDSDLIFRQSTQQELVRRFAEVAHLLLDAGLVVVSTTNAIGLADVAEVQALIP
                     DFPVLVVEVGAASALSAADLRLKGTETEDAVAASVAELLARRLTPPA"
     gene            complement(1157..3457)
                     /locus_tag="PK362_02620"
     CDS             complement(1157..3457)
                     /locus_tag="PK362_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNC73768.1"
                     /translation="MTTDLAKDPVCGMTVDPRRNPPRVDHQGRSYFFCNPRCADRFRA
                     DPLRFLTPRPTPPADPRAIYTCPMDPEVRNVGPGTCPICGMALEPLRAADLLADDNAD
                     NRDMEKRFGAALLLTVPVFLASMAEMIPRVPFHRFLESPAARWAQFFLSVPVVLWAGA
                     PLWARAARSVLQRHLNMFTLIGLGLGAAYGQSVLALLRPQWIPASLRGHDGAAPLYFE
                     AAAVITTLVLLGQVLEGRARGKTRGALRTLLALTPKTARRVDDQGNGEDVPLDELRTG
                     DRLRVRPGEKIPVDGVVLDGLSAVDESLVTGESLPVEKRPGDRVTGGTLNGRGGLLIR
                     AERVGAETLLAQIVQRVAEAQRSRAPIQRLADRVASYFVPAVVGVALLAFGAWSLWGP
                     EPRLAHGLVNAIAVLIIACPCALGLATPMSIVVGTGRGALSGILIRNAEALEIFEKVD
                     TLVLDKTGTLTEGKPRVLSVIPLEGFNEESLLRLAAGLEQSSEHPLAGAVVAAALEKS
                     QRPGRAEGFQSFPGRGVAGRIEGKDVALGNAAFLNERGIDTGALTARAEGLHGEGQTV
                     LWVAVDGRAAGLLGAADAVKETTPAALAGLRREGLRLVMLTGDTRETAQAVARALGID
                     EVIAGVLPNQKGDVVQRLQAEGRRVAMAGDGVNDAPALALASVGIAMGTGADIAMESA
                     AITLVKGDLRGIARARRLSRATMGNIRQNLFFAFVYNLLGVPIAAGALYPFFGWVLSP
                     MLAAVAMTFSSVSVIANALRLRRGSL"
     gene            complement(3513..>4307)
                     /locus_tag="PK362_02625"
     CDS             complement(3513..>4307)
                     /locus_tag="PK362_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNC73769.1"
                     /translation="LGVGFDPGVVNAYCALAVKKHFDTIDTIDILDVNAGSHGKYFAT
                     NFDPEINFREFKKVWTWIDRQWVCKPVHAEKWTYDFPLVGPRTVYLTGHDELHSLSKN
                     INARSIRFWMGFGDHYINVFNVLKNIGMLSEAPVKTAEGLEVVPLKVLKAVLPDPASL
                     APNYTGWTCIGNLVKGTKNGQPKEILIYNTCDHAACYKEVESQAISYTAGVPPVAAAL
                     LVAQGVWNPQTMVNVEELDPEPFLALLDRIGLPTQIEDRTPAPAKA"
BASE COUNT          707 a         1407 c         1502 g          691 t
ORIGIN      
        1 gtccaaaagc gaccggccct tccgcatgcc cgatcaggcc atttacaaat tcgtcagtca
       61 gggcgaccac cggcgtttgg tcgccgggcg catcgaggcg ggccgggtgc gggtggggga
      121 caaggtggtg ttcaccccgt ccaacaaggt gagcaccgtc aaaagcatcg aggcctttaa
      181 cgccgcgccc cggaccgaga tcgacgccgg gtggtccacc gggttcacgc tgaccgagga
      241 gatctacatc acccggggcg aaatcatgag ccacgaggcc cacccgcccc aggtgagcac
      301 gcggttccgc gccaacctga tctggctggg caagaagccg ctggagaaga acaaggacta
      361 caaactcaag atccacaccc aggcggtgcc ggtccgcctt cgggaaatca aaaaagtgat
      421 tgatgcttcg accgtcgggt ccacggcgtt ggccaaggac cgggtggacc gccacgacgt
      481 ggcggacgtg attttggaaa ccccgcggcc catcgccttc gacgagatcg cccaggggga
      541 agccaccggg cggttcgtga tcgtggacgg ctacgacatc gccgggggcg gcatcatcgc
      601 cgccgccgaa cgggacgacc aggacgaatt ccgcgccgag gcgcgcctgc gggatttcaa
      661 ctggattcgg ggcgaggtga cggccgaggc ccgggcccgg cggttcggcc acggggcggc
      721 gctggtcatg ttcgtgggcg ccgccggggc ggggaaacac caatacgccc ggcgggtgga
      781 aaaatccctt tttgaccagg gccggtcggc ctatctgttg gacggaacga acgtgctgtt
      841 gggcgtggac agcgatttga ttttccggca gtcgacgcaa caggagttgg tgcggcgttt
      901 cgccgaggtg gcccacctgc ttctggacgc ggggctcgtc gtcgtctcca ccaccaacgc
      961 catcggcctg gcggacgtgg ccgaggtgca ggccctgatt cccgattttc ccgtgctggt
     1021 ggtggaagtg ggggccgcgt cggcgctatc ggcggcggac cttcgtttaa agggcaccga
     1081 aaccgaggac gcggtggccg cgtccgtggc cgagctgctc gcccgccgcc tgacgccccc
     1141 cgcctaacgc ttccctctaa agggaacccc gccgaagccg cagggcgttg gcgatgaccg
     1201 agacggaact gaaggtcatg gccaccgccg ccagcatggg gctcaagacc caaccgaaaa
     1261 acggatagag cgcccccgcc gcgatcggca cccccaacaa attgtagacg aaggcgaaaa
     1321 acaggttctg ccggatgttc cccatggtcg cccggctgag ccgccgcgcc cgggcgatcc
     1381 cccgcagatc ccctttgacg agggtgatgg ccgcgctttc catggcgatg tcggcgccgg
     1441 ttcccatggc gatgccgacc gacgccaggg ccagggccgg ggcgtcgttg accccgtcgc
     1501 ccgccatggc cacccggcgg ccttcggctt ggaggcgctg gacgacgtcg cccttctggt
     1561 tcggcaacac gcccgcgatg acttcgtcga tccccagggc ccgggccacc gcctgggccg
     1621 tttcccgggt gtcgcccgtg agcattacca gcctcagccc ttcccgacgc aaaccggcga
     1681 gcgccgcggg cgtggtttcc ttcaccgcgt cggccgcgcc caacaacccc gccgcccgtc
     1741 cgtccaccgc cacccagaga accgtttgtc cctccccgtg aaggccctcc gcccgggccg
     1801 tcagggcccc ggtgtcgatg ccgcgctcgt ttaagaatgc cgcgttcccc aaggccacgt
     1861 ctttgccctc aatccgtccc gccacgcccc gtcccggaaa ggattggaac ccctcggccc
     1921 gtccgggccg ttggctcttt tccagggcgg cggccacgac cgcgcccgcc aggggatgct
     1981 cgctcgattg ctccaggccg gccgccaggc ggagaagact ttcctcgttg aatccctcca
     2041 ggggaatcac ggagagcacc cggggttttc cctcggtgag cgtgcccgtc ttgtccagga
     2101 ccagcgtgtc caccttctcg aaaatttcca gggcttcggc gtttcggatg aggatgccgc
     2161 tcagggcgcc gcggccggtt ccgaccacga tggacatggg cgtggccaac cccagggcgc
     2221 aggggcaggc gatgatcagg acggcgatgg cgttcaccag tccgtgggcc aagcggggtt
     2281 ccggccccca cagggaccag gcgccgaagg ccaggagggc gacgcccacc accgcgggaa
     2341 caaaatagga cgcgacgcgg tcggccagcc gttggatggg ggcccgggac cgctgggcct
     2401 ccgccacccg ctggacgatc tgcgccaaaa gcgtttcggc gcccacccgt tccgcccgga
     2461 tgagaaggcc gccgcgaccg ttcagggtgc cgccggtgac ccgatccccg gggcgttttt
     2521 ccacgggcag ggattcgccc gtgaccaggg attcgtccac cgcgctcaag ccgtccagca
     2581 ccacgccgtc cacgggaatt ttttccccgg ggcggacccg caggcggtcg cccgtccgaa
     2641 gttcgtccaa aggcacgtcc tcgccgttcc cttggtcgtc cacccggcgg gccgttttgg
     2701 gggtcagggc caggagcgtg cgcagggcgc cccgggtttt cccccgggcg cggccctcca
     2761 gcacctgccc cagaagcacc aaggtggtga tgacggcggc ggcttcgaaa tagaggggcg
     2821 ccgccccgtc gtggccccgc agggacgcgg ggatccactg ggggcggagc agggcgagga
     2881 cgctttggcc gtaggcggcg cccaggccca ggccgatcaa ggtgaacatg ttgaggtggc
     2941 gctggaggac ggaccgggcg gcccgcgccc acagcggggc gccggcccaa agcaccacgg
     3001 ggacgctcag gaaaaattgg gcccaacggg cggcggggct ttcgagaaaa cggtggaagg
     3061 ggacccgggg gatcatttcg gccatggagg ccaggaacac cggcaccgtc aaaaggagcg
     3121 cggcgccgaa tcgcttctcc atgtcccggt tgtcggcgtt gtcgtcggcg aggaggtcgg
     3181 ccgcccgcag gggttccagg gccattccgc aaatcgggca ggtccccggt ccgacgttcc
     3241 gcacttccgg gtccatgggg caggtgtaga tcgcccgggg gtccgccggc ggggtgggcc
     3301 ggggcgtcaa aaaccggagc ggatcggcgc ggaagcggtc ggcgcagcgc ggattgcaga
     3361 aaaaatagga acggccctgg tggtccaccc ggggcgggtt ccggcggggg tccaccgtca
     3421 tgccgcagac ggggtctttg gcgaggtcgg tggtcatggg gtcgggaagg gggaccatcc
     3481 gccggaggtc gggccgacgg cgcccccggg ggttacgcct tcgccggagc cggcgtccgg
     3541 tcttcgattt gcgtcggaag gccgatgcgg tccaacaggg cgaggaaggg ttcggggtcg
     3601 agctcttcca cgttcaccat ggtttgcggg ttccagacgc cctgggccac cagaagggcc
     3661 gccgccacgg gcggcacgcc ggccgtgtag gaaatggcct gggattccac ttccttgtag
     3721 caagccgcgt ggtcgcaggt gttgtaaatc aaaatttcct tgggttggcc gtttttggtc
     3781 cctttgacga ggttgccgat gcaggtccac ccggtgtagt tcggcgccag ggaggccggg
     3841 tccggcagga cggccttcag cacttttagg ggaaccacct cgagcccttc ggcggttttg
     3901 accggcgcct ccgagagcat gccgatgttt ttcaacacgt tgaacacgtt gatgtagtgg
     3961 tcgccgaagc ccatccagaa acggatggag cgggcgttga tgttcttgga cagggaatgc
     4021 agttcgtcgt ggcccgtgag gtacacggtc cgggggccca ccagggggaa gtcgtaggtc
     4081 catttttcgg cgtggacggg tttgcacacc cattgccggt cgatccaggt ccaaaccttc
     4141 ttgaactcgc ggaaattgat ttccggatcg aagttggtgg cgaaatattt gccgtggctt
     4201 ccggcgttga cgtccaggat gtcgatggtg tcgatggtgt cgaaatgttt cttgaccgcc
     4261 agggcgcagt aggcgttcac cacgccgggg tcgaagccca cgcccag
//
LOCUS       DAOQLS010000094         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_9970_length_4299_cov_4.180961, whole genome shotgun sequence.
ACCESSION   DAOQLS010000094 DAOQLS010000000
VERSION     DAOQLS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9970_length_4299_cov_4.180961"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PK362_02630"
     CDS             complement(<1..129)
                     /locus_tag="PK362_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNC73770.1"
                     /translation="MPRPPFPRLAPSILSADFAHLADDVRKVESAGADWLHVDVMDG"
     gene            complement(129..1178)
                     /locus_tag="PK362_02635"
     CDS             complement(129..1178)
                     /locus_tag="PK362_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNC73771.1"
                     /translation="MTDPTSYSGNPSDGGLPPGRLESLASRVGPSAEAVRRHPWAFLA
                     AAFLAAAVSTALASYWIIGGLIHSRQAVTVPDLTGKNLETALDLLGPLRLSLAKDAVQ
                     IDENFPPGAILRQVPPAGIQVREGKVVHVTLSSGGQVLFVPELTGTTLTEAQNRLRSA
                     GLVLGAMSQAYSTQKEAGWVMEQTPAVGAVIKPGSMVDLKVSKGPPPEGTTLMPDFIN
                     RPFGLARQWAKDAGVEVTSTEELSATLPGLVLSQTPAPDTPLGEKAVATFLVAKSSST
                     AAADVKLIRYEIPGGTERVQVRIVLRDDDGEKEVYQSQQNAGSVVEVAVAPKGAARAR
                     IFVDGVLIEERPLLP"
     gene            complement(1232..2296)
                     /gene="rlmN"
                     /locus_tag="PK362_02640"
     CDS             complement(1232..2296)
                     /gene="rlmN"
                     /locus_tag="PK362_02640"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNC73772.1"
                     /translation="MTDSPTALLDVPFDRWAATLGLEGAQLFRARQVQTWVFEKRAAA
                     FSEMTDLPADLRTRWESAFRLRTAAFLRQDTSAEDGTARLFFKAADGRDFSTVYLPAR
                     KETEDRASLCLSTQVGCAWGCVFCASGRVAYERNLSPSEILEQIFGAEKATGRRAQSL
                     LFMGMGEPLANLDNLRIALGAIRSPLGLNIGARHVTVSTSGLVPAIRKLAQEGPRVNL
                     AISLHAADDETRRKVLPKSSAWSIKDLLSAAWDYQKLAGGGGRVTFEYILLAGINDSI
                     REAQRLSNLLRGKKAWVNLIAYNPVAGLPYRRPDDETVRAFAKVLEDRGLFVRLRKPQ
                     GTDIEAGCGQLGRPRSLPPA"
     gene            complement(2331..3218)
                     /gene="htpX"
                     /locus_tag="PK362_02645"
     CDS             complement(2331..3218)
                     /gene="htpX"
                     /locus_tag="PK362_02645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNC73773.1"
                     /translation="MAFMRRIVLFMAVNLLVVTTISIVLQLLGVHGYMTARGLDYQAL
                     MVFCLVWGLFGALTSLALSRIMAKWMMGVRVIDPAQATGDAAWLVQTVHALARAADLP
                     EMPEVGIYASPELNAFATGPTKSRALVAVSAGLLESMDRRQVEGVLGHEITHISNGDM
                     VTMTLIQGVVNAFVMFFSRVIGFLVSQQVKEEARRSIQFLVTIVSQILFSLLGMIVVA
                     SFSRWREYRADAGGARLAGREKMIAALEALGASRAPVDTRQASVAALKISGRRGFLAL
                     LSTHPPLEDRIARLNRADY"
     gene            complement(3234..4160)
                     /gene="fmt"
                     /locus_tag="PK362_02650"
     CDS             complement(3234..4160)
                     /gene="fmt"
                     /locus_tag="PK362_02650"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNC73774.1"
                     /translation="MLKSVFFGTPSVAVPFLERLAKTTRVVGVVASPDKPAGRGYQLA
                     APPVKIAAEKLGLPVHQPPSLKTFSLRAALGETPDLGIVVAYGHLIPPAVFNAPRFGL
                     INAHFSLVPRYRGAGPMQWALIRGETETGVSLFRIDQGLDTGPVFLQRAAPIFPSDNA
                     ATLRERLVALGVELQDELIRKLEEGPVEPIPQSGEVSQAPLLKKEDGEIFWDRHTADQ
                     VANLVRGTYEWPGAFARMKGQRIKIRAAEPRPGGNGRPGEVIAVEKGRGFLVKCLSDS
                     VRVTRVQPDGKKEMDAESFWNGARLSVGDTFE"
BASE COUNT          783 a         1354 c         1457 g          705 t
ORIGIN      
        1 gccgtccatg acgtcgacgt gaagccaatc ggcgccggcg gattccactt ttcgaacgtc
       61 gtccgccaga tgggcgaaat cggccgaaag gatggacggg gccaggcggg ggaacggcgg
      121 acggggcatc aggggaggag gggccgctct tcgatcaaaa cgccgtcgac gaagatgcgg
      181 gcccgggccg cgcccttggg cgccacggcc acttccacga cggaacccgc gttttgctgg
      241 gattggtaaa cctctttttc gccgtcgtcg tcccgaagca cgatgcgcac ctggacgcgc
      301 tcggtgccgc cggggatttc gtagcggatc aatttcacgt cggccgcggc ggtggagctc
      361 gacttggcca ccaggaaggt cgcgacggct ttttccccga gcggcgtgtc gggggccggg
      421 gtttggctca acaccaaccc cggcagggtg gccgacaact cttccgtcga tgtaacctcc
      481 acgccggcgt ctttggccca ttggcgcgcc agcccgaagg gccggttgat gaaatcgggc
      541 atgagggtcg tgccttcggg gggcggcccc ttggacacct tcaagtccac catggacccc
      601 ggtttaatga cggcaccgac ggcgggcgtt tgttccatca cccacccggc ttccttttgg
      661 gtcgaatagg cctgggacat ggcgcccagg accaggcccg cggaacggag ccggttttgg
      721 gcttcggtca acgtcgtgcc cgtcagttcg ggcacgaaca acacctggcc gccggaggac
      781 aacgtcacgt ggaccacttt cccctcgcgg acttgaatgc ccgcgggcgg aacctgccga
      841 aggatggccc cgggcggaaa attctcgtcg atttggacgg cgtctttggc cagggacaga
      901 cgaaggggcc ccagcaggtc cagggccgtc tccaggtttt tgcccgtcaa atccggcacg
      961 gtgaccgcct ggcggctgtg gatcaatccg ccgatgatcc aataggaggc cagggccgtg
     1021 gaaacggcgg ccgccaaaaa cgccgcggcc aaaaaggccc agggatggcg tcgcacggcc
     1081 tcggcggagg ggcccacgcg gctcgccagg gattcgaggc ggccgggcgg aagacccccg
     1141 tcggaggggt tgccggaata ggaggttgga tcggtcatgg tttggaatta agaaagaaaa
     1201 accatcttac catatcggcc gggggcgact ttcacgccgg gggcagactt cgggggcggc
     1261 ccaattggcc acaccccgcc tcgatgtcgg tgccctgggg tttccgcagt cgaacgaaaa
     1321 gtccccggtc ttcaagcact ttggcgaagg cgcgcacggt ttcatcgtcg ggccgccggt
     1381 agggcaaccc cgccacggga ttgtaggcga tcaaattgac ccaggccttt ttcccccgaa
     1441 gcaggttcga caaccgctgg gcctcgcgaa tggaatcgtt gatcccggcc aaaaggatgt
     1501 attcgaaggt gacgcggccg cctccgccgg ccagtttctg gtaatcccag gccgcgctca
     1561 aaaggtcttt gatggaccag gccgaacttt tgggcaggac tttccgccgg gtttcgtcgt
     1621 cggcggcgtg caggctgatg gccagattga cccggggccc ctcctgggcc agctttcgga
     1681 tcgccggcac gagccccgag gtggacacgg tcacgtgccg ggccccgatg ttgaggccca
     1741 ggggcgaccg gatggcgccc agcgcgattc gaagattgtc caggttggcc aggggttcgc
     1801 ccatgcccat gaaaagcagg ctctgggccc gacggccggt ggccttctcg gcgccgaaaa
     1861 tctgctccag gatttcggac ggggaaaggt tgcgctcgta ggccacccgg cccgaagcgc
     1921 aaaacacgca gccccaggcg catcccacct gggtggagag acagagggag gcccggtcct
     1981 cggtttcctt tcgggcgggg aggtagacgg tggagaaatc ccgtccgtcg gcggccttga
     2041 agaaaagccg ggcggttccg tcctcggcgg aggtgtcctg ccgaaggaag gccgccgtgc
     2101 gcaaacggaa ggcgctttcc caccgggtgc gaagatcggc gggcaaatcc gtcatttcgg
     2161 aaaaggcggc ggcgcgtttt tcgaacaccc aggtctgcac ctggcgggcc cgaaagagct
     2221 gggccccctc cagcccaagg gtcgcggccc accggtcgaa gggaacgtcg agaagggccg
     2281 tcggggaatc ggtcatggac gggcggtccc ccggcggggg agcgcggggc tcagtaatcc
     2341 gcccgattca ggcgggcgat gcggtcttcc aggggcgggt gggtggacaa caaggccagg
     2401 aagccccgcc gtccggaaat cttcaaggcg gccacagagg cctgtcgggt gtccacgggg
     2461 gcccgggacg ctcccagggc ctccagggcg gcgatcattt tttcccgccc ggccaggcgc
     2521 gcgccgccgg cgtcggcgcg gtattcccgc caccgggaaa aggacgccac cacgatcatg
     2581 cccaacaggc tgaataggat ctgagagacg atcgtgacca ggaattgaat gcttcggcgg
     2641 gcttcctcct tcacctgctg gctcacgagg aaaccgatca cccgggagaa gaacatgacg
     2701 aaggcgttga ccacgccctg aatcagcgtc atggtcacca tgtccccgtt ggaaatgtgg
     2761 gtgatttcgt ggccgaggac gccctccacc tgccgccggt ccatggactc cagcagaccc
     2821 gccgacaccg ccaccagggc ccggcttttg gtcggtcccg tggcaaaagc gttcaattcg
     2881 gggctggcgt agatgccgac ctcgggcatt tcgggcaggt ccgccgcccg ggccagggcg
     2941 tggacggttt ggaccagcca ggccgcgtcg ccggtggcct gggccgggtc gatgacccgg
     3001 acgcccatca tccatttggc catgattcgg gagagcgcca gggacgtgag ggcgccgaac
     3061 aggccccaaa ccaggcagaa gaccatcagg gcctggtaat ccaacccccg ggcggtcatg
     3121 tagccgtgga cccccaggag ttgcaggaca atggaaatcg tcgtcaccac cagcaggttg
     3181 acggccataa acaaaacgat gcgcctcatg aaagccatgg attcaatcct ccgttattcg
     3241 aacgtgtcgc cgacggacag ccgcgcgccg ttccaaaaac tttcggcgtc catttccttt
     3301 ttcccgtcgg gctgaacccg ggtcacccgg acgctgtccg acaggcattt taccaaaaat
     3361 ccgcgaccct tctcgacggc gatcacttcg ccggggcggc cgttcccgcc cggccggggc
     3421 tcggccgcgc ggattttgat ccgctggcct ttcatccggg cgaaggcccc gggccattcg
     3481 taagtgcccc ggactaaatt ggccacttga tcggcggtgt gacggtccca gaaaatttca
     3541 ccgtcttcct tttttaaaag cggcgcctgg ctgacctcgc cggactgggg gatcggttca
     3601 acggggcctt cctccaattt acggatcaac tcgtcctgaa gttcgacccc cagggccacc
     3661 aggcgttccc gcagcgtggc ggcgttgtcc gatggaaaaa tcggcgcggc ccgctgaagg
     3721 aacacggggc ccgtgtccag gccctggtcg atgcggaaga gggacacccc ggtttcggtc
     3781 tcgccccgga tgagggccca ttgcatggga ccggcccccc gataccgcgg gacgagggaa
     3841 aaatgggcgt tgatcagtcc gaaccggggg gcgttgaaga cggcgggcgg gatcaaatgg
     3901 ccgtaggcca cgacgatgcc caagtccggc gtttcgccca gggcggcccg gagggagaac
     3961 gtttttaggg acgggggctg atggaccgga aggcccagtt tttcggcggc gattttgacc
     4021 gggggggccg ccagctgata gccgcgcccc gcgggtttgt cgggactggc gaccaccccc
     4081 accacccggg tggttttggc gagccgctcc agaaagggca ccgccaccga cggggtgccg
     4141 aagaaaaccg atttaagcat caagtctcag gcggtgcgcc ggagataaag gtaacccatc
     4201 acgaacccca ggaccgccag gccgagacaa acccgggacg ccgttttcaa acgacccttg
     4261 gccgcctcgc cgaccgggta acggtgggcg agattgttg
//
LOCUS       DAOQLS010000095         4281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10036_length_4281_cov_3.408661, whole genome shotgun sequence.
ACCESSION   DAOQLS010000095 DAOQLS010000000
VERSION     DAOQLS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10036_length_4281_cov_3.408661"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PK362_02655"
     CDS             complement(<1..398)
                     /locus_tag="PK362_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNC73775.1"
                     /translation="MSRRLTELQKKIVPAKKYSLEEAVALVKQTAKAKFDETVEIHVR
                     LGVDPTKGEQKVRGTVALPHGTGKSKKVVVIAKGEKIKEAQTAGADEAGDADVIEKIS
                     KGWLDFDVLVCTPDAMKDLTKLAKILGPKG"
     gene            complement(488..922)
                     /gene="rplK"
                     /locus_tag="PK362_02660"
     CDS             complement(488..922)
                     /gene="rplK"
                     /locus_tag="PK362_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNC73776.1"
                     /translation="MPPQKKVKTQIKLQIPAGGANPAPPVGPALGQHGVNIMDFCKQF
                     NERTKTMEAGMTIPAVITVFEDRSFSFITKMPPVSALIKKAAGLAKASSEPNKTKVGK
                     LSRAQLEDIAKQKMPDLNAPNLEAAVQMVKGTARSMGVETAD"
     gene            complement(934..1464)
                     /gene="nusG"
                     /locus_tag="PK362_02665"
     CDS             complement(934..1464)
                     /gene="nusG"
                     /locus_tag="PK362_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNC73777.1"
                     /translation="MARAWYVIHTYSGHEDKVKASLEKTAAQNGLADKFHKLLIPTED
                     VVELRRNKRQVKKRKFFPGYVLVDMDVDNQTYWMVRNTAGVTGFLGGVKPTPLPESEI
                     SSLLQLTEAPADHKPRPAVLFDKGENIRIKEGPFRHFIGVVEEVNEDRAKLKVTVSIF
                     GRATPVELDYLQVEKL"
     gene            complement(1532..1711)
                     /gene="secE"
                     /locus_tag="PK362_02670"
     CDS             complement(1532..1711)
                     /gene="secE"
                     /locus_tag="PK362_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNC73778.1"
                     /translation="MGDLIKFLKESWNELKLVSWLTRNQMIASTWLVVLFVVVFAIYV
                     GLLDLIVAKAFSLFV"
     gene            1946..3004
                     /locus_tag="PK362_02675"
     CDS             1946..3004
                     /locus_tag="PK362_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73779.1"
                     /translation="MTVPSRFASCAVFLAVFARPVAGAPLTTRSSPGGLWAQSYINQG
                     DQRPFEIQPAFHFHNYGFTAGYNQRFLTLWIGGLSLARHNDHFEYPDTRVDTHARGLQ
                     PAARLYYDRGRFLASFTSSVMTYRSNVEQVLDGPGGAFTDARAQTLRSYQQAVYVQYN
                     FGSAVYPTFPRIYKLSARFPRRSRPTLRDLDGGPRADVDRVYPPLNDAGRTVLAPWAR
                     LTDRDDAVDGYTARGTTPATDYAVLPYHQRFRKGSLGLDAGYYIVPETVLLVVSGGWT
                     RELKRNHVQWESRLLSDPPGVTYHNEAPRAPANTYEFGGGLRVFTGWGVAARLMYRHV
                     QTQRFKSGTYSFNLEYGL"
     gene            3408..3758
                     /locus_tag="PK362_02680"
     CDS             3408..3758
                     /locus_tag="PK362_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC73780.1"
                     /translation="MNILKKIRSTEGLTMVEVLITAAVFGILALIIAKSFFNTHRLSL
                     NATDQMEAARLSELIINRMQSANYYHVLGADSSTKAGNCASTSAFVSGSAAYPTNYLV
                     QWATSGPPRPCWEP"
     gene            3716..>4281
                     /locus_tag="PK362_02685"
     CDS             3716..>4281
                     /locus_tag="PK362_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73781.1"
                     /translation="MGHLWATSPMLGTLNDIETQVIAAGFTRWRIQVQAVRRDSSDND
                     NDASYVDFIPFSDSNRDSHDEYDTQLVFQGCNGDNDFNDVCGSSPEFPDSHMKQVDVF
                     LYKGSKQYSRLGFLLSQEKLTGNQGISGESPLKFNVDDPWAGCRIYTRTAANQLYLDT
                     PPVAPDFFRYWDYTAIPAAKAWKDGARRV"
BASE COUNT          795 a         1324 c         1230 g          932 t
ORIGIN      
        1 aagcccttgg gcccgaggat cttggccagc ttcgtaaggt ccttcatggc gtcgggcgtg
       61 cagaccagca cgtcgaaatc gagccagcct ttggaaatct tttcgatcac gtcggcgtcc
      121 ccggcctcgt cggcgccggc ggtctgggct tccttgattt tctcgccctt ggcgatgacg
      181 acgacttttt tggattttcc cgtgccgtgg gggagggcca ccgtgccgcg gactttctgc
      241 tcgcccttgg tggggtcgac gcccaagcgc acgtggatct cgacggtttc gtcgaatttg
      301 gccttggcgg tctgcttcac caacgccacg gcttcttcca gggagtactt tttggcggga
      361 acgattttct tctgcaactc ggtcaggcga cggctcatgg ttttctcttt tctcccccgc
      421 gcggaacgct ccctcctccc cgtcgaacgg cggcggggcg aggtgtcggg gaggttggga
      481 ttttaaatca gtcggcggtt tccacgccca tgctgcgggc ggtgcccttg accatttgaa
      541 cggcggcttc caggttgggg gcgttcaaat ccggcatctt ctgcttcgcg atgtcctcca
      601 actgggcccg ggacagtttc cccactttgg tcttgttggg ttcggagctc gccttggcca
      661 atccggcggc cttcttgatg agcgccgaga ccggcggcat cttggtgatg aaggaaaagg
      721 accggtcttc gaacaccgtg atcacggcgg gaatggtcat cccggcttcc atggtcttgg
      781 tgcgctcgtt gaactgcttg cagaaatcca tgatgttgac gccgtgctgg cccaacgcgg
      841 ggcccacggg cggcgccggg ttcgccccgc cggccgggat ttggagtttg atttgggtct
      901 taactttttt ctgtgggggc atggggggcc tcgttaaagt ttttcaactt gcaggtagtc
      961 caattcgacg ggcgtcgccc ggccgaagat ggacaccgtg actttcaact tcgcgcggtc
     1021 ctcgttcact tcctcgacga cgccgatgaa gtggcggaac ggaccttcct tgatgcggat
     1081 gttttcgccc ttgtcgaaca ggaccgccgg ccggggcttg tggtccgcgg gggcttcggt
     1141 gagctggagc agggacgaaa tttccgattc cggcaggggc gtgggtttga cgccgcccaa
     1201 aaagcccgtc acgccggcgg tgttgcgcac catccaatag gtctggttgt ccacgtccat
     1261 gtccaccagg acgtagccgg ggaaaaattt tcgctttttc acctggcgct tgttgcgccg
     1321 cagctccacc acgtcttcgg tggggatcag cagcttgtga aatttatcgg ccaggccgtt
     1381 ttgggcggcc gttttttcga gggacgcttt gaccttgtct tcgtgtccgg agtaagtgtg
     1441 aatgacgtac caagctcgcg ccacattgat cctttctaac ggagaagtcg ggcctctggc
     1501 ccgccgttgt cccgaaagct ctgcgtcggg gctagacgaa caacgagaag gccttggcga
     1561 cgatcaagtc caagaggccc acgtagattg cgaacaccac cacgaacaac accacgagcc
     1621 aggtcgaggc gatcatttgg ttccgcgtca accaggaaac cagcttcagc tcgttccacg
     1681 attccttcag aaatttgatc agatcgccca ttgaacgtcc gctccttgat cctagccaat
     1741 aaacgaagaa aacccttcgt cccgaaccgc aaccgcttta aattggatgg gggtttatta
     1801 ggaaagcgag agaatattaa acaattcgga cgaaggcgtc aatacgttcc ggccgccttt
     1861 tacccccccc gcgcggggag gtcctttttc cgtcggaaat gctaggattt cccattcatc
     1921 gccgcttcaa aaaaggatcg aaccaatgac cgtcccctcc cgttttgcgt cgtgtgccgt
     1981 ttttctcgcg gtcttcgccc gacccgtcgc gggcgccccg ttgacgaccc ggtcctcccc
     2041 cggcggcctc tgggcccaat cctacatcaa ccagggcgac caacgcccct ttgaaatcca
     2101 gccggctttc cacttccaca actacgggtt caccgccggt tacaatcagc gattcttgac
     2161 cctttggatc ggcggcctgt ccctggcccg gcacaacgac catttcgaat accccgacac
     2221 gcgggtggac acccacgccc gggggctgca acccgccgcg cggttgtatt acgaccgcgg
     2281 ccgctttttg gcgtccttca ccagttcggt catgacctac cgaagcaacg tggaacaagt
     2341 gctggacggc cccggcgggg cctttaccga cgcgcgggcg caaaccctgc gctcttacca
     2401 gcaggcggtt tacgtgcaat acaatttcgg ttcggcggtc tatccgactt ttccccggat
     2461 ctacaaactg tcggcgcgct tcccccgccg gtcccggccg accctgcggg acttggacgg
     2521 cggcccccgc gccgatgtgg accgggtcta cccgcccttg aacgacgccg gtcgcacggt
     2581 gctggccccc tgggcccgtc tcacggaccg ggacgacgcg gtcgacgggt acaccgcgcg
     2641 cggaaccacc cccgccaccg actacgccgt gttgccctat catcaacggt ttcgaaaagg
     2701 ttccctgggc ctggacgccg ggtattacat cgttcccgaa accgttcttt tggtcgtgag
     2761 cggcggctgg acccgggaat tgaagcgaaa ccacgtccag tgggaaagcc gcttgttgtc
     2821 cgatccgccc ggggtgacct accacaacga agccccgcgc gcgccggcca acacctacga
     2881 gttcggcggc gggcttcggg tgttcaccgg ctggggcgtg gccgcccgtt tgatgtaccg
     2941 ccacgtccaa acccaacgat ttaaaagcgg cacctactcc ttcaacctcg aatacgggct
     3001 ttgagggagt gggttctttc ccgcgtgaaa cttcgcaccg ggagccggcc cagcttccct
     3061 acgttccttt cccccctccg gtgggagtga ccgggggggg caatcggggc gtcgaccggg
     3121 gaccgacgaa atggctcctc cacgcggcct gattttcggc ggttcagtga aaaggacgcg
     3181 tgggccaacc aacacgcgta atcccagaat tggccatttt ggcgcatgac atcagggaaa
     3241 ggggcgctgg caaatgggaa tctttgaggc cctgtttttt ttgggaggaa taacattggt
     3301 tcccatcgaa aacgcggtag atttgtgaaa ggggggggct tgagctcacc gatccgcggc
     3361 actatacttg ggtgaaacgt ccatgcgaac gaaccaaaag ggataacatg aatattctca
     3421 aaaagattcg ttcaaccgaa ggactgacca tggtcgaagt tttgatcacg gccgccgttt
     3481 ttgggatttt ggccctgatc atcgccaagt cttttttcaa cacccatcga ttgtcgctca
     3541 acgcgaccga tcaaatggag gcggcccgcc tttcggaatt aatcatcaac cgcatgcaat
     3601 cggcaaacta ttaccacgtt ttgggagccg attcgagcac caaggccggg aactgcgcct
     3661 cgacttccgc cttcgtctcc gggagcgccg cctatccgac gaattacctg gtccaatggg
     3721 ccacctctgg gccacctcgc ccatgttggg aaccctgaac gacatcgaga cccaagtgat
     3781 tgccgcgggg tttacccgct ggagaatcca ggttcaagcc gtccgccggg actcctccga
     3841 caacgacaac gacgcctcct acgttgattt catccccttt tccgactcca accgagattc
     3901 ccacgacgaa tacgacactc aattagtgtt ccagggttgc aacggggaca acgattttaa
     3961 cgacgtgtgc gggagttcgc ccgagtttcc ggactcccac atgaagcagg tcgatgtgtt
     4021 tctttacaaa ggatcgaagc aatattcccg cctcgggttc cttttgtcac aggaaaaact
     4081 cacgggaaat caagggatct cgggggagag ccccctcaaa ttcaacgtcg acgatccctg
     4141 ggcgggttgc cggatttaca cccgaactgc cgccaaccag ttgtatctgg acacgccgcc
     4201 cgtggccccc gattttttcc gttactggga ctacacggcc atccccgcgg ccaaagcctg
     4261 gaaggacggc gcacggcgcg t
//
LOCUS       DAOQLS010000096         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10119_length_4262_cov_4.928928, whole genome shotgun sequence.
ACCESSION   DAOQLS010000096 DAOQLS010000000
VERSION     DAOQLS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10119_length_4262_cov_4.928928"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1129
                     /locus_tag="PK362_02690"
                     /pseudo
     CDS             227..1129
                     /locus_tag="PK362_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1493..2365
                     /locus_tag="PK362_02695"
     CDS             1493..2365
                     /locus_tag="PK362_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC73782.1"
                     /translation="MVGLLFTVPALVLLVTFLFLPIAVSFLMSLTDIDIFSINKWKDV
                     QFIGVSNYLRIVQDPVFWRSLWNTLYFAGLGVPLTIGVSLLAAVALNQKFVRGKSLFR
                     VALFAPVVTTVVAVAVVWRWLYNPEYGAFNWFLQNLGLKKLFWLSDSRTSLPSLIVMA
                     VWKNFGYNMVIFLAGLQTIPESQYEAARIDGASAWQSFRYITIPGLAPTLLFVLIMTT
                     IGYLQFFAEPYVMTRGGPLDSTMSIVLYMYNHGFRFFNLGYASAISYALFGLIFMFTF
                     VQMRLKKNGFEVFV"
     gene            2386..3207
                     /locus_tag="PK362_02700"
     CDS             2386..3207
                     /locus_tag="PK362_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNC73783.1"
                     /translation="MSDAVAAGGVHFLLAIAAAATLMPFFWMISTSFKNGGGLFTYPP
                     TWIPRDPTLQWYAQLVREVNFLVHFRNSVFVSVAITVLSLFVNSMAGYAFAKHRFRHR
                     DKIFSFLLATMMVPGQLTMIPVFLLLKKMGFLNSFLGLIVPVCANVFGIFLIRQFMMS
                     IPNDLIESARIDGCSEFRIYWSVVLPLCKPVLATLSIFTFIGSWNDFLWPLIVMVRED
                     GYTLPVALANLNGQHPTDFGLLMAAATVVVLPVLVVFLAAQKYVIRGIATTGLKE"
     gene            3250..>4262
                     /locus_tag="PK362_02705"
     CDS             3250..>4262
                     /locus_tag="PK362_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNC73784.1"
                     /translation="MNNLRKGTALALGLMMAWSPLPVLAKSKGAAPAAKKAAPEKKAA
                     AGGGVILDNFDRIDWASAKDAGSELKLSQAQGKNGSALKISYLMGTGKWVAINKSFSI
                     PSFRGKAISFWMKGKGAANNLEIKLVDEDDTNYGVKRPSLTTDGDWAQVTFTEADFSY
                     WWGGDQSLGAVKDLYIAVAAAGDGGSGEVLISDLRLVAANPAGALAKDGLVADGSSLE
                     SWTLAKGEGANATLFSGAGLKGKAVGVQYSIPANQWVAIRKTINADLSAGNAALVLRI
                     KGEGDPNNVEVKVVDRDESTFGKVFPGMGASNAWQEVRIPLSNMEYLWGGDNRLDATL
                     IRH"
BASE COUNT          751 a         1327 c         1265 g          919 t
ORIGIN      
        1 ctcttcgccg aacacaagat cacgcgtttc tcggaggccg gcctggagcc ccaaggcacc
       61 cccttcgacg ataattttac ccgaatcagt ttcgaagtcg tgttttagac cccgcatgcg
      121 ccgcgtttgg tggctcctcc ccctgctgtt ttcggtccgg tccgcccggg ccgaacggcc
      181 gttggtggtg tgggccatgg gcgaagaggg gaaaaagatc gccctcatgg cccgccggtt
      241 cgaggccgcc catcccgggg tcaccgtcga aacccaggcc attccttggg aagccgccca
      301 cgccaagctg ttgaccgccg tcgtgggggg cattccaccg gacgtgagcc aggtgggcac
      361 gacgtggatg gccgaattcg cggccatgga cgcgctcgaa cccctcgacg gccgcgccgc
      421 gtcatcgacc aacgcccgtc ccgacgcttt tttccccgga tcgcttcaaa cctgccgcgt
      481 ggacggcgcc ctctacggcg ttccttggta cgtcgacacc cgcgtgctct tttatcgaaa
      541 agatttgctg gcggccgtcg gttatccgga cggcccgtcc acctgggaag agctcacggc
      601 cgcggcccgg aaattgtcga cccggcgcac ggccgacggt cgacgggcct acggcatctc
      661 cctcggcgcc cgcagctgga ccgaccttct catggcggtg tggcagaacg gcggcgaccc
      721 cctgcgtccc acgtcgccgg ctttttttgg cgccgtccag cattacatcg ggttcttccg
      781 cgacaatctc acaccgacca aggaagcggc ggacgtggat cttttccacg ctttcaaaac
      841 cggctacttg cccatgttcg tttcggggcc ctggatgttg gagctgatcc ggaaagaact
      901 ccccgaattg gacggtcgct ggggggtggc aatgcttccg gggaaaaaat cccgcacctc
      961 cttcgtgggg ggatccaact tggttgtttt caaggcgtcg aaacaaaagg accgggcctg
     1021 gcagttcctt gaattcatga gcgatccggc caaccaggtc gagtggatga aaagcacgac
     1081 cgatctcccg tccgtgcagg tggcgtggaa agacccctat ttcagcgaca agcccctggt
     1141 gcaggtgttc ggccgccaac tgttcgacac cgcgagtccg ccgacggtgc ccgaatggga
     1201 gcagatcgcc agcgccatgg acgacaccat ggagcgcgtg gtcctcgatc ccgcccaaac
     1261 cgaggacacc atccgggtgg ccctggccga attggaaacc cgcattttga aactccggtc
     1321 ggcccgaacc cgggaaccgg gcagttggcg gcggttcggg cgatgggcgg gcgtggcggc
     1381 ccttctcctc gcggcggctc tctggttaac gacggtcgga cgcctccgtt cccgggccga
     1441 cggagccccg ggcgaagcgc ccttccagtt gcgggaattt ttccaacggg acatcgtcgg
     1501 tctgcttttc accgtccccg ccctggtgct cttggtgacg tttcttttcc tgcccatcgc
     1561 ggtgtccttc ctcatgagct tgaccgacat tgatattttc agcatcaaca agtggaagga
     1621 cgttcaattc atcggggtca gcaattacct gcgcatcgtc caggaccccg tgttctggcg
     1681 ttccctgtgg aacaccctgt atttcgccgg gctgggcgtc ccgttgacca tcggcgtctc
     1741 gttgttggcg gccgtggccc tgaaccagaa atttgtccgc ggcaaatccc ttttccgcgt
     1801 cgctttgttc gccccggtcg tgaccaccgt cgtggcggtg gcggtcgtgt ggcgatggct
     1861 ctacaacccc gaatacggcg ccttcaattg gtttttgcag aatctgggcc tcaaaaaact
     1921 tttctggctc tccgattccc gcacctccct gccgtccttg atcgtcatgg ccgtgtggaa
     1981 aaacttcggc tacaacatgg tcatcttttt ggccggcctt caaaccattc ccgaaagcca
     2041 atacgaggcg gcccggatcg acggggccag cgcctggcaa agtttccgct acatcaccat
     2101 cccggggttg gcgccgaccc tgctcttcgt cctcatcatg acgaccatcg gttatttgca
     2161 gttttttgcg gaaccctacg tcatgactcg gggggggccg ctggattcca ccatgtccat
     2221 cgtcctctac atgtacaacc acggctttcg gtttttcaat ttgggttacg cctcggccat
     2281 cagttacgcc ctcttcggcc tgatctttat gtttaccttc gtgcaaatgc gtcttaagaa
     2341 gaacggattc gaggtgtttg tttgaacgcc cggggcaaac gttggatgtc cgacgccgtt
     2401 gccgcggggg gcgtgcattt cctcctggcc atcgccgcgg cggccacgct gatgccgttt
     2461 ttttggatga tctccacgtc gttcaagaac ggcggggggc tttttaccta tccccccacc
     2521 tggattcccc gggacccgac ccttcaatgg tacgcccagt tggtgcggga ggtgaatttc
     2581 ctggtccatt tccgcaacag cgtgttcgtc tccgtggcca tcacggtcct gagcttgttc
     2641 gtcaattcca tggccggtta cgctttcgcc aagcaccgtt tccgccatcg ggacaaaata
     2701 tttagtttcc tcctggccac catgatggtt cccggccagt tgaccatgat ccccgttttc
     2761 ctgttgttga aaaagatggg tttcctgaac agttttcttg gtttgatcgt gccggtgtgc
     2821 gccaacgtct tcggtatttt cctgatccgc cagtttatga tgtcgatccc caacgatctc
     2881 atcgaatcgg cccgaatcga cggttgctcg gaatttcgga tttattggag cgtggtgctg
     2941 cccctgtgca agccggtcct ggccacgctg agcatcttca ccttcatcgg gtcctggaac
     3001 gattttctct ggcccctgat cgtcatggtg cgggaggacg gctacaccct cccggtcgcc
     3061 ctggccaatt tgaacggtca gcacccgacg gatttcggtc tcctcatggc ggccgcgacg
     3121 gttgtggtcc tgcccgtgct cgtggtcttt ttggcggccc agaagtacgt gatccgcggc
     3181 atcgccacca cgggcctcaa ggaataattt ttcatgcatt ctcaggaatc ttctcaaagg
     3241 agcggaacga tgaacaactt gcgaaaagga acggcgttgg cgctgggtct gatgatggcc
     3301 tggagccctc tgccggtcct ggcgaaaagc aaaggcgcgg ccccggcggc gaaaaaagcg
     3361 gcgcccgaga aaaaagcggc ggcgggcggc ggtgtgattc tggacaactt cgaccgcatc
     3421 gattgggcct cggccaagga cgccgggtcg gagctcaagc tctcccaagc ccaggggaaa
     3481 aacggttcgg ccctaaagat cagctacctc atgggcaccg gcaaatgggt ggccatcaat
     3541 aaatcgttct cgattccgag ctttcggggc aaggcgattt ccttctggat gaaaggcaaa
     3601 ggggccgcca acaacctcga aatcaagctg gtggacgaag acgacacgaa ctacggcgtt
     3661 aaacgtccct ccctgacgac cgacggcgac tgggcccagg tgaccttcac cgaagccgat
     3721 ttcagctatt ggtggggcgg ggatcaatcc ctgggcgcgg tgaaggacct ttacatcgcg
     3781 gtggccgcgg ccggcgacgg gggctcgggc gaagtgttga tttccgacct gcgcttggtg
     3841 gcggccaacc cggccggcgc cctggcgaag gacggtctcg tggcggacgg gtcctccctg
     3901 gaatcctgga ccctcgccaa gggcgaaggc gccaacgcca cgctcttttc gggagcgggg
     3961 ttgaagggga aagccgtcgg ggttcaatat tcgatcccgg ccaaccaatg ggtggccatc
     4021 cgtaaaacga tcaacgccga cctgtcggcg gggaacgccg ccttggtcct ccgcattaag
     4081 ggcgaaggcg atcccaacaa cgtcgaagtc aaagtggttg accgcgacga atcgaccttt
     4141 gggaaggtgt ttccgggcat gggcgcctcg aacgcctggc aggaagtccg catccccctg
     4201 tcgaacatgg aatacctttg gggcggggac aatcgcctgg acgccaccct gatccgccat
     4261 tt
//
LOCUS       DAOQLS010000097         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10365_length_4200_cov_4.070929, whole genome shotgun sequence.
ACCESSION   DAOQLS010000097 DAOQLS010000000
VERSION     DAOQLS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10365_length_4200_cov_4.070929"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..202
                     /locus_tag="PK362_02710"
                     /pseudo
     CDS             56..202
                     /locus_tag="PK362_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1011..2834)
                     /locus_tag="PK362_02715"
     CDS             complement(1011..2834)
                     /locus_tag="PK362_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNC73785.1"
                     /translation="MTRVDFVSFDPRRPGWNYSFRNPRASRTARRPEDVEGLLQWAED
                     QRKSGCWVALALAYEAAGALNPELPAGRSGPEPLAWAAAFPRPTAAVRPPRRRFSHTP
                     WRPGLTASTHHRSIARIKSWIARGDTYQVNHTFPLTSRFSGDARSWFWSLGPAQGAPY
                     AAFIDAGRWKWLSLSPELFFEKKGSRVVLRPMKGSAPRGRWPAEDDAFAERLARSPKD
                     RAENLMIVDLLRNDLGKIARPGTIRARPLFVVEKYPTVHQMTSTLSGRPRSGVGWREC
                     LAALFPSGSVTGAPKRRTMEIIRRLESTPRGFYTGAIGFLRPDGSGEFNVAIRTIVLD
                     GNTGRARCGVGAGVTWGSSAPGEWRESLLKGEFLNLPDFHLIESLRLEEGRFPLKEWH
                     WRRVRSSARRFGFVQDRKSFDRALARLAAGRSRGVWKARLLLGRDGRWTGSVESLPAA
                     AAPWRVALSTRAVDDRDPFLFHKTTRRSVYDAWAADRPGADEVVGWNRRGELTEGTRT
                     NVVLEIGGRLFTPPVSAGLLAGVYRGVLLGRGQIRERVLRKSDWRKADRVWLINAVRG
                     WIPARRHADSTVSRPKRVSAPRRRSRKPPRNGRRSNDPVRG"
     gene            complement(2850..3464)
                     /locus_tag="PK362_02720"
     CDS             complement(2850..3464)
                     /locus_tag="PK362_02720"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNC73786.1"
                     /translation="MSAVRITAAVSPSDPRVCAFTLDRSVSDRSVVCRAAAQAAGSPL
                     FEELFKLPGVVQVWADGPRVTVARGEDTDWKSFAGAVGAALRSALQSARPPVAAAVPA
                     NATGGDLAADVERVLAEEINPSLAGHGGRAELDGLEGRVARIRFVGGCQGCGAAQLTL
                     AHGVRRTLLDRLPALSDVRDVTDHAAGQRPYYPSGASGASPFPS"
     gene            complement(3461..>4200)
                     /locus_tag="PK362_02725"
     CDS             complement(3461..>4200)
                     /locus_tag="PK362_02725"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNC73787.1"
                     /translation="VQLSLAQQAPLTGCVVVTTPQDVALGVARKGLRMFEQVKVPVLG
                     IVENMSGFVCEKCGHDHKLFKKAGGETLARETGVPLLGAVPLEIAVMEGGDAGEPILL
                     RTDQSPAAKTFLEIARRLEAEVARQNAGTSAEEPTQAKIGPAGELKLTWPDGHEGSYA
                     AWTLRGRCPCAQCVDEDTGRRVLDPKRVPLDIRVTGVQPVGRYGLGLAFTDGHNTGIY
                     KFADFPALCECPACLARRGKPAETFSV"
BASE COUNT          677 a         1386 c         1409 g          728 t
ORIGIN      
        1 gggggccgcc gccctgctgg cggggatccc catcttttgg gaaaacggtg tcgaaatccg
       61 ccccgactcg cccgcggcgc ttctgtccgc cgcggccttt ctcgtccatt ttcgatcgat
      121 gaccaagggc gaccgacgcc ctcttttcct ttccggcgtg ctttggggtc tggggtggac
      181 gatcagcccg aaaatcactt ttccgttgat gggggtcgcc ctgtattggt ccgctctatt
      241 cttccgagaa cgggccgccc gggcggccac gcttcgggac gggctgagtc aggcggccgg
      301 ctttctcctg ccggttttgg tcctggcggc gggtttggcc cgggagggcg ccttggtcgg
      361 ttattggaat ttcgtgtggc gggggaacgc tttttggaac acgcgattca gcccgatgat
      421 ggtgatcgga tccatcgccc gggacaacac gcttctgctc gcggccggca ccgtcggtgt
      481 tttggcggcc ccgtggctct ttccggcgaa aaacgggaac cgcgccccgg ggcttctggc
      541 ctttctgggc tttggacttg ttttgggcct ctggataaac cccgtgccct acggacagag
      601 ctttttgttt ctcttcccct tctgggctct gggcgctttc ggggtctttc tgattttggt
      661 caaaagggcg gccgaacgtt ggggggcccg ggcggccgac ggtctgctgg ccgccctcct
      721 ggtggcgggg gttttcccgg ggcttttaaa gcgattgcaa cccttcgaac ggaatctccc
      781 ccaattgcaa ttattgacgg tcgtcaacga cgcggttccg ccggacgggt ccgtcctcga
      841 ttcttggacg gggttgggcg tgtttcgccc ccacgcgctt ttctacccgc acctgcaccg
      901 ggagatccga cgaaccctgc ccgcggattg gacggaacaa ttaaggaagg atttggtttc
      961 ggggcgtctc cgcccggccg ctttgttggg ccatcgggaa tgggcgggca tcaaccccga
     1021 actggatcgt tggatcgacg accattacgc ggcggtttcc gggaacgtcg tctgggtgcc
     1081 gaaaccctct tcggacggga aaccgtcgaa tccgcgtgcc gtcgggcggg gatccacccc
     1141 cgaacggcgt tgatcagcca cacccggtcg gctttccgcc aatcggattt tcgcaacaca
     1201 cgctcccgga tttggccccg tccgagcagc accccccggt aaaccccggc caacagcccc
     1261 gcggataccg gcggcgtgaa aagtcgcccc ccgatttcca aaaccacgtt cgtccgggtt
     1321 ccttcggtca gttccccccg gcggttccag cccacgactt cgtcggcgcc ggggcgatcg
     1381 gcggcccaag cgtcgtagac gctccgtctc gtggttttgt ggaacagaaa cgggtcgcga
     1441 tcgtccacgg cacgggtcga caaggcgacg cgccaggggg ccgcggccgc cgggaggctt
     1501 tcgacggaac cggtccaacg gccgtcccgg ccgagaagca ggcgggcttt ccacaccccg
     1561 cgggaacgcc ccgccgccag ccgagccagg gcccggtcga aagattttcg atcctggacg
     1621 aacccaaaac gccgcgcgga gcttcgaacg cgccgccagt gccattcctt taaaggaaac
     1681 cgaccttcct ccaagcgcag ggattcaatc aggtgaaaat ccggaaggtt taagaattcc
     1741 cctttcaaca gggattcccg ccattccccg ggggcggagg atccccaggt cacgcccgcg
     1801 ccgaccccgc accgggcccg tcccgtgttt ccgtccagga cgatggttcg aatggcgacg
     1861 ttgaattccc cgcttccgtc gggacggagg aacccgatgg ccccggtgta aaacccccgg
     1921 ggcgtggatt ccaaccgtcg gatgatttcc atggtgcggc gtttgggcgc gcccgtgacg
     1981 gacccgctgg ggaagagggc cgccaggcac tcccgccaac cgacgccgga acggggccgc
     2041 cccgaaaggg tcgatgtcat ctggtgcacg gtcgggtatt tttcgaccac gaagagcggg
     2101 cgggcgcgaa tcgttccggg gcgggcgatt ttccccaaat cgtttcgaag gaggtccacg
     2161 atcatcaggt tttcggcccg gtccttggga cttcgcgcga ggcgctcggc gaaggcgtcg
     2221 tcctcggcgg gccaacgccc tcggggggcc gaccctttca tgggacgcag aaccacgcgg
     2281 gagccttttt tttcaaaaaa cagttccggg gaaagactga gccacttcca ccgtcccgcg
     2341 tcgatgaagg cggcgtaggg tgccccctgg gcgggaccca gggaccaaaa ccagcttcgg
     2401 gcgtcgcccg aaaaacggga agtcaaaggg aacgtgtggt tcacctgata cgtgtcgccc
     2461 cgggcgatcc aagacttgat gcgggcaatc gatcgatggt gggtcgacgc tgtgaggccc
     2521 ggccgccagg gagtgtggga gaaacgccgt cgagggggac gaaccgcggc ggtgggacgc
     2581 gggaaggcgg cggcccaggc caggggctcc ggcccggagc gcccggcggg aagttcgggg
     2641 ttcaacgccc cggcggcttc gtaagccagg gccaatgcca cccaacaacc gcttttccgt
     2701 tggtcctcgg cccattgaag aaggccttcc acgtcttcgg ggcgccgggc cgttcgggac
     2761 gcccggggat tccgaaagga ataattccaa ccgggccgcc ggggatcaaa agaaacgaaa
     2821 tccacccggg tcatggggcg aggctcgggc taggagggaa aggggctggc gcccgaggcc
     2881 ccggaaggat aataaggccg ttggcccgcc gcgtggtcgg tgacgtcccg aacgtcggaa
     2941 agcgccggaa gccggtccag aagcgttcgg cgcacgccgt gggccagggt caattgggcg
     3001 gccccgcacc cctgacaacc gcccacaaaa cggatccgtg ccacccgacc ctccaacccg
     3061 tccaattccg ctcggccgcc gtggcccgcc agggagggat tgatttcttc cgccagcacc
     3121 cgctccacgt cggccgccag atccccgccg gtcgcgttgg ccgggacggc ggccgccacc
     3181 ggcggccggg cgctttggag ggccgaccgc aaggccgcgc ccaccgcgcc cgcgaaggat
     3241 ttccagtcgg tgtcctcgcc ccgggccacg gtcacccgcg ggccgtcggc ccacacctgc
     3301 acgaccccgg gcagtttgaa caattcctca aagagggggg accccgccgc ctgggcggcg
     3361 gcgcggcaca cgacggaacg gtcggagacg gagcgatcga gggtgaaggc gcacacccgc
     3421 gggtccgacg gcgacaccgc ggcggtgatg cggacggcgc tcaaacggaa aaagtttcgg
     3481 ccggtttgcc ccgacgggcc aggcaggccg ggcattcgca gagggccggg aaatcggcga
     3541 atttgtagat gcccgtgttg tggccgtcgg tgaaggccaa ccccaggccg taacggccga
     3601 cgggttgaac gccggtgacc cggatgtcca ggggaacgcg cttcggatcc aggacccggc
     3661 ggccggtgtc ctcgtccacg cactgggcgc aggggcatcg gccccgcagg gtccaggcgg
     3721 cgtaggaccc ctcgtggccg tcgggccagg tcaactttaa ttcgcccgcc gggccgattt
     3781 tggcctgggt cggttcctcg gcggaggttc cggcgttttg tcgagccact tccgcctcga
     3841 ggcgccgggc gatctcgaga aacgtcttgg cggcggggga ttggtcggtg cgcagcaaaa
     3901 tcggctcccc ggcgtctccc ccttccatga cggcgatctc caggggcacc gcgcccaaca
     3961 acggcacgcc ggtttcccgg gccagggttt cccccccggc ttttttgaat aatttgtggt
     4021 cgtggccgca tttttcgcag acgaacccgc tcatgttctc cacgatgccc aggaccggca
     4081 ctttcacctg ctcaaacatt cgaaggccct tgcgggccac gcccagggcc acgtcctggg
     4141 gcgtcgtcac gacgacgcac cccgtgaggg gcgcctgctg ggccagcgac aattggacgt
//
LOCUS       DAOQLS010000098         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10385_length_4194_cov_4.320367, whole genome shotgun sequence.
ACCESSION   DAOQLS010000098 DAOQLS010000000
VERSION     DAOQLS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10385_length_4194_cov_4.320367"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PK362_02730"
     CDS             <1..500
                     /locus_tag="PK362_02730"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNC73788.1"
                     /translation="WPLLITAPAALDADGFLLSAATLIGGLLCDGLGDAVQLDSGLGL
                     ERGLELASNVLQGAGARITKTEFVSCPSCGRTLFDLQTTTERIKKRTGHLKNVKIAIM
                     GCIVNGPGEMADADFGYVGGGPGKINLYVGKECVAKGIPFNEADERLVALIKENKKWT
                     EPHES"
     gene            490..1671
                     /locus_tag="PK362_02735"
     CDS             490..1671
                     /locus_tag="PK362_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNC73789.1"
                     /translation="MSPSLFLDAKGDSRPLIFRIFFFAAFGFLLFQLFRLLSPFFDGI
                     MVAITLALVFYPVHARFLKWTGGRRTLAAGLSVAALFLIVVIPTLIFLGMLGKQTAAL
                     YPWAQEQIQTLRANPQEAFINKLPASVRSAWERTDKVLDNAGVDIQDVLLRNIEGLGH
                     QLSATGAAVLKNLVFFIFQTFVMLFTLFFLFRDGARLLHQLVQLIPMESVHKEHILNR
                     LNQTLTAVVRGMFITASVQGLLAGIGFAVAGVRFSVLLGFATAFMALVPLVGATAVWA
                     PVCGYLILKGWMWPGILLLIWSAGVVSTIDNFLRPYLIGEKARLPIMLLFFGTLGGLK
                     AYGPTGIFFGPVMVASVLAFAKIYGEQLQRIQTAQDSPERGRRPSDAPSGPLPPTSPA
                     L"
     gene            1673..3085
                     /locus_tag="PK362_02740"
     CDS             1673..3085
                     /locus_tag="PK362_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNC73790.1"
                     /translation="MFKKNRRPFRGAKPPFDSGRGEGRNPSRDDRSPFDPNQRGPKKF
                     NHGDRFRPGKRNDKDRRPGRFGKPDSPSSFDPKFGGAPGEPGANAGRPPEFRGDRKRY
                     NPGKSKDFRRRPGRFDRADNRNNKPKFNTRYDRDRSSSSDGENRPLLPGQPDPRRPRP
                     FDEGAGAEPLPAPAPPPFEAPPLFRGTPGPEKPRPPSGEEPPPRPFFGRGEKINRTSP
                     IVHRFRDARDGKDPKSIFVEGHRLVADLIHIPLAVREFLFTVAAADRPEIRRVIDALR
                     GRGASGHNVTPDVMEFVSDLETPPGLVVRADRPVSPALPLADNALWVVLDGLQSPANA
                     GAVVRVAEAAGAQAVIAAAGTADLWSPKALRGSSGSAFRVPLVHIPDLKEVSNRLKGR
                     GIAAVAADQKADLDYWDYDWTRPTAILLGAEGRGLGDLSDFDAQRVRVPMAGKVESLN
                     VAVAAGLLLMEAARQRRSKS"
     gene            3088..>4194
                     /locus_tag="PK362_02745"
     CDS             3088..>4194
                     /locus_tag="PK362_02745"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNC73791.1"
                     /translation="MQTGLDLLSKRGHPALKGKRVGLIVHQASVDGQLRHAVDLLRNR
                     KDFRVAALFAPEHGFYGDLQDQVPVDGARDPRTGLPVFSLYGPRRQPTAPMLKGLDAL
                     VFDLQDIGVRYYTFIWTMALALEAAAKTGITFVVLDRPNPLGGAVEGNLPDPAYASFV
                     GLYPLPVRHGKTVGELAVYFNKKYQWGADLHVVKMKGWKRSMRFDETGLPWVMPSPNM
                     PTLDTATVYAGMCLLEATNLSEGRGTTRPFEIAGAPFIDGHRLARALEKKNLPGVAFR
                     PLAFRPTFNKWANQTCGGVQLHVTNFKTFKSFLTGLIFLQTVQELHPRRFKWNPPPYE
                     YETVKRPIDILCGTDRIRKSIDAGDNLFNLSKTWP"
BASE COUNT          731 a         1452 c         1221 g          790 t
ORIGIN      
        1 cgtggccttt gctgatcacg gctccggcgg ccctggacgc ggacggattt ctcctgtcgg
       61 ccgccacgct catcggcggc cttctttgcg acgggctggg ggacgccgtc caattggatt
      121 cgggactcgg cctggagcgc gggctggaac tcgcctccaa cgtgctccag ggcgccggcg
      181 cccgcatcac caagacggaa ttcgtttcct gcccctcctg cgggcgcacc cttttcgatt
      241 tgcagaccac cacggagcgg atcaaaaaac gcaccggcca cctgaagaac gtcaaaattg
      301 ccatcatggg gtgcatcgtc aacggccccg gcgaaatggc cgacgcggac ttcggctatg
      361 tcgggggcgg cccgggcaaa atcaatttgt acgtgggcaa ggaatgcgtg gccaagggca
      421 ttcccttcaa cgaagccgac gaacgcttgg tcgccctcat caaagaaaac aagaaatgga
      481 cggaacccca tgagtcctag cttattcctc gacgccaaag gcgattcccg acctttgatt
      541 ttccggattt tctttttcgc ggctttcggt ttcctgctgt tccagctttt ccgattgctg
      601 tcgccttttt tcgacggcat catggtcgcg atcaccctcg ccctggtgtt ttaccccgtc
      661 cacgcccggt tcctcaaatg gaccggcggc cgccgaaccc tggccgcggg gctgagcgtc
      721 gccgcccttt tcttgatcgt cgtcatcccg accctgatct ttttgggcat gctgggcaag
      781 caaaccgccg ccctctatcc ctgggcccag gaacagattc aaacccttcg ggccaatccc
      841 caggaagcct tcatcaacaa attgcccgcc tcggtccgct ccgcctggga acgcaccgac
      901 aaagtgctcg acaacgccgg cgtggacatt caagacgtcc tcctgcgcaa catcgaaggg
      961 ctgggccacc agctttccgc caccggcgcg gccgtcttga aaaatctcgt gtttttcatt
     1021 tttcaaacct ttgtcatgtt gttcacgctg ttcttccttt ttcgggacgg ggcgcgattg
     1081 cttcaccagc tcgtccagtt gatccccatg gaatcggtgc acaaagaaca catcttaaac
     1141 cggctgaacc agaccctcac ggcggtggtc cgggggatgt ttattacggc gtcggtccag
     1201 ggcctgctgg cggggatcgg cttcgcggtg gcgggcgtgc ggttttcggt tctgctggga
     1261 ttcgccacgg ctttcatggc cctggtcccc ctggtgggcg ccacggcggt gtgggcgccg
     1321 gtctgcgggt atttgatttt gaagggctgg atgtggccgg gcattcttct gttgatttgg
     1381 agcgccgggg tggtcagcac catcgacaat tttctccgcc cctatttgat tggagaaaaa
     1441 gcccgcttgc ccatcatgct cctgttcttc ggaaccctgg gcggcctgaa ggcctacggc
     1501 cccacgggca tctttttcgg tccggtcatg gtggccagcg tattggcctt cgccaagatt
     1561 tacggcgagc agctgcagcg cattcaaacc gcccaagatt ccccggagcg gggacgtcgg
     1621 ccctccgacg ccccgtccgg tccccttccg ccgacgtcgc cggccctctg acgtgtttaa
     1681 aaaaaaccgg cgtcccttcc gcggagccaa gccccccttc gattcgggcc ggggcgaggg
     1741 gcgaaacccc tcccgcgacg accgttcccc tttcgacccc aaccaacggg gccccaaaaa
     1801 gttcaaccac ggagatcgtt tccgtcccgg gaaacggaac gacaaagacc gccggcccgg
     1861 ccggtttggg aaacccgatt ccccctcctc ttttgatccg aaattcggcg gcgcgcccgg
     1921 ggaaccgggg gccaacgccg gccgcccccc ggaattccgg ggcgaccgaa agcgttacaa
     1981 ccccggcaaa tccaaggact ttcgtcgacg tccggggcga ttcgaccggg ccgacaaccg
     2041 caacaacaaa cccaaattca acacccgata cgaccgggat cgatcgagtt cttccgacgg
     2101 cgaaaatcgc cccctcctcc ctgggcaacc cgacccccgt cggccgagac ctttcgacga
     2161 aggcgccggg gccgaacccc ttcccgcgcc ggcgcccccg ccgttcgagg ccccgcccct
     2221 ttttcggggg accccgggcc cggagaagcc ccgcccgccg tcgggagagg aaccgccccc
     2281 ccgaccgttt ttcgggcgcg gcgaaaagat caatcgaacc agccccatcg ttcaccgatt
     2341 ccgggacgcc cgggacggca aggaccccaa atccattttc gtggagggcc accgcttggt
     2401 ggccgactta atccacatcc ccctggcggt tcgggaattt ctcttcaccg tggccgccgc
     2461 cgaccgtccg gaaatccgcc gggtcatcga cgcccttcgg ggtcgcggcg cctcggggca
     2521 caacgtcacc ccggacgtca tggaattcgt ctcggacctg gaaaccccgc cgggcctggt
     2581 ggttcgggcc gaccgcccgg tttcccccgc cctgcctttg gcggacaacg cgctttgggt
     2641 ggtgctggac ggcctgcaat ccccggccaa cgccggggcc gtggtgcggg tggccgaggc
     2701 cgccggggcc caggccgtca tcgccgccgc gggcaccgcg gacctctgga gccccaaggc
     2761 cctccgcggt tcctccggga gcgcttttcg cgttcccttg gttcatatcc ccgacctgaa
     2821 ggaagtgtcg aaccggctta aagggcgcgg catcgcggcc gtggcggcgg atcaaaaggc
     2881 ggacctggat tattgggatt atgattggac ccgccccacg gcgatccttc tgggagccga
     2941 gggccgcggg ttgggcgatt tgtcggattt cgacgcgcaa cgggttcgcg tgcccatggc
     3001 gggcaaggtg gaatcgctga acgtcgcggt ggcggcgggc cttctgctca tggaggcggc
     3061 gcgccaaagg cggtccaaga gttgaaggtg caaaccgggc tggacctctt gtccaagcgc
     3121 ggccacccgg cgttgaaggg caagcgggtg ggcttgatcg tccaccaagc ctcggtggac
     3181 ggccaactgc gtcacgccgt tgacttatta aggaaccgca aggatttccg cgtggccgcc
     3241 ctcttcgccc cggagcacgg tttttacggc gacctccagg accaggtgcc cgtggacggc
     3301 gcccgggacc cgcgcaccgg cctccccgtg ttcagcctct acggcccccg ccgccaaccc
     3361 accgccccaa tgctgaaggg cctggacgcc ctggtcttcg accttcagga catcggcgtc
     3421 cgatattaca cgttcatctg gaccatggcc ctggccctgg aagccgcggc caagaccgga
     3481 atcacgttcg tcgtcctgga tcgtcccaat cccctgggcg gggcggtgga aggcaacctt
     3541 cccgatccgg cctatgccag cttcgtgggc ctttaccccc tcccggtccg ccacggcaaa
     3601 accgtcggcg aactggccgt ttatttcaac aagaaatacc aatggggcgc ggaccttcac
     3661 gtggtcaaaa tgaagggttg gaaacgatcc atgcggttcg atgaaaccgg cctgccctgg
     3721 gtcatgccct cccccaacat gcccacgctg gacacggcga cggtctacgc cggcatgtgc
     3781 ctgctcgagg ccaccaacct ctccgaaggc cggggcacga cccggccctt cgaaatcgcc
     3841 ggcgccccct tcatcgacgg ccaccgcctg gcccgggcct tggaaaagaa aaatctcccc
     3901 ggggtcgcct tccgccccct ggccttccgc cccacgttta ataaatgggc caaccaaacc
     3961 tgcggcggcg tccaacttca cgtcacgaat ttcaaaacct tcaagtcctt cctgaccggc
     4021 ctgatcttcc tacaaacagt tcaggagctt cacccccggc gcttcaagtg gaatccgccg
     4081 ccgtatgaat acgagacggt caaaaggccc atcgatatcc tctgcggaac cgaccggatt
     4141 cgaaaatcca tcgacgccgg cgacaatctt tttaatctct ccaaaacctg gccc
//
LOCUS       DAOQLS010000099         4179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10438_length_4179_cov_6.986663, whole genome shotgun sequence.
ACCESSION   DAOQLS010000099 DAOQLS010000000
VERSION     DAOQLS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_4179_cov_6.986663"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="rplB"
                     /locus_tag="PK362_02750"
     CDS             complement(<1..785)
                     /gene="rplB"
                     /locus_tag="PK362_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNC73792.1"
                     /translation="MPMKSFKAYTPSRRFITVEDFSEITKTTPEKALLRPLTKKGGRN
                     NTGQVMVRFRGGGHKRMYRVIDFKREKFGVPAKVAAIEYDPNRSARLALLHYRDGEKR
                     YILQPVGIKPGDVLMSGPTAEIKNGNALPLANIPVGTIVHAVEMVPGNGAQFARSAGA
                     SVQIMAREGGYVTIKMPSGEIRLVPDGGMATIGQVGNTEHENLTIGNAGRNRHRGFKS
                     HVRGTAMNAVDHPHGGGRGRSKGNNQPRSPWNQPAKGYKTRSD"
     gene            complement(804..1091)
                     /locus_tag="PK362_02755"
     CDS             complement(804..1091)
                     /locus_tag="PK362_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNC73793.1"
                     /translation="MPLADVLRRPLVTERTTQLREQNKYVFEVDPGATKGQIREAVQS
                     AFKVDVLDVNTMNVPGKLRRRGAHAGYQSSWKKAIVKLKAGQEIKYAEPAK"
     gene            complement(1096..1740)
                     /gene="rplD"
                     /locus_tag="PK362_02760"
     CDS             complement(1096..1740)
                     /gene="rplD"
                     /locus_tag="PK362_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNC73794.1"
                     /translation="MSLTVDVINNKGSKVGSADLSERVFGVKAPRALIHEVVTAYLAN
                     VRRGTHSTKTRGEVSGGGLKPWKQKHTGRARSGSIRSPLWRHGGIIFGPKPRSYYQAV
                     PPAKRRLALKAVLSDFATAGKLRVIEDFDVKEAKTKAAAELARGLKLAPKSVVVVTAL
                     SPTLLRAARNLADLRVCRAQDLNSYNAMLADELVFTRAALEQITQRLVGAAEAN"
     gene            complement(1750..2517)
                     /gene="rplC"
                     /locus_tag="PK362_02765"
     CDS             complement(1750..2517)
                     /gene="rplC"
                     /locus_tag="PK362_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNC73795.1"
                     /translation="MTEQATEPKATDATAQPGKAPALKAILAKKIGMTRVYNPKGEFI
                     PVTVLESAACRVVRVVTQAKNGYEAVQLGLGDVKAKNINKPESGHFAAANVPMSRWLR
                     EFRVPKTDGFTAGQTVHVDQFAAGDAVDVSGIIKGKGFAGAVKRHRFKGGPKTHGQSD
                     RWRAPGSSGRERVWPGKRGPGHMGSEWKTVQRLEVVSVDPDQNLLLLRGSVPGPDGTC
                     VSVYQTTRPRRAKKAVAAAAAKKAAKAPAKAAKPEKK"
     gene            complement(2527..2853)
                     /gene="rpsJ"
                     /locus_tag="PK362_02770"
     CDS             complement(2527..2853)
                     /gene="rpsJ"
                     /locus_tag="PK362_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNC73796.1"
                     /translation="MSAPAPAAQQKIRIRLRAYDHRMLDDSLAKIVETAQRTGAALAG
                     PVLLPTQIKKYTVNRSVHTDKKSREQFEMRIHKRLVDILEPTAKTVEEMMKLDLPAGV
                     DVEIKL"
     gene            complement(2980..4176)
                     /gene="tuf"
                     /locus_tag="PK362_02775"
     CDS             complement(2980..4176)
                     /gene="tuf"
                     /locus_tag="PK362_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNC73797.1"
                     /translation="MAKPKFERTKPHVNVGTIGHVDHGKTTLTASILQVLSKQGKAQI
                     KSYQDIAKGGTVRDDSKIVTIAVSHVEYESEKRHYAHIDCPGHADYIKNMITGAAQMD
                     GAILVVSAADGPMPQTREHVLLARQVNVPNLVVFLNKVDLVDDPELLDLVEIEVRDLL
                     KKYEFPGDKVPVIRGSSMKAMQGDQSEIGEKSIMKLMEALDSAIPDPKRELDKPCLMS
                     VEDVFTITGRGTVATGRIERGRMKVGDNVEIVGIQDTKKSVVTGIEMFRKVMDEAQAG
                     DNVGVLLRGIEKDQIERGQVICAPGSIQPHKKFKGQVYILTKEEGGRHTPFFNGYRPQ
                     FYVRTTDVTGSLKLKAGVEMVMPGDNVEVEVELMVPVALEKNVRFAIREGGHTVGAGV
                     VTEILA"
BASE COUNT          604 a         1403 c         1268 g          904 t
ORIGIN      
        1 ttgtccgacc gggtcttgta acccttggcc ggttggttcc acggcgaccg gggctggttg
       61 ttccccttgg accggccgcg gccgccgccg tgcgggtggt ccaccgcgtt catggccgtg
      121 ccgcgaacgt gggacttgaa cccgcgatgg cgattgcgcc ccgcgttgcc gatggtcaaa
      181 ttttcgtgct cggtgttgcc cacctggccg atggtggcca tgccgccgtc cggcaccagg
      241 cggatttcgc ccgagggcat cttgatcgtc acgtagccgc cttcgcgggc catgatctgg
      301 accgaggcgc cggccgagcg cgcgaactgg gcgccgttgc cgggcaccat ttccaccgcg
      361 tgcacgatcg tgccgacggg gatgttggcc aggggcaggg cgttgccgtt cttgatttcg
      421 gccgtcgggc cggacatcag gacgtcgccc ggcttgatgc cgacgggctg caggatgtag
      481 cgcttctcgc cgtcccggta gtggagcagg gccaggcggg cggagcggtt cgggtcgtac
      541 tcgatggccg cgactttggc cggcacgccg aacttttcgc gcttgaaatc gatgacgcgg
      601 tacatccgtt tgtgcccgcc gccgcggaag cggaccatga cttgaccggt gttgttgcgg
      661 ccgccttttt tggtgagcgg acgcaggagg gctttctcgg gcgtggtctt ggtgatctcc
      721 gaaaagtcct cgacggtgat gaaccgtcgc gacggggtgt aggctttaaa ggatttcatc
      781 ggcatggcgt tttctttccc cggttatttc gcgggttccg cgtatttgat ttcttggccg
      841 gccttgagct tcacgatggc ctttttccaa gaggattggt agccggcgtg ggcgccgcga
      901 cggcggagct tgcccggcac gttcatggtg ttcacgtcga gcacgtccac cttgaaggcg
      961 gactgcacgg cttcccgaat ttggcccttg gtggcgcccg ggtcgacttc aaaaacgtat
     1021 ttgttctgtt cgcgcaactg cgtcgtgcgc tcggtgacga gcggccgacg aagaacgtcc
     1081 gccaggggca tcatgtcagt tcgcctccgc cgcgccgacg aggcgctggg tgatttgttc
     1141 gagggccgcg cgggtgaaca ccaactcgtc ggccagcatc gcgttgtagc tgttgaggtc
     1201 ctgggcgcgg cagaccctca aatccgccag gttgcgcgcg gcgcggagca gggtcgggct
     1261 gagggccgtc accaccacga cggacttggg cgccaatttc agaccgcgcg ccaattccgc
     1321 ggcggctttc gtcttggcct ctttcacgtc gaaatcttcg atgacccgga gcttgccggc
     1381 ggtggcgaaa tccgacaaca cggccttgag cgccagtcgg cgcttggccg ggggaaccgc
     1441 ctggtaatag ctgcgcggct tgggaccgaa aatgatgccg ccgtggcgcc acaggggcga
     1501 ccgaatggac cccgaccggg cgcggccggt gtgtttttgc ttccagggct tcaaaccgcc
     1561 gccggacact tcgccgcggg ttttggtgga atgcgttccg cgccgcacgt tggccaggta
     1621 ggcggtgacc acttcgtgga tcagggcgcg cggggccttc acgccgaaca cgcgctcgga
     1681 caggtcggcg ctgccgacct tggacccttt gttgttgatg acgtcaacgg tcaaagacat
     1741 aaattcctct tatttcttct cgggcttcgc ggccttggcc ggggccttgg cggctttttt
     1801 cgccgcggcg gccgcgacgg ctttcttggc gcggcggggc cgcgtggttt gatacaccga
     1861 cacgcaggtt ccgtccggac cgggcacgga gccgcggagc agcagcaaat tctgatcggg
     1921 gtccacgctc acgacttcga ggcgctggac ggttttccac tcggagccca tgtggccggg
     1981 gccgcgtttg ccgggccaca cgcgttcgcg gcccgaggag ccgggcgcgc gccatcggtc
     2041 cgattgaccg tgggtcttgg gaccgccttt gaaacggtgg cgcttcacgg cgccggcgaa
     2101 ccccttgcct ttgatgatgc cggacacgtc cacggcgtcg cccgcggcga actggtccac
     2161 gtgcacggtc tgcccggcgg tgaacccgtc ggtcttgggc acgcggaatt cccgcagcca
     2221 gcgcgacatc ggcacgttgg ccgcggcgaa gtggccggat tccggcttgt tgatgttctt
     2281 cgccttcacg tcgcccaacc ccagctggac ggcctcgtag ccgtttttcg cctgggtcac
     2341 gacgcgcacc acgcggcagg cggccgattc caacacggtg acgggaatga attccccttt
     2401 ggggttgtac acccgggtca tcccgatttt cttcgccagg atggctttca gcgccggggc
     2461 tttcccgggc tgggccgtgg cgtctgtcgc ttttggttcg gtcgcttgtt ccgtcatggg
     2521 ttcaacctaa agtttaatct ccacgtccac acccgccggc aaatcgagtt tcatcatttc
     2581 ctcgacggtt ttggccgtgg gctcgagaat gtccaccaac cgcttatgaa tgcgcatttc
     2641 aaattgttcg cgcgatttct tgtccgtgtg caccgatcgg ttcacggtgt acttcttgat
     2701 ctgcgtcggc aggagcaccg ggccggccag ggccgcgccg gtgcgttggg ccgtttcgac
     2761 gatcttcgcg agcgagtcgt ccaacatccg gtggtcatag gcccgaaggc ggatgcgaat
     2821 cttctgctgg gcggcggggg cgggagcgct catgtcagtt caccgagaag acgcagtcca
     2881 cggacccgtc ggcccgggcg tccaccacgc ggagcgtcac ttcgtgacgt ccgtcgaaca
     2941 tttccacttc caccgtcgct gtcgtcatag ccctcgtcct tacgccaaaa tttcggtgac
     3001 gacgccggcg cccaccgtgt ggccgccttc gcggatcgcg aaccgcacgt tcttctccaa
     3061 cgccaccggc accatcaact ccacttccac ttccacgttg tctccgggca tcaccatctc
     3121 caccccggcc ttcaacttca aactccccgt cacgtccgtc gtccgcacgt agaactgcgg
     3181 ccggtatccg ttgaagaacg gcgtgtgtcg cccgccttct tccttcgtca aaatatacac
     3241 ctgccccttg aacttcttgt gcggttggat cgaccccggc gcgcaaatca cctgcccgcg
     3301 ctcgatctgg tccttttcga tcccacgcag cagcacgccc acgttgtccc cggcctgcgc
     3361 ctcgtccatc accttccgga acatttcgat ccccgtcacg accgacttct tcgtgtcctg
     3421 gatccccacg atctccacgt tgtcgcccac cttcatgcga ccccgctcga tccgtcccgt
     3481 cgccaccgtc ccccgtcccg tgatcgtgaa cacgtcttcc accgacatca aacacggctt
     3541 gtccagttcc cgcttcgggt ccggaatcgc gctgtccagg gcttccatca gcttcatgat
     3601 cgacttctcg ccgatctcgc tctggtcccc ctgcatcgcc ttcatgctgc ttccgcggat
     3661 caccggcacc ttgtccccgg ggaactcgta cttcttcaac aaatcccgca cttcgatctc
     3721 caccaggtcc aacagctccg ggtcgtccac caaatccact ttgttcaaga acaccaccag
     3781 gttcggcacg ttcacctgcc gcgccaacag cacgtgttcc cgcgtctgcg gcatcggccc
     3841 gtccgccgcc gacaccacca agatcgcccc gtccatctgc gccgcgcccg tgatcatgtt
     3901 cttgatgtag tccgcgtggc ccgggcagtc gatgtgcgcg tagtgccgct tctccgactc
     3961 gtattccacg tgcgacaccg cgatcgtcac gatcttcgaa tcgtcccgca ccgtcccgcc
     4021 cttcgcaatg tcctggtacg acttgatctg cgctttcccc tgcttcgaca acacctgcag
     4081 gatcgacgcc gtcaacgtcg ttttcccgtg gtccacgtgg ccgatcgtcc ccacgttcac
     4141 gtgcggtttc gtgcgctcaa atttgggttt tgccatggt
//
LOCUS       DAOQLS010000100         4177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10453_length_4177_cov_4.579816, whole genome shotgun sequence.
ACCESSION   DAOQLS010000100 DAOQLS010000000
VERSION     DAOQLS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10453_length_4177_cov_4.579816"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /gene="rlmB"
                     /locus_tag="PK362_02780"
     CDS             complement(<1..675)
                     /gene="rlmB"
                     /locus_tag="PK362_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNC73798.1"
                     /translation="MENELTSDLVWGRHPVAAALTAGRVNKLWILRGASGLEDVLNAA
                     RASRVVFQWVDRQRLDGLTGGAVHQGVAARVSAHEYQTLDDLLSAGRPSPLLLLDGVT
                     DPHNLGAILRNAAFFGARGVVIPRWRSAGMTGAVDKASAGAAGLVPLVQVANIGQTIL
                     ELKKKDYWVYGADAAGRSCAAFDFAQPVALVIGAEGTGLHRLVRERCDDLIAVPGEAK
                     VPSLNAS"
     gene            complement(660..2084)
                     /gene="cysS"
                     /locus_tag="PK362_02785"
     CDS             complement(660..2084)
                     /gene="cysS"
                     /locus_tag="PK362_02785"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNC73799.1"
                     /translation="MTPRPLRFHSTLTGREENFEPGTPGRVSLYVCGVTPYDECHLGH
                     ARCYVTFDFIRRCLERLGYAVTHVQNFTDVDDKIIRRAAERGEAPEVLADRYIADYFD
                     KMDRLGVQRAAIYPRVTAHVPAIVSFIERLVAKGLAYPRDGDVYYSVRKFPAYGRLAK
                     RSPDDLLSGARVEVDVRKDDPLDFALWKAAKPGEPAWPSPWGPGRPGWHIECSVMSLA
                     NLKTDTVDIHGGGQDLIFPHHENEIAQSEGATGKPFARYWIHNGFVTVNKEKMSKSLG
                     NFFTLTDIFKLHAPRVVRFFLLSHHYRGPLEFSDDLLKQAADRLAEIDEDFRRLEAGL
                     KTPLADKPRNAKALEKILTEFPDRLDEALADNFNSPRALAVVHALLGELKTRLETPKG
                     LSTDALRRGVALLREAFEGVLGIEGAAASEGADGDIQSLVDQRTAARKGKNWAEADRL
                     RAELARRGVVVEDTPQGPRWWKTN"
     gene            complement(2081..2593)
                     /gene="ispF"
                     /locus_tag="PK362_02790"
     CDS             complement(2081..2593)
                     /gene="ispF"
                     /locus_tag="PK362_02790"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNC73800.1"
                     /translation="MSAVVSPRTPVPRVGFGYDSHRFKKGRPLILGGVRIPHDRGLEG
                     HSDADVLTHAVIDALIGAAGRGDIGTWYSDRDPRFKNADSLKLLADARRRTARVHEVV
                     NVDATLLAETPRLGPYKNRIRARLARALGVPPSRVNVKAKTNEGMGFVGRREGLAALA
                     VVALAPRVRS"
     gene            complement(2590..3282)
                     /gene="ispD"
                     /locus_tag="PK362_02795"
     CDS             complement(2590..3282)
                     /gene="ispD"
                     /locus_tag="PK362_02795"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNC73801.1"
                     /translation="MPVGAVLVAAGRGRRFGAGGPPKQFRSLNGRPLFHWPLRVLERT
                     PAVTAIVMVVPQDRRLWTDRYLKRAGFKKVVAVVPGGAERFDSVRAGLRALPGPCDLV
                     LVHDAARALVTRPVVDRVIRAARRTGAALAAWPVPDTIKEGVQRAGRWSVRRTVPRTG
                     LWLAQTPQGFRRTETRAFFEMTGPLTDDVQALERAGRPVEIVRGSADNFKVTLPEDFD
                     LCRRLLTARKDR"
     gene            complement(3289..>4177)
                     /locus_tag="PK362_02800"
     CDS             complement(3289..>4177)
                     /locus_tag="PK362_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016089677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNC73802.1"
                     /translation="DALISAVIGAVLGLVTAQLLNWVTFQLGSDKVFAFAQKGSLISH
                     LVLSYLGMVLFVRKRSELELLDRDLIVKGAKKKALATHLVDTSVLIDGRIADICETKF
                     IYGTLVVPRFILQELQAVADSSDGAKRARGRRGMDILARLQENPEVPVRIFDKDYPDI
                     KEADSKLVALAKDLGAKVLTTDFNLNKIASLQGVTVLNVNDLANALKPVVLPGEIMNV
                     FVLKEGKERDQGVAYLDDGTMVVVEEGRRAIGQKVHATVTSILQTSAGRMIFTKMSRG
                     HAEPAAAAERPATPAHPDL"
BASE COUNT          745 a         1373 c         1358 g          701 t
ORIGIN      
        1 ggaagcgttt aaagaaggaa ctttggcttc ccccggcacc gcgatcagat cgtcgcaacg
       61 ctcgcggacc aaccggtgca accccgttcc ctcggccccg atgaccaagg cgaccggctg
      121 ggcgaaatcg aaggcggcgc aggagcgccc ggcggcgtcg gcgccgtaga cccagtaatc
      181 ctttttcttg agctccagaa tggtttgacc gatgttggcc acttgaacga ggggcaccaa
      241 gccggcggcg cccgccgacg ccttgtccac ggcgccggtc atgccggccg accgccaacg
      301 gggaatcacg acgccccggg cgccgaagaa cgccgcgttt cgaaggatcg cgcccaggtt
      361 gtggggatcg gtcacgccgt cgagcaacag caggggcgac ggtcttcccg ccgaaagcaa
      421 atcgtccagg gtttggtatt cgtgggcgga gacccgcgcg gccacgccct ggtgcacggc
      481 cccgccggtc aggccgtcca gacgttgccg gtccacccac tgaaaaacga cacgggaggc
      541 ccgggcggcg ttcaacacgt cttccaagcc cgaagcgccg cgaaggatcc acagcttgtt
      601 cacccggccg gccgtgagcg cggcggccac ggggtgccgc ccccaaacca aatccgacgt
      661 cagttcgttt tccaccaacg aggcccctgg ggcgtgtctt cgacgacgac gccccggcgc
      721 gcgagttcgg cgcgcaaacg atcggcttcc gcccaatttt tccctttgcg ggcggcggtc
      781 cgctgatcca ccagggattg gatgtccccg tcggcccctt ccgacgccgc cgccccctcg
      841 atgcccaaca ccccttcgaa cgcctcccgc agcaaggcga cgccccggcg aagggcatcg
      901 gtggaaagcc ctttgggggt ctccagccgc gttttcaact cgcccagcag agcgtgcacc
      961 acggccaggg cccggggcga attgaaattg tccgccaggg cttcgtccaa gcgatcggga
     1021 aattcggtca ggattttctc caacgccttc gcgttccttg gtttgtcggc cagcggggtt
     1081 ttaagtcccg cctccaaccg acgaaaatcc tcgtcaattt cggccagtcg atccgcggcc
     1141 tgcttgagca aatcgtcgga aaattccagg gggccccggt agtggtggct cagcaggaaa
     1201 aaccggacga cccggggcgc gtgcaattta aagatgtccg tcagggtgaa aaaatttccc
     1261 agggatttgg acattttctc cttgttgacg gtcacgaacc cgttgtggat ccaataccgg
     1321 gcgaacggct tgcccgtggc tccctcggac tgggcgatct cgttttcgtg gtgggggaaa
     1381 atcaaatcct gcccgccccc gtggatatcg acggtgtcgg tctttagatt cgcgaggctc
     1441 atgacggaac attcgatgtg ccaaccgggg cgtccgggtc cccagggaga cggccaggcg
     1501 ggttcccccg gtttggccgc tttccacagg gcgaaatcca ggggatcgtc cttgcggacg
     1561 tccacctcga cccgggcgcc ggacaaaaga tcgtcggggg accgcttggc caaccgcccg
     1621 taagcgggaa attttcgcac ggaatagtag acgtcgccgt cgcggggata ggccagtccc
     1681 ttggcgacga gtcgctcaat gaaggaaacg atggccggga cgtgggcggt cacccggggg
     1741 tagatggccg cccgttgcac cccgagacgg tccatcttgt cgaaataatc ggcgatgtac
     1801 cggtccgcca ggacctcggg ggcttcgccg cgttccgccg cgcggcggat gattttgtcg
     1861 tccacatcgg tgaaattctg gacgtgggtc acggcgtatc ccaggcgctc caggcaccgg
     1921 cgtatgaaat cgaaggtgac gtagcagcgg gcgtggccca aatggcattc gtcgtagggg
     1981 gtgacgccgc agacgtacaa ggaaacccgc ccgggcgtgc cgggctcgaa attttcttcg
     2041 cggccggtga gcgtgctgtg gaagcggagc gggcgggggg tcatgaacga acgcgcgggg
     2101 ccaaagcgac gacggccagg gcggccagcc cctcccgtcg accgacgaat cccatgcctt
     2161 cgttggtctt ggccttgacg ttgacgcggg acgggggaac gcccagggcc cgggccagtc
     2221 gcgcccgaat ccggtttttg taggggccca ggcgcggggt ttcggccaac agcgtggcgt
     2281 ccacgttgac cacctcgtgg acccgggccg tgcgccggcg ggcgtcggcg agcaatttca
     2341 aactgtcggc gttcttgaaa cgcggatccc ggtcggaata ccacgtcccg atgtcgcccc
     2401 ggccggcggc gccgatcaac gcgtcgatca ccgcgtgggt cagcacgtcc gcgtcggaat
     2461 ggccctccag cccccggtcg tggggaatcc gcaccccgcc caggatcaac ggccggcctt
     2521 ttttgaaccg atggctgtcg taaccgaacc ccacgcgggg gacgggcgtc cgcggcgaaa
     2581 ccacggcgct caccggtcct ttcgggcggt caaaagccgg cggcacaggt cgaagtcttc
     2641 ggggagcgtc actttgaaat tatcggcgga cccccggacg atttccaccg ggcgtcccgc
     2701 ccgctccaag gcctggacat cgtcggtcaa gggaccggtc atctcgaaaa aagcgcgggt
     2761 ttcggtgcgt cgaaatccct ggggggtttg ggccaaccag agtcccgtac gggggaccgt
     2821 ccggcggacc gaccaacgtc cggcgcgttg aacgccttct ttgatcgtgt cgggcacggg
     2881 ccaggcggcc agggccgcgc cggtgcggcg cgcggcgcgg atcacccgat cgacgacggg
     2941 gcgcgtgacc aaggcccggg cggcgtcgtg caccaggacg agatcgcagg ggcccgggag
     3001 ggcccgcaga ccggcccgaa ccgaatcgaa ccgttcggcc ccgccgggaa ccaccgccac
     3061 cactttttta aacccggcgc gtttcaggta acgatccgtc cacaagcggc gatcctgggg
     3121 gaccaccatc acgatggcgg tcacggcggg cgtccgttcc aggacgcgga gcggccaatg
     3181 gaacaggggc cgcccgttta gggagcggaa ttgcttgggc ggaccgccgg cgccgaaacg
     3241 gcgaccgcgg cccgcggcca cgaggaccgc gccgaccggc atgggccctt acaggtccgg
     3301 gtgggccggc gtcgccggcc gttccgcggc ggcggccggt tcggcgtgcc cgcgggacat
     3361 cttggtgaaa atcatccgcc cggcggaggt ttgcagaatc gacgtcaccg tggcgtggac
     3421 tttttgcccg atggcccggc ggccttcctc gaccaccacc atcgtcccgt cgtccagata
     3481 ggcgacgccc tggtcccgct ccttgccttc tttgagcaca aaaacgttca tgatttcgcc
     3541 ggggagcacc acgggcttca aggcgttggc cagatcgttg acgttcaaga ccgtgacgcc
     3601 ctggaggctc gcgattttgt tgaggttgaa gtccgtcgtc aacaccttgg cccccagatc
     3661 cttggccagg gcgaccaact tggaatcggc ttccttgatg tcggggtaat ctttgtcaaa
     3721 aatgcggacc ggcacctccg ggttttcctg aagccgcgcg agaatgtcca tgccgcgccg
     3781 cccccgggcc cgcttggccc cgtcggagga atccgccacg gcctgaagct cctggaggat
     3841 gaaccgggga accaccaacg ttccataaat aaatttcgtc tcgcagatgt cggcgatgcg
     3901 gccgtcgata aggacggacg tgtccaccaa gtgggtggcc aacgcttttt tcttcgcccc
     3961 tttgacgatc aggtcccggt ccaacagttc caactccgac cgtttccgga caaagagcac
     4021 catgccgaga tacgacagca ccaaatggga aatcagggac cccttctgcg cgaaggcaaa
     4081 gactttgtcg cttcccaatt gaaacgtgac ccagttgagc aattgcgcgg tcaccaaccc
     4141 caacaccgcg ccgatcaccg ccgagatcaa cgcgtcc
//
LOCUS       DAOQLS010000101         4177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10454_length_4177_cov_4.469190, whole genome shotgun sequence.
ACCESSION   DAOQLS010000101 DAOQLS010000000
VERSION     DAOQLS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10454_length_4177_cov_4.469190"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK362_02805"
     CDS             complement(<1..234)
                     /locus_tag="PK362_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC73803.1"
                     /translation="MNPTIRAFLKKEFIQALRDKRMRIIIFAIPVVQITVFGLALSNE
                     IKNVRLALYAAPTDALAQGLHRRRQRRERTTLLL"
     gene            complement(237..1367)
                     /locus_tag="PK362_02810"
     CDS             complement(237..1367)
                     /locus_tag="PK362_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC73804.1"
                     /translation="MIFRRPWFHAGRARAVAKKEIHHVLRDPFVLAMALGVPVFLVVF
                     FGYVLDFDVKRIALTVVDHDQTRASRELVDLFSSAAYFRPRPPEAGRTPLESLEEGRA
                     KAALFIEPGFGGRLADGVPTRVQIVLDGSDNQTTGVVGSYLGGLQRAAARRASGATPG
                     RNVELRTRFLFNPELNSRWFIVTGLFVVAVGVLSILLTALTVAREWETGSMELLLSTP
                     VRPLEIILGKLAPYMGLCLGAVALVYAAARLAFGVPFRGNHLVFLLACVLFLACTLAQ
                     GLLISVVTRQQQLAMQLSNITGLLPATLLSGFIFPVESMPLFFRVLTSLLPPRWFIDC
                     CRGIFLKGAGLRELAVPLGALTLIATGLILLATKKFKRDLEP"
     gene            complement(1364..2278)
                     /locus_tag="PK362_02815"
     CDS             complement(1364..2278)
                     /locus_tag="PK362_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73805.1"
                     /translation="MTAAVETENLTVRFGDFTAVDRVTLAVGRGEIFGFLGANGAGKT
                     TTIRVLTGLLPPTDGVARVAGLSFADGPDAIKKRVGYMSQKFTLYNDLNVGENLEFAA
                     GLRKIPRRIFQERARRLLEFVRFDHPLSTLVRDLPGGLKQEVSLAAALLHEPDVVFLD
                     EPTAGVSPAARARFWTLIRRLAAEGRTVFVTTHYMDEAEECGRIALMRAGRIIALGAP
                     AELKRDAFPERLLELEWAGEPPAGWREELLKRAALTVVPHGLYHHALVRDEEAFAAFS
                     RNRPALTVRTVAPSLEDVFIRLVEGRDR"
     gene            complement(2275..3021)
                     /locus_tag="PK362_02820"
     CDS             complement(2275..3021)
                     /locus_tag="PK362_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC73806.1"
                     /translation="MPLALHATDLRKRLGPHAALDGVTVDFTAGGLHGLIGSDGAGKT
                     TLLRTLVGLLRPDAGAVQYRDGARSLSLEEARPGLAYMPQQQSLYGDLSISEHLDFFR
                     DLYGIPPSIYRDRRARLLGLTRLDKFQDRPAGKLSGGMYKKLGLMCALLRTPTALLLD
                     EPTNGVDPISRREFWDLLHDLRREGLLIIVATAYMDEAERCAHVHLMENGRVLAAGAP
                     RDLLSREGVRGFSEYFIRRAEGAGPIGGSR"
     gene            complement(3050..3769)
                     /locus_tag="PK362_02825"
     CDS             complement(3050..3769)
                     /locus_tag="PK362_02825"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC73807.1"
                     /translation="MRKKIVPFLAAALVLGAGIARWGCRRPFRYAGTLEVTEVGLSPR
                     VAGPIREVAVREGDAVKKDQIVVRLDGDDLRLAADLADRDFRRGVTLNKNGSLPDEAL
                     DRLRFKRDDTALRLSWCDLRSPLDGVVLHRHREPGEWVAPAQNLLTLADGAHPYAYFY
                     VAAPVMARLSVGQKVAATLPELGDRPANGVIVYVRGEAEFTPKNVQTRTERERLVFGV
                     KVAFDNADGALKPGMTVETAF"
     gene            complement(3766..>4177)
                     /locus_tag="PK362_02830"
     CDS             complement(3766..>4177)
                     /locus_tag="PK362_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73808.1"
                     /translation="TREDINPSAGANLRWSLFEGGRAVREAEEQASLARAARDRRRQT
                     ENDLRREWAKARDRRAGAREETELNRAAVQERTELARLTYDAYRAGRTPFLEVQSANL
                     QLLDAQTALARSRLAELTQLAVLESLSSKGSVSP"
BASE COUNT          775 a         1341 c         1412 g          649 t
ORIGIN      
        1 cagaagaagc gtggtccgct cgcgccgctg tcgccgacgg tgaaggccct gggccagagc
       61 gtccgtcggc gcggcgtaca gcgccagtcg gacgtttttg atttcgttcg acaacgccag
      121 gccgaaaacc gtgatctgca cgacggggat cgcgaaaata atgatccgca tgcgtttgtc
      181 ccgcaaggct tgaatgaatt cttttttgag gaaggcccgg atcgtcgggt tcaaggtcag
      241 ggttccagat cccgtttaaa ttttttcgtc gccaggagaa tgaggccggt cgcgatcagg
      301 gtcaacgcgc cgagcgggac cgccaattcc cgaagcccgg cgcccttgag gaaaatgccc
      361 cggcagcaat cgatgaacca acggggcggc aacagggagg tgaggacgcg gaaaaagagg
      421 ggcatgctct ccacggggaa aatgaacccc gagaggagcg tggcgggcag aagccccgtg
      481 atgttggaga gctgcatggc caattgttgc tggcgggtca cgacggaaat caacagaccc
      541 tgggccaggg tgcaggccaa aaacaaaacg caggccaaga ggaaaacaag gtggttcccc
      601 cgaaagggga ccccgaaggc cagccgcgcc gccgcgtaga cgagcgccac cgcgcccagg
      661 caaaggccca tgtagggagc caactttccc aaaataattt ccaggggccg caccggcgtg
      721 gagagcagca gttccatgga ccccgtctcc cattcccggg cgacggtgag ggccgtgagc
      781 aaaatcgaaa ggacgcccac ggccacgacg aacaacccgg tgacgatgaa ccaccggctg
      841 ttgagctcgg gattgaaaag gaaccgggtg cgaagctcca cgttccggcc gggcgtcgcc
      901 ccggacgcgc gacgggcggc ggcgcgctgg aggccgccca agtaacttcc cacgaccccg
      961 gtggtttggt tgtcggaacc gtccaaaacg atttgaaccc tggtcggaac gccgtcggcc
     1021 aaacggccgc cgaaccctgg ttcgatgaaa agggcggctt tggcccggcc ctcctccagg
     1081 ctttccaggg gagtccggcc ggcttccggc ggccgggggc gaaaataggc cgcgctcgaa
     1141 aagaggtcca cgagctcccg ggaggcccgg gtttggtcgt ggtccaccac ggtcaaggcg
     1201 attcgtttca catcaaagtc cagcacgtag ccgaaaaaga cgacgagaaa cacgggcacg
     1261 cccaaggcca tggccaaaac gaagggatcc cgaaggacgt gatggatttc ctttttggcc
     1321 acggcccggg cccggcccgc gtgaaaccac ggccgccgga aaatcatcgg tcgcgtcctt
     1381 ccacgaggcg gatgaacacg tcttccaagg aaggcgccac cgtccggacc gtcaaggccg
     1441 ggcggtttcg ggagaacgcc gcgaaagcct cttcgtcccg gaccagcgcg tgatgataga
     1501 gtccgtgggg gaccacggtg agcgccgcgc gcttcaacaa ctcctcccgc cagccggcgg
     1561 ggggctcccc ggcccattcc aattccagca gacgttcggg aaaggcgtcc cgtttcaatt
     1621 ccgcgggcgc cccgagggcg atgatccgcc ccgcgcgcat gagcgcgatg cggccgcatt
     1681 cctcggcctc gtccatgtag tgggtggtca cgaagaccgt gcgcccctcg gcggccaacc
     1741 gccgaatgag ggtccaaaat cgggcccggg ccgcggggga aaccccggcg gtgggttcgt
     1801 ccagaaagac gacgtcgggt tcgtgcagca gggcggccgc gagggacacc tcttgtttca
     1861 aaccccccgg gagatcccgc acgagcgtcg agagcggatg gtcgaaccgg acgaattcca
     1921 acaggcggcg ggcccgttcc tggaaaatcc gccggggtat tttccgaagg ccggccgcga
     1981 attccaaatt ttcgccgacg ttgagatcgt tgtacagggt gaatttttgc gacatgtagc
     2041 cgacgcgttt tttgatggcg tcggggccgt cggcgaagga aaggcccgcc acccgggcga
     2101 cgccatcggt cggaggcagg aggcccgtca agacccggat ggtggtcgtt ttgccggccc
     2161 cgttggcgcc caaaaatcca aaaatctccc cgcgcccgac cgccagggtc acgcggtcca
     2221 cggcggtgaa atccccgaat cgaaccgtca ggttttcggt ttcgacggcg gcggtcaccg
     2281 ggatcccccg atcggcccgg cgccctcggc gcgccggatg aaatactccg aaaacccgcg
     2341 gacgccctcc cgggacagaa gatcccgcgg cgcgcccgcc gccaggaccc ggccgttttc
     2401 catcaaatgc acgtgggcgc atcgctccgc ttcgtccatg taggccgtcg ccacgatgat
     2461 caacagcccc tcccggcgca gatcgtgcag caggtcccaa aactcccggc ggctgatggg
     2521 gtccacgccg ttggtcggct cgtccagcag gagggcggtg ggcgtgcgca aaagggcgca
     2581 catgaggccc aatttcttgt acatcccgcc cgagagcttg ccggccggcc ggtcctggaa
     2641 tttgtccaag cgcgtcagtc cgaggaggcg cgcccggcgg tcgcgataaa tcgacggggg
     2701 aattccgtaa agatcccgaa aaaaatccag atgttcggag atggaaaggt ctccgtagag
     2761 gctctgctgt tggggcatgt aggccaggcc gggccgggcc tcctccaggg aaagggagcg
     2821 ggcgccgtcc cggtattgaa ccgcgcccgc atcgggacgc aggaggccga cgagggtgcg
     2881 aagcagggtc gtcttccccg cgccgtcgga accgatcaag ccgtgaagac ccccggccgt
     2941 gaaatcgacg gtgacgccgt ccagggccgc gtgggggccc aggcgtttgc ggaggtcggt
     3001 ggcgtgaagg gcgaggggca tggggggcgc gggggcccgc gccgtcgcgt tagaacgccg
     3061 tttcgaccgt catgccgggc ttgagggcgc cgtcggcgtt gtcgaaggcg actttcacgc
     3121 cgaagaccag gcgttcccgt tcggtgcggg tttgaacgtt tttcggcgtg aattcggctt
     3181 cgcctcggac gtaaacaatg accccgttcg cgggccggtc gcccaactcc ggaagcgtgg
     3241 cggcgacttt ctgccccacc gacaaccgcg ccatgaccgg cgcggccacg taaaaatagg
     3301 cgtaggggtg cgccccgtcg gcgagggtga gcaggttttg ggcgggggcc acccactccc
     3361 ccggttcccg gtgtcgatgg aggacgaccc cgtccagggg cgatcggaga tcgcaccagg
     3421 aaaggcggag ggccgtgtcg tcgcgcttaa accgcaggcg gtccagggct tcgtcgggca
     3481 gggacccgtt tttattcaaa gtcacgcccc ggcggaaatc ccggtccgcc aaatcggcgg
     3541 ccaatcggag gtcgtccccg tccaatcgga cgacgatttg atcctttttc accgcgtcgc
     3601 cctcccggac ggcgacttcg cggatcggcc ccgccacccg gggcgacaac cccacctccg
     3661 tgacttccag cgtgccggcg tagcggaacg gccggcggca tccccaacgc gcgattcccg
     3721 cgccgagcac cagggccgcc gccagaaacg gaactatttt ttttctcatg gggacaccga
     3781 tcctttcgag gagagggatt ccaagaccgc caattgcgtc aattccgcga gccgggagcg
     3841 ggccagagcg gtttgggcgt cgagcagttg aaggttggcg ctttgcactt ccagaaaggg
     3901 ggtccggccc gcccggtaag cgtcgtacgt cagccgggcc agctcggtcc gttcctgaac
     3961 ggcggcgcgg tttaattcgg tctcctcccg ggccccggcc cggcggtccc gggccttggc
     4021 ccattcccgc cgaagatcgt tctccgtttg tcggcggcgg tcccgggcgg cccgggccag
     4081 ggaggcctgc tcctcggcct cccgaaccgc tcggccccct tcaaacaggg accaccggag
     4141 gttggcgccg gcgctcgggt tgatgtcctc gcgggtg
//
LOCUS       DAOQLS010000102         4160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10518_length_4160_cov_4.452619, whole genome shotgun sequence.
ACCESSION   DAOQLS010000102 DAOQLS010000000
VERSION     DAOQLS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10518_length_4160_cov_4.452619"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PK362_02835"
     CDS             complement(<1..647)
                     /locus_tag="PK362_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC73809.1"
                     /translation="MNIRVLVADAISEEGLVPLQQNPGFSVQVKTGLKGADLLREVSG
                     CDALLVRSETKVNAEVIGAAAGLRFIGRAGTGVDNIDLGAASRQGIVVANVPGGNTIS
                     AAEQTLALLFAMARNIPRADASTRAGKWERSKFVGTEITGKTLGVVGLGRIGREVATR
                     AIGLSMRVIAFDPMGDESWCRRAGIAFVSLDELLAQSDFITMHVPLSDQTRGLLNR"
     gene            complement(689..1825)
                     /locus_tag="PK362_02840"
     CDS             complement(689..1825)
                     /locus_tag="PK362_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNC73810.1"
                     /translation="MKKYYLITPGPTPVPPEVAAQSALPVLHHRTNEFGALFEKAILG
                     MKYVYQTAHDVLMITGSGTGAMESAVANLTSPGDKVIVLTSGSFGERWVKINEAFGTK
                     VVAVRQEWGKAIDPQKAQEALRANPDAKAVFMQHTETSTGVVNDLKTLGQMISETGAI
                     SVVDAISGLGGEELRMDDWKLDVVVTGSQKGLMVAPGLAFAAVGPRAWPVVEKSRNPK
                     FYFDYKQIKKSVGDKETPWTPAVTLVASLVTALEMIKKDGIENVWARHRRLATMARAG
                     MQALGLELLADRPCAVLTSAKLPAAVDGKKLINWLRDEHGVSVAGGQEQLAGKIVRLA
                     HMGYMDHFDLIIGLTALEWALKKFGHTVTPGKAVQVAQEALALA"
     gene            complement(1850..3109)
                     /gene="clpX"
                     /locus_tag="PK362_02845"
     CDS             complement(1850..3109)
                     /gene="clpX"
                     /locus_tag="PK362_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009812289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNC73811.1"
                     /translation="MSTPTGKENKDALQCIFCGKGKREGLRLIGGQGIYVCEDCVRNS
                     HALLTRLTEEEDRRSQRALPKPSQIKKVLDDFVVGQEQAKRILSVAVYNHYRRIEALS
                     KESSPDVELQKSNILFVGPTGTGKTLMAQTLARLLHVPFAIADATTLTEAGYVGEDVE
                     NIILRLLQNANYDVKRAERGIVYIDEIDKTSRKADSPSITRDVSGEGVQQALLKILEG
                     TVAAVPPQGGRKHPQQEYIQVNTTNILFICGGAFEGLEKVIEQRLGKFGMGFGVDPKP
                     KKREDMGDILKHVQPEDLIQYGLIPELVGRLPVLANLENLDEAALVEVLTRPKNALVK
                     QYQKMFSLEGVELSFNEDSLKAIARLAIKRGSGARGLRSILEEILLSTMYELPEQSGL
                     QRVVIDGDVVAKKKDPIRVYESIKKTA"
     gene            complement(3125..3736)
                     /locus_tag="PK362_02850"
     CDS             complement(3125..3736)
                     /locus_tag="PK362_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNC73812.1"
                     /translation="MPGIIPTIIERWNQGAAVGYDIYSRLLKDRIIFIGGYGGAIDTD
                     SANAIIAQLLYLDAEDPERDINLYINSPGGMVTAGLAVYDTIQYIKAPITTICLGMAM
                     SFGALLLAAGRKGKRYALPNARIMIHQPLIYGEGLSGQVTDIAIEAKELDHTKERLTD
                     ILARHTGQPLAKVKADCERNFYMSSDEAKAYGLIDEVFVPRHK"
     gene            complement(3761..>4160)
                     /locus_tag="PK362_02855"
     CDS             complement(3761..>4160)
                     /locus_tag="PK362_02855"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73813.1"
                     /translation="LLGQVILDLGREERARHLTAQVKDYLAQQGASEADWKSAEPKML
                     EKNRPEAERQVRLSYLLSRIAELEKINVEDADVDAQIQKIVDGARPEQRTDVENWMKG
                     RRDTLRAQLREERLFDFLIQQAKVTETPAA"
BASE COUNT          684 a         1238 c         1319 g          919 t
ORIGIN      
        1 cggttcagca ggccgcgggt ttggtccgac aagggcacgt gcatggtgat gaaatccgat
       61 tgggccaaca attcgtccag ggacacgaag gcgatccccg cgcgccggca ccaggactcg
      121 tcgcccatgg gatcgaaagc gatgacgcgc atggagaggc cgatggcccg ggtcgccact
      181 tcgcgcccga tgcggcccaa ccccacgacg cccagggttt tgcccgtgat ctcggttccg
      241 acgaacttgg agcgttccca ttttccggcg cgggtggagg cgtcggcccg ggggatgttt
      301 cgcgccatgg cgaacaaaag cgcgagggtt tgttccgcgg cggagatcgt gttccccccc
      361 ggaacgttcg ccacgacgat gccttggcgg gacgcggccc cgaggtcgat gttgtccacc
      421 ccggtgcccg cccggccgat gaaccggagg ccggcggccg cgccgatgac ctcggcgttc
      481 accttcgttt cggaccggac cagcaaggcg tcgcatccgg acacctcgcg cagcaagtcc
      541 gcgcctttca ggccggtttt gacctgaacg gagaaaccgg gattttgttg aagcgggacg
      601 agcccctctt ccgaaatggc atcggcgaca agaacgcgaa tgttcatggg agtcccttca
      661 cgacggaatc gagatcggtc cttcgcgatt aggcgagggc cagcgcttcc tgggccacct
      721 gcacggcttt gcccggggtg acggtgtggc cgaatttttt caaggcccat tccaaggcgg
      781 tcagcccgat gatcaggtcg aaatggtcca tgtaacccat gtgggccagg cgcacgatct
      841 ttccggccaa ttgttcctgg ccgccggcca cggagacccc gtgttcgtcc cggagccaat
      901 tgatgagctt cttgccgtcc accgcggcgg gaagtttcgc ggaggtgagg acggcgcaag
      961 ggcggtcggc cagaagctcc aaccccaggg cctgcatgcc ggcccgggcc atcgtggcca
     1021 gccgacggtg gcgcgcccac acgttttcga tcccgtcctt tttgatcatc tccaaggcgg
     1081 tcaccaggct cgccaccaac gtcaccgccg gcgtccaagg cgtttccttg tcgcccacgg
     1141 attttttgat ttgcttgtaa tcgaagtaga acttcgggtt ccgggatttc tccacgaccg
     1201 gccacgcgcg ggggccgacg gccgcgaagg ccaagccggg ggccaccatc aggccttttt
     1261 gggagcccgt cacgacgacg tccaatttcc aatcgtccat gcgcagttct tcgccgccca
     1321 gccccgaaat ggcgtccacg accgagatcg ccccggtttc ggaaatcatt tggccgaggg
     1381 tcttcaaatc gttcaccacg ccggtcgagg tttcggtgtg ttgcatgaag accgctttgg
     1441 cgtcggggtt ggcgcgcagg gcttcctggg ctttctgcgg gtcgatggct tttccccatt
     1501 cctgtcgcac cgccacgacc ttggtgccga aggcttcgtt gatcttcacc caacgctcgc
     1561 cgaaggatcc ggaggtcaag acaatcactt tgtcgcccgg ggaggtcagg ttggccaccg
     1621 cggactccat ggcgcccgtg ccggagccgg tgatcatgag aacgtcgtgg gccgtctggt
     1681 aaacgtattt catgcccaaa atggcctttt cgaagagggc cccgaattcg ttggttcggt
     1741 ggtgcaaaac cggcagagcg ctttgggcgg cgacttcggg cggaacgggc gtcgggcccg
     1801 gggtgatgag atagtatttt ttcatggacg atcctccagg tttagaaaat caggcagttt
     1861 tcttaatgct ctcgtaaacg cgaatcgggt ccttcttctt cgccaccacg tccccgtcga
     1921 tcacgacccg ctgaaggccc gactgctcgg gcagctcgta catcgtgctc aacaggattt
     1981 cttccagaat cgagcgcaag ccccgggccc cggagccccg tttgatggcc aggcgcgcga
     2041 tggctttgag gctgtcttcg ttgaaggaga gctccacgcc ttccagggag aacatttttt
     2101 ggtattgctt gacgagcgcg tttttggggc gggtgagcac ctccaccaag gcggcttcgt
     2161 ccagattttc caggttcgcc agcaccggca agcgacccac cagttcggga atcaggccgt
     2221 attgaattaa gtcttcgggt tggacgtgct tcaaaatgtc gcccatgtcc tcccgttttt
     2281 tgggtttggg atcgacgccg aaccccatgc cgaatttgcc gaggcgctgt tcgatgactt
     2341 tttccagccc ctcaaaggcc ccgccgcaaa tgaaaagaat gttggtcgtg ttgacctgga
     2401 tgtattcctg ttgggggtgc ttgcggccgc cctggggcgg caccgcggcc acggtccctt
     2461 ccaggatttt caggagcgcc tgctggaccc cttccccgga cacgtcccgg gtgatggagg
     2521 gcgaatcggc cttgcgggag gttttgtcga tttcgtcgat gtaaacgatg ccccgctcgg
     2581 cccgtttgac gtcgtaattg gcgttttgga ggaggcggag gatgatgttt tccacgtctt
     2641 cccccacgta gccggcttcg gtcagggtcg tggcgtcggc gatggcgaag ggcacgtgca
     2701 atagccgcgc gagggtttga gccatcaagg ttttccccgt gcccgtgggc ccaacaaaga
     2761 gaatgttgga tttttgcagt tccacgtcgg gggacgattc tttcgagagc gcttcgatgc
     2821 gccggtagtg gttgtagacg gccaccgaaa ggatccgttt ggcctgttcc tggcccacga
     2881 cgaagtcgtc cagcactttc ttgatttggg acggtttggg aagcgctcgt tgggaccggc
     2941 ggtcctcctc ttccgtcagg cgggtcagca gggcgtggga atttcgaacg cagtcctcgc
     3001 agacgtagat gccttggccg ccgatgaggc ggaggccctc gcgtttccct ttcccgcaga
     3061 aaatgcactg cagggcgtct ttgttttctt tgccggtggg ggtcgacacg tcgatggcgg
     3121 cgccttattt gtgccggggc acgaacactt cgtcgatcaa tccgtaggct ttcgcctcgt
     3181 cggaagacat gtagaagttg cgctcgcagt cggctttgac tttggccagg ggctgtcccg
     3241 tgtgccgcgc caggatgtcg gtgagccgtt ccttcgtgtg gtccagttcc ttggcttcga
     3301 tggcgatgtc cgtgacctgc cccgagagcc cttccccgta tatcagcggc tggtggatca
     3361 tgatgcgcgc gttgggcagg gcgtagcgtt tccctttgcg tcccgcggcc aggaggagcg
     3421 cgccgaagga catggccatc ccgaggcaaa tcgtcgtgat gggggctttg atgtattgaa
     3481 tcgtgtcgta gaccgccaag cccgccgtca ccatgccgcc cggggagttg atgtacaggt
     3541 tgatgtcccg ttcggggtct tcggcgtcga ggtaaagcag ttgggcgatg atggcgttgg
     3601 ccgaatccgt gtcgatggcc cccccgtacc cgccgatgaa aatgatccgg tctttcagga
     3661 gccgggaata gatgtcgtac cccacggccg cgccttggtt ccagcgttcg atgatggttg
     3721 ggatgatgcc gggcatagcg attccccctt cttgggtgtg ttaagccgcc ggagtttcgg
     3781 tgactttggc ctgttggatc aggaaatcga aaaggcgttc ctcgcgcagt tgggcgcgaa
     3841 gggtgtcgcg acgccctttc atccaatttt ccacgtccgt ccgctgttcc ggccgggcgc
     3901 cgtccacgat tttttgaatt tgggcgtcca cgtccgcgtc ttccacgttg attttttcca
     3961 attccgcgat tcgggacaaa agataggaca gtcgaacctg acgctcggcc tcggggcgat
     4021 ttttttcgag cattttcggt tccgccgatt tccaatcggc ctcggacgcc ccctgctggg
     4081 ccaggtaatc cttgacctgg gccgtgaggt gacgggcccg ttcctcacgg cccaggtcaa
     4141 ggattacctg gcccagcagg
//
LOCUS       DAOQLS010000103         4146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10584_length_4146_cov_4.984600, whole genome shotgun sequence.
ACCESSION   DAOQLS010000103 DAOQLS010000000
VERSION     DAOQLS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10584_length_4146_cov_4.984600"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..622)
                     /gene="tmk"
                     /locus_tag="PK362_02860"
                     /pseudo
     CDS             complement(71..622)
                     /gene="tmk"
                     /locus_tag="PK362_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
     gene            complement(792..2234)
                     /locus_tag="PK362_02865"
     CDS             complement(792..2234)
                     /locus_tag="PK362_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73814.1"
                     /translation="MESNRGKKAQSMVHHSNSSRKESGQVLIGAFVVVAVLLIFVPLM
                     VQWGKNESKWSTRDQMTMVAFNGADGAIDRGVWKLKSSTSTWAQASTGAVIAGYDFDT
                     TYTDLPGINYRIRFSSGPSAQQVTVVAEAKDVNTGQVRAIRASFQNMAFPGGVFTGGS
                     IGYSDDFAAHWGPVMAHGNINISGTAAGEYFPRKFSKQVVQKTGGVGNNRDTNGLNPP
                     NTDNLEWWSDYPCPDLPVLDFTTMRASASVNSTLNYRTNDNTSGSGKCVGWTVGSHNN
                     CQSGGATAASHYSATVCHFHDSNHHPRSKDNLIWYWDSNYDVLLTGKGTLTGHSNGIK
                     GTMIVRGNLTIADEDNLVSTVAIPSGAWREYAAIDTAAANQYPGDTGFQSQAATFGLG
                     TQTWTGGPASGAYSDVGILGFLYVGGNLSITAPYDHAGSIWVVGNVTTTSSERTYVFY
                     LGNLPNVPVLNVVLVRQSWDEVRPSTATWM"
     gene            complement(2271..2789)
                     /locus_tag="PK362_02870"
     CDS             complement(2271..2789)
                     /locus_tag="PK362_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC73815.1"
                     /translation="MSTLPGRAPLPSRTTRGYTLVEMMIVVSIIGMLIAMVAPLIIQT
                     QRFFILNRARVNIQREARSAMTTISKNLRQAQSLTIRLDNVTGQPYYSRIRFTTVDGR
                     ALTYYQSGTNLFQIGTSTSTLSTHVRFLNFFFPRTSDMSIVSISMTLEEVIYEGRKKA
                     LHVASERIRIMD"
     gene            complement(2770..>4146)
                     /locus_tag="PK362_02875"
     CDS             complement(2770..>4146)
                     /locus_tag="PK362_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNC73816.1"
                     /translation="MQQMTGFQAGEQFTREVSTAGYNHATLGMIADLYGRAYDHDWNL
                     DNIMTNGEVGAILPPHNSASAVRMTVTGTPAIGAVVSCNDGLSGPVTVPDSGWFWNGT
                     DNRWYRNPGFTMPSIATGTWSVYFSSTTALADSFVEVSSVVIVGGAALNIPHATTNPA
                     WEVPGRYSVFVTSSSEEGFVLGTVRNVFGTGIAPAITVTNNLASVLANTASPYRFFLR
                     TVPGIYNMTANANNANANYVSAVQTGVNVNAGQVTSGVDFILSQAGSLRGFVSRDGTN
                     ALPGIAMTATNAAGLVETTVVTGNDGRFTMNNMSTGAYVIEAALGSGEASAPLSVSTT
                     VTVGTSLWVGSFTVVGAAGYVRGSVTESGSPIKTGVMIVVSTAIVGAPPALSSASMTG
                     IPYYVGASYEDGTYALEVKGSTTTNFRIYAFYPVRNATGTWSTLTASSASVQILPGQT
                     KTGVNFAW"
BASE COUNT          754 a         1231 c         1355 g          806 t
ORIGIN      
        1 cgggcggccc gaaggccgcg atccacggga acatccagga gaaacgtcag attcgacggg
       61 ggtgttgccg ggccagggcc cggtagccgg cccgcacgcg ctcgtggaaa gccaggggct
      121 cccgttccag ccggtcgccg gccaaacggc gcccgccttt ggaaatcgtt ttggtgaggg
      181 atcgggcggc ccgaaggccg cgatccacgg gaacatccag gagaaacgtc agattcggcg
      241 ccaggccgcc ggtggccgta tcgttcaatc gggcgatttg gcggcggtcc aacccccggc
      301 cgaacccctg gtaggcgacg gtcgcgtcgg tgtagcgttc gcacacgacc accgcccccc
      361 ggtccagagc gggacgaata atttcggcca cgtgctgggc ccgggccgct tcgtagagga
      421 ggagttcggt catgggcgcg acccggccgc cgggccgcaa caggatccgg cgtacggcct
      481 cggcgatggt ggtgcccccg ggttcccggg tgtggaccac ccgccgaccc gcgcggcgga
      541 gggattggac cagaaggcgg gcttgcgtgc ttttacccga accttcgccg ccttccaggg
      601 tgatgaaaaa accgtgtttc acggaaattc tcccgttgcc ccgggcggag cccgaagaaa
      661 gtccgccttt cctccttggc cgtcaatgga ctcgggcaag ggacgacccg cgataggggg
      721 gccgttatcg acggtgggaa tctaccatat cccggaggga gggtcaaaaa gccgcggggc
      781 gcggcgggga attacatcca ggtggcggtg cttggacgga cctcgtccca ggactgccga
      841 acgagcacca cgttcagcac cgggacgttg ggtagattgc cgaggtaaaa cacgtaggtg
      901 cgttcgctgg aggtcgtggt gacgttcccg acgacccaga tgcttcccgc gtggtcgtag
      961 ggcgcggtga tggacaggtt gccgcccaca tacagaaacc cgaggatgcc cacgtcggag
     1021 taggcgcccg aggccgggcc gccggtccag gtctgcgtgc ccaggccgaa agtcgcggct
     1081 tgggattgga aaccggtgtc cccggggtat tgattggcgg cggcggtatc gatcgcggcg
     1141 tactcgcgcc aagcccctga cggaatggcc accgtggaaa ccaggttgtc ttcatcggcg
     1201 atggtcaggt tgccccggac gatcatcgtg cctttgatgc cgttgctgtg gccggtgagc
     1261 gtcccttttc cggtcaaaag cacgtcgtag ttggagtccc agtaccagat caaattgtct
     1321 ttggagcgcg gatggtggtt cgagtcgtgg aagtggcaga cggtggcgct gtagtgggac
     1381 gccgcagtgg cgccgccgct ttgacagttg ttgtgactcc ccacggtcca gccgacgcac
     1441 ttgcccgagc cggaggtgtt gtcgttggtc cggtaattca gagtgctgtt cacggacgcc
     1501 gaggccctca tggtcgtaaa atccaggacg gggaggtccg ggcaggggta atcggaccac
     1561 cattccaggt tgtcggtgtt gggggggttc aagccgttgg tgtcgcggtt gttgccgacc
     1621 ccgcccgttt tttgcacaac ctgtttggag aactttcgcg ggaaatattc gccggcggcg
     1681 gtgcccgaga tgttgatgtt cccgtgggcc atgacggggc cccaatgggc cgcgaagtcg
     1741 tccgaatagc cgatggaacc tcccgtaaac accccccccg gaaacgccat gttttggaag
     1801 gaggcccgga tagcccggac ttggcccgtg ttcacgtcct tggcttcggc aacgacggtc
     1861 acttgctggg ccgagggccc gctcgaaaac cgaatgcggt aattgatgcc cggaaggtcg
     1921 gtgtaggtcg tgtcgaaatc gtaccccgca atgaccgctc ccgtgctcgc ttgggcccag
     1981 gtggaggtgg aactcttcag tttccaaacg ccccggtcga tggcgccgtc ggccccgttg
     2041 aacgccacca tggtcatttg gtcccgggtg gaccatttgg actcgttttt cccccactgc
     2101 accatgagcg ggacaaaaat gaggaggacc gccacgacga caaaagcgcc gatcaggacc
     2161 tggccggatt cttttcgact tgaatttgaa tgatggacca tggattgggc ctttttcccc
     2221 cgattgctct ccattagttt aacccccgac aagcgttctt tcaaggtccg tcagtccatg
     2281 attcgaatgc gttcggacgc cacgtggagc gcctttttcc gcccctcgta aatgacctcc
     2341 tccaaagtca tcgatatcga cacgatggac atgtcgctgg tccggggaaa gaaaaaatta
     2401 agaaaccgca catgggtcga taaggtcgaa gtgctggtgc cgatttggaa gagattggtg
     2461 ccgctttgat aatacgtcaa cgcccggccg tccaccgtcg tgaagcggat gcgggaatag
     2521 tagggttgtc ccgtcacgtt atcgaggcgg atggtcaatg attgggcctg gcggagattt
     2581 ttcgatatgg tcgtcatggc cgaccgggcc tcgcgctgga tgttgacccg ggcgcgattg
     2641 aggatgaaga aacgctgggt ttggataatg agcggagcga ccatcgcgat cagcatcccg
     2701 atgatggaca ccacgatcat catttcaacg agggtgtacc cccgagtcgt ccgactcggg
     2761 agcggcgctc taccaggcaa agttgacacc cgttttcgtt tggccgggca ggatctgcac
     2821 gctggcgctg ctggccgtca acgtgctcca ggtgccggtc gcgtttcgta ccgggtagaa
     2881 cgcgtagatt cggaagttgg tcgtggtgct ccccttgact tccagggcgt aggtgccgtc
     2941 ttcgtaactg gcgccgacgt agtaggggat gccggtcatg ctcgcgctgc tcagggccgg
     3001 gggcgctcca acgatggcgg tggacacgac gatcatgacc cccgtcttga tcggacttcc
     3061 cgattccgtc acgctcccgc gcacgtaacc ggccgctccg accaccgtga acgaaccgac
     3121 ccacaaggag gtgcccaccg tcaccgtcgt ggacaccgac aacggcgcgg acgcctcgcc
     3181 ggaccccagg gcggcctcga tcacgtaggc gcccgtggac atgttgttca tggtgaagcg
     3241 gccgtcgttt ccggtgacca ccgtcgtttc cacgaggccg gccgcgttgg tcgcggtcat
     3301 ggcgatgccc ggcaaagcgt tcgtgccgtc gcgcgagacg aacccccgaa gggagcccgc
     3361 ttgggaaagg atgaaatcca ccccgctggt cacctgcccc gcgttgacgt tgaccccggt
     3421 ttggacggcg ctaacgtaat tggcgttggc gttgttggcg ttggccgtca tgttatagat
     3481 gccggggaca gttcgcaaga aaaaacggta gggtgacgcg gtgttggcca acacgctcgc
     3541 caagttgttc gtgacggtga tcgccggggc aatcccggta ccgaacacgt ttcgcaccgt
     3601 tcccaggaca aatccctcct cggacgagct ggtgacgaaa acggagtaac ggcccggcac
     3661 ctcccacgcg ggattcgtcg tggcgtgggg aatgttgagc gccgccccgc ccacaatcac
     3721 gacgctggat acttccacga aactgtccgc taatgcggtc gtggaactga aatacacaga
     3781 ccacgtcccc gtggcgatgg agggcatagt gaatccgggg tttcgatacc agcgattgtc
     3841 cgttccgttc caaaaccagc cgctgtccgg aacagtgacc gggcccgaca acccgtcgtt
     3901 gcagctcacg accgcgccga tcgccggggt tcctgtcacc gtcatccgga ccgccgaggc
     3961 gctgttgtgg ggcggaagga tagcgccgac ttcaccgttt gtcataatgt tgtccaagtt
     4021 ccagtcatga tcgtaggcgc gtccataaag gtcggcgatc attccaaggg tggcgtggtt
     4081 gtatcccgcc gtgctgacct cccgggtgaa ttgctccccc gcttgaaaac cggtcatttg
     4141 ttgcat
//
LOCUS       DAOQLS010000104         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10641_length_4134_cov_3.648934, whole genome shotgun sequence.
ACCESSION   DAOQLS010000104 DAOQLS010000000
VERSION     DAOQLS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10641_length_4134_cov_3.648934"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4134
                     /locus_tag="PK362_02880"
     CDS             <1..>4134
                     /locus_tag="PK362_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73817.1"
                     /translation="VLAPPYVEYALAIHRVVNPHNREDFTAVYGGAGADVSNAFPALN
                     PTNAYFISLYPRWLTADVLRRKFAAGLDAEERETLEEYRRAKEKFGFSRADWLETQDR
                     VLLALVYELEGLGVQADTVRVGETAGGRPVLTFPWRHPTEAVARPRRLVLVNADVTRP
                     DTYADVLDEPGFAVDAYFQNAGLEIPRSYLAHDESFLARVIAPRAGPRGLFFVTDDVI
                     HSNVHGVLDYEGAFPILRAERRLVPEESRWMREIIQTRQGSLEPDGSGRFNDAVSYGA
                     FKRVRFLPAGTGGRIREGGSWLLAAGALWAALVALPGLLTSVVAVGAAAFALGRKSRG
                     LPGPVMGLGRWGALAAAFAAGLFWSPDVWAAVAGGGNGFNAIWAMAPALALVVGGRGT
                     EPPGGPLYDFKKDRLNWDLFWSTIESAARSTEVPVNGHTEAGRPYLVPGDWDGRGCPT
                     ELRILLVYDFSSTGAAPLQMPRETVAAFLGRIAQSLSAAGEVSVGESTFVPEGVEARI
                     HLRSKGGREFKTIHLKVRVTGDRPARAYLAEIRADAGSDFWSSVRSLLRLERAFGDPA
                     VHGAWMEKYVQAVRLKRVGRFFKEDAEVQSRRRAWTARAKDLLAHPTRLTREEASRLE
                     GVLRDSLESLRAAPPTTPIPATDNLRTDKEGRREFLDRMFDALSLDVQLTTDELPGEF
                     RDALERFNARGWAFYPSATGRVDVWSGERGRYFRFVRLTGAPRLALRWVWRPGEGLLL
                     YGERPGGDPAVFSIRRYAETLAGPQETVVLSPLPSADFAGLRERSREEDLRERLTLLL
                     REATDPALPFTKDRARAFEGFAAEGYEGKIYVRRKTDRGDNRFFYVTLRKGQRLNFQS
                     TLFDGDRARVTPMAFPGQDAVVGLTPPGGRTLTFSVGPAVGAPPQKSEINAEGLWSFD
                     SPDQAREELAAHLEFSSFLLRWGPRPPAELVDALSDEALAAQNAAAPLVRVRAPGEAY
                     EIHGARTRRLLRFNGLAVGNYRPRVERDSVGLKIVFVNTNPHGASLPFRVPPFPAEGD
                     VRNFYSSGEGGVAAVEKSMLTDALEPLIQWALTPEALEGGVEPENRRRERRAFNDRAF
                     TVTTDESGRLPFSFSEAVPAPRKISAPEGVLPPRATYRLSLEWLWGAGPVLALSRRGD
                     ARLFAFGRWRRVWASGQKTVAMDGVAEGTSLSGALAAAGRSPALLLRQGERLRQRPNE
                     GDLADYRRRTERFNAVEAPQNNKRSGHALLDDPFDGTRWVFRGLGDTELQYRRFIEWR
                     PGIGPVVYFLYDGSLKLRSGRVKVFYDLFRARAWAGVGAEEIDVWALKRARGETLEAS
                     RAAVTQFVEDLAADPVFGRGNDPSTEGMARFLRWVAGRWAESLGGAAAVPPVWPLWSP
                     "
BASE COUNT          601 a         1353 c         1421 g          759 t
ORIGIN      
        1 gttctcgctc cgccctacgt ggaatacgcc ctggccatcc accgcgtggt caacccccac
       61 aaccgcgagg actttacggc cgtttacggc ggtgccgggg cggacgtgtc caacgccttt
      121 cccgctctga acccgaccaa cgcgtacttc atctccctgt atccccggtg gctcacggcc
      181 gacgtccttc ggcggaaatt cgccgcgggt ctggacgccg aggaacggga aaccttggag
      241 gaataccgcc gggcgaagga aaaattcgga ttttcccggg cggattggct ggaaacccag
      301 gaccgggttc ttctggccct ggtctacgaa ctggagggcc tgggcgtcca ggccgacacc
      361 gtccgcgtgg gggaaacggc cggcggccga cccgtattga cgttcccctg gcgtcacccg
      421 accgaggcgg tcgcccgacc gcgacggttg gtcctggtga acgccgacgt cacccggccc
      481 gacacctacg ccgatgtgct ggacgagccc gggttcgccg tcgacgccta ctttcaaaac
      541 gccgggttgg agatcccccg gtcttatttg gcccacgacg aatcgttcct ggcccgggtc
      601 atcgctcccc gcgcggggcc ccgggggctc tttttcgtca cggacgacgt catccattcg
      661 aacgttcacg gcgtgttgga ttacgaaggc gccttcccga tccttcgcgc cgagcggcgt
      721 ttggttcccg aagaatcccg gtggatgcgg gaaatcattc aaacgcggca gggctcgctg
      781 gaaccggacg gaagcggccg tttcaacgac gcggtgtcct acggcgcctt caagcgggtc
      841 cggtttttgc ctgccgggac gggcgggcgg atccgcgaag gcggatcctg gctgttggcg
      901 gccggggccc tctgggccgc cctggtcgct cttcccggcc tcttgacctc ggtggtggcg
      961 gtgggtgccg ccgccttcgc ccttgggcga aaatcccggg gcctcccggg accggtgatg
     1021 ggactcggcc gttggggggc cctggccgcc gctttcgccg ccggattgtt ttggtccccg
     1081 gacgtctggg ccgccgtggc gggcggcggg aacggcttca acgcgatttg ggccatggcg
     1141 ccggccctgg ccctggtcgt cggcggccgg ggaacggaac ctccgggggg gcccctgtac
     1201 gatttcaaaa aagaccggtt gaattgggac ctcttttggt cgacgatcga atcggccgcc
     1261 cggtcgacgg aagttccggt caacggccac acggaggccg gccggccgta tctcgtcccc
     1321 ggcgattggg acggccgggg ttgtcccacg gaacttcgaa tcctcctggt ctacgatttt
     1381 tcttccaccg gggcggcgcc ccttcagatg ccccgggaga cggtggccgc ctttttggga
     1441 cggatcgctc aatccttgtc cgcggcgggc gaagtgtcgg tgggcgaatc cacctttgtt
     1501 cccgaaggcg tcgaagcccg gattcacctc cgttcgaagg gcggccggga attcaaaacc
     1561 attcatctca aagtgcgcgt cacgggggat cggccggcgc gggcctattt ggcggaaatc
     1621 cgggccgacg ccggatcgga tttttggtcg tcggtgcgaa gcctcctgcg gttggagcgg
     1681 gccttcggcg atccggccgt tcacggcgcg tggatggaaa aatacgtcca ggccgtccga
     1741 ctgaaacggg tcggtcgttt cttcaaggaa gacgccgagg tccaatcccg ccgccgggcc
     1801 tggacggccc gtgcgaagga ccttttggct catcccaccc gcttgacccg ggaggaggcg
     1861 tcgcggttgg agggcgttct tcgcgattcg ctcgagtccc tccgcgccgc ccccccgacg
     1921 acgccgatcc ccgcgaccga caaccttcga accgacaagg agggacgccg ggaattcctg
     1981 gatcggatgt tcgacgcgtt gtccctggac gtccaattaa cgacggacga attgcccgga
     2041 gaattccggg acgctctgga acgcttcaac gcccggggtt gggcgttcta cccctcggcc
     2101 acggggcggg tggacgtctg gtcgggggaa cgggggcgtt atttccgttt tgtccgtctg
     2161 accggcgccc cgcgcctggc ccttcgctgg gtgtggcggc cgggggaagg gttgctcctg
     2221 tacggcgaac gtcccggcgg cgacccggcg gttttttcca ttcgacggta tgccgagact
     2281 ctggcgggtc cccaggaaac cgtcgttctt tcgcccctgc cgtccgcgga cttcgcgggc
     2341 cttcgggagc ggtcccggga ggaagatctt cgggaacgac tgacccttct ccttcgcgag
     2401 gccacggacc cggcgttgcc gtttacgaag gaccgggccc gggcctttga ggggtttgcc
     2461 gcggagggat acgagggcaa aatctacgtc cgccggaaga ccgatcgtgg ggacaaccgc
     2521 tttttctatg tgacccttcg caaaggccag cggttgaatt ttcaaagcac cctctttgac
     2581 ggggaccggg cgcgggtcac ccccatggcc ttccccggcc aggacgcggt ggtgggcctc
     2641 accccgcccg gggggcgaac gctgaccttt tccgtggggc ccgccgtcgg ggctcccccg
     2701 caaaaatcgg aaatcaacgc cgaagggctg tggtccttcg attccccgga ccaggcccgg
     2761 gaggagctgg ccgcccacct ggaattctcc tcgttcctcc tccgctgggg cccccgcccg
     2821 cccgccgaat tggtggacgc cttgtccgac gaggccctgg ccgcccaaaa cgcggccgcc
     2881 ccgctggtcc gtgttcgcgc cccgggggag gcctacgaga ttcacggcgc ccgcacccgc
     2941 cgcctgcttc ggttcaacgg actcgcggtg gggaattacc gcccgcgggt ggaacgggat
     3001 tcggtggggt tgaaaatcgt ttttgtgaac acgaatccgc acggcgcctc cctgcctttc
     3061 cgggttcccc ctttcccggc ggagggggac gtgcggaatt tttacagttc cggcgaaggg
     3121 ggggtcgccg cggttgaaaa atcgatgctc accgacgccc tcgaaccgtt gattcaatgg
     3181 gccctcacgc ccgaggctct ggagggcggc gtcgagcccg aaaaccgtcg gcgggaacgc
     3241 cgggcgttta acgatcgggc ttttacggtc accaccgacg aatccggccg actgcctttt
     3301 tcattttcgg aagcggttcc cgctccccgg aaaatctccg cccccgaagg cgtccttccg
     3361 ccccgggcga cgtaccgatt gtccctggaa tggctgtggg gggccggtcc ggtcctggcc
     3421 ctgtcccgcc ggggagatgc ccgtctgttc gccttcggtc gctggcgccg ggtgtgggct
     3481 tcggggcaga aaaccgtggc gatggatggg gtggcggagg ggacgagcct ctcgggcgcc
     3541 ctggcggcgg cggggcggag cccggccctg ctccttcgcc agggcgagcg gcttcgccag
     3601 cgacccaacg aaggggacct cgccgactac cgccggcgca ccgaacgttt caacgccgtg
     3661 gaggcgccgc aaaacaacaa acgaagcggg cacgccctgt tggacgatcc ctttgatgga
     3721 acgcgctggg tgttccgggg attgggcgac acggaacttc aataccggcg gtttatcgag
     3781 tggcgtccgg ggatcggtcc cgtggtgtac ttcctctacg acggttccct gaaattgcga
     3841 tcgggacggg tcaaggtttt ttacgatctc ttccgagccc gggcctgggc cggcgtcggg
     3901 gcggaggaga tcgacgtgtg ggcgctaaaa agagcccggg gagaaaccct ggaagcctcc
     3961 cgggccgccg tcacgcaatt cgtcgaagac ctggccgcgg atccggtttt tgggcgggga
     4021 aacgacccct cgaccgaggg gatggcccgg tttcttcgtt gggtggccgg acgctgggcc
     4081 gagtccctgg ggggggcggc ggccgttccc ccggtgtggc ccctctggag tccc
//
LOCUS       DAOQLS010000105         4133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10649_length_4133_cov_4.958803, whole genome shotgun sequence.
ACCESSION   DAOQLS010000105 DAOQLS010000000
VERSION     DAOQLS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10649_length_4133_cov_4.958803"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK362_02885"
     CDS             complement(<1..556)
                     /locus_tag="PK362_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73818.1"
                     /translation="MKRSIALFALMSLFALSGLRAEDATGRLGLGGAFGLDDAYARKV
                     LRRGTDADVFGSAWLRFGFSQKNELALAYDNLQLKPKGAAAAGRSIVQPVTLNLWHSV
                     SPDRRLTPFWVVGAGVADLRRMDVSRRTGLALQAGGGLEYFVCRGFSLGALVRAHYVA
                     NRHSNGTTEGTLLSAGLMANLFWPA"
     gene            complement(585..1007)
                     /gene="mscL"
                     /locus_tag="PK362_02890"
     CDS             complement(585..1007)
                     /gene="mscL"
                     /locus_tag="PK362_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNC73819.1"
                     /translation="MSLVKEFKEFAMRGNVVDMAVGVVIGGAFGKVVSSFLNDVVMPP
                     LGMLTGGVDFTDKAVTLKPAAEGAKAVTLNYGSFITTCVDFVIVAFAIFVVVKIMNSL
                     KKKEPVAAPAPTEKACPQCLLTIPIAAKKCGHCTSVIA"
     gene            complement(1086..2261)
                     /locus_tag="PK362_02895"
     CDS             complement(1086..2261)
                     /locus_tag="PK362_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC73820.1"
                     /translation="MRRSLFPLVLILVGSPLFAQDIRERWGVGGAVGYGDYLSPGSAK
                     KGTNADLYLSGWVREGVGSNTELIAALDGLSATGSSVRSGLRARALTLGLLQPLIRPD
                     DWVPYVVLAGGAAFVHAPGAVRPDVVQPVGKFGLGLERYVGAKSTLGLQINYHYLLSN
                     GRYAPESSAASVALTYTSFFYCGLGAQRVLPTPAAVPPPNATPVYSHPTAVIAQDTDG
                     DGVPDLRDNCPDTPEGQTVDAFGCPKDSDGDGVLDDLDACPNTPAGALVNSAGCPAET
                     VSVTLDIKFDAGQSVVRPEFDAQIGKVADFMARFPNTTVLIEGHTDNAGNPATSLPLS
                     QRRADAVRQTLVAKFHVAPGRVTAKGYGDTRPIANNKTPEGRAANRRVVATLSATKK"
     gene            complement(2386..2985)
                     /locus_tag="PK362_02900"
     CDS             complement(2386..2985)
                     /locus_tag="PK362_02900"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC73821.1"
                     /translation="MSDTVKIGQVVPDFQMDVYDPRSGEFGTFSLADARKKGQWTVLV
                     FYPADYTFVCPTELADVARQQDELAKAGAQVLGVSRDTKFVHLAWRREEKLLKDVRYP
                     LAADPTGAVSRLFGVLDEASGLAFRGTFIIAPDGKLTGSEVNFFNVGRSAEELLRKVK
                     ANVYLAKHPQEACPANWSEGAKTLKPGANLVGRVAEALA"
     gene            complement(3008..3478)
                     /locus_tag="PK362_02905"
     CDS             complement(3008..3478)
                     /locus_tag="PK362_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HNC73822.1"
                     /translation="MTNAAPSKSAAPSVVAEALARVLADSYRLYVKTQNFHWNVVGPH
                     FAPLHAQFQSQYEELAPAIDEVAERLRALGQRAPGSMKEFLALGTLPEAQNEELADDM
                     VRSLEKGHRALAQGAKAALDAAQRAGDDVTVDLMVERIAAHDKAAWMLASFLVH"
     gene            3580..>4133
                     /locus_tag="PK362_02910"
     CDS             3580..>4133
                     /locus_tag="PK362_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC73823.1"
                     /translation="MQELRYAAAVYRERHFVRAADRVHVSQPTLSQGLKKLERELGAP
                     LFDRSPRGVRTTPAGEKFLQRVLPALSVLESAAEEIKADAGEPTGVVRLGAIPTLGPY
                     LWPRALPLLRKKAPRLTVEIHEVTTSLLLAQLKDGRLDVGLLALPAGERGFAARSFGE
                     EPFCLAVDRNHPLARKKAVSVRDLA"
BASE COUNT          728 a         1262 c         1439 g          704 t
ORIGIN      
        1 cggcgggcca aaagaggttc gccatcaggc cggccgaaag gagcgttccc tccgtggtgc
       61 cgttggaatg gcgattggcc acgtagtggg cccggaccaa ggcgccgagg ctgaaaccgc
      121 ggcaaacgaa atattccaac ccgccgccgg cctggagggc cagtccggtg cggcgggaga
      181 cgtccatccg gcgcaagtcc gcgacgccgg cgcccaccac ccagaagggg gtgaggcggc
      241 gatcggggga cacggaatgc cagaggttca gggtcacggg ttggacgatg gaccgcccgg
      301 cggccgcggc gcccttgggt ttcaattgga ggttgtcgta ggcgagcgcg agctcgttct
      361 tttgggaaaa cccgaagcgg agccaggccg aaccgaacac gtcggcgtcg gtcccgcggc
      421 gcaggacttt gcgggcgtag gcgtcgtcca aaccgaaggc gccgcccaga ccgaggcggc
      481 cggtggcgtc ttcggcgcga aggccggaca gggcgaagag ggacatcaag gcgaacaggg
      541 cgatggatcg tttcatgaag ggctccttgg gggtcgggga aagcttaagc gatgacgctc
      601 gtgcagtggc cgcatttctt cgcggcgatc ggaatcgtca acaggcattg gggacaggcc
      661 ttttccgtcg gcgcgggggc ggcgacgggc tcttttttct tcaggctatt catgatcttg
      721 acgaccacga aaatcgcgaa ggcgacgatg acgaaatcca cgcaggtggt gatgaacgac
      781 ccgtagttga gcgtcacggc cttggccccc tcggcggcgg gcttgagggt cacggctttg
      841 tccgtgaaat ccacgccgcc cgtgagcatg cccaggggcg gcatcaccac gtcgtttaaa
      901 aaggacgaca ccactttgcc gaaagcgccg ccaatcacga cgccgaccgc catgtccacg
      961 acgttgccgc gcatggcgaa ttccttgaat tctttaacga gagacatatt ttcctccaac
     1021 ggatttttta aaaacggtga ccccgcccgg aaggggcgcc gaagacgtcg gttcaaccgg
     1081 gtcgattatt ttttggtcgc cgagagggtc gccacgacgc gtcgattggc cgcgcggccc
     1141 tcgggggttt tgttgttggc gatggggcgg gtgtccccgt agcctttggc cgtcacgcgc
     1201 cccggggcca cgtggaattt cgccacgagg gtttggcgca cggcgtcggc ccgccgctgg
     1261 gaaagcggca ggctggtcgc cgggttgccg gcgttgtccg tgtggccttc gatgagcacg
     1321 gtggtgttgg ggaaccgcgc catgaaatcg gccactttgc cgatctgggc gtcgaattcc
     1381 ggtctgacga cggattgccc ggcgtcgaat ttaatgtcca gcgtcacgct cacggtctcc
     1441 gccgggcaac cggcgctgtt caccagggcg cccgccgggg tgttggggca ggcgtccaaa
     1501 tcgtccaaaa cgccgtcccc gtccgagtcc ttgggacagc caaaggcgtc gacggtttgc
     1561 ccttccgggg tgtccgggca gttgtcccga agatccggca cgccgtcgcc gtcggtgtcc
     1621 tgggcgatga cggccgtggg gtgggaatag accggtgtcg cgttgggggg tggcacggcc
     1681 gcgggggtgg gcaacacccg ctgggcgccc agcccgcagt agaaaaagga cgtgtaggtg
     1741 agggccaccg aggcggcgct ggattccggg gcgtagcggc cgttcgacag aaggtaatgg
     1801 taattgatct gcaagcccag ggtggacttg gcgccgacgt agcgttccaa gccgaggccg
     1861 aatttgccca cgggctggac gacgtccggc cggaccgccc cgggggcgtg aacaaaggcc
     1921 gccccgccgg ccagcaccac gtagggaacc caatcgtcgg gtcgaatcag gggttgaagg
     1981 aggcccagcg tgagggcccg ggcgcgaagg cccgatcgga cggaactccc ggtggccgac
     2041 aggccgtcca gggccgcgat aagctccgtg ttggacccga cgccctcccg gacccagccg
     2101 gacaggtaca agtcggcgtt ggtccccttc ttggccgatc cgggggaaag ataatcgccg
     2161 tagccgacgg ccccgcccac gccccaccgc tcccgaatgt cctgggcgaa caggggagaa
     2221 ccgacaagga ttaaaacgag aggaaaaaga gaacgtcgca tgggggctcc tcggccgaaa
     2281 gacaccgacg gaattttagc gaacttcggg ggggaaggga agccccacgt ccgcgcgccc
     2341 cggcccgcgg ggggccgggg cggcgaaccc gggcggggcc ccgccttagg cgagggcttc
     2401 ggccacccgg cccacgagat tcgcgccggg tttcagggtt ttggcccctt ccgaccaatt
     2461 ggccgggcag gcttcctggg gatgtttcgc caggtaaacg ttggccttca ctttgcgcag
     2521 gagttcctcc gcgcttcggc cgacgttgaa gaagttgact tcggacccgg tcaacttgcc
     2581 gtccggcgcg atgatgaagg tgccgcggaa ggccaaaccg ctggcttcgt ccaaaacgcc
     2641 gaagaggcgg cttaccgcgc cggtggggtc cgcggccaag gggtaacgga cgtctttcag
     2701 caatttttcc tcccgccgcc aggcgaggtg gacgaacttg gtgtcccggc tgacgcccag
     2761 aacctgggcg ccggccttgg ccagttcgtc ctgctgtcgg gccacgtcgg ccagttccgt
     2821 cggacacacg aaggtatagt cggcggggta aaacacgaga acggtccact ggcccttttt
     2881 gcgggcgtcg gccagggaaa aagtgccgaa ttcccccgac cggggatcgt agacgtccat
     2941 ttgaaagtca gggacaacct gaccgatttt gacggtgtcg ctcatagtgc ctcctggaat
     3001 caacggatca atgaaccaaa aagcttgcca acatccaggc cgccttgtcg tgggcggcga
     3061 tgcgctccac catgaggtcg acggtgacgt cgtccccggc gcgctgggcg gcgtccaggg
     3121 cggctttcgc cccctgggcg agggcccggt gacccttttc cagggaccgg accatgtcgt
     3181 cggccaattc ctcgttctgg gcttcgggca gggtgccgag ggccaaaaat tccttcatcg
     3241 atcccggcgc ccgctgcccc agggcccgaa gccgttccgc cacttcgtcg atggcggggg
     3301 cgagctcttc gtactgggat tggaattggg cgtgcagggg cgcgaaatgg gggcccacca
     3361 cgttccaatg gaagttctgg gttttcacgt agagccggta actgtcggcg aggacccggg
     3421 cgagggcctc tgccacgacg gacggagcgg cggatttgga cggggcggcg ttggtcatgg
     3481 gattcctcct ggaatttctt gacctcattc tacgccgtgt cctacaatag atcaaatgga
     3541 ttgattaaat atatcatata ggagtattca ataatggaaa tgcaggaatt gcgttacgcc
     3601 gcggcggtgt atcgggagcg gcactttgtc cgggcggcgg accgcgtgca cgtcagccaa
     3661 cccacgttga gccaggggct gaaaaaactg gagcgcgaac tgggggcccc cctgttcgac
     3721 cgctccccgc ggggggtgcg gacgacgccg gcgggggaaa agttcctcca gcgggttctt
     3781 cccgcgctgt ccgtcctcga gagcgccgcc gaggaaatca aggcggacgc gggggagccc
     3841 accggggtgg tccggttggg cgcgatcccg accctgggcc cttacctgtg gccccgggcg
     3901 cttccgcttc ttcggaaaaa agccccgcga ctcacggtcg agattcacga agtcacgacg
     3961 tcccttcttc tcgcccaatt aaaggacggc cggttggacg tggggctcct ggccctgccc
     4021 gccggcgaac gggggttcgc cgcccggtcc ttcggcgagg agcccttttg cctggccgtc
     4081 gaccgaaacc acccgctggc ccggaaaaaa gccgtgtccg tccgggacct ggc
//
LOCUS       DAOQLS010000106         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10689_length_4125_cov_3.355283, whole genome shotgun sequence.
ACCESSION   DAOQLS010000106 DAOQLS010000000
VERSION     DAOQLS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10689_length_4125_cov_3.355283"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1760
                     /locus_tag="PK362_02915"
     CDS             99..1760
                     /locus_tag="PK362_02915"
                     /inference="COORDINATES: protein motif:HMM:NF006754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HNC73824.1"
                     /translation="MTDSWNFVNRVRDVARADPDRRALIFPKTKPGVSPVDYGTVTFK
                     ELNEESDRYAQGLRAFGIGRGTRVLLLVPVSVELLTLALALLKMGAILVLIDPGMGKK
                     SLLKCVQHVQPRGLIAVPLVHALRTLSRKSFGGITHAVTVGAKWWWGGPTHWDLRNPV
                     WTEFPVAAVSREELAAIVFTTGSTGLPKGVHYTHGVFDAQVKILEREFHAGDRVGLAA
                     FPTMALYYLAMGIPCVLPPVDPARVARVNPRDIVDAVERFGVTSAAGSPMFWDPVVRY
                     CRENGRRLESLKTVTMFGAPVRGSLLEEFAAVLPPGGRTFTPYGATEALPVSSLSGDE
                     ILAETWPRTQSGAGVCVGRPFAELTVKIIPISDEPIPDWDDARVLPPGAIGEITVKGP
                     VVTKRYDHHDRENIWAKIYEGSDVWHRMGDAGYFDDRGRLWFCGRKNHRVETAGGVLY
                     SVPVEEIFNVHPRVFRTALVGLGARPRQRPVLIVEPRSGAAPRNRSERTVFIDELKAL
                     AKNHPALGEVRDILFHPALPVDYRHNAKIIREELVPWAAKELGGG"
     gene            1764..2768
                     /locus_tag="PK362_02920"
     CDS             1764..2768
                     /locus_tag="PK362_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC73825.1"
                     /translation="MRALVTGGQGFLGLHLVRFLLGRGVAVRALVRRPAPEVEALGVE
                     CVVGDLRDPVAVRAACRDRDVVFHVAAHVALWGPWEDFFSVNVRGTENVLAGCRAEGV
                     PRLVYTSTPSVVFDNSNQEGVNESLPYPDVFLSPYPASKALAEKAVLAAHGRDGLCTV
                     ALRPHLIWGPDDPHIVGQLLKRARRGRLFLVGSGRNRVDVTYVDNCAEAHWLAAQALG
                     PGSRLGGRAYFLSQGRPVPIAEFMRRVADAAGLSPRLFRIPYPVAWAAGWLMEKFGGG
                     ESGVPLLTRFLAAELAKSHYYDIAAARTDFGYEPRITTDEGFRRLAASLASKSEGTTR
                     "
     gene            complement(2743..>4125)
                     /locus_tag="PK362_02925"
     CDS             complement(2743..>4125)
                     /locus_tag="PK362_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73826.1"
                     /translation="PPAKTPAPPAKPPAPPPAPRPPTPPLPEFIYGTAAAEEPEMGFK
                     DLKLAKFESHGVASEFWAYRMAHPRNTRRKRSELYLEQTMRPNDSLRLFFSERANIDF
                     AVRQRNRDSEGTPETEKDTNESTLEWREAYVDWSPGLFTVRVGAQQIVWGEAVGLFVA
                     DVVNARDFREMILPDTWLIRRPQWALDVVHNGGLFSQEFVLLVPRANRWPAVDSDFFF
                     LPDEAGPLVRGVDLTTIPEQPLPVSLNTLESGGRLSMLAGGLDLSVFFLHSFEKDPVY
                     FRIPNEANFTGDFEIRHPRQNYYGFTFSKDVSDAIVKGEMVAKPRRYTAIDNPDDEDN
                     VDRRSAYDAMVGVDYTFFDNWDTNLQLIDRYVPGFSEDLIRVEKNRLYVAGRVSRKFV
                     NNKLECELIVLHHFGKGDEILRPKMLYKMSDWVTFKVGADVFSGNDPEGIFSVYNRQD
                     RVFSELSLHF"
BASE COUNT          684 a         1304 c         1346 g          791 t
ORIGIN      
        1 gtcgtggacc gatttcccgg ggcgggccat tacgtgttgg aggacgccca cgaattcatt
       61 ttgccccgcc tgagggaatt tttggacgct ccggccacgt gacggattcc tggaatttcg
      121 tcaaccgcgt tcgcgacgtc gcccgggccg acccggaccg gcgcgccttg attttcccca
      181 agaccaaacc gggcgtttcc cccgtcgatt acggcacggt gaccttcaaa gagttgaacg
      241 aggagagcga ccgttacgct cagggccttc gggccttcgg catcgggcgg gggacccggg
      301 tgttgttgct ggttcccgtc agcgtcgagt tgttgaccct ggccctggcg cttctcaaaa
      361 tgggggcgat cctggtgctg atcgatcccg ggatgggaaa gaagagtctt ctcaaatgcg
      421 tgcagcacgt tcagccccgg gggttgatcg ccgttcccct cgtccacgcc ctgcggaccc
      481 tgtcgcgcaa atccttcggg ggcatcaccc acgccgtcac ggtgggggcc aaatggtggt
      541 ggggggggcc cacccactgg gatttgcgga atcccgtgtg gaccgaattt cccgtggccg
      601 ccgtgagccg ggaagaactg gccgccatcg tctttacgac gggcagcacc ggcctcccca
      661 agggggtcca ttacacccac ggggtgttcg acgcccaggt caaaatcctg gagcgggaat
      721 tccacgcggg ggaccgcgtg ggcctcgccg cgtttcccac catggctctc tattacctgg
      781 ccatggggat tccctgcgtg ttgccgccgg tggacccggc gcgggtcgcc cgggtgaatc
      841 cccgggacat tgtcgacgcc gtggaacggt tcggcgtcac cagcgccgcc ggttccccga
      901 tgttttggga tcccgtggtc cgctattgcc gggaaaacgg ccgccgcctg gaatcgctga
      961 aaaccgtcac gatgttcggc gccccggttc gggggtccct cctggaggag tttgccgcgg
     1021 ttctcccgcc gggaggccga accttcacgc cgtacggcgc cacggaagcc ctcccggtat
     1081 cctccttgtc gggggacgaa attttggccg agacctggcc ccgaacccaa tccggcgcgg
     1141 gcgtttgcgt gggccggccg tttgcggagc tcaccgtgaa aatcatcccc atctcggacg
     1201 aacccattcc cgattgggac gacgcccggg ttttgcctcc cggggccatc ggcgaaatca
     1261 cggtcaaggg gcccgtggtc acgaagcgct acgaccacca cgatcgggaa aacatttggg
     1321 ccaaaattta cgaagggtcc gacgtctggc accgcatggg cgacgccggg tacttcgacg
     1381 accggggacg gctgtggttc tgcggccgca aaaaccaccg ggtggaaacc gccggcggcg
     1441 tgctgtattc cgtgccggtc gaggagatct tcaacgttca tccccgggtg tttcgcaccg
     1501 cgctggtcgg cctcggcgcc cgtccccggc agcgccccgt gctgatcgtc gagccccgtt
     1561 ccggagccgc cccccgaaac cggtcggagc gaacggtgtt catcgacgaa ctgaaggcct
     1621 tggccaaaaa ccacccggcc ctgggggagg tccgcgacat tctttttcat cccgccttgc
     1681 ccgtggatta ccggcacaac gcgaaaatca ttcgcgaaga attggttccc tgggcggcga
     1741 aggaattggg cgggggctga tcggtgcggg cgctggtcac cggcggccag ggttttctgg
     1801 gccttcactt ggttcgtttc cttctcggac ggggcgtcgc cgtgcgggcc ctggttcgcc
     1861 gcccggcgcc ggaggtggaa gccctcgggg ttgaatgcgt cgtgggggac ctgcgggacc
     1921 cggtcgccgt tcgcgccgcc tgccgggacc gcgacgtggt gttccacgtg gcggcccacg
     1981 tggcgctctg gggtccctgg gaggactttt tctccgtcaa cgttcgcggg accgaaaacg
     2041 tcctggccgg ttgccgcgcc gagggcgtgc cccggctggt ctacaccagc acgccgagcg
     2101 tcgttttcga caattcgaac caggagggag tcaatgaatc cctcccctac cccgacgttt
     2161 ttctgagtcc ctaccccgcg tccaaagccc tggccgaaaa agccgttttg gccgcccacg
     2221 gccgggacgg tctctgcacg gtggcgctcc ggccccatct gatttggggg cccgacgacc
     2281 cccacattgt cgggcagttg ctaaaaaggg cccggcgcgg tcgtttgttt ttggtgggca
     2341 gcggacggaa ccgggtggac gtgacttacg tggacaactg cgcggaggcc cattggctgg
     2401 cggcccaagc cctgggtccg ggttcccgcc tgggggggcg cgcgtatttc ttaagccagg
     2461 ggcgccccgt gcccatcgcg gaattcatgc ggcgggtggc cgacgccgcc ggactttcgc
     2521 cccgcctatt ccgcatcccc tatcccgtgg cctgggccgc gggctggctg atggaaaaat
     2581 tcggcggggg ggaatccggc gttccgctcc tcacccgctt tctcgcggcc gaattggcca
     2641 aatcccatta ctacgacatt gccgccgcgc ggaccgactt cggttacgaa ccgcgcatca
     2701 ccaccgacga gggattccgc cggctggcgg ccagtctggc gtctaaaagt gaagggacaa
     2761 ctcgctgaac acccggtctt ggcggttgta gacgctgaaa atcccttcgg gatcgttccc
     2821 cgaaaacacg tcggccccca ccttgaacgt cacccaatcg gacattttat aaagcatttt
     2881 cggccgtaga atttcgtcgc cttttccaaa gtgatggagg acgatcaact cgcactccag
     2941 cttgttgttg acgaatttgc ggctcacccg gcccgccacg taaaggcggt ttttctcgac
     3001 ccggatcaaa tcctcggaaa accccggcac gtaccggtcg atcaattgaa ggttcgtgtc
     3061 ccaattgtcg aagaaggtgt aatccacccc caccatggcg tcgtacgccg agcggcggtc
     3121 cacgttgtcc tcgtcgtcgg ggttgtcgat ggccgtgtag cggcggggct tggcgaccat
     3181 ttcgcctttc acgatggcgt cggacacgtc cttggaaaac gtgaagccgt aataattctg
     3241 gcggggatgg cggatctcaa aatcgcccgt gaaattggcc tcgttgggaa tccggaaata
     3301 aaccggatct ttctcgaaag aatgaaggaa gaacaccgat aggtcgaggc cgccggccag
     3361 catggacagt cggcccccgg attccagggt gttgagggaa acgggaaggg gttgttccgg
     3421 tatggtcgtg aggtccacgc cccgcaccaa ggggccggcc tcgtcgggga gaaagaaaaa
     3481 gtcggagtcc accgccggcc agcggttggc ccggggaacg aggaggacga attcctggga
     3541 gaacaaaccg ccgttgtgca ccacgtccag ggcccattgg ggacggcgaa tgagccaggt
     3601 gtcgggcaaa atcatttcgc ggaaatcccg ggcgttcacc acgtcggcca cgaagaggcc
     3661 cacggcctcg ccccaaacga tctgctgggc gccgacccgg acggtgaaga ggcccggcga
     3721 ccaatccacg taggcctccc gccattccaa ggtggattcg ttggtgtctt tttccgtttc
     3781 cggggtgcct tcgctgtccc ggttccgctg gcggaccgca aaatcgatat tggctctctc
     3841 ggaaaaaaac aatcgcaggc tgtcgttggg ccgcatcgtt tgttccaggt acaattcgct
     3901 cctttttcgc cgggtgttgc gggggtgggc catccgatag gcccagaact ccgacgccac
     3961 gccgtgggat tcgaatttgg ccaatttcaa atccttgaac cccatttccg gttcctcggc
     4021 ggccgcggtc ccgtagatga actccggtaa gggcggcgtc ggcggccggg gcgcgggggg
     4081 cggcgccgga ggtttggccg gcggggccgg cgttttggcg ggcgg
//
LOCUS       DAOQLS010000107         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10850_length_4093_cov_4.190193, whole genome shotgun sequence.
ACCESSION   DAOQLS010000107 DAOQLS010000000
VERSION     DAOQLS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10850_length_4093_cov_4.190193"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PK362_02930"
     CDS             complement(<1..1424)
                     /locus_tag="PK362_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC73827.1"
                     /translation="MTLSKKNLVSSEKAFLIGGVWRGAGPGGGAFENFDPATGRRLNV
                     YPAASPSDVDDAVAAAKKAYGAWRKMPVPRRGDILLKSMRLLEERKEKFARAMTREMG
                     KVLTETRGDVQEAIDTAFYYAGEGRRLFGQTTTSELPDKFAMSLRQPVGVCALISPWN
                     FPMAIPSWKIYPALLCGNTVVLKPASYTPDSARNFVQTLEEAGVPPGVVNIVFGNGSE
                     VGQRLIAHPDVRLVSFTGSSSVGSNVGEVAGRLYKKCSLELGGKNAQIVMDDADLDLA
                     LEGALWGAFGTSGQRCTATSRILVHKAVLKTFTDRFVAASKKIKIGNGLEDGVQMGPL
                     ISARQLESVQNYVKIGQKIDRAVLLTGGRVLTTGRYAGGHYHEPTVFLGNPTMRIARE
                     EIFGPVTTIVPVHSFEESIAVLNDTEYGLSGSLYTRDIRRAMRAVRDMETGIAYINAP
                     TIGAEVHLPFGGVKNTGNGHREAGV"
     gene            complement(1595..2125)
                     /gene="msrB"
                     /locus_tag="PK362_02935"
     CDS             complement(1595..2125)
                     /gene="msrB"
                     /locus_tag="PK362_02935"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNC73828.1"
                     /translation="MERLIWGIAAVIGGFTLTTLTRGEKHQTKGLLLTDQELKSKLTP
                     EQYRVVRENGTEPAFANAYWDNHAEGIYVDVVSGKPLFSSKDKFDSGTGWPSFTRPID
                     AKEVVAKTDLAYGMVRTEVRSSGANCHLGHVFPDGPGPTGQRYCINSAALRFVPVDKL
                     ESEGYGEYRSLFNPKS"
     gene            2275..2862
                     /locus_tag="PK362_02940"
     CDS             2275..2862
                     /locus_tag="PK362_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC73829.1"
                     /translation="MGASETDDFDLIRRVAQDRDESAFSRIYEQYAPRVFGLACRMMN
                     RREEAEEVLQDVFFSLWEKAATYDAQRAPVGLWIMVMTRSRCLDRLRKRSLREGKENS
                     LDVDETGRGLLDALADPLPTALEDLASEERRREVQAALAALPDAQRAVLEASFLKGES
                     QQEIADRTGEPLGTVKTRMRLGLAKLAEALMDRKQ"
     gene            2859..3686
                     /locus_tag="PK362_02945"
     CDS             2859..3686
                     /locus_tag="PK362_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC73830.1"
                     /translation="MNHDELKDLIPAFALGTLSAEESAVLKAHLGEDCAECGRALREA
                     HWAVLALAQSAGDETPPPRVKRALMAKISPAARWSWRPVLALAAGALIVVGLFRGFST
                     SQGRLEVLGSSGEITAEGRSVSTGATINLGETIEATRGKTDFRLASRVVFRLNEGARV
                     VVLRRDGRFVVRLSRGAILSVVKHGTPYAVESPVAVAAVRGTAFYMSVDSPQKTSVCV
                     CEGRVRVHALDKTEEVVATHHTAASWRRDGDAAVKGPPGALDHSDRDVETLRGFLPQ"
     gene            3772..>4093
                     /locus_tag="PK362_02950"
     CDS             3772..>4093
                     /locus_tag="PK362_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNC73831.1"
                     /translation="MDKAKTFLESLPSRLDFLPGLLSRITLGFIFVQSGWGKLHHLDK
                     VTEFFRALGIPAPGLQAPFVATVEFVAGGLILLGLFTRLAALPLIGTMVVAILTAKRP
                     DIHEL"
BASE COUNT          793 a         1264 c         1209 g          827 t
ORIGIN      
        1 acgccggctt cccgatggcc gttgcccgtg tttttcactc cgccgaaggg caagtggact
       61 tcggccccga tggtgggggc gttgatgtag gcgatgcccg tttccatgtc ccgcacggcg
      121 cgcatggccc gtcgaatgtc ccgagtgtag agggaacccg agagcccgta ttccgtgtcg
      181 ttcaagacgg cgatggattc ctcaaaggaa tgaaccggaa cgatcgtcgt caccggaccg
      241 aaaatctcct cccgggcgat tcgcatggtg gggttcccga gaaaaaccgt cggctcgtgg
      301 taatgccccc cggcgtatcg gccggtggtc aaaacccgac cgccggtgag aaggacggcg
      361 cgatcgatct tttgtccgat tttaacgtag ttctgaacgc tttccagctg tcgcgcgctg
      421 atgagcggcc ccatttgaac gccgtcttcc aatccgttgc cgattttgat tttcttcgac
      481 gccgccacaa agcggtccgt aaaagtcttg agcaccgctt tgtgaaccag gatacggctg
      541 gtggccgtac agcgttgacc ggaagttccg aaggcccccc acagggcgcc ttccagggcc
      601 aaatccaaat cggcgtcgtc catgacgatc tgggcatttt ttccgcccaa ttccagggaa
      661 catttcttgt acaaacgtcc cgccacctcc cccacgttgg aacccacgga agacgagccc
      721 gtaaaggaaa ccaggcgcac gtccgggtgg gcgatcagcc tctggccgac ctccgagccg
      781 ttgccgaaaa cgatgttcac gacccccggg ggaacgccgg cttcctccag agtttgaacg
      841 aaatttcgag ccgaatccgg ggtgtaggag gcgggtttga ggaccacggt gttgccgcag
      901 agcagggccg ggtagatttt ccaggagggg atcgccatcg gaaaattcca gggcgatatc
      961 aaggcgcaga cgcccaccgg ctgtcgaagc gacatggcga atttgtccgg aagttccgag
     1021 gtggtcgttt gtccgaaaag acgacggccc tctcccgcgt aataaaaggc ggtgtcgatg
     1081 gcttcctgga cgtcgccccg ggtttccgtc aacaccttcc ccatttcgcg ggtcatggcc
     1141 cgggcgaatt tctcctttcg ctcctccaga aggcgcatgg atttaagcag aatatcgcca
     1201 cgccggggga cgggcatttt gcgccaggcc ccgtaggcct tcttggcggc ggccacggcg
     1261 tcgtccacgt cggacggaga agcggccgga taaacgttca ggcgccgccc cgtggcggga
     1321 tcaaaattct cgaaggcgcc cccgccgggc ccggcgccgc gccacacgcc gccgatcaaa
     1381 aaggcttttt ccgaagacac gaggtttttt ttggaaaggg tcatggattg tcctgggagt
     1441 ggaatagaaa ccaacggggc ggcagggagg cggaaacccc cgccgggttg gaccgcgaga
     1501 ggaacgtcat ggccggatta taaatattcc ggcccccaaa gcaaacgggc ggaggccctt
     1561 ggcctccgcc cgtcgcccga ccggtcgatt ccggctacga tttcggatta aacaaggacc
     1621 ggtattcccc ataaccttcc gattccaact tatccaccgg cacgaaccgc agggccgcgg
     1681 aattgatgca ataacgctgg cccgtcgggc ccggtccgtc cgggaacacg tgtcccaagt
     1741 ggcaattggc tccggaggag cgaacctcgg tgcgcaccat cccgtaggcc aaatccgtct
     1801 tagccaccac ttccttggcg tcgatgggcc gggtgaagct cggccaaccc gttcccgaat
     1861 cgaacttgtc tttggagctg aagagcggct tcccggaaac cacgtccacg tagatccctt
     1921 cggcgtggtt gtcccaataa gcgttggcga aggccggttc ggttccgttt tcgcgcacca
     1981 cccggtattg ctccggcgtc agtttcgatt tcagttcttg atcggtcaag aggagtcctt
     2041 tcgtttgatg tttttcgccc cgggtcaacg ttgtcaaggt gaatcctccg atcaccgcgg
     2101 cgatccccca aatcaacctt tccatcgttt tctcctttta tccctttgac gctggccagg
     2161 gtgtttttgt taatataacc catctagtca tgtatacggc caaaaccctc cggtggatct
     2221 ccccctttcc aacacatcta attccccatt ccccgcgtat aaccattaag gaccatgggc
     2281 gcctctgaaa cggacgattt cgatttaatc cgccgtgtgg cccaggaccg ggacgaatcc
     2341 gctttttccc ggatatacga acaatacgct ccccgggttt tcggtttggc ctgccgaatg
     2401 atgaaccgcc gggaggaagc cgaggaagtt ctgcaggacg tttttttctc tctctgggaa
     2461 aaggccgcga cctacgatgc ccagcgagcc ccggtgggtc tctggatcat ggtcatgacc
     2521 cgaagccggt gcctggatcg cctgcgaaag cgatccctgc gggagggaaa ggaaaacagc
     2581 ctggacgtcg atgagacggg gcggggattg ttggacgctc tggccgatcc cctgcccacc
     2641 gccctggagg acctggcctc ggaagagcgc cgacgggagg ttcaagccgc tctggcggcc
     2701 ctgcccgacg cccaacgggc ggtgctggag gcttcgtttc ttaaaggaga gtcccagcag
     2761 gaaatagcgg accgaaccgg cgaaccgctt ggaacggtca agactcgaat gcgtctcggc
     2821 ctggcgaaat tggccgaagc gttaatggac cgaaaacaat gaaccacgac gaattaaaag
     2881 atctgatccc ggcctttgcc ctgggaaccc tgtcggccga agaatcggcc gtccttaaag
     2941 cccatttggg cgaagactgt gccgaatgcg gccgcgctct tcgggaggcc cattgggccg
     3001 tgttggcctt ggctcaatcg gccggcgacg agacgccgcc tccgcgcgtt aaacgcgccc
     3061 tgatggcgaa aatctccccc gctgcccgtt ggtcctggcg ccccgtcctc gccctggcgg
     3121 cgggggccct gatcgttgtg ggcctttttc ggggattttc aacttcccaa ggccgcctgg
     3181 aagtcctggg gagctcggga gaaatcacgg cggaaggacg atcggtgtcg acgggagcaa
     3241 cgataaatct tggggagacc attgaagcca ctcggggtaa aacggatttt cgattggcct
     3301 cccgcgtcgt tttccgattg aacgagggcg cccgagtggt cgtcctgcgg cgggacgggc
     3361 gtttcgtggt ccgcctctcc cggggcgcca tcttaagcgt cgtcaaacac ggaaccccct
     3421 acgcggtgga aagccccgtc gccgtggccg ccgttcgggg aacggcattt tatatgagcg
     3481 tggattcgcc ccaaaaaact tccgtttgcg tgtgcgaagg gcgcgtgcgg gttcacgctc
     3541 tcgataaaac cgaggaagtc gtcgccaccc atcacacggc cgcgtcctgg cggcgcgacg
     3601 gcgacgccgc ggtcaaaggc ccccccggcg ccttggacca ttccgaccgg gacgtcgaaa
     3661 ccctccgggg gtttctgccc caataatcca tagatccaaa accgaagttc gcccgtaaca
     3721 taagggacgg ttccaaagga atcggacatt cgaactcgga gaaggtggat catggacaag
     3781 gcaaaaacat ttctcgaatc gcttccctcc cggctggatt ttctccccgg cctgctcagc
     3841 cgcatcaccc tcggatttat tttcgttcaa tcgggctggg gcaaacttca ccacctggat
     3901 aaagtcaccg agtttttccg cgccctcggc ataccggccc ccggcctcca ggcccccttc
     3961 gtggcgacgg ttgaattcgt ggcggggggg cttattctgc tgggcctttt cacccgactc
     4021 gcggctcttc ccctcatcgg aaccatggtc gtcgccattc tgaccgccaa gcggcccgac
     4081 atccatgaac tgt
//
LOCUS       DAOQLS010000108         4091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10858_length_4091_cov_4.061943, whole genome shotgun sequence.
ACCESSION   DAOQLS010000108 DAOQLS010000000
VERSION     DAOQLS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_4091_cov_4.061943"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK362_02955"
     CDS             <1..531
                     /locus_tag="PK362_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC73832.1"
                     /translation="LDPAADTTDLRRRVSMIVDENTPLPMSIADNLRLGLRFTHGAGA
                     WEERIESSLRASFLWDEVKDRLGSSALDLSGGQQQRLCLARSLMLQPDVLLLDEPCSG
                     LDPISTAKIEEALQGLKKSMSVVLVTNNVKQASRTSDRTAFLLMGDLIEVGDTGQVFT
                     SPAQERTADYVSGRFG"
     gene            536..1330
                     /gene="pstB"
                     /locus_tag="PK362_02960"
     CDS             536..1330
                     /gene="pstB"
                     /locus_tag="PK362_02960"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC73833.1"
                     /translation="MEQTPAAPAAPSEPGPILEARELNLYYGAFHALKNVSVAIPDKS
                     ITAFIGPSGCGKSTLLRTFNRMNDLIHGVRVTGQVLLHGRDVYAPKADLIALRKRVGM
                     VFQRPNPFPISAYENVAYGPRVHHLFRGKELDAVVEKSLRSTGLWDEIKDRLDTPALA
                     LSPEQQQRLCISRLLAVAPEVLLMDEPCSALDPIATGRIEEMIQELKKAYSIVIVTHN
                     MQQAARVSSQTAYFLLGELVEFGPTARIFTSPRDPRTEQYITGRFG"
     gene            1341..2012
                     /gene="phoU"
                     /locus_tag="PK362_02965"
     CDS             1341..2012
                     /gene="phoU"
                     /locus_tag="PK362_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNC73834.1"
                     /translation="MERHFDEELNQLKERLLRMGAIAEEMIQKAVKALRERSEKLTQE
                     VFESEKMVNQMHVEIDDRCLKLIALHQPMAADLRLITAAMKINSDLERIADQAVNAGQ
                     TCYYHLFKESPVPQISMITQMAEISQKMLRDSLDAFSRRDVALAEKVLKQDEEEDRLK
                     AQALTSLIDLLKKDPSHSSQYVDLILLSRNMERIGDHATNVAEDVIFMVQGKDIRHHL
                     DHRDE"
     gene            2194..3399
                     /locus_tag="PK362_02970"
     CDS             2194..3399
                     /locus_tag="PK362_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HNC73835.1"
                     /translation="MTTLEDSGPGSLREALMDPRPRVVVFRVGGTLRLREDLFLRHPF
                     ITVAGQTAPGDGILIRDFGLTILTHDVLIQHLRIRPGAERQKNPENNDGLGLLGEAYG
                     GDVHDVVLDHLSVSWAEDENVSVLGGLRDITVSHCLIAEGLNRSRHAKGTHSAGLLID
                     DTATATVYRCLLAHNGFRNPVFLNNYRKANVLAEMVETAVYDWGDVGTEITNYAADKT
                     AGLRLNLVGNVYRAGPSLNAGFPFPIGIGREGNPRLYLRDNVDGTGARDDALMSGIGF
                     RLNREDLRLDQPFDTPAVSAATDSSDVWGEALAAAGATRPRRDAVDARIVEEARKGTG
                     RIIDSPDQGGGYPTLRDAPPPRDSDHDGMPDDWETSRGLNPRFPEDRNDDRNGDGYTN
                     LEEYLFSRL"
     gene            complement(3485..>4091)
                     /locus_tag="PK362_02975"
     CDS             complement(3485..>4091)
                     /locus_tag="PK362_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73836.1"
                     /translation="PATPLIASGHIHTRQAFQLDHATIVHCGSTERTSYNERDETKGY
                     VLWELERSARWRHVDLPARPMAWITHPDQIDEAEAGALAAAPAGASVERSLYKSKAQY
                     AESMNSVDAVSGVAGGTLKAAELKKDDLPAELREKDEKELEEALRAKGAEREKIQKQL
                     NALGSKRQSFLSEKSAGNKDSLDQAVLNAVRSQASKFGFTF"
BASE COUNT          742 a         1353 c         1248 g          748 t
ORIGIN      
        1 ttagacccgg ccgccgacac caccgacctt cggcggcggg tcagcatgat cgtcgacgag
       61 aacacgccgc tccccatgag catcgccgac aatttgcgcc tgggcctccg gttcacccac
      121 ggggcgggcg cctgggagga acgcatcgaa tccagccttc gagcctcttt tctctgggac
      181 gaggtgaagg accgcctggg atcctcggcc ctggatcttt ccggcgggca gcaacagcgc
      241 ctttgcctgg cccgcagcct gatgctccaa cccgacgtgt tgctcctgga cgagccctgc
      301 tccggattgg acccgatttc cacggccaaa atcgaggagg cgctccaggg tttgaaaaaa
      361 tccatgtccg tggtgctggt gaccaacaac gtcaagcagg cctcccgcac ctcggaccgc
      421 accgcctttt tgttgatggg cgatttgatc gaagtgggcg acacgggaca ggtgttcacc
      481 agccccgccc aggaaaggac cgccgactat gtctccggac gttttggata ggcctttgga
      541 gcaaaccccc gccgccccgg cggcgccctc ggaacccggc cccattttgg aggcccggga
      601 gctcaatctg tattacggcg cctttcacgc cctgaaaaac gtctccgtcg ccattcccga
      661 caagtccatc accgccttca tcggcccctc gggatgcggc aagtccacgc tcctgcgcac
      721 cttcaaccgc atgaacgatc tgatccacgg ggtgcgggtc acggggcagg ttctcttgca
      781 cggccgggac gtctacgccc ccaaggccga tttgatcgcc ctgcgaaagc gcgtgggcat
      841 ggtgtttcag cggcccaacc cctttcccat ttcggcctac gaaaacgtgg cctacggtcc
      901 ccgggtgcac catttgttcc ggggaaaaga gctcgacgcc gtggtggaaa agtccctccg
      961 ctccacgggc ctttgggacg aaatcaaaga ccgcctggac acgccggccc tggccctttc
     1021 gccggaacaa cagcagcggc tttgcatttc ccgccttctg gcggtggcgc cggaagttct
     1081 tttgatggac gaaccctgtt cggccctgga ccccatcgcc accgggcgca tcgaggaaat
     1141 gatccaggaa ttgaaaaagg cctattccat cgtcatcgtg acgcacaaca tgcagcaggc
     1201 ggcccgggtg tcgtcccaga cggcgtactt tttgttgggg gagttggtgg agttcggtcc
     1261 gacggcgcgc attttcacat cgccccggga tccgcggacg gaacaataca tcacggggcg
     1321 cttcggataa ggagatttcc atggaacgac atttcgacga agaattgaac caattgaagg
     1381 aacggctcct ccgcatgggg gccatcgccg aggaaatgat tcagaaggcg gtcaaagccc
     1441 ttcgggaacg gagcgagaag ctcacccagg aggtctttga gtccgaaaag atggtcaacc
     1501 agatgcacgt ggagatcgac gaccggtgcc tgaagctcat cgccctccat cagcccatgg
     1561 cggcggacct gcggctgatc acggcggcca tgaagatcaa ttccgacctg gaacggatcg
     1621 ccgaccaggc ggtcaacgcc ggtcagacct gctattacca cttgttcaag gaatcgcccg
     1681 ttccccagat cagcatgatc acccagatgg cggaaatcag ccagaagatg ctccgcgaca
     1741 gcctcgacgc cttttcccgg cgggacgtgg ccctggccga gaaagttctc aaacaggacg
     1801 aggaggagga ccggctcaaa gcccaggccc tgacgagcct catcgacctt ctcaagaaag
     1861 accccagcca ctcctcccaa tacgtggact tgattttgct gtcccggaac atggaacgca
     1921 tcggcgacca cgccaccaac gtggccgagg acgtgatctt catggtccag ggcaaggaca
     1981 tccgccacca cctggaccac cgggacgaat aacctgggac gtcccgggca ccggtcccgc
     2041 cgcggtttcc tcgcggccct ctccgttatc ggatccgttc ttccgcccct ttgggcggga
     2101 aactccccgg cgccccgccc tcttcccgtt ttcccgggcg ccgtcgggtt cggtacgacc
     2161 acgccggccg ggcgcggggg cgtcgtcctg gaagtgacga ccctggagga cagcggcccc
     2221 ggttccctgc gggaagccct gatggacccc cggccccgcg tcgtcgtttt tcgtgtgggc
     2281 gggaccctcc gccttcggga agacctcttc cttcgacatc cctttatcac ggtggcgggg
     2341 caaacagccc ccggcgacgg gatcctgatc cgggatttcg gcttgacgat tctgacccac
     2401 gatgtgttga tccaacacct gcgcatccga cccggggccg agcgccaaaa aaatccggaa
     2461 aacaacgacg gcctggggct tctgggggaa gcgtacggcg gggacgtcca cgacgtcgtc
     2521 ttggaccacc tgtccgtgag ctgggccgag gacgagaacg tcagcgtcct gggcggtctt
     2581 cgggacatca ccgtttccca ctgcctcatc gccgagggat tgaaccgaag ccgccacgcc
     2641 aagggcaccc acagcgccgg cctgttgatc gacgacacgg ccacggccac ggtttatcgc
     2701 tgccttctgg cccacaacgg atttcgaaat ccggttttct taaacaacta ccggaaagcg
     2761 aacgtcctgg ccgagatggt ggagacggcc gtttacgact ggggcgacgt gggcaccgaa
     2821 atcaccaact acgcggccga caaaacagcg ggccttcgtt tgaatctggt cgggaacgtc
     2881 tatcgagccg ggccgtcgct caacgcgggg tttccctttc ccatcggcat cggacgggag
     2941 gggaatcccc ggctctacct tcgggacaac gtggacggca cgggggcccg ggacgacgcc
     3001 ttgatgtccg gcatcggatt tcggttgaat cgggaagacc ttcggctcga ccaacccttc
     3061 gacacccccg ccgtgtcggc ggcgacggac tcctccgacg tttggggcga agcgctcgcc
     3121 gcggccggcg ccacccggcc ccgccgggac gccgtggacg cgcgaattgt ggaagaggct
     3181 cgaaaaggca cgggccggat catcgactct ccggaccagg ggggcgggta cccgactctt
     3241 cgggacgccc cgccgccgcg ggactccgac cacgacggga tgcccgacga ctgggaaaca
     3301 agccgggggc tcaatccccg ttttcccgag gatcgaaacg acgatcgaaa cggggacggt
     3361 tacacgaatt tggaagaata cctgttctcc cggctgtaga attcccccgt tatcggtttt
     3421 cctctaaaaa aacgcggccc ccgcttttca acgggggccg cgcgccttgc ctcgtggtgt
     3481 ccgattagaa ggtgaacccg aatttggacg cctgggaccg gaccgcgttc aggaccgcct
     3541 ggtccaaact gtccttgttg cccgcggatt tctccgacag gaacgattgc cgcttggacc
     3601 ccagggcgtt caattgtttt tggatttttt cccgctcggc ccctttcgcc cgcagggcct
     3661 cctccaattc cttttcgtcc ttttccctaa gctccgccgg gagatcgtct tttttcaatt
     3721 ccgccgcctt gagggtgccg ccggccaccc cgcttaccgc gtccacgctg ttcatggact
     3781 cggcgtattg ggctttggat ttgtaaaggg aacgctccac ggacgccccc gccggggccg
     3841 ccgccagggc gcccgcctcg gcctcgtcga tctggtccgg gtgggtgatc caggccatgg
     3901 ggcgcgccgg cagatcgacg tggcgccagc gcgcgctgcg ctccagctcc cacagcacgt
     3961 agcccttggt ctcgtcgcgc tcgttgtagc tcgtgcgctc ggtcgagccg cagtgcacga
     4021 tggtggcgtg gtcgagctgg aaggcctggc gcgtgtggat gtggccggag gcgatgagcg
     4081 gggtggcggg c
//
LOCUS       DAOQLS010000109         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_10987_length_4063_cov_4.065369, whole genome shotgun sequence.
ACCESSION   DAOQLS010000109 DAOQLS010000000
VERSION     DAOQLS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10987_length_4063_cov_4.065369"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK362_02980"
     CDS             <1..375
                     /locus_tag="PK362_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC73837.1"
                     /translation="DRLLGEVSGGQRQRAVLAQALCQSPDVLVLDEPTKGLDVMAERD
                     LLDLIAGLRSQRLTILLVSHSLQIPLNFTDRILLFAEGRVVDTTPGELTTTNRLEEIY
                     GAPFAHLEQEGMKWAMPRRRPQ"
     gene            372..1187
                     /locus_tag="PK362_02985"
     CDS             372..1187
                     /locus_tag="PK362_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNC73838.1"
                     /translation="MNVFAMDFMRTALAAALLIGLSLSILGVFLTLRRMAFSGLAVSQ
                     WAGLGTVIGVALGWHWGADALALGFVALGMYLLTRLSNGRRTRPDSWVASLYVLGAAG
                     AVLVLAKAPRGESETLSLFFGNVLALTGVEVKEALGLFLVTAATLGLGFYRWVWVSFD
                     PVSAEVAGVRVARWTFAFNLLFAIAMTLAIHIVGVLLAFAFLLLPATAGLTSGARLKT
                     VFVGAAGTAIVATLLGFVFSVRWDLPTGPLVAALLAGATLAARLTAWARGRAD"
     gene            complement(1194..2708)
                     /locus_tag="PK362_02990"
     CDS             complement(1194..2708)
                     /locus_tag="PK362_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73839.1"
                     /translation="MKDHEGLVRRHDKYQFEVKLGYDLTPHHRRDRYRVETFFFLPEN
                     LDVNESTYSKGQFYRDLLLYIRFRTPDFSLAALLDPAEERSPLQRVPAKLAAFLESPS
                     VRNARALDYELRLMGCVFKNALREQSREIEPLLEKPGGGADAREHLTRYRENARAVLV
                     RYRALRDRFHTPGVPAGLRATFAFADEYMSLLVEGRTHDLLQRVQNLNAPAFAETETG
                     LAALVIDEIQYRKSRGFPSIVAEDKDNETFVFRQSVLKKFITSALHLVVRTEEEGKGL
                     EQMAMAIGAGIAMVFATTIAFYYQKVYGTLSLGFFAVLVVSYMFKDRLKAVVQHYLQR
                     RLARHLFDQATNIHDPFNRIKIGVCREAVGFVPERRVDPHVLRLRDRDHITEIENTWR
                     AEKVLHYVKEITLFSRRFWKNHSRKEGITDIVRFNLRNFLLKMDEPTTDLYFLKEGRS
                     VLVRGTRVYHVNIVIKFVSDDFTRYERLRLVLNREGLKAVEPVGSDTVPGPSDQ"
     gene            2805..3533
                     /locus_tag="PK362_02995"
     CDS             2805..3533
                     /locus_tag="PK362_02995"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNC73840.1"
                     /translation="MEPPKNLTLDQAEEWQSRLAARVNPRADAAAFAPGRTVAVLRFD
                     PVERRGRVRARGAVVRWSTREVLERAAVEWKATDLFPFVPGLEGFREAPILRAVLDQF
                     KTAPDVLMVDGAGIAHRRRFGVACHLGVAVDKPSFGVSTLCDYGTWETPPPGLDGAHV
                     FVKDAGGEPLGVALRAVAYKDPLFVSPGHRAGVLPAIDVALALIEGRSAPDPFWVLAP
                     AEKKRRTSSRPRAAPTSKKRGKKT"
     gene            3530..3886
                     /locus_tag="PK362_03000"
     CDS             3530..3886
                     /locus_tag="PK362_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73841.1"
                     /translation="MMPTVVAMLLSAFVLPGSGQIYNGEKKKGMVFIGATLGLAFALF
                     VALTLAMADMMPPGGGLISVLEAKALVTRLRVEHGQMLAVFEWLFTGLWAVGVLDAYL
                     GARAKARREAGAAAPK"
     gene            3883..>4063
                     /locus_tag="PK362_03005"
     CDS             3883..>4063
                     /locus_tag="PK362_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNC73842.1"
                     /translation="MKRPRPVDVRAMRALDRRVVRDFGLPTAVLMENAGRAVAAEALR
                     MTRGPVAVLCGTGNNG"
BASE COUNT          637 a         1181 c         1448 g          797 t
ORIGIN      
        1 gaccgtttgc tgggcgaagt gtccggcgga cagcgccaac gggccgtgtt ggcccaggcg
       61 ctctgccagt ccccggacgt gttggtgttg gacgaaccca ccaaggggtt ggacgtcatg
      121 gccgagcggg acctgctgga tttgatcgcg ggtcttcgct cccaacggct gacgatcctg
      181 ctggtgagcc actccctgca gattccgctc aattttacag accgcatcct gctcttcgcc
      241 gagggccgag tggtggacac cacccccgga gagttgacca ccaccaaccg gttggaggag
      301 atttacggcg cgcccttcgc ccacctggaa caggagggca tgaaatgggc catgccccga
      361 cggagacccc aatgaacgtt tttgcgatgg acttcatgcg cacggccctg gccgccgcgc
      421 tcctgatcgg gttgtcgctt tcgattctcg gggtgttcct gaccctgcga cggatggcct
      481 tttcggggct cgcggtgtcc cagtgggcgg gtctgggcac ggtgatcggc gtggccctcg
      541 gttggcactg gggcgcggac gccctggctt tgggttttgt ggccctgggg atgtatcttt
      601 tgacccgcct ctccaacgga cggcgaacgc ggcccgattc ttgggtggct tcgttgtacg
      661 tcctgggcgc ggcgggagcc gtgttggtgc tcgccaaggc gccacggggg gaatcggaga
      721 ccttgagcct cttcttcggc aacgtgctgg ccctgacggg cgtcgaggtg aaggaagctc
      781 tcgggctatt cctggtgacc gcggcgactc tggggctcgg gttttatcgt tgggtctggg
      841 tgagtttcga tcccgtctcc gccgaagtgg cgggcgtgcg ggtggcccgg tggacgtttg
      901 ccttcaatct gttgttcgcc attgcgatga cgctcgcgat ccacatcgtg ggcgtcctgt
      961 tggccttcgc ctttctgctg ttgcccgcca cggcggggct cacctcgggg gcgcgtctaa
     1021 aaacggtctt cgtgggcgcc gccggaacgg cgattgtcgc cacgttgctg gggttcgttt
     1081 tttcggttcg gtgggattta ccaacggggc ccttggtggc cgcgcttttg gccggggcaa
     1141 cgctggccgc ccgattgacg gcctgggccc gggggcgggc ggattaaggt tttttattgg
     1201 tcggacgggc cggggacggt gtcgctgccg acgggttcga cggctttgag gccctcccgg
     1261 ttcagcacca agcggaggcg ctcgtaacgg gtgaaatcgt cggagacgaa tttgatcacg
     1321 atgttcacgt ggtacacccg ggtgccccgg acgaggacgg agcggccttc ctttagaaaa
     1381 tagaggtccg tggtcggttc gtccatcttc agcaggaaat tgcgcaggtt gaagcgcacg
     1441 atgtcggtga tgccttcctt ccgggagtgg ttcttccaaa aacgccggct gaagagcgtg
     1501 atctccttca cgtagtggag aaccttttcc gcccgccagg tgttttcgat ttccgtgatg
     1561 tggtcgcggt cccgcaggcg caggacgtgg ggatccacgc ggcgctccgg cacaaagccc
     1621 accgcttccc ggcagacccc gatcttgatc cggttgaagg ggtcgtggat gttggtggcc
     1681 tggtcgaaca ggtggcgggc caaacggcgc tgaagatagt gttggaccac ggccttgagc
     1741 cggtccttga acatgtagct gacgaccaga accgcgaaaa agcccaggga cagggtgccg
     1801 tagacttttt ggtagtagaa ggcgatggtg gtcgcgaaca ccatcgcgat gccggcccca
     1861 atggccatgg ccatctgttc aagccctttg ccttcctcct ccgtccggac caccagatgc
     1921 agggcgctgg tgatgaattt ctttagaacg ctttggcgga aaacaaaggt ctcgttgtcc
     1981 ttgtcctccg cgacgatgga ggggaacccc cgggatttcc ggtattggat ttcatcgatg
     2041 acgagggcgg ccaggccggt ttcggtttcg gcgaaggcgg gggcgttcag gttctgaacc
     2101 cgttgcagca agtcgtgggt gcggccttcg accaacaggc tcatgtattc gtcggcaaag
     2161 gcgaaggtcg cccgaagccc ggccggaacg cccggcgtgt ggaaacgatc ccgcagggcc
     2221 cggtaacgga ccaggaccgc ccgggcgttt tcccggtagc gggtcaagtg ttcccgggcg
     2281 tcggcccctc cgccgggttt ttccaacaac ggttcgatct cccgggattg ctcccgcaga
     2341 gcgtttttaa agacgcagcc catgaggcga agttcgtaat ccagggcccg ggcgtttcgg
     2401 accgaggggg attccaagaa ggcggcgagt ttcgccggga cgcgctgaag gggggaccgc
     2461 tcctcggccg ggtccaacag ggcggccagg gaaaagtccg gcgtgcggaa acggatgtaa
     2521 agcaacaggt cccggtagaa ctggcccttg gaataggtgg attcattgac gtcgaggttt
     2581 tccggaagga aaaaaaaggt ttcaacgcgg taacggtccc gccgatggtg gggggtgagg
     2641 tcgtagccga gtttgacttc aaattggtat ttgtcgtggc gccggacgag gccttcgtgg
     2701 tccttcatga cttaattgta ggcctcggac gggcccgacg caagggcgcg gcccccgggg
     2761 ggcgcaattg tctccggggg ggaaaaaagc gacaaggacc cttcatggaa ccgccgaaaa
     2821 acctcacgct ggatcaggcg gaggagtggc aaagccgact cgccgcccgg gtgaacccgc
     2881 gggcggacgc ggccgccttc gccccgggac gcacggtggc ggtgttgcgg ttcgatcccg
     2941 tggagcggcg ggggcgggtt cgcgcccggg gggcggtggt ccgctggtcc acccgggagg
     3001 tgttggaacg ggccgcggtg gaatggaaag cgaccgatct cttccccttc gtgccgggac
     3061 tcgagggatt tcgggaagcg ccgattttgc gggcggtcct ggatcaattc aaaacagcgc
     3121 ccgacgtcct gatggtggac ggggcgggca tcgcccaccg gcggcggttc ggggtggcct
     3181 gtcacctggg cgtggccgtg gacaaaccca gtttcggcgt gtcgacgctc tgcgattacg
     3241 gcacctggga aacgccgcct cccgggttgg acggggccca cgtgttcgtc aaagacgcgg
     3301 ggggggagcc cctgggcgtg gccctgcggg ccgtggccta caaagaccct ttgtttgttt
     3361 cgcccggcca ccgcgccggc gtgttgccgg ccatcgacgt cgcgttggcg ttgatcgaag
     3421 gccgttcggc gccggacccc ttctgggttc tggctccggc ggagaaaaaa cgtcggacgt
     3481 cgtcccggcc ccgggccgcg cccacgtcca agaaaagagg gaaaaagaca tgatgcccac
     3541 cgtcgtcgcc atgctgttgt ccgccttcgt tcttcccggg tccggccaga tttacaacgg
     3601 ggaaaagaaa aaaggaatgg ttttcatcgg ggccaccctg gggctggcct tcgccttgtt
     3661 cgtggccttg accctggcga tggccgatat gatgccgccg gggggcgggt tgatctcggt
     3721 gttggaggcc aaggccctcg tgacgcgcct ccgagtggaa cacggccaaa tgctggcggt
     3781 gtttgagtgg ctcttcacgg gattgtgggc ggtgggcgtg ttggacgctt acctgggcgc
     3841 ccgggccaag gcccgacgcg aagcgggggc cgccgcgccc aagtgaaacg cccccggccc
     3901 gtcgacgtcc gcgccatgcg ggcgctggac cgccgcgtgg ttcgggattt cggcctgccg
     3961 acggccgtgt tgatggagaa cgccggacgg gcggtggcgg cggaagccct tcgaatgacc
     4021 cggggccccg tggccgtgct gtgcgggacg gggaacaacg gcg
//
LOCUS       DAOQLS010000110         4025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11146_length_4025_cov_3.840554, whole genome shotgun sequence.
ACCESSION   DAOQLS010000110 DAOQLS010000000
VERSION     DAOQLS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11146_length_4025_cov_3.840554"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PK362_03010"
     CDS             <1..479
                     /locus_tag="PK362_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73843.1"
                     /translation="RRGDRAGAWIEAHRTLELEPAFGEAALLLGRLLWAEGDPRGADR
                     WLTGWWARWGTVSAPSGTGSAYREALLRRNSDAFLLERARARLSSGRYREALSLLSDL
                     PMDDPDRLLLESGCWYYLGDLIRAESLLRRVQRLAPGRRETGVLLEKVRQRRGGVR"
     gene            476..1204
                     /locus_tag="PK362_03015"
     CDS             476..1204
                     /locus_tag="PK362_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC73844.1"
                     /translation="MIHILLAAYNEEAALGDVLNGIARALADGDYKVWVVDDGSRDGT
                     SRVADEWRKKIPLIVLQHAVNRGLGAALQTGLDALRGALRETDVLVTLDADNTHPPAL
                     IPRLAAPIEEGTADIAIASRFVRGAVVAGVPFFRRLTGCAASWLFRIAVPLRGVRDYT
                     CGFRAYRGGLLRKGFAAPGRMITENGFACQAEWLIKLGRLAPRVVELPLLLRYDRKPT
                     PSKMPVLRTIGATLALLRRLRKLK"
     gene            1201..2172
                     /locus_tag="PK362_03020"
     CDS             1201..2172
                     /locus_tag="PK362_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC73845.1"
                     /translation="MKSPSRRFLIGLLLSAVGVWFAFRGARWGDVGRAVSHLSNPLAL
                     LLIPLAGLVEYALRTERWRLMTGISPTGRFALFPIVAGSFFINNVLPFRAGEAARVYW
                     THREKGRPLGEVVGALALDRAADVAALALLTLILVAVAPQTGLLSGRSALILLCAAVS
                     GAGLLLILGRDSFSTIRKRWLPESAQRFLLGLSTAATALGRPGVLTRTILLSAGVWAL
                     NVALDRAIADLFGLELSWAESGWFLVALAAGVALPSSPGYIGTFEAAGVGALVLLGRD
                     PVVSLSFVVVLHAGQIFGSAIWGGPGLWRRGRPGAGAAPANVIKSAP"
     gene            2169..3641
                     /locus_tag="PK362_03025"
     CDS             2169..3641
                     /locus_tag="PK362_03025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC73846.1"
                     /translation="MTRRKRLVAYGILGAVALISRAVGWGWGFPLKFGHIDESVVLFY
                     SLRIVDGAWAPGFFDYPGFFFYVLAGVLRATQTLGHWAGWVPSMGELLSGYLNGDSRF
                     FLLTARGLSMVFGLATVALTADMGRRRAGESVGWAAGVLLAVNPLHVLHCHYGTVDVA
                     AAFLTLLALDRIDVFWAHPTRRNGLGAAVAVGLGAATKYYPGILWLLVVAEPFWRRRP
                     RAAAWAAVLTVAALGAYAVGSPETLVSIREFARRFGHLFPKIVGAPGTAIPLVSTVRG
                     LSEGLGPLVLGGALVGVVRVLRGGPPGKVIVAGAGLLLGFIGFWRGQAPHYALALYPV
                     AALLAVQGFQWTGRHHRWCPAVLWGMSILLCLGGDARDWAMLRARDSRLEAGDWVRAH
                     VAPGAKILRSAHTPEFGARDGYSVKVDFTNQSFSDWASGGPIPDSLRGYDLLIYATYE
                     KADDAVLKVFMNHFPLRQSFARRPPRFPHHPRVFIFDGHG"
     gene            3634..>4025
                     /locus_tag="PK362_03030"
     CDS             3634..>4025
                     /locus_tag="PK362_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC73847.1"
                     /translation="MADRKTKIIAVLPAYNAEKTLEKTLADIPRADIDEILLVDDAST
                     DNTVALAERLGLPVVRHPSNRGYGGNQKTCYREALRRGADIVVMIHPDYQYDARLTPL
                     LTGFIERGVCDVMFGSRIRTRAEALRGG"
BASE COUNT          535 a         1207 c         1403 g          880 t
ORIGIN      
        1 aacgtcgggg cgaccgggcc ggagcgtgga tcgaagcgca ccggaccctc gaattggaac
       61 cggcttttgg agaagccgcc ctcctgttgg gacgccttct ttgggcggag ggcgatcccc
      121 ggggggccga tcgatggttg accggttggt gggcccgttg gggaacggtg tccgcgccgt
      181 ccgggaccgg gtccgcgtac cgggaggccc tgctccgtcg gaattccgac gcttttcttc
      241 tcgaacgggc gcgggcccgt ctttcgtcgg ggcgttaccg ggaggctttg tcccttctgt
      301 ccgatctccc gatggacgat cccgatcgct tgcttctgga gtcggggtgt tggtattacc
      361 tgggggattt aatccgcgcc gaatcgctgc tacggcgcgt tcaacgtctc gcgccggggc
      421 gccgggaaac cggcgtcctt ttggaaaaag tgcggcaacg gcgggggggt gttcgatgat
      481 tcatattctg ctggccgcct acaacgagga ggcggcgctg ggcgacgtgc tgaacggcat
      541 cgcccgcgcc ttggcggacg gggactacaa ggtttgggtg gtggacgacg gctcccggga
      601 cggaacctcc cgcgtggcgg acgagtggcg aaaaaaaatt ccgctcatcg tccttcaaca
      661 cgcggtgaac cggggcctcg gcgccgcctt gcagaccgga ttggacgccc ttcggggcgc
      721 ccttcgggag acggatgttt tggtcacctt ggacgccgac aacacccacc ccccggccct
      781 cattccccgc ctggccgcgc ccatcgaaga agggacggcg gacatcgcca tcgcttcccg
      841 attcgtgcgg ggggcggtgg tcgcgggtgt tccgtttttc cgtcgattaa cggggtgcgc
      901 cgcttcctgg ctcttccgaa tcgccgttcc cctgcgcggg gtccgggatt acacctgcgg
      961 attccgcgcc tatcgggggg gcttgctgcg gaaggggttt gcggccccgg gacgcatgat
     1021 cacggaaaac ggattcgctt gccaggcgga atggctgatt aaattgggcc gcctcgcccc
     1081 gcgcgttgtg gagcttcccc ttcttctccg ctacgaccgg aaacccaccc ccagtaaaat
     1141 gcccgtcctg cggaccatcg gggccacgtt ggcgctcctg cgtcggcttc gaaaactgaa
     1201 gtgaaaagcc cctcccggcg attcttgatc gggcttctgc tgagcgcggt cggcgtgtgg
     1261 ttcgcttttc gcggcgcgcg gtggggcgac gtcggtcgcg ccgtgtccca cctgtcgaac
     1321 ccgttggccc ttctcctcat cccgctggcg ggtttggtcg aatacgccct gcgaacggaa
     1381 cgatggcgcc tgatgacagg gatttcaccg acgggtcgat tcgccctttt tcccattgtg
     1441 gcgggttctt ttttcatcaa caacgtgctg ccctttcggg cgggagaggc ggcccgggtg
     1501 tattggacgc accgggaaaa gggccgcccc ctgggggagg tggtgggggc cctggcgctg
     1561 gatcgcgcgg cggacgtggc cgcgctggcg ctgttgaccc tgattttggt ggccgtcgcc
     1621 cctcaaaccg gcctattgtc cggccgatcc gcgttgattt tactctgtgc cgcggtgagc
     1681 ggggcgggcc tcctgctgat tctgggtcgg gattcttttt cgacgattcg aaagcgatgg
     1741 ttgcccgaat ccgctcaacg attccttttg gggctgtcca ccgccgccac cgccctgggg
     1801 cggcccgggg tcctgacgag gacgattctc ctgtcggccg gcgtctgggc cctgaacgtg
     1861 gccctcgatc gggccatcgc cgaccttttt gggttggaac tttcctgggc cgagagcggt
     1921 tggttcctgg tggccctggc ggccggggtg gccttgccct cctcgcccgg ctacatcgga
     1981 acttttgagg ccgccggcgt gggcgctctc gtcctcctgg gacgggaccc cgtcgtgtcg
     2041 ctttccttcg tcgtcgtact ccacgcgggg cagattttcg gatcggccat ttggggggga
     2101 cccggccttt ggcggcgggg tcggccgggc gctggggcgg ccccggcgaa tgtgataaaa
     2161 tccgccccat gacgcggcga aaacgtctgg tcgcctacgg gattctgggc gccgtcgcgt
     2221 tgatctctcg ggcggtggga tgggggtggg gatttccgct caaattcggc cacattgacg
     2281 aatcggtcgt tcttttttat tccttgcgga ttgtggatgg ggcctgggct cccggttttt
     2341 ttgattaccc gggatttttc ttttatgttt tggccggcgt tctgcgggcg acccaaactc
     2401 tggggcattg ggcgggatgg gtcccctcca tgggggaact gctctccggg tatctgaacg
     2461 gtgattcccg ttttttcctt ctcacggccc ggggattgtc gatggttttc ggattggcca
     2521 cggtggcgtt gaccgccgat atgggacgcc ggcgggcggg ggagtccgtc gggtgggccg
     2581 cgggcgttct actggcggtg aatccccttc acgttctgca ctgccattac ggcaccgtgg
     2641 acgtcgcggc ggcgttcctg acgctgttgg ccctggaccg gatcgatgtt ttctgggcgc
     2701 acccgacccg acgaaacggt ttgggcgcgg cggtggcggt gggcttgggc gcggccacga
     2761 agtattaccc ggggattcta tggctgttgg tggtggcgga gcctttttgg cggcgtcgac
     2821 cgcgggccgc cgcctgggcg gcggttttga cggtggcggc cttgggcgcc tacgcggtcg
     2881 ggtctccgga aacgctggtt tccattcggg aattcgcgcg gcggttcggg cacttgttcc
     2941 ccaaaattgt gggggcgccg ggaacggcga tccccttggt ctcgacggtc cggggcctgt
     3001 ccgaggggct cggccccttg gttctggggg gcgctttggt gggagtggtg cgggttctgc
     3061 ggggaggccc gcccgggaaa gtcatcgtcg cgggggccgg cctgcttctg ggttttatcg
     3121 ggttttggcg cggtcaagcg ccccattacg ccttggcgct ctatcccgtg gcggcgttgt
     3181 tggccgttca agggtttcaa tggacgggtc ggcaccatcg atggtgtccg gcggtgttgt
     3241 ggggaatgtc gattttgttg tgtctcggag gcgacgcccg tgattgggcg atgttgcgcg
     3301 ctcgggattc gcggttggag gcgggcgatt gggtgcgggc ccacgtcgcg cccggcgcca
     3361 aaattctccg atcggcccac acgccggaat ttggggcgcg ggacgggtat tcggtcaaag
     3421 tcgatttcac caatcaatcg tttagcgact gggcttcggg gggtccgatt cccgattccc
     3481 tgcgggggta cgatttgttg atctacgcaa cttacgaaaa ggcggacgac gctgtgctga
     3541 aggtctttat gaaccatttc cctttgcgcc aatcttttgc gcggcggccg ccccggttcc
     3601 cccaccatcc gcgcgttttt attttcgacg gtcatggctg atcgaaaaac aaaaatcatc
     3661 gcggtgttgc ccgcctacaa cgccgaaaag accctggaga aaaccctggc cgacattccg
     3721 cgggcggaca tcgacgagat ccttctcgtg gacgacgcct cgacggacaa cacggtcgcc
     3781 ttggccgagc ggttgggcct tcccgtcgtc cgccacccgt cgaatcgcgg ctacggggga
     3841 aatcaaaaaa cgtgttaccg ggaagccctc cggcgcgggg ccgacatcgt ggtgatgatc
     3901 cacccggatt accaatacga cgcgcggctc accccgcttc tcacgggatt catcgaacgc
     3961 ggcgtttgcg atgtgatgtt cggcagccgg atccgaaccc gcgccgaggc cctccgcggc
     4021 ggaat
//
LOCUS       DAOQLS010000111         4011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11206_length_4011_cov_4.168352, whole genome shotgun sequence.
ACCESSION   DAOQLS010000111 DAOQLS010000000
VERSION     DAOQLS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11206_length_4011_cov_4.168352"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PK362_03035"
     CDS             complement(<1..899)
                     /locus_tag="PK362_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC73848.1"
                     /translation="MTAVSPTRTSVAEIEETLARRLGRRHCVLTNRGTTALAAAFHAL
                     DRAPGTRALFPAAMCSIPVFAAGYAGWAPGFADVSLADGNFDLADVERALVESPGGVG
                     AVVPVHMFGKMDDVHALWDLCRRHGADLVEDVALSLGARRGETPAGGVGRISTLSFVR
                     KMLPLEMGGAVVTDEPALAARARAFVAALPPEDKRRRGEVAAAMKAFHNVTAYVAAGG
                     WARRTLLAPYAEEFRRLLLASVPDGDWADSIVLDELNKLDDVVQARRARAEVYETTLS
                     HPALSMMDRSGSSLFAFPVRLPG"
     gene            complement(896..1141)
                     /locus_tag="PK362_03040"
     CDS             complement(896..1141)
                     /locus_tag="PK362_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73849.1"
                     /translation="MSRDAESPVDVYAAQREELMSTLIGLSRRNEPLRVHEKNRLTPD
                     DIVRVLQKIRHGYGFSRTERTALTDAGFDLDRLFPDR"
     gene            complement(1173..2135)
                     /locus_tag="PK362_03045"
     CDS             complement(1173..2135)
                     /locus_tag="PK362_03045"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HNC73850.1"
                     /translation="MKKLAAAAAAVFPIWGYLLLKAELSGGAACWGWGTFLAALGVFS
                     FLWFLESFRRYRLLEDTPTAKIASAPQGYVEIDGLAENLPGKSLKSPLSLQPCVWFRY
                     SVQEKQGSGKNERWVTIDSGESATSFLVKDGTGEALVLPAGADIAPAVNRSWSGSTPN
                     PASEFVPTGSTFFSGFGRYRYHEELLAESFPVHVAGWFETIQPSRDTEKELNDKLRSL
                     KADPEKLKALADRDGDGQISGEEWDALRARWRGELIEANAHRPVEPAVHTMMIPSDHA
                     LPFIISGKGEDGAVSLYRWWSLAGAVGFLTAGAGCVFLLKKVLP"
     gene            complement(2132..2704)
                     /locus_tag="PK362_03050"
     CDS             complement(2132..2704)
                     /locus_tag="PK362_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNC73851.1"
                     /translation="MIVAFVVSAGLVLALVFGVGVYNGLVRLKNNVGKAWSNIDIVLK
                     QRHDELPKLIETCKQYMKFEQDTLEKVMKARAAVFSARETGNVAALGQAEGQLRGVLG
                     GLFAVAENYPDLKTNQTFLNLQTRVSSLENTISDRRELYNESVNLNNTRLEQFPDSLV
                     ARFGSFKAFEFLKFDAAETQDVNVKNLFSA"
     gene            complement(2772..3389)
                     /locus_tag="PK362_03055"
     CDS             complement(2772..3389)
                     /locus_tag="PK362_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNC73852.1"
                     /translation="MSGNNETPARSAGDALDLIGRMTVDPAAAFDDVAAHPRVGSGLL
                     VMTLAVAGRVLSDPLPWDDGAVALVRRFGATVAGSLVLWFLLAALGHGLSRLAGKKGR
                     FESFLSVAGWAGLALWGELPFRLLAAAAPDVALLASPPRLLFRCVAYGLWWWGLQRVY
                     GWSRGGALLLMLIPALAVGTLVFALGLAAALFFTGSAAFFHSLIH"
     gene            complement(3376..>4011)
                     /gene="sppA"
                     /locus_tag="PK362_03060"
     CDS             complement(3376..>4011)
                     /gene="sppA"
                     /locus_tag="PK362_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNC73853.1"
                     /translation="ASVQEIHGAVLALKGDGKKVVASLGDIAASGGFYVAAAADRIVA
                     NPGSLVGSIGVVFHLMNFEELTKKIGVQATVIKSGAMKDMGSPYRPLSAEERRVFEGL
                     VASAYGQFLEAVARGRKMPLDQLRPLADGRVFTGEQARAAGLVDALGSYDDALKEAAA
                     LAGIKSAHPKIIGPVRSWDRWLEAFGGLFEGPLGEWRRLLGVRGSLLYVWE"
BASE COUNT          779 a         1340 c         1260 g          632 t
ORIGIN      
        1 aaccccggca atcgcacggg aaaggcaaag agactggagc cgcttcggtc catcatggac
       61 agggccgggt ggctcaaagt cgtctcgtac acctcggccc gggcccggcg ggcctggacg
      121 acgtcgtcca gcttgttcaa ttcgtccagc acgatggaat ccgcccaatc gccgtcgggg
      181 accgacgcca ggagcagccg gcgaaactct tcggcgtagg gcgccagaag ggtccggcgc
      241 gcccaaccgc cggcggccac gtaggcggtg acgttgtgaa aggctttcat ggcggcggcc
      301 acttcgccgc gacgcctttt gtcctcgggc ggaagggccg cgacgaaagc ccgggcccgg
      361 gccgccaacg ccggctcgtc ggtcaccacc gccccgccca tttccagcgg aagcatctta
      421 cgaacaaagc tcaaggtgga aatccgaccg acgccccccg ccggggtctc gccccgccgg
      481 gcgcccaggg acagggccac gtcctccacg aggtccgcgc cgtgccgtcg gcacaggtcc
      541 cacaaggcgt ggacgtcgtc cattttgccg aacatgtgga cggggaccac ggcgccgacc
      601 ccgccggggg attccaccag cgcccgttcc acatcggcca aatcaaaatt tccgtccgct
      661 aaactcacgt ccgcgaaacc gggcgcccac ccggcgtacc cggccgcgaa cacggggatt
      721 gagcacatgg cggcgggaaa gagggcccgc gttcccgggg cccggtcgag ggcgtgaaaa
      781 gcggcggcca gggcggtcgt gccccggttg gtcaagacgc aatggcgccg gcccaggcgc
      841 cgggccaggg tttcctcgat ttccgcaacg gaggtccggg tcggggacac ggcggtcatc
      901 ggtcgggaaa caatcgatcc aaatcgaatc cggcgtcggt cagggccgtc cgctccgttc
      961 gggaaaaacc gtacccgtgg cggatctttt gcagaacccg gacaatgtcg tccggcgtca
     1021 aacggttctt ttcgtgcacg cgcaggggct cgttccggcg ggacagaccg atcaaggtgg
     1081 acatcaattc ttcccgctgg gcggcgtaaa catcgaccgg ggattcggcg tcgcggctca
     1141 tggacggcat tgtacgataa tccccccggg gttcacggta ggaccttctt aagcaggaaa
     1201 acgcaccccg cgccggcggt caaaaaaccc accgcccccg cgaggctcca ccaccggtaa
     1261 agggacacgg cgccgtcctc cccttttccc gaaataatga acggaagagc gtggtccgag
     1321 gggatcatca ttgtgtggac cgccggttcc accgggcgat gggcgttcgc ctcgatcaat
     1381 tcgccccgcc accgggcccg caacgcgtcc cattcctccc cgctgatctg gccgtccccg
     1441 tcccggtcgg ccagggcctt caatttttcc gggtcggcct tcaacgaacg gagtttatcg
     1501 tttaactcct tttccgtgtc ccgggagggc tggatggttt caaaccaccc ggccacgtgg
     1561 accggaaaac tttcggccag caattcctcg tggtaacggt aacggccgaa cccggaaaaa
     1621 aaggtcgacc cggtcggaac gaattccgag gccgggttcg gggtgctgcc ggaccaggac
     1681 cggttgacgg ccggggcgat gtcggccccc gcgggaagga cgagagcctc gccggtcccg
     1741 tctttgacca gaaaactggt ggcgctttcg ccggaatcga tggtcaccca ccgctcgttt
     1801 ttcccgctgc cttgtttttc ctgaacggaa tagcggaacc acacgcaggg ttgaagggac
     1861 aggggcgatt tcagggattt gcccgggagg ttttcggcca gcccgtcgat ttcgacgtac
     1921 ccctggggcg ccgaggcgat tttagcggtc ggcgtgtctt cgagcaagcg gtagcggcgg
     1981 aaactttcga gaaaccagag gaaggaaaac acgccgaggg cggccagaaa ggtcccccac
     2041 ccccaacacg cggccccgcc cgacagctcc gccttgagga gcaaataccc ccagatcgga
     2101 aataccgccg cggccgcggc ggcgagtttt ttcatgccga gaagaggttt ttgacgttga
     2161 cgtcctgggt ttcggcggcg tcgaatttca gaaactcaaa cgccttgaag gacccgaacc
     2221 gggccaccag ggaatccgga aattgctcga gccgggtgtt gttcaggttg acgctttcgt
     2281 tgtagagctc ccggcggtcg gaaatcgtgt tctccaggga ggacacccgg gtttggaggt
     2341 tgaggaaggt ctggttcgtt ttcaagtccg ggtagttttc ggcgacggcg aaaagaccgc
     2401 cgaggacgcc ccgcagctgc ccttcggcct gaccgagggc ggccacgttg ccggtttccc
     2461 gggcggaaaa gacggcggcc cgggccttca tgaccttttc cagcgtgtcc tgctcgaact
     2521 tcatgtattg cttgcaggtt tcgatgagtt tcggaagctc gtcgtgacgt tgcttcagga
     2581 cgatgtcgat gttggaccac gccttgccga cgttgttttt gaggcgcacc aacccgttgt
     2641 atacccccac gccgaacacc aacgccaaca ccaaccccgc cgacaccacg aacgccacga
     2701 tcataggtct tcctcctgag cgaacgaccc tctaagcgta atggaagttg tcccgtcgcg
     2761 caacgggaaa atcagtggat caaggaatga aaaaacgcgg ccgacccggt gaaaaagagc
     2821 gccgcggcga gccccaacgc gaacaccagc gttccgaccg ccaaagccgg gatcaacatc
     2881 aacagcaggg cgccgccccg ggaccatccg taaacgcgtt gaaggcccca ccaccaaagg
     2941 ccgtaggcca cgcaccggaa caagaggcgc ggcggcgacg ccaggagcgc gacgtccggc
     3001 gcggcggcgg ccaaaaggcg gaaaggcaat tccccccaga gcgccaaacc cgcccacccc
     3061 gcgacggaga ggaacgattc gaaccgccct tttttgcccg ccagccggga caacccgtgc
     3121 cccagagcgg ccaacagaaa ccacaggacc agacttcccg ccacggtggc cccgaaccgt
     3181 cgaacgagag cgacggcccc gtcgtcccag ggcaggggat cggacaggac ccggccggcc
     3241 acggccaagg tcatgacgag gagtcccgat ccgacccggg gatgggcggc cacgtcgtca
     3301 aaggcggccg ccggatcgac ggtcattcgt ccgatcaaat ccaaggcgtc cccggcggac
     3361 cgggcggggg tttcgttatt cccagacata gaggagggac ccgcgcacgc ccaacaaccg
     3421 ccgccattcg cccaaggggc cctcgaagag gccgccgaaa gcctccaacc accggtccca
     3481 agagcgaacg ggtccgatga tcttggggtg ggccgatttg atgcccgcca aagcggcggc
     3541 ttctttcaag gcgtcgtcgt aacttccgag ggcatcgacc aggccagcgg cccgggcctg
     3601 ctcgccggtg aacacccgtc cgtcggccag gggacgcagt tggtccaggg gcatcttccg
     3661 accccgggcc accgcctcca ggaattggcc gtaggcgctg gccaccagac cttcgaacac
     3721 ccggcgctcc tcggcgctca acggtcggta cggcgatccc atgtccttca tcgccccgga
     3781 tttgatgacc gtggcctgga cgccgatttt tttggtgagt tcctcgaaat tcatcaaatg
     3841 aaaaaccacg ccgatggacc cgaccaacga ccccgggttg gccacgatcc ggtcggccgc
     3901 cgcggcgacg taaaagccgc ccgacgccgc gatgtcgccc agggaggcca ccactttttt
     3961 tccgtccccc ttgagcgcca ggacggcccc gtggatttcc tgcacggagg c
//
LOCUS       DAOQLS010000112         3986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11321_length_3986_cov_4.193844, whole genome shotgun sequence.
ACCESSION   DAOQLS010000112 DAOQLS010000000
VERSION     DAOQLS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11321_length_3986_cov_4.193844"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1071)
                     /locus_tag="PK362_03065"
     CDS             complement(223..1071)
                     /locus_tag="PK362_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HNC73854.1"
                     /translation="MAPPDRTSFTFHGAVRSWNRNRRAVSIRGGVLDALRGTGYPSVV
                     HTFQGDDRTMCAQYIVDKSGKELARLFKALIRKVSEPRGRVVPHRPAPVVLREGDDRV
                     LTDMEFGLLPAWSQDRRVKFATHNARLFSEDSKTHRTVPIFQKPTWREPFARRHCLVP
                     MTQFIEPLYTGPLAGNMVGFLPRNGGVFAAAGLWDEWISKDSGEVVRSFTVLTDDPVP
                     HIAHMGHDRTPIFLGEGHFDRWLNASSHPPAGWIGFLRGNRESIDWIEAVDRPLAPGW
                     ERKASG"
     gene            1082..2020
                     /locus_tag="PK362_03070"
     CDS             1082..2020
                     /locus_tag="PK362_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC73855.1"
                     /translation="MLHRQLKRIEGISYFLLLIPVLTDLIERGRLPHEGRELVTEITL
                     GLIVLLFIKALQRARIKIERLETARQTLTELIVHDLKNPMTTVMASLSCLTDADYPEE
                     SRRKLLASALKSCNAQMELMDRLLEVERLESGLFPLRVQPIDLSNFLNGCLQEWTGPA
                     VLKEIELRTAPAYLAATVTADPELLRRVIGNLLQNALKYTPTGGRVELGATVEKDRVR
                     IRVADSGRGVGDEERKRLFDRYYRVEGRDQATHAGTGLGLYFCRLAVEAHGGTIAVAD
                     GAGMVVLIDLPLAPARTPPPVPSRPAIFGRTQPAAR"
     gene            2100..2672
                     /locus_tag="PK362_03075"
     CDS             2100..2672
                     /locus_tag="PK362_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73856.1"
                     /translation="MDTSLQLPQYLDLPNPVPGCNPGIHILRGPRFLPAWALRELCPH
                     LIRGARVAWIDAGNRFDAYGLAKAALALGAVPQRVLSRVQLARPFNAFQLVTMLAWKV
                     RALGLPVVLSDALGLFYDDELPLEEARRAFQSFLACLPETGGPLLCLLPEHRVSPDRN
                     FFTAEFLKRGKDVLTLEPAAEGFRIARGDR"
     gene            2672..2953
                     /locus_tag="PK362_03080"
     CDS             2672..2953
                     /locus_tag="PK362_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73857.1"
                     /translation="MGRTLPTQVQAQKDEREEWKLFRRALRKEDQEAFDALWRLARYH
                     AAPVAMGSQAVPLFGILMAMLVGLTRRVMDLEARLPGPSPHAPPERLDL"
     gene            2925..>3986
                     /locus_tag="PK362_03085"
     CDS             2925..>3986
                     /locus_tag="PK362_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73858.1"
                     /translation="MRRLNGWIFDAYPEGTGVRLWVLTPEGRHASFFDDWRPSFCVDG
                     DAGSVEAARRVLTKAGVPLETAWREKTDLYTGRSRRVLEARTPVGVHGRLVKTLRDLR
                     VPLFNADLPPIQHYHYERGHFPLARGEFEAEGDRLTAFHLRDDPWSVDYALPPLTTMH
                     LSLAGSEVAGALDPNHRALGSLRLLYEGNAYELEGSTEDQFESLERRLREWDPDVITS
                     DWGDSYLLPRLIEQSAGRGRPLPFSRDPRRTHAGRGSRSFMTYGRTVYQTGSVTLFGR
                     CHFDLKNSFYFKECGFDGLFEIARIAKIPLQRAARSTIGTSLSSMQLHRAHERNLLIP
                     IEKQQAEDFRPATELVVADK"
BASE COUNT          718 a         1295 c         1211 g          762 t
ORIGIN      
        1 gtgcggaaaa atttttcgat ggtcatcttc atgggaaact cctcttgggt ccttttaatt
       61 tccgtcgtcg ctcggtgacg aaaacaaatg ggggttcttc aacccacatg cttgcaacat
      121 tgtagccaaa atcgttttaa ttgcattgcg ggacatggcg aactaagctt agtccctcct
      181 ttgcatcatt tcaatagcta tatggattgc gcgaattgaa agttaacccg acgccttacg
      241 ttcccacccg ggcgccaggg gccggtccac ggcctcgatc caatcgatgg attcccgatt
      301 cccccgcagg aaaccaatcc aaccggccgg aggatgggag gaggcgttga gccatcggtc
      361 aaaatgcccc tcccccagga agatcggggt tcgatcgtgc cccatgtggg cgatgtgggg
      421 cacgggatcg tcggtgagaa cggtaaagga ccgcaccacc tcgccgctgt cctttgaaat
      481 ccactcgtcc cagaggccgg cggcggcgaa aacgcccccg ttccggggaa gaaagcccac
      541 catgttcccg gccaggggcc cggtgtagag cggttcgatg aattgcgtca tgggcaccag
      601 gcaatggcgc cgggcaaagg gctcccgcca ggtgggcttt tggaaaatcg gcacggtccg
      661 atgggttttg gaatcctccg agaaaagccg ggcgttgtgg gtggcgaatt tgacccgccg
      721 gtcctggctc caggccggaa ggaggccgaa ttccatgtcg gtcaaaacgc ggtcgtcccc
      781 ctcccgcaga acgaccggcg ccggccgatg gggcaccacc cgcccccggg gttccgagac
      841 cttccgaatc aaggctttga acagccgggc caactccttg ccggacttgt caacgatgta
      901 ttgggcgcac atggtgcgat cgtctccttg gaacgtgtgg acgacggaag gatagcccgt
      961 tccccgaagg gcgtcaagga cgccgccccg gatggacacc gcccggcggt ttcggttcca
     1021 cgaacgaacc gcgccgtgaa acgtaaatga agtgcgatcc ggaggcgcca tggaccaggg
     1081 aatgcttcat cgtcaactga aacgaatcga ggggatcagt tacttcctat tgctgatccc
     1141 cgtcctgacc gacctgattg agcgcgggcg ccttccccac gaggggcgcg agttggtcac
     1201 ggaaatcacc ttgggtctca tcgtgctcct ttttatcaaa gccctccaac gggcccggat
     1261 caagatcgag cggctggaga cggcgcgtca aaccctcacg gagctgatcg ttcacgatct
     1321 taaaaacccc atgaccacgg tcatggccag cctctcctgc ctcaccgacg ccgactatcc
     1381 cgaggagtcc cgcagaaagc tcttggcctc ggccctcaaa agctgcaacg cccaaatgga
     1441 acttatggat cggctgctgg aggtcgaacg gctcgaatcc ggactttttc ccctgcgggt
     1501 tcagcccatc gatttatcga attttctgaa cggctgtttg caggaatgga ccggcccggc
     1561 ggtcttgaag gaaatcgaat tgcgcacggc gccggcctat ctcgcggcga cggtgacggc
     1621 ggatcccgaa ctgcttcgac gcgtcatcgg caatttatta cagaacgcct tgaaatacac
     1681 ccccaccggc ggtcgggtgg agctgggcgc cacggtggaa aaagaccgcg ttcggatccg
     1741 ggtggcggac agcgggcggg gggtgggcga cgaagagcga aagcgcctct tcgaccgata
     1801 ttaccgcgtc gagggccgcg accaggcgac ccacgccgga acgggtctgg gactctattt
     1861 ttgccgtctg gccgtggaag cccacggcgg cacgattgcc gtggccgacg gcgccggcat
     1921 ggtcgttctg atcgacctgc ccctcgctcc cgctcggaca ccccctcccg tcccttcccg
     1981 tccggcgatt tttggcagaa cgcaacccgc cgcccgctga aacctggacc cgggatccct
     2041 acgaaagttc ttgcccttaa cagctataca tatgtatagt ctttcgaaac ggggggccca
     2101 tggacacgtc gcttcaactt ccgcaatatc tggatctgcc gaatcccgtc ccggggtgca
     2161 acccgggaat ccacattctt cgagggccgc ggtttctccc cgcctgggct ttgcgggagc
     2221 tctgccccca tttgattcgg ggggcccggg tggcctggat cgacgcgggg aaccgctttg
     2281 acgcctacgg attggcgaag gccgccctcg ccctgggggc ggttccgcaa cgggttctct
     2341 cgcgggtcca gttggcccgg ccctttaacg cctttcagtt ggtgaccatg ctggcctgga
     2401 aagtccgtgc cctgggactg ccggtggtgc tttcggacgc cctgggtctc ttttacgacg
     2461 acgaactgcc cctggaggaa gcccgccggg ctttccaatc ctttctggcg tgtctgcccg
     2521 aaacgggagg gccgctcctg tgccttttgc ccgaacaccg cgtttccccg gaccgcaatt
     2581 tttttaccgc ggaattttta aaacggggca aggacgtgct gaccctggaa cccgccgccg
     2641 aggggtttcg gatcgcgcgc ggggaccgct gatgggccga acgctcccca cccaggtcca
     2701 ggcccagaaa gacgagcggg aggagtggaa gctctttcgc cgggcccttc gaaaggaaga
     2761 ccaggaggcc tttgacgccc tgtggcgcct ggcccgttac cacgcggccc ccgtggccat
     2821 gggaagccag gcggtgcccc tcttcggcat tctgatggcc atgttggtgg gcttgacccg
     2881 gcgggtcatg gatctggaag cccgtctccc cggcccgtcc ccccatgcgc cgcctgaacg
     2941 gctggatctt tgacgcctat ccggaaggga ccggcgtccg tctctgggtt ctgacgcccg
     3001 agggtcggca cgcgtctttt ttcgacgact ggagaccctc cttctgcgtg gacggcgacg
     3061 ccgggagcgt cgaggccgcc cggcgggttc tgaccaaggc gggcgttccc ctcgagacgg
     3121 cctggcggga aaaaacggac ctctacaccg gacgatcccg ccgggtgctg gaggcccgga
     3181 cgccggtcgg cgttcacggc cgcctggtca aaaccctccg ggacctccgc gtgccgcttt
     3241 ttaacgccga cctccccccc attcagcact accactatga acgcggccat tttcccctgg
     3301 cccggggcga attcgaggcg gaaggcgacc gtctcacggc ttttcacctg cgggacgacc
     3361 cctggtccgt ggattacgct ctcccgcctt taaccacgat gcatttgagc ctcgcgggct
     3421 ccgaggtggc gggcgccctg gaccccaatc atcgcgccct ggggagcctc cggctccttt
     3481 acgagggcaa cgcctacgag ttggagggtt ccaccgagga tcaattcgaa tccctggaac
     3541 gccgcctgcg ggagtgggac cccgacgtca tcacctccga ctggggcgac agctatttgt
     3601 tgccccggtt gatcgaacag tcggccgggc gcggacgacc gcttcccttt tcccgggacc
     3661 cccggcggac ccacgccggg cgcggatccc gcagtttcat gacctacgga cgcaccgtct
     3721 atcaaacggg gtccgtgacg ctcttcggcc gctgccattt cgatttaaag aacagtttct
     3781 atttcaagga atgcggattt gacggcctct tcgaaatcgc gcgcatcgcc aaaatccccc
     3841 tccagcgggc ggcccgaagc accatcggca cctccctgtc ctccatgcag ttgcaccggg
     3901 cccacgagcg gaaccttctg atccccatcg agaagcagca ggccgaggat ttccggcccg
     3961 ccacggaact ggtggtggcc gacaag
//
LOCUS       DAOQLS010000113         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11400_length_3972_cov_4.758233, whole genome shotgun sequence.
ACCESSION   DAOQLS010000113 DAOQLS010000000
VERSION     DAOQLS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11400_length_3972_cov_4.758233"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK362_03090"
     CDS             <1..252
                     /locus_tag="PK362_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73859.1"
                     /translation="DVVAQVQPELEAERVRRRVENLFAKRSDLKNKPTVSLGVMAYAA
                     GVGGLAAYLEAADQALLQSKSLGKNRTILCEKPGTFRPV"
     gene            complement(254..691)
                     /locus_tag="PK362_03095"
     CDS             complement(254..691)
                     /locus_tag="PK362_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73860.1"
                     /translation="MRKAFWQILLAATLLGGAAGAEERLKASSLVKVFVGANGEKVTV
                     VFLEPIKRDRVLIKFEGVAGDWEGKVILHKRVQDSWSDDYETVVKGERFVSLVGRSRQ
                     GVMSYQLYPLGLRTQGIAVTYSAEESKRALGAKIVYEFTSPAP"
     gene            complement(684..2147)
                     /locus_tag="PK362_03100"
     CDS             complement(684..2147)
                     /locus_tag="PK362_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNC73861.1"
                     /translation="MRTPAGLKKKILALDKASGGGLWLVGGFVRDALLRRPTADVDVA
                     VAGDARALAEKFARAEKRRAFALDEERGTYRVVVEDDGQKMDFDFSRLQGKTIEEDLG
                     RRDFTANAMALTVADWAAGRWARTIDPFDGRRALKKKEIALVSPRALDEDPLRLLRAF
                     RFSAELGWAVAPGAFKEIKKRAKKILRSSPERIREELMKTLAAPVGSRAFFDLDKAGL
                     LTVLFPEGEPMRRTGKTYYGAGGVLRHSLESMASMEEVLGRLPTYFPKFHRPLAAYLN
                     ERLGGHPRYAHLKLVELFHDVGKPATAKVRDGKLHFYGHDAVGAKMVKRIAERLRFST
                     NEGRSLTRLVGAHMRPGNLGHAPQLTERAIFRFYRDLQEDAVAMLIVALGDHFTYLSE
                     RVKRGGKDPVFLTIRKMLEAFFLKRATVEPPRVLDGNVLMKTLGIKPGPEVGRLLDAV
                     REAQAAGQVTGRDEALALARRLHAAAPKVKKIGGSHA"
     gene            complement(2144..3703)
                     /locus_tag="PK362_03105"
     CDS             complement(2144..3703)
                     /locus_tag="PK362_03105"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNC73862.1"
                     /translation="MTAKPHTLTAALRSTVRAHPERIAFYFRDRTWTYGELEDRVARC
                     ARGLHRLGLRPGDACGLVLRNCPEFVVLFFALCRLGAVAVPVNFLEKGEKLGYIFADA
                     GVKACLTSKEFLKQVHEAQKKAPHLRHVFVREPAGHHPTFESLLAGEGGNFDEPALGG
                     DALAMLIYTAGTTGNPKGVMLTHGNFLANVESCHRAIEVGPEDRFLCLLPMFHSFSWT
                     VNVLLPIRLGATIVILETLLPFEPVLKTIWERKITVFCGVPPLFAALTQKIQGWKALL
                     VRFLNPVRVAISGASPLPAAVHREFEAKFRRPLIEGYGLTEASPVVAVNPLHKERKPG
                     TVGLPLPGVHVKIIDDYDRPCPTGEVGEVCVKGANVMAGYFNHPEETRAAVTEDGWLK
                     TGDLGCLDAQGYLTIVDRKKDLIIVKGLNVYPQEVEAVLTSHPDVAEAAVVGVPDETG
                     DETVLAFAAAHKGKTLDTAALLSLCREKLASYKAPKELFVKTELPKNAMGKVLKKELR
                     QEAARLRRRRS"
     gene            complement(3700..>3972)
                     /locus_tag="PK362_03110"
     CDS             complement(3700..>3972)
                     /locus_tag="PK362_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC73863.1"
                     /translation="RLLLADEPVSSLDLSVQAQILNLLADLREKFNLAVILISHDLTV
                     VRQLTDRVLVLAGGRLAEEGETARVLAAPQSPVTRNLLAAVPALLR"
BASE COUNT          720 a         1345 c         1196 g          711 t
ORIGIN      
        1 gacgtggttg cgcaggtcca gcctgagttg gaggcggagc gggtccgccg gcgggtggaa
       61 aatctatttg cgaagcgaag cgatctcaag aacaagccga ccgtgagcct gggcgtcatg
      121 gcctacgcgg ccggggtcgg cggcttggcc gcctatctgg aagcggcgga ccaagccctc
      181 ttacagtcca aatccctcgg caaaaaccgc accatcctct gcgaaaaacc cggaaccttc
      241 cgccccgtct aaattagggc gcgggcgagg tgaattcgta tacgatcttg gcccccaggg
      301 ccctcttgga ctcttcggcc gaatacgtca ccgcgatgcc ctgggtgcga aggcccaggg
      361 gatagagttg ataagacatg accccttgcc gggaccggcc caccagcgac acgaatcgtt
      421 cgcctttgac caccgtttcg taatcgtcgg accaggagtc ctgcacgcgt ttgtggagga
      481 tcaccttccc ttcccaatcc cccgccaccc cttcaaactt aatcagcacc cggtcgcgtt
      541 tgatgggctc caaaaaaacc acggtcactt tttccccgtt ggcccccacg aacaccttca
      601 ccagggacga ggccttgagc cgctcctccg cccccgccgc gccgcccagg agcgtcgccg
      661 ccaacaggat ttgccaaaac gccttacgca tgggaacctc cgattttttt taccttgggt
      721 gcggccgcgt gcaaccgacg ggccagggcc agggcttcgt cgcggccggt cacctgcccc
      781 gcggcctggg cttcgcggac ggcgtccaat aggcgcccca cctcgggccc cggcttgatc
      841 cccagggttt tcatcagaac gtttccgtcc aggaccctgg gcggctccac cgtcgcgcgc
      901 ttcaaaaaga acgcctccag cattttgcgg atggttagaa acacggggtc tttccccccg
      961 cgcttgaccc gctccgacag ataggtgaaa tggtcgccca gggccacgat aagcatggcc
     1021 accgcgtcct cctgaaggtc ccggtaaaaa cggaaaatcg cccgctccgt cagctggggc
     1081 gcgtggccca ggttgccggg gcgcatgtgg gcccccacca gtcgggtcag ggaccggcct
     1141 tcgttcgtgg agaaccgcag gcgctcggcg atgcgcttga ccatcttggc ccccacggcg
     1201 tcgtggccgt agaagtgcag tttcccgtcc cgcaccttgg ccgtggcggg tttccccacg
     1261 tcgtgaaaca gctcgaccaa cttgagatgg gcgtaacggg gatgcccccc caggcgttcg
     1321 tttaaatagg cggccagggg ccggtggaat ttggggaaat aggtcggcaa ccgccccaac
     1381 acttcctcca tggacgccat ggattccagg gaatggcgca acacgccccc cgccccgtaa
     1441 taggttttcc ccgtgcgccg catgggttcg ccttcgggaa aaagaaccgt caggaggccc
     1501 gctttgtcca ggtcgaaaaa ggcccggctc cccacggggg cggccagggt tttcatcaat
     1561 tcctcccgaa tccgctccgg ggaggaacga agaattttct tggcgcgctt tttgatttcc
     1621 ttgaaggccc cgggcgccac cgcccagccc agctccgccg aaaaccggaa ggcgcgaaga
     1681 agccgcaggg ggtcctcgtc cagggcccgg ggcgacacca gggcgatttc ctttttctta
     1741 agggcccggc ggccgtcgaa agggtcgatg gttcgggccc aacgcccggc ggcccaatcc
     1801 gccacggtca gggccatggc gttggcggtg aaatcccgtc ggcccaaatc ctcctcgatg
     1861 gttttcccct ggaggcggga aaaatcgaaa tccatttttt gaccgtcgtc ttccaccacc
     1921 acccgatagg tgccgcgctc ctcgtccagg gcgaaggccc ggcgtttttc ggcccgggcg
     1981 aatttttccg ccagggcccg ggcgtccccg gccaccgcca cgtccacgtc cgccgtgggc
     2041 cggcgaagaa gcgcgtcccg cacaaacccg cccaccagcc acagcccccc gccggaggcc
     2101 ttgtccaggg cgaggatttt tttcttgagt cccgccgggg tcctcacgat cgtctccgcc
     2161 gaaggcgcgc ggcttcctgc cgcagttcct ttttcaacac ttttcccatg gcgtttttcg
     2221 gcaattccgt tttcacaaaa agctccttgg gcgccttgta ggacgccaac ttttcccggc
     2281 agagggacag caacgccgcc gtgtccaggg ttttcccctt gtgcgccgcg gcgaaggcca
     2341 gcacggtctc gtcgccggtt tcgtcgggca cgcccaccac cgcggcctcg gccacgtccg
     2401 ggtgcgacgt taaaaccgct tccacttcct gggggtagac gtttaggcct ttcacgatga
     2461 ttaaatcttt tttccggtcc acgatggtca aatacccctg ggcgtccagg caccccaggt
     2521 ccccggtctt cagccacccg tcctcggtga cggcggcccg ggtctcttcc ggatgattga
     2581 aatacccggc catgacgttg gcgcctttca cgcagacctc gccgacctcc cccgtgggac
     2641 agggccggtc gtagtcgtcg atgattttga cgtggacgcc cggaaggggc aggcccacgg
     2701 tgccgggctt ccgttctttg tgcagcggat tgaccgccac caccggcgac gcctcggtca
     2761 gcccgtagcc ttcgattaaa ggccgacgaa atttggcttc gaattcccgg tgcaccgccg
     2821 ccggcaaggg cgacgcgccg gagatcgcca cccgcaccgg gttgagaaac cgcacgagca
     2881 acgctttcca accctggatt ttctgcgtca gcgccgcgaa gaggggcggc accccgcaga
     2941 agaccgtgat tttccgctcc cagatggttt tcaataccgg ctcgaagggc aacagcgttt
     3001 ccaggatcac aatggtggcg cccaggcgga tgggaagaag gacgttcacc gtccaggaaa
     3061 acgaatggaa catgggcaag aggcacagaa accggtcctc gggtcccacc tcgatggccc
     3121 ggtggcagga ctccacgttg gccagaaaat taccgtgggt cagcatgacc cccttggggt
     3181 ttcccgtggt gccggccgtg tagatcagca tggccaaggc gtcgccgccc agggccggct
     3241 cgtcgaagtt gccgccctcc cccgccagca gggattcgaa ggtcgggtga tggcccgccg
     3301 gttcccggac gaacacgtgg cgcaggtgcg gagccttctt ttgcgcctcg tggacttgct
     3361 ttaaaaattc cttggaggtg aggcaggcct tgacccccgc gtcggcgaaa atgtaaccga
     3421 gcttctcccc cttctccaaa aagttcaccg gcaccgccac cgcgcccaac cggcacaggg
     3481 cgaagaagag cacgacaaac tccgggcagt tgcgcaacac caacccgcag gcgtcgcccg
     3541 gccgcaggcc cagtcggtga aggccccggg cgcaacgggc cacccgatcc tccaattccc
     3601 cgtaggtcca ggtccggtcg cgaaaataaa aagcgatgcg ttccgggtgg gcccggaccg
     3661 tggatcgaag ggcggcggtc aaggtgtggg gtttggcggt catcggagaa gcgccggcac
     3721 cgcggccaaa agattccgtg tgacggggct ttggggcgcc gccaacaccc gggcggtttc
     3781 gccttcctcg gccaaacgcc cgcccgccaa caccaggacc cggtccgtca attggcggac
     3841 caccgtcagg tcgtggctga tcaggatcac cgccagattg aatttttccc gcaggtccgc
     3901 cagcaaattc aagatttggg cctgaaccga caggtccaag gacgacaccg gttcgtccgc
     3961 caacagcagc cg
//
LOCUS       DAOQLS010000114         3971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11414_length_3971_cov_3.342952, whole genome shotgun sequence.
ACCESSION   DAOQLS010000114 DAOQLS010000000
VERSION     DAOQLS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11414_length_3971_cov_3.342952"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3349)
                     /locus_tag="PK362_03115"
     CDS             complement(<1..3349)
                     /locus_tag="PK362_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73864.1"
                     /translation="MAVHVQVHWITYDAKGREHQKNQSVQWTNSTYGMVPGLSDGRVN
                     GWVNGNATVTITNAYDNADRLVDQQASGTVHSAYNANLAQHEGYFSAFGFSSHQYNMK
                     YDVYGHLVYNDYSNYMPGTTVVGCGKHCSKTVDTMLWTSGGTSSSDFDEFGRAHRVYN
                     SYSSSDGSNGWQNTYDIVYDNATGNVLTQSEQYHSYSPPVGKGGATVTDGLKIVTNHY
                     DDRNELTTRDENKVWEHTETQGGGGCMDGIMGKLLMAVIMIAAIYIGVGFAINFQWAA
                     LGENLASMSLLDAAAAVEVGGVAVGAATVLTCVAVSTFVVSFAMTYMMTGNLEMSLIS
                     GLMAAASSVATLYMGSASFMNGGETSTAATTTSQSALGSSEGFLNEMASSISKNIASI
                     FDVTGKAVNVITVAVKDFIRQAITIVVSDTFQQYAPDLGYLTGTITQGLLAFGGMGDL
                     SKMLDTKILLQKAAVIAAGQLIDKALGPKNGWMSSLLMVAVGFKQGGTSAEGFSLLDP
                     SKRDLWRGLMNSLATKANQLLQKRYAGEMSTFTKIVDGKVVGSKTTAAGFLLTDSVRN
                     IGTFLGEQAARSFAPLESAPSAKAQTTMVDLAQANLPSSQAGLATMAFADLALTAANG
                     DRAAGAAFRSSDDGQSANQQRTATLSDIESGKFNKELAGNADFVAAGKALGISDFESK
                     LLKDSFNPIQLMMDSWSNPDRIQAQATEPQNDGERRIAVLDLKNLDAQSKANLLSLTK
                     DELKSVFSSYDLTQVIGPVKLGLLGNDKVLIFEIKGSGIKEKETSKFIDRAGLKDAVV
                     RVVHDLDSGKIFLDLTKQFKDGVIKGDEAVAKFMGFLKAAYGDDKSGIFKALSENLKA
                     LGGASFLEGATVKKFLVSVNKGVDGTVEIGKSFDSLAGSPLQGLAAGLGYGKGQEVSF
                     QIDLKSGGIYMVSAAPMQIAGVTKDLTSKLTSAGVSSDDANKIKAALNAEAQGAFKLG
                     VDLKLTARTMENGTIEGARVRFEGDVNLVNDATLNLGSLRGAGGEVSFTLTVDQGKAS
                     VGDQIDLKLNGQQASTLVQKVQNNEIKLDGRPLEAILKELQNLEASGFKEMTVHITKT
                     GEGDKVQTRETVSVKVDESKVSAEIKAQ"
BASE COUNT          798 a         1106 c         1078 g          989 t
ORIGIN      
        1 actgggcctt aatctcagcg ctcaccttgg attcatcgac tttaacggaa accgtttcgc
       61 gggtttggac cttatccccc tcccccgttt tggtgatatg aactgtcatt tctttgaaac
      121 cgctggcctc cagattctga agttctttga gaatggcttc cagcgggcgg ccgtcaagct
      181 tgatttcatt gttttgaact ttttggacca aggtgctggc ctgctgcccg ttcaatttca
      241 aatcaatttg atccccaacg gaggctttgc cttggtcaac ggtgagggtg aagctcactt
      301 cgccgcccgc gccacgcagt gaaccgagat tgagggtcgc gtcgttgacc aggttgacat
      361 ccccttcgaa gcgaacccga gcgccttcga tcgtcccatt ttccatggtt cgggccgtca
      421 gtttgaggtc gacacccaat ttgaaggcgc cttgggcttc ggcattgagc gccgctttga
      481 ttttgttggc gtcatccgaa gaaactccgg ccgaagtcaa tttgctcgtc aagtctttgg
      541 tcacgccggc gatttgcatc ggcgcggcgc tgaccatgta aattcccccg gattttaggt
      601 caatctggaa agaaacttcc tgcccctttc cataacccaa accagcggca agtccttgca
      661 gagggcttcc ggccaaacta tcgaaagatt tcccaatttc gacggttcca tccactcctt
      721 tgttaaccga gaccaagaac ttcttaaccg tggcgccttc caaaaagctg gcaccaccaa
      781 gagctttcaa gttttccgac aacgctttaa aaatgcccga cttgtcgtcc ccgtaggccg
      841 ctttgaggaa gcccatgaat tttgccacgg cttcgtcgcc tttgatgacg ccgtctttga
      901 attgttttgt caagtccagg aaaattttcc cggaatccaa gtcgtgaacg acccgaacca
      961 ccgcgtcctt aagtccggcg cgatcgatga acttggaggt ttccttttct ttgatgcccg
     1021 atcccttaat ctcgaaaata agcactttgt cgttccccaa aaggcccaat ttcacggggc
     1081 cgatcacctg ggttaagtcg taactggaga ataccgattt caactcgtcc ttggtgaggg
     1141 acagcaagtt ggctttgctt tgtgcgtcga gatttttcag gtccaaaacc gcaatccgcc
     1201 gctcgccgtc gttttgaggt tcggtggcct gggcctgaat gcgatcgggg ttgctccagg
     1261 aatccatcat aagttggatg gggttgaacg agtcctttag caacttggat tcaaaatcac
     1321 taatcccaag ggctttgccc gcggcaacaa aatccgcgtt tcctgccaat tccttgttga
     1381 atttgcccga ttcgatgtcg ctcaaggtgg ccgttcgctg ttgattcgcc gattgaccat
     1441 cgtccgagga acggaacgct gccccggcgg cacgatctcc attggccgcg gttaaagcca
     1501 ggtcggcaaa agccatcgtc gccaaccccg cctggctgga cggtaaattg gcttgcgcca
     1561 agtccaccat cgtggtctgt gccttggcgc tgggagcgct ttccaaagga gcaaaactac
     1621 gcgccgcctg ttctccaagg aaggtcccaa tgtttcgaac gctgtcggtt aaaaggaagc
     1681 cggccgccgt cgtttttgag cccacgacct tcccgtcgac tattttggtg aaggtgctca
     1741 tctcgcccgc gtagcgtttt tgaagcaact gattggcttt ggtggccagg gaattcataa
     1801 ggccgcgcca aagatcgcgt ttggagggat caaggagcga gaacccttcg gccgaagttc
     1861 cgccctgctt aaatccaacg gcgaccatca acagcgaact catccaaccg ttcttgggcc
     1921 caagcgcttt gtcgattaac tggccggccg caatgaccgc ggccttttgc aaaagaattt
     1981 tcgtgtccag catttttgat aaatcgccca ttcctccgaa agccaaaagc ccctgggtga
     2041 ttgttccggt taaataaccc agatccggag cgtattgctg gaaggtgtcc gataccacga
     2101 tggtaatggc ttggcggata aaatccttaa cggcaacggt gatcacattg accgctttac
     2161 cggtcacatc aaaaatactc gcaatattct tcgaaatgct actggccatc tcgttaagaa
     2221 acccttcgct cgaacccaga gcgctttggc tggtcgttgt tgcggccgtg cttgtttccc
     2281 cgccattcat gaaggaggcg cttcccatgt aaagggtggc aacggaggat gccgcggcca
     2341 ttaagccgga gatcaaagac atttcgagat tgccggtcat catgtaagtc atcgcaaagc
     2401 tgaccacgaa ggtcgacacc gccacgcagg taagaacggt cgcggctcca acagcaacgc
     2461 cgccgacttc caccgcggcc gccgcgtcaa gcagggacat ggaagccaag ttttcaccca
     2521 aagccgccca ttggaaatta atagcgaacc cgacgccaat gtaaatggcg gcgatcataa
     2581 tgacggccat gagcagtttg cccataatac cgtccatgca accgcctccg ccttgggttt
     2641 cggtgtgttc ccagactttg ttttcgtccc gggtggtcag ctcgttgcgg tcgtcgtaat
     2701 ggttggtgac gatcttcagc ccgtccgtta ccgtggcgcc gcctttgccc acgggaggcg
     2761 agtagctgtg gtactgttcg ctttgggtca gaacgttacc ggtggcgttg tcgtaaacga
     2821 tgtcgtaggt gttttgccag ccgttgctgc cgtcagagct ggagtaggag ttgtagaccc
     2881 ggtgagcgcg cccaaactcg tcgaagtccg aggagctcgt ccccccgctg gtccagagca
     2941 tcgtgtcgac ggtcttgctg cagtgtttgc cgcagccgac caccgtcgtc ccgggcatgt
     3001 aattggaata atcgttgtac accaaatgcc cgtagacgtc gtatttcatg ttgtattggt
     3061 gagaggagaa tccaaaagcc gagaaatacc cttcgtgttg ggcaaggttg gcgttgtagg
     3121 cactgtgaac cgtcccgctg gcttgttgat cgaccaaacg atcggcgttg tcgtaggcgt
     3181 ttgtgattgt gaccgtcgcg ttgccgttga cccagccgtt cactcgtcca tcgctgagtc
     3241 cggggaccat tccgtaggtg gagttggtcc attggacgct ttggttcttc tggtgttccc
     3301 gccctttggc gtcgtaggta atccagtgca cctgcacatg gacggccacg ccgccgtttt
     3361 tcgaataggt ttcgttgtag gagtatgttt gcccgagggc gttgtaggtg atgttggtcc
     3421 gaaccgtatc ggacgtcccc ttgccctggt cccaccggtg ttccgtgtag gccgagacgc
     3481 ggccgttgct gtcgtaggaa ttgatcgttc ggtcggaagt gaatcggccc atcccgtccc
     3541 gataaccctc ggtgtggatg gttccgccgg cgatgcgccg cccgttggag tcgtagcgat
     3601 aggtttcggt cgtgtcgctc caggagctga cggtcacatt tcgaaccgtc gtcgagtggc
     3661 ggatcgaaat ggttccgtcg ccgttgtagt aactgccgcc ttcgccgtca tacgacatgg
     3721 tccccatgtc gacggccaac gaactggagg attgggcttc gctgtagatc gtgctcgtct
     3781 caatgtgcga acgagattcg atgattttcg ccgaaccggc gaaaatgccc attttttggt
     3841 gaatcgtccc gctgcccgaa acatacccgc cgacaatgga ccagcccgat gtcgaactcg
     3901 acccttcggg caaggatccg tgctgctggt agtattcgag gcaggattcg tgggagaccg
     3961 tttccgatgt t
//
LOCUS       DAOQLS010000115         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11437_length_3966_cov_5.017387, whole genome shotgun sequence.
ACCESSION   DAOQLS010000115 DAOQLS010000000
VERSION     DAOQLS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11437_length_3966_cov_5.017387"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PK362_03120"
     CDS             <1..520
                     /locus_tag="PK362_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC73865.1"
                     /translation="MGLSEDFCESLFLASPMHDIGKLGVPDAILRKPGRLDEKEWLEM
                     RKHPVYGADILSHSENELLQMSERVARSHHEKWDGTGYPDGLKGEAIPLEARIVALAD
                     VFDALTSKRCYKPAFTLSDSLKIIEDGAGQHFDPRVVAAFKKVVPEATRVMEEFSDKP
                     EDPPPFFDGRRP"
     gene            complement(517..2556)
                     /gene="uvrB"
                     /locus_tag="PK362_03125"
     CDS             complement(517..2556)
                     /gene="uvrB"
                     /locus_tag="PK362_03125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNC73866.1"
                     /translation="MKAFEVVSRFSPSGDQPAAIEQLVRGLRAGEKHQVLLGVTGSGK
                     TFTMANVIAALDCPTLVLSPNKILAAQLYAELKAFFPKNAVEYFISYYDYYQPEAYVP
                     QSDTYIEKDASINDHIDRLRLKATSSLLERRDVIVVASVSAIYGLGSPREYKDLCVGL
                     EVGAPRERDDVTRGLVDIQYERNEIDFSRGKFRVKGDVVDIFPAYLETALRVEFEGGI
                     LKRLREINPLTGETLQEKERTFVYPAKHFVTTGPTIDRALEEIRRELSGRLAVLRGEG
                     KLLEAQRLEQRTRFDMEMMKELGFCHGIENYSRPLSGRKPGERPACLFDYFPSDYLVV
                     IDESHVTVPQIGGMYEGDRSRKQTLVDYGFRLPSALDNRPLKFPEFEALVPRVVYVSA
                     TPGPYELKKTGGAVVEQVIRPTGLVDPEVLLRPIQGQVEDLVGRLQERVKRRERVLVT
                     TLTKKTAEDLSDYLEGRGLRVRYLHSDIESLRRIEILKDLRRGTFDVLVGINLLREGL
                     DLPEVSLVAVLDADKEGFLRSETTLIQVCGRAARNVNGQVVLYADKITGSMERAMGEM
                     DRRRKKQLAHNAAEGITPKTIVSAVHDLEEFQIKAKESQLPGLFREAGAAYLTRKELP
                     GLIKQLETQMREAAEALDYELAAVIRDKMMEIKDMMATGRGVRSGPAAKGRGRKS"
     gene            complement(2690..3187)
                     /locus_tag="PK362_03130"
     CDS             complement(2690..3187)
                     /locus_tag="PK362_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC73867.1"
                     /translation="MVRKRKTAFTLVEVLVASAIMGVIAFLVPQALSKIYQLFTVSQA
                     RIECQRDLRDCVFQIEKDLNQARSSTVRVSRDINQPVFSKIVFQTVAGSTTTYRQDGR
                     RLIVNRGGGDLALTRNLMSVAFLLNEMGQTRSVHVALVVGKDGDRLHKKTFSTLLRNI
                     VFKNS"
     gene            complement(3181..>3966)
                     /locus_tag="PK362_03135"
     CDS             complement(3181..>3966)
                     /locus_tag="PK362_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73868.1"
                     /translation="VVDYAYYKEKATLTRLPASTAGGSIQIFPTRSDNVIFVPNGAAP
                     VSNPVGSGYFPGSDNVLNQGVIGNDQLILGFMNNGGGGYVFQNSTSVVFVAGREINAE
                     AYVFLDTGTFLDVEALVVTGMNYSAFVYAAGAGGGVVVASVPAGADLEYASAAGLAVW
                     NAGGALSMATAFAQPNHCCYTINNVNVRGLVITEDLLPNNHAGVFVGGVVSDGAGSHC
                     GDVFGVSGRTYRDAIYYQPAFMDNLKTLNTPIRKTWKEVTASW"
BASE COUNT          760 a         1251 c         1178 g          777 t
ORIGIN      
        1 catggggttg tcggaggact tttgcgaaag ccttttcctg gccagtccca tgcacgacat
       61 cggaaaactg ggcgtgcccg acgccatttt gcgcaaaccc ggacggttgg acgagaagga
      121 gtggctggaa atgcggaaac accccgttta cggagcggac atcctgagcc actcggagaa
      181 cgaattgctc caaatgtcgg aacgcgtggc ccgctcccac cacgaaaaat gggacgggac
      241 cggctacccg gacgggctca agggcgaggc gattcccctg gaagcgcgca tcgtggccct
      301 ggccgacgtc tttgacgcgc tgacctccaa gcgatgttac aaacccgcct tcacgctgag
      361 cgacagcctg aaaatcatcg aagacggagc gggccagcat ttcgaccccc gggtggtcgc
      421 ggcgtttaaa aaggtcgtgc ccgaagccac ccgcgtcatg gaagaatttt cggacaagcc
      481 cgaggacccg ccgcccttct tcgacggtcg ccgcccctaa cttttccttc cccgcccctt
      541 cgccgccgga cccgaccgga cgccccgccc ggtggccatc atgtccttga tttccatcat
      601 cttgtcccgg atcacggcgg ccaattcgta gtcgagcgcc tccgccgcct cccgcatttg
      661 ggtttccaat tgtttgatca atcccggcaa ttccttccgg gttaagtagg cggccccggc
      721 ctcccggaaa agtcccggca gctgggattc cttggccttg atctggaact cctccaagtc
      781 gtgaacggcc gagacgatgg ttttgggcgt aatgccctcg gccgcgttgt gggccagctg
      841 ttttttccgg cggcggtcca tttcccccat ggcccgctcc atggacccgg tgattttatc
      901 ggcgtaaagg accacctggc cgttgacgtt ccgggcggcc cggccgcaca cctggatgag
      961 cgtcgtttcc gaacggagaa atccctcttt gtcggcgtcc agcacggcga ccagcgaaac
     1021 ctccggcagg tcgagcccct cccgaaggag gttgatgccc accaacacgt cgaaggtccc
     1081 ccggcgcagg tccttcagga tctcaattcg tcggagggat tcgatgtcgg agtgcaggta
     1141 gcgaacccga aggccccggc cctccagata atccgataaa tcctccgccg tctttttcgt
     1201 caaggtggtg accaacaccc gctcccgccg cttcacccgc tcttggaggc gcccgacgag
     1261 gtcttcgacc tgcccttgaa tggggcgcag aaggacttcc gggtccacca gtcccgttgg
     1321 ccgaatcacc tgctccacca cggccccgcc ggtttttttc aattcgtagg ggcccggcgt
     1381 ggcgctcacg tagacgaccc ggggcaccag ggcctcgaac tccggaaatt tcaacgggcg
     1441 gttgtcgagc gccgagggca gccggaaccc gtagtccacg agggtctgct tccgggaccg
     1501 gtcgccctcg tacatcccgc cgatttgggg caccgtcacg tgggattcgt cgatgacgac
     1561 caggtaatcc gacgggaaat aatcgaaaag gcaggcgggg cgttccccgg gcttccgccc
     1621 cgagaggggc cgggaatagt tttcgatgcc gtggcaaaac cccagttcct tcatcatctc
     1681 catatcgaaa cgggttcgtt gctcgagccg ctgggcctcc agcagtttgc cttcgccccg
     1741 caggacggcc agacgaccgg acaattcccg ccgaatctct tccagggcgc ggtcgatggt
     1801 cgggcccgtc gtcacgaagt gtttggcggg gtacacaaag gtgcgctctt tttcctgaag
     1861 ggtttcgccc gtcaagggat tgatctcccg aagacgcttg agaatcccgc cctcgaattc
     1921 cacccgcagg gcggtttcca aataagccgg gaaaatatcg accacgtccc ctttgacgcg
     1981 gaacttcccc cgggaaaaat cgatttcgtt ccgttcgtat tgaatgtcca ccagcccccg
     2041 ggtgacgtcg tcccgttccc ggggggcgcc gacttccagg cccacgcaga ggtctttgta
     2101 ctcccgggga ctgcccaaac cgtaaatggc cgaaaccgag gccacgacga tcacgtcccg
     2161 gcgctccagc agcgacgaag tcgcctttaa ccgcagtcga tcgatgtggt cgttgatcga
     2221 tgcgtctttc tcgatgtagg tgtcgctctg gggaacgtag gcttcgggct gatagtagtc
     2281 gtagtaggaa atgaagtatt cgacggcgtt cttcgggaaa aaagccttga gttcggcgta
     2341 gagttgggcg gcgaggattt tattgggcga gaggaccagg gtggggcagt ccagggcggc
     2401 gatgacgttg gccatggtga acgttttgcc gctgccggtg acccccagaa ggacctggtg
     2461 cttctccccc gcccgcaacc cccggaccaa ttgttcgatg gccgccggtt ggtcaccgga
     2521 gggtgaaaat cgggaaacga cttcgaacgc cttcatggat cggggcccct ttccaccgcc
     2581 acggccgcga cccacgtccg cccgccggag ggaagtgtcg cccattgtaa ccttctcatc
     2641 ggccggtgac cagaccgacc gtcggccacg ggcggattcg aggcggggcc taactgtttt
     2701 taaagacgat gttacgaagt agcgtggaga aggttttttt gtgcagacgg tcgccgtcct
     2761 tccccaccac gagggcgacg tgcacggaac gggtttgccc catttcgttc aagagaaacg
     2821 ccaccgacat cagattgcgg gtcagcgcca aatccccccc gccccggttg acgatcaacc
     2881 ggcgtccgtc ttgccggtac gtcgtcgtcg agccggcaac ggtttggaac acaatctttg
     2941 aaaacacggg ttgattgatg tcccgggaca cccggaccgt gctggaacgg gcttgattaa
     3001 ggtctttttc aatttgaaag acgcaatccc gaagatcccg ttggcattcg atgcgggcct
     3061 ggctcacggt gaacaattgg tagatcttgc tcagggcttg gggcaccaag aacgcaatca
     3121 cccccatgat cgccgacgcc accaggactt caaccaacgt aaacgccgtt tttcgttttc
     3181 ttaccacgac gcggtcactt ccttccaggt ttttcgaatc ggcgtgttca acgttttgag
     3241 attgtccatg aaggccggct ggtaataaat ggcgtcgcgg taggtgcgcc ccgacacgcc
     3301 aaacacgtcg ccgcagtgcg atccggcccc gtccgaaacc accccgccca caaacacgcc
     3361 ggcgtggttg ttcggcagga ggtcttcggt gatgaccaac cctcgcacgt tgacgttgtt
     3421 gatcgtgtaa caacaatggt tcggttgggc gaaggcggtg gccatgctca aagccccgcc
     3481 ggcgttccag accgcgaggc cggccgccga agcgtattcc aaatccgcgc cggccgggac
     3541 ggaggccacc accacgccgc ccccggcgcc cgcggcgtaa acaaacgccg aatagttcat
     3601 ccccgtcacc acgagggcct cgacgtccag gaacgtcccg gtgtcgagga agacgtacgc
     3661 ctcggcgttg atttcgcgcc cggcgacaaa caccaccgag gtggagtttt gaaacacgta
     3721 gccccccccg ccgttgttca tgaacccgag aatcagctga tcgttgccga tgacgccttg
     3781 attcaagacg ttgtccgacc cggggaaata gccgcttccg acgggattgc tgacgggggc
     3841 ggccccgttg ggaacgaaaa tgacgttgtc cgatcgggtc ggaaagattt gaatggaacc
     3901 accggcggtg ctggcgggca aacgggtgag cgtggccttt tccttgtagt acgcgtaatc
     3961 gactac
//
LOCUS       DAOQLS010000116         3961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11463_length_3961_cov_4.997184, whole genome shotgun sequence.
ACCESSION   DAOQLS010000116 DAOQLS010000000
VERSION     DAOQLS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11463_length_3961_cov_4.997184"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PK362_03140"
     CDS             <1..762
                     /locus_tag="PK362_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73869.1"
                     /translation="VIGSARRFASHTYNRRLEDVDPFPIFVFDKGGVTDAAEFVATHE
                     KALTGIVSGEPNYDALSDFVLAQEKIRNFGYYENELILIKRFGAVVSSEESGTIVELI
                     RLAYSLYWSLRAYNFFLEKEIDQAQELLADLPPYYKFWAMPSRYQTFSREAMDFVRDK
                     LAIVDSLHNVQANIPRIDSDWHLRTIYKNVEKEFDIDELSKAVDTKLERIEQSYNSAR
                     DFISTNFFIAVEIVLILSLAWMMLDTGLLFIIAHK"
     gene            884..1405
                     /locus_tag="PK362_03145"
     CDS             884..1405
                     /locus_tag="PK362_03145"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNC73870.1"
                     /translation="MSDASVEYTLVVVKPDGLKKSLTGNILTKLAEARFMIIGAKVLR
                     VSRELAEQHYQHLKGKPFFNDLIDYIQGKPYGPDFERVMALVYKGPNAIKKIREIAGA
                     TNPEEADSVSIRGAFGRITTKGVFENVIHASSDAKDSEREIKLWFRPEEIVGEIYPTK
                     KVQSQAHEVVAWA"
     gene            1609..1681
                     /locus_tag="PK362_03150"
     tRNA            1609..1681
                     /locus_tag="PK362_03150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Ala,seq:ggc)
     gene            1828..2406
                     /gene="rsmD"
                     /locus_tag="PK362_03155"
     CDS             1828..2406
                     /gene="rsmD"
                     /locus_tag="PK362_03155"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNC73871.1"
                     /translation="MSIRVQSGQARGRRIKALPTGPDVRPILARIRKSLFDILRPRLA
                     GARFLDLFAGTGTVGIEALSNGAARAVFVDANRHSCRMVETNLAALGLSDRSKVIQSD
                     ATRLAVLASEGPFDLVFLGPPYKDDRRRPLALTVPALRALDEAKATTPEAWVIGQHHK
                     KEPLAGLPAGWEIFRENRYGDSVLTFFRRIPA"
     gene            2403..3155
                     /gene="argB"
                     /locus_tag="PK362_03160"
     CDS             2403..3155
                     /gene="argB"
                     /locus_tag="PK362_03160"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNC73872.1"
                     /translation="MTTTPSVIKFGGSLLSDAAARGAFLKDVAKFSKKESVVLVHGGG
                     PEINAALDKMGIKSAWVNGRRVTDEAAMAVVEGVLSGQVNKNLVGELNARGVAAVGLS
                     GRDAGLLTATPVPELGRVGEPAKVRPALLTALIKAKFLPVLSSVAQGPDGGALNVNAD
                     EAAAALAVAVKARRLVYLTDVPGVLDGQKKTLPEIRAKDVERLIADGVVTGGMIPKIR
                     SCARALKKGVKEINIIDGRAGLTAWAGTRLLP"
     gene            3268..>3961
                     /locus_tag="PK362_03165"
     CDS             3268..>3961
                     /locus_tag="PK362_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC73873.1"
                     /translation="MTGFVESPYLFNTYRRQPVTFVKGRGAWLTDDRGKKYLDFFSGL
                     AVCNLGHAHPAVARAVADQVKKLLHTSNIYSTQPQQALGKALVERTFPSKVFFSNSGA
                     EANELLIKLARRFGTVNPGPEGPRYEIIVFDSAFHGRTLGALSATPQKKYQEGFGPLL
                     PGFVVARYGDLSDVRQKATAKTCAVLVEPIQGEGGVRRVPPAFYSDLAALCREKNWLL
                     LFDEVQTGMGRTG"
BASE COUNT          749 a         1257 c         1203 g          752 t
ORIGIN      
        1 gtgatcggca gtgcccggcg cttcgccagc cacacctaca accggcggct ggaggacgtg
       61 gaccccttcc ccatattcgt cttcgacaaa ggcggcgtga ccgacgcggc ggagttcgtg
      121 gccacccacg agaaagcctt gaccggcatc gtgtcgggcg agcccaacta cgacgccctg
      181 tcggatttcg tattggccca ggaaaagatc cggaatttcg gttattacga aaacgagctc
      241 attttgatca agcgcttcgg cgccgtggtt tcctcggagg aatcgggcac catcgtcgag
      301 ctcatccggt tggcttattc cctttattgg agcctgcggg cctacaattt ctttttggaa
      361 aaggaaatcg accaggccca ggagttgttg gcggacctgc ccccctatta caagttttgg
      421 gccatgccct cccggtacca gacgttttcc cgggaagcca tggatttcgt ccgcgacaag
      481 ctggccatcg tggacagcct ccacaacgtg caggccaaca ttccccgcat cgactcggac
      541 tggcacctgc gcaccattta caaaaacgtc gaaaaggaat tcgacatcga cgagctttcc
      601 aaagccgtgg acacgaaact cgagcggatc gagcagtcgt acaactccgc ccgggatttc
      661 atttcaacca atttcttcat cgcggtggaa atcgtgttga ttctctccct ggcctggatg
      721 atgctggaca ccggccttct tttcatcatc gcccataaat aacgccgttt gacacccccc
      781 gacggggtgt ggtattctcc ccgccacggg tccgtccgga gcccccgccg cggttcgcgc
      841 ggggtcggag gcgggaccgc agattttcgg gaggaaaccg tacatgtccg atgcgtccgt
      901 ggaatacact ttggtcgtcg tcaaacccga cggccttaaa aaatctttga ccggcaacat
      961 tttgacgaaa ttggccgagg cccggttcat gatcatcggc gccaaggttc tccgggtgtc
     1021 ccgggagttg gccgaacaac attaccaaca cctgaagggc aagccgttct tcaacgattt
     1081 gatcgactac attcaaggaa agccctacgg gccggatttc gagcgcgtca tggccctcgt
     1141 gtacaagggc cccaacgcca tcaagaaaat ccgggaaatc gccggcgcca cgaaccccga
     1201 ggaagccgac tccgtctcca tccgcggggc tttcggccgg atcaccacca agggggtttt
     1261 tgagaacgtc attcacgcgt cgtccgacgc caaggattcc gagcgggaaa tcaagttgtg
     1321 gttccggccg gaagagatcg tcggcgaaat ctacccgacc aaaaaggttc aatcccaggc
     1381 ccacgaagtc gtcgcctggg cctaagccgg gcccgcggtg gatttttaag cgggaaaaga
     1441 gtaaaatagc tcttcaacgc cggccgatgg gcaccgcgcc cccggccgcg tttttttggg
     1501 ggcggaacga tgcgtccgct cgggttcatt cgatcaatat ttaaggagga cggcgccaac
     1561 gcagtgcttt tggtgccggt ggcggcccag agggccgacc cctccactgg ggctgtagct
     1621 cagctgggag agcgcctgca tggcatgcag gaggtcgtcg gttcgatccc gatcagctcc
     1681 acaattttga actgttgggg acacgccccg accctttggc ggcgccaaag ggccttcggc
     1741 agggcccgag gccccgaaac cccgaaggtc agctccacaa tttcatggtt tccttaaccc
     1801 cgccgttcgg cgttttccgc cgctaacatg tccattcgcg tccaatcggg ccaggcccgg
     1861 gggcgccgaa tcaaggccct tcccacgggc ccggatgtcc ggcccatcct cgcccgcata
     1921 cgaaaatccc ttttcgacat tcttcggccg cgcttggcgg gggcccgctt tttggacctc
     1981 ttcgccggca ccggcacggt cggcatcgaa gccctctcca acggggcggc ccgggccgtg
     2041 ttcgtggacg ccaatcggca ctcctgccgc atggtcgaaa ccaatttggc cgccctgggg
     2101 ttgtcggacc ggtccaaggt gattcaatcc gacgcgaccc ggttggcggt tctggcttcc
     2161 gaagggccct ttgacctggt gtttctgggc ccgccctaca aggacgatcg gcgaaggccc
     2221 ctggccctca ccgttccggc cctgcgggcc ctggacgagg cgaaagccac aacccccgaa
     2281 gcctgggtca tcggccagca ccacaaaaag gaaccgctgg cgggtcttcc ggcggggtgg
     2341 gaaatttttc gcgaaaaccg ctacggcgac agcgttctga ctttctttcg gaggattccc
     2401 gcgtgaccac caccccgtcc gtcatcaaat tcggcggcag ccttctttcg gacgccgcgg
     2461 cccgaggcgc tttcttaaag gacgtcgcca aattttccaa aaaggaatcc gtcgtcctcg
     2521 tccacggcgg cgggccggag attaacgcgg cgctggacaa aatgggaatc aaatccgcct
     2581 gggtcaacgg gcggcgcgtg acggacgaag cggccatggc ggtcgtggaa ggggtattgt
     2641 ccggtcaggt caataaaaat ctggtggggg aattgaacgc ccggggggtg gccgccgtgg
     2701 gtttgtccgg gcgggacgcc ggtcttttga ccgcgacccc cgtgccggaa ctgggacgcg
     2761 tgggcgagcc cgccaaggtc cgtcccgctt tgctcacggc gctgatcaaa gccaaatttt
     2821 tgccggtgct gtcctcggtg gcccagggcc cggacggcgg ggccttgaac gtcaacgccg
     2881 acgaggccgc ggccgcgctc gccgtcgcgg tgaaggcccg gcggctcgtt tacttgaccg
     2941 acgtgccggg cgttctggac ggccaaaaaa agacccttcc cgagattcgc gccaaggacg
     3001 tcgagcgcct catcgccgac ggcgtcgtga cgggcggcat gatccccaaa atccggtcct
     3061 gcgcccgggc gcttaaaaag ggcgtcaagg aaatcaacat catcgacggc cgggcggggc
     3121 tgaccgcctg ggccggcacg cggcttctgc catgacaggg gttcctccca tgccccgggc
     3181 tttggcccgg ggaaaaagcc cccagtgctt gcgcggggca aggaccccgc gcaagcggtg
     3241 gccgaccacg gagcgggagg ccatcccttg accggtttcg tcgaatcgcc ctatctcttt
     3301 aacacctacc ggcggcagcc ggtcaccttc gtcaaaggcc ggggcgcctg gctgaccgac
     3361 gaccggggca agaaatatct ggatttcttt tcgggtctcg cggtgtgcaa cctgggccac
     3421 gcccacccgg ccgtggcccg ggccgtggcg gaccaggtca aaaaacttct ccacacttcg
     3481 aacatttatt cgacccaacc ccaacaggcc ctgggcaagg ccctggtcga acggacgttc
     3541 ccgtccaagg tgttcttttc caattcgggg gccgaagcca acgaactatt gatcaaactc
     3601 gcccgccgtt tcgggaccgt gaacccgggc cccgaaggcc cgcgttacga aatcatcgtc
     3661 tttgatagcg cctttcacgg ccgcaccttg ggggcccttt ccgccacgcc ccagaagaaa
     3721 taccaggaag ggttcgggcc tttgttgccg gggttcgtgg tggcccgtta cggagacctg
     3781 tcggatgtcc ggcaaaaggc caccgccaag acctgcgccg ttctggtgga gcccattcag
     3841 ggggagggcg gcgtgcggcg ggtgccgccc gctttctatt ccgacctggc ggccctctgc
     3901 cgggaaaaaa attggctatt gctttttgac gaggttcaaa cgggaatggg acggacgggg
     3961 a
//
LOCUS       DAOQLS010000117         3960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11470_length_3960_cov_3.305506, whole genome shotgun sequence.
ACCESSION   DAOQLS010000117 DAOQLS010000000
VERSION     DAOQLS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11470_length_3960_cov_3.305506"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PK362_03170"
     CDS             <1..661
                     /locus_tag="PK362_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC73874.1"
                     /translation="IQAAGGPAPLILPGDIAQPGAAAARVEKTAAHFGRLDVLINNAG
                     ILRMSNFLQMPLSEMRDVFETNFWATVETVRAAVPILERQGGGRVVQIGSGVSRRGLP
                     FMGAYSASKFALLGLTESLRVELAAKGISFSLVLPGGTDTEMPANVDRSRLPPGYPHR
                     EGYRVSAARAARTVVKAVERGGAEYFVPWWVRPAAWVSALWPRFADFLVKKGYKTIQW
                     K"
     gene            652..1725
                     /gene="ychF"
                     /locus_tag="PK362_03175"
     CDS             652..1725
                     /gene="ychF"
                     /locus_tag="PK362_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNC73875.1"
                     /translation="MEIGIVGLPNVGKSTLFNALTGAQAAASNFPFTTIDPNVGVVPL
                     PDPRLATLGKMFASEKVTPAGIKFVDIAGLVKGASQGEGLGNKFLSHIRAVDAIAHVV
                     RCFHDPDVVNVLGKIDPGEAADIIETELLLSDLQQAQKALERLEGPARSGDKKARERL
                     DLMKSIVKGFEEGRSARSQNIPADLQLEQGFLTGKPVLFVANCDEKGPEPDLLAAIQA
                     RATRENAQVVALCAKMEAEIVQLPPEERATFYESAGITSPGLAELANAGKKLLKVMTY
                     FTAGPEESRAWLIPEGTRAQKAAGKIHSDIERGFIRAEKYRYDDLVRHGSVKALQEKG
                     LVSMEGKDYIMLDGDVVYFRFNV"
     gene            1816..2760
                     /locus_tag="PK362_03180"
     CDS             1816..2760
                     /locus_tag="PK362_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HNC73876.1"
                     /translation="MRHHRFRSIAGSLLSAALALAAGCRSKDSGLSRLTEGSPAGAPP
                     AAPAFTAVQNPANGHYYALFINDNAAPMLFEDAKDRAASVGGYLATITSEEENNFVRD
                     SFLVDGNIFTFLGALQAPGSQEPDGGWYWDNGEPWSYTNWSFGEPNNSSPLGYEDNVE
                     IYLFDDYRRGRWNDIPNTTQDLGGICNSYLVEWDADPNGDTEPPVIVALSASPAELWP
                     PNHTLRNVTVTAVLRDDRDPAPTYRIVDVSSNQPANGSGDGDASPDWILSGDHNVQLR
                     AERSGNLGDRVYTLTIEAADNAGNKATTAVTVKVPRSK"
     gene            2921..3208
                     /locus_tag="PK362_03185"
     CDS             2921..3208
                     /locus_tag="PK362_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73877.1"
                     /translation="MDETPNKANEARAHVAMLGRMTAWGGWILALGSLILGQYLLNKM
                     HQEIIGMQQENKNLQVSLSESRERLQKHLTEMVEKKAADKKRAASAKKAPR"
     gene            complement(3205..>3960)
                     /locus_tag="PK362_03190"
     CDS             complement(3205..>3960)
                     /locus_tag="PK362_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNC73878.1"
                     /translation="AGTIDKAGLEGFDAVVHLAGKSIAAGRWTRKLKDEILQSRVVGT
                     ELLAQSLAGLARPPKAFVCASAIGFYGSRGAEALGETSPAGSGYLPEVCKAWEAAAEP
                     ARQKGLRVAHVRFGVVLSDRGGALAKMLLPFKLGVGGVIGNGEQYMGWIALEDAVGAL
                     RHALDTPTLEGPLNAVAPQTLTNREFTKALGRALHRPTIFPLPGFAARLVLGGMAEAL
                     LLASQRVEPRRLLESGYRFRRPEIDGALKALLG"
BASE COUNT          778 a         1309 c         1220 g          653 t
ORIGIN      
        1 cattcaagcg gccggtggac cggcgcccct gattctcccc ggggacatcg cccaacccgg
       61 cgcggccgcg gctcgggtgg aaaaaacggc cgcccatttc gggcggttgg acgttctgat
      121 caacaacgcc ggcatcctcc gcatgtcgaa tttccttcaa atgcccctgt cggaaatgcg
      181 ggacgtcttc gaaaccaatt tttgggccac ggtggaaacg gtccgggcgg cggtgccgat
      241 cctggaacga cagggcggcg ggcgggtggt gcaaatcggc tccggcgtta gccggcgggg
      301 cctccccttc atgggggcct attcggccag caaattcgcc ctcctgggac tgacggaaag
      361 tttacgggtg gaactggcgg ccaagggaat ttccttcagc ctggtgttgc ccggcggcac
      421 cgacacggaa atgccggcca acgtggaccg aagccgactt ccccccggtt accctcaccg
      481 ggaaggctat cgggtctccg ccgcccgggc ggcccgaacg gtggtcaaag cggtggaacg
      541 gggcggggcc gaatatttcg tcccctggtg ggttcggccg gcggcctggg tgtcggccct
      601 ctggccccga ttcgcggatt ttctcgttaa gaaaggatac aagaccattc aatggaaata
      661 ggcatcgtcg gactccccaa cgtcggtaaa tcaacgctct tcaacgcgct gacgggcgcc
      721 caagcggccg ccagcaattt ccccttcacg accatcgacc cgaacgtcgg cgtcgtgccc
      781 ctgccggacc cccggctcgc cactctgggc aagatgttcg ccagcgaaaa ggtgaccccc
      841 gccggcatca aattcgtgga catcgcgggg ttggtcaagg gcgccagcca gggcgaaggt
      901 ctgggcaata agttcctttc ccacatacgc gccgtggacg ccatcgccca cgtggtgcgg
      961 tgttttcacg acccggacgt ggtgaacgtc ctggggaaaa tcgacccggg agaagcggcg
     1021 gacatcatcg aaaccgaact cctgctctcg gacctgcagc aggcccaaaa agccctggaa
     1081 cgcctggaag ggccggcccg ctccggcgac aaaaaagccc gggagcggtt ggacttgatg
     1141 aaatccatcg tgaaggggtt cgaggaaggc cgctcggccc gcagccaaaa cattcccgcg
     1201 gacctccaac tggagcaggg gtttttgacg ggaaaacccg ttctcttcgt ggccaactgc
     1261 gacgaaaagg gcccggagcc ggacctgctg gccgccatcc aggcccgggc cactcgggag
     1321 aacgcccagg tggtggccct ctgcgccaaa atggaggcgg agatcgtgca attgccgccg
     1381 gaggagcggg ccacctttta cgaatcggcg ggcatcacgt cccccggcct ggcggagctg
     1441 gccaacgcgg gaaagaagct tctcaaggtc atgacgtatt tcaccgcggg gcccgaggaa
     1501 tcccgggcct ggctcattcc cgaagggacc cgggcccaga aagccgccgg gaaaatccat
     1561 tccgacatcg aacggggctt tatccgggcg gaaaaatacc gctacgacga tctggtccgt
     1621 cacggatcgg tcaaggccct gcaggaaaag ggcctggtgt ccatggaagg caaggactac
     1681 atcatgctgg acggggacgt cgtctacttc cgttttaacg tttaattgcg cgctcttcgc
     1741 cttctttgtg tataatgccc acgtgggcat ttactaatgg aggcgtggag gcctccgctc
     1801 tcaggaggtt ttcccatgcg ccaccatcgt ttccgttcca tcgccgggtc gctgctgtcc
     1861 gcggccctgg ccctcgccgc cggttgccga agcaaagaca gcggcctctc ccgcctgacc
     1921 gaaggctctc ccgccggcgc gccgcccgcg gcgccggcct tcaccgccgt ccagaacccc
     1981 gccaacgggc actattacgc cctcttcatc aacgacaacg ccgcccccat gcttttcgaa
     2041 gacgcgaaag accgggcggc aagcgtcggg ggctacctgg ccaccatcac gagcgaagag
     2101 gaaaacaact tcgttcgcga cagctttctc gtggacggaa acattttcac gttcctgggc
     2161 gcccttcaag ccccgggttc ccaggagccc gacggcggat ggtactggga caacggcgaa
     2221 ccctggtcct acaccaactg gagcttcggg gaacccaaca attcctcccc cctgggctac
     2281 gaagacaacg tggagatcta cctgttcgat gattaccgtc gcgggcgctg gaacgacatc
     2341 ccgaacacca cccaggacct cggcgggatt tgcaattcct acctggtgga atgggacgcg
     2401 gaccccaacg gggacaccga accgccggtg atcgtcgccc tgagcgcctc ccccgctgag
     2461 ctctggcccc ccaaccacac cctgcgcaac gtgacggtga cggccgtcct tcgggacgac
     2521 cgggacccgg cccccaccta tcggatcgtc gacgtgagca gcaaccaacc ggcgaacggc
     2581 tccggggacg gagacgcctc gccggattgg attctctcgg gggaccacaa cgttcaactg
     2641 cgggccgaac gatccgggaa cctcggcgat cgcgtttaca cgctgaccat cgaagcggcc
     2701 gataacgcgg gcaacaaagc caccaccgcc gtgacggtca aagttccccg ctccaaataa
     2761 ttcgatccgc ctcgatcggc cggggcgtcg ctcttccttc gggaagaggc ggcgccccgg
     2821 ctgttttttt aagaacggga tgggggcttg tgccctccgg tcccatggtc tatattatgg
     2881 gggcgggacg ggtgcggatt tgacgattga aagaggtgtt atggacgaaa cgccgaacaa
     2941 agcgaacgaa gcccgggccc acgtggccat gctgggccgg atgaccgcct ggggcggttg
     3001 gatactggcg ctggggtcct tgatcctggg gcaatacctg ctcaataaaa tgcatcagga
     3061 aatcatcggc atgcagcagg aaaacaaaaa tcttcaggtc agcttgtccg aatcccggga
     3121 acggctacaa aagcacttga cggaaatggt ggaaaaaaag gccgccgata aaaaacgggc
     3181 ggcctccgcc aaaaaagcgc cgcgttagcc gagaagcgct ttcaaggcgc cgtcgatctc
     3241 cgggcgccgg aaccggtagc cggactccag caggcggcgg ggctccaccc gctggctggc
     3301 cagcaacaac gcctccgcca ttccccccaa caccagacgc gccgcgaaac ccggcaacgg
     3361 gaaaatcgtc ggccgatgaa gggcccgtcc cagggccttg gtgaattccc ggttggtcag
     3421 ggtctggggc gccacggcgt tcaaaggccc ttccaaggtc ggcgtgtcca gggcgtggcg
     3481 cagggcgccc accgcgtctt cgagggcgat ccaccccatg tattgctcgc cgttcccgat
     3541 gacccccccg acccccagct taaacggcaa caacattttg gccaaggcgc cgccgcgatc
     3601 gcttaacacc acgccgaaac gcacgtgggc cacccggagc cccttttgtc gggccggctc
     3661 ggccgccgct tcccaggcct tgcagacctc gggcaaatac cccgagccgg cgggactggt
     3721 ttcgcccagg gcttcggcgc cccgactccc gtaaaagccg atggccgagg cgcagacgaa
     3781 ggccttcggc gggcgggcaa ggcccgcgag ggattgagcc aaaagctccg tgccgaccac
     3841 ccggctttga aggatttcgt ccttcaattt ccgcgtccag cgcccggcgg cgatgctttt
     3901 tcccgccaga tgcacgacgg cgtcaaaccc ttccaagccc gctttatcga tggtcccggc
//
LOCUS       DAOQLS010000118         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11503_length_3954_cov_4.775327, whole genome shotgun sequence.
ACCESSION   DAOQLS010000118 DAOQLS010000000
VERSION     DAOQLS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11503_length_3954_cov_4.775327"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PK362_03195"
     CDS             <1..424
                     /locus_tag="PK362_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC73879.1"
                     /translation="VVLGLKLGADDYVTKPFSLREVLTRIKALLRRTAAPAAAAAPLA
                     VGALELDPERYELRLSKKPLSLTGKEFELLRTLWTAGGKALSREDLLERVWGLDRAMD
                     IDTRTVDQHVARLRSKLGAEKKRVRTVKNIGYRFLLDP"
     gene            421..1545
                     /locus_tag="PK362_03200"
     CDS             421..1545
                     /locus_tag="PK362_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC73880.1"
                     /translation="MSFWRRWKSAEKTPPRSAERPTPTTPAPDPFLASLLNALSQGVM
                     ALGEGGRVLLANEALSPVLNRPPSETIGLRLWEVLRQRDVADLADRGLAGRAEEREIL
                     FQTSPERLYRVRSHPFQHQRGPGLWLTFDDLTHARRLENIRKEFVANVSHELKTPLTA
                     LRAALETLIDGAVDDPDAAKDFLSTAQDQVDRLQRLIDDLLTLSRLEKPGAAAPSGAR
                     AEVAAIGRRVAKALEPLARKNRVSLSVDGVPPDLRAPLTEDELTQILMNLVDNAIKFN
                     RDNGRTILTATARDGEVVIEVKDTGVGIAREDQDRLFERFYRVDKARTRETGGTGLGL
                     SIVKHLVENRGGRVSVESTPGAGSCFKVVFPADSTDAARP"
     gene            1529..>3954
                     /gene="mgtA"
                     /locus_tag="PK362_03205"
     CDS             1529..>3954
                     /gene="mgtA"
                     /locus_tag="PK362_03205"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNC73881.1"
                     /translation="MPPDLKTDRGPLSPARWAACPLPDLFAEFKSTPAGLTDDEVVVR
                     RTAAGPNEPSRKRKRGLLLQLLSKLVDPLIVVLLVIAGASLFFGDRVSAAIVLGMSLI
                     SVGLSFFQEARAGRAAEKLSQVVRARATVLRGGRSLEVPFRDLVEGDVVDLYAGDMIP
                     ADLRVLQSKDLFLNQSALTGESLPVEKSPGAPTAPRPALSDLINIAFMGSSVVSGTGL
                     GLVVATGNRAQFGAVVRRLAERGEDSAFDRGVQRFVWFMVRAMLVLVSVIFLINLFAR
                     RNLPEAFFFALAVAVGLTPEMLPMLVTLNLSRGALALARKKVIVKRLNALQNLGAMDV
                     LCTDKTGTLTEDKVVLESHCDVVRREDESVLRMAYLNSHFQTGLKNLLDRAILSHTDI
                     SVEGIEKVDEIPFDFSRRLMSVVVTHGPRHRLLIKGAPEEIYKRCDFFELDGETLSMD
                     ALLLADLRREQDELSRAGFRVLAMAFRDLDPRPAYGKEDESHLTLKGYLAFLDPPKPS
                     AGEALRALAAQGVECKILTGDNSLVTETVCRQVGLEVRGTLSGTDLDDLTDDVLSEKL
                     ATVNVLSRLSPLQKERLVRLLKSRGRVVGFLGDGINDALALKAADVGISVDTAVDIAK
                     ESADLILLQKSLMILSDGVREGRRTFANILKYIRMGASSNFGNMLSMTGASLILPFLP
                     MLPLQVLVNNFLYDLSQLAIPTDRVDESQTTGPRPWDIARLRSFMFFFGPISSLFDFL
                     TFGVLLKIFHADAALFHTGWFMESIATQTLVIHVIRTEGRPWIDSRSSRPLLASSLGL
                     LGLALALPFSP"
BASE COUNT          690 a         1319 c         1197 g          748 t
ORIGIN      
        1 cgtggtgctc ggcctgaagc tgggcgcgga cgattacgtg acgaagccgt tttccctgcg
       61 cgaagtcctg acccggatca aggccctcct acgtcgaacc gccgctcccg cggccgcggc
      121 ggcgcccctg gcggtcggcg cccttgaact ggacccggaa cgctacgaat tgcgcctttc
      181 caaaaaaccc ctttctctga cgggcaagga gttcgaattg ctccggaccc tctggaccgc
      241 gggcggcaaa gccctttccc gggaagacct gctggaacgg gtttggggcc tggaccgggc
      301 catggacatc gacacccgca ccgtggatca acacgtggcc cggcttcgct ccaaactggg
      361 cgccgagaaa aagcgcgtgc gcaccgtcaa aaacatcggc taccggtttc tcctcgaccc
      421 gtgagctttt ggcgccgctg gaaatccgcc gagaaaacac cgccccggtc cgccgagaga
      481 ccgacgccga cgacccccgc cccggacccc ttcttggcct ccctgcttaa cgccctgtcc
      541 cagggggtga tggccctggg ggaaggcggg cgcgtgctcc tggccaacga agccctttct
      601 ccggtcctca accgcccccc atccgaaacc atcggcctgc gattgtggga agtccttcgg
      661 cagagggacg tcgccgacct ggccgaccgg ggactggccg gccgcgccga agaacgcgaa
      721 attcttttcc aaacctctcc cgaacgcctc taccgggtcc gctcccatcc ctttcaacac
      781 caaaggggac ccggcctttg gttgaccttc gacgacctca cccacgcccg gcggctggaa
      841 aacattcgga aggaattcgt cgccaacgtg tcccacgaac tcaaaacccc gctcacggcg
      901 ctccgggccg ccctggaaac gctgatcgac ggagccgtgg acgacccgga cgcggccaag
      961 gatttcctct cgaccgccca agaccaagtc gaccggctcc agcgcctgat cgacgacctc
     1021 ctgaccctct cccggttgga aaaaccgggg gcggcggccc cctccggagc ccgggccgag
     1081 gtggccgcga tcggccgccg ggtggccaag gccctggaac ccctggctcg aaaaaaccgc
     1141 gtttcccttt cggtcgacgg cgtgcccccg gatcttcggg ctcccctgac ggaggacgag
     1201 ttgacgcaaa tcctgatgaa cctcgtggac aacgccatca aattcaaccg cgacaacggc
     1261 cggacgattc tgacggcgac ggcgcgggac ggcgaagtgg tgatcgaggt caaagacacc
     1321 ggcgtgggca tcgcccggga agatcaagac cggctctttg agcggtttta ccgggtggac
     1381 aaggcgcgaa cccgggagac cggcggaacc ggcctcgggc tctccatcgt caagcatctc
     1441 gttgaaaacc gcggcggccg tgtgtcggtc gaaagcacgc cgggcgcggg cagttgcttc
     1501 aaagtcgttt tccccgcgga ttcgaccgat gccgcccgac cttaaaaccg accggggccc
     1561 gctctccccg gcccgctggg ccgcgtgccc gcttcccgat ctgttcgccg aattcaaatc
     1621 gacgccggcg gggcttacgg acgacgaggt cgtcgtccgt cgaacggccg cgggccccaa
     1681 cgagccgtct cgcaaacgca aacgcggcct cctgcttcaa ctcctgtcga aattggtcga
     1741 cccgctgatc gtcgttttgc ttgtgatcgc gggggcgtcg ctgttcttcg gcgaccgcgt
     1801 cagcgccgcc atcgttctcg ggatgtcctt gatcagcgtg gggctgtcgt ttttccagga
     1861 ggcccgggcc gggcgcgcgg cggaaaaatt gagccaggtc gtaagggccc gggccacggt
     1921 ccttcgtggc ggtcggtccc tggaagtccc tttccgggat ctggtcgaag gggatgtggt
     1981 cgacctttac gccggggaca tgatcccggc cgacctgcgc gttttacaat ccaaagattt
     2041 atttttgaac caatcggcat tgaccgggga gtccttgccg gtggaaaaat cccccggggc
     2101 gccgacggcg ccccggcccg ccctgtcgga tttaatcaac attgccttca tgggatcctc
     2161 ggtcgtcagc ggcacgggtt tgggcctggt ggtggccacg gggaatcggg cccaattcgg
     2221 cgcggtggtg cgccgactgg cggagcgcgg cgaggattcg gccttcgacc ggggcgttca
     2281 gcgcttcgtc tggttcatgg tgcgggccat gctcgttctg gtgagcgtga ttttcctgat
     2341 caacctcttc gcgcgccgca acctgcccga agcctttttc ttcgccctgg ccgtggccgt
     2401 cggcctcacc cccgaaatgc tgcccatgct cgtgacgctc aacctctccc ggggcgccct
     2461 ggctctggcc cgaaagaagg tgatcgtcaa gcgcttgaac gcccttcaaa acctgggggc
     2521 catggacgta ttgtgcaccg acaaaacggg caccttgacc gaagacaaag tcgttttgga
     2581 aagccattgc gacgtggtgc gccgggagga cgaatcggtc cttcgcatgg cttacctcaa
     2641 cagccatttt caaacgggct tgaaaaacct cctggaccgg gccatcctca gccacaccga
     2701 catttccgtc gaagggatcg aaaaggtcga tgaaattccc ttcgatttct cccgtcggct
     2761 catgtcggtg gtggtgaccc acggcccccg gcaccgactc ttaatcaagg gcgctcccga
     2821 ggagatctac aagcggtgcg atttttttga attggacggc gaaaccctct ccatggacgc
     2881 cctgctattg gccgaccttc gccgggaaca ggacgaactc agccgggccg gattccgggt
     2941 gttggccatg gcctttcggg acctggaccc ccgcccggcc tacgggaaag aagacgaatc
     3001 ccacctcacc ctgaaggggt atttggcttt tctggacccg cccaaaccct cggcgggcga
     3061 agccctgcgg gcgctggcgg cccagggggt ggaatgcaaa atcctcacgg gggacaattc
     3121 gctggtgacc gaaacggtgt gccgccaggt ggggttggaa gtccgcggca ccctctccgg
     3181 cacggacctg gacgatctga cggacgatgt cctttcggag aaactggcga cggtcaacgt
     3241 gctttcccgc ctgtcccccc tacaaaaaga gcggctcgtt cggttgctca agagccgggg
     3301 gcgggtggtg ggatttctgg gggacgggat caacgacgcc ctggccctca aagccgccga
     3361 cgtcggcatt tccgtggaca ccgcggtgga catcgccaag gagtccgccg atttgattct
     3421 gcttcaaaaa agcttgatga ttctttcgga cggggttcgg gaaggccggc ggacctttgc
     3481 caatattctc aaatacattc ggatgggcgc gagctccaac ttcgggaaca tgctgagcat
     3541 gaccggggcc agcctgattc ttcctttcct gcccatgctg ccccttcaag tgctcgtgaa
     3601 caactttctt tacgatctct cccagctggc catccccacc gaccgggtgg acgaatccca
     3661 aaccacgggg ccccgcccct gggacatcgc ccggttgcgc tccttcatgt tctttttcgg
     3721 gcccatcagt tcactctttg attttttgac tttcggggtc ctgctgaaaa tcttccatgc
     3781 cgacgcggcc ttgtttcaca cggggtggtt tatggagtcc atcgccaccc aaaccctggt
     3841 gatccacgtc atccgaacgg agggccgacc ctggatcgac agccggtcca gtcggccgct
     3901 gctcgcttca agcctcggcc ttttgggcct ggccctggcg cttccgttct cccc
//
LOCUS       DAOQLS010000119         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11539_length_3946_cov_4.389874, whole genome shotgun sequence.
ACCESSION   DAOQLS010000119 DAOQLS010000000
VERSION     DAOQLS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11539_length_3946_cov_4.389874"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1526)
                     /locus_tag="PK362_03210"
     CDS             complement(135..1526)
                     /locus_tag="PK362_03210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNC73882.1"
                     /translation="MNAILPELILAAAALTLLLLEAFRPGRKIPVYFLIVLGLAGAAW
                     ALGRSVDACVWDFLALDASTRYLKALALVAVALVSVYSATFPGFQREGFPWGTYFGLL
                     LLSTVGLFLMMSAKDLLLLLVAIELVSVTSFVLTGIVREDRRSSEAAIKYFLVGAFSA
                     GLMIYGFSILYGLLGTTRIDALLAPTVDLPALPLTGALFFVLAGFGFKLALAPFHMWA
                     PDVYEGAPTPVTAYLSVGPKAAAFGLLLHVLPNHGALRLTPLLAAISALTMTVGNLAA
                     LRQTNVKRLLAYSSVAQMGYVLMGFTAAGNNGARAVLIYLSGYVFMNLGAFASVLAMN
                     QASQTESIDGFKGLAQRSMPLALTTMFFMLSLTGIPPFVGFIGKFSLFSAVLQSTGLV
                     WLAVVAAVNSVISFAYYFSIVRAMFFETSDRPAPLSFSPALAGCLALTGVVTVAVGIF
                     PNTFLLLVQKVLP"
     gene            complement(1537..3021)
                     /locus_tag="PK362_03215"
     CDS             complement(1537..3021)
                     /locus_tag="PK362_03215"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNC73883.1"
                     /translation="MSLTLITFLPLIGSAVILLLPKEKKGLIRAFATAFAGAAFLVSI
                     HAYLNYNGGNAEMQLVERHAWIPVFDIHYYLGVDGLSMPLVLLTTLLSLISVIASYGI
                     ENRVKEYFFWFLMLETGMMGVFVSLDIFLFYVFWELTLVPMYFLIGVWGGPKKEYAAV
                     KFFLYTLAGSVFMLLGILALYFSSEPRTFDLLQLAQQSSKFMGVRFIWIFLALYVGFA
                     VKVPAFPFHTWLPLAHVEAPTAVSVILAGVLLKMGTYGLLRVSFAILPEATRWFMPIL
                     IVIAFINIVYGAFCAMNQRDIKRMVAYSSVNHMGYCLLGMAAITGSNASAGLSGAVLQ
                     MVNHGIITGSLFLLVGVIYDQAHTRNIDDFGGLGAKLPVYTGIMTVQVMASLGLPGLA
                     GFVSEFLCFLGGFGQRASLLWVSLSVIGIVVTAAFFLKLIKDVFLGPFNAKWEGHLKD
                     MSARELVTVVPLVVLTIFLGVYPTGLIQLMDATLTNLIRHVTGG"
     gene            complement(3051..>3946)
                     /locus_tag="PK362_03220"
     CDS             complement(3051..>3946)
                     /locus_tag="PK362_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNC73884.1"
                     /translation="EMPLMGGLGKKMPVTFITMSCAWLAIIGFPYISSGFYSKEAILA
                     AVYEKHQYVLFAIAGFTAFLTTFYMTRLMILTFIGTPRDAHRFQHAHESGPTMTLPLS
                     LLAVLSIVSGFLLHYIWPFAKLVPKAAEVVAEASTDAASEPGHLVVLGVSLAALALGL
                     IGAYAVYVLGSPNPKRLAERFPRIYTALGTRYFDAFYLKLVDVLCFKPAAFLAAFDYE
                     VVDQFFVDGVGTIGRFFSRVKSWIDDHIVDGVLVNGFGWVALKVGAGLRSVQTGVVQN
                     YLLAAALGVFALILWAVGAFG"
BASE COUNT         1007 a         1112 c         1237 g          590 t
ORIGIN      
        1 aaaccccgtg cgagaccggg cgaattcggc gaaagatttt tccaccgtgg aaaggaattc
       61 cgggaggcca tcggcccccg gggagcgaag aaccggttcc aacaccttga agacgggagg
      121 gaccacgaag gatcctaggg gagcactttc tgaacgagaa ggaggaaggt gttggggaag
      181 atcccgaccg ccactgttac cacccccgtc agggccaagc agccggccag ggccggggag
      241 aaggagaggg gcgccggtcg gtccgaggtc tcgaaaaaca tggcccgaac gatgctgaaa
      301 taataggcga aggagatcac gctgttgacg gcggccacca cggccaacca caccaagccc
      361 gtggattgaa ggacggccga aaagagcgaa aacttcccga taaatccaac gaagggcggt
      421 atgcccgtga gggacagcat gaaaaacatc gttgtcaagg ccagcggcat cgaacgttgg
      481 gccaacccct tgaagccgtc gatggactcc gtttgggaag cctggttcat cgccaaaacg
      541 ctcgcgaagg cccccaaatt catgaagacg tagccggaaa ggtaaatcag gacggcgcgg
      601 gcgccgttgt tgccggcggc ggtgaagccc atcagcacgt agcccatttg cgcgacggag
      661 gaataggcca ggagccgttt gacgttggtc tggcgcaggg cggccaaatt tcccaccgtc
      721 atcgtgagag cgctgatcgc ggccaaaaga ggggtcaaac gcaaggcgcc gtggttgggc
      781 agaacgtgaa gcagcaaccc gaaggcggcg gccttcggcc ccacggacag ataggccgtc
      841 accggcgtcg gcgccccttc gtaaacgtcg ggcgcccaca tgtggaaggg cgccagagcc
      901 aatttaaacc cgaacccggc caaaacgaag aacaaggccc cggtcaaggg aagcgccggc
      961 agatcgaccg tgggggccaa cagcgcgtcg atccgggtcg ttccgagaag gccgtagagg
     1021 atggaaaacc cgtaaatcat cagtccggcg gaaaaggccc cgaccaggaa atatttaatc
     1081 gccgcttcgc tcgaccggcg gtcctcccgg acgatgcccg tcagtacaaa ggacgtcacg
     1141 ctgaccaact cgattgccac gagcaggagc aggaggtctt tggcggacat catgaggaaa
     1201 aggccgacgg tggagagaag cagaaggccg aaataggttc cccagggaaa gccttcccgc
     1261 tggaatcccg gaaaagtcgc ggaataaacc gaaaccaggg ccacggccac cagggccagg
     1321 gcctttagat accgcgtcga ggcgtccagg gccaagaagt cccagacgca cgcgtccacc
     1381 gaacggccca gagcccaggc ggccccggcc aacccgagga cgatcaaaaa gtagacgggg
     1441 atttttcgcc cggggcggaa cgcttccaaa aggaggagcg tcagggccgc ggcggcgagg
     1501 atcagttcgg gcaggatcgc gttcatgccg gcctacttat ccccccgtca cgtgacggat
     1561 caaattcgtc aacgtggcgt ccatcagttg aatcaacccg gtgggataca cgccgaggaa
     1621 aatcgtgaga accaccaagg ggacgacggt gaccaattcc cgagcgctca tgtcctttaa
     1681 atggccctcc catttcgcgt tgaagggccc caggaaaacg tccttgatca atttaaggaa
     1741 aaaagccgcc gtcacaacga ttccaatgac cgacagcgaa acccaaagca gggacgcgcg
     1801 ttgaccgaac ccccccagga aacaaaggaa ttcggaaacg aatcccgcca gtccgggcag
     1861 gcccagggac gccatgactt gaaccgtcat gatccctgtg taaacgggga gcttggcgcc
     1921 caatccgcca aagtcgtcga tgttccgggt gtgcgcctgg tcgtaaatca cgccgaccaa
     1981 aaggaagagg gagccggtga tgatgccgtg gttcaccatt tggaggaccg ccccggacaa
     2041 accggccgag gcgttggacc cggtgatggc ggccatgccg aggaggcagt agcccatgtg
     2101 gttgaccgag gaataggcca ccatgcgctt gatgtcccgt tgattcatgg cgcagaaggc
     2161 gccgtagaca atgttgatga aggcgatgac gattaaaatc ggcatgaacc accgggtggc
     2221 ctcggggagg atggcgaagg acacgcgcaa cagaccgtag gtccccattt tgagcagcac
     2281 gccggccaat atgaccgaca ccgcggtggg cgcctccacg tgagccaggg gcaaccaggt
     2341 gtggaacggg aaggccggca ctttgacggc gaacccaacg tagagggcga ggaagatcca
     2401 aatgaaccgc acgcccatga atttgctgga ctgttgggcc aattgcagca ggtcaaaagt
     2461 gcggggttcg ctggagaaat agagagccag gatgcccaga agcatgaaca cgcttccggc
     2521 caaggtgtag aggaagaatt tgacggcggc gtattctttc ttgggtcccc cccaaacgcc
     2581 gatcaggaaa tacatgggga cgagggtcag ttcccagaaa acgtagaaaa gaaaaatgtc
     2641 cagagacacg aaaacgccca tcatgccggt ttccaacatc aggaaccaga agaaatattc
     2701 cttcacccgg ttttcaatcc cgtaggaggc gatgacggaa atcaaggaaa ggagcgtggt
     2761 caggagcacc aggggcatcg acaggccgtc cacgcccaag taatagtgga tgtcaaaaac
     2821 cggaatccag gcgtgccgtt ccaccaattg catttccgcg tttccgccgt tgtaattcag
     2881 gtaggcgtgg atggacacca ggaaggccgc gcccgcgaaa gcggtggcga aggcgcgaat
     2941 gagccccttt ttctccttcg gcaggagaag aatgaccgcg gacccgatca agggcagaaa
     3001 agtgattagc gtcaaagaca tggacaaggc tcctctagag ttctaagaaa tcaaccgaag
     3061 gcgccgacgg cccacaggat caaggcaaag acccccaggg ccgcggccag caaatagttt
     3121 tgaacgacgc cggtctgcac cgagcgcagg cccgccccga ctttcagtgc gacccacccg
     3181 aatccgttca ccagaacccc gtcgacgatg tggtcgtcga tccagctttt tacgcgggag
     3241 aaaaaccggc cgatggtccc aacgccgtcc acgaaaaact ggtccacgac ttcgtaatcg
     3301 aaggccgcga gaaaggcggc cggtttaaag cacaggacgt ccaccagttt caaataaaac
     3361 gcgtcgaagt agcgggttcc cagcgccgtg taaatccgcg gaaagcgctc ggccaatcgt
     3421 ttcgggttgg ggctgcccag aacgtatacc gcgtaggcgc cgatcaatcc caaggcgaga
     3481 gcggccaggg aaactcccag aaccaccagg tggccgggtt cgctcgccgc gtcggtcgac
     3541 gcttcggcca cgacctcggc cgccttggga acgagcttgg cgaagggcca aatgtagtgg
     3601 agcaggaagc ccgagacgat ggacagcacc gccaacaggg acaggggcag cgtcatggtc
     3661 gggccgcttt cgtgggcgtg ttggaagcgg tgggcgtccc ggggcgtgcc gatgaaagtc
     3721 agaatcatca accgcgtcat gtaaaaagtg gtcaggaaag cggtgaagcc ggcgatggcg
     3781 aaaaggacgt attgatgttt ctcgtatacg gccgccagga tggcctcctt cgaataaaac
     3841 ccggaggaaa tataagggaa cccgataatg gcgagccaag cgcaggacat ggtgatgaag
     3901 gtcaccggca tcttttttcc aaggccgccc atcaggggca tctcgt
//
LOCUS       DAOQLS010000120         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11671_length_3919_cov_4.883540, whole genome shotgun sequence.
ACCESSION   DAOQLS010000120 DAOQLS010000000
VERSION     DAOQLS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11671_length_3919_cov_4.883540"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..2143)
                     /locus_tag="PK362_03225"
     CDS             complement(92..2143)
                     /locus_tag="PK362_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73885.1"
                     /translation="MNRETGGRGAAPSMALSQLFGLSGLLWFLQFALLRWVGFEVPLL
                     AYHPHLMYLGFLFGLGAGCWRPATRRARAPLGPSIFLAAAALAAGRIPLAPAEGVEHF
                     WARGTAGMSGGIAVGVAVSLGLAFFLLAAWTAFPWGKILREGFAGPSLPAYGAYLGGG
                     LLGVLFSMAVNRWALPPWTALLSAGLAGLPFIGKGRPRAVHAGLAVLLGAMAAFSGRG
                     AIHSPYYRIDTAPLPVGRGLLLTANHSLHQAAIPMGPPAGTGKEAPAVARAREGFHRP
                     LAALRTVPKRALVLGAGLGNDVAALLVRGVPSIDAVEIDPVILAIGRSTHPDRPYQNP
                     GVRCFTEDARTFLRRSTASYDLIVMGTVDSMTRFSPFVNLRLENYLYTAESFTVLRHR
                     LAPGGGLIVYSFTPRPFVKMRLVALLARAFGELPVLLEGDYWVFNTALLAGPAFSHLR
                     SEGRGAFQAEFERDIAPNVRVPTDDRPYVFTRHPLGDPVWAATVTALLAAAAIGLIGA
                     RRGGGPFNGGEAALFGWSAAYMFLQSVSLARWALWSGSTWTTESLSLFFQSALALAAV
                     WTVRFRRVPRTLGPVGLLATLAVAVFVPPAGGASGGGLGLLAFAFYFLGIGVSVLFKD
                     AASASRALAVSLLGSLAGGALANGLGPLTGLRGLLCAAFVLYAATLLLVRRRGHDQNS
                     D"
     gene            2312..2890
                     /locus_tag="PK362_03230"
     CDS             2312..2890
                     /locus_tag="PK362_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73886.1"
                     /translation="MKKSLILAVSAALAAAPLVLKAEPRERRSEAQDFRAQRRAETQA
                     HYEQQRSENRAFQESLKDKTPEEKQAAVQAHRETQHRENTAFAAEQTEKRRVFLTQSL
                     AANTQLTDAQKADLIAFHENQWKENEATRETRYQESVAFFQKIAADNSLTQEQKKAAI
                     KAHRDEAKAAARAHLEQQKAENKAKRDSLKQP"
     gene            complement(2936..>3919)
                     /locus_tag="PK362_03235"
     CDS             complement(2936..>3919)
                     /locus_tag="PK362_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNC73887.1"
                     /translation="ARDILQQLSMVKPEGFKGSFFRSNLRLTCQKKGAGRDTKKDLLR
                     FVRARPGQCGIVYCLSRKTVESTAAFLNAHGVRAAPYHAGLSDEERRRNQNAFARDEI
                     DVVVATVAFGMGIDKSNVRYVVHRDMPKSLENYSQEIGRAGRDGLPADCVLFYSWADV
                     MAYERFAAPASDASLTAAAKQKTVEMFRWADRPVCRHRGLSAVFEEELGDCGNSCDVC
                     LKTTLDDLSPEDVRRSGTTERRLPARGRGIDVGSLDDPLFLRLKTLRRSLAEVQGVPA
                     YIVFSDAVLLRLAERRPRTLEEFRTVPGIGPAKEARYGEAFLRALNESPEE"
BASE COUNT          873 a         1337 c         1189 g          520 t
ORIGIN      
        1 gaacgccgat cgattgttcc gcacggcccc tcctccgcct aagtctatcc ccagaaacga
       61 ctcctttcaa acgaactttt acacttagaa atcaatccga attttgatcg tgtccccgcc
      121 gcctcaccag caggagggtg gcggcgtaaa ggacaaaagc cgcgcacagc agtcctcgga
      181 ggcccgtgag ggggcccaac ccgttggcca gggcgccgcc cgccagagaa cccaaaaggg
      241 aaaccgccag ggcccgggac gccgacgcgg cgtccttaaa aaggacggag accccgatgc
      301 ccaaaaaata aaaggcgaag gccagaagcc ccaggccccc ccccgacgcc ccgcccgccg
      361 gaggaacgaa gacggccacc gccagcgtcg ccaggaggcc cacgggcccc agcgtccggg
      421 gcacccggcg aaaacgaacc gtccaaaccg ccgcaagggc cagagccgat tgaaaaaaaa
      481 gggacaggct ttccgtcgtc caggtggacc cactccacag ggcccagcgg gccagggaaa
      541 cggactgaag aaacatgtag gccgcgctcc agccgaaaag cgccgcctcg ccaccgttga
      601 aggggccgcc gccccgccgg gctccgataa gaccgatggc cgccgcggcc agcagggccg
      661 tgaccgtggc cgcccacacg gggtccccca ggggatggcg ggtgaacacg tagggccggt
      721 cgtccgtggg aacccgaacg ttcggcgcga tgtcccgctc gaactccgct tgaaaagcgc
      781 cgcgcccctc ggaccgcaga tgggaaaagg cgggccccgc caagagcgcc gtgttgaaga
      841 cccaataatc tccttccaga aggacgggca gttccccgaa ggcccgtgcc aggagcgcca
      901 ccagccgcat cttcacgaag ggccggggcg taaaggaata aacgatcagc ccaccccccg
      961 gggccaaacg gtgccggagg accgtgaaag actcggcggt gtacaggtaa ttttccaggc
     1021 ggaggttcac gaaaggggag aaccgcgtca tggagtcaac ggtgcccatc acgatcaaat
     1081 cgtaggaggc ggtggatcgc cgcaggaacg tccgcgcgtc ctcggtgaag caacggaccc
     1141 ccggattctg gtaaggtcgg tccgggtgcg tcgaccggcc gatggccagg atcacgggat
     1201 cgatttccac cgcgtcaatc gacggaacgc cccggaccag cagggcggcc acatcgttcc
     1261 ccagccccgc cccgagaacc agagcccgct tgggcaccgt ccggagggcc gccagaggcc
     1321 ggtggaatcc ttcccgagcg cgggcaaccg cgggggcttc cttcccggtc ccggccggtg
     1381 ggcccatcgg gatcgccgcc tggtgcaggg aatggttcgc cgtcaggagc aggccccgcc
     1441 ccacgggcag gggggccgtg tcgatccggt aatagggact gtggatcgcc ccccggccgg
     1501 agaaagccgc catcgccccc aggaggacgg ccagcccggc gtgcaccgcc cggggtcgtc
     1561 cttttccgat gaaaggcaac cccgccagac ccgccgacaa gagggcggtc cagggcggca
     1621 aggcccagcg gttgaccgcc atggaaaaaa ggacccccag caggccgccc cccagatagg
     1681 ccccataggc cggaagcgac ggtcccgcaa aaccctccct taaaattttt ccccagggga
     1741 aagcggtcca ggccgccaga agaaaaaagg ccaggcccag ggacacggcg actccaacgg
     1801 cgatgccgcc ggacattccg gccgtgcccc gggcccagaa atgctcgacg ccttcggccg
     1861 gcgccagagg aatcctcccc gctgccaggg cggccgcggc caggaagatt gagggcccca
     1921 gcggcgcccg agcccgccgc gtcgccgggc gccagcatcc ggcgcccaga ccaaaaagga
     1981 atcccagata catcaggtgc gggtggtagg ccagcagagg gacttcaaac cccacccaac
     2041 gaagaagagc gaactgaaga aaccacagaa gcccggaaag accgaacaac tgggaaaggg
     2101 ccatcgaagg agccgcgccc cgcccgcccg tttccctatt catcgcccct cctttcgggg
     2161 tccgccaatc gccgaacggc tttcaatcat gccacctgtt tcaagggaaa acaaccggtt
     2221 atcccccgac acttttttcg cgccgtgtcg tccaaccaag tgaacgacaa cgccgcgtgt
     2281 tgatgcgcga tcgattcaaa aggagagtcc catgaaaaaa tcgttgatcc tcgccgtcag
     2341 cgcggccctg gccgccgccc ccctggtcct caaggccgaa cccagggaac gccgttccga
     2401 ggcccaggac tttcgcgccc agcgccgggc cgaaacccag gcccattacg aacagcaacg
     2461 gtccgagaac cgggcctttc aggaaagcct aaaagacaaa acccccgagg agaaacaggc
     2521 cgcggttcaa gcccaccggg aaacccagca ccgggaaaac acggccttcg ccgcggaaca
     2581 aacggaaaaa cgccgggtct ttctgaccca aagcctggcc gccaacaccc aactgaccga
     2641 cgcccagaag gccgacctca tcgcctttca cgaaaaccag tggaaggaga acgaggccac
     2701 ccgggaaacc cgctaccagg aaagcgtggc cttctttcaa aaaatagcgg ccgacaattc
     2761 cctgacccag gaacagaaaa aagccgccat caaagcccac cgggacgagg ccaaagccgc
     2821 cgcccgggcc cacttggaac agcaaaaggc ggaaaacaaa gccaaaaggg acagccttaa
     2881 acagccctaa aaagaaccgc cgccggggcc caccgtcgaa agacggtggg cccttttatt
     2941 cctccgggga ttcgttcaac gcccggagaa aggcctcgcc gtagcgggcc tctttggccg
     3001 gaccgatgcc gggcacggtt cgaaattctt cgagcgttcg gggccggcgc tcggccaatc
     3061 gcaaaaggac ggcgtcgctg aaaacgatgt aagcgggaac gccctggact tcggccaggg
     3121 accgtcgaag ggttttgagc cgcaaaaaca gggggtcgtc caaactcccc acgtcgatgc
     3181 cgcgcccacg ggccggtaaa cgccgctcgg tggttcccga gcgccggacg tcttccggcg
     3241 acagatcgtc cagggttgtt ttcaagcaga cgtcgcagga gtttccacag tcccccaatt
     3301 cctcttcaaa aaccgccgac aggccccggt ggcggcacac gggacggtcg gcccaccgaa
     3361 acatctcgac ggttttttgt ttcgcggcgg cggtcaacga ggcgtccgat gccggggcgg
     3421 caaatcgctc atacgccatc acgtccgccc aggaataaaa cagcacacag tcggcgggca
     3481 acccgtcccg ccccgcccgg ccgatttcct gggaataatt ttcgagactt ttcggcatgt
     3541 cgcggtggac cacgtaacgc acgttggatt tgtcgatgcc catgccaaag gccacggtgg
     3601 ccacgacgac gtcgatttcg tcccgggcga aggcgttttg attgcgccgc cgttcctcgt
     3661 cggaaagtcc ggcgtggtac ggcgccgccc gcaccccgtg ggcattcagg aacgccgccg
     3721 tggattccac cgttttgcgg ctcaaacaat aaacgatgcc gcactggccg gggcgcgccc
     3781 gcacaaatcg tagaaggtct tttttcgtgt cccggcccgc gccttttttc tggcaggtga
     3841 gccggaggtt cgaccggaag aaagatcctt tgaaaccttc gggtttcacc atgctcaatt
     3901 gttgaaggat gtcccgggc
//
LOCUS       DAOQLS010000121         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_11906_length_3878_cov_4.493330, whole genome shotgun sequence.
ACCESSION   DAOQLS010000121 DAOQLS010000000
VERSION     DAOQLS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11906_length_3878_cov_4.493330"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2538
                     /locus_tag="PK362_03240"
     CDS             <1..>2538
                     /locus_tag="PK362_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC73888.1"
                     /translation="AFNLSGGGQDGFALRRQAAALQREISAVPGVSETEIIGGRRRQL
                     LIHFDPAALARHRLTPLELAGTLEASNTRLPAGTQRAGDRSVAVETDALVRTAEDIKR
                     IVVGVSGGRPVTLADVARVVDGPDEEEADVAQWEKIGGEGPEGRPAVTVAVSKRRGEN
                     ATRVAEEVMKRIGDVRPTLLSTGTALDVTRNYGETAKEKSNELLFHMALATLSVTVLM
                     ALVLGVREALVVLIAIPVTLALTLLVYHLLGYTLNRITLFALIFSIGILVDDAIVVVE
                     NIHRHFTLNDGRSLWRLSVDAVAEVGNPTILATWAVIAAILPMAFVSGLMGPYMRPIP
                     VGASVAMLFSLGIAFVVSPWAFAHILEWWKPRPVAGHGAESRLDHFYRRMMGTLLSSV
                     RARWAYLLGTAVLLAAAASLVYFKAVTVKMLPFDNKNEFEVVLSLPEGTALSQTKRAT
                     DEIARALLESPEVERVTNYVGTAAPYNFNGLVRHYFLRQRPHQADLAVNLTDKKTRHV
                     QSHGIATRLRPGIQAIADRYGARVQVAEIPPGPPVLSTLVFELYGPDNARRDALARKL
                     EQWLKTAPGVVDVDSYVPAPEPLDRLVVDREKASLNGLPPAAVAQTVSLSLGGQTLGL
                     AHTEDREPVEIRLRLSPENRRGLDALRGLQMMSRNGTMLSLGTLTRTERTERDAPVYH
                     KNLQRASYVIADVAGKQESPVYAILALRRGIREMAEKEGFALREYFSKQPGNSQEWAL
                     KWDGEWQITYEVFRDLGLAFAFALLLIYVLVVGWFRSFLIPLVVMVPIPLTLVGILPA
                     HWALGAYFTATSMIGFIAGAGIVVRNSIILVDFIHLRLAEGMPLKEAV"
     assembly_gap    2539..2638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2639..2904
                     /locus_tag="PK362_03245"
     CDS             <2639..2904
                     /locus_tag="PK362_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC73889.1"
                     /translation="VDFIHLRLAEGMPLKEAVIDAGAVRFRPMLLTAAAVVVGAGVIL
                     FDPIFQGLAVALMAGEGASTLLSRVAVPVLYYMVARRSPAAGK"
     gene            complement(2959..>3878)
                     /locus_tag="PK362_03250"
     CDS             complement(2959..>3878)
                     /locus_tag="PK362_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC73890.1"
                     /translation="LYSIVAIPLMYSFGMMYRPGTNFAVADFWRWWIVHLWVEGFFEL
                     FTTIVVAHIFVLLGLVSREAALRVVYLDIVLYLGSGMIGTAHHYYWTAQPAINLALGA
                     IFSAMEVVPLCLLTLEAWDFIKLRQDKSVLGVDPKAFPHKWTVMFLMAVGFWNFLGAG
                     VFGFLINLPIVSYYEHATYLTSNHGHAALMGVYGNLAIAALAFCTRVLVRPERWNDRL
                     WGVSFWSLNAGLMLMLLLNIFPAGIYQLVQSVNHGFWYARSAAVLNSSFFQTATWARV
                     VGDLVFVVGGVVPIAYFMVTRLFSLRSAK"
BASE COUNT          722 a         1196 c         1177 g          683 t
ORIGIN      
        1 ggctttcaac ctctccggcg gggggcagga cggcttcgcc cttcggcgcc aggccgccgc
       61 ccttcagcgg gaaatcagcg cggtgccggg cgtttccgaa acggaaatca tcggcggccg
      121 ccggcggcaa ttgttgattc atttcgatcc cgccgccctg gcccggcacc ggttgacgcc
      181 cctggagttg gcgggaaccc tcgaggcctc caacacgcgc cttcccgccg ggacccaacg
      241 cgcgggcgac cgctccgtgg cggtggaaac cgacgcgttg gtccgcaccg cggaggatat
      301 aaagagaatc gtcgtgggcg tctccggcgg ccgcccggtc actctggccg acgtggcccg
      361 ggtggtcgac ggccccgatg aagaagaggc cgatgtcgcc cagtgggaaa aaatcggggg
      421 cgagggcccg gaaggacggc ccgccgtgac ggtggcggtt tccaagcgcc ggggggaaaa
      481 cgccacccgg gtggccgagg aagtgatgaa gcggatcggg gacgttcgcc cgaccctgct
      541 ttccaccggc accgccctgg acgtcacccg gaactacggg gaaaccgcca aggaaaaatc
      601 caacgagctc ctattccaca tggccctggc caccctttcc gtcacggtgc tgatggcctt
      661 ggtcctgggg gtgcgggaag ccctggtggt gttgatcgcc atcccggtga ccctggccct
      721 gacgctcctg gtttatcatc tgttgggata cacgctcaac cgcatcaccc tgttcgccct
      781 catcttttcc atcggaatcc tcgtggacga cgccatcgtg gtggtggaaa acatccaccg
      841 ccatttcacc ctgaacgacg gtcgttccct ctggcgactt tcggtggacg ccgtggccga
      901 agtgggcaat cccaccatcc tggccacctg ggccgtcatc gcggccatcc tccccatggc
      961 cttcgtgtcg ggtcttatgg ggccctacat gcgccccatc ccggtgggcg ccagcgtggc
     1021 catgttgttt tccctcggca tcgccttcgt ggtcagccct tgggctttcg cgcatattct
     1081 ggaatggtgg aaaccccgtc ccgtcgcggg acacggcgcg gaatcccgct tggaccactt
     1141 ctaccggcgg atgatgggaa cccttctgtc ctcggtccgg gcccggtggg cctacttgtt
     1201 gggcaccgcg gtgttgctgg cggccgccgc gtccttggtt tacttcaagg ccgtcaccgt
     1261 taaaatgttg cccttcgaca acaaaaacga atttgaagtc gtcctgagcc tcccggaggg
     1321 caccgccctt tcccaaacga agcgcgcaac ggacgaaatt gcccgggccc tgctggaatc
     1381 ccccgaggtg gaacgcgtga ccaattacgt gggaacggcc gctccgtaca acttcaacgg
     1441 actggtccgg cattatttcc ttcgtcagcg cccccatcag gcggatttgg cggtgaacct
     1501 cacggacaag aaaacgcggc acgtccaaag ccacggcatc gccacgcggc tccgtcccgg
     1561 aatccaggcc atcgccgacc gctacggcgc ccgggtccaa gtggccgaaa tccccccggg
     1621 gccgccggtc ctctcgaccc tggtctttga gctttacgga cccgacaacg cccgtcggga
     1681 cgccttggcc cggaaattgg agcaatggct taaaaccgcg ccgggggtcg tggacgtgga
     1741 cagctatgtc cccgccccgg aacccctgga ccggctggtg gtggaccggg aaaaagcttc
     1801 gctcaacggc ttgccgcccg ccgcggtcgc gcaaacggtg tcgttgtccc tcggcgggca
     1861 aaccttgggt ctggcccaca ccgaggaccg ggaaccggtc gaaatacgcc tgcgtctttc
     1921 cccggaaaat cgacgggggt tggacgccct gcgcggtctc caaatgatgt cccgcaacgg
     1981 gacgatgctt tccctgggaa ccttgacccg tacggaacga acggaacggg acgcgccggt
     2041 gtaccacaaa aatcttcagc gggcgagcta cgtcatcgcg gatgtcgcgg gcaaacagga
     2101 aagccccgtc tacgccattc tggccctccg ccgaggaatt cgcgaaatgg cggaaaaaga
     2161 aggatttgcc ctccgggaat atttttcaaa acagcccgga aattcccagg agtgggcact
     2221 gaaatgggac ggggaatggc aaataaccta cgaggtcttt cgggacctgg ggctggcctt
     2281 tgccttcgcc ttgctgttga tttatgtgtt ggtggtgggg tggttccgtt cgttcctgat
     2341 tccgctggtg gtgatggtgc cgatcccgtt gaccctggtg ggcattctac cggcccattg
     2401 ggctctgggg gcctatttca cggccaccag catgattggg ttcatcgccg gggcggggat
     2461 cgtggtccgc aattccatca tcttggtgga ttttattcat ctgcgcctgg ccgaagggat
     2521 gccattgaaa gaggccgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     2641 gtggatttta ttcatctgcg cctggccgaa gggatgccat tgaaagaggc cgtcatcgac
     2701 gcgggcgccg tgcgtttccg gcccatgctg ctcaccgccg cggcggtggt ggtgggcgcc
     2761 ggggtgatcc tgtttgaccc catctttcag ggcctcgccg tggccctcat ggcgggcgag
     2821 ggggcctcga ccctcctttc ccgggtggcc gtgcccgtcc tttattacat ggtcgcccgt
     2881 cgctctcccg ccgccggaaa ataaaacgcc cccgcccctt ccaagatcga aggagcgggg
     2941 gcgtttcgaa cggagtgtct atttcgcgga ccgaagggag aagagccgcg tcaccataaa
     3001 ataggcgatg ggaacgacgc cgcccaccac aaacaccagg tcccccacga cccgggccca
     3061 agtcgcggtt tgaaagaagg acgagttcaa gaccgccgcg cttcgggcgt accaaaaccc
     3121 gtgattaacg ctctgaacca actggtaaat ccccgccggg aaaatgttga gcaggagcat
     3181 caacatcaag cccgcattca gggaccagaa ggaaacgccc cacagccggt cgttccaacg
     3241 ctccggccgg acgaggaccc gggtgcagaa ggccagggcg gcgatcgcca ggttgccgta
     3301 gacgcccatc agtgccgcgt ggccgtgatt ggacgtcagg taggtcgcgt gctcgtagta
     3361 gctcacgatg ggcaggttga tcaggaatcc gaagaccccc gcgcccagga agttccaaaa
     3421 acccacggcc attaaaaaca tgaccgtcca tttgtgggga aaagccttgg ggtccacgcc
     3481 cagaacgctc ttgtcctggc gcagtttgat gaaatcccag gcttccagcg tcagcaggca
     3541 gagcggaacc acttccatgg cggaaaagat ggcgcccagg gcgaggttga tcgccggttg
     3601 cgccgtccag tagtagtggt gggcggtgcc gatcatgccg ctccccagat aaagaacgat
     3661 gtccaggtaa acgacccgca gggccgcttc ccgggacacc aagcccaaaa gaacgaagat
     3721 gtgggccacg acgatggtcg tgaacagctc aaaaaaaccc tccacccaaa gatgcacgat
     3781 ccaccagcgc cagaaatccg ccacggcgaa gttggtgccg gggcggtaca tcatgccgaa
     3841 ggaatacatg agcggaatgg ccacgatgga ataaagga
//
LOCUS       DAOQLS010000122         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12006_length_3861_cov_4.016816, whole genome shotgun sequence.
ACCESSION   DAOQLS010000122 DAOQLS010000000
VERSION     DAOQLS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12006_length_3861_cov_4.016816"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..2451
                     /locus_tag="PK362_03255"
     CDS             223..2451
                     /locus_tag="PK362_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC73891.1"
                     /translation="MNSRLHGLLKKQYARFFKDRGGPFLWRFRSFLKAVNEAYHQDEQ
                     DRKNLEHTLDLRGQEIARRNTHLQAIFQTFPDLFLVINEQEEIVEFSGASFDDNAESG
                     RWIGRPLESFPNPDVGRRFRDSLRQARAARAPTTLEYALLAGSSLGFYEARLRPMDDG
                     QAIILIRDITGRKAALEALKASEERYALAARAANDGLWDWNLPNNRIYFSPRWKALLG
                     YSDAELGNAPSVWLDRVHPDDVEMVKRTLSETGQPGKDSLEIEYRIRHRDNTYHWALT
                     RGLVVRDDAGAAYRLVGSQTDITTRKSAEEQLRHEAFHDKVTGLANRALFLDRLTNTL
                     DRVKRQPGYLFAVLYVDLDRFSTINERAGHEAADRLLQAVAARLRSYARVGDTVARLG
                     DDEFAVLLDGIANERSAVVFADRMRELIGAPCDIGPEVVSVGASLGIALNGPESVNAE
                     MLLNEAESAMQRAKKNGKNRHEIFDRETHSRLVSKLKIESDLRQAAERGELELYYQPI
                     FSLKDAEIIRVEALIRWNHPRRGQVSPVEFIPVAEETGLILNIGDWVLQEVADQTRRW
                     HAEGFAPIAVAVNFSPKQFQQQNLLTTVGTILSRCKHTGFPLEIEITESAAMQDVDET
                     IKILKNLREMGVRISMDDFGVGYSSLSCLRLFPLNTLKIDRGFVQGIPALKDNTAITN
                     AIIGIGHSLGLSLVAEGVENEEQLNFLKSQGVEEGQGFLLGKPMPARDLAPLLRRNGK
                     PA"
     gene            2527..3741
                     /locus_tag="PK362_03260"
     CDS             2527..3741
                     /locus_tag="PK362_03260"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNC73892.1"
                     /translation="MSRGYAYIVLGAGKQGMAAAFELALFGRASTVTVADASLPLARA
                     AVDRLQSLIGKELKQNGTTLRSRRVDGRRATDLAKAIDGHRGAFSALPYFLNPAAARA
                     AIASGAHYVDLGGHFDTTQEILKLERGAQKSGVTVTPDCGVAPGLCNVLAARGIEQLD
                     RADEVRMYCGGLPQHPHPPLGYKLVFSMEGVLGNYFGKSYELREGRVVLVPSFERREE
                     LDFGPPLGRLEGVVTGGATSTCPWTYEGRVQSYSYKTLRYPGHFEKIQALKDLGLLDA
                     EPIRVDGQWVSPRRLFIKLAEERLRFPGERDLLVMRVTVRGTKGGAPTEIAYNLLEYE
                     DVKTGFSAMQRTTGFPAAIVLEMLVQGEVAKPGVVPIEKLVPPKTYLDLVQRRGIKIT
                     ETVAAPKTGPAS"
BASE COUNT          748 a         1331 c         1156 g          626 t
ORIGIN      
        1 ccgggccagg gccgggtcgc ggttctccgc caccgcgccc cttcgcggct tcgtttgtag
       61 caccgccaat cgcgtcagac cgatcttcgc caccgacccc tccgggggga taaagacccc
      121 ttttgcccct tgattatagg tgaaacgggg gctaagtata taaggcctcc cggacgcgac
      181 ggccggcacg ggccacggga cgcagggacg aaaggaggtc ggatgaattc acgtcttcat
      241 ggtctcctta aaaaacaata cgcccgattt tttaaagacc gcgggggacc cttcctctgg
      301 cggttccgct cgtttttgaa agccgtcaac gaagcctacc accaagacga acaagaccga
      361 aagaatctcg aacacacgct cgacctgcgc ggccaggaaa tcgcccgtcg gaacacccat
      421 ttgcaggcca tcttccaaac gttccccgac ctcttcctgg tgatcaacga gcaggaggaa
      481 atcgtcgagt tcagcggggc ttccttcgac gacaacgcgg aatccggccg ctggatcgga
      541 cgcccccttg aatcgttccc caacccggac gtgggccgtc ggttccggga ttcccttcgc
      601 caggcccgag ccgcccgggc cccgaccacc ctggaatacg ccctgctggc cggctcctcc
      661 ctgggttttt acgaagcgcg cctccgcccc atggacgacg ggcaggccat catcctcatc
      721 cgggacatca cgggccgaaa ggccgctctg gaagccctca aggcgtccga ggagcgatac
      781 gccctggccg cccgggccgc caacgacggg ctctgggact ggaacctgcc caacaaccgg
      841 atctactttt cgccccgctg gaaagccctg ctcggctact ccgacgccga actgggcaac
      901 gccccgagcg tctggctcga ccgggtccac cccgacgacg tcgaaatggt gaaacgcacc
      961 ctctccgaaa cggggcagcc gggcaaggat tccctggaaa tcgaataccg catccgccac
     1021 cgggacaaca cctaccattg ggccctgacc cgggggctgg tcgtgcggga cgacgccggc
     1081 gccgcctacc gcctggtggg ctcccagacg gacatcacga cgcggaaatc cgcggaggaa
     1141 cagctccgcc acgaggcgtt ccacgacaaa gtcacgggcc tggccaaccg ggccctcttc
     1201 ctggaccgcc tgacgaacac cctggaccgc gtgaagcggc agcccggtta tttgttcgcc
     1261 gttttgtacg tggatttgga ccggttcagc acgatcaacg aacgcgccgg ccacgaggcg
     1321 gccgaccgtt tgctgcaggc cgtcgccgcc cggctccggt cttacgcccg cgtcggcgac
     1381 acggtggcgc ggctggggga cgacgaattc gccgtcctcc tggacggcat cgccaacgaa
     1441 cgctccgccg tggtcttcgc cgaccgcatg cgcgagctga tcggggcgcc ctgcgacatc
     1501 ggccccgaag tggtcagcgt cggagcgagc ctcggcatcg ccttgaacgg gcccgagagc
     1561 gtcaacgccg aaatgctgct caacgaagcc gaaagcgcca tgcagcgggc caagaaaaac
     1621 ggcaaaaacc gccacgagat tttcgaccgg gaaacccaca gccgactggt ctccaaactg
     1681 aaaatcgaat cggacctgcg ccaggccgcc gagcgcgggg aactggagct ctattaccaa
     1741 cccatctttt ccttaaagga cgccgagatc atccgggtcg aggccctgat ccgctggaac
     1801 cacccccggc gcggccaggt gtcccccgtg gaattcatcc ccgtggcgga ggaaacgggc
     1861 ctgatcctca acatcggcga ttgggtcctc caggaagtgg ccgaccagac gcgccgctgg
     1921 cacgccgaag gcttcgcccc catcgcggtg gcggtcaatt tttcgcccaa acaattccaa
     1981 caacagaatt tgctgaccac ggtggggacg attctgagcc ggtgcaagca cacgggcttt
     2041 cccttggaaa tcgagatcac cgaaagcgcc gccatgcagg acgtcgacga aacgatcaag
     2101 attttaaaga acctccggga gatgggcgtc cgcatatcca tggacgattt cggggtcggg
     2161 tattcctccc tgagttgcct ccgcctcttc cccctgaaca ccctcaaaat cgaccggggg
     2221 ttcgtccagg gcattccggc cctcaaggac aacaccgcca tcaccaacgc catcatcggc
     2281 atcggccaca gtttgggact gtccctggtg gccgaaggcg tcgaaaacga ggaacagctc
     2341 aacttcctga aatcccaggg cgtggaggaa ggccaagggt tcctgctggg gaaacccatg
     2401 cccgcccggg atctcgcccc cctcctccgc cgcaacggca aacccgccta attttcgtcc
     2461 cctccgtcgg taaaaggaaa cccgcccgac cttgaatttg gcccgtcccg gggctaccct
     2521 aacggggtga gtcgaggcta cgcatacatc gtgctcggcg ccggcaaaca aggcatggcc
     2581 gcggccttcg aactggccct ctttgggcgg gcttccacgg tcaccgtggc cgacgcgtcc
     2641 cttcccctgg cccgcgccgc ggtggatcgc cttcaatcct tgatcggcaa agagctcaaa
     2701 caaaacggca cgacgctccg ctcccggcgc gtggacggcc ggcgagccac cgatttggcc
     2761 aaggccatcg acggccaccg gggggccttc agcgccctcc cctatttctt gaaccccgcc
     2821 gccgcccggg ccgccatcgc ttcgggggcc cactacgtgg atttgggcgg tcattttgac
     2881 accacccagg aaattctgaa attggagcgg ggcgcccaaa aatccggggt gaccgtcacg
     2941 cccgattgcg gcgtcgcccc cggcctctgc aacgtcctgg ccgcccgagg catcgagcag
     3001 ctggaccgcg ccgacgaggt ccgaatgtac tgcggcggcc tcccccaaca tccccacccc
     3061 cccctgggct acaaattggt gttcagcatg gaaggggttc tcggcaatta tttcggaaag
     3121 tcctacgaac tgcgggaagg gcgggtggtc ctggtgccgt cctttgaacg acgggaggag
     3181 ctggatttcg gtccacccct gggtcggttg gagggcgtgg tcacgggcgg cgccacgtcg
     3241 acctgtccct ggacctacga aggacgtgtg caatcctaca gctacaaaac cctgcgctac
     3301 cccggccact ttgaaaaaat ccaggcgttg aaggatctgg ggcttctgga cgccgagccg
     3361 atccgggtgg acggccaatg ggtgtcgccc cggcgccttt tcatcaagtt ggcggaggag
     3421 cggctgcggt ttcccggcga gcgggacctg ctggtcatgc gggtgacggt gcgggggacg
     3481 aagggcgggg ccccgaccga aatcgcctac aacttgttgg aatacgagga cgtcaagacg
     3541 ggcttttcgg ccatgcagcg caccacgggg tttcccgcgg ccatcgtgct ggaaatgctg
     3601 gtgcagggcg aggtggccaa gccgggcgtc gttcccattg aaaaattggt cccgcccaaa
     3661 acctatttgg acctcgtcca acgccgcggc atcaaaatca ccgaaaccgt cgcggccccc
     3721 aaaaccggac cggcgtccta accgggccgg ccctcccgca aggaccgaaa cgcccgggcc
     3781 accgcggccc acacccgatc ggggttctcc gacagcacgg gatcgagcag cgcccccccg
     3841 tcgtaactcc acgccgtgat g
//
LOCUS       DAOQLS010000123         3829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12165_length_3829_cov_3.664812, whole genome shotgun sequence.
ACCESSION   DAOQLS010000123 DAOQLS010000000
VERSION     DAOQLS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12165_length_3829_cov_3.664812"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK362_03265"
     CDS             <1..547
                     /locus_tag="PK362_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73893.1"
                     /translation="LWVLRFEAAELNRRTVKAIDVSPLFNDARASERTATLRALELAR
                     RVPAGGVTHLLVDWDGLRSAPEEADGAIVRKAEARLNQEVPGLLRLDNRVALRLWVRG
                     TSAGQKVFRPEGISVRALRRFDGAAAQPLTLLGVALDRIVPAERASLAEWLVSLVPLE
                     GLVSADVEAALRRLVFLQIQA"
     gene            617..1015
                     /gene="sdhC"
                     /locus_tag="PK362_03270"
     CDS             617..1015
                     /gene="sdhC"
                     /locus_tag="PK362_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNC73894.1"
                     /translation="MKRLLEETSDIVRYRGGFGHWTFLLHRLTGLGVLVFLLAHVVDT
                     ALLGWGPDVFNRVMAVYRHPFFRLSEVFLVASVLFHTLNGVRLIVVDFWPRATRHHRA
                     LVRGTWVLFVLLMIPVVIIMAHHYLEANPR"
     gene            1012..1443
                     /locus_tag="PK362_03275"
     CDS             1012..1443
                     /locus_tag="PK362_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HNC73895.1"
                     /translation="MTTLSSNAGRPLPAGGFERFAWFFMRASGLVLLVLALGHLAIMH
                     LINSIDTVDYAFVAARYATPFWRTYDGLMLVLALLHGYNGLRVVLNDYLKSGWRTAAT
                     WFMGLLTLVLLILGLYVVFAFQPDLPDNTVLPNDTDLTSSL"
     gene            1415..3157
                     /gene="sdhA"
                     /locus_tag="PK362_03280"
     CDS             1415..3157
                     /gene="sdhA"
                     /locus_tag="PK362_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNC73896.1"
                     /translation="MTRTSHHRFDCLIVGAGGAGLYAALALAQRKGSSVAVLSKLYPT
                     RSHTGAAQGGVSAALGNTEDDSPDWHMFDTVKGGDYLVDQDAAEILCRDAVSTVIELE
                     HLGLPFNRTEDGRIDQRRFGGHTREFGKGPVRRACYAADRTGHMILQTLYQQCIKNNV
                     TFFDEYHVVDLILSAGRCAGVVAYQISTGELHVFHAKSVLFATGGSGRMFKITSNAHA
                     LTGDGMAIAYRRGLPLEDMEFFQFHPTGIYKMGILLSEACRGEGGKLLNGKGERFMER
                     YAPTMLDLAPRDMISRFIHQEIREGRGVDGKDYVHLDLRHLGAQVIEEKLPDITDFVR
                     TYMGLDPVTDLIPIQATAHYAMGGIPTTVDGEVIVDAKNTVVPGFYAAGEVACVSVHG
                     ANRLGTNSLVDILVFGRRSGLAMAEYAAKNDFTPLPADPAADVRERVARLLANPPKQS
                     AAALRAELQAAMMDDCGVYRSEAGLKKVQAKIVECRARYAGVGVADKGKQFNTELLEA
                     LELENLIDLAECTVASAIARKESRGAHAREDFPKRDDAQFLKHTLATKTPTGVSLDYK
                     PVVITKFQPQERKY"
     gene            3157..>3829
                     /locus_tag="PK362_03285"
     CDS             3157..>3829
                     /locus_tag="PK362_03285"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNC73897.1"
                     /translation="MKVTLKIRRVDPEKGLPERWETYTVDVAPTDRVLDALNEVKWTQ
                     EGPLAYRRSCAHGICGSDAMRINGRNRLACKVLVQDVAPVITLEPMKGFRVIKDLIVD
                     MEPFLAKFRSLKPFLINDEPVTEGERLQSPAARARFDDTTKCILCGACTTSCPSFWAD
                     PAYVGPAAIVNAHRFIFDDRDRAAAERLELLDGRDGVWKCRTVFNCVEACPRDIDITG
                     AIADVK"
BASE COUNT          647 a         1298 c         1150 g          734 t
ORIGIN      
        1 cctgtgggtt cttcggttcg aggccgctga attgaaccgc cgaacggtca aggccatcga
       61 cgtgtcgccc ctctttaacg acgcgcgcgc ttccgagcga acggcgactc tccgcgcctt
      121 ggagctggcc cgtcgggtcc ccgccggggg cgtcacccat ttgctggtgg attgggacgg
      181 gctccggtcc gcgccggagg aagccgacgg ggcgatcgtt cggaaagccg aggcccggtt
      241 gaaccaagaa gtcccggggt tgctccggct cgacaaccgc gtcgccctcc gactgtgggt
      301 tcggggaacc tccgccggtc aaaaagtttt ccggcccgag gggatttcgg ttcgggccct
      361 gcggcgtttc gacggcgcgg cggcccaacc gttgacgctc ctgggcgtgg ccctggaccg
      421 catcgtgccc gcggaacgcg cttccctggc ggagtggctg gtgtccctgg ttccgctcga
      481 gggtcttgtc agcgcggacg tggaggccgc cctgcggcgg ttggtcttcc tgcaaatcca
      541 ggcctgattt ccgacggaaa aaatcgcccc gttttcttga cagggggtcg ggcccttggt
      601 aaactttggc ttccccatga aacgacttct cgaagagacc tccgacatcg ttcgttaccg
      661 cggcgggttc ggccattgga ccttcctcct ccaccgcctg acgggcttgg gggtcctggt
      721 cttccttctg gcccacgtcg tcgacacggc gctcctgggc tggggcccgg acgtcttcaa
      781 ccgtgtcatg gccgtctacc gacacccctt cttccgcctc agcgaagtgt ttctcgtggc
      841 ctccgtcctc ttccatacct taaacggggt tcggctcatc gtggtcgatt tttggccccg
      901 agccacccgg caccaccggg ctctggttcg tggcacctgg gttctgttcg tcctgctcat
      961 gattccggtg gtgatcatca tggcccacca ttatctggaa gcgaatcccc gatgacgacc
     1021 ctctcctcca acgccgggcg ccccctgccg gccggcggct ttgagcggtt cgcttggttt
     1081 tttatgcggg cctccgggct cgtcctgctg gtcctcgccc tgggccacct ggccatcatg
     1141 catttgatca acagcatcga caccgtggac tacgccttcg tcgcggcccg ttacgccacg
     1201 cccttttggc ggacctacga cgggttgatg ctggtcctgg cccttttgca cggctacaac
     1261 ggcctccgcg tcgtgttgaa cgattacctc aaaagcggct ggcgcacggc cgccacctgg
     1321 ttcatgggcc tgctgaccct tgttctgttg atcctcggcc tttacgtggt ttttgcgttc
     1381 caacccgatc ttcccgataa cacggtgctt cccaatgaca cggacctcac atcatcgctt
     1441 tgattgcctg atcgtcggcg ccggcggcgc ggggctctac gcggccctgg ccctggctca
     1501 acggaagggt tcttccgtgg cggtgttgag caaactctac cccacgcgct cccacaccgg
     1561 cgcggcccag gggggcgtga gcgcggccct gggcaacacc gaggacgact cgcccgattg
     1621 gcacatgttc gacaccgtca aaggcgggga ctacctcgtg gaccaggacg ccgccgagat
     1681 tctctgccgg gacgccgtga gtaccgtcat cgaactcgag catctgggcc tgccttttaa
     1741 ccgcaccgag gacggccgca tcgaccagcg ccgattcggg ggccacaccc gggagttcgg
     1801 caaaggcccc gtccgccggg cctgttacgc cgccgaccgc acgggccaca tgattctcca
     1861 gaccctttac cagcagtgca tcaaaaacaa cgtcactttt ttcgacgaat accacgtcgt
     1921 ggatttgatc ttgagcgcgg gccgttgcgc cggcgtcgtc gcctatcaaa tttccaccgg
     1981 cgagcttcac gtgttccacg ccaagtcggt cctcttcgcc accggcggct ccggccgcat
     2041 gttcaagatc acctccaacg cccacgcgct gacgggggac ggcatggcca tcgcgtaccg
     2101 gcgcggcctg ccactggagg atatggaatt tttccagttc caccccaccg gcatctacaa
     2161 aatgggcatt ctgctttccg aagcctgccg cggggagggg gggaagctct tgaacgggaa
     2221 aggcgaacgc tttatggagc gctacgcccc caccatgctc gacctggcgc cccgggacat
     2281 gatctcgcgc ttcatccatc aggaaatccg ggagggtcgg ggcgtggacg gcaaagacta
     2341 cgtccatttg gatctgcggc atttgggggc ccaggtcatc gaggaaaaac tccccgacat
     2401 caccgatttt gtccgcacgt atatgggctt ggaccccgtg accgatttga ttcccatcca
     2461 agccaccgcc cattacgcca tggggggaat ccccacgacc gtggacggcg aagtcatcgt
     2521 tgacgccaaa aacaccgtcg tgcccggctt ttacgccgcg ggcgaagtgg cctgcgtctc
     2581 cgtccacggc gccaaccgac tggggaccaa ctccctggtg gatattttgg ttttcggccg
     2641 ccgctccgga ctggccatgg cggagtacgc cgccaaaaac gatttcacgc ccctcccggc
     2701 ggaccccgcc gccgacgttc gcgagcgggt ggcccgcctt ctggccaatc cgcccaagca
     2761 gtcggcggcc gcccttcggg ccgaactcca ggccgccatg atggacgact gcggcgtcta
     2821 ccgatcggaa gcgggcctta aaaaagttca ggccaaaatc gtcgaatgcc gcgcgcgcta
     2881 cgcgggcgtc ggcgtggcgg acaaagggaa acagtttaac acggaactcc tggaagccct
     2941 ggagttggaa aatttgatcg acctggcgga gtgcaccgtg gccagcgcca tcgcccggaa
     3001 ggagtcccgg ggcgcccacg cccgggagga tttccccaaa cgcgacgacg cccaatttct
     3061 gaagcacacc ctggcgacca agacgccgac gggggtgtcc ctggattata aacccgtggt
     3121 gatcaccaaa ttccaacccc aggaacggaa atactaatga aggtcacgct caaaattcgc
     3181 cgcgtcgatc ccgaaaaggg ccttcccgaa cgctgggaga cttacacggt cgacgtggcc
     3241 ccgacggatc gggtcttgga cgccttgaac gaggtcaaat ggacccagga aggtcccttg
     3301 gcctaccggc ggtcctgcgc ccacggcatc tgcgggtccg acgccatgcg catcaacggc
     3361 cgcaaccgcc tggcctgcaa ggtgttggtc caggacgtgg ccccggtcat caccctggag
     3421 cccatgaagg gcttccgggt catcaaggat ttgatcgtcg acatggaacc tttcctggcc
     3481 aagtttcggt ccctgaagcc ctttttaatc aacgacgaac ccgtgaccga gggggaacgc
     3541 ctccaatcgc cggcggcccg ggcccggttc gacgacacca ccaaatgcat cctgtgcggc
     3601 gcctgcacca ccagttgccc ctccttctgg gcggacccgg cctacgtggg tcccgccgcc
     3661 atcgtgaacg cccaccgttt tattttcgac gaccgcgacc gggccgccgc cgagcgcctg
     3721 gaactgttgg acggccggga cggcgtgtgg aaatgccgaa ccgttttcaa ctgcgtggaa
     3781 gcctgccccc gcgacatcga catcacgggc gccatcgccg acgtcaagc
//
LOCUS       DAOQLS010000124         3811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12266_length_3811_cov_3.540202, whole genome shotgun sequence.
ACCESSION   DAOQLS010000124 DAOQLS010000000
VERSION     DAOQLS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12266_length_3811_cov_3.540202"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PK362_03290"
     CDS             complement(<1..510)
                     /locus_tag="PK362_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73898.1"
                     /translation="MREKINQLLASPGGQEALRLAAWVVGLSVGGVLLLWTLSSRGRW
                     TVPIPTAAQAPRLFPSSAPERVTEGRFTHGTMLYTVLSAASLPPQKAQSVAAALTGVL
                     DPRTLNEADRYELIHSTAGDFGRLSIVRKLERFVVESAPTGLVARKEPLPLATREKSV
                     AGTLNDSLWA"
     gene            666..2756
                     /locus_tag="PK362_03295"
     CDS             666..2756
                     /locus_tag="PK362_03295"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNC73899.1"
                     /translation="MMTFVFGVCALSLVVAAYLTRYVLARDTGTPEMRRIADAIRTGA
                     EAFLHRQNKTITLLFLIVAGGIFSLYALIRRDVSEGLDAAFSFLFGSCTSLLAGLIGM
                     QVSIRTNIRVASAARRSLSEALVLALRGGAVSGLAVVTLSLMGVGGLFWFMGGLADPR
                     TVPLKIVGFGFGASFVALFAQLGGGIFTKAADVGADLVGKIEVGIPEDDPRNPAVIAD
                     LVGDNVGDCAGRGADLFESTAAENIGAMILGASLYPVFGVYGILFPLVARATGLLAAI
                     GGVFAVRLKNETEDPMKALNRGYLVTSLLAVGGFYLTVRLLLKNNPWLFVAGLIGIVA
                     SYLFVWITQYYTESRYRPVKEIARSSLTGHATLIISGLGIGLESTAFPILTIAAALVG
                     AYHCGVHALAGTAATPTAAGLYGTAIATMGMLSTCGYVLAMDTFGPITDNAGGIVEMS
                     HQPDSIRRRTDRLDAVGNTTKALTKGYAVGSAALAAFLLFSAYLDEISHLIGKPFQTV
                     DIAKVEVFVGALLGAMLVLLFSALAIRAVGKAATYVINEVRSQFSERPGILQGTQDPD
                     YARCVDIVTSGALKQMVWPGVLVVGMPVAVGLIFKQFGLGAETVAGFLMVGTITGILM
                     ALFMNNSGGAWDNAKKFVETNEIYGGKGSLAHQAAVVGDTVGDPLKDTAGPSLHVLIK
                     LLSTITLVFAPLFV"
     gene            complement(2772..3383)
                     /locus_tag="PK362_03300"
     CDS             complement(2772..3383)
                     /locus_tag="PK362_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73900.1"
                     /translation="MKNANRWNSARARGPLGALGAGLTFLFWTNALAAETLPAAPPTP
                     ASDDSAVASADPNSEPRPQLLPGAFSPLEKSADAMVTALFRLMPGRRHLPSHLQKREH
                     DDYPAVFRWMPRSWTSFGWGRPRLMVGNQNTWYEGAPKPVGEKGTFQISRYPDLPPPL
                     CWLPLYFAVTTPNGVHLRLGARWDDVDAYVQFPSIAWKLDVKE"
     gene            3590..>3811
                     /locus_tag="PK362_03305"
     CDS             3590..>3811
                     /locus_tag="PK362_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73901.1"
                     /translation="MIEWLSIVVSPILGAGAGTIFKKWMNRKPPPLPRETPAGSLGAL
                     ARQVEDLQEWRGDALTVLQAHERRLQKLRN"
BASE COUNT          621 a         1177 c         1282 g          731 t
ORIGIN      
        1 ggcccacagg gaatcgttga gcgtgcccgc cacggatttt tcccgggtcg ccagcgggag
       61 gggttccttt cgcgccacga ggcccgtcgg ggccgattcg acgacgaacc gttccaattt
      121 gcgtacgatg gaaagccggc cgaaatcccc cgccgtggag tggatcaatt cataccggtc
      181 ggcttcgttg agcgttcggg ggtccagaac gccggtcaag gcggcggcga cggactgggc
      241 tttttgcggg gggagcgagg cggcgcttaa caccgtgtac agcatggttc cgtgggtgaa
      301 gcggccttcc gtgacgcgct cgggggccga agaggggaag agtcgggggg cttgcgcggc
      361 cgttggaatc gggaccgtcc agcggccccg ggaagacaac gtccaaagga gaagaacgcc
      421 gccgacggaa agacccacca cccaggccgc caggcgcagg gcttcctgcc ctcccggcga
      481 agccaacaac tgatttattt tttcccgcac ggcgacatta tagggccctc gtcggtgact
      541 gtcaacggcg cggggggggg gcggtcccct tgacgcgggc gcggcgaccc gcgcgtccgc
      601 cgaaaagatc ccgcgcggtg aggcttgcga acgccgaggc gcgaaatgct aaaatgcgcc
      661 acaccatgat gaccttcgtt ttcggcgtgt gcgctttgtc cctggtggtg gccgcctacc
      721 tgacgcggta tgtgttggcc cgggacaccg gcaccccgga gatgcgacgc atcgccgacg
      781 ccatccgcac cggcgccgaa gccttcctcc atcggcaaaa caagaccatc acgctccttt
      841 ttctcatcgt cgccgggggc atattttccc tctacgccct cattcgtcgg gacgtgtccg
      901 aaggcttgga cgccgccttc tcttttctct ttggttcctg cacgtcgctt ttggccggcc
      961 tcatcggtat gcaggtgtcc atccgaacga acatccgggt ggcctccgcc gcccggcgga
     1021 gcctttccga agccctggtg ctggcccttc gggggggcgc ggtcagcggg ctcgccgtgg
     1081 tgaccctgag cctgatgggc gtcggcggcc tcttttggtt tatgggggga ctggcggacc
     1141 cccggacggt gcctctcaaa atcgtcgggt tcggtttcgg cgccagtttc gtggcgctct
     1201 ttgctcaact gggcggaggg atcttcacca aggcggccga cgtcggggcg gatttggtcg
     1261 gtaaaattga agtcggcatt cccgaggacg atccccggaa cccggcggtg atcgccgatt
     1321 tggtcggtga caacgtgggc gactgcgcgg gacgcggcgc cgacctcttt gaatcgaccg
     1381 ccgcggaaaa catcggcgcc atgattttgg gcgcctccct gtatccggtg ttcggcgttt
     1441 acggcatcct gtttcccctg gtggcccggg ccacgggact cttggcggcc atcggcgggg
     1501 ttttcgccgt gcggctcaag aacgaaaccg aagacccgat gaaggccctc aaccgcggtt
     1561 acctcgtgac gagccttctg gcggtcgggg ggttctatct gacggtgcgc ctattgctta
     1621 agaacaaccc ctggcttttc gtggcgggac tgatcgggat cgtcgcttcc tatctcttcg
     1681 tttggatcac ccagtattac acggaatccc gttatcggcc ggtcaaggaa atcgcccggt
     1741 cctccctcac gggccacgcg acgttgatca tttcgggtct gggcatcggg ttggaaagca
     1801 cggcgtttcc gattttgacc atcgcggcgg ctttggtggg cgcctatcat tgcggggtcc
     1861 acgccctggc cggcaccgcg gccaccccga cggcggcggg tctttacggg acggccatcg
     1921 ccacgatggg gatgctgtca acctgcggtt acgtgctggc catggacacg tttggtccga
     1981 tcacggacaa cgccgggggg atcgtcgaaa tgagccacca gcccgattcc atccggcgcc
     2041 gcacggatcg gctggacgcc gtcggcaaca ccaccaaggc cctcacgaag gggtacgcgg
     2101 tgggttccgc cgcgttggcc gccttccttt tgttctccgc ctatttggat gagatttccc
     2161 acctcatcgg caagccgttc cagacggtgg acatcgccaa agtggaggtg ttcgtggggg
     2221 cgcttctggg cgcgatgctc gtgctgttgt tcagcgccct ggccatccgc gccgtgggca
     2281 aggcggccac ctacgtgatc aacgaagtgc gctcccagtt ttcggaacgc ccgggcatcc
     2341 ttcagggaac ccaggacccg gactacgcgc gttgcgtcga catcgtgacc tccggcgccc
     2401 ttaagcaaat ggtttggccg ggggtgctcg tggtcggcat gccggtggcc gtgggtttga
     2461 ttttcaagca attcggcctg ggggcggaga cggtggccgg gttcctcatg gtgggaacca
     2521 ttacggggat tctcatggcg ctctttatga acaacagcgg cggggcctgg gacaacgcca
     2581 aaaaatttgt cgagacgaat gaaatttacg gcggcaaggg ctccctggcc catcaagccg
     2641 ccgtcgtggg ggacaccgtg ggcgatccct tgaaggacac cgcggggccc agccttcacg
     2701 ttctgatcaa gcttctctcc accatcaccc tcgtcttcgc tcccctcttc gtttaaaccg
     2761 actctccccg tttattcctt cacgtccagt ttccaggcga tggaggggaa ctgcacatag
     2821 gcgtccacgt cgtcccagcg ggcgcccaac cgcaggtgaa ccccgttggg cgttgtcacc
     2881 gcgaaataaa gcggaagcca gcagaggggc ggcgggaggt ccgggtagcg cgaaatttgg
     2941 aaggtcccct tttcgccgac gggtttcggc gccccctcgt accaggtgtt ttgattcccg
     3001 accatgagcc ggggccggcc ccagccgaag gacgtccagg agcggggcat ccaacgaaag
     3061 accgccgggt agtcgtcgtg ttcacgcttt tgcaggtgcg aaggcaggtg gcgccggccg
     3121 ggcatgagcc gaaagagagc cgtcaccatc gcgtcggcgg atttttcgag gggcgaaaag
     3181 gccccgggca ataattgcgg gcggggttcc gaattcggat cggcggaagc cacggcggaa
     3241 tcgtcgctcg cgggcgtggg aggggcggcg ggaagggttt cggcggcgag cgcgttcgtc
     3301 cagaagagaa acgttagtcc cgcgcccagg gcgccgagcg gcccacgggc tcgagcagaa
     3361 ttccatcggt tcgcgttttt catggcacga tcccccgatc ggggtggtga tcccccatcg
     3421 ggaagccgcg cgcggtgtcg agtgtttctc cgccttaagc ctaccccccc gtcgtcggaa
     3481 attcaagggg acgcgggggg gggcggccgc ccacggcccc atcgctccgc gttgggctgg
     3541 caaaccctta aaacgtggcg catactggag gggaatttgg gaggtccctt tgatcgaatg
     3601 gctgtccatc gtggtgtcgc ccatcctggg cgccggcgcg ggcacgattt ttaagaaatg
     3661 gatgaaccga aagcctccgc cgttgccccg ggaaaccccg gccgggtccc tgggcgccct
     3721 ggcgcggcag gtggaggatt tgcaggaatg gcggggggac gccctgacgg ttctgcaggc
     3781 ccacgagcgg cggctgcaga aattgcgcaa c
//
LOCUS       DAOQLS010000125         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12277_length_3809_cov_3.892648, whole genome shotgun sequence.
ACCESSION   DAOQLS010000125 DAOQLS010000000
VERSION     DAOQLS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12277_length_3809_cov_3.892648"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK362_03310"
     CDS             complement(<1..250)
                     /locus_tag="PK362_03310"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNC73902.1"
                     /translation="MTVKALVLRSPGTNCDGESVWALEAAGAAPERIHVNALLRGEAR
                     LKDFGVLVFPGGFSFGDDIASGKVLANRVVYRLREQMDD"
     gene            complement(247..2667)
                     /gene="purL"
                     /locus_tag="PK362_03315"
     CDS             complement(247..2667)
                     /gene="purL"
                     /locus_tag="PK362_03315"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNC73903.1"
                     /translation="MVAAPPAPLPGFLSLDAAALAALSKERGLSLNDAEMAAIQDYFK
                     KQGRDPSEAELETLAQTWSEHCKHKTFRAAIHHVERDEMGLKREREYKDLLKDTIVKV
                     TDELKRPWCLSVFKDNAGIVDVGENEALAFKVETHNHPSALEPYGGAGTGLGGVIRDI
                     LGAGLGAKPIANSDVFCFGPLDKTADEKTGSGMSPRRIFHGVVSGVRDYGNRMGIPTV
                     SGAVYFDEDFRENPLVFCGTLGIMPHQAIPKDVKPGDKIVMAGGRVGRDGIHGATFSS
                     DSLTTGIPSSVVQIGNPIVQKKTMDVLLAARDRGLYRGITDCGAGGLSSAVGEMGEIT
                     GADVQLEKVPLKYQGLAPWEIWLSESQERMVFAVPPEKVDELVRLFADEDAEATVIGT
                     FTNDGRLRVRHGADVLVDVEMAFLHNGVPKRRMTSTWAPPTPEAFPAFHVGRKTVDLR
                     KALLDILAQPTVASKEWIVRQYDHEVQGRTVVKPFVGPTAAGPSDAAVLQLRPTDRRG
                     AALSCGLNPHQSRWDPYWMAASAIDEAVRNLIAVGARPDRIAILDNFCGGNPYRPETL
                     GELVRTAQACHDVARAYGTPFISGKDSLFNEFVDPKAGSRRSIPTTLLISALSLVDDV
                     SKCVTMDFKKPGNFIYVLGETRDELGGSRYGIWRNTRGGAVPRLDPAATWPIYEKLAQ
                     AIGEGLVAACHDCSEGGLGVALAEMAFGGGLGAHVDLRKALLSADLANERRTDKILFS
                     ESNGRFVIEVPAKARRGFNALFKNFPVAAVGQVREAPRLDVTGADGKPLSWSVKDLEK
                     AWRGGIRP"
     gene            complement(2667..3128)
                     /locus_tag="PK362_03320"
     CDS             complement(2667..3128)
                     /locus_tag="PK362_03320"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNC73904.1"
                     /translation="MNWRVLVHTKPAFADHRGRGLAHEWRRAKLKGLKTIRTGQAYEL
                     LGELDQNQAQGLADRLLTDPVTQVASVFPSDRVAPAPGSRLAEVWPKKGVADPVAGTV
                     VYAAQDLGVAGLLAVRSGQVYDFFGAVSPADVKRFCEEHLMNAMIQSVEVL"
     gene            complement(3125..>3809)
                     /locus_tag="PK362_03325"
     CDS             complement(3125..>3809)
                     /locus_tag="PK362_03325"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNC73905.1"
                     /translation="ICPNHLIATRVADFPADLRAVCAEVDGRAVLVKKAKRVDVECVV
                     RGYLAGSGWKEYQASQTVCGEKLPAGLVESSKLPTPIFTPATKAPDGEHDENISFKRM
                     EDLVGSGLAGRLKALSLRLFNEATHYAESRGFILADTKFEFGLIGGEIILIDEALTPD
                     SSRFWDAAHYAPGKSQESFDKQFVRDYLNQIKWDRRPPAPALPAEVVAKTHEKYMQAY
                     QRLTGRAAL"
BASE COUNT          649 a         1204 c         1272 g          684 t
ORIGIN      
        1 aatcgtccat ctgctcccgc agacggtaca ccacgcggtt ggccagcact ttcccggaag
       61 cgatgtcgtc cccgaaggaa aacccgccgg ggaacaccag aacgccgaag tctttcaggc
      121 gcgcctcgcc gcgcaaaagc gcgttcacgt gaatccgttc gggcgcggcg ccggcggcct
      181 cgagggccca cacggactcg ccgtcgcaat tggtgccggg ggaacgaaga accagggctt
      241 tgacggtcac gggcgaatcc ccccccgcca ggctttttcc aggtccttca cggaccaaga
      301 caggggtttg ccgtcggcgc cggtcacgtc caaccggggg gcttcccgca cctggcccac
      361 ggccgcgacc gggaagtttt tgaaaagggc gttgaacccc cggcgggcct tggcgggcac
      421 ctcgatcacg aagcgcccgt tggattcgga aaacaggatt ttgtcggtcc gacgctcgtt
      481 ggcgagatcg gcggaaagca gcgcttttcg aaggtccacg tgggccccca ggccgccgcc
      541 gaaggccatc tcggccaggg ccacgcccag gccgccttcg gagcaatcgt ggcaggccgc
      601 cacgaggccc tcgccgatgg cctgggccaa tttttcgtag atcggccagg tggcggccgg
      661 gtccaggcgc ggcacggcgc cgccccgggt gttgcgccaa attccgtagc gggacccgcc
      721 gagctcgtcc cgggtttcgc ccagaacgta aatgaagttt ccgggtttct tgaaatccat
      781 ggtgacgcat ttggacacgt cgtccacgag ggaaagggcc gaaatcaaaa gcgtggtggg
      841 gatgctccgg cgacttccgg ccttggggtc cacgaactcg ttgaagaggc tgtccttgcc
      901 cgaaatgaac ggggtgccgt aggcccgggc cacgtcgtgg caggcctggg cggtgcgaac
      961 caattcgccc agggtttcgg ggcggtaggg attgcccccg caaaaattgt ccaggatggc
     1021 gatgcggtcc ggtcgggcgc ccacggcgat caaattccgc accgcctcgt cgatggccga
     1081 ggccgccatc cagtaggggt cccaccggct ttggtggggg ttcaacccgc acgagagggc
     1141 cgcgccgcgg cggtccgtgg gccgcaactg caaaaccgcg gcgtcgctgg ggcccgcggc
     1201 ggtgggcccc acgaagggtt tcaccacggt gcggccctgg acctcgtggt cgtattgccg
     1261 aacgatccat tccttgctgg ccacggtggg ctgggccaga atgtccagga gagcttttct
     1321 caaatcgacg gttttccgcc cgacgtggaa cgccgggaag gcctcgggcg tgggcggcgc
     1381 ccaggtggag gtcatgcgcc gtttgggaac accgttgtgc aggaacgcca tctccacgtc
     1441 gaccagcacg tcggccccgt gacgcacccg caggcggccg tcgttggtga aggtgccgat
     1501 cacggtggcc tcggcgtcct cgtcggcgaa gagacggacc agttcgtcca ctttttccgg
     1561 cggcacggcg aaaaccatgc gctcttggga ttccgacaac catatttccc agggggccag
     1621 gccctggtat ttgaggggca ctttttccaa ttgaacgtcg gccccggtga tttcccccat
     1681 ttcgcccacg gcggaagaga gccccccggc gccgcagtcg gtgatgcccc ggtaaagacc
     1741 ccggtcccgg gcggccagaa gcacgtccat ggtttttttc tgcacgatgg ggttgccgat
     1801 ttgaacgacg ctggagggaa tcccggtggt caggctgtcg gaggagaagg tcgcgccgtg
     1861 aatgccgtcc cggcccacgc ggcccccggc catcacgatt ttgtcccccg gtttcacgtc
     1921 cttggggatc gcctggtggg gcatgatccc gagcgtcccg cagaagacca aggggttctc
     1981 ccgaaaatcc tcgtcgaaat agaccgcgcc gctcaccgtg ggaatgccca tgcggttgcc
     2041 gtagtcccga acgccggaaa ccacgccgtg aaagatccgt cggggagaca tgccggaacc
     2101 ggttttctcg tcggcggttt tgtccagggg gccgaagcag aagacgtcgg aattggcgat
     2161 gggcttggcc cccaggcccg cgcccaaaat gtcccggatc acgccgccca agccggtgcc
     2221 ggcgccgccg tagggttcca gggccgaggg gtggttgtgg gtctcgactt taaaggccag
     2281 ggcttcgttc tcgcccacgt ccacgatgcc cgcgttgtcc ttgaaaaccg aaaggcacca
     2341 ggggcgcttg agctcatcgg tcaccttgac gatggtgtcc ttgagcaaat ccttgtactc
     2401 ccgttcgcgc ttcaaaccca tctcgtcccg ctcgacgtga tggatggcgg cccggaaggt
     2461 tttgtgtttg cagtgctcgg accaggtttg ggccagggtt tcgagttccg cctcggaggg
     2521 atcgcggccc tgttttttga agtagtcctg gatggcggcc atctccgcgt cgttcaacga
     2581 caatcctcgc tccttgctga gcgccgccag ggcggcggcg tccagggaga gaaaaccggg
     2641 caggggagcc ggaggggccg cgaccattag aggacctcca ccgattggat catggcgttc
     2701 atcaaatgct cctcgcaaaa acgtttcacg tcggccgggg acaccgcacc gaagaaatcg
     2761 tacacctggc ccgaacggac ggccaaaagg ccggccacgc ccaggtcctg ggcggcgtac
     2821 accacggtgc cggccacggg atccgccacc ccttttttgg gccacacttc ggccaggcgg
     2881 gaccccggcg cgggagccac ccggtccgac gggaacaccg aggccacctg ggtcacggga
     2941 tcggtcagca ggcggtcggc cagcccctgg gcctggtttt gatccagttc gcccaggagt
     3001 tcataggctt ggccggtgcg aatggttttt aggcctttga gtttcgcccg acgccattcg
     3061 tgagcgagcc cccgaccccg atgatcggca aaggcgggtt tcgtgtggac caacacgcgc
     3121 cagttcatag ggccgcccgg ccggtcaatc gttgataggc ctgcatgtat ttttcgtggg
     3181 ttttggcgac cacctcggcg gggagcgccg gcgcgggggg gcggcggtcc catttgattt
     3241 ggttcaggta gtcgcgaacg aactgcttgt cgaaggattc ctgggatttt ccgggggcgt
     3301 agtgggcggc gtcccagaac cgggaggaat cgggcgtgag ggcctcgtcg atcaaaatga
     3361 tttccccgcc aatcaggccg aactcgaatt tcgtgtcggc caggataaag ccccgggatt
     3421 cggcgtagtg agtggcctcg ttgaagaggc ggaggctcag ggccttgagc cgcccggcca
     3481 gaccggaccc caccaaatcc tccatgcgct taaaagaaat gttctcgtcg tgctccccgt
     3541 cgggggcctt ggtggcggga gtgaaaatgg gcgtgggcaa tttggaggat tccacgaggc
     3601 ccgcgggcaa tttctccccg cagacggttt ggctcgcctg gtactctttc cagccggacc
     3661 cggccaggta gccccgcacc acgcattcca cgtccacgcg tttggctttt ttgaccagca
     3721 cggcccgccc gtccacctcg gcgcagacgg cccgaaggtc ggcggggaaa tcggccaccc
     3781 gggtggcgat caaatggttg gggcaaatg
//
LOCUS       DAOQLS010000126         3806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12289_length_3806_cov_4.501733, whole genome shotgun sequence.
ACCESSION   DAOQLS010000126 DAOQLS010000000
VERSION     DAOQLS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12289_length_3806_cov_4.501733"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PK362_03330"
     CDS             complement(<1..1135)
                     /locus_tag="PK362_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC73906.1"
                     /translation="MISADGSGFSRTDDRRVAVLSGLATAVFALLVLRLFHLQIVRGP
                     DMVRQAEQNRTQIVPLLAPRGLIVDRNGDVLVDNAPRFSLFYDYNANAPVAMDQLAED
                     LTRWLPEQRALIHRKMAEARRSGKMTRLLADVPRGAALALMERRMLLPGVNVVVEPQR
                     RPRYGELASHLLGYVDEVDAGELERLRDRGFRLGQLIGKSGVEKFYDDLLRGEDGGLQ
                     FETDAAGRHVQIIRRIPSDPGDDVRLTLDRRLQAVAEEALSRSTTGRGAAVALDPRNG
                     AVRVLASRPGFDFSKGLGAYLGDPSLPLFNRALQGTYPPGSVFKIVTAAAALTQLQWD
                     TKTSYVCTGVYRLGKQEFACWSVHKRQDFFGAVAWSCNFYFYNL"
     gene            complement(1128..1646)
                     /gene="mreD"
                     /locus_tag="PK362_03335"
     CDS             complement(1128..1646)
                     /gene="mreD"
                     /locus_tag="PK362_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNC73907.1"
                     /translation="MIRRALFVLLTFFLGWIAQAIFLHLAPLSLPAPHWLLVAVLAVG
                     ASGRVNLAQGLGFFWGLALDLYGQTPFGSQGLLLAFCGFAAGSLSKNLNAEKMITQEA
                     LTLVASVVFIVGSLQLGEWFVPATGPHRWSLGLCLAGAAMNALAAPAVFWALRIWLEL
                     GDAWRARAGARD"
     gene            complement(1643..2476)
                     /gene="mreC"
                     /locus_tag="PK362_03340"
     CDS             complement(1643..2476)
                     /gene="mreC"
                     /locus_tag="PK362_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNC73908.1"
                     /translation="MFQRHRPAVVFSVYAVLGLALLSWSSDRIVQSLKYAFFYLLAPT
                     SAPAVGPLSQWGEFGHNLSLLIRADQRARAAEARWLAERLDEKRSQTIEEENRRLTES
                     AGLARWPRFSATAARVWARNANDWFHSVMVRPAAGAPVRVGDAAVTVRDGRVVILGQV
                     AEILPRGFARVLLVTDPSSSLSAHAARTDEQGLVEGRGANLLLFNYLFSDSPIQPGDE
                     VITSGLGDVFPEGVLIGRVLAVQEATHESFKRAVLAPAAKLNDVREMLLLSRRPDGES
                     P"
     gene            complement(2603..3634)
                     /locus_tag="PK362_03345"
     CDS             complement(2603..3634)
                     /locus_tag="PK362_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNC73909.1"
                     /translation="MFDYIFSLFSNDMGIDLGTANTLVYVKGQGIVLKEPSVVAINRD
                     TREPVAFGLEAKRMLGKTPANIYAVRPLRNGVIADFDVTQEMIKYFIRKVHNRRSLLH
                     PRVVIGIPSGITEVERRAVKEAADQAGARYVELIEEPKAAAIGAEMPISEPSGNMIVD
                     IGGGTTEVAVISLGGMVVSKSLDVAGDEMDESIVQYFRRKYNLLIGETTAEDVKIKIG
                     SAFPQAEEQTLEVKGRDQVTGLPKTITISSEEVRQALSEPLKLIVDVVKSTLEETPAE
                     LSADLVDRGIVLAGGGSLLRGMPELLSQETELPVTRAADPLTCVVMGCGRYLEELDNI
                     KRSRGRASV"
     gene            complement(3658..3789)
                     /locus_tag="PK362_03350"
     CDS             complement(3658..3789)
                     /locus_tag="PK362_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HNC73910.1"
                     /translation="MRKKIYDVLVPRYQSRSGGCTRVYRLGNRSGDNAEMAILKLVQ"
BASE COUNT          694 a         1256 c         1255 g          601 t
ORIGIN      
        1 cgagattgta aaaataaaaa ttgcaggacc aggccaccgc gccgaagaag tcctgacgct
       61 tgtgcaccga ccaacaggcg aactcctgtt ttcccaggcg gtaaacgccg gtgcaaacgt
      121 agctggtctt cgtgtcccac tgaagctggg tcagggccgc cgccgccgtg acgattttga
      181 acaccgaccc cggcggatag gtcccctgca aggcccggtt gaacagcggc agggacggat
      241 cgcccaaata ggccccgagg cccttggaaa aatcgaaacc cggccggctg gccaggaccc
      301 ggaccgcgcc gttccgcggg tccaacgcga cggccgcgcc ccgccccgtg gtcgagcggg
      361 agagggcttc ctcggccacg gcctggagcc ggcggtcgag ggtgagccga acgtcgtcgc
      421 ccgggtccga cgggatgcgc cggatgatct gcacgtggcg gccggccgcg tccgtttcaa
      481 actgaaggcc gccgtcctcc ccgcgcagaa gatcgtcgta aaatttttcc acgccggact
      541 tgccgatcag ctggcccagg cgaaagcccc ggtcccgcag gcgttcgagt tccccggcgt
      601 ccacctcgtc gacgtagccc aacaggtggc tggccagttc gccgtagcgc gggcggcgct
      661 ggggttccac caccacgttg acgcccggga gcaacatccg ccgttccatg agggccaggg
      721 ccgcgccccg gggcacgtcg gccaagagcc gcgtcatctt gccgctccgt cgggcttcgg
      781 ccattttccg gtggatcagg gcccgttgct ccggcaacca gcgggtcagg tcctcggcca
      841 gttggtccat ggccacgggg gcgttggcgt tgtagtcgta aaacagcgaa aagcgcgggg
      901 cgttgtccac cagcacgtcg ccgttccggt cgacgatgag cccccggggg gccaggaggg
      961 ggacgatctg ggtccggttc tgttcggcct ggcgcaccat gtcggggccc cggacgattt
     1021 ggaggtggaa cagccgcagg accaggaggg cgaagaccgc ggtggcgagt cccgacagga
     1081 ccgcgacccg ccggtcgtcg gtccgggaaa aaccggaccc gtcggcgcta atcacgggcc
     1141 cccgcccggg cgcgccaggc gtcgccgagt tccagccaaa tccgcagggc ccagaacacc
     1201 gccggggcgg ccagggcgtt catggcggcg ccggccagac acagcccgag gctccagcga
     1261 tggggccccg tggccggcac aaaccattcc cccagttgca gggagccgac gataaagacc
     1321 acgctcgcga ccaacgtcag ggcttcctgg gtgatcattt tttcggcgtt caaattttta
     1381 gagagggaac ccgcggcgaa accgcaaaag gccaggagca ggccctggga cccgaagggc
     1441 gtctggccgt agaggtccaa cgccaatccc cagaagaacc ccaacccctg ggccaggttg
     1501 acccggcccg aagcgcccac ggccaggacc gccaccaaaa gccaatgggg cgccggcagg
     1561 gacaacggcg ccaggtgcag gaagatcgcc tgggcgatcc accccaggaa aaacgtgagc
     1621 aacacgaaga gggcccggcg gatcacgggg actccccgtc gggacggcgg gagaggagca
     1681 gcatctcccg aacgtcgttc agtttggccg cgggcgcgag gaccgcgcgt ttgaaacttt
     1741 cgtgggtggc ttcctggacc gccagcaccc gaccgatcaa gacgccctcg gggaaaacgt
     1801 cgcccaggcc ggaggtgatc acttcgtccc cgggctggat gggcgaatcg gaaaacaaat
     1861 agttgaagag gaggaggttg gcgccccggc cctcgaccaa accctgttcg tcggtgcgcg
     1921 ccgcgtgggc cgacagggag gacgacggat ccgtcaccag gagcacccgg gcaaaccccc
     1981 ggggcaggat ctccgccacc tggcccaaaa tgaccacgcg gccgtcccgc acggtcacgg
     2041 cggcgtcccc cacgcgaacc ggggctccgg ccgcggggcg gaccatcacg ctgtggaacc
     2101 aatcgttggc gttccgggcc cacacccggg cggcggtggc ggaaaatcgc ggccaccggg
     2161 ccagcccggc ggattccgtc agccgccggt tctcttcctc gatggtttgg gaacgtttct
     2221 cgtccaggcg ttccgccagc cagcgggctt cggcggcccg ggcccgttgg tcggcgcgga
     2281 tcaagagcga caggttgtgg ccgaattccc cccactggga gagggggccg accgcggggg
     2341 cgctggtggg ggcgagcagg tagaaaaagg cgtacttgag gctctggacg atccggtccg
     2401 acgaccagga caggagcgcc aggccgagga cggcgtagac ggaaaagaca accgctggac
     2461 gatgccgctg aaacaaatcg gtctccccga ccctcccggt ggagggttcc cccgaagggg
     2521 gtcccgacgc gtttcggcgc gaaaaaagcg cccgcgatcg ggggatccga aaagggaatg
     2581 cggcagcgag ggaggggtcg gcttagacgg aagcgcggcc ccgggagcgc ttgatgttgt
     2641 caagctcctc caggtagcgg ccgcaaccca tcacgacgca cgtcagggga tcggccgccc
     2701 gggtcacggg cagttcggtc tcctgggaca acaactcggg catcccgcgc aggaggcttc
     2761 cgcccccggc cagcacgatg ccgcggtcca ccaagtcggc cgacagttcc gccggcgttt
     2821 cttccaaggt cgacttgacg acgtccacga tgagcttcaa aggttcggag agggcctggc
     2881 ggacttcctc ggacgaaatg gtgatcgtct tcggcaaccc cgtcacttgg tcccgcccct
     2941 tcacttcgag cgtttgttcc tcggcttggg ggaaggcgct accgatcttg atcttcacgt
     3001 cttccgccgt ggtttcgccg atgagcaaat tgtatttccg tctgaagtac tgaacgatgg
     3061 actcgtccat ctcgtccccg gccacgtcca gggacttgga gaccaccatt ccccccaagg
     3121 aaatcacggc cacttcggtg gtgccgccgc cgatgtccac aatcatgttg cccgaaggct
     3181 ccgagatggg catttcggcg ccgatggcgg ccgccttggg ttcctcgatc aactccacat
     3241 accgcgcgcc cgcctggtcg gccgcctctt tgacggcccg ccgctccacc tcggtgatcc
     3301 ccgaggggat cccgatcacc acgcgcggat ggagcaagct tcggcggttg tgcacttttc
     3361 ggataaaata cttgatcatt tcctgcgtca cgtcgaaatc agcgatcacc ccgttgcgca
     3421 ggggacgcac cgcgtaaatg ttcgccgggg ttttgccgag catgcgcttg gcctcgaggc
     3481 cgaaggccac gggttcccgg gtgtcgcggt tgatcgccac gaccgaaggt tccttgagga
     3541 cgatcccctg ccctttgacg taaacgaggg tattggccgt tcccaggtca atccccatgt
     3601 cgttcgaaaa gaggctgaag atgtagtcga acacgtttcc cctcggtggc ggtcccttta
     3661 ttgaaccagt ttcaggatcg ccatttcggc gttgtcgccg gaccggttgc ccaatcggta
     3721 cacgcgggtg catccgcccg aacgcgactg gtaacgcggc accaaaacat catatatctt
     3781 tttacgcacg tccttgtcgt tgatcg
//
LOCUS       DAOQLS010000127         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12431_length_3782_cov_3.766032, whole genome shotgun sequence.
ACCESSION   DAOQLS010000127 DAOQLS010000000
VERSION     DAOQLS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12431_length_3782_cov_3.766032"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="PK362_03355"
     CDS             <1..1355
                     /locus_tag="PK362_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73911.1"
                     /translation="GMAFLVFAGAAYFGFDAAAAGSAGQMVLGGFVGMLGSWGSLFYG
                     RHLALGLAAVAVRPGSGERATLNLDALAARHGLASLEATRDLPAGVFAEIEPATGRGF
                     IASWLVTPWSRADAVRASVVALVLTHEAQRRRLSRLELLQTPVLYLTKPFFRAGTFSA
                     VRDRWKEGFRAAWSGMGEWAQMGSGLFSYATNAGVKAFPAAPALMVAGPVAGLGGSTF
                     LTDLLGLVAGPTVWSLPDSLHSLLRGGGFLGAPGVAESRKGDALVVSLPEGKLSPAVG
                     RALLQFLSGIGARLESDNGRAPVHVELVIGAAAPSADQRGQLMAAFRQLAQSGGVSKD
                     LQDRLTIDVTHRAPEVLKTRLGELAQTSNINVLSSGDEAPFWFGLGVPLRVFAVRLLE
                     KLTQVEVEVVFTGAEAEAVARLSGQRVENGQLRLKALGRPLDILDADTRKIETFNQQA
                     "
     gene            1486..2880
                     /locus_tag="PK362_03360"
     CDS             1486..2880
                     /locus_tag="PK362_03360"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNC73912.1"
                     /translation="MKRLLPILALLCLWVIARAGKNPLPEPDQQSRVYRFKPVQFKTI
                     DSYSDEKPLLNQIARDTWGYFRDLVDKQTGMPLDNVLIGSTYTKVNSYTSTTNMGLYL
                     MCLVSAEDFGYITRTESLARARRLLSTLREVDHWEGQFFNYYETITTDASSRFVSSVD
                     NGWLAAGLIVARQAYPELESEIDPMLRQMDFSKLFDPEAGQLYLGYDADKKKPSDSHY
                     GLLCTEPRLASYIGIAKGDLPHDHWYRLFRTLPLDWDWQNQKPKGKIVEKEGVPVFYG
                     YYERNKVRWVPSWGGSLFEFLMPTLVLDEGRLSPKGLGANNKIALDAQIRYALEERRY
                     PVWGLSPCATPDDPLGYKEYGVPYLGAKGYRDDGVVTPHVSFLALSVDAPRAIENIRR
                     LARFRNFYGEYGFYDSINVKTGRISPRYLALDQAMTLVALNNYLNNGAIQKRFWSHSL
                     IQAQSRLLRDEVFF"
     gene            2924..3670
                     /locus_tag="PK362_03365"
     CDS             2924..3670
                     /locus_tag="PK362_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNC73913.1"
                     /translation="MTHPDPDLSAPYFVEILAGLRRSLGAIRVNPPRGTPLTGLVRVA
                     PEGKASLPAGWRRLPTVVVSGRSATAPSWDVDNVVSARRLVGLLIRRGHRRIALINGK
                     LDTANGRDRRRGFRQALRKAGIRPIPALEVEGRFDRERGRKAMKWLLNLKRPPTAVFA
                     ANDAMALGALEELRYRRCRGVAVAGFDDVPEARRAGLTTVRPPLRRLAVQAGRALRRW
                     VQRDERPAARAIVVPARIVIRTSSMKRVVI"
BASE COUNT          646 a         1160 c         1202 g          774 t
ORIGIN      
        1 cgggcatggc tttcctggtt ttcgcggggg cggcctattt cggcttcgac gccgcggcgg
       61 cgggatccgc cgggcaaatg gttttgggcg gtttcgtggg aatgctgggg tcctggggct
      121 ccctctttta cggtcgccat ctcgccctgg ggctggcggc ggtcgcggtg cggcccggtt
      181 ccggcgaacg ggcgaccttg aatttggacg cgctggccgc ccgccacggc ctggccagtc
      241 tggaagcgac gcgggacttg cccgcggggg tattcgcgga aattgaaccc gccacgggac
      301 gcgggttcat cgcttcctgg cttgtgaccc cctggtcccg ggccgacgcg gttcgcgcct
      361 cggttgtcgc gctcgttttg acccacgaag cccagcgccg tcgcctgagc cgtttggaat
      421 tactgcaaac gcccgttttg tacttgacca agcccttctt ccgcgccggg acattctcgg
      481 cggttcgcga tcgatggaag gaaggattcc gagccgcgtg gtcggggatg ggcgaatggg
      541 cgcaaatggg ttcggggttg ttcagttacg cgacgaacgc gggcgtcaaa gcgtttcccg
      601 cggcgcccgc gttgatggtg gccggccccg tggcgggcct gggcgggtct actttcctca
      661 ccgacctttt gggattggtc gcgggtccga cggtctggag cttgcccgat tcccttcatt
      721 cccttctgcg gggcgggggg ttcctgggcg cgcccggggt cgcggaaagc cggaaaggcg
      781 acgcgctcgt ggtcagtctg cccgagggga aactttcgcc ggccgtgggc cgggcgctcc
      841 ttcaattcct ttccggcatc ggcgcccgcc tggaatcgga caacgggcgg gcccccgtgc
      901 acgtggagct ggtcatcggc gccgccgccc cctccgccga tcagcggggg cagctgatgg
      961 ccgccttccg ccaattggcc caatccggcg gcgtttccaa ggacctgcag gatcgcctga
     1021 ccatcgacgt cacccaccgg gcgcccgagg ttttgaaaac ccggttgggc gaattggccc
     1081 agacctcgaa catcaacgtt ctttcctccg gcgacgaagc ccccttctgg ttcggcctcg
     1141 gggttcctct tcgggtcttc gcggtgcgcc tgctggagaa gttgacccag gtggaagtcg
     1201 aggtggtgtt caccggcgcg gaagccgaag ccgtggcccg gttgagcggc cagcgggtcg
     1261 aaaacggtca attgcgcttg aaagccctcg gtcggccctt ggacattttg gacgccgaca
     1321 cccggaaaat cgagacgttc aaccaacagg cctaggaccc cgggagcccc gaaatcccgg
     1381 gggtggacgg ggccgttttc cttgttttgt tcggggtctt ttttgctaca ctccaaggag
     1441 ccggacgccg cggtccccgt ccacactcat tgctgagaag ggtacatgaa gcgactcctc
     1501 cccatcctgg ctctcctttg tctctgggtt attgcccgcg cgggcaaaaa ccccctgccg
     1561 gaacccgatc agcaatcccg ggtttaccga ttcaagcccg ttcaattcaa aaccattgat
     1621 tcctattccg atgaaaaacc cctcttaaac caaatcgccc gggacacctg gggctacttc
     1681 cgcgatttgg tggacaaaca gaccggcatg cccctggaca acgtgctgat cggctccact
     1741 tacacaaaag tcaacagcta cacctccacc accaacatgg ggctttacct catgtgcctg
     1801 gtttccgccg aagacttcgg atacataacg cggacggaaa gcctcgcccg ggcgcggcgc
     1861 cttctgtcga cgcttcggga ggtggaccat tgggaagggc aattttttaa ctattacgaa
     1921 accatcacca cggacgcctc gtcgcgtttc gtgtcctcgg tggacaacgg ctggctggcc
     1981 gcggggctca tcgtggcccg acaagcctac cccgaattgg aatcggaaat cgatccgatg
     2041 cttcggcaaa tggatttttc gaaattgttc gatcccgagg cgggtcagct ctatttggga
     2101 tacgacgccg acaaaaagaa gccctcggac tcccattacg gcttgctgtg cacggaaccc
     2161 cgtctggcct cctacatcgg catcgccaag ggcgatttgc cccacgacca ttggtatcgg
     2221 ctttttcgaa ccctccccct ggattgggat tggcagaatc aaaaacccaa ggggaaaatc
     2281 gttgaaaaag aaggcgttcc cgttttttac ggctattacg agcgcaacaa agtccgttgg
     2341 gtgccgtcct ggggcgggag cctttttgaa tttttgatgc cgaccctcgt gttggacgaa
     2401 ggccgtctat cgcccaaggg gttgggcgcc aacaacaaga tcgccctgga cgcccagatc
     2461 cgctatgccc tcgaagaacg gcgttacccg gtgtggggcc tttccccctg cgccacgccc
     2521 gacgatcccc tgggctacaa ggaatacggg gttccctact tgggggccaa gggctaccgg
     2581 gacgacggcg tggtcacccc ccacgtgagt tttttggcct tgtccgtcga tgcgccccgg
     2641 gccatcgaaa acattcgccg tctggcgcgt ttccgaaatt tctatgggga atacggattt
     2701 tacgacagca tcaacgtcaa gacgggtcga atttccccgc gttatttggc cttggaccag
     2761 gccatgacgt tggtggccct gaacaattac ctgaacaacg gcgcgattca aaagcggttt
     2821 tggagccatt ccctgatcca ggcccaatcc cggctactcc gcgacgaggt ttttttttaa
     2881 gatataacgg tccgaatcgg tgatcccccg gaccgttggg gttttgacgc accccgaccc
     2941 tgatctttcc gcgccctatt tcgttgaaat tctcgccggc cttcgccggt cgttgggagc
     3001 cattcgcgtc aatccccccc ggggaacgcc gctcacgggc ttggttcggg tggcgccgga
     3061 aggaaaggcg tccctgccgg cgggatggcg gcgactcccg acggtggtgg tcagtggccg
     3121 atccgcgacg gccccttcct gggacgtcga caacgtggtc tcggcgcggc ggctggtggg
     3181 tcttctgatt cggcgcggcc atcggcgaat tgctttgatc aacggaaagt tggacacggc
     3241 caacgggcgg gaccgccgcc ggggttttcg gcaggccctg cgaaaagcgg ggattcgtcc
     3301 gatccccgcc ctggaagtcg aagggcgctt tgatcgagag cgcggccgta aggccatgaa
     3361 gtggttgttg aatttgaaac ggccgccaac cgcggtgttc gcggccaacg atgccatggc
     3421 cctgggggcc ctcgaagaat tacgatatcg gcgatgccgg ggcgtggcgg tggcgggttt
     3481 cgacgatgtc ccggaggctc ggagggcggg cttaaccacc gttcgaccgc ctcttcgtcg
     3541 gctggcggtc caggcgggcc gggctcttcg ccggtgggtt caaagggacg agcgcccggc
     3601 ggcaagggcg attgtggtcc cggcccggat cgtgattcga acgtcatcga tgaaacgtgt
     3661 tgttatttag aagcaaagaa cgaaagtcag gagacgatgt gaacgtcacg aaacgacgtt
     3721 ggatgctggt cgccgctctg gccgccgcca gcgtcgtgtc gggaaacgca cgcgcgcaag
     3781 gc
//
LOCUS       DAOQLS010000128         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12531_length_3765_cov_3.616981, whole genome shotgun sequence.
ACCESSION   DAOQLS010000128 DAOQLS010000000
VERSION     DAOQLS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12531_length_3765_cov_3.616981"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PK362_03370"
     CDS             complement(<1..147)
                     /locus_tag="PK362_03370"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365763.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HNC73914.1"
                     /translation="MPVDESGKKKSVPHGLWTKCPKCEQIIYNKELEKNLKVCSKCGT
                     PLRTT"
     gene            complement(157..972)
                     /gene="trpA"
                     /locus_tag="PK362_03375"
     CDS             complement(157..972)
                     /gene="trpA"
                     /locus_tag="PK362_03375"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNC73915.1"
                     /translation="MFAGLKKEKRSAFIAYLTGGFPTLSGMDRAAQDLVRAGVDILEI
                     GVPFSDPIADGPTIQFASQKALEGGATPDKILRWARGFRRKHHVPLVLMTYMNPVARR
                     GFARFARDARASGVDGLIVPDLIPEEAAPLRAALTKQGIHLIHLVAPTTPSARRRWIA
                     RQSGGFLYAVSVTGVTGARKGFSSDLPAFLKGLRRGSPVPVAVGFGVATAGDARRLAP
                     LADGVIVGSAFIQRLRNKESVAGLARALRAVLSQSSRTSGGRGAAETPDALNR"
     gene            complement(1107..1286)
                     /locus_tag="PK362_03380"
     CDS             complement(1107..1286)
                     /locus_tag="PK362_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73916.1"
                     /translation="MADFSFTDRQVTHLLMALRHYQAVLVNDEKDPGPAMMDALVVED
                     LIKRLQAAKLTKPPS"
     gene            complement(1291..2220)
                     /locus_tag="PK362_03385"
     CDS             complement(1291..2220)
                     /locus_tag="PK362_03385"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC73917.1"
                     /translation="MTFAARFLRFALLTSFLFSLPWILQAAGAGTRPAAFLRLGVGAR
                     AAALGQASVALSDDAGAVYWNPGALSRLNGGQALFSHGSFLNNDLSYDAAAAGRALPG
                     VGVLGLLLQHASVGALPRLDGAAQETGSFSPADTAGTVALARTVQGHGLGISLKFVRS
                     KILNAASTVAGGVGYLSPPLWEGTLRVGVAADNWGGKLKYADRGETLPALFRVGVAYV
                     PRPGWEAVFDVERVQKTDPIYGGGLEYHRDFRGGRALLRGGYTTRTKDRGTAAGFSAG
                     AGVLFRKIGLDYAFLPDGDLGYTHLLNLSFLFP"
     gene            complement(2227..2715)
                     /locus_tag="PK362_03390"
     CDS             complement(2227..2715)
                     /locus_tag="PK362_03390"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC73918.1"
                     /translation="MKRVSWTLGVWLITAALFPFSSSAALLGENKRWTLSPKIGFSPF
                     TGVLGAEFRWEHLGLGAGYSAVGVKYYFSRRGSSPYVGPFFSKYRLDHNETIDGIDYD
                     RYTHREVGVSGGYQWQWKSRWNLEVGLKYSHLRKKWEKSPRWREEKGNFIGPAVAAGF
                     AF"
     gene            complement(2717..>3765)
                     /gene="trpB"
                     /locus_tag="PK362_03395"
     CDS             complement(2717..>3765)
                     /gene="trpB"
                     /locus_tag="PK362_03395"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNC73919.1"
                     /translation="NDLLAKFAGRPTPLLFAKNLTERLGGPRVYLKREDLCHTGAHKI
                     NNTLGQVLLAKRLGKKRVIAETGAGQHGVGTATACARLGLACEVYMGAEDIERQSLNV
                     FRMRSMGAKVIPVTTGSRTLKDAINEAMRDWVTNVRTTFYCLGSVVGPHPYPWLVREF
                     QSVIGREVLWQMKKAEGRNPDALVACVGGGSNAIGLFAPFYDHAKVKFYGVEAGGDGL
                     RTGRHAATLCTGTPGVLHGARTYLMQDENGQVLGTHSISAGLDYPAVGPEHAFYKDTG
                     RATYVAATDKEALAGFKLLNETEGLSGALESVHAVGFLPKLCRRMKKNQSVVVGLSGR
                     GDKDMVQLQQFLKN"
BASE COUNT          772 a         1160 c         1160 g          673 t
ORIGIN      
        1 ggtggtgcgc aggggcgtgc cgcacttgga gcaaaccttg aggttcttct ccagttcctt
       61 gttgtaaatg atttgctcgc acttgggaca cttggtccac aaaccgtgcg ggacggattt
      121 cttttttccg gattcgtcga cgggcatttt aatctcctat cggtttaacg cgtcgggggt
      181 ttcggccgct ccgcggccgc ccgacgtcct cgagctctgc gaaaggacgg cgcgcagggc
      241 gcgggccaaa ccggccaccg attctttgtt tcgtaagcgt tgaataaaag cggaacccac
      301 gatgacgccg tcggccaagg gcgccaggcg ccgggcgtcc cccgcggtcg ccaccccgaa
      361 acccacggcc acgggcacgg gcgatccccg gcgaagtcct tttaagaaag ccgggagatc
      421 cgaagaaaaa cctttgcggg ccccggtgac gccggtcacg gacaccgcgt aaagaaaccc
      481 gccgctttga cgggcgatcc atcgccgccg ggcggacggc gtggtcgggg ccaccaaatg
      541 aatcaagtgg atgccttgtt tcgtcagggc cgcccgcagg ggggcggctt cctcgggaat
      601 caaatcggga acaatgagcc cgtccacccc cgacgcccgg gcgtcccgag caaaccgggc
      661 gaacccccga cgggccacgg ggttcatata cgtcatgagc accaggggga catggtgttt
      721 ccgccgaaat ccccgggccc accgcaaaat tttatccggc gtggcaccgc cctccagagc
      781 cttctgggag gcgaattgaa tggtgggccc gtcggcgatg gggtcggaaa agggaacccc
      841 gatttccaga atatccacgc ccgcgcgcac caaatcctgg gcggcccggt ccatcccgga
      901 cagggtggga aaccctccgg tcaaataggc gatgaaagcg gaacgctttt ccttctttaa
      961 acccgcaaac acgccgtcaa ttcgatttaa aatacgttgc gacaaaagaa atccttcccc
     1021 ggatgaagag gcacggagac tattttaata tgaaaccgtc tataaaggaa ggacgaaaga
     1081 aaaacaggct gatttttaaa tgtgatttaa gaaggcggct tggtcaactt ggccgcttga
     1141 agccgtttta taagatcctc cacgaccagg gcgtccatca tcgccggccc cgggtccttc
     1201 tcgtcgttca ccaaaacggc ttggtagtga cggagagcca tcagaaggtg ggtgacctgc
     1261 cggtccgtga aagagaagtc cgccatgttc ctaaggaaaa aggaaagaca ggttcaggag
     1321 gtgggtgtag cccaagtcgc cgtccgggag aaaagcgtaa tccaatccga ttttcctaaa
     1381 aaggactccc gcgccggcgg agaaaccggc cgccgtgccc cggtctttgg ttcgcgtcgt
     1441 gtatccgccg cgcagtaaag cccggccgcc gcgaaaatcc cggtgatatt ccaacccgcc
     1501 accgtaaatc gggtcggtct tttggacccg ctcgacgtcg aaaacggcct cccagccggg
     1561 acggggaacg taggccaccc ccacccgaaa caaggccgga agggtttcgc cgcgatcggc
     1621 gtatttgagt ttccctcccc agttgtcggc ggcgacgccc acccgcaggg tgccttccca
     1681 aagaggcggg gacagatacc ccacgccgcc ggcgaccgtg gaggcggcgt ttagaatttt
     1741 acttcggacg aatttgagtg aaatccccag tccatgacct tgaacggtgc gggccagggc
     1801 gacggttccc gccgtgtccg ccggcgagaa gcttcccgtt tcctgggccg ccccgtccag
     1861 gcgcggcagg gcccccacgg aggcgtgctg caacaaaagg cccaggaccc cgaccccggg
     1921 aagagcccgg cccgcggcgg ccgcgtcgta ggacaagtcg ttgtttagaa aacttccatg
     1981 ggagaagagc gcttggcccc cgttcaaccg tgagagggcc ccgggattcc agtaaacggc
     2041 gccggcgtcg tccgacaagg ccacggaggc ttgccccaac gccgccgccc gggccccgac
     2101 cccgagacgg agaaaagcgg cgggacgggt tcccgccccg gcggcctgga gaatccatgg
     2161 gagggagaaa agaaaagacg tcaggagcgc gaagcgcaaa aaccgcgcgg cgaaagtcac
     2221 cggggcctaa aaagcgaatc cggcggcgac ggccggaccg atgaaattgc ccttttcctc
     2281 ccgccagcgg ggcgattttt cccacttctt ccgaaggtgg gaatatttaa gtcccacttc
     2341 caaattccat cgggatttcc actgccactg atatccgccg gaaacgccga cctctcgatg
     2401 ggtgtaccgg tcgtagtcga tgccgtcgat ggtctcgttg tggtccagtc ggtatttgga
     2461 aaaaaaaggc ccgacgtagg gggaggaacc gcgcctcgaa aaataatatt tcacgccgac
     2521 cgcggaatac cccgccccca gacccaggtg ctcccatcga aattccgccc caagaacacc
     2581 cgtaaagggc gagaaaccga tcttgggcga gagggtccaa cgcttgtttt ccccgagaag
     2641 ggcggccgag gaggaaaatg gaaaaagcgc cgcggttatc agccaaacgc ccaaggtcca
     2701 ggacactctt ttcattttaa ttcttcagga attgctggag ctgaaccatg tccttgtcgc
     2761 cccggccgga aaggccgacg acgacggatt ggtttttttt catccggcgg cagagtttcg
     2821 gcaggaatcc gacggcgtgg acggactcca gggcgccgga aagaccttcg gtttcgttca
     2881 gcaatttgaa acccgccagg gcttctttgt ccgtggcggc cacgtaggtg gcccgacccg
     2941 tgtctttgta aaaggcgtgt tcgggaccca ccgccgggta atccagcccg gccgaaatgg
     3001 agtgggtgcc cagaacctgg ccgttttcgt cctgcatcaa atacgtccgg gcgccgtgaa
     3061 gcacgccggg cgttcccgtg cacaaggtgg ccgcgtggcg gcccgttcgc aggccgtcgc
     3121 cgccggcctc cacgccgtaa aattttactt tcgcgtggtc gtaaaagggc gcgaagaggc
     3181 cgatggcgtt ggacccgccg ccgacgcagg ccaccagggc gtcgggattc cgcccctcgg
     3241 cttttttcat ttgccacagg acttcccggc cgatgacgct ttgaaactcc cgcaccagcc
     3301 aggggtaggg gtggggcccg acgacggacc cgagacaata aaacgtcgtt cgcacgttcg
     3361 tgacccagtc ccgcatggct tcgttgatgg cgtctttgag cgtgcgggat ccggtggtca
     3421 ccggaatgac cttggccccc atgctccgca tgcgaaacac gttgagcgat tggcgttcga
     3481 tgtcctcggc gcccatgtaa acttcacagg ccaggcccag ccgcgcgcag gcggtggcgg
     3541 tgccaacccc gtgctggccc gcgccggtct cggcgatgac gcgctttttc cccagccgct
     3601 tggccagaag gacctgcccg agcgtgttgt tgattttgtg ggcgccggtg tggcagaggt
     3661 cctcgcgctt caaatagacc cggggcccgc ccaggcgctc ggtcaaattt ttggcgaaaa
     3721 gaagcggggt cggccgtccg gcgaattttg ccaaaaggtc gttca
//
LOCUS       DAOQLS010000129         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12750_length_3725_cov_3.370300, whole genome shotgun sequence.
ACCESSION   DAOQLS010000129 DAOQLS010000000
VERSION     DAOQLS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12750_length_3725_cov_3.370300"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PK362_03400"
     CDS             complement(<1..625)
                     /locus_tag="PK362_03400"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNC73920.1"
                     /translation="MSFTGGLMSMMYSRRLLFGAVLLGPLSVSAFEGLVDYQMTSGEK
                     KPVSMSYKVKGDKIRAEFPSEKGRSGAMIMDSGSRTIWILSPEQRMAMKQTMAEPKSP
                     EAKAGATPRVEFKKTSRKETIAGRECTVYTFKETHSEGEVCNAEGLGNFMFSGFGGGK
                     REASGWEREIMGKGLFPLRVMSKNLKDGNTTTLLATRVETKSLPASDF"
     gene            complement(768..3200)
                     /locus_tag="PK362_03405"
     CDS             complement(768..3200)
                     /locus_tag="PK362_03405"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNC73921.1"
                     /translation="MTAEDFRGLIVRNLRTHSGRTPEFASKYDWYNAVALAVREQIYA
                     TENVAMARFKKKDTRLVAYLSAEFLLGPHLRNHIINMGLRAPVEEALKSLDQDFDDVM
                     AQETEPGLGNGGLGRLAACFLDSLATLGVPAAGYGIRYEFGIFGQVLRDGAQVEVTDK
                     WLKNGNPWEVQHPDVAFRVGFGGRTETVKENGGFKVRWHPDWVVRGVAYDTPISGYRA
                     KHAALLRLWKAEAVESFDFAAFNSGDYYRAVEQKMASENISKVLYPNDSSSQGKRLRL
                     EQQYFFVSCTLQDLIRINHLWGHPLKDFPKKFAVQLNDTHPAVAVPELMRLLMDVHGY
                     GWDDAWDMTRRTFSYTNHTLLPEALERWPLALFGALLPRHLEIIYEINRRFLAEVRLA
                     RPGDEDLLRRVSLIDESGERYVRMAHVAVVGGHAVNGVAALHTRLLREDLLKDFDALA
                     PGKIQNVTNGVTPRRWVVSSNPGLTKIISDAIGEKWIFRAEDELVQLEPFAADAAFRA
                     AWNRVKAENKNRLAARLKSATGLVVDPAALMDVQVKRIHEYKRQHLSVLHIVRRFLDL
                     KARPQSAPVPRTFLFGGKAAPGYFMAKLIIRFINAVGEAVNRDPAVSRIYRVAFFPDF
                     NVKNGEFIYPAAELSEQISTAGKEASGTGNMKFTLNGALTVGTLDGANVEIREAVGEE
                     NFFLFGHTVGEVGAALRNGYRGRDVYDRDPRVKEVVDSISSGFFSRGDPGVFRPLVDS
                     LMGADPYLTLADFSSYLAAQDRIDQTRRDPDDWSRRSILNVARAGRFSSDRSIREYAQ
                     NIWHVPLGEPDA"
     assembly_gap    3370..3379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3380..3613)
                     /locus_tag="PK362_03410"
     CDS             complement(<3380..3613)
                     /locus_tag="PK362_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73922.1"
                     /translation="MVFPPASVTSLQFTYADPAAAGDGPRIFPSPYRPGTDGSLTLAP
                     VPDGATITVYDLTGQTLARLPVSAGRAQWDGRDG"
BASE COUNT          676 a         1172 c         1167 g          700 t
ORIGIN      
        1 cgaaatccga ggcgggcagg gacttcgttt ccacccgcgt ggccaacagg gtcgtcgtgt
       61 ttccgtcctt aagatttttc gacatcaccc gcaggggaaa gaggcccttt cccatgattt
      121 cccgttccca accggaagcc tcccgtttcc cgccgccgaa cccgctgaac atgaaattcc
      181 ccaacccttc ggcgttgcaa acctcgcctt cggaatgggt ttccttaaac gtgtaaaccg
      241 tgcattcccg tccggcgatg gtttccttgc gggaggtttt tttgaattcg acgcgtggcg
      301 tggcgccggc tttcgcctcg ggcgatttgg gttcggccat ggtttgtttc atggccatgc
      361 gttgttccgg cgacaagatc caaatggtgc gtgaacccga atccataatc atggcgcccg
      421 accggccttt ttccgatggg aattcggcgc ggatcttgtc ccctttgact ttgtagctca
      481 tggaaacggg ctttttctcc ccgctggtca tttgataatc caccaaccct tcaaaagccg
      541 acaccgacaa gggcccgagc aacaccgctc cgaacaacaa acgccgcgaa tacatcatcg
      601 acatgagacc tcccgtgaat gacatcaacc atccgacacc ttattatagc gcccttaagc
      661 gccgaggggt aagggaagac tccgacttct taggggggcc cattgtcccc caggtgaaaa
      721 gaccgggggc ctgaccaccg acgtcgccgc cgttcacaca gggcgtgtca ggcgtcgggc
      781 tctccgaggg ggacgtgcca gatgttttgg gcgtattccc gaatggaccg gtccgaggag
      841 aaacgcccgg cccgggcgac gtttaaaatc gaccggcggg accagtcgtc cgggtcccgc
      901 cgggtttgat cgatccggtc ctgggccgcc aggtaggagg aaaaatccgc cagggtcaaa
      961 taggggtccg cccccatcag ggagtcgacg aggggccgga aaacccccgg atcgccccgg
     1021 gagaaaaaac cggaggaaat ggagtcgacg acctccttca cccgggggtc ccgatcgtaa
     1081 acgtcccggc cccggtaccc gtttcgaagg gcggccccca cctcccccac cgtgtgaccg
     1141 aacaggaaga aattttcctc ccccacggct tcccggatct ccacgttggc cccgtccagc
     1201 gtgcccacgg tcagggctcc gttcagggtg aatttcatgt tgccggtgcc ggaggcttcc
     1261 ttgcccgccg tggagatctg ttccgacaat tccgccgccg ggtaaatgaa ttccccgttt
     1321 ttgacgttga aatcgggaaa gaaggccacc cggtagatcc ggctcacggc ggggtcccgg
     1381 ttgacggcct cccccacggc gttgatgaaa cggatgatga gcttggccat gaagtacccc
     1441 ggcgccgcct ttcccccgaa gagaaatgtc cggggaaccg gagcgctttg ggggcgcgcc
     1501 ttgaggtcca aaaaccgccg gacgatgtga agaacgctca ggtgttgccg tttgtattcg
     1561 tgaatgcgtt tgacctgaac gtccatcaaa gccgccggat cgacgacgag gccggtcgcg
     1621 gattttaaac gcgccgcgag gcggttcttg ttctcggcct tgacccggtt ccaagccgcc
     1681 cgaaaggcgg cgtcggcggc gaagggttcc aattggacca gctcgtcctc ggcgcggaag
     1741 atccattttt cgccgatggc gtccgagatg attttcgtca aacccgggtt gctcgacacc
     1801 acccaccgcc ggggcgtcac gccgttggtc acgttttgga ttttcccggg cgccagcgcg
     1861 tcgaaatctt tcagaaggtc ctcgcgcagg agacgcgtgt gcagggccgc caccccgttc
     1921 accgcgtgcc cccccaccac ggccacgtgg gccatgcgca cataccgttc gccggattcg
     1981 tcgatcaggg acacccggcg caggaggtcc tcgtcccccg gccgggccaa acgcacttcc
     2041 gccaggaacc gccggttgat ttcgtagatg atttccaggt gccggggcag gagcgcgccg
     2101 aagagggcca ggggccagcg ttccaacgcc tcgggcaaca gggtgtggtt cgtgtaggaa
     2161 aaggttcgtc gcgtcatgtc ccaggcgtcg tcccacccgt aaccgtggac gtccatcagc
     2221 aggcgcatca attccggcac ggccacggcg gggtgggtgt cgttcaactg gacggcgaac
     2281 tttttgggga aatccttgag cgggtggccc cagaggtgat tgatgcggat caggtcctga
     2341 agggtgcagg acacgaagaa atactgctgt tccaggcgca ggcgcttccc ttgggaggag
     2401 gaatcgttcg ggtaaaggac cttggagatg ttttccgagg ccattttctg ctcgacggcc
     2461 cggtaatagt cgccgctgtt gaaggcggcg aaatcgaagg actccacggc ctcggccttc
     2521 cacaaccgca agagcgccgc gtgtttggcc cggtacccgc tgatgggggt gtcgtaggcc
     2581 acgccccgga ccacccagtc gggatgccaa cggactttaa atccgccgtt ctccttcacg
     2641 gtttcggtcc gcccgccgaa gccgacccga aaggccacgt ccgggtgttg gacttcccac
     2701 gggttgccgt ttttgagcca cttgtcggtg acttccacct gggcgccgtc ccgcaaaacc
     2761 tggccgaaaa tcccgaattc gtagcgaatg ccgtagcccg ccgccggaac gcccagggtc
     2821 gccagggaat ccagaaagca ggccgccagc cgcccgagac cgccgtttcc caagcccggc
     2881 tccgtttcct gggccatcac atcgtcgaaa tcctggtcca gggatttcag cgcctcttcc
     2941 accggcgcgc ggaggcccat gttgatgatg tggttgcgaa gatggggccc cagcagaaat
     3001 tccgccgaga gataggccac gaggcgcgtg tccttctttt tgaaccgcgc catggcgacg
     3061 ttttcggtgg cgtagatttg ttcccggacc gccagggcca cggcgttgta ccaatcgtat
     3121 ttggaggcga attcgggggt gcgtccgctg tgggtgcgga ggttgcggac gatcaatccg
     3181 cggaagtctt cggcggtcat gggcgccatc gagcgtctcc taacgggggt ttccccttcc
     3241 cattatcgac gcgccgcccg tttcgcgcag gggcgcgggg gttttaacgc tgcaagacga
     3301 ttttccgggt ccaggacccg ccgggggcgg cgattcggac gacatacacg ccgctgcccg
     3361 ccggtcggcn nnnnnnnnnt ccgtcccggc cgtcccattg agcccgcccc gccgacacgg
     3421 gcaagcgggc cagggtctgt ccggtcaaat cgtagacggt gatcgtggcg ccgtcgggca
     3481 ccggcgccag ggtcaacgac ccgtcggttc cgggacggta aggcgacgga aaaatccggg
     3541 gaccgtcgcc ggccgcggcc gggtccgcgt aggtgaattg aagcgacgtg accgaagcgg
     3601 gcggaaaaac cacggtgaag gattgggcgc cgtcggcttc cggggggtgg gcgcggaact
     3661 ccgcggggga aagctcccgg cgaaccaccg ccgctcgatc ctcaagctga aggacctcac
     3721 gcagg
//
LOCUS       DAOQLS010000130         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12875_length_3702_cov_4.163422, whole genome shotgun sequence.
ACCESSION   DAOQLS010000130 DAOQLS010000000
VERSION     DAOQLS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12875_length_3702_cov_4.163422"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="PK362_03415"
     CDS             complement(<1..1329)
                     /locus_tag="PK362_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC73923.1"
                     /translation="MTAGASSAPLVVAGAGLAGLSCAHHARGPVEVIDRESRPGGTAR
                     SFEIDGFTFDFTGHLLHLHHPYTTAWVKRLLKGNWVVCRRDARIFSNGVLTAYPFQAN
                     LAGLPPRVIRDCVEGVRRARAQWGDRPLAGAGPLNFRQWCDRLFGTGISKHFMIPYNE
                     KLWRIGVDDLTPEWCGNFVPVPTLAEVEAGARSAHRKAFGYNTTFLYPRTGGIQALPE
                     ALAKEVRGLRLGVSLESVDWKNRRVLLSTGEWRPYRRLVSTLPLPELLKRLTPFPAEL
                     REASERLRWTTVLCVNLGVARSKISQASWIYYPEKKFPFYRVGFPMNFTPHVVPRGCS
                     SMYVEVSCDPGREPTGPADRARLLSRVREGLVAAKILRRSDRIPVAHVVPIRYAYVVY
                     DRHRTESVNRIFHWLDRRAGADSIGRYGAWKYSFMEEALLDGKRAADADAL"
     gene            complement(1326..2351)
                     /locus_tag="PK362_03420"
     CDS             complement(1326..2351)
                     /locus_tag="PK362_03420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNC73924.1"
                     /translation="MIFVYACVFLVAFALTFLLTPAVRRVALRFNVMDRPVTPVKTHK
                     VPVPYLGGVGIFVGVVGSLAIVRGLTHFPTGTLHALRGLLWGGLVILALGLIDDLKKP
                     DGLTFYMKFLFQFAASALLIAFDIRIRFVQPVWLANVLTVLWVTGISNAINLIDIMDG
                     LAASQSFVAATAFLLIAIPTEAVYVNVAAVAVAGASLAFLPHNFSKQHKIFMGDAGSL
                     TLGFWLAGFSLGTEYSRLNQVGVFAPLLILGLPVYDTFFVSCLRLLQGKSPFLGSRDH
                     LAQKLRGLGLTGRQVVLVFAAVAATLSAAAFFLTLTPFYISVAVIGAVALLGLIVMLK
                     LYDVVAE"
     gene            complement(2348..>3702)
                     /locus_tag="PK362_03425"
     CDS             complement(2348..>3702)
                     /locus_tag="PK362_03425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC73925.1"
                     /translation="QFWNVAVAALTAWVLGLWGRSKSKEKETRYDLWLFLTAPCLLLL
                     SSETYVEIPLAFWTSLALWSATKGIENPRRAWWGLAGLFGGFAAGIKYTGVLTPALLF
                     LGALFWPRPRSGPDRWKDAAALGASGFSVFLPWLVKNAVFTGGNPVFPFLPSVFPASK
                     VFMFKESAKAYFAVLDEYKGTSGLLITLFKMPFQLATDATRFGGGFDVTGDLGWALPL
                     LLLPLGILAVRRKAGSDGFLLAYAAAHIALWACLRPVLRFLFPVFPLICLVAGRGLVA
                     LVDRYGPALRRTIVAVGGLFALSNAVLFYWVERVRDPFPVAFGLIDRETYLSQKLDYY
                     PWTRAMAALPAASRVLFVGDQRGYYCPRPYMAPMALLPPPLRDWSDAAENADVLRRRL
                     IEMGFTHLFFNRREAERLRGYRVLDLTPRGAAVFESMLQGLTVLGATPGATLFALGAP
                     "
BASE COUNT          891 a         1170 c         1123 g          518 t
ORIGIN      
        1 aagagcgtcc gcgtcggcgg cgcgcttccc gtccaacagg gcttcctcca tgaaagaata
       61 cttccaagcg ccgtatcggc cgatggaatc ggcccccgcg cgccggtcca accagtggaa
      121 aatccggttc accgattcgg ttcgatgccg gtcgtagacg acgtaggcgt aacggatggg
      181 gaccacgtgg gccacgggaa ttcggtccga ccgtcgaagg atcttcgcgg cgaccaagcc
      241 ctcccgcacc cgggacaaca ggcgcgcccg atccgcgggc cccgtcggct cccgaccggg
      301 atcgcaggac acctcgacat acatggacga gcaaccgcgg ggaaccacgt ggggcgtaaa
      361 attcatgggg aatcccacgc ggtaaaaagg gaacttcttc tcgggataat agatccagga
      421 ggcctgcgat attttcgacc gggccacgcc caggttcacg cacaacacgg tggtccatcg
      481 caggcgctcg gacgcttcgc gcagttccgc cggaaagggc gtcaaccgct tcaacagttc
      541 cggcaaaggc aacgtggaga ccaaccgccg ataggggcgc cattcccccg tggaaagaag
      601 aacgcggcga tttttccaat ccacggactc gagggaaacc cccaaacgga ggccgcgcac
      661 ttccttcgcc agggcttcgg gaagggcttg aattccgccg gtgcgcggat aaaggaacgt
      721 cgtgttgtag ccgaaggctt ttcggtgggc cgatcgggcg ccggcttcca cttcggccag
      781 cgtggggacg gggacgaagt tgccgcacca ctcgggcgtg aggtcgtcca cgccgatgcg
      841 ccagagcttt tcgttgtagg ggatcatgaa atgcttcgaa atgccggtcc cgaaaagccg
      901 gtcgcaccat tgacggaaat tcagaggccc cgcccccgcc aagggccggt ctccccattg
      961 cgcccgcgcc cgacgaacgc cttcgacgca atcccggatc acccgcggag gcaatcccgc
     1021 cagattggct tgaaaggggt aggccgtcag aacgccgttg gagaatatcc gcgcgtcccg
     1081 tcgacaaacc acccaattgc ctttcaatag gcgtttgacc caggcggtgg tgtacggatg
     1141 atgcaaatga aggaggtggc cggtgaaatc gaaggtgaac ccgtcgattt caaacgaacg
     1201 ggcggtcccg ccgggccggg attcgcgatc gatcacctcc accgggcccc gggcgtggtg
     1261 ggcgcaggac aaaccggcca gtccggcgcc ggccaccacc agcggagcgg aagacgcgcc
     1321 cgccgtcatt cggccaccac gtcgtagagt ttcagcatga cgatcagacc caggagcgcc
     1381 acggccccga tgaccgcgac ggagatataa aagggcgtga gcgtcaggaa aaaagcggcc
     1441 gcggagagcg tcgcggcgac ggcggcaaaa acgaggacga cttgccggcc cgtcagtccc
     1501 aagccgcgca gtttttgggc caaatgatcc cgcgaaccga ggaagggcga tttgccctgg
     1561 agcaaccgaa ggcaggaaac aaaaaacgtg tcgtacaccg gcaaccccag aatcaacagg
     1621 ggggcgaaca cgcccacctg attgagccgc gaatattccg tccccagaga aaagccggcc
     1681 aaccaaaaac ccagggtcag gcttccggca tcgcccatga agattttatg ctgctttgaa
     1741 aaattgtgcg gcagaaaggc caagctcgcg cccgccacgg cgaccgccgc gacgttgacg
     1801 tagacggcct ccgtgggaat ggcgatcaac aggaaggccg tggcggccac gaaggattgg
     1861 gaagccgcca ggccgtccat aatgtcgatc agattgatgg cgttcgatat gcccgtcacc
     1921 cacaacacgg tcagcacgtt cgccagccac accggctgca cgaaccgaat gcgaatatca
     1981 aaggcaatca gcaaggcgga cgcggcgaat tgaaaaagga atttcatata aaacgtcaac
     2041 ccgtccggtt ttttgaggtc gtcgatcaat cccaacgcca ggatcaccaa cccgccccac
     2101 agcaaccccc gcaacgcgtg gagcgtcccg gtgggaaaat gggtcagccc gcggacgatc
     2161 gccaaggacc cgacgacgcc gacaaaaatg ccgacgccgc ccagatacgg caccgggact
     2221 ttgtgggttt tgacgggagt gaccgggcgg tccatcacgt tgaaacgcag agcgacccgg
     2281 cgaaccgccg gcgttagaag aaaggtcaag gcgaaggcca cgaggaaaac gcaggcgtag
     2341 acgaaaatca cggagccccc agggcgaaca acgtcgcccc cggcgtcgcg cccaacaccg
     2401 tcaacccctg cagcatggat tcaaagacgg cggccccgcg cggcgtcaaa tccaaaaccc
     2461 gatagccgcg aagtcgctcg gcttcccgcc tgttgaagaa cagatgggtg aatcccattt
     2521 cgatcaaccg ccgacgaagg acgtcggcgt tctcggcggc gtccgaccaa tcccttaagg
     2581 gcggcggcag aagggccatg ggagccatat agggccgggg acaatagtat ccccgttgat
     2641 cgccgacaaa caagacccgg ctggcggcgg gaagggccgc catggcccgg gtccacggat
     2701 aataatccaa tttttgactc aaataggttt cacggtcgat caacccaaaa gccacgggaa
     2761 agggatcccg gacgcgctcg acccaataaa aaagaacagc gttcgaaagg gcaaagagcc
     2821 cccccacggc gacgatcgtc cgccgcaggg cgggaccgta ccggtcgacg agagcgacca
     2881 agccccgccc cgccaccagg caaatcaacg ggaagacggg gaacaggaat cggagaaccg
     2941 gtcgtaggca cgcccacaaa gcgatgtgcg ccgcggcgta ggccaggaga aacccgtcgc
     3001 tccccgcctt ccggcgcacg gccaggatcc cgaggggaag gagcaacaac ggcagggccc
     3061 aacccaaatc gccggtgaca tcgaaccctc caccgaaacg ggtggcgtcc gtcgccaatt
     3121 gaaacggcat tttaaacagc gtgatcagca acccgctcgt tcctttgtat tcgtccaaga
     3181 cggcgaaata ggccttcgcg gattccttaa acataaagac cttggaggcg ggaaaaacgg
     3241 acggcaaaaa aggaaacacg gggtttcccc ccgtaaacac cgcgttcttc accagccaag
     3301 ggagaaaaac ggaaaagccc gaggcgccca gggccgcggc gtctttccag cggtccggcc
     3361 cggaccgggg cctcggccag aagagcgccc ccagaaaaag aagcgcgggg gtcaggacgc
     3421 cggtgtattt aattccggcg gcaaacccgc cgaacaatcc ggccagcccc caccaggccc
     3481 gacgggggtt ttcaatcccc ttggtcgcgg accagagggc caacgacgtc caaaaggcca
     3541 ggggaatttc cacgtatgtt tcgctcgaaa gcaggagcaa gcacggggcc gtgaggaaaa
     3601 gccataaatc gtaacgcgtt tccttttctt ttgatttcga ccgtccccaa agcccaagga
     3661 cccacgccgt caacgccgcc accgccacgt tccaaaactg ag
//
LOCUS       DAOQLS010000131         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12880_length_3701_cov_4.452825, whole genome shotgun sequence.
ACCESSION   DAOQLS010000131 DAOQLS010000000
VERSION     DAOQLS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12880_length_3701_cov_4.452825"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..762
                     /locus_tag="PK362_03430"
     CDS             7..762
                     /locus_tag="PK362_03430"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNC73926.1"
                     /translation="MASRLIVGTGKYKTMELMEAALRASGADMVTVAIRRVNLNDRPA
                     DEFWRHLPKDMVILPNTAGCYNAADAVRVSRLAREALDTPLIKLEVLGDPKTLLPDAA
                     GLVEATKILVAEGFQVLAYTNDDPILAKRLEDLGVAAVMPLAAPIGSGRGIQNPIHIQ
                     FIREAVTSVPVIVDAGVGTASDAAVAMELGVDGVLMNTAIAEAQDPVRMAEAMRLGVD
                     AGRRAFLAGRMPRREYAQASSPVTGTVSIARPQ"
     gene            759..1823
                     /locus_tag="PK362_03435"
     CDS             759..1823
                     /locus_tag="PK362_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73927.1"
                     /translation="MKRVLFIGLTLGALLAAGRADAKPRSGLDGSAFLGKPSGARFLG
                     LGETGAALPGTPQAPMYNPAALDALTATQLGLDFDLANQSALSKDVILRASSLRGRKL
                     TYLGFAAPAKAVFYRPLADFDETTVTVATDPANNFIQSSLQVSQFGISASQKTEKGYS
                     VGLGLSYLTGRRGFALAASGQPPVLEIADGNGFALDWGFRDQQGPILYGLSILNFPGI
                     IYWDRYKADQLPVVGRAGLGFQPVPGVVFFSDYEKRFKSGAPKPHFWHFGLEIAPLPW
                     LALRSGALSSDFNDRNKTVYSYGLGLATAQKHSLDLSLRTEHLQDQRVNRYGLSLNFP
                     LPQEDASRPSAHSSRGAPPK"
     gene            complement(1873..2064)
                     /gene="rpmB"
                     /locus_tag="PK362_03440"
     CDS             complement(1873..2064)
                     /gene="rpmB"
                     /locus_tag="PK362_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNC73928.1"
                     /translation="MAYRCSVCDKGVSTGKTVSHSHRKTNRRFLPNLQRVKVATDGGP
                     RRQYVCTRCLRSGRVHKAA"
     gene            2150..>3701
                     /locus_tag="PK362_03445"
     CDS             2150..>3701
                     /locus_tag="PK362_03445"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC73929.1"
                     /translation="MTSPAALPAHDAPPHAAPLQYLKGVGPERSKALRSLGLNTVQDL
                     LFYLPRAHEDRRMVEDFRRVSPGGKAAVAGVVESFDVSSAGKDLSLGRASCRGAVGPF
                     EAVWFRRRSYRYDVFQSLSRQLVPGARLAVYGFPERGPRGWSLRVEDFEVAPADGAPL
                     LHLGRWTPVYELTERVNGRWLRGVVAAALERWADRVEDPLPEALRRAQDLAPLAESLR
                     AFHFPANEADRDRARRRLAFDEFFLIELALAEVRRRRHDGPPAVPCAPTRRLLTPFRE
                     RLGFEFTTAQKHVINEIFADMAAARPMSRLLMGDVGSGKTAVAFSALLLAIENGAQAA
                     LMAPTEILAEQHAQGAARWLEGLPLRWALLTGGRAAAQKKRDREALERGEIDLAIGTH
                     ALLEEGVAFRRLGAVVVDEQHRFGVAQRATLSAKGPAPHALLTTATPIPRTLALTLYG
                     DLSVSVLAQSPPGRPGIVTRWSPEPAAWDAVRRAVARGRQAYVVFPLVEMSERLDLRA
                     VLDGWKRLK"
BASE COUNT          585 a         1225 c         1217 g          674 t
ORIGIN      
        1 cgaaccctgg cctcgcgcct catcgtgggg accggcaagt acaaaaccat ggagctgatg
       61 gaagcggcgc tccgggcctc gggcgcggac atggtgaccg tggccatccg ccgtgtgaat
      121 ctcaacgatc ggccggcgga cgagttctgg cgccatttgc cgaaggacat ggtcatcctc
      181 cccaacaccg cgggttgcta caacgccgcc gacgccgtgc gcgtctcccg cctcgctcgg
      241 gaggccctgg acaccccgct gatcaagctc gaagtcctgg gcgaccccaa aacccttttg
      301 cccgacgcgg cgggtttggt cgaagccacc aaaatcctgg tggccgaggg ttttcaggtc
      361 ctggcgtaca cgaacgacga tcccattttg gcgaaacggc tggaggacct cggcgtggcc
      421 gcggtcatgc cgctggccgc gcccatcggg tccggccggg ggattcaaaa tcccatccac
      481 atccagttta tccgggaagc cgtgacctcg gtccccgtga tcgtggacgc cggggtgggc
      541 accgcctcgg acgcggcggt ggccatggag ctgggcgtcg acggcgtgct gatgaacacg
      601 gccatcgccg aagcccagga cccggttcgc atggccgagg ccatgcgcct cggggtcgac
      661 gcgggccgcc gggcctttct cgcggggcgc atgccccgtc gcgaatacgc ccaggcctct
      721 tcgcccgtga ccggcacggt gtcgatcgcg aggccccaat gaagcgcgtt cttttcatcg
      781 gcctgacgct gggggccctc ctcgcggccg ggcgcgccga cgccaagccc cggtcggggc
      841 tggacggatc ggctttcctc ggaaaaccct cgggggctcg ttttctgggc ttgggggaaa
      901 ccggggccgc ccttccggga acgccccagg cgccgatgta caatcccgcc gccttggacg
      961 ctctcaccgc cacccagttg gggttggatt tcgacctggc caatcaaagc gccctttcaa
     1021 aggacgtcat tcttcgggct tcctccctgc gggggcggaa actcacttat ttgggtttcg
     1081 cggccccggc gaaggccgtc ttttaccgcc ccctggcgga tttcgatgaa accacggtca
     1141 ccgtggccac ggacccggcg aacaatttca tccaaagctc cctgcaggtt tcccagttcg
     1201 gcatcagcgc gtcccaaaaa accgaaaagg gatacagcgt cggactgggt ctgtcctatc
     1261 tcaccggccg ccggggtttc gccctggccg cctccggaca gccgccggtg cttgaaatcg
     1321 ccgatggaaa cgggttcgcc ttggattggg ggttccggga ccagcaaggg ccaatcctgt
     1381 acggcctgtc aattttgaat tttccgggga tcatttattg ggaccgttac aaagccgatc
     1441 aattgccggt ggtgggaagg gccggattgg gatttcaacc cgtgccgggg gtcgttttct
     1501 tcagcgacta cgaaaagcgg ttcaaaagcg gagcgcccaa accgcatttc tggcactttg
     1561 ggttggaaat cgcaccgctt ccttggttgg cgctccgaag cggcgcccta agttccgatt
     1621 tcaacgaccg gaacaaaacc gtctacagtt acggtctggg gctggccacc gcccagaaac
     1681 acagtttgga tttgtccctg cggaccgagc atcttcagga tcaacgggtg aaccggtacg
     1741 ggttgtcgct gaatttcccg ttgccccagg aagacgcgtc ccgcccctcc gcccactcgt
     1801 cccggggggc gccgcccaaa taaaaaaacc cgggaccgaa gtcccgggtt tttttgaaaa
     1861 caatccgcgt cgttaggcgg ctttgtggac gcggccggac cgaaggcaac gggtgcaaac
     1921 gtactgacgg cggggaccgc cgtcggtggc gactttgacc cgctgaaggt tcggtaaaaa
     1981 gcggcgattg gttttgcggt gcgagtggct gacggttttg ccggtcgaaa cgcccttgtc
     2041 gcacacgctg caacggtaag ccattgacat ctccttggga agccccgcgg gggggcggtt
     2101 ccgtctactt aagacgaaga atgttaccaa tttttaccgc ggatttcaaa tgacctcacc
     2161 cgcggccctc cccgcccacg acgccccgcc ccacgccgcc ccccttcagt atttaaaggg
     2221 ggtcggtccc gaacgttcca aagccctgcg ttccttgggt ctgaacacgg ttcaggatct
     2281 ccttttttac ctgccccggg cccacgaaga ccgccgaatg gtcgaagatt tccgccgggt
     2341 ttccccggga gggaaagcgg ccgtcgccgg ggtggtcgaa tccttcgacg tgtcctccgc
     2401 cgggaaggac ctgagcctcg gtcgggcttc ctgccggggc gccgtcggcc ctttcgaggc
     2461 cgtgtggttt cgccggcgtt cctaccgcta cgatgtcttc cagagtttaa gccgacaatt
     2521 ggttccgggg gcgcgattgg ccgtctacgg gtttcccgag cgggggcccc gcggttggag
     2581 cctgcgggtg gaggatttcg aagtggcgcc ggccgacggg gcgcccctcc ttcacctggg
     2641 ccgctggacg cccgtctacg agttgacaga gagggtgaac ggtcggtggc ttcgcggcgt
     2701 cgtggcggcg gccctcgaac ggtgggcgga ccgggtggag gatcccctgc cggaggccct
     2761 gcgccgggcc caggacctgg ccccgttggc ggagagcctc cgggcgtttc atttccccgc
     2821 gaacgaagcg gaccgggacc gggcccgccg ccggctggcc ttcgacgaat ttttcctgat
     2881 cgagctggcc ctggccgagg ttcgccgtcg gcggcacgac gggccgccgg ccgtcccctg
     2941 cgcgccgacc cgccggttgt tgacgccttt ccgggaacgt ctggggtttg aattcaccac
     3001 ggcccagaag cacgtcatca acgaaatctt cgccgatatg gcggcggccc gccccatgag
     3061 ccgattgctc atgggggacg tggggtccgg gaaaacggcc gtggcttttt cggccctcct
     3121 gctggccatc gaaaacggcg cccaggcggc cctcatggcc ccgacggaaa tattggccga
     3181 acagcacgcc cagggcgccg cccgttggct ggagggactt cccctgcggt gggcgcttct
     3241 gaccggcggc cgcgccgcgg cccaaaaaaa gcgggaccgg gaagccctcg agcgcggcga
     3301 gatcgatctg gccatcggca cccacgcgct tttggaggaa ggcgtcgctt ttcgccggct
     3361 gggggccgtc gtcgtcgatg aacaacaccg attcggggtg gcccagcgag ccacgctgtc
     3421 ggccaagggc ccggcgcccc acgcgctgct caccaccgcg accccgatcc cccggaccct
     3481 ggccctcacg ctttacggcg atttgtcggt ttcggttctc gctcagtcgc ccccggggcg
     3541 gcccgggatc gtcacccgct ggtcgccgga gccggcggcc tgggacgcgg tgcgccgggc
     3601 ggtggcccgg gggcgccagg cctacgtcgt gtttcccctg gtggaaatgt cggagcgcct
     3661 cgaccttcgc gcggttttgg acggatggaa gcggctcaaa g
//
LOCUS       DAOQLS010000132         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12979_length_3683_cov_4.609978, whole genome shotgun sequence.
ACCESSION   DAOQLS010000132 DAOQLS010000000
VERSION     DAOQLS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12979_length_3683_cov_4.609978"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK362_03450"
     CDS             <1..375
                     /locus_tag="PK362_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC73930.1"
                     /translation="IESARETLKDIVAASAATRPVRVEDIQEVVADHFNLELKDMKSK
                     RRTDAIAFPRQIAMYLARTMTECSTTEIGDHFGGKDHTTVMHACNKIRGKMTSDPYFT
                     ALVNKITQQVKNHATLGDLRSK"
     gene            695..1834
                     /gene="dnaN"
                     /locus_tag="PK362_03455"
     CDS             695..1834
                     /gene="dnaN"
                     /locus_tag="PK362_03455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNC73931.1"
                     /translation="MSSPKKTDAALEIHCQKEELLKGVHLVQNAISPRSTLPVLSNVL
                     FESTDQGLRLSSTDLEVGIRCLVKADVKTLGSLTVPAKPLGEFLRTLDDGKDVSLKST
                     DGQKIEIRSGRDRCSLACLPKDDYPVLPEFNLEKAVSLNPAVLRDMIRKTAFAVSTDE
                     TRYVLNGVDFMVEKGQMTLVATDGRRLAFIQREIADKKASINAIIPTKAVNELARVLS
                     ADEKGAELLVGFTENQVTFQYKNVVIISRLIEGNFPNFEQVIPKSHDTRLRLKTKQIL
                     SATQRAAVGTLERGGSVRFALSAGRLQISASAQGRVEVESEVEVDYKGEPFAIAFNPA
                     YLVDVFKSLEADEIFLEFSTPLNPGVIHPVGDDNYKYVVMPMQVS"
     gene            1862..3004
                     /gene="recF"
                     /locus_tag="PK362_03460"
     CDS             1862..3004
                     /gene="recF"
                     /locus_tag="PK362_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNC73932.1"
                     /translation="MRLTHLGLRQFRNFARLDLAPAPGLNVLVGPNGAGKSNILESIA
                     VLATGQSHRGAEIRHWIQESKDELALVAQLEGEETIALELRQKRGRMRQVKFNNRPQA
                     RARDWAGRIPVVAFSPEDLDLVKGDPGVRRRALNDVLGQVSPEYADALARYQKLLEER
                     NAALRQVREGAAKANVLEPWDLALLKEGARLTVVRQRFSDEFAGRLQRRQQDLSLGRD
                     RVAMIYRPSFHVPSENEDEVAEANRRRLADLREGEIALGSTLIGPHRDDVEFRLGDEP
                     AKARASQGQTRTFALSWKWEERLYLKEKLAREPLCLLDDVFSELDPERRHQLTERLMA
                     GTQCFVTLTDLSTWGAFGIGDARIFEVRDGSVDHSTSVLDAPGERA"
     gene            3052..3339
                     /locus_tag="PK362_03465"
     CDS             3052..3339
                     /locus_tag="PK362_03465"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNC73933.1"
                     /translation="MIAAVLARSGYEPRHYALFDVWDRLLGPQAAKARAVGVKNGRLY
                     VDVDSSVRLHTLTLRKRELVKKMNGAFGEDAPLSDIIFRLGTTPFSDDTNA"
     gene            3332..>3683
                     /locus_tag="PK362_03470"
     CDS             3332..>3683
                     /locus_tag="PK362_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC73934.1"
                     /translation="MPKKTKTPATKNANPKRAADKAATTRRQRVATEALDKPAKNAAY
                     DSSSIQVLEGLEAVRRRPGMYIGPTDQRGLHHLVYEAVDNSIDEVLAGHATLVEVTIH
                     EDNSITVLDDGRGIP"
BASE COUNT          801 a         1213 c         1013 g          656 t
ORIGIN      
        1 atcgaatcgg cccgagaaac gctaaaggac atcgtggcgg cctcggcggc cacccgcccc
       61 gtccgcgtcg aagacattca ggaagtcgtg gcggaccatt tcaatttgga attgaaagac
      121 atgaaatcca aacgccgcac cgacgccatc gccttcccgc gccaaatcgc catgtatttg
      181 gcccggacca tgaccgagtg ctccacgacg gaaatcggcg accatttcgg cggcaaagac
      241 cacaccacgg tcatgcacgc ctgcaacaaa atccggggca aaatgacgtc ggatccctat
      301 ttcaccgccc tggtcaacaa aatcacccag caagtgaaaa atcacgccac gctgggcgat
      361 ctccgctcca aataattttt tgttttgtcg gcgtcgggcc cgttcgggga cggtcttttt
      421 ttatccgcgt tgacttcggg acaaggttcg ggataacccc tgaacaacct ccccgatcgg
      481 tggaacccgg gagggccttt ggaacaaccg ggacaacgct cgcggctttc ccacaggttg
      541 ttcgatcgtg aaaacaatcg agagtcgccg tccatcaaag gctttcgcgc cgtctaggaa
      601 attactatca ttaccccgaa cccggctcct acgacgactc ttatctttat agaccaatag
      661 aaagaggatg gccttgaata actcaagcac tgttatgtcc tcgcccaaaa aaaccgacgc
      721 cgctctggaa attcattgcc aaaaagagga actcctaaaa ggcgttcatc tggttcaaaa
      781 cgccatttcg ccccggagca ccctgcccgt cttgtccaac gttctttttg aatccaccga
      841 tcaaggcctt cgcttgtcct ccacggattt ggaagtcggg atccgttgcc tcgtgaaagc
      901 ggacgtgaaa accctgggca gcctgaccgt cccggccaag cctctggggg aatttctccg
      961 aaccttggac gacggcaagg acgtctccct caagagtacc gacggccaaa aaatcgaaat
     1021 ccgctccggc cgtgaccgtt gctccctggc ctgcctgccc aaggacgatt accccgtcct
     1081 tcccgaattc aacttggaaa aagccgtgtc cctgaacccg gccgttctgc gcgacatgat
     1141 ccgcaaaacg gccttcgcgg tttccacgga cgagacccgt tacgtcttga acggcgtgga
     1201 tttcatggtg gaaaagggtc aaatgaccct ggtcgccacc gacggccgcc gactggcctt
     1261 catccagcgc gaaatcgccg ataaaaaagc ctccatcaac gccattatcc ccaccaaagc
     1321 ggtcaacgaa ttggcccggg tgctgagcgc ggacgaaaaa ggggccgagc tgttggtcgg
     1381 cttcaccgag aaccaggtga cgtttcaata caaaaacgtg gtcattattt cccgcttgat
     1441 cgaaggcaat ttccccaatt tcgaacaggt gattcccaaa tcccacgaca cccgccttcg
     1501 actgaaaaca aagcaaatcc tttcggccac ccaacgcgcc gccgtgggta ccctggaacg
     1561 gggcgggtcc gtgcgtttcg ctctttcggc cgggcgcctg caaatctccg cctcggccca
     1621 gggccgcgtg gaagtggaat ccgaggtgga ggtcgactac aaaggcgagc ccttcgccat
     1681 cgccttcaat cccgcttacc tggtggacgt ttttaaatcc ctggaagccg acgaaatctt
     1741 tttggaattc agcaccccgc tcaaccccgg cgttatccac cccgtgggcg acgacaacta
     1801 caaatacgtc gttatgccca tgcaggtgtc ctaacccggg cctttccccg cccttcccca
     1861 cgtgcgtctg acccacctgg gcctccggca attccggaac ttcgcccggc tggacctcgc
     1921 gccggctccc ggactgaacg tcttggtcgg ccccaacggg gccggtaaat cgaatatttt
     1981 ggagtccatc gccgttctgg ccacgggtca aagccatcgc ggggccgaaa tccgccattg
     2041 gatccaggaa agcaaagacg agctcgccct ggtcgcccaa ctggaaggcg aagaaaccat
     2101 cgccctggaa ctccgccaaa aacggggacg gatgcgacag gtgaaattca acaaccgccc
     2161 ccaggcccgg gcccgggatt gggccggccg gattcccgtg gtggcctttt cgcccgagga
     2221 tttggatttg gtcaaagggg accccggcgt ccgccgccgg gccttaaacg acgttttggg
     2281 ccaggtgagc cccgaatacg ccgacgccct ggcccggtac cagaaactct tggaggaacg
     2341 gaacgccgcc ctgcgccagg tgcgggaggg cgccgccaaa gccaacgtgc tggaaccctg
     2401 ggatttggcg cttctgaaag agggcgcccg cctcaccgtg gtccgacagc gcttttccga
     2461 tgaattcgcc ggccgactgc aacgccggca gcaggacctt tccctcggac gggaccgggt
     2521 ggccatgatt taccggccct cgttccacgt tccctccgaa aacgaggacg aagtcgccga
     2581 ggccaaccgc cgacggctgg cggacctccg ggagggggaa atcgccctgg gatccacttt
     2641 gatcggcccc caccgggacg acgtggaatt ccgcctgggg gacgaaccgg cgaaggcccg
     2701 ggcctcccag ggccagaccc ggaccttcgc gctctcctgg aaatgggagg aacggcttta
     2761 tctcaaggaa aaactggccc gggagcccct ctgtttgctg gacgacgttt tctcggaact
     2821 ggaccccgaa cggcgccacc aattaaccga acgcctgatg gcgggcaccc aatgctttgt
     2881 cacgctgacc gatttgtcca cctggggcgc cttcggcatc ggcgacgcgc gcattttcga
     2941 ggttcgggac ggaagcgtgg atcattccac cagcgtcctg gacgcccccg gggagcgcgc
     3001 ctgagccccg tccgcaaacc caaggaaaag gtcctccgcc ccgccaaggc cgtgatcgcg
     3061 gcggtgttgg cccgttccgg gtacgaaccg cgccactacg cccttttcga cgtgtgggac
     3121 cgtctcctgg gtccccaggc ggccaaggcc cgggccgtgg gcgtgaaaaa cggccggcta
     3181 tacgtggacg tggacagttc ggtccgcctg cacaccctga ccctgcgcaa gcgggagctg
     3241 gtcaaaaaaa tgaacggagc ctttggcgaa gacgcccccc tttctgatat aattttccgt
     3301 ctagggacga cccccttttc cgacgacacc aatgcctaag aaaaccaaaa ccccggcgac
     3361 caaaaacgcc aatccgaaac gggccgccga caaagcggcg acaaccagga ggcaacgagt
     3421 ggccaccgaa gcgttagaca aacccgcgaa aaacgcggct tacgattctt catccatcca
     3481 agtgctggag ggcctggagg ccgtccgccg ccgccccggc atgtacatcg gacccaccga
     3541 ccagcggggc cttcaccatc tcgtgtacga agccgtcgac aactcgatcg acgaagtctt
     3601 ggccggccac gccaccctgg tggaagtgac catccacgaa gacaactcca tcaccgtcct
     3661 ggacgacggc cgcggcattc ccg
//
LOCUS       DAOQLS010000133         3682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_12986_length_3682_cov_3.714364, whole genome shotgun sequence.
ACCESSION   DAOQLS010000133 DAOQLS010000000
VERSION     DAOQLS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12986_length_3682_cov_3.714364"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PK362_03475"
     CDS             <1..664
                     /locus_tag="PK362_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC73935.1"
                     /translation="GGERWVLVESVYSMDGDRAPLVELSAVCRRRGARLYVDEAHGTG
                     VWGPQGRGVVNDLGLEKEVDVCMGTLSKALGAQGGFVGGSRALVDWLQNKARAYIYST
                     ALAPASAGAALAALSIAREDDAGRKKLFALSARLRAGLGLEGDGPIVPVVVGETSAAL
                     DLSAALWDAGFLAPAVRPPTVPEGTARVRFSLTAGHTEEQIDGALAVVRRWREKNAVE
                     VS"
     gene            661..1347
                     /gene="bioD"
                     /locus_tag="PK362_03480"
     CDS             661..1347
                     /gene="bioD"
                     /locus_tag="PK362_03480"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNC73936.1"
                     /translation="MKGVFVTATDTGVGKTHVAAGLARSLRRAGKNVGVFKPFACGSW
                     ADTDALIRAAGSGQTRAEVTPFYYSRPLAPIVKFGLGRTGTVAGRRAWTNVVASWKEL
                     TKRFDVVVVEGAGGALVPFHGKLTVADAARSFGLPVWVVARPGLGTLNHTLLTLEALE
                     RRGLKVRRVVVSGYRGKNEAERSNPRLLAVLGRRPVTVLPWGKSSLKSGAPARRIAAA
                     WRTDIFGGRP"
     gene            1344..2798
                     /gene="bioA"
                     /locus_tag="PK362_03485"
     CDS             1344..2798
                     /gene="bioA"
                     /locus_tag="PK362_03485"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNC73937.1"
                     /translation="MTSAKILGQWDKKYLWHPFTQQAEWNKEEPTIVQSARGVRLKDQ
                     HGREYIDGVSSLWVTVHGHREPALDRAVRRQLGRVAHSTFLGLTHEPAIRLGKALADI
                     APKGLSRVFYSDNGATAVEVALKMAFQYRVHTEGPSTRRREYLALSHSYHGDTLGAVS
                     VGGIAHFQKVFRPLLFRARFAPSPHCDRCPFRKKPSTPRLRTGDERVRGPAPRPGDFR
                     AETGCRWECLSGAEKILKERRSRLVAAIVEPLVQAAVGLWVMPPGYLRGFARLCRKHG
                     VPLIADEVATGFGRTGTMFAVQQEGVVPDFLCVAKSLTGGYLPLAATLTSDKIFRAFL
                     GRYDQFKSFFHGHTYTANPLACAVALENLRLLKKKNWPRALAPLRRNLALFLKDIGDH
                     PRVGHVRQMGLMAGIELVADRKTGRRFPPGDRVGYRVCQAARKRGLWIRPLGDTLVIL
                     PPLGIGRVDLHRLTEAVRGALGEVLPPGDGTSFC"
     gene            2810..3523
                     /locus_tag="PK362_03490"
     CDS             2810..3523
                     /locus_tag="PK362_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNC73938.1"
                     /translation="MRRFWKLLAVAAIPGSLWAGRPAAKFDQVYADFGILPQGSTVQF
                     NYGFRNVGNEPLRIESVQTSCGCTAAAPENPVIAPGETGIIHVTFDSRGKMGETIKQI
                     RVRTNDPKSPMSFLTLAGKIVESKHPEMTDTRNIFKGACRECHVDRGEKRLGEELYMA
                     DCAMCHESQSRGGPALGPASETLALLPEAHLKSRITDGVPDTSMPAYGVKKGGPLSGG
                     QIRSLVHYLKSLNSRGGRQ"
BASE COUNT          602 a         1151 c         1273 g          656 t
ORIGIN      
        1 cgggggggag cgttgggtgt tggtggaaag cgtttattcc atggacggcg accgggcccc
       61 cctggtggaa ttgtcggcgg tttgccgacg gcgcggggcc cggctttacg tcgacgaggc
      121 tcacggcacg ggcgtctggg gtccccaggg gcggggcgtc gtgaacgatt tgggattgga
      181 aaaagaagtg gacgtctgca tgggcacgct ttccaaggcc ctgggcgccc aggggggatt
      241 cgtgggcggt tcccgggcgc tcgtcgattg gcttcaaaac aaggcccggg cctatattta
      301 ttcgacggcc ctggcccccg cctccgccgg cgcggccctg gccgccctct ccatcgcgcg
      361 ggaagacgac gccgggcgga aaaagctgtt cgccctttcg gcccgtctgc gggcggggct
      421 ggggttggaa ggggacgggc ccatcgtccc cgtcgtggtg ggggaaacgt cggcggccct
      481 ggatctttcg gccgctctct gggacgcggg gtttttggcc cccgccgttc ggccgcccac
      541 cgttcccgag ggcacggccc gggtgcggtt ttccctcacg gccggtcaca cggaggaaca
      601 gatcgacggg gccctggcgg tggtccgtcg atggcgggaa aaaaacgccg tggaggtttc
      661 gtgaaaggcg tgtttgtgac ggccaccgac accggcgtgg ggaaaaccca cgtggcggcg
      721 gggctggccc gctcccttcg gcgggccggc aaaaacgtcg gggtcttcaa acccttcgcc
      781 tgcggctcct gggcggacac cgacgctctg atccgcgcgg cgggctccgg ccaaacccgg
      841 gcggaggtca ccccctttta ttattcccgg cccctggcgc ccatcgtgaa attcggtttg
      901 gggcggaccg gaaccgtcgc cgggcggcgg gcctggacaa acgtggtggc gtcctggaag
      961 gaactcacca aacggttcga cgtcgtggtc gtggaagggg ccgggggcgc gctggtccct
     1021 ttccatggga aattgacggt ggctgacgcc gcgcgctctt tcggcctgcc cgtctgggtg
     1081 gtggcccgtc cgggcctggg cacgttgaac cacacgttgt tgaccctgga agccctggag
     1141 cgtcggggcc tgaaggtccg ccgggtggtg gtgagcggat accgggggaa aaacgaagcc
     1201 gagcgttcca acccccggct tttggcggtc ctgggtcgtc ggccggtgac ggttcttccc
     1261 tggggaaaat cctccctgaa atcgggcgcg ccggcccgcc gaattgcggc ggcctggcga
     1321 acggatattt ttggaggacg gccgtgacct ccgccaagat cctcggccaa tgggacaaga
     1381 aatacctttg gcatcccttc acccagcagg ccgagtggaa caaggaagag ccgaccatcg
     1441 ttcaaagcgc ccggggcgta cggttgaagg accagcacgg tcgggaatac atcgacggcg
     1501 tttcctccct ctgggtcacg gtccacggcc accgggaacc cgccctggac cgggcggttc
     1561 gccgccaatt gggccgggtg gcccacagca cctttttggg gctcacccac gagcccgcca
     1621 ttcgactggg aaaggccctg gccgacatcg cgccgaaggg actttcccgg gtgttttatt
     1681 cggacaacgg ggccacggcg gtggaagtcg ccctcaagat ggcttttcaa taccgggtgc
     1741 acaccgaggg tccctcaacg cgtcggcggg aatacctggc cctgtcccat tcctaccacg
     1801 gggataccct gggggcggtg tccgtgggcg gcatcgccca ttttcaaaaa gtgttccggc
     1861 cgctgctctt tcgggcgcgg tttgcgccct cgccccactg cgaccggtgt ccctttcgga
     1921 aaaagccgtc gacgccccgg ttgcgcaccg gcgacgagcg cgtccggggc cccgcgcccc
     1981 ggccgggcga ttttcgggcg gagacgggct gccggtggga atgcctgtcg ggggcggaaa
     2041 aaattttaaa ggagcggcgg tcccggctgg tggcggccat cgtcgagccc ctcgtgcagg
     2101 ccgccgtggg cctgtgggtc atgcccccgg gctatctgcg ggggttcgcc cgcctttgcc
     2161 ggaaacacgg ggtgcccttg atcgccgacg aagtggccac ggggttcggc cgtacgggaa
     2221 cgatgttcgc cgtccaacag gagggcgtgg tgcccgattt cctctgcgtg gccaagtccc
     2281 tgacgggggg ctatctgccc ctggcggcca cgctgacgtc ggacaagatt ttccgggcct
     2341 tcctggggcg ctacgaccaa ttcaaaagtt ttttccacgg ccacacctac accgcgaacc
     2401 ccctggcctg cgccgtcgct ctggaaaacc tgcgcctctt gaaaaagaaa aactggcccc
     2461 gggccctcgc cccccttcgg cggaatttgg cgcttttcct gaaagacata ggggaccatc
     2521 cccgggtggg acacgtaaga cagatgggac tcatggccgg gatcgaattg gtggccgatc
     2581 gaaagaccgg gcgtcgtttc ccgcccgggg atcgggtggg ataccgggtg tgccaagccg
     2641 cccgaaagcg gggactttgg atccgccccc tgggggacac gctggtcatt cttccgccct
     2701 tgggcatcgg ccgcgttgat cttcaccgat tgaccgaagc ggttcgaggg gccctgggcg
     2761 aggtcctccc gcccggcgac gggacctcat tttgctaaac tctccgtcca tgaggcgatt
     2821 ttggaaactc ctcgcggtgg ccgcgattcc cggttccctt tgggcggggc gccccgcggc
     2881 caaattcgac caggtctacg ctgattttgg aatcctgccc caggggtcca cggtccagtt
     2941 taattacggt tttcgaaacg tcggcaacga acccctgcgg atcgaatcgg tgcagacttc
     3001 ctgcggttgc acggcggccg caccggagaa tccggtgatc gcccccggtg aaacggggat
     3061 cattcacgtg accttcgact cccgcggcaa aatgggggag acgatcaaac agatccgggt
     3121 gcgcaccaac gaccccaagt cgcccatgtc gtttttgacg ttggccggaa aaatcgtgga
     3181 gtccaaacac ccggaaatga cggacacccg gaatattttc aaaggggcct gccgggaatg
     3241 ccacgtggac cgcggcgaga agcgcctggg cgaggaactc tatatggcgg attgcgccat
     3301 gtgccacgaa agtcagagcc gtggggggcc cgctttgggc cccgcgtccg aaacgctggc
     3361 gctcctcccg gaagcccatt tgaagtcgcg aatcaccgac ggggtgcccg acacctcgat
     3421 gcccgcctac ggcgtgaaaa aaggcgggcc cctgagcggc ggacaaatcc ggtcgctggt
     3481 ccactatttg aaatcgttaa attcccgagg agggaggcaa tgacatgcgg aaacacacgg
     3541 ggtggttgtt gggcggggcg ctgctgatgg ccgccggcgc gaaggcggag ggttggcgtt
     3601 cccaggccgg cacctacgcg gtcctggaca cgagcgcggg ggaaatcgtt tttcgtcttt
     3661 tgacggacaa ggccccggag ac
//
LOCUS       DAOQLS010000134         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13034_length_3676_cov_4.568075, whole genome shotgun sequence.
ACCESSION   DAOQLS010000134 DAOQLS010000000
VERSION     DAOQLS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13034_length_3676_cov_4.568075"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PK362_03495"
     CDS             <1..326
                     /locus_tag="PK362_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73939.1"
                     /translation="YLMPADPDLPSRINKEMVAVEPMEAEDIDEVRNLLKKHLLYTTS
                     TVAKHVLEKGPEYIKTFLKIMPKDYKRMIQAIKQVHAAGLTGDAAIMAAFEENARDLA
                     RVGGN"
     gene            328..747
                     /locus_tag="PK362_03500"
     CDS             328..747
                     /locus_tag="PK362_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC73940.1"
                     /translation="MASLVLEHKPAKSTQARQHFLHKLTVETDASDVYADLRAGVDAF
                     VVVDPRSAEAYAKGHVPGALNLPYREIDRDAVSARLPQGKVYVLCCAGIGCNASAKAA
                     IRLAAFGCAIKELVGGLEWWQKSGYPVQTGAEPGRLE"
     gene            825..2312
                     /locus_tag="PK362_03505"
     CDS             825..2312
                     /locus_tag="PK362_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNC73941.1"
                     /translation="MAKPTGFMKFTRELPKDADPASRLRHYKEFHEHLPEESLRKQGA
                     RCMDCGIPFCHTGNLVAGMASGCPINNLIPEWNDLVFRGLWHEALERLHKTNNFPEFT
                     GRVCPAPCEGSCVLGISEPPVTIKNMEVSIIDKAFAEGWVTPEVPAKRTGKKVAVVGS
                     GPAGLACAAQLNRAGHTVTVFERADRVGGLLMYGIPNMKLDKAVVKRRVDLLAKEGVT
                     FVTGTEVGKNYPAENLRKEFDAVALCTGATAPRDLPVPGRELKGVHFAMEFLHQNTKS
                     LLDSSHKDGKFISAAGKDVIVIGGGDTGTDCVGTALRHGCKSLVQFEILPRPPDARAA
                     DNPWPQWPKVYKMDYGQEEAAAVFGEDPRRYLISTKKIVSGPNAKRVKEIHTVQIEWA
                     KDAQGRFVPKEIPGSEKVWPAQLVLLAMGFLGPEAELLKALGVAQDERSNVKAEHGKF
                     ATSLPGVFAAGDARRGQSLVVWAINEGRGAAREIDRFLMGASDLP"
     gene            2320..>3676
                     /locus_tag="PK362_03510"
     CDS             2320..>3676
                     /locus_tag="PK362_03510"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNC73942.1"
                     /translation="MPIAPPRRPDRASRGFDPRPYRPFLGAVSPKGLVEIYAEESFRR
                     SLRWEDGRLDEITQSESAGVGLRWLDGAHTRFVAVDARRPASAGLSPAEARALRRLAP
                     GPLRLLRAPLAPTTLRTHSVRRDPATASLSQKAGWLALAEKAARAEGPLRQVSLTYGE
                     LQKTVGLANSVGEVFAEERTYLTFVVQVTAEKNGVLQTGYEVAAGVGGLEILDRPGLE
                     ELARLAARRALAKLEAPSAPAGEMAVVISSQAGGTLIHEAIGHALEADSVQDGSSPHF
                     AGKIGKVVANEKVTVMDDPTRDGHRGSFHRDDEGTPAERTVLVEGGVLKTYLYDRRTA
                     AAAGRVSNGHGRRESYAHKPIPRMSNTFIAPGADDPEAIRRELRRGLWVTRMGGGQVN
                     TATGDFVFEVEEGFWVEDGRVKHPVRGANLLGNGPDVLRSIDRVGSDLGWSVGTCGKE
                     GQ"
BASE COUNT          688 a         1232 c         1186 g          570 t
ORIGIN      
        1 cctacctgat gccggccgac ccggacctgc cgtcccgcat caacaaggaa atggtggcgg
       61 tggaacccat ggaggccgag gacatcgacg aggtccgaaa ccttcttaaa aaacacctgc
      121 tttacacgac gagcaccgtg gccaagcacg tgttggaaaa aggcccggag tacatcaaga
      181 ccttcctcaa gatcatgccc aaggattaca agcgcatgat ccaagcgatc aagcaggtgc
      241 acgcggcggg cctgaccggc gacgcggcca tcatggcggc ctttgaagaa aacgcgcggg
      301 atctcgcccg cgtggggggg aactgacgtg gcgtcgctcg ttctcgaaca caagccggcc
      361 aaatcgaccc aggcccggca gcattttctg cacaaattga cggtggaaac cgacgcctcg
      421 gacgtatacg ccgaccttcg ggcgggcgtc gacgccttcg tggtggtgga cccccgatcg
      481 gcggaagcct acgcgaaggg ccacgtgccc ggagccctca acctgccgta ccgggaaatc
      541 gatcgggacg cggtgtcggc ccgcctgccc caggggaaag tgtatgtgct ttgttgcgcc
      601 ggcatcgggt gcaacgcgtc ggccaaggcc gccatccgat tggcggcctt cgggtgcgcc
      661 attaaggaat tggtgggcgg tttggagtgg tggcaaaaaa gcggctatcc cgttcagacc
      721 ggggccgagc ctgggagact ggaatgaacc ctgtgaacga ttccacggtg gaaaaaccga
      781 gcaccaacgg ccacggcgcc ccgcccgcgc cgaaacccct cggggtggcg aagcccacgg
      841 gcttcatgaa gttcacccgg gagctaccca aagacgcgga cccggcgtcg cgcctccggc
      901 attacaagga atttcacgaa cacctgcccg aggagtccct ccgcaagcag ggggcccggt
      961 gcatggactg cggcattccc ttctgccaca ccggaaacct cgtggccggc atggcctccg
     1021 gctgtcccat caataatttg atcccggaat ggaacgacct ggtgttccgc gggttgtggc
     1081 acgaggcgct ggaacgcctg cacaagacca acaatttccc cgaattcacg ggccgggtct
     1141 gccccgcccc ctgcgaaggg tcctgcgttc tgggcatcag cgaacccccc gtgacgatta
     1201 aaaacatgga agtgtccatt atcgacaaag ccttcgccga agggtgggtc acccccgagg
     1261 ttccggccaa gcgaacgggt aaaaaagtcg cggtggtggg gtccggtccg gcgggcctgg
     1321 cctgcgcggc gcagctcaac cgggcgggcc acacggtcac cgtgtttgaa cgggccgatc
     1381 gggtgggcgg attgctcatg tacggcattc ccaacatgaa gctcgacaaa gccgtcgtca
     1441 agcggcgggt cgaccttttg gccaaggaag gcgtgacctt cgtcacgggc accgaggtgg
     1501 ggaaaaacta ccccgccgaa aatcttcgca aagagttcga cgccgtggcc ctgtgcaccg
     1561 gcgcaacggc cccccgggac ctccccgtgc cggggcggga gctcaagggc gtccacttcg
     1621 ccatggagtt cctccatcag aacaccaaga gcctcctgga ttcgagtcac aaggacggaa
     1681 aattcatatc ggcggccggc aaggacgtga tcgtcatcgg cggcggcgac acgggcaccg
     1741 actgcgtggg gaccgccctc cgccacgggt gcaagagctt ggtccagttt gagattttgc
     1801 cccgaccgcc ggacgcccgg gccgccgaca acccctggcc gcaatggccg aaggtctaca
     1861 aaatggacta cggccaggag gaggccgccg cggttttcgg ggaggacccc cggcgctatt
     1921 tgatctccac caaaaaaatc gtcagcgggc ccaacgcgaa gcgcgtgaag gaaatccaca
     1981 cggtccaaat cgaatgggcg aaagacgccc aaggccgttt cgtccccaag gaaattcccg
     2041 ggtccgaaaa agtttggccc gcccagctgg tgctcttggc catggggttc ctcgggccgg
     2101 aggccgaact gctcaaggcc ctgggcgtcg cccaggacga acgttccaac gtgaaggcgg
     2161 agcacgggaa attcgccacc agcctgcccg gggtcttcgc cgccggcgac gcccgacggg
     2221 gccaaagcct cgtcgtctgg gccatcaacg aaggccgcgg cgccgcccgc gaaatcgacc
     2281 gctttttgat gggcgcgtcg gacctcccct aacggatcca tgcccatcgc cccgccgcgt
     2341 cgtcccgacc gcgcgtcccg ggggtttgat cctcgcccct accgtccttt cctcggcgcg
     2401 gtttctccga agggattggt cgagatttac gccgaggaat ccttccgccg ctccctgcgg
     2461 tgggaggacg gccgtctcga tgaaattacc caatccgaaa gcgccggcgt cggcctccgc
     2521 tggttggacg gcgcccacac ccgattcgtg gccgtcgatg cccggcggcc cgcgtccgcc
     2581 gggctctcgc ccgccgaggc ccgcgccctt cggcgcctgg cccccgggcc cctccgcctc
     2641 ctccgggccc cgttggcccc cacgacgctc cgaacccatt cggttcgccg ggacccggcc
     2701 accgcttccc tttctcaaaa agccggctgg ctcgccctgg ccgaaaaggc cgcccgcgcc
     2761 gaaggccccc tgcgccaggt ttccctcact tacggcgaac tccaaaaaac cgttggtctt
     2821 gccaattcgg tcggcgaggt gttcgccgaa gagcggacgt atctcacctt tgtcgtccag
     2881 gtgacggccg aaaagaacgg cgttcttcag accgggtacg aggtggcggc cggggtgggg
     2941 ggattggaaa ttttggatcg gcccgggttg gaagagttgg cgcgcctggc ggcgcgccgg
     3001 gccctcgcca aactcgaggc gccttccgcc cccgcgggcg aaatggcggt ggtgatctcc
     3061 tcccaggcgg gcggcacgtt gatccacgag gccatcggcc acgctctgga ggccgacagc
     3121 gtccaggacg gaagctcgcc ccatttcgcc ggtaaaatcg gaaaagtcgt ggccaacgag
     3181 aaggtcacgg tcatggacga tcccacccgg gacggccatc gggggtcctt tcatcgggac
     3241 gacgaaggca cccccgccga acggaccgtc ctcgtggagg ggggcgttct caaaacgtat
     3301 ctgtacgacc ggcgcaccgc cgccgcggcg gggcgcgttt ccaacggcca cggccggcgg
     3361 gaatcctacg cccacaagcc cattccccgg atgtccaaca ccttcatcgc ccccggcgcg
     3421 gacgatcccg aagccatccg acgggaactt cgccggggcc tctgggtcac gcgcatgggc
     3481 ggcggccagg tgaacacggc cacgggcgat ttcgttttcg aagtggagga aggtttctgg
     3541 gtcgaggacg gccgggtgaa gcatcccgtc cggggcgcca acctgctcgg caacggcccc
     3601 gacgtcctac gaagcatcga ccgggtgggg tcggacctcg gatggtccgt gggcacctgc
     3661 ggcaaggaag gccaga
//
LOCUS       DAOQLS010000135         3656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13137_length_3656_cov_3.638989, whole genome shotgun sequence.
ACCESSION   DAOQLS010000135 DAOQLS010000000
VERSION     DAOQLS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13137_length_3656_cov_3.638989"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PK362_03515"
     CDS             complement(<1..143)
                     /locus_tag="PK362_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73943.1"
                     /translation="MSFPTDETEARVLLSLHPKIGPVRFARLLERFASASAALAAPRE
                     AWES"
     gene            complement(210..599)
                     /locus_tag="PK362_03520"
     CDS             complement(210..599)
                     /locus_tag="PK362_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC73944.1"
                     /translation="MTYRNLLHLIFFIALGFWGWDRAMSHDGLIHFIDRHPDAWQADR
                     ALFFVGSFHELFNRDQKALEIYQRVLENYPESPAAEGAQYGAASCLERLRRWREAIVE
                     YQKYLEKYPQGRFSNSVNNNLQILRSR"
     gene            complement(613..1614)
                     /locus_tag="PK362_03525"
     CDS             complement(613..1614)
                     /locus_tag="PK362_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC73945.1"
                     /translation="MIRVAKLFVFLAAALSFAGVRAVAEETGAPAEAPAAEASAPTDA
                     TAPADGTTVTPADGAAEAPVEPATVDAAAPEAPSDGTYKIVKGDTLWNIANTYLKDPF
                     LWTKIWEANKSNITNPDLIYPDQQLVIPSVSAAQGMAETPAAPPTAETAAAPAVETPA
                     TTVEPVTEAPAATETAEALPAPAEKPKPIASAPEPEPEPEPVEEAPAVPVKKGSVPGA
                     GFMGGVSDTFVAGPDWEYDGYILRDREQKMMISQGDVVFLNVGAASGVQPRMTGYIYR
                     LGKKIRDPFLKKYTGKMLKRVGTVMVTSQVNDEGCTAVITNSLEPIRVGDLVKFAAN"
     gene            complement(1730..1945)
                     /locus_tag="PK362_03530"
     CDS             complement(1730..1945)
                     /locus_tag="PK362_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73946.1"
                     /translation="MARLFRPAVRAADAPPVFVLEKPPLPRDLAPVAARLERWREEGR
                     LSREEFERLAGLIREDAAAGGTTPRSP"
     gene            complement(1987..>3656)
                     /gene="glyS"
                     /locus_tag="PK362_03535"
     CDS             complement(1987..>3656)
                     /gene="glyS"
                     /locus_tag="PK362_03535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNC73947.1"
                     /translation="GRTAFARPIRWLVALYGNRVVPVTVAGVKSGKKTRGLRFHARKP
                     FDVPSPAKYADLLRDHCVIVDPAERRELIVKQIRHAVKTVHGHVPVERFGDLLTEVAG
                     LVEHPVAVVGSFDEKHLALPVEVLVTSMKKHQKYFPVYKDVAATALLPLFVGIRNGLS
                     DHQSVVREGYERVLAARLADAAFFYGQDRKRPLADRVPDLAGIAFLSPRLHLKDKTDR
                     VTVLVDLIVDRLGREAELGGEARRIAALSKADLTTGMVGEFPELQGVMGRIYAAADGE
                     APEVSAGVEEQYWPLTAEGPLPVLETAAIVSVADKIDTLAGNFLIGKIPSGSQDPYGL
                     RRAAVGVLRLLEERRWPLSLTDLIREAVRGLPDGLGDKPAAEKTLAEFFRQRWAALAE
                     ARGERFDEVDAVTAVGFHEVADARRRLEALRAVRQHPDFEPLSAAFKRATNLLKQAEK
                     KGEAPGDAPLQAETLVLPAEKALLESLRTVSEGVSAAQGRGDYAEAFGRLVALRAPVD
                     AFFTDIIVMDPNPELRRNRLQLLSSVRACFDRLADLSRLQDVRAPAK"
BASE COUNT          654 a         1184 c         1183 g          635 t
ORIGIN      
        1 gactcccagg cctcccgcgg agcggccaag gcggcggagg cggacgcaaa tcgctcaagc
       61 aatcgggcga agcgcaccgg cccgattttg ggatggagac tcaggaggac ccgggcctcg
      121 gtttcgtccg ttggaaagga cacgttcccc ccgggcccaa aaaaaacccg tgccgccggt
      181 tctcgggcgg gcgggcggac cccatcaaat tatctggatc ttaagatttg aagattgttg
      241 ttgaccgaat tggaaaaacg accctgcgga tacttttcca aatatttttg atattccaca
      301 atcgcttcgc gccaacgccg caaccgctcc aagcacgacg cggcgccgta ttgggcgcct
      361 tcggcggcgg gcgactccgg gtagttctcc aacacgcgct ggtaaatttc caacgccttc
      421 tggtcccgat tgaaaagctc gtgaaaactg ccgacgaaga aaagggcccg gtccgcctgc
      481 caggcgtcgg gatgccggtc gatgaaatgg atcaacccgt cgtgggacat ggcgcggtcc
      541 catccccaaa accccagggc gatgaaaaag atgagatgaa ggagattccg gtaggtcatc
      601 cccgggccgc gatcaattcg ccgcgaactt caccaggtcg ccgacccgaa tcggctcgag
      661 gctgttcgtg atcacggcgg tgcacccctc gtcgttgact tggctcgtca ccatcacggt
      721 gccgacccgt ttcaacattt tcccggtgta cttcttcaaa aagggatccc ggattttttt
      781 gccgagtcgg taaatgtagc cggtcatgcg gggttggacg ccggaggcgg ccccgacgtt
      841 caggaaaacc acgtcgccct gggaaatcat catcttctgt tcgcggtcgc gcaaaatgta
      901 gccgtcgtat tcccaatccg gacccgcgac gaaggtgtcg gagacgccgc ccatgaaccc
      961 ggcgccgggc acgctgcctt ttttcaccgg cacggcgggg gcttcctcca ccggttcggg
     1021 ttcgggctcc ggttccgggg cggacgcgat gggttttggt ttttcggccg gcgcgggcag
     1081 ggcttccgcc gtttccgtcg cggcgggggc ttccgtcacc ggctcgaccg tggtggccgg
     1141 cgtttccacg gcgggggcgg cggcggtttc ggccgtcggg ggcgccgccg gcgtttcggc
     1201 catcccctgc gcggccgaca ccgacgggat gaccagctgt tggtcggggt aaatgagatc
     1261 ggggttggta atgttggatt tgttcgcttc ccaaatcttc gtccaaagga agggatcttt
     1321 caaataggtg ttggcgatgt tccagagggt gtcgcccttg acgattttgt aggtcccgtc
     1381 ggacggcgcc tcgggcgccg ccgcgtcgac ggtcgccggt tcaacggggg cctccgccgc
     1441 gccgtcggcg ggggtgacgg tggtcccgtc cgcgggggcc gtcgcgtcgg tcggggcgga
     1501 ggcttcggcc gcgggcgctt ccgcgggcgc tccggtctcc tcggccaccg ctcggacccc
     1561 ggcaaacgac aaggcggccg cgaggaaaac gaaaagtttg gcgacacgaa tcataaagcc
     1621 tcctgaaggc aggatcatcg tctgggaacg ttctcggcgg gccagaagaa aggcaacatt
     1681 cctcgtaatt tgttcaacgg tttctttata atccaaaaag ggggcactgt caagggcttc
     1741 ggggggtcgt cccccccgcg gccgcgtcct cgcgaatcag gcccgccaaa cgctcaaatt
     1801 cttcccggga aagcctcccc tcctctcgcc aacgctccaa gcgggccgcc acgggggcca
     1861 aatcccgggg caaggggggt ttttcaagga cgaacacggg gggggcgtcg gccgcccgga
     1921 cggcgggacg aaaaaggcgc gccatccacc acaataaaac ggcccccccc accatccccg
     1981 cccaaatcat ttggcgggag cgcgcacgtc ctgaagacgg gaaagatcgg ccaagcggtc
     2041 gaaacaggcg cggaccgacg acaacaattg gagccggttt cggcggagtt ccgggttggg
     2101 gtccatgact ataatgtccg tgaaaaaagc gtccaccggg gcgcggagag ccaccaggcg
     2161 accaaaggcc tcggcgtaat ccccccgccc ctgggccgcc gacaccccct ccgaaacggt
     2221 ccgaaggctc tccaacaggg ctttttcggc gggcaacacc aacgtctccg cttgaagcgg
     2281 ggcgtcgccg ggggcttccc cttttttctc cgcttgtttc aataaattgg tcgcgcgttt
     2341 aaacgccgcc gacaacggct cgaaatccgg gtgttggcga accgcccgca gggcctccaa
     2401 tcgccgacgg gcgtcggcca cctcgtggaa acccacggcc gtcacggcgt cgacctcgtc
     2461 gaaccgctcg ccgcgcgcct cggcgagggc cgcccagcgc tgacggaaaa attccgccag
     2521 ggttttttcc gcggcgggct tgtcgcccag gccgtcggga agtccgcgca cggcttcgcg
     2581 aatcaagtcg gtcagggaga gcggccaccg tcgttcctcc agaaggcgga gcacgcccac
     2641 cgccgcccgg cgcaggccgt agggatcctg ggaaccggag gggattttgc cgatcaggaa
     2701 attgccggcc aaggtatcga tcttgtccgc gaccgagacg atcgccgccg tttccaaaac
     2761 cggcaacggg ccttcggccg tcaggggcca atattgttcc tcgaccccgg cgctgacctc
     2821 cggcgcctcg ccgtcggccg cggcgtaaat ccgccccata acgccttgga gttcggggaa
     2881 ctcgcccacc atgccggtgg tcaaatcggc cttggacagc gcggcgattc ggcgcgcctc
     2941 gcccccgagt tcggcctccc ggcccaaccg atcaacgatc aaatccacga ggaccgtgac
     3001 ccggtcggtt ttgtctttca ggtggagcct gggcgacagg aaggcgatgc ccgccagatc
     3061 cgggacgcgg tccgccaggg gccgcttgcg gtcctgaccg tagaagaacg cggcgtcggc
     3121 cagacgcgcc gccagcacgc gctcgtagcc ttcccgcacg acggattggt ggtccgacaa
     3181 cccgttgcga attccgacga agagggggag gagcgccgtg gcggcgacgt ctttgtatac
     3241 ggggaaatat ttctgatgct ttttcatcga agtgaccagc acctcgacgg gcagggcgag
     3301 gtgtttttcg tcgaaggagc ccacgacggc caccgggtgc tccaccaaac cggccacttc
     3361 ggtcaacagg tccccgaagc gctccaccgg cacgtgcccg tggacggttt tgacggcgtg
     3421 gcggatctgt ttgacgatca actcccggcg ctccgccggg tcgacgatga cgcagtggtc
     3481 ccgcaggaga tccgcgtatt tggccggcga ggggacgtcg aagggtttgc gcgcgtggaa
     3541 ccgcagcccc cgggttttct tcccggactt caccccggcc acggtcacgg gaacaacgcg
     3601 gtttccgtac aaagccacca accaccggat cggccgggcg aaggccgtcc ggccgg
//
LOCUS       DAOQLS010000136         3653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13156_length_3653_cov_6.306837, whole genome shotgun sequence.
ACCESSION   DAOQLS010000136 DAOQLS010000000
VERSION     DAOQLS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13156_length_3653_cov_6.306837"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PK362_03540"
     CDS             complement(<1..589)
                     /locus_tag="PK362_03540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC73948.1"
                     /translation="MAWSPKKKRVGALGALLMLGFLARVAWVVHDRAAAPHPDLMSFH
                     PSQLPFTHPFDTSPREPFYVWWLWILGAVGADSTAAIRLLGTLWFLPNAFLLFQLVRR
                     HWDDRTAWGALGLFAVLPGQIVSDGLGLRHLMETAGVLLFLTALRTESSLESRRTWLQ
                     AAGAFAALVLTRVTYAVPGGIFLTAAAFRKIPPGTA"
     gene            588..1886
                     /locus_tag="PK362_03545"
     CDS             588..1886
                     /locus_tag="PK362_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNC73949.1"
                     /translation="MRTERNIFDFHRIGRLGAAALLAAVGCLRNPITQKREAKLISDR
                     AEREIGLETKKKIIEEYGELKDPVFNQYVSNLGRRLASLSDRPKLDYQFTVLDTDVVN
                     AFAAPGGFIFVTRGLLEQVSNEAELASVLAHEIGHVAGWHSIGMIQKQMGLGTLTALG
                     AIASGIRIGPEAMIMVAQTADLFAALYLLGYSRENELEADRVGLRYMSSAGYDAGAAL
                     AFFKKLEALEKKDGPDRWESYLRSHPPTGERIAQAQAFLDRWTFFRRPRERGEGAYLE
                     MKARLPRLAAEEQGRVEGAKFDQPLYGVALTVPDGWSWEPQSGRAIAAFRRRDGEAWG
                     ELRREALKDPLTAEAYARKFVADRKGQWLQGREVLYPAGYGYLAQFYGPGLLGGTYQF
                     RGFFTVRDGAGWALLCAAVPDRALEHFVAFEQIQRSFELK"
     gene            complement(2128..2868)
                     /locus_tag="PK362_03550"
     CDS             complement(2128..2868)
                     /locus_tag="PK362_03550"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNC73950.1"
                     /translation="MSHPTHPPAAAEPLNIDEVGAPRGGAPQRAQTRLYLLLQVFSGC
                     RDTGPLVDALKNSGLESVLYLDAHDPSGVGLLFFAEDPGLFTADVRRLLATGPFAELT
                     QRHDMTMMGRTYSIGREVDLLDWLLKKPRRAARNAQWPWAVWYPMRRKATFEHLPREE
                     QGQIMLDHARIGMRFGDAGFVQDIRLACHGIDRNDNDFVLGLIGKELFPLSRIVQEMR
                     KTQQTALHMESLGPFFVGRALWQSPFSE"
     gene            3223..3295
                     /locus_tag="PK362_03555"
     tRNA            3223..3295
                     /locus_tag="PK362_03555"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3256..3258,aa:Phe,seq:gaa)
     gene            3419..>3653
                     /locus_tag="PK362_03560"
     CDS             3419..>3653
                     /locus_tag="PK362_03560"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HNC73951.1"
                     /translation="MWKWTADAVALLHVAWIAAFMVGPLWGLRRPGVRAAHLALMWIT
                     AGMWHYYCPLTVIENMIRVQYDPAGAYGGGFLNH"
BASE COUNT          707 a         1060 c         1242 g          644 t
ORIGIN      
        1 acgccgtccc cggcggaatc ttcctgaagg ccgcggccgt caggaagatt ccgccgggga
       61 cggcgtaggt gacccgggtg agaaccaggg cggcgaaggc cccggccgct tggagccacg
      121 tccggcggga ttcaaggctc gactccgttc gaagggcggt caggaacaaa aggacgccgg
      181 cggtttccat caagtgccgg aggcccaggc cgtcggaaac gatttgcccc ggcaaaacag
      241 caaagaggcc cagggcgccc caggccgttc gatcgtccca gtggcgccga accaactgaa
      301 aaagaagaaa agcgttgggg agaaaccaca acgttcccag gaggcggatg gccgccgtgg
      361 agtccgcccc caccgccccg agaatccaca gccaccagac gtagaacggc tcccggggcg
      421 aggtgtcgaa ggggtgcgtg aacggcagtt ggctcgggtg gaaggacatc aagtccgggt
      481 ggggcgccgc ggctcggtcg tgaaccaccc aggcgacccg ggccaggaag cccaacatca
      541 aaagggctcc cagagccccg acgcgttttt ttttcggaga ccatgccatg cggacagagc
      601 gtaacatttt cgattttcat cgaatcggac ggttgggcgc cgccgcgctc ctggccgcgg
      661 tcggttgtct ccgcaatccc atcacccaga agcgcgaagc caagctgatt tcggaccggg
      721 ccgaacggga aatcggcctc gagaccaaga aaaagatcat cgaggaatac ggcgagctga
      781 aagaccccgt tttcaatcaa tacgtctcca acctcggccg ccgattggcc tccctgagcg
      841 accgccccaa actggattat caattcaccg tcctggacac cgacgtggtg aacgccttcg
      901 ccgcgccggg cggctttatt ttcgtcaccc gggggctctt ggagcaggtg tccaacgaag
      961 ccgaactcgc ctcggtgttg gcccacgaaa tcggccacgt ggcgggttgg cattccatcg
     1021 gcatgatcca aaaacagatg ggcctgggga ccttgaccgc cctgggggcc atcgcctcgg
     1081 ggattcggat cggcccggag gccatgatca tggtggccca aacggcggat ttgttcgccg
     1141 cactctacct gctgggttac agccgggaaa acgaactgga ggccgaccgc gtgggccttc
     1201 gctacatgag ctccgccggc tacgacgccg gcgccgccct cgcgtttttt aaaaagctgg
     1261 aggccctgga aaaaaaagac gggcccgacc gctgggaatc ctacctgcgt tcccatccgc
     1321 cgaccggcga acgcatcgcg caagcccagg cgttcctcga tcggtggaca tttttccgcc
     1381 ggccgcggga aaggggcgag ggcgcgtacc tggaaatgaa agcgcgattg ccgcggctgg
     1441 ccgccgagga acagggccgg gtggaagggg ccaaattcga tcaacccctg tacggcgtgg
     1501 ccttgaccgt ccccgacggg tggtcctggg agccccaaag cggccgggcg atcgcggctt
     1561 tccggcggag ggacggcgaa gcttggggcg agctgcgccg ggaagctttg aaggacccct
     1621 tgaccgccga ggcctacgcc cgaaaatttg tggcggatcg gaaaggccaa tggctgcagg
     1681 ggcgggaggt cctttatccg gcgggttacg gctacctggc ccaattttac gggccgggcc
     1741 tgttgggggg gacgtaccag tttcgaggat ttttcacggt gcgcgacggg gccggctggg
     1801 ccttgctctg cgcggcggtg ccggaccggg ccctggaaca cttcgtcgcc tttgaacaga
     1861 tccagcgatc gttcgagttg aagtaaatcg gctccaatcc ccttcccccc gagggacgtt
     1921 cgaaaattga cccgtttgtt cgccggtcgt cgggcgggcg tcctccacgg gcgaaggacg
     1981 gagagcgtgt tccgagcgat ttgatctagg gccgggcggg gggaaaaggg gaaggcgact
     2041 ttgtgaggaa cgggcgtaaa aaccgtccga tccacggagc aaaaatcggc ccccgcgatc
     2101 gaaccctcga acgagagtga cgaagtttta ttcggaaaaa gggctttgcc agagggcgcg
     2161 gcccacgaag aaggggccca gggattccat gtgcagggcc gtttgctggg tttttcgcat
     2221 ttcttgaacg atgcgggaaa ggggaaagag ctctttcccg atcagaccca aaacaaaatc
     2281 attgtcgttc cggtcgatgc cgtggcaggc caggcgaatg tcctggacga atccggcgtc
     2341 gccgaagcgc atgccgatcc gggcgtggtc cagcatgatc tggccctgct cttcccgagg
     2401 caggtgttcg aaggtggctt tccggcgcat tggataccag acggcccagg gccattgggc
     2461 gttgcgggcc gcccggcggg gtttctttaa gagccagtcc aacaaatcca cttcccggcc
     2521 gatggaatag gtgcggccca tcatagtcat gtcgtggcgc tgggtcagtt cggcaaaagg
     2581 gccggtggcg agaagccggc gaacgtcggc ggtgaacaag ccggggtctt cggcgaaaaa
     2641 caagaggccc acgccgctgg gatcgtgggc gtcgagatag aggaccgact ccaggcccga
     2701 gtttttcaga gcgtccacca aagggcccgt gtcccgacag ccggaaaaaa cctggaggag
     2761 caaatagagc cgggtttgcg cccgttgggg cgcgccgccc cggggggcgc cgacttcgtc
     2821 gatgttcagg ggttccgcgg cggcgggggg atgcgttgga tggctcatgc gtccattcta
     2881 gcaattgggc gaaggaattt cttgggggcg gcgcgggttt tggtataatg ccgacttcga
     2941 agttctttgt ggaacgggac agatcggccg cggcctttag gccgatgtcg atttgctctg
     3001 caaaagggcg gcgccgccgc ttcaacctat ttttcgccgc atttgcccgg agggcaaata
     3061 cggcggggcg agcccggaac tattttggat gccgccctgt gccagaggca caggacaggc
     3121 aggcgggccc gggttcttgg aaattgaatt tttaaatttg atggccgccc tgtgccagag
     3181 gcacaggacg gcgcggcagc cgcagagcgg ccgcaacccc gcgccgaggt agctcagtcg
     3241 gtagagcaca ggtctgaaaa acctggtgtc gtcagttcga ttctgaccct cggcacatct
     3301 tgaaacacga aacgccgcgt ccctttgggg cgcggcgttt ttattgggcg gcctggaatg
     3361 tggggaagtc ccgtgatata gtggaaaccg cccgggtggc cgggccgagg agcctaaaat
     3421 gtggaaatgg acggcggacg ccgtcgcgtt gcttcatgtg gcgtggattg ccgcctttat
     3481 ggtggggccc ctgtggggcc tgcgccgtcc gggggtccgg gcggcccact tggctttgat
     3541 gtggatcacg gccgggatgt ggcattacta ttgcccgctc acggtgatcg aaaacatgat
     3601 tcgcgtccaa tacgacccgg ccggggctta cggcggcggt tttttaaacc act
//
LOCUS       DAOQLS010000137         3648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13189_length_3648_cov_3.960200, whole genome shotgun sequence.
ACCESSION   DAOQLS010000137 DAOQLS010000000
VERSION     DAOQLS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13189_length_3648_cov_3.960200"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="obgE"
                     /locus_tag="PK362_03565"
     CDS             complement(<1..1080)
                     /gene="obgE"
                     /locus_tag="PK362_03565"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNC73952.1"
                     /translation="MNFVDRATLHLLGGKGGDGCLSFRREKYIEMGGPDGATGGKGGD
                     VWLLADPQKNTLYDFTYRPQYRAEDGVNGRGRDQTGAEGEDLIIRVPPGTLVFRDGRL
                     LADLKVAGDKFLAARGGRGGRGNASFKTAANTAPHLSEKGEPGQDVKVDLELKLLADV
                     GLVGFPNAGKSTFLSVVTQARPKIANYPFTTLTPNLGVCAVHGATFVLADIPGLIEGA
                     HTGKGLGDEFLRHVERTRVLIHLVDLSGYEDKTPLKTFRALNEELASYSPKLAEKPQI
                     VAATKMDLTDSDKALAAFRKGLRGVPVFPISSATGKGIKELLSAVQKTLAEAPEAPVF
                     QPERLDVVLEPDYRAEKTADHRFRVW"
     gene            complement(1160..1444)
                     /gene="rpmA"
                     /locus_tag="PK362_03570"
     CDS             complement(1160..1444)
                     /gene="rpmA"
                     /locus_tag="PK362_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012306120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNC73953.1"
                     /translation="MASVKSQGSTSNGRDSHGQRLGIKAFGGEKVTAGSVIVRQRGTP
                     IAAGTNTAVGKDDTIFATADGVVKFEFRRGTLGRSGGRRRVSVYPVAANA"
     gene            complement(1446..1778)
                     /gene="rplU"
                     /locus_tag="PK362_03575"
     CDS             complement(1446..1778)
                     /gene="rplU"
                     /locus_tag="PK362_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNC73954.1"
                     /translation="MTEQTTYAVIRTGGKQYRVTTGEKLKIQKLPEAAGDAVTFSEVL
                     LVKDGDALKTGRPTVAGAAVKGQVLRQFRDKKVLVFKKRRKKGYKKLQGHRQDLTEVL
                     IESIQTGN"
     gene            1920..2570
                     /locus_tag="PK362_03580"
     CDS             1920..2570
                     /locus_tag="PK362_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73955.1"
                     /translation="MRSRKLLFSLFLLAAAGRGPLRAEELTATEQDLVSRYMQMHKGR
                     RDLAVGIWFPEAIWDRLASAAAEGSPGAALSAEDVLLLQKFLRPYTLVGLIYARQKGG
                     EVRPRPEAWVRKKTRLVGPDGRSYKALEPDRLPPELDQALRGFRFGLASTQPGLADYT
                     YFLAFPNVDKEGRLLVDPRREGSFSIKIGDANLLWTTPPDGEPRPTLPAGARPQPL"
     gene            2567..3196
                     /locus_tag="PK362_03585"
     CDS             2567..3196
                     /locus_tag="PK362_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HNC73956.1"
                     /translation="MTTATAVSLVYEYDGGLYVNLTSRCPTACAFCIKFSWDYKYRGH
                     DLKLPTEPTVDEILSAAPPDLSKYREVVFCGYGESTYRLAEMKTLAAAFRARGARRIR
                     LNTVGLGNLINGRNIAPELTFLDAVSISLNTVDPDTYLKIMRPRPEFRDRALASVKEF
                     IVECARAVPNTTVTAVALPGVDPAGVENVARAAGARYRLRPHLDAYEEE"
     gene            3132..>3648
                     /locus_tag="PK362_03590"
     CDS             3132..>3648
                     /locus_tag="PK362_03590"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HNC73957.1"
                     /translation="MPGRPAPDTVFAPTWTPTKKNRFSHRSATIRGPRADRPGPGRLG
                     VIADDFTGAGDAALALADRGWTVEFHARPGDRPGPRVRAWVINTDSRHDAPARAAAKV
                     RSAVRSLRRWGAVRLYKKIDSTLRGPLGAETDAFLRAGKFAGPLFFIPAFPGAGRTLV
                     GGRLLVNGVPLN"
BASE COUNT          610 a         1167 c         1158 g          713 t
ORIGIN      
        1 ccagacgcgg aagcggtggt cggccgtttt ttcggcgcgg taatccggtt ccaacaccac
       61 gtccaagcgc tccggttgaa agacgggcgc ttcgggggct tccgccaggg tcttctgcac
      121 ggcggaaaga agttctttga tcccttttcc cgtggccgaa gaaatgggaa aaaccgggac
      181 gcccctaagg ccttttcgaa aagccgccag ggccttgtcg gaatccgtca ggtccatctt
      241 cgtggcggcg acgatctggg gtttctccgc cagtttgggg gaataggagg ccagctcctc
      301 gtttaaggcg cgaaaagttt tgaggggcgt tttgtcctcg tagcccgaga gatccaccag
      361 gtggatcagc acccgggtgc gctccacgtg acgcaaaaac tcgtcgccca gccccttgcc
      421 ggtgtgggcc ccttcgatca ggccggggat gtcggccagc acgaaggtcg ccccgtggac
      481 ggcgcaaacg cccaggttcg gcgtcaacgt ggtgaacggg tagttggcga ttttggggcg
      541 ggcctgggtg accaccgaga gaaaggtgct tttgccggcg ttcgggaaac cgacgaggcc
      601 cacgtcggcc aggagcttca gctcgagatc gacttttacg tcctggcccg gttcgccctt
      661 ttccgacagg tggggggccg tgttggcggc ggttttaaag ctggcgttgc cgcgcccgcc
      721 ccggccgccc cgggcggcca aaaatttgtc cccggcgact ttcaggtccg ccagcagtcg
      781 tccgtcccgg aacaccaggg tgccgggagg gacccgaatg atcaaatcct cgccctcggc
      841 gccggtttgg tcccggcccc ggccgttgac gccgtcttcg gcccgatact gggggcggta
      901 ggtgaaatcg tagagggtgt ttttctgggg atcggccaaa agccaaacgt ccccgccctt
      961 gccgccggtg gcgccgtcgg ggccgcccat ttcgatgtac ttctcccgcc ggaaagagag
     1021 gcagccgtcg ccccccttgc cgcccagaag atgaagggtg gcgcggtcaa caaagttcat
     1081 gggaaaagtc ccttaaaaaa agatcagccc gagaggcggg ggcccctcgg gctgtggaga
     1141 tggatgggcc ggggcgctat taagcgttgg ccgcgacggg atacaccgac acgcgccggc
     1201 gaccgccgga acgaccgagg gtgccccgtc gaaattcaaa cttcacgacg ccgtccgcgg
     1261 tggcgaaaat ggtgtcgtct ttgcccacgg cggtgttggt tccggcggcg atgggcgtgc
     1321 cccgctggcg gacgatgacg gaaccggccg tcactttttc gccgccgaag gccttgatgc
     1381 cgagtcgttg gccgtgggag tcgcggccgt tggaggtgga gccttgggat tttacggatg
     1441 ccatgtcagt tccccgtttg aatcgattcg atcaacactt cggtcaggtc ctgccggtgc
     1501 ccctgaagct ttttgtagcc ttttttccgg cgtttcttga aaacgagaac cttcttgtcc
     1561 cgaaattggc gcaacacttg gcctttgacg gccgcgccgg ccacggtggg acggccggtt
     1621 ttgagcgcgt cgccatcttt gacgagaagc acttcggaaa aggtcacggc gtcgccggcc
     1681 gcttcgggca gcttttggat tttgagcttt tcgcccgtgg tcacacggta ttgtttgccg
     1741 cccgtgcgga taacggcgta ggtcgtttgt tcggtcataa ttcgcctatt ctagcaattt
     1801 aaaggaattt gtcaaagggg gagtacacaa aaaagaaccc gccccgccaa cggtgaacct
     1861 tcggcgggcc tgggagaagc gctccgtata ccaaagacat aagtgatata ctatttacga
     1921 tgcgatcgcg caagctcctg ttttcgttgt tcttgttggc cgcggccggg cggggccccc
     1981 ttcgcgccga ggaattgacc gccacggagc aggatctcgt ctcccgctac atgcaaatgc
     2041 acaagggccg gcgggatttg gccgtgggca tttggttccc ggaggccatt tgggaccgcc
     2101 tggcctccgc cgccgccgaa ggctcccccg gcgccgccct ctcggccgag gacgtccttc
     2161 ttcttcagaa attccttcgc ccctacaccc tggtcggttt gatctacgcc cggcagaaag
     2221 ggggcgaggt ccgcccccgg cccgaggcgt gggtgcgcaa aaagacccgt ttggtgggcc
     2281 cggacgggcg atcctataaa gccctggaac cggaccgcct tcccccggag ttggaccagg
     2341 cccttcgggg gtttcggttc ggcctggcct ccacccagcc cgggttggcc gattacactt
     2401 attttctggc tttcccgaac gtggacaagg aaggccgcct gttggtcgat cctcgccggg
     2461 aagggtcctt ttccatcaag atcggcgacg ccaaccttct gtggaccacg ccaccggacg
     2521 gggaaccccg gccgaccctg cccgccggcg cccgtccaca acccctatga ccaccgccac
     2581 cgccgtgtcc ctggtttatg aatacgacgg gggcttgtac gtcaatctga ccagccgctg
     2641 tcccacggcc tgcgcttttt gcatcaaatt ttcctgggac tataaatacc ggggccacga
     2701 tttgaaattg ccgacggagc cgacggtgga cgagatttta agcgcggccc cgcccgacct
     2761 ttccaaatac cgcgaagtgg tgttttgcgg gtacggggaa tccacctatc ggttggcgga
     2821 aatgaaaacc ctcgccgcgg ctttccgcgc ccggggagcc cggcgaatcc gtctcaacac
     2881 cgtggggctc ggcaacttga tcaacgggcg aaacatcgcc ccggaactga cctttttgga
     2941 cgccgtgtcc atcagtctca ataccgtcga cccggacact tatctgaaga tcatgcgccc
     3001 ccgcccggaa ttccgggacc gggccctggc cagcgtaaag gaatttatcg tcgaatgcgc
     3061 ccgggccgtt ccgaacacga ccgtaaccgc cgtggcgctt cccggcgtgg accccgccgg
     3121 cgtggaaaac gttgcccggg cggccggcgc ccgataccgt cttcgccccc acctggacgc
     3181 ctacgaagaa gaatagattc tcccatcgat cggcgacgat ccgcggcccc cgggcggatc
     3241 gcccgggccc cggacgtctc ggcgtcatcg ccgatgattt caccggcgcg ggcgacgccg
     3301 ccttggccct ggccgaccgg gggtggacgg tggagttcca cgcccgcccc ggcgatcgtc
     3361 ccggtccccg cgttcgggcc tgggtgatca acaccgattc ccgccacgac gcccccgccc
     3421 gggcggcggc caaggtgcgt tccgcggtgc gatcgctccg ccgatggggc gccgtccgtc
     3481 tctacaaaaa aatcgattcc acgctccggg ggcccctggg ggccgagacc gacgcttttc
     3541 tccgggccgg aaaattcgcg ggccctcttt ttttcatccc cgcttttccc ggggcgggtc
     3601 gaacgttggt gggcggccgg ttgctggtga acggggtccc tttgaacc
//
LOCUS       DAOQLS010000138         3627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13319_length_3627_cov_4.948488, whole genome shotgun sequence.
ACCESSION   DAOQLS010000138 DAOQLS010000000
VERSION     DAOQLS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13319_length_3627_cov_4.948488"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="PK362_03595"
     CDS             <1..1447
                     /locus_tag="PK362_03595"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC73958.1"
                     /translation="FRSRSGLVALSVGAGLLFHRRWGVRGTVWFVVALAAAVVLAPTS
                     RTAVVFKTIDPFGWSRLAIWKAAGAGILDRPLFGWGPGLFARLYGVHRGPFPHQWIRF
                     DHDHSFAHNEYLQLGAEYGIPAVLLAAAAFGPRIFGARRAADPARWAIFSATAVFCFF
                     NFPFYSPVNLLLAAGVFFGASPEPNSFSPSDRRERGLWRGTFAVLGVIALASFFAARP
                     TGTRFVFTKAQWENRLHRADALVHPAAGGEADPDEAEAILRETLRRWPKDPEAGHALG
                     HLLIEHRTPPRLEEGLPWLLRAAALAPTRAPWWMEVSVVQAALGRWPDALESAQNALR
                     LEPEYADAQLMIARALTRTGDPGEAERFLAPLRALPPQDPGSLSGYGQTITHRDRDAY
                     LLESAYAQLRLKNPAAALAFLSDRSAPPSAESLALSVSAHLARGDRRAARRLWNDLQA
                     SFPVSPAVKALAGAFESMNGKAPKGKIQPTP"
     gene            1444..2004
                     /gene="rfbC"
                     /locus_tag="PK362_03600"
     CDS             1444..2004
                     /gene="rfbC"
                     /locus_tag="PK362_03600"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNC73959.1"
                     /translation="MIVTTPTPLEGLLILEPRVHRDPRGYFFEAYNERDFRETGLPTD
                     FVQDNVSVSVKGTLRGLHFQRAPHAQGKLVRVLTGSVFDVAVDMRPSSPTFGKWFGLE
                     LSEENGRALYLPPGFAHGFLATAERVVFHYKVTSLYQPGTEGTLRWDDSTVGVQWPDQ
                     TGPILLSEKDRAGLSWKDALRLLSPK"
     gene            complement(2030..3181)
                     /locus_tag="PK362_03605"
     CDS             complement(2030..3181)
                     /locus_tag="PK362_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC73960.1"
                     /translation="MKRWWARAALLGIGALIGLAVSEIFARVYFRHLTEDFVVRSEDL
                     YYYNDAHGVRHHVPGKVGFEKSWDGVGRVAVRINSHGLRGPEISLPKSAGVRRLLILG
                     DSVTLSGHTREEDTFVSLLQEKLLRDGATGVEVLNAGVGNVGLREEKDFLESLLSLDP
                     SVVVLVWYLNDARPPLGFPEENVYANPIIRFVNRSECLHRSVLVRLLYEKTKNWLIRK
                     GIEGSRSLQSGYEWVTAFNEGRWRSSPSEFRRMVQLARFDWGDAWNESHLNWSAREIL
                     ELNQRLRKRKMRFGVVLAPVWPQVAIRTSDPFLQFPQRTLAAVCQRAGIPVLDLAEAL
                     RPRASADMFFDQCHFTPTGNRAVAECLARFLKEARMVSDGSETGGERFQ"
BASE COUNT          709 a         1164 c         1019 g          735 t
ORIGIN      
        1 gtttcgatcc cggagcggtc tcgtggcctt gtcggtcggg gccggcctcc tattccaccg
       61 ccgatggggc gtccggggaa ccgtgtggtt tgtcgtcgcc ttggcggcgg cggtggtcct
      121 ggccccgacc tcccgcacgg cggtcgtctt caaaaccatc gatcccttcg ggtggtcccg
      181 tctggcaatt tggaaagccg ccggggcggg gatcctggac cgtccgcttt tcggctgggg
      241 accggggctt tttgcccgcc tttacggcgt ccaccggggg ccgtttcccc accagtggat
      301 tcgcttcgac cacgaccatt ccttcgccca caacgaatac cttcaattgg gcgccgaata
      361 cggaattccg gccgtgcttc tcgctgccgc cgcgtttggt ccgagaatct tcggcgcccg
      421 acgggcggcg gatccggccc gttgggcgat tttttcagcg accgcggtat tttgcttttt
      481 caatttcccc ttttactccc ccgttaatct cctgctggcc gccggtgttt tcttcggggc
      541 gtctccggaa cccaattctt tttctccctc ggaccggcgc gagcgcgggc tttggcgcgg
      601 aaccttcgcc gtcctcggcg tcatcgccct cgcctctttt ttcgccgcgc gaccaaccgg
      661 gacccggttc gtctttacga aggcccaatg ggaaaatcgc ctccatcggg ccgacgcgtt
      721 ggtgcatcca gcggccggtg gggaggccga tcccgatgaa gcggaggcga ttcttcggga
      781 aaccctccga cggtggccca aggacccgga agcgggccac gccctcggcc atttattgat
      841 cgaacatcgg accccccccc ggttggaaga aggactccct tggctgctcc gggccgccgc
      901 tcttgcgccg acccgggccc cctggtggat ggaggtgtcc gtggttcagg ccgccctggg
      961 ccgctggccc gacgccctgg agtccgcgca aaacgccctt cggttggaac cggaatacgc
     1021 cgacgcccaa ttaatgatcg cccgggccct cacccggacc ggcgatcccg gggaagccga
     1081 acggttttta gcgcccctgc gggccctccc cccccaagac ccgggatccc tttccgggta
     1141 cgggcaaacc atcacccacc gggaccgcga cgcgtatttg ctcgaatcgg cctacgccca
     1201 actgcggctc aagaaccccg ccgcggcgtt ggctttcctg tccgatcgct cggccccccc
     1261 ttcggccgaa tccctggccc tgtccgtgag cgctcacctg gcccggggag accgccgcgc
     1321 cgcccgccgt ctctggaacg acctgcaagc gagttttccg gtcagcccag cggttaaagc
     1381 cttggcgggg gcctttgaat ccatgaacgg gaaggcccca aaaggtaaaa tacaacccac
     1441 cccatgatcg tcacgacccc cacgcctttg gaaggacttt tgattttgga gcctcgggtc
     1501 caccgggacc cccgggggta ttttttcgag gcgtacaacg agagggattt cagagagacg
     1561 gggctaccga ccgatttcgt tcaggacaat gtgagcgtat cggtcaaggg aaccttgcgg
     1621 gggcttcatt tccagcgggc gccccacgcc cagggcaagc tcgtccgagt gctgacgggc
     1681 tcggtgttcg atgtggccgt tgacatgcgg ccgtcgtccc cgacctttgg aaagtggttc
     1741 ggactggagc tgtcggagga aaacggccgg gccctttatc tccccccggg cttcgcccac
     1801 ggatttctgg ccaccgcgga acgcgttgtc tttcactaca aagtgacctc gctatatcaa
     1861 cccggcactg aagggacttt gcggtgggac gattcaaccg ttggagttca atggcccgac
     1921 caaacaggac ccatcctttt gtccgagaaa gaccgcgccg gattgtcctg gaaagacgcg
     1981 ttacggctcc tttccccgaa gtgaacccgg cgcaacggat gcaacggctc tattgaaatc
     2041 tctcgccccc ggtttcactc ccatctgaaa ccattcgggc ctctttcaga aaccgcgcga
     2101 gacattccgc caccgcccgg tttccggtgg gggtgaaatg gcactggtca aaaaacatat
     2161 ccgcactggc gcggggacgc aacgcctcgg ccaaatccag cacggggatt ccggcccttt
     2221 gacaaaccgc cgcgagggtc ctctgaggga actggaggaa cgggtccgac gtgcgtatcg
     2281 cgacctgggg ccacaccggc gccagaacaa ccccaaatcg catttttctt tttcttagtc
     2341 gttgatttaa ttcgaggatt tcacgggcgc tccaattcaa atgggattcg ttccaagcgt
     2401 ccccccaatc gaaccgagcc aattgaacca ttcgccgaaa ttcggagggc gacgaacgcc
     2461 agcggccctc gttgaacgcc gtgacccatt cgtagccgga ttgaaggctt cgggatcctt
     2521 cgatcccttt ccgaattagc caattttttg ttttttcata caacaaacga acgagcacgc
     2581 tccgatgaag acattcggag cgattaacga accgaatgat cggattggcg taaacgtttt
     2641 cctccggaaa acccaagggc ggacgggcgt cgttcaaata ccaaacaaga acaacgacgc
     2701 tcgggtctaa actcaataac gattcgagaa agtctttttc ttcccgtaaa ccgacgtttc
     2761 ccaccccggc gttaagcacc tccacccccg tcgccccatc gcgcaaaagc ttttcttgaa
     2821 gcaagctcac aaaggtgtcc tcttcccggg tgtggccgct aagggtcacg gaatcgccca
     2881 atatcaacaa ccgccggact ccggccgatt tgggaagaga gatttccggc cctcgaagac
     2941 cgtgcgaatt aatgcgaacg gcaacacgcc cgacgccgtc ccaagatttt tcaaatccca
     3001 cctttccggg aacgtggtgt cggaccccgt gcgcatcgtt gtaataataa agatcttcgc
     3061 ttcgaaccac aaaatcctcg gtcagatgcc gaaaatagac acgggcaaaa atttccgaca
     3121 ccgctaagcc gatcagcgca ccgattccca gaagggcggc gcgcgcccac caccgcttca
     3181 acggacctcc ttgaccacaa cgagattctc caccaccagg gcgtccatac cgctcgcctg
     3241 aaaagttctc catgcttctt gcggattgga cacaatgggc tcccctctca aattgaagga
     3301 tgtattgagg attacgggca cgccggtggc ggagccgaac cggtctatca atcggtgaaa
     3361 caagggactt accatcggcg aaacggtctg aagacgtccc gtcccatcga cgtgcgtgac
     3421 cgcgggaaga acggcttgtt tgtccgcccg aacgggcgcc accatttgca tgaaccgagg
     3481 ggggagatgg cgttcgggct cgggaaaatc aaaaaattcg acggcccgct cttccggaac
     3541 ggacggcgca aaggggcgaa aaggttcccg gaatttaatt tttgtgttga cgatttgaag
     3601 tgtgggagaa agattttaca aatctca
//
LOCUS       DAOQLS010000139         3617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13381_length_3617_cov_3.971364, whole genome shotgun sequence.
ACCESSION   DAOQLS010000139 DAOQLS010000000
VERSION     DAOQLS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13381_length_3617_cov_3.971364"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PK362_03610"
     CDS             <1..1510
                     /locus_tag="PK362_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNC73961.1"
                     /translation="PRPPYQLPPISLLSEPDNKGRQPAEDALVAKAKTLEQTLANFGV
                     EARTTDIHPGPVITRYDLEPAPGVKISSIVNLADDIALAMKATRVRVLAPIPGKGAVG
                     VEIPNSESVTVSLKEIIADGRFQNSVSPLTVALGKTSSGEPHVTDLAPMPHLLVAGAT
                     GAGKSVAIHTLIMSILLRAKPDQVKFCLIDPKRLELPVYDGLPHLYDPRTGPDAAQVI
                     TLPKEAAKALQRMVKVMEYRYEIFARANVRNIEGYNEKRRAQGLPPEFYIIVVIDELA
                     DLMLISAKEVEDCIQRLAQMARAVGIHLVLATQRPSVDVITGVIKANLPARIALRVAS
                     QIDSRVILDGPGAESLVGKGDMLFLPAGAPQPIRLQGAFVSEKEVEAVVNFCKSQGGP
                     DYVDIFAQIAAAESAQEDSETRQQLDEALRLVVERKRVSQDLLKAHFGSSARATNVLS
                     LLEVKGFIFKPEGTNKWEIYFDKIDDYFRIPGAPPPPADEPTTPTQSERASA"
     gene            1507..2157
                     /locus_tag="PK362_03615"
     CDS             1507..2157
                     /locus_tag="PK362_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNC73962.1"
                     /translation="MKRLLPVCLFLAAAASLRAQSADDVVARLQRAESEIQRLQFDYL
                     QVTRIPLTGESVKSRGAALFERPNRFRLDQKTPQPQTVVCDGKDLWFYLPARGQVLRD
                     SVDNWARSAGFPQGLSPFRLETAELRSKYDIALETPDAKTPVLRLTPRGDRAFPYDLR
                     VWVDLATGLPVKTELSSKSVTATTTVKNFKINVLLSPDAFRFSPPAGTDVIDAPLK"
     gene            2172..3017
                     /locus_tag="PK362_03620"
     CDS             2172..3017
                     /locus_tag="PK362_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNC73963.1"
                     /translation="MTDIAPGTIGQSLRNRRLQNNKTIEDVADATRIQAKFLKALEEE
                     RWAELPARVFLDGFLVKYAEFLGLDGQDLLRQLRQQLGQTDKPAFTNPEPVPEAAGGG
                     SVLQIRLPFVMLGIVLVILAGFYFYRSQQERPRSGPLNINSVGDTEPLPAVLTSTAAA
                     PTPAVVEHTATVRAQNLVWLRVWADNAVKYEGTLNAGETRRWTFQSLFRLRAGNLSRV
                     VVDVDGGAVPTSSSSNPGEIVWPGTNGERATLTLSAPAVRPTPRPGTAPAVLRSTAPA
                     SVSHE"
     gene            3014..>3617
                     /locus_tag="PK362_03625"
     CDS             3014..>3617
                     /locus_tag="PK362_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC73964.1"
                     /translation="MTRGRVAFIVLGCAKNQVEAESMSSRLAREGWEMTADLPNASVI
                     VVHTCGFLDSARKEAADTLADIRKAAPRAFLVMTGCFAQYLNGKSWPGVDALLGTGQF
                     HRLPDVIDEKRRLKKGLPAPAIPAADRSPSGYHDAGLPRPLRPGQQSAYVRLSEGCNH
                     RCTFCVIPQLRGNLKSRPAAEILAEGRGLVDRGIRELVLIS"
BASE COUNT          684 a         1280 c         1041 g          612 t
ORIGIN      
        1 cccgcgcccc ccgtaccaat tgccgccgat cagtctcctg agcgaacccg acaacaaggg
       61 ccgccaaccc gccgaagacg ccttggtggc caaagccaaa accctggaac agaccctggc
      121 caatttcggc gttgaagccc ggaccaccga catccacccg ggcccggtca tcacccgcta
      181 cgacctggaa ccggccccgg gcgtcaaaat cagttccatc gtcaatttgg ccgacgacat
      241 cgccctggcc atgaaggcca cccgggtgcg ggtgctggcc cccattcccg ggaagggcgc
      301 cgtgggggtg gaaattccca attccgaatc ggtgaccgtt tccctgaaag aaatcatcgc
      361 cgacggccgg tttcaaaaca gcgtctcgcc cctcacggtg gccctgggca aaaccagttc
      421 cggggaaccc cacgtcacgg atttggcccc catgccccac ctgttggtgg ccggggccac
      481 gggggcggga aaatccgtgg cgatccacac gctcatcatg tccatcctgc ttcgggccaa
      541 acccgatcaa gtcaaatttt gcctcatcga ccccaagcgg ctcgaactgc ccgtctacga
      601 tggattgccc catctctacg acccgcgcac gggccccgac gccgcccagg tcatcaccct
      661 tcccaaggaa gccgccaagg ccctccaacg catggtcaag gtcatggaat accgctacga
      721 aattttcgcc cgcgccaacg tccgcaacat cgagggctac aacgaaaaac ggcgggccca
      781 gggcctgccg ccggagttct acatcatcgt cgtcatcgac gagttggccg acctgatgct
      841 gatttccgcc aaggaagtgg aagattgcat ccagcgcctc gcccaaatgg cccgcgccgt
      901 gggcattcac ttggtcctcg ccacccaacg accctcggtc gacgtcatca cgggcgtcat
      961 taaggcgaac ttgccggccc gcatcgccct tcgggtggcg tcccaaatcg acagccgggt
     1021 cattttggac ggccccgggg cggaatccct ggtgggcaaa ggggacatgc tgttcctgcc
     1081 cgcgggcgcg ccccagccca tccgacttca aggggctttc gtttccgaga aggaagtcga
     1141 ggcggtggtt aatttctgca agagccaggg cggaccggac tacgtggaca ttttcgccca
     1201 aatcgccgcc gcggagtcgg cccaggagga ttcggaaacg cgccagcaac tggacgaagc
     1261 cctgcgcctc gtggtcgaac gcaagcgcgt ttcccaagac ctcctcaagg cccatttcgg
     1321 ctcctccgcc cgcgccacca acgtgctgtc cctgttggaa gtcaaagggt ttatttttaa
     1381 acccgaaggc accaacaagt gggaaatcta tttcgacaaa atcgacgatt atttccggat
     1441 cccgggcgcg cccccgccgc cggcggacga accgacgacc ccgacccagt cggaaagagc
     1501 gtcggcgtga aacgcctcct tccggtttgc cttttcctcg cggcggccgc ttcgctccgg
     1561 gcgcaatccg ccgacgacgt cgtggcccgc ctgcaacggg ccgaaagcga aattcagcgc
     1621 cttcaattcg attatctgca agtgacccgc attccgctca cgggcgagtc ggtcaaatcc
     1681 cggggcgcgg cccttttcga gcggcccaat cgctttcgcc tcgatcaaaa aaccccccag
     1741 ccccaaacgg tggtctgcga cgggaaggac ctgtggtttt acctgccggc ccggggccag
     1801 gtgcttcggg attccgtgga caattgggcc cgctcggcgg gcttccccca ggggctctcg
     1861 ccctttcggt tggaaacggc cgagctccgc tccaaatacg acatcgcctt ggaaacgccg
     1921 gacgcgaaga ccccggtcct ccgtttgacc ccccgggggg accgcgcgtt tccctacgac
     1981 ctgcgcgtct gggtggacct ggcgacgggg cttccggtca aaaccgaatt gtcgtccaag
     2041 agcgtcaccg ccacgaccac cgtcaaaaac tttaaaatca atgtcctttt gagccccgac
     2101 gcgtttcggt tttcgcctcc ggcgggaacc gacgtgatcg acgcgccatt gaaataaatc
     2161 gaaggaatcc catgaccgac atcgcccccg gcaccatcgg ccagtcgctg cgcaaccgac
     2221 ggctgcagaa caacaaaacc atcgaagacg tcgccgacgc gacccgcatc caagccaagt
     2281 tcttgaaagc cctcgaggag gagcgctggg cggaattgcc cgcccgggtg ttcctcgacg
     2341 gtttcctggt gaaatacgcc gaattcctcg gcctggacgg ccaggatctc cttcgccaat
     2401 tgcgccaaca actgggccag accgacaaac ccgccttcac caatcccgaa ccggtccccg
     2461 aggccgccgg cggcgggtcc gttctgcaaa ttcggttgcc ctttgtgatg ttgggcatcg
     2521 tgctcgtcat tctcgccgga ttctattttt accgttccca gcaggaacga ccgcgttcgg
     2581 gaccgttgaa catcaacagc gtcggcgaca ccgaacccct gccggccgtt ctgacgtcca
     2641 cggccgccgc gccgacgccc gcggtcgttg agcacacggc caccgtgcgc gcgcaaaacc
     2701 tcgtgtggct gcgggtctgg gccgacaacg cggtcaaata cgagggcacc ttgaacgccg
     2761 gcgaaacgcg gcggtggact ttccaatccc ttttccggct ccgggccgga aacctttccc
     2821 gcgtcgtggt ggacgtcgac ggcggcgcgg tgccgacctc gagcagctcc aaccccggcg
     2881 aaatcgtctg gcccggaacc aacggggagc gcgccacgtt gacgctctcg gccccggcgg
     2941 tccggcccac gccccgcccc ggaacggccc ccgccgtcct tcgctccacc gcgcccgcct
     3001 cggtctccca cgagtgacgc gcggccgcgt ggccttcatc gttcttggct gcgccaagaa
     3061 ccaggtggaa gccgaaagca tgtcgtcgcg tcttgcccgg gagggctggg aaatgacggc
     3121 ggaccttccc aacgcgtcgg tcatcgtggt tcacacctgc ggctttctgg acagcgcccg
     3181 gaaagaagcg gccgacaccc tggcggacat tcgaaaagcc gccccgcgcg cctttctggt
     3241 catgacgggc tgcttcgccc aatacctgaa cggaaaatcc tggcccggcg tggacgctct
     3301 cctgggcacg ggccaattcc accggcttcc ggacgtcatc gatgaaaaac gccggttaaa
     3361 aaaaggcctt cccgccccgg cgatacccgc ggccgaccga agccccagcg ggtaccacga
     3421 cgccggactt ccgcgccccc ttcgccccgg acaacaatcg gcctatgtgc ggctctccga
     3481 gggctgcaat catcgctgca ccttttgcgt cattccccaa ttgcgcggca atttaaaaag
     3541 ccggccggcc gccgagatat tggccgaggg ccggggactg gtggaccggg gcatccggga
     3601 gctggtgctg atcagcc
//
LOCUS       DAOQLS010000140         3608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13433_length_3608_cov_3.381086, whole genome shotgun sequence.
ACCESSION   DAOQLS010000140 DAOQLS010000000
VERSION     DAOQLS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13433_length_3608_cov_3.381086"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK362_03630"
     CDS             <1..372
                     /locus_tag="PK362_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC73965.1"
                     /translation="AVERRFAAGPPAIWGDAATLTEAFEGLVQNAWEAMPGGGRLLVE
                     TRWTPGAEFLIVRLSDNGRGITDEDRERAFQPYFTTKPGAGGLGLALARRVMEFHGGT
                     IRLDGAPGKGATVSLFFPLPR"
     gene            398..1780
                     /locus_tag="PK362_03635"
     CDS             398..1780
                     /locus_tag="PK362_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73966.1"
                     /translation="MNRPARTARVGRRALLAFLTLTLGAGRRPHRLTPREWTALKESG
                     AYHTGEGWAEIRGDRGAAREEAQSRALADLSRHLRVAVRASMIDRLGQSAGRTDERLE
                     SRVESYTELVLKQPLDRRDVFLHFPKSGIVTAVVALSKNRYAAAVDADLEAKRERLLA
                     LYRTGREAEDGGRPVDALRSYARARGEAHSALDGLPLILPGDILGEDLLAVLDRRIAA
                     LLGAWTLDCGDGPRVYDGDGAPVTLTITGRGAPGSPAPFRDFPLRAAWSHRPKYPVAR
                     VRTDGLGAARFSVPLDGFVETPRLRVEPDWEDLNMMPPPMGPACESVFHPRRRIRLAV
                     TAGSPEAAERLASELARTLGGLPWTVERGTVPFPGEAPGTTMSADFGLEIRAAETRQK
                     HPTADFYFVRLTGTARLYDFRENRWVFRESLPEERGDGASLETARGTAWTRWVPKVTA
                     RVRDRSAPLP"
     gene            complement(1785..2147)
                     /locus_tag="PK362_03640"
     CDS             complement(1785..2147)
                     /locus_tag="PK362_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73967.1"
                     /translation="MRFELASLEQCLQAQARLLRRLISFSKQEREALLRKSDAVAPNP
                     KLQRLHRQLSQAETLRRQAETRALGAGVGTADTRPDIALALFRCRRLHGHVELLSREL
                     GHLQVQISQAVRNARQAA"
     gene            2246..2953
                     /locus_tag="PK362_03645"
     CDS             2246..2953
                     /locus_tag="PK362_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNC73968.1"
                     /translation="MIRGIQANAQAMNALMLQQDVEAHNLANQNTTGFKRDRVRFELF
                     SQILRGQETLSPKPVVTTERTQGALTRTDNPLDLALQGSGWFSVETPEGVRYTRDGSF
                     KWDAAGRLVDAEGHPVQGTSGALKLDPEAPGAVMVGRDGTLTAGSTPLGRLKITHFAP
                     DAPLERGSGGRYAAGDTPAVAGASEVWQGYLESSTVNPVEGMVEMMSTLRLFEANQKA
                     FRVQDESLSRALQELAR"
     gene            2972..>3608
                     /gene="flgG"
                     /locus_tag="PK362_03650"
     CDS             2972..>3608
                     /gene="flgG"
                     /locus_tag="PK362_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNC73969.1"
                     /translation="MLRALWTAASGMSAQQLNVDTIANNLANVNTSGFKKLRVDYQDL
                     YYQTLKEPGAAEEGGVSPTGLEVGMGTYPVSTQKIFTPGDLENTENPLDLAIEGAGFF
                     QVSLPDGNKAYSRSGTFRLDAEGRVVTADGYLLEPALTVPADTTEITVSLDGTVSAKS
                     AASPVPAVIGNLELATFSNPPGLKSLGHSLYAETAASGAPLTGTPGAQGVGS"
BASE COUNT          607 a         1211 c         1202 g          588 t
ORIGIN      
        1 gccgtcgaac ggcgtttcgc cgccgggcca ccggcgattt ggggagacgc ggcaacgctg
       61 accgaggcct tcgaaggcct cgttcaaaac gcctgggaag ccatgcccgg gggcgggcgg
      121 cttctcgtcg aaacccgctg gacccccggc gcggaattcc taatcgtccg gctttcggac
      181 aacgggcggg gaataacgga cgaggaccgg gaacgggcct ttcaaccgta tttcaccacc
      241 aaacccgggg cggggggcct ggggctcgcc ctggcccgcc gcgtgatgga atttcacggc
      301 ggcaccattc ggctggacgg cgcccccggc aaaggcgcga cggtgtcgtt gttctttccc
      361 ctcccccgtt gaaagcggtt tccggacgag gctcttcgtg aatcggcccg cgcggacggc
      421 ccgcgtcgga cggcgcgccc tcctggcctt cctaacgttg accctcggcg ccgggcgtcg
      481 cccccatcgg ttgacccccc gggagtggac cgcgctcaaa gaaagcgggg cgtaccacac
      541 cggcgaaggt tgggcggaaa tccgcggcga ccgcggcgcc gcccgggagg aggcccaaag
      601 ccgggcgctc gcggatttgt cccgccacct tcgggtggcg gtgcgggctt cgatgatcga
      661 tcgcctgggg caatcggcgg gtcggaccga cgagcgtctg gaatcccgcg tggaatccta
      721 cacggaactc gttttgaaac aacccctgga ccggcgggat gttttcctcc atttccccaa
      781 aagcggcatt gtcaccgccg tcgtggccct ttccaaaaac cgttacgccg ccgcggtgga
      841 cgccgacctc gaggccaaaa gggagcgtct gttggccctc taccgtacgg gccgggaggc
      901 ggaggacggc ggccgtcccg tggacgccct gcggtcctac gcgcgggccc gcggcgaggc
      961 ccattccgcg ctggacggac tcccccttat ccttcccggg gatattctcg gggaagacct
     1021 tttggcggtc ctcgaccggc gaatcgccgc cctcctcggc gcctggacct tggattgcgg
     1081 agacggcccc cgggtttacg acggggacgg cgctccggtg acgctgacga tcaccggccg
     1141 cggcgcgccc ggctcgcccg cgcctttccg ggattttcct cttcgggccg cctggtccca
     1201 ccgaccgaaa taccccgtgg cccgggttcg cacggacggg ctgggcgcgg cccgattttc
     1261 cgtccccctg gacggattcg tcgaaacccc ccgccttcgg gtggaacccg attgggagga
     1321 tttgaatatg atgcctcctc cgatgggccc cgcctgcgaa tcggtgtttc acccccggcg
     1381 tcggatacga ttggcggtga cggccgggtc cccggaggcg gcggagcggc tcgcgtcgga
     1441 gctcgcccgg accctgggcg gccttccttg gaccgttgaa cggggcacgg tgccctttcc
     1501 cggcgaagcc ccgggaacga cgatgtcggc cgatttcggc ttggaaatcc gggccgcgga
     1561 gacgcggcag aagcacccca cggcggattt ttattttgtc cgcctgacgg ggaccgcccg
     1621 cctttatgat tttcgggaaa accgatgggt gttccgggag tcgcttcccg aggaacgggg
     1681 agacggcgcc tctttggaaa ccgcccgcgg gaccgcctgg acccggtggg tcccaaaggt
     1741 gacggcgcgg gttcgggacc gatccgcgcc cctgccgtga cccctcaggc ggcctggcgc
     1801 gcgtttcgaa cggcctggga aatttgaacc tgaaggtgcc ccagctcccg ggacaggagc
     1861 tccacgtgtc cgtgcagtcg gcggcaccgg aaaagggcca aagcgatgtc cggccgggtg
     1921 tccgcggtgc ccacccccgc ccccagggcg cgggtttcgg cctggcgccg gagcgtttcg
     1981 gcctggctca actgacggtg aaggcgttgg agcttcgggt tgggggcgac ggcgtccgac
     2041 ttccgaagaa gcgcctcgcg ctcctgcttc gaaaaggaga tcaaccggcg cagaagccgg
     2101 gcctgggcct gcaaacattg ctcaagggaa gccagttcga atcgcatggg gttatcctcc
     2161 gggaaaatta ttggccgggg cccgggaaac taaacggcga aggacttcgc acgctttttg
     2221 caactgggaa gacgaggagg aacccatgat tcgaggcatc caagccaacg cccaggccat
     2281 gaacgccctg atgcttcaac aggacgtgga ggcccacaat ctcgccaatc aaaacaccac
     2341 cgggtttaaa agggaccggg tgcgttttga acttttcagt caaattcttc gggggcagga
     2401 aaccctttcc cccaaaccgg tggtcaccac cgaacggacc cagggcgccc tgacccggac
     2461 ggataacccg ctggatctgg cccttcaggg atccgggtgg ttttcggtgg agacccccga
     2521 gggggttcgc tacacccggg acggatcctt taaatgggac gccgccggac gcctggtgga
     2581 cgccgagggc caccccgtgc agggaacctc cggcgcgttg aaattggacc cggaggcccc
     2641 gggcgcggtg atggtggggc gcgacggcac cttgacggcc ggatcgaccc ccctgggccg
     2701 cctgaagatc acccatttcg cccccgacgc gcccctggaa cgggggagcg ggggccgtta
     2761 cgccgcgggc gatacgcccg ccgtggccgg cgccagcgag gtgtggcagg ggtatttgga
     2821 gtcctccacg gtcaaccccg tcgagggcat ggtggaaatg atgagcacct tgcgtttgtt
     2881 cgaagccaac cagaaggcct tccgggtgca ggacgaatcc ctgagccggg cccttcagga
     2941 actggcgcga tagtttcgaa ggaggaatcg catgttgcgc gcactgtgga cggcggcctc
     3001 ggggatgtcg gctcaacaat tgaacgtgga caccatcgcc aacaatttgg cgaacgtgaa
     3061 cacctcggga ttcaagaaac tgcgggtgga ttaccaggac ctttattacc aaaccctcaa
     3121 ggaaccgggc gccgccgaag agggcggggt ctccccgacg gggctggagg tcggcatggg
     3181 aacctacccc gtgtccaccc agaaaatttt cacccccggg gacctggaga acaccgaaaa
     3241 tcccctggac ctcgccatcg agggcgccgg gttcttccaa gtctccctgc ccgacggcaa
     3301 caaagcctat tcccgttccg gcaccttccg cctggacgcc gaaggccgcg tggtcaccgc
     3361 cgacggttat ctgttggaac cggccctcac cgtgcccgcc gacacgacgg aaatcaccgt
     3421 ctccctggac ggcaccgtat cggccaaatc cgccgcgtcc ccggttccgg ccgtcatcgg
     3481 caacctggaa ttggccacgt tctccaatcc gccggggctc aaatccctcg gccacagcct
     3541 gtacgccgaa accgcggctt ccggcgcgcc tctgacgggg acccccggcg cccagggcgt
     3601 gggatccg
//
LOCUS       DAOQLS010000141         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13505_length_3595_cov_4.207910, whole genome shotgun sequence.
ACCESSION   DAOQLS010000141 DAOQLS010000000
VERSION     DAOQLS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13505_length_3595_cov_4.207910"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="PK362_03655"
     CDS             complement(<1..1907)
                     /locus_tag="PK362_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNC73970.1"
                     /translation="MTRRLFFIAALFLATALRAAESLPARGNADVTDNPLSVDFETFL
                     HDPLNAPLTDEVAGAVAQTGTKWARVMANWDFIERSTGVYTYQGLDNLMNRLKSHGFK
                     VFMTLNGGSHGGAGGGSPFYAGGNSPTSVNGALPPWLNFVDATVRRYRGDVSTWEIWN
                     EPNFDWQPSPNAADYAALAQVTIQKVRSLQPGATIILGGTSMVDKAFLTAVLPVVGSD
                     IDAVGLHPYRQYPEQSQDDFVALYPFLKPNAAFTPVGAASYTEEIAVVRAILSGLGLS
                     RVKIWSTEAGTASQNEASGLFPGTETKQAKNLCRYFLLNRSLGIERSSWFRALDQNLL
                     TNAYTPTGQAWVENYTAPSYMALGQFATFGPLALDPTTSLVIPTTATTGTLNISVSGS
                     TLTATNANNYAQYSVNVPTPGDYVFWFRLKGPDASTFGYLNAAVNGSSGTTSGSLTYI
                     YGAFSGLAAGLQGQSDLSKYYYSPLIYVGLDPAQELPYHPVFLPLSGTATLKVQINSG
                     SLSEIRLVRRGDLTRKKSFTALSSLGAVLDDRVGLAGSHRAAFANVNLSTSEWQSFQS
                     VSFLTQGKVPLVAYWIGTPPAEVFAPRSVRVSLPVLTDPVLIDPVEGPFQTLDSGGEF
                     PLPATDAPLILT"
     gene            complement(1910..2806)
                     /locus_tag="PK362_03660"
     CDS             complement(1910..2806)
                     /locus_tag="PK362_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73971.1"
                     /translation="MKKILCGALAGLVVFQPAAFAANDPRAGGTANFFRAAPAARALA
                     MGNAQTAVARGADAIFFNPAALAGTPRPEISTLFSAQEFDQTDGSLAAAAPFRRGVWG
                     LSAQFYSVGDILLRQREFDLSSRASGRSSAVTLAYGQTRGPIAVGVAVKYLSQSFSGL
                     SDRADGQALDLGAARKFDRLTLGVVLQNLLGQFRWSTGKKDDLRMILRAGAGYQANSW
                     LLAALDVEGGDGQSLRAHGGLEGRYKELAFRAGVNHDHPTLGFGLALPALGAVRPRLD
                     YALEFKAEGLNDAHAIGLSAQF"
     gene            complement(2845..>3595)
                     /locus_tag="PK362_03665"
     CDS             complement(2845..>3595)
                     /locus_tag="PK362_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNC73972.1"
                     /translation="PESLIESELFGHEKGAFTGADAARAGKFEQADGGTVFLDEIGNL
                     PLAVQVKLLRFLQNPSVERLGSRRGPVALNVRVVAATNVDLEAAVKNGTFREDLFHRL
                     KVFVVDMPPLRSRGTADLEGLLSVLLENFRRQLGKAKLSVSPESLQLFKAYPWPGNIR
                     ELQNALRSASLLADDVIRPDHLPVSVQNARPRPYSSGESGALNDVIRRVERDHIAKTL
                     EHCHGDRAAAARALGLDAATLEDKLKDFGLS"
BASE COUNT          678 a         1100 c         1220 g          597 t
ORIGIN      
        1 gtcaaaatca acggcgcgtc cgtggcgggc agggggaatt cgccgccgct gtccaacgtt
       61 tgaaaggggc cctccacggg gtcgatcaag accgggtccg tcaggaccgg cagggacacc
      121 cgcacgcttc ggggggcgaa gacctccgcc gggggcgttc cgatccaata ggccaccagg
      181 ggcaccttgc cctgggtgag gaaggagacg gactgaaagc tctgccattc ggaggtggac
      241 aaattgacgt tggcgaaagc ggcccgatgg cttcccgcca accccacccg atcgtccaag
      301 acggccccca aggaagacag ggccgtgaag gattttttac gcgtcaggtc tccccgacga
      361 accaggcgga tttcgctcaa agaccccgaa ttgatttgca ccttcaaggt cgccgtgccg
      421 gacaggggaa gaaaaaccgg gtggtagggc agttcttgag cggggtccag gccgacgtaa
      481 atcagcgggg agtaataata cttggacagg tcgctttggc cctggaggcc ggcggccagg
      541 ccgctgaaag cgccgtagat gtaagtcagg gaaccggacg tcgttcccga actaccgttc
      601 accgcggcgt tcaggtagcc gaaggtggag gcgtcgggac ccttgaggcg gaaccaaaaa
      661 acgtaatccc ccggggtcgg gacgttgacg gaatattggg cgtagttgtt ggcgttggtg
      721 gccgtcaagg tggagcccga cacgctgatg ttcaaggtgc ccgtggtggc cgtcgttgga
      781 atcaccaggg aggtcgtcgg gtccagcgcc agggggccga aggtggcgaa ttggcccaag
      841 gccatataag agggcgcggt gtagttttcc acccaggctt ggcccgtcgg ggtgtaggcg
      901 ttggtgagca agttctggtc cagggcccgg aaccaggagg agcgttcgat ccccagggag
      961 cggttcaaca gaaaataacg gcagaggttc ttggcctgct tcgtttccgt tcccggaaaa
     1021 agacccgagg cttcgttttg ggacgcggtg cccgcctcag tgctccaaat tttgacccgg
     1081 gacaatccga gaccggacag gatcgcccgg accacggcga tctcctcggt gtaggacgcc
     1141 gcccccaccg gcgtaaaggc ggcgttgggt ttaaggaagg gatagagagc gacgaaatcg
     1201 tcctgggact gctccgggta ttgccggtag gggtgaaggc ccacggcgtc gatgtcgctc
     1261 cccaccacgg ggagcacggc ggtcaagaac gccttgtcca ccatgctggt tccccccagg
     1321 atgatcgtgg ccccgggctg gagggacctt actttttgga tcgtgacctg ggccagggcc
     1381 gcgtaatccg ccgcgttggg cgagggttgc caatcgaagt tgggctcgtt ccaaatttcc
     1441 caggtggaca cgtcgccccg gtagcggcgc accgtggcgt ccacaaaatt cagccagggc
     1501 ggaagggcgc cgttgacgct cgtcggcgag tttcccccgg cgtaaaaggg gctccccccg
     1561 cccgcgccgc cgtggctgcc gccgttcaag gtcataaaaa ctttgaaccc gtgggacttc
     1621 aggcggttca tcaggttgtc caggccctgg taggtataga ctccggtgct ccgctcgatg
     1681 aagtcccagt tggccatgac ccgggcccac ttggttccgg tttgggccac ggcgcccgcc
     1741 acctcgtcgg tcaagggggc gttcaagggg tcgtgcaaaa aggtttcaaa atcgacggac
     1801 agggggttgt ccgtcacgtc ggcgttcccc cgggcgggaa ggctctccgc cgcccgaagg
     1861 gccgtggcca gaaacaaggc ggcgatgaaa aacagtcgtc gggtcatggt taaaattggg
     1921 cgctcaggcc gatggcgtgg gcgtcgttca gcccttcggc tttaaattcc agggcgtaat
     1981 ccaaacgcgg gcgaacggcg cccagggccg gaagggccag gccgaacccc agggtcgggt
     2041 gatcgtgatt gaccccggcc cgaaacgcca attccttgta gcggccctca aggcccccgt
     2101 gcgcccgaag ggattggccg tccccgcctt ccacgtccag ggccgccaac agccaggagt
     2161 tcgcttgata accggccccc gctcgcagga tcatccgaag atcgtctttt ttccccgtgg
     2221 accaacggaa ctgccccaaa agattctgaa ggacgacgcc cagggtcagc cggtcgaatt
     2281 ttcgcgcggc ccccaggtcc agggcctggc cgtcggcccg gtccgaaaga ccggagaagg
     2341 attgggacag gtatttgacc gcgaccccga cggcgatggg cccccgggtt tgtccgtagg
     2401 ccagggtcac cgccgaggac cgacccgagg cccggcttga aagatcaaat tcccgctggc
     2461 gcaacagaat gtcccccacc gaataaaact gcgcgctcag gccccacacg ccccggcgga
     2521 agggcgccgc ggccgccagg gaaccgtcgg tttggtcgaa ttcctgggcg gagaaaaggg
     2581 tcgaaatctc cggccggggg gtcccggcca gggccgccgg attaaaaaaa atcgcgtcgg
     2641 ccccccgggc cacggccgtt tgggcgttgc ccatggcgag ggcccgggcc gcgggcgccg
     2701 cccggaaaaa attggccgtt cccccggcac ggggatcgtt ggcggcgaag gccgccggtt
     2761 ggaaaacgac gagacccgcc agggccccgc aaagaatttt tttcatggaa gggaagaccg
     2821 tccgaaaacc ggcggcgcgc cggctcagga caagccgaaa tcctttaact tgtcctcgag
     2881 ggtggccgcg tccaggccca gggcccgggc ggccgcggcc cggtcgccgt gacagtgctc
     2941 cagggtcttg gcgatgtggt cccgctccac ccggcggatg acgtcgttta aggccccgct
     3001 ctccccggag gagtagggcc ggggccgggc gttttgaacg ctcacgggca aatggtcggg
     3061 ccgaatgacg tcgtcggcca acagagaggc gcttcgaagg gcgttttgca gttcccgaat
     3121 attgcccggc caggggtagg ccttgaacaa ttggagggat tcgggcgaca ccgacaactt
     3181 ggccttcccc aactgccggc ggaaattttc caaaaggacg gacagcaggc cctccagatc
     3241 ggccgtgccc cgggaccgaa ggggcggcat gtccacgacg aagactttca ggcggtgaaa
     3301 gaggtcctcc cggaaagtgc cgtttttgac ggcggcttcg agatcgacgt tggtggccgc
     3361 caccacccgc acgttcaagg ccacggggcc ccggcggctg cccaggcgtt ccaccgacgg
     3421 gttttgcaga aagcgcaaca gcttcacttg gacggccagg ggcaggttgc cgatctcgtc
     3481 cagaaacacc gtgccgccgt cggcctgctc gaacttcccg gcccgggccg cgtcggcgcc
     3541 cgtgaaggcg cctttttcgt ggccgaagag ttcgctttcg atcagggatt ccgga
//
LOCUS       DAOQLS010000142         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13519_length_3594_cov_3.289065, whole genome shotgun sequence.
ACCESSION   DAOQLS010000142 DAOQLS010000000
VERSION     DAOQLS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13519_length_3594_cov_3.289065"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK362_03670"
     CDS             <1..717
                     /locus_tag="PK362_03670"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC73973.1"
                     /translation="ATIPTAVPTKTPATPAAEVASWDWLSDPIPAEKKLQRLRELIGD
                     CRRCPLGAPRIQLAFGVGNPEARVLFIGEGPGYAEDRKGEPFVGPAGQLLDKILAATE
                     LSRQAVTPAWKWVYMVKCHPMIDPTDNTKRGNDRPPTPEEMAVCSPFLMAQIRIVRPL
                     FIVALGATAGKALLRTEEGISKFRGRWFDYSPDGDPALKIRLLPTYHPAALLRNPALK
                     KDVWEDMKNLKAELEKAAAR"
     gene            719..2716
                     /gene="priA"
                     /locus_tag="PK362_03675"
     CDS             719..2716
                     /gene="priA"
                     /locus_tag="PK362_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNC73974.1"
                     /translation="MAEPFLAEVVFPLPLRRAFDYRVPPTLDAAIRPGHRVWAPFGAR
                     GRKMGIVVRRHPAEGAVRSLDSLKTIDAPVESDVALPERETALARWMADRYWCSLGEA
                     AFTVSALGRKLPPKRPVPPRTPEARERPAPFDPTPDQQGAIDVVVPAVRAGGFRPFLI
                     HGVAAAGKTEVYRRAIEAALEGGRGAILLVPEIGLTPQMEDLFRRWFGAALEVWHSHI
                     ADGDRWRIWRRARSGECRLVIGPRSALFVPVTPLGLIVVDEEHDASYKQDSSPHYHAR
                     DVAWEKARLHGATLLLGSATPSLETLQAAREGRVESLALPRRVDDRPFPAVRLIDTRK
                     AGWYISDELVNAIRDRLAKKEQSLLFLNRRGYATRLRCRACEWESRCPRCAVSLVLHR
                     DPPADALRCHLCEHSEPPPDRCPQCRGEMIKATGRGTQKVLKDLATLFPAARLLRWDR
                     DAMSRRGSQTRAYESVRAGDVDIVVGTQMVAQGHDFPRLTLVGVLDADSALRLPDFRA
                     AERTFQLITQVAGRAGRADRPGEVIVQTRHPDHYALRSVQSRNPASFVEEELSFRREA
                     GYPPFARLAMLRVRAVRADRAEAAAEALTRTLESPDLPDGAAILGPAPAFHERREDES
                     QWQVLLKATPALFPEILQRLEKFEAPTGVSLSVNVDPEDLD"
     gene            2716..>3594
                     /locus_tag="PK362_03680"
     CDS             2716..>3594
                     /locus_tag="PK362_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73975.1"
                     /translation="MAALLLFLSAFAFFVSHLRPSIGPGEGGRWAALSAVLDPRTGPD
                     HALTALLGHAATILFPFGSPAFRLNALAALLVAASAPLLFGFLRGASDGDAPPSSLEA
                     LDRSRARDAGFFTAALLWLFSGPVRTAAASWGPGAPAIFLVFVFLWSLGVRPAGSRAA
                     AACLRGFLGGLALAADPRHAWGLLALLPFAWTHRPRDRRSVGAFLWALPAAFVGWGLA
                     DGIWAALQSATAREALVLFWKALPDRAAAFLPAVGWLRPALPSIAPAPLPPTAWPLAL
                     WGAQCLWRRNRPLLESL"
BASE COUNT          496 a         1318 c         1147 g          633 t
ORIGIN      
        1 gcgacgatcc cgacggccgt cccgacgaag accccggcca ccccggcggc cgaggtggcg
       61 tcttgggatt ggctgtccga cccgatcccg gcggaaaaaa aactgcaaag actgcgggag
      121 ttgatcgggg attgccggcg atgccccctg ggcgcgccgc gcattcaatt ggcttttggc
      181 gtgggaaacc ccgaggcccg ggtgctcttc atcggcgaag gccccggcta cgcggaggac
      241 cggaagggcg agcccttcgt gggcccggcg gggcaattgc tggacaaaat tttggccgcc
      301 accgaactct cccgccaggc ggtgacgccc gcttggaaat gggtttacat ggtcaagtgc
      361 caccccatga tcgaccccac ggacaacacg aagcgcggca acgaccgccc gccgacgccg
      421 gaggaaatgg ccgtctgctc cccctttctc atggcccaga tccgcattgt ccggcccctg
      481 ttcatcgtcg ccctgggggc cacggcgggc aaggcccttc ttcggaccga ggaggggatt
      541 tcgaaattcc gcggccgctg gttcgattat tcgcccgacg gggaccccgc gctcaagatc
      601 cgcctgttgc cgacctacca ccccgccgcg ctcctccgga accccgccct taaaaaggac
      661 gtttgggagg acatgaaaaa cctcaaggcc gagctggaaa aagccgccgc tcggtaatat
      721 ggccgaaccg tttttggcgg aggtcgtttt tcccctgccg cttcggcggg ccttcgatta
      781 ccgggtgccc ccgaccctcg acgcggcgat tcgccccggc caccgggtgt gggcgccttt
      841 cggcgcccgg gggcgaaaaa tgggcatcgt cgtgcgccga catcccgccg agggcgccgt
      901 ccgatcgttg gactccctca aaaccatcga cgcccccgtc gaaagcgacg tggccctccc
      961 cgagcgggag accgccctgg cccgctggat ggccgaccgc tattggtgct ccctcggcga
     1021 agccgccttc accgtttcgg ccctgggccg gaaactcccc cccaaacgcc ccgttccgcc
     1081 ccggaccccg gaggcccggg agcgccccgc cccctttgac cccaccccgg accaacaagg
     1141 cgccatcgac gtcgtggttc ccgccgtgcg ggccgggggc tttcgcccct ttttgatcca
     1201 cggcgtggcg gccgcgggaa aaacggaagt ttaccgtcgg gccattgaag cggccctgga
     1261 aggcggacgg ggagcgatcc tgctcgtccc cgaaatcggc ttgacgcccc agatggagga
     1321 tttgttccgc cggtggttcg gcgcggccct cgaggtgtgg cacagccaca tcgccgatgg
     1381 cgaccgatgg cggatctggc gccgggcccg gtccggcgaa tgccggctgg tgatcggccc
     1441 ccgttcggcg ctcttcgttc ccgtcacccc cctggggctg atcgtggtgg acgaggaaca
     1501 cgacgcctcc tacaaacaag acagctcccc ccattaccac gcccgagacg tcgcttggga
     1561 aaaggcccgt ttgcacgggg cgaccctcct gttgggaagc gccacgccgt ccttggagac
     1621 gctccaggcc gcccgggaag gccgcgtgga atccttggcc ctgccccggc gggtcgacga
     1681 ccggcctttt cccgccgtcc gcctcatcga cacgcgtaag gcggggtggt atatttccga
     1741 tgaactcgtg aacgcgatcc gggaccgcct ggccaaaaaa gaacagtccc tccttttcct
     1801 caaccggcgg ggctacgcca cccgtctgcg gtgccgggct tgcgaatggg aaagccggtg
     1861 cccccggtgc gcggtgagcc tcgtgctcca ccgggacccg cccgccgacg ccctccgttg
     1921 ccacttgtgc gaacattccg aaccgccgcc ggaccgctgt ccccagtgcc ggggcgaaat
     1981 gatcaaggcc accggtcggg gcacccaaaa agtgttgaag gatttggcca cgcttttccc
     2041 cgccgcgcgc ttgttaagat gggaccggga cgccatgtcc cgccgggggt cccaaacccg
     2101 ggcctacgaa tccgtccggg cgggggacgt ggacatcgtg gttggaaccc aaatggtggc
     2161 ccagggccac gactttcctc ggttgacctt ggtgggcgtc ttggacgccg acagcgcgct
     2221 ccggctgccg gatttccgcg ccgccgaacg gacctttcaa ttgatcaccc aggtggcggg
     2281 acgggcgggc cgcgccgatc gccccgggga agtgatcgtt caaacccggc accccgacca
     2341 ctacgccctg cggtcggtcc aatcccggaa cccggcgtct ttcgtggagg aggaattgag
     2401 ttttcgacgg gaagcgggct acccgccctt cgcgcggctc gccatgctcc gggtgcgcgc
     2461 cgtccgggcc gaccgggccg aagccgccgc cgaagccctg acccggacct tggaatcccc
     2521 cgacctcccc gacggagcgg cgattctggg acccgccccg gcttttcacg agcggcggga
     2581 ggatgaatcc cagtggcagg tgttgctcaa ggcgacgccg gcccttttcc cggaaattct
     2641 tcaacgcttg gaaaaattcg aagcgcccac gggcgtgagc ctctccgtca acgtggaccc
     2701 cgaggacttg gactgatggc cgcgctcctt cttttccttt cggccttcgc ctttttcgtg
     2761 tcccatctcc ggccgtccat cggaccgggg gaaggagggc gttgggcggc cctttcggcg
     2821 gtgctcgatc cccgcaccgg ccccgaccac gcgctcacgg cgcttctcgg ccacgccgcc
     2881 acgatcctct tccccttcgg ttcgcccgcc tttcgcctga acgccctggc cgccctgctg
     2941 gtggcggcct ccgcgcccct cttgttcggc tttttgcgcg gcgcttccga cggcgacgcg
     3001 ccgccttcgt ccctggaagc cctcgaccgc tcccgggccc gggacgcggg gttctttacg
     3061 gccgccctcc tctggctctt ttccggtccg gttcgaaccg ccgccgcttc ctggggcccg
     3121 ggcgcgcccg caatcttttt ggtgttcgtt ttcctctggt ccctgggcgt tcggcccgcc
     3181 gggtcccggg ccgcggccgc gtgtcttcgg gggtttttgg gcgggctggc tctggcggcc
     3241 gaccctcgcc acgcctgggg tcttctggcc ttgctgccct tcgcctggac ccaccggccc
     3301 cgggaccgtc gatcggttgg cgcctttctg tgggccctcc ccgcggcttt cgtcggttgg
     3361 ggcctggccg acgggatttg ggccgccctt caaagcgcca ccgcccggga agccctcgtc
     3421 cttttttgga aagccctgcc ggatcgggcg gccgccttcc ttcccgccgt cggatggcta
     3481 agaccggccc ttccatcgat cgccccggcg ccgctgcccc ccaccgcctg gcccctggcc
     3541 ctttgggggg cccaatgcct ttggcggcgg aatcgtccgc tactggaatc cctc
//
LOCUS       DAOQLS010000143         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13561_length_3588_cov_4.129635, whole genome shotgun sequence.
ACCESSION   DAOQLS010000143 DAOQLS010000000
VERSION     DAOQLS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13561_length_3588_cov_4.129635"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PK362_03685"
     CDS             <1..259
                     /locus_tag="PK362_03685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC73976.1"
                     /translation="VESRAVLGEKLGTGPLSFANPYGDAADDPAVQSLVREAGYRTAV
                     SVHQGKADLSGNPYCLRRLFIRGDDTAWDFHLNLTRGRARL"
     gene            267..1274
                     /locus_tag="PK362_03690"
     CDS             267..1274
                     /locus_tag="PK362_03690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNC73977.1"
                     /translation="MKILIVQPRRIGDVIVTTPVIDALRSRFPDARIEFLVEKGMAPV
                     LEGYPGLDEALVFEKSRFWFWLREIRRRRYDWVLDFMNNPRTAQLTWASRAPVRAGFE
                     TPFWGLVYTHRVARPPRPLYAVQIKFNLLRRLGLTPGERVLPRIPLSAADFAPAAGWW
                     ADRGLDAYPERIAVLPMHRRPIRQWPLARFAETVQRLLAVPDRAVLLFGGPEERRDLE
                     KMAGGFPRRVFAIPPGGLRQAAALLSRCHAAVTNDSGLMHLSVAVGLPTVTVYGPTWP
                     ESWNPRAAPHRYVQARGLTCLGCNRDQCPFGHECMDWVSVDRVVAEAEAVLAERRVGT
                     P"
     gene            1271..2344
                     /gene="lpxK"
                     /locus_tag="PK362_03695"
     CDS             1271..2344
                     /gene="lpxK"
                     /locus_tag="PK362_03695"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNC73978.1"
                     /translation="MNFPRPSSPLDIAWRAAWWGPARLFGWAAAARARAYRRGHFETV
                     RVPVPVVCVGNLTVGGSGKTPVVAWLAERWRAAGRRPGILSRGYGRTSRGLTVLANRP
                     GERLPPVEAVGDEPRWLAGRLPGVPLAIGADRARSAERLWEEFHPDVLILDDGLQHYR
                     LHRDADLICLDARLAHAVWVEGRPAPLLPAGPWREPPAALTRGRALLLTRAERLTPDE
                     REALRRRLSFFKGPIGAARYRIRLWEGERPVPDGELAGRPVLALSGLADPTSFEDALR
                     RLGAEVSPARFGDHHDFSAAEAAGAVDRARREGRLLVTTEKDAQRLPPGYPARRAELV
                     LEWEDTAWTTIVDSAIALNPSAS"
     gene            2293..3174
                     /locus_tag="PK362_03700"
     CDS             2293..3174
                     /locus_tag="PK362_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC73979.1"
                     /translation="MDDDRRFRYRLESLGVLGLVRVLGSLPPMLGRRAGAALGRILGA
                     VAGKRRRRAEENLRLAFPEVDDAQRAAWARDLWLQLGRAAWEFARLARLSPEAYLASV
                     DVRGLDRVQASAAAGKGVLLFTAHIGNWEYTTPFLSLAGLRLAVIARRIKNPYVDALV
                     TAVRARFNTRVIRHREAVRESLRWLRSGGVLGLLFDQRITEGGLSVPFFGRPARTTGL
                     PALLALRVGCPVHPVHSYREGDRLIIECGPALDISPAPPTEENLRAVMGQMTAVVEGW
                     IRRHPSQWLWIHNRWKP"
     gene            3215..>3588
                     /locus_tag="PK362_03705"
     CDS             3215..>3588
                     /locus_tag="PK362_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC73980.1"
                     /translation="MIERRLKIAVAARNAAGLSGASTLLWEESRRLAAQGHDVHVFAE
                     RVDEARLTAAGVRPRRLLRWPWGSRIKRRWFAGRVDRAVRRGGFDLVHGHGDLLNQDV
                     LSLHNCVHTAHERVRGAPLPAGS"
BASE COUNT          470 a         1226 c         1258 g          634 t
ORIGIN      
        1 ggtcgaaagt cgggcggttc tgggcgaaaa actgggaacg ggcccccttt ccttcgccaa
       61 cccctacggc gacgccgcgg acgaccccgc cgtgcagtcg ctcgtccgcg aggcgggata
      121 ccgaaccgcc gtgtcggttc atcaagggaa agccgacctg tccggcaatc cctactgcct
      181 ccgccggctt tttatccgcg gcgacgacac cgcctgggat ttccatttga acctcacccg
      241 cggccgcgcc cgcctttaac ccgcccgtga aaatcctcat cgtccagccc cgccggatcg
      301 gcgacgtcat cgtcaccacg ccggtcatcg acgcgctccg ttcccggttt ccggacgccc
      361 gaatcgaatt cctggttgaa aagggcatgg ccccggtgct ggagggctac ccgggtttgg
      421 acgaggcgct cgttttcgaa aagtcccgat tctggttttg gcttcgggaa atccgccggc
      481 gccgatacga ttgggtgttg gattttatga acaacccgcg gaccgcccaa ttgacctggg
      541 ccagccgggc gcccgtgcgg gccggttttg aaacgccctt ttggggcctg gtgtacaccc
      601 accgggtggc ccggcctccc cgcccgctct acgcggtgca aatcaaattc aacctcctcc
      661 gccggttggg cctcacgccg ggcgagcggg tgttgcctcg aattcccctg agcgccgcgg
      721 atttcgcgcc cgccgccggg tggtgggccg atagagggtt ggacgcctat ccggagcgga
      781 tcgccgtact tcccatgcac cggcgaccca ttcgtcaatg gcccctcgcg cgatttgccg
      841 aaaccgtcca gcgcctgctg gccgttcccg accgggcggt cctcctcttc ggcgggccgg
      901 aagaacggcg ggacctggag aaaatggccg gcgggtttcc ccgtcgcgtg tttgcgattc
      961 cgcccggcgg tttgcggcag gccgccgcgc ttctgtcccg gtgccacgcc gcggtcacca
     1021 acgattcggg gctgatgcat ctgtccgtgg ccgtcggcct tccgacggtc accgtctacg
     1081 gtcccacgtg gcccgagtcc tggaacccgc gcgcggcgcc gcaccgatac gtccaagccc
     1141 ggggactgac ctgcctgggc tgcaaccggg accaatgccc ctttgggcac gagtgcatgg
     1201 attgggtctc cgtggaccgg gtggtggccg aggcggaggc cgttctggcc gaacggcgcg
     1261 tcgggacccc gtgaatttcc cccggccatc gtcccccttg gacatcgcgt ggcgggccgc
     1321 gtggtggggc cccgcccggc tgtttggttg ggccgcggcc gcccgcgccc gggcctatcg
     1381 gcggggccat ttcgaaacgg tccgggtgcc agttcccgtc gtgtgcgtgg ggaatttaac
     1441 ggtgggcggg tcgggcaaaa cccccgtcgt ggcctggttg gcggaacgat ggcgggccgc
     1501 ggggcggcgg cccgggatcc tgtcccgggg gtacgggcga acgtcccggg gcctgaccgt
     1561 tctcgccaac cggccggggg agcgcctgcc gccggtcgag gcggtggggg acgagccccg
     1621 ttggctggcc gggcgtctgc ccggtgttcc cctggccatc ggcgcggacc gggcccgttc
     1681 cgcggaacgg ctctgggagg aatttcaccc cgacgtcctg atcctggacg acggtcttca
     1741 acattatcgg cttcaccggg acgcggattt gatttgtctg gacgcccggt tggcccacgc
     1801 ggtgtgggtg gaggggcgcc cggcccctct tctgccggcg ggtccctggc gggagccgcc
     1861 cgcggccctg acccgggggc gggcgctgct gttgacccgg gccgagcgtt tgacccccga
     1921 cgaacgggaa gccctccgcc gacgattgtc gttttttaaa ggccccatcg gggcggcccg
     1981 ttaccggatt cgcctgtggg agggcgagcg gccggtcccc gacggggaat tggcggggcg
     2041 gccggtgctg gccctgtcgg gcctggcgga tccgacgtcc tttgaagacg cccttcggcg
     2101 cctgggcgcc gaagtgtccc cggcgcggtt cggggatcat cacgattttt ccgccgcgga
     2161 ggccgccggg gcggtggacc gggcgcgccg ggagggacgc cttttagtga cgacggaaaa
     2221 agacgcgcag cgattgcccc cggggtatcc cgcccggcgg gcggagctgg tactcgaatg
     2281 ggaggacacg gcgtggacga cgatcgtcga ttccgctatc gccttgaatc cctcggcgtc
     2341 ctaggtctcg ttcgcgttct cggaagcctt ccgccgatgc tgggccgccg ggcgggagcg
     2401 gccctcgggc gaatcctggg cgccgtggcg ggaaagcgac gccgacgggc cgaggagaat
     2461 ttgcggttgg cctttcccga ggtggacgac gcgcagcgcg cggcctgggc ccgggatctt
     2521 tggctccagt tggggcgcgc cgcctgggag ttcgcgcgat tggcccgcct gtcgccggaa
     2581 gcctatctgg cgtcggtcga cgtccggggg ttggaccggg tccaggcctc ggcggccgcg
     2641 gggaagggcg ttctcctgtt caccgcccac atcggaaatt gggaatacac gacgcccttt
     2701 ttgtccctgg ccgggctgcg attggccgtc atcgcccggc gcatcaaaaa cccttacgtc
     2761 gacgccctgg tgaccgccgt ccgggcccgg ttcaacaccc gggtgattcg tcaccgggaa
     2821 gccgtccggg aatccctccg ctggctgcgg tccggcgggg tcctggggct cctgttcgac
     2881 cagcgcatca ccgagggcgg gttgtcggtc ccgttttttg gacgtccggc ccgaacgacg
     2941 ggcctgccgg ccctgctggc tctccgcgtc gggtgccccg tccatcccgt ccattcctac
     3001 cgcgaggggg accgattgat catcgaatgc ggtcccgctt tggatatttc ccccgcgccc
     3061 cccaccgagg aaaaccttcg cgccgtcatg ggccaaatga ccgccgtcgt cgaagggtgg
     3121 atccggcggc atccgtccca atggctttgg attcacaacc gttggaaacc gtgagccggc
     3181 ggtgggaacc ttccggtcgc gaatgatatg atccatgatt gaacgacggc taaaaatcgc
     3241 cgtggccgcc cgaaacgcgg ccggcctgtc cggggcctcc acgcttctct gggaagaatc
     3301 ccgccgcttg gccgcccagg gacacgacgt gcacgtgttc gccgagcggg tcgacgaggc
     3361 ccgcctgacc gcggccgggg tgcgcccccg gcggttgctc cgctggccct gggggagccg
     3421 aatcaagcgc cggtggttcg ccggacgcgt cgatcgggcc gttcgccgag gcggatttga
     3481 cttggttcac ggccacggcg acctgctgaa tcaagacgtg ctcagtctgc acaattgcgt
     3541 tcacaccgcc cacgagcggg tgcggggcgc tccgttgccc gccgggtc
//
LOCUS       DAOQLS010000144         3587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13569_length_3587_cov_4.278879, whole genome shotgun sequence.
ACCESSION   DAOQLS010000144 DAOQLS010000000
VERSION     DAOQLS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13569_length_3587_cov_4.278879"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PK362_03710"
     rRNA            <1..603
                     /locus_tag="PK362_03710"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            710..826
                     /gene="rrf"
                     /locus_tag="PK362_03715"
     rRNA            710..826
                     /gene="rrf"
                     /locus_tag="PK362_03715"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1101..1637
                     /gene="rplJ"
                     /locus_tag="PK362_03720"
     CDS             1101..1637
                     /gene="rplJ"
                     /locus_tag="PK362_03720"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNC73981.1"
                     /translation="MPSLRKEREESVQTLTQDFDGLEGLVVAGYVGVKTPELNELRGK
                     LRPIKAKCQIVKNRLAKIALKNKGIDGAFSDFFTGQSALVIQKGDAIAGLKVLVEFEK
                     AHENWKIRAGFINGKVIKPAEIKAMASLPSRNVLLAQLLGRLQGPLQGFHGVLNAHLQ
                     NLAGALDQIAKQKEKQSK"
     gene            1677..2063
                     /gene="rplL"
                     /locus_tag="PK362_03725"
     CDS             1677..2063
                     /gene="rplL"
                     /locus_tag="PK362_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNC73982.1"
                     /translation="MAKCTADDVLESIDAWTVLDVHKLVKGLEDKYGITAAPAAVAVA
                     AGGGAAGAAPAAEEKTSFSVVLMGAGAAKINVIKVVRELTGLGLKEAKDLVEAAPKPV
                     KEGLDKAAAEDMKKKLVEVGATVEVK"
     gene            2133..>3587
                     /gene="rpoB"
                     /locus_tag="PK362_03730"
     CDS             2133..>3587
                     /gene="rpoB"
                     /locus_tag="PK362_03730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNC73983.1"
                     /translation="MKKVSFARIHPAIELPQLLEMQTLSYEEFLQKDASPSERKIQGL
                     QAAFMDVFPITSTDEVLSLEFMHYTLGEPRYTVEEALAKDANYSSQLRAWLRLVQKQP
                     GGKPKVLTEQEVYFCDLPLMTTPATFVINGVERVVVSQLHRSPGVIFEEDEEKKISSY
                     GKKLYFARLIPYRGAWVEFEFDLNNAIFVRIDKKRKLPATTLLRAMGVETDAEILSLF
                     YDVEALNLASAQSEDVVGKIVAKDVVDTSSGEIVLEANKEITREVFLRLQDKKVRSVD
                     VLKSDPQVNDVAIRNTLLKDNIKTRKEAIQAIYRVVRAQEFIVPEQAESYLENMLFKS
                     IRKYDLTKVGRFKINRKLMPFLESLAERKDFKFEVPNDRRRTLCREDVIATLQYLILL
                     NNEATTHTFGKTTVKVEIDDIDHLGNRRIRSVGELMENQIRAAIAQMARLVRERMNVQ
                     ENVQITPRTLVNASAVVAAVRRFFGSSQLSQFMDQ"
BASE COUNT          817 a         1051 c         1027 g          692 t
ORIGIN      
        1 ctcctgcgta caagggtttc gttcggcacg actttggttt ctaacttacc gagccggtga
       61 gttaactata cgaccaaggt aacacgccta gctcccgtca gaataggacg ggatgatgat
      121 atagtctgca cccaatggaa attgggatta ttgtgtatcg gatccaagag cccatatcga
      181 cgatccggtt tggcacctcg atgtcggctc atcgcatcct cccgctggag tcggtgggaa
      241 gggtttggct gttcgccaat taaagcggta cgtgagctgg gttcagtacg tcgtgagaca
      301 gtacggtctc tatctaccgt aggcgcagga catttgaggg tgagtcgtct ctagtacgag
      361 aggaccgaga tgaacgcgcc tctggcgtac cggttggccc gcccgggcca tggccggata
      421 actattgcgc ggaacggata aacgctgaaa gcatctaagc gtgaagccag ccccaagatg
      481 agatgtcccg tccccttgtg ggacctaaag accaggagaa gactactccg ttgatcggcg
      541 ggatgtggaa gtgcggcaac gcacgtagct aacccgtacg aatcggtcgt gaggcttagt
      601 cgcatctgtg aatccaacat gagaagcgct tgactttcct atccgtgagt tgaggaccgc
      661 cggtccagct ctttttcaat ccaaacgcac gtcgtacggg ggcagaattc ccggtgcttg
      721 tcgcgatggg gtcacacccg ttcccattcc gaacacggcc gttaagccca tccgcgctga
      781 tggtacttgg cccgcaaggg cccgggagag taagtcagtg ccgggagttg tgctcctgac
      841 gcttgcgttt tggacgcact tttcatacaa tgggttgccc tttccaggcg atccatttcc
      901 atagccatgc ggcaatgacc gtaggggtcg caactcaccg cgactgaatt ggctcgagtg
      961 agcctcccta ccgaggcgcc cttttttgtt tgtcagaggg cggcccgacc tcatgaggcg
     1021 tacgaggacc gggacgcgtt gaagaaaaca ctcttcaccg tcggtgggag tgtttttttg
     1081 tttttacggg gaaaaatacg atgcccagct tacgcaaaga acgagaagaa tcggttcaaa
     1141 ccctcaccca ggactttgac ggcctggagg ggttggtcgt cgccggctac gtcggcgtca
     1201 agaccccgga gctcaacgag ctccgcggca aacttcgccc gatcaaggcg aaatgtcaaa
     1261 tcgtcaaaaa ccgcctggcg aagatcgccc tgaaaaacaa ggggatcgac ggggcgtttt
     1321 cggatttctt caccggccaa tcggccctgg tgattcaaaa aggcgacgcg atcgccggcc
     1381 tcaaggttct ggtggagttc gaaaaggccc acgagaactg gaagatccgc gcgggcttca
     1441 tcaacggcaa agtcatcaag cccgccgaga tcaaggccat ggcgtctctg ccgtcgcgca
     1501 acgttttgtt ggcgcagttg ttgggccgtt tgcagggccc ccttcaaggg ttccacggcg
     1561 tgctcaacgc gcatcttcaa aatctggccg gcgcgctcga ccagatcgcc aagcaaaaag
     1621 aaaagcagtc caaataatcg ttttcagggc aaacgccctc tcggaggagg aagaccatgg
     1681 caaagtgcac agcagacgac gtcctcgaat ccatcgacgc gtggaccgtt ttggacgttc
     1741 acaagctcgt caagggcttg gaagataaat acggaatcac cgcggctccc gcggcggtcg
     1801 cggtggcggc cggtggcgga gcggcgggcg cggccccggc ggccgaagag aagacgagtt
     1861 tctccgtcgt tctcatgggc gcgggcgcgg cgaagatcaa cgtcatcaaa gtcgttcggg
     1921 aactcacggg cctcggcttg aaagaagcca aggatctcgt cgaagccgcc ccgaagccgg
     1981 tcaaggaagg tttggacaag gccgccgccg aagacatgaa gaagaagttg gtggaagtcg
     2041 gcgccacggt tgaagtgaaa taagagaatt cggactcgaa tcaacggaat tcatttcccc
     2101 ttgatcgacc cccaaggtca gacaggactc gaatgaaaaa ggtctcgttc gcccgtattc
     2161 acccggccat tgaactgccc cagcttctgg agatgcagac gctgtcctat gaagaatttc
     2221 ttcagaagga cgcgtcgccc tccgagcgga aaatccaggg gctgcaagcg gcgttcatgg
     2281 acgtgttccc gatcaccagc acggacgaag tcctttcgct cgagttcatg cactacaccc
     2341 tgggcgagcc ccgctacacc gtcgaagagg ccctggcgaa agacgccaat tactcctcgc
     2401 agctgcgcgc gtggctccgt ttggtccaaa agcaaccggg cggcaaaccc aaagtcctga
     2461 ccgaacagga agtctacttc tgcgacctgc cgctcatgac cacgccggcc accttcgtga
     2521 tcaacggggt ggagcgcgtc gtggtcagtc agctgcaccg gtcccccggc gtgatcttcg
     2581 aagaggacga ggaaaagaag atctcctcct acggcaaaaa gctctacttc gcgcgcctca
     2641 tcccctaccg cggcgcctgg gtggagttcg agttcgacct gaacaacgcc attttcgtcc
     2701 gcatcgacaa gaagcgcaaa ctgccggcga cgaccttgct ccgcgccatg ggcgtggaga
     2761 cggacgccga aattttgtcg ctcttctacg acgtggaagc cttgaacctg gccagtgccc
     2821 aatccgagga cgtcgtcggc aagatcgtcg ccaaggacgt ggtggacacc agctccgggg
     2881 aaatcgtcct cgaagccaac aaggaaatca cgcgggaagt cttcctgcgt ttgcaggaca
     2941 agaaagtccg gtccgtggac gtcctcaagt cggatcccca agtcaacgac gtggccatcc
     3001 gcaacacctt gctcaaggac aacatcaaga cccgcaagga agccatccag gccatttacc
     3061 gcgtggtccg cgcccaggaa ttcatcgttc ccgagcaggc cgaatcctat ttggaaaaca
     3121 tgttgttcaa gtccatccgc aaatacgacc tgacgaaggt gggccggttc aaaatcaacc
     3181 gcaagctcat gcccttcctc gagtccctcg cggagcggaa agattttaaa ttcgaagtgc
     3241 cgaacgaccg tcgccggacc ctctgccggg aagacgtcat cgcgacgctc caatatttga
     3301 tccttctcaa caacgaagcg acgacccata cgtttggaaa aacgaccgtc aaggtggaga
     3361 tcgacgacat cgaccatttg ggcaaccgcc gcatccgttc cgtcggcgag ctcatggaaa
     3421 accagatccg cgccgccatc gcccagatgg cccgcctggt ccgggagcgc atgaacgttc
     3481 aagaaaacgt ccaaatcacg ccccgcaccc tggtgaacgc ctccgccgtc gttgcggccg
     3541 tccgccgttt cttcggctcc agccagcttt cgcagttcat ggaccag
//
LOCUS       DAOQLS010000145         3583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13601_length_3583_cov_3.598073, whole genome shotgun sequence.
ACCESSION   DAOQLS010000145 DAOQLS010000000
VERSION     DAOQLS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13601_length_3583_cov_3.598073"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>1309)
                     /locus_tag="PK362_03735"
     CDS             complement(66..>1309)
                     /locus_tag="PK362_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC73984.1"
                     /translation="PVGGAGPWLTGFREFSLSALRAVVANKVRSALSMLGILIGVAAV
                     IAMLALGRGAQKAVESRLSSLGSNLIMLMPGAPSSGGVRGEAGSATRLTIEDVKAIAK
                     VNPHVVRVDGNVNGSVQVVAGDQNTNTQLTGATPLYAPMRAAAPYYGRFFTDAEDDAE
                     ARVVLLGQTVINNLFGRENPVDKTIKINRVNFKVIGILPAKGAGGFRDQDDMVLVPLR
                     TAMNRVLGKKYLNSIAIECDSADGMPGVMEDVTALMRKRHRLPAHKDDDFALRNMADI
                     QQALAGTTQTFTLLLGIVAAISLLVGGIGIMNIMLVSVTERTREIGLRKAVGAAQWMV
                     LSQFLIESAVLSTLGGLIGIVLGGGVSFAMSRAAGWAAEVTWPSVALAFFFSVGVGIV
                     FGFWPARKASRLSPIEALRYE"
     assembly_gap    1310..1409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1538..2149)
                     /locus_tag="PK362_03740"
                     /pseudo
     CDS             complement(1538..2149)
                     /locus_tag="PK362_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2197..3186)
                     /locus_tag="PK362_03745"
     CDS             complement(2197..3186)
                     /locus_tag="PK362_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNC73985.1"
                     /translation="MKKNKVWIAAALLIVGAGLYFFTRRRAETPAEGTTQRVVQAHRG
                     PIELTVEATGVVSPLNRVEIKPPIAGRIEDIRVAEGSRVEKAQVLAWMSTTDRAAILD
                     AARAQGPEALKRWDDVYRPTPIAAPLAGEIILKNIVVGQTVDQSSVLFALSDRLIVLA
                     QVDEVDIGRVTEGLPARITLDAYPQLSIPGRVFNILHEGKNVSNVITYGVKVAPEKTP
                     PFFRSQMTANVSFVLQRKESALLVPAAAVQDRNGVKQVLVPGADGKPSAREVTTGLEN
                     GESVEILTGLNEGESVLVVRKKYTPQKGESSSPLVMSPGRRSGSASPRSGGAR"
     gene            complement(3183..>3583)
                     /locus_tag="PK362_03750"
     CDS             complement(3183..>3583)
                     /locus_tag="PK362_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC73986.1"
                     /translation="WTASGVLNWPIFGKGLTAAFFDAAAARRDWKAREEDLRAVRLEL
                     RRSLESAWSDWAARADRVAVQNQFLAAIEQQNSEASIRYASGLMSFENWEQVVTDLVN
                     VERSALQAERDAAVAEAAWRNARGVPLEDQ"
BASE COUNT          625 a         1150 c         1117 g          591 t
ORIGIN      
        1 cacggacact ctggggaata tttggagcac gtactaggaa gggaactccc gcgacttcga
       61 aaagcctatt cgtagcgcaa cgcttcgatg ggggagaggc gggaggcctt gcgggcgggc
      121 cagaagccga aaacgatgcc gacccccacg gaaaaaaaga acgcgagggc caccgagggc
      181 caggttactt cggcggccca cccggccgcc cgggacatcg cgaaggatac gccaccgccc
      241 aggacgattc cgatcaaccc gcccagggtg gagagcaccg ccgattcgat caagaattga
      301 gaaagcacca tccattgggc ggcccccacg gcctttcgca ggccgatttc ccgggtgcgc
      361 tcggtcaccg acaccagcat gatgttcatg atgccgatgc cgccgaccaa gagggaaatc
      421 gccgccacga tgccgagaag cagggtgaac gtctgggtgg tgccggccag cgcctgctga
      481 atgtcggcca tgtttcgaag ggcgaaatcg tcgtctttgt gggcgggcag gcggtggcgt
      541 ttgcgcatga gcgccgtgac gtcctccatc accccgggca tgccgtcggc cgaatcgcat
      601 tcgatggcga tggaattcag atatttcttg cccagaacgc ggttcatggc cgtccgcagg
      661 ggaaccaaaa ccatgtcgtc ctggtcccga aaaccgccgg cgcccttcgc cggaagaatc
      721 ccgatcacct tgaaattgac ccgattgatc ttgatcgtct tgtccaccgg gttttcccgc
      781 ccgaacagat tgttgatcac ggtctgcccc aaaaggacca cccgggcctc cgcgtcgtcc
      841 tcggcgtcgg tgaaaaaccg cccataatag ggagcggccg cgcgcatggg cgcgtacagc
      901 ggggtggcgc cggtcagctg ggtgttggtg ttttgatccc cggccacgac ctgcacagag
      961 ccgttgacgt tcccgtccac acgcaccacg tggggattga ccttggcgat ggccttcacg
     1021 tcctcgatgg tcaggcgggt ggcgctcccc gcttcgcccc ggaccccgcc cgaagacggg
     1081 gccccgggca tcagcatgat caaattggac cccaggctgg acaagcggga ttccacggct
     1141 ttctgggcgc cccggcccag ggcgagcatg gcgatgacgg ccgccacgcc gatcagaatt
     1201 cccagcatgg aaagggcgct ccgcactttg ttggccacga cggcccgaag ggccgacagc
     1261 gaaaattccc ggaaccccgt gagccaaggg ccggcgcccc cgacgggggn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acagcgaaaa ttcccggaac cccgtgagcc
     1441 aagggccggc gcccccgacg ggggaggtcg ccccttcgtc gaacggcccc ggaacgccga
     1501 ccggtcggtc ggcgccgggc gcccgctcgt ccccgacgat ccgcccgtct ttcaggcgga
     1561 gaatccgggt ggcgtgggcg gcgatttccg gttcgtgcgt caccatcacg atggaaatcc
     1621 ccgcggcgtg gaggcggtcc aattgggcca aaatctcctc cgcttgatcc gacgccagat
     1681 tgccggtggg ctcgtcggcg aaaatcaagc ggggccggtt ggccagggcc cgggcgatgg
     1741 ccacccgttg ttgctggccg ccggacaatt ggttgggccg atggtccagg cggtccccca
     1801 accccaccga cgtcaagagc tcccgggccc gcgccggccg cccaggcgcg ttggcgtaaa
     1861 tcatcggaag ttccacgttg gcctgggccg tggtgcgtgg gagcagatta aacatttgaa
     1921 agatgaaccc gatggtccgg gaacggaggg cggcgccttc gtcgtcggtc agacgggaga
     1981 cgtcccggcc caacagttta taggcgccgg aggtcgggcg gtccaggagg cccaaaatct
     2041 gcagcagcgt ggatttgccc gatcccgaag gccccatgat ggccacaaat tccccttccc
     2101 gaatggtcag ggtgacgttt ttcaggacct ccagttcttc gccgtccatg cggtagcatc
     2161 ggcgaatgtc ccgaagctcg atcagcgggg cggcggtcag cgggcccccc cggaccgggg
     2221 ggacgcggac cccgaccggc gtcccggcga catgacgagg ggactggagg actccccttt
     2281 ttgcggggtg tactttttac gaaccaccag gaccgactcg ccctcgttca acccggttaa
     2341 aatttcgacg ctctcgccgt tttccagccc ggtggtcact tcccgcgcgc tcggttttcc
     2401 atcggccccc gggaccagca cctgtttgac gccgttccga tcctgaaccg ccgccgccgg
     2461 aacgaggagc gccgattcct tgcgttgaag gacgaaactc acgttggccg tcatctggga
     2521 acggaaaaag ggcggcgttt tttccggcgc cactttcacg ccgtaggtga tcacgttgga
     2581 gacgtttttc ccctcgtgca aaatattgaa cacccggccg ggaatggaca actgggggta
     2641 ggcgtccagg gtgatgcggg cgggaagccc ctcggtcacc cgcccgatgt ccacctcgtc
     2701 cacctgggcc aaaacgatca aacggtccga gagggcgaac aggaccgagc tttgatccac
     2761 cgtctggcca acgacgatgt ttttcaaaat gatttccccc gccagcgggg ccgcgatggg
     2821 cgtgggccgg taaacatcgt cccaccgctt gagggcctcg gggccctggg cccgggcggc
     2881 gtccaaaatg gcggcccggt cggtggtgct catccaggcc aagacctggg ctttttccac
     2941 ccggcttcct tccgcgaccc ggatgtcttc gatgcgcccc gcgatgggcg gcttgatctc
     3001 cacgcggttc agcggcgaga cgacccccgt ggcctccacg gtgagttcaa tggggccgcg
     3061 atgggcttgg accacccgtt gggtcgtgcc ctccgccggc gtttcggccc tccggcgggt
     3121 gaaaaaataa aggccggcgc cgacgatcag gagggcggcg gcgatccaaa ccttgttctt
     3181 cttcattggt cctccaaagg gacgccccgg gcgttccgcc aggcggcttc ggccaccgcc
     3241 gcgtcccgct cggcctgaag agcgctgcgc tccacgttca ccaggtccgt cacgacttgc
     3301 tcccaattct caaagctcat gaggcccgag gcgtagcgaa tggacgcttc gctgttttgc
     3361 tgttcgatgg ccgccaaaaa ttgattttgc accgccaccc ggtccgcccg ggcggcccaa
     3421 tcggaccagg cgctctccag ggaccgtcga agttcgaggc gaacggcccg aaggtcctcc
     3481 tcccgggcct tccaatcgcg ccgggccgcg gcggcgtcga aaaaagcggc cgtcaatccc
     3541 ttgccgaaaa tgggccagtt caaaaccccc gaggcggtcc agt
//
LOCUS       DAOQLS010000146         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13656_length_3573_cov_3.898238, whole genome shotgun sequence.
ACCESSION   DAOQLS010000146 DAOQLS010000000
VERSION     DAOQLS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13656_length_3573_cov_3.898238"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..2266)
                     /locus_tag="PK362_03755"
     CDS             complement(104..2266)
                     /locus_tag="PK362_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNC73987.1"
                     /translation="MSPKQEILGVSTYTNGKHQAADAKLTDLFGSNVFNDRVMRKRLP
                     KNVYAAMQKVIAGETELTLDVANVVAEQMKEWAIEKGATHYTHWFQPLTGKTAEKHDS
                     FLDPQADGSAIAAFSGKTLIKGEPDASSFPSGGIRATFEARGYTAWDTTSPAFLKEDA
                     AGNVTLTIPTAFVSYTGEALDKKVPLLRSMEALSAQAIRVLRALGNKKSKRVVSTVGP
                     EQEYFLIDKDYFDRRTDLQLAGRTLFGSPSPKGQELEDQYFGAIKDRISKFMRDLDVE
                     LWKMGISSKTKHNEVAPAQFEMAPIFAISNIAADHNQLVMETMQKVALRNGLVCLLYE
                     KPFAGVNGSGKHSNWSMATDDGLNLLEPGHTPHDNEQFLVFLAATLTAVDRHADKLRA
                     GAANPGNDLRLGANEAPPAIISIFMGDSLSDILENIAKGKKTESKGQQFMTIGADALP
                     KLPMDNTDRNRTSPFAFTGNKFEFRMVPSSASIAGANTILNTIAAEALDEIADRLEKS
                     KDIEKEVNAIVREAVQKHGRVIFNGNGYSEDWVKEAAKRGLPNIKSSIEAFAAMATDS
                     AVKLFEKYKVLSGRELHSRYEIYVEQYAKHINIEARCAIQMVKNQYLPAVIAYTGELA
                     QTVNALKTAGADTKVQAALLKDVNELLASATDKLGALEEATAMANNIDNVVKKAEAFR
                     DNVFTAQADLRKDIDALETLLPADLWPVPTYAEMLFNL"
     gene            complement(2432..3175)
                     /locus_tag="PK362_03760"
     CDS             complement(2432..3175)
                     /locus_tag="PK362_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC73988.1"
                     /translation="MKKRFLWGCFLAAYPFVGRGAEAPETVFESANKHYAAGNFAGAQ
                     AAYGELAARGYAGAPLYFNLGNAYFRQGQIGRARLWYERALRESPGDEDVLYNRELLR
                     SQVGERADVRDWWADHVVLLSGLLGLANLFFFACCAGSLFRESETLWWGRWIGGVVLG
                     VLIVAGGAAHRQAARPEAVVVAARVEARTGPSAAERVGFLVPEGQRVAWLEASGEWVQ
                     IGLPEKGLKGWVRAEAVEPIDFRDPAPTG"
     gene            complement(3172..>3573)
                     /locus_tag="PK362_03765"
     CDS             complement(3172..>3573)
                     /locus_tag="PK362_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73989.1"
                     /translation="LWGTVSFRQHSLANLSGRSFRAALRRAEREVALAQKETASPAAF
                     MDRLQRIYAGFLSAKIGAPAQGLTTDRIEAAMGEAGVPAETRSRARALSDVLDRARFT
                     PGSLTPKDAGDRAAELRQLLRSLETQWKGRP"
BASE COUNT          598 a         1046 c         1193 g          736 t
ORIGIN      
        1 gtccaatggt atcgaaagcg cccctaaaaa acgaagccgc cccgcgaaga tcgcgggacg
       61 gcttcggaga aaccgatgtt ccggtcggtc ttgccggacg gttttagagg ttaaacaaca
      121 tctccgcgta ggtcgggacg ggccagaggt ccgcgggcaa cagcgtttcc aaggcgtcga
      181 tgtccttgcg gagatcggct tgggcggtga acacgttgtc ccggaaggct tcggctttct
      241 tcacgacgtt gtcgatgttg ttggccatgg ccgtcgcttc ctccagggcg ccgagtttgt
      301 ccgtggcgga ggccaggagc tcgttgacgt ccttgagcag ggcggcttga accttggtgt
      361 ccgcgccggc ggttttcagg gcgttgacgg tttgggccaa ttccccggtg taggcgatga
      421 cggcgggcag atactggttc ttgaccatct ggatggcgca acgcgcttcg atgttgatgt
      481 gtttggcata ctgctccacg tagatttcgt agcgggagtg cagttcgcgg cccgagagca
      541 ccttgtattt ttcgaagagt ttcaccgcgc tgtcggtggc catggcggcg aaggcttcga
      601 tggaggactt gatgttgggg agaccgcgct tggcggcttc cttcacccag tcttccgagt
      661 agccgttgcc gttgaagatc acgcggccgt gcttctgcac ggcttcgcgc acgatggcgt
      721 tgacttcctt ctcgatgtcc ttgctctttt cgaggcggtc ggcgatctcg tccaaggctt
      781 cggcggcgat ggtgttgagg atcgtgttgg ccccggcgat ggacgccgag gagggcacca
      841 tgcggaactc gaacttgttg cccgtgaagg cgaagggcga ggtgcggttc cggtcggtgt
      901 tgtccatggg gagcttcggc agggcgtcgg ccccgatggt catgaactgc tggcctttgc
      961 tctccgtttt tttgcccttg gcgatgttct cgagaatgtc cgagagcgag tcgcccatga
     1021 agatggagat gatggccggc ggggcttcgt tggcgcccag gcggaggtcg ttgccggggt
     1081 tggcggcgcc ggcgcgaagc ttgtcggcgt ggcggtccac cgcggtcaac gtcgcggcca
     1141 ggaacaccaa gaattgctcg ttgtcgtggg gcgtgtggcc cggttccaac aggttcaacc
     1201 cgtcatcggt ggccatggac cagttgctgt gtttgcccga cccgttgacg cccgcgaagg
     1261 gcttttcgta gaggaggcag acgaggccgt tgcggagcgc cactttctgc atggtttcca
     1321 tcaccagctg gttgtggtcg gcggcgatgt tcgagatggc gaaaatgggc gccatttcga
     1381 attgggccgg ggccacttcg ttgtgcttgg ttttggagga aatccccatt ttccagagtt
     1441 cgacgtccag gtcccgcatg aatttggaga tgcggtcctt gatggcgccg aaatactgat
     1501 cttccagctc ctggcccttg ggcgacggcg aaccgaagag ggtgcggccg gccaactgaa
     1561 ggtcggtgcg gcggtcgaag tagtccttgt cgatcaggaa atattcctgc tcgggaccca
     1621 cggtgctgac gacccgcttg gatttcttgt tgcccaaggc gcggagaacg cggatggcct
     1681 gggcggacaa ggcttccatg gagcggagca ggggcacctt tttgtccagg gcttcgccgg
     1741 tgtagctgac gaaggccgtg gggatggtga gggtcacgtt cccggcggcg tcttccttga
     1801 ggaaggccgg ggaggtcgtg tcccaggcgg tgtaaccgcg ggcttcgaag gtggcgcgga
     1861 tgccgcccga aggaaagctg gaagcgtccg gttcgccctt gatgagggtt ttgcccgaga
     1921 aggcggcgat ggcggagccg tcggcctggg gatccaggaa gctgtcgtgc ttttcggcgg
     1981 ttttgccggt cagcggctgg aaccagtggg tgtagtgggt ggcgcctttt tcaatggccc
     2041 attccttcat ctgctcggcc acgacgttgg cgacgtccag ggtcagctcg gtttcgccgg
     2101 cgatgacttt ctgcatggcg gcgtagacgt tcttgggaag gcgtttgcgc atcacgcggt
     2161 cgttgaacac attggacccg aacaaatcgg tcaacttcgc gtcggcggcc tggtgcttgc
     2221 cgttggtgta ggtggaaact ccaagaattt cttgtttggg tgacatcgta acctctcttt
     2281 tgaaaagtca ataacggtcg cgggagcgac cccaaataat cccctagtcc cgcgtcattg
     2341 gcaggtgttg ttttcggtgg acgccgttgt ccggccgtgg ttggattggt gctggttgct
     2401 gcggggttat tataccaaaa ttggccaaaa atcaaccggt cggggcgggg tcccgaaaat
     2461 cgatgggctc caccgcctcg gcgcgcaccc atcccttgag acccttttcg ggcaggccga
     2521 tttgaaccca ttcgcccgaa gcctccagcc acgccacgcg ctggccttcg ggaaccaaga
     2581 atcccacacg ctcggcggcg ctcgggcccg tccgggcctc gacccgggcg gccacgacga
     2641 ccgcttcggg ccgggcggcc tgccgatggg cggccccccc cgccacgatc aaaacgccca
     2701 agaccacgcc gccgatccac cgcccccacc aaagggtttc gctctctcga aagagggacc
     2761 cggcgcaaca ggcgaagaaa aacagattgg ccaggcccag caggcccgac aacaaaacga
     2821 cgtggtccgc ccaccaatcc cggacgtcgg ctctttcgcc cacttggctt cgaagcaatt
     2881 cccggttgta gagaacgtcc tcgtccccgg gggattcccg aagggcccgc tcataccaca
     2941 aacgcgcccg gccgatttgc ccctgccgga aataggcgtt ccccagattg aagtagaggg
     3001 gagcgccggc gtatccccgg gccgccaatt cgccgtaggc ggcctgggcg ccggcgaagt
     3061 ttcccgcggc gtaatgtttg ttggccgatt caaaaaccgt ttcgggagcc tccgcgcccc
     3121 gcccaacaaa aggataggcc gccaggaaac acccccacaa aaatctcttt ttcatggacg
     3181 tcccttccat tgggtttcca gagaccggag aagttgccga agttccgcgg cccggtcgcc
     3241 cgcgtccttc ggggtcaacg acccgggagt aaatcgggcc cggtccaaca cgtcggacaa
     3301 ggcccttgcc cggctgcggg tttcggccgg gacgcccgcc tcgcccatgg cggcttcgat
     3361 tcggtcggtc gtcaaaccct gggccggggc gccgattttg gccgaaagga aacccgcgta
     3421 aatccgttga agacggtcca tgaacgcggc ggggctggcc gtttcctttt gggccaacgc
     3481 cacctcccgc tccgcccggc gcagggccgc ccggaaggaa cggccggaca agttggccag
     3541 ggaatgctgg cggaagctca cggttcccca gag
//
LOCUS       DAOQLS010000147         3551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13799_length_3551_cov_3.966533, whole genome shotgun sequence.
ACCESSION   DAOQLS010000147 DAOQLS010000000
VERSION     DAOQLS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_3551_cov_3.966533"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PK362_03770"
     CDS             <1..841
                     /locus_tag="PK362_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC73990.1"
                     /translation="EKVKAKPESDVAVEGFLGRVGVTRLYHRAEMLRRGLQTPWSLAM
                     VEADGETAAAADRLGPLLQHEIQGGGAVGLWEDKRYVVLFLEQGMDRAEELLWALLER
                     TRRESPPILFRAGLVNLVDNLSIEEAVARAGTVLRSTHHEGPARFARFQEQSTVARKI
                     ILLAEDDELVVALVRHRLEREGFSLRCFSDGAEAFQNASAVDPHLMILDVKMPGMDGF
                     ELLERLRAEPRFERTPVLMLTSMGDESNVVKGLSLGANDYILKPFSPVELVARVHRHL
                     RAR"
     gene            846..1880
                     /locus_tag="PK362_03775"
     CDS             846..1880
                     /locus_tag="PK362_03775"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC73991.1"
                     /translation="MTSPFFFEPLLWAVGFGSLALVTAAGGLAVFAIVSRIAGARRER
                     RKARLFSKWEERWKGDRDGPWVADVRPRDARFFLEFLLSYAVALRGTSQRDLGVLADP
                     HLPWVETLLAARDPEERAWAVRALGLFGRGRRHRAVAAALGDASPLVSLSAAFALAGT
                     RDPAAAQDLLSHIDRYESWNPYFWAHAIARLGAGVTEECRRLFESPHRSGWTRRVAGL
                     ALRELRDLGSADAAARVLRHTSDGALAVVALRLLGAFGRPEHRPMVRPLVSSERPEIR
                     SLALAALSVIGNGGDADLFEDALRDPSPWVAWRGAEGLRRLRGAAALRDRLSGDSRLR
                     VLAAELDGAS"
     gene            1877..3280
                     /locus_tag="PK362_03780"
     CDS             1877..3280
                     /locus_tag="PK362_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC73992.1"
                     /translation="MNGFHIVAGGVLGYFLILNTVQLATAVIAIGSLRRYHRRFKWMD
                     AMDDFVRRGDPPSITILQPAHNEERTCVGSLRSLLTLEYASYEIIVVNDGSTDGTLDA
                     LARAFALKPVGRFPMAGIPTAAVRGTYRSAVNDKVWVIDKENGGKADALNAGINYCRT
                     ALFCAIDADCLLERDALTRIARPFIEDDCTVAVGGIVRIANGCRFRGGQVVDVRLPDA
                     LLPRFQVLEYLRAFLTGRMGWDAMNALLIISGAFGLFRRETVIEAGGFSTSTVGEDME
                     LVVRLHRHCRERRRPYRITFLPDPVAWTECPESYAALARQRDRWQRGLAQSLMRHARM
                     LFNPRYGAVGLLAFPYFFFLEMCGPVVELGGFLVFGWALWTHRLSLALSAAFGLSAFA
                     FGMALSILTVALEELSFHRYHRARDYFQLFLLALIENMGYRQLGLWWRLKGLWSAARG
                     VKRWGGNPRLGFTRGAG"
     gene            3277..>3551
                     /locus_tag="PK362_03785"
     CDS             3277..>3551
                     /locus_tag="PK362_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73993.1"
                     /translation="MKRWLFLWAVAAAPALGARDAGYWETKAGTEQQTYASGRTGWYT
                     TSLSAKRTTRRGDSLSLGVDRVTRFTQSDDAVVADTYVSVWPRAYTNL"
BASE COUNT          511 a         1102 c         1216 g          722 t
ORIGIN      
        1 tgagaaagtg aaagccaaac cggaaagcga tgttgccgtg gaagggtttt tggggcgggt
       61 gggggtgacc cgcctgtatc accgggccga gatgctccgg cgcggcctcc aaaccccttg
      121 gtccctcgcc atggtcgaag ccgacggaga gacggccgcc gcggccgacc gactcggccc
      181 cctccttcag cacgaaattc aaggcggcgg cgcggtgggc ctctgggagg acaaacgcta
      241 cgtcgtctta ttccttgaac agggcatgga tcgggcggag gaattgctgt gggcgttgct
      301 ggagagaacc cgtcgggaat cgcccccgat tcttttccgg gcgggattgg tgaacttggt
      361 ggacaacctg tccatcgaag aggccgtggc gcgggccggc acggtgttgc ggtccaccca
      421 ccacgaaggc ccggcccgtt ttgcccgatt tcaagagcaa tcgacggtgg cgcggaagat
      481 cattttgctg gccgaagacg atgaactggt ggtggccctg gttcgtcatc gattggaacg
      541 ggaaggcttt tcgcttcggt gtttcagtga cggggcggag gcttttcaaa acgcgtcggc
      601 cgtggatccc cacctcatga ttttggacgt caagatgccc ggcatggatg gttttgaatt
      661 gcttgaacgg ttgcgggccg agccccgctt cgaacggacc ccggtgctca tgttgacctc
      721 catgggggac gaatccaacg tggtgaaggg cctgtccctg ggagcgaacg attacatcct
      781 aaagcccttt tcgcccgttg aattggtggc gcgggtccat cggcatcttc gggcgcggtg
      841 atgccgtgac gtcgccgttt ttcttcgagc cgttgctgtg ggcggtgggg tttggttcct
      901 tggcgctcgt gacggcggct ggcggcctgg ccgtattcgc catcgtgtcc cggatcgccg
      961 gcgcgcggag ggaacgtcgg aaagcccggc tgttttcgaa atgggaagaa cgttggaaag
     1021 gggaccgcga cggtccctgg gtcgccgacg tgcgcccccg tgacgcccgt tttttccttg
     1081 aatttctatt gtcctacgcc gtcgcgcttc ggggaacctc tcagcgcgac ttgggggtgt
     1141 tggccgaccc ccacctccct tgggtggaaa cgcttctcgc ggctcgcgat cccgaagaac
     1201 gcgcctgggc cgtgcgggcc ctcggtcttt tcggaagggg ccgccgccac cgggcggtgg
     1261 cggccgcctt gggcgacgcc tcgcccttgg tgtccttgag cgcggccttt gctttggccg
     1321 ggacacggga tccggcggcc gcccaggacc ttctgagcca catcgatcgc tacgaaagtt
     1381 ggaacccgta tttttgggcg cacgccatcg cgcgacttgg ggcgggggta acggaggagt
     1441 gccgccggct tttcgaatcg cctcatcgtt ccggctggac gcggcgggtg gccggcttgg
     1501 ccctgcggga actgcgggat ctgggttcgg ccgacgccgc ggcccgggtt ttgcgccaca
     1561 cgagcgacgg ggccctggcc gtggtcgcgc tccgacttct cggggctttc ggccggcccg
     1621 agcaccgccc catggtgcgg ccgctcgtgt cgtcggagcg cccggaaatc cggtccctgg
     1681 ccttggccgc cttgagcgtg attggaaacg gcggcgacgc cgatctgttc gaagacgctc
     1741 ttcgggatcc gtcgccttgg gtggcctggc gcggcgcgga ggggctgcgg cgtttgcggg
     1801 gcgcggcggc cttgcgcgac cgcctgtccg gcgattcgcg cctgagggtt ctggccgcgg
     1861 aactggacgg cgcgtcgtga acgggtttca catcgtggcc gggggtgtcc tcggctactt
     1921 cttgattctg aacaccgttc aactggccac cgccgtgatc gccatcggtt ccctgcgccg
     1981 gtaccatcga aggttcaagt ggatggacgc gatggacgat ttcgttcgtc gcggagatcc
     2041 cccgtccatc acgatcctgc aaccggccca caacgaggaa cggacctgcg tcggctccct
     2101 tcggtccctt ctgacgttgg aatacgcgtc ctacgaaatc atcgtcgtca acgacggatc
     2161 cacggacggg acgctggacg ccctggcgcg ggcttttgcg ttaaaacccg tgggccgatt
     2221 ccccatggcc gggattccca cggccgccgt ccggggaacc taccgaagcg cggtcaacga
     2281 caaagtctgg gtcattgata aggaaaacgg cggcaaagcg gacgccctga acgccgggat
     2341 caattattgc cgcacggcgc tcttttgcgc catcgacgcc gattgcctgc tggaacggga
     2401 cgcgctcacc cgcatcgccc ggccgtttat cgaggacgat tgcacggtgg ccgtcggcgg
     2461 catcgtccga atcgccaacg gctgccggtt ccggggcggg caggtggtcg acgttcgtct
     2521 tcccgacgcg cttctgcccc ggtttcaggt cctcgaatat cttcgggcgt ttttaaccgg
     2581 gcgcatgggg tgggacgcca tgaacgcgct tcttatcatt tccggggcct tcggtttgtt
     2641 ccggcgggag acggtgatcg aagcgggcgg gttttccacc tccaccgtgg gggaggacat
     2701 ggaactcgtc gttcggttgc atcggcattg ccgggagcgg cgacgcccct accgcatcac
     2761 cttcctgccg gatcccgtgg cctggaccga gtgcccggaa tcctacgccg ccctcgcccg
     2821 ccagcgggac cgttggcaac gggggttggc gcaaagcctg atgcgtcacg cgcgaatgct
     2881 gttcaacccc cgctacggcg cggtcggcct gctcgctttc ccctatttct ttttcctcga
     2941 aatgtgcggg cccgtcgtgg aattgggcgg gttcctcgtt tttggctggg cgttgtggac
     3001 ccaccggctg tccttggcgc tctcggcggc tttcgggttg tcggccttcg ctttcggcat
     3061 ggcgctgtcg atcttgacgg tggcgctgga ggagctttcc tttcatcgat accaccgggc
     3121 ccgggattat ttccagctgt tcctcttggc cttgatcgaa aacatgggct accgccaact
     3181 gggcctgtgg tggcgcctga aaggcctttg gtccgcggcc cggggcgtta agcgatgggg
     3241 ggggaacccc cgtttgggat ttacgcgggg ggcggggtga aacgttggtt gttcctctgg
     3301 gccgtggccg cggctccggc cctcggggcc cgcgacgccg gttattggga aaccaaggcc
     3361 ggcaccgaac agcagacgta cgcctctggc cggaccggtt ggtacacgac ctccctgtcc
     3421 gcgaaacgaa cgacgcgacg cggggattcc ctttcgctgg gcgtggaccg ggtcacgcgc
     3481 ttcacccaaa gcgacgacgc cgtggtcgcg gacacctacg tcagcgtgtg gccccgggcc
     3541 tacaccaacc t
//
LOCUS       DAOQLS010000148         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13827_length_3545_cov_4.481089, whole genome shotgun sequence.
ACCESSION   DAOQLS010000148 DAOQLS010000000
VERSION     DAOQLS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13827_length_3545_cov_4.481089"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK362_03790"
     CDS             <1..208
                     /locus_tag="PK362_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73994.1"
                     /translation="RPVAVSPIGLERFFSSRGREVLRSEAVWLILPSFLVFGVATILR
                     GTDRRPRKNPTTKYEANPCPPSSL"
     gene            187..729
                     /locus_tag="PK362_03795"
     CDS             187..729
                     /locus_tag="PK362_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73995.1"
                     /translation="MPPIVFVKSASGPVPIDVFLVAQPSWVQKGLGAAARELSASHQI
                     LGVRAHERKNGEWSVWLEDEKGERHNFILYLSPLALKDPSVPAATGGKNPRLGVYREY
                     GHAQGSGVEFLEAEAFIRDHFPGDVRERLLAALQRAREKLPSFDGSIEWTRTGVVLRA
                     VNQNTQVRAFDAELRLEWER"
     gene            732..1529
                     /locus_tag="PK362_03800"
     CDS             732..1529
                     /locus_tag="PK362_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC73996.1"
                     /translation="MPGTIRFEDGAAYERYMGKWSQSVAVRLLDWLAPPPGWRWLDVG
                     CGNGAFTEILGERCAPAALHGIDPSDAQLAFARSRPVARFARFIAGNAMALPFPEDRF
                     DAAVMPLVISFVPRPATGVGEMTRVVRPSGLVAAYIWDTAGGGFPYETLHNELRSRGV
                     AVPAPPSPEASRKEVLGALWAEAGLVDVETRVFEVQRIFENFEDYWKTIFGAPSVGST
                     LAGMGAAELARLNAGLREKLPVESDGRLILKARANAVRGIVPGKRRA"
     gene            1822..2208
                     /locus_tag="PK362_03805"
     CDS             1822..2208
                     /locus_tag="PK362_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HNC73997.1"
                     /translation="MRRRIRTLAIVLIFPAAVAGAADPFSLEYHACMDKAGGVTFDTL
                     HCIADETKRQDVRLNTAYQVLMKGLPEPRKTQLRDVQRDWIRYRKSNYDFYLDPDGGT
                     LASVLSADCFLSATAERARELENFNP"
     gene            2282..2650
                     /locus_tag="PK362_03810"
     CDS             2282..2650
                     /locus_tag="PK362_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC73998.1"
                     /translation="MAGVLPLFAAPQKCPLAVETKQSIQTTPAGWTVLQTPAPSRLTR
                     VAIFDGPPREEASLKPDNGDTDEAPVWTLSPDNPRGYWLVCYYDDTSIALSKRLPGQV
                     SNCRAVLDRYSALREVECRP"
     gene            2841..3212
                     /locus_tag="PK362_03815"
     CDS             2841..3212
                     /locus_tag="PK362_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC73999.1"
                     /translation="MAKTTTTYWNPLDPRNRGRWTPVKGIESMAEELTLSVDDKTREY
                     TRLTRFFPGADTRPFGGKSHPYPEEVFIVSGRLFDHAFGRWLEPGDYASRPPGEIHGP
                     FTTETGCVVLEISFPSRTGNG"
     gene            3209..>3545
                     /locus_tag="PK362_03820"
     CDS             3209..>3545
                     /locus_tag="PK362_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74000.1"
                     /translation="MTTPAIRRRRVFLGTLLLPVALVLGGVRPRPKTWAVPLKVQGFH
                     NLHRVNGDLYRCAQPSSAAFAELAARPVLQNGDRPVKTVLSLRAFNDDEPRAPADAPF
                     QLENIRFNTW"
BASE COUNT          661 a         1151 c         1079 g          654 t
ORIGIN      
        1 tcgccccgtc gctgtttccc ccatcggcct ggagcggttt ttttcctccc ggggacggga
       61 ggttctacga agcgaagcgg tttggttgat ccttccctct tttttggtgt ttggggtcgc
      121 cacgattctc cgtggaacgg accgccgtcc acggaaaaat ccaaccacta aatacgaggc
      181 gaacccatgc cccccatcgt ctttgtgaaa tccgcgtcgg gccccgtgcc catcgacgtt
      241 ttcctagttg cacaaccgtc ctgggttcaa aaaggcctgg gcgccgcggc gcgggaactg
      301 tcggcgtccc atcaaatcct cggcgtccgc gcccacgagc gtaaaaacgg cgagtggtcc
      361 gtctggctcg aggacgaaaa aggcgagcga cacaatttca tcctttattt aagccccctg
      421 gcattaaaag acccctccgt tcccgccgcg acgggcggga aaaacccgcg gttgggagtt
      481 tatcgagaat acggccacgc ccagggaagc ggcgtggaat tcctggaggc cgaggctttc
      541 atacgtgacc attttccggg cgatgtccgg gaacggcttt tggcggccct tcaacgggcg
      601 cgggaaaaac tcccgtcttt tgacgggtcc atcgaatgga cccgcaccgg ggtcgttctt
      661 cgggcggtca atcaaaacac ccaggtgcgg gccttcgacg ccgaattgcg cttggaatgg
      721 gaacgatgac gatgcccggc acgatccgtt ttgaagacgg agccgcctac gaacgttaca
      781 tggggaagtg gagccaatcg gtcgccgtgc gccttctcga ctggctcgct ccgcccccgg
      841 gatggcgttg gctggacgtg ggatgcggca acggcgcctt caccgaaatc ctgggagagc
      901 ggtgcgcccc ggcggcgctg cacggcatcg acccctccga cgcccagctg gcgttcgccc
      961 gctcccggcc cgtggcccgt ttcgcccgat tcatcgccgg caacgccatg gccctgcctt
     1021 ttccggagga ccgctttgac gcggccgtga tgcccctggt gatctctttc gtcccgcgac
     1081 cggccaccgg ggtcggcgaa atgacccggg tcgtgcgccc gtccggactg gtcgcggcgt
     1141 atatctggga cacggcgggc ggcggtttcc cctacgagac gcttcacaac gagctgcgtt
     1201 cccgcggggt ggccgttccg gccccgccga gcccggaggc ttcccgaaag gaagttctcg
     1261 gcgccctgtg ggcggaggcg ggactcgtgg acgtcgaaac ccgtgtcttt gaggttcagc
     1321 ggatttttga aaatttcgag gattactgga aaacgatttt cggagcgccc agcgtgggct
     1381 cgacgctggc ggggatgggg gccgcggaac tcgcccggct caacgccggt ttgcgggaga
     1441 aactaccggt cgaatccgac ggtcgtctga ttcttaaagc cagggccaac gcggtgcggg
     1501 gaatcgtccc cggaaagcgg cgggcttgac gggtccccgc cgcgggttgg tcggggtgga
     1561 aaaaccccgg ggcttccatt gacctacaac gacgcccggg aaaagcaata cgaaagggaa
     1621 gtacggcgga acgcttaccg ccttggaatc gagattcgag tggaaaacgg ggaatgtttc
     1681 ctgctggaag agggccgcgg gcacccgctg tgtcgccccg acctgccgaa acgattttgg
     1741 tatgagacat ggttggcgct taagcggcga tatccgcttt tgccgaaatt cattcgataa
     1801 tttgcttcaa gggagggatg gatgcgtcgt cggattcgaa cgctcgcgat cgttttgatt
     1861 ttccccgccg cggtcgcggg ggcggccgat cctttttctt tggaatacca cgcgtgcatg
     1921 gacaaagccg gcggggtcac gttcgacacc cttcactgca tcgccgacga aacaaaaagg
     1981 caagacgtgc ggcttaacac ggcgtatcaa gtcctgatga aaggccttcc ggagccccgc
     2041 aaaacccaat tgcgcgacgt ccaacgcgat tggattcgct accgcaaatc aaattacgac
     2101 ttttatctcg acccggacgg cggcaccttg gcctcggtcc tctcggccga ttgcttcctt
     2161 tcggccacgg ccgagcgggc ccgggaactc gaaaatttta atccgtaatc accgaggcgc
     2221 cccacccagg agacccccga tgccctcaac cacaacaacg acaatcgccg ccggactgtg
     2281 gatggcgggc gtccttcctc ttttcgccgc cccgcaaaag tgccctttgg ccgtcgagac
     2341 aaaacaatcg atccaaacga cgccggcggg ctggaccgtc ctccaaacac cggccccctc
     2401 ccggctgacc cgggtggcca tatttgacgg cccgccgcgg gaagaggctt ccctcaagcc
     2461 ggacaacggg gacaccgatg aagcgccggt gtggaccctg tcgccggaca acccccgggg
     2521 gtattggctc gtttgttact acgacgacac gagcatcgcc ctgtcaaaac gattgccggg
     2581 gcaggtatcg aattgccggg ccgttttgga ccggtattcc gccctccgcg aggtggaatg
     2641 ccggccctaa ggcgattccg tcgcgcactc cccgatcggg aaatccttcg accgtcggtt
     2701 ccccccgggg ttctttcgcc gtcgaggacc gtgaaggggg ttggcgggac agggaccgtt
     2761 cggcgccgac gacccgcggc gggcatcgca accgccaccg gaaggccgtt caaaaggtaa
     2821 aatcccgaga ggacaattcc atggccaaaa caacgacgac ttactggaac cctttggacc
     2881 cgcgaaatcg cgggcgctgg acccccgtga aggggatcga atcgatggcg gaggaattaa
     2941 cgctcagcgt cgacgacaaa acccgcgaat acacccggct gacccgcttt ttcccggggg
     3001 ccgacacccg gcccttcggc ggcaagagcc atccctatcc cgaagaagtt ttcatcgtca
     3061 gcggccgact tttcgaccac gcctttggcc gctggctgga gcccggcgac tacgccagcc
     3121 gccccccggg cgaaatccac ggccctttca ccaccgaaac gggttgcgtg gtgcttgaaa
     3181 tatctttccc ctcccggacc ggaaacgggt gacaaccccc gcgattcgac gccgccgggt
     3241 gtttctggga acgctgctgc tcccggtggc cctggtcctg ggcggcgtcc gcccgcgacc
     3301 caaaacctgg gccgtccccc tcaaagttca agggttccac aacctgcacc gggtgaacgg
     3361 cgacctttat cgatgcgccc aacccagctc ggcggccttc gccgagctgg cggcgcgccc
     3421 cgtccttcaa aacggggacc gtcccgtcaa aaccgttctt tccctgcggg ctttcaacga
     3481 cgacgaacca cgggcgccgg ccgacgcccc ctttcaattg gaaaacattc ggttcaatac
     3541 ctggc
//
LOCUS       DAOQLS010000149         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13946_length_3525_cov_4.654467, whole genome shotgun sequence.
ACCESSION   DAOQLS010000149 DAOQLS010000000
VERSION     DAOQLS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13946_length_3525_cov_4.654467"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="PK362_03825"
     CDS             complement(<1..1902)
                     /locus_tag="PK362_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74001.1"
                     /translation="MTKIKAVLRFLRPLGPSSEGLRSILGGLLAVSFFFCNVLGVYAQ
                     EQNFWSARRMAARKMQPPESEGIDLGQEGNMLLSQIPQAVRMDLGSAGGAAISSAPGE
                     LAKIPATKSAPQDWLAKLVTPYGWVRDVYLSPKANAPVVVHIQDAHGIEEAQRNISAM
                     LGSLMQERRVNLVGLEGASGSFTLDPYRNYPDASITKDIADFLLIKGLIAGPEYAGLT
                     LPQAPAFYGAEDSTLYEGNVKALKTAFKTKTALLATLATLRAAAEQEKKKTFSEGLKS
                     FDRHFEAYKAQREKLGDYVRALVKTPGGRDARLDIPNVRLLLNALDWEENLDFKAVET
                     ERQRLVEVLVRRLSKPALQDLISRSIDYRTGRMGYGDYHRHLRQVCADNKIRLDDFGQ
                     LSSYITYVLLAEKISRNELVAELDQLEIAAQNSLAKTPDETALMNIARDLALLEKMLR
                     HEMAMPDWLAYEKRRNEILALRDRLAALDPTVAKLPSLTVETLKPFEDFCSYAVRRND
                     ALVANLLAKMAEKGTTTGVLVAGGFHTEGLTGLFRQKQISYVVVTPKITDVPGESDYL
                     DILAHDPVPLEKLVAGDRIYLSHPIALADAAGETPKFIHGAQEVLQNGVRSVSVSAAE
                     AVEPCDNVHG"
     gene            complement(2018..2362)
                     /locus_tag="PK362_03830"
     CDS             complement(2018..2362)
                     /locus_tag="PK362_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNC74002.1"
                     /translation="MLETKALFDVTERAAQRAKKLLAEKGQGAEALRVKVTAGGCSGF
                     SYSLEPLVGPAPQTDHRVDAHGVTVYVDKKSLLYLAGTTLDYEDKMFSRRFLFKNPNA
                     TATCSCGESFGV"
     gene            complement(2369..2692)
                     /locus_tag="PK362_03835"
     CDS             complement(2369..2692)
                     /locus_tag="PK362_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNC74003.1"
                     /translation="MLTMTPNAVEKVKEFYANDKELAGKALRVFVEKGGCSSYQYGFT
                     FDDKKDGDTALDLSGLNLVIDGTSAALLAGAVIDYKEDFSGGGFAISNPNAKKSCGCG
                     KSFDA"
     gene            2780..3310
                     /gene="def"
                     /locus_tag="PK362_03840"
     CDS             2780..3310
                     /gene="def"
                     /locus_tag="PK362_03840"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC74004.1"
                     /translation="MSTAAESPTLLEIRKFGDPVLQRPAKPVEKIDEKLLRLIAAMFR
                     TMYAEPGIGLAAPQVGVSQRLMVLDVAPEGQSRPLVMINPFFVEKKGKIRSEEGCLSF
                     PGIQVTVPRAARVKVSGINERGLPVTVEAEGLLSRCLQHEMDHLDGVVMIDRLRLPQR
                     LKALWDIRQRKKAGLW"
     gene            3327..>3525
                     /locus_tag="PK362_03845"
     CDS             3327..>3525
                     /locus_tag="PK362_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74005.1"
                     /translation="MTDPAPAAPPSALLKMVRASRHLITAVCVVLVLVTVVSLFRSAL
                     GPSGSWRLLAESGQVFLPAVFL"
BASE COUNT          633 a          995 c         1144 g          753 t
ORIGIN      
        1 cccgtgaacg ttgtcgcagg gctccacggc ctcggcggcc gagacggaaa cggatcggac
       61 gccgttttga aggacctcct gggcgccgtg gatgaatttc ggggtttcgc ccgcggcgtc
      121 ggccagggcg atggggtgcg ataaatagat gcggtcgccg gcgaccaatt tttccagggg
      181 cacggggtcg tgggccagga tgtccaggta atcgctttca ccgggaacat cggtgatttt
      241 gggcgtgacc acgacgtagg aaatctgttt ttgccggaag aggcccgtca gcccttcggt
      301 gtggaagccg cccgccacca acacgcccgt cgtggtccct ttttcggcca ttttggccag
      361 caggttggcg accagggcgt cgtttcggcg gacggcgtag gagcagaaat cttcaaaagg
      421 tttcagcgtc tcgaccgtga gggaggggag tttggccacc gtggggtcca gggcggccag
      481 gcggtcccgc agggccagaa tctcgttgcg gcgcttttcg tacgccagcc agtcgggcat
      541 ggccatttcg tgccggagca ttttttccaa aagcgccagg tcccgggcga tgttcatcaa
      601 ggcggtttca tcgggggttt tggccaggga gttttgggcg gcgatttcca gctgatccag
      661 ttcagcgacg agctcgttgc ggctgatttt ttcggccagc aggacgtagg tgatgtaact
      721 ggaaagctgg ccgaaatcgt ccagccggat tttattgtcg gcgcaaacct ggcgcaggtg
      781 ccgatggtag tcgccgtagc ccatccggcc cgtgcggtag tcgatgcttc gggagatcag
      841 gtcctggagc gccggcttcg acaaccgtcg aacgaggact tccaccaggc gctggcgctc
      901 ggtttccacg gctttgaagt cgaggttctc ctcccaatcc agggcgttca acagcagccg
      961 gacgttggga atgtccaggc gcgcgtcccg gcccccgggg gttttgacca gggcgcgaac
     1021 gtaatcgccc agtttttccc gctgggcttt gtaggcctca aaatgccggt caaagctttt
     1081 gaggccctcg gagaaggttt ttttcttttc ctgttcggcg gcggcccgca gggtggccag
     1141 ggtggccaga agggcggttt tcgttttgaa agcggtttta agggctttga cgttcccttc
     1201 gtagagggtg gaatcttccg cgccgtaaaa ggccggggct tgagggaggg tgaggccggc
     1261 gtattcgggc ccggcgatca atcctttgat taaaagaaaa tcggcgatgt ccttggtgat
     1321 ggaggcgtcc ggataattcc ggtaggggtc cagggtgaag gagcccgagg cgccctccag
     1381 gcccaccagg ttgacccggc gttcctgcat gagggacccc agcatggcgg agatgttccg
     1441 ctgggcctcc tcgattccgt gggcgtcctg gatgtgaacg accaccgggg cgttggcctt
     1501 gggggaaagg tagacgtccc gcacccaacc atagggggtg accagcttgg ccagccaatc
     1561 ctgaggagcg gacttggtgg cggggatctt ggccaattcc ccgggggccg atgagatcgc
     1621 ggctccgccg gcggaaccca aatccattcg gaccgcctgg gggatttggg agaggagcat
     1681 gttgccctct tgccccagat cgatcccctc ggattccggc ggctgcattt tgcgcgcggc
     1741 catccggcgg gcgctccaaa aattttgttc ctgggcgtag acgccgagta cgttgcagaa
     1801 gaaaaaggag accgccaaaa ggccgcccaa aatggaccga aggccttccg aggaagggcc
     1861 gagggggcgt agaaagcgaa gaacggcttt gattttcgtc atgggttggc cattttagag
     1921 gggaggattt ccctcgacgt ggcttaatag aagaataagg gatgaagggc gaaaaagccc
     1981 ttaagaggtg gtaaatttat tgtaacgggt ttttgggtta aacgccgaag gattcgccgc
     2041 aggaacaggt ggcggtggcg ttggggttct tgaagagaaa acggcgggaa aacatcttgt
     2101 cttcgtagtc gagggtcgtg ccggccaggt aaagcagact ttttttgtcc acatagacgg
     2161 tcacgccgtg ggcgtccacc cggtggtccg tttggggggc cgggccgacc aggggttcca
     2221 gggaataact gaaaccggag cagccgcccg ccgtgacctt gacgcgcagt gcctccgcgc
     2281 cctggccctt ttcggccaga agttttttgg cgcgctgggc ggcgcgctcg gtcacgtcaa
     2341 agagggcttt ggtttcgagc acggggcttt aggcgtcgaa ggatttgccg caaccgcagc
     2401 ttttcttggc gttggggttg gagatggcga acccgccgcc ggaaaaatcc tccttgtaat
     2461 cgatgacggc gcccgccaag agggcggcgc tcgttccgtc gatcacaagg ttcaatccgc
     2521 tcaggtccag ggcggtgtcg ccgtcctttt tgtcatcgaa ggtgaacccg tactgatagc
     2581 tggagcagcc gcctttttcc acgaagaccc gcagggcttt tcccgcgagt tctttgtcgt
     2641 tggcgtaaaa ttctttgacc ttttccaccg cgttcggggt cattgtgagc atggttcctc
     2701 cggggttcct gatatccctt taattatgcc aaaaaagtca tatattgtca acgacggaaa
     2761 agagcggtat aatattccca tgtccaccgc cgccgaatcc ccgaccctcc tggaaatccg
     2821 caaattcggc gaccccgtct tgcaacggcc cgccaagccc gtggaaaaga tcgatgagaa
     2881 acttctccgc ttgatcgcgg ccatgttccg gacgatgtac gcggagcccg gcatcggcct
     2941 ggccgccccc caggtggggg tgtcccaacg tttgatggtc ttggacgtcg cccccgaagg
     3001 ccagagccgt cccctcgtca tgatcaaccc gttcttcgtt gagaaaaaag gaaaaatccg
     3061 gtccgaagaa gggtgtttgt cctttccggg aatccaggtg acggtgcccc gggcggcccg
     3121 ggtgaaggtg tcgggcatca acgaacgcgg actgccggtg accgttgagg ccgagggtct
     3181 cctgtcccgg tgccttcagc acgaaatgga ccatttggac ggggtggtga tgatcgaccg
     3241 ccttcgtctc ccccagcggc tcaaagcgct ctgggacatt cgccagcgta aaaaagccgg
     3301 gctctggtag cccccaagga cctcccgtga ccgaccccgc tcccgccgcc ccgccttccg
     3361 cgctcctaaa gatggttcgc gcctcccgcc atctcatcac cgccgtctgc gttgttttgg
     3421 ttttagtcac ggtggtttcc cttttccgaa gcgccctcgg cccgagcggc tcctggcgtc
     3481 tcctggccga aagcggccag gtgttcctgc ccgccgtttt ccttt
//
LOCUS       DAOQLS010000150         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_13950_length_3525_cov_3.967147, whole genome shotgun sequence.
ACCESSION   DAOQLS010000150 DAOQLS010000000
VERSION     DAOQLS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13950_length_3525_cov_3.967147"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PK362_03850"
     CDS             complement(<1..768)
                     /locus_tag="PK362_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNC74006.1"
                     /translation="MNAGWIEKLRRTLGDDVVRTAPGDRFAYAFDAALEKRLPGAVVL
                     PRNAEDVARAVRIAVDHGVPYVARGAGTNLCGGAVPSTGGLVVHLARMNRVLSIDRER
                     RRAWVEPGVVNLHLHALLAPLGLFYAPDPASQKACTLGGNVGTNAGGPHCLKYGVTST
                     HVTGLEMVLPDGGTGRFSVDDAGYDLTGLFVGSEGTLGVATKIELNLMPAPEAVETFL
                     VAFPSMEAAAQTVTDTISAGIVPATLEVMDRLTVDAVE"
     gene            851..1387
                     /locus_tag="PK362_03855"
     CDS             851..1387
                     /locus_tag="PK362_03855"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNC74007.1"
                     /translation="MKRYLLTAAGGDRPGLVAAVTRSLFDHGCNLEDSAMTRLQGEFA
                     ILLIFSGPPSTAPLEKTLKRLGKKTGLVTHVKSLSGRETRSPRRAAEGVLVSVYGADR
                     PGIVFHVTDRLARAKVNITDLSTHRTSAGKSAGYILYIEAELPRGLSPEALQSTLRDG
                     AAAWGVTVQVKPLAASAL"
     gene            1397..1918
                     /gene="def"
                     /locus_tag="PK362_03860"
     CDS             1397..1918
                     /gene="def"
                     /locus_tag="PK362_03860"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC74008.1"
                     /translation="MAVRPILLFPDDGLKKACAETPGVDGVVAQLLTDLTDTLYASPG
                     VGLAAPQIGSLLRAVVVDVTRYPPRKGQPPVHGHGLLVLVNPKIVKREGELVFREGCL
                     SIPEFLADIRRSAAVRVEALDRAGAPVAIDAEGFEAVALQHEMDHLDGLLFLDRVANL
                     KTDLFRRKPPSRS"
     gene            complement(2003..2944)
                     /locus_tag="PK362_03865"
     CDS             complement(2003..2944)
                     /locus_tag="PK362_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74009.1"
                     /translation="MTSFKAVPLAAMLLAAGTTLHAANAGSDLFIDAGTLAHWFSEQL
                     SNASVFSAAGSLPRPGAVHDLLGVEVGLSGAVTSTPLDVDGFHGLPLTRYDGTTVDLP
                     SRLLMPVPVVHLKVGIPGGFDVGMRFGSANSNENTNDAKSEFKNQVVGVEVRRRLLGG
                     GLTGVALPDVALGLSYDRSTGKVDRTEVYNGPLNGGGSLNATTHWKSEWNTSALALRA
                     TASKTLLMFTPYLGVGYAKYMGDTDTSINIAGTVSDPAPTAIDQTSTATAKPRSGEAQ
                     VTGGVEIAFFPLVHLNVGGVVGQKDWGANVGLVVGFR"
     gene            complement(2978..>3525)
                     /locus_tag="PK362_03870"
     CDS             complement(2978..>3525)
                     /locus_tag="PK362_03870"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNC74010.1"
                     /translation="LGRPALFVGPGVVTGVRVLVDAPAEVGADRIVNAAAAFARKRRA
                     CIVVDFGTATTFDCVSAGGDYRGGVIAPGPVMAAEGLARRTAKLPHVGVFRRPARALG
                     TNTLDSIAAGLYHGYVGLTREILRRLLAEMKGRPAILATGGLAPLIGPEIGDIKEIVP
                     DLTLEGLWLIWRLNQGKHRGA"
BASE COUNT          630 a         1194 c         1112 g          589 t
ORIGIN      
        1 ctccacggcg tccacggtca atcggtccat gacttccaag gtcgccggca cgatgccggc
       61 ggaaatcgtg tcggtgacgg tctgggccgc cgcctccatg gagggaaagg cgaccaaaaa
      121 cgtctccacc gcttccggcg cgggcatcag gttcaattcg attttggtcg cgacgcccaa
      181 ggttccctcg gaccccacga acaatcccgt cagatcgtaa ccggcatcgt cgacggaaaa
      241 gcggcccgtc ccgccgtccg gcaggaccat ttcaagcccc gtcacgtggg tggaggtcac
      301 gccgtatttc agacaatggg gtcccccggc gttggttccg acgttcccgc ccaaggtgca
      361 ggctttttga ctggccgggt ccggagcgta aaagaggccc aacggcgcca agagggcgtg
      421 caaatggagg ttgaccacgc cgggttcgac ccaggcgcgc cggcgctccc ggtcgatgga
      481 caaaacgcgg ttcatccggg ccaggtggac cacgaggccg ccggtggacg ggacggcgcc
      541 gccgcaaagg ttggtgccgg cgccccgggc cacgtagggc acgccgtggt cgacggcgat
      601 ccgcacggcg cgcgccacgt cctccgcgtt ccggggcaga accaccgcgc cgggcagccg
      661 tttttccaaa gcggcgtcaa aggcgtaggc aaaccgatcc ccgggagcgg tccgcacgac
      721 atcgtcgccc agcgtccgcc gaagtttttc gatccaaccg gcattcatgg agaatccatt
      781 gtaccctttt tgaggcggcc atcgagggcg cggcgttgcg cctccgacgg aaatgtggta
      841 ataaatcccc gtgaaacgct acctattgac cgccgcgggc ggcgaccgtc cggggttggt
      901 ggccgcggtg acccgatcgc tgtttgatca cgggtgcaat ctggaagaca gcgccatgac
      961 ccggctccag ggcgaattcg ccatcctgtt gattttttcg ggacccccct ccacggcccc
     1021 gctggaaaaa accctgaagc gcctcggaaa gaaaacaggg ctggtgaccc acgtcaaatc
     1081 cctttccggc cgggaaacgc ggtcgccccg gcgggcggcg gaaggcgtgc tcgtctcggt
     1141 gtacggcgcc gatcgtccgg gcattgtgtt ccacgtcacg gaccggctgg cccgggccaa
     1201 ggtcaacatc accgatctct ccacccaccg cacgagcgcc ggcaaaagcg cggggtacat
     1261 tctttacatt gaagccgaat tgccccgggg gttgtcgccc gaggcgctgc agtcgaccct
     1321 tcgggacggc gccgccgcct ggggcgtcac cgtccaagtc aaacccctgg ccgcttcggc
     1381 cctttagcct tcccgcgtgg ccgtccgccc gattctcctt tttcccgacg acgggttaaa
     1441 aaaagcctgc gccgaaaccc cgggcgtcga cggagtcgtc gcgcagctgt tgaccgacct
     1501 gacggacacc ctgtatgctt cgcccggcgt gggtttggcc gcgcctcaaa tcggttccct
     1561 ccttcgggcg gtggtggtgg acgtgacccg gtaccccccg cgaaaggggc agccgcccgt
     1621 ccatgggcac ggactattgg ttctcgtcaa tccgaaaatc gtgaaacggg agggggagct
     1681 ggtgtttcgg gaaggctgct tgtccattcc ggaattcctg gcggacatcc ggcgttcggc
     1741 cgccgtgcgc gtcgaagccc tcgatcgcgc gggagccccc gtggccatcg acgccgaggg
     1801 attcgaggcc gtcgcccttc aacacgaaat ggatcatctg gacggactgc tgttcctcga
     1861 ccgggtcgcc aacctaaaaa ccgacctctt ccgccgaaag cccccctccc gctcgtaagc
     1921 gggcctttcg accgtcgggc ggtttcccca taaaaaaagc ccccctcccc ggaccgggga
     1981 ggggggcttt aaaaatcagc tcttaacgga aaccgacgac caaaccgacg ttggcgcccc
     2041 aatccttctg ccccaccacg ccgcccacgt tcaaatgaac cagcgggaaa aaggcgatct
     2101 cgacgccgcc cgtcacctgc gcctcccccg atcggggctt ggcggtggcc gtggaggtct
     2161 gatcgatggc cgtgggcgcg ggatccgaca cggtgccggc gatgttgatg gaggtgtcgg
     2221 tgtcgcccat gtatttcgcg taacccaccc ccagataggg ggtgaacatc aaaagcgttt
     2281 tggaggccgt ggcccgaagg gcgagggccg acgtgttcca ttcggatttc caatgggtcg
     2341 tggcgtttaa cgaaccgccg ccgttgaggg gtccgttgta gacctcggtg cggtccacct
     2401 tgccggtcga gcggtcgtag gagagaccca acgccacgtc gggcagggcc accccggtca
     2461 atccgccgcc cagaagccgg cgacgaactt ccacccccac cacctggttt ttaaattcgc
     2521 ttttcgcgtc gttggtgttc tcgttggaat tggccgaccc gaagcgcatt ccgacgtcga
     2581 accccccggg aatccccact ttgaggtgga cgaccgggac cggcatcaac aggcggctcg
     2641 gcaggtcgac cgtcgttccg tcgtagcggg tcaaaggcag cccgtggaat ccgtcgacgt
     2701 ccagcggcgt cgacgtcacc gccccggaca atccgacttc aacccccagc agatcgtgta
     2761 cggccccggg gcgcggcagg gaacccgccg cgctgaaaac gctcgcgttg gacagctgct
     2821 cgctgaacca gtgggccaga gtcccggcgt cgatgaacag gtcggagccg gcgttcgcgg
     2881 cgtggagcgt cgttccggcg gccaaaagca tcgccgccag cggaaccgct ttaaaactcg
     2941 tcattcgcat agtgccctcc cggggttgcc gcgggcctta ggcgccgcgg tgttttcctt
     3001 gattcaatcg ccaaatcaac caaagccctt ccagggtcaa gtccggaaca atctccttta
     3061 tatccccgat ttccggcccg atcaaggggg cgaggccacc ggtggcgaga atggccggac
     3121 ggcctttcat ttcagcgaga aggcgtcgaa gaatttcccg ggtcagaccc acgtagccgt
     3181 gataaagacc ggcggcgatg ctgtccaagg tgttggtgcc gagcgctcgg gccggccgac
     3241 gaaaaacgcc cacgtgcggc aacttggcgg tgcggcgggc caaaccttcg gcggccatca
     3301 cgggccccgg agcgatcacc ccgccgcggt agtccccccc ggcggacacg caatcaaagg
     3361 ttgtcgcggt tccaaaatcc acgacgatgc acgcccgccg cttccgggcg aaggccgccg
     3421 ccgcgttaac gattcgatcg gcccccactt ccgccggggc gtccaccagc acccggacgc
     3481 cggtcaccac gccgggcccg acaaagaggg ccggccggcc caggt
//
LOCUS       DAOQLS010000151         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_14016_length_3515_cov_3.201734, whole genome shotgun sequence.
ACCESSION   DAOQLS010000151 DAOQLS010000000
VERSION     DAOQLS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14016_length_3515_cov_3.201734"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PK362_03875"
     CDS             <1..184
                     /locus_tag="PK362_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009545613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74011.1"
                     /translation="RERCDDLIAVPGEAKVPSLNASCASAALLYELYRRRGPDPRSGV
                     GGPLPGSGSGNGGNPR"
     gene            181..2295
                     /locus_tag="PK362_03880"
     CDS             181..2295
                     /locus_tag="PK362_03880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC74012.1"
                     /translation="MTVPPAPSAPRRRGVFFLAAVALTAVQAAALWSWLRWDVRPPGE
                     SAAALLLEARRVETDLRAGRLLDWLEARPGGGTSQQPPLVPALLSVGLSLGRSLGTDP
                     ADAAVWVVGLLSIVFLSMGAYRFAFPVGGAEAGLAAAALASTVPFFLTWTRRPAPDAA
                     LAAAVVYAHGLWVATEGFTRFRRTVALGVALGVGVLVRWTFLLYVAPVIAAAAWTFFV
                     RRKNKRRLLLGFLAAVAVAGPWYVLNATVLIPAAVAFFRTRSGAGPFPAGWFWPVFSG
                     FPALLWPLAGGAGLLWAFLRRRRFFGRPVAWLSTGLAAVLFCPLKEARVLLPAALALP
                     VAAAALPFHVPAVLAAGALTATAWSTWGPDSARRSVPDPSALDAIVDRVRERLPTGDS
                     PPVLTLVARDALLNEPSLAWGFEERGLSDRIEIQTRLDRLGQFAEFLLVKTAPAGASG
                     AGEAWEKARDEVLNPAGWFRRGFNEAGRWPLPDGTSAVLLQRSTQSVAMEGPSGLAGI
                     LPGLGDGISLEGFQGSLEPSLRYPGQSMCRLSTDRLTVGAWSIGRVQLLLDGLVLARD
                     DAGQPRLLDLHRLELVSARWTAADAERLLAAQAPVLRDPRVRFLAENRVEVSGRCWGF
                     PLRLRAAVATESVPGPALEARVERFSVGPVPVPVLALGPWRKRRLALSPGGRLPVRIR
                     FPALRTVPADESSGGYLEWGAP"
     gene            2292..3269
                     /locus_tag="PK362_03885"
     CDS             2292..3269
                     /locus_tag="PK362_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC74013.1"
                     /translation="MTSMELSVIVPVYNEERVIEVFHRALVEVLDGLDFPCEIIYVND
                     GSRDQTGDRLDALRASDSRVRLLELSRNFGHQSALAAGLSRASGRACVFMDADLQDPP
                     ALLPRLVERWRAGVEVVYAVRRARPGDSLFKRWTARLFYRGLGLIAGVSLPPDAGDFR
                     LLDRKVVDALIALPERHRYFRGLVSWVGFRQEGVPFDRPARAAGETKFTLWKMIRFAV
                     DGVTSFSHVPLRLVTLAGFAASAGSAVLLAWAFYVKFVAHRTVQGWTSLIAVVLLMGG
                     AQFLALGILGEYLARVLDETKGRPLFLVRRAAGFGEAAADRPPRPESRA"
BASE COUNT          397 a         1182 c         1213 g          723 t
ORIGIN      
        1 ccgcgagcgt tgcgacgatc tgatcgcggt gccgggggaa gccaaagttc cttccttaaa
       61 cgcttcctgc gcctcggcgg ctctcctgta cgagctttat cggcggcgcg ggccggaccc
      121 ccgttccggc gtcgggggcc ctctccccgg gtcggggtcc gggaacgggg ggaacccccg
      181 gtgaccgtgc ctccggcgcc ctcggccccg cgccgacgcg gggtcttttt tttggccgcg
      241 gtcgcgttga ccgcggtgca agcggccgcg ctctggtcgt ggctgcgatg ggacgttcgt
      301 ccgcccgggg aatccgccgc ggctttgttg ctggaagccc gccgggtcga aacggacctt
      361 cgggccggtc gcctgttgga ctggctcgag gctcgtcccg gcggcggaac gtcccagcaa
      421 ccgcccctgg ttcccgctct gctgtccgtc ggattgtccc tggggcggtc cctgggaacg
      481 gatcccgccg acgcggccgt gtgggtggtg ggccttttgt cgatcgtgtt cctttccatg
      541 ggggcctacc ggttcgcctt tcccgtgggc ggcgcggagg cggggctcgc cgcggccgcc
      601 ctggcctcga ccgttccgtt ttttttgact tggacgcgcc gaccggcgcc ggacgcggcc
      661 ctggcggcgg ccgtcgtgta cgcccacggg ttgtgggtgg ccaccgaagg ctttacccgg
      721 tttcgccgca cggtggcttt gggcgtcgcg ctgggggtgg gcgtcctcgt ccgttggacc
      781 tttttgttgt acgtcgcccc cgtgatcgcc gccgcggcct ggacgttctt tgtccgtcgt
      841 aaaaacaaac gtcggctcct tctcgggttt ctggcggcgg tggcggtggc cggtccctgg
      901 tatgtgctca acgccacggt cctgatcccg gcggcggtgg ccttttttcg gacccggtcc
      961 ggcgccggtc ctttccccgc ggggtggttt tggccggtgt tctccggatt tcccgcgctt
     1021 ctgtggccgc tcgcgggcgg ggcgggactt ctttgggctt ttttacgccg gcgacgtttt
     1081 ttcggccgtc cggtggcctg gttgtccacc ggtttggccg cggtcctttt ctgccccctg
     1141 aaggaggccc gggtcctcct gccggcggcc ttggcgctgc ccgtggccgc cgccgcgctg
     1201 ccctttcacg tgcccgccgt tttggcggcc ggggccctga ccgccaccgc ctggtccacc
     1261 tggggacccg attctgcccg ccgatcggtc cccgatccat cggcgctgga cgccattgtg
     1321 gaccgggtcc gggagcgttt gccgacgggc gattcaccgc cggtgctgac cctggtggcc
     1381 cgcgatgccc ttttaaacga accgtccttg gcgtggggct tcgaggaacg cggcctttcc
     1441 gatcggatcg aaatccaaac ccgactggat cggctgggac aatttgcgga attcctgctg
     1501 gtcaaaacag ctcccgcggg ggcctccggc gccggggaag cctgggagaa ggcccgggac
     1561 gaagtcctca accccgccgg ctggtttcgc cgggggttca acgaggccgg ccgatggccc
     1621 ctgccggacg gcacctcggc ggtgttgttg cagcggtcga cccaaagcgt cgcgatggaa
     1681 ggaccttcgg ggctggccgg catccttccc gggctcgggg acggcatctc gctggagggg
     1741 tttcaggggt ccctggagcc gagtcttcgt taccccggac aaagcatgtg ccgtttgtcg
     1801 accgatcggc tgacggtcgg cgcttggtcc atcggccggg tccaattgtt gttggacggt
     1861 ctggtcctcg cccgcgacga cgcgggccag ccgcgcctcc tcgatcttca tcgactggaa
     1921 ttggtgtccg cccggtggac cgcggccgac gcggagcgtc tgctcgcggc ccaagcgccg
     1981 gtcctgcggg acccccgcgt gcgctttttg gccgagaacc gggtggaggt gagcggacgt
     2041 tgttggggat tcccgctccg ccttcgggcc gccgtggcca ccgagtcggt ccccggaccg
     2101 gccctggaag cccgggtgga gcgtttttcc gtcggtcccg tgcccgttcc ggtcctggcc
     2161 ctcgggccct ggcggaaacg ccgcctggct ctctccccgg gcgggcggct tccggtccga
     2221 attcgttttc ccgcgctgcg caccgtcccc gccgatgaat caagcggagg ctatctggaa
     2281 tggggggccc catgacgtcg atggaattgt cggtgattgt cccggtgtat aacgaggaac
     2341 gggtgattga agtgttccac cgggccctgg tcgaggtgtt ggacggtctg gatttcccgt
     2401 gcgaaatcat ctatgtcaac gacggcagcc gggaccaaac gggggaccgc ttggacgccc
     2461 tgcgcgcgtc ggattcccgc gtccggctcc tggaactttc ccgaaatttc ggccaccaaa
     2521 gcgccctggc cgccgggctc tcccgggcct ccggccgggc ctgcgtcttc atggacgcgg
     2581 atttgcagga tccgcccgct ctgctgcccc gcttggtcga gcgttggcgg gccggggtcg
     2641 aagtcgttta cgccgttcgg cgcgcgcggc cgggcgattc cctgttcaag cgatggacgg
     2701 ctcgtttgtt ttaccggggg ttggggttga tcgcgggggt gtccctcccg cccgacgcgg
     2761 gggactttcg actgttggat cgaaaagtcg tggacgcctt aatcgccctc cccgagcggc
     2821 accgctattt tcgtggattg gtcagttggg tcggtttccg ccaggagggg gttccctttg
     2881 atcgtccggc ccgggccgcg ggggaaacga aattcactct ttggaaaatg atccgattcg
     2941 ccgtggacgg cgtgacgtcc ttttcccacg ttccgctccg gctcgtcacc ttggccgggt
     3001 tcgccgcttc ggcgggaagc gccgtgcttc tcgcctgggc tttttacgtc aagttcgtcg
     3061 cccatcgaac cgtccagggg tggacctcgc tgatcgcggt ggtgttgctg atgggcggcg
     3121 cccagttttt ggccctgggc attctgggcg aatacctggc ccgcgtgctg gacgaaacca
     3181 aaggccgccc gctgtttctc gttcgccgcg ccgcggggtt cggcgaggcc gccgccgatc
     3241 ggccgccgcg ccccgaatcc cgggcttgac cgggggcgga actcccccta cacttggggg
     3301 gaaccgatcg gggtcgcccc cggaacatcg atggaggagt tctcgtgccg cttcagctga
     3361 ccacgtcgtc gccccaggaa ttgtcgcccg aagacctcgc cccctttaaa gaaaccctgc
     3421 tcgcttacct ccgggcccgc aacgcgcaaa cccggcagca ggtcgattcg aacctccaca
     3481 tcactctcgg cgagaaaata ccgctctaca cgttc
//
LOCUS       DAOQLS010000152         3503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_14108_length_3503_cov_3.705046, whole genome shotgun sequence.
ACCESSION   DAOQLS010000152 DAOQLS010000000
VERSION     DAOQLS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14108_length_3503_cov_3.705046"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PK362_03890"
     CDS             <1..155
                     /locus_tag="PK362_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNC74014.1"
                     /translation="ILYGGSVKPDNIDQLMAEPDIDGGLVGGASLKAEDFLRIVRFNA
                     PAASRA"
     gene            152..601
                     /gene="rpiB"
                     /locus_tag="PK362_03895"
     CDS             152..601
                     /gene="rpiB"
                     /locus_tag="PK362_03895"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNC74015.1"
                     /translation="MKIALGSDHAGFAGKTALVRALRRAGHRVIDAGPADGNRVDYPD
                     FAAAVGRAVSAGRADRGVLVCGSGIGMAIAANKIRGVRAAVVWSEKTAGLAAAHNRAN
                     VLCLGGRLFRAPRLIRFVRIWLKTPFEAGRHVGRIDKIAALEKRARR"
     gene            598..1011
                     /locus_tag="PK362_03900"
     CDS             598..1011
                     /locus_tag="PK362_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74016.1"
                     /translation="MKIPWAVYPFLLWTGSLRAESAQDVVQQGLSEVRSGDVERGLSS
                     LRRAARLEPGNASVWITLGLTAQEARRYDDARDALERALQQAPRSETALYNLAMVYEK
                     LRDWSRARDAWTRFLALSPSADLKEIAQRHVDRLE"
     gene            1004..1555
                     /locus_tag="PK362_03905"
     CDS             1004..1555
                     /locus_tag="PK362_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74017.1"
                     /translation="MNRVCAIVLLVLAAGCLAPARVATLPGVDFSRIKTIAVLDPERE
                     DLRGITDEFVRQLMARGYTLRVGADGVPSPDALLQVNVGRMIPDKKYLIPIRSKDGQD
                     VLVMNPVTEISGRSLYPSATTAGLQDAQVVVSNATLSLSVRLLDPVSRNVWWTGSMTY
                     EGLDLDAAVEGSVTALLKRFPNR"
     gene            1862..2944
                     /locus_tag="PK362_03910"
     CDS             1862..2944
                     /locus_tag="PK362_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNC74018.1"
                     /translation="MAVDINKLFQTMIRSGISDIHFKAGTPPMVRMHGRLMSSGFNKM
                     APEHIEELANLLMSEEQRKAFQRESELDMSYHVPDLSRFRVNIYRQKGSVALSLRVVP
                     LQAKTFEELNLPTDTMKKICTNTRGMILVAGITGAGKTTTLNSMVDYINKNYAYNMIT
                     IEDPIEYYHANEKSSIAQREVGNDTPSFASAVKHLLRQDPDVVVLGEMRDAESIQAGI
                     TAAESGHLVLGTIHTMDASQTMGRLLESYHPAEQAGAKARIANVLKGIVAQRLLESAD
                     GRDRFPATEILVVTSLIRKLLMEDKFPEVNKAIEQGQYYGMHSFDQDIIRLFNERKIT
                     KEEALDASTNPDDLLVKMNTLRLGDM"
     gene            2989..>3503
                     /locus_tag="PK362_03915"
     CDS             2989..>3503
                     /locus_tag="PK362_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74019.1"
                     /translation="MSIERWEMLLSSRADRYILKIRGAKADVDKIVASNPRVCQDAQE
                     ISDAVFQWVIFVVDANQAERLAIQNQLIAMTSDPAVGPQASFDLSEMLTGLSDALENL
                     TNLTAEEQAFVAKKMKQVQERDAALPPVAPRPAAVPPVKPVAPLKGPAPVVAPKTPSS
                     PNGVPTPPAVHP"
BASE COUNT          696 a         1070 c         1071 g          666 t
ORIGIN      
        1 gaatcctcta cgggggttcg gtgaaacccg acaacatcga ccagttgatg gcggagccgg
       61 acattgacgg gggattggtc ggcggcgcca gcctcaaagc ggaagatttc cttcgaatcg
      121 ttcgcttcaa cgccccggcg gcctcccggg cgtgaaaatc gctctcgggt ccgatcacgc
      181 cgggttcgcc ggaaaaacgg cgctggtccg ggcgttgcgg cgggccggcc accgcgtgat
      241 cgacgcgggg cccgcggacg ggaaccgggt ggattacccc gatttcgccg ccgccgtggg
      301 ccgcgccgtc tccgccggcc gggcggaccg gggggtgttg gtctgcgggt cgggaatcgg
      361 gatggcgatc gccgccaata aaatccgcgg agtccgcgcc gccgtggtct ggtcggaaaa
      421 aaccgcgggc ctcgccgccg cccacaaccg ggcgaacgtc ctttgcctcg gggggcggct
      481 cttccgggcg ccacgattga ttcggttcgt ccgaatttgg ttgaaaactc ctttcgaagc
      541 ggggcggcac gtcggacgca tcgataaaat cgccgctctg gaaaagaggg cgcgccgatg
      601 aaaatcccct gggccgttta tccctttctc ctttggaccg gctcccttcg cgccgaaagc
      661 gcccaggacg tggtccaaca ggggttgtcc gaggtccgct cgggggacgt ggagcgtggg
      721 ttgtcctccc tgcgccgcgc ggcccggttg gaacccggaa acgcctccgt ttggatcacg
      781 ttgggcctca ccgcccagga ggcccgccgg tacgacgacg cccgggacgc cttggaacgg
      841 gccctccagc aggctccccg atccgaaacg gccctctaca acctggcgat ggtctacgaa
      901 aaattgcggg attggtcccg ggcccgggac gcttggactc gatttttggc tctgtcgccc
      961 tccgcggacc tgaaggaaat cgcccagcgg cacgtggacc gacttgaata gagtttgcgc
     1021 gatcgttctg ttggtccttg cggcgggctg tttggccccc gcgcgtgtcg ccacgttgcc
     1081 cggggtggat ttctcccgaa tcaagaccat cgccgtccta gacccggaac gcgaagattt
     1141 gcgcgggatt acggatgaat ttgttcgcca attaatggcc cgcggttaca cccttcgggt
     1201 gggcgccgat ggcgttccct cccccgacgc gttgcttcag gtcaatgtcg ggcggatgat
     1261 tcccgacaaa aaatatctga ttcccatccg atccaaggac ggccaggacg tcctcgtgat
     1321 gaatcccgtc accgagattt cgggccgaag cctttacccc tcggcgacga cggccggtct
     1381 tcaggacgcc caggtcgttg tttccaacgc caccctctcc ctttccgtcc gcctgttgga
     1441 ccccgtcagc cggaatgttt ggtggaccgg ttcgatgacg tacgagggcc tggatttgga
     1501 cgcggcggtg gaagggtccg taacggcttt attaaagagg tttccgaacc gttaattgag
     1561 gggtttaacg cgcttcgagg ggttgaaacg gcggatttca tagtgcatac tgattgggcc
     1621 cactgtcctg tgtttgcaac gggttggcca gcggtttttg gcccgttgaa atccgcggcg
     1681 gagggaacgg ccggggaggg aaaaagcgaa tcacgccgct gaatcggcgg ttgtgggaaa
     1741 cccggggccg tgataaaatg aacgtttcgg aattgtaaca ggagcttcgt gcgaacatcg
     1801 ggggtcgggg aacgattccc ttgcgcgcgg cgaacgacca atcgcatcaa ggaggtcttc
     1861 ggtggccgtt gacatcaata aactctttca aaccatgatc cgaagcggca tttcggacat
     1921 tcacttcaag gcgggcacgc caccgatggt tcgtatgcac ggccgactga tgtcgtccgg
     1981 gttcaacaaa atggcccccg aacatatcga ggaattggcc aacctcctga tgagcgagga
     2041 acaacgcaaa gcctttcaac gggaaagcga attggacatg tcctaccacg tgcccgatct
     2101 ctctcgtttc cgcgtcaata tttaccgaca aaagggatcc gtggctttgt ccctgcgcgt
     2161 cgttcccctc caggccaaga cctttgaaga gctgaacctg ccgaccgaca ccatgaagaa
     2221 gatctgcacg aacacccggg gcatgatcct ggtggcgggc atcaccgggg cggggaaaac
     2281 gacgacgttg aactccatgg tggactacat caacaagaat tacgcctaca acatgatcac
     2341 catcgaagac cccatcgagt attaccacgc caacgagaaa tcgtccatcg cgcagcgcga
     2401 agtcggcaac gacacgccgt ccttcgcctc ggccgtcaaa catttgctcc gccaggaccc
     2461 ggacgtggtg gtcctggggg aaatgcggga cgccgaatcg atccaggccg gcatcacggc
     2521 cgcggaatcc ggccacctcg ttttgggaac gattcacacc atggacgcct cccaaaccat
     2581 ggggcgcctg ctggaatcct atcacccggc cgaacaggcc ggcgccaagg cgcgcatcgc
     2641 caacgtttta aagggcatcg tcgcccagcg gctgttggaa tcggccgacg ggcgggatcg
     2701 tttccccgcg acggaaatcc tcgtggtgac gtccttgatt cgaaaactct tgatggaaga
     2761 taaatttccc gaagtgaaca aggccatcga acagggccag tattacggaa tgcattcctt
     2821 cgaccaagac atcatccgtt tgttcaacga gcggaaaatc acgaaagaag aagccctgga
     2881 cgcctccacg aacccggacg atttgctggt gaaaatgaac accctccgcc tgggggacat
     2941 gtaggcccac gcgccccgtc catggcgatg gctgaggaga gtcgttgaat gtcgattgag
     3001 cgttgggaaa tgcttttgtc ctcccgggcg gaccggtata tcctgaaaat ccgcggcgcc
     3061 aaagccgacg tggataaaat cgtcgcctcg aacccccgcg tgtgccagga cgcccaggaa
     3121 atttccgacg ccgtttttca gtgggtgatt ttcgtcgtcg acgccaatca ggccgaacgg
     3181 ttggccattc aaaaccagtt gatcgccatg acctcggacc cggccgtggg tccccaagcg
     3241 tcgttcgatt taagcgaaat gttgacgggg ttgagcgacg ccctcgaaaa tttgaccaat
     3301 ttgaccgcgg aagaacaggc ctttgtcgct aaaaaaatga agcaggtcca agagcgggac
     3361 gccgcccttc cgcccgttgc gccgcgcccg gcggcggtgc cgccggtgaa gccggtggcg
     3421 cccctgaagg ggcccgcccc ggtggtcgct cccaagacac cgtcttcgcc caacggcgtt
     3481 ccgacccctc ccgccgttca ccc
//
LOCUS       DAOQLS010000153         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_14193_length_3492_cov_5.459703, whole genome shotgun sequence.
ACCESSION   DAOQLS010000153 DAOQLS010000000
VERSION     DAOQLS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14193_length_3492_cov_5.459703"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PK362_03920"
     CDS             <1..1291
                     /locus_tag="PK362_03920"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNC74020.1"
                     /translation="IIYFQSAGSGYSAVVSTVTMKGAKTRELKCTLGGGQFCGVAEEL
                     GGMSLRGNESIRFYAGSNFGGSSVKMEVKDVASVTFSTTVTGLPAPPANFSTYTFSAA
                     AIQAAQPAIDLSALKEIVFVFDTAGAHDLYFDDLSIVPPAATLADLSVVEDFGSDLAE
                     GNFVTAAPTQATVGLSYVDDPTSLGAGADNRVAKLDYSFAVDPSTPFAVAERTLGINL
                     LAEPGLRFRFKGTGGNENLEVKITDGDGTIYVKKLTEVTDTGGVWKTATLKSDQFSFS
                     TLGSDARLDLKNITKVDFAVSLGKAAAGTLALDNLESLPATDFQKTSIGRVLTQVTTP
                     NNPFSPNGDGIKDSFRLTYTLSQTAHVVLRFYALHGALIKTFDVGDQTAGDHAIDWDG
                     TADDGRRVGNGLCLFSLEADGAIDGKDTFKEIAGVLR"
     gene            1288..2151
                     /locus_tag="PK362_03925"
     CDS             1288..2151
                     /locus_tag="PK362_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74021.1"
                     /translation="MNRRWSFAFLLLAAPALRAAFEPLGYGAAAKGMGGAYVASAEDG
                     TAAYWNPAGLALVRQPQLTASMEDLYGLGLLRYAAVGYTHPRLGGGTLSAHLLHLDTT
                     GEADFYKYAESTYLVAYGKSLCQDCLSVGAGLRYYAAQGGGARGSGVGLDLGLLWRPF
                     DDRLRLSAAWQDANQPSIRWDTGAEDLQPYTVRTGALFRLTTTTDLAAEWDKRRFEKA
                     QWRFGGAQRLMKNLVTFRGGLHRGGDQEQWGFSVGGGFHFRTFDVDYAWDSQEALGNT
                     QTMSLGLRFGQ"
     gene            2148..3434
                     /locus_tag="PK362_03930"
     CDS             2148..3434
                     /locus_tag="PK362_03930"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNC74022.1"
                     /translation="MKILRPVLVGAVLVGALSMVSTGLRAAGPVLLLDNFDEDKTENA
                     LGGATGAWMDPEDKSQGCTASRVQLDRLGSLGRSLRLDFDIESQRENIRVPTNFSVAS
                     PAVALNQAFNGYYSVFPAQDLRPYNYLIFWAKGDATAGYTRSFKIEIKDGLSSGYKGF
                     VMDGLGTEWRRFVVPLRDFADIKDWGAVKEFVIVFAADAVTRKNGTVYIDDVYFAQNP
                     DNNLTLPEREMTARHAEKPVTLDGKPSEWPRRDFIDLSPSEFVESGARGLRDAGARFA
                     VQWDEQWLYLTVDLEDNEIQNGEEGESLWKGDVVELYVNPTGPVFTWGDPSAFQLGFS
                     PVSASGVPGRWAWFQRRAPKENEARYAWDKKKHTAEIAVAWSFLGVTPGVNRDLGFSI
                     AFHDKDSADGTPEAKLQWSFQSLGGKKARLGKLVLK"
BASE COUNT          648 a         1091 c         1077 g          676 t
ORIGIN      
        1 catcatttat ttccagagcg cggggtcggg atactccgcg gtcgtttcga cggtcacgat
       61 gaaaggggcc aaaacccgtg aactcaagtg caccttgggc ggcgggcaat tctgcggcgt
      121 ggccgaggag ctgggcggaa tgtccctccg cggcaacgaa agcattcggt tttacgcggg
      181 ttcgaatttc ggcggttcct ccgttaaaat ggaagtgaaa gacgtggcca gcgtcacttt
      241 cagcaccacg gtcacggggc tcccggcgcc cccggccaac ttttcgacgt acactttttc
      301 cgcggccgcc atccaagcgg cccagcccgc catcgacctg agcgctctca aggaaattgt
      361 ttttgtcttt gacaccgccg gcgcccacga cctttatttc gacgacctgt ccatcgttcc
      421 gcccgcggcg actctggccg acctgtccgt cgtggaggat tttgggtccg atctggccga
      481 agggaacttc gtcacggcgg cacccaccca ggccaccgtg ggactgtcct acgtggacga
      541 tccgacgtcc ttgggcgccg gggccgacaa ccgggtggcc aagctggatt acagctttgc
      601 cgtggaccct tcaacgccct tcgccgtggc ggagcggacg ttgggcatca acctcctggc
      661 cgagcccggt cttcgcttcc ggttcaaagg gaccggggga aacgaaaatt tagaggtcaa
      721 aatcaccgac ggcgacggaa cgatctacgt aaagaaattg accgaggtga cggacaccgg
      781 gggggtctgg aaaacagcca ccctcaaaag cgaccagttt tccttcagca cgctcggcag
      841 cgacgcgcgg ttggatttaa agaacattac gaaggtggac ttcgcggtgt ctctgggcaa
      901 agcggccgcg gggaccttgg ccttggacaa tctggaatcc ctgccggcca ccgattttca
      961 aaaaacgtcc atcggacgcg tgttgaccca ggtgaccacc cccaacaatc ccttttcccc
     1021 caacggggac ggcattaagg actccttccg tttaacctac accctcagcc aaacggccca
     1081 cgtggttctc cggttttacg ccctgcacgg ggctttgatc aaaaccttcg acgtcggcga
     1141 tcaaacggcg ggggaccacg ccatcgattg ggacggcacc gcggacgacg ggcggcgggt
     1201 cggcaacggc ctgtgcctgt tctccttgga ggccgacgga gctatcgacg ggaaagacac
     1261 gtttaaagaa atcgccgggg tgctccgatg aaccgccgct ggtcctttgc tttccttttg
     1321 ttggccgctc ccgccctgcg ggcggcgttc gagcccctgg ggtacggcgc cgcggccaag
     1381 ggcatggggg gcgcctacgt ggcctccgcc gaagacggca ccgccgccta ttggaacccg
     1441 gccggcctgg ccctggttcg tcagccgcaa ttgaccgctt ccatggaaga cttgtacgga
     1501 ttgggcctcc ttcgctacgc ggccgtgggt tatacccatc cccggctcgg cgggggaacg
     1561 ctgtcggccc atttgctcca tctcgacacg acgggcgaag ccgattttta taaatacgcc
     1621 gagagcactt acctggtggc ctacggcaaa tccctctgcc aggactgcct gagcgtgggg
     1681 gcggggctcc gttactacgc cgcccagggc ggcggcgccc gggggtcggg cgtcggcctc
     1741 gacctgggtc ttttgtggcg tcccttcgac gaccgcctgc gcctgtcggc cgcttggcag
     1801 gacgccaatc agccctccat tcgctgggac acgggcgccg aggatctcca gccctacacc
     1861 gtccggacgg gcgccttgtt ccgtttgacc accacgacgg atttggcggc ggaatgggac
     1921 aagcgacgat ttgagaaggc ccaatggcgg ttcggcggcg cccagcggtt gatgaaaaat
     1981 ctggtcacct tccggggcgg cctccaccgc gggggcgacc aggagcaatg gggcttttcc
     2041 gtgggcggcg gtttccactt ccggaccttt gatgtggatt acgcctggga cagccaggaa
     2101 gctctcggca acacccaaac catgagtctc ggattgaggt tcggccaatg aaaatccttc
     2161 gtcccgttct cgtcggcgcg gtcctggtcg gagccctctc gatggtttcc acgggccttc
     2221 gcgccgcggg ccccgtcctc ctgctcgaca atttcgatga agacaagacc gaaaacgccc
     2281 tgggcggcgc caccggcgcc tggatggacc ccgaagacaa aagccagggg tgcaccgcct
     2341 cgcgcgtcca gctggaccgg ctgggctccc tcgggcgaag cctgcgactt gatttcgaca
     2401 tagaaagcca acgggaaaat attcgggtcc ccacgaattt ttccgtggcc agcccggcgg
     2461 tcgctttgaa ccaggccttc aacggttatt actcggtgtt cccggcccag gacctgcgcc
     2521 cctacaacta cttgattttc tgggccaagg gcgacgccac cgccggctac acgcgttcct
     2581 ttaagattga aatcaaagac ggtctcagct ccggctacaa aggatttgtg atggacggat
     2641 tgggcacgga atggcgccgg tttgtcgtcc cccttcggga tttcgccgac atcaaggact
     2701 ggggcgcggt gaaggaattc gtgatcgttt tcgccgccga cgccgtgacg cgcaaaaacg
     2761 gcactgtcta catcgacgac gtgtatttcg cccaaaaccc cgacaacaat ttgaccctcc
     2821 ccgaacggga aatgacggcc cgtcacgccg agaaaccggt aaccctggac ggcaagccct
     2881 ccgaatggcc ccgccgggat ttcatcgatt tgtccccttc cgaattcgtc gaatccgggg
     2941 cccgggggct tcgggacgcc ggggcccgct tcgccgtcca gtgggacgag caatggcttt
     3001 atttgaccgt tgatctggaa gacaacgaga tccaaaacgg cgaagagggc gaatccctgt
     3061 ggaaaggcga cgtggtggag ctttacgtca accccacggg tccggttttc acttgggggg
     3121 atccgtcggc gttccagctt ggtttctccc cggtgtcggc ttcgggcgtg ccgggccggt
     3181 gggcgtggtt tcaacgccgg gcccccaagg aaaacgaagc ccgttacgcc tgggacaaaa
     3241 aaaagcacac agcggaaatc gccgtggcct ggtcgttttt gggggtcacc ccgggcgtca
     3301 atcgggacct gggtttttcc atcgcttttc acgacaagga ttccgcggac ggaacgccgg
     3361 aagccaagct ccagtggagt ttccagtccc tgggcggtaa aaaagcgcga cttggaaagt
     3421 tggtgttgaa atgaagccca ctcgacgatg gaattccgcc ctggccgcct ttctctggtg
     3481 gggggccaat gt
//
LOCUS       DAOQLS010000154         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_14527_length_3444_cov_3.941871, whole genome shotgun sequence.
ACCESSION   DAOQLS010000154 DAOQLS010000000
VERSION     DAOQLS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14527_length_3444_cov_3.941871"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..2213)
                     /locus_tag="PK362_03935"
     CDS             complement(192..2213)
                     /locus_tag="PK362_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNC74023.1"
                     /translation="MMPALFSGVSGLRNHQFRMNVIGNNLANVNTVGFKYSRVSFSDL
                     VYQTIRDASAPQSVGGSNPVQMGLGSLVHSVDVVNTQGNLESTGRNTDLSIQGEGFFV
                     LNDGAQNKYTRAGIMVMGLNGSLVNPADGLNFMGWNAVNGVVDNSGPLTTINIPVGDV
                     LPAQQTTHLTFNGNLNATGNVAQGTIADSHGLLAAAGGGTLLTGMKNSAGGALGLNAG
                     DVISISGSVGGTLIGGATLNVNAGTTLTDLANALQAALRSVPDGDLTETVSVQSDGSL
                     RVTSDGSNNIANLQMTVAGNTLFNNALRFPSSIAGGGSTGDSDTLRRSAQSTDTLVNL
                     YDSTGTALGLQVGDDVTLVSASAKGAILSDVALLADITGATTYADYRDALRAALFTAA
                     PAVGEDVVIQPSGALEITGAAGASEAITGIVIGAGPNPGDDQRSAFSSSQSFAESQKA
                     LDATSFRTAPQIFDSLGNALNVDFRFTKTANNSWQWDATYGGTAVGSGVLNFGTDGQL
                     TTPTGSVSIPLTNGAASPLAVGVDFTGTTQFAGTSSVVLKSQDGYASSTLNGFSIGQS
                     GDVTGVFSNGKNVTLGQLAIASFRNPSGLLREGRNLYVESPNSGVASAGVAGINGRGL
                     IVSGTLEMSNVDVAREFTDMIVTQRGFQANSRVITASDQLLEELVNLKR"
     gene            complement(2288..2635)
                     /locus_tag="PK362_03940"
     CDS             complement(2288..2635)
                     /locus_tag="PK362_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HNC74024.1"
                     /translation="MIPGVGPVAPGAIERPPSAGPRDGFDAILKREALRFSKHAEDRL
                     VRDGIALNDEDRERLQSGMDRAAAKGAKEALLLLDDKAFIANVREKIVITSMSRDRLK
                     ENIFTRIDSAVVL"
     gene            complement(2632..3039)
                     /locus_tag="PK362_03945"
     CDS             complement(2632..3039)
                     /locus_tag="PK362_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNC74025.1"
                     /translation="MTTTPGVDSSDPTQYTANNGGLGNIGKSDFLKLLTAQLQNQDPM
                     KPSSQEEMVATLAQFTALEQSAHLVEQMTTLSKSNAWNQGVSLMGRTVQALDDAGASV
                     SGTVSGLRWDGDALRLRVGTADVALANLVQVLS"
     gene            complement(3062..>3444)
                     /locus_tag="PK362_03950"
     CDS             complement(3062..>3444)
                     /locus_tag="PK362_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNC74026.1"
                     /translation="DWHWRGGKGDVTIALHPRDLGRVSVRMETVNDVVSVRFEVDNEA
                     ARAGLAHRSDELTQSLRDLGWRVDNVHVSLAGAGTDAFSRNGQSSSDGSRSPFRWPSA
                     ARGTIPALPEARGGPSPHLVDLVA"
BASE COUNT          601 a         1071 c         1127 g          645 t
ORIGIN      
        1 ccccgccgtg ggaaggcggc ggggccgcga ccgcggcggc gggagcggtc ccgatcgggg
       61 ccagcgcccg ggccacgacg acccgcgacg tggcgatgca aagaaccccg gacacggtca
      121 ggagcaggac ttcccccacc cctttgacaa tcgtttcttt gcttaccagc gccacgacga
      181 ccctcccggt cctaccgttt taaattgacc aactcttcca gaagctgatc ggacgcggtg
      241 atcacccggg aattggcttg aaacccgcgt tgcgtcacga tcatgtccgt aaactcccgg
      301 gccacgtcga cgttggacat ttccaacgtt cccgaaacga tgagccctcg cccgttgatg
      361 ccggccacac cggccgacgc caccccggag ttcggggatt ccacgtagag gttccggcct
      421 tcccgaagca accccgaagg gttgcggaag gaggcgatgg ccaattgacc gagcgtcacg
      481 tttttcccgt tggagaacac cccggtgacg tcgccggatt ggccgatgga aaacccgttg
      541 agcgtggaac tggcgtagcc gtcctgactt ttcagaacga cggaggacgt gcccgcgaac
      601 tgggtcgttc cggtgaagtc cacgccgacc gccaggggcg aggcggcgcc gttggtgagc
      661 ggaatcgaca cgctgccggt gggcgtcgtc agctgcccgt cggtgccgaa attcaacacc
      721 ccgctgccga cggcggtgcc gccgtacgtc gcgtcccatt gccaggagtt gttcgcggtt
      781 ttcgtgaatc ggaaatcgac gttcagggcg ttgcccaggg aatcaaagat ttggggagcc
      841 gtccggaagc tggtggcgtc caacgctttc tgggattccg cgaaggattg cgacgaggaa
      901 aaggccgacc gctggtcgtc cccgggattc ggaccggcgc cgatcacaat gccggtgatg
      961 gcctccgaag cgcccgcggc gcccgtgatt tccaaagcgc cgctcggttg gatgaccacg
     1021 tcctcgccga cggccggagc ggccgtgaag agggcggccc ggagggcgtc gcggtagtcc
     1081 gcgtaggtgg tggctccggt gatgtcggcc aacagggcca catcggacaa aatggcgcct
     1141 ttggcgctgg ccgacaccag ggtcacgtcg tccccgactt gaaggcccag ggcggttccg
     1201 gtggaatcgt aaaggttcac gagcgtgtcg gtgctttgag ccgaccgccg cagggtgtcc
     1261 gaatcgcccg tggatccgcc gcccgcgatg ctggagggga agcgaagcgc gttgttgaac
     1321 aacgtgttgc ccgccacggt catttgcaga ttggcgatgt tgttggatcc gtcggaggtc
     1381 acccgcaggg atccgtcgct ttgaacggac accgtttcgg tcaagtcgcc gtcgggcacg
     1441 gaccggaggg cggcttgaag ggcgttcgcc aagtcggtca gggtggtccc cgcgttgacg
     1501 ttcaaggtcg cgccgccgat caacgttccg ccgacgcttc ccgaaatgga aatcacatcg
     1561 ccggcgttca gtcccagggc gccgcccgcg ctgtttttca tccccgtcaa caaggtgccc
     1621 ccgccggccg cggccaggag cccgtggctg tcggcgatcg tcccctgggc cacgttgccc
     1681 gtggcgttca ggtttccgtt gaaggtcaaa tgcgtggttt gctgggccgg gagcacgtcg
     1741 ccgaccggaa tgttgatcgt ggtcaatgga cccgaattgt cgaccacgcc gttgacggcg
     1801 ttccacccca tgaaattgag gccgtcggcc ggattgacca acgacccgtt cagccccatg
     1861 accatgatgc ccgcccgggt gtatttgttc tgggcgccgt cgttcaaaac gaagaaccct
     1921 tctccctgaa tggaaagatc ggtgttccgc ccggtggatt ccagatttcc ctgggtgttc
     1981 accacgtcga cgctgtgcac caaggaaccg aggcccatct ggacggggtt ggaaccgccc
     2041 accgattgcg gcgcggaggc gtcccgaatc gtctgataaa ccaaatcgct aaaactgacg
     2101 cggctgtatt tgaagcccac ggtgttcacg ttcgccaagt tgttgccgat cacgttcatg
     2161 cggaactggt ggtttcgaag acctgatact cctgagaaga gagccggcat catcggggga
     2221 ttcctcctga tttagaatgc gcttcgcggt gtggcatttt taccaagttg caaaagccgt
     2281 gccgttgtca aaggaccacg gcgctgtcga ttcgggtgaa aatattttcc ttgagccgat
     2341 cccgactcat ggaggtgatc acgatttttt cgcggacgtt ggcgatgaag gccttatcgt
     2401 ccaacaggag caacgcttcc ttggccccct tggcggcggc gcggtccatc cccgactgga
     2461 ggcgttcgcg gtcctcgtcg ttcagcgcga tgccgtcccg caccagtcgg tcttccgcgt
     2521 gtttggaaaa acgcagggct tcccgcttga ggatcgcgtc gaaaccgtcc cgcggcccgg
     2581 cggacggcgg acgctcaatg gctcccgggg cgaccgggcc gacccccgga atcacgagag
     2641 cacctgcacc aggttggcga gcgccacgtc ggcggttccc acgcgcaaac gaagggcgtc
     2701 cccgtcccat cgcaggccgg agaccgtccc ggacacggag gccccggcgt cgtccagggc
     2761 ttgaacggtc cggcccatca gggacacccc ctggttccag gcgtttgatt tggaaagggt
     2821 cgtcatttgc tcgaccagat gggccgattg ttccagggcc gtaaattggg ccagcgtggc
     2881 gaccatttcc tcctgggacg acggtttcat gggatcctgg ttctgaagct gggcggtgag
     2941 cagtttgagg aagtccgatt tgccgatgtt gccgagcccg ccgttgttgg cggtgtactg
     3001 ggttgggtcc gaagagtcga cgccgggggt cgtggtcatg aggtctcctt tttaagatgc
     3061 gctacgcgac caaatcgacg aggtgggggg agggccctcc acgggcttcg ggcagcgccg
     3121 ggatcgttcc gcgggcggcg gagggccagc gaaagggcga gcgggacccg tccgaagagg
     3181 attggccgtt ccgggagaaa gcgtcggtgc ccgctccggc cagggacacg tgcacgttgt
     3241 ccacgcgcca tccgaggtcc cgcagggatt gcgttaattc gtccgaacgg tgggccaacc
     3301 cggcccgggc cgcttcgttg tccacctcaa atcgaaccga cacaacgtcg ttgaccgttt
     3361 ccattcggac cgaaacgcgg cccaagtccc ggggatgaag agcgatcgtt acgtcgcctt
     3421 tccccccccg ccaatgccaa tcca
//
LOCUS       DAOQLS010000155         3440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_14557_length_3440_cov_4.068833, whole genome shotgun sequence.
ACCESSION   DAOQLS010000155 DAOQLS010000000
VERSION     DAOQLS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14557_length_3440_cov_4.068833"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2586)
                     /locus_tag="PK362_03955"
     CDS             complement(<1..2586)
                     /locus_tag="PK362_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNC74027.1"
                     /translation="MDKRFLKEDGWAAFTGNELLLKGALEGGIALLTGYPGSPVSEVF
                     DAVGRAAPLLLENGVVAQMANNEALAAARLNGARLANLRAMAVMKSVGMHVAADGLAI
                     GNLSEPRQPAGGALVVVGDDPWNETTQINSDSRFLSLHLHMPVLEPATFQEIKDWIKD
                     AFELSGETDLYFTYVLTTNQADGGGTVRCRPNLFPSVNHLQRTLLTAGQLPVDRTVMI
                     PPHTSLREATLAARYQKLLAAVRTRGLNRVEGPAGAAPLGFVSAGLSYGYLVQALDEM
                     GLAGHFPVLKFGMSFPLDETLLRDFAARVEHLVVVEEKRDFLETQVMRALQRAHQDGR
                     LARPPAVWGKKFPDELPGFPVERGLNVAIVMDRLAPLLEMKMAGPAAPRTGAVARARQ
                     TADYKISIPIRTPTFCPGCPHRDSSTVTKALKADFQDAAFMKQNHKRDPVEVIFHGES
                     GCHSMLQFEPNIGLMQDYSGMGLGGGTGSGMDPFIKNKQVVFLGDSTFFHSGMLAVSD
                     SIKNGQDITYIILENKTTAMTGHQPTPGSAENLMSEKTFAQDIEQVIRGMARDSIPIF
                     RVNPAYRESYRALLEQVILKSGVKVVIADKECGITYHRRVRKERKKIVRALGFMPEEK
                     VINITPEVCEYCLECTNATGCPGLGIEETLHGPKIVTDKSLCIADGACAKGKVCPSFE
                     EITIRRRRAPGPRPWESAPLPPVTPKPLGEGWYCYTAGVGGMGSGVITGILVQAGMLQ
                     GYHVLFADKKGLAIRNGGVYGHVIYSPDERVKAPLVPYGRADVLLGIDLLESVRGLDP
                     KVNLRVASPDRTVAVVNTHKMPTVKTLLGKDDFSVLELEAALRTHTRADQYLGLDFSN
                     ISEAFF"
     gene            complement(2614..>3440)
                     /locus_tag="PK362_03960"
     CDS             complement(2614..>3440)
                     /locus_tag="PK362_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNC74028.1"
                     /translation="TLADVVAYRARVAEETLARLDQLAKLKKKYGPTLADVVAYRARV
                     AEELDRLENLENKNRDMAERLTSAEKQVAARSEKLSAARRAAAKKLETAINKEFREVG
                     LPHAALDIESAAEPGRYTSAGVDRVQFWFTPNPGEGRRPLADVASGGELSRVMLAIKS
                     VLAKTDAVPVLVFDEIDAGVGGALGAVLGKKLAKLGASHQVLCVTHLATIAACGDRHF
                     VVEKEIQKTRTRTTVRAVTDSDRVQEIARLFGGTGKAAEGDIGLRHARELLDSSRR"
BASE COUNT          562 a         1089 c         1151 g          638 t
ORIGIN      
        1 gaagaaggcc tcggaaatgt ttgaaaagtc gaggcccaaa tactggtcgg ctcgggtgtg
       61 ggtccgaagg gcggcttcca attcaaggac ggaaaaatcg tccttgccca ggagcgtttt
      121 gaccgtcggc atcttgtggg tgttcaccac ggccacggtg cggtccgggc tggccacccg
      181 caggttgacc ttgggatcca ggccccggac ggattccaac aggtcgatgc cgagcaggac
      241 gtccgcccgc ccgtaaggga cgagcggcgc tttgacccgt tcgtcgggtg agtagatgac
      301 gtgcccgtag acgccaccgt ttcgaatggc gaggcctttc ttatcggcga acaaaacgtg
      361 gtacccctgg agcatcccgg cctggaccaa tatcccggtg atgacgcccg accccatgcc
      421 gcccacgccg gcggtgtagc agtaccagcc ttcgccgagg ggcttggggg tcaccggcgg
      481 caagggcgcg gattcccagg ggcggggccc cggggcccgg cggcggcgga tggtgatttc
      541 ctcgaaggac gggcacactt tccccttggc gcaggcgccg tcggcgatgc agagggattt
      601 gtccgtgacg attttggggc cgtgcagggt ttcctcgatg ccgaggcccg ggcacccggt
      661 ggcgttggtg cattccaggc agtattcgca aacttcggga gtgatgttga tgaccttttc
      721 ttccggcatg aaccccaggg cgcggacgat ttttttgcgc tccttgcgga cccgccggtg
      781 gtaggtgatc ccgcattcct tgtcggcgat gaccaccttg acgccggatt tcaaaatgac
      841 ctgctccaag agcgcccggt aggattcgcg gtaggccggg ttcacccgga aaatggggat
      901 gctgtcccgg gccatgcccc gaatgacctg ttcgatgtcc tgggcgaagg tcttttcgga
      961 cattaaattc tcggcgctcc cgggcgtggg ctggtggccc gtcatggccg tggtcttgtt
     1021 ttccaggatg atgtaggtga tgtcctgccc gttcttgatg gaatccgaca ccgcgagcat
     1081 gccggagtgg aagaaggtcg agtccccgag aaacaccacc tgcttgtttt tgatgaaggg
     1141 gtccatgccc gagcccgttc cgccgcccaa ccccatgccg gaataatcct gcatgaggcc
     1201 gatgttgggc tcgaactgga gcatggagtg gcagccggac tcgccgtgga atatcacttc
     1261 gacggggtcg cgcttgtggt tttgcttcat gaacgccgcg tcctggaaat cggccttcag
     1321 ggccttggtc accgtcgagg agtcccggtg ggggcacccg ggacagaagg tcggcgttcg
     1381 gatcgggatg gaaatcttgt agtccgcggt ttgacgcgcc cgggccacgg ccccggtgcg
     1441 gggggcggcg ggtcccgcca ttttcatttc caggaggggc gccaggcggt ccatcacgat
     1501 ggcgacgttc aacccccgct ccacggggaa ccccgggagt tcgtcgggaa acttcttccc
     1561 ccagacggcg ggcgggcgcg ccaggcgccc gtcctggtgg gcgcgctgga gggcgcgcat
     1621 cacctgggtt tccaggaaat cccgtttttc ctccacgacc acgaggtgtt ccacccgggc
     1681 ggcgaaatcc cgcaacaggg tttcgtccaa ggggaaggac atgccgaatt tgagaacggg
     1741 gaaatgaccg gccaggccca tttcgtccag ggcctgcacg aggtagccgt aggacaggcc
     1801 ggccgacacg aaccccaggg gcgcggcgcc cgcggggccc tccacgcggt tcaagccccg
     1861 ggtccggacg gccgccagca gcttttggta acgggccgcc agcgtggcct cccgaaggga
     1921 ggtgtggggc gggatcatga cggtgcggtc cacgggcagc tgccccgcgg tcaaaagggt
     1981 cctttgaaga tgattcaccg acggaaacaa attgggccgg caacgcacgg tgccgccgcc
     2041 gtcggcctgg ttcgtcgtca gaacgtaggt gaagtaaagg tcggtctccc cggacagttc
     2101 gaaggcgtct ttgatccagt ctttgatttc ctggaaggtg gcgggttcga gcacgggcat
     2161 gtgcaggtgg aggctcagga accgggaatc ggagttgatc tgggtggttt cgttccaggg
     2221 atcgtcgccc acgacgacca gggcgccgcc ggcgggctgc cggggctccg agaggttgcc
     2281 gatggccagg ccgtcggcgg ccacgtgcat gccgacggat ttcatgaccg ccatggcccg
     2341 caggttggcg agccgggcgc cgttcagccg cgcggcggcc agggcctcgt tgttggccat
     2401 ctgggccacg acgccgtttt ccaggagaag aggcgccgcg cgtccgaccg cgtcgaacac
     2461 ctccgacacc ggggaccccg ggtagccggt caaaagggcg atgccgcctt ccagggcgcc
     2521 cttcagcaga agttcgttgc ccgtgaaggc cgcccaaccg tcttctttta gaaatcgttt
     2581 gtccacaaat cctccgataa aaaaccgcgg cgcctaacgg cgcgaagagt ccaacaattc
     2641 ccgggcgtgc cgcaggccga tgtcgccttc ggcggccttg cccgtgccgc cgaagaggcg
     2701 ggcgatttcc tgcacccggt ccgaatccgt caccgcgcgg accgtggtcc gcgtccgggt
     2761 tttttgaatt tctttttcca ccacgaaatg ccggtcgccg caggcggcga tggtggccaa
     2821 atgcgtcacg cagagcacct ggtgggaggc gcccagcttc gccaattttt tccccaagac
     2881 cgcgcccagg gcgccgccca cgccggcgtc gatttcgtcg aacaccaaca ccgggacggc
     2941 gtcggttttg gccaaaacgc ttttgatcgc gagcatcacc cgggacaatt cccccccgga
     3001 cgccacgtcg gccagggggc gccgtccttc gccggggttc ggcgtgaacc aaaactggac
     3061 ccggtccacc cccgcgctcg tgtagcggcc gggctcggcc gcggactcga tgtccagggc
     3121 ggcgtggggc aaacccacct cccggaactc cttgttgatc gcggtctcga gcttcttggc
     3181 cgccgcccgc cgggccgcgg acagtttttc cgaccgggcg gccacctgct tttccgccga
     3241 ggtcagtcgc tcggccatgt cccggttctt gttttccaaa ttctccagac ggtccaattc
     3301 ctcggccacc cgcgcccggt aggccaccac atcggccagg gtggggccgt atttcttctt
     3361 taatttcgcc agttggtcca gacgggccag ggtctcctcg gccacccgcg cccggtaggc
     3421 caccacatcg gccaaggtgg
//
LOCUS       DAOQLS010000156         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_14814_length_3408_cov_4.213242, whole genome shotgun sequence.
ACCESSION   DAOQLS010000156 DAOQLS010000000
VERSION     DAOQLS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14814_length_3408_cov_4.213242"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK362_03965"
     CDS             <1..203
                     /locus_tag="PK362_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNC74029.1"
                     /translation="MTCGKPWSTRGSSGASTAASTGYPRTLEELGRIFSVTRERVRQI
                     EAKAIRKLRHPSRSRFLREYVE"
     gene            220..708
                     /locus_tag="PK362_03970"
     CDS             220..708
                     /locus_tag="PK362_03970"
                     /inference="COORDINATES: protein motif:HMM:NF020366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HNC74030.1"
                     /translation="MAVLVAGAAPVRAELGERHVERAGGFSYCPPEGWSVREFPGFKY
                     KFAFGPAWQGFTANLNVVDEAYDGTLKTYVDLNVQNLKKMIPGFRQIERVPFKTLSGL
                     SGERIRAEDTQSQRELRQSFYLIASGKGRMFVLTGTSLASDGPKFDSLFDDSADSFRL
                     GK"
     gene            1002..1074
                     /locus_tag="PK362_03975"
     tRNA            1002..1074
                     /locus_tag="PK362_03975"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1035..1037,aa:Ile,seq:cat)
     gene            1118..1189
                     /locus_tag="PK362_03980"
     tRNA            1118..1189
                     /locus_tag="PK362_03980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1150..1152,aa:Val,seq:cac)
     gene            1224..1949
                     /locus_tag="PK362_03985"
     CDS             1224..1949
                     /locus_tag="PK362_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNC74031.1"
                     /translation="MNEKIASLIRLQEMDKELDALRASVDRLSPERESLKAQIAASAA
                     RFDAGKKSLTDAQVQKKNLEIEIDTQDQQVRKHSGQLNSVKSNDAYKALLLEIEAAKK
                     QKSDLEDQVLVLMETIEKLQKQSKEDDVAAQRERADIEKKIAALDAEEAQGRAAVAAK
                     TAERDAFAAAAPADARSRYESVQRGRPGFVVVVPVNGMTCGGCRTLLTADTVNQAMKG
                     KEIVACDSCSRLLFIVPKPAVAS"
     gene            1946..2404
                     /locus_tag="PK362_03990"
     CDS             1946..2404
                     /locus_tag="PK362_03990"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNC74032.1"
                     /translation="MTQLPLFPSAADGVWTAYCDGASRGNPGPASFGLVVVDPADRTV
                     RELGVAIGINTNQVAEYEGLIRALEELSRAGARRARVVTDSQFVVKQFNGEYRAKDER
                     MKALLARAKAVAARFDALTVQHVMRSGHAHNTRADALANRALDEAKKKTH"
     gene            2407..2824
                     /gene="rnpB"
                     /locus_tag="PK362_03995"
     ncRNA           2407..2824
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK362_03995"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2891..>3408
                     /locus_tag="PK362_04000"
     CDS             2891..>3408
                     /locus_tag="PK362_04000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC74033.1"
                     /translation="MTKKIPAALWFGLLPVLLGLPFIGRAYFVDDHYHLLMARGLLEH
                     PTRPYDFLADDAGVDNVGWERGQPPRMVNPPLHHYLLALFWKITGGRLWAVRLLALFV
                     SGAAIVFLYLLAQRFAVPPGPAAALAALTPAFWLSSYALLIDSTMLVFFLGGLWGWFE
                     GLRRRSAGCLMFA"
BASE COUNT          612 a         1118 c         1090 g          588 t
ORIGIN      
        1 ccatgacgtg tgggaagcct tggtcgacgc ggggaagctc cggcgcttcg acggcggctt
       61 ctacgggcta cccgcgcacc ttggaggaac tgggccgcat cttcagcgtc acccgggagc
      121 gcgttcgcca gatcgaagcc aaggccattc gaaaacttcg gcacccgagc cgcagccgtt
      181 ttctccgcga atatgtggaa taaggccgcc gttctttcct tggccgtcct ggtcgccggg
      241 gcggcgcccg tccgggccga gctgggcgaa cgccacgtgg agcgcgcggg cggtttttcc
      301 tattgcccgc ccgagggttg gtccgtccgg gaattccccg ggttcaaata caaattcgcc
      361 ttcgggcccg cctggcaggg tttcaccgcc aacctcaacg tggtcgacga ggcctacgac
      421 ggaacgctca agacctacgt ggacttgaac gttcagaacc tcaagaagat gatccccgga
      481 ttccgccaaa tcgagcgcgt gccctttaaa acgctttcgg ggttgtccgg cgagcggatt
      541 cgggccgagg acacccagag ccaacgggag cttcgccagt ccttttactt gatcgccagc
      601 ggcaaaggcc ggatgttcgt tttgaccggg acctcgctgg ccagcgacgg gcccaagttc
      661 gactcgctct tcgacgacag cgccgacagc tttcgactgg ggaagtagcg gggtccgccc
      721 gtccggggcg gatggggtat aataaatcgg tggtgtttgg gatcgcgaat tctcagattc
      781 ggcggtgcga tgggtctggg ctcgatggaa gaattacagc tgatttgttg tggttgttgc
      841 ggcattgggc cgcgtttccg ataggcatcg tcccgattct ctttgcaggg tcccttttgt
      901 aagatggcgc ggtagtgtca gcggactgaa agcattaacc cgtcggcatt tgcgcggagc
      961 gcaaataccg gcggcgggtc cagaggaccc gaaaccccgc cgggccctta gctcaacggt
     1021 tagagcgtcc cgctcataac gggttggccg gtggttcaac tccaccaggg cccaaatttg
     1081 acaatctggt ataatcagcc ttcgtcgccg ctccggtggg tggttagctc agcggcagag
     1141 cgttcgcctc acacgcgaaa ggtcataggt tcaatcccta taccacccac cggagcgacg
     1201 gccgccggaa aacgaggaag accgtgaacg agaaaatcgc gtcgttgatc cgcctgcagg
     1261 aaatggacaa agagctcgac gcgctgcgcg cgtccgtgga ccgcctgtcg cccgaacgcg
     1321 aatccttgaa agcccagatc gccgcctccg ccgcccgctt cgacgccggc aagaaatccc
     1381 tcaccgacgc ccaggttcag aagaaaaacc tcgaaatcga aatcgacacc caggaccagc
     1441 aggttcggaa acattccggc caattgaaca gcgtcaaatc caacgacgcc tacaaggccc
     1501 tcctgctgga aatcgaagcg gccaaaaagc agaaatccga tttggaggac caggtcctgg
     1561 tcctgatgga aaccatcgag aaactccaga aacaatccaa ggaagacgac gtcgccgctc
     1621 agcgggagcg ggccgacatc gaaaagaaga tcgccgccct ggacgccgaa gaggcccagg
     1681 gccgcgccgc ggtcgccgcc aaaacggccg agcgggacgc cttcgccgcc gcggccccgg
     1741 ccgacgcccg gagccggtac gaatccgtcc agcgcggccg gcccggtttc gtggtggtgg
     1801 tccccgtcaa cggcatgacc tgcgggggat gccgcacgct gctcacggcc gacaccgtca
     1861 accaggccat gaagggcaag gaaatcgtgg cctgcgacag ctgttcgcgc cttctgttca
     1921 tcgttcccaa acccgccgtc gcctcctgac ccaactccct ctttttcctt cggccgccga
     1981 cggtgtttgg acggcctatt gcgacggcgc ctcccggggc aacccgggcc cggcctcctt
     2041 cggcctggtg gtcgtcgatc cggcggaccg gaccgtccgg gagctcgggg tcgccatcgg
     2101 gatcaacacg aaccaagtgg ccgagtacga ggggctgatc cgcgccctgg aggaattgtc
     2161 ccgggccggc gcccggcgcg cccgcgtcgt cacggattcc caatttgtcg tcaagcagtt
     2221 taacggcgag taccgcgcca aggacgaacg catgaaggcc ttgctggccc gggccaaggc
     2281 cgttgccgcc cgcttcgacg cgttgacggt gcagcacgtg atgcgctcgg ggcacgccca
     2341 caacacccgg gcggacgcgc tggcgaaccg ggccttggac gaagccaaga aaaagacgca
     2401 ctgatttacg gcggccgccg gatcgccgcg gcgaacacgg ccgcggagga aagtccgagc
     2461 tccgggggct tcggccccgc agggcgggac gcttcccaac gggaaggcgg ggggtggcgt
     2521 ttgcccccga cggaaagtgc cacagaaaac acaccgccca agacccccgc gaggggggac
     2581 ggcaagggtg aaatcgcgag gtaagagctc acggtccccc gtcgagagac ggggggcggc
     2641 aaaccccgtc cggagaaagg tcgtagggaa cgcgcgcggg ccggcccggc ccccggcccc
     2701 cgcaaggggg tcgacgttcc cggtggaccg cttgagcccg acggcgacgt cgggcccaga
     2761 gaaatgatcc ggccccgtcg gtcgccgacg gggacaggac tcggtgtaca ggccgccgca
     2821 aacgccaacg gggccatgga tcgccaacgc gttccatggc cccacttttt tgattcgcta
     2881 taatgccccc gtgaccaaaa agatccccgc cgcgctctgg ttcggccttc tcccggttct
     2941 gttgggcctc ccgttcatcg ggcgggccta ttttgtcgac gaccactacc acctgctcat
     3001 ggctcgcggc ctgctggagc accccacgcg gccctacgac tttctcgccg acgacgccgg
     3061 cgtggacaac gtcggttggg agcggggcca gccgccccgc atggtcaacc cgcccctgca
     3121 ccattatctt ttggccctct tctggaaaat cacggggggc cgtttgtggg ccgtccgttt
     3181 gctggccctt ttcgtctccg gggcggcgat cgtttttctg tatttgctgg cccagcggtt
     3241 cgcggtgccg ccgggccccg ccgccgccct ggcggccctg acgccggcct tttggctgtc
     3301 ttcctacgcc ctgttgatcg actccacgat gctggtcttt tttctgggcg gtctctgggg
     3361 ctggtttgaa ggcctgcgac gccggtcggc cggatgcctg atgttcgc
//
LOCUS       DAOQLS010000157         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15157_length_3364_cov_4.438501, whole genome shotgun sequence.
ACCESSION   DAOQLS010000157 DAOQLS010000000
VERSION     DAOQLS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15157_length_3364_cov_4.438501"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..309)
                     /gene="rpmE"
                     /locus_tag="PK362_04005"
                     /pseudo
     CDS             complement(121..309)
                     /gene="rpmE"
                     /locus_tag="PK362_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512349.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            complement(397..1341)
                     /locus_tag="PK362_04010"
     CDS             complement(397..1341)
                     /locus_tag="PK362_04010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC74034.1"
                     /translation="MRRQTDVLVGGIFAAAALVVTLAPVGGASFQFSAGEAPAFPESY
                     LLTLAPASAGPSPATFQALKDMGRVVVGQYEVRPQDTLASIAATYGSTADYLRSTNHL
                     ESTKLPPGKTLLVHSGKGMLYQVREKKGRSENLRQIAQRYNRSAVEIARANRLPGVAL
                     LGDFELADGSVLFLPGVRLRFTDYMVPVAWVPGKRMISSGFGVRRHPLLRTRRFHTGL
                     DMPRPYGFPVKASREGTVIFCGWRGGYGKLVIIKHQGGLRTWYGHLSQIDVTEGQRVA
                     KGHLVGRVGATGLATGPHLHFEVRDRFGNSLNPKKFIF"
     gene            complement(1428..2759)
                     /gene="rho"
                     /locus_tag="PK362_04015"
     CDS             complement(1428..2759)
                     /gene="rho"
                     /locus_tag="PK362_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNC74035.1"
                     /translation="MESHTHTPHSHAASAGGNHKPLNAEALTKASMSEIIKIGEDLKI
                     PAIAGMRKQEAIARILEAQARDSNNGAIVKGGVLEVLQDGFGFLRSPDYNYLPGPDDI
                     YISPSQIKKFGLRKGDTVTGQVRPPKDQERFFALLQVQTINGEPVEKAIHRPLFDNLT
                     PLYPQNRINLETKKDEINTRILNLVTPIGKGQRALIVAPPRTGKTIFLQKIANSITEN
                     HPEIDLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPQDRHVQVAEMVLEKARRQVEC
                     GKDVLILLDSITRLARAYNSVTPSSGRVLSGGIDANTMERPKRFFGAARNIEEGGSLT
                     IIATALVETNSRMDDVIFEEFKGRGNMEVYLDRKLADRRVFPAIDIHRSGTRKEELLL
                     TSDELNKVWILRKVLSSLSPVDAMELLIDKVGATKSNKDFLRSMELSSMEK"
     gene            3010..>3364
                     /locus_tag="PK362_04020"
     CDS             3010..>3364
                     /locus_tag="PK362_04020"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HNC74036.1"
                     /translation="MVHVIETQATPNPDSLKFITDAALVAQGAQSFESPEAAKGHALA
                     EAIFSSGPVASVFVMDRFVTITKFPDADWPLIKARLVAAIETTAAPAAAAGAAAAPGS
                     TDEKLALINAVIDENV"
BASE COUNT          608 a         1005 c         1082 g          669 t
ORIGIN      
        1 ggcgcccgct ttgggcgtgg tgcggagaac tttgtcggct tttttggccg cggggcgcgc
       61 cggagcggcc gcctttttgg ccgcgggctt gcggaccacg gttttgccgc cggtggcggc
      121 gtagcgcttc tcaaaacgct ccacgcgacc cgcggtgtcg acgagcttct gacgacccgt
      181 gtagaacggg tggcaggcgg agcaaatttc aaggcggatc tgggacgcgg tggaacgggt
      241 gacaaagctg ttcccgcagg cgcaggccac ggtgacgaca ttgtaattgg ggtgaatgcc
      301 ttctttcatt tcaacctctc tatggggaga cgcgtttcca acaaaaagcc ccggcgggca
      361 aagccccgcg gggcgtccag gaatggctta ttatacctaa aaaatgaact ttttggggtt
      421 taaggaattg ccgaaacggt cgcgcacttc gaagtgaagg tgggggcccg tggccagccc
      481 cgtggcgccg acccggccga ccaggtggcc tttggcgacc cgctggcctt cggtcacgtc
      541 gatttgggaa agatgcccgt accaagtgcg gaggccgccc tggtgcttaa tgatcaccag
      601 tttgccgtat cccccgcgcc agccgcagaa aatcaccgtg ccctcccggg acgcctttac
      661 ggggaaaccg taggggcggg gcatgtccaa ccccgtgtgg aatcgccggg tgcggagcaa
      721 cggatgtcga cgaacgccga accccgaaga aatcatgcgc ttgcccggaa cccaggcgac
      781 ggggaccatg tagtccgtga atcgaaggcg cacccccggc aaaaacagca cgctgccgtc
      841 cgccagctcg aaatcgccca gaagggcgac ccccgggagt cggttggccc gggcgatttc
      901 caccgccgac cggttgtacc gttgggcgat ttgccgaaga ttttcggacc ggcctttttt
      961 ctcccgaact tggtagagca tgcctttgcc gctgtggacc aggagggttt ttccgggcgg
     1021 aagtttggtg gattccaaat ggttggtgct gcgaaggtaa tccgcggtgg acccgtaggt
     1081 ggcggcgatg ctcgccaggg tgtcctgggg acggacctcg tactggccga cgaccacgcg
     1141 acccatgtct ttcaacgctt ggaaggtggc cggggagggc ccggcggaag cgggcgcgag
     1201 ggtcaggagg tacgactccg gaaaagcggg cgcttcgccg gcgctgaatt ggaacgacgc
     1261 cccgcccacg ggcgccaggg tgaccaccag cgccgcggcc gcgaaaatac cgcccaccaa
     1321 gacatcggtt tgtcgacgca taagctctag ttaaggatac cccgatttcc gattattttc
     1381 aataaaaatc cgggggccga aatcgccaaa agggggcgat ttatcgccta cttctccatg
     1441 ctggacaatt ccatggagcg caggaagtcc ttgttggatt tcgtggcgcc caccttgtcg
     1501 atcaggagtt ccatggcgtc caccggggag aggctcgaga gcactttgcg gagaatccac
     1561 actttgttga gctcgtcgga ggtcaggagc aactcttcct tccgggtgcc cgatcggtgg
     1621 atgtcgatgg ccgggaacac gcggcggtcg gccagcttgc ggtccaaata gacctccatg
     1681 ttgccgcggc ctttgaattc ctcgaagatc acgtcgtcca tgcggctgtt ggtttccacc
     1741 agcgccgtgg cgatgatcgt gagggacccg ccctcctcga tgttccgggc ggcgccgaag
     1801 aaccgcttcg gccgctccat ggtgttggcg tcgataccgc cggaaagcac gcggcccgag
     1861 ctcggggtca ccgagttgta ggcccgggcc agccgcgtga tcgagtccaa caggatcaaa
     1921 acgtccttgc cgcactccac ctgccggcgg gctttttcca acaccatttc ggccacctgc
     1981 acgtggcggt cctggggttc gtcgaaagtc gagctgatga cttccccctt cacgctccgc
     2041 tgcatgtccg tgacttcctc ggggcgctcg tcgatcaaca gaacgatcag gtcgatttcg
     2101 ggatggtttt ccgtgatgga gttggcgatc ttctggagga agatcgtttt gccggtccgg
     2161 ggcggggcga cgatcagcgc gcgctggcct ttgccgatgg gcgtcaccag gttcaggatt
     2221 cgggtgttga tttcgtcttt tttggtttcc aggttgatgc ggttttgggg gtagaggggc
     2281 gtcaggttgt cgaagagggg ccggtggatg gctttctcga cgggctcccc gttgatcgtc
     2341 tggacctgga ggagggcgaa gaaccgttcc tggtccttgg gcggccgcac ctggccggtc
     2401 accgtgtcgc ctttccgcag gccgaacttt ttaatctggc tcggcgaaat gtagatatcg
     2461 tccggacccg gcaaataatt gtagtccggg ctccgcaaaa aaccgaaccc gtcctgaagc
     2521 acttccaaca cgccgccctt cacgatggcg ccgttgttgg aatcccgggc ctgggcttcc
     2581 aaaatgcgcg caatggcctc ctgtttccgc atgccggcga tggcgggaat tttcaaatcc
     2641 tcgccgatct tgatgatttc ggacatggac gccttggtca gggcctcggc gttcaggggc
     2701 ttgtggttcc cgccggcgga cgccgcgtgg gaatgggggg tgtgggtgtg tgactccatg
     2761 aagcactcct tagagttggg ggatgggtgt ctgcgtgttt cgatcaaacg atggaaaaac
     2821 ccgcggccgc gccgtgcgcg ccgttgggga tgttcacctc ggaatggaca acggaattgg
     2881 gaagagcatc tcggtgggtt tttccgcact cattatacac gaccgacgat cgctgtcaag
     2941 ggttttcccg gacctggacc ggggccgtcg aaaaagatac aatacatcga cggtaaaaag
     3001 gagaacccca tggtccacgt gatcgaaacc caagccaccc ccaaccccga ttcgctcaaa
     3061 ttcatcaccg acgcggccct ggtcgcccag ggcgcccagt ccttcgaatc gcccgaagcc
     3121 gccaagggcc acgccctggc cgaggcgatt ttttcctccg ggcccgtggc ctcggtgttc
     3181 gtcatggacc ggttcgtcac catcaccaaa tttccggacg ccgattggcc cctgatcaaa
     3241 gcgcgcctcg tggccgccat cgaaaccacc gcggcgccgg ccgcggccgc gggagccgcc
     3301 gcggccccgg gctccacgga cgaaaaactg gccctcatca acgcggtcat cgacgagaac
     3361 gtcc
//
LOCUS       DAOQLS010000158         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15211_length_3355_cov_5.294545, whole genome shotgun sequence.
ACCESSION   DAOQLS010000158 DAOQLS010000000
VERSION     DAOQLS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15211_length_3355_cov_5.294545"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PK362_04025"
     CDS             <1..791
                     /locus_tag="PK362_04025"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNC74037.1"
                     /translation="YFERLFTGLIELHGDRHYADDKAIVGGLAYLDGQPVVVIGHQKG
                     RTLQESMARNFGMPHPEGYRKALRLMRLAAKFGAPVVCLIDTAGAYPGIGAEERGQAT
                     AIAENLREMSQLPVPILCAVIGEGGSGGALAIGVGDRLLMLENAWYSVISPEGCASIL
                     YRDAAKAPDAARALKITAQDLKGIGIVDEIVPEPVGGAHRDPDAAAAALKAALVRHLK
                     ELRALPLDKLLDGRYDKYRAVGHFEETEKKKAKPAARRRSKTKA"
     gene            822..1901
                     /locus_tag="PK362_04030"
     CDS             822..1901
                     /locus_tag="PK362_04030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNC74038.1"
                     /translation="MSQVVEAAKKSGTTAEMLGKDAETLLTYKAKVPASRLHLPGPDF
                     VDRVFAQSDRGPAVLRNYQHTMNTGRLAGTGYVSILPVDQGIEHSGGASFAPNPDYFD
                     PENIIKLAIEGGCNAVASTYGVLGAVARKYAHKIPFMLKINHNELMSLPQVPDQTLFT
                     KVKRAFDMGCTSIGATIYWGSPESRRQLQEISEAFAQAHELGLFTVLWCYVRNNAFKT
                     PEKDYHVSADLTGQGNHLGVTIEADIIKQKLPENNGGYNAVKFGKTSPKVYSDLTTDH
                     PIDLTRYQVMNCYMGRAGLINSGGESKGASDLAEAVRTAVINKRAGGMGLISGRKAFQ
                     KPLREGVQLLNAVQDVYLDKSVTIA"
     gene            2014..>3355
                     /locus_tag="PK362_04035"
     CDS             2014..>3355
                     /locus_tag="PK362_04035"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNC74039.1"
                     /translation="MSTTTSALQIERLKFKPALPKVLRAGPSGVTAKAGRPTQSLSDQ
                     ASVQGLFPRTYGLPLVSFVPGKNPAAAKKAVRVGVVLSGGQAPGGHNVLAGLLDGLKK
                     ANPKNKLFGFLGGPSGILENKFKELTPALVAGYRNTGGFDLIQSGRTKIETPEQFAAA
                     KKNIADNQFDALVVVGGDDSNTNAALLAEYFKAEGLAAAVLGVPKTIDGDLKNDQIEA
                     SFGFDTATKIYAEVVGNIGRDILSARKYWHFVRLMGRSASHITLEVALQTHPNLALIG
                     EEVLEKKQTLADVVDGIAQTVARRAADKKNYGFVLIPEGLIEFIPEMRSLISGLNDAL
                     AQNEGALAGLSAFEEKRDVVAKSLPAPLAGLLRSLPDGFQAQLMLDRDPHGNVTVSQI
                     ETEKLLVEMVKKRLAEMKKAGSYDGKFAAITHFFGYEGRCGAPSNFDATYCYALG"
BASE COUNT          693 a         1112 c          978 g          572 t
ORIGIN      
        1 actattttga acgcctcttc accggcctca tcgaattgca cggcgaccgc cattacgccg
       61 acgacaaagc catcgtcggg ggcctggctt atttggacgg ccagccggtg gtggtgatcg
      121 gccaccaaaa gggacggacc cttcaggaat ccatggcccg caatttcggc atgccccacc
      181 cggaaggcta ccgcaaagcc ctccgcctta tgcgtctggc cgcgaaattc ggcgccccgg
      241 tcgtctgtct gatcgacacc gccggggcct accccggcat cggggccgag gaacggggcc
      301 aggcgacggc catcgcggaa aatttacggg aaatgtccca gctccccgtg cccatcctgt
      361 gcgccgtcat cggcgaaggc ggttcggggg gggctctggc catcggcgtg ggggaccggc
      421 tgttgatgct cgaaaacgcc tggtactccg tcatttcccc ggaaggctgc gcctccatcc
      481 tttaccggga cgccgccaag gccccggacg cggcccgggc gctcaaaatc acggcccagg
      541 atttgaaagg catcggcatc gtcgacgaga tcgtccccga acccgtgggc ggcgcccacc
      601 gggaccccga cgcggcggcc gcggcgctca aggccgccct ggtccgccac ttgaaggaat
      661 tgcgcgccct gcccctggac aagcttttgg acggtcggta cgacaaatac cgcgcggtgg
      721 gtcatttcga ggaaaccgag aagaagaaag ccaaacccgc cgcccggcgg cgatccaaaa
      781 ccaaagcctg attttggtcc attgaaaaag gagaagtcat catgtcccag gtagtcgaag
      841 cggcaaagaa gtccggaacc acagcggaaa tgttgggcaa agacgcggaa acccttttga
      901 cgtacaaggc gaaggtcccg gcctcccgcc tgcacctccc cggccccgat ttcgtcgacc
      961 gcgtgttcgc tcagtcggac cgcggccccg ccgttctccg gaactatcag cacaccatga
     1021 acaccggtcg tctcgcgggc accggatacg tgtccattct gcccgtcgat cagggcatcg
     1081 aacactccgg cggcgcgtcc ttcgccccga acccggatta ttttgatccc gaaaacatca
     1141 tcaagctcgc catcgagggc ggttgcaacg cggtcgcctc gacctacggc gtcctgggcg
     1201 ccgtggcgcg caaatacgcc cacaagattc ccttcatgct caagatcaac cacaacgagc
     1261 tcatgagcct gccccaggtg ccggatcaga cgctcttcac gaaggtgaaa cgcgccttcg
     1321 acatgggctg cacctccatc ggcgcgacca tctactgggg ctcgcccgaa agccgccgcc
     1381 agcttcagga aatttccgag gctttcgccc aggcccacga actcggcctc ttcaccgtgc
     1441 tgtggtgcta cgtgcggaac aacgccttca agaccccgga aaaggactac cacgtctccg
     1501 ccgatctcac gggccagggc aaccacctgg gcgtgaccat tgaagccgac atcatcaagc
     1561 agaaactgcc cgagaacaac ggcggttaca acgccgtcaa attcggcaaa acgagcccga
     1621 aggtttactc cgacctcacc acggaccacc ccatcgacct cacccgctac caggtgatga
     1681 actgctacat gggccgcgcc gggttgatca actcgggggg cgaatccaag ggcgcctcgg
     1741 atctggcgga agcggtccgc acggccgtca tcaacaagcg cgccggcggc atgggcttga
     1801 tttcgggccg caaggccttc caaaagcccc tccgcgaggg cgtccagctc ctgaacgccg
     1861 ttcaggacgt ctacctggac aagtcggtca ccatcgccta aggcgatttt caagccccca
     1921 tgcccgaacg tcgttcgggc atgggggtct ttgttttcaa gcgacgcata ttcccgtcgg
     1981 ccgcccccgg cggcccattg tcgagaggat cgcatgtcca cgaccacatc cgcgcttcaa
     2041 atcgaacgac tcaaattcaa acccgccctg cccaaagtgc tccgggcggg accgtccggc
     2101 gtcacggcga aggccggccg gccgacgcaa agtttgtcgg accaagcctc ggtccagggc
     2161 ctctttcccc ggacctacgg gcttcccctc gtttcctttg tccccggaaa aaacccggcc
     2221 gcggccaaaa aggccgtgcg ggtcggcgtg gttctttcgg gcgggcaggc cccgggcggg
     2281 cacaacgtgt tggcgggact tctggacggt ctcaaaaaag cgaaccccaa aaacaaattg
     2341 ttcggtttcc tgggcgggcc ttcggggatc ctggaaaaca agtttaagga actgacgccg
     2401 gcgctcgtgg ccgggtatcg gaacaccggc ggtttcgatt tgattcaatc gggccggacc
     2461 aaaatcgaga caccggaaca gttcgccgcg gccaagaaaa acatcgcgga caaccaattc
     2521 gacgccctgg tcgtggtggg gggggacgat tccaacacca acgccgcctt gctcgcggaa
     2581 tatttcaagg ccgagggcct cgcggccgcc gttctgggag ttcccaaaac catcgacggg
     2641 gatttgaaga acgatcaaat cgaagcgtct ttcggtttcg acacggccac caagatctac
     2701 gccgaggtcg tcggcaacat cggccgcgat attttgtcgg cccgaaaata ctggcacttc
     2761 gtccgcctca tggggcggag cgcctcccac atcaccctgg aagtggccct ccaaacccat
     2821 ccgaacctgg ccctgatcgg cgaggaagtg ttggaaaaaa agcagaccct ggccgacgtc
     2881 gtggacggca tcgcgcaaac cgtcgcgcgc cgggccgcgg acaagaagaa ttacgggttc
     2941 gtcctcattc ccgaggggct catcgagttc attcccgaaa tgcgctctct catatcggga
     3001 ttgaacgacg ccctggccca aaacgagggc gctctggccg gtctctcggc ctttgaggaa
     3061 aagcgggacg tggtggcgaa atctctcccg gcgcccctgg ccgggctgtt gcgatctttg
     3121 cccgacgggt ttcaggctca attgatgctc gaccgggacc cccacgggaa cgtgaccgtt
     3181 tcccaaatcg aaaccgaaaa gctcctggtg gaaatggtca aaaagcgcct ggcggaaatg
     3241 aaaaaagccg ggtcttacga cgggaaattc gccgccatca cccatttctt cggctacgag
     3301 ggccgttgcg gcgcgccgtc caacttcgac gcgacctatt gctacgccct gggat
//
LOCUS       DAOQLS010000159         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15238_length_3352_cov_3.441614, whole genome shotgun sequence.
ACCESSION   DAOQLS010000159 DAOQLS010000000
VERSION     DAOQLS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15238_length_3352_cov_3.441614"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PK362_04040"
     CDS             <1..280
                     /locus_tag="PK362_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC74040.1"
                     /translation="YLPSKSDNISVKGVGLVLAEKLTKGTILALSIHMTEEKQKISAI
                     ARVVWSQPTDISHHFLTGLEFIVVYKKTRSATEYVDSSVMQKLLHPPR"
     gene            369..1439
                     /gene="bioB"
                     /locus_tag="PK362_04045"
     CDS             369..1439
                     /gene="bioB"
                     /locus_tag="PK362_04045"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNC74041.1"
                     /translation="MNPPVLDRLDPRALADKSLSGAPLTRDEARAVLAWPDEDVLSLL
                     NAAHRVRVAHFGRRVKLNYLVNLQSGLCAEDCSYCTQSKISKAPVEKYRMMSAQEVMD
                     LADRAVASGAARLCLVASMRGPSERDIAGLEKTVRAVKEKHPGLEICASLGLLKDGQA
                     ERLADSGVDAYNHNLNTSERHYGEICHTHTHQDRVDTVGRAKSGGLSPCSGALFGMGE
                     TPEDILDVAYRLREMKVDSIPVNFLIPVAGTPMANRNDVTPTAALKILCLFRFLCPDS
                     ELRVAGGRELHLRSLQPLGLFVANSIFVGDYLTTKGQAAQADRDMIRDLGFEIQGDTA
                     SAEASGASPADRVEMVTRATRQ"
     gene            1436..2155
                     /locus_tag="PK362_04050"
     CDS             1436..2155
                     /locus_tag="PK362_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC74042.1"
                     /translation="MNVLVTGSSRGIGRRLALAFGRAGHSVVVHYRARRGEAEAVLGE
                     LQAAGVRSDAAAADLADPLQCRELIDGVIARWGRLDVLVNNAGATKDRALLNMTPEEW
                     RAVVDVNLSGAFYCLQAAAKHMVREKSGAVINVGSIVGVRGGVGCANYAAAKAGLLGL
                     TKAAAKELGRFNVRVNAVLPGFHPTEMSDSLPAAVKDKVVAAHALGRPTDLADLEKLV
                     VLLAELPSVSGQIFNVDSRLL"
     gene            2152..3276
                     /locus_tag="PK362_04055"
     CDS             2152..3276
                     /locus_tag="PK362_04055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNC74043.1"
                     /translation="MTTRPDRERVAVTGLGLVTPLGDNRESSWARLTGGDSAVGPAGA
                     RVPLPDVRHRALAWAERAAAEALAEVGDAWTGGERCGCSVSGSKPLLSEFSRNALDWR
                     PPEDVCSTVARLGRVGGPVINLSSACATGAQSVLVAAGWIREGRVDRALAGAAESSFH
                     PLYVAGFDQMGVLSPGRRVRPFDRARDGFLLGEGAGVFVLERWDLARARGAAIYAEVS
                     GGDFSCDAHHATRFNSQGRRMADCLRRALTRAGASPSDLDYVNAHGTATLLNDALETR
                     ALESLFQEGGAPPVSSTKGATGHLLGATGAVELGFTLLALRDGRLPPTLNLENPETES
                     IDFVPGRSRPAVLRRAASLSFGFGGALASVVLEKPECAPR"
BASE COUNT          515 a         1161 c         1096 g          580 t
ORIGIN      
        1 ctaccttccc tccaaatccg acaacatcag cgtcaaaggg gtcgggttgg tcctggccga
       61 gaaactcacc aagggaacga ttctcgcctt gtccattcat atgacggagg agaaacagaa
      121 gatctcggcc atcgcccgcg tggtgtggtc ccaacccacg gacatttccc atcattttct
      181 caccggcttg gaattcatcg tggtttacaa gaagacccgt tccgccacgg agtatgtgga
      241 ttcctcggtg atgcaaaaac tcctccaccc gccccgctga tccattgtca acctaccggc
      301 gaatcgccgg ttgacaatcc ccgctccgtt tggtttcatg gactcctatt tccgtctaag
      361 gagaccccat gaatcccccc gttctcgacc gtttggaccc ccgcgccctg gccgataaaa
      421 gcctgtccgg cgcccccctg acccgcgacg aagcccgggc cgtgctggcc tggcccgacg
      481 aggacgtcct gtccctcttg aacgccgccc accgggtgcg cgttgcccac ttcggccgcc
      541 gggtgaaact caactacctg gtcaacctcc agagcggcct gtgcgccgag gactgttcct
      601 attgcaccca atccaaaata tcgaaggccc cggtggagaa ataccggatg atgagcgccc
      661 aggaagtcat ggacctggcc gaccgggccg tggcctcggg cgccgcgcgc ctctgcctgg
      721 tggcgtccat gcgcggccct tcggagcggg acatcgcggg gctggaaaag acggtccggg
      781 ccgtgaagga aaaacatccg ggcctggaaa tctgcgcctc cctgggcctc ttgaaagacg
      841 gtcaggcgga gcggttggcg gactcgggcg tcgacgccta caaccacaac ctcaacacca
      901 gcgaacgcca ttacggcgaa atttgccaca cccacaccca tcaggaccgc gtggacaccg
      961 tcggccgggc caaaagcggc ggcctctcgc cctgcagcgg cgccctcttc ggcatgggcg
     1021 agactcccga ggacattctg gacgtggcct accggttgcg ggagatgaag gtggattcca
     1081 tccccgtcaa tttcctgatt cccgtggcgg gaacgccgat ggcgaaccgc aacgacgtga
     1141 cgcccacggc cgccctgaag attctctgcc tcttccgatt cctctgcccc gactcggagc
     1201 ttcgcgtcgc gggcgggcgg gaactgcacc tacgctccct gcaacctttg gggctcttcg
     1261 tcgccaactc catcttcgtg ggggattacc tgaccaccaa gggccaggcc gcccaggcgg
     1321 accgggacat gatccgggac ctgggcttcg agatccaggg ggacaccgct tccgccgagg
     1381 cctccggcgc ttcgccggcc gatcgggtgg agatggtcac ccgcgccacc cgccagtgaa
     1441 cgtgttggtc acggggtcca gccggggcat cggccgccgg ttggccctcg ccttcggccg
     1501 ggccggccat tcggtggtcg tccattaccg cgcccgccgg ggggaagccg aagccgttct
     1561 gggcgagctg caggccgccg gcgtccgttc ggacgcggcc gccgcggatc tcgccgatcc
     1621 cctccagtgc cgggaattga tcgacggcgt gatcgcccgc tggggccggc tcgacgtttt
     1681 ggtcaacaac gccggggcga ccaaagaccg ggcccttttg aacatgaccc ccgaggagtg
     1741 gcgagcggtc gtggacgtca atttgtccgg ggcgttctat tgccttcaag ccgcggccaa
     1801 gcacatggtc cgggaaaaat ccggcgccgt gatcaacgtg ggatccatcg tcggggtccg
     1861 gggcggcgtg ggttgcgcca actacgcggc cgccaaggcg gggctcctgg gtttgaccaa
     1921 ggccgccgcc aaggaactgg gccgattcaa cgttcgcgtc aacgccgttc ttccgggttt
     1981 ccaccccacc gaaatgtccg attcccttcc cgccgccgtc aaagacaagg tcgtggccgc
     2041 ccacgcgttg ggacgtccca cggacctggc cgacctggag aaattggtgg ttttgttggc
     2101 cgagttgccc tcggtgtcgg ggcaaatctt caacgtggat tcccgactgt tgtgacgacg
     2161 cgaccggacc gggaacgggt cgccgtcacg ggcctggggc tcgtgacgcc cctgggcgac
     2221 aaccgggaaa gctcctgggc ccgcctgacg ggcggcgaca gcgccgtggg ccccgcgggc
     2281 gcccgggtgc ccttgccgga cgtccgccat cgggccctgg cctgggcgga acgggccgcg
     2341 gccgaggccc tggccgaggt gggcgacgcc tggacgggcg gcgaacgatg cggctgttcc
     2401 gtgagcggca gcaaaccgct cctctccgaa ttttcccgga acgccctgga ctggcggccg
     2461 cccgaggacg tgtgctccac cgtggcgcgc ctggggcggg tcgggggccc ggtgatcaat
     2521 ttgtcgtcgg cctgcgccac cggggcccag tcggtgttgg tggccgccgg gtggatccgc
     2581 gaggggcgcg tggaccgggc cctggccggg gccgccgaat cgtcttttca tccgctctac
     2641 gtggcgggct tcgaccaaat gggcgtgctt tcgccgggcc gccgggtgcg ccccttcgac
     2701 cgggcccggg acggttttct cctgggcgaa ggggccgggg ttttcgtttt ggagaggtgg
     2761 gacctggccc gggcccgggg cgcggccatc tacgccgaag tgtcgggggg ggatttttcc
     2821 tgcgacgccc accacgccac gcggttcaac tcccagggcc gccgcatggc cgattgccta
     2881 aggcgcgcct tgacccgggc cggggcgtcc ccgtcggatt tggactacgt gaacgcccac
     2941 ggcaccgcga cccttttgaa cgacgccctg gaaacccggg ccctggagtc cctctttcaa
     3001 gaaggcggcg cgccgccggt gtcctccacc aagggcgcca cgggacattt gttgggcgcc
     3061 accggcgcgg tggaattggg atttaccctc ctggccctgc gcgacgggcg actgccgccg
     3121 accctcaact tggaaaaccc cgaaaccgaa tccatcgact ttgtccccgg ccgttcgcgc
     3181 ccggcggtcc tgcgccgggc cgcctccctg tccttcggtt tcggcggggc cctggcctcc
     3241 gtggttctgg aaaaacccga atgcgctccg cgctgaccga ccggttgtcc cgggcgctgg
     3301 accttcttcg gaaggaaaac cgctttcgcg atccccgccg cctttcggcg gc
//
LOCUS       DAOQLS010000160         3351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15244_length_3351_cov_4.527306, whole genome shotgun sequence.
ACCESSION   DAOQLS010000160 DAOQLS010000000
VERSION     DAOQLS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15244_length_3351_cov_4.527306"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK362_04060"
     CDS             <1..817
                     /locus_tag="PK362_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNC74044.1"
                     /translation="IPFEAVRVERDPLFGGVSPEPIERHLAALSGAVRAQKAVLGVAV
                     DGDGDRLGAVDEKGVYLPPHAVFPLILRHLVENRKLKGAVVQAVSLGYLSERLAKEYK
                     LPFVEVPVGFKYVADQMAKIRVLWGGEESGGYGVGLWGPERDGVLTGLLLVEAVLAAG
                     KPLSVLRKELTDKLGASEFSRVDHPLKAPVVDKAAWVATVTKRIPAKVGGVAVKETRN
                     TDGLKVVLTDGSWVLLRPSGTEPLLRAYAESPTAALTQQLLTKAQEWAGAKNI"
     gene            1182..2591
                     /locus_tag="PK362_04065"
     CDS             1182..2591
                     /locus_tag="PK362_04065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC74045.1"
                     /translation="MTPASLPESQPLMLASLMGRGRWAPTVVLFLGPVGLPLFGGLIV
                     WAMVRGPWPAADVFPLFVGLELLLYLGAGLGWSFGAALAATGLGLLGFFFSTAPQARL
                     LFAAHIALLWTVFAVLNLFDRRRQTAWNRFHEEWDGLEMDLAHTQTDVDSTRALIEDS
                     RARIVAFDRLQLFTDDLIGPYHREELLGKAQTGLSAMFPRARVSLHMFPKPDAPSPED
                     ELGMKMLQWGQPRLLASRAGTVPTWDQGLVIVLPVKGRENFLGWILLESKNPDAPFEI
                     HDLRLASIASDLIALALGNTERFSQTEALAISDELTGVYTRGYFNERLQEEFAKARHK
                     GRPFTLVLLDIDHFKKVNDLHGHHIGDEVLRWLARLVTGQARETDFVARYGGEEFVVL
                     MPNTRGADAFRFTQRLCKTIATTPFRWGAKKIKITLSAGVACLKDEVTAEEDLIRRAD
                     EALYAAKNGGRNRVCNYDA"
     gene            2581..>3351
                     /locus_tag="PK362_04070"
     CDS             2581..>3351
                     /locus_tag="PK362_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74046.1"
                     /translation="MTPELAHALLFWGAVPVLWVSIHWRDARGFLIAAVFGVFAVFHA
                     WGDAGALWDLAFHGGTLGLAALVPFYFRRYQSGLEREFARRKSHLSEQRSKSAHRHDA
                     IVREKNELQDNLERLQNRFALVQVMATKLEAGEILQTLGHMWKKRTGVKGCLILRRQL
                     NGNWSTAFTDGIFNAQDWIRFMTAHPSIARSRRIRRYAASARQLSFPGQPFGSTCLLI
                     PFTWDKDILAMGVVEVEPQRMEDSLEGFNIERKLVSIGL"
BASE COUNT          574 a         1068 c         1066 g          643 t
ORIGIN      
        1 gatcccgttc gaggcggtgc gcgtcgaacg ggatcccctg ttcggcggcg tgtccccgga
       61 acccattgag cggcatttgg cggcgttgtc cggagcggtc cgggcgcaga aggccgtgtt
      121 gggcgtcgcg gtggacgggg acggtgaccg tctgggcgcc gttgacgaaa agggcgtcta
      181 tctcccgccc cacgccgtgt tcccgctcat tttgcggcac ttggtcgaga accgcaaatt
      241 gaagggggcg gtggtgcagg ccgtttccct ggggtatctt tccgagcgtt tggccaaaga
      301 gtacaagctg ccgttcgtcg aagtcccggt cgggttcaaa tacgtcgccg atcaaatggc
      361 gaagatccgc gtcctgtggg gcggggagga atccggcggg tacggcgtgg gcctttgggg
      421 tcccgagcgg gacggcgtgc tgacgggtct cctgctggtg gaggcggtgc tggccgcggg
      481 caagccgctg tccgttcttc gcaaagagtt gacggacaag ttgggcgcct ccgaattctc
      541 ccgggtggat caccccctga aggcgccggt ggtggacaaa gccgcctggg tggcgaccgt
      601 gaccaaacgt attccggcca aagtcggggg tgtggcggtc aaggaaaccc ggaacaccga
      661 cggtcttaaa gtcgttttga cggacggctc gtgggtgttg ctgcggccca gcggcaccga
      721 accgcttctc cgcgcctacg ccgaatcgcc gaccgcggcc ctcacccagc aattgctgac
      781 gaaggctcag gaatgggccg gcgctaaaaa catttagccg cgtccgggcg tcgccccttc
      841 cgcggcgtct ccccgccgcc gtcggcgcgc tccacagaaa aaaccgccgc gcacgctaaa
      901 acgactctat ttgtagattc gttttgctat tgaccaattc ggtattcggg gtatacttaa
      961 gtgaagttta gccgagacct cgacacccat cgacatcacg attgacagcg acgacgcgga
     1021 tctccggccg aaaacgcggc gcgtttccgg gcggggtcgc ggaggggccg cgcgggggcg
     1081 ggatattttt ttggcggccg ggaatgggag gccctccgcc ggaggagctc cgatgagcgt
     1141 ccacgtccgg gaaggaacaa catgagccga cggtgccacc catgaccccg gcctctcttc
     1201 cggaatcgca gcctctgatg ttggcctcgt tgatggggcg gggccgttgg gccccgaccg
     1261 tcgtgctttt tttgggcccg gtgggtttgc ccctcttcgg cggactcatc gtctgggcca
     1321 tggtgcgcgg tccctggccc gccgcggacg tctttcccct gtttgtcggg ctcgaacttc
     1381 tcctctatct cggcgccggg ttggggtgga gtttcggcgc cgcgctcgcg gccaccggtc
     1441 tcggcctcct cggctttttc ttttccacgg ccccccaagc ccgcctcctc ttcgccgccc
     1501 acatcgccct tctttggacc gttttcgccg tcctcaattt gttcgaccgt cgacgacaaa
     1561 ccgcctggaa tcgatttcac gaagaatggg acgggctgga aatggatttg gcccacacgc
     1621 aaaccgacgt ggacagcacc cgggccttga tcgaggacag ccgcgcccgg atcgtggcct
     1681 tcgatcggct tcagctgttc accgacgatc tcatcggtcc ctatcaccgc gaggaacttc
     1741 tcggcaaagc ccaaacgggc ttgagcgcca tgttcccccg ggcgcgggtc tccctccaca
     1801 tgtttcccaa acccgacgcc ccgagccccg aggacgaatt gggcatgaaa atgctccagt
     1861 ggggtcagcc gcggcttctc gcctcccgcg ccggaaccgt gccgacctgg gaccagggac
     1921 tggtcatcgt cctgccggtc aaaggccggg aaaacttctt gggctggatc ctgctggaat
     1981 ccaagaatcc ggacgcgccc tttgaaatcc acgatttgcg cctggcctcc atcgcctcgg
     2041 atttgatcgc cctggccctg ggcaacaccg agcgcttctc ccagacggaa gccctcgcga
     2101 tttcggacga actgaccggc gtgtacaccc ggggctattt caacgaacga cttcaggaag
     2161 agtttgccaa ggcccggcac aagggccggc ccttcacgct cgtgttgctc gacatcgacc
     2221 atttcaaaaa ggtgaacgac ctgcacggcc accacatcgg cgacgaagtg ctccggtggc
     2281 tggcgcggct ggtcacgggc caggcccggg aaacggattt cgtcgcacgc tacggcggcg
     2341 aggaattcgt cgtcctcatg cccaacaccc ggggcgccga cgcttttcga ttcacccagc
     2401 ggctgtgcaa aacgatcgcc acgacgccgt ttcggtgggg ggcgaaaaaa ataaaaatca
     2461 ccctttcggc cggggtcgcc tgcttgaagg acgaagtgac cgccgaggag gatctgatcc
     2521 gccgggcgga cgaagccctc tacgccgcca aaaacggcgg ccgaaaccgg gtgtgcaact
     2581 atgacgcctg aactggccca cgctctgttg ttttgggggg ccgtgcccgt gctttgggtg
     2641 tccatccact ggcgggacgc ccgggggttc ctgatcgccg ccgtcttcgg cgtgttcgcc
     2701 gtctttcacg cctgggggga cgccggcgcc ctctgggacc tggcctttca cgggggaacg
     2761 ctcggcctgg cggccctggt gcccttttat tttcggcggt atcagagcgg actggagcgg
     2821 gaattcgccc gtcgaaaatc ccacctgtcg gaacagcgct ccaaaagcgc ccaccggcac
     2881 gacgcgatcg tgcgggagaa aaacgaactt caggacaacc tggagcgttt gcagaaccgg
     2941 ttcgccctgg tgcaggtcat ggccaccaag ctggaagcgg gcgaaatcct ccagaccctg
     3001 ggccatatgt ggaaaaaacg cacgggcgtg aaagggtgcc tgatccttcg gcggcaattg
     3061 aacggcaatt ggtccacggc cttcaccgac ggtattttta acgcccagga ttggatccgg
     3121 ttcatgaccg cccacccgtc catcgcccgt tcgcgccgga ttcgccgcta cgcggccagc
     3181 gcccgccaac tttcgtttcc cggccaacct ttcggatcga cctgcctgtt gattcccttc
     3241 acctgggaca aggacatcct ggccatgggc gtggtcgagg tggagcccca gcggatggaa
     3301 gacagcctgg aaggattcaa catcgaacgg aagttggtgt ccatcggttt g
//
LOCUS       DAOQLS010000161         3333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15393_length_3333_cov_4.839536, whole genome shotgun sequence.
ACCESSION   DAOQLS010000161 DAOQLS010000000
VERSION     DAOQLS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15393_length_3333_cov_4.839536"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="PK362_04075"
     CDS             complement(<1..93)
                     /locus_tag="PK362_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNC74047.1"
                     /translation="MLGIDPGVATTGWGVVERRGDRLVALDHGVI"
     gene            complement(124..858)
                     /locus_tag="PK362_04080"
     CDS             complement(124..858)
                     /locus_tag="PK362_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC74048.1"
                     /translation="MSGHSRWAGIKHKKGALDAKRGKVFTRIVRELTIAAKTGGGNPD
                     HNPRLRKAMDDAKAANMPMDNVKKAIQRGTGEIPGVVFEEISYEGYGPGGAAIFLTGT
                     TDNKNRTASEIRKIFSSHGGNMGESGCVAWMFHSKGLLTVDREAFKDEEKLMTAALDA
                     GAEDLKTDRSDVFEVITAPADFEKVKAAVAAAGIPVASAEVTLVPQNMIALTGGDATR
                     TIDLLDALENHDDVKDIHTNGDIEEK"
     gene            966..2624
                     /locus_tag="PK362_04085"
     CDS             966..2624
                     /locus_tag="PK362_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74049.1"
                     /translation="MRPPSGFGRRFSAGARLLLAGLLLGGSPVRGESLLARSPGPRRP
                     GTPSYRGYRIARIHIIRRRIFDTSVPSENKSVYRGINALHVSTTERTIHQQLLFEEGD
                     LYDPALARESERALRGILRLRDVSVVPVPVGNGRVDVMVESQETWSTQPSASASGTGS
                     DLKYRFGLREKNLFGLGKDLNFVYKKAAGRVSRSVGYDDPALLGTRWRLGGFYEDTTD
                     GSARSLRVERPFFSSVTPFALRTAGGYTRTETPIYVAGQEVNRLGKENREFFAGPAVS
                     LWSTTKRIRRLGVEYGYRHNKLFDTKPVRSLREDKVYHSVGPTIQWDRENFITVDHVR
                     TYDREEDINLGPTLTVNVGFARSNWFRESGDATFAKADAGRGAALGPGAFALATVSGE
                     GQREDGVWKNTRTRWAVEYNNHFSRAQTLTARATWEQVLRPGDETQLLLGGDTGLRGY
                     PLNEFAGNRLFSAIVEDRLFFFPDVLHLFGLGGVIFGDSGYAWPRGAAVRFRDLRADV
                     GVGLRFHISRASLGHVIRLDVARPLRPIEGERYWLVTFGTSQVF"
     gene            2658..3197
                     /locus_tag="PK362_04090"
     CDS             2658..3197
                     /locus_tag="PK362_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74050.1"
                     /translation="MLSHGYTILEVADTLFFMWMPPTWPATARFFLLALLVLLPVLFI
                     FRGRAAEFGARHMHFTFLTPRRIGLGAPALSLAAGLGLWLARPAPPLIQWRLDKEGVT
                     LQSPNGKSSMKWSEVVSIQFDERSPEPDKAAVILKSQDGREAWFILSWLEPPHREKLL
                     SWINSHAPVRVGAASAPTK"
BASE COUNT          544 a         1099 c         1033 g          657 t
ORIGIN      
        1 aatgaccccg tggtccaggg ccaccaggcg gtcgcctcgc cgctccacca cgccccaccc
       61 cgtcgtggcc acgcccgggt cgatgcccaa cactttcacg cgggcgttcc cgaaccggcg
      121 atcttatttt tcctcaatat cgccgttggt gtgaatgtct ttgacgtcgt cgtgattctc
      181 cagggcgtcc agaaggtcga tggtgcgcgt ggcgtcgccg ccggtgagcg cgatcatgtt
      241 ttggggcacc agggtgactt cggccgaggc gacgggaatt cccgcggcgg ccacggcggc
      301 cttcaccttt tcgaaatcgg cgggggccgt gatgacttca aaaacgtccg accgatcggt
      361 tttcaggtcc tcggcgccgg cgtccagggc ggcggtcatc aatttctcct cgtctttaaa
      421 cgcctcccgg tcgacggtga gcaggccctt ggaatgaaac atccacgcca cgcacccgga
      481 ctcgcccatg ttgccgccgt ggctggaaaa gattttccgg atttccgaag ccgtgcggtt
      541 tttgttgtcg gtggtgccgg tcaaaaagat ggccgccccg ccggggccgt agccttcgta
      601 ggagatttcc tcgaacacca ccccggggat ttccccggtg ccccgctgga tcgccttttt
      661 gacgttgtcc atgggcatgt tggccgcttt ggcgtcgtcc atggctttgc ggaggcgggg
      721 gttgtggtcg gggttgccgc cgccggtttt ggccgcgatg gtcaattccc gaacgatccg
      781 ggtgaaaact tttccgcgct tggcgtccag cgcgcctttt ttgtgtttga tgcccgccca
      841 acgggaatgg cctgacatag cgaaactcct tgagaaggtg aatgggtcgg attctaccac
      901 atttcctcgg ggggccgtcg ggcgggtccc ctggaggatc gggcgaaatc cgttataatc
      961 aagacatgcg tcccccttcg ggcttcggtc ggcggttctc ggccggggcg cgcctcctgt
     1021 tggccgggct cctcctgggc gggtccccgg tccggggcga aagcctgctg gcccggtccc
     1081 cgggtccgcg ccggccgggc acgcccagct accgcggata ccgcatcgcc cgaatccaca
     1141 tcatccgccg gcgaattttt gacacctccg tcccctccga aaacaaaagc gtctaccgcg
     1201 gcatcaacgc ccttcacgtt tcgaccaccg agcggacgat ccaccagcaa ttgcttttcg
     1261 aggaaggcga tctgtacgac cccgccctgg cccgggaatc ggagcgcgcc ctgcgcggca
     1321 tcctgcgcct tcgggacgtg agcgtcgtcc ccgtgcccgt gggcaacggc cgggtggacg
     1381 tgatggtcga atcccaggaa acctggagca cccagccctc cgccagcgcc tccggaaccg
     1441 ggagcgacct caagtaccgc ttcggcctcc gggaaaaaaa cctcttcggt ctcggaaagg
     1501 acctgaattt tgtttacaaa aaagccgcgg gacgcgtcag ccgttccgtc ggctacgacg
     1561 accccgcgct cctgggaacc cgctggcgat tgggcggttt ttacgaagac acgaccgacg
     1621 gctccgcccg gtcgcttcgg gtggagcggc cgttcttttc cagcgtcacg cccttcgccc
     1681 tgcgcaccgc cgggggctac acccggacgg aaacgccgat ctacgtcgcc ggccaggaag
     1741 tcaatcggct cggcaaggaa aaccgggaat ttttcgccgg cccggccgtg tcgctgtggt
     1801 cgaccaccaa acggattcgc cggctcggcg tggaatacgg ctaccgccac aacaaacttt
     1861 tcgacacgaa acccgtccgg agtctgcggg aggacaaggt gtaccactcg gtcggcccca
     1921 cgattcaatg ggaccgggaa aacttcatca ccgtggatca cgtgcggacc tacgaccgcg
     1981 aggaggacat caacctggga cccacgctga ccgtgaacgt gggtttcgcg cgttccaact
     2041 ggtttcggga atccggggac gccactttcg ccaaggccga cgccggccgc ggcgcggctc
     2101 tgggcccggg cgccttcgcc ctggcgacgg tttccggcga gggacaacgg gaggacgggg
     2161 tgtggaaaaa cacccgcacc cggtgggccg tcgaatacaa caaccacttc agccgcgctc
     2221 aaacgctcac ggcccgggcc acctgggaac aggtccttcg gcccggggac gaaacccaac
     2281 ttcttttggg gggcgacacg ggtctgcggg gatatccgct gaacgaattc gccggcaatc
     2341 gcctgttctc cgccatcgtg gaggaccggc tgttcttttt cccggacgtc ctgcacttgt
     2401 tcggcctggg gggcgtgatc ttcggggatt cgggctacgc ctggccccgg ggggcggcgg
     2461 tccgttttag ggatttgcgg gccgacgtcg gcgtggggct ccgatttcac atttcccggg
     2521 ccagtttggg gcacgtgatc cggctggacg tggcccggcc cctgcggccc atcgaagggg
     2581 aacgctattg gctggtgacc ttcggcacgt cccaggtgtt ttagtttggg gcgtttgcta
     2641 aactaggaag cgaccttatg ctctcccacg gttacaccat attggaagtg gcggacacgc
     2701 tcttcttcat gtggatgccg cccacctggc cggccaccgc gcggtttttc ctgttggccc
     2761 ttctcgtcct tttgccggtt ctttttattt ttcgaggtcg cgcggcggaa ttcggcgccc
     2821 ggcacatgca cttcactttt ctgacgcccc gccgcatcgg cttgggcgcc cccgccctgt
     2881 ccctggccgc ggggctcggc ctctggctcg cccgaccggc gccgcccctg atccaatggc
     2941 gcctggacaa ggagggcgtg acgcttcaaa gccccaacgg gaaatcctcg atgaaatggt
     3001 cggaggtggt ttccatccaa ttcgacgagc ggagtccgga accggacaag gcggcggtga
     3061 tcttaaaaag ccaagacggg cgggaagcct ggttcatcct ctcctggttg gaaccgcccc
     3121 accgggaaaa gcttttgagt tggatcaaca gccacgcgcc cgttcgggtg ggcgccgcgt
     3181 cggcccctac gaagtagccg acgtcccttt tttctttaat tcccccgccc aggccccgcc
     3241 gtaaaccacg ctccacgccg ccgtccaagc caaaactccc agcgccaagg gtttcccata
     3301 ggccgtcaaa tcaacgatct tttccaaata gtg
//
LOCUS       DAOQLS010000162         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15412_length_3331_cov_4.484432, whole genome shotgun sequence.
ACCESSION   DAOQLS010000162 DAOQLS010000000
VERSION     DAOQLS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15412_length_3331_cov_4.484432"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1062
                     /locus_tag="PK362_04095"
     CDS             139..1062
                     /locus_tag="PK362_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNC74051.1"
                     /translation="MVAVAQLLIGLVCAGIGGELFVRGTVGMARCMRIPPGIIAATFA
                     AFATSSPELSVAIGSALTKDPQISLGDALGSNVVNIALILGLSLFVSPPVAPLVNFNR
                     ELVVALVVPLLVGLAALDGTIARWEGWLLLSVFGGWMVVLLRGTLRERGQPSVSASRA
                     SPWEVVVFSLVGLGLLIIAGDQVVRGARALARFMGIGEFVVGATMVAIGTSVPELATT
                     LVALCRRQETIGFGIVLGSNIFNGLLIVPLTAVLCPIAVHGASTAVALVASAMAGLLL
                     VPGASGHLGRARGFLLLTLYVLFVFIISRFR"
     assembly_gap    1361..1460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1760..1849)
                     /locus_tag="PK362_04100"
     tRNA            complement(1760..1849)
                     /locus_tag="PK362_04100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1813..1815),aa:Ser,seq:cga)
     gene            complement(1912..2370)
                     /gene="smpB"
                     /locus_tag="PK362_04105"
     CDS             complement(1912..2370)
                     /gene="smpB"
                     /locus_tag="PK362_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNC74052.1"
                     /translation="MTRIAENRRAHHKYAILDTYDGGLALTGPEVKSLRAGQVNLSDG
                     FGRVENGEVFLWNVHISPYKQGSLHVTQEPTRKRKILLHRDEIRRLIGKMTTKGLTLV
                     PLELYFSKSGFAKVKLGLAKGKTAPDQREDKKRKDLQREMRRDFSGRHRV"
     gene            complement(2395..2478)
                     /locus_tag="PK362_04110"
     tRNA            complement(2395..2478)
                     /locus_tag="PK362_04110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2442..2444),aa:Leu,seq:cag)
     gene            complement(2592..3083)
                     /locus_tag="PK362_04115"
     CDS             complement(2592..3083)
                     /locus_tag="PK362_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74053.1"
                     /translation="MKITAFSDKKRWLLSLALLAFGTALLRAEPSAVNAPVLPTTTAP
                     LGRADAIETELAAGHPLAARRKAKEWARAEPRSTDAWTWVGRAFLAEGKNRRAVRAFN
                     RALKWGPHNARAFWGRGQAFEKRGRLDEAANEYRAAVLADPRLDDAQSALARLKDQST
                     LPE"
BASE COUNT          547 a          946 c         1038 g          700 t
ORIGIN      
        1 gaccaggccg gcacgccggc ggcccggcgg cgcggcgcga aaaagcattt gccgacggaa
       61 cggttcgccc tcgggtcaaa tcgggactcg cccgctggaa cttttgggcg cgccaagaat
      121 taatgataac cttcccccat ggtcgcggtt gcgcaacttc tgatcggatt ggtctgcgcg
      181 gggatcggtg gtgaattgtt cgttcgcggc accgttggaa tggcccggtg tatgcggatc
      241 cctccgggga tcatcgccgc gaccttcgcc gcttttgcga cgtccagtcc cgaattatcg
      301 gtcgccatcg ggtcggccct caccaaggac ccccaaattt ccttgggcga cgcgttgggc
      361 agcaacgtgg ttaatatcgc cctcattttg ggtctgtcgt tgttcgtctc cccgccggtg
      421 gccccgttgg tcaatttcaa ccgggaactc gtcgtggcgc tggtggtccc cctcttggtg
      481 gggctcgccg cgttggatgg aacgatcgcc cggtgggaag gatggttgtt gttgtccgtt
      541 tttgggggtt ggatggtcgt tctgttgagg ggaacgcttc gggagcgggg ccaaccttcc
      601 gtttctgcgt cgcgcgcgag cccgtgggaa gtcgttgttt tttcgttggt gggactgggt
      661 ttgcttatta ttgcgggcga ccaggtcgtg cggggagccc gggcgttggc ccgtttcatg
      721 gggatcggag aatttgtggt cggagcgacg atggtggcga tcggcacttc cgtccccgaa
      781 ttggccacca cgctcgtcgc cctctgtcgg cggcaagaaa ccatcggctt cggaatcgtt
      841 ttggggagca acatattcaa tgggttgctc atcgtccccc tcacagccgt gctttgcccc
      901 atcgcggtcc acggcgcctc aacggccgtt gccctggtcg ccagcgcgat ggcgggactc
      961 ctattggttc cgggcgcttc gggtcacctt ggccgcgcgc gcggattcct cttattaacg
     1021 ctgtatgtct tgtttgtatt cataatttcc aggttccgct gagaatccct cgccgacaga
     1081 tcggcccacc gccctttggg gcgatccgaa acgacgtcgg agggcggaga gccttccccc
     1141 taaaagcaac gaacccaacc gatggaatcg attgggctcg tccctgaagt ttgaatctta
     1201 agaatgggat gggggagtga gatttgaact cacgagcgga ggtccttggt ggcacaccaa
     1261 tgcaagatgg tggcgccgca agtgaaacgg ctccaaccgt acggtcaatg cccggagttt
     1321 gggcattcga agcttgcgcc tctaccgcat ttcgagccgc nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn cggatggatc agcgagaagc aaggtggcgg agggagtgag
     1501 atttgaactc acgagccgcg ggccttggtg gcacaccaag gcatgatggt ggcgccgcga
     1561 tggaagcggc gccaacctga cggccaatgc ccggagtttg ggcattcgaa gcttgcgcct
     1621 ctaccgcatt tcgagccgct gaccgcggag ggcgtccgcg gttaaaccgg gggagccggg
     1681 atttgaccgg cgaccgtccc cacggccatg ccccggagtg tggggcatcg tgcggcggat
     1741 ggatcagcga gaagcaaggt ggcggaggga gtgagatttg aactcacgag aagcttgcgc
     1801 ctctaccgca tttcgagtgc ggcggattca accgctctcc catccctccg taaattgata
     1861 aatcgacccc ggtttccatc ggacgtccgg ggcaggaatt cccgccggtt attataccct
     1921 gtgccgcccc gaaaaatcgc gccgcatttc ccgttgaaga tccttgcgct ttttatcttc
     1981 ccgctggtcg ggcgccgttt ttcccttggc caggcccaat ttaaccttgg caaagccgga
     2041 cttcgaaaaa taaagctcca ggggaaccaa cgtcaggccc ttggtggtca tcttgccgat
     2101 caggcggcga atctcgtccc ggtgaagaag aatttttcgc tttcgggtgg gttcttgggt
     2161 gacgtgcagc gagccctgtt tgtaggggga aatgtgcacg ttccagagga acacttcgcc
     2221 gttttccacc cggccgaacc cgtcggacaa attaacctgt ccggcccgaa gggacttgac
     2281 ctcgggcccg gtgagggcca acccgccgtc gtaggtgtcg agaatggcgt atttgtgatg
     2341 ggcccggcgg ttttcagcga tgcgcgtcat aggtggacct cacgatccgg acgctgcgcg
     2401 ggacaggact tgaacctgca cagcctttcg gccataggct tctgagacct acgtgtctgc
     2461 cagttccacc acccgcgcag cgtccggacc ttgggtctta aaatgggtgt caatcgcgcc
     2521 cacattcatg gcggtgggca agccatcgag acggttcaaa ccccggtcgg gcgttggaac
     2581 cggaattgcg tttattccgg cagcgtcgac tggtctttaa gccgggccag ggcgctttgg
     2641 gcgtcgtcca aacgcggatc ggccaggacg gcggctcgat actcgttggc ggcttcgtcc
     2701 agccgacccc gcttttcaaa agcttgtcca cggccccaaa aggcgcgcgc gttgtggggc
     2761 ccccatttga gcgcccggtt gaaggctcga accgcccggc ggttttttcc ctcggccaaa
     2821 aaggcccggc ccacccaggt ccaggcgtcc gtggagcggg gttccgcccg ggcccattcc
     2881 ttggctttcc ggcgggccgc caggggatgc cccgccgcca attcggtttc gatggcgtcc
     2941 gctcggccca ggggcgccgt cgttgtcggg agcaccgggg cattgaccgc cgatggttca
     3001 gcccgtagga gcgccgttcc aaaggccagc agggccaggg aaaggagcca acgcttttta
     3061 tcggaaaagg cggtgatctt cacgacggaa accggactat accattatgg ccgacggggt
     3121 gtcaaaacga aacaggccgg ggcggttttc cgccccggcc tgtcgtgaaa gaaacccgtt
     3181 atttcttttt ggcgcctttg cttttcggag cgggcttggc ggcggccgct tcggctttgg
     3241 ctttcaattc ttccacccgg gccgtgacgg ctttgatgga ggaattgacc cgcgtgaagt
     3301 tggcggccag gaagtcttcc ttcaaagccg t
//
LOCUS       DAOQLS010000163         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15445_length_3325_cov_4.496330, whole genome shotgun sequence.
ACCESSION   DAOQLS010000163 DAOQLS010000000
VERSION     DAOQLS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15445_length_3325_cov_4.496330"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PK362_04120"
     CDS             <1..297
                     /locus_tag="PK362_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sphX"
                     /protein_id="HNC74054.1"
                     /translation="VLPSEQTVMDGTYQPLARPIFIYPSKRSAKKPEVQAFVEFYLKN
                     APALVKEVKYVPLPNEEYAGVLARFQTGQTGTAFGGKAEIGVKIQDLLKRKPKI"
     gene            437..1438
                     /gene="pstC"
                     /locus_tag="PK362_04125"
     CDS             437..1438
                     /gene="pstC"
                     /locus_tag="PK362_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNC74055.1"
                     /translation="MATKTKSPSAPPRALSAPSARPEAGPVAPPRFPALPRARVGTGR
                     RWEERIVRAVLFFAAFSSVLITVAIVGILLAESLSFFRHVSLWTFLTDTQWTPLFDDA
                     HYGILPLVGGTLVVAAVALAFALPVGTITAVYLSEYAPHRVREVVKPFLELLTAVPTV
                     VFGYFALLFVTPLLQHVFKDLPGFNMLSAGLVIGVMIIPYVSSLSEDAMRAVPMILRE
                     ASYAMGATRFQTSVRVVVPAAFSGIASAFVLGFSRAIGETMVVAIAAGMQPNLTLNPL
                     QPAATITAYIVQVALGDLPHGSIGYQTIFAAGLTLMSMTLVFNIAGHVLRKRYRESY"
     gene            1441..2349
                     /gene="pstA"
                     /locus_tag="PK362_04130"
     CDS             1441..2349
                     /gene="pstA"
                     /locus_tag="PK362_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC74056.1"
                     /translation="MRDLKADIAQRKRHDRWFAVLGLLATSLGIVALLALLIDLALDG
                     AGRLSFSFFANFPSRFPEKAGVLSAWVGTAVVMLVTGLAAVPLGVAAGLYLEEYAPKN
                     RITTLIEINIANLAGVPSIVYGLMALGVFVYGLHLGQSVLTAGLTLALLILPIVIMAT
                     REAIRTIPASIREAAFAVGATKWQMVRHHILPYSAGGILTGVIIGLSRAIGESAPLIT
                     VGALTFIAFLPPSPVVGKFPFLSLEWLTSPYTVLPIQLFNWVSRPQKAFHLNAAAAGL
                     ILMGMTLAMNAAAIAIRYRFRKRIKW"
     gene            2343..3137
                     /gene="pstB"
                     /locus_tag="PK362_04135"
     CDS             2343..3137
                     /gene="pstB"
                     /locus_tag="PK362_04135"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC74057.1"
                     /translation="MVETTTSPRAGLAEKARVEKLSFYYGTHRVLKNIDLTIPEKKVT
                     ALIGPSGCGKTTLLRCFNRMHDLYPGNRYEGQIRLFPENTDILAPAVDPIEVRMRISM
                     VFQKANPFPKTIFENVAYGLRVRGGMPKSEIAGRVEAALRGAALWDEVKDRLHTLGAN
                     LSGGQQQRLCIARALVTDPEIILFDEPTSALDPIATGKVEELVADLKNRVTILIVTHN
                     MQQAARVSDFTGFMYLGELAEFDVTDKVFTNPSNKLTEDYITGRFG"
     gene            3143..>3325
                     /locus_tag="PK362_04140"
     CDS             3143..>3325
                     /locus_tag="PK362_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNC74058.1"
                     /translation="MERHFDEDLRDLEKRVLEMGALCESMIHKAVKALIDRDGELTQE
                     VFRMEVEANRRHVEIDE"
BASE COUNT          601 a         1136 c          939 g          649 t
ORIGIN      
        1 gtgttgccct ccgaacaaac cgtcatggac ggcacgtacc aacccctggc gcggcccatc
       61 ttcatctacc cgtcgaagcg ctccgccaag aaacccgaag tccaggcctt tgtggagttt
      121 tatcttaaga acgccccggc gctggtcaag gaggtcaaat acgttcccct gcccaacgag
      181 gaatacgcgg gcgtgctggc ccgattccaa accggccaaa ccgggaccgc cttcggcggc
      241 aaagccgaaa tcggggtcaa aatccaggac ctgctgaaac ggaaacccaa gatctaaaat
      301 ccgcgatccg ccccgaccgg aaggtcggga cgggtccgga ccgtggctct ctcccgccga
      361 catcacgatt cccagggagc cacggtccgg gcagccaaaa atgagaacac aaacgcttca
      421 gacattcgcc ccgttgttgg cgaccaaaac aaaatccccc tccgcgccgc cacgagcgct
      481 ttcggctcca tcggcccggc ccgaagccgg gcccgtcgcc ccgccgcgat tcccggcgct
      541 tccccgggcc cgtgtcggga ccggacgacg gtgggaggag cggatcgttc gcgccgttct
      601 tttcttcgcc gccttttcct ccgtcctcat caccgtggcc atcgtcggca tcctcctggc
      661 cgagtccctc tcctttttcc ggcacgtttc cctttggact tttctgaccg acacccagtg
      721 gacgccgctt ttcgacgacg cccactacgg cattctcccc ctggtgggcg gaacgctggt
      781 ggtggccgcc gtggccctgg ccttcgccct gccggtgggg accatcaccg ccgtctactt
      841 gagcgagtac gccccccacc gcgtgcggga ggtggtcaaa ccctttctcg agcttttgac
      901 cgccgttccc acggtggttt tcggttactt cgccctgttg ttcgtgacgc cgctcctgca
      961 acacgttttc aaggatttgc cgggattcaa catgctctcg gcgggcctgg tgatcggggt
     1021 catgatcatc ccctacgtca gttcgctctc ggaggacgcc atgcgggcgg tccccatgat
     1081 tctccgggag gccagctacg ccatgggggc cacgcgcttt caaacctcgg tccgggtggt
     1141 ggtgccggcg gccttctcgg gcatcgcctc ggccttcgtg ctggggtttt cccgagccat
     1201 cggcgaaacc atggtcgtgg ccatcgccgc cgggatgcag ccgaacctga ccctgaaccc
     1261 cctccagccc gccgccacca tcacggccta catcgtccag gtcgccctcg gggatttgcc
     1321 ccacgggtcc atcggctacc agaccatttt cgccgcggga ttgaccctga tgtcgatgac
     1381 cctcgtcttc aatatcgcgg gccacgtcct tcgcaaacgc tatcgggagt cctactgacc
     1441 gtgcgcgact tgaaagcgga catcgcccag cggaaacggc acgaccggtg gttcgcggtc
     1501 ctcggtcttt tggccacgag tctcggcatc gtcgccctgc tggccttgtt gatcgacctg
     1561 gccctggacg gagcgggccg cctctctttt tcgtttttcg cgaattttcc ttcccgtttc
     1621 cccgaaaaag ccggcgtgct atcggcatgg gtcggcacgg ccgtggtcat gctcgtcacc
     1681 ggcctggcgg ccgtgcctct gggcgtggcg gcgggccttt acttggagga atacgccccg
     1741 aaaaaccgaa tcacgacttt gatcgaaatc aacatcgcca atttggccgg cgtcccttcc
     1801 atcgtctacg gtctcatggc cctgggtgtc ttcgtttacg gcctacatct gggccaaagc
     1861 gtgttgacgg cggggttgac cctggccctg ctcatcctgc ccatcgtgat catggccacc
     1921 cgggaggcga tccgcaccat tccggcctcc attcgggaag ccgccttcgc cgtgggcgcg
     1981 accaagtggc agatggtgcg ccaccacatt ctgccctact ccgccggcgg catcttgacg
     2041 ggggtgatca tcggtctttc ccgggccatc ggcgaaagcg cgcccctcat caccgtgggg
     2101 gccttgacct tcatcgcctt tcttccgccc tcccccgtgg tgggaaaatt tccctttttg
     2161 tccttggagt ggctcacctc gccctacacc gtcctgccca tccaactttt caactgggtg
     2221 tcgcggcctc aaaaagcgtt ccacctgaac gcggccgccg cgggcctgat tttgatgggg
     2281 atgactctgg ccatgaacgc cgcggccatc gccatccgat accgtttccg aaagaggatc
     2341 aaatggtaga aacgacgacg tcgccccggg ccgggttggc cgaaaaagcg cgggtggaaa
     2401 aactgagctt ttactacggg acccaccggg tgcttaaaaa catcgacttg acgatccccg
     2461 aaaaaaaggt gaccgccttg atcggcccgt cggggtgcgg caagacgacc ctgttgcgtt
     2521 gcttcaaccg catgcacgac ctttacccgg ggaaccgcta cgaggggcaa attcgccttt
     2581 tcccggagaa caccgacatc ctggcccccg cggtcgaccc catcgaagtc cgcatgcgga
     2641 tcagcatggt ctttcaaaaa gccaacccgt tccccaaaac aatcttcgaa aacgtggcct
     2701 acggccttcg ggtccggggc ggcatgccca aatccgaaat cgccggccgg gtggaggccg
     2761 ccctgcgggg cgcggccctc tgggacgagg tcaaagaccg ccttcacacc ctcggcgcca
     2821 atttgtccgg cggccaacaa caacgccttt gcatcgcccg ggccctggtg accgaccccg
     2881 agatcattct gttcgacgaa cccacctccg ccctggaccc catcgccacg gggaaagtgg
     2941 aggaactggt ggccgacctc aaaaaccgcg tgaccatcct gatcgtcacc cacaacatgc
     3001 aacaagccgc ccgggtgtcg gacttcaccg gcttcatgta cctcggcgaa ctggccgagt
     3061 tcgacgtgac ggacaaagtg ttcacgaacc cctcgaacaa gctcaccgag gattacatca
     3121 cgggaaggtt tgggtgaccc ccatggaacg gcacttcgac gaagacttgc gggatttgga
     3181 gaaacgcgtc ctggaaatgg gcgccctctg cgaatcgatg attcacaaag ccgtcaaagc
     3241 cctcattgat cgggacgggg aattgaccca agaggttttt cggatggaag tcgaggccaa
     3301 ccgacgccac gtcgaaatcg acgag
//
LOCUS       DAOQLS010000164         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15520_length_3315_cov_5.244172, whole genome shotgun sequence.
ACCESSION   DAOQLS010000164 DAOQLS010000000
VERSION     DAOQLS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15520_length_3315_cov_5.244172"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /gene="alr"
                     /locus_tag="PK362_04145"
     CDS             complement(<1..1158)
                     /gene="alr"
                     /locus_tag="PK362_04145"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNC74059.1"
                     /translation="MRRPSRGAPAALPALPSYAAGSLRPTWAEIDLNAFRHNLKALAR
                     FLPRRVSIMAVLKADGYGHGALPLARAAARLGKKVRLWGFGVSSVEEGLALRAGGLRE
                     RILVLGSLYPFESLDVALAGRLTPTIGSRGAAQALASRARRLGRPAECHVKIDTGMGR
                     IGMAPATAREALGLFQANPFLRVEGVYTHLACADSAPETAQQLRAFEEALGGLSTRPP
                     WVHAANSAGALGRPAARYDLVRPGIALFGVPPSPSLGKILSLRPVLSWKTRVVFVKMI
                     PKGTPVSYGWTWRAPRRSRIATLPVGYADGYPRVLSNRGEVLLRGRRCPVVGRVTMDQ
                     ILVDVTGLRGVDVGEEVVLIGQQGGQKITAEDLALAAGTIPYEILCGISKRV"
     gene            complement(1158..2537)
                     /gene="dnaB"
                     /locus_tag="PK362_04150"
     CDS             complement(1158..2537)
                     /gene="dnaB"
                     /locus_tag="PK362_04150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNC74060.1"
                     /translation="MPESIREVLGLGKVPPQNTEAEMAVLGSMLIEKEAALKAFDMLR
                     EGDFYSPTHQIVFEAIRTLHDQNVAADIVTVGDYLQHNNKLNESVSRATLIQLTNLVP
                     SALNVEHYARIVREKSILRSMIDIAKKVSGNCFESGDEAQKLLDEAQQMFFELAQARS
                     TKGMTAASVLMQGAISTLEKMAESSKLVTGVATGFSELDKMTSGLQAANLVIIAGRPS
                     MGKTAFCLNIAEHVALKEKRPVVFFSLEMSEQEIGLRLLSSQARINLRSVREGFLSRK
                     SWPNITNVASQIAGAPLYFDFSTSPSILDIRSSARRWAHELRHRGNPLSLIVIDYIQL
                     LHAGGAVESRQQEISEISRSIKALARELQVPVIALSQLNRRPEDKGREGRPQLSDLRE
                     SGALEQDADVVAAIFREEVYRRDDPDLKGKAKLFVLKQRNGPIGDIDLNFLSDYTKFV
                     DPAREGEEF"
     gene            complement(2563..3015)
                     /gene="rplI"
                     /locus_tag="PK362_04155"
     CDS             complement(2563..3015)
                     /gene="rplI"
                     /locus_tag="PK362_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNC74061.1"
                     /translation="MKTKVILQKDIPNLGIAGDAKDVAPGYARNYLIPRKLAVPSTPR
                     AREYWETQKEKVEAERESKLVASQERAKQIQDVVLTLSSRAGQDGKLYGSITTGDVAR
                     AFAEKGVTLDRRWIELKDQIRTTGEFQGAVRLHPQVRATFKIQVQPQA"
     gene            complement(3017..3211)
                     /gene="rpsR"
                     /locus_tag="PK362_04160"
     CDS             complement(3017..3211)
                     /gene="rpsR"
                     /locus_tag="PK362_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNC74062.1"
                     /translation="MCRFCAEKISEVDYKAIPILRGFLTARGKILSGRTTGNCAGHQR
                     QLTQAIKRAQTLALLPYTGE"
BASE COUNT          566 a         1007 c         1147 g          595 t
ORIGIN      
        1 aacccgtttg gagatgccgc aaaggatctc gtaggggatc gtcccggcgg cgagcgccag
       61 atcctccgcc gttatttttt gcccgccctg ttggccgatc aaaacgacct cctcgcccac
      121 gtccacgccg cgcaggccgg tgacgtccac caaaatttgg tccatggtca cccgccccac
      181 caccgggcat cgccggcccc ggaggaggac ctccccccgg ttggagagga cccgggggta
      241 gccgtcggcg taacccaccg gcagggtggc gattcgggac cgccggggcg cccgccaggt
      301 ccagccgtag ctgaccgggg tgcccttggg aatcattttc acaaaaacga cgcgggtctt
      361 ccaggacaaa acggggcgaa gggaaaggat tttccccagg gagggcgacg ggggcacgcc
      421 gaagagcgcg atccccggcc ggaccaaatc gtagcgggcg gcgggccggc ccagggcccc
      481 cgcggaattg gcggcgtgga cccagggggg gcgggtcgaa aggccgccga gcgcctcttc
      541 aaaggcgcgc aactgttggg ccgtttcggg ggcggaatcc gcgcaggcca aatgggtgta
      601 gacgccttcc acccgcaaaa acgggttcgc ttggaaaaga ccgagggctt cccgggccgt
      661 ggccggagcc atgccgatcc gccccatgcc cgtgtcgatt tttacgtggc attccgcggg
      721 gcggccgagc cgccgggccc gggaggccaa ggcctgggcg gcgccgcggc ttccgatcgt
      781 gggcgtcaag cggccggcca gggccacgtc caaactctcg aagggataaa ggctccccag
      841 aaccaagatc cgctcgcgaa gccctccggc ccgaagggcc aagccctctt cgacgctgga
      901 aacaccgaac ccccagaggc gaactttctt tcccaggcgc gcggccgccc gggccagggg
      961 aagggccccg tggccgtagc cgtccgcttt gaggacggcc atgatcgata cgcgccgggg
     1021 caaaaaacgc gccagggctt taagattgtg acggaaggcg ttgagatcaa tttccgccca
     1081 ggtcggacgg agggaaccgg cggcgtagga cggaagggcg ggcagcgccg cgggggcgcc
     1141 ccgcgacgga cgacgcacta gaactcctcg ccctcgcggg cgggatcgac gaacttggtg
     1201 taatcggaaa ggaaattcaa atcgatgtcg ccgatggggc cgttgcgctg tttgagcacg
     1261 aacaatttgg ctttcccctt aaggtccgga tcgtcccgcc ggtagacttc ctcccggaaa
     1321 atggcggcga cgacgtcggc gtcctgctcc agggccccgg attcccgcaa atcggacaat
     1381 tggggccggc cttcgcgacc cttgtcctcg ggacgccgat tgagctggga gagggcgatc
     1441 acgggaactt gcaattcccg tgccaaggcc ttgatggacc gggaaatttc cgagatttcc
     1501 tgctgccggc tttcgacggc tccgccggcg tggagcagtt gaatgtagtc gatgacgatc
     1561 aaggacaggg ggttgccccg gtggcgcagc tcgtgggccc aacggcgggc ggaactgcgg
     1621 atgtccaaaa tggacgggct ggtggaaaaa tcgaaataga gcggggcccc ggcgatttgg
     1681 ctggccacgt tggtgatgtt gggccagctt ttccgggaca aaaagccctc ccgcacgctc
     1741 cgcaaattga tgcgggcctg gctggaaagc agccggagcc cgatttcctg ctcgctcatt
     1801 tccagggaga agaacaccac cggccgcttt tccttcaagg ccacgtgctc ggcgatgttc
     1861 aggcagaagg cggttttgcc catggagggc cggccggcga tgatgaccag gttggcggcc
     1921 tgaaggcccg aggtcatctt gtccagctcc gaaaaaccgg tggccacgcc cgtgaccagc
     1981 ttgctggact cggccatctt ctccagggtc gaaatggcgc cctgcatgag gacgctggcg
     2041 gcggtcatgc ctttggtgga acgcgcctgg gccagttcaa agaacatctg ctgggcttcg
     2101 tccaacaact tctgggcttc gtccccggac tcgaagcaat tgcccgagac ctttttggcg
     2161 atgtcgatca tgctgcggag aatggatttt tcccggacga tgcgggcgta gtgctcgacg
     2221 ttgagggcgg aggggacgag gttggtcagc tggatcaggg tcgcccggct gacggactcg
     2281 ttcagcttgt tgttgtgctg gaggtaatcg ccgacggtga cgatgtcggc ggccacgttt
     2341 tggtcgtgga gggtccgaat ggcctcgaag acgatttggt gggtggggga atagaaatcc
     2401 ccctcccgaa gcatgtcgaa ggccttgagg gcggcctctt tttcgatcaa catgctcccc
     2461 aggacggcca tttcggcctc cgtgttttgg ggagggactt tgcccaaacc caggacttcc
     2521 cgaatggatt ccggcatggg acctccgggc gcccgggcgg acttacgcct ggggttggac
     2581 ctggatcttg aaggtcgcgc gaacctgcgg gtgcaggcga acggcgccct ggaattcgcc
     2641 cgtggtgcgg atctggtctt tgagctcgat ccaacggcgg tccagggtca cgcccttttc
     2701 ggcgaaggcc cgggccacgt cgcccgtggt gatggagccg tagagcttcc cgtcctggcc
     2761 ggcgcgggaa ctcaaggtga ggaccacgtc ctgaatctgt ttcgcccgtt cctgggacgc
     2821 gaccaatttg gactcgcgct cggcctccac tttttccttc tgggtttccc agtattcccg
     2881 ggcccggggg gtggagggca ccgcgagctt ccgcggaatc aggtagttcc gggcgtagcc
     2941 gggggcgacg tcttttgcgt cgccggcgat gcccaggttc ggaatgtctt tttggagaat
     3001 gactttcgtt ttcatgttat tcccccgtgt agggcaggag cgccagggtc tgggcgcgct
     3061 tgatggcctg cgtcaattga cgctggtggc cggcgcagtt gcccgtggtg cgtcccgaaa
     3121 ggattttgcc ccgggcggtc aaaaagcccc ggagaatggg aatggctttg taatccacct
     3181 cggaaatctt ttccgcgcag aaacggcaca ctttgcggcg cgggggcgga cggcggccgc
     3241 ggccgccggg gccggtggga cgcccgccgg gacccgagcg ggcgggccgt cgtgctggtg
     3301 cccgccgcat ttgcc
//
LOCUS       DAOQLS010000165         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15569_length_3309_cov_3.680086, whole genome shotgun sequence.
ACCESSION   DAOQLS010000165 DAOQLS010000000
VERSION     DAOQLS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15569_length_3309_cov_3.680086"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PK362_04165"
     CDS             complement(<1..977)
                     /locus_tag="PK362_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC74063.1"
                     /translation="MPLINWKASPGDRIPAGNQNRPRYLVDGQVRSWDGPSFEVKSPV
                     FEEGPGGGVPRSLGRCGLLDGPTGLSALEAAVRAYDKGRGAWPTSTVATRVAALRNFL
                     GRMKGRRLEIARLICWEVGKPWADSLAEVDRTVIYGEQTAAALEELDQQGRDYLVSDG
                     FRARVRRSPIGVGLCMGPYNYPLNETFATLLPALVMGNPVVVKLPRLGMLCTMPLLED
                     FAASFPPGVVNIISGEGATVVTPIVASGRVDLLAFIGSAKTAGQLKHAHPKPNRLRSV
                     LGLGAKNAAIVLPDADLDLAVKEGVAGALTFNGQRCTALKIFFVHKSIA"
     gene            complement(993..3308)
                     /locus_tag="PK362_04170"
     CDS             complement(993..3308)
                     /locus_tag="PK362_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNC74064.1"
                     /translation="MTAPALLITQCLQVDFVKPIGKHEPLPNKLHVGADEARRLLGDR
                     PEDGPLARTVRWAQEQGPDRLGVIHIRDWHNPDDPSQQDHLRQFGAHCLRNTPGAGFV
                     FPAPPANRPDIAVVDSLTLNDFNGTTLQGRLAPWAGQPTRVGLLGVWTEAKITYLAYE
                     LKTRYPTFRIGVCSALTASSSRAHHFMALDQLQRLLGVEIFSSVGAFVRFLGGRDEDW
                     PWESPADRGAASFTGVDNLSATDRDLLKYLFRDSRRADFKVLDGGYSGNVVLAAESED
                     LHGHRQAPHVVKIGPQVPMGQEREAFEKVESVLGNNAPHIDEFADFGDRGAIKYRYAA
                     MGGGFSTTFQKKYAAGLAPEKTRYYLNQVFREQLGRFYRAAELEKCNLLSYYQYDLPG
                     LGARIRPRVEAVYGAPAAGDALRFSNGVSFYNPCRFYDDDLPTLLPLADASARWSYVH
                     GDLNGANILIDGQDNVWLIDFFHTHRGHVLRDLAKFENDLLYIFTEVKSPAEFDQALR
                     LTDHLMAVEDLARPPADLPADITHPPLRRTFETLRVLRSFYPELIQAHRDPLQLHIAQ
                     LRYAAHTLSFDESNDFQKRWALYTGARCAERIKTSLLRTGPLRIDWIDPRWTASGALG
                     LTILPGRRDVGRSLADDVAALKSQGVTDVVCLVPEDELRRYGVPGLLAEYRKAGLRVH
                     HWPVLDQKTTSPAEMEKIVADVGGRLDAGARVAVHCVGGLGRSGLVAACFLKSRGAST
                     DEAIVEVRRVRSPRAVESAVQEQFVTNFHPR"
BASE COUNT          601 a         1072 c         1090 g          546 t
ORIGIN      
        1 tccgcgatgg atttgtggac gaaaaagatt ttaagcgccg tgcaacgttg tccgttgaag
       61 gtgagggccc ccgccacgcc ttccttgacc gccaggtcga gatcggcgtc gggcagcacg
      121 atggcggcgt ttttggcccc caggcccaac accgagcgga gccggttggg tttcgggtgg
      181 gcgtgcttca actgccccgc cgtcttggcc gagccgatga aggccagcaa atccacccgg
      241 cccgaggcca cgatgggggt caccaccgtc gccccttccc cggaaatgat gttgaccacc
      301 cccggcggga aactggcggc gaaatcctcg agcaggggca tggtgcagag catccccagc
      361 cggggcaatt tgaccaccac gggattcccc atgaccaggg cgggcaacag cgtggcgaag
      421 gtttcgttca gcggataatt gtacgggccc atgcagagcc ccacgccgat gggcgatcgc
      481 cgcacccggg cccgaaaccc gtcgctcacc aaatagtccc ggccctgctg gtccaattcc
      541 tccagggcgg cggcggtctg ttcgccgtag atcaccgtcc ggtccacctc ggccagggaa
      601 tcggcccagg gtttgcccac ttcccaacaa atcaaccggg cgatttccaa ccggcgcccc
      661 ttcatccggc ccaggaaatt ccgcagggcg gccacgcgcg tggccacggt ggacgtgggc
      721 caggcgcccc ggcccttgtc gtaggcccga accgccgcct ccagagccga caaacccgtc
      781 gggccgtcca aaagtccgca tcgtcccagg gaccggggga ccccaccgcc cggaccttcc
      841 tcgaacaccg gcgacttcac ttcaaaagac ggcccgtccc aggaacgaac ttggccgtcc
      901 accaagtacc gggggcggtt ttggtttccg gcgggaatcc ggtctccggg cgacgctttc
      961 cagttgatca ggggcatggg atttcctttg gcctatcggg gatgaaaatt cgtcacgaac
     1021 tgttcctgca ccgccgactc caccgcccgg ggggaccgaa cccggcggac ttccacgatc
     1081 gcttcgtcgg tcgaggcgcc ccgggatttt agaaagcagg ccgccacgag acccgaccgc
     1141 cccaggcccc cgacgcaatg gaccgccacc cgggcgccgg cgtccagccg tccgccgacg
     1201 tcggccacga ttttttccat ttccgccggc gacgtcgttt tttggtcgag caccggccaa
     1261 tgatggaccc gaagccccgc cttccggtat tcggccagca agcccggcac gccgtaacgc
     1321 cgcaattcgt cctcgggcac caggcacacc acgtcggtca ccccctggct tttcagagcg
     1381 gccacgtcgt cggccaggga acggcccacg tcccggcggc ccggcaggat cgtgaggccc
     1441 agggcgccgg aggccgtcca acgggggtcg atccaatcaa tccgcagagg ccccgtgcgg
     1501 aggagggacg ttttgatccg ctccgcgcaa cgggccccgg tgtagagggc ccagcgtttt
     1561 tgaaaatcgt tggactcgtc gaagctcagg gtgtgggccg cgtagcgcag ttgggcgatg
     1621 tgcaactgca gggggtcccg gtgggcttgg atcaactcgg gataaaagga acggagaacc
     1681 cgaagggtct cgaaggtccg ccgcaggggc gggtgggtga tgtcggcggg caaatccgcc
     1741 gggggccggg ccaaatcctc caccgccatc aaatggtcgg tcaggcgcag ggcctggtcg
     1801 aattccgccg gggacttcac ctcggtgaaa atatacagca ggtcgttttc gaacttcgcc
     1861 aaatcccgca ggacgtggcc ccggtgggtg tggaagaaat cgatgagcca cacgttgtcc
     1921 tggccgtcga tcaggatgtt ggccccgttc aaatccccgt ggacgtagga ccaccgggcc
     1981 gaggcgtcgg ccaggggcag gagggtcggc aaatcgtcgt cgtagaaccg gcaggggttg
     2041 taaaaggaaa ccccgttgga aaaacgcaag gcgtcccccg ccgccggggc gccgtagacc
     2101 gcctccaccc gggggcgaat gcgggcgccc aggcccggaa ggtcgtattg atagtaagac
     2161 aacaaattgc atttttccaa ttccgccgcc cggtagaagc ggcccagctg ttcccggaac
     2221 acttgattca ggtaataacg ggttttctcg ggcgccaggc ccgcggcgta ttttttttgg
     2281 aaggtggtgg agaacccgcc gcccatggcc gcgtagcggt atttgatggc gccccggtcg
     2341 ccgaaatcgg cgaactcgtc gatgtggggc gcgttgttcc ccagcacgga ctcgactttt
     2401 tcaaaggcct cccgctcctg gcccatggga acctgggggc cgattttgac cacgtgggga
     2461 gcctggcggt gcccgtgcag gtcctcgctt tcggcggcca gcacgacgtt ccccgaatag
     2521 ccgccgtcca acactttgaa atccgcccgg cgggaatccc gaaagagata cttcaaaagg
     2581 tcccggtccg tggccgaaag gttgtccacg ccggtgaagg aggcagcgcc ccggtccgcc
     2641 ggggattccc agggccaatc ctcgtcccgc ccgcccaaaa agcgcacgaa ggcccccacg
     2701 gacgaaaaaa tctccacccc caacaggcgt tgaagctggt ccagggccat gaagtgatgg
     2761 gcccgggagg agctggccgt cagggccgag caaacaccga tgcggaaagt tggatagcgg
     2821 gttttaagtt cgtaggccag gtaggtgatt ttggcttcgg tccaaacgcc caggagaccc
     2881 acccgcgtcg gctgtcccgc ccagggcgcc aaacggccct ggagcgtcgt gccgttgaaa
     2941 tcgttcagcg tgagggaatc caccaccgcg atgtcgggtc ggttcgcggg cggcgcgggg
     3001 aaaacgaaac ccgcaccggg ggtgtttcgc aggcagtggg cgccgaattg ccgcagatga
     3061 tcctgttggg agggatcgtc ggggttgtgc cagtcccgga tgtggatgac gcccagtcgg
     3121 tcgggcccct gttcctgggc ccagcggacc gtgcgggcca gagggccgtc ctcgggacgg
     3181 tcccccaaaa gacgccgggc ctcgtcggcg cccacgtgca gtttgttggg caggggttcg
     3241 tgcttgccga tgggtttgac gaaatccacc tggaggcact gggtgatgag caaagccggg
     3301 gccgtcatg
//
LOCUS       DAOQLS010000166         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15592_length_3305_cov_4.235385, whole genome shotgun sequence.
ACCESSION   DAOQLS010000166 DAOQLS010000000
VERSION     DAOQLS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15592_length_3305_cov_4.235385"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK362_04175"
     CDS             complement(<1..405)
                     /locus_tag="PK362_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNC74065.1"
                     /translation="MLGHRCVCVHGHFYQPPRENPWLEDIEVQDSAHPYHDWNERITR
                     ECYAPNAASRVLDGEGRLVELIDNYRRISFNFGPTLLSWMERHAPEAYEGIRAADRAS
                     VRERSGHGNAWAQVYNHAIMPLASRRDKETQVV"
     gene            complement(419..1651)
                     /gene="glgC"
                     /locus_tag="PK362_04180"
     CDS             complement(419..1651)
                     /gene="glgC"
                     /locus_tag="PK362_04180"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNC74066.1"
                     /translation="MIRSKVLGIVMAGGKGERLKPLTIERGKPAVPFGGKYRIVDFVL
                     SNFVNSGIFSVYVLVQYMSQSLIDYLRVGWSNRGITPDHFITVVPPQMRMGELWYRGT
                     ADAVSQNLNLIRDFDPDYVAVFGADHIYRMDISQMLAYHVEKQAHVTISALPVPLAEA
                     KGFGILGVSKSGRLTDFEEKPKNPKPMPGNPEMAYSSMGNYIFNRDVLEEVLTDDIEK
                     DSAHDFGKNILPTLLEKHRVYAYNFQAAPLPGQKDYEEAGYWRDVGNLESYWSSHMDL
                     LGEKPRLDLNNDEWPISAGRYNGPPARILGRQVEDSILSEGVVVNGAFVKHSVIGRGV
                     VIHPGAVIEDSVLMDFCEVGPNCRFRRVVADRLNVFPPDTVIGHDAEKDQSAGYHVDK
                     SGIVSIARGRSKWAHLKR"
     gene            complement(1648..2328)
                     /locus_tag="PK362_04185"
     CDS             complement(1648..2328)
                     /locus_tag="PK362_04185"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNC74067.1"
                     /translation="MGLRILWENEDYWAVDKPAGQEVIPGRGPATSPCLREELARAAG
                     RTVWVVHRLDRDTSGVLVLAKNADAHRSLSLDFENRRVEKEYLAAVRGPMNVPAGLFR
                     DPLRAFGSGRMGVDPDGKPSETQFETLETWTTGALLRVRPRTGRRHQIRVHLHHGGHP
                     VLGDRLYGPPPRPIGGAPRLLLHAARIAFRSPAGEPIAVECPLPADFRDALRIVGAGE
                     ISYLKNQS"
     gene            complement(2332..>3305)
                     /locus_tag="PK362_04190"
     CDS             complement(2332..>3305)
                     /locus_tag="PK362_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC74068.1"
                     /translation="ELILTDLDMPGATNGTELTKRARAQTNADVIIMTGYPDLTSAIH
                     AVREGAYDYLVKPFSPDTLKMAIDRCAAKRHLSKELEREKTLRAELNRAYIELSQMQK
                     VRDLFGQFTTPEVARFVMEHPDDFWKRGERRDVTVLFADVRGFTPYARMVSPEKAVES
                     LNQIFDVLVGAIQDQGGVVNKFLGDGVMGVFGAPQKLEDHAAAAARAATKARALLRFA
                     MGQTPEADGLHVGFALNTGEVLAGCLGARGRSEYSVIGSAVNLAARIEKVAGPGEIVL
                     GPDTAQRVAAFFHLERRPAVTLAGFPDPVDLSLLGEPKPGAFVSAPR"
BASE COUNT          598 a          982 c         1094 g          631 t
ORIGIN      
        1 gacgacctgg gtttccttgt cccgccggga ggccaagggc atgatggcgt ggttgtacac
       61 ctgggcccag gcgttcccgt ggccggaccg ttcgcgaacg ctggcgcggt cggccgcccg
      121 gattccttcg taggcttccg gggcgtggcg ctccatccag ctcaacaggg tggggccgaa
      181 gttaaaggaa atgcggcggt aattgtcgat gagttcgacg aggcgcccct cgccgtccaa
      241 gacgcgggag gccgcgttcg gcgcgtaaca ttcccgggtg atccgttcgt tccagtcgtg
      301 atacgggtgg gcggaatcct ggacttcgat gtcttccagc caggggtttt cccggggcgg
      361 ttggtaaaaa tgtccgtgaa cgcaaacgca ccgatggccg agcatgggaa acggggggtc
      421 agcgcttcaa gtgcgcccac ttgctgcggc cgcgggcgat cgagacgatg ccggatttgt
      481 cgacgtggta accggcggat tggtctttct ccgcgtcgtg accgatgacg gtatcgggcg
      541 ggaaaacgtt gaggcggtcg gccaccaccc ggcggaaccg gcaattgggg cccacttcgc
      601 aaaaatccat gaggacgctg tcttcgatga cggccccggg gtggatgacg acgccccggc
      661 cgatcacgct gtgtttcacg aaggcgccgt tgaccaccac gccttcgctc aggatggaat
      721 cctccacctg gcgccccaaa atgcgcgccg gcggcccgtt gtaacgcccc gcggaaatcg
      781 gccattcgtc gttgttcaaa tccaaccggg gtttttcccc cagaaggtcc atgtgcgacg
      841 accaatagga ttccaggttg ccgacgtcgc gccaataacc ggcctcttcg taatcctttt
      901 ggcccggaag gggcgcggct tggaaattgt aggcgtaaac gcggtgtttt tcgagcaagg
      961 tgggcaggat gtttttgccg aaatcgtggg ccgagtcctt ttcaatgtcg tcggtgagga
     1021 cttcttcgag gacgtcccgg ttgaaaatgt agttccccat ggacgaatag gccatctcgg
     1081 gatttcccgg catgggtttc ggatttttgg gcttttcctc aaaatccgtc aggcggccgg
     1141 atttgctgac ccccaaaatg ccgaatccct tggcttcggc caagggcacc gggagggcgg
     1201 aaatcgtcac gtgggcttgt ttttccacgt ggtaggccaa catttggctg atgtccattc
     1261 ggtaaatatg gtccgcgccg aacacggcca cgtagtcggg atcgaaatcc cggatcaagt
     1321 tgaggttttg cgacaccgcg tccgccgtgc cgcgatacca caattccccc atgcgcattt
     1381 ggggcggaac cacggtgatg aaatggtccg gggtgatgcc ccggttcgac cagccgaccc
     1441 gcaggtaatc gatcaaggat tgggacatgt attggacgag cacgtagacg gaaaagatgc
     1501 cggagttcac aaaattggaa aggacaaagt cgactatgcg gtatttcccg ccgaagggaa
     1561 cggcgggttt tccccgttcg atggtcaagg gtttgaggcg ttcgcctttt ccccccgcca
     1621 tgacgatgcc caacactttg gagcgtatca tgattggttt ttaagatagc taatttcgcc
     1681 cgcgcccaca atccgaaggg cgtcccgaaa atcggcgggg agcgggcatt ccacggcaat
     1741 gggttccccc gcgggcgatc gaaaagcgat gcgggcggcg tgcaaaagga ggcggggggc
     1801 gcccccgatg ggccgcgggg gcggcccgta aagtcgatcc ccgagaaccg ggtggccccc
     1861 gtggtggagg tgaacgcgaa tctggtggcg gcgcccggtc cggggtcgaa cgcggaggag
     1921 ggcgcccgtg gtccaggttt ccaaagtttc aaattgggtt tccgacggtt tgccgtcggg
     1981 gtcgaccccc atacgccccg agccgaaagc gcgcaggggg tcccggaaaa ggcccgcggg
     2041 aacgttcatg ggcccccgca cggccgcgag gtattccttt tccacccggc ggttttcaaa
     2101 atcgagggag agggaacggt gggcgtcggc gtttttggcc aaaaccagga cgccgctcgt
     2161 gtcccggtcg aggcgatgaa ccacccaaac ggtccgtccg gcggcgcggg ccagctcctc
     2221 ccgcaggcac gggctcgttg cggggccccg gcccgggatg acctcctggc cggccggttt
     2281 gtccacggcc caataatcct cgttttccca cagaatccgt aggcccacgg gtcaacgggg
     2341 ggcggagaca aaggcgccgg gtttcggttc gcccagaagg gaaaggtcga cggggtcggg
     2401 aaatccggcc agggtcacgg cggggcggcg ctccagatgg aaaaacgccg ccacccgctg
     2461 ggcggtgtcg gggcccaaaa caatttcccc cgggccggcg accttttcga tgcgggcggc
     2521 gagattcacc gccgacccga tgacgctgta ttcgctccgg ccccgggcgc cgaggcaccc
     2581 cgccaggacc tcgccggtgt tcagggcgaa ccccacgtgt agaccgtcgg cctcgggggt
     2641 ttgccccatg gcgaaacgca ggagggcccg ggctttggtg gcggcccggg cggccgccgc
     2701 cgcgtggtcc tccagtttct gcggcgcgcc gaacacaccc atgacgccgt cccccagaaa
     2761 tttgttgacc accccgccct ggtcctgaat ggcccccacc agcacgtcga aaatttgatt
     2821 cagggattcc acggcctttt ccggggagac cattcgcgca tacggggtga acccccggac
     2881 atccgcgaag agaaccgtga cgtcccggcg ctcgccccgc ttccaaaaat cgtcggggtg
     2941 ttccatcacg aaacgggcga cttcgggcgt cgtaaattgg ccgaacaggt cccggacttt
     3001 ttgcatttgg gataattcga tgtaggcgcg gttcaattcc gcccgaaggg ttttttcccg
     3061 ttcgagctcc ttggacaagt gacgcttggc cgcgcaccgg tcgatggcca ttttaagggt
     3121 gtcgggcgaa aaaggcttga ccaggtagtc gtaggcgccc tcccgaacgg cgtggatcgc
     3181 ggaagtcaaa tcgggatagc cggtcataat gatcacgtcg gcgttggttt gggcccgcgc
     3241 gcgtttggtc aactcggtgc cgttggtggc cccgggcatg tcgagatcgg tcaaaatcag
     3301 ttcgt
//
LOCUS       DAOQLS010000167         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15690_length_3292_cov_3.902379, whole genome shotgun sequence.
ACCESSION   DAOQLS010000167 DAOQLS010000000
VERSION     DAOQLS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15690_length_3292_cov_3.902379"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="PK362_04195"
     CDS             <1..1331
                     /locus_tag="PK362_04195"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNC74069.1"
                     /translation="EVKAGEGPEGLITYMRTDSVNVAKEAQKEAVAFIEERYGKDHLP
                     PRPRIYKTKSKGAQEAHEAIRPTKPSRTPESVRSFLEPDQFRLYELIWQRFMASQMAD
                     AIFDTMTVDITASSYLFRATGHALKFPGFLAVYGEVADEDAKKDAEDEGKTLPPLKAG
                     DRVDLKELKPEQHFTEPPPRFNEASLVRVLEEHGIGRPSTYAPIIHTIVDRGYVRLED
                     RRFYPTELGRTVDVQLCAHFPEIVNVDFTAKIEEKLDQIAENTAVWTDVLREFYTPFE
                     RDLAKAETGMARVEIKPTPTDEKCPKCGSPMGLRENRFGRYLACSNYPTCKSTIPVDR
                     NMKKIVPEETGEVCPNCGKPMVMRQGRSFGRPSRYLACTGYPTCKTTFSVDKNGQKII
                     RPKPEMTDQKCQKCGKAMLKRVGARGPFLACSGFPRCRNIKPVAPAAPPA"
     gene            1331..2233
                     /locus_tag="PK362_04200"
     CDS             1331..2233
                     /locus_tag="PK362_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC74070.1"
                     /translation="MGIDEALRRYWLYLRGEKGLSPATLRAYRSDLADLEAHARSNWP
                     GLPVGRWDRARLRPFLAAWGERPYRRSTLLRKYNALGAFFRYLKTTGVLRDNPLDGIP
                     RPRRERRVPAFLSAPEMERLLALRWDGPSPARGRAILELLYSSGLRVSELTGLDTTHV
                     DIWAGTVRVFGKGSRERVVPVGERALGALRDYARERGLAWGDRAAIRGTPLFVNARGG
                     RLSSRSVGTLVAAWARRAAVERRVHPHVLRHSFATHLLDRGCDLRSVQEMLGHKNLST
                     TQIYTHVTTARLRQVYERAHPRAG"
     gene            2412..2837
                     /gene="mraZ"
                     /locus_tag="PK362_04205"
     CDS             2412..2837
                     /gene="mraZ"
                     /locus_tag="PK362_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNC74071.1"
                     /translation="MFLGGEYRHGLDEKRRLALPARLRQNIRRFVLTRGLEGCLSLYT
                     EAEWKKLLSKLDALPVSNKAHARAFKRLLISGAILSDVDGQGRLLVPESLGRYAGITR
                     NVTVVGMESRIELWSSERWEQYRRNAEKSAAKIAAEIDL"
     gene            2867..>3292
                     /gene="mraW"
                     /locus_tag="PK362_04210"
     CDS             2867..>3292
                     /gene="mraW"
                     /locus_tag="PK362_04210"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNC74072.1"
                     /translation="MNENVRHTPVLLAESLAGLNVRPGGIYLDLTVGLGGHAAAIAEA
                     VGPTGRVEGLDQDPAALALASERLKPWADRVRLTAGNFERFDENLGLQPASVDGILAD
                     LGVSSLQLDEAARGFGFSREGPLDMRMGATGETALEYLRR"
BASE COUNT          599 a         1022 c         1077 g          594 t
ORIGIN      
        1 tcgaggtgaa ggccggggaa ggccccgaag gtctcatcac gtacatgcgc accgactccg
       61 tgaacgtggc gaaggaggcc caaaaggagg cggtggcttt catcgaagaa cgttacggca
      121 aggaccatct cccgccccgg ccccggatct acaaaaccaa atccaaaggg gcgcaggagg
      181 cccacgaggc catccgcccc accaaacctt cgcgcacccc cgaatcggtc cggtcttttt
      241 tggagccgga tcaattccgc ttgtacgaac tgatttggca gcgctttatg gccagccaga
      301 tggccgacgc cattttcgac accatgaccg tggacatcac cgcgtcgtct tacctgttcc
      361 gggcgacggg ccacgcgttg aaatttcccg ggttcctggc cgtttacggc gaagtggcgg
      421 acgaagacgc caagaaagac gccgaggacg aggggaaaac ccttcccccg ctgaaggcgg
      481 gggaccgggt ggacttgaag gaattgaagc ccgaacagca ttttaccgag ccgccgccgc
      541 gcttcaacga agcgagcctc gtgcgggtcc tggaagagca cgggatcggg cgcccttcca
      601 cctacgcccc catcatccac accatcgtgg accggggcta cgttcgcttg gaggaccggc
      661 gattttatcc cacggaattg ggccggaccg tcgacgtcca actgtgcgcc catttcccgg
      721 aaatcgtgaa cgtggatttc accgcgaaga tcgaggagaa attggaccaa atcgccgaga
      781 acacggccgt gtggaccgac gttcttcggg aattttacac gcccttcgag cgggacttgg
      841 ccaaggcgga gaccgggatg gcccgggtgg aaatcaaacc gaccccgacc gatgaaaaat
      901 gcccgaaatg cggatcgccc atgggtcttc gcgaaaaccg cttcggccgg tacctggctt
      961 gttccaacta ccccacctgc aagagcacga ttcccgtgga tcggaacatg aaaaaaatcg
     1021 ttcccgagga aacgggcgaa gtctgcccca attgcggaaa gcccatggtc atgcgccagg
     1081 ggcggagttt cggtcgaccg tcccggtatc tggcgtgcac cggctaccca acctgcaaga
     1141 ccactttttc cgtggacaag aacggccaga aaatcatccg gccgaaaccg gaaatgaccg
     1201 accaaaagtg ccagaagtgc ggaaaggcga tgttgaagcg agtgggcgcc cgcggcccgt
     1261 tcctggcctg ctcgggtttt ccccgttgcc gcaacatcaa gcccgtcgct ccggcggccc
     1321 cgccggcctg atgggaatcg acgaagccct tcgtcggtac tggctttacc ttcggggaga
     1381 aaagggcctc tccccggcca cgctccgggc ctaccgttcc gatttggccg atttggaagc
     1441 gcacgcgcgc tcgaattggc cggggctccc ggtcggccga tgggaccggg cccgcctgcg
     1501 cccgtttctg gccgcctggg gcgagcgccc ctaccgccga tccaccctcc tccgcaaata
     1561 caacgccctc ggcgcttttt tccgctattt gaaaacgacc ggcgttcttc gggacaatcc
     1621 gctggacggg atcccccggc cccgacggga acggcgggtc cccgcgtttc tgagcgcgcc
     1681 ggaaatggag cgccttctgg ccctgcgttg ggacggcccg tcgccggccc gggggcgggc
     1741 gattctcgag cttttgtatt cctccggtct ccgggtgtcg gagctgacgg ggttggacac
     1801 gacgcacgtc gacatttggg ccggcaccgt ccgcgtgttc ggcaaagggt cgcgggaacg
     1861 cgtcgtcccg gtgggcgaac gggccctggg ggccctgcgg gactacgccc gggaacgcgg
     1921 gttggcctgg ggcgatcggg ccgcgattcg cggcacgccg ttgttcgtga acgcgcgggg
     1981 agggcggttg agttcccggt ccgtcggaac gctggtcgcc gcctgggccc gccgcgccgc
     2041 ggtggagcgc cgggtgcatc cgcacgtcct gcgccattcc ttcgcgacgc acctgctgga
     2101 ccgcggttgc gatctgcggt ccgtgcagga aatgctcggg cacaagaatt tatccaccac
     2161 gcaaatctac acccacgtca ccaccgcgcg cctccgtcaa gtatacgagc gggcccaccc
     2221 ccgcgccggc taaccgcctc cctcctcgaa tatccgtcga cccggagctt tttttgggct
     2281 ccggtggacg tccgtccgtt cgccgataaa aaaagcttga caaaactccg cgcggtattt
     2341 atagttgtcc caacggtggg gaattgtggt gagttgtggc tcaatgtgtg gaatagtgga
     2401 gagggcggca aatgtttctg ggcggcgaat accggcacgg attggacgaa aagcgccgcc
     2461 tggcccttcc ggcccggctt cgccaaaaca ttcgacggtt tgtgctgacg cggggactcg
     2521 agggttgctt gtccctttac acggaggccg aatggaaaaa actcctgtcg aaactcgacg
     2581 cgctgcccgt ttccaacaaa gcgcacgcgc gcgccttcaa gcggcttttg atttcgggag
     2641 cgatcttgtc ggacgtggat gggcaggggc gcctcttggt ccccgaatcg ttggggcgtt
     2701 acgccggcat cacccgcaac gtgacggtgg tcggcatgga atcgcggatc gaactctggt
     2761 cctcggagcg ttgggaacag tataggcgca acgcggagaa gagcgccgcc aaaatcgccg
     2821 ccgaaatcga tttgtaggcc cgcgtccggt ggacgggggg agttccttga acgaaaatgt
     2881 ccgccacacg cccgtgttgt tggcggagtc cttggcgggg ttgaacgtcc ggccgggcgg
     2941 gatttacctt gatttgacgg tcggtttggg cggccacgcg gcggccatcg ccgaagcggt
     3001 ggggccgacg gggcgggtgg agggtctcga tcaggatccg gcggcgctgg ccctggcgtc
     3061 cgagcgtctc aaaccttggg cggaccgggt tcgattgacg gccggcaatt ttgaacgatt
     3121 tgatgagaac ctgggtcttc agccagcgtc ggtggacggg atcttggcgg atttgggcgt
     3181 gtcctcgctc caattggacg aggcggcccg ggggttcggt ttctcgcggg aaggccccct
     3241 cgacatgcgc atgggggcca ccggcgaaac ggccctggag tatttgcggc gg
//
LOCUS       DAOQLS010000168         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15795_length_3279_cov_4.158499, whole genome shotgun sequence.
ACCESSION   DAOQLS010000168 DAOQLS010000000
VERSION     DAOQLS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15795_length_3279_cov_4.158499"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PK362_04215"
     CDS             <1..769
                     /locus_tag="PK362_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74073.1"
                     /translation="VLELWGAGRPFLRLEDPGSRLSRTDLLAWFQEKLLGPTGLSQGR
                     VFTSQSVAGHNKSLLAGFPNLNGYEALVPSAPWREIAESQGGVVQPTTGLEVERPDAP
                     PLSRLGLRYFVTAEATSLRWPVVWDAGALRVYENPDWGPLLTVAGGELLTYYRRRAEK
                     MTCRWRSDRPGSRAVWMETNDPGWRAWTRGGEVRIRDLNGVAQSVGWGETGATRVWWR
                     YRPAPFLWGLAGTLAAVAAGLWALGRRLAVGGGGGGL"
     gene            766..1485
                     /locus_tag="PK362_04220"
     CDS             766..1485
                     /locus_tag="PK362_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNC74074.1"
                     /translation="MRFLSVIVPTYNESANIGGLLRALPPLLAPWDYEIIVVDDNSPD
                     GTAGLVNQLRKDRLFEGRLRLLRRTRDRGLSPAVVEAFEWSTGRLLAVLDADRSHDET
                     LLPRLVESVGRDAWVAVGSRRVPGGGADQWPWYRQWTSSLATGVARALLGVALKDPMS
                     GFFALRRDVFEKSRSRLRPRGYKILLEILVRARVPADRVREFPYVFKDRRQGYSKLSP
                     GVAGAFVRQLLGLAADRWRNR"
     gene            1551..2024
                     /gene="greA"
                     /locus_tag="PK362_04225"
     CDS             1551..2024
                     /gene="greA"
                     /locus_tag="PK362_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNC74075.1"
                     /translation="MAGESFLTRQGYEKLRQDLTKLKERRGQLSVDIGEAREKGDLKE
                     NAEYHAAKEEQAKVQQRINELEEKLRSARIIEESAVQTGEIRIGATAHLKDVKSGESV
                     VYTLVDVSEADFSQGKISVQSPVAQALLGRKAGEKVTVRLAGGPLEYQVTKVTRG"
     gene            2075..3223
                     /locus_tag="PK362_04230"
     CDS             2075..3223
                     /locus_tag="PK362_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNC74076.1"
                     /translation="MTDKTETKDSHAPKGLEDVVAGPSSITFLDGLKGRMIYRGYDVI
                     QIADKTIFEEIVHLLWHGDLPNRAQLDALKLELAEARSLPPDVLTTMRQFPKKSHPMD
                     VLRAGVCLLAMTDSDTQDNSPAANRRKAARLVARMATLSAAWHRIRNGKTPLAPDPSL
                     SHSANYLYMVTGEKPDPVSVQAMDLYLSLLADHDLNASTFTARVIVSTLSDIHAALAG
                     AIGALKGPLHGGANEKAMEMFLEIGDPAKTEKWVEKAIAEKRKIMGFGHRVYKVEDPR
                     SGLLKKMAERMGEVKKEPRWYQISVKVADTVHRLKGLNTNVDFYSASVLYLLGIPIDL
                     FTTVFAVGRAAGWCAHVFEQLGDNRLIRPRSEYTGPVDRAFIPLEKRP"
BASE COUNT          577 a         1067 c         1051 g          584 t
ORIGIN      
        1 ggtcctggag ttgtggggcg cgggacggcc gtttcttcgg ctggaggacc cgggttcccg
       61 gctttcccga acggatttgt tggcgtggtt tcaagaaaaa ctcctgggcc ccacggggct
      121 ttcccagggc cgggtcttca cgtcccagtc cgtcgccggg cacaacaaaa gccttttggc
      181 cgggttcccc aatttgaacg ggtacgaagc gctcgtgccg tcggcccctt ggcgggaaat
      241 cgcggaaagc caaggggggg ttgtccagcc cacgaccggc ctcgaggtgg agcgacccga
      301 cgcgccgccg ttgagtcgac tgggtctccg ttatttcgtg acggcggagg cgacgtccct
      361 ccggtggccc gtggtctggg acgccggcgc ccttcgggtc tatgaaaacc cggattgggg
      421 gccgctcctg acggtcgcgg ggggcgagct cctcacgtat taccgccgcc gcgccgaaaa
      481 aatgacctgc cggtggcggt ccgaccgtcc gggcagccgc gctgtttgga tggaaaccaa
      541 tgacccgggg tggcgggcct ggacccgggg cggggaggtc cggattcgag acttgaacgg
      601 ggtggcccag tccgtcggat ggggggaaac gggagccacg cgggtgtggt ggcgctatcg
      661 gcccgcccct ttcctctggg ggctcgccgg gacgttggcg gcggttgccg ccggtctgtg
      721 ggccttgggc cgccggctgg cggtgggcgg cgggggaggg ggcctttgag atttctttcg
      781 gtcatcgtcc cgacctacaa cgagagcgcg aacatcggcg gcctcctccg ggcgttgccg
      841 cccctcctgg ccccctggga ttacgagatc atcgtggtcg acgacaattc gccggacggc
      901 acggccggcc tggtcaacca attgcgcaag gaccgtctct tcgagggtcg attgcgtttg
      961 ctgcgccgaa cccgggaccg ggggttgagt ccggccgtgg tcgaagcttt tgaatggtcc
     1021 acgggtcgcc tgttggccgt cctggacgcc gatcgatccc acgacgagac cctcctgccc
     1081 cgcctggtcg aatccgtcgg ccgggacgcc tgggtggccg tggggtcccg ccgggtgccg
     1141 gggggcgggg ccgaccagtg gccctggtac cgccagtgga cgagctccct cgccacgggg
     1201 gtcgcccggg ccctgctcgg cgtggcgctc aaggacccca tgagcggttt ttttgccctg
     1261 cgccgggacg tgtttgaaaa gagccgctcc cggttgcgcc cccgggggta caagattttg
     1321 ttggagatcc tcgttcgggc ccgggtgccg gcggaccggg tgcgggagtt tccctacgtg
     1381 tttaaagacc gccgccaggg gtactccaaa ctgagccccg gggtggccgg ggccttcgtt
     1441 cgacaactcc tgggattggc ggcggaccga tggcggaatc ggtgattttt tgtagaattc
     1501 ccccggagcc cacgccccgc gtgggtttaa ctatttaaag aggtgtcggc atggcgggcg
     1561 aatcgtttct cactcggcag ggatatgaaa agttgcgcca ggacctgacg aaactcaagg
     1621 agcgccgcgg ccaattgagc gtggacatcg gcgaagcccg ggagaagggc gatttgaagg
     1681 aaaacgccga ataccacgcg gccaaggaag aacaggccaa ggtccagcag cgcatcaacg
     1741 aattggagga aaagctccgt tccgcccgaa tcatcgaaga gtccgcggtt caaacggggg
     1801 aaatccggat cggggccacc gcccatctca aagacgtcaa aagcggcgaa tccgtggtct
     1861 acaccctggt ggacgtctcc gaagccgatt tttcccaggg aaaaatttcc gtccaatcgc
     1921 cggtggccca ggcccttctc ggtcgcaaag ccggggaaaa agtgacggtc cgcctggccg
     1981 ggggtccgtt ggaataccaa gtgacgaaag tcacccgcgg atgattttcg cttgagcgcc
     2041 ggccgatgcg tttgtataat cccagcgttc ttttatgacc gataaaaccg aaaccaaaga
     2101 ttcccacgcc cccaagggcc tcgaagacgt cgtcgcgggc ccctcctcca tcaccttcct
     2161 ggacggactg aagggccgca tgatttaccg cggctacgac gtcatccaga tcgccgacaa
     2221 aaccattttc gaggaaatcg tccacctgct ctggcacggc gacctgccga accgggccca
     2281 gttggacgcc ctgaagctcg agctcgccga agcgcgctcc ctgccgcccg atgtgctgac
     2341 gacgatgcgg cagttcccca aaaaatccca tcccatggac gtgctccggg ccggcgtgtg
     2401 cctcctggcc atgaccgatt ccgacaccca ggacaacagt cccgccgcca accgccgcaa
     2461 ggccgcgcgc ctggtggccc ggatggcgac gttgtccgcc gcctggcacc gcattcgaaa
     2521 cggcaaaacc cccttggcgc cggacccgtc cctgtcccat tcggccaact acctttacat
     2581 ggtgacgggc gagaagcccg accccgtcag cgtccaggcc atggatcttt atttgtcgct
     2641 cttggccgac cacgatttga acgcctccac cttcaccgcc cgggtcatcg tctcgaccct
     2701 gtcggacatc cacgcggccc tggccggcgc catcggcgcg ctcaagggcc ccctgcacgg
     2761 cggcgccaac gaaaaagcca tggaaatgtt tttggaaatc ggcgacccgg ccaaaaccga
     2821 aaaatgggtg gaaaaagcca tcgccgaaaa gcgaaagatc atggggttcg gccaccgggt
     2881 ctacaaggtg gaggaccccc gctccggact gctcaaaaaa atggccgagc gcatgggcga
     2941 ggtcaaaaaa gagccccgct ggtatcagat ttccgtcaag gtggcggaca ccgtgcaccg
     3001 cctcaaaggc ctcaacacca acgtcgattt ttattccgcc tccgtccttt acctcctggg
     3061 cattcccatc gacctgttca cgaccgtttt cgccgtcggc cgcgcggccg gttggtgcgc
     3121 ccacgtgttt gaacagctcg gcgacaaccg cctgatccgc ccccgttccg aatacaccgg
     3181 tccggtggac cgcgcgttca ttcccctcga aaaaagaccc tgatccccga cggggccggt
     3241 cccttgaacc ctttcccctt ttcccgacgc ggccccgcc
//
LOCUS       DAOQLS010000169         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15797_length_3279_cov_3.533809, whole genome shotgun sequence.
ACCESSION   DAOQLS010000169 DAOQLS010000000
VERSION     DAOQLS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15797_length_3279_cov_3.533809"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2353
                     /gene="rpoB"
                     /locus_tag="PK362_04235"
     CDS             <1..2353
                     /gene="rpoB"
                     /locus_tag="PK362_04235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNC74077.1"
                     /translation="QTNPLAEMTHKRRLSALGPGGLNRKRAGFEVRDVHHTHYGRLCP
                     IETPEGPNIGLITSLACYARINQYGLIETPYRKVEKGRVTNEIDYLTADKEDNYVIAQ
                     ANAPVDKNGKFTSELIACRHKGDFPQKAPDDIDYMDISPVQVVSTSAALIPFLEHDDA
                     NRALMGSNMQRQAVPLLFPERPLVGTGIEGRVARDSAAVEVARRGGVVVSAASDHVAV
                     WSDEGKPGVDLYNLRKYARSNQDTCLNQLPCVKQFEKVSKGDVLADGPSTQEGELALG
                     RNLLVAFMPWEGYNYEDAIILSERLVRDDVFTSIHISEFQVEARDTKMGAEEITKDIP
                     NVGAESLAHLDDTGIIRVGAEVAPGDILVGKVAPKGDQQTTPEERLLKVIFGKKAEDV
                     MDASLRVPPGVTGKILATKVFVRKEKMAKKDENKKMKEIDLALEEKLEILRADRKAQY
                     ADIDDRLAGGKISKAQAKEMKEMFEALADRRIDEAKRLAAREKENFKMGDELPVTVNR
                     IVKVYIASKRKIQVGDKLAGRHGNKGVVAKIVPPEDMPYMPDGTPIDVVLSPLGVPSR
                     MNVGQLLECMLGWAAHVMGVETVTPVFDGAKEEQIHDLMRQAKDKLRAKGYPEKYLPS
                     DDGRIQLFDGRTGDAFASKVTVGYMYILKLAHLVEDKIHARSTGPYSLITRQPLGGKA
                     QFGGQRFGEMEVWAIEGYGASHTLQEFLTVKSDDVQGRTKMYEAIIRGEVATEPGVPE
                     SFRVLVRELQSLGMNVELVKTDGAKDGGETAKKSAAKEAVAKE"
     gene            2416..>3279
                     /locus_tag="PK362_04240"
     CDS             2416..>3279
                     /locus_tag="PK362_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNC74078.1"
                     /translation="MNFTLFDAVRLTIASADQISSWSHGEVRKPETINYRTFKPERDG
                     LFCERIFGPVRDWECNCGKYKFIKYKGVVCDRCGVEVTESKVRRERMGHIELAVPVAH
                     VWFLRKSPSRMGTLLNLKLSDLEKVVYYARHIVLEDWKDADGKAKYKARQLLTDEEHH
                     KAYDDTKGKIKVGIGAGAIRQLLEEIDVKKEAVEVRANLKEATSEAEKTRLVKRLRIL
                     EGFVRSATRPENMVLTVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRIINRNNRLKHI
                     EGLRAPEVMVHN"
BASE COUNT          732 a         1074 c          972 g          501 t
ORIGIN      
        1 ccagacgaac cccctggccg aaatgaccca caaacgccgc ctctcggccc tcggccccgg
       61 cgggttgaac cggaaacgcg cgggcttcga agtccgggac gtgcaccaca cccattacgg
      121 gcgtctctgc cccatcgaaa cgccggaagg tccgaacatc gggttgatca cgtcgttggc
      181 gtgctacgcc cgcatcaacc agtacgggtt gatcgaaacc ccctaccgca aggtggaaaa
      241 aggtcgcgtg accaacgaaa tcgactattt gacggcggac aaggaagaca actacgtcat
      301 cgcccaggcc aacgcgccgg tggacaagaa cggcaagttc acctccgaat tgatcgcctg
      361 ccgccacaag ggcgacttcc cccagaaggc ccccgacgac atcgactaca tggacatctc
      421 gcccgtgcag gtcgtttcga cctcggcggc cctgattccc tttttggaac acgacgacgc
      481 gaaccgcgcc ctcatgggtt ccaacatgca acggcaggcc gttcccctgc tgttccccga
      541 gcgccccttg gtgggcacgg gcatcgaagg acgcgtggcc cgggattccg cggccgtgga
      601 agtggcgcgc cggggcggcg tggtggtgtc ggccgccagc gaccacgtgg ccgtttggag
      661 cgacgagggg aaacccggcg tcgacctcta caatttgcgc aaatacgcgc ggtccaacca
      721 ggacacctgc ctcaaccagc tgccctgcgt gaagcaattt gagaaggtgt ccaaaggcga
      781 cgtgctcgcc gacggtccct ccacccagga aggcgaattg gccctggggc ggaaccttct
      841 cgtggccttc atgccctggg aagggtacaa ctacgaagac gccatcatcc tctccgagcg
      901 cctggtgcgc gacgacgtct tcacctccat ccacatttcg gagttccagg tggaagcccg
      961 ggacaccaag atgggcgccg aggaaatcac caaggacatt cccaacgtcg gcgccgaatc
     1021 cctggcccac ttggacgata cgggcatcat ccgcgtcggc gccgaagtgg cccccggcga
     1081 catccttgtc ggcaaggtgg cccccaaggg cgaccagcag accacgccgg aagaacgttt
     1141 gctgaaggtc atcttcggca aaaaagccga agacgtgatg gacgcctccc ttcgggtgcc
     1201 ccccggcgtc acgggcaaaa ttttggcgac caaggttttc gtccgcaagg aaaagatggc
     1261 caaaaaagac gaaaacaaga aaatgaagga aatcgatttg gcgctcgagg agaagctcga
     1321 aattctccgc gccgaccgca aggcccagta cgccgacatc gacgaccgcc tggccggcgg
     1381 caagatttcc aaggcccagg ccaaggaaat gaaggaaatg ttcgaagccc tggccgaccg
     1441 gcggatcgac gaagccaagc gcctggccgc ccgggaaaaa gaaaatttca aaatgggcga
     1501 cgaactgccc gtcacggtga accgcatcgt gaaggtctac atcgcctcca agcgcaaaat
     1561 ccaggtcggc gacaagctgg ccggccgcca cggcaacaag ggcgtcgtcg ccaagatcgt
     1621 tccgcccgaa gacatgccct acatgccgga cgggacgccc atcgacgtcg tgctctcgcc
     1681 cctcggcgtg ccctcccgga tgaacgtcgg ccaattgctc gaatgcatgc tcggttgggc
     1741 cgcccacgtc atgggggtgg aaaccgtgac cccggtgttc gacggcgcca aggaagagca
     1801 gatccacgat ttgatgcgcc aggccaagga caagctccgc gcgaagggct atccggaaaa
     1861 gtacctgccc tccgacgacg gccgcatcca gctgttcgac ggacgcaccg gcgacgcctt
     1921 cgccagcaaa gtcaccgtgg gctacatgta catcctgaag ctcgcccacc tggtggaaga
     1981 caagattcac gcccgctcga ccggcccgta ctcgctcatt acgcgccagc cgctcggcgg
     2041 caaggcgcaa ttcggcggcc agcgcttcgg cgaaatggaa gtctgggcca tcgaaggcta
     2101 cggcgcctcc cacaccctgc aggaattttt gacggtcaag tccgacgatg ttcagggccg
     2161 gaccaagatg tacgaagcca tcatccgcgg cgaagtcgcc acggagccgg gcgtgccgga
     2221 aagcttccgc gtcctcgtgc gcgagttgca gtccctgggc atgaacgtcg agttggtgaa
     2281 aaccgatggg gccaaagacg gcggcgaaac cgccaagaag tcggcggcca aagaagccgt
     2341 ggcgaaggag taaccgtgac gacccttttt gccgaacgta aaaaagaatc caccaagaag
     2401 aaggcccccg ccgggttgaa tttcaccctg ttcgacgcgg tgcgtctgac catcgcctcc
     2461 gcggaccaga tttcctcctg gagccacggc gaagtgcgga agcccgaaac catcaattac
     2521 cgcaccttca agcccgagcg cgacggtctc ttctgcgagc gcattttcgg ccccgtgcgg
     2581 gactgggagt gcaactgcgg caagtacaag ttcatcaagt acaaaggcgt cgtgtgcgat
     2641 cgttgcggcg tcgaagtcac cgaatccaaa gtccgccgcg agcggatggg ccacatcgag
     2701 ttggccgtcc ccgtggccca cgtgtggttc ctgcgcaagt cgccctcccg catgggcacg
     2761 ctgctcaatt tgaagctgtc cgacctggaa aaggtcgtct actacgcccg gcacatcgtg
     2821 ctcgaggact ggaaagacgc cgacggcaaa gccaagtaca aggcgcgcca gctcctgacc
     2881 gacgaggaac accacaaagc ctacgacgac accaaaggca agatcaaggt gggcatcggc
     2941 gccggcgcca tccgccaatt gctggaagaa atcgacgtca agaaagaagc cgtcgaagtc
     3001 cgcgcgaatt tgaaggaagc cacctcggaa gccgaaaaga cccggctggt caagcgtctc
     3061 cgcatcctgg agggcttcgt ccgctcggcc acccggcccg agaacatggt gctgacggtc
     3121 ctgccggtca ttcccccgga cctccgcccc ttggtgcccc tggaaggcgg ccgtttcgcc
     3181 acttccgacc tcaacgattt gtaccgccgc atcatcaacc ggaacaaccg gttgaagcac
     3241 atcgagggcc tccgcgcccc cgaagtgatg gtgcacaac
//
LOCUS       DAOQLS010000170         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_15934_length_3264_cov_3.415083, whole genome shotgun sequence.
ACCESSION   DAOQLS010000170 DAOQLS010000000
VERSION     DAOQLS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15934_length_3264_cov_3.415083"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PK362_04245"
     CDS             <1..505
                     /locus_tag="PK362_04245"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNC74079.1"
                     /translation="EDAQCLFDVVDGKRREAELRPNGLLAVSLPYEILEEKNFKPLVE
                     TTWWELLTPRGLRTLSPSHLLYRGRHGGPPADRDRMYHQGTVWPWLLGPFVTAYVKAF
                     GSTEESKKQVVRFLQPLVHHMSEAGLGQVSEFFDGDAPHAPQGCPAQAWSVGEILRVL
                     WEEGITL"
     gene            570..1634
                     /locus_tag="PK362_04250"
     CDS             570..1634
                     /locus_tag="PK362_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNC74080.1"
                     /translation="MKKILLALVLCGGARGARAEGVEILFGPGGADRHALASAPTVST
                     VPTGEFVVASPVVDLRRDPAPVAEGAGARRPYAVDPLQETQVLYGETVYSFEEKDGWV
                     RVEAPEQPEYTHGDRWRGYPGWVPRSSLEPRPSQYEPNGVVTARYGLVRPNRRWFSKG
                     LALPMGSRLYLAYKKGRWYRVAGVRGESGWMRTKEARTTVDTPRRAADIRGVLLRSAE
                     KFLGEPYYWGGRAGHRAEENAPSGVDCSGLVNVAYRVAGLEIPRDAHEQFMASRAIAR
                     SSELEPGDLVFLAKTSKPDRMVHVMIFAGGEQMIEAVHEENVVRRVTFKKKLGEAKST
                     LRPGQTAGGYVVTFGRLIPR"
     gene            1631..2710
                     /locus_tag="PK362_04255"
     CDS             1631..2710
                     /locus_tag="PK362_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNC74081.1"
                     /translation="MTPTAIAAVDVRPVRWPLRRPFVTALGRKTHSENVLVAVRLTGG
                     LVGRGEASSSLAMPFQTGHRMASALRRLAPWSRGRDVRDIRSLSLALWRREGAWPTAA
                     AAFEAALWDALAQSEGVPLYALWGKKQTEIETVLSVSAVDPAEVGRRAARAGRAGYRK
                     IKLKLNGAEGAAINIERVRRAFQGAPRARFLLDPNQSLTPVLLEEILAGARRDGVPLA
                     AVEEPFPKHRWDLLEEARPPCPLILDESIQSARDARIAYRRDGVSGANIKLAKSGVLR
                     SFDIVDETRAARGRRARLMIGCMAESRLGLAAAVHFALGTGVFDEADLDSDLILRPTP
                     FRGGYVRRGPWIRLPRRPEPGLGVR"
     gene            2707..>3264
                     /locus_tag="PK362_04260"
     CDS             2707..>3264
                     /locus_tag="PK362_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HNC74082.1"
                     /translation="MSSPWMLAAVLAAVVLGLVGLRDRRPRLFQYLPVPFWCYVLPMI
                     GGTFRLFPEASPVYTLLGRYALPFCLSLLLINVDLRALVRLGRPAVLAMGLGVAGIGM
                     GAVLGLFLFHGNLPPGAWKAVGALSASWTGGSANMLAVKEALSAPEAVFAPVVVIDSF
                     FAYAWMAGLISLAGFQESFDRWVGAG"
BASE COUNT          477 a         1064 c         1121 g          602 t
ORIGIN      
        1 cgaggacgcc caatgcctct ttgacgtcgt ggacggcaaa cgccgggagg cggaactccg
       61 ccccaacggc cttttggccg tgagcctccc ctacgaaatc ctggaggaga agaatttcaa
      121 gccgttggtg gaaacgacct ggtgggaact tctgacgccc cgggggctgc gtaccctgtc
      181 gccgtcgcac ctcctctacc gcgggcgcca cggcgggccc ccggcggacc gcgaccgcat
      241 gtaccaccag ggcacggtgt ggccctggct gttgggcccc ttcgtcacgg cgtacgtcaa
      301 ggcgttcggt tccacggaag agtccaaaaa gcaagtcgtg cggtttctcc agcccctggt
      361 ccaccatatg tcggaggcgg gccttggcca ggtatcggaa ttttttgacg gcgacgctcc
      421 ccacgctccc caggggtgcc cggcccaggc ctggagcgtg ggcgaaatcc ttcgcgtgtt
      481 gtgggaagag ggcatcaccc tttgagcgga ccgctcggcg ggaaaaaaat cgcgggatcc
      541 ggcccctggg ggccgacgaa ggcggaacga tgaaaaaaat cctcttggcg ttggttttgt
      601 gcggcggggc ccggggcgcc cgcgccgagg gggtggagat tttgttcggt ccggggggcg
      661 ccgaccggca tgccctggcc tccgcgccga cggtttcgac ggtcccgacg ggggagttcg
      721 tggtggcgag ccccgtggtc gaccttcgcc gggatccggc gcccgtggcc gaaggcgcgg
      781 gggcccgacg cccgtacgcg gtggatccgc ttcaggaaac gcaggttttg tacggcgaga
      841 ccgtctattc ctttgaggaa aaggacgggt gggttcgggt ggaggccccc gagcagccgg
      901 agtacaccca cggcgaccga tggcggggtt accccggctg ggttccccgg tcgtccctgg
      961 aaccccggcc gtcccaatac gagcccaacg gggtcgtgac ggcccgctac ggccttgtcc
     1021 ggcccaatcg gcgctggttc tccaagggac tggccttgcc catgggatcg cggctttacc
     1081 tggcttacaa aaagggccgg tggtatcggg tagccggcgt ccggggcgaa agcgggtgga
     1141 tgcggaccaa agaggcgcga acgacggtgg ataccccgcg gcgggccgcg gacattcgcg
     1201 gggtccttct tcgttcggcg gaaaaatttc tcggcgagcc gtactactgg ggcgggcggg
     1261 ccggccatcg cgcggaggaa aacgcgccca gcggcgtgga ctgctccggc ctcgtcaacg
     1321 tcgcttaccg ggtcgccggg ttggaaatcc cccgggacgc ccacgaacaa ttcatggcga
     1381 gccgggccat cgcccggtcg tcggaactgg aaccgggcga tttggtcttc ctggccaaaa
     1441 cgtccaagcc ggaccgcatg gtgcacgtga tgatttttgc cggcggggag caaatgatcg
     1501 aggcggtgca cgaagagaac gtcgttcgcc gggtcacctt taaaaagaaa ttgggcgagg
     1561 cgaaatcgac gcttcgcccc ggacaaaccg ccggggggta tgtcgtgacc ttcggacgac
     1621 tcattccccg ttgaccccca ccgccatcgc ggccgtcgat gttcgcccgg tccgctggcc
     1681 cctgcgccgt cccttcgtca cggccctggg acgcaagacc cattccgaaa acgtcctcgt
     1741 cgccgttcga ttgacgggcg gccttgtcgg tcgtggggag gcctcctcct ccctggccat
     1801 gccttttcaa accggacacc ggatggcctc ggcgctgcgg cggctcgccc cctggagccg
     1861 gggccgggac gtccgggaca tccgttcgtt gagcctcgcc ttatggcggc gggaaggcgc
     1921 ctggcccacg gccgccgcgg cctttgaagc ggccctttgg gacgccctgg cccaatcgga
     1981 aggcgttccg ctttacgccc tgtggggaaa aaaacagacg gagatcgaaa ccgtcctcag
     2041 cgtttccgcg gtggaccccg cggaggtggg ccgtcgggcg gcccgcgccg gccgggcggg
     2101 ttaccgaaaa ataaaactta agctgaacgg cgccgagggg gccgcgatca acatcgaacg
     2161 cgtgcgccgc gcgtttcaag gggcgccccg ggcccgattc ctattggacc cgaaccaaag
     2221 cctgaccccc gtcctgttgg aggagatcct ggccggcgcc cggcgggacg gcgttcccct
     2281 ggcggcggtg gaggaaccct ttcccaaaca ccggtgggac cttctcgaag aagcccggcc
     2341 cccctgcccc ctgattcttg acgaatccat tcaatccgcc cgggacgccc ggatcgccta
     2401 tcggcgggac ggcgtgagcg gcgcgaacat caagctggcc aaaagcggcg ttctccggag
     2461 tttcgacatc gtcgacgaaa cccgcgccgc ccgcggtcgc cgggcgcgcc tcatgatcgg
     2521 ctgcatggcc gagtcccgcc tcgggttggc ggccgcggtc catttcgccc tgggcacggg
     2581 cgtttttgac gaggccgatt tggattcgga tttgatcctg cgtccgacgc cctttcgggg
     2641 cggttacgta cgccggggcc cgtggattcg tttgccccgc cgccccgaac ccggtttggg
     2701 ggttcggtga gttccccctg gatgttggcc gcggtcctgg ccgctgtcgt cctggggttg
     2761 gtcggccttc gggaccgccg tccccggttg tttcaatacc ttcccgttcc tttttggtgc
     2821 tacgtgcttc cgatgatcgg gggaacgttc cgcctttttc cggaggcgtc gcccgtttac
     2881 acccttctgg gccgatacgc cctgcctttt tgtttgtcgt tgctcttgat caacgtcgat
     2941 ttgcgggccc tggtccgttt gggccgtccg gcggtcctgg ccatgggcct cggggtggcg
     3001 ggcattggga tgggggccgt gctgggcctt ttcctctttc acgggaacct gccgccggga
     3061 gcctggaaag ccgtcggcgc tctctcggcc agttggacgg ggggaagcgc caacatgctg
     3121 gccgtcaaag aagccctgtc ggcgccggag gccgttttcg cccccgtcgt cgtgatcgat
     3181 tcctttttcg cctacgcctg gatggcgggc ctcatttccc tggccggatt tcaggaatcc
     3241 ttcgaccggt gggtcggcgc cggg
//
LOCUS       DAOQLS010000171         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16094_length_3244_cov_3.699592, whole genome shotgun sequence.
ACCESSION   DAOQLS010000171 DAOQLS010000000
VERSION     DAOQLS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16094_length_3244_cov_3.699592"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..466)
                     /locus_tag="PK362_04265"
     CDS             complement(86..466)
                     /locus_tag="PK362_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC74083.1"
                     /translation="MTIPPDKEYFSIGEVSRLSGVKPHVLRYWERAFGLVRPARRGTG
                     HRRFGRRDVETIGRIRELVEDRRLTLEGAKKELRAQNRRGAEQIPLAFKETSAAVEVL
                     KELKRDMVELLSELKRPLSTDSKS"
     gene            591..1064
                     /locus_tag="PK362_04270"
     CDS             591..1064
                     /locus_tag="PK362_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC74084.1"
                     /translation="MTHIHRRSGFTLIELMLVVAIIGLLAAIAVPKFANLVIKAKEAS
                     TRGKLGTVRSALSIYYADNEGRYPFDDGSGQALAHALLPKYLSEIPRIEVPTVPAHIL
                     GNQDTGVTPLFNMPEFGGPTCYAFRTTATMPFQLTGEIKVACTHTDTKGTTWSLY"
     gene            complement(1066..1764)
                     /locus_tag="PK362_04275"
     CDS             complement(1066..1764)
                     /locus_tag="PK362_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNC74085.1"
                     /translation="MRRALRRAARPDKIAILSSFFKSGPGDYGAGDRFLGVPVPTQRR
                     VARDHRALPLDAVVDLLRGPIHEERLTALLILLEKFKIGSPAERSALHRFYVRHLHRV
                     NNWDLVDCSAATLVGEALEGKSHALLDRLARSGNLWDRRVAMIATHYFTKKGDPKPAL
                     RLARRLLSDPHDLMHKAVGWMLREVGKRCGEAFLTTFLDRHGAAMPRTALRYAIERFP
                     FHRRRFYLRGGRRG"
     gene            complement(1796..2053)
                     /locus_tag="PK362_04280"
     CDS             complement(1796..2053)
                     /locus_tag="PK362_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNC74086.1"
                     /translation="MNKADLAAVVSNVVGTQMEAKAAVTQLFESMRKALRDGDKVVVQ
                     GFGSFHVVMRKSKPARNPRTGEAVMIPPRRRVKFKMAKDLL"
     gene            complement(2068..3099)
                     /locus_tag="PK362_04285"
     CDS             complement(2068..3099)
                     /locus_tag="PK362_04285"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNC74087.1"
                     /translation="MTDRVAVLGGGSWGATLADHLARQGREVTVWEFNRERAESLQKT
                     RRLSFMPQLELHPKAVVTNDLGAALEGRPAIVSAVPSEFVRQTFRAVRSTGRLAPGAW
                     AVSVTKGIENNSLKRMTEVIVDEEPALKDRVSVLAGPSHAEEVAKSLPTAVVAAGPEP
                     FRERIVALFNTDRLRVYTSPDFVGVELAGALKNVYAVACGVSDGLGMGDNTKAALMTR
                     GLNEIARIGAACGAHVITFFGLAGLGDLIVTCTSRHSRNRAFGEKIGQGKTVPQALAE
                     MTMVAEGLPTAKSAKQLADRFDLDVPIIHALYGCLYEGKTAREAVKELLARPPVEEMQ
                     HLEQFVQNK"
BASE COUNT          621 a         1079 c          936 g          608 t
ORIGIN      
        1 ttgaacccac gacctctcgc accccaagcg agtgcgctac cactacgcta cgccccgaaa
       61 aatggttttg cggacggccc gacggtcagc ttttcgaatc cgtcgaaagc ggccgcttca
      121 attcgctcag caactcgacc atgtcgcgct tcagttcctt caacacttcg acggcggccg
      181 aggtttcctt aaaagccagg ggaatctgtt ccgccccccg tcgattttgc gcccgaagct
      241 cttttttggc cccttccagc gtcaggcggc gatcttccac caattcccga atgcgcccga
      301 tggtttcgac gtcccgccgt ccgaatcgcc gatggccggt gccccgccgc gcgggacgaa
      361 ccaagccgaa ggcgcgctcc caataacgga ggacatgagg tttgacgccg gagagccgcg
      421 acacttcgcc gatggaaaaa tattccttgt ccggcggaat ggtcaacaac attgcgttaa
      481 ttaaaccaaa atcgggaagt ctttacaacg cgtttttggc ggggagcgtc cgtcgacgaa
      541 agacttggcg aaacgccttt ggggggttac ccttgaagcg agaggttcca gtgacccata
      601 tccatcgacg atccggtttc acattgattg aattaatgct ggtggtcgcg atcatcgggc
      661 tgctggcggc catcgccgtt cccaaattcg ccaatctcgt gatcaaagcc aaggaagcct
      721 ccacccgggg caagttgggg acggtccgtt cggccctcag catctactac gccgacaacg
      781 aaggccggta tcccttcgac gacgggtccg gccaggccct ggcccacgct ctgcttccaa
      841 aatatctttc ggaaattccc cggattgaag tccccacggt tcccgcgcac atactgggga
      901 atcaagacac cggcgtgacg cccttattca atatgcccga attcgggggc ccgacctgtt
      961 acgccttccg cacgacggcg acgatgcctt tccagctcac cggcgaaatc aaagtggcct
     1021 gcacccacac ggacaccaag gggaccacgt ggtccctcta ttgaatcaac cgcgccgccc
     1081 gccgcgcaaa taaaaacgac gtcggtggaa cggaaagcgt tcaatcgcgt aacgcagggc
     1141 cgttcggggc atcgccgcac cgtgccgatc aaggaatgtc gtgaggaatg cctccccgca
     1201 tcgctttccc acctcccgga gcatccaccc cacggcctta tgcatcagat cgtgcgggtc
     1261 ggacaggagc cggcgcgcga ggcggagggc gggtttggga tcgcccttct tcgtaaagta
     1321 gtgcgtggcg atcatggcca cgcgccggtc ccacaaattt ccggaacggg ccaatcggtc
     1381 cagcaacgcg tgggactttc cctccaacgc ttcgccgacc aaagtcgccg cggaacaatc
     1441 caccagatcc caattgttca cgcggtggag atgccgcacg taaaaccggt gcagcgcgga
     1501 acgctccgcg ggggaaccga ttttgaattt ttcgaggaga atcaggagcg cggtcaaccg
     1561 ttcctcgtgg atcgggccgc gcagcaggtc gaccacggcg tccagaggca gcgcgcgatg
     1621 gtcccgggcg acgcgccgct gagtcggaac gggcaccccc aaaaaccgat cgcccgcgcc
     1681 gtagtccccc gggccggatt taaagaaact cgaaaggatc gcgattttgt ccggccgcgc
     1741 ggcccgccgg agggcccggc gcacggcgga agcccccgaa ccggggcggg tcatctcaga
     1801 ggaggtcctt cgccatcttg aatttcaccc ggcgtcgggg cgggatcatg accgcttccc
     1861 ccgtgcgggg gttgcgggcc ggtttgcttt ttctcatcac gacgtgaaaa ctgccgaacc
     1921 cttggacgac cactttatca ccgtcccgaa gggcctttcg catcgattca aacaactggg
     1981 tcaccgcggc ttttgcctcc atctgcgtgc ccaccacgtt ggacaccacc gccgccaaat
     2041 ccgctttatt cataccgccg tttcctccta tttgttctgg acaaattgtt cgaggtgctg
     2101 catttcttcc acgggagggc gcgccaaaag ttctttgacg gcctcccggg ccgttttgcc
     2161 ttcgtacaaa cacccgtaca aagcgtgaat gatcgggacg tccaggtcga aacgatcggc
     2221 caattgcttg gcgctcttgg ccgtgggcag gccctccgcc accatggtca tttccgccag
     2281 ggcctggggg accgttttcc cctggccgat cttttcgcca aaagcccggt tccgggaatg
     2341 gcgcgaggtg cacgtcacga tcagatcccc cagccccgcc aatccgaaga aggtgatgac
     2401 gtgggccccg caggccgccc cgattcgcgc gatttcgttc aacccgcggg tcatgagcgc
     2461 cgcttttgtg ttgtcgccca tgcccaggcc gtcggagacg ccgcaggcga cggcgtacac
     2521 attcttcaag gcgccggcca actccacgcc gacgaagtcc gggctcgtgt agacccgcag
     2581 gcgatccgtg ttgaaaaggg cgacgattcg ttcccgaaac ggttcggggc cggccgcaac
     2641 caccgccgtc ggcagggatt tggccacctc ctccgcgtgg gacggacccg ccaggacgga
     2701 aacgcggtct ttcaaggccg gttcctcgtc gacgatcact tccgtcatcc gtttcaacga
     2761 attgttttcg atgcccttcg tgacgctaac ggcccaggcc ccgggcgcga gccgcccggt
     2821 ggaccgcacg gcccggaacg tttgccggac gaattccgac gggacggcgc tcacgatcgc
     2881 gggccgccct tccagggccg cgcccagatc gttggtcacg accgccttgg gatgaagttc
     2941 caattgaggc atgaaggaaa ggcgccgggt tttctgaagg gattcggccc tttcccgatt
     3001 gaattcccac accgtcactt cgcgcccttg ccgggccaga tgatccgcca acgtcgcccc
     3061 ccaactgcca ccgcccaata ccgcgacgcg atccgtcatg atgccgccct gttgtccgtg
     3121 ttcccgaagc gaaccggagg ttcttccccg cgcatcagcc gccggatgtt ggggaggtgt
     3181 ttaaaaataa cgaaggaacc gaggagcagg gccaagttcc gctccgccgc gggcgccccg
     3241 cgcc
//
LOCUS       DAOQLS010000172         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16193_length_3233_cov_4.058213, whole genome shotgun sequence.
ACCESSION   DAOQLS010000172 DAOQLS010000000
VERSION     DAOQLS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16193_length_3233_cov_4.058213"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PK362_04290"
     CDS             <1..950
                     /locus_tag="PK362_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74088.1"
                     /translation="SFYAGKTLDTSVYAADRLYLCGNSTAGDCAGNVAPGPGMTLTNG
                     PYIAANHLHLGSNNTLAAGTPAYYPWGPGVRKDGMGSYLGPTYAFTPEVSLVAPNLTY
                     YMTRNNFLVSPASPTFNVLEFQTGGANGRVYFPNAGYTFDVPTNFSPQGVPSAVIVST
                     GMDLTITGDATVRGRFTIVCLGCNVTVLNDLTYHQTPSPLPVAGCGLCATPNDALAVL
                     TDGQIRFESSATDTTPAEQTVAGLFYSSTGQLMKQSPDATWANLKVYGTLVGFMGPDP
                     APGNATQLSVRQDPNLRYYLPPMLPERPVMVTRANRAWW"
     gene            complement(1088..2434)
                     /locus_tag="PK362_04295"
     CDS             complement(1088..2434)
                     /locus_tag="PK362_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74089.1"
                     /translation="MVVLILALLAIFLPSMVQMSKDDSRWSTRKMHVDEIITIAEAGL
                     DRGSWTLGDSETNWQKAKSGVVVTEYNYDRVWTDLDPAYQYKIKFSSGPGPEEVTVRC
                     KVQSLRDRRMIRTLKAVYTRNFVEALVMKNDLDASSDVYPRVHWGPIKSYAGLGLTPS
                     FLTTDVPHYPRKYARNGIFARDSVRDDHNSDAQEYWAFDKNIGDPPTIDYQYYRYRAA
                     GSAFPRLTAGLGTLVMRDGVTAAVSASGANTGLFLCADNWDGSSRGVRFRTDVGQNYV
                     FSNSTSVFFVENSTNASCRTNIEGKVFLDLEAFIVGGSNNDLWMHPHWASYNATIPVN
                     ADLEYRQTDASTTWVGSGMGAVWSAGRYPYNIPDKVGFHGFLHVQDGLCTIEDNNDDI
                     RLLGVVYVKTLKTDFDPPGQGLDIYFDENVASQMKVIYTPVRRRYYNEDKSDMVWP"
     gene            complement(2487..3014)
                     /locus_tag="PK362_04300"
     CDS             complement(2487..3014)
                     /locus_tag="PK362_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC74090.1"
                     /translation="MTRRRSRFRRSGGFTFIEVLVTLAILAVMVAVLPGLLDKVVRFY
                     YLTMDRLTLQRDARNATEIVVTGLRKAVSTTICVDQVDAPVPQPPYSRIRFETVRGST
                     VTVWQEGRNLYVTDSVSSPEPRVVTPYLRFLAFSYVGSMDDSVMSVNLSLEKESFRRR
                     RIFYVTNDRVRILNE"
     gene            complement(3011..>3233)
                     /locus_tag="PK362_04305"
     CDS             complement(3011..>3233)
                     /locus_tag="PK362_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74091.1"
                     /translation="TSQFRAGGLEYYMTASNAAGRYTFDVKMGTYNVYGFYPYFNTAG
                     DVTWVKRSISAFINSGNNATDFPVLDLTP"
BASE COUNT          646 a         1016 c          955 g          616 t
ORIGIN      
        1 actcctttta cgccggcaaa accctggaca cgagcgttta cgcggcggac cgcctatacc
       61 tttgcggcaa ttccaccgcc ggggactgcg cggggaacgt cgcccccggc cccggcatga
      121 cgttgaccaa cggcccctac atcgcggcga atcaccttca tttggggtcc aacaacacct
      181 tggccgccgg aaccccggcc tattacccct gggggccggg ggtccgcaaa gacggcatgg
      241 gctcctatct ggggcccacc tacgccttta cccccgaggt gagcctcgtg gcgcccaatt
      301 tgacctatta catgacccgg aacaatttcc tggtgtctcc ggcctccccg acgtttaatg
      361 tcttggaatt tcaaacgggc ggagcgaacg ggcgtgttta tttccccaac gcggggtaca
      421 cgttcgatgt tccaacgaac ttcagccccc agggcgtccc gagcgccgtc attgtctcca
      481 cgggaatgga tctgacgatt acgggggacg ccacggtccg gggacgattc accatcgttt
      541 gcctgggatg caacgtgacg gtcctgaacg acttgacgta ccaccaaacc ccgtcgccgc
      601 tgcccgtggc cggatgcggc ctttgcgcca cgcccaacga cgccctggcc gtgttgaccg
      661 acggtcaaat tcgattcgaa agcagcgcga ccgacaccac cccggcggaa caaacggtgg
      721 cgggcctctt ctattccagc accggtcaat tgatgaaaca gagtcccgac gccacctggg
      781 ccaatctcaa agtctacgga accctcgtgg gatttatggg gccggacccc gcgccgggca
      841 acgccaccca attgtcggtt cgccaggatc ccaatctccg gtattacctc ccccccatgc
      901 tgccggaacg gccggtcatg gtcacccggg ccaatcgcgc ctggtggtga ccgtcccgcc
      961 ttttcccgcg cgaccccatc cgggccgcgc catccactcc ccccgtcccc ttcaataaaa
     1021 aaaccgcccc cgggaggggg gcggtcgacg gcgcgcgccg aacatctcag cgcccgggac
     1081 cgacgattta aggccagacc atgtcggatt tgtcctcgtt gtagtatcga cgacggacgg
     1141 gcgtgtagat caccttcatt tgggaggcca cgttctcgtc gaaataaatg tccagcccct
     1201 ggcccggggg atcgaagtcg gttttgagcg ttttcacgta aaccacgccg agcagtcgaa
     1261 tgtcgtcgtt gttgtcctcg atcgtgcaca ggccgtcctg cacgtgcagg aacccgtgga
     1321 accccacttt gtccgggata ttgtaggggt accgccccgc ggaccacacc gcccccatgc
     1381 ccgaacccac ccaggtggtg gaggcgtccg tttgccggta ctcgaggtcg gcgttcaccg
     1441 gaatggtggc gttataactt gcccagtggg ggtgcatcca gaggtcgttg ttcgatccgc
     1501 cgacgatgaa ggcctccaga tccagaaaca ccttcccttc gatgttcgtc cggcaactgg
     1561 cgttggtgga gttctcgaca aaaaagaccg acgtggaatt ggagaagacg tagttctggc
     1621 cgacgtccgt acgaaatcgg accccgcggc tggacccgtc ccaattgtcc gcgcacaaaa
     1681 acaaccccgt gttggcgccg ctggccgaca ccgcggccgt aaccccgtcc cgcatgacga
     1741 gcgttcccag gccggcggtc aaccggggga acgccgagcc ggcggcccga taacgataat
     1801 attgatagtc gatggtcggc gggtccccga tgtttttgtc gaacgcccag tattcctgcg
     1861 cgtcgctgtt gtggtcgtcc cgaacgctgt cccgggcgaa gataccgttc cgcgcgtact
     1921 tgcggggata atggggcacg tccgtggtga gaaaggaggg ggtcaacccg agaccggcgt
     1981 aactcttgat cggcccccag tggacgcggg ggtaaacgtc ggacgacgcg tccaggtcgt
     2041 ttttcatgac cagggcttcg acgaaatttc gggtatacac ggcctttaac gtgcggatca
     2101 tccggcggtc ccgcagggac tggactttgc accgcaccgt cacttcctcc ggtcccgggc
     2161 cggaggagaa tttaattttg tattgatagg cgggatccag gtccgtccac acgcggtcgt
     2221 aattgtattc ggtgacgacc accccgctct tggctttttg ccaattcgtt tccgaatccc
     2281 cgagcgtcca ggagccccgg tccaaaccgg cttcggcgat cgtaatgatt tcatcgacgt
     2341 gcattttccg cgtcgaccag cgcgaatcgt ccttgctcat ctgaaccatg ctcggtaaaa
     2401 agatggcgag cagggccaat attaaaacaa ccatcgggag gaagaacccc ccgtgggatt
     2461 tccgtcgtcg cccgggcgtt ccgcgcttat tcattgagga ttcggacgcg gtcgttggtc
     2521 acgtaaaaga tccgcctccg tcggaaggat tccttctcca aggagaggtt cacggacatc
     2581 acgctgtcgt ccatggaccc cacgtaagag aaagccagga atcgcaaata gggggtcacc
     2641 acccggggct cgggcgagga cacagagtcc gtgacgtaca aattgcggcc ctcctgccat
     2701 accgtgacgg tggacccccg gacggtttca aaccgaatcc gcgaataggg cggctgggga
     2761 acgggggcgt ccacctggtc cacgcagatc gtggtggaaa cggccttccg caaaccggtg
     2821 accacgattt cggtcgcgtt gcgggcgtcc ctttgcaacg tcaaacggtc catggtcaaa
     2881 taataaaaac ggacgacctt atcgagcaac ccgggaagca cggcgaccat cacggccagg
     2941 atggccaggg tgaccaacac ttcaataaaa gtgaatccgc cggagcgacg gaagcgcgat
     3001 cgacggcgcg tcacggggtc aaatccagga ccgggaaatc cgtggcgttg ttgcccgaat
     3061 tgatgaaggc ggaaatggaa cgcttgaccc aggtcacgtc cccggcggtg ttgaagtagg
     3121 ggtagaaacc gtaaacgttg taggtcccca ttttcacgtc aaaagtgtag cgcccagcgg
     3181 cgttggacgc ggtcatgtag tattcgagac cgcccgcccg gaattgcgag gtg
//
LOCUS       DAOQLS010000173         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16211_length_3230_cov_4.067402, whole genome shotgun sequence.
ACCESSION   DAOQLS010000173 DAOQLS010000000
VERSION     DAOQLS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16211_length_3230_cov_4.067402"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PK362_04310"
     CDS             complement(<1..494)
                     /locus_tag="PK362_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HNC74092.1"
                     /translation="MLLIQLSALALLLAVSAYFALAESAITALSALRMKRLALLAPTL
                     RSYFHDWLSHPHRLLSILMLGNNFVNFGFSSLAAVVALPLYHRWPRPVVNVAVWWVVT
                     AFLLVGGEIVPKIIGRAYRERVAEMTLPLLSRLTRALFIVWGPLGWLIQRFAPTLNRA
                     PVNLL"
     gene            complement(502..963)
                     /gene="ybeY"
                     /locus_tag="PK362_04315"
     CDS             complement(502..963)
                     /gene="ybeY"
                     /locus_tag="PK362_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNC74093.1"
                     /translation="MNIVCSTLGARRPPAEARAVRGARAALRHGRARGDELHIVWTTR
                     ARLRILNRRFRQKNRFTDVIAFRYPAERGRSPFDGPAAGAPFGDLYIATDQARLNARR
                     FGSTRDEEIVRLATHGTLHLLGYTDYEPAPRARMWAVQEPIVRRIMGARSR"
     gene            complement(960..2492)
                     /locus_tag="PK362_04320"
     CDS             complement(960..2492)
                     /locus_tag="PK362_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNC74094.1"
                     /translation="MSHLFTQFVSHTLRPLLLRLLRWTERSGPLSAPRSSLFLHREIH
                     VPVWAVGAVVWVMLSTVLFLEIGPGWPQAAAVLILSTALIALFGLYLRFDHPELAQDE
                     DAVAFLGVLIVGSVLGMELWFDAVQHFSWISPFGFPLPLAGLLAAILLHPRLAVISTV
                     VLSLVFGILNGFSLESSLVVCFAGMAGVSRALRVRTRGDITRAGLWVALGGAAAVGML
                     AMMERWPRAQTLFAIKWVGVGSALSTLLTLGLLPYMELFFSRLSNIRLLELSDVNHPL
                     LKRLSLEAPGTFHHSLIMASLAQAAAERIGANALLCRVGAYFHDIGKLAKPEYFVENQ
                     GALGNPHELLPPNMSRIVIQSHVKDGLALAQRHNLDRTLMDFITMHHGTSRIEYFYRR
                     AIERAGAEDVDEDIYRYPGPRPLTRETAIVMLADSVEASTRTVENPTHQRLADHVARI
                     VESKINDGQFDHVPLTLAEIQQVKDSFVNTLVGVYHTRIRYPTSQETDVEIRAAAPAP
                     KK"
     gene            complement(2507..>3230)
                     /locus_tag="PK362_04325"
     CDS             complement(2507..>3230)
                     /locus_tag="PK362_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNC74095.1"
                     /translation="PPNKYPPDSVYVTVTGAAIRPQTPSQQAYVKAIRDNDMVFGVGP
                     AGTGKTYLAVACAIAALESGRVNRIVLTRPVVEAGEKLGFLPGDFYEKVHPYLKPLYD
                     AFHNMVGADRFRQLREDETIEIIPLAYMRGRTLDEAFAILDEAQNTTPEQMKMFLTRM
                     GNGSRMVITGDVTQIDLDKKLTSGLVQIQDVLRGVEGIDFVRFSEVDVVRHPLVKKII
                     RAFDAWDERGRTPPPGSPPPAV"
BASE COUNT          623 a          976 c         1148 g          483 t
ORIGIN      
        1 agcaaattga cgggggcccg gttgagcgtc ggggcgaacc gctggatcaa ccaccccagg
       61 ggcccccaga cgatgaacag ggcgcgggtc aggcggctga gaagcggcag ggtcatttcg
      121 gccacccgct cccggtaggc gcggccgatg attttgggga cgatttcccc cccgaccagc
      181 aaaaacgcgg tcacgaccca ccagacggcg acgttcacga cggggcgggg ccaccggtga
      241 tacaagggca gggcgaccac cgccgccagg ctcgagaatc cgaaattgac gaagttgttg
      301 ccgagcatca agatcgagag aagccggtgg gggtgggaaa gccaatcgtg gaaataagac
      361 cggagcgtcg gcgccagaag cgccaggcgt ttcatgcgca gggccgacag ggcggtgatg
      421 gccgactcgg ccagggcgaa ataggcggac acggccaaca acagggccag ggccgacagc
      481 tggatcaaca acacgggcgg ttcagcggga ccgggcgccc atgatccggc ggacgatggg
      541 ctcctggacg gcccacatgc gcgcccgggg ggcgggctcg tagtcggtgt agccaagcaa
      601 atggagcgtc ccgtgggtgg ccagccgaac gatctcttcg tcccgggtcg acccgaaccg
      661 ccgggcgttc aaccgggcct ggtcggtggc gatgtacagg tcgccgaagg gcgccccggc
      721 ggcggggccg tcgaaggggg accgaccgcg ctccgcgggg tagcggaaag cgatgacgtc
      781 ggtgaaccga ttcttttggc ggaagcggcg gttgaggatc cgaaggcggg cgcgggtggt
      841 ccacacgatg tggagttcgt cgccccgggc ccggccgtgg cgcagggccg cgcgggctcc
      901 tcgaacggcc cgggcttcgg cgggcgggcg ccgggccccc agggtggagc aaacgatgtt
      961 cactttttgg gggccggcgc ggcggcgcga atttcgacgt cggtttcctg ggacgtggga
     1021 taacggatcc gcgtgtggta gacgccgacc agggtgttca caaaactgtc cttcacctgc
     1081 tggatttccg cgagggtcag gggcacgtgg tcgaattggc cgtcgttgat tttggattcc
     1141 acgatgcgcg ccacgtggtc cgcgagccgc tgatgcgtgg ggttttccac ggtccgggtg
     1201 gaggcctcca ccgaatcggc gagcatgacg atggccgttt cccgggtcag gggccgggga
     1261 cccgggtagc ggtaaatgtc ctcgtccacg tcctcggcgc ccgcccgttc gatggcccgg
     1321 cggtagaaat actcgatgcg cgaggtgccg tggtgcatgg tgatgaaatc catgagcgtg
     1381 cggtccaggt tgtggcgctg ggcgagggcc aacccgtcct tcacgtggga ttgaatgacg
     1441 atgcgggaca tgttcggcgg caggagctcg tgggggttgc ccagggcgcc ctggttctcc
     1501 acgaaatatt ccggtttggc caacttgccg atgtcgtgaa agtaggcgcc cacccggcaa
     1561 agaagcgcgt tggccccgat gcgctccgcg gccgcctggg ccagggacgc catgatgagc
     1621 gagtgatgga acgtcccggg ggcctccagg ctcaaccgct tcaacagcgg gtgattgacg
     1681 tcggaaagct ccagaagacg gatgttggac aaccgggaga aaaacaattc catatagggc
     1741 aggagcccga gggtcagaag cgtggaaagg gcgctgccca cgcccaccca tttgatggcg
     1801 aatagcgtct gggcccgggg ccaacgctcc atcatcgcca gcatccccac cgcggccgcc
     1861 ccgcccaggg ccacccaaag gcccgcccgg gtgatgtcgc cccgggtgcg gacccgcaga
     1921 gcccgggaaa cgcccgccat accggcgaaa cacacgacca gggaggattc cagggaaaac
     1981 ccgttgagaa tgccgaacac gaggctcagg acgaccgtgg agatcacggc caggcgcggg
     2041 tgaagcagga tggcggccag caggcccgcc aacggcagcg ggaagccgaa gggcgaaatc
     2101 caggaaaaat gttgcaccgc gtcgaaccac aactccatgc ccagcacgct gcccacgatc
     2161 aacacgccca ggaaagccac cgcgtcctcg tcctgggcca actcggggtg gtcaaaacgg
     2221 aggtaaaggc cgaacagggc gatcaaggcc gtcgaaagga tcaacaccgc cgcggcctgg
     2281 ggccacccgg ggccgatctc cagaaacaat accgtcgaca acatgaccca cacgaccgcg
     2341 cccacggccc acaccggcac gtggatttcc cggtgcagga aaagggagga ccgcggcgcc
     2401 gacaggggcc cggaccgctc cgtccaacgc agaagacgca ggaggagggg acgaagggtg
     2461 tggctgacga attgggtgaa caggtggctc atggaatcgg agcgctttaa acggccgggg
     2521 gcggcgaacc ggggggcggg gtccggcccc gctcgtccca ggcgtcaaag gcccggatga
     2581 tcttcttgac caaggggtgc cgcaccacgt ccacttcgga gaaacgcaca aaatcgatgc
     2641 cttcgacgcc ccgaaggacg tcctgaattt ggacgagacc cgaggtcaac tttttgtcca
     2701 agtcgatctg ggtcacgtcg ccggtgatga ccatccgcga cccgtttccc atgcgggtca
     2761 ggaacatttt catttgctcg ggggtggtgt tctgggcttc gtccagaatc gcgaaggctt
     2821 cgtccagggt ccggccccgc atgtaggcca ggggaatgat ttcgatggtt tcgtcctccc
     2881 gcagctggcg aaaacggtcg gcgccgacca tgttgtgaaa ggcgtcgtag aggggtttca
     2941 ggtaggggtg caccttttcg taaaaatccc cgggaagaaa tccgagtttc tcccccgcct
     3001 ccacgaccgg gcgggtcagg acgatgcggt tgacccggcc gctctcgagg gccgcgatgg
     3061 cgcaggccac ggccaaatag gttttgccgg tcccggccgg accgacgcca aacaccatgt
     3121 cgttgtcccg gatggctttc acgtaggcct gctgggaggg ggtctgggga cgtatggccg
     3181 ccccggtcac cgtgacgtag acggagtccg gcgggtattt gttgggcggc
//
LOCUS       DAOQLS010000174         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16242_length_3227_cov_4.313052, whole genome shotgun sequence.
ACCESSION   DAOQLS010000174 DAOQLS010000000
VERSION     DAOQLS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16242_length_3227_cov_4.313052"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK362_04330"
     CDS             <1..327
                     /locus_tag="PK362_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74096.1"
                     /translation="GRAFFALDRLEEAGRDFDKALLLDPAMAEARLNRGQTFMNRRRF
                     PEAALDFAEATRRHPEWTAAWIKLGLARRLAGDLPGAAEAFRRALVLDPGLDSVRRWL
                     AELPAT"
     gene            373..3189
                     /gene="ileS"
                     /locus_tag="PK362_04335"
     CDS             373..3189
                     /gene="ileS"
                     /locus_tag="PK362_04335"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNC74097.1"
                     /translation="MKPPKFSVHLPKTNFPMKADLPLREPATQSHWEKIHLYKKMQDR
                     AAEKQSPAFVLHDGPPYANGAIHVGHALNKILKDVIVKYKAMKGFRAPYIPGWDCHGL
                     PIEHQLMKEKGWDKRKVGRLAFRQEATRYAEHWIDLQRRDFKRLGVLADWDHPYKTLA
                     PEYEAAVAETFYKLFETGGVVQDKKPVHWCPSCETALAEAEIEYENKTSPSIFVKFPI
                     TEWPSNDDAQSLAKTPGKKPTVLVWTTTPWTLPANVALAFHPDHTYRLWESAKTGERF
                     LVGDPGVAVLEEVLGPGKAVAVEILGESLEGLVAKNPINNNDSQGVLAKFVSNTDGSG
                     VVHIAPGHGEEDYVVGRSYGLPVLSPVDDAGRFTADVLPNDLAGKTVWEANPLIIERL
                     TKSLLLLKTAPVTHSYPHCWRCKNPIIFRAALQWFLKVTDEFRAQLIESTERVRWIPE
                     YGQERILGMLKSRPDWCLSRQRYWGTPIPMFACVKCNEPLNDKKVFAAVVEAFRQKGG
                     DVWYEKDPAFFGAKGPCAKCGGTAFKKEEDILDVWFDSGVSWAAVINSRPETRGTPRA
                     DVMYLEGSDQHRGWFQTSLLPSVRLASEPPYGQVLTHGFVLDGQGRAMSKSLGNVIAP
                     QALIEKYGADVLRLWVAVTDYREDVRLSQDILDRVIDTYRKIRNTLRFLLGNLGDFVP
                     AKHAVPLQKMEPLDLAALTALKKLIEQTATHYDQSEFHQVAGALADQFCINALSEYYL
                     DVRKDILYCDLADSPRRRSAQTAFWHIARALAKLLAPILSFTAEETWQTLREQNLLDA
                     SDNPESVFLNSFPAAPTAPPTTTIADYLLARRLINEAVEKARQSGAVKGMNDALVHLK
                     FGVPGPNFDGFVKTDAELASVLGVAALRTEKTTGPTTEESVSLAPGAKCPRCWLWREL
                     GPAGLCVRCTEAEAQAAQAAPAPR"
BASE COUNT          624 a         1086 c          949 g          568 t
ORIGIN      
        1 ggacgcgcct ttttcgccct ggaccgactg gaggaggcgg gccgggactt cgataaagcc
       61 ctgctcttgg acccggccat ggccgaggcg cggctcaacc gcggccaaac tttcatgaac
      121 cggcggcgat ttcccgaggc cgccttggat ttcgccgagg cgacccgccg tcatcccgag
      181 tggaccgcgg cctggatcaa attggggttg gcccggcgtc tggccgggga tttgcccgga
      241 gccgccgagg cctttcggcg ggccctcgtc ctggacccgg gcctggattc cgtccgccgc
      301 tggctggccg aactgcccgc cacttagacc ctcgggtttc gttcccttcg ccgatcagct
      361 acaatagtcg ccgtgaaacc cccgaagttc tccgtccatc tgcccaagac gaatttcccc
      421 atgaaagcgg acctccccct ccgggagccc gccacccagt cccattggga aaaaatccat
      481 ctttacaaaa agatgcagga ccgggcggcc gagaagcaat ccccggcctt tgtccttcac
      541 gacggcccgc cctacgccaa cggggccatt cacgtgggcc acgccctgaa caaaatcctg
      601 aaggacgtca tcgtgaaata caaggcgatg aagggatttc gcgcccccta cattcccggg
      661 tgggactgcc acggcttgcc catcgagcat caattgatga aggaaaaggg ctgggacaaa
      721 cgcaaggtgg gtcgcctggc cttccgtcag gaggccacgc gctacgcgga acattggatc
      781 gacctccagc gccgggattt taaacgactg ggggttttgg ccgactggga ccacccctac
      841 aagaccctgg ctcccgagta cgaggccgcc gtggccgaaa ctttttacaa actcttcgag
      901 acgggcggcg tcgttcagga caaaaaaccc gtccactggt gcccctcctg cgaaacggcc
      961 ctggcggagg ccgagatcga gtatgaaaac aaaacgtcgc cctcgatctt cgtcaagttt
     1021 cccatcaccg agtggccgtc caacgacgac gcccaatccc tggccaagac ccccggcaaa
     1081 aagcccacgg tgctggtttg gacgaccacg ccctggacgc tcccggccaa cgtggccctg
     1141 gcgttccacc cggaccacac ctaccgcctt tgggaatcgg ccaaaaccgg cgagcgcttc
     1201 ctggtggggg accccggcgt ggccgtgctc gaggaagttc tggggcccgg caaagccgtg
     1261 gcggtggaaa ttctgggcga atctctcgaa gggctcgtgg ccaaaaaccc catcaacaac
     1321 aacgacagcc agggcgtttt ggccaagttt gtctccaaca ccgacgggtc gggcgtggtg
     1381 cacatcgccc cgggccacgg cgaagaggac tacgtcgtgg gccggtccta cggattgccc
     1441 gtgctctcgc ccgtggacga cgccggccgc ttcacggccg acgtgttgcc caacgacttg
     1501 gccggaaaaa ccgtctggga ggccaacccc ctcatcatcg aacggctgac caaatccctt
     1561 ttgctgttga aaaccgctcc cgtcacccac agctaccccc actgctggcg gtgcaaaaac
     1621 cccattatct tccgcgcggc gctccagtgg ttcctcaaag tcaccgatga attccgcgcc
     1681 cagctcatcg agagcaccga gcgggttcgc tggatccccg agtacggcca agagcgtatt
     1741 ttgggcatgt tgaaatcccg gcccgactgg tgcctttccc gccagcgcta ctgggggacg
     1801 cccatcccca tgttcgcctg cgtcaaatgc aacgagcccc tcaacgacaa aaaagtcttc
     1861 gccgccgtcg tggaggcctt ccgccaaaag ggcggcgacg tgtggtacga gaaagacccg
     1921 gccttcttcg gcgccaaggg cccctgcgcc aaatgcggcg gcacggcttt caaaaaagaa
     1981 gaggatattt tggacgtgtg gttcgactcc ggcgtgtcct gggccgccgt gatcaacagc
     2041 cgtcccgaaa cccgggggac cccgcgcgcc gatgtgatgt atctggaagg ctccgaccaa
     2101 caccgggggt ggttccagac gtcgctcctc ccgtcggtgc ggctcgcgag cgagccgccc
     2161 tacggccagg tgctgaccca cgggttcgtc ttggacggcc aggggcgggc catgtccaaa
     2221 tccctcggca acgtcatcgc gccccaggcc ctgattgaaa aatacggcgc cgacgtgctg
     2281 cgtctctggg tggcggtcac ggactaccgg gaggacgtgc gcctttccca ggacatcctg
     2341 gaccgggtga tcgacaccta ccgaaaaatc cggaacaccc tgcggttcct cctcgggaac
     2401 ctgggcgatt ttgtcccggc caagcacgcg gtgcctctcc aaaaaatgga gcccctggat
     2461 ttggcggcgc tcaccgccct gaaaaaattg atcgagcaaa ccgcgacgca ttacgaccaa
     2521 agcgagttcc accaggtcgc cggcgctctg gcggaccagt tctgcatcaa cgccctttcg
     2581 gagtattatt tagacgttcg taaagatatt ctttattgcg acctcgccga cagcccccgt
     2641 cgacgaagcg cccaaacggc tttctggcac atcgcccggg ccctggccaa gctgctggcc
     2701 cccattttgt cgtttaccgc cgaggaaacc tggcagaccc ttcgggaaca gaatttattg
     2761 gacgcgtcgg acaaccccga gagcgttttt ctaaattcat tcccggccgc gccgaccgcc
     2821 ccccccacga cgacgatcgc cgactattta ttggcccgaa ggttgatcaa cgaggcggtg
     2881 gaaaaggccc gacagtctgg cgccgtcaaa ggaatgaatg acgccttggt gcacctgaag
     2941 ttcggcgtgc cgggcccaaa tttcgacggg tttgttaaaa cggacgccga gctggcttcg
     3001 gtgttggggg tcgcggccct tcggacggaa aaaacaacgg gaccgacgac cgaggagtcg
     3061 gtttccctcg cccccggcgc caagtgcccc cgctgttggc tgtggcgtga attgggtccc
     3121 gcgggcctct gcgtccgttg caccgaggcc gaagcccaag cggcccaggc cgcccccgcg
     3181 ccccggtgaa aaagtccgcc gccctcactc tcggggttct ggtcctg
//
LOCUS       DAOQLS010000175         3221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16303_length_3221_cov_2.861339, whole genome shotgun sequence.
ACCESSION   DAOQLS010000175 DAOQLS010000000
VERSION     DAOQLS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16303_length_3221_cov_2.861339"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PK362_04340"
     CDS             complement(<1..648)
                     /locus_tag="PK362_04340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC74098.1"
                     /translation="MIGAALTVGALAAAGFSARWNWWRRRAPGLPVLMYHKVGTPPPG
                     SKLKKLWVSPAQFRWQMSYLKRHGYAPLTLADVAAHQDAGRPVPANGVVLTFDDGYRN
                     NLENALPILQEFGFPAVIYVVVNAVGRDNFWHDPASETRIPMLDWDGVRALQKAGWEI
                     GSHTMNHARLSRLSADAVRAELVESRSALAEKLGTPPISFANPYGDAADDPAVQSL"
     gene            complement(645..1394)
                     /locus_tag="PK362_04345"
     CDS             complement(645..1394)
                     /locus_tag="PK362_04345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC74099.1"
                     /translation="MPRLSAILIARNEERDLPACLESLRGVADEIVLVDSHSTDRTRD
                     IARAHGARVVERDFAGFGPQKQFALEQATGDWVLNIDADERLTPALAEDIRRALASGP
                     TVNGYELPFHVFFLGRRLRFGGCFREFHLRLFRRDKAHYAGAEIHEGISVPRPWGRLP
                     HAVRHESYRNFQEYLEKCARYTGLIARKKYAEGRRFAFWQHLRLPWEFAVRYVFKGGF
                     LDGNAGLIYAVLSSYYAWLKHVRLLDGGDNA"
     gene            complement(1382..2482)
                     /locus_tag="PK362_04350"
     CDS             complement(1382..2482)
                     /locus_tag="PK362_04350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC74100.1"
                     /translation="MSFRILHLNTERGWRGGERQTLWLARELERRGHVNILAARPKEP
                     LARAAAEAGLRVFPLAPWSEWDFRNAARLRVFLRQERLDLVHAHTGHAVGLGALARRG
                     TAARLVATRRVDFPLKNNFLSRWKYRSVDALAAISDAVRQMLVRGGVLPSKITVIPSG
                     VDTGAYPDARDRDRLRSERGFSSRDRLVVHAGALVPHKDQATLLRAFAGLRARGAADR
                     LLVLGDGPRREELVRLSETLGLGGAVAFLGHRGDVLEYTAMADLFVFSSVDEGLGTAL
                     LDALAMGVPTAATAAGGIPDLYGGPGAPELSAPGDADALAANMAAVLENPEEARRRSE
                     RGRERARAFTVSAMADAYETLYRKLLEAPCPA"
     gene            complement(2479..>3221)
                     /locus_tag="PK362_04355"
     CDS             complement(2479..>3221)
                     /locus_tag="PK362_04355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC74101.1"
                     /translation="RRLAPRPGQGFLFRRTDAVIAASRALGEAYAAAYPFLRERTRVV
                     FPGVALSPATAEPPAPLRTAVVGRLDPVKGHAYFLEAVERLRDRGPVDEFWIVGEDKN
                     LTRAELEKEAGRRGVADRVRFAGRLPDVAAFMRSCHLGVVSSVGSEAVSRVALEWLAL
                     GRPIVATRVGALPELVEDGKNGRLVPPRDGAALAAALGALLDDGEARRRMGAEARRRV
                     EHQFSVPRWGEETEAAYRRAVDHRRRPT"
BASE COUNT          547 a         1130 c         1082 g          462 t
ORIGIN      
        1 gagcgactgc acggcggggt cgtccgcggc gtcgccgtag gggttggcga aggagattgg
       61 cggcgtcccc agtttttcgg ccaacgccga ccggctttcg accagctcgg cccggaccgc
      121 gtcggcggac aggcgggaga gccgcgcgtg attcatcgtg tgggaaccga tttcccatcc
      181 ggccttttga agggcccgaa cgccgtccca atcgagcatg gggatccgcg tttccgaggc
      241 cgggtcgtgc cagaaattgt cccggcccac ggcgttcacc accacgtaga tcaccgccgg
      301 aaaaccgaat tcctggagaa tcgggagggc gttctccaaa ttgttccggt agccgtcgtc
      361 aaaggtgagc acgacgccgt tggccggcac gggacgcccg gcgtcttggt gagccgcgac
      421 gtcggccagg gtgagcgggg cgtacccgtg tcttttcaaa tacgacatct gccaacggaa
      481 ttgcgccggg gacacccaaa gctttttcag cttggacccc gggggcggcg tccccacttt
      541 gtggtacatc aggaccggca ggcccggggc ccggcggcgc caccaattcc agcgcgcgga
      601 gaaccccgcg gcggccaggg cgccgacggt caacgccgcg ccgatcacgc gttgtccccg
      661 ccgtccaaga gtcggacgtg cttcaaccag gcgtagtagg aactcaaaac ggcgtagatc
      721 aaaccggcgt ttccgtccaa gaatccccct ttaaacacat aacgcaccgc aaattcccag
      781 ggcaaacgga ggtgctgcca gaaggcaaag cgccggccct cggcgtactt tttgcgggcg
      841 atcagccccg tgtagcgggc gcatttctcc agatactcct ggaaattgcg gtagctttcg
      901 tggcgcacgg cgtgggggag gcgtccccag gggcgcggca ccgagatgcc ctcgtggatt
      961 tcggccccgg cgtaatgggc cttgtcccgg cggaaaaggc gcaggtgaaa ctcgcggaaa
     1021 caccccccga accgcaaacg ccgtccgagg aagaacacgt ggaagggcaa ctcgtacccg
     1081 ttcaccgtcg gcccggacgc cagcgcccgg cgaatgtcct ccgcgagggc cggcgtgagc
     1141 cgctcgtcgg cgtcgatgtt gagcacccag tcgccggtgg cttgctcgag agcgaactgt
     1201 ttctgggggc cgaacccggc gaaatcccgc tccaccaccc gggcgccgtg ggcccgggcg
     1261 atgtcccggg tgcggtccgt ggaatggctg tccaccaaaa cgatttcatc ggccaccccc
     1321 cgaaggcttt ccaggcaggc gggaaggtcc cgctcctcgt tgcgggcgat caggatggcg
     1381 ctcaggcggg gcacggggcc tccaacagtt ttcgatacag cgtctcgtac gcgtcggcca
     1441 tggcggacac cgtaaaggcg cgggcccgtt cccggccgcg ctccgaccgg cggcgggctt
     1501 cctccgggtt ttccaaaacc gcggccatgt tcgcggccag ggcgtcggcg tcgcccgggg
     1561 cggaaagctc cggcgccccc gggcccccgt agaggtcggg gattcccccg gcggcggtgg
     1621 cggccgtggg cacccccatg gccagcgcgt ccaaaagagc cgtgccgagg ccttcgtcca
     1681 ccgaagagaa cacaaagaga tccgccatgg ccgtatattc taacacgtcg ccgcggtgcc
     1741 cgaggaaagc cacggccccg cccaggccaa gggtttcgga caggcgcacg agttcctccc
     1801 gccggggccc gtcccccaga accagcagac ggtcggccgc gccccgggcg cgaagcccgg
     1861 caaaggctcg aagcagggtc gcctggtctt tgtgggggac cagggcgccc gcgtggacca
     1921 ccaaacggtc ccgggacgag aaaccgcgct cggaacgaag gcggtcccgg tcccgggcgt
     1981 cggggtaggc tccggtgtcc acgccgctcg gtatgaccgt gattttggac gggagcacac
     2041 cgccgcgaac cagcatttgc cgcaccgcat cggagatggc cgccaaagcg tccaccgacc
     2101 ggtatttcca ccgactgaga aagttgtttt tcaaggggaa gtccacccgg cgggtggcga
     2161 ccaatcgggc ggcggtgccg cgccgcgcca gggcccccag gcccacggcg tggccggtgt
     2221 gggcgtgaac gaggtccagg cgttcttgtc gcaggaaaac ccggagtcgc gccgcgtttc
     2281 ggaaatccca ttccgaccag ggcgccaggg gaaagacccg caaccccgcc tccgccgccg
     2341 cccgggccag gggctccttc ggccgggccg ccaggatatt gacgtgcccc cggcgttcca
     2401 attcccgggc gagccacagg gtttgacgct ccccgccccg ccagccccgt tcggtgttca
     2461 aatgaaggat tcgaaaactc acgtcggcct ccgacgatgg tccaccgccc ggcggtaggc
     2521 ggcctcggtt tcctcccccc aacgggggac cgaaaattga tgttcgacgc ggcgccgggc
     2581 ttccgcgccc attcgacggc gggcctcgcc atcgtcgagc aatgccccca gggccgccgc
     2641 caaggccgcc ccgtcccggg gcggaaccag tcggccgttc tttccgtcct ccacgagttc
     2701 cggcagcgcg cccacccgcg tggccacgat cggccgtccc agggccagcc attccagggc
     2761 gacgcgggac accgcctcgc tgccgacgga ggaaaccacc cccagatggc aggaacgcat
     2821 gaacgccgcc acgtcgggga gtcgtccggc gaagcgcacc cgatcggcga cgccccgccg
     2881 cccggcctct ttttccaatt cggcacgcgt caggtttttg tcctcgccga caatccaaaa
     2941 ttcatccacg ggcccgcgat cccgcagtcg ctcgacggct tccaaaaaat acgcgtggcc
     3001 cttgacgggg tcgaggcggc ccaccacggc cgtccgcagc ggggcgggcg gttccgccgt
     3061 cgccggtgaa agggccaccc cgggaaacac cacccgcgtt cgttcccgca aaaagggata
     3121 agccgccgcg taggcttcgc ccagggcgcg ggaggcggcg atgacggcgt cggtgcgacg
     3181 aaacagaaac ccctggccgg ggcgcggggc caggcgccgg g
//
LOCUS       DAOQLS010000176         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16320_length_3218_cov_4.164401, whole genome shotgun sequence.
ACCESSION   DAOQLS010000176 DAOQLS010000000
VERSION     DAOQLS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16320_length_3218_cov_4.164401"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1697)
                     /locus_tag="PK362_04360"
     CDS             complement(36..1697)
                     /locus_tag="PK362_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC74102.1"
                     /translation="MSKNGPKKRLGDILIQEGLITAIQLERAVEAQRTKGGKLGDLLI
                     GLGFCTEEDVLSVLSKRSGYGYLTALSTRPAVPPAVLAHIPAALAWSKNLVPVAKEGN
                     VLSVAVADPFAEFDSIGELKIQTGCDIKLVLASESEIQKAVARYYPDRKDVVAAADAG
                     PALEAMDTVIGALVENAAKAGADHIFLEPGAQALHVRYRSKAGLQSRPDIPLAHQASL
                     VARLKTLARLNVGECWVPQVGRLRWTASGRPMDLTVTTLPTSLGEKIVFKLVDADRAM
                     PLDLEKLGLEGESRSQFEKMLELKKGLILAVGPTGSGKSSTLHAALARLSDPSRHLVS
                     VEDPVERYLDGVSQLQARPDARLTLASGVHILRRLGPDVLYVSDLRDLPTAEAALQAS
                     SECLVLAALTASSALAGIERLWELGVSAAQLAQALVGVVGQRLVRTVCPHCREPHTFS
                     LRQLMAQGVGSQEMRAAKRADSFTLYRGRGCGQCLGTGFLGAAAVFELLPATDGVRRL
                     IWEKAGRAALARETSERVTLREAAVNKALAGLTTIDEALRVGDQS"
     gene            complement(1712..2761)
                     /locus_tag="PK362_04365"
     CDS             complement(1712..2761)
                     /locus_tag="PK362_04365"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNC74103.1"
                     /translation="MTAIHPRVILEKHGEKSPGTSPFWEDFRRRSTAKSRRILLPEAT
                     DPRVLSAAIFLKRVGIARPVLVGDPVTVRAAAADGGWSLDGIEILDSTNAADRAAFAQ
                     ALFERRKAKGTTAEEAARLVEDPLYWSAMALAGGRADGLVGGAVRTTADTVRAGFAGV
                     GLAHGADIVFGAFFMECPHATGGARRLMFADAAVSPHPSPRALGAIGVAAAEFWRRQT
                     GETPRVAYLSFSTLGSAEDESVTAVRQAVAHAKKKAPDLSVDGEWQADAALVDHIARQ
                     KGVPDGPAAGRANVMIVPDLNAGNIGYKMVQHLGGARAVGPFLVGLGKPMADLSRGCT
                     DEDIVDTVAVTSLLD"
     gene            complement(2794..>3218)
                     /gene="coaD"
                     /locus_tag="PK362_04370"
     CDS             complement(2794..>3218)
                     /gene="coaD"
                     /locus_tag="PK362_04370"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNC74104.1"
                     /translation="RHIFDHVIVAVTQNSAKNSLFTVQERVDLLSAALKKEIRSRSVS
                     VDTFSGLLVDYCRRRRAVAVARGLRALSDFEYEFQMALMNRHLAPKLETVFLMPDEKY
                     TYLSSTLLKEIVRLGGDVGRFVPAALVPKIRQKLAGRG"
BASE COUNT          578 a         1083 c         1021 g          536 t
ORIGIN      
        1 gatcgttcat gctgatcatg gcagatcccc cgtgttcaac tttggtcgcc gacccgcaac
       61 gcttcgtcga tggtcgtcag tcccgccagg gccttgttca ccgcggcttc gcggagcgtc
      121 acccgttccg aggtttcccg cgccaacgcg gcgcgtcccg ctttttccca aatcaatcgc
      181 cggacaccgt ccgtcgccgg caacagttcg aagaccgcgg cggcgcccaa gaaacccgtc
      241 cccaggcact ggccgcaccc ccgcccccgg tacaaggtga aggagtcggc ccgtttggcg
      301 gcccgcattt cctggctgcc gacgccctgg gccatcaatt ggcggagaga aaaagtgtgg
      361 ggttcccggc aatgggggca cacggtccga accagcctct ggccgaccac cccaacgagg
      421 gcctgggcca gttgggcggc cgacacgccg agctcccaca accgctcgat gcccgccagc
      481 gcggacgacg cggtcagggc cgccaacacc aggcattccg acgacgcctg cagggcggcc
      541 tcggcggtgg gcaaatcccg caaatccgag acgtagagga cgtcggggcc caaccgtcgc
      601 aggatatgaa ccccggacgc caacgtcaaa cgagcgtccg gccgcgcctg aagctgggag
      661 acgccgtcca gataacgttc gacgggatct tcgacggaaa ccaagtggcg ggagggatcg
      721 ctcaggcggg ccagggccgc gtgcagggtg ctgctttttc cggagcccgt gggacccacc
      781 gccaggatca agcccttttt caactccaac attttttcaa attgcgaacg ggattccccc
      841 tccagcccga gtttttccaa atccaagggc atggcgcggt ccgcgtcgac cagtttgaaa
      901 acgatcttct ccccgaggga ggtcggcagg gtcgtcaccg tcaggtccat ggggcgtccc
      961 gaggccgtcc atcgcaggcg tccgacctgc ggcacccaac actcccccac gttgaggcgg
     1021 gccagggttt tgagccgggc gacgagcgac gcctgatggg ccagcggaat atcgggccgg
     1081 gactggagac cggctttgct tcggtaacgg acgtgcagcg cctgggcgcc gggctcaaga
     1141 aaaatatggt cggccccggc tttggccgcg ttctccacca aggcgccgat gaccgtgtcc
     1201 atggcctcca gcgccggccc ggcgtccgcc gccgcgacga cgtctttgcg gtcggggtaa
     1261 taacgggcga cggctttttg aatttccgat tccgaggcga ggaccagctt gatgtcgcac
     1321 ccggtttgga ttttaagctc cccgatggaa tcgaactcgg cgaagggatc ggccacggca
     1381 acggaaagga cgttgccctc cttggcgacg ggcaccaaat ttttggacca ggccaaggcc
     1441 gcggggatgt gggccaacac cgcgggcggg accgcggggc gggtggacag ggccgtgagg
     1501 tagccgtagc ccgatcgttt ggacaaaacc gacaacacgt cctcttccgt gcagaacccg
     1561 aggccgatca gcagatcgcc cagcttaccg cctttcgtgc gctgggcctc caccgcccgt
     1621 tccaattgaa tggcggtgat gaggccttcc tggatcagga tgtcccccag gcgttttttg
     1681 ggaccgtttt tcgacatgag ccctcttttc gtcagtccaa aagcgacgtc accgcgacgg
     1741 tgtccacgat gtcttcgtcc gtgcagcccc gggacaagtc cgccatgggt ttgcccagcc
     1801 ccaccagaaa ggggcccacc gcccgggcgc cgcccaaatg ctgcaccatt ttgtagccga
     1861 tgtttccggc gttcaaatcg ggcacgatca tcacgttggc ccggcccgcg gcggggccgt
     1921 cgggcacgcc cttctgccgg gcgatgtggt ccaccagcgc ggcgtccgcc tgccattcgc
     1981 cgtccaccga aagatccggg gcttttttct tcgcgtgagc caccgcttgc cggaccgcgg
     2041 tcaccgactc gtcctcggcg ctccccaggg tggaaaaact caaataggcg acccgggggg
     2101 tttcgcccgt ctggcgtcgc cagaattccg cggcggcgac gccgatggcg cccagggccc
     2161 ggggcgacgg atggggcgac acggcggcgt ccgcgaacat caggcggcgg gccccgcccg
     2221 tcgcgtgggg gcattccatg aaaaacgccc cgaaaacgat atcggccccg tgcgcgagcc
     2281 ccacccccgc gaaccccgcc cggaccgtgt ccgccgtggt acgaaccgcg ccccccacga
     2341 gaccgtcggc ccgtcccccg gccagggcca tcgcggacca ataaagcggg tcttcgacga
     2401 gacgggccgc ttcctcggcc gtggttcctt tcgctttgcg tcgctcaaag agcgcctggg
     2461 cgaaagcggc ccggtcggcc gcgttcgtcg aatccaaaat ttcgatcccg tcgagggacc
     2521 agcccccgtc cgccgccgcg gcgcggacgg tcaccgggtc ccccaccaac accggccggg
     2581 caatcccgac gcgcttaaga aaaatggccg cggacaatac ccgcgggtcc gtcgcctcgg
     2641 gcaggagaat ccggcgcgac ttcgcggtcg aacgtcgacg gaaatcttcc caaaagggcg
     2701 aggttcccgg cgatttctcg ccgtgttttt caagaataac gcggggatgg atcgctgtca
     2761 agggaagtcc ccgggcgccg cccgcgggcc cctttaaccc cgtcccgcca acttttggcg
     2821 gattttgggc acgagcgcgg cggggacaaa ccgccccacg tcgccgccca atcgcacgat
     2881 ctcttttaaa agggtggagg agaggtaggt gtatttctcg tcgggcatca ggaacacggt
     2941 ttcgagcttg ggcgccaaat ggcggttcat gagggccatt tgaaattcgt attcaaaatc
     3001 cgacagggcc cgcaggcccc gggccacggc caccgcccgg cgccgccgac aataatccac
     3061 gaggagaccc gaaaaagtgt ccacgctgac ggaccgggat cggatttcct ttttcaaggc
     3121 ggcggaaaga aggtcgacgc gttcttgaac ggtaaaaagg gaattttttg cgctgttttg
     3181 ggtgaccgcc acgatcacgt gatcaaagat gtggcggg
//
LOCUS       DAOQLS010000177         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16392_length_3210_cov_4.797147, whole genome shotgun sequence.
ACCESSION   DAOQLS010000177 DAOQLS010000000
VERSION     DAOQLS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16392_length_3210_cov_4.797147"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1027)
                     /gene="rfbA"
                     /locus_tag="PK362_04375"
     CDS             complement(155..1027)
                     /gene="rfbA"
                     /locus_tag="PK362_04375"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNC74105.1"
                     /translation="MKGIVLAGGSGTRLYPLTRGISKQLVPIYDKPMIYYPISVLMLA
                     GIREILIITTPHQQADFKRLLGDGRWLGLRLSYAVQPDPGGLAQAFTIGRDFVGDDRV
                     ALVLGDNIFFGHDFTGFLRRAARRKTGATVFAYPVRNPEAYGVVSFDSRGKARRIEEK
                     PRRPRSTHAVTGLYFYDNDVLDIAAKLRPSRRGELEITDVNNAYLRRGRLHVERLGRG
                     IAWLDTGTHESLLQAAVFIQTLQERQDLKVACLEEIAFDRGYISAEDLRRLAEPMRNN
                     EYGQYLLRLLKNRS"
     gene            complement(1044..2573)
                     /locus_tag="PK362_04380"
     CDS             complement(1044..2573)
                     /locus_tag="PK362_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNC74106.1"
                     /translation="MIRRFALTRRDRAALRRRGLTPADAARQRERLIRPAAPLRLDRP
                     CRVGDGIERWSPARRRALDRLARAGARRGLFSVLVPASGAASRLFQNLETLRRRRDAT
                     GRPVASLVREKAFSESRVFFKHLDRLALAGRLDAACRRRGASRQRLLTAGRWDEVLDA
                     LFESGTASLPKGLVPFHGRGPGARTAFEEHLRAAATIVGDDRGRCRMHFTVAPDQRRA
                     FAREARALQRKWGKRLGVRWRIQWSVQSPTTDTLALEKDGGWAREESGDLYFRPGGHG
                     ALLGNLEKTRAPFVFIRNIDNVSDAPTDPAAAAWRRALGGRLMDLWEEGRRWLRVLEA
                     GGPMEGAAAFVASTLGGRPPSKNPRSWLGRILRRPWRVCGMIAQRGDPGGGPFWVRAS
                     SRSEGGPSKQIVEESQVGAGQRRLWRRSTHFNPVDMVVALRDHRRRPYRLADFADDRS
                     VLVMEKMFRGRPVRVWERPGLWNGGMHRWNTVFVEIPAALFTPVKRVTDLLRPGHRRA
                     H"
     gene            complement(2617..2775)
                     /locus_tag="PK362_04385"
     CDS             complement(2617..2775)
                     /locus_tag="PK362_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74107.1"
                     /translation="MSDTLCPKCNAKGAVTVKQSRTDKGKDLKYYECGKCRSLWTNSI
                     DIAALPVE"
     gene            complement(2777..3079)
                     /locus_tag="PK362_04390"
     CDS             complement(2777..3079)
                     /locus_tag="PK362_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNC74108.1"
                     /translation="MGRLLRRRGFWIGLAAAGGVVAISGESVRGYLAQRREIRRLEGQ
                     LAEARRRATEQESRLVRSASDAYIETSARRELGLLRPGEIEFRFVAADDVEPAQEK"
     gene            complement(3082..>3210)
                     /locus_tag="PK362_04395"
     CDS             complement(3082..>3210)
                     /locus_tag="PK362_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HNC74109.1"
                     /translation="GQIKTGSASRSERIAKYNQLLRLEESLGAAARFPGAKAFLRG"
BASE COUNT          564 a         1055 c         1100 g          491 t
ORIGIN      
        1 gcggtaaatg tagggcgctc cgccggtggc gtcggctccc caacggattt ccccggtggc
       61 gcgggcccgt tcccaggcgt cggtcgcccg gagaccgacg ggcaggaggg cgagaataaa
      121 aagcgcggcg aggcgcacgg cggcgggacg acggtcagga acggtttttg aggagccgca
      181 gcagatactg gccgtattcg ttgttccgca tgggttccgc caggcggcgg agatcctccg
      241 cggaaatgta gccgcgatcg aaggcgattt cctcgagaca ggcgactttc aggtcctgac
      301 gctcctgcag ggtttgaatg aacaccgcgg cctgaagcag cgattcgtgg gtgccggtgt
      361 cgagccaggc gatgccgcgc cccaggcgct ccacgtggag gcgcccccgt cggagataag
      421 cgttgttgac gtccgtgatt tccaattccc cccgccggga cgggcggagc ttggcggcga
      481 tgtccaacac gtcgttgtcg taaaagtaga gcccggtgac ggcgtgggtc gaccggggcc
      541 ggcggggttt ttcctcgatg cgccgggctt tgccccggga atcgaaggag accaccccgt
      601 acgcttcggg gtttctcacc ggataggcga acaccgtggc ccccgttttt cgccgggccg
      661 cccgtcgaag aaagcccgta aagtcgtgcc cgaagaaaat gttgtcgccc aggaccagcg
      721 ccacgcggtc gtcgcccacg aaatcgcgcc cgatggtgaa ggcctgggcc aaaccgcccg
      781 ggtccggctg gacggcgtac gacagtcgga gccccaacca gcggccgtcc cccaacaatc
      841 gcttaaaatc ggcctgctgg tggggcgtcg tgatgatcag gatctcccga atcccggcca
      901 acatcagaac cgagatcgga taataaatca tgggtttatc gtagatcggc accagctgtt
      961 tgctgatgcc gcgggtgagc ggatagagac gcgtgccgga accgccggcc agaacgatgc
     1021 ccttcatggg aggaaataat acatcaatgg gcgcgccggt gtcccgggcg caggagatcg
     1081 gtcacccgct tgaccggcgt gaagagagcg gccggaattt cgacgaacac cgtgttccaa
     1141 cggtgcatgc ccccgttcca aaggccgggt cgttcccaca cgcgtacggg gcggcctcga
     1201 aacatttttt ccatcacgag gaccgaccgg tcgtcggcga aatcggccag ccggtagggg
     1261 cgtcggcgat ggtcccgcag ggcgaccacc atgtccacgg ggttgaaatg ggtcgaacgg
     1321 cgccacagac gtcgctgacc ggcccccacc tgggattcct ccacgatttg cttgctcggg
     1381 ccgccctccg accgggagga agcccggacc caaaaaggcc ctccgcccgg gtccccccgc
     1441 tgagcgatca tgccgcacac gcgccaggga cgccgcagaa tccggcccag ccaggaacgc
     1501 gggtttttcg acgggggacg cccccccagg gtcgacgcca cgaaggcggc ggcgccctcc
     1561 atcgggcccc cggcctccag gacccgaagc caccggcggc cttcttccca caaatccatc
     1621 agtcgcccgc ccagggcccg gcgccaggcc gcggcggcgg ggtccgtcgg ggcgtccgac
     1681 acgttgtcga tgttccgaat aaaaacgaag ggggcccggg tcttttccag gttgcccaga
     1741 agggcgccgt ggccgcccgg ccgaaaatag aggtcgcccg actcctcccg ggcccagccg
     1801 ccgtcttttt ccaaggccag ggtgtcggtg gtcggcgatt gaaccgacca ctggatccgc
     1861 catcgaacgc cgagccgctt tccccatttc cgttgaaggg cccgggcctc ccgggcgaag
     1921 gcccgccgtt ggtccggggc cacggtgaaa tgcatccggc accgcccccg gtcgtccccg
     1981 acgatcgtgg cggccgcccg aagatgttcc tcgaaggccg tccgggcccc cggtccccgg
     2041 ccgtgaaagg gcaccagacc cttgggcagg gacgccgtgc ccgattcaaa gagggcgtcc
     2101 aggacctcgt cccaacggcc ggcggtcaac aaacgttgcc gggaagcccc ccggcgacgg
     2161 caggccgcgt ccagacggcc cgccagggcc aaacggtcca ggtgtttgaa aaaaacccgg
     2221 gattcggaaa aagctttttc gcgaacaagg gaggcgacgg gccgtcccgt ggcgtcccgg
     2281 cggcggcgaa gggtttccaa attctgaaaa aggcggctgg cggcgccgga ggcgggaacc
     2341 aaaacggaaa aaagtccgcg acgggccccg gcccgggcga gacgatccaa cgcgcggcgg
     2401 cgggcggggg accaccgttc aatgccgtcg cccacgcggc agggacggtc caaacgaagg
     2461 ggggcggcgg gccggatcag gcgttcgcgc tggcgggcgg cgtcggcggg ggtgaggccc
     2521 cggcggcgga gggccgctcg gtcgcggcgc gtcagggcga atcggcggat caaagggacg
     2581 gacggaatcc gggagccgac ggcgccggga ccggcgtcat tccaccggaa gggcggcgat
     2641 gtcgatggag ttggtccaaa ggctgcggca ttttccgcat tcgtagtact tgaggtcttt
     2701 gcctttgtcc gttcgggatt gcttgaccgt cacggcgcct ttggcgttgc atttggggca
     2761 gagggtatcg gacatgttat ttctcctggg cgggttcgac gtcgtccgcc gccacaaaac
     2821 ggaattcaat ttccccgggg cgcaaaagcc ccaattcccg acgggccgat gtttcgatgt
     2881 aggcgtcgct ggcggagcgg accagtcgcg attcctgttc cgtggcccgg cgccgcgcct
     2941 cggccaattg accttccaac cggcggattt cccgccgctg ggccagataa ccgcgcacgc
     3001 tttccccgct gatcgccacg actcccccgg cggccgcgag gccgatccaa aaaccccggc
     3061 gccgaagaag acggcccacg tttacccgcg caggaaggcc ttcgcgcccg gaaaccgggc
     3121 cgccgcgccc agagattcct cgaggcgaag caattggttg tatttggcga tccgctcgga
     3181 gcgggaggcg gaccccgttt tgatttggcc
//
LOCUS       DAOQLS010000178         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16474_length_3201_cov_4.602670, whole genome shotgun sequence.
ACCESSION   DAOQLS010000178 DAOQLS010000000
VERSION     DAOQLS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16474_length_3201_cov_4.602670"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK362_04400"
     CDS             complement(<1..506)
                     /locus_tag="PK362_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNC74110.1"
                     /translation="MTAFWLAIGTGAARAASPVDDLDRVLRTRVHIDGRVDYAGLRGA
                     DRAGLDRYLAWAATASVAGWSDARRSAFWINTYNARVLAVVADRPTLRSISDDFALFD
                     APFDAAAQRLSLNDIEHRILRGKSRQGQKPLPALGPAGFDPRIHFALVCGARDCPRLR
                     SFAYTEDNL"
     gene            745..2130
                     /locus_tag="PK362_04405"
     CDS             745..2130
                     /locus_tag="PK362_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNC74111.1"
                     /translation="MDPLSPQSQLHLFAQPERNKITHIIKRDGRLVAFNKLKIVNAIY
                     RAAVAVGGRDRDRSEALANQAMKALGAVYPAGSTPSVEEIQDVVEKVLIENGHAKTAK
                     AFILYRLERARTREKKPEVTAVEDYIPYKVLWRVLSWNVDHGCHTVEGLNAQMAGGWK
                     KLIRDAERAYHDEIHHVADIVLKRLPDVRLVIVAGPSSSGKTTTTMKISERLKSKGHP
                     FLLLNLDNYFRELSEQPKDEYGDYDFEMPVALDLPLINEHLGQLMEGRPIRMPIYNFK
                     TGRREKETKEFRLRENEILLIDSLHGLYDGMTGGVAPETKFKFYIEALSQIKDADGEY
                     VRWADLRLMRRMVRDSWHRSYDPAMTVGHWHYVRRSELRYIVPYIQRVDYIFNGALPY
                     ELPYHRARLADHMPAIVEKFKGDPKKADALLRAERLQRLMAALKSPASTDDVPDDSLL
                     REFIGGSRYHY"
     gene            2151..2447
                     /locus_tag="PK362_04410"
     CDS             2151..2447
                     /locus_tag="PK362_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNC74112.1"
                     /translation="MSPTSTGARAAADRPETTDRGRRGLGWKTILFNCDCHSFQEVAL
                     QLMKAIRCDYTRGLALANVVHHTGSATVYSGPRERCEAVAAVLEDIRLRVQVAQ"
     gene            2491..2835
                     /locus_tag="PK362_04415"
     CDS             2491..2835
                     /locus_tag="PK362_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74113.1"
                     /translation="MVRKKKATPARSSEPAGAGPSITDEAAKAYVAVDYPQEGEIVTS
                     ASYTIRLTTSAHAGVELSIDGKEFQPARESVGHWWLDWSGYGSGPHAIVARIAVGKRA
                     LKSKARSFTVLI"
     gene            2896..>3201
                     /locus_tag="PK362_04420"
     CDS             2896..>3201
                     /locus_tag="PK362_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74114.1"
                     /translation="MTLPVRGRPAPRGSSCALPADFWAEAARWIAHNTVTSRSNLPFL
                     RRLRSLFAALGFRTRLDLYREGTASFGNLILSRGPRRGRPLLLNTHSDTVPPGPAARW
                     "
BASE COUNT          615 a         1040 c         1027 g          519 t
ORIGIN      
        1 agattgtctt cggtgtaggc gaaggacctt agccgggggc agtcccgggc gccgcacacg
       61 agggcgaaat gaatccgagg gtcgaacccg gcggggccga gcgcgggaag cggtttttgg
      121 ccttggcggg actttccgcg gagaattcgg tgttcaatgt cgttcaacga cagccgctgg
      181 gcggccgcgt cgaagggagc gtcgaaaagg gcgaaatcgt cgctgatgga ccgaagcgtc
      241 ggccggtcgg ccacgacggc caacacccgc gcgttgtagg tgttgatcca aaaagccgag
      301 cgccgggcgt cgctccatcc ggcgacggac gccgtggcgg cccaggccaa ataacggtcc
      361 aggcccgccc ggtcggctcc gcgcagcccg gcgtaatcca cccggccgtc gatgtggacc
      421 cgggtccgca ggactcggtc gaggtcgtcg acgggggaag ccgcccgggc ggcgcccgtt
      481 ccgatggcca gccagaaagc agtcacgacg gttttcgcgt tcagggggac acctcccaaa
      541 agggcacgat gccttttcgg cgaggctccc gcaagcgggg cgcgcttccg gtccttgacg
      601 cggaggggtc gcgggaataa accgccctcc cgtattcgca cgggacagag cccggtgcga
      661 atggcgggcg agcttggatg ggcgaggcca tcccttgacg gcgggtcccc aaatggggca
      721 aaatacccct gagatcgacg gaaaatggac cccctctcgc cccaatccca gttgcacctg
      781 ttcgctcaac ccgagcgcaa caagatcacg cacatcatca aacgcgacgg ccgcctggtg
      841 gccttcaata aattaaaaat cgtgaacgcc atctaccgcg cggccgtcgc cgtcggagga
      901 cgggaccgcg accgctccga ggccctggcc aaccaggcga tgaaggccct gggcgccgtt
      961 tatccggcgg ggtcgacccc ttcggtcgag gaaatccagg acgtcgtgga gaaagtgctg
     1021 atcgaaaacg gccacgcgaa aacggccaag gcgttcatcc tctaccgcct ggaacgggcc
     1081 cgaacccggg agaaaaaacc ggaggtgacc gccgtcgagg attacatccc ctacaaggtg
     1141 ctctggcgcg tgttgtcctg gaacgtggac cacgggtgcc acacggtcga ggggttgaac
     1201 gctcaaatgg cgggcggttg gaaaaaactc atccgggacg ccgaacgggc ctaccacgac
     1261 gaaatccacc acgtggccga catcgtgttg aagcgcctgc ccgacgttcg cctggtcatc
     1321 gtggccgggc cctcgagctc cggcaaaacg acgacgacga tgaaaatcag cgaacgcctc
     1381 aaatccaagg gccacccctt ccttcttctg aacctggaca attatttccg ggagctttcc
     1441 gagcagccga aggacgaata cggcgactac gatttcgaaa tgccggtggc cctggacctt
     1501 cccctgatca acgagcacct cggccaattg atggaggggc ggccgatccg catgccgatt
     1561 tacaatttca aaaccggccg acgggaaaag gaaacgaagg agttccgctt gcgggaaaac
     1621 gaaatccttc tgatcgacag cctgcacggg ctgtacgacg gcatgaccgg cggcgtggcg
     1681 cccgaaacga agttcaagtt ttacatcgaa gctttaagcc agatcaagga cgcggacggg
     1741 gaatatgtcc gctgggccga tttgcgcctc atgcggcgca tggtccggga cagctggcac
     1801 cgaagctacg acccggcgat gacggtcggc cattggcatt acgtgcggcg cagcgaactg
     1861 cgctacatcg tgccgtacat ccagcgggtc gactacattt tcaacggggc ccttccctac
     1921 gaactgccct accaccgggc gcgcctggcg gaccacatgc ccgccatcgt cgagaagttc
     1981 aaaggggatc cgaaaaaggc cgacgccctc ctgcgggccg aacggctcca gcggctcatg
     2041 gcggctttga aatcccccgc ctccaccgac gacgtgccgg acgattccct gctgcgggaa
     2101 ttcatcggcg gcagccggta ccattattag cgaaatgcta aaatgagggc atgagcccga
     2161 cgtcgaccgg ggcccgggcc gccgcggacc ggcccgaaac caccgatcgc ggacgccgtg
     2221 ggctggggtg gaaaacgatt ttgttcaatt gcgattgcca ctcgtttcag gaagtggctc
     2281 tgcagttgat gaaggccatc cgttgcgact acacgcgcgg gttggccctg gccaacgtgg
     2341 tgcaccacac cggcagtgcg accgtctatt ccggcccgcg ggaacggtgc gaagcggtgg
     2401 ccgccgtgtt ggaagacatc cgattgcggg tgcaggtcgc ccagtgacat cgaggaatcc
     2461 cgggaggttt taatgttcgg gaggaaaacc atggtacgca agaagaaggc aacgccggcg
     2521 cgttcgtccg aacccgccgg ggcggggccg tcgatcacgg acgaggccgc gaaggcctac
     2581 gtcgccgtcg actatcccca ggaaggcgag atcgtgacca gcgcgtcgta cacgatccgt
     2641 ctgacgacga gcgcccacgc gggggtcgag ttgtccatcg acgggaaaga gttccagccc
     2701 gcccgcgaga gcgtggggca ttggtggctc gactggtccg gttacggatc cggcccccac
     2761 gccatcgtcg cccgcatcgc cgtggggaaa cgcgctttga aatccaaagc gcggtccttc
     2821 acggtcctta tttaaccggc ccgcttcgtt cgtgtcgaag ccccggcggt cgcggttcgg
     2881 gcgacgcccc gcgccgtgac cctccctgtc cgggggcgcc ccgccccccg cggctcgtcc
     2941 tgcgccctgc ccgcggattt ttgggccgaa gccgcccgct ggatcgccca taacaccgtc
     3001 acctcccgct ccaacctccc ttttctccga cgtctccgga gtctttttgc cgccctgggg
     3061 ttccgcacgc gattggacct gtatcgggag gggacggcct cctttgggaa tttgattttg
     3121 tcccggggcc cccgccgggg ccgcccgctc ctcctgaaca cccactccga cacggtcccc
     3181 cccggtcccg ccgcaaggtg g
//
LOCUS       DAOQLS010000179         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16584_length_3186_cov_4.450655, whole genome shotgun sequence.
ACCESSION   DAOQLS010000179 DAOQLS010000000
VERSION     DAOQLS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16584_length_3186_cov_4.450655"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="PK362_04425"
     CDS             <1..1583
                     /locus_tag="PK362_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNC74115.1"
                     /translation="PAHTQHDGVDFVPAKGPMLLAQHFSAIAAAGPIVGPILAGLWFG
                     WLPALLWIVLGSIFLGAVHDYASLVGSVRHRAQSVAELFREHIGPRAYKFFVAFVWLS
                     LIYVITAFTDITSASFMEAQYGGGVATSSLVYLLIGLGLGVLLTKTRTPLWAATLVAV
                     PLVALSIWYGPSFPLKIPAGGMIRPQLAWDYIILAYCFFASMIPLWLLLQPRGYLGGF
                     FLYGTLIAGVAGLFLGGDRAQYPAFIGFTSKGGMPLFPMLFVTVACGACSGFHGLVGS
                     GTTSKQIDVETDCRLVGYGGMLLEALVAVVALATLMLLPAGDASLSLGPDRIYANGLA
                     RFVERFGLNPDLARSFALLAFATFIYDTLDVTTRLGRYMLQELTGWKGVKGGAAATLL
                     TLALPAYFVTLQMTDAAGAPIPAWKLFWTIFGTSNQLLAGLTLLGLTLWLKRTGRFWL
                     VTAVPMAFMMGMTLWALGRTIGPWAATLFSNPHWETIPIVAVVLSGLAILLIVESLGT
                     LRRALPESEPELQLERAE"
     gene            1584..2033
                     /locus_tag="PK362_04430"
     CDS             1584..2033
                     /locus_tag="PK362_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC74116.1"
                     /translation="MQNFDLLHTLLLGWPILSVLLVFSIVTVAVLFESWTTLKWVRVF
                     LAKPDGEKDAKPLIEKIEQRLAMLGTIANAAPFVGLLGTVIGIIRAFHTISQAAGSGG
                     GMTLVAGGISEALVSTAAGLAVAIPASMIYNYFTFHLERLQRLSRGA"
     gene            2033..2485
                     /locus_tag="PK362_04435"
     CDS             2033..2485
                     /locus_tag="PK362_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC74117.1"
                     /translation="MDIARRKRMITEINMVPFIDIVLVLLIIFMILSPFLAQSQIPVN
                     LPRAATTRPGDDENPLRLRITRDGDYYLGDRRILRDDLPAKLAETAQPGRAVLIEADR
                     DVSFKNVVLALDAAQKLKAVKVGVAVLTATPDDKKPEADTAVAAPGNP"
     gene            2482..>3186
                     /locus_tag="PK362_04440"
     CDS             2482..>3186
                     /locus_tag="PK362_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNC74118.1"
                     /translation="MNRYLTYSSLIHATALAVVAYLNFQAEKPQIYYGFQFLGGQSGF
                     GTGKLEPAPAPLANPPAAAGKPAETPVAPPEPSADPDRIALPKEADKPTVKKPAAEVE
                     PQGKNTKKGKGTKDGRGEASFGHGDFQGAKTGPVGGIGTSLEIGGFGPGSAGLPVSPF
                     PFKWYGELVYKRLWESWDRTDAGTRECKVAFLIQKDGQVRDIRVKNSSGDSYFDMTAK
                     RAVTAAAPFPPLPDGFE"
BASE COUNT          523 a         1065 c          961 g          637 t
ORIGIN      
        1 cccccgccca cacacaacac gatggcgtcg attttgtccc cgccaagggc cccatgctcc
       61 tggctcagca cttctcggcc atcgcggcgg cgggtcccat cgtggggccc attctggccg
      121 gcctttggtt cggttggctg ccggccctgc tctggatcgt cctgggatcc atcttcctcg
      181 gcgccgtgca cgattacgcg agcctcgtgg ggtcggtccg ccaccgggcc caaagcgtgg
      241 cggagctgtt ccgggaacac atcggccccc gggcctataa attcttcgtg gccttcgtgt
      301 ggctctcctt gatttacgtg atcacggcct tcaccgacat caccagcgcc agcttcatgg
      361 aagcccaata cggcggcggg gtagcgacct cctccctcgt ctatctattg atcggtcttg
      421 gccttggcgt tctactgacc aaaacccgca cccctctctg ggccgccacc ttggtggcgg
      481 ttcccttggt ggccctgtcc atctggtacg ggccgtcctt tcccctcaaa atacccgccg
      541 gggggatgat ccggccccaa ttggcctggg attacatcat cctggcctac tgctttttcg
      601 cttccatgat ccccctgtgg cttttgcttc aaccccgggg ctatctgggc gggtttttcc
      661 tctacggcac tttaatcgcc ggggtggcgg gcctgttcct gggcggggat cgggcccagt
      721 atcccgcttt tatcggcttt acctccaagg gcggcatgcc gctgtttccc atgctcttcg
      781 ttacggtggc ctgcggggcc tgctccggct ttcacgggct cgtcggttcc ggcacgacct
      841 ccaaacaaat cgacgtggaa accgattgcc ggttggtggg atacggcggc atgctcctgg
      901 aagccctggt ggcggtggtc gccctcgcca ccctcatgct tctgccggcc ggagacgcct
      961 cgctctcgct gggcccggac cgaatttacg ccaacggcct ggcgcgattt gtggagcgct
     1021 tcggtttgaa ccccgacttg gcccgaagtt tcgccctcct ggccttcgcc acgttcattt
     1081 acgacaccct ggacgtcacc acgcgactgg gccgctacat gcttcaggaa ctaacgggtt
     1141 ggaaaggggt caaaggaggg gcggccgcca cgctgttgac cttggcgctc ccggcctatt
     1201 tcgtgacctt gcaaatgacc gacgcggcgg gcgcgccgat cccggcgtgg aaactttttt
     1261 ggacgatctt tggaacgtcg aaccagctcc tggccggttt gacgctcctg ggcctcaccc
     1321 tgtggctgaa acgcacgggg cggttttggc tcgtgaccgc ggtgcccatg gcgtttatga
     1381 tgggcatgac gctgtgggcc ctgggccgca ccatcgggcc ctgggcggcc acgttgtttt
     1441 caaatcccca ctgggaaacc atcccgatcg tcgccgtcgt gttgtccggc ctggcgatcc
     1501 tgctgatcgt ggaatccctg ggcacgctcc gccgggccct cccggaatcg gagcctgaac
     1561 ttcaattgga aagggcggag taaatgcaaa actttgatct gcttcacacc ctgttgctgg
     1621 ggtggccgat tctgagcgtg ctgctggtct tctccatcgt gaccgtggcc gtgttgttcg
     1681 agtcctggac caccctgaaa tgggtgcggg tgtttctggc caaacccgac ggcgaaaaag
     1741 acgccaaacc cttgatcgaa aagatcgaac aacgcctggc catgctgggc accatcgcca
     1801 acgccgcgcc cttcgtgggc ctcctgggca ccgtgatcgg catcatccgg gcctttcaca
     1861 cgatctcgca ggcggcgggc tccggcgggg gcatgacctt ggtggcgggc ggcatttcgg
     1921 aggctctggt ttcgacggcg gcggggttgg ccgtggccat tccggccagc atgatctaca
     1981 actacttcac gttccacctc gagcggttgc agcggctttc ccggggcgcc tgatggacat
     2041 cgcccgtcgc aagcggatga tcacggaaat caacatggtg ccgttcatcg acatcgtgtt
     2101 ggtcctcctg atcatcttca tgatcctctc gccctttttg gcccaaagcc aaattccggt
     2161 gaacctgccc cgggccgcca ccacccggcc gggggacgac gagaaccccc tgcgccttcg
     2221 catcacccgg gacggcgact attatctggg cgaccgacgg atccttcggg acgacctgcc
     2281 ggccaagctg gccgaaaccg cccagccggg acgggcggtc ttgattgaag ccgaccggga
     2341 cgtgtctttt aagaacgtgg tcctggccct ggacgcggcc caaaaactca aggccgtcaa
     2401 ggtgggcgtg gccgtattga ccgcgacgcc cgatgacaaa aaaccggagg cggacacagc
     2461 cgtggccgct cccggaaatc cgtgaaccgc tacctgacct actcctccct gatccacgcg
     2521 acggccctgg cggtcgtcgc ctatcttaat ttccaggcgg aaaagcccca aatctattac
     2581 ggttttcagt ttttgggcgg ccagtcgggt ttcggcacgg gaaaattgga gccggccccc
     2641 gcccccctgg ccaatccccc ggccgccgcg gggaaacccg ccgaaacccc cgtcgcgccg
     2701 ccggaacctt ccgccgaccc ggaccgcatc gctctgccca aggaggcgga taaaccgacc
     2761 gtcaagaagc cggcggccga agtcgaaccc caggggaaaa acaccaaaaa agggaaagga
     2821 accaaggacg gccggggcga agcctctttc ggccacggcg attttcaagg ggccaaaacc
     2881 ggccccgtgg gcggcatcgg cacctccctg gagatcggcg ggttcgggcc cggaagcgcc
     2941 ggcttgccgg tgtccccgtt tccgttcaag tggtacgggg agctggtcta caagcgcctc
     3001 tgggagtcct gggaccggac cgacgcaggc acccgggaat gcaaagtggc tttcctcatt
     3061 caaaaagacg ggcaagtccg ggacattcgg gtgaagaatt cctccgggga ctcctatttc
     3121 gacatgacgg ccaagagggc agtcacggcc gccgccccct tcccccccct ccccgacggt
     3181 ttcgag
//
LOCUS       DAOQLS010000180         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16604_length_3184_cov_3.976670, whole genome shotgun sequence.
ACCESSION   DAOQLS010000180 DAOQLS010000000
VERSION     DAOQLS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16604_length_3184_cov_3.976670"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /gene="ligA"
                     /locus_tag="PK362_04445"
     CDS             <1..1200
                     /gene="ligA"
                     /locus_tag="PK362_04445"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNC74119.1"
                     /translation="TCARFRENRADLPFEADGAVVKVDRIPFQRRLGMTFKSPRWAVA
                     YKFPAAQATTRVRDVEFSVGRTGAVTPVAKLEPVECGGVTISNASLHNFDEIERLGLR
                     IGDWVLIERAGEVIPKVLSVIVGRRTGEEKAIPIPKACPACGGPVVKTKEGEVIFRCA
                     NPACPAQVEKNLLHFASRDAMDIQGMGEAVVQALLARDRVKDLADIYALTKDQLLELE
                     GFKDKKADNLLAAIEKSRRGSLGRFLNGLGIRDVGEKGALTLARHFRTLDRLRAATAE
                     DLQALPDVGPVMAESVTSFFAQTSVRRLLDKFKRLGLEPGEEAPVAGGPLAGKTVVFT
                     GTLTKCTRAEGEGIVRSQGGKATGSVSTATSFVVAGAEAGSKLRQAQKLGVEVIDEAE
                     FRRRAGL"
     gene            1243..1731
                     /locus_tag="PK362_04450"
     CDS             1243..1731
                     /locus_tag="PK362_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNC74120.1"
                     /translation="MTADDDFRHAMDLALRLLKFRARTRAELADRLKRKKVEAALVPR
                     VVDRLTELKLVDDAELARQTVESRRRANQGDHRIRRDLRKRGLGSDAVEDALAAAETP
                     AGSDADRAWEALRRRAGRMKALDPRVARRRLEGYLFRQGFDADDVRSALRRFFSNQGE
                     DE"
     gene            1728..>3184
                     /gene="purH"
                     /locus_tag="PK362_04455"
     CDS             1728..>3184
                     /gene="purH"
                     /locus_tag="PK362_04455"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNC74121.1"
                     /translation="MKTVEIRRALISVSSKNQIVELAHGLSNLGVEILSTGGTAKSLR
                     QAEIKVREVSDLTGFPEILDGRVKTLHPKVFGGLLALRDKAAHSQDMEKHGIPPIDLV
                     VVNLYPFETVCNDTNIPQDEMLEYIDIGGSALIRAAAKNYSHVAPLCDPDDYALVLEE
                     LNERGKLSPETRQRLAAKAFSHTAHYDAVIANYFRHKTQSKEFPAEIAAGLRKKQDLR
                     YGENPHQKAALYMESGNKAWGVVGAKVLQGKQVSYNNYLDCDAAWRLVCSFANPASVV
                     IKHNNPCGVSEAEVQVDAFRRALAADSVSAFGGIVGFNRAVDADTAQELSKLFLECVI
                     APGYHPEAREILGKKANLRLLEQSTLLADPYEWDIRKISGGFLMQEQDLPRPVETKSV
                     TRRAPSPEEQMSLAFAWQVVKQVKSNAIVLVRGRTTCGIGAGQMSRIDSMKVAHMKMA
                     APGIDVKKPLPLVMASDGFFPFRDTVDEAAKMGVAA"
BASE COUNT          611 a         1039 c         1019 g          515 t
ORIGIN      
        1 acctgcgccc gctttcggga aaaccgggcc gacctgccct tcgaggccga cggggccgtg
       61 gtcaaggtgg accgcatccc ctttcaacgc cgcctcggga tgacctttaa atcgccccgg
      121 tgggcggtgg cctacaaatt tcccgcggcc caggccacga cccgggtgcg ggacgtggag
      181 ttttccgtgg gccggacggg ggccgttacg cccgtggcca aactggagcc cgtggaatgc
      241 ggcggcgtga ccatctccaa cgcctccctc cacaatttcg acgagatcga gcgcctgggc
      301 cttcggatcg gcgactgggt tttgatcgaa cgggccgggg aggtgatccc caaggtgttg
      361 tcggtgattg tgggccgccg gacgggggaa gaaaaagcca tcccgattcc caaggcctgc
      421 ccggcctgcg ggggacccgt cgtcaagacc aaggaggggg aagtgatttt ccgttgcgcg
      481 aacccggcct gcccggccca ggtggagaaa aacctcctcc acttcgcgag ccgggacgcc
      541 atggacatac agggaatggg ggaagcggtg gtccaggcgc ttttggcccg ggaccgggtg
      601 aaggacttgg ccgacatcta cgccctgacc aaggaccaat tgttggaact ggagggattc
      661 aaagacaaaa aggccgacaa tctgttggcg gccattgaaa aatcccggcg cgggtccctg
      721 gggcggtttt taaacgggtt gggcattcgg gacgtgggcg aaaagggcgc tctgaccctc
      781 gcccgccatt ttcgaaccct ggaccgcctc cgcgccgcca cggcggagga tctgcaggcc
      841 ctgccggacg tggggcccgt catggcggag tccgtgactt cttttttcgc ccagacttcc
      901 gtccggcggc ttctggacaa attcaagcgc ctgggcctgg agcccggaga ggaagcgccg
      961 gtggccggcg gccccctggc ggggaaaacc gtggtcttta ccggaaccct tacaaagtgc
     1021 acccgggccg agggcgaagg gatcgtacgg tcccaggggg ggaaggccac ggggtccgtc
     1081 tccaccgcga cctccttcgt cgtggcgggg gccgaagccg gttccaagct tcgccaagcc
     1141 caaaagctcg gggtcgaggt gatcgacgaa gccgagtttc gccgccgggc cggactttga
     1201 cgaatttgtt aaaatgaacc cgttccgatc cccccatctc ccttgacggc cgacgacgac
     1261 tttcgccacg ccatggattt ggccctccgg ttattgaagt tccgggcccg gacccgggcg
     1321 gaattggccg accgcctaaa acgcaaaaaa gtggaggcgg ccctggtccc ccgcgtggtg
     1381 gaccgcctga ccgaattgaa attggtggac gacgcggagc tggcccgtca aaccgtcgaa
     1441 agccgccgcc gggcgaacca gggcgatcac cgcatccgac gggatttacg caagcggggg
     1501 ctcgggtccg acgcggtgga ggacgctctg gccgcggcgg aaacgcccgc cggatccgac
     1561 gcggaccggg cctgggaggc cctgcggcgc cgggccggcc ggatgaaagc cctcgacccg
     1621 cgggtcgcgc ggcggcgcct ggaaggctac cttttccgtc aggggttcga cgcggacgac
     1681 gtccgctcgg ccctccggcg atttttttcg aaccaaggag aagacgaatg aaaaccgtcg
     1741 aaattcggcg agcgcttatt tccgtatcga gcaaaaacca gatcgtggaa ctggcccacg
     1801 ggttgtccaa cctgggggtg gaaatcctgt ccacgggggg gacggccaag tccctccgcc
     1861 aggccgaaat caaggtccgg gaggtctcgg acctcacggg ctttcccgaa attctggacg
     1921 gccgcgtcaa aaccctccat ccgaaggtct tcggcgggtt gttggccctg cgggacaagg
     1981 ccgcccacag ccaggacatg gaaaagcacg gcattccgcc catcgacctg gtggtcgtca
     2041 atctttaccc ctttgaaacc gtctgcaacg acacgaacat tccccaggac gagatgctcg
     2101 aatacatcga catcggcggg tcggccctga tccgcgccgc ggccaagaac tacagccacg
     2161 tggcgcccct ctgcgatccg gacgattacg cgctcgtgct ggaggagttg aacgaacggg
     2221 gcaaactctc cccggaaacg cgccagcgcc tggcggccaa ggccttcagc cacacggccc
     2281 attacgacgc cgtcatcgcc aactacttcc gccacaaaac ccagtccaag gaatttccgg
     2341 ccgaaatcgc cgccggtctt cggaaaaaac aggacctccg ctacggcgaa aacccccacc
     2401 agaaagccgc cctctacatg gaatcgggca acaaagcctg gggcgtcgtg ggagccaagg
     2461 tgctccaggg aaagcaggtc tcctacaaca actatttgga ctgcgacgcc gcctggcggc
     2521 tggtctgctc cttcgccaac cccgccagcg tcgtcatcaa gcacaacaac ccctgcggcg
     2581 tgtccgaagc cgaggtccag gtcgacgcct tccgtcgggc cctggcggcc gattccgtgt
     2641 ccgccttcgg cggcatcgtg ggcttcaacc gcgccgtgga cgcggacacc gcccaggaac
     2701 tctcaaaact ctttttggaa tgcgtgatcg ccccggggta tcacccggaa gcccgggaaa
     2761 tcctcgggaa aaaggcgaac ttgcgcctcc tggagcaatc cacgcttttg gcggacccct
     2821 acgaatggga catccgcaaa atctccggcg ggtttctcat gcaggaacag gacctccccc
     2881 ggccggtgga aaccaagtcg gtgacccgcc gggcgccctc gccggaggaa caaatgtccc
     2941 tggcgttcgc ttggcaggtc gtcaaacagg tcaagtccaa cgccatcgtg ttggtgcggg
     3001 gccgcacgac ctgcggcatc ggcgccggcc agatgagccg catcgattcc atgaaggtgg
     3061 cccacatgaa aatggcggcc cccggcatcg acgtcaaaaa gcccctcccc ctggtcatgg
     3121 cctccgacgg tttcttccct ttccgggaca ccgtggacga ggcggccaag atgggcgtgg
     3181 cggc
//
LOCUS       DAOQLS010000181         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16644_length_3178_cov_3.919629, whole genome shotgun sequence.
ACCESSION   DAOQLS010000181 DAOQLS010000000
VERSION     DAOQLS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16644_length_3178_cov_3.919629"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..868)
                     /locus_tag="PK362_04460"
     CDS             complement(173..868)
                     /locus_tag="PK362_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC74122.1"
                     /translation="MDAVVVAEPLIEVTEVSKTYLVGTEKVHALQGVTLRVNEGEFLA
                     LAGPSGSGKTTLLNLLGLVESPSAGRIRLDDTVVTDVSPRRLTRLRAERLGYVFQTFN
                     LLPVLTVAENIEYPLFLRRVGRRDRWRRVNEMAHRVGLGSFMDHKPSQLSGGQRQRVA
                     IARALVGSPRVVLADEPTANLDTHTGEKILTLMKELNRETKATFIFSTHDPQIMAAAD
                     RVVRLHDGRIAAP"
     gene            998..2038
                     /locus_tag="PK362_04465"
     CDS             998..2038
                     /locus_tag="PK362_04465"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNC74123.1"
                     /translation="MKLLSVAHAVPGRRVDNDQEIAAFCRRNWMWWPLHRLFRKKLQQ
                     GFKLSGTRYRYRRAPGETASAIACRAAREALDKAGLAPGDIDMLIYVGVGRGVLEPAT
                     ANLFKSLLGLTNATCFDVLDACASWVRGMSIAQSFINQGTHKTVMVMNAELNYEEYCG
                     DHALRRPKDLYFRFAAHTIGEAATATIVRDDGRRDNFYFSMKNWENTYDLCQIPLPNR
                     DDFAGNYPPRTPQQNMVFVAASRELLTAAITKLTEQFKEDPRLNGQIYDLHFCHDVSD
                     YSTKLVAKALNVPLSMVYRTHERFGNTVSASIPLALSLALAEGRLKKGMNLLLTCGSA
                     GVTTAYLQFRYD"
     gene            2051..2461
                     /locus_tag="PK362_04470"
     CDS             2051..2461
                     /locus_tag="PK362_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74124.1"
                     /translation="MIFRARVAQRGWTRILAAGVLMYAYAAAFVVVDLLARFGQATVL
                     RLCGIPPVDRRRYIALDRGRVPPLTPFDGFNCNYCGYANGVLAWAREIVRRVEQYWCP
                     VRHAAPLDGDVDPATYAPAGDRAALDRWLEPPRP"
     gene            2458..>3178
                     /locus_tag="PK362_04475"
     CDS             2458..>3178
                     /locus_tag="PK362_04475"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC74125.1"
                     /translation="MTGRPPSVSKDLYPFASHYLERNGHRLHYVDEGRGAPVLLLHGN
                     PTWSFYYRRVIEDLRVDHRVIALDHLGCGLSDKPQDYPYRLENHIANVEYLVDALGLK
                     NLSLVLHDWGGVIGAGYAVRHPANVVRWALMNTAAFWPAGYLPLRLRLCRIPRVGEFL
                     IRRFNLFARIALRVAVRRPGGLTPEVRRAYLAPYDSYAHRIATARFVLDVPDSPRHPS
                     YATAQAVESGLAQLVRLPTRIF"
BASE COUNT          548 a         1057 c          972 g          601 t
ORIGIN      
        1 aggtggccga actgggaccg gatgtaactt tcccgaagct ggaccaggga atcgtcgatg
       61 aaaccgcccg cgaaaatgag ggccacggag ccgaaggcga tggcggccag ggtcaccagg
      121 gtccgcccgg gtcggcgaaa aatgttgcgg agggggagcg tccagtagaa cattagggcg
      181 ccgcgatccg gccgtcgtgc aaacggacca cccgatcggc cgcggccatg atttgcggat
      241 cgtgggtgga aaatataaag gtggccttgg tttcccggtt caattccttc atgagcgtta
      301 aaattttttc cccggtgtgg gtgtccaggt tggcggtggg ctcatcggcc aaaaccaccc
      361 gcggggaacc caccagggct cgggcgatgg ccacgcgttg gcgctgcccg ccgctcaatt
      421 gggacggctt gtgatccata aacgatccga ggccgacccg atgggccatt tcgttgaccc
      481 gccgccagcg gtcccgccgt cccacgcgcc ggagaaagag ggggtattcg atgttttcgg
      541 ccaccgtgag aaccggcaac aggttgaacg tttgaaaaac gtagcccagg cgttcggccc
      601 gcaggcgcgt caggcgccgg ggcgaaacgt cggtgaccac cgtgtcgtcc aaacggatgc
      661 ggccggcgga gggcgattcc accagcccca gcagattgag cagggtggtt ttgccgctgc
      721 cggagggccc ggccagcgcc aggaattcgc cttcgttgac ccgtagggtc accccttgaa
      781 gggcgtggac cttttcggtt ccgaccaggt aggttttgga aacctcggtg acttcgatca
      841 gcggttcggc gacaacgacg gcgtccatgg ttcctcttga caaaaaaggt ttggcatgtc
      901 aaaacagtat agggcacaat gatcaacact tcaagggcgc tcgcgtccgt tttccgtcgg
      961 tcgcggcgct ctttcttctt cgggagatct taaaatcatg aaactgctga gcgtggcgca
     1021 cgcggttccc ggtcggcgcg tcgataacga ccaagagatc gcggcgtttt gccgccggaa
     1081 ttggatgtgg tggccccttc accggctctt ccggaaaaaa cttcaacaag gttttaaact
     1141 ttcgggcacg cgttaccgct atcgtcgagc cccgggcgaa accgcgtcgg ccatcgcctg
     1201 ccgggcggcc cgggaagccc tcgacaaggc cggcctcgcc cccggggaca tcgacatgct
     1261 gatttacgtc ggcgtcggcc ggggggtctt ggaaccggcc acggccaatc tgttcaaaag
     1321 ccttctggga ttgacgaacg ccacctgttt cgacgtcttg gacgcctgcg ccagctgggt
     1381 gcggggcatg tccatcgccc agagtttcat caatcaaggc acccacaaaa ccgtcatggt
     1441 catgaacgcc gagctgaatt acgaagagta ttgcggcgac cacgccctgc gccggcccaa
     1501 agacctttat ttccggttcg cggcccacac catcggggaa gcggccacgg cgacgatcgt
     1561 ccgggacgac gggcgtcggg acaatttcta tttctccatg aaaaattggg aaaacaccta
     1621 cgatctctgc cagattcccc tgccgaaccg ggacgatttc gcgggcaatt atccgccccg
     1681 gaccccgcaa caaaacatgg tgttcgtcgc ggcgtcccgg gagctcctca cggccgccat
     1741 cacgaagctg acggaacagt tcaaggaaga tccccggctg aacggccaga tctacgacct
     1801 gcacttttgc cacgacgtca gcgactattc caccaagctg gtggccaagg ccctcaacgt
     1861 gcccctgtcc atggtgtacc gcacccacga gcgttttggg aacaccgtgt cggcgtcgat
     1921 ccccctggcg ctgtccctgg ccctggccga aggccgcctg aaaaaaggga tgaacctcct
     1981 gttgacctgc ggcagcgccg gcgtgaccac cgcctatctc caattccgtt atgactgacc
     2041 ggcggttccg gtgatcttcc gcgcccgcgt cgcccagcgg gggtggacgc gcatcctggc
     2101 ggcgggggtt ctcatgtacg cctacgccgc ggccttcgtc gtcgtcgacc tcctggcgcg
     2161 cttcggccag gcgaccgttc tccgtctctg cggaatcccg cccgtggacc gccgccgtta
     2221 catcgccttg gaccggggcc gcgtcccccc cttgaccccc ttcgacggat tcaattgcaa
     2281 ttactgcggc tacgccaacg gcgtcctggc ctgggcccgg gaaatcgtgc gccgggtgga
     2341 acagtattgg tgccccgtcc ggcacgccgc gccgctggac ggcgacgtcg acccggcgac
     2401 gtacgccccc gccggggacc gcgccgcctt ggaccgctgg ttggagcctc cccgcccgtg
     2461 acgggtcgcc ccccgtcggt ttcgaaggac ctttacccct tcgcttccca ttacctggag
     2521 cggaacggcc accggcttca ttacgttgac gaaggccggg gcgcccccgt gctcctgctt
     2581 cacggaaatc ccacctggtc cttttactac cgacgggtca ttgaggacct ccgcgtcgat
     2641 caccgggtca tcgctctgga ccacctcggg tgcggccttt ccgacaaacc ccaggattat
     2701 ccgtaccgcc tcgaaaacca catcgccaac gtggaatatt tggtcgacgc cctgggcctg
     2761 aaaaacctct ccttggttct gcacgattgg ggcggggtca tcggcgccgg gtacgcggtg
     2821 cgccaccccg ccaacgttgt tcggtgggcc ctgatgaaca ccgccgcttt ttggcccgcg
     2881 gggtacctgc cccttcgact gcggttgtgc cggattcccc gggtggggga gttcctgatc
     2941 cgtcggttca atctcttcgc gcgcatcgcc ctgcgggtgg ccgttcgccg tccgggcggg
     3001 ttgaccccgg aggttcggcg ggcctacctg gccccctacg actcctacgc ccaccggatc
     3061 gccacggcgc ggttcgtcct ggacgttccc gattcgcccc ggcaccccag ttacgccacc
     3121 gcccaggccg tcgaatcggg attggcccaa ctcgtccgac tgcccacgcg cattttct
//
LOCUS       DAOQLS010000182         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_16987_length_3143_cov_4.768459, whole genome shotgun sequence.
ACCESSION   DAOQLS010000182 DAOQLS010000000
VERSION     DAOQLS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16987_length_3143_cov_4.768459"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK362_04480"
     CDS             <1..399
                     /locus_tag="PK362_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74126.1"
                     /translation="AIIGLLAAIAIPKFANLVDKSKEAAARGKLGTMRSAMNLYYADN
                     EGGYPAGFLATGEPLVPRYLDKVPYVEMPRTVASGRHRGKFMASGGVPCCVDDWFSDF
                     ETAWHYSLVDHAVRLNCTHTDSKGTTWSLW"
     gene            587..904
                     /gene="groES"
                     /locus_tag="PK362_04485"
     CDS             587..904
                     /gene="groES"
                     /locus_tag="PK362_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC74127.1"
                     /translation="MVDAVLTKVKIRPLGDRVLVKPLDKEKQERGGLIIPDTAKEKPQ
                     EGEIVAAGKGKVSDDGKVLPMDVKAGDRILYGKYSGTEIKIDGDDYLIMHQDDILGIL
                     ESK"
     gene            919..2559
                     /gene="groL"
                     /locus_tag="PK362_04490"
     CDS             919..2559
                     /gene="groL"
                     /locus_tag="PK362_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561033.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNC74128.1"
                     /translation="MAKQIKYGDDARKAVKAGVDKLSDAVKATLGPKGRNVVLDKKFG
                     SPTVTNDGVTIAKDIELEDPFENMGAQLVREVSSKTNDVAGDGTTTACVLAQGIIAEG
                     FRNITAGANSTHIKRGIDKAVEAVVAELKKNAKKINIDAKAKDEITQIATISASDKEI
                     GQKIADAILKVGKDGVITVEEGKTSATIVKTVEGMQFDRGYISPYFVTDAERMEGVLD
                     DPLIIITDKKVAAMNDLLPLLEKILQQGKQFLLIAEDVEGEALATLVVNKIQGRIRCV
                     AVKAPGFGDRRKEMLEDIAILTGGQVITEEKGFKLDKAGIDMLGRATRVVVDKENTTI
                     VGGQGDEKTIKGRIAQIKKQIDETTSDYDKEKLQERLAKLSGGVAVIEVGAATETEMK
                     SKKFKVEDAMHATRAGVEEGIVAGGGVALLRAQKALDGLKGTDQDEDTGIKIIRRVLE
                     DPLRTIADNAGVDGSIVVDKVRTNADPHFGYNAESNEYGDLLKQGVVDPVKVTRCALQ
                     NAASVAGLLLTTDVLVTDIPEKKQPAPAMPGGMGGGDF"
     gene            2717..>3143
                     /locus_tag="PK362_04495"
     CDS             2717..>3143
                     /locus_tag="PK362_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74129.1"
                     /translation="MKVRICVALGLALTGAPLRAARLTLQPAVTVMGVDGIGRVNALY
                     DEDDDGPLPALRVDPSSVSWTSSQPGTVALNVGDPGTYSAVAAGESVVSGTYLGLTAS
                     GTIRVAGRVVSGSLVTPGDGRTRTFLVFQPSGYKPGSPRP"
BASE COUNT          717 a         1026 c          917 g          483 t
ORIGIN      
        1 gccatcatcg gcctcctggc ggccatcgcc attccaaaat tcgccaatct ggtcgacaaa
       61 tccaaggaag cggccgcccg gggaaaattg ggaacgatga gaagcgcgat gaatttgtat
      121 tacgccgaca atgagggggg gtatccggcc ggctttttgg ccacgggcga gcccctggtg
      181 cctcgttatt tggacaaagt gccttacgtg gaaatgccgc gaaccgtggc gtccggccgt
      241 caccggggaa aattcatggc gagcggcgga gtcccctgtt gcgtcgacga ttggttttcc
      301 gacttcgaaa ccgcctggca ctacagcctg gtggaccacg ccgtgcgtct caactgcacc
      361 cacaccgatt ccaagggcac cacttggagc ctttggtaac ccttgacagc gttcccccca
      421 atcaactaaa attatcacac accgaataag agtgctggtt ttgagttcct gaacccgctc
      481 tttatcgtcg gaaaagcgcc acaaggcccc tccgggtccg tggcttttta ttcgaaattt
      541 aattagcaat cgatttaatc gattgccagc aatggaggaa actagcatgg ttgacgcggt
      601 tctcaccaaa gtcaaaatcc gtcctctggg cgaccgggtc ctggtcaagc ccttggacaa
      661 agagaaacag gaacgcggcg ggctcatcat ccccgacacc gcgaaggaaa aaccccagga
      721 aggcgagatc gtcgccgcgg gcaaaggcaa agtgtccgac gacggcaaag tcctgcccat
      781 ggacgtcaaa gccggcgacc ggatcctcta cggaaaatat tccggcaccg agatcaagat
      841 cgacggggac gactacctga tcatgcatca ggacgacatc ctcggcattc tggaatccaa
      901 ataatttagg agactcccat ggccaaacaa atcaaatacg gcgacgacgc gcgcaaagcc
      961 gtcaaagccg gcgtggacaa actgtccgac gccgtgaaag ccacgctcgg ccccaagggc
     1021 cgcaacgtgg tgctggacaa aaaattcgga tcgcccaccg tcaccaacga cggcgtgacg
     1081 atcgccaagg acatcgagct ggaagacccc tttgaaaaca tgggcgccca gctggtccgc
     1141 gaagtgagct ccaagaccaa cgacgtggcc ggcgacggca cgacgacggc ctgcgtgctc
     1201 gcccagggca tcatcgccga agggttccgc aacatcaccg ccggcgccaa ctccacccac
     1261 atcaagcgcg gcatcgacaa agccgtggaa gccgtcgtgg cggagctcaa aaagaacgcc
     1321 aaaaaaatca acatcgacgc caaggccaaa gacgagatca cccagatcgc cacgatctcg
     1381 gcctcggaca aggaaatcgg ccagaagatc gccgacgcca tcctcaaagt cggcaaagac
     1441 ggcgtgatca ccgtggaaga gggcaaaacc tccgccacca tcgttaaaac cgtcgaaggc
     1501 atgcagttcg accggggcta catctccccc tacttcgtga ccgacgcgga gcgcatggaa
     1561 ggcgtcctgg acgaccccct catcatcatc accgacaaga aagtcgccgc catgaacgac
     1621 ctgctcccgc tcttggaaaa gatcctccaa cagggcaagc agttcctcct gatcgcggaa
     1681 gacgtggaag gcgaagccct ggcgaccctc gtggtcaaca agatccaggg ccgcatccgc
     1741 tgcgtcgccg tgaaagcccc gggcttcggc gaccgccgca aagaaatgct ggaagacatc
     1801 gccatcctga cgggcggcca ggtcatcacc gaagagaagg gcttcaagct cgacaaggcc
     1861 ggcatcgaca tgctgggccg cgccacccgc gtcgtcgtgg acaaggaaaa caccacgatc
     1921 gtcggcggcc agggcgacga gaagaccatc aagggccgca tcgcgcagat caagaagcag
     1981 atcgacgaaa ccacctccga ctacgacaag gaaaaactgc aagagcggct ggcgaagctc
     2041 tccggcggcg tggccgtgat cgaagtgggc gcggcgaccg agacggaaat gaagtccaag
     2101 aaattcaagg tggaagacgc catgcacgcg acccgcgcgg gcgtcgagga aggcatcgtg
     2161 gccggcggcg gcgtggcgtt gcttcgcgcc caaaaggccc tggacggcct gaaagggacc
     2221 gaccaggacg aagacaccgg gatcaagatc atccggcgcg tcctggaaga ccccctgcgc
     2281 accatcgccg acaacgccgg cgtggacggc tccatcgtcg tggacaaggt ccgcacgaac
     2341 gccgacccgc acttcggcta caacgccgaa tccaacgagt acggcgacct gctcaagcag
     2401 ggcgtcgtgg acccggtgaa ggtcacccgc tgcgcgcttc aaaacgcggc cagcgtggcg
     2461 ggcctcctcc tgaccaccga cgtgcttgtg accgacattc cggagaagaa acaacccgct
     2521 ccggcgatgc ccggtggaat gggcggcgga gacttctaaa agcccggacc accacgaggc
     2581 caagcgccgc aaggcgcgca tcgcattcca aaacgggcgg ggttcttcgg aaccccgccc
     2641 gttttttttc gtcccgcgac actttgacca cccccgaatc gtccaataga acatcggtcc
     2701 atcggccgga ggcgttatga aagttcgcat ttgcgtggcc ctgggtctgg cgctgaccgg
     2761 cgcccccctt cgcgccgccc gtttgaccct gcagcccgcc gtcaccgtca tgggggtgga
     2821 cgggatcggc cgcgtcaacg ccctctacga cgaagacgac gacggccccc ttccggccct
     2881 tcgggtcgac ccctcctccg tttcctggac gtcgtcccaa cccgggaccg tggccctgaa
     2941 cgtcggcgat ccgggaacgt actccgccgt ggccgcgggc gaatccgtcg tttcgggcac
     3001 ctacctgggc ctcaccgcct cggggaccat ccgcgtggcg ggtcgggtcg tctccggctc
     3061 tctggtgacc ccgggggacg gacgcactcg gacgtttctc gttttccagc cttccggcta
     3121 caagccgggt tcgccgcggc ccc
//
LOCUS       DAOQLS010000183         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17029_length_3139_cov_4.704929, whole genome shotgun sequence.
ACCESSION   DAOQLS010000183 DAOQLS010000000
VERSION     DAOQLS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17029_length_3139_cov_4.704929"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PK362_04500"
     CDS             <1..827
                     /locus_tag="PK362_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNC74130.1"
                     /translation="KFIDVMTALTQRPGEKTAAVMVQAVLEATGYWAHWEEQVDTDPE
                     AAHRLDNLQELVNAAKEFEDVSEDKSVPTFLEKVSLASDLDNLKAEGGAVTLMTVHLA
                     KGLEFPSVYLTGLEEGLFPIGESAFDEKELEEERRLAYVGITRARRFLTLTAASSRKI
                     YGRSNWHVPSRFVAEAGLTAERPVPSGLFESVEPAAAAPRPAAVASFDPDEGDTASPG
                     GQRPLRIGQRVRHPMFGEGKILDKNGSGENLKVTVLFDSGARKSILVRYANFEPTA"
     gene            844..1131
                     /gene="gatC"
                     /locus_tag="PK362_04505"
     CDS             844..1131
                     /gene="gatC"
                     /locus_tag="PK362_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNC74131.1"
                     /translation="MSLTEKDVNHVARLARLALTDAERKKYLGQLTRILDHIQTLSAY
                     NTDAVAATHHVAPLSNVWREDVAVPFADRESILANAPEREDVFFKVRKVIE"
     gene            1128..2597
                     /gene="gatA"
                     /locus_tag="PK362_04510"
     CDS             1128..2597
                     /gene="gatA"
                     /locus_tag="PK362_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNC74132.1"
                     /translation="MSAPHEWTAREIADRVKSRKISAQEVARAFLSRAKAWDKTVKAF
                     LSLDEEKALAQARSVDDRLARGENPGPLAGVPVAIKDNMCVTGTRTTCASKILENFVA
                     NYDATAIARLRDAGAVFIGKTNLDEFAMGSSTENSAYFTTKNPWDPTRVPGGSSGGSA
                     AAVAARLAPLALGSDTGGSIRQPAALCGIVGLKPTYGRVSRFGLVAFASSLDQIGPFA
                     WNTADAALLLGVVAGHDPRDSTSVQRPVPDYLARSADSIKGLRVGLPKEYFIDGMDPA
                     VEKAVREAVKTLESLGATVREVSLPHTDAGLSVYYVLAPSEASANLARFDGVRYGHRS
                     AKAANLLEQYELSRDEGFGPEVKRRIMLGTYALSSGYYDAYYLKAQKVRTLIKNDFDQ
                     AFQQVDVIVTPTAPTPAFKAGEKSDDPLQMYLSDVFTISCNLAGIPGLSLPCGFSGGL
                     PIGLQLLGRPFEEERVLAAARAYEEATAWVKTPPTPPGL"
     gene            2594..>3139
                     /locus_tag="PK362_04515"
     CDS             2594..>3139
                     /locus_tag="PK362_04515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC74133.1"
                     /translation="MIDPVGPRLSVVVPAYNEEKFLGPLLASLDAARARLKAERGVEA
                     EIIVVDNGSTDRTAEVARAAGARVVAESKRQIAASRNAGVRAARGTVVVTCDADNRVS
                     DNLLVRVDEEMARGDCVGGGVRIVPEESRWNTDLLFAVFDWGARLFGVGFGVLFTDRA
                     TFDRIGGFPETVYVGEDGFFVL"
BASE COUNT          536 a         1060 c         1006 g          537 t
ORIGIN      
        1 tgaaattcat cgatgtcatg accgccctga cccagcgccc gggggaaaaa accgcggccg
       61 tcatggttca ggccgttttg gaggcgaccg ggtattgggc ccactgggaa gaacaggtgg
      121 acactgaccc cgaggcggcc caccggctgg acaacctgca ggaattggtg aacgccgcca
      181 aggaattcga ggacgtctcc gaggacaagt cggtgccgac cttcctggaa aaggtctccc
      241 tggcctcgga cctggacaac ttgaaagccg agggcggcgc cgtgacgctc atgacggtcc
      301 acctggccaa ggggctggaa tttccgtcgg tttatttgac gggtttggaa gaaggcctct
      361 ttcccatcgg cgaatcggcc tttgacgaga aggagttgga ggaggagcgg cggctggcct
      421 acgtgggcat cacccgggcc cgccggtttc tgacgctcac cgccgcctct tcccgtaaaa
      481 tttacggccg ctccaactgg catgtgcctt cccgcttcgt ggccgaggcg ggcctcacgg
      541 cggaacgtcc ggtgccctcg ggccttttcg aatccgtgga gcccgccgcg gcggcccccc
      601 ggcccgcggc cgtggcgtcc ttcgacccgg acgagggcga caccgcgtcg ccggggggcc
      661 agcgccccct gcgcatcggc caacgggtgc gccaccccat gttcggcgag gggaaaatcc
      721 tggacaaaaa cggctccggc gaaaatttga aagtcaccgt gttgttcgat tccggggccc
      781 gaaaaagcat tctggtccgt tacgcgaatt tcgaacccac cgcgtaactt gtaaaatacg
      841 cccatgtccc tgaccgaaaa agacgtcaac cacgtggccc gcctggcccg gctcgccctg
      901 accgacgccg agcgtaaaaa atacctcggc cagctgaccc gcatcctgga ccacattcaa
      961 accctgagcg cctacaacac cgacgccgtg gccgccaccc atcacgtggc gcccctctcc
     1021 aacgtgtggc gggaggacgt cgccgtcccc ttcgccgacc gggagtcgat tctggccaac
     1081 gcgcccgagc gggaggacgt tttcttcaag gtgcgaaagg tcatcgagtg agcgcccccc
     1141 acgaatggac cgcccgggag atcgccgacc gggtgaaatc cagaaaaatc tccgcccagg
     1201 aagtggcccg ggcctttctc tcccgcgcca aggcctggga caagaccgtc aaagccttcc
     1261 tgtccctgga cgaggagaaa gccctggccc aggctcggtc ggtggacgac cggctcgccc
     1321 ggggggaaaa cccggggccc ctggccgggg ttccggtcgc gatcaaagac aacatgtgcg
     1381 tgacgggcac ccgcaccacc tgcgcctcca aaatccttga aaacttcgtg gcgaattacg
     1441 acgccaccgc catcgcccgc cttcgggacg cgggcgccgt cttcatcggc aaaacgaatc
     1501 tggacgaatt cgccatgggg tcctccacgg agaactcggc ctatttcacc accaagaacc
     1561 cctgggaccc gacccgcgtc cccggcggct cctcgggggg aagcgcggcc gccgtggccg
     1621 cccgtttggc gccgttggcc ctggggtccg acacgggcgg ctccatccgg caacccgccg
     1681 ccctctgcgg catcgtgggc ctcaagccca cctacggccg ggtctcccgt ttcggcctcg
     1741 tcgctttcgc ctcctccctg gaccaaatcg gccccttcgc ctggaacacg gccgacgcgg
     1801 cgttgctgtt gggcgtggtg gcgggccacg atccccggga ctcgacctcg gtccagcgtc
     1861 ccgtgcccga ttacctggcc cgatcggcgg attccatcaa aggccttcgc gtcggactgc
     1921 ccaaggagta tttcatcgac ggcatggacc ccgcggtgga gaaggccgtc cgggaggccg
     1981 tcaaaaccct cgagtccctg ggggccacgg tgcgcgaagt ctccctgccc cacaccgacg
     2041 cgggcctctc ggtttattat gtcttggcgc cttccgaggc cagcgccaac ctggcgcgtt
     2101 tcgacggcgt gcggtacggc caccggtccg ccaaggccgc caatttgctg gagcaatacg
     2161 agctctcccg ggacgagggc ttcggcccgg aagtcaaacg gcggatcatg ctcgggacct
     2221 acgccctgtc ctcgggttat tacgacgcct attacctcaa ggcccaaaag gtccgcacct
     2281 tgattaaaaa cgatttcgac caggccttcc aacaagtcga cgtgatcgtc accccgaccg
     2341 cccccacgcc ggcttttaag gcgggggaaa agtccgacga cccgcttcaa atgtatttgt
     2401 ccgacgtttt tacgatttcc tgcaatttgg cggggattcc gggcttgtcc ctgccctgcg
     2461 gtttttccgg cgggctgccc atcggccttc agctcctggg gcgccccttc gaggaagagc
     2521 gggtgttggc cgccgcccgg gcctacgagg aagccaccgc ctgggtcaaa acgccgccga
     2581 cgccgccggg cctttgatcg acccggtcgg gccgcgcctc tcggtcgtgg tgccggccta
     2641 caacgaggaa aagtttttag gccccctgtt ggcttccctg gacgcggccc gggcgcgatt
     2701 aaaggccgaa cggggcgtcg aggccgaaat catcgtcgtg gacaacgggt ccaccgaccg
     2761 cacggcggag gtggcccgcg cggccggggc ccgggtcgtg gcggaatcga agcggcagat
     2821 cgccgcttcc cgaaacgccg gcgtccgcgc cgcccgggga acagtcgttg tcacctgcga
     2881 cgccgacaac cgggtgtccg acaatttgct ggtccgggtg gacgaggaga tggcccgggg
     2941 cgattgcgtg ggcggcgggg tgcgcatcgt gccggaagaa agccgttgga acacggattt
     3001 gttgttcgcc gttttcgatt ggggagcccg gctcttcggc gtggggttcg gcgtgctgtt
     3061 cacggacagg gccaccttcg accggatcgg gggctttccg gagacggtgt atgtgggcga
     3121 ggacgggttc ttcgtgctg
//
LOCUS       DAOQLS010000184         3130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17123_length_3130_cov_4.092033, whole genome shotgun sequence.
ACCESSION   DAOQLS010000184 DAOQLS010000000
VERSION     DAOQLS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17123_length_3130_cov_4.092033"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PK362_04520"
     CDS             <1..904
                     /locus_tag="PK362_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC74134.1"
                     /translation="FIGSHVAAAYLRAGHAVTVLDNLSHGRRGAVPSGARFVKADVRG
                     PGLASIFARGRFDVVNHHAAQIDVRRSVADPLEDADINLRGLLQLLENSRAHRVRKFI
                     FAASGGTYYGECARPARESDPPRPLSPYGVSKLSSEFYLRAYRALHGLDFTVLRYANV
                     YGPGQDPHGEAGVVAIFCQRLSAGKPVTVFGSGRQERDYVFVGDVAEANRRALTRGSG
                     GCFNIGSGRATSVQELLRALREVSGRSSPVVRRPARPGELKRSCLDFGLATRVLGWRP
                     GVSLREGLAETWRRHRSTEERFRP"
     gene            901..1980
                     /locus_tag="PK362_04525"
     CDS             901..1980
                     /locus_tag="PK362_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNC74135.1"
                     /translation="MKALILLGGLGTRLRPLTLTRPKPLLPVLNRPLIAHQLDALRGM
                     GIRNVVLALGYKAAHFKALLGRGNRWGVRFHYSIERDPLGTGGAIRKALGSMDGTTMI
                     LNGDILSDIDLRGLTARHARAKAQATLALTEVKDPSAFGLVQTDPGGRVVGFLEKPSA
                     EQTTCRTINAGVYVFEPEIVQRIPAGRPVSIEREIFPALIAEGYRVRAEVHRGYWSDV
                     GTLAAYWRTHADLHLLGRWPAGYRVRGGLVRGPGVRWHPTARQRGTAVIGRGSRVDRG
                     AVLEGCVTLGDRVRVGAGARLVNCVVLEGAVIGDRSRVENAVLGAGTVLGADCRVGPD
                     LVMGDGARWPDHSQCVPGLTDGAPN"
     gene            2085..>3130
                     /gene="metK"
                     /locus_tag="PK362_04530"
     CDS             2085..>3130
                     /gene="metK"
                     /locus_tag="PK362_04530"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNC74136.1"
                     /translation="MRRDRYVFTSESVTEGHPDKVCDQISDAVLDAILAEDPMGRVAC
                     ESFCTTGLVVVGGEITTKSWVDVPSLVRSVVSEIGYTDKRFGFDAASCGILNAIGRQS
                     PDIAQGVDTGGAGDQGLMVGFACDETEELMPTPISLAHKLTRRLADVRKKGVLPYLGP
                     DGKSQVTVEYEGGQPKRIEAVVLSSQHDESILDKSGKKITGRAREEMIAKVVLPVLPK
                     AMLDKDTKFYVNPTGKFVVGGPEGDTGVTGRKIIVDTYGGWAPHGGGAFSGKDPTKVD
                     RSACYMARHVAKNIVAAGLAQQCTVQLAYAIGVADPVSVMIDTHGTGAVPEERLVEMA
                     RDVFPLTPRGIIDY"
BASE COUNT          504 a         1007 c         1060 g          559 t
ORIGIN      
        1 gttcatcggg tcgcacgtgg cggcggccta ccttcgggcg gggcacgcgg tgaccgtttt
       61 ggacaacttg tcccacggtc gccggggggc cgtcccgtcg ggagcccggt tcgtcaaggc
      121 ggacgtccgg gggccgggcc tggcgtcgat tttcgcccgg ggtcggttcg atgtcgtgaa
      181 ccaccacgcc gcgcaaatcg acgttcgccg ttcggtcgcc gatcccttgg aggacgccga
      241 catcaatctg cgggggctcc ttcaattgtt ggagaattca cgcgcccatc gggttcgaaa
      301 atttattttc gccgcttccg gcggaaccta ttacggcgaa tgcgcccgtc ccgcccggga
      361 aagcgatccc ccgcgccccc tgtcgcccta cggcgtgtcg aaattgtcga gcgaatttta
      421 ccttcgggcc taccgcgccc ttcacggatt ggatttcacc gtgctccgct acgccaacgt
      481 gtacggaccg gggcaggacc cccacggcga ggccggggtc gtggccatct tctgccagcg
      541 gctgtccgcg gggaaacccg tcaccgtttt cggaagcggc cgccaggaac gggactacgt
      601 tttcgtgggg gacgtggccg aggccaatcg ccgggccctg acccggggtt cgggcgggtg
      661 tttcaacatc ggatcgggac gcgccacctc ggtccaggaa ctccttcggg ccctgcggga
      721 agtgtccggt cgctcttcgc ccgtggtccg acgcccggcc cgcccggggg agttgaagcg
      781 cagttgtctg gatttcggtt tggcgacccg ggtcttgggc tggcgcccgg gggtttccct
      841 tcgggagggg ttggccgaaa cctggcgtcg ccaccggtcg acggaggaac gattccgacc
      901 atgaaagcgc tgatccttct ggggggactg gggacccggc ttcgccccct gacgttgacg
      961 cggcccaaac cgcttttgcc ggttctcaac cgacccttga tcgcccacca attggacgcc
     1021 ttgcgcggca tgggaattcg gaacgtggtc ttggccctgg gatacaaagc ggcgcatttc
     1081 aaggcgctcc tgggccgcgg aaaccggtgg ggcgtccgtt tccattattc catcgagcgg
     1141 gatcccctgg gcaccggggg ggccattcgg aaagccctcg gttcgatgga cgggaccacg
     1201 atgatattga acggggacat tctcagcgac atcgatttgc ggggtttgac cgcccggcac
     1261 gcccgcgcca aagcccaggc caccctggcc ttgacggagg tcaaagaccc gtcggccttc
     1321 gggttggttc aaaccgaccc cggcggccgc gtcgtcggct ttttggaaaa gccgtcggcg
     1381 gagcaaacca cctgccggac gatcaacgcc ggcgtttacg tgttcgagcc cgagatcgtc
     1441 cagcggatcc ccgcggggcg gcccgtttcc atcgaacggg aaatctttcc cgctttgatc
     1501 gccgaagggt accgggttcg ggcggaggtg caccggggct attggtccga cgtgggcacc
     1561 ctcgcggcct actggcgaac ccacgcggat ttgcacctac tgggccgttg gccggcgggt
     1621 tatcgggtcc ggggcgggtt ggttcggggg ccgggggttc gctggcaccc caccgcccgt
     1681 cagcggggca ccgcggtgat tgggcgcggg tcgcgggtcg accggggcgc cgtcttggaa
     1741 ggttgcgtca ccctcggcga ccgcgtgcgg gtgggcgcgg gcgcgcggtt ggtgaattgc
     1801 gtggtgttgg aaggggccgt gatcggggat cgctcccggg tggaaaacgc cgtcctgggc
     1861 gccggaacgg tcctcggggc cgattgccgg gtgggtcccg acctcgtgat gggcgacggc
     1921 gcccggtggc cggaccacag ccaatgcgtg cccgggctca ccgacggggc cccgaattag
     1981 ggggggcgat cgttatttcc atacgcaccg ggcgcccccg aaattgataa aattcctcct
     2041 cttttccaag ttgacatcga ccattcacag ggagaaacca tcgtatgcgc cgtgaccgtt
     2101 acgttttcac ctccgagtcc gtgaccgaag gacatccgga caaggtttgc gatcaaattt
     2161 cagacgcggt gttggacgcg atcctggccg aggacccgat gggccgcgtg gcctgcgagt
     2221 ccttctgcac caccggcctc gtggtcgtgg gcggcgaaat cacgaccaaa tcctgggtgg
     2281 acgttccctc cctggtccgc tcggtggttt ccgagatcgg ctacacggac aaacggttcg
     2341 gtttcgacgc ggccagttgc ggtattttga acgccatcgg ccgccaaagc cccgacatcg
     2401 cccagggggt cgacaccggg ggcgccggcg accagggcct catggtcggc ttcgcctgcg
     2461 acgaaaccga ggagctcatg cccacgccca tcagcctggc gcacaagctc acccggcggt
     2521 tggcggacgt tcgtaaaaag ggcgtcctgc cttacctcgg ccccgacggg aaatcccagg
     2581 tgaccgtgga atacgagggc ggccagccga aacgcatcga ggcggtcgtc ctctccagcc
     2641 agcacgacga atccattttg gacaaaagcg ggaaaaaaat caccggccgc gcccgggagg
     2701 aaatgatcgc caaggtcgtc ctgccggtcc ttcccaaggc catgttggac aaagacacga
     2761 agttttacgt gaaccccacc ggcaagttcg tggtcggcgg tcccgaaggg gacaccggcg
     2821 tcacgggccg gaaaatcatc gtggacacct acggcggttg ggccccccac ggcggcggcg
     2881 ccttctcggg caaagacccg acgaaagtcg accgctccgc ctgttacatg gcgcgccacg
     2941 tggccaaaaa catcgtcgcc gcgggcttgg cccaacagtg cacggtccaa ctggcctacg
     3001 ccatcggcgt ggccgatccg gtgagcgtga tgatcgacac ccacggcacc ggcgcggtgc
     3061 ccgaggaacg cctggtggag atggcgcggg acgtctttcc gctcacgccc cggggcatca
     3121 tcgattattt
//
LOCUS       DAOQLS010000185         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17449_length_3098_cov_3.726586, whole genome shotgun sequence.
ACCESSION   DAOQLS010000185 DAOQLS010000000
VERSION     DAOQLS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17449_length_3098_cov_3.726586"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PK362_04535"
     CDS             <1..451
                     /locus_tag="PK362_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74137.1"
                     /translation="PLVYGPGAPGNFARLLRWARSGFPIPLGALTARRSFVFVGNLAS
                     AVLRALQHPRAANRPFLVSDGADLSTGDLLRALRAAGVPLRLVSIPAGPLRLLARWVG
                     RAGEFGKLADPLELDIGAIRRALEWRPPWTVTEGLARSVRPESVGGK"
     gene            533..1519
                     /locus_tag="PK362_04540"
     CDS             533..1519
                     /locus_tag="PK362_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC74138.1"
                     /translation="MTDWRWDGRMAASGTVFLAVLVFHAVLLRWKHRLPVDHPNDRSL
                     HSTPTPRIGGLALIPGLLLGVGVLGRGMSPLLGLAAALGLFSFLDDRFGLPVTLRLAI
                     HLGAAVVFTGLGGFWSDLRWGIALTFLIVWGTNLFNFMDGSDGLAGGMAFFGFAAYGA
                     AAGGADHPSGLLASGIAAGAAGFLLFNFPPARVFLGDAGSIPLGFLAAAMGVEGWARG
                     RWPAWFPALVFAPFWVDATVTLVRRTLRGEPVWRAHRTHYYQRLIQMGWGHRKMALVA
                     YGLMALCAAGGVALRRASAFAQRASLACAAVGAVVVMADVDRSWRRRAESSR"
     gene            1516..>3098
                     /locus_tag="PK362_04545"
     CDS             1516..>3098
                     /locus_tag="PK362_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNC74139.1"
                     /translation="MNFKKFLVFLHDVAATAVAWYAAFLLRFNFAIPPANLESLLWSM
                     AIVVPLQAVVFWWYGLYRSLWRFASLPDLRRIVMAVFVSAGASATALFLYRRLDQVPR
                     SVLVLDPILLVCLMGASRFLYRSWKDGHLIPFRKLATTPVLVLGAGRVASMLLRELAQ
                     DGQWRVVGLMDDDVTKKGRLLSGVPVLGPISELERHARERGVHQVILAIPSASPARRR
                     ALVETAAGLGLTVLTVPTMDDLLTGRISVSRVRRVELEDLLGRGRVKLNDAGLHELLT
                     DHTVLVTGAGGSIGAELCRQIARFSPHLLILYEANEYALYQVEQEFGERFPATPLAAL
                     AGDVRDAARLEEVFQRFRPEVVFHAAAYKHVPLMENDNCWEAVRNNVLGTYRIARASM
                     QAGVRKFVLISSDKAVNPTNVMGATKRMAEMVCQGLQSPGGETRFVVVRFGNVLGSTG
                     SVIPKFREQIAKGGPVTVTHPDITRFFMLIPEAAQLVLQAGYMAQGGEICVLDMGLPV
                     RILDLAKDMIRLSGFNHDE"
BASE COUNT          397 a          964 c         1059 g          678 t
ORIGIN      
        1 gccgctggtc tacggtcccg gggcgcccgg caatttcgcc cgcctgcttc gctgggcccg
       61 gtccggattt ccgattcccc tgggagccct gacggcccgc cgatccttcg tgttcgtcgg
      121 caatttggcg tcggccgtcc ttcgggcgct gcaacatccg cgggccgcga accggccttt
      181 tctcgtgagc gacggcgcgg atttgtccac gggcgatctc cttcgggcgc ttcgggccgc
      241 cggggtgccg ttgcggttgg tctccattcc cgccggcccc ctccggcttc tggcgcgatg
      301 ggtcggccgg gccggggaat tcgggaaatt ggcggacccc cttgaattgg acatcggcgc
      361 cattcgccgg gccttggaat ggcgtccgcc ctggacggtc acggagggtc tggcccggtc
      421 cgttcgtccg gagtcggtcg gggggaaatg atccgccggg cgtcgagtcc ccttggtata
      481 atgaacggcg ccgggcgaac cggcggggac ttggggtcga gggaggattg acgtgacgga
      541 ttggcggtgg gacggccgga tggccgcgag cgggacggtg ttcctggccg tgttggtttt
      601 tcacgccgtc ctgttgcggt ggaagcaccg gcttcccgtg gatcatccca acgaccgttc
      661 ccttcattcg acgccgaccc cccgcatcgg gggcctggcg ctgatccccg gattgctttt
      721 gggggtgggc gttttgggcc gggggatgtc gcctctcctg ggtctcgccg cggcccttgg
      781 cttattttcc tttctggacg atcgatttgg attgcccgtg accttgcgcc tcgcgattca
      841 tctcggcgcg gccgtcgtct tcaccggcct cggcggattt tggtccgacc tccgctgggg
      901 gatcgccttg acgttcttaa tcgtgtgggg caccaatctt tttaatttca tggacggctc
      961 ggacggcttg gccgggggca tggctttttt cgggtttgcc gcctacggcg cggcggccgg
     1021 gggggcggat catccctccg gactcctggc gtcgggaatc gcggccggag cggccggatt
     1081 cctccttttc aattttccgc ccgcccgggt gtttctcggc gacgcgggat ccatccccct
     1141 gggattcctc gcggcggcca tgggggtgga gggatgggcc cgggggcgtt ggcccgcctg
     1201 gttcccggcc ctggtgttcg cgcccttttg ggtggacgcc accgtgacgc ttgttcggcg
     1261 gacccttcgg ggcgagcccg tctggcgggc ccatcgaacc cattactatc aacggctgat
     1321 tcaaatggga tgggggcatc ggaaaatggc gttggtggcc tacggcctca tggccttgtg
     1381 cgcggccggc ggggtggcgc ttcggcgcgc ctccgccttc gcccaacggg cgtccctggc
     1441 gtgcgcggcc gtcggggccg tggtcgtcat ggcggacgtg gaccggagct ggcgccgccg
     1501 ggcggagagt tcgagatgaa tttcaagaag tttctcgttt ttcttcacga cgtcgccgcg
     1561 acggcggtgg cgtggtacgc ggccttcctc cttcggttca atttcgccat cccgccggcc
     1621 aacctggaaa gtctgctttg gtccatggcc atcgtcgttc ccctccaggc cgtcgtcttt
     1681 tggtggtacg ggctctaccg aagcctttgg cggttcgcga gcctccccga ccttcggcgc
     1741 atcgtgatgg ccgtcttcgt ttcggcgggg gcctccgcca cggccctgtt cctttaccgg
     1801 cgcctcgatc aagtgccccg gtcggtcctg gtgttggatc ccattcttct cgtttgcctc
     1861 atgggcgcca gccggtttct ctaccgttcc tggaaagacg ggcatttgat tccctttcga
     1921 aaactggcga ccacgcccgt tctggttctg ggcgccggcc gcgtggcgtc gatgttgctt
     1981 cgggaattgg cccaggacgg tcagtggcgc gtggtcgggc tcatggacga cgatgtcacc
     2041 aaaaaagggc gtcttctctc cggcgtcccg gtgttgggtc ccatttcgga attggagcgg
     2101 cacgcccggg aacgcggcgt tcatcaagtc attttggcca ttccctcggc gtccccggcc
     2161 cgtcgccgcg ccctcgtgga aacggcggcg gggctggggt tgacggtctt gaccgtgccc
     2221 accatggacg atttgctcac gggccgcatt tccgtctccc gggtccgccg cgtggagttg
     2281 gaggatttgt tgggccgcgg ccgcgttaaa ttgaacgacg ccggccttca cgaactgctc
     2341 accgatcaca cggtcctggt gacgggggcg ggcgggtcca tcggggccga actgtgccgt
     2401 cagatcgccc gattttctcc ccacttgttg atcctctacg aggcgaacga atacgccttg
     2461 tatcaggtgg agcaggaatt tggggaacgg ttcccggcga cgcccctggc cgccctggcg
     2521 ggggacgtcc gggacgcggc gcggttggag gaagtgttcc agcggttccg gcccgaggtc
     2581 gtgttccacg ccgccgccta caaacacgtg ccgctcatgg aaaacgacaa ttgttgggag
     2641 gccgtacgca acaacgtcct gggaacctac cggatcgcgc gggcctcgat gcaggcgggg
     2701 gttcggaaat tcgtcctcat ttcctccgac aaagcggtga accccaccaa cgtcatgggc
     2761 gccacgaaac ggatggcgga aatggtgtgc caaggccttc aatcgcccgg gggcgagacg
     2821 cggttcgtgg tggtccgttt tggaaacgtt ctcggcagca ccggaagcgt cattcccaag
     2881 tttcgcgagc aaattgccaa gggcggaccg gtgacggtca cgcacccgga catcacgcgt
     2941 ttttttatgc tgattcccga ggcggcccaa ttggttctcc aggcgggcta catggcccag
     3001 gggggtgaaa tttgcgtgtt ggacatgggt ttgcccgtgc gcattttgga tttggccaag
     3061 gacatgatcc gtctttcggg gttcaaccac gacgagat
//
LOCUS       DAOQLS010000186         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17486_length_3095_cov_3.390461, whole genome shotgun sequence.
ACCESSION   DAOQLS010000186 DAOQLS010000000
VERSION     DAOQLS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17486_length_3095_cov_3.390461"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3095
                     /locus_tag="PK362_04550"
     CDS             <1..>3095
                     /locus_tag="PK362_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74140.1"
                     /translation="LNGLLETVARAAEIPRGGAIGRRVALHSVRRRDDGTFLFSLQRP
                     NGTRGSLPDVLAEPTRYSVQGSLRRLRQTVQLRGFRPTVDPLGGFANLLNGRRLPAPA
                     VYRFDEAPFDLRGLAYAGSLVHEPAVALHRDLLQHSQGVRDVAIFHEFLEYLSARNYL
                     TYQLDETRKSLRVEINVPVRWHTFKSAWDISLTPEALELARRGAGQVHYFHRAVTRAT
                     LEDLDRELTETIRESVRQRLAPVDQSPAARARRDLEIVYRRFRQNTRNERFYELMAEW
                     LHQTGVGRSDAALAMARMEFRTDRPGVEIDRIGTLADPDLLERRPFGTGALRAGDVVV
                     APFIPTQMVRQYREFSGDRSWSPDDARPAAWLEEFLGALPAGVVLVSIDAPLKTVFQN
                     LRGTDIETALRNQGLWNRTFAHVLQDTGWYEDRLTRTLPEGAAGTDFSTTGVDRVVLA
                     NGASLSLHVKRSERLPPGPRGRPGVVVSLSSRTGADGTLASIHTRRKTENVLRGWGFR
                     PSDRTVRLVAALGVAWWYEFPRTLNVARLADFVYEHPGPRWIRVAGLAAIYATMGWAF
                     LETLQFLAGQANLVRFGAGLAAALLANPLGHLAWNLVFVWTGWELTHGSAIPAVPIRA
                     AGRSRSGWRVKTDLSALEFRNRHTAPESWTPISLKAEDNGNSALSFLRRSYELFPRSA
                     RREGPEAPSTSEVAYGRVVKALESLGALAPRLLSYDPGVRFETPGHPELFGLRAFSHW
                     MRKGDGDLVGAPEGLSFVVLPAELFSDPALAPADRRELLGTAVLIEAVKILRLAGRLT
                     LTLERDERGPWLALSVTNAQGAVETSRFMVYGEVARILSHHQDFHHLARALAAYWIPT
                     SEKRLNDFLIARGLRGYVEVVPPAASAAARPRAPWGRAAAAILVGLALSFGVPGTDFM
                     STAAAATLSAASPLRWIDPSFVAAALTLIMLSAASTWEPLRAILGRWPDRLAAAKDTI
                     LMMATSRGPPGGRGGRNGGTPGPRVPPREKAEILFRIDGQIAVARNAGGPKRDRRCAR
                     R"
BASE COUNT          440 a         1067 c         1032 g          556 t
ORIGIN      
        1 gttgaacggt ttgctggaga ccgtcgcccg ggccgcggaa attccccggg gcggggccat
       61 cgggcggcgt gtcgcccttc attcggtccg ccggcgcgac gacggcacgt tcctgttttc
      121 tcttcaacgg ccgaacggga cccggggaag tctgccggac gttttggccg aaccgacccg
      181 ctactccgtt cagggatcgc tgcgtcgact tcggcaaacg gtccagttgc ggggcttccg
      241 tccgaccgtc gacccgctcg gcggcttcgc caatctcttg aacggccggc ggctgcccgc
      301 gccggcggtc taccgtttcg atgaggcccc cttcgacctc cgggggttgg cttacgcggg
      361 aagcctcgtc cacgaaccgg cggtggccct gcatcgggat cttctccaac actcccaggg
      421 cgtccgggac gtggcgatct tccacgaatt cctggaatat ctttcggccc ggaattattt
      481 gacttaccaa ttggacgaaa cccggaagtc cctgcgcgtg gaaatcaacg ttcccgtgcg
      541 ctggcatacc ttcaaatcgg cctgggacat ctccctgacc cccgaggcgc tcgagttggc
      601 gcggcgcggg gcgggccagg tccactactt ccaccgggcc gtgacccggg ccactctgga
      661 ggacctggat cgggagctga ccgaaaccat ccgggaatcc gtccgccagc ggctggcgcc
      721 ggtggaccaa tcccccgcgg cccgcgcccg tcgggacctg gaaatcgttt atcggcgatt
      781 ccgacaaaac acccggaacg aacggtttta cgaattgatg gcggagtggc tccaccaaac
      841 gggggtgggc cggtcggacg cggccctcgc catggcccgg atggaattcc ggacggaccg
      901 tcccggcgtc gaaatcgacc gcatcgggac cttggccgac cccgacttgc tggaacgccg
      961 gccgttcgga accggcgctt tgcgggcggg cgacgtggtg gtcgcgcctt ttattcccac
     1021 ccagatggtc cgccaatacc gggaattttc cggcgatcgg tcctggtctc ccgacgacgc
     1081 gcgcccggcc gcctggctgg aggaattttt gggtgccttg cccgccgggg tcgtcctggt
     1141 gtcgatcgac gcgccgctca aaacggtctt tcagaatttg cgggggacgg acattgaaac
     1201 ggcccttcgg aaccaggggc tgtggaaccg gactttcgcc cacgtcctcc aggacaccgg
     1261 atggtacgag gacagactca cccggacgct tcccgaggga gcggcgggca cggatttctc
     1321 cacgaccggg gtcgaccgcg tcgtcctggc caacggggcc tccctttccc tgcatgtgaa
     1381 acggtccgag cgccttcccc cgggtccccg gggccggccg ggcgtggtgg tgtccctgtc
     1441 ctcccggacg ggcgccgacg gaaccctggc ctccatccac acgcggcgca aaaccgaaaa
     1501 cgttctacgc ggctggggat tccgtccctc ggaccggacg gtccgcctgg tggccgccct
     1561 cggcgtggcc tggtggtatg aatttccccg gaccctcaac gtggcccggt tggccgattt
     1621 cgtttacgaa caccccgggc cccggtggat tcgcgtggcc ggtttggcgg cgatttacgc
     1681 gaccatgggg tgggcgttcc tggaaaccct ccagttcctg gccggccagg ccaatctcgt
     1741 gcggttcggg gcgggcctgg cggcggcgct cctggccaat cccctgggcc acctggcgtg
     1801 gaacctcgtg ttcgtttgga cgggatggga actcacccac gggtcggcca ttcccgccgt
     1861 gcccatacgt gcggccggcc gctcccgcag cgggtggcgc gtaaagacgg atttgagcgc
     1921 gctggaattc cgaaaccgcc acacggcgcc ggaatcctgg acccccattt ccctcaaagc
     1981 cgaggacaac ggaaattccg ccctgtcgtt tcttcggcgg tcttacgaac tcttcccccg
     2041 ctcggcccgg cgggaagggc ccgaagcccc gagcacttcg gaagtcgcct acgggcgcgt
     2101 ggtcaaagcg ttggaatccc tgggggccct ggcgccccgg ctcctctcct acgaccccgg
     2161 cgtgcggttc gagaccccgg gccacccgga gcttttcggt ctgcgggcct tctcccactg
     2221 gatgcggaag ggcgacggcg atttggtggg cgcgccggag gggctctcct tcgtcgtcct
     2281 tccggccgaa ctcttttcgg accccgccct cgccccggcc gatcgccggg aattgctcgg
     2341 aacggccgtt ttgatcgagg ccgtcaaaat tcttcgactg gccggtcgcc tgacgctgac
     2401 gctggagcgc gacgagcggg ggccctggtt ggcgctctcc gtgacgaacg cccaaggggc
     2461 ggtggaaact tcgcgcttta tggtgtacgg cgaagtggcc cggattttga gccaccacca
     2521 ggatttccac catttggccc gggccctggc ggcctattgg attccgacct cggaaaaacg
     2581 gttgaacgat ttcctgatcg cccggggcct gcggggctat gtggaagtcg ttccgccggc
     2641 cgcctccgcg gcggcccgtc cccgggcgcc ctggggccgc gcggccgcgg cgattctggt
     2701 cggactggcc ctctccttcg gcgtgcccgg aaccgatttc atgtccacgg ccgcggcggc
     2761 cacgctatcg gccgcttccc cgctccgttg gatcgacccg tcctttgtcg ccgcggcgtt
     2821 gacgctgatt atgctttcgg ccgcgtcgac ctgggagccc cttcgcgcga ttctcggccg
     2881 gtggccggac cgtttggcgg cggccaagga tacgattttg atgatggcca cgagccgcgg
     2941 gcctccgggc ggccgcggcg gccgaaacgg cgggaccccc ggcccccgcg tgcccccgcg
     3001 ggaaaaagcc gaaatcctgt tccggatcga cgggcaaatt gccgtcgcgc ggaacgcggg
     3061 cgggccgaag cgagacagac gttgcgcgag gcgca
//
LOCUS       DAOQLS010000187         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17508_length_3093_cov_5.186965, whole genome shotgun sequence.
ACCESSION   DAOQLS010000187 DAOQLS010000000
VERSION     DAOQLS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17508_length_3093_cov_5.186965"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK362_04555"
     CDS             complement(<1..307)
                     /locus_tag="PK362_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74141.1"
                     /translation="MKRSPTKYERRSFVFQVRSGVGEDPAEILTHLTHAVWRALEKRT
                     GPGAVDPHQWPDVFRTLRDAFKNRLSYNPRCGQWDHCRRAPCAFCGKESHDVPAAPKI
                     "
     gene            complement(469..1980)
                     /locus_tag="PK362_04560"
     CDS             complement(469..1980)
                     /locus_tag="PK362_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNC74142.1"
                     /translation="MSDRRFPEHFYNWISLAGAALAVGTFSIIVLLILIDFFIQETTL
                     YLGMLTYMILPGFLVAGLAMVLGGALWERRRRARGLSASLGTSFHIDLQNPQTRRVLM
                     IGVAVTSVFLVASSLGTYKAYHISESTKFCGTLCHSVMSPEYTAYQRSSHARVACVAC
                     HIGPGADWFVKSKLTGAYQVYSTMFHKYARPIETPIKNLRPARDTCLQCHWPEKFFGE
                     RKHVNPHYLADEKNTPYPITLLVKIGGGSANAKEGSGIHWHMMISNKLEYIARDRARQ
                     DIAWIRATSKDGRVKIYENPDNALTDAEKAKAEIRTMDCIDCHNRPSHNYRPPQREVN
                     AALAAGEIPTNLPFIKREAVKALDAEYPTTPAARAAIADKIRGFYEKEYPAVWAARRE
                     DVERAVRAVQTIHAQNFFPEMRVTWRAYPENIGHSLAAGCFRCHGTDLKTSAGETITK
                     DCTTCHTILSQGTGPGADTVSVKGLPFRHPQDIGGMELEGQCNACHQGGAELY"
     gene            complement(2009..>3093)
                     /gene="nrfD"
                     /locus_tag="PK362_04565"
     CDS             complement(2009..>3093)
                     /gene="nrfD"
                     /locus_tag="PK362_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNC74143.1"
                     /translation="AVTAVVLVIRFARGMGAMTHMTDANPWGVWVGFLQAGVALSGGG
                     FVMAATVHIFHIKRFEPILRPVVLLAFLGYVFVALTLFIEVGRPYNLWHPLAMWQHHS
                     IMFEVAWCVTLYLTVLALEFSPAVLERLGYTRAVEALHRAAIPVVILGVILSTLHQSS
                     MGTMFLIVPQKIHPLWSSTLLPVFFLVSSVAVGLCVTVVASFYSARLFEKSLDRWLLA
                     DLARAASMVLFLYTALRLVDVSARNAWARFGDPAWLGGVFLLEVGGGALLPAVLLSRR
                     AVRDRPRWTVAACLPVLAGVVLNRLTVSWLAMVPYTGPGYWPHWMEVSVSFALLTGVA
                     AVFGLAVRTLPVFPAANPPAPASGAV"
BASE COUNT          627 a          969 c          992 g          505 t
ORIGIN      
        1 aaattttcgg agcggcgggc acgtcgtggg actcttttcc gcaaaaggcg cacggcgccc
       61 ggcgacaatg atcccattgg ccgcaccggg ggttgtagga caggcggttt ttgaaagcgt
      121 cccgcagggt gcggaacacg tcgggccatt ggtgggggtc cacggcgccg ggcccggtac
      181 gcttctccag ggcccgccac accgcgtggg tgaggtgggt gaggatttcg gcggggtctt
      241 cgcccacgcc ggagcggact tgaaaaacga aggaacgccg ctcgtatttc gtcggagatc
      301 gtttcacggg gccccctttg accagcaccg ggagagccgc ggggagtggg cgccgttgcc
      361 ctcgctctta ttgaatcaca accgcgcggg ggcggccatg atcgtcgtca tgtccgacgc
      421 gatgattcgg tcggggtccc ggcggaaccc ggtcgggaga ggccccgctc aatacaactc
      481 ggcccctcct tggtgacagg cgttgcattg cccttcgagt tccattccgc cgatgtcctg
      541 cgggtggcgg aagggaaggc ctttcacgga aaccgtgtcg gccccggggc cggttccctg
      601 ggagaggatc gtgtggcagg tggtgcaatc cttggtgatc gtttcgcccg ccgacgtctt
      661 caggtccgtg ccgtgacagc ggaagcaacc cgccgccagc gagtggccga tgttttccgg
      721 ataggcccgc caggtcaccc gcatttcggg aaagaagttt tgggcgtgga tggtttgcac
      781 ggcccgaacg gcccgttcca cgtcctcccg gcgagcggcc cacaccgcgg gatactcctt
      841 ttcgtaaaac ccgcgaatct tgtccgcgat ggcggcgcgg gccgccggcg tcgtcggata
      901 ttccgcgtcc aaggccttga cggcctcccg cttgatgaag gggaggttcg tcggaatttc
      961 gcccgcggcc agggccgcgt tgacttcccg ttgcgggggg cggtagttgt ggctcggacg
     1021 attgtggcaa tcgatgcaat ccatggtccg gatttcggct ttcgcctttt cggcgtcggt
     1081 cagggcgttg tccgggttct cgtagatctt cacgcggccg tccttggaag tcgcccgaat
     1141 ccaggcgatg tcctgccgcg cccggtcccg ggcgatgtat tccaatttat tggaaatcat
     1201 catgtgccag tggatgcccg acccttcctt ggcgttcgcc gatccgcccc cgattttcac
     1261 caggagcgtg atcggatacg gcgtgttctt ctcgtcggcc agataatgcg ggttcacgtg
     1321 ttttcgttcg ccgaagaatt tctcgggcca atggcactgc aggcacgtgt cccgggcggg
     1381 gcgaaggttt ttaatgggcg tctcgatggg ccgggcgtat ttgtgaaaca tcgtggagta
     1441 aacttgatag gcgcccgtga gttttgattt cacaaaccag tccgcgcccg ggccgatgtg
     1501 gcaggccacg caagccaccc gggcgtggga ggagcgctgg taggcggtgt attccggcga
     1561 catgacggaa tggcagaggg tgccgcaaaa tttcgtggac tcggagatgt ggtaggcctt
     1621 gtaggtccca aggctcgagg cgacgagaaa caccgaggtc acggcgacgc cgatcatcag
     1681 gacccggcgg gtttgcgggt tctgaagatc gatgtggaag gaggtcccga gactcgcgga
     1741 aaggccccgc gcgcggcggc ggcgctccca gagggcgccc cccaacacca tggccaaccc
     1801 cgcgaccaaa aacccgggca aaatcatata ggtcagcatg cccagataca gcgtggtctc
     1861 ttggatgaaa aaatcgatca gaatcagcag cacgatgatc gaaaaagtcc ccacggccag
     1921 ggcggccccc gccagggaaa tccaattgta aaaatgttcc ggaaatcgac ggtcgctcat
     1981 ggaatctcct tccatttaat aaaaattctc aaacggctcc ggacgccggg gccggcggat
     2041 tcgccgccgg gaaaaccggc aacgtacgga ccgcgaggcc gaacacggcc gccactccgg
     2101 tcaacagggc gaaggacacg gacacctcca tccaatgggg ccaatagccg ggacccgtgt
     2161 agggcaccat ggccagccaa ctgacggtca ggcggttcaa caccacgccc gccaacaccg
     2221 gcaggcacgc ggccaccgtc caacggggac ggtcccggac cgcgcgacgg gacaacagga
     2281 cggccggcaa caaggcgccg ccgcccacct ccaaaaggaa aacgcccccc aaccacgccg
     2341 ggtcgccgaa gcgggcccag gcgttgcgcg ccgacacgtc caccaggcgc aacgccgtgt
     2401 agagaaacag caccatggac gccgcccggg cgaggtccgc cagcagccaa cgatcgagcg
     2461 atttttcaaa aagccgcgcc gaatagaagg acgcgacgac cgtcacgcac agcccgaccg
     2521 ccacggacga taccaaaaaa aacaccggta ggagcgtcga cgaccacagc ggatggattt
     2581 tctggggaac gatcaaaaac atcgtgccca tggaggactg gtgcaacgtc gacagaatca
     2641 cgcccaggat caccacgggg atcgcggccc ggtggagcgc ctccaccgcc cgcgtgtaac
     2701 ccaaacgctc caacaccgcg ggactgaact ccagggcgag aaccgtcaaa tagagcgtca
     2761 cgcaccaggc cacttcgaac atgatggaat gatgctgcca catcgccaga gggtgccaca
     2821 ggttgtaggg gcgcccgacc tcgatgaaca gggtcagggc gacaaagaca taaccgagga
     2881 aggccaggag caccacgggt cgaaggatcg gctcaaaccg tttgatgtgg aaaatatgca
     2941 ccgtggccgc catgacgaac ccgccgccgg acaaggccac ccccgcttga aggaacccca
     3001 cccacacccc ccaggggttg gcgtcggtca tgtgggtcat ggcccccatg ccccgggcga
     3061 accgtatcac caacaccacc gcggtcaccg ccc
//
LOCUS       DAOQLS010000188         3091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17529_length_3091_cov_3.559289, whole genome shotgun sequence.
ACCESSION   DAOQLS010000188 DAOQLS010000000
VERSION     DAOQLS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17529_length_3091_cov_3.559289"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PK362_04570"
     CDS             complement(<1..1206)
                     /locus_tag="PK362_04570"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNC74144.1"
                     /translation="MAKTKAKKVRSSRPRRAADRAVRPAAPSEYTRELVLLGRRAKEA
                     SRVLASSTAETRNRALTAMADALEKHRDDLLFKNEIDVEAGRRAGLAPALVDRLALTP
                     RRVDDMARGLREIAALPDPLGEVLSNWDRPNGLNVKKVRVPLGVVAMIYEARPNVTVD
                     ATGLCLKSGNAVILRGGKEALESNTALVNVLRAALPEAGLPAEAVQFVETTDRSVIRD
                     LVRMDRFVDLVIPRGGEEMVAAVREMATVPVLSHGKGLCALYVDKAADLAMAERLAVN
                     AKTQRPGVCNAMETLLVHRDVAERFLPGLVRRYAEAGVAVHGDPDTRRWGGAGVTAAT
                     PADYDTEFLGMEAAVKVVDSLDEAIQHINAHGSHHSDAIVTADEAAARRFLTEVDSAA
                     SSAVTMASEW"
     gene            complement(1275..2951)
                     /locus_tag="PK362_04575"
     CDS             complement(1275..2951)
                     /locus_tag="PK362_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74145.1"
                     /translation="MVVTGTASPAILAVAALPGAVALGLEIRRSVAVQRLIQGLTLYG
                     RLGTLPDGRPSMDAVKAARRLFTGRETGSASTELLVWLAGGALFAAVSLAIGLAPTFF
                     LAGFGLTLFSGRPTMSGRTLSLALVAAGAALFGLPTGAEAAGLFGIPGVAENSPLPWL
                     FGMGGIVLGVAARGVGGDAVELRARDSLKAVDGLLPVARLSEILPDQWTPAKPENQAD
                     LQAGLVQFYSAFGGAWLGFAGRAYDALSPAERSRIDVAFLNDADIRRMVSAFNALSRR
                     SPEEARGAFGVYANRVLRALARNATAKTARPDASLLRALGLFLLAQRAELFGTASTER
                     EANAVAVLTRLATLPRPSERNDMTVESSHWDLVPRPSGNTPDIAFVEVDTQDDAGLVR
                     SVISSLHNGAVILAVATDPEAAAQRLARAKETILRDHKQGVLETLRRPDHRHALDSWQ
                     DLTRRVVSRSTPGMLDEQGRYSVAGLYKAVPELSKTMGFAPGLFSGENIKYRMSILTG
                     DKTLAHWNQLGVPEIGDLVQFLILEGPKIVRMSLSITQEMEAIHVLSTNA"
BASE COUNT          521 a         1019 c         1068 g          483 t
ORIGIN      
        1 ccattcggac gccatcgtca cggccgacga ggcggccgaa tccacttcgg tcaaaaatcg
       61 gcgggcggcc gcctcgtcgg ccgtgacgat ggcgtccgaa tggtggctgc cgtgggcgtt
      121 gatgtgctgg atggcttcgt ccagggagtc gaccaccttg accgcggcct ccatccccag
      181 aaattcggtg tcgtagtcgg ccggcgtggc cgcggtcacc ccggcgccgc cccatcggcg
      241 ggtgtcggga tccccgtgga cggccacccc ggcctcggcg taccgccgga ccagtcccgg
      301 cagaaagcgc tcggccacgt cccggtggac cagcagggtc tccatggcgt tgcaaacgcc
      361 cggccgctgg gtcttggcgt tcaccgccag gcgttcggcc atggccaaat cggcggcttt
      421 gtcgacgtag agggcgcaaa ggcccttgcc gtgggaaagc acggggaccg tcgccatttc
      481 gcggacggcg gccaccattt cctcgccgcc ccgggggatg accagatcca cgaaccggtc
      541 catccggacc aggtcccgga tcaccgaacg gtcggtggtt tccacgaact ggacggcctc
      601 ggcgggcagg cccgcttcgg ggagagcggc ccgcaaaacg ttcacgaggg ccgtgttgga
      661 ttccagcgcc tctttccccc cgcgcaggat caccgcgttg ccggatttga ggcaaaggcc
      721 cgtggcgtcc accgtcacgt tcggccgggc ttcgtaaatc atcgccacca cgcccagggg
      781 cacccggacc ttcttgacgt tcaaaccgtt ggggcggtcc caattggaga ggacctcgcc
      841 cagggggtcc ggcagggccg cgatttcccg caggccccgg gccatgtcgt ccacccgacg
      901 gggggtgagc gccagccggt ccacgagggc cggggccagg cccgcccggc ggccggcctc
      961 cacgtcgatt tcgtttttaa agaggaggtc gtcccggtgc ttttccaggg cgtcggccat
     1021 ggcggtcagg gcccgattcc gggtctcggc ggtggaggaa gccagaacgc ggctggcctc
     1081 cttggcccgt cggcccagga ggaccaattc ccgggtgtat tccgaaggcg ccgcgggacg
     1141 gacggcccgg tcggcggctc ggcggggccg ggaagatcga acttttttcg ctttcgtttt
     1201 tgccatgggc ctattataga aaaaacggcc tcccccccgt gcgggaagga ggccgtttcc
     1261 gttaaaccga ttctttaggc gttggtgctc aggacgtgga tcgcttccat ttcctgggta
     1321 atcgagaggg acatccggac gattttcgga ccttccagga tcaggaactg cacgagatcc
     1381 ccgatttcgg gaacgcccaa ctggttccag tgggccaggg ttttgtcccc ggttaggatg
     1441 ctcatgcgat acttgatgtt ttcgccggag aagaggccgg gggcaaaccc catggtcttg
     1501 gagagttcgg gcacggcctt gtaaaggccc gccaccgagt accggccctg ttcatccagc
     1561 atgcccgggg tgctccggga gaccacgcgg cgggtcaggt cctgccagga atccagggcg
     1621 tggcggtggt cgggtcgacg gagcgtttcc aagacgccct gcttgtgatc ccgcaggatc
     1681 gtttccttgg cccgggcgag ccgctgggcc gcggcctcgg gatcggtcgc caccgccaag
     1741 atcacggcgc cgttgtggag ggaactgatc acgctgcgaa ccaacccggc gtcgtcctgg
     1801 gtgtcgactt cgacgaaggc gatgtcgggc gtgttcccgg agggccgggg taccagatcc
     1861 caatgggagg attccacggt catgtcgttg cgctcggagg gccgcgggag cgtggccaac
     1921 cgggtcagaa cggcgacggc gttggcttcc cgttccgtcg acgcggttcc gaacaattcg
     1981 gcccgttgag ccaaaaggaa aagtccgagg gcccgaagga gggaagcgtc gggccgggcc
     2041 gttttcgccg tcgcgttccg ggcgagggca cgaaggacgc ggttggcgta aacgccgaag
     2101 gcgcccctcg cttcctcggg actgcgccgg gagagggcgt taaaggccga caccatgcgt
     2161 cgaatgtcgg cgtcgttgag gaaggccacg tcgatgcggg acctctccgc cggcgaaagg
     2221 gcgtcgtagg cccttcccgc gaaccccaac caggcgccgc cgaaagcgga ataaaattgg
     2281 accaacccgg cctgaagatc ggcctggttt tccggtttcg ccggtgtcca ttgatccggc
     2341 aggatttccg aaagacgcgc gacgggaagg agtccatcga cggctttcaa ggagtcccgc
     2401 gcacgaagtt caacggcgtc gccccccacc ccgcgggccg cgacccccag aacgatcccg
     2461 cccatgccga acaaccaggg caggggcgaa ttttcggcca cgccggggat tccgaagagc
     2521 ccggccgctt cggcgcccgt gggcaacccg aagagggcgg cgcccgccgc caccagagcg
     2581 agggaaagcg tccggcccga catcgtgggc cgcccggaga agagcgtcaa accgaaaccg
     2641 gccaggaaga aggtcggcgc caagccgatc gccagggaga ccgcggcgaa cagcgcgccg
     2701 ccggccagcc aaacgagcag ttcggtcgaa gccgaaccgg tttcccggcc tgtgaacagc
     2761 cggcgcgcgg ctttcacggc gtccatcgag ggccggccgt cgggaagggt gcccaggcgt
     2821 ccgtaaaggg tcaaaccctg aatcaaacgt tgaacggcga cggaccgccg aatttccaga
     2881 cccaaggcca cggcgcccgg cagggcggcc acggccagaa tcgccggcga agcggtcccc
     2941 gtgacgacca cggcgaccac gcccagcgcc gaagcgatca tcgccgctgc cccgcggcag
     3001 gcgtgcgaag cctgcggttt cgatcgcgcc gtgctgtacc gcttgcgcgg caacgagttg
     3061 ctcgccgagt cgttctgggt caacggcgac c
//
LOCUS       DAOQLS010000189         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17561_length_3088_cov_4.054731, whole genome shotgun sequence.
ACCESSION   DAOQLS010000189 DAOQLS010000000
VERSION     DAOQLS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17561_length_3088_cov_4.054731"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK362_04580"
     CDS             <1..651
                     /locus_tag="PK362_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HNC74146.1"
                     /translation="AIREGRRIFDNIRLFVKYVVTTNAAEIGVLLLAPFLGLPLPLTP
                     IQLLWLNLLTDGLPGLALASESTDGHAMARPPRPPSEPLVTRALAFHIGWVSALMTGM
                     VLTAQAAFLRSGWAEWRTAVFCVMCFSQMGHALVLRSERDSFFSRRGFSNRPLLAAVG
                     LTVVLQILGVYWAPLQRLLQTAPLSPRALAGAVGASVVVFAAVELEKGLRRWRART"
     gene            648..1019
                     /locus_tag="PK362_04585"
     CDS             648..1019
                     /locus_tag="PK362_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC74147.1"
                     /translation="MKRPLPARKLSDAEARYWAARGVVYDPKIGQVLTYAGHRPAGVF
                     VLLSGGIRFRQEGGGRRRRVGDLVGPAAVGEEALGSDAPLPLTVEAGSGARVAFVPAA
                     RWRAERGEGGFSHGPSIHSHL"
     gene            991..>3088
                     /gene="ppsA"
                     /locus_tag="PK362_04590"
     CDS             991..>3088
                     /gene="ppsA"
                     /locus_tag="PK362_04590"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNC74148.1"
                     /translation="MAHQFILTFDEVGLADVPRVGGKNASLGELRRSLRAAGVRVPPG
                     FAVTADGHRAFLRFNRLEDPVSALFLGLEKGRLSLAEAGRRLRALVLAARWPEALAAE
                     IRGAYAGLGNAVDVAVRSSATAEDLPEASFAGQHESFLNVRGAAAVLDAGRRCYASLF
                     TDRAIAYRREKGFDHDKVALSLGVQRMVRADRAGAGVLFSLHTETGFPRFAVVNAVWG
                     LGENIVQGVVTPDEHLVYKPALEDAALTPIVSRRLGKKTKKMVYAPGGRTVNVPTSVR
                     ERERYVLTDEEVLILARWAVAVEKHYGKPMDMEWAKDGRTGELFMVQARPETVQSRRG
                     AGVLRTYRLTEKGRVLLRGTAVGEGIVAGPVQIIRRPQDRDRFRAGSILVTGATDPDW
                     VPVMKRAAGIVTDLGGRTSHAAIVSRELGLPAVVGTGRSTATLKEGQRITLSCAEGET
                     GVVYAGDVAHETTETRLDQLPTPRTRLLVNIASPSAALQWWRLPVHGVGLARMEFIIN
                     DVIRVHPLALVHPERVASDRERRAIARLTRGRKFPVDYFVDLLAEGIGQIAAVHHPNP
                     VIVRLSDFKTNEYAHLLGGAAFEPKEENPMLGFRGASRYYSEAYREGFALECRALKKA
                     REVLGLTNIVVMVPFCRTPEEADRVLAEMAKNGLARGREGLQVYVMCEIPSNVVLAEE
                     FADRFDGFSIGSNDLTQ"
BASE COUNT          455 a         1014 c         1083 g          536 t
ORIGIN      
        1 gccatccggg aagggcgccg aattttcgac aacatccgcc tgttcgtgaa atacgtcgtc
       61 accaccaacg ccgccgagat cggcgttctc ctgttggcgc ctttcctggg gttgcccctg
      121 cccctgaccc cgattcaact tttgtggctg aaccttctca ccgacggcct cccgggattg
      181 gccctggcgt cggagtcgac cgacggtcac gccatggccc gcccgccccg cccgccgtcg
      241 gaacccctgg tgacccgggc cctggcgttc cacatcggct gggtgagcgc gctcatgacc
      301 ggcatggtcc tgaccgccca ggccgccttt ctccggtccg gctgggcgga gtggcggacc
      361 gcggtgtttt gcgtcatgtg tttttcccag atgggccacg ccctggttct ccgttcggaa
      421 cgggattcgt ttttcagtcg ccggggtttc tccaaccgac cgctcctggc ggccgtgggt
      481 ctgaccgtgg ttcttcaaat cctcggggtc tattgggcgc ccctgcagcg actcctccag
      541 accgccccgc tgtcgccccg ggccctggcc ggcgccgtgg gggcgtcggt ggtcgtgttc
      601 gcggcggtgg aattggaaaa aggacttcgt cgatggaggg cgcggacatg aaacggcctc
      661 tgccggcccg gaaactttcc gacgccgaag cccggtattg ggcggcccgg ggcgtcgtgt
      721 acgacccgaa aatcggccag gtgttgacct acgcggggca ccgtcccgcg ggcgtgttcg
      781 tcttgttatc gggggggatt cgttttcgac aggaaggcgg aggacggcgg cggcgcgtcg
      841 gggacctggt cggtcccgcg gcggtgggcg aagaggccct gggatccgac gccccgttgc
      901 ccttgaccgt ggaggcgggg agcggcgccc gggtcgcttt tgtgcccgcg gcccggtggc
      961 gcgcggaacg cggcgaagga gggttttccc atggcccatc aattcattct cacctttgac
     1021 gaagtcggct tggcggacgt tccccgggtg ggcggcaaga acgcctccct gggcgaactt
     1081 cgacggtccc tgagggcggc gggggttcgg gtgccgccgg ggttcgcggt gacggccgac
     1141 ggacaccggg ccttcctccg attcaaccgg ctggaggacc ccgtgtcggc gctctttctg
     1201 ggtttggaaa aaggccgcct gtccctggcg gaggcggggc gccgcctccg agcgctggtg
     1261 ctcgcggccc gttggccgga agccctggcc gcggagatcc gtggggcgta cgccgggctc
     1321 gggaacgccg tggacgtggc ggtgcggtcc agcgccacgg ccgaggacct gcccgaggcc
     1381 agcttcgcgg gtcagcacga aagtttcctg aacgttcggg gcgccgccgc cgttctggac
     1441 gccgggcggc gatgttacgc cagcctcttc acggatcggg ccatcgctta ccgtcgggaa
     1501 aaagggttcg accacgacaa ggtggccctc tccctggggg tccagcgcat ggtgcgggcc
     1561 gaccgcgccg gggccggcgt gctcttctcc ctccacacgg aaaccggatt cccccggttc
     1621 gccgtcgtca acgccgtctg gggcctgggg gaaaacatcg tccagggcgt cgtcacgccg
     1681 gacgaacacc tggtctataa acccgctctg gaggacgccg cgttgacgcc catcgtcagc
     1741 cgccggctcg gcaaaaaaac aaaaaaaatg gtgtacgccc cggggggccg caccgtcaac
     1801 gtgccgacat cggttcggga acgggagcgc tacgtcctca cggacgagga agttttgatt
     1861 ttggcgcgct gggccgtggc cgtggaaaaa cattacggca aacccatgga catggaatgg
     1921 gccaaggacg ggcgaaccgg ggagcttttt atggtgcagg cccggcccga aaccgttcag
     1981 tcccgtcggg gagcgggggt gctccgcacc taccgattga ccgagaaggg ccgcgttctt
     2041 ctccgcggga cggcggtggg ggaaggcatc gtcgcggggc ccgtccagat catccggcgg
     2101 ccccaggacc gggaccgttt tcgcgccggc tccattttgg tgacgggcgc caccgatccc
     2161 gactgggtgc ccgtgatgaa gcgcgccgcg ggcatcgtga cggacctggg cggccgcacc
     2221 tcccacgcgg cgatcgtgag ccgggaactg ggactccccg ccgtggtggg cacgggccgg
     2281 tccacggcga ccctgaagga aggccagcgg atcaccctgt cctgcgccga gggggaaacc
     2341 ggcgtcgtct acgccgggga cgtggcccac gaaaccacgg aaacccgatt ggaccaactg
     2401 ccgactcccc ggacgcgcct cttggtgaac atcgcgagcc cgtcggccgc gctccagtgg
     2461 tggcgccttc ccgtccacgg cgtgggtttg gcgcggatgg agtttatcat caacgacgtg
     2521 atccgcgtcc accccctggc cctggtccat cccgagcgcg tggcctcgga ccgggagcgc
     2581 cgggccatcg cccgtctgac ccgggggcgg aaattcccgg tggattattt cgtggacctc
     2641 ctggccgagg ggatcggcca aatcgcggcc gtgcaccacc cgaaccccgt gatcgtccgg
     2701 ttgtcggatt tcaaaaccaa cgagtacgcc catttgctgg gcggcgcggc cttcgagccc
     2761 aaggaggaaa accccatgct ggggttccgg ggggcgtccc ggtattattc cgaagcctac
     2821 cgggagggtt tcgccctgga atgccgggcc ctcaaaaagg cccgggaggt cctcgggttg
     2881 accaacatcg tcgtgatggt ccctttctgc cggacgccgg aggaggcgga ccgggtgctg
     2941 gcggagatgg cgaaaaacgg tctcgcccgg gggcgggagg gcctccaagt ctacgtgatg
     3001 tgcgaaattc cctccaacgt ggtcttggcg gaggaattcg ccgaccgctt cgacggtttt
     3061 tccatcgggt ccaacgatct cacccagt
//
LOCUS       DAOQLS010000190         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17714_length_3073_cov_4.206428, whole genome shotgun sequence.
ACCESSION   DAOQLS010000190 DAOQLS010000000
VERSION     DAOQLS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17714_length_3073_cov_4.206428"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="gltX"
                     /locus_tag="PK362_04595"
     CDS             complement(<1..937)
                     /gene="gltX"
                     /locus_tag="PK362_04595"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNC74149.1"
                     /translation="MKPRVRFAPSPTGFLHIGGARTALFNWLFARHTGGTFILRIEDT
                     DETRSTQASVEAIFNGMRWLGLDWDEGPTSSDDPTQSKGVHGPYYQMNRLDHYKKYAD
                     QLVASGKAFYCFATPEEVQKAKEKAQLLKRPPKFVSPYRDLPPAQREALIQEGKPHTV
                     RFKTPQDDAPVQFDDLIRGPMKWEHSLIEDFILLKTSGVPTYNFACVIDDHLMEISHV
                     IRGDDHLSNTPRQALVYEALGWTPPIFAHLSMILGADGQRLSKRHGATAVEEYRDAGY
                     LPEATRNYLALLGWSTEDSQDLFTQDELIRKFTVER"
     gene            complement(941..1813)
                     /gene="hisG"
                     /locus_tag="PK362_04600"
     CDS             complement(941..1813)
                     /gene="hisG"
                     /locus_tag="PK362_04600"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNC74150.1"
                     /translation="MNKLKLGMPKGSLQESTIELFKKAGIRVNASERSYFPTSDDDEL
                     EIMLVRSQEMATYVEDGVFDAGLTGKDWIMESGAQVKEVCELLYAKSGFRPVRWVLCV
                     PEDSPIKSVKDLQGKRIATEVVNIVKGYLAKNKVEAKVEFSWGATEAKAGRFVDAIVE
                     LTETGSSLRANRLRIVEELLTSSTRLICNEKAWADPWKRQKIENMAMLLQGALAAEGL
                     VGIKMNIPEDKLADVTKILPALRKPTISQLTEKGWVALEVIMPEKEVKKNIPALKRAG
                     AEGIIEYPLNKIIY"
     gene            complement(1930..>3073)
                     /locus_tag="PK362_04605"
     CDS             complement(1930..>3073)
                     /locus_tag="PK362_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74151.1"
                     /translation="ILTVIPAGVSVAVLAKEGLWARVQVGAVTGYVQSAYVVGGSGRA
                     LDAVPVRFSGSDAFIFPSIKSDRTKINASREGLQRASFYRDHRHWLLIETGGRLLWAP
                     ADQVRPALNPQGVAAKTGVEALLSEEPGPTDGFRLKIDDRRTKGGGTSSALEGMGTAL
                     ALLAAYSKTPHGPAESAVPRFMALLRQRGVSITPEVQTAVETLVPSLRPEAPRQPGGV
                     AVINLLGGLKKGEFRESVLEILKTRTRVDGGPAILLVRNPQHLGAVRRQLRGLGLDPS
                     HPALRFVSLTKLTENGLADPFSQEREASPRVVLSGVLERGLSALEFRQLDEVFLHTPV
                     TDTTRWVSGGDKIKVLIVLLLAGTIVAAPLPQADDLRKALRVLQSA"
BASE COUNT          580 a          931 c          929 g          633 t
ORIGIN      
        1 accgctcgac tgtgaatttc cgaattaatt cgtcctgggt gaaaagatcc tggctgtctt
       61 ccgtggacca gcccaacaag gccaaatagt tccgggtcgc ctcgggcaaa tacccggcgt
      121 cccggtattc ctcgaccgcg gtggctccgt ggcgcttgga caggcgctgt ccgtccgccc
      181 cgaggatcat cgacaggtgg gcgaagatcg gcggggtcca acccaacgct tcgtaaacga
      241 gcgcctgccg cggcgtgttc gacaggtggt cgtcgccgcg gatcacgtgg ctgatctcca
      301 tcaagtgatc gtcaatcacg caggcgaaat tgtaggtcgg aacgcccgag gttttcaaaa
      361 ggatgaaatc ctcgatgagc gaatgctccc acttcatggg accgcggatc aaatcgtcga
      421 actgaacggg cgcgtcgtcc tggggggttt tgaaacggac ggtgtggggt ttcccctcct
      481 ggatcaacgc ctcccgttgg gcgggcggga ggtcccggta gggactcacg aatttggggg
      541 ggcgtttcaa caactgggcc ttttctttgg ctttttggac ttcttcggga gtcgcaaagc
      601 aataaaaagc cttgccggag gccacgagct ggtcggcgta ttttttgtaa tggtccaggc
      661 ggttcatttg gtaatagggg ccgtggaccc ctttgctttg agtggggtcg tcggacgagg
      721 tgggaccttc gtcccaatcc aaccccagcc aacgcatacc gttgaaaatc gcctcgaccg
      781 aggcctgggt ggaacgggtt tcgtccgtgt cttcgatgcg caatatgaac gttcccccgg
      841 tgtggcgggc gaacagccaa ttgaagagcg ccgtccgcgc gccaccgatg tggagaaaac
      901 cggtgggcga cggagcgaat cggacgcggg gtttcacggc ttaatagatg attttgttaa
      961 gcgggtattc gatgatcccc tcggcgccgg cgcgctttaa agcggggatg ttctttttca
     1021 cttctttttc cggcatgatg acttccagag ccacccaccc tttttcggtc aactgggaga
     1081 tggtcggctt tcgaagcgcc ggcaggattt tcgtgacgtc ggccaattta tcctcgggaa
     1141 tgttcatctt gatcccgacg aggccttccg cggccagagc gccttgaagc agcatggcca
     1201 tgttttcgat cttctggcgt ttccagggat cggcccaggc tttttcgttg caaatcaacc
     1261 gggtggaaga cgtgagcaat tcctcgacga tgcgaagccg gttggcccgg agggaggagc
     1321 ccgtttccgt cagctcaacg atggcgtcga caaaccggcc ggctttcgct tccgtggcgc
     1381 cccaggagaa ttccaccttc gcttccactt tattcttggc caggtagcct ttcacgatat
     1441 tgaccacttc cgtcgcgatc cgcttgccct gaagatcctt gaccgatttg atgggcgaat
     1501 cctccggaac gcacaggacc caccgaaccg gccgaaatcc cgatttcgcg tacagcaatt
     1561 cgcacacttc cttgacctgg gcgccggatt ccatgatcca gtctttcccc gtcaatccgg
     1621 cgtcgaacac cccgtcctcg acgtaggtgg ccatttcctg ggaacggacc agcatgatct
     1681 ccaattcgtc gtcgtcggaa gtcgggaaat aggaacgttc cgaggcattg acccgaatgc
     1741 ccgctttttt gaaaagttca atggtcgatt cctgcaggct gcccttgggc atgcccagtt
     1801 ttaatttatt catggcggct cccgaaatgt ggtgtgggcc ctatgttagc aaatatcgcc
     1861 tcctcgaacg tcatcgggcc ccgctctcgg ggaggacggc ggggcccgcg atggggggtt
     1921 caaaccgcgc taagccgatt ggagaacccg gagggccttc cgcaggtcgt cggcttgggg
     1981 caagggcgcg gcgacgatcg ttccggccag caacagaacg atcagaacct tgattttatc
     2041 gccgcccgaa acccaacggg tcgtgtcggt caccggcgtg tgcaggaaga cttcgtccaa
     2101 ttggcggaat tccaaggccg acaagccgcg ctccaacacg ccggacaaaa ccacccgggg
     2161 cgaggcttcc cgttcctggc tgaagggatc cgccaagccg ttttccgtca acttggtcag
     2221 cgacacaaac cgcagagcgg gatgggacgg atcgagcccg agaccgcgca gctgacgccg
     2281 aacggccccc aggtgttggg gattgcgcac cagaaggatc gcgggccccc cgtcgacccg
     2341 ggttcgtgtt ttgagtattt cgagaaccga ttcccggaat tcgccttttt tcaggccgcc
     2401 caacaaattg atgacggcca ccccccccgg ttgccggggg gcttccggac gaagcgaggg
     2461 caccagggtt tccacggcgg tttgaacctc gggcgtgatc gaaacgcccc gttgacgcaa
     2521 cagcgccatg aatcgcggaa ccgccgattc cgccggaccg tgcggcgttt tcgaataggc
     2581 cgccaggagc gccaaggcgg ttcccatccc ctccaaggcc gacgacgtcc cccctccttt
     2641 cgtcctccgg tcgtcgattt tcaatcggaa tccatcggtg ggtcccggct cctcggacag
     2701 aagcgcttcc acgcccgtct tggccgccac cccctgcggg ttgagcgccg gacgaacttg
     2761 gtccgccggc gcccagagca accggcctcc ggtttcgatc aacagccaat ggcggtggtc
     2821 ccggtaaaag gaggcgcgtt ggaggccctc ccgggaggcg ttgattttcg ttcgatcgga
     2881 tttgatcgac ggaaagataa aggcgtcgga cccgctgaat cgaaccggga ccgcgtccag
     2941 ggcgcggccc gaaccgccga ccacgtaggc cgattgaaca tacccggtca ccgcgccgac
     3001 ctggacccgg gcccagagtc cttccttcgc caatacggcg acgctgaccc cagcggggat
     3061 aacggtgagg att
//
LOCUS       DAOQLS010000191         3057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_17870_length_3057_cov_4.693205, whole genome shotgun sequence.
ACCESSION   DAOQLS010000191 DAOQLS010000000
VERSION     DAOQLS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17870_length_3057_cov_4.693205"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK362_04610"
     CDS             <1..415
                     /locus_tag="PK362_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNC74152.1"
                     /translation="ERRVVLGALPFSYLLFVVGASLGWFVIVPVGLKLLTGFATADLR
                     PTLSVTAVLEFALWTSGGLGLLFQLPVAIAALASWGFVRSATLGRYRRHAAVAILILS
                     AVVTPGPDLFSQLALALPTYALFEISIAVARVLEP"
     gene            485..802
                     /locus_tag="PK362_04615"
     CDS             485..802
                     /locus_tag="PK362_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020199041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNC74153.1"
                     /translation="MAAIDPDFQKNRTKLADHAGHAVWGPEEKPAKLGIHGTWVAVDF
                     DLCIADGACLDACPENVFTWLDTPGHPASAKKADPSKEDSCIFCMACESVCPVVAIKI
                     TPK"
     gene            1158..1952
                     /gene="rpsB"
                     /locus_tag="PK362_04620"
     CDS             1158..1952
                     /gene="rpsB"
                     /locus_tag="PK362_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNC74154.1"
                     /translation="MASISMKALLEAGVHFGHQTRRWNPKMSKYIFGSRNNIHIIDLQ
                     KTVKELKKALTYVRDLAADGKTLLFVGTKKQAREAISQEAARCGMPFIRDRWLGGTLT
                     NFETVRRSAKRLQELERMKQSGVFGSLSKKETMRREKDIKRLAKSLDGIKNMDSLPHA
                     LFIVDPVQEITAVTESRRMEIPIVSICDTNCDPDLIDWPMPGNDDAIRSVRLFCKLVA
                     DSVLEGREMAKRKDPAAADASAPEGEIALTDSAADAPVVPVETTNA"
     gene            1967..2830
                     /gene="tsf"
                     /locus_tag="PK362_04625"
     CDS             1967..2830
                     /gene="tsf"
                     /locus_tag="PK362_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNC74155.1"
                     /translation="MPVVTSDLVAQLRAKTGAGLMDCKRALTSTEGDFEKAIQILREQ
                     GAASAAKRSGRATAAGLVQATVAAGGKTAVLLELNCETDFVARTDDFKGLLNDLLQKA
                     AAATPAWSSAADAPQERVKELAAKLGENIVLKRFARFDRPAAGLFASYIHPSESVKVG
                     ALIELAAASDKGASAPEAAELGKVLAMQAAANGARWVNPQDIPADLIEKEKAIGREKA
                     KNEGKPEKIWDKIAEGNLKKFFQDFCLLEQADNLNPKSTLRQRVEDVSKKLGEPVTVK
                     RFARFKVGEED"
     gene            2841..>3057
                     /gene="pyrH"
                     /locus_tag="PK362_04630"
     CDS             2841..>3057
                     /gene="pyrH"
                     /locus_tag="PK362_04630"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNC74156.1"
                     /translation="MPTPQRVRRVVLKLSGEALLGRAASGIDVDALFGIAQEIKSAQK
                     AHHQIAVVVGGGNIWRGGRGQGKELDRV"
BASE COUNT          618 a          992 c          903 g          544 t
ORIGIN      
        1 cgagcgccgg gtcgtgttgg gcgccctccc gttctcttac ctcctgttcg tggtgggcgc
       61 gtccttgggc tggttcgtca tcgtgcccgt gggtctcaag ttgttgacgg ggttcgcgac
      121 ggccgacctc cggcccaccc tgtcggtgac cgcggtgctg gagttcgctc tgtggacatc
      181 gggcgggctg ggcctcctct ttcaacttcc cgtggcgatc gccgccctgg cgtcatgggg
      241 ctttgttcgt tccgccaccc tcggccggta tcggcggcac gccgccgtcg ccattttgat
      301 cctctcggcg gtcgtgacgc ccgggcccga ccttttttcc cagttggccc tcgcgctccc
      361 gacttacgcc ctcttcgaaa tttccatcgc cgtggcgcgc gtattggaac cttgacaccc
      421 tccgggccgt gttatacact tttgctttcc attaccattg ttgatcaccg aagggagacg
      481 caccatggca gccatcgatc cggatttcca gaaaaaccga accaaactcg ccgaccacgc
      541 cggtcacgcc gtttggggac ccgaagaaaa acccgcgaaa ttgggcattc acggcacctg
      601 ggtcgccgtg gatttcgatt tgtgcatcgc cgacggcgcc tgtctggacg cgtgccccga
      661 aaacgtgttc acctggttgg acaccccggg ccatcccgcc tcggccaaga aagccgatcc
      721 ctcgaaagaa gactcctgca ttttctgcat ggcctgcgaa agcgtttgcc cggtggtggc
      781 cattaagatc accccgaaat aaaccgctct tgaacccgaa aacccgcccc ggccccgacg
      841 gccggggcgg gttttttgtt tgccttcgcg atttggaatt gatataattg gatcgctgtg
      901 aaccgcagga gcggcgatgt cggccgctca aattcacaca tccccggatg gccgtcgagg
      961 tcccagagtc catgggtcgg cgggcgagcg aagggggtgg aggagcgggg ggcgattgac
     1021 cccgcaaaaa ccagacacag gtcatcggaa acgccgcttc ggcggcgaaa ttccacgctg
     1081 tgaacgggcg gtcgcgcatt cggcggccgt ttttttttcg gcggaccggt gtcacactca
     1141 aggaggaatt cgcaccaatg gcatccattt ccatgaaagc gttgctcgag gccggcgttc
     1201 atttcggcca ccaaacccgt cgctggaacc cgaagatgtc gaaatacata ttcggcagcc
     1261 gcaacaacat ccacatcatc gacctgcaaa aaacggtcaa ggaactcaaa aaggccctga
     1321 cgtacgtccg agatctggcg gccgacggga aaaccctcct ctttgtcgga accaaaaagc
     1381 aggcccggga agccatttcc caggaagccg cccgttgcgg catgcctttc attcgggacc
     1441 gatggttggg cggcacgctg acgaactttg aaaccgtccg ccggtcggcc aagcgccttc
     1501 aggaattgga acggatgaaa cagagcggcg tgttcggttc gctctccaag aaggaaacca
     1561 tgcgccggga gaaagacatc aagcgcctcg ccaaatccct ggacggcatc aaaaacatgg
     1621 acagtttgcc ccacgcgctc ttcatcgtcg acccggttca ggaaatcacc gcggtgacgg
     1681 aatcgcgccg gatggaaatt cccatcgtct ccatctgcga cacgaactgc gaccccgatc
     1741 tcatcgattg gcccatgccg ggcaacgacg acgccatccg ctcggtccgc ctcttctgca
     1801 agttggtggc cgacagcgtt ctggaaggcc gcgaaatggc caagcgcaag gacccggccg
     1861 ccgcggacgc ctcggccccc gagggggaaa tcgcgctcac ggacagcgcc gcggacgcgc
     1921 cggtcgtccc ggttgaaacc accaacgcct aatttcggag accgccatgc ccgtcgtcac
     1981 cagtgatctc gttgcacaac tccgcgccaa aaccggcgcg ggcctcatgg attgcaaacg
     2041 cgccctcacc agcaccgaag gcgatttcga gaaggccata caaatccttc gtgagcaagg
     2101 cgcggcgtcc gccgccaagc ggtccggccg cgccaccgcg gcgggtctcg ttcaagccac
     2161 cgttgcggcc ggcgggaaaa ccgccgtgct gttggaactc aattgcgaga cggactttgt
     2221 cgcccgcacg gacgatttca aaggcctgtt gaacgacctt ctgcaaaagg ccgccgcggc
     2281 cacgccggcc tggtcgtccg ccgcggacgc cccccaggaa cgcgtgaagg aattggccgc
     2341 caagctcggc gaaaacatcg tgttgaagcg tttcgcccgg ttcgaccgac cggccgccgg
     2401 cttgtttgcg tcctacatcc acccgagcga aagcgtcaaa gtcggcgccc tgatcgagtt
     2461 ggccgcggcg tccgacaaag gcgcctccgc ccccgaggcc gccgaattgg gcaaggtgtt
     2521 ggccatgcag gccgccgcca acggcgcccg atgggtcaat ccccaggaca ttcccgccga
     2581 cctcatcgaa aaagagaaag ccatcggacg ggaaaaagcc aagaacgaag gcaaacccga
     2641 aaaaatctgg gacaaaattg ccgaaggcaa tttaaaaaag ttcttccagg atttttgcct
     2701 cctggagcaa gcggacaacc tgaaccccaa atccaccctg cgacagcggg tggaggacgt
     2761 ttccaaaaaa ctgggcgagc cggtgacggt caagcgtttc gcccgcttta aagtcggcga
     2821 agaggactaa ccgccgcccc atgcccaccc cccaacgcgt tcggcgggtc gtgctcaagc
     2881 tgtccgggga agcccttctc ggacgcgcgg cctcgggcat tgatgtcgac gcgctttttg
     2941 ggatcgccca ggaaatcaaa tccgcccaaa aggcccacca tcagatcgcg gtggtggtcg
     3001 gcggcgggaa catctggcgc ggagggcgcg ggcagggcaa ggaactggat cgcgtca
//
LOCUS       DAOQLS010000192         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_18009_length_3046_cov_2.862253, whole genome shotgun sequence.
ACCESSION   DAOQLS010000192 DAOQLS010000000
VERSION     DAOQLS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18009_length_3046_cov_2.862253"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..456)
                     /locus_tag="PK362_04635"
     CDS             complement(139..456)
                     /locus_tag="PK362_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74157.1"
                     /translation="MPRIGRLHFLLPALLLLSGMTRAGRREENKTLERWTQKLGLRPD
                     QQTAVQALLAIPDGAEPRSLDFKTLESLDKILTPDQRVKFANLREVKKAKKCRFDRCC
                     PPE"
     gene            complement(462..1385)
                     /locus_tag="PK362_04640"
     CDS             complement(462..1385)
                     /locus_tag="PK362_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC74158.1"
                     /translation="MDLQPLRAFLAVARGGGYAKAAEALHRTQPAVTAAVQSLERELR
                     RPLFERRGRRAVLSPAGRALLAEAGPLLDQWEKVPDRLAERLEGGFRGELRIGAGESA
                     TLYVLPALLGDFQRKHPEVRLVIHHQRADETLRMLRSGELDFGVRPLTTVPEWAHYRP
                     FRSYKRLAVCAQKHPFGRSRGPVTLRQMAEHPLLVPGSQSITRILMEGALSRAGLAYR
                     VGLEAGGWEAIKTYAAAGLGVALVPDVCLVPADRRRLAVRDASALFGEDAQGIVTRRG
                     ALLSNSARAVIRALDPHFPFNEDPRGEKLLK"
     gene            1492..1737
                     /locus_tag="PK362_04645"
     CDS             1492..1737
                     /locus_tag="PK362_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74159.1"
                     /translation="MQTFAPRLRRYDSHDSAKDGALYCRCGTVAPAEITEYYRHYHVL
                     GCTRCRTPLALVDHPLSQEFEIFDGYPLSGVNNPLAA"
     gene            1797..2624
                     /locus_tag="PK362_04650"
     CDS             1797..2624
                     /locus_tag="PK362_04650"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNC74160.1"
                     /translation="MRTGAIHATGWGAVVLAAAAWAAADGPAPLAVPAHRTMENRAFG
                     VGERLQYQVHFSGVSGGTVVQEVVSIESLAGRPAYRVVSEARTNKTFDLFHRTRDRNE
                     SWIDVQNLSSLKYVESIEEGSYRKRAETVVDASAGRLQHTYKTTKHEGQTSVDVPPFP
                     QDAVSIVYFLRVRPLRVGDVFDLPTYSGGEVYRSTVRVGEMEKVKVPAGVFQCYHVVP
                     SLDGDAKQKNQIEVWLSTGPERWPVLLKSRLTFGSFTARLTDRVPGRIEPPPASVAQ"
     gene            complement(2618..>3046)
                     /locus_tag="PK362_04655"
     CDS             complement(2618..>3046)
                     /locus_tag="PK362_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC74161.1"
                     /translation="LFHPAEHVTDAVTHVNTLTHVIIRSVMFTWVLAFGVALFYSHLI
                     AGPIYRFGHVLGRMKQGDLTLHVRLRRFDEFQDLAKDFDEVLTILRQKARGDREFVYD
                     RLSLIEKRLEEMKNPADPEKINGVLTQIREIKEKPSSFLY"
BASE COUNT          595 a          914 c          956 g          581 t
ORIGIN      
        1 ggaattcacc gtttttaaag ccacgattgg ctagaaaatg aaattacatg acaaattctt
       61 gccttttatt ggcaataagt caatttcggg gggggcgctc gacgggttta tcgacgaaaa
      121 aacgccaaaa aatggaggct attcgggggg acaacagcgg tcaaaacggc atttcttagc
      181 tttcttgacc tcccggaggt tggcaaattt gacccgttgg tccggcgtta aaattttatc
      241 gagggattcg agggttttga agtccaggga acggggctcg gccccgtcgg gaatggccag
      301 aagggcttga acggcggttt gttggtccgg gcggagcccc aatttttggg tccaccgctc
      361 aagggttttg ttctcttcgc gccggccggc acgggtcatc ccggagagga gaaggagggc
      421 cgggaggagg aagtggaggc ggccgattcg gggcattgac gttattttaa cagtttctcc
      481 cccctcgggt cttcgttaaa aggaaagtgg ggatccaggg cccgaataac ggcccgggcg
      541 gaattggaaa ggagggcccc gcgccgggtg acgatgccct gggcgtcctc tccgaagagg
      601 gcgctggcgt cccggacggc cagccggcgg cgatcggccg ggaccaggca aacatcgggg
      661 accagggcca cccccagtcc cgccgccgcg tacgttttga tggcctccca cccccccgcc
      721 tccagcccca cgcggtaggc caagccggcc cggctcaggg ccccttccat gagaatccgg
      781 gtaatggatt gggagcccgg caccagaagg gggtgttcgg ccatttgccg tagcgtcacg
      841 gggccccggg agcggccaaa ggggtgtttc tgggcgcaca ccgcaagtcg tttataggac
      901 cggaacgggc gatagtgcgc ccactccggc accgtggtca ggggacgaac gccgaaatcc
      961 agctcccccg accggagcat gcgaagggtt tcgtcggccc gttgatggtg aatgaccagg
     1021 cgaacttcgg ggtgtttccg ctggaagtcg cccaggaggg ccggaagaac atagagagtg
     1081 gcggattccc cggcgccgat gcgcagttcc ccccgaaagc ccccctccag gcgttcggcc
     1141 aggcgatccg gcactttttc ccattgatcc aacaacggcc cggcctcggc cagcagggcc
     1201 cgtccggccg ggctgaggac ggcccggcgg ccccgtcgct cgaacagcgg gcgtcgaagc
     1261 tcccgctcca gggattgaac ggccgcggtc acggccggtt gggtccggtg gagggcctcg
     1321 gcggctttgg cgtagccccc gccccgggcc acggccagga acgcgcgcaa cggttgcagg
     1381 tccatgaaca tatattatca aattttatgt taattataaa aacaatcaat ttgatttata
     1441 ttaaatccgg cggtaaagtg ggcgggacga accaaatcaa ggaggaacac catgcaaacg
     1501 tttgccccgc ggcttcgccg ctacgactcc cacgattccg ccaaagacgg cgccctctat
     1561 tgccgttgcg gcaccgtggc cccggccgaa atcacggagt attaccgcca ttaccacgtt
     1621 ttgggatgca cccggtgccg aacgcccctc gccctggtcg atcatccgct gtcccaggaa
     1681 ttcgagattt tcgacgggta tccgctttcg ggcgtcaaca atcctttggc ggcgtaaccc
     1741 cccccggggg ggagcggttt gacttttggg ttcaatcccc ctatactccc cggcatatgc
     1801 gaacgggggc cattcacgcg acggggtggg gagcggtcgt tctggcggcc gcggcctggg
     1861 cggccgccga tggcccggcc cccctcgccg ttccggccca tcggacgatg gaaaatcgcg
     1921 ccttcggggt gggcgagcgg cttcaatatc aggtccattt cagcggggtg tccggcggga
     1981 ccgttgtgca ggaggtggtt tccatcgaat ccctggcggg gcggcccgcc taccgggtgg
     2041 tctcggaggc ccggacgaac aagaccttcg atctttttca ccgcacccgg gatcgcaacg
     2101 aatcctggat cgacgtccaa aacctctcgt ccctgaaata cgttgaatcc atcgaggaag
     2161 ggtcctaccg aaagcgggcc gaaacggtgg tcgacgcgtc cgcgggccgc ctccaacaca
     2221 cgtacaaaac gacgaaacac gaagggcaaa cgtcggtgga cgttcccccc tttcctcagg
     2281 acgccgtctc gatcgtctat ttcctccggg ttcgcccgtt gcgggtcggg gacgtgttcg
     2341 acctgcccac ctattccggc ggggaggtct accggtcaac ggtgcgcgtg ggggagatgg
     2401 aaaaggtcaa agtaccggcg ggcgtatttc agtgctacca cgtggtcccg tccctggacg
     2461 gggacgcgaa acaaaaaaat cagattgagg tttggttgtc gacgggcccg gagcgttggc
     2521 ccgttctttt gaaatcgcgc ctaaccttcg ggtccttcac ggctcgttta acggaccgcg
     2581 tcccgggaag gatcgagccg ccgcccgctt ccgtcgctca gtagaggaag gacgagggtt
     2641 tttctttgat ttcgcgaatt tgagtcaaca ccccgttgat cttttccgga tcggcggggt
     2701 ttttcatttc ctccagccgc ttttcgatca acgacaaacg atcgtacacg aattcccggt
     2761 cgccccgggc cttttgccga agaatcgtca acacttcgtc gaaatccttc gccaggtcct
     2821 gaaactcgtc aaaacgccgc aaccgcacgt gcagggtcaa atccccctgt ttcatccggc
     2881 cgagcacgtg gccgaaccga taaatgggcc ccgcgatcag gtgggagtaa aacaacgcca
     2941 cgccgaaagc caaaacccag gtgaacatga cgctgcgaat aatcacgtgg gtcagggtgt
     3001 tgacgtgcgt gacggcgtcg gtcacgtgct cggccggatg aaacag
//
LOCUS       DAOQLS010000193         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_18313_length_3019_cov_4.266194, whole genome shotgun sequence.
ACCESSION   DAOQLS010000193 DAOQLS010000000
VERSION     DAOQLS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18313_length_3019_cov_4.266194"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK362_04660"
     CDS             <1..761
                     /locus_tag="PK362_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC74162.1"
                     /translation="RLSVSVDNARETMSQAASVSSSAEQVSKSNQTVATGVEELAASI
                     REVAKSAHEAAQVASMGVKIAEATNATVARLGESSGEIGKVVDVINRIAEQTNLLALN
                     ATIEAARAGDAGKGFAVVANEVKELAKETAKATDDIRERISSIQTETQSAVRAIVEIS
                     NIIKRISSFQGTIAGAVEEQSATTSEIGHSAAEAARGSVQIVQSITTVAEMARGTVTQ
                     AETLRESTTDLRAKMAAALERIVGRWRRARGSEV"
     gene            783..1619
                     /locus_tag="PK362_04665"
     CDS             783..1619
                     /locus_tag="PK362_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNC74163.1"
                     /translation="MTPDEFQWVQRIAKEDAGLVLDAGRAYLAEIRLADLARREGPGT
                     VAELIGRARREGDGSLRRRVVESLLVHETRFFRDEASFDLFRDWVLPELLRRPAQPAV
                     AVWSAGCSSGQEPYSLAMILGERLPAGVKKRILATDLSEAALARARAGVYHELEIHRG
                     LTPERRERYFTAVGHGDWRINDALRESVEFRPLNLVKEWPAVLPMDVVFLRNVMIYFD
                     QETKKALLARVRRVLRPGGFLFLGGTETALMTDPQYRLIEKNKSWCYQTPGPTTDSGG
                     MG"
     gene            1616..1999
                     /locus_tag="PK362_04670"
     CDS             1616..1999
                     /locus_tag="PK362_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74164.1"
                     /translation="MSLNVLIVDDSGVMRKMIRQALDRSGLSVGGVYEAANGREALDL
                     LERERVDVALLDLHMPVMTGEELYESLRTSARHADLPVVFVSSESSAGRIQHLMEKGA
                     GFVHKPWSAEDLRAQIESVTEARRG"
     gene            2022..2429
                     /locus_tag="PK362_04675"
     CDS             2022..2429
                     /locus_tag="PK362_04675"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNC74165.1"
                     /translation="MAAETVESLAGLLPCPRESPAQSAAPWEAAFRVTFEGAGRGEMR
                     LTFFGGAGAAVAAGMIGRREGADAADALGELANIVCGNFLPFVYGAEARCRLSRPTPV
                     QPGDPPAGAPGAAARLPLGSGRLDIEFREGGRP"
     gene            2502..2720
                     /locus_tag="PK362_04680"
                     /pseudo
     CDS             2502..2720
                     /locus_tag="PK362_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2940..3005)
                     /locus_tag="PK362_04685"
                     /pseudo
     CDS             complement(2940..3005)
                     /locus_tag="PK362_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          499 a          938 c         1090 g          492 t
ORIGIN      
        1 accgtttgtc ggtgtccgtg gacaacgccc gggaaaccat gtcccaggcg gccagcgttt
       61 cctcgtcggc ggagcaggtg tccaaaagca accaaaccgt ggccacgggc gtcgaggagt
      121 tggcggccag cattcgggag gtggccaaga gcgcccacga agcggcccag gtcgcctcca
      181 tgggcgtcaa aatcgccgaa gccaccaacg ccacggtggc ccgtctcggg gaatcgagcg
      241 gggaaatcgg aaaggtcgtg gacgtcatca accgcatcgc ggagcagacc aatttgctgg
      301 ctttgaacgc caccatcgag gccgcccggg ccggcgacgc gggaaaaggg ttcgccgtcg
      361 tggccaacga ggtgaaggag ctggccaagg aaacggccaa ggccaccgac gacattcggg
      421 agcgcatctc ctccattcaa acggagaccc aaagcgccgt ccgcgccatc gtggaaatct
      481 ccaacatcat caaacgcatc agcagctttc agggaaccat cgccggggcc gtggaggagc
      541 agtcggccac gaccagcgaa atcggacact ccgcggccga agcggcccgg ggcagcgttc
      601 aaatcgtcca gagcatcacg accgtggccg agatggcgcg gggaacggtg acccaggcgg
      661 aaaccctccg ggagtccacg acggatttga gggcaaaaat ggccgccgcc ctggagcgca
      721 tcgtgggccg ctggcggcgg gcccggggct ccgaggtttg agggcgggga aaggaccacg
      781 gggtgacgcc ggacgaattt cagtgggttc agcggatcgc gaaggaagac gccgggctgg
      841 tgctcgacgc cggacgggcg tacctcgccg aaatccgttt ggccgacctg gcccgccggg
      901 agggcccggg gacggtcgcc gaactgatcg gccgggcgcg gcgggaaggc gatggaagcc
      961 ttcggcggcg ggtcgtggaa tcgttgctgg tgcacgaaac gcggtttttc cgggacgaag
     1021 cctccttcga tcttttccgc gattgggtcc ttcccgagtt gctccggcgg cccgcgcaac
     1081 ccgcggtggc ggtgtggtcc gccggttgtt ccagcggcca ggaaccctat tctctggcga
     1141 tgatattggg cgaacgactg cccgcgggcg ttaaaaagag gattctggcg acggatttgt
     1201 cggaggcggc cctggcccgg gcccgcgcgg gggtgtacca cgaattggaa attcaccggg
     1261 ggctgacccc ggagcgccgc gagcgttact tcacggcggt gggccacggc gattggcgga
     1321 tcaacgacgc tctgcgggag tccgtggaat ttcgcccttt gaatttggtc aaggagtggc
     1381 cggcggtgct ccccatggac gtggtgttct tgcgcaacgt catgatttac ttcgatcaag
     1441 aaaccaagaa ggctctgctg gcgcgggtgc gccgggtgct gcgtcccggc gggtttttgt
     1501 ttctgggggg gacggaaacg gccctcatga ccgatcccca gtaccgcctc atcgaaaaaa
     1561 acaagtcctg gtgctaccaa acaccgggac cgacgaccga ctccggagga atgggatgag
     1621 ccttaacgtg ctgattgtcg acgacagcgg ggtcatgcgc aagatgattc gccaggcgct
     1681 cgatcgctcg gggttgtccg tggggggcgt ttacgaggcg gccaatgggc gggaggccct
     1741 ggatttgctg gagcgggagc gggtggacgt ggccctgctg gacctgcaca tgcccgtgat
     1801 gaccggggag gagttgtacg aatccctgcg gacctccgcc cgccacgcgg atcttccggt
     1861 cgtgttcgtg tccagcgagt ccagcgccgg gcgcatccag cacttgatgg aaaaaggcgc
     1921 ggggttcgtg cacaagcctt ggtccgctga agatttacgg gcgcaaatcg aatccgtcac
     1981 cgaggcgcgc cgtgggtgaa agggacgacg ctcttttccg cgtggcggcg gaaaccgtcg
     2041 agtccctcgc cgggcttttg ccctgtcccc gggaatcgcc cgcgcaatcg gcggccccct
     2101 gggaggccgc gttccgggtg acgtttgaag gcgcgggccg gggcgaaatg cggctgacgt
     2161 tctttggggg ggcgggcgcg gccgtcgccg ccggcatgat cgggcgccgg gaaggcgccg
     2221 acgccgccga cgccctgggg gagctggcca acattgtttg cggtaatttt ttgcccttcg
     2281 tctatggcgc cgaggcccga tgccgcctgt cccgaccgac gcccgtccag cccggggatc
     2341 cgcccgccgg cgcgcccgga gcggccgccc gccttcccct gggttcgggg cgcctcgaca
     2401 tcgaatttcg ggagggcggg cgtccctgag ggggcccgcc ggtcgtgagc cgccttcgcg
     2461 tggtcgcggt cgacgactcc gccgtgcttc gtcggctcct ggtggaaacg ctggaggccg
     2521 atccggaggt gtcggtggtc gccgcggccc ccgacggcgc gaccgggttg tcgtcggtga
     2581 tcgcccagaa acccgacgtc gtgattttgg acattgaaat gcccgggatg aacgggctgg
     2641 aggcgctcag ggaattgcgc cgcctttttc cccggctgcc catcgtcatg tattcccatt
     2701 tgaccgaccg tggcagtcga tcgaccttgg aagccctgtg cctgggggcc acggattacg
     2761 tgcccaagcc gccagcgggc caagggctgg aggacgcccg gcgccatgtt cgaacggtgc
     2821 ttctgccccg gctcaaggcg cttcacgagg aggaacaccg gcggctgcgc gaggcgcccg
     2881 tcgcccgcgt cgcagaggaa gatgttgctc ggcttgatgt cgcggtgaac gatgccgcgc
     2941 tcgtgcgcgc gcgagagggc gcgcgccacc tgcgagacga cctcggcgac cgcggccggg
     3001 tccatcgccc cgtgcgcgg
//
LOCUS       DAOQLS010000194         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_18499_length_3002_cov_4.686800, whole genome shotgun sequence.
ACCESSION   DAOQLS010000194 DAOQLS010000000
VERSION     DAOQLS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18499_length_3002_cov_4.686800"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PK362_04690"
     CDS             complement(<1..553)
                     /locus_tag="PK362_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC74166.1"
                     /translation="MRLDRVARLARKEWAEQANGTLWFLTQGLLLFLLGWAQTSSLFL
                     NGQADLFEYFDVLPFLFAVFMPAFTMRLFAEEYRLGTFDLLAAQPVSDAELVAGKFVA
                     ALGGLGATLLGGHALIVPLRYLGRPDVRALEVAFLGAALVGAFFLALGLWASTMTRSQ
                     AVALLAGFLLCFTFLGLGPAGRYL"
     gene            complement(543..1490)
                     /locus_tag="PK362_04695"
     CDS             complement(543..1490)
                     /locus_tag="PK362_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC74167.1"
                     /translation="MISAPAIVLDRICRDFSGIPAVADLSFEVNPGEVVGLLGPNGAG
                     KTTTLRVLAGALAPSRGRARIGGADVFDSPPDRRALVGYLAEGNPLYETLDVWQLFSF
                     LAKLRGLSNGQTRDRFRLLTAECGLSSVVDRPVHQLSKGFRQRLGLALALVNDPPVLL
                     LDEPGTALDPHQRKDLWELIGRIRRDKAVLLSTHILPEAESLCDRLIVLHRGRLVAQG
                     TPSHVAATFSAPAALRIRLRGPRDAAANLSVLEGVTSVETLPDSDDGDVQLRIHFTAD
                     PRDAVARTAVAHGWVLRDIQLERPSLDDVFRRLAPPHAP"
     gene            1608..1889
                     /locus_tag="PK362_04700"
     CDS             1608..1889
                     /locus_tag="PK362_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74168.1"
                     /translation="MIRPVWWTAALLAVVGATACKKKTEIKIGAPVPVSMFKEAPVLP
                     ARAVINNSPTTPPAPPPAHMEEPPPLPMPDKPQERSGDKLRRAPPDPNS"
     gene            1973..>3002
                     /locus_tag="PK362_04705"
     CDS             1973..>3002
                     /locus_tag="PK362_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNC74169.1"
                     /translation="MRKTRRKLPIGVTLTELMIAVAVISVGALGLLGAFGGIARSVQS
                     SKAKTLATNLCQEKTQILKQKNYYRVLVTTAPDYNTDFPSPIPYDPSTGYFPPETIVE
                     GGITFTRLTFVEVAQEVGTDIVTLAPTSSDTGLKLITVTALWTTGNEPKELHVRTLVT
                     NPDATVQNSVLKGFVQRAGPIAIPNAAVSVSENSGWVDTTDGTGLYTINLFPGSYNIL
                     VEAPGYFPQLVAVTVPANATVNQNITLAPMTTQTVQGYAWIRDHLVISQVVASSESAA
                     APGFYQEYVEVFNPTTSTVQMKGRFGLKFQRRVAQDALPRVIDIDYDYKTPVSTNIAP
                     GGYYLFANT"
BASE COUNT          639 a          959 c          930 g          474 t
ORIGIN      
        1 cgagataacg tccggcgggc cccaacccca ggaacgtgaa acacaagaga aaacccgcca
       61 aaagcgcgac ggcttggctg cgggtcatgg tcgaggccca cagtcccagc gccaaaaaaa
      121 aggcccccac caaggcggcg cccaaaaaag cgacttcgag ggcccgcacg tccggtcgac
      181 ccaggtaccg caacgggacg atgagcgcgt ggcctcccag gagggtcgcc cccaagccgc
      241 ccagggccgc cacgaatttc ccggcaacca attcggcgtc gctgacgggt tgcgcggcca
      301 gaagatcgaa ggtccccagc cggtactctt cggcgaacag gcgcatggtg aaggcgggca
      361 tgaaaacggc gaacaaaaat gggaggacgt cgaagtactc gaacagatcg gcctggccgt
      421 tcaggaatag ggaagaggtc tgggcccagc ccaggagaaa caacagcagg ccttgggtca
      481 ggaaccacaa cgttccgttg gcctgttccg cccattcctt ccgggccaac cgggcgacgc
      541 ggtcaaggcg catggggggg ggccaaccgg cgaaaaacgt cgtcgaggga cggccgctcc
      601 aattgaatgt cgcgcagaac ccaaccgtgg gccaccgcgg tccgggccac ggcgtcccgg
      661 gggtcggcgg tgaaatggat ccgaagttgg acgtcgccgt cgtccgagtc gggaagcgtt
      721 tccaccgagg tcaccccttc caagacggac aggttggccg ccgcgtcccg gggcccccgg
      781 agccggattc tcagcgccgc gggggcggaa aaagtcgcgg cgacgtggga aggcgttccc
      841 tgggccacga ggcgcccgcg gtggaggaca atcaaccggt cgcacaggga ttccgcttcc
      901 ggcaggatgt gggtggaaag aagcacggcc ttgtcccgtc ggatccgccc gatcagttcc
      961 caaaggtcct tgcgttggtg ggggtcgagg gcggttcccg gttcgtccaa cagcaacacc
     1021 ggggggtcgt tgaccaaagc cagggcaagg cccagccgct gccggaatcc tttggaaagt
     1081 tgatgaacgg gccgatcgac caccgaggaa aggccgcatt cggcggtcag aaggcgaaac
     1141 cggtcccggg tttgcccgtt ggacaggccg cgaagtttgg ccagaaagga aaagagttgc
     1201 cagacgtcga gagtttcgta gaggggattg ccttcggcca aatacccgac aagcgcccgc
     1261 cggtcggggg gcgaatcgaa aacgtccgcg ccgccgatcc gcgcgcgccc ccgggagggg
     1321 gccagcgccc ccgccaggac gcgcagggtc gttgtcttcc ccgcgccgtt gggacccagg
     1381 agcccgacca cctccccggg gtttacttca aaggataaat ccgccacggc cggaatcccg
     1441 gaaaaatcgc ggcaaattcg gtcgagaaca atggcggggg cggaaatcac gggcgctttg
     1501 gattataaca attccaaact tcaggcccgg gagccccgcc cgccccttga tttttcgaaa
     1561 atacccatta tccttgcgga agaccacttg attaaaggag cggcgacatg attcgaccgg
     1621 tttggtggac ggcggccctg ttggcggtgg tgggggcgac ggcgtgcaaa aagaagacgg
     1681 aaatcaaaat cggcgccccc gttcccgtga gcatgttcaa agaagccccg gtcctgccgg
     1741 cccgggctgt tatcaacaat tcaccgacca cgccgccggc gcccccgccg gctcacatgg
     1801 aagaaccgcc cccgttgccg atgccggaca agccgcaaga gcgttcgggc gataaacttc
     1861 gtcgcgctcc gcccgacccg aactcctgat ccagaatccc ttcccgtcgc ccgtcgaggg
     1921 cgcggttgac aataacggaa tcgtgccata gactggggcg gtgcccaaac ccatgagaaa
     1981 aaccaggcgg aagcttccca tcggagtcac tctcacggaa ttgatgatcg ccgtggcggt
     2041 catttccgtc ggggctctgg gtttgttggg tgccttcggc ggcatcgccc ggtccgttca
     2101 atcgtcaaag gccaaaaccc tggccaccaa cctctgccag gaaaaaaccc agatcctgaa
     2161 gcagaaaaac tattaccggg ttcttgtcac gacggctccc gactacaaca cggatttccc
     2221 cagccccatc ccttacgatc ccagcacggg gtatttcccc cccgaaacca tcgtcgaggg
     2281 gggcatcacg tttacccgct tgacctttgt ggaggtcgcg caagaagtgg gcacggacat
     2341 cgtgacgctg gcccccacgt cgtccgatac cgggctcaag ttgatcaccg tgacggccct
     2401 gtggaccacc ggcaacgaac ccaaagaact ccacgtccgc acgctggtga cgaacccgga
     2461 cgccacggtc caaaacagcg ttttgaaagg ctttgtccaa cgcgcggggc cgatcgccat
     2521 tccaaacgcc gcggtgtcgg tctccgaaaa ttcagggtgg gtggacacca cggacgggac
     2581 ggggctctac acgatcaacc tgttcccggg gtcctacaac attctggtgg aagcccccgg
     2641 gtattttccc cagctcgtcg cggtcactgt tcccgccaac gcgacggtca accagaacat
     2701 cacgctggcg ccgatgacca ctcaaacggt gcaggggtac gcttggatcc gggaccacct
     2761 ggtgatttct caagtggtcg ccagttcgga atccgccgcg gctccggggt tttaccagga
     2821 atacgtggag gtttttaatc ccaccaccag caccgtgcaa atgaaggggc gtttcggcct
     2881 gaagtttcag cgccgcgtcg cccaggacgc tctgccgcgg gtcatcgaca tcgattacga
     2941 ctacaaaacg cccgtgtcga cgaacatcgc cccgggcggt tattacctgt tcgccaacac
     3001 ct
//
LOCUS       DAOQLS010000195         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_18545_length_2997_cov_4.864378, whole genome shotgun sequence.
ACCESSION   DAOQLS010000195 DAOQLS010000000
VERSION     DAOQLS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18545_length_2997_cov_4.864378"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PK362_04710"
     CDS             <1..744
                     /locus_tag="PK362_04710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC74170.1"
                     /translation="ILARYDDGYFHCGTPLAGGYEDYARDEPLIRRTFARRWTDFQAL
                     GAEPPVRRVLDVGCATGVFLDFMREKGWEAEGLEIAPGPRRAAENRGFRVHDRELTAA
                     GLEAGRFGWITAWDVIEHLPDPGAALRECHRLLAPGGLLTLSTPDASAPLARCLGRRW
                     LGYRCVGEHLYFFGRNTLAEWLRRTGFEVLAFSSVGKYLALDRLATRLAFYTRIFRPL
                     AGWKKRAGGFPVFYINSGDTMSVVARKVG"
     gene            complement(757..2076)
                     /locus_tag="PK362_04715"
     CDS             complement(757..2076)
                     /locus_tag="PK362_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNC74171.1"
                     /translation="MRRTAAGCLVLLASGAALRAATLESRLGTSFGITANAGTDGADP
                     VVTARSVASGDMALPLWSELHSAPMMAALVARANQDRALGLKVLFGFSPTSINGLRTE
                     IDLPPWVAAGCGVSTATVSFSNPCVKNYFIADATTVAAALQPDYFHVATEINTLILRQ
                     VAQPADLEYVHFGQLYQQVYSAIKAVRPTCQVFVSFQYELQLRFERDNPGVWNSLLDV
                     YRGPGVSKLDVVGYTSYPCQNGFSDSKFAQAWQIPGNYYAPAAAHLTGAERTFFSEVG
                     WPSRGSGTESSQRGFVERLPELMAPARPVFVVWTLLHDVPSAVFSGNVDLASVGVRAE
                     DGSPKPAWEVLTGAVAGDPFRNTRAYPSPFKPGASPWVLTGLPPNLPFKIYDLTGRAV
                     VSLTTNDFGGAQWDGRDGGGRLLESGLYFVLLRSDGRTKKIRLVLQR"
     gene            complement(2118..>2997)
                     /locus_tag="PK362_04720"
     CDS             complement(2118..>2997)
                     /locus_tag="PK362_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNC74172.1"
                     /translation="MTDAKVDLGKKLYFDKRLSADGTISCATCHNPKKGWTDQLPVSA
                     GIKGQKGGRSAPTVLNSAFMDLQFWDGRAPSLEEQAKGPIQNPIEMGFTHAAMVTTLT
                     GVAGYKPLFKAAFGTEEINVDRVAKAIAAFERTVLTGNAPYDRSQAGDDKALSPAALR
                     GLALFFGKANCSVCHSGFNFSNSDFHNLGVGMGRKDPDLGRFVETKVESHRGAFKTPT
                     LRNLRDTAPYMHDGSQKTLEEVVEFYNKGGEKNPWLDKEIRPLKLTAAEKKDLIEFLG
                     ALNGDKVDTPEPVLPQ"
BASE COUNT          524 a          942 c          993 g          538 t
ORIGIN      
        1 atcctggccc gttacgacga cggatatttc cactgcggga cgcccctggc ggggggctac
       61 gaagattacg cccgggacga acccctgatc cgccggacct tcgcccgccg gtggacggac
      121 tttcaagccc ttggggccga acccccggtg cgccgcgtgc tggacgtggg gtgcgccacg
      181 ggtgtgtttt tggatttcat gcgggaaaaa ggctgggagg cggaagggct ggagatcgcg
      241 ccgggacccc gccgcgcggc ggaaaaccgg gggtttcggg tgcacgatcg ggaattgacg
      301 gcggcgggac tggaggccgg tcggttcggc tggatcacgg cctgggacgt gatcgaacat
      361 ttgccggacc cgggagccgc cctgcgggaa tgccatcggc tgttggcgcc ggggggcctg
      421 ttgaccctgt cgacgccgga cgcctcggcc cccctggccc ggtgcctcgg gcgtcgctgg
      481 ctgggttacc gctgcgtggg agagcatctc tatttcttcg ggcggaacac cctggccgag
      541 tggctccgcc ggaccggatt cgaggtcctc gccttttcgt ccgtcggaaa atacctggcc
      601 ctggaccggc tggccacgcg cctcgctttt tatacccgga ttttccgtcc cctagcgggg
      661 tggaaaaagc gggccggggg cttccccgtt ttctacatca attccgggga cacgatgtcg
      721 gtggtggcgc ggaaagtcgg ctgaagtccc gccggatcag cgctgaagga ccaggcggat
      781 ctttttggtt ctgccgtcgg agcgaagcag gacgaaataa aggccgctct ccagaagccg
      841 tccgccgccg tcccgcccgt cccactgggc cccgccgaaa tcgttggtcg tcagggacac
      901 caccgcccgg ccggtcaaat cgtaaatttt aaaggggaga ttcgggggca gtcccgtcaa
      961 cacccagggc gaggccccgg gcttgaaagg gctgggatag gcccgggtgt tccggaaggg
     1021 gtcgccggcg accgcgcccg tcaacacctc ccaagccggt ttgggcgacc cgtcctccgc
     1081 ccgaaccccc acgctcgcca aatccacgtt ccctgaaaaa acggcgctcg ggacgtcgtg
     1141 caacaacgtc cacaccacaa acaccgggcg ggcgggggcc atcaactcgg gcaaccgctc
     1201 cacaaatccc cgctggctgg attcggtccc ggacccccgg ctgggccaac ccacctccga
     1261 gaaaaacgtc cgttccgcgc ccgtcaaatg ggccgccgcc ggggcgtaat aatttcccgg
     1321 gatctgccag gcctgggcga atttcgaatc gctgaacccg ttctggcagg ggtaggaggt
     1381 gtagccgacc acgtccaatt tggagacgcc ggggccccga tagacgtcca ggagagagtt
     1441 ccaaactccc gggttgtccc gttcgaaccg aagttgcaac tcgtactgga aggaaacgaa
     1501 aacctggcag gtcggtcgca ccgccttgat ggccgagtag acctgctgat aaagctggcc
     1561 gaaatggacg tattccaggt ccgccggctg ggccacctgg cggagaatca gggtgttgat
     1621 ttccgtggcc acgtggaaat agtccggctg aagggcggcg gccacggtcg tggcgtccgc
     1681 gatgaagtaa ttcttgacgc aggggttgga aaaggaaacg gtggcggtgg aaaccccgca
     1741 cccggcggcg acccaggggg gcaggtcgat ctcggtgcga aggccgttga tgctggtggg
     1801 gctgaacccg aacaggactt tgagccccag ggcccggtct tgattggccc gggccacaag
     1861 ggcggccatc atcggcgcgg agtgaagttc gctccagagc gggagggcca tgtcccccga
     1921 ggccacggac cgcgcggtca ccaccgggtc cgcgccgtcg gtcccggcgt tggccgtgat
     1981 gccgaaggac gtccccaaac gggactccaa ggtcgccgcc cgaagggccg cgcccgacgc
     2041 caggagaacc agacaccccg ccgctgttcg tctcacggcc cccattaaac caaatggccc
     2101 cggggcggga cagcgggtta ttgagggaga accggttccg gcgtgtcgac tttgtcgccg
     2161 ttcagggcgc cgaggaattc gatcaaatcc tttttctccg ccgcggtgag cttcaacgga
     2221 cgtatttcct tgtccaacca gggatttttc tcgccgccct tgttgtaaaa ctccacgacc
     2281 tcctccaggg ttttctggga cccatcgtgc atgtagggcg ccgtgtcccg gaggttccgc
     2341 agggtcgggg ttttaaaggc cccgcgatgg gactcgacct tggtttccac aaaacggccc
     2401 aggtcggggt ctttccgccc catgccgaca cccagattgt ggaagtccga gtttgaaaaa
     2461 ttgaagcccg aatggcaaac gctgcaattg gctttcccaa aaaacagggc caatccccgc
     2521 agagccgcgg ggctcagggc cttgtcgtcg ccggcctggc ttcggtcgta gggggcattg
     2581 ccggtcaaca ccgtccgctc aaaagccgcg atggccttgg ccacccggtc cacgttgatt
     2641 tcctcggtgc cgaaggccgc cttgaacaac ggcttgtagc ccgcgacgcc ggtgagcgtc
     2701 gtgaccatgg ccgcgtgggt gaaacccatt tcgatgggat tttgaatggg tcccttggcc
     2761 tgctcctcca gggaaggcgc ccggccgtcc cagaactgga gatccatgaa ggcgctgttc
     2821 aacacggtgg gggcactgcg cccgcccttt tggcctttga tgccggcgga cacgggcagc
     2881 tggtcggtcc agcccttttt cggattgtgg caggtggcgc aactgatcgt tccatcggcg
     2941 gaaaggcgct tgtcgaaata aagttttttg cccaggtcga ccttggcgtc ggtcatc
//
LOCUS       DAOQLS010000196         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_18621_length_2991_cov_4.049728, whole genome shotgun sequence.
ACCESSION   DAOQLS010000196 DAOQLS010000000
VERSION     DAOQLS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18621_length_2991_cov_4.049728"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK362_04725"
     CDS             <1..655
                     /locus_tag="PK362_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HNC74173.1"
                     /translation="LKSLGVERILGIGAVGSLKEELAPRHFVVPDQVFDRTKFRRTSF
                     FGDGVVGHVSVDQPFCAGLSQTLFEAARDIGVTAHRGGTYVCIEGPSFSTKAESQVNR
                     SHGFSVVGMTLVPEYKLAREAEICYATVSLVTDYDVWKEGEEVSVEKVIATLHANVDN
                     VKKLVRRVLPRLGADRTCACASALRHAVFTNPAAMKPATRKNLDLLIGKYLPAKSKG"
     gene            660..1481
                     /locus_tag="PK362_04730"
     CDS             660..1481
                     /locus_tag="PK362_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74174.1"
                     /translation="MLDDFRQLLPIFSNVVMPLLAGLIFFAMAKYVHHIAPLRTLITG
                     ELTYRGAYWGFLFLGVYLASRPIQILFPHPWPLIVNNIREFLMIAVFGPAVFLAMASL
                     VFGTENIPRRLIIAVLTLGVVLAIVFVVTNSFVIGGSEPIFKVGAWTAHDGLWFKNPD
                     LNRRAYMRILFAVRIIDPVCLVFLAGSLVLWHAKNYPVEKRVLYDNMPTKLYLLGAAT
                     YAFSLTMLLVGLIFLMAHIPNQWWIYYLGALAAGFLETLSLALPMRKHVQVSEHQ"
     gene            1478..2026
                     /locus_tag="PK362_04735"
     CDS             1478..2026
                     /locus_tag="PK362_04735"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC74175.1"
                     /translation="MTRFVEKPLRRRRVHKGRAVDFWVDTVRLSSGGTATREYLGHPG
                     AVAVVAVSKPGPDPEILLVRQYRYPVKETTLELPAGKIDPGEKPAVCVNRELEEETGV
                     RAGRVKKILAYWPTPAFANEVIHLYVARDLKPGRFSPDEDEIIEPIRLKLSEVLRRIR
                     AGKIKDSKTVIGLLAYDRWRDR"
     gene            2030..>2991
                     /locus_tag="PK362_04740"
     CDS             2030..>2991
                     /locus_tag="PK362_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNC74176.1"
                     /translation="MRRAVLLVLLILAARARGAVRINEIAFDQSAGPDWVELFNPDTS
                     AVLLDGWVLSDMDAASGNEIHLVFPTPLPSGAFLIVFVDAAGPSDFDFSDGAGAVYSG
                     TATTVNLAATEDELALFNGPGLASAALVDFVAWVTDGDYNGVADQTQALTAGIWTASA
                     AVTLSDLGNDYSIGRRRDGADADRPEDFEIFQRPTPGQSNVPPPPPDSHPLQIDPATR
                     SFSPFDPEVGHRAVRLFYNAGSPAVVKTIRIFDSRGRAVRDLLVEDVGPAGRNLAGSA
                     AGILEWDGKDNAGEILPVGIYVVFYEGADPSAGGLQRGRGVVALG"
BASE COUNT          497 a          995 c          894 g          605 t
ORIGIN      
        1 cctcaagtcg ctgggcgtgg agcggatttt gggcatcggc gccgtcggca gtctgaaaga
       61 agaattggcc ccccgccatt tcgtggtccc ggaccaggtc ttcgaccgca ccaaattccg
      121 acgaacgagt ttcttcggcg acggcgtcgt gggtcacgtt tccgtggacc agcccttttg
      181 cgcgggcctt tcccaaaccc ttttcgaagc cgcccgggac atcggcgtca cggcccaccg
      241 gggcggcacc tacgtctgca tcgagggccc cagcttttcc accaaagccg aatcccaggt
      301 caaccgctcc cacggatttt cggtggtggg catgacgctg gtgccggaat acaagctcgc
      361 ccgggaggcg gagatctgct acgccacggt gtccctggtg acggactacg acgtgtggaa
      421 ggagggcgag gaggtctccg tggagaaagt catcgccacc ctccacgcca acgtcgacaa
      481 cgttaagaag ctcgtccgcc gggtccttcc ccgcctgggg gccgaccgga cctgcgcctg
      541 cgcgtcggcc cttcggcacg cggtgttcac gaaccccgcc gccatgaagc cggccacccg
      601 aaagaacctg gaccttttga tcgggaaata ccttcccgcg aagtcgaaag gctgatccct
      661 tgctcgacga cttccgccag cttctgccca ttttttccaa cgtcgtcatg ccgctcctgg
      721 cggggctcat tttcttcgcc atggccaaat acgtgcacca catcgccccc cttcgcaccc
      781 tcatcaccgg ggaactgacc taccggggcg cctactgggg ttttctcttc ctgggcgttt
      841 atctggcttc ccggcccatc cagatcctct ttccccatcc ctggcccctg atcgtcaaca
      901 acattcggga attcctcatg atcgcggttt tcgggcccgc ggttttcctg gccatggcga
      961 gtctggtttt tggcacggaa aacattcccc ggcggctgat catcgccgtg ctgaccctgg
     1021 gggtcgttct ggccatcgtg ttcgtcgtca ccaattcctt cgtcatcggg ggttccgaac
     1081 ccatttttaa agtgggggcg tggacggccc acgacggcct gtggttcaag aaccccgatt
     1141 taaaccgccg ggcctacatg cggattcttt tcgcggtccg catcatcgac cccgtgtgcc
     1201 tggttttcct ggcggggtcc ctggtcctct ggcacgccaa aaactacccc gtggaaaagc
     1261 gggtcctcta cgacaacatg cccacgaagc tttacctcct gggagcggcc acctacgcct
     1321 tttccctgac catgctgttg gtcggcctga tttttctcat ggcgcacatc ccgaaccagt
     1381 ggtggatcta ctacctgggc gccctggccg ccggcttttt ggagacgctg tccctggccc
     1441 ttcccatgcg caagcacgtc caggtgagcg aacaccagtg acccgcttcg tcgaaaaacc
     1501 cctgcgccgc cggcgggtgc acaagggccg cgcggtggat ttttgggtgg acacggtgcg
     1561 tttgtcctcc ggcggcaccg ccacccggga atacctgggc caccccggcg ccgtggccgt
     1621 ggtcgcggtg tccaaaccgg gccccgaccc cgaaatcctt ctggtccggc aataccgcta
     1681 ccccgtcaaa gaaaccacct tggaactgcc cgccggaaag atcgatccgg gggaaaaacc
     1741 cgcggtctgc gtgaaccggg agttggagga ggaaaccggt gtccgggccg ggcgcgtcaa
     1801 gaaaatcctc gcctattggc ccacgccggc cttcgccaac gaagtgatcc atttgtacgt
     1861 cgcccgggac ctgaaacccg ggcggttttc tccggacgaa gacgaaatca tcgagcccat
     1921 ccgcctaaag ttgtccgagg tgttgcggcg gattcgcgcg ggaaagatca aggactccaa
     1981 aacggtcatc ggccttctgg cctacgaccg ttggcgcgac cgatagaacg tgcgccgggc
     2041 ggtcctcctc gttctgctga tcctggcggc gcgggcccgg ggcgcggtgc gaatcaacga
     2101 aatcgccttc gatcaatccg ccggtcccga ttgggtcgaa ctctttaacc ccgacacttc
     2161 cgccgtcctc ttggatggct gggttctgtc cgacatggac gccgcctccg gcaacgaaat
     2221 ccaccttgtt tttccaaccc cccttccttc cggcgccttt ctgatcgtct ttgtggacgc
     2281 cgccggcccg tcggatttcg atttttccga tggcgcgggc gcggtttaca gcgggacggc
     2341 gacgacggtc aatctggcgg ccaccgaaga cgaattggcc ctctttaacg gtccgggact
     2401 tgccagcgcc gccctggtgg attttgtggc ttgggtgacg gacggggact acaacggagt
     2461 cgcggatcaa acccaggccc tgaccgcggg aatctggacg gccagcgcgg ccgtgaccct
     2521 gtccgatttg ggaaacgact attcgatcgg tcgtcgtcgg gacggcgcgg acgccgaccg
     2581 gccggaggat ttcgaaatct ttcagcgccc gacgcccggc caaagcaacg tgcctccgcc
     2641 tcctcccgat tcccatcctc ttcaaatcga tcccgcgacg cgttcctttt cgccttttga
     2701 tccggaggtc ggccatcggg cggtgcgcct tttttacaac gccgggtcgc cggccgtcgt
     2761 gaaaaccatc cgaatctttg acagccgggg acgcgcggtt cgcgatctcc tggtggagga
     2821 cgtcgggccg gccgggagga acctggccgg ctcggcggcg ggaatccttg aatgggacgg
     2881 gaaagacaac gccggagaga tccttcccgt cgggatctac gtcgtttttt acgaaggcgc
     2941 cgacccctcg gcggggggcc tccaaagggg gcggggcgtc gttgctctgg g
//
LOCUS       DAOQLS010000197         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_18622_length_2991_cov_3.706063, whole genome shotgun sequence.
ACCESSION   DAOQLS010000197 DAOQLS010000000
VERSION     DAOQLS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18622_length_2991_cov_3.706063"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..334
                     /locus_tag="PK362_04745"
     CDS             137..334
                     /locus_tag="PK362_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1931 domain-containing protein"
                     /protein_id="HNC74177.1"
                     /translation="MAKKQIPDLVVKSKAKDLLAKAGCRSSSDVFDAVNGLIAWYLEQ
                     AAKRAKANGRQTVRGHDFMVE"
     gene            423..1205
                     /locus_tag="PK362_04750"
     CDS             423..1205
                     /locus_tag="PK362_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNC74178.1"
                     /translation="MKIPYFVVDAFTERPFGGNPAGVCPLEAWLPDSTLQSIAFENNL
                     SETAFFVADRDRFHLRWFTPTAEVDLCGHATLASAHVLFERLGHKKPSVQFETKSGAL
                     TVSRERDGYLGMDFPSLPPKPVEPPPGLRTALGTAPETVLAGMDYVAVYRDAQAVSRL
                     APDMDGLRGLDRRGVIVTAPGLEADYVLRFFAPKVGVPEDPATGSAQCLLAPYWAGRL
                     KKPALTVRQLSFRGGDMRCEVKGDRVLISGRSKVYSQGEICL"
     gene            1243..>2991
                     /locus_tag="PK362_04755"
     CDS             1243..>2991
                     /locus_tag="PK362_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNC74179.1"
                     /translation="MNGSTFQELIREKILILDGAMGTMLQKMNLTAADFGGPALEGCN
                     ENLILSKPSAVQAVHDGYLKAGADIIETNTFGSTPLVLAEYGLADQALEISRRGAQLA
                     KESAKKFSTPAKPRFVAGSMGPTTKTLYVTGGVTFDGLIETFEIQARGLIEGGSDLLL
                     VETAQDTLNIKAALIGIDRAFARTGQKIPVMVSGTIEPMGTTLAGQGVEALYYSLQQR
                     DLLSIGLNCATGPEFMTDHIRTLSDLSRFAVSCYPNAGLPDEEGRYNETPEMIAKKLE
                     RFVDSGWVNFLGGCCGTTFDHIRLIAQMAEGKKPRRAASATRFALSGLDPLVVTEEMR
                     PVLVGERTNVIGSRKFKNLIVEGKFEEASEIARHQVRAGAQIVDVCLANPDRDERADM
                     DKFLDFVTRKVKAPLMIDTTDAAVMELALKKCMGRSVVNSINLEDGEERFEKVVPLLK
                     TYGAAVVVGCIDEDKQQGMAVTRERKLAVAQRSYDLLTTKYGLEPEDIVFDPLVFPVA
                     TGDANYIGSARETIEGLRLIKRTFPRCKTILGVSNVSFGLPEAGREVLNAVYLYHCVQ
                     AGLDLAIVNSEKLARYP"
BASE COUNT          577 a          923 c          929 g          562 t
ORIGIN      
        1 gggaaggggt cggtttttcg aggatccatg ccggggtcag aataccgttt ttcctccggg
       61 ccaacaacgg ccgcctccct ttttgagcgc cgtcgtgata tcatgtacgc cgacattcca
      121 ttcgaaaggg gaactcatgg ccaaaaagca aattccggat cttgtggtga aatccaaagc
      181 gaaggacctt ctggcgaagg cgggttgccg ctcctccagc gacgtttttg acgcggtcaa
      241 tgggctcatc gcctggtatc tcgagcaagc ggcgaagcgg gccaaagcca acggtcgtca
      301 gaccgtgcgc ggccacgact tcatggtgga ataagtcccc cacccttcac gaactttcgc
      361 gcggggggcg ggtgccgaaa gcatccgccc cccgttcttt ttgaaattgg agcctccgaa
      421 aagtgaaaat cccctatttc gtggtcgacg ccttcaccga gcgccccttc ggcggcaacc
      481 ccgccggggt gtgccccttg gaggcctggc ttccggattc gacgcttcaa agcatcgcct
      541 ttgaaaacaa cctgtccgag acggcttttt tcgtggccga ccgggaccgg ttccatctgc
      601 gctggttcac gcccacggcg gaggtggatc tgtgcggcca cgcgaccctg gcctcggccc
      661 atgttctctt tgaacgtttg gggcataaaa agccctcggt ccaatttgaa acgaaaagcg
      721 gggcgttgac ggtgtcccgg gagcgggacg gttatttggg aatggatttt ccctccttgc
      781 cgccgaagcc cgtggagccc ccgcccggac tccggacggc cctgggcacc gcgcccgaga
      841 cggttttggc gggcatggat tacgtggccg tttatcggga cgcccaggcg gtttcccgtt
      901 tggcgcccga catggacggt ttacggggat tggaccgccg gggcgtcatc gtcaccgcgc
      961 cgggcctgga ggccgactac gtgcttcgat tcttcgcgcc caaggtgggg gtcccggaag
     1021 accccgccac gggctccgcc caatgcctcc tggcgcccta ttgggcgggc cggctcaaaa
     1081 aaccggccct gacggtgcgg cagctgtcgt tccggggcgg ggacatgcgg tgtgaagtca
     1141 aaggggatcg ggtgctgatt tcgggtcgat ccaaggttta ttcccaagga gagatatgtt
     1201 tgtgaatttt ttgttggggc gttttgaacg gggagcggcg cgatgaacgg ttcaacgttt
     1261 caagaactca ttcgcgagaa aatcctcatt ctcgacgggg ccatgggcac catgctccag
     1321 aagatgaatt tgacggcggc ggatttcggc ggccccgccc tggagggctg caacgagaac
     1381 ctgatcctgt ccaaaccgtc ggcggtacag gcggttcacg acggctattt gaaagccggc
     1441 gcggacatta tcgaaacgaa caccttcggc tcgacccccc tggtgttggc ggaatacggg
     1501 ctggcggacc aggccctgga aatcagccgt cggggcgccc aactggccaa ggagagcgcc
     1561 aaaaagtttt ccacccccgc caagccgcgg ttcgtggcgg ggtccatggg gcccaccacc
     1621 aagacccttt acgtcaccgg cggcgtgacc tttgacggtc tcatcgaaac ctttgaaatc
     1681 caagcccggg gtttgatcga gggcgggtcg gatctgttgc tcgtcgaaac cgcccaggac
     1741 accttgaaca tcaaggccgc cttgatcggc atcgaccggg ccttcgcccg gacgggacaa
     1801 aaaatccccg tcatggtgtc cggcaccatc gagcccatgg gcaccaccct ggcgggccag
     1861 ggcgtcgaag ccctctacta ttccctccag cagcgggacc tgttgagcat cgggttgaac
     1921 tgcgccacgg gtcccgaatt catgacggat cacattcgga ccctctccga cctttcccgg
     1981 ttcgcggtct cctgttaccc caacgccggt cttcccgatg aggaaggccg ctacaacgaa
     2041 acccctgaaa tgatcgccaa aaagctggag cggtttgtgg acagcggttg ggtgaatttc
     2101 ctcgggggct gttgcggcac gacctttgac cacatccggt tgatcgccca gatggccgaa
     2161 gggaaaaagc cccggcgggc cgcctccgcg acccgcttcg ccctttcggg cctggacccc
     2221 ctggtggtga cggaagaaat gcgtcccgtg ctggtggggg agcgcaccaa cgtcatcggc
     2281 agccgcaaat tcaagaacct gatcgtcgaa gggaaattcg aggaagcctc ggagatcgcc
     2341 cgccaccagg tccgcgcggg ggcgcagatc gtcgatgtct gcctggccaa cccggaccgg
     2401 gacgagcggg ccgacatgga caaatttttg gatttcgtga cgcgaaaagt caaagccccc
     2461 ctcatgatcg acaccaccga cgccgcggtc atggagctgg ccttgaagaa atgcatgggc
     2521 cgatcggtgg tcaactccat caacctggag gacggcgagg aacgcttcga aaaagtcgtg
     2581 cccctcctaa agacgtacgg cgcggccgtg gtcgtgggat gcatcgacga ggacaaacaa
     2641 cagggcatgg ccgtcacccg ggagcggaaa ctggccgtgg cccaacggtc ctacgacctg
     2701 ctcacgacaa agtacggcct ggaacccgaa gacatcgtct tcgatcccct ggtcttcccc
     2761 gtggccaccg gcgacgccaa ctacatcggc tcggcccggg aaaccatcga gggcctccgg
     2821 ttgatcaagc ggaccttccc ccgctgcaaa acgatcctgg gggtgagcaa cgtgtccttc
     2881 ggcctgccgg aagcgggccg ggaggtgttg aacgccgtct atctttacca ctgcgtgcag
     2941 gccgggctgg atttggccat cgtgaattcc gaaaaactgg cccgctaccc g
//
LOCUS       DAOQLS010000198         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_18746_length_2980_cov_4.500171, whole genome shotgun sequence.
ACCESSION   DAOQLS010000198 DAOQLS010000000
VERSION     DAOQLS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18746_length_2980_cov_4.500171"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..521
                     /locus_tag="PK362_04760"
     CDS             141..521
                     /locus_tag="PK362_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74180.1"
                     /translation="MRIHRRLGRRAALAGAMALAGLPLRALTLDTNGVIPRLFSPNGD
                     GINDVVYFSLSNPTLSDVTGRVLDVSGGEVSDLAPAGALGPTPESLMWDGRDRSGRVV
                     PSGPYVFEIQGDGQLISGVVVVVQ"
     gene            518..2119
                     /gene="traF"
                     /locus_tag="PK362_04765"
     CDS             518..2119
                     /gene="traF"
                     /locus_tag="PK362_04765"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNC74181.1"
                     /translation="MNSTFVSRATRALRLGLVLGLTASPLRAFDEFSVGARPAALSGA
                     FTAVADDVHSVYYNPAGLGLLPRPELTAYYARLFPNLSDQTRTALTFLGGASPLPFDG
                     RWGGVGLGYTEFRVDSLYKERQLALAYGRSFLADRLSLGLGLKSLQRVFGDTADTLNA
                     FSGNNPGNRTGAIDPVFQGGHGATAVGLDVGALYRLFPQWRLGLALANLNRPDLGLAN
                     DEKLPLITRMGVAFQPKFMTVSADVTRRTFLNSRPDNRLQLGVERGWLFRRYGTLSLR
                     GGAGFGGRDYRQVTLGAGYEVNGVVLDYVFTVPLGALDQTGNTHNIGLSYKFGRAPAE
                     DELLALIVEERDATARAEEALRLAEAESAFVKDERNRLLQEYTAEIDRLKKEAESKKP
                     APTPANRVLTPEERARLAREKALREYAAAFDAAYRGYQVHVERGATLTTRLEKLNALT
                     AAYRDKGIDLSRADKEMERVKTELAQVSTDYRLTLDFYRKNAAQGADAQEQISLLERM
                     IKKYTRAGIDLSEAQKELAKLKGGK"
     gene            2130..>2980
                     /locus_tag="PK362_04770"
     CDS             2130..>2980
                     /locus_tag="PK362_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNC74182.1"
                     /translation="MKIRTKITLSSLFFLAVSSGVGAWCYLNIQKEERAFDDSRRAAR
                     TRAAVADIDYFATRRVRALQNYVLLNDEAEKLQFQQAGTQVLQRLALWRKSAAHRETN
                     DADLPAVSAAFDALSGPAEKVETAMDKGRRTEAMSLVESEFVPASAGALKAIADIKNR
                     TEASALQSEQDMTAELRRNHWSLLAGMGLVVFFGLVFLVSFYRSVIQPIHLMKSWADR
                     VAQGERNVPWRFPGKNELTELATSLGEMAIQLTRPRAAPPAAVAAAASVAPAARVWPS
                     PTETAGKT"
BASE COUNT          537 a          948 c          962 g          533 t
ORIGIN      
        1 tccgatctcc tgggacggac gggataagac tggacatatc gttcccgcgg gggtatacat
       61 ttataagatc gaaggcgaag gacggacctt ctcgggcacc gttgtggtgg cgcaatgagc
      121 ccgcacccgg agagacaacg atgaggattc accgacggtt gggacgccgc gcggccttgg
      181 cgggggccat ggccctggcc gggttgccgc tgcgcgcgct caccttggac acgaacggcg
      241 tcattccccg acttttttcc ccgaacggcg acggcatcaa cgacgtggtc tatttttccc
      301 tcagcaaccc caccctctcc gacgtgaccg gccgggtgtt ggacgtgtcc gggggcgaag
      361 tgtcggacct ggcgcccgcc ggggccctcg gtcccacccc cgaaagtttg atgtgggacg
      421 gtcgggaccg gtccgggcgg gtggtcccct cgggtcccta cgtgtttgaa atccaaggag
      481 acggccaatt gatcagcggc gtcgtggtgg tggtccaatg aattcaacct tcgtttcgcg
      541 cgcgacccgg gcgctgcgcc tgggactggt cctcggattg acggcgtcgc ccctgcgggc
      601 tttcgacgag ttttccgtcg gcgcccgccc ggcggccctc tcgggagcct tcaccgccgt
      661 ggccgacgac gtgcacagcg tgtattacaa ccccgccggc ttgggcctcc ttccgcggcc
      721 ggaactgacg gcctattacg cccggctgtt ccccaatctc tcggaccaaa cccgaacggc
      781 gctcaccttt ctgggcggcg ccagtcccct tcccttcgac gggcggtggg gcggcgtcgg
      841 tctcggttac acggaattcc gcgtggactc cctgtacaag gagcgtcaat tggccctggc
      901 ctacggccgt tccttcctgg ccgaccgtct gtctctgggc ctcgggctca agtcgcttca
      961 gcgggtgttc ggcgacacgg ccgacacgct gaacgccttt tcgggcaaca acccgggcaa
     1021 ccggacgggg gccatcgatc cggtgtttca aggcggtcac ggggccaccg ccgtgggcct
     1081 ggacgtgggc gcgttgtacc ggctttttcc ccagtggcgg ctgggtctgg ccttggcgaa
     1141 tctaaaccgg cccgacctgg gcctcgccaa cgacgaaaaa ttgccgctca tcacccggat
     1201 gggcgtggcg ttccagccca aattcatgac ggtgagtgcg gacgtgaccc ggcggacctt
     1261 cttgaacagc cggccggaca atcgccttca attgggggtg gagcgcgggt ggctcttccg
     1321 ccgttacggc acgctgagtc ttcggggcgg ggccggtttc ggcgggcggg actaccggca
     1381 agtgaccttg ggcgccggtt acgaggtgaa cggcgtcgtg ttggattacg tcttcacggt
     1441 gcccttgggg gccttggatc agacgggcaa cacccacaac atcgggttgt cctacaaatt
     1501 cggccgggct ccggccgagg acgagttgtt ggcgcttatc gtcgaggaac gggacgccac
     1561 ggcccgggcg gaggaagccc tgcggttggc cgaggcggag tccgccttcg tcaaggacga
     1621 gcgcaaccgg ttgcttcagg aatacacggc ggaaatcgat cgactcaaaa aagaagccga
     1681 atcgaaaaaa cccgctccca cccccgccaa ccgggtgttg acgcccgagg aacgggcccg
     1741 gttggccagg gaaaaggccc tgcgggaata cgccgccgct ttcgacgccg cctaccgcgg
     1801 ctaccaggtt cacgtggaga ggggcgcgac gttgacgacc cgtctggaaa aattgaacgc
     1861 cttgacggcg gcctaccggg acaagggcat cgacctgtcc cgggcggaca aggaaatgga
     1921 gcgggtgaaa accgaattgg cccaggtgtc gacggattac cgcctcacgc tggatttcta
     1981 ccggaaaaac gccgcccagg gcgccgacgc ccaggagcaa atctccctgt tggagcggat
     2041 gattaaaaag tacacccgag ccggcatcga cctctcggaa gcccagaagg agctcgccaa
     2101 gctgaaaggc ggaaaatagt tttcgcccca tgaagatccg caccaaaatc accttgtcgt
     2161 ccctgttctt cctggccgtt tcctccggcg tgggggcctg gtgctacttg aacattcaaa
     2221 aagaggaaag ggcatttgac gacagccgtc gggccgcccg gacgcgggcc gcggtggccg
     2281 acatcgatta tttcgccacc cgccgggtgc gcgcccttca aaactacgtt ttgctgaacg
     2341 acgaagcgga aaaactccag ttccagcaag ccggaaccca ggtgcttcag cggctggcgc
     2401 tctggcgaaa atccgccgcc catcgggaaa ccaacgacgc cgatcttccg gccgtttccg
     2461 cggctttcga cgccctgtcc gggccggcgg aaaaagttga aacggccatg gacaaaggac
     2521 gacggacgga agccatgtcg ttggtggaat cggaattcgt ccccgccagc gccggcgccc
     2581 tcaaggccat cgccgatatt aaaaatcgaa ccgaggcctc cgccctccaa tccgagcagg
     2641 acatgaccgc cgagcttcga cggaatcact ggtccctctt ggccgggatg ggtttggtgg
     2701 tgttttttgg cctggtgttt ctggtgtctt tctaccggtc cgtgatccag cccatccatt
     2761 tgatgaaatc ctgggcggac cgggtcgccc agggggaacg aaacgtgccc tggcgtttcc
     2821 ccggaaaaaa cgaattgacg gaattggcga cgagcttggg cgaaatggcc attcaattga
     2881 cccggcccag agccgccccg ccggcggcgg tcgccgcggc cgcgtcggtc gctcccgccg
     2941 cccgggtctg gccctctccg acggaaacgg cgggaaaaac
//
LOCUS       DAOQLS010000199         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_18757_length_2979_cov_4.021888, whole genome shotgun sequence.
ACCESSION   DAOQLS010000199 DAOQLS010000000
VERSION     DAOQLS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18757_length_2979_cov_4.021888"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PK362_04775"
     CDS             complement(<1..717)
                     /locus_tag="PK362_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74183.1"
                     /translation="MMNVFFVLFLLFLPWLGCAQGKMPRLTEILSPAVETDGPTLKTS
                     QDAYFHDAFVEWTLSVPPGSTLFDKLSSSTAPWVTWVEKDGAPVTTIGMMREAPLVFS
                     TGTRSWSARWPVPWNAPDGTYAVRLDTAAWPAGEKTPAFPTFKIQSREFKPVPKGFGV
                     LTLEGHAPFHVFRAPDGQKRASAMAEWAEFMGADALVVQGAETSGFTVKLSTSFPWLT
                     RNEKGVIELGKACRERGLKLG"
     gene            complement(789..862)
                     /locus_tag="PK362_04780"
     tRNA            complement(789..862)
                     /locus_tag="PK362_04780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(826..828),aa:Arg,seq:ccg)
     gene            complement(889..>2979)
                     /gene="alaS"
                     /locus_tag="PK362_04785"
     CDS             complement(889..>2979)
                     /gene="alaS"
                     /locus_tag="PK362_04785"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNC74184.1"
                     /translation="GPSCRPENDCDRWMEVWNNVFTQFDRQPDGSLKPLPKKNIDTGM
                     GLERLTAVVQGVFTNFETDLFVPLIRQTEEILGKTSKGLTGAAIAPFRLIPDHARAST
                     FMIADGILPSNEGRGYVLRRLIRRAVRHGRLVGREKPFLFQLAQSVVDQMGGAYPDLT
                     AKRESIVSMIKQEEERFLETLESGTARLEELVSSAKSQGRKTLGGVEVFKLYDTYGFP
                     PDMTREILAERGLSFDEKELEAARREAQDTARGAWKGSGQEDTGLYAQLHRDLGDVLF
                     RGYDVSEVKSVPVTALLKEGKPVSTLSQGEEGEIALKETPFYPEGGGPVGDTGVLTGA
                     AGKAQVLDTQRPVDGFIVHRVKVLSGKISKGDAVAATVDGDRREAIRRHHTATHLLHA
                     ALRRVLGTHVTQAGSIVTPDKLRFDYTHTATPSLAELRRIEDDANRNVLRDIARPEKT
                     PEMSLAEAQKAGAMAFFGDKYGDKVRVVKFGEATTEVCGGIHVLRTGEIGLIKIVAES
                     SIGAGVRRMEAVAGIKALEYLQQMESTVAEAAEKLKASPSELVQRIDRSLQRQRQLER
                     EMDELRVKAAQGGGGAAAVEAREVGGARWLVQAAPGLDEKSLRSLSDRLKEKADGAGV
                     LVATGNDEKISFVVALPAALTQAGWHAGKIAQAVAAKIGGKGGGRPDFAQGGGKASLA
                     LDQLFGDLPELLRK"
BASE COUNT          515 a          934 c          965 g          565 t
ORIGIN      
        1 gcccagtttg agtccccgct cccggcaggc tttgccgagc tcgatgacgc ccttttcgtt
       61 gcgcgtcaac caaggaaaag acgtcgatag cttgacggtg aaaccgctgg tctccgcgcc
      121 ctgaaccacc agggcgtcgg cgcccatgaa ctcggcccac tcggccatgg cggaagcccg
      181 cttttgtccg tccggcgcac ggaagacgtg gaacggcgcg tggccctcca gggtgagcac
      241 gccgaagccc ttggggaccg gtttaaactc ccgggattga attttgaagg tgggaaaagc
      301 gggggttttt tcgcccgcgg gccaggcggc ggtgtccagg cggaccgcgt aggttccgtc
      361 gggcgcgttc caggggaccg gccatcgggc ggaccaggac cgggtccccg tgctgaaaac
      421 caggggggcc tcccgcatca tgccgatggt ggtgacgggc gcgccgtcct tttccaccca
      481 ggtgacccag ggggcggtgg aggacgacaa tttgtcaaaa agggtcgacc ccggggggac
      541 gctcagggtc cattccacaa aggcgtcgtg gaaataagcg tcctgggacg tttttagggt
      601 ggggccgtcg gtttcgacgg cgggggacaa tatttcggtg agacggggca ttttgccctg
      661 ggcgcaaccg agccagggca ggaagaggag aaagagaacg aaaaagacgt tcatcaccca
      721 aagacctcaa aacaccgatt gtttttcatc catacgccgt ccgcgtccgg ttggcggatc
      781 aaaaaaagcg tgcccggggg gagttgaacc ctcagccttt ggctccggag gccaacgctc
      841 tatccaattg agctacgggc acaaaaatgt ttcaggaagc ggattttact acttgcgaag
      901 caattcgggg aggtcgccga aaagctggtc caaagccagg gacgctttcc cgccgccctg
      961 ggcgaaatcg ggccgaccgc cgcccttgcc gccgatcttg gcggccacgg cctgggcgat
     1021 tttccccgcg tgccaaccgg cctgggtcaa tgccgcgggc agagccacca caaaagaaat
     1081 tttttcgtca ttgcccgtgg ccacgagcac cccggccccg tcggcctttt ccttcaggcg
     1141 atcggagagg gaccgcaggg atttttcgtc caagcccggt gcggcctgca cgagccaccg
     1201 ggccccgccg acttcccggg cttcgacggc ggccgcgccc ccgccgccct gggcggcctt
     1261 gactcgaagt tcgtccatct cccgctccaa ctggcgctgg cgttggagcg accggtcgat
     1321 gcgttggacc agttccgacg gggaggcctt gagcttctcg gcggcttccg ccacggtgct
     1381 ttccatctgt tgcaaatatt ccagggcttt gatgccggcc acggcctcca tgcgccggac
     1441 gcccgccccg atggaggatt cggccacgat tttgatcagg ccgatttccc cggtgcgaag
     1501 aacgtggatg ccgccgcaca cttccgttgt ggcctcgccg aacttcacaa cccggacctt
     1561 gtctccgtat ttgtccccga aaaaagccat ggcgccggct ttctgggctt cggccaggga
     1621 catttccggc gtcttttccg gccgggcgat gtcccgaagg acgttgcggt tggcgtcgtc
     1681 ttcaatgcgg cgaagttccg ccaaactcgg ggtggcggtg tgggtgtagt cgaaacggag
     1741 cttgtccggc gtgacaatgg acccggcctg ggtcacgtgg gtgcccaaaa cccggcgcag
     1801 ggccgcgtgg agcaaatggg tggccgtgtg gtgccggcga atggcttccc ggcgatcgcc
     1861 gtccacggtg gccgcgaccg cgtcgccctt cgaaattttt cccgaaagga ccttcacccg
     1921 gtgaacgatg aacccgtcca cggggcgctg ggtgtccagc acctgggctt tgcccgcggc
     1981 ccccgttaga acgccggtgt cgcccacggg accgccgcct tcgggataaa agggcgtttc
     2041 cttcaaagcg atttccccct cctccccctg ggaaagggtc gatacgggtt tgccctcttt
     2101 caggagagcc gtgactggaa cgcttttcac ttccgacacg tcgtagcccc ggaaaagaac
     2161 gtcgcccaaa tcccggtgca gttgggcgta cagtccggtg tcttcctggc ccgaaccctt
     2221 ccaagcgccc cgggcggtgt cctgggcttc ccggcgggcg gcttccaatt ccttttcgtc
     2281 gaaggacagg ccccgctcgg ccagaatttc ccgcgtcatg tccgggggaa acccgtaggt
     2341 gtcgtagagt ttgaacacct cgacgccccc gagggttttt cgaccctggg acttggccga
     2401 ggaaaccaat tcttccagcc gggccgtgcc gctctccagg gtttccaaaa agcgctcttc
     2461 ctcctgcttg atcatggaga cgatgctttc ccgtttcgcc gtcaaatcgg ggtaggcgcc
     2521 gcccatttgg tccacgacgg actgggccaa ctggaacaaa aagggctttt cccgcccgac
     2581 caaacggccg tggcgcacgg cccggcggat gagccgcctt aaaacgtagc cccgcccctc
     2641 gttggaggga aggatgccgt cggcgatcat gaaggtggag gcccgggcgt ggtccgggat
     2701 gagacggaag ggggcgatgg ccgcccccgt gaggcctttg gaggttttgc ccaggatctc
     2761 ctcggtctgg cggatcaggg ggacaaagag gtccgtttca aaattggtga agacgccctg
     2821 gaccacggcg gtcaggcgct ccagccccat gcccgtgtcg atgttctttt tcggaagggg
     2881 cttgagggag ccgtcgggct ggcggtcgaa ctgggtgaag acgttgttcc acacttccat
     2941 ccagcggtcg caatcgttct cgggccggca ggaaggacc
//
LOCUS       DAOQLS010000200         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19087_length_2950_cov_3.906736, whole genome shotgun sequence.
ACCESSION   DAOQLS010000200 DAOQLS010000000
VERSION     DAOQLS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19087_length_2950_cov_3.906736"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PK362_04790"
     CDS             <1..555
                     /locus_tag="PK362_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNC74185.1"
                     /translation="MELATLATSFYALRKIRFSFLFLPAAIALWFFSMDVAEFFMRSR
                     LGGGDRAWVSVGVGLLILILSRSVEKRVTGMDSFWLYLAGLLAFWGGLTSLPSHGEFG
                     RVVYAGINLGLIGVGIYLNRKTFAVFGAMGVYGYVGHLAWSVFKNSPAFPLVLAFVGL
                     MMILTTVLFQKNQGRLRRWMGLPS"
     gene            576..1298
                     /locus_tag="PK362_04795"
     CDS             576..1298
                     /locus_tag="PK362_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74186.1"
                     /translation="MTILLFLGSVVRAQDTVVLVHGILNKPFVMKAIEHRLKKEGFEV
                     INWGYASTEKTIEEYAADLKADLDVRRTTGTLHFVGFSLGSIITRYYLAHTPPAGVGR
                     FVQIAPPNHGSKWIDRLYTFKAFRWVYGTTAIEQLKDGSGFFKTCGVPACPLGIIVGG
                     NGTSAGYNPVLEGDDDGSVALASAVLPEAKDVAQVFAEHTLLLASPAVGRYAAAFLKT
                     GKFPADARRPRPIPSIVTGGSK"
     gene            1295..>2950
                     /locus_tag="PK362_04800"
     CDS             1295..>2950
                     /locus_tag="PK362_04800"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNC74187.1"
                     /translation="MIGEFLLLAALGILVLPVIALVFSVKALRAARRVEDRLDGLRGA
                     LRAGASTLRESAPPPVRETPGPSVDPRAVQRAVDLSPSPAVDPYGPPIAGSPAPGLPV
                     GGPPEAKPPTPSPRPPLATPPPIYVGDVEPEAPTAPPVLERPSIDWEKFTGVKLFSWI
                     GGFALFLGAAFFAKYSMDHGWITPAMRVAFGFAAGTASLLVGLKLKDRFAVTAQTLVA
                     AGAGILYTDAFAAHAFYGFIGPVETFVFLALVTVLTFFLSVRLDSRYIALLALVGGFL
                     TPPILSTGVDRALGLFAYVTLLDLGLLAVVLSRGWGFLWGLAAFGTAVMQWGWADHFL
                     VVEKLPVAAAVFLWFPILFVAARFVAERRGMDLAPLRHAAGLLALLSVLFGGNTLDRF
                     GAQPALPFLIILVADALTATLAVRGGSGSRYHLAAGILAYLTLFGWTSSRLTAETFPM
                     ILGVVLIFGALHGALAVVLNRRGAPKDVVILGNLFPVALLLPLAMSLMKEFGSPALLW
                     PVLFGLNAVAILVALATGFLWGVAAALGFTFLTVLVWIDRLTVD"
BASE COUNT          392 a          958 c          919 g          681 t
ORIGIN      
        1 atggaattgg caaccctggc cacctccttt tacgctcttc gaaagatccg cttctccttc
       61 ctcttcttgc ccgccgccat cgccctctgg tttttctcca tggacgtcgc cgagtttttc
      121 atgcggtcgc ggctcggagg aggcgatcgg gcttgggtca gtgtcggggt gggcctcttg
      181 atcttgatcc tgagccgttc cgtggaaaaa cgggtcacgg gcatggattc cttttggctt
      241 tatttggcgg ggctcctggc cttctggggc gggctcacct ccctgcccag tcacggggaa
      301 ttcggacggg tcgtctacgc ggggatcaac ctggggttga tcggggtggg gatctattta
      361 aaccgaaaaa ccttcgcggt cttcggcgcc atgggcgtgt acggctacgt gggtcatctg
      421 gcctggtcgg tgtttaaaaa cagtccggcc tttcccctgg ttctggcttt cgtcggcctg
      481 atgatgatct tgaccacggt gctctttcaa aaaaaccagg ggcggttgcg ccggtggatg
      541 gggttgccgt cgtgagtcgt tcagcgcctt ggacgatgac gattcttttg tttctgggtt
      601 ccgtggtccg ggcccaggac acggtggtgc tcgtccacgg gattctgaac aagcccttcg
      661 tgatgaaggc catcgaacac cgcttgaaaa aagagggttt tgaggttatt aactggggat
      721 acgccagcac cgagaaaacc atcgaagaat acgccgcgga cttgaaggcc gatctcgatg
      781 tccgtcgcac gaccgggacc ctccattttg tcgggttcag cctggggtcc atcatcaccc
      841 ggtattacct ggcccacacg cctcccgccg gggtcggccg gtttgtacaa atcgcgccgc
      901 cgaatcacgg ctccaaatgg atcgatcggt tgtacacctt taaagccttt cgctgggtgt
      961 atgggacgac ggccattgag caattgaaag acggcagtgg tttttttaaa acctgcggcg
     1021 ttcccgcttg tccgctgggc atcattgtcg gcggaaacgg cacctccgcg ggctacaacc
     1081 ccgtgttgga gggggacgac gacggttcgg tggccctggc cagcgccgtt ctgcccgaag
     1141 cgaaagacgt ggcccaggtc ttcgccgagc acaccttgtt gttggcctcc cccgccgtgg
     1201 gtcgctacgc cgcggcgttt ctcaaaacgg gcaaattccc cgcggacgcc cggcgtcccc
     1261 ggccaatccc ttcaatcgtt accggaggtt ccaaatgatc ggtgaattcc ttttgctggc
     1321 cgccctgggc attctggttc tgcccgtgat cgccctcgtt ttttccgtca aggcccttcg
     1381 agcggcccgt cgggtggagg accggctgga cggccttcgg ggcgccctgc gcgccggcgc
     1441 ctccactctc cgggagtccg ctccgccccc ggtccgggaa acccccgggc cttcggtcga
     1501 cccccgggcg gttcaacggg cggtggatct ttccccgtcg cccgcggtcg atccctacgg
     1561 gccgccgatc gccggttccc cggcccccgg attgccggtg ggcggtcccc cggaagcgaa
     1621 acccccgacg cccagccccc gcccgcctct cgcaacgccg ccgcccattt acgtgggcga
     1681 cgtcgaaccc gaggcgccca cggccccgcc ggttctggaa cggccgtcca tcgactggga
     1741 aaaatttacc ggcgtcaaac tcttcagctg gatcggcggc ttcgccctct ttttgggcgc
     1801 ggcctttttc gccaaatatt ccatggacca cggctggatc accccggcca tgcgggtggc
     1861 tttcggattc gccgccggga ccgcttcgct cctcgtcggc ctcaagctca aagaccgctt
     1921 cgccgtcacg gcccaaaccc tcgtggccgc gggggcggga atcctgtaca cggacgcctt
     1981 tgccgcccac gctttttacg gattcatcgg gcccgtggaa accttcgtgt ttttggccct
     2041 cgtgaccgtc ctcacgttct tcctgtcggt gcgtttggat tcgcggtaca tcgccctgct
     2101 ggccctggtg ggcggctttt tgaccccgcc catcttgtcc acgggcgtgg accgggccct
     2161 gggccttttc gcctacgtga cgctgctgga cctgggcctt ctggccgtgg tcttgagccg
     2221 ggggtgggga ttcctctggg gtctggccgc tttcgggacc gccgtcatgc aatgggggtg
     2281 ggcggaccat tttctggtcg ttgaaaaatt gcccgtggcc gccgcggtct ttctctggtt
     2341 cccgatcctc ttcgtggccg cgcgcttcgt ggccgaacgt cgggggatgg atttggctcc
     2401 cctgcggcac gcggcgggcc tgctggccct cctgtcggtt ctcttcggcg ggaacacgct
     2461 cgatcgtttc ggggcccagc cggccctgcc gtttctcatc attctcgtcg ccgacgcctt
     2521 gacggcgacc ctggcggtgc ggggaggctc gggatcgcgc tatcacctgg ccgccgggat
     2581 tctggcctac ctgaccctct tcggctggac ctcgagccgt ctgacggcgg aaacctttcc
     2641 gatgattctc ggcgtcgtcc tgattttcgg cgcccttcac ggcgcgttgg ccgtggtgct
     2701 caaccgtcgg ggcgccccca aggacgtggt gattttggga aaccttttcc ccgtggccct
     2761 tctcctgccg ctcgccatga gtttgatgaa ggagtttgga tccccggcgc tcctttggcc
     2821 ggtgctcttc ggcttgaacg ccgtcgccat cctggtcgcc ctggccacgg gatttttatg
     2881 gggcgtggcg gcggccctgg gtttcacctt cctcacggtt ttggtctgga tcgaccggtt
     2941 gacggtggac
//
LOCUS       DAOQLS010000201         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19126_length_2947_cov_3.309474, whole genome shotgun sequence.
ACCESSION   DAOQLS010000201 DAOQLS010000000
VERSION     DAOQLS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19126_length_2947_cov_3.309474"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2947)
                     /locus_tag="PK362_04805"
     CDS             complement(<1..>2947)
                     /locus_tag="PK362_04805"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNC74188.1"
                     /translation="LVLRAHSALGAGAKETLKDIAGLTERHVRLALVLALADVADGYR
                     DMHRPYIQALVRQSGFPTIGQLEREFLKYTGRLEGSPFAGALPDPAAVFARASAEPWF
                     AVATERTLIEHVAGLVQTGAAGLSGADKVRLLAREIGSHRLTELDTPADQWWPALVDA
                     LFLRGAAGDGRGAVLKSLLADLRTETAAGRLDARWRLAELTAALLSPAKPGPAAPREI
                     LLAREWTSEKVLAKDIDATARAVRELAAQFPSVAPAALSSLTSYALGETEGHGAWDGS
                     PARLELSRRGDTLRLRIENDGGYPLPNQPPKNIRNRSFTPATAEPLIYSSRGVESGSG
                     AGTAYIAVYFNQIYQREVPKELTSRARLAWTQRTLGPNRYRIALTVNIPLHSAVAARG
                     GAIPAVTAYWEPKFVSLLGERWGRYLYRAVAGPTLEDLFGGLGNSESINSALLLPMVV
                     GAVMNLSGDPVLGAFVPAFILLFNRLLFVVRHPEGQRTEAGFIAVLGGAGAFAVAWMG
                     AFSPVAAALGFLAGWSFHVATNAVSNLSVLFQGKPREPSAPSTPARTKIGLVLLMMGT
                     LFLSPMYRGGGSALIGAAADRWAGWTIAAAGLLVLVTSGNPLMNAKRGLDRLLTSAFA
                     RGISNRWTSLVRWFGRNKTPLLAAGAAVALGSLFGPSNPELMFAAAATTGIPLIRRSE
                     RRDLNIASVSVELAPPHGEVNQQIRGLMAFGHAILRRDAAGTWTVDNQRGALTLSDRD
                     AASLSKDPVLNDFEIHFVGDGAFRYLHVWPWGAQEGHGGGLSTVGTKNPVKRVFIARG
                     LWESNRERALTVLRHEATEVNLWWEQATKLEDEKQIVNTDRKAEALAAVAALRGRPRK
                     KSDVMVAADAKLPTIREWIRENALGAVVALTSRFHNMANRRYRTEHPLDFIPLPNTLN
                     GAVAMVSWEHRDFFKRGGLGDAISEASYNLAKLGQDVDLYMPMHKEMEGKAAWEDTGF
                     TVPVVSAGDR"
BASE COUNT          537 a          965 c          965 g          480 t
ORIGIN      
        1 ccggtccccg gcggagacga cgggaacggt gaacccggtg tcctcccagg cggctttgcc
       61 ttccatctcc ttgtgcatgg gcatgtacaa gtccacgtcc tggcccaact tggccaggtt
      121 gtaggacgct tcgctgatgg cgtcgccgag gcccccgcgt ttaaagaagt cccggtgctc
      181 ccaactgacc atggcgaccg cgccgttcaa cgtgttgggc aacggaataa agtccaaggg
      241 gtgttccgtc cggtagcggc ggttcgccat gttgtggaaa cggctggtca gcgccaccac
      301 ggcgcccagg gcgttttccc ggatccattc ccgaatggtg gggagtttgg cgtccgccgc
      361 caccatcacg tccgattttt tccggggacg cccgcggagg gccgccacgg cggccagggc
      421 ttcggccttc cggtcggtgt tgacgatctg tttttcatcc tccaacttgg tggcctgttc
      481 ccaccacaaa ttgacttcgg tggcttcgtg ccgcaacacc gtaagggccc gctcccggtt
      541 ggattcccag aggccccggg cgatgaagac gcgcttcacg gggttcttgg ttcccaccgt
      601 gctgagcccg ccgccgtggc cttcttgggc cccccagggc cacacgtgca gatagcggaa
      661 ggccccgtcg cccacgaagt ggatttcaaa atcgttcaac accgggtcct tggacaagga
      721 agccgcgtcc cggtcggaca acgtcaacgc gccccgttgg ttgtccacgg tccaggtccc
      781 cgccgcgtct cggcggagga tcgcgtggcc aaaagccatg agcccgcgga tttgctgatt
      841 gacttccccg tggggcggcg ccaattcgac gctgaccgag gcgatgttga ggtcccgccg
      901 ttcggaccgg cggatcaggg ggatgcccgt ggtggcggcc gcggcgaaca tcaattcggg
      961 gttggagggc ccgaaaaggg atcccagggc caccgccgcc cccgccgcca gcaagggcgt
     1021 tttgttccgg ccgaaccagc ggaccaggct cgtccagcgg ttggaaattc cccgcgcgaa
     1081 ggcggaagtc aaaagacggt cgaggccgcg cttggcgttc atcaacggat tgcccgaggt
     1141 gacgaggacc aggagtcccg ccgccgcgat cgtccaaccg gcccaacgat cggccgccgc
     1201 cccgatcaaa gcgcttccgc cgccccggta catgggcgaa aggaacaggg tccccatcat
     1261 caaaagcacg aggccgattt tggtccgggc cggggtcgac ggcgccgacg gttcccgggg
     1321 tttcccttga aaaaggaccg acaaattgga cacggcgttg gtcgcgacgt ggaaggacca
     1381 ccccgccagg aacccgaggg ccgccgccac gggagagaaa gcgcccatcc aggcgacggc
     1441 gaaggccccg gcgccgccca ggacggcgat gaaacccgct tccgtgcgct ggccttcggg
     1501 atgacggacc acgaacaaca atcggttgaa aaggaggata aaggccggaa cgaaggcccc
     1561 caggaccgga tcgcccgaaa ggttcatcac ggcgccgacc accatcggca aaagaagggc
     1621 gctgttgatg gattcggaat tgcccagccc gccaaacagg tcttccaggg tgggcccggc
     1681 caccgcgcga taaaggtacc ggccccaacg ttcgcccagc agggaaacga acttgggttc
     1741 ccaatacgcc gtgaccgcgg gaatcgcccc gccgcgggcg gcgacggcgg aatgaagcgg
     1801 aatgttcacc gtcagggcga ttcgatagcg gttggggccg agggttcgtt gggtccaggc
     1861 caagcgggcc cgggaggtca actccttcgg cacctcgcgt tgataaattt gattgaaata
     1921 aacggcgatg taggccgtgc cggcgcccga tccggactcc accccccggg aactgtaaat
     1981 caacggctcg gcggtcgcgg gagtaaagga tcgatttcga atatttttcg gcggttggtt
     2041 gggaagcggg taacccccgt cgttttcaat tcgcagacgc agggtgtccc cacgacggga
     2101 tagctccaac cgggccgggg aaccgtccca ggcgccgtgt ccttcggttt cccccagggc
     2161 gtagctggtc aacgacgaca aggccgccgg ggccaccgaa gggaattgcg cggcaagttc
     2221 tcgaaccgcc cgggccgtcg cgtcgatgtc cttcgccaaa accttttcgg acgtccattc
     2281 ccgggccaat aaaatttccc ggggcgccgc cggcccgggt tttgcggggg aaagaagggc
     2341 ggccgtcaat tcggccagcc gccaccgcgc gtccaatcgt cccgcggccg tttcagtgcg
     2401 gagatcggcc aacagggact ttaacaccgc cccccgcccg tcgccggccg ccccgcgaag
     2461 gaaaagggca tcgaccaggg cgggccacca ttgatcggcg ggggtatcca attccgtcaa
     2521 acggtggctt ccaatttccc gcgccagaag ccggactttg tccgccccgg aaagacccgc
     2581 cgcgccggtt tgcaccaaac cggccacgtg ctcgatgagc gttcgttccg tggcaacggc
     2641 aaaccaaggc tccgccgagg cccgggcgaa gacggcggcg ggatccggta gggcgccggc
     2701 aaagggactt ccttccaacc ggccggtgta tttcaagaat tcgcgctcca gttgtccgat
     2761 cgtcggaaaa ccgctttgcc gaacgagggc ctggatgtag ggccggtgca tgtcccggta
     2821 tccgtcggcg acgtcggcca gggccaacac cagggccagg cggacgtgcc gctccgtgag
     2881 gcccgcgatg tcttttaagg tctccttcgc tcccgcgccc agggcgctgt gggcccgaag
     2941 gaccagc
//
LOCUS       DAOQLS010000202         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19207_length_2940_cov_4.522704, whole genome shotgun sequence.
ACCESSION   DAOQLS010000202 DAOQLS010000000
VERSION     DAOQLS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19207_length_2940_cov_4.522704"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2438)
                     /locus_tag="PK362_04810"
     CDS             complement(<1..2438)
                     /locus_tag="PK362_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74189.1"
                     /translation="MTEERGKTSGIKRAAATVIALAFVFTSVGEPFAQTSFWAERQTS
                     RGQSAAAEDRRSSNLLAGLSWPSESLNPVLGAYRLPTALGSVVETHIAEGGKTGPTLL
                     HLQDAHGLYPAQINAAQTLAGLLRSGWWSGDAATLTVYQEGGAGPARVDWLSAFPFSD
                     IREKVAKAHLQKGEMTGEEYRSVVESSGTVRLIGVETDSLYEKNLAARHQTAAARTAA
                     DRYMTSVQARLALVKSKIYPESLKKLDTPLQEFAAQNISFVRYAQALLAVSPTGAPAE
                     TYPNLNAFHRLSRLESALDLRALSQERAELVEKIGQRAATTDVEGLAARALAYRQGRL
                     TAAEFYESLLTVADGLKRQKIDLPTRRLRDYVGYLKLSENLRHERLVAEVDQLKNRLL
                     ESFAKDDKVWRLVNLDRRVAQEKLLWRQEMTPDQYAAFRQEGALDWTDVESYLTTHGA
                     PASTPDLAWTKALPQVRDYYELALERDVALVKNTLADMEKTGARRAVLIAGGFHTPGL
                     TRLLRQSGASYAVVQPQFATDAPASDLSISQEPRAFSHTVREANQLVFREDLPLSRNL
                     PGQSLIEMFAKGSERVLSVPKSEAATNEQLLAAYREWLKSRGVDLELRDVAQLVDNAG
                     NKAQTLLFGEFNGQVFIIASDLASDQITWITEDTVRNGETANLNKRFSSLLQSRPGLQ
                     KLWTDVLSAFKSVAAARGGGNTAELREALNDRWAAFSSAGQIRQLAQTAVTATVLPRA
                     DVRRGETALATARQATASSSARVWDAVVRSWPTVRKWAVGALLFTAAPVAMAGEAAGL
                     SIGVSPAGILSALAG"
     gene            complement(2618..>2940)
                     /locus_tag="PK362_04815"
     CDS             complement(2618..>2940)
                     /locus_tag="PK362_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNC74190.1"
                     /translation="VISPYGAQVRMLRGFLPKGIEVGTVDGFQGREKEVVIVSLVRSN
                     EKAEVGFLSDTRRMNVAMTRARRLLIVIGDSATIGQHPFYAAFLDHVQAHGQHRSAWE
                     WLKQ"
BASE COUNT          534 a          841 c          964 g          601 t
ORIGIN      
        1 cccgccaacg cactgagaat tcccgccggc gaaacgccga tggagagccc cgccgcttcg
       61 cccgccatgg ccaccggcgc ggcggtaaac aggagggccc ccaccgccca tttgcgaacg
      121 gtcggccagg atcgcacgac ggcgtcccac acccgcgccg aggaggacgc ggtggcttgc
      181 cgggccgtgg ccagggcggt ttcgccccgc cgaacgtcgg cgcgggggag gaccgtggcc
      241 gtgacggccg tttgagccag ttggcggatt tggccggccg aggaaaaggc ggcccatcgg
      301 tcgttcaagg cttcccgcaa ctcggcggtg tttccgccgc cccgggcggc ggcgacggac
      361 ttaaaggcgg acaacacatc ggtccaaagt ttttgcaatc cggggcggga ttgcagcaag
      421 gaggaaaagc gtttattcag gttggccgtt tcgccgttcc gaacggtgtc ttcggtgatc
      481 caggtgattt ggtccgaggc caaatcgctg gcgatgatga acacttggcc gttgaactcg
      541 ccgaacagga gcgtttgcgc tttgttgccc gcgttgtcga ccaactgcgc cacgtcccgg
      601 agctcgagat ccactccccg ggatttcaac cattcccggt aggccgccaa caattgctcg
      661 ttcgtggccg cttcgctctt gggtacggac aaaacacgtt ccgatccctt ggcaaacatt
      721 tcgatgaggc tttggccggg gaggttccgc gacagcggga gatcttcccg gaaaaccaat
      781 tggttggctt cgcggaccgt atggctgaag gcgcggggtt cctggctgat ggaaaggtcg
      841 gacgccggag cgtccgtggc aaattgcggc tgaaccacgg cgtagctggc gccggattga
      901 cgaaggaggc gcgtcaggcc gggcgtgtgg aagccgccgg cgatcagcac cgcccgccgg
      961 gcgccggttt tctccatgtc ggccaaggtg ttcttcacca aagcgacgtc acgctccagg
     1021 gccaattcgt agtaatcgcg cacctggggc agagctttgg tccaggcgag gtccggggtc
     1081 gaggcgggcg cgccgtgcgt cgttagataa ctttccacgt cggtccaatc cagggcccct
     1141 tcctggcgga acgcggcgta ttggtccggg gtcatttcct ggcgccacag gagtttttcc
     1201 tgggccaccc gacgatctaa attcaccaat cgccaaacct tgtcgtcctt ggcgaaggat
     1261 tccagcaggc ggtttttgag ctgatcgacc tccgccacca ggcgttcgtg gcgcaggttt
     1321 tccgataatt tcaggtaacc gacataatcc cggaggcgac gggtgggcaa atcgattttt
     1381 tgtcgtttga gcccatcggc caccgttaaa agactttcat agaattcggc ggccgtcagg
     1441 cgcccctgtc ggtaggccaa ggcgcgggcg gccaggcctt ccacatcggt ggttgcggcc
     1501 cgctggccga ttttttccac caattcggcc cgttcctggg agagggcgcg caagtccaag
     1561 gccgattcca atcgggagag tcgatggaag gcgtttaagt tcggataggt ttccgccggg
     1621 gcgccggtgg gagagaccgc caggagagcc tgggcgtaac ggacaaagga aatattttgc
     1681 gcggcgaatt cttgaagggg ggtgtcgagc ttcttaaggg attccgggta aatctttgat
     1741 ttaacaagag cgaggcgggc ctgcacggag gtcatgtagc gatccgcggc ggtccgggcc
     1801 gcggcggttt ggtggcgggc ggccaggttc ttttcataaa ggctgtccgt ttcgacaccg
     1861 atcaggcgta cggtgcccga ggattccacg acgctgcggt attcctcccc ggtcatttcc
     1921 cccttctgca aatgggcctt ggcgactttt tcccgaatgt ccgaaaaggg gaaggccgac
     1981 agccagtcca cccgggccgg tcccgcgccg ccttcctggt agaccgtgag ggtggcggcg
     2041 tcgccgctcc accagccgga acgaaggagt cccgccagag tttgagcggc gttgatttgg
     2101 gccggataga gcccgtgggc gtcttgaaga tgaagaaggg tggggccggt tttgccgccc
     2161 tcggcgatgt gggtttcgac aacggatccc agggcggtgg gcagacgata ggcgcccaaa
     2221 acagggttta acgattcgct gggccaagac aggccagcca ggaggtttga ggaccggcga
     2281 tcctcggcgg ccgccgattg gccccgggag gtttggcgtt cggcccaaaa actggtttga
     2341 gcaaagggtt cgcccacgga ggtaaataca aacgcgaggg cgatgacggt cgccgccgct
     2401 ctttttattc cggaggtttt cccgcgctct tcggtcatat tcttgtcggc ctctcctctt
     2461 gtatagccgg ccggtgggtt ggccgtactt tcgtcattac tagttaacaa gaagcgtcag
     2521 aatttggcct taaattgttg taacggttgt gtaaattgat ggaatcatcg gtttgtcgtc
     2581 ggtaaaaatg ggggtgagtg ggaatttttc caacgtttta ctgcttgagc cactcccagg
     2641 cgcttcggtg ctgtccgtgg gcctggacat ggtccaaaaa ggcagcatag aaggggtgtt
     2701 ggccgatggt ggccgaatcc ccgatgacga tcaacagtcg ccgcgcccgg gtcatggcga
     2761 cgttcatccg ccgggtgtcc gacaggaacc ccacttcggc tttttcattg gaccgaacga
     2821 gggaaacgat gaccacttcc ttttcgcgcc cttgaaagcc gtctaccgtt cccacttcga
     2881 tccccttggg aagaaatccc cgcaacattc ggacctgggc gccgtaaggg gagatgacgg
//
LOCUS       DAOQLS010000203         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19231_length_2938_cov_4.500520, whole genome shotgun sequence.
ACCESSION   DAOQLS010000203 DAOQLS010000000
VERSION     DAOQLS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19231_length_2938_cov_4.500520"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PK362_04820"
     CDS             complement(<1..601)
                     /locus_tag="PK362_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC74191.1"
                     /translation="MDPLPGSPSINASDARPSTALDVLRGTFGFETFRPGQEKIINAV
                     LAGRDCIGVLPTGGGKSLTFQIPAKLLGGTVLVISPLISLMKDQVDALIQSGFRAAVI
                     NSTLDAEERRERMEGLRRGEYELVYLAPEALEEAARRFLAECPIRLLVVDEAHCISHW
                     GHEFRPSYRNLRGLKALLGNVPVLALTATATRAVARDILQ"
     gene            complement(609..2594)
                     /locus_tag="PK362_04825"
     CDS             complement(609..2594)
                     /locus_tag="PK362_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNC74192.1"
                     /translation="MNCRIVLIGLILATSHRGWGAADDIIVLPPVVIRAERDKDPVTK
                     REVTKEDLRLVPGTQGDPLKVLQSLPGVAVGSDGASDPAVRGTAPVDNSYYIDFQTVG
                     YLFHTGDLLSVLNGDLVDSFSIYPSAFGPEYNSALGAVIDVRLRDPRRDRWGSKLNLS
                     AFESDVLVEGPLGSRQAVYAAARRSYLDFLLSKFPGTSDIDVVQFPEFYDYQGKWIWE
                     NAAGHRLVFQTNGAWDKVALDIKSESDIAKRDPALAGRFAQTQDYHQEGLAWTWTGPS
                     VTNAVALSHTQTVLDNRVGGLGVVRINNRWWDVRDDVAWRLPAGNTLSLGVHHIYVPV
                     LLNVDVKVDFPSEFDPPPDFTSAPPRSEFGTLYINETLGYLKDRWSPGPDVTLSAGGR
                     WGRNGYLKENYGEPWAAGEWRFLENTTFSSAWGRYHQFPEGHQTTKNFGNPDLTALRA
                     EHVVAGLQQKMTDGWSVNLELYRKRLWNLPVEDPVLNFVNGARGLARGVEFLVKKERT
                     ARWAGWFAVSQSHSTRLNERTGQKVNFSQDQPWVLNLVATRRVDQFYSVGLRAQYHSG
                     APDTPITGTFIDGTGRVRPVYGPTGSVRLPDYHRIDLRFSRDFKMKGGTGNFYLDLIN
                     VLNHKNVAGYSYSDDYSGREKVTQLPFFPSVGVQLSL"
BASE COUNT          564 a          904 c          923 g          547 t
ORIGIN      
        1 gttgaaggat gtcccgggcc acggcccggg tggccgtggc ggtgagcgcc aggaccggga
       61 cgttgcccag gagggccttc aacccccgaa ggtttcggta ggacgggcgg aattcgtgcc
      121 cccaatggct gatgcagtgg gcttcgtcca ccaccagcag acggatggga cattccgcca
      181 agaaccgacg agccgcctct tccaacgcct ccggcgccag gtaaaccaat tcgtattctc
      241 cccggcgaag gccctccatt cgctcgcgac gttcctccgc atcgagcgtg gaattgatga
      301 ccgccgcccg gaaccccgac tggatcaaag cgtcgacctg atccttcatg agcgagatca
      361 aaggcgagat gaccaggacc gtgccgccca ggagtttggc gggaatttga aaggtcagtg
      421 attttccccc gccggtgggc agaacgccga tgcagtcccg cccggcgaga acggcgttga
      481 tgattttttc ctgccccggg cggaaggtct cgaaaccgaa ggtcccgcgc aacacgtcca
      541 gggccgtgga cggccgggcg tccgaagcgt ttatggaggg cgaaccggga agggggtcca
      601 tggaattcct ataaggacaa ctgaacgccg accgacggga agaaaggcag ttgggtgacc
      661 ttctcccggc cggaatagtc gtcggaatag gaataaccgg ccacgttctt gtggttgagc
      721 acgttgatta aatcgagata gaaattccct gtcccgcctt tcattttaaa atcccggctg
      781 aaccgcaaat cgattcgatg gtagtccggt aggcggacgg agcccgtggg gccgtagacg
      841 ggtcgcaccc ggcccgtccc atcgatgaac gtgccggtga tgggcgtgtc cggcgctccg
      901 ctgtgatact gggcccgaag gccgacggaa taaaattgat ccacccgacg ggtggccacg
      961 agattcagga cccagggttg gtcctgactg aaattgactt tctgccccgt ccgttcgttg
     1021 agccgcgtgg aatgggattg ggaaacggca aaccaaccgg cccatcgcgc cgtgcgctcc
     1081 tttttcacca agaactccac cccccgggcc agtccccggg cgccgttcac gaaattcagg
     1141 accggatcct ccaccggcag attccacagg cgttttcggt agagttccag gttcacggac
     1201 cacccgtccg tcattttttg ttgaagtccc gccaccacgt gttcggccct taaggccgtc
     1261 aggtccgggt tgccgaaatt ttttgtggtc tggtgtccct ccggaaattg gtggtagcgc
     1321 ccccacgccg aggaaaacgt cgtgttctcc aaaaaccgcc attcccccgc ggcccagggt
     1381 tccccgtaat tttcctttaa atagccgttt cggccccacc gcccgccggc cgacagcgtc
     1441 acgtccgggc cgggggacca gcggtccttc aggtacccca gggtttcatt gatgtaaagg
     1501 gtgccgaact cgctgcgcgg gggcgcgctg gtgaaatccg gcggggggtc gaattccgat
     1561 ggaaaatcga ctttcacgtc cacgttgagg agcacgggga cgtagatgtg gtgaacgccc
     1621 agggacaggg tgtttccggc cggaagccgc caggccacgt cgtcgcgcac gtcccaccat
     1681 cggttgttga tgcggaccac ccccaatccg cccacgcgat tgtccaggac ggtctgggtg
     1741 tgggagagcg ccaccgcgtt cgtcaccgac ggcccggtcc aggtccaggc gagtccctcc
     1801 tggtggtaat cctgggtttg ggcaaagcgc ccggccaaag ccgggtctcg cttggcgatg
     1861 tcggactcgc ttttaatgtc cagggccact ttgtcccagg cgccgttggt ttggaaaacc
     1921 agccggtgtc cggcggcgtt ttcccaaatc cattttccct gataatcgta aaactcgggg
     1981 aattgcacca cgtcgatgtc gctggtgccc gggaatttgg aaagcaggaa gtccaggtaa
     2041 ctccgccggg cggcggcgta cacggcctga cgagatccca gggggccctc caccaacacg
     2101 tcggactcaa aggcgcttaa attaagcttg ctcccccagc gatcccgacg ggggtcccgc
     2161 aggcgcacgt cgatcacggc ccccagggcg ctgttgtatt cgggcccgaa ggccgacggg
     2221 tagatggaaa aggaatccac gaggtcgccg ttcaacacgc tgagcagatc gccggtgtga
     2281 aacaaataac ccaccgtctg gaaatcgatg taatacgaat tatcgacggg cgccgtgccg
     2341 cgcaccgcgg gatcgctggc cccgtcggac ccgacggcca cgccgggcag ggattggagc
     2401 actttcaacg gatcgccctg ggttccggga accaatcgga ggtcctcttt cgtcacctcg
     2461 cgtttcgtca cgggatcttt gtcccgctcg gcacggatca ccaccggcgg aagaacgatg
     2521 atgtcgtccg ccgcgcccca cccccggtgg gaggtcgcca aaatcaaccc gatcaaaacg
     2581 attcgacaat tcatagggag gggccgccat cgcccgaagg actcattatt tgattttccc
     2641 gggatcgctt ggaagggccg cgggcggccg acttccccgg accgttttcg tcggccggtt
     2701 tcccccttgc cggggagccg tccgggggtt atataaagct gcaacggttt gacccaaacg
     2761 gaggtcggga cgggcatcgg ccccggagga ccctccggcg aagaaagacg cttcggcgac
     2821 gggttgatcc aggaggaaac gcatgacgcg gcaccatcga cgatggcaac aaggggtctt
     2881 ggccggggca acggtgtgca cggttttttg ttccgctctc ggggcggggc aggatctg
//
LOCUS       DAOQLS010000204         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19378_length_2925_cov_4.120557, whole genome shotgun sequence.
ACCESSION   DAOQLS010000204 DAOQLS010000000
VERSION     DAOQLS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19378_length_2925_cov_4.120557"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK362_04830"
     CDS             complement(<1..370)
                     /locus_tag="PK362_04830"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNC74193.1"
                     /translation="MSFATVAEALRDFRRGKPLVVVDDPGRENEGDVVVAASKATPAV
                     INFMAREARGLICVPMLGDRLDALKLNAMVEHGAPREAAFTVSVDAKKNVTTGISAHD
                     RARTVAALIHPSTGPEDLSRP"
     gene            complement(367..960)
                     /locus_tag="PK362_04835"
     CDS             complement(367..960)
                     /locus_tag="PK362_04835"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNC74194.1"
                     /translation="MFTGIIAARGRVLRLDGLRLEVEGPLAKIRVGDSVAVNGVCLTV
                     VKSTGTARARRMAFDLSLETLRRTSLGVWKAGRAVNLETALRAGDPLGGHFVQGHVDG
                     IGKLVSRRPEKGGVFYVFEAPGELARYLVRKGSVAVDGISLTVVNPRKGRFDVAVIPH
                     TERVTNLGSLRIGDPVNIEADMMAKHIEQLTSAWRRP"
     gene            complement(960..2012)
                     /gene="ribD"
                     /locus_tag="PK362_04840"
     CDS             complement(960..2012)
                     /gene="ribD"
                     /locus_tag="PK362_04840"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNC74195.1"
                     /translation="MIRALNLAERGRLNAHPNPLVGCVVARGPRVLAEGWHDRFGGDH
                     AEVAALRRAGSRARGATLYVTLEPCSRWGKTPPCTDAVKAAGVRRVVIGARDPSLRGG
                     GVAVLKRAGLRVDSGLHQREVLRQNAAFFKKVKTGLPYVVVKMAQTLDGKTASRTGGS
                     RWITGVRARSLGHRLRAQSDAVMVGGETVRRDNPGLTSHGRGPDPLRVVLSSSLQFSP
                     RAAVFAQGAPTWVLTGDSSPAARKKSLERRGAQILSFRSTGGRIRLKEAFRALARRGV
                     SRILVEGGGRTAAAVLEEKLADEAYVFLAPSFLGGVGAPTGVAGRGWPRPAAGPRLKD
                     VGVNRLGDDVLIHGYF"
     gene            complement(2186..>2925)
                     /locus_tag="PK362_04845"
     CDS             complement(2186..>2925)
                     /locus_tag="PK362_04845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNC74196.1"
                     /translation="QVFVNLIDNACDAMSTTEGEKRIHITARAEGGAVCVDVADTGPG
                     VPDAVRDRLFEPFFTTKAEGRGTGLGLPVCRQIVEDHGGRIQFTTELGKGTVFHFQIP
                     AATEEAALAPATAPRLPPVKNKRVLIVDDEPDVLGFLAKVVQAEGNKVEFAGTLKDAI
                     ARASQGPFDLVVTDIRLGEGTGLSLYENWTLWSGKPRPVFVFMTGDVVNSTLAQEMEK
                     KGVHILHKPIDMTSFQTTLRTVLATPR"
BASE COUNT          523 a          984 c          954 g          464 t
ORIGIN      
        1 cggggcggga caggtcttcc gggccggtgg acgggtggat caaggccgcc acggttcggg
       61 cccggtcgtg ggcggagatc cccgtggtca cgtttttctt ggcgtcgacc gacacggtga
      121 acgccgcctc ccggggagcc ccgtgctcca ccatggcgtt cagtttcagc gcgtccaggc
      181 ggtcccccaa catggggacg caaatcaacc cccgggcttc ccgggccatg aaattaatga
      241 cggcgggcgt ggccttggag gcggccacca ccacgtcgcc ttcgttttcc cggccggggt
      301 cgtccaccac caccaggggt ttgccccggc gaaaatcccg cagggcctcg gccacggtcg
      361 cgaaactcac ggccgccgcc aggccgacgt caattgttca atgtgtttgg ccatcatgtc
      421 cgcctcgatg ttcacgggat ccccgatgcg gagggaaccc aaattggtca cgcgctccgt
      481 gtgcgggatc accgccacgt cgaaccggcc cttccggggg ttcaccaccg tcaagctgat
      541 gccgtccacc gccaccgacc cttttcgaac cagataccgg gcgagttccc ccggcgcttc
      601 aaagacgtaa aaaacgccgc ctttttccgg ccggcgggac accaactttc cgatgccgtc
      661 cacgtgcccc tggacgaaat gcccccccag gggatcgccc gcccgaaggg ccgtttccaa
      721 attgacggcg cgccccgcct tccaaacacc gaggctcgtc cgccgaaggg tttccaggga
      781 caaatcaaag gccattcggc gggcgcgggc cgttcccgtg gatttaacaa cggtgaggca
      841 gacgccgttg accgccacgg aatcgcccac ccggattttg gccagggggc cttcgacttc
      901 cagccggagc ccgtccaatc ggagcacccg accccgggcc gcgatgatgc cggtgaacat
      961 cagaaatacc cgtggatcaa aacgtcgtcg cccagccggt tcaccccgac gtcctttaaa
     1021 cggggacccg cggcgggacg gggccatccc cggccggcca cgcccgtggg cgccccgacg
     1081 ccgcccaaaa aggaaggcgc caaaaacaca taggcttcgt cggccagttt ttcctcgaga
     1141 acggccgccg ccgtgcggcc gccgccctcc accagaatcc gggaaacgcc ccgccgggcc
     1201 agggcccgaa aggcttcttt gagccggatc cggccgccgg tggaacggaa ggacaaaatc
     1261 tgggcgcccc gccgctccaa ggattttttt cgggcggccg gggacgaatc cccggtcagc
     1321 acccaggtgg gggcgccttg ggcgaaaacc gccgcccggg gcgaaaattg gagggaggag
     1381 gacaagacca cacgcagggg atcgggtccc cggccatggc tcgtgagacc ggggttgtcc
     1441 cgtcggacgg tttcgccgcc caccatgacc gcgtccgact gggcccgcag gcggtggccc
     1501 agggaccggg cccggacgcc cgtgatccac cgggagcctc cggtgcggga cgcggttttc
     1561 ccgtccaggg tctgggccat tttgaccaca acgtagggca ggccggtttt gaccttttta
     1621 aaaaaagcgg cgttttgccg aaggacttcc cgctggtgaa gccccgaatc cacgcgaagg
     1681 ccggcgcgtt ttaaaacggc cacgccgccg ccccgaaggc tcgggtcccg ggcgccgatc
     1741 acgacccgcc gaacccccgc ggcttttacg gcgtccgtgc agggcggggt tttgccccat
     1801 cgggagcagg gctccaaagt gacgtacagc gtcgctcccc gggcgcggga acccgcccga
     1861 cgcagggcgg ccacttccgc gtggtcgccg ccgaagcgat cgtgccaacc ttcggccaaa
     1921 acgcgggggc cccgggcgac gacgcagccg accaggggat tggggtgggc gttcaagcgg
     1981 ccgcgttcgg ccaaattgag cgcgcgaatc atgtggaggg aatcttcggg acgaaacacg
     2041 gccgggggtc taacgggaaa gcgaaaaaag aaaggatcga cgtcgcataa aacacccggc
     2101 ctccggcgag ggaggggcct ttattggaat ccgcgtattt tagcttttcc gagggaagga
     2161 aatcaacgac cggcgagagg aaacttcagc ggggcgtggc caggacggtg cgcagggtgg
     2221 tttgaaaaga cgtcatgtcg atgggcttgt ggaggatgtg gacgcccttt ttctccattt
     2281 cctgggccag ggtcgagttg accacgtcgc ccgtcataaa aacgaagacc ggccggggct
     2341 tgccggacca caacgtccaa ttctcgtaaa gcgacaggcc ggtgccttcg cccagccgaa
     2401 tgtccgtcac caccaagtcg aagggcccct gggaggcccg ggcgatggcg tctttcaagg
     2461 tgccggcgaa ttccaccttg ttgccctcgg cctgaaccac cttggccaga aacccgagca
     2521 cgtcgggttc gtcgtccacg atgagcacgc gcttgttctt gacgggcggc aggcgcgggg
     2581 ccgtggccgg cgccaaggcg gcctcctcgg tggcggcggg aatttgaaaa tggaagacgg
     2641 tccccttgcc gagctcggtg gtgaattgga tccgcccccc gtggtcctcg acgatttggc
     2701 ggcacaccgg caatcccagt cccgtgccgc ggccttcggc tttcgtggtg aaaaacggct
     2761 caaacaaccg gtcccggacc gcgtccggca cgccggggcc ggtgtccgcg acatccacgc
     2821 aaaccgcgcc gccctcggcc cgcgcggtga tatgaattct cttctcgccc tcggtggtcg
     2881 acatggcgtc gcaggcgttg tcgatcaggt tcacgaacac ctgct
//
LOCUS       DAOQLS010000205         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19588_length_2907_cov_3.730715, whole genome shotgun sequence.
ACCESSION   DAOQLS010000205 DAOQLS010000000
VERSION     DAOQLS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19588_length_2907_cov_3.730715"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PK362_04850"
     CDS             <1..1504
                     /locus_tag="PK362_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HNC74197.1"
                     /translation="MLFLFAQLGLVFHLAVSFALVWADDEERFWQMNRVLFLRFIATG
                     VFSNTIFAGVALCLGSLKLLFKWEIPGEIYLDLWYFCVFIFNTLFFCAGVPTKDSLPR
                     LQGDYPRLLKIFCQYVLVPLLAVYGVILYAYLARVIVHWEIPKGQAAALISGFSIAGM
                     LTNLLLHPLAEKPENRWMRVFGTGFYGGLPPLLGLMAVALYQRIAPYALTENRYILCL
                     LCLWLFGVAVYFLATRSRRIKVVPVSLACLALLTAFGPLGVRAVAERSQLKRLLALLK
                     TNGRLGPDGVIQKADKDLTFEDGKEASGIFDYLLSRHGWGRLKAHLPAAVVASVGEKE
                     MKKIADEKAPSGAAVMQALGMAYHNRWESPTNEDSLNFYGRSDNGGAALDVRGFEGAL
                     LNVEWNLSGAKPSAEGWSAGPTGPGAGRVTLFDRGREVWSLDLLDYAAKHRESFTEKG
                     NPKMRPVTIGPVDGAIRAVFVVETLNATLSPDRPRVTYLKGHLFWSRSKS"
     gene            1675..>2907
                     /locus_tag="PK362_04855"
     CDS             1675..>2907
                     /locus_tag="PK362_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74198.1"
                     /translation="MSPKDSAAAGYADLRARAEKFYGEKSFRQAHELYEQAADLSLSP
                     AEKIWVTFRRADTAWREESSGGTPDNQRVQKSRETLQSLVNDKDFGHWPEATRAWINE
                     SLGDSYWEPVNSRNWYMGWSFYQKAFDLWAGSTDLETARREYLRLAWKAMEPPSAPEY
                     YTYGYYGNWLPVAVARNVARLAVAPEDRARAEYLLALSLRQEGARSALVIEAFEKSLA
                     AAKTAYYDDALYALAETYENGGRRVRRDGRWEEQPDYAKALALYQRLTKEFKKSESRY
                     WDQAQESAKNITEPQLNISVGQMFLPDRRPSFYLSWRNVSDVKFRLEKVDPLTDFAPQ
                     PGDSASNWTEGFRKGGKKIRQWEKTLVAPEHAPGNENVTLEEPLPAGTYLLTAESGKL
                     KSTEIVLMTDALVLTRSYP"
BASE COUNT          528 a          916 c          880 g          583 t
ORIGIN      
        1 catgctcttt ttgttcgccc agctgggatt ggtgttccat ttggccgtct ccttcgccct
       61 ggtgtgggcc gacgacgagg aacgcttctg gcaaatgaac cgggtgctct ttctgcgttt
      121 catcgccacg ggcgtttttt ccaacaccat tttcgcgggg gtggccctgt gcctggggtc
      181 cttgaaactt ctgttcaaat gggaaatccc cggcgaaatt tatttggacc tgtggtactt
      241 ctgcgttttc attttcaaca ccctgttctt ctgcgcgggg gttccgacca aggactccct
      301 tccgcgcctc cagggcgact acccgagact tctcaaaata ttttgccagt acgtgctggt
      361 gccgctcttg gcggtctacg gggtcatcct ctacgcctat ctggcccggg tgatcgtcca
      421 ttgggaaatt cccaaggggc aggcggcggc cttgatctcc ggcttttcca tcgcggggat
      481 gttgaccaac cttctcctgc accccctggc ggaaaagccg gaaaaccgct ggatgcgggt
      541 tttcggcacg ggcttctacg ggggccttcc gccgcttttg gggctcatgg ccgtggccct
      601 ttaccagcga atcgcgccct acgctctgac ggaaaaccgc tacatcctct gcctcctttg
      661 cctttggctt ttcggggtgg cggtgtattt cctggcgacc cggtcgcggc ggatcaaggt
      721 cgtgcccgtt tccctggcct gtttggccct gctcacggcc ttcgggcccc tgggcgtgcg
      781 ggcggtggcc gagcgcagtc agttgaaacg cctgctcgcc cttcttaaaa cgaacggtcg
      841 cctggggccc gacggcgtga ttcaaaaggc ggacaaggat ctgaccttcg aggacgggaa
      901 agaagcctcg ggcattttcg attatctgtt gtcgcgccac ggttgggggc ggctcaaagc
      961 ccacctgccg gcggccgtgg tggcctcggt gggggaaaaa gaaatgaaga agatcgccga
     1021 tgagaaggcc ccctcgggcg cggcggtcat gcaggctctg ggaatggcct accacaaccg
     1081 ttgggaatcc ccgacgaacg aggattccct caacttttac ggccgaagcg acaacggcgg
     1141 cgcggccctg gacgtccggg gtttcgaggg ggcgcttttg aacgtggagt ggaacctgtc
     1201 cggcgccaag ccgagcgccg agggttggag cgcgggcccc accggaccgg gcgccgggcg
     1261 cgtgaccctg ttcgaccggg gccgggaggt ctggagcctg gacctgctgg actacgcggc
     1321 caaacaccgg gagtccttca ccgaaaaagg caaccccaaa atgcgcccgg tgaccatcgg
     1381 ccccgtcgac ggggccattc gcgcggtctt cgtggtggaa accctgaacg ccaccctttc
     1441 cccggaccgt ccccgcgtca cctatttaaa gggccatttg ttttggtccc ggtccaaatc
     1501 ctgaattaaa tcaatccccc ggggtgtggc gccgggggcg tccgtccact atactttcag
     1561 gatacaagcc catgccacaa tttcccggga acaaattcct tttttcggtg tcggagggtt
     1621 atgatgaaac catcccgcgt tgtcctttcc atactcttat tgggggtcgc ttccatgtcg
     1681 cctaaagatt ccgccgccgc cggttacgcc gatcttcgcg cccgggccga aaaattttac
     1741 ggggagaaat ccttccgcca ggcccacgaa ctctacgaac aggcggcgga cctttccctg
     1801 tccccggcgg aaaagatctg ggtgaccttc cgccgggccg acaccgcctg gcgcgaggaa
     1861 tcctccggcg gtacgccgga caaccagcgg gttcagaaat cccgggaaac ccttcaatcc
     1921 ctggtgaacg acaaggactt tggtcattgg cccgaggcca ctcgggcctg gatcaacgaa
     1981 agcctgggcg attcctattg ggagccggtc aattcccgca attggtacat ggggtggagt
     2041 ttttaccaga aggcctttga cctttgggcg ggttcgaccg acctggaaac ggcccgccgg
     2101 gagtacctgc gcctggcctg gaaggccatg gagcccccgt cggcgccgga gtactacacc
     2161 tacggctatt acggcaactg gctgccggtg gcggtggcgc ggaacgtcgc ccgcctggcc
     2221 gtggcgcccg aggaccgggc ccgggcggaa tatcttctgg ccctgtccct ccggcaggag
     2281 ggggctcgct ccgccctggt gatcgaggcc tttgaaaaat ccctggccgc cgccaagacg
     2341 gcctattacg acgacgccct ctatgccctg gccgaaacct acgaaaacgg cgggcgccgc
     2401 gtccgccggg acggccgctg ggaggagcag ccggactacg ccaaggccct ggctctctat
     2461 cagcgcctca cgaaggaatt caaaaaatcc gaaagccgct attgggacca ggcccaggaa
     2521 agcgccaaga acatcaccga gccccagttg aacatcagcg tgggccagat gtttttgccc
     2581 gatcgccggc cgagctttta cctctcctgg cgcaacgtgt ccgacgtcaa attccggttg
     2641 gaaaaagtcg accccctgac ggacttcgct ccccaacccg gggattcggc ctccaattgg
     2701 acggaagggt tccgcaaagg cggtaaaaaa atccgccaat gggaaaaaac gttggtcgcg
     2761 ccggaacacg cccccggcaa cgaaaacgtc actttggaag aacccctgcc ggccgggacc
     2821 tatttgctca cggcggagag cggcaagttg aaatccaccg aaatcgttct tatgaccgac
     2881 gcgctggtgc tcacgcggtc ctaccct
//
LOCUS       DAOQLS010000206         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19601_length_2906_cov_4.411785, whole genome shotgun sequence.
ACCESSION   DAOQLS010000206 DAOQLS010000000
VERSION     DAOQLS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19601_length_2906_cov_4.411785"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1067
                     /gene="argC"
                     /locus_tag="PK362_04860"
     CDS             45..1067
                     /gene="argC"
                     /locus_tag="PK362_04860"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNC74199.1"
                     /translation="MISAAVVGATGYTGGELLRILLQHPEVRVVRVTSESKPGRPLAE
                     VHPFLKGRLSLTLEKFNARAIARDVDVAFFALPHGVGSKNIADFVREGKKAVDLSADF
                     RIRRLPVYESTYKVKHASPALLAKAVYGLPELFREKIPGAPVVANPGCYATTTILALA
                     PLLKNKMVRPGSIVVDAKSGVSGAGKKLDPMYLFCEAAENFQAYAVVKHRHVPEIEQA
                     LGDHAGAAPTLTFVPHLVPMVRGIHVTAYADLKKKTTAARLRAVYEAAYAGERFIRVL
                     PEGQWPQTKDVAGTNYVDIAVAVDERHGRAVVLAVLDNLVKGAAGQAVQNMNLLFGLD
                     EAAGLK"
     gene            1111..1323
                     /locus_tag="PK362_04865"
     CDS             1111..1323
                     /locus_tag="PK362_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNC74200.1"
                     /translation="MGFIGFLKKLLGMTNAGDQRLLCAECGGDFVFEAGEQAFFAERG
                     LTPPKRCPDCRKKNRGRRRFGRGRGR"
     gene            1320..2528
                     /gene="argJ"
                     /locus_tag="PK362_04870"
     CDS             1320..2528
                     /gene="argJ"
                     /locus_tag="PK362_04870"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNC74201.1"
                     /translation="MKALPLSNADLPPGFALHGLRAGISKKAGKKDLALFLSERPARA
                     AGVFTTNLVKAAPVRYCQKKLAASRSARAVVINSGCANACTGRPGAADTAWTAATLGR
                     GLGFKPSDVLVASTGVIGRPLPRPALGRGLRDLIGRVEKKQAGQTADAVRAIMTTDTR
                     PKAARATFNIGGQTRVVWGCAKGAGMIHPNMATMLSVVLTDAGVPAPALQRALAAAAR
                     GTFNCVSVDGDTSTNDSLFLLDNGAPRSYSPKELSAFQSALEEVCLSLAGQIAADGEG
                     ATRVAWIFVQGARTEKAAHQLAATVATSALFKTALHGADPNWGRVLAALGRAGVPFDP
                     DRVDVRIGDTLVCRAGGRAPHSEKAVHQALKKDRVTVTVDLHQGKAWSHYVTCDYSKD
                     YISINADYTT"
     gene            2556..>2906
                     /locus_tag="PK362_04875"
     CDS             2556..>2906
                     /locus_tag="PK362_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNC74202.1"
                     /translation="MKTRTFRLQKGALVPARIAETVEILRSGGVAVFPTDTVYGLGAS
                     VFRPTAIKKIYALKGRSYKKPLPFLVAGLEQALALVEPPDARLRKVLKKYWPGPLTVV
                     FNTSPLGRWTTGGKD"
BASE COUNT          506 a          964 c          956 g          480 t
ORIGIN      
        1 cccgctgaaa cggcggcgga cgagtcggac gaggaggaat cggaatgatc tcggcggcgg
       61 tggtgggcgc gacgggttac acgggcgggg agcttcttcg aattttgctt caacatccgg
      121 aggtccgtgt cgtccgggtc acctccgaat ccaagcccgg ccgccccctg gccgaagttc
      181 atcccttcct aaaagggcgg ctgagcctga ccctcgaaaa attcaacgcc cgggccatcg
      241 cccgggacgt cgacgtggcc ttcttcgccc tcccccacgg cgtcggttcc aaaaacatcg
      301 ccgatttcgt ccgggaaggc aaaaaagccg tggacctgtc ggcggatttt cgaattcgcc
      361 gcctcccggt ttacgaatcg acctacaagg tcaaacacgc gtcgccggcc cttcttgcca
      421 aagccgtcta cggtctcccg gagctgttcc gggagaagat cccgggcgcc ccggtcgtcg
      481 cgaaccccgg ctgttacgcc acgacgacca ttctggccct ggcgcccctg ttgaaaaaca
      541 aaatggtccg cccgggctcc atcgtcgtgg acgccaagtc cggcgtctcc ggcgcgggga
      601 aaaaactgga ccccatgtac ctcttttgcg aagccgcgga gaatttccag gcctacgccg
      661 tggtcaagca ccgccacgtg ccggaaatcg agcaggccct gggcgaccac gcgggggcgg
      721 cgcccaccct caccttcgtg ccgcatctgg tgcccatggt ccggggcatc cacgtgacgg
      781 cctacgccga tttgaaaaaa aagacaaccg ccgcgcgcct gcgcgcggta tacgaagcgg
      841 cctacgcggg ggagcggttc atccgcgttc ttcccgaggg gcaatggccg cagaccaagg
      901 acgtggcggg gaccaattac gtcgacatcg cggtcgccgt ggacgaacgc cacggtcgcg
      961 cggtggtgct cgccgtgctg gacaacctgg tcaagggcgc ggccggccag gccgtgcaga
     1021 acatgaacct gctcttcggt ttggatgagg ccgcggggct caaataacta aaacgcggac
     1081 cggcgcccgg cgcccccgtg ggaggacacg atgggtttca ttgggttttt gaagaagttg
     1141 ttgggcatga ccaacgcggg cgaccagcgg ttgttgtgcg ccgaatgcgg gggggatttc
     1201 gttttcgaag cgggcgagca ggcctttttc gccgagcggg ggttgacccc gcccaaacgg
     1261 tgccccgatt gccgtaagaa aaaccgcggc cgccgccgct ttggacgggg ccggggccgt
     1321 tgaaagccct ccccctttcg aacgccgatc tcccgccggg cttcgccctg cacggcctgc
     1381 gggccggcat ttccaaaaag gcgggcaaaa aagacctggc gctgttcctc tcggaacggc
     1441 ccgcccgggc ggcgggggtt ttcacgacca atctcgtcaa agccgcgccg gtccgctatt
     1501 gccagaagaa attggcggcc tcccggtcgg cgcgggccgt ggtgatcaat tccggctgcg
     1561 ccaacgcctg cacgggccgc cccggcgcgg cggacaccgc ctggaccgcc gccaccctgg
     1621 ggcgggggtt ggggttcaag ccgtcggacg ttctggtcgc ctcgaccggc gttatcggcc
     1681 gccccttgcc gcgtccggcc ctgggccgcg gccttcggga cctgatcggc cgggtggaaa
     1741 aaaaacaggc gggtcaaacg gccgacgccg tccgcgccat catgaccacg gacacccggc
     1801 ccaaagccgc ccgggcgacg tttaacatcg gcgggcaaac ccgggttgtg tggggatgcg
     1861 ccaagggcgc cggcatgatc caccccaaca tggccacgat gctttcggtc gtgctgaccg
     1921 acgcgggggt tccggccccg gcgctccagc gcgccctggc ggccgccgcc cgggggacct
     1981 tcaattgcgt gagcgtcgac ggagacacct ccaccaacga cagcttgttc ttgttggaca
     2041 acggagcccc gcggtcttat tccccgaagg aattgtccgc tttccaatcg gccctggagg
     2101 aagtgtgtct ttccctggcg ggccagatcg ccgccgacgg ggaaggcgcc acgcgggtgg
     2161 cgtggatctt cgtccaaggc gcccggaccg aaaaggcggc ccatcaattg gccgcgacgg
     2221 tcgccacctc cgccttgttt aagaccgcgc ttcacggcgc cgatcccaac tgggggcggg
     2281 tgttggccgc cctgggccgg gccggcgtgc ccttcgaccc ggaccgggtg gacgtgcgca
     2341 tcggcgacac gctggtttgc cgcgccggcg ggcgggcgcc ccattcggaa aaggcggtcc
     2401 accaagccct gaaaaaagac cgggtcacgg tcacggtcga cctccatcaa gggaaggcct
     2461 ggagtcacta cgtgacctgc gactattcaa aagattatat ttcgatcaac gccgattaca
     2521 cgacctgacg tcggttcttt agaacggggg aatcggtgaa aacccggacc ttccgccttc
     2581 aaaaaggggc cctggtcccc gcccggatcg ccgagaccgt cgaaattctt cgatccggcg
     2641 gggtggcggt ttttccgacg gacaccgtgt acgggctggg ggcgagcgtt ttccgcccga
     2701 cggcgatcaa aaagatctac gccttgaagg gccggtccta taaaaagccc ctgcccttcc
     2761 tggtggccgg tctcgagcag gcgctggccc tggtggagcc ccccgacgcc cggctccgaa
     2821 aggtgctcaa aaaatattgg ccggggccgt tgacggtggt gttcaacacc tcgcccctgg
     2881 gccgctggac gacgggggga aaggac
//
LOCUS       DAOQLS010000207         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19780_length_2891_cov_3.448519, whole genome shotgun sequence.
ACCESSION   DAOQLS010000207 DAOQLS010000000
VERSION     DAOQLS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19780_length_2891_cov_3.448519"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2891
                     /locus_tag="PK362_04880"
     CDS             <1..>2891
                     /locus_tag="PK362_04880"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNC74203.1"
                     /translation="VMTFTADVTDEALDVTFPRAVVDMAMISGIEAQVVSVSDDNFLG
                     FVERKLFWLFWNETNPLTGLVSDRVDNWRPLTGGAASIASTGFSLSALTVAAQRGWVP
                     RDQALQRVKNTLATFAGSSFAQPGQTVFLPHLQGFWYHWIDRDTGARAWDSEVSTVDS
                     ALLLMGALEAKEYFKTDATVGAMVDAIQNRMNWSWWLNRVPAGQGDPAWEGQFVTMAW
                     VPEVRNDPHYYPADPAIGGSFTRARWNGYSESVFVNLLAMGAPTSAISTAAWTNMGRS
                     WVDQYGYTNLHQAPLFEHQYHHLFYDLRNKHDAFADYGETTRRATLANKNYCQTVPGY
                     AADRWGVTSADNPANDEYKFDYGVPPGGNADGTVAPTAALSSIVFTPTESIRAARHMY
                     FQYKHDIWGRYGFTDSFNVNAQARSKSALSLDNGAMLLAIENYRSGLIRNTFARTATA
                     TRGLARTGFSAYDGKPYYPASSYIAGNLPAYAFDGNPATRWESEWSDNQWLAVDYTTP
                     RLVNRARLTWETAHPAQERIEYSSDGNTWVAASATVNSDGGDDTITFPAVRARFFRVK
                     GLARATPWGNSLFECVFDYDANLQQSTYFGGTGNSVINDVVTDVNGNVYVAGATTYGH
                     PTTAGAYGATYSGGTWDAFVSKFSPSGQLLYSTYLGGSAEDSAAALAVDAQGIVTVTG
                     LTYSANFPTTANAVDRTLGGERDAFVARLSASGGSLLYSTYLGGNSWDYGQQIALGPT
                     GDIFMAGFTHGDFPTTPGAAQTVFGGYGDAYVVHLSTGGALLYSTYLGGQSWDGPGGI
                     AVDAQGSAVVAGNTHSIDFPTTVGAYDRACDNCATNYSTDGFVAKISPTGAGLVFSTL
                     LGGAAAPSSEGLNGVALDAQGRVAVVGVSNAADFPTTSNAYRRTASGGYDVVAALLSA
                     DGSQLLYGTYLGGAGNDRAMDMVTDPQGRIYVSGYTDSVNFPTTSGAPQT"
BASE COUNT          517 a          991 c          863 g          520 t
ORIGIN      
        1 gtgatgacct tcaccgccga cgtcaccgat gaagcgctgg acgtcacgtt cccccgggcg
       61 gttgtggaca tggccatgat ttccggcatc gaggcccagg tggtctcggt ctccgacgac
      121 aacttcctcg gattcgtgga gcgaaaactt ttttggcttt tctggaacga aacgaatccc
      181 ttgacgggct tggtgagcga ccgcgtggac aactggcgcc ccttgaccgg cggggcggcc
      241 agcatcgcct ccaccggatt ttctttgagc gccttgaccg tggcggccca acggggctgg
      301 gtgccccggg accaggctct ccagcgggtc aaaaacaccc tggcgacctt cgccgggtcc
      361 tccttcgccc aaccggggca aaccgttttt cttccccatt tgcagggctt ctggtaccac
      421 tggatcgacc gggacaccgg cgcccgcgcc tgggattcgg aagtctcgac ggtggactcc
      481 gccctccttc tgatgggcgc cctggaagcc aaggaatact tcaaaaccga cgccaccgtc
      541 ggcgccatgg tggatgccat tcaaaaccgg atgaattggt cctggtggct gaaccgggtg
      601 ccggcggggc agggcgaccc ggcctgggaa ggccaattcg tcaccatggc ctgggtcccc
      661 gaagttcgca acgaccccca ctattatccc gcggatcccg ccatcggcgg ttcctttacc
      721 cgggcccgat ggaacggcta ttcggaaagc gtcttcgtca atctcttggc catgggcgcg
      781 ccgaccagcg ccatcagcac ggcggcctgg accaacatgg gtcgctcctg ggtcgatcaa
      841 tacggttaca ccaaccttca ccaggcgccc ctttttgaac accaatatca ccacctgttc
      901 tacgatctac ggaacaaaca cgacgccttt gccgattacg gggaaaccac ccgtcgcgcc
      961 accctggcca ataaaaacta ttgccaaacc gtcccgggat acgccgccga ccgctggggc
     1021 gtcacctcgg cggacaatcc cgccaacgat gagtataaat tcgattacgg cgttcccccg
     1081 ggcggcaacg ccgacggcac cgtggcgccc acggcggccc tttcgtccat cgtgttcacg
     1141 cccacggaat ccatccgggc ggcccgccac atgtatttcc aatacaaaca cgacatctgg
     1201 ggtcgctacg ggttcacgga ttccttcaac gtgaacgccc aggcccgttc caagagcgcc
     1261 ctcagtctgg acaacggggc catgctgttg gccatcgaaa attatcgatc gggattgatt
     1321 cgcaacacct tcgcccgcac ggcgaccgcc acccgggggc tggcccggac gggtttttcc
     1381 gcctacgacg gcaaacccta ttaccccgcc tcctcctaca tcgcggggaa tcttccggcc
     1441 tacgccttcg acggcaatcc ggccacccgc tgggaatccg aatggtcgga caaccaatgg
     1501 ctcgcggtgg actacacgac cccgcgcctg gtgaaccggg cgcgcctcac ctgggaaacg
     1561 gcccatccgg cccaggaacg catcgagtac tcgtcggacg ggaacacctg ggtcgcggcc
     1621 agcgcgacgg tcaattccga cgggggagac gacaccatca ctttcccggc ggtccgcgcc
     1681 cgatttttcc gcgtgaaggg cctcgcccgg gcgaccccct ggggcaattc cctctttgag
     1741 tgcgtctttg actacgacgc gaatcttcaa caaagcacct acttcggggg caccggcaac
     1801 tccgtcatca acgacgtggt gaccgacgtg aacggcaacg tgtacgtggc cggcgccaca
     1861 acctacggcc atcccacgac cgcgggggcc tacggcgcca cttacagcgg agggacctgg
     1921 gacgccttcg tgtccaaatt cagccccagc ggccagcttc tctacagcac ctacctcggt
     1981 ggatccgcgg aagattccgc cgccgccctc gcggtcgacg cccagggaat cgtgaccgtg
     2041 acgggattga cctattccgc caatttcccg accacggcca acgccgtgga ccggaccctc
     2101 ggcggagaac gggacgcctt cgtggcccgc ttgagcgctt cgggcggttc ccttctttat
     2161 tcgacttatc tggggggcaa ttcctgggat tacgggcagc aaatcgccct gggcccgacc
     2221 ggggacattt tcatggcggg ctttacgcac ggagactttc cgacgacgcc cggcgcggcc
     2281 cagaccgtct tcggcggcta cggcgacgcc tacgtggttc atctgtccac cggcggcgct
     2341 cttctctaca gcacctatct cggcggccaa agctgggacg ggccgggggg cattgccgtc
     2401 gacgcccaag ggagcgccgt ggtggcgggg aacacgcact cgattgattt ccccaccacg
     2461 gtgggggcgt acgatcgggc gtgcgacaac tgcgccacca actacagcac cgacgggttt
     2521 gtggccaaaa tatcgccgac cggcgccggt ctggtctttt cgaccctgtt gggcggcgcg
     2581 gcggctccgt cgagcgaggg gttgaacggc gtcgccttgg atgcccaggg tcgcgtggcg
     2641 gtggtcggcg tcagcaacgc cgccgacttt ccgaccacgt ccaacgccta tcgtcgaacg
     2701 gcctcggggg gctacgacgt cgtggcggcc ctgttgagcg ccgacggatc ccaattgctc
     2761 tacggcacct acttgggcgg ggccggcaac gaccgcgcca tggatatggt gaccgatccc
     2821 caggggagga tttacgtctc cggttatacg gattcggtga atttccccac aacgtccggc
     2881 gccccgcaaa c
//
LOCUS       DAOQLS010000208         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19818_length_2887_cov_4.043785, whole genome shotgun sequence.
ACCESSION   DAOQLS010000208 DAOQLS010000000
VERSION     DAOQLS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19818_length_2887_cov_4.043785"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PK362_04885"
     CDS             <1..1124
                     /locus_tag="PK362_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74204.1"
                     /translation="PSTAGIGLPNPSLRQRLLNFIFGEKLKTESVSPNVSAEGPPLSK
                     PDSAHAVIDDLDNSLGYIDGKPMPTPDHLKTFNPGETLPSGDKAGKGPGAASLSEYIE
                     TTLTKSEVISQGRLGFDPAFESKGAAESGMVGLSKSGLEAFRVLREAGIRVSVGSEAA
                     WGELNAAGSHFINEVFNSPTRTNKLTTTSDGTRFLDITDNKGRTVRATEKGDFVGFIV
                     DRPEALPQWNPKALPPSRAGPQSKTNFQILVSRNRVKYTPINPGPLPKEIASTFRSAT
                     YEAVKLDQPAELYRVFSDPSKKMGPYWTIKKPESSLKAIVDNALDPNWGNRATDIVSI
                     RVPAGETIYVGPSASQRALVGGVDQIYIDQVNPLWEIKQ"
     gene            1121..1387
                     /locus_tag="PK362_04890"
     CDS             1121..1387
                     /locus_tag="PK362_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74205.1"
                     /translation="MTCPCSEWEEIKEFNGPHEYDKFVGWIEQQVKNGKVKEIPVGHK
                     FGGFKERWFSCEVCRKVWQLVDHDFPFKGLFKVIEPTQSGRPDS"
     gene            1551..2453
                     /locus_tag="PK362_04895"
     CDS             1551..2453
                     /locus_tag="PK362_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74206.1"
                     /translation="MPLRQLLISYLFLYLFLTNAQALNKSPSAGVWLFDGAEGAKSFA
                     IVSVACDGEPGIFHEKPHTAKLPTHLGKTLAASAVGSSLVDRHPENLPRILSLGVASV
                     RSEFAQIPGWKDGGAKVANKDLLDQLFPSASTGAVVKLRDSKEEWLPGYLGLSPRLVS
                     GKERRLGPGFKLVPTKDIQRELAGLAEALNVDAVGFLRFDLAYSGTLSDSSGALVNGG
                     RANVGLEFVLLDRSGRVVLQTNDLEMTLRPRYMGGPVGPVVNGNVVLSDKTIDAYAQA
                     LASGARGLKNSILLAQKKNAKKTK"
     gene            2496..>2887
                     /locus_tag="PK362_04900"
     CDS             2496..>2887
                     /locus_tag="PK362_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNC74207.1"
                     /translation="MSRAFTKEDDAGEDLPEKPVSRGPNYVTPEGLKQLQDAARALGA
                     RRTKALAQGDDVKAFDRDLRYLEARIGGAIVVPPGAGVEVRFGASVSIEDESGRKQSF
                     KIVGEDEARAGGNLLAWSSPLAQALFGA"
BASE COUNT          676 a          829 c          796 g          586 t
ORIGIN      
        1 acccttcaac ggcgggcatt gggcttccaa acccctccct tcgccagcgt ctcctcaatt
       61 tcatctttgg ggagaaatta aaaaccgaat cggtttcccc caacgtcagc gcggaaggcc
      121 ctccgctctc gaaacccgac agcgcccatg ccgtaatcga cgacttggac aacagcctcg
      181 gctacattga cggcaagccg atgccgaccc ccgaccacct taaaaccttc aaccccggcg
      241 aaacgctccc ctccggcgac aaagccggga agggcccggg ggctgcatcc ctctccgaat
      301 acattgaaac caccctcact aaatccgaag tcatttccca gggacgcctg gggtttgacc
      361 ccgcgtttga aagcaagggc gcggcggaga gcggcatggt gggactctcc aaatccggat
      421 tggaggcctt ccgggttctt cgggaagccg gaatccgggt atccgtgggc tccgaagccg
      481 cctggggaga attgaacgcg gcaggctcgc acttcatcaa cgaagtcttt aacagcccga
      541 cccgaaccaa caaactcacc accacgtcgg atggcacgcg gttcctcgac atcaccgaca
      601 acaaggggcg gaccgtccga gccaccgaga agggcgactt cgtgggattc atcgtggacc
      661 gccccgaagc ccttccccaa tggaacccca aggcccttcc cccctcccgc gcggggcctc
      721 aatcaaaaac aaattttcag attttggttt cgagaaatcg agtaaaatac acacccatta
      781 atcctggacc acttccaaag gaaatcgctt caactttcag aagtgccacc tacgaagccg
      841 tgaagctcga tcaaccggcg gaactttaca gggtttttag cgatccgtcc aaaaaaatgg
      901 ggccctattg gaccattaaa aagccggaaa gttctttaaa agctatagtg gataatgctt
      961 tagatccaaa ttggggaaac agagcaaccg atatcgtttc aatacgagtt cccgcgggag
     1021 agacaattta tgttggaccg tcggcttcgc aacgtgccct tgtgggcggt gtagatcaaa
     1081 tttatattga tcaagtgaat ccactttggg aaataaaaca atgacttgcc cctgctccga
     1141 atgggaagaa attaaagaat tcaatggccc ccacgaatac gataagtttg tcggttggat
     1201 cgagcaacaa gtgaaaaatg ggaaagtcaa agaaattccc gtcggccata agttcggtgg
     1261 attcaaggag cgatggtttt cttgcgaagt ctgcaggaaa gtctggcaat tggttgacca
     1321 tgatttcccg ttcaaaggcc tctttaaagt tattgaacca acacaaagcg gaaggccgga
     1381 ttcttagcaa cttacaaatt gacaagcaac gtaggcttga gaatcaattg cttcgaccac
     1441 cgactcacga ctctttgaaa ttccagacaa agccggaaac ccgagccagc aatttccaat
     1501 cagcgtttga ccgggcagtt attggacagt cctacgacaa caaaaaaccg atgccccttc
     1561 gacaactatt aatttcctat ctctttctgt accttttttt aaccaacgcc caagcattaa
     1621 acaaatcccc ctctgcaggg gtttggcttt ttgatggggc cgaaggggcg aaatccttcg
     1681 ccatcgtctc cgtcgcctgc gacggggagc ccgggatctt ccatgaaaag ccccacaccg
     1741 ccaaactgcc gacccatttg gggaagaccc tagccgcgtc ggcggtgggg tcttcgctgg
     1801 tggatcgcca tccggagaac ttgccccgaa tattgagcct gggggtggcg tccgtccgta
     1861 gcgagttcgc tcagattccg ggctggaagg acgggggagc caaagtcgcc aacaaggacc
     1921 tgctcgatca attgtttcct tccgcctcca ccggagcggt ggtgaagctt cgtgattcca
     1981 aagaagaatg gcttccgggc tatctcgggc tttccccccg cctggtgtcg ggcaaagagc
     2041 ggcgcctcgg cccgggcttc aaattggtgc cgaccaagga cattcagcgc gagttggcgg
     2101 gcctggcgga ggctctgaac gttgatgcgg tggggtttct tcgattcgat ttggcctact
     2161 ccggcacctt gtcggacagt tcgggggccc tcgtgaacgg cgggcgggcc aacgtggggt
     2221 tggagttcgt gctgttggac cggtccggcc gggtggttct tcaaaccaat gacctggaaa
     2281 tgacgctccg cccacggtat atgggcggcc cggtgggtcc ggtggtcaac ggcaacgtgg
     2341 ttttgagcga taagactatt gacgcctacg cgcaggccct ggcctcgggg gcgcggggac
     2401 ttaaaaattc cattctgctc gctcaaaaga agaacgccaa gaaaaccaag tagctccccg
     2461 atcctttccc ctcccgggtt tcctatcctt tatccatgag ccgcgcattc actaaggaag
     2521 acgacgccgg ggaggacttg ccggagaaac ccgtttcccg tgggcccaac tacgtcacac
     2581 cggaaggcct aaaacaactg caagacgccg cccgggccct gggggcgcgg cgaaccaagg
     2641 ccctggccca gggcgacgac gtcaaagcct tcgatcggga tctgcgttat ttggaagcgc
     2701 gcatcggcgg ggccatcgtg gttcccccgg gggcgggcgt cgaagttcgg tttggggcga
     2761 gcgtttcgat tgaagacgaa tcgggaagaa agcagagctt caagatcgtg ggcgaggacg
     2821 aagcccgggc gggcgggaat ttattggcct ggtcttcccc cctggcccag gccctcttcg
     2881 gggccaa
//
LOCUS       DAOQLS010000209         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_19998_length_2872_cov_5.260561, whole genome shotgun sequence.
ACCESSION   DAOQLS010000209 DAOQLS010000000
VERSION     DAOQLS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19998_length_2872_cov_5.260561"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK362_04905"
     CDS             complement(<1..251)
                     /locus_tag="PK362_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74208.1"
                     /translation="MEPRPDDYFKGINFDKSWEEEDWEKFFDAQERLIRDYHWVGTPP
                     ERPAADPSLSFRQVLRRFGMDPDSPNTPPRSFHYTGEEPS"
     gene            complement(302..766)
                     /locus_tag="PK362_04910"
     CDS             complement(302..766)
                     /locus_tag="PK362_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNC74209.1"
                     /translation="MKTFTDYLWFTTPKRKDLVNITEEIEARVKRSGVREGFCLVSAM
                     HITAAIWVNDEESGLKEDVMAWLEKLAPASFPGDPARGKALSPLAPAEDYKHHRTGED
                     NADAHLKRTLVGHQAFLPITEGKLDLGPWEQVFYMEFDGQRRKRVVVKILGL"
     gene            complement(763..1155)
                     /locus_tag="PK362_04915"
     CDS             complement(763..1155)
                     /locus_tag="PK362_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNC74210.1"
                     /translation="MTARRWWAAVAVWCGIIFALSALPDRTQGGAPLPATMSRKMAHL
                     IEYGFLAFLTAQALRAGNRPRRIVLGGALLFCVLYAASDEFHQSFVPGRYAKVRDVAL
                     DALGAALVLSIYDRRALAPSTGKETPPP"
     gene            complement(1152..1835)
                     /gene="radC"
                     /locus_tag="PK362_04920"
     CDS             complement(1152..1835)
                     /gene="radC"
                     /locus_tag="PK362_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNC74211.1"
                     /translation="MTSSPNSAGHRARLRDRWRRAGSAGFPDYELLELLLTQAVPRRD
                     TKPLAKALLTRFGSLKGVLDASGPELESVPQVGPAVSFFFSVLRGTMERYFESEARKT
                     DLLNSPEAVIRYCRASLEGLKNEVFDVIFVSTKNRVVGRERLSEGTIDQAAVYPRRVV
                     ASALAANAASLIFVHNHPSGDPTPSAEDKQLTRRLEEAAQTVGIPVLDHLIVGNGGHF
                     SFREKGLLA"
     gene            complement(1819..2715)
                     /locus_tag="PK362_04925"
     CDS             complement(1819..2715)
                     /locus_tag="PK362_04925"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNC74212.1"
                     /translation="MRLGLHVSVRNGPVTALRRAAGLGAQTLQMFARHPRRWRGPGWD
                     HDTVAAFRQLRESLGLSPLLIHSAFVPNLSVSEEKSHRRSILHLAEELRRSEALGADA
                     FVIHAGAASPGRKPAEGVERFRDAVREASALAPGRVSILLENVPGGGRRLGGDFAEVA
                     ALLNALPSNRRPGLCFDTGHAWGAGWDLSTPSGIGETVKALDRSVGLSAIRVFHVNDS
                     AAPRGSGLDWHNHLGKGLIGIENLRAFFARADRRDAAYIMETPRDAIDADEKNFETMA
                     RGLAGLFDGGPSDKMSPNDLLP"
     gene            2779..2866
                     /locus_tag="PK362_04930"
     tRNA            2779..2866
                     /locus_tag="PK362_04930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2812..2814,aa:Leu,seq:caa)
BASE COUNT          574 a          891 c          920 g          487 t
ORIGIN      
        1 gagggctctt cgccggtata gtgaaaggac cggggcggcg tgttgggact gtcgggatcc
       61 atgccgaagc ggcggagaac ttggcggaag gacagggagg ggtccgccgc ggggcgttcc
      121 gggggggtgc ccacccagtg ataatcccgg atcaggcgtt cctgggcgtc aaaaaacttc
      181 tcccaatcct cttcctccca ggatttgtcg aagttgatcc ctttaaaata atcgtccggc
      241 cggggttcca tgcccccatt ataccacagc ctcgtcggaa aacgaccccg cgggtcccgg
      301 tttagaggcc gaggatcttc acgaccaccc gtttgcggcg ttggccgtcg aattccatgt
      361 aaaaaacctg ttcccagggc cccaaatcca gtttcccctc cgtgatgggc aaaaaggcct
      421 ggtgccccac cagcgtgcgt ttcaagtggg cgtcggcgtt gtcttccccg gtgcggtggt
      481 gtttgtagtc ctcggccggg gccagggggc tgagggcctt accccgggcc ggatcgcccg
      541 gaaaggaagc cggggccagc ttttccagcc aggccatgac gtcttcttta agaccggatt
      601 cctcgtcatt gacccaaatg gccgccgtga tgtgcatggc ggaaacaagg cagaaaccct
      661 cccgaacgcc gcttctcttc acccgggctt cgatttcctc cgtgatgttg acgaggtctt
      721 tgcgtttggg cgtcgtgaac caaaggtaat cggtaaaggt tttcatgggg gcggtgtttc
      781 ctttccggtc gatggggcca atgcccggcg gtcgtaaatc gagaggacga gagccgcgcc
      841 cagggcgtcc agggccacgt cccgaacctt ggcataccgg cccggcacaa aggattgatg
      901 gaattcatcc gacgccgcgt agagaacgca aaaaaggagg gcgcccccca gcacgatccg
      961 ccggggacgg ttccccgccc gcagggcctg ggcggtcaaa aaagccaaaa aaccatactc
     1021 gatcaaatgg gccatttttc gggacatcgt cgccgggagg ggcgccccgc cctgggttcg
     1081 atccggaagg gccgaaaggg cgaagatgat cccgcaccac acggccaccg cggcccacca
     1141 tcggcgggcc gtcacgcgag caggcccttt tcccggaagc tgaaatggcc gccgttgccg
     1201 acgatcaaat ggtccaacac cgggatgccc accgtttggg cggcttcctc cagtcggcgg
     1261 gtgagttgct tgtcctccgc ggacggcgtg ggatccccgg aggggtgatt gtgaacaaag
     1321 atgagggaag cggcgttggc cgccagggcc gaagccacca cccggcgcgg gtaaacggcc
     1381 gcctggtcga tggttccctc cgacagacgt tcccggccca cgacccggtt tttggtcgaa
     1441 acaaaaatca cgtcaaagac ttcgttcttg agcccctcca gggaagcccg gcagtagcgg
     1501 atcacggcct cgggtgaatt gagcaagtcg gttttccggg cctcggattc gaaatagcgc
     1561 tccatggtgc cccgaaggac cgaaaagaag aaagacaccg ccggaccaac ctggggaacg
     1621 gattccagct ccggtcccga agcatccaag acgcccttca aagatccaaa acgggtcaaa
     1681 agcgccttgg ccaggggttt ggtgtcccgg cggggcaccg cctgggtcag caataattcc
     1741 aatagttcgt aatcgggaaa gccggccgaa ccggcccgac gccaacggtc ccgcagacgg
     1801 gcccgatgac cggcggaatt aggggaggag gtcattcggc gacattttat cggaaggtcc
     1861 gccgtcaaac aagcccgcca ggccgcgggc catcgtttca aaattttttt cgtccgcgtc
     1921 gatcgcgtcc cggggcgttt ccatgatgta agcggcgtct cgtcgatcgg cccgggcaaa
     1981 aaacgcccgg agattttcga tgccgatgag acctttcccc aggtgattgt gccaatccag
     2041 tccgctcccc cggggagccg cggaatcgtt gacgtggaaa acccggatcg ccgaaagacc
     2101 caccgatcgg tccaaggcct tcacggtctc cccaatgccc gacggcgtgg acaggtccca
     2161 ccccgcgccc caggcgtggc cggtgtcgaa acaaagaccg ggccggcgat tggagggaag
     2221 cgcgttcaga agggcggcga cttcggcaaa atcccccccc aggcggcggc ctccgccggg
     2281 aacgttctcg agcaatatcg acacccggcc gggggccaac gcggaggcct cccggacggc
     2341 gtcccgaaac cgttcgacgc cctcggccgg tttccgcccg ggcgaggccg cgccggcgtg
     2401 aatcacaaag gcgtcggcgc ccagggcctc ggaccgacga agctcctccg ccaaatggag
     2461 gatggaccgc cgatgggact tctcctccga aacggagaga ttgggaacga aggcggagtg
     2521 aatgaggagg ggcgaaagtc ccagggattc ccgaagttgg cggaaggcgg cgaccgtgtc
     2581 gtggtcccac cccggccccc gccaccgccg ggggtgccgg gcgaacatct gaagggtttg
     2641 ggcgcccaac cccgccgccc gacggagagc cgtcacggga ccgtttctga ccgacacgtg
     2701 gagtccaagg cgcatggttt gttgacaatc ctcgacggaa aagagaaaat gttattgttt
     2761 cggtttggca gcgcccttgc cggggtggcg aaatggcaga cgcgtgagac tcaaaatctc
     2821 aagtggctta ggccacgtgg gggttcaagt cccccctccg gcaccaattt aa
//
LOCUS       DAOQLS010000210         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20114_length_2862_cov_4.542216, whole genome shotgun sequence.
ACCESSION   DAOQLS010000210 DAOQLS010000000
VERSION     DAOQLS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20114_length_2862_cov_4.542216"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1888
                     /locus_tag="PK362_04935"
     CDS             116..1888
                     /locus_tag="PK362_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNC74213.1"
                     /translation="MPSVNELKEKLINTQIWRSMFRGGTWKDTSRDRAQHILGNVWLH
                     LHPARIRQRAVRFTYTWGLGGLSFGLFMILTITGILLMFYYRPAVELAYRDMKDLEFA
                     VSMGLFLRNLHRWSAHTMVLLVILHMVRVFLTGAYKTPREFNWGVGVVLLVLTLFLSF
                     TGYLLPWDQLAIWAVTVGTNMASATPFLGAEGPFSSLLGVNGSNDARFALLGGTSVGA
                     NTLLRFYVMHCVAVPVVAGILMIVHFWRIRKDGFSAAPLAGDPPEKKMEVWPNLVVRE
                     YIGAILMLIFLVGWSVLQNAPLEELANPTVTPNPSKAPWYFLGLQELLVYFDPWLAGV
                     KLPGIIALGLIAIPYLDPDPKAGVGYYSIKERPFANTVFLFGIGLWFVLITIGTFMRG
                     PNWAWYWPWESWASHKPLPPPTWSLILVNLDGKMGQFFTSQKTAGLQIPGWAGVGAAL
                     AAAGGLFTTYFGAGIVLPKLVNKDLPFKKSVGIAVIASVATGVLAKTAHTFLAKYDFI
                     GKNMPHFGVTEIFYIAFFLSIVIVMGFLLPQRYLRKSPVSLVQYVVTMVLLLSMLGVV
                     LKMGARLGFNIKYLFSLPGLNLNI"
     gene            1885..>2862
                     /locus_tag="PK362_04940"
     CDS             1885..>2862
                     /locus_tag="PK362_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC74214.1"
                     /translation="MTLKETLLTRYFTLRNLFVGGNVLLLALFLAAVVKDSRREWKDF
                     QKAYYAKEIKRLSEALDGANTPEEKDRLTKKLALVRAQPVRIRQLIVPGMDRYDRCTT
                     CHLGFDPVLSPTGETSFTEQPFSARANDVHKSHPVEKFGCTVCHEGQGLATTVQAAHG
                     PVPHWEKPLHRGVYLQAACAKCHSNLHDEKAMPFTAAWRRGETLFKEKGCIGCHQIHG
                     QGGPISVDLAEETADKPLSRIDWSHTGLGEEERTLAEWIRLHLATDPWTLVPGDPKAE
                     FNEEPIAPSGMPFFQLSDEDARAVTTYVLSYAKDRIPSQVVVPSAPRPEP"
BASE COUNT          511 a          922 c          805 g          624 t
ORIGIN      
        1 gtgctccgtc gatgtcacgc ccgacgggga tttggtcatc gataagtcgc aaatggaaaa
       61 tcgacccgga ttccgggacc gcggcgcctt cttcatcccg gtggccgttt aagccatgcc
      121 ctccgtcaac gaactcaaag aaaaactgat caacacccaa atttggcgct ccatgttccg
      181 gggcggcacc tggaaggaca cctcccgcga tcgcgcccag cacatcctgg gcaacgtctg
      241 gctccacctc catccggccc gcattcgaca gcgcgccgtg cgctttacct acacctgggg
      301 gctgggcggc ttgtccttcg gcctgttcat gattttgacc atcacgggca ttttgctgat
      361 gttctactac cgcccggcgg tggagctggc ctaccgcgac atgaaggatc tggaattcgc
      421 cgtttccatg gggctctttc tgcgaaacct ccaccggtgg agcgcccaca ccatggtgct
      481 cctggtcatt ctccacatgg ttcgggtgtt cttgacgggc gcctacaaaa cgccccggga
      541 gttcaactgg ggcgtggggg tcgtgctcct ggttctcacc ctgttcctct ccttcacggg
      601 ctaccttctc ccctgggatc aattggccat ctgggccgtg accgtcggaa ccaacatggc
      661 cagcgcgacg ccgtttctgg gggccgaggg gcctttctct tccctgctgg gcgtcaacgg
      721 ctccaacgac gcccggttcg cccttttggg cggcacctcc gtgggcgcga acacccttct
      781 gcgcttttac gtcatgcact gcgtggccgt cccggtggtg gccgggatcc tcatgatcgt
      841 ccacttctgg cgaatccgga aggacgggtt ttcggccgcg cccttggccg gcgatccgcc
      901 cgaaaaaaag atggaggttt ggcccaacct cgtggttcgg gaatacatcg gcgccatttt
      961 aatgttgatt ttcctggtcg gctggtccgt gcttcaaaac gctccgttgg aagaattggc
     1021 caaccccacg gtgaccccca acccgtccaa ggccccttgg tatttcctgg gccttcaaga
     1081 gctcctggtc tatttcgatc cctggttggc cggcgttaaa ttgccgggga ttatcgccct
     1141 ggggttgatc gccatcccct acctggatcc ggatcccaag gccggggtcg gttattactc
     1201 catcaaggaa cgcccttttg ccaacaccgt tttccttttc gggatcggtt tgtggttcgt
     1261 gttgatcacc atcggcactt ttatgcgggg ccccaactgg gcctggtact ggccctggga
     1321 aagctgggcc tcccacaagc ccctgccgcc gcccacctgg agcctcattc tcgtcaatct
     1381 cgacggtaaa atgggccaat tcttcacgag tcagaaaacc gcgggcctgc agatccccgg
     1441 ttgggcgggc gttggcgcgg ccttggccgc cgcgggcggt ctgttcacga cctatttcgg
     1501 cgccggcatc gtcctgccca agctggtgaa caaagacctg cccttcaaaa aatcggtggg
     1561 catcgcggtc attgcctcgg tggcgacggg ggtcctggcc aaaaccgccc acaccttcct
     1621 cgcgaaatac gattttatcg gcaagaacat gccccacttc ggcgtgacgg aaatcttcta
     1681 catcgccttt ttcctgtcca tcgtgatcgt catggggttt ctgcttccgc aacgctatct
     1741 tcgtaagtcc ccggtcagcc tcgttcaata cgtggtgacc atggtccttt tgttgtccat
     1801 gttgggcgtg gtccttaaaa tgggggcccg gctgggtttc aacatcaaat acctgttcag
     1861 cctcccgggg ctcaatctca acatatgacg ctgaaagaaa ccttgctcac ccgctatttc
     1921 acccttcgca atctattcgt cgggggaaac gtcttgctct tggccctctt cctggcggcg
     1981 gtggtgaagg attctcgccg ggaatggaag gatttccaga aggcctatta cgccaaggaa
     2041 atcaaacgct tgtccgaggc cctggacggc gccaacactc ccgaagagaa ggaccggttg
     2101 accaagaaac tggcgttggt tcgggcccag cccgtccgaa ttcggcagtt gatcgttccg
     2161 ggaatggacc ggtacgaccg ttgcaccacc tgccatctgg gcttcgaccc ggtgttgagc
     2221 cccacggggg aaacgtcttt cacggaacag cccttttcgg cgcgcgccaa cgacgttcac
     2281 aagtcccacc ccgttgaaaa attcggctgc acggtttgcc acgaaggcca gggcttggcc
     2341 acgacggtcc aggccgccca cggtccggtc ccccattggg aaaaacctct ccaccgaggc
     2401 gtgtatttgc aggccgcctg cgccaaatgc cactcgaatt tgcacgacga aaaggccatg
     2461 ccgttcaccg cggcctggcg ccggggcgaa actttgttta aggaaaaggg atgcatcggt
     2521 tgccaccaaa ttcacggtca gggcggtccg atttccgtcg atctcgcgga agaaaccgcc
     2581 gacaagccgc tttcccgcat tgattggagc cacacgggcc tcggggaaga ggagcgcacc
     2641 ctggccgaat ggatccggct ccacctggcg acggacccct ggacgctggt tcccggcgat
     2701 cccaaagcgg aattcaacga agagcccatc gcgccttcgg gcatgccgtt cttccagctt
     2761 tcggacgaag acgcccgcgc ggtcacaacc tacgtcttgt cctacgcgaa ggaccgtatc
     2821 ccgtcccaag tggtggtccc gtcggccccc cgtccggagc cg
//
LOCUS       DAOQLS010000211         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20199_length_2856_cov_3.921100, whole genome shotgun sequence.
ACCESSION   DAOQLS010000211 DAOQLS010000000
VERSION     DAOQLS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20199_length_2856_cov_3.921100"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK362_04945"
     CDS             complement(<1..314)
                     /locus_tag="PK362_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74215.1"
                     /translation="MSRFQSSETRSSLWAGAVALALLVAANGAPTGRGGFPPFDHPVL
                     DEFVLQDTAFVLSGARRLGADLAFIQLLLYYGGADLREKHDRHAGEDPAEAGFGTLGE
                     LDL"
     gene            complement(314..1078)
                     /locus_tag="PK362_04950"
     CDS             complement(314..1078)
                     /locus_tag="PK362_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC74216.1"
                     /translation="MTSIFSIARYTLTQQLRNRLYLVIILFGVLMVGASVLFGAVAAD
                     QEIRVILDLGFATTEFFGLAMALFGAVTLVLEEMESKTIYLILTRPQPRATYILGRFL
                     GLVLAVWTGMALMEVMHFALLLFKGWKADPLTFAALPAMGLKVMIMTALALLCSLLFT
                     SAPTATVFSLFFWVLGHFGEEIRFLAKKSGQPVAMALVKGLLWILPNLTMLNLRDHLE
                     IPGFPLTQILPAALYALLYTGAALSLSIALFARKEF"
     gene            complement(1078..1842)
                     /locus_tag="PK362_04955"
     CDS             complement(1078..1842)
                     /locus_tag="PK362_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC74217.1"
                     /translation="MTADTPALAVTDLTKIYRRSHLGRTKTTRGVEKINLAVRDGEVF
                     GLLGLNGSGKTTTIKLLLGLLFPTSGRIDVLGAPAGSRAAKAAIGYLPEIPYFYKYLS
                     GREVLTFYGQLSGLTGEALRQRVDAALDVVRMKDHARRAMREYSKGMLQRIGLAQSFL
                     HEPKILIYDEPVTGLDPLGLREMREIIQGLNQKGKTIFFSSHSISEVEKLCHRVGILV
                     QGRLVRVVEQKEWAAEPGRLEEVFVQTVSPDHAHVR"
     gene            complement(1864..>2856)
                     /locus_tag="PK362_04960"
     CDS             complement(1864..>2856)
                     /locus_tag="PK362_04960"
                     /inference="COORDINATES: protein motif:HMM:NF039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC74218.1"
                     /translation="FITMHVPLSDQTRGLLNRDTFAKTKKGVRVINCARGGIIDEKAL
                     LEFVENGHVKGAALDVFEKEPLDPQSPLLKRPEIIVTPHLGASTEEAQVKVAVELSQS
                     LVEFFENGFAKQAVNLPPLDVAGQNQLLSFIGLAHKQGAFLAQFAGAAATSLTLRYSG
                     ELARVNPSLYTATAVAGFLAALGEKATPVNALLLAQQRGLRVQEQVQPEAKDYASLLE
                     MEAETPEGSHQLAGTVYGRGDTRFVRIDGLAVDVTPHKYLLVMTNEDRPGVVGHTGTV
                     LSAAGINIAGMDIARNRPGGVAVSLWSVDSPVPAEVLAKVKAFAPILSVKMVQL"
BASE COUNT          570 a          823 c          950 g          513 t
ORIGIN      
        1 agatcaagct ctcccagggt gccaaacccg gcctcggcgg ggtcctcccc ggcgtggcga
       61 tcgtgtttct cccgaaggtc ggccccgccg tagtaaagga gcagttgaat gaaagccagg
      121 tcggcgccga gtcgacgggc gccgctcaag acgaaggcgg tgtcttgcag gacgaattcg
      181 tccaagaccg gatggtcaaa gggcgggaat cccccccgtc cggtcggcgc cccattggcc
      241 gcgacgagca acgccagggc gacggccccg gcccaaaggc ttgagcgggt ttccgacgac
      301 tggaagcggc tcattagaat tctttgcggg cgaagagagc gatggacagc gacaacgcgg
      361 cgccggtgta aagcagtgcg taaagggcgg cgggaaggat ttgcgttaac gggaacccgg
      421 gaatttccag atggtcgcga agattgagca tcgtgagatt gggcagaatc cacaacaggc
      481 ccttcaccag ggccatcgcc accggttgac ccgatttttt ggccaggaag cggatctcct
      541 ccccgaaatg gcccaggacc cagaagaaga gcgaaaacac cgtggcggtc ggggcggacg
      601 tgaacagaag cgagcacagg agggccaacg cggtcatgat catgactttt aggcccatgg
      661 ccggcaaagc ggcgaaggtc aaggggtccg ctttccaacc cttgaataac aggagcgcga
      721 aatgcatgac ttccatcagg gccatgcccg tccacacggc gagaacgagg ccgagaaaac
      781 ggccgagaat gtaggtggcg cgcggttggg ggcgcgtgag gatcaagtaa atggttttcg
      841 attccatttc ttccaggacg agggtcaccg cgccgaaaag cgccatggcc aacccgaaaa
      901 attccgtcgt ggcgaatccc agatccaata tgacccggat ttcctgatcc gccgcgacgg
      961 cgccgaaaag cacggacgcc ccgaccatga ggacgccgaa caggatgatc accaggtaca
     1021 ggcggtttcg caattgctgg gtcagcgtgt aacgcgcgat ggagaagatg gaagtcatta
     1081 gcggacgtga gcgtggtcgg gggagaccgt ttgaacgaac acctcttcca aacggcccgg
     1141 ttccgcggcc cattccttct gctccaccac gcgaacgagc cgaccttgga ccaggatgcc
     1201 gacccggtgg cacaattttt caacttcgga aatgctgtgg gacgaaaaga agatcgtttt
     1261 gcctttttga ttcaacccct gaatgatttc ccgcatttcg cggagcccca ggggatccaa
     1321 gccggtcacg ggctcgtcgt aaatcaaaat tttgggttcg tgcagaaaag attgcgccaa
     1381 gccgatgcgc tgcaacatgc ctttggaata ttcccgcatg gcgcgccggg cgtggtcttt
     1441 catgcgcacg acgtccaagg cggcgtcgac ccgttggcgg agggcctcgc ccgtgaggcc
     1501 ggaaagctgg ccgtagaacg tgagaacttc ccgaccggaa aggtatttgt agaaataggg
     1561 gatttcgggc aggtagccga tggcggcctt ggccgcccgg gacccggcgg gggcgccgag
     1621 cacatcgatt cggccgctgg tgggaaacaa caggccgagc aggagcttga tggtggtggt
     1681 ttttccggac ccgttcaatc caaggaggcc gaagacttcg ccgtcccgga cggcgaggtt
     1741 gattttttcc acgccgcgcg tggttttcgt ccgtcccagg tgggaacggc ggtagatctt
     1801 cgtgaggtcg gtgaccgcga gggccggagt gtctgccgtc atggggcgtt caaaaatcga
     1861 acgctataac tgtaccattt tgaccgacaa gatgggcgcg aaggccttga ccttggcgag
     1921 aacctcggcg ggcaccgggc tgtccacgct ccaaagcgag accgccacgc cgcccgggcg
     1981 gttgcgggcg atgtccatgc ccgcgatgtt gatccccgcg gcggagagca ccgttcccgt
     2041 gtgccccacg accccgggtc ggtcctcgtt ggtcatgacc aaaaggtatt tgtgcggcgt
     2101 cacgtccacc gcgaggccgt cgatgcggac aaagcgcgtg tcgccgcggc cgtacaccgt
     2161 gcccgccagt tggtgggacc cctcgggggt ttcggcctcc atctcgagga gggaggcgta
     2221 gtccttggct tcgggttgaa cctgctcctg gacccgcaga ccgcgttgtt gggcgagcaa
     2281 gagggcgttg accggcgtgg ctttttcccc caaggccgcg aggaatccgg cgacggcggt
     2341 ggcggtgtaa agcgagggat tgacccgggc cagctccccg gaatagcgga gggtgagcga
     2401 ggtggccgcc gccccggcga attgggcgag gaaggccccc tgcttgtgag ccaggccgat
     2461 aaaggacagc aattggtttt ggcccgccac gtccaagggc ggaaggttga cggcctgttt
     2521 ggcgaatccg ttttcaaaaa attccaccag ggactgggac agttccaccg ccaccttgac
     2581 ctgggcctct tcggtcgagg cgcccaggtg gggcgtcacg atgatctcgg gccgcttcaa
     2641 caggggcgac tgcgggtcca acggttcctt ctcgaagacg tccagggccg cgcccttgac
     2701 gtggccgttt tcaacgaatt ccagcagcgc cttttcgtcg atgatgccgc cccgggcgca
     2761 gttgatgacc cgaacccctt ttttggtttt ggcgaaggtg tctcggttca gcaggccgcg
     2821 ggtttggtcc gacaagggca cgtgcatggt gatgaa
//
LOCUS       DAOQLS010000212         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20277_length_2850_cov_3.873345, whole genome shotgun sequence.
ACCESSION   DAOQLS010000212 DAOQLS010000000
VERSION     DAOQLS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20277_length_2850_cov_3.873345"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PK362_04965"
     CDS             <1..796
                     /locus_tag="PK362_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004932884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC74219.1"
                     /translation="AHPKPNRLRSVLGLGAKNAAIVLPDADLDLTVKEGVAGALTFNG
                     QRCTALKIFFVHRSVADRFVSKMAEAVAALRRGLPFEDGVQITPLPDVAMVEKMGRYV
                     ENARSKGARVANPGGGESERTFFSPAVVYPVAPSMALWREEQFGPVVPVAAYESLDEV
                     IDYLAGSDLGQQASVFGREPKALGGLIDALVNQVCRVNLNAQCRRGPDTFPFGGRKDS
                     AEGTLSVTDALRVFSLRTLAAGVDRPETRDLMERLAEGGFSRFLSR"
     gene            793..1434
                     /gene="pnuC"
                     /locus_tag="PK362_04970"
     CDS             793..1434
                     /gene="pnuC"
                     /locus_tag="PK362_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNC74220.1"
                     /translation="MTALFSTGTVLFRVAGYAMSPVEFLGTLLTLWSVWLVTRRNVWT
                     WPVGNLGGLLFAFLFYQIRLYSDLVEQIYYIATGFYGWWVWTRRGAAAEADLSVTVAS
                     PRLRAVSLGAIVIGTALMGFVMSRVNVWWPLWFPETASYPYWDAFTTVVSFTAQLLMA
                     HKKLDCWPLWVLVDVIGIALYFVKGVVFVSFLYVIFLGLALNGWRSWRRTRTA"
     gene            1431..2423
                     /locus_tag="PK362_04975"
     CDS             1431..2423
                     /locus_tag="PK362_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC74221.1"
                     /translation="MTEGLVIGKFYPPHRGHGYLIDTALQNADRVTVLVCEKKEETIS
                     GALRARWIREMAPAARVRVIPDIGADDDSEAWALHTRRFLGYRPDLVFTSERYGPVFA
                     RHLGARHRMVDLKRTTVPIAARHIRADPEKYWNFLSPGAKAHYAKRVVVLGAESTGKT
                     TLARALAARLKTNWVPEFGRYHYEGKAAAGTPLRWSDEDFIHIARTQNQWEEALARTC
                     QGWLVCDTNAFVTSVWQRRYLGRVSPEVEREARRGRYHAALLLEDDLPFVQDGTRDGK
                     SFRRRMQKMLVEELNRRKIPVVRIRGRGPRRLAAAWAALQKSEFLPPTMEREKV"
     gene            2431..>2850
                     /locus_tag="PK362_04980"
     CDS             2431..>2850
                     /locus_tag="PK362_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74222.1"
                     /translation="MPVDRTLVRFFFNPLNRRPGPVLYFLPAVFFFVLSARAEEPGPM
                     ALGLNYPGASLRFHLNERYALEGRFQAESGVWAGGVRGYRYFSTLGRVHPFAGLEGDY
                     LGFKENAAKGSGFAGIAFAGGEYFFARRLSAQLDMGAG"
BASE COUNT          435 a          929 c          909 g          577 t
ORIGIN      
        1 cgcccacccg aaacccaacc ggctccgttc cgtcctgggc ctgggagcaa aaaacgccgc
       61 catcgtgctg cccgacgccg atctcgactt gacggtcaag gaaggcgtgg cgggggccct
      121 caccttcaac ggccaacgtt gcacggccct taaaatcttt ttcgttcacc gatccgtcgc
      181 cgaccgcttc gtgtcaaaaa tggccgaggc cgtggcggcc cttcgccggg gcctgccttt
      241 cgaggacggc gtccaaatca cgccgcttcc cgatgtggcc atggtggaaa aaatgggccg
      301 ttacgtggag aacgcccggt ccaagggcgc ccgggtggcg aacccgggcg ggggcgaaag
      361 cgagcggacg ttcttttccc cggcggtggt ttatcccgtg gcgccttcca tggccctttg
      421 gcgggaggag cagttcggcc ccgtggtgcc cgtggcggcc tacgaatccc tggacgaagt
      481 gatcgactat ttggcggggt ccgacctggg ccagcaggcc agcgtcttcg gccgggaacc
      541 caaagccctg ggcgggttga tcgacgccct ggtcaaccag gtctgccggg tgaacctcaa
      601 cgcccaatgc cgccgggggc cggacacctt tcccttcggc gggcggaaag attccgccga
      661 gggcaccctg tcggtgacgg acgccctgcg ggtcttctcc cttcgcaccc tggccgcggg
      721 cgtggaccgg cccgagaccc gggacttgat ggaacggctg gccgaaggcg gtttctcgcg
      781 ctttctgtcc cggtgacggc gcttttcagc acgggcaccg tgctgttccg ggtggcgggc
      841 tacgccatga gcccggtgga atttctgggg accctgctga ccctctggtc cgtctggctg
      901 gtgacacgcc ggaacgtctg gacctggccc gtcggaaatc tgggcggcct ccttttcgct
      961 tttctttttt atcagatccg gctgtactcc gacctggtgg agcagatcta ttacatcgcc
     1021 accggttttt acggctggtg ggtctggacc cgccggggcg ccgccgccga ggcggacttg
     1081 tccgtcaccg tggcctcgcc ccgcctccgc gccgtttcgc tgggcgccat cgtgatcggc
     1141 accgccctca tgggattcgt catgtcccgg gtcaatgtct ggtggcccct ttggtttccc
     1201 gaaaccgcct cctaccccta ttgggacgcc ttcaccaccg tggtcagttt caccgcccaa
     1261 ctattaatgg cccacaagaa attggactgc tggcccctct gggtgctggt ggacgtcatc
     1321 ggcatcgccc tgtattttgt gaagggcgtg gttttcgttt cttttctcta cgtcattttc
     1381 ctggggctgg ccctgaacgg ttggcgttcc tggcgccgga cgaggacggc gtgacggaag
     1441 ggttggtcat cggcaagttc tacccgcccc accggggaca cggttatttg atcgacaccg
     1501 cgctccagaa cgcggaccgg gtgacggtgt tggtctgcga aaaaaaagag gaaactattt
     1561 ccggcgccct tcgggcccgg tggattcggg aaatggcgcc cgccgcccgg gtgcgggtga
     1621 tccccgacat tggcgccgac gacgattccg aggcctgggc cctccacacc cggcgttttt
     1681 tgggctatcg gcccgatctg gtgttcacct ccgaacggta cgggcccgtc tttgcccgcc
     1741 atctcggggc acgacaccgg atggtcgatt tgaaaaggac caccgttccc atcgccgccc
     1801 ggcatatccg cgccgatccc gaaaaatact ggaacttcct ttcccccggg gccaaagccc
     1861 actacgccaa acgcgtcgtg gtgttgggcg ccgagtccac gggcaagacc accctggccc
     1921 gggccctggc ggcccgtttg aaaaccaact gggtgccgga gttcggccgc tatcactacg
     1981 aggggaaggc cgccgccgga acgcccctcc gttggtccga cgaggatttt atccacattg
     2041 cccggaccca aaaccaatgg gaggaggccc tggcccgcac ctgccagggg tggctcgtgt
     2101 gcgacaccaa cgcctttgtg acgtcggtgt ggcagcggcg ttatttggga cgcgtgtcgc
     2161 cggaagtgga acgggaggcc cgccggggcc gttaccacgc ggctctgctc ctggaggacg
     2221 acctgccctt cgttcaggac gggacccggg acgggaaaag ctttcgacgt cggatgcaga
     2281 aaatgttggt ggaggaattg aatcgtcgaa aaatccccgt ggttcgaatt cgcggccggg
     2341 ggccccggcg gctggccgcg gcctgggcgg ccctgcaaaa gtcggaattc ctccctccga
     2401 cgatggaacg ggaaaaagta taatttcccc gtgcccgttg accgaaccct cgtccgcttt
     2461 tttttcaacc ccttgaaccg ccgccccgga ccggttcttt acttcctccc ggccgttttt
     2521 ttctttgtcc tttccgcccg ggccgaggag cccgggccga tggccctcgg tctcaactat
     2581 cccggcgcgt ccctccgttt tcatctaaac gagcgctacg ccctggaagg acggtttcag
     2641 gcggagtccg gcgtttgggc cggaggcgtt cgtggctacc ggtacttttc gaccctgggt
     2701 cgcgttcatc ccttcgcggg cctcgagggc gattacctgg gttttaagga aaacgccgcc
     2761 aaggggtccg gcttcgccgg gatcgccttc gccggggggg aatatttctt cgctcgacgc
     2821 ctgtcggctc agctggacat gggcgcgggg
//
LOCUS       DAOQLS010000213         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20395_length_2840_cov_4.338959, whole genome shotgun sequence.
ACCESSION   DAOQLS010000213 DAOQLS010000000
VERSION     DAOQLS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20395_length_2840_cov_4.338959"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1950)
                     /locus_tag="PK362_04985"
     CDS             complement(70..1950)
                     /locus_tag="PK362_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC74223.1"
                     /translation="MKRLILVVTLAAAGFLRAEDGGVFLSLTRSATPDKRLPTNVSRM
                     TADDWESLGATALDEGVVPFPSVVVQKSGGAGSFATLRLRGSPSSAQQQILIDDLPLL
                     GISNQFFDLSQMPLTDIDRIEVVRGGASVLYGANTTGGVVHLLTKRPVGDKPQTRLKS
                     EFRSYYTQLYEGDIRHRVGGFDAYASAGRSLTDGFQKNADSDNLHGTLKTGYTFGSGA
                     RLAIDGAIVDGEVGNPHGTLLPVEEWDGKKEREAVDPTARVQQKAQRGHGLVFLPLGG
                     SASVQSTFYGTGQRYETRPSKTAAPDFEQDNRFWGNDTRFQWSDVITLGAAYEWDGQK
                     TAESAFAPARNNHITNVGAYGQLSLALGAWVFMPGLRYDSHSAFGPTWNPRLTVAWRA
                     TENLKLSGNAARSFRAPSFLELYYVDAFFSGNPDLRPEKAWSYDFGFEVGGNAARRLS
                     VTGFYTKIEDRVTVNGAFTSYENAPQAELAGAEVESRHPLLPRVTGRASYTYTRAIGN
                     SLTESTHRALRLTPRHAAFYQAAWRPGNEWELSSAVRYVSRQFQKDDQRGLDLPTFAV
                     WGARLSKKILAAEFYVACDNILNRRYALTFDAESTFPFSTTRNPQPGRTFWMGTTIRF
                     AD"
     gene            complement(1965..2765)
                     /locus_tag="PK362_04990"
     CDS             complement(1965..2765)
                     /locus_tag="PK362_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC74224.1"
                     /translation="MTVLSVKNLSFAYPRWEDDRPFALKGISFDMEAGDLLAVLGPNG
                     AGKSTLLRLLGGTLGPGSGEIRLGNDSLESLSAREVARRVAWVPQDLSTLFSMTVEDV
                     VSLGRFCHGPLWGRRSIADRREVHRALEETDLTGLRHRPVDRLSGGERRRVLLARALA
                     QEPHLLLLDEPTAHLDPRHQAELVAVIERLRRERRLAVIAILHDVNLAYGWCPRALLM
                     DKGECRALGPTARVLSSEQLSATYGLEASVRPGDSGTAGFIQFHYSKK"
BASE COUNT          518 a          902 c          947 g          473 t
ORIGIN      
        1 ccccgccggt ccccaaaaaa aggcccctcc ccccgctgtc ggcggaagga ggggcctcga
       61 gcttttcctc tagtccgcga aacggatcgt cgtgcccatc caaaaagtcc ggccgggctg
      121 ggggttccgg gtggtcgaaa agggaaaagt cgattccgcg tcgaaggtca gggcgtaacg
      181 gcgattcaga atattgtcgc aggccacgta aaactccgcc gcgagaattt tcttggacaa
      241 tcgggcgccc cacaccgcga acgtcggcag atcgagcccc cgctggtcgt ctttttgaaa
      301 ctgcctcgac acgtaacgaa ccgcgctgga gagctcccac tcgttgccgg ggcgccaagc
      361 cgcctggtag aaggccgcgt ggcggggcgt gagccgcagg gcccggtggg tcgactcggt
      421 caaggaattg ccgatggccc gggtgtaggt gtaggaagcc cgtcccgtca cccgcggaag
      481 gagcgggtgg cggctttcaa cctcggcccc ggcgagttcc gcctggggcg cgttttcgta
      541 ggaggtgaag gcgccgttga ccgtgacccg gtcctcgatt ttcgtgtaga agccggtcac
      601 cgacaggcgt cgggcggcgt ttccgccgac ctcgaaccca aaatcgtagg accaggcttt
      661 ttccggacga agatcggggt tgccggagaa gaaagcgtcg acgtaataga gctccaggaa
      721 ggagggggcc cggaaggaac gggcggcgtt gccggagagt tttaaatttt ccgtcgcgcg
      781 ccaggccacc gtcaaccgcg ggttccaggt gggaccgaaa gcgctgtggc tgtcgtaccg
      841 caggcccggc atgaacaccc aggctcccag ggcgaggctc agctggccgt aggcgccgac
      901 gttggtgatg tgattgttcc gggcgggagc gaaagccgat tcggccgttt tttgaccatc
      961 ccattcgtag gccgccccca gggtgatcac gtcggaccat tggaagcggg tgtcgttccc
     1021 ccagaaccgg ttgtcctgct cgaaatcggg cgcggcggtt ttggacgggc gcgtttcgta
     1081 ccgttggccc gttccataaa aagtcgactg aacgctggcc gatcccccca gcggaaggaa
     1141 gacgagcccg tgtccgcgct gggccttttg ctggacccgg gccgtcggat ccacggcctc
     1201 ccgttccttt ttaccgtccc attcctccac gggcaggagc gttccgtggg ggttccccac
     1261 ctcgccatcg acgatggccc cgtcgatggc caaccgggcg ccgctaccga aggtgtagcc
     1321 ggttttaagc gtcccgtgca aattgtccga atccgcgttt ttttgaaagc cgtccgtgag
     1381 cgagcgcccg gcgctggcgt aggcgtcgaa cccgccgacc cggtggcgga tgtctccctc
     1441 gtacaactgg gtgtaatagg accgaaactc gcttttgagg cgggtctggg gcttgtcccc
     1501 caccggccgt ttcgtcagca aatgaacgac gccgcccgtg gtgttggcgc cgtagaggac
     1561 cgaggccccg ccgcggacca cttcgatgcg gtcgatgtcc gtcaagggca tctgggacag
     1621 gtcgaaaaat tgattcgaga tgcccaacag cggcaagtcg tcgatcagga tctgttgctg
     1681 ggccgaagag ggcgaccccc gcaagcgcag cgtcgcgaag gacccggcgc cgccggattt
     1741 ttgcacgacg acggagggga acgggaccac gccctcgtcc agggccgtgg cgccgaggga
     1801 ttcccaatcg tccgcggtca ttcgggacac gttggtcggc aggcgtttgt ccggcgtggc
     1861 cgagcgggtg agggaaagaa agacgccgcc gtcctccgcc cggagaaagc cggccgcggc
     1921 cagggtcaca acgaggatga ggcgcttcat aacgactcct ttgttcattt tttggaatag
     1981 tgaaattgaa tgaatccggc ggttcccgaa tccccgggtc gaaccgaggc ctccaacccg
     2041 taggtggccg aaagttgctc ggaggataaa acccgggccg tggggcccag ggcgcggcac
     2101 tcgcccttat ccatcaacaa cgcccggggg caccacccgt aggccagatt gacgtcgtgg
     2161 aggatcgcga tcacggcgag ccgccgctcc cggcgcagcc gctcgatcac cgccaccaat
     2221 tccgcttggt ggcgggggtc caaatgggcc gtgggctcgt cgagcaacag gaggtggggt
     2281 tcctgggcca gggcccgggc caggaggacc cggcgccgct ccccgcccga caagcggtcc
     2341 accgggcgat gccgtagccc ggtcaaatcc gtctcctcca gggcgcggtg gacttcccgt
     2401 cgatcggcga tcgatcggcg cccccacaag ggtccgtggc aaaagcgccc gagcgaaacc
     2461 acgtcctcca ccgtcatgga aaaaagcgtc gacaggtcct ggggcaccca ggccactcga
     2521 cgggcgacct cccgggccga tagggattcc aaggaatcgt tccccaaacg gatctcgccg
     2581 gacccgggcc ccagcgttcc gcccagcagg cgcagcagcg tggatttgcc ggccccgttg
     2641 ggccccaaaa cggccaacag gtccccggcc tccatgtcaa aggaaattcc cttgagggca
     2701 aaggggcggt cgtcctccca gcgggggtag gcgaaggaaa ggtttttaac cgaaaggacg
     2761 gtcatcccag gtgtctccgt tcccgtcgga gcaggaccag aaaaaacggc gcgcccagca
     2821 gggccgtcac gaccctgctg
//
LOCUS       DAOQLS010000214         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20424_length_2838_cov_3.284226, whole genome shotgun sequence.
ACCESSION   DAOQLS010000214 DAOQLS010000000
VERSION     DAOQLS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20424_length_2838_cov_3.284226"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK362_04995"
     CDS             complement(<1..554)
                     /locus_tag="PK362_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74225.1"
                     /translation="MKRGVLFAAFLSGVAAVRADETAADLGAIAPGVQTTEIITLSTE
                     TETSAEKPAWLERAENLFQEGLAAEAAGDVRTARRRYSRTLKTLADQADDATVLDLRS
                     DIATFLNMADVGPLPRPGDCPPATEVKPVTQEELNAAPIVEPVVSSTKSYAIHLDPED
                     PLVKKFIGLYTGPLRSRFQEALNR"
     gene            complement(551..1801)
                     /gene="murA"
                     /locus_tag="PK362_05000"
     CDS             complement(551..1801)
                     /gene="murA"
                     /locus_tag="PK362_05000"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNC74226.1"
                     /translation="MDGFVLRGGRRLAGRVRVSGSKNAALPCLFATLLTDDPCELTNV
                     PDLQDIRTAVRLLERLGKKVSFQRGTVTVRRRGALQHKAPYDLVRRMRASAIVLGPLL
                     ARLGRAHVSLPGGCAIGARPINFHLKAFEALGAKVTVQEGYVAAAGRLRGKTLRLPFA
                     SVGATENVMMAAALARGRTVLKNAAREPEITDLADMLLGMGARVSGAGTSTIVIDGVD
                     RLGGVNHRVIPDRIEAGTLLIAAAITRGHLRVADARADHLSAVLAALRKAGLTVRADG
                     SDLVARWTRPLRPVSVTTKVYPGFPTDMQAQWMALMAVTRGKSVITESIFENRFLHAQ
                     ELQRLGARVDVRGSRATVEGVATLSGCPLMCSDLRAGAALVLAGLAARGVTKVLRVYH
                     LDRGYEALEKKLRAVGADIRRAPL"
     gene            complement(1807..2661)
                     /gene="prmC"
                     /locus_tag="PK362_05005"
     CDS             complement(1807..2661)
                     /gene="prmC"
                     /locus_tag="PK362_05005"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNC74227.1"
                     /translation="MTPTVADGLKRVTQRLTERGVPNADREALELVARAAAFSKDTLP
                     ARLREPLSAEAFVSLDQWTIRRAAGTPLAHLISEWDFLDFTVTVTPDVLIPRPETEGL
                     FDWAVRRMESDGGPAGLNRWVDCGTGSGALALAIARRWPNARVTAIDLSPAALAVARW
                     NARRWGLENRIEFQRADLLAGIGDGAVDGLVANLPYVEDGEWAGLAPEVRREPRAALL
                     GGPDGLDIIRRFVPQAARVLRPGGGVFLEVGRGQAPAVATLVSGYFRDVRTEKDFAGI
                     DRYVGGRR"
     gene            complement(2658..>2838)
                     /locus_tag="PK362_05010"
     CDS             complement(2658..>2838)
                     /locus_tag="PK362_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNC74228.1"
                     /translation="ERTEKIRTYNFPQDRVTDHRLNQNFHNLPRILEGGLDDMFQALA
                     DAEKAQKLAALEAPQ"
BASE COUNT          491 a          968 c          937 g          442 t
ORIGIN      
        1 atccggttca acgcctcttg aaaacgactc cgcaggggcc cggtgtagag gccgatgaat
       61 ttcttgacca ggggatcctc gggatccaaa tggatggcgt agctcttggt ggaagagacc
      121 accggctcga cgatcggagc ggcgttcaat tcctcttggg tcacgggttt gacttccgtg
      181 gccggagggc agtccccggg ccggggaagc gggcccacgt ccgccatgtt caagaaggtg
      241 gcgatgtcgc ttcgcaaatc gagaaccgtc gcgtcgtccg cctggtccgc cagggttttc
      301 aacgtgcggc tgtaccggcg ccgggccgtc cgaacgtccc cggcggcttc ggccgccaga
      361 ccctcctgga acaggttttc ggccctctcc agccaagccg gtttttccgc cgaagtctcg
      421 gtttccgtcg acaaagtgat gatttcggtc gtctgaacgc ccggggcgat ggccccgaga
      481 tcggccgcgg tctcatcggc ccggacggcc gcgacgcccg acaagaaagc cgcgaaaagg
      541 accccgcgct tcacaggggc gcccgccgaa tatcggcgcc cacggcgcgc agttttttct
      601 ccagggcctc gtatccccgg tccaggtggt acacccggag aactttggta acgccccggg
      661 cggccaagcc ggccaacacg agcgccgcgc cggcccgcaa atcggaacac atgagggggc
      721 accccgacag ggtcgccacg ccctccaccg tcgcccggga accgcgaacg tccacccggg
      781 cgcccaaccg ctggagctcc tgggcgtgga ggaatcgatt ttcaaaaatg gattcggtga
      841 tcaccgattt gccccgggta acggccatga gcgccatcca ctgggcctgc atgtcggtgg
      901 ggaaacccgg atacaccttg gtggtgacgg acacgggacg aagggggcgg gtccagcgcg
      961 ccaccaggtc cgacccgtcg gcgcgcaccg tcaaaccggc ttttcgaagc gccgcgagga
     1021 ccgccgacaa gtggtcggcc cgggcgtcgg ccacccggag gtggccccgg gtaatggccg
     1081 cggcgatcaa cagcgtcccg gcctcgatcc gatcgggaat cacccggtgg ttcacgccgc
     1141 ccaaacggtc cacgccgtcg atgacgatgg tgctcgtccc cgcgccggac acccgggccc
     1201 ccatgccgag gagcatgtcg gccaaatcgg tgatttcggg ttcccgggcc gcgttcttga
     1261 gcaccgtccg gccccgggcc agggcggcgg ccatcatgac gttttccgtg gcgcccacgc
     1321 tggcgaaggg gagtcgaagg gttttcccgc gcaaacggcc ggcggcggcg acgtatccct
     1381 cctgaaccgt cactttggcg cccagggctt caaaagcttt aagatggaag ttgatggggc
     1441 gggcgccgat ggcgcaaccg ccgggaaggg acacgtgggc gcgccccaag cgggccaaca
     1501 aggggccgag cacgatggcg ctggcgcgca tccggcggac caaatcgtag ggcgctttgt
     1561 gctggagggc gccgcgccgc cggaccgtca ccgtcccccg ttgaaaagac accttcttcc
     1621 ccaatcgctc cagcaggcgg acggcggttc gaatgtcctg cagatccggc acgttggtca
     1681 gttcgcaggg gtcgtcggtc aggagcgtgg cgaagaggca agggagcgcg gcgtttttgg
     1741 accccgacac ccggacccgg cccgccagcc gacggccccc ccgaagaaca aacccgtcca
     1801 atcgcatcat cggcgccccc ccacataccg gtcgatgccg gcgaaatctt tttccgttcg
     1861 cacgtcccgg aagtaaccgc tcacgagcgt cgccacggcg ggcgcctgcc cccggcccac
     1921 ttccaggaac acccccccgc ccggacgaag gacccgggcg gcctggggaa caaaccgacg
     1981 aatgatgtcg agtccgtcgg gccctcccaa caaggcggcc cggggctccc gccgcacttc
     2041 gggcgccaac ccggcccatt cgccgtcttc cacgtagggc aagttggcga ccagaccgtc
     2101 cacggcgccg tccccgatcc cggccagaag atcggcccgc tgaaattcga ttcgattttc
     2161 gaggccccat cggcgggcgt tccaccgggc cacggccagg gcggccgggg aaagatcaat
     2221 ggccgtcacc cgggcgtttg gccaccgacg ggcgatggcc agggccaggg ccccggaacc
     2281 ggtgccgcaa tccacccaac gattcagtcc cgccgggccc ccgtcgctct ccattcgacg
     2341 aacggcccaa tcaaagagcc cttcggtctc gggccggggg atgaggacgt cgggggtcac
     2401 cgtcaccgtg aaatccagaa aatcccattc gctgatcaag tgggccaggg gggttcccgc
     2461 ggcccggcga atcgtccatt gatcaaggga aacaaaggcc tcggcgctca ggggttcccg
     2521 caggcgggcg ggcagggtgt ccttggaaaa agcggcggcc cgggccacca actccagggc
     2581 ttcccgatcg gcgttgggaa cgccgcgctc cgtcaaccgc tgggtgaccc gctttaatcc
     2641 gtcggcgacg gtgggggtca ttggggagcc tcgagcgccg ccagcttctg ggctttttcc
     2701 gcgtcggcga gggcctggaa catgtcgtcc aatccaccct ccaaaatgcg cggcagattg
     2761 tgaaaattct ggttcaggcg gtgatcggtc acccggtcct ggggaaaatt gtaggtgcgg
     2821 attttttcgg ttcgttcg
//
LOCUS       DAOQLS010000215         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20511_length_2833_cov_3.853492, whole genome shotgun sequence.
ACCESSION   DAOQLS010000215 DAOQLS010000000
VERSION     DAOQLS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20511_length_2833_cov_3.853492"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2342)
                     /locus_tag="PK362_05015"
     CDS             complement(<1..2342)
                     /locus_tag="PK362_05015"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC74229.1"
                     /translation="MKKLFGAEALIGLTLTLLIGLLAYLQPGFTESLELKLYDLRLHL
                     SPSAAPSDDIRMVAIDDASIEAVGRWPWARGAVAELLRRVAAGKPKVIGLDIMYVDPD
                     ANQGLEAVRALKKEFSDLLAKRRKSPAPKGRGGKSKVGANALDDLESFELSLEDTEES
                     LDNDAQLAGALAQTKNVVLPLTFATLDKPLVDEPPESMERMAARALLQSQIANDRDPR
                     VTEGFQPLLPIPDFAKNVTGMGHCNIFSDIDGTVRRDTVVIKYAGQYHPSLALEMARI
                     ALNVPTNKLSVNLGTELRLGDRRIPLDSESRFFIKFQGPFADLKKVSAVDVLRDEGGV
                     PPEAFKNKIVLIGLTALGVGNVFVTPAESTHQFNGVILSALQNILDGDFISRPPWAGT
                     AEALWILLLGLVATFLWPHLKAKGGLAVFLVLLAATVGTGTFLFVARGWWLKIFYPLA
                     LILLSYGLVTVRRFFFAEGRKEFVEAQSIETNKMLGQSFQGQGLLDMAFAKFQLVPVD
                     EEMKGVLYNLALDFERKRQFNKAAVVYEHIAKADPNFKDIKERAKSMKQAGETMIAGP
                     GLGGGKEGGTIAIAGGAAKPTLGRYEIQKELGRGAMGVVYLGKDPKINRSVAIKTLRF
                     DDDMDAETAKFTKERFFREAESAGTLNHPHIIRIFDAGEDNEISFIAMELLEGEDLKK
                     YVEKDHLLPVPQVLEYVAQIADALDYAHQNGVVHRDVKPANIMRLKDGSLRVTDFGIA
                     RITASSKTATGTVMGTPSYMSPEQLSGKKVDGRSDLFSLGV"
     gene            complement(2371..>2833)
                     /locus_tag="PK362_05020"
     CDS             complement(2371..>2833)
                     /locus_tag="PK362_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC74230.1"
                     /translation="MFLKRHVDILSFFTDDQLRRVTAEIDRQTYNKGQTVVFQGEISH
                     NFHIIKRGKMQVFSKSGGDRALVAELGPGDFFGEMSLLDSTTASATIRAAEDGSEILM
                     ISHDTFKQLLREFPALELALRDKVAERQRQRHAALQAKKPGDAPAGGGSPL"
BASE COUNT          528 a          816 c          913 g          576 t
ORIGIN      
        1 accccgaggg aaaacaagtc cgaccgcccg tcgacttttt tgccggacaa ttgctcgggc
       61 gacatgtagc tcggggtccc catcacggtc cccgtcgccg tcttggagga ggcggtgatg
      121 cgggcgatgc cgaaatcggt gacgcgaagg gaaccgtcct tgaggcgcat gatgttggcc
      181 ggtttcacgt cccggtggac aacaccgttt tggtgggcgt aatccagagc gtcggcgatt
      241 tgggcgacgt attccaacac ctgggggacc ggcaacaaat ggtctttttc gacgtatttc
      301 ttgaggtcct ccccctccag caactccatg gcgatgaagg aaatttcgtt gtcctccccc
      361 gcgtcgaaaa tccggatgat gtgggggtgg ttcagcgtcc cggcggattc ggcctcccgg
      421 aaaaaccgtt ccttcgtgaa tttggcggtt tcggcgtcca tgtcgtcgtc aaaacgaagg
      481 gttttgatgg ccacggaccg gttgatcttg gggtctttgc cgagataaac gacccccatg
      541 gcaccgcggc ccagctcttt ttgaatctcg taacggccca gggtcggttt ggcggcgccg
      601 cccgcgatgg cgatggtgcc gccctccttt cccccgccca aaccgggccc ggcgatcatg
      661 gtttcgccgg cctgtttcat ggatttcgcc cgttccttga tgtccttaaa attcggatcg
      721 gctttcgcga tgtgttcgta gaccaccgcg gctttgttga attggcgttt ccgctcaaaa
      781 tcgagggcca gattgtagag gacgcccttc atttcctcgt ccaccgggac cagctggaat
      841 ttcgcgaagg ccatgtcgag gaggccctgg ccttggaagg actggccgag cattttgttg
      901 gtctcgatgc tttgggcttc gacgaattcc ttgcggccct ccgcaaagaa gaagcggcgg
      961 acggtgacca gtccgtagga aagcaggatc agggccaacg ggtagaaaat cttcaaccac
     1021 cagccccggg cgacaaagag gaaggtcccc gtgcccacgg tggcggcgag cagaacgagg
     1081 aaaacggcca atccgccctt ggctttcagg tgcggccaca ggaaggtggc gacaagcccg
     1141 agcaagagaa tccagagggc ttcggcggtc cccgcccagg gggggcgcga aataaaatcc
     1201 ccgtccaaaa tgttttgcaa ggccgagagg atgacgccgt tgaactggtg ggtcgattcg
     1261 gccggggtga cgaacacgtt ccccacgccc agggccgtca ggccgatgag gacgattttg
     1321 tttttaaagg cttcgggcgg cacccccccc tcgtcgcgca acacgtccac cgcggagacc
     1381 ttcttgagat cggcgaaggg gccctggaat ttgatgaaaa accggctttc cgaatccagg
     1441 ggaatgcgtc ggtcccccaa tcgaagttcg gtgcccagat tgaccgaaag tttattggtc
     1501 gggacgttca gggcgatgcg ggccatctcg agcgccaaag aggggtggta ttggccggcg
     1561 tatttgatca cgacggtgtc ccgccgaacg gtcccgtcga tgtcggagaa gatattgcaa
     1621 tgccccatcc cggtgacgtt cttggcgaag tccgggatcg ggagaagcgg ttgaaaaccc
     1681 tccgtgaccc gggggtcccg gtcgttggcg atttgggact gcagcaacgc ccgcgcggcc
     1741 atgcgttcca tcgactccgg cggttcgtcc accaggggtt tgtccaaggt ggcgaaggtg
     1801 agcggcagga cgacgttctt cgtctgcgcg agggcgccgg ccagttgggc gtcgttgtcc
     1861 aacgattcct cggtgtcttc caggctcaat tcaaaagact ccagatcgtc caaggcgttc
     1921 gccccgactt tcgatttgcc gccgcgtccc ttgggggccg gcgattttcg ccttttcgcg
     1981 aggagatcgg aaaattcttt tttgagggcg cgaacggctt ccaacccctg gttggcgtcc
     2041 ggatcgacat acatgatgtc gagaccgatg accttgggtt tccccgccgc gacccgtcga
     2101 aggagttcgg ccaccgcgcc ccgggcccag ggccaacggc cgacggcctc gatggaggcg
     2161 tcgtcaatgg cgaccatccg gatatcgtcc gagggagcgg ccgacggcga caggtgaagg
     2221 cgcaaatcgt aaagttttag ttccagggat tcggtgaacc cgggttggag ataggccagg
     2281 aggccgatga ggagggtgag ggtcagcccg atgagggctt cggcgccaaa aagttttttc
     2341 acgggagggg ccccgacgcg atcggtcccc tcacaacggc gaacccccgc cggcgggggc
     2401 gtcgccgggt ttcttggctt gaagggcggc gtggcgttgg cgctggcgtt cggccacttt
     2461 gtcccgcagg gccagctcca gggccggaaa ttcccgaagc aactgcttga acgtgtcgtg
     2521 ggagatcatg agaatttccg acccgtcttc cgccgcccga atcgtggcgc tggcggtcgt
     2581 ggagtccaag agggacattt caccgaaaaa atccccgggg cccaattcgg ccaccagggc
     2641 ccggtcgccg ccggatttgg agaaaacctg cattttgccg cgcttgatga tgtgaaaatt
     2701 gtggctgatc tccccttgga acacgacggt ctggcccttg ttgtaggttt gccggtcgat
     2761 ctcggccgtg acgcggcgca gctggtcgtc cgtgaaaaag gacaagatat cgacgtggcg
     2821 cttcaagaac atc
//
LOCUS       DAOQLS010000216         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20512_length_2833_cov_3.733621, whole genome shotgun sequence.
ACCESSION   DAOQLS010000216 DAOQLS010000000
VERSION     DAOQLS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20512_length_2833_cov_3.733621"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1969)
                     /locus_tag="PK362_05025"
     CDS             complement(<1..1969)
                     /locus_tag="PK362_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC74231.1"
                     /translation="MRALLKYCVERPLGVLAVTTGLAVIGLFAWRSLPQELMPDLRYP
                     QLSVVTLLPNASPEEVENLVTKPIEQILGTVKNVRGVESFSKEQVSLVNIQFRWDTDM
                     DAALLWVQEKLGLVQDSLPLEAKKPEVTRYNPFERPVLLIAVTGSLPAEDLDHLVESR
                     VRPTLEKTLGVSGIEVSGGLEREVRVDLDAQQLLAHRLTLQDVADALKRRNVSRSAGS
                     AAEGLFEYPITVTGAYDQIKAIGETVVKTDGAAGRPGEGGLLRLSSVGQVKDGFREKT
                     SHARYDGRDNIAVAVFKRSEAYPLDVARDVRESLADLRRQLPGDVHLDVIYDQSVFIR
                     EGISDVFGNVVVGGLLAYFILWAFLRSHRRAMIVGLTIPLALLLTVAVFWKMDLTLNL
                     LTLGGLALGVGMLVDAAVVIIENISRHRDLGKSLKDSILDGGEEVGGAVTFSVLTILA
                     AFAPIPFAAVGVAQKVFTPIAVAVMLSHAMSLVVGFTFVPAIGILFLESGRGREWIPP
                     GVAVFVRDLWAVWNKQWTRLKDRARQTAFYRWRWEQWAARPGHWYESALQFAVRHPRR
                     TVELAFLCTLANGVGLFFIRRESMPDVDHNQIVMRVTLPAGTRLEVTDRVMGSIEKVL
                     ADLPEIAHRNVVVGSSGGNLLVSPAGAMPLQV"
     gene            complement(1979..>2833)
                     /locus_tag="PK362_05030"
     CDS             complement(1979..>2833)
                     /locus_tag="PK362_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC74232.1"
                     /translation="LQFGAPGRISRFTVEKGQFVKKGALLAALDQAEAKNALDVAQLE
                     YEKAAVKYFKDQTIDRLTYEQAKARYKQAQLEADKTTIRAAHDGYLVEKWVNEGEHAD
                     PGTVIGKLMDKSRVFIEMDLSEDDIQHLKPGQKVAVTVDAVPDFKEDGTVLSVTPYLK
                     GDTRSFGVKVDLPANPQEKLSPGMFARCTVRRYEKVGALTVPLEAGAEMQQKTMRLFV
                     VDEKNAAHARTADILFMDEGIVEVSGVTEGELVILNPGTDLEDGATVRVMDVFDPAAH
                     TESPVAPG"
BASE COUNT          557 a          888 c          851 g          537 t
ORIGIN      
        1 ccacctgcag gggcatcgcc ccggcgggcg agaccaggag attccctccc gaggacccga
       61 cgacgacgtt gcggtgggcg atttccggca ggtccgccag gaccttctca atggaaccca
      121 tcacgcggtc cgtcacctcc agccgggttc cggcggggag ggtgacgcgc atgacgattt
      181 ggttatggtc cacgtcgggc atggattccc ggcggatgaa aaagagcccc accccgttgg
      241 cgagggtgca caagaaggcc aattccacgg tccgccgggg atgacgcacg gcgaattgca
      301 acgcgctctc gtaccaatgc ccgggccggg cggcccattg ttcccaccgc caacggtaaa
      361 aagcggtttg acgagcccgg tccttcaacc tcgtccattg tttattccac accgcccaca
      421 aatcccgaac aaaaaccgcc acccctgggg gaatccattc ccgcccgcgg ccgctttcca
      481 aaaaaagaat gccgatggcc ggcacgaagg tgaaccccac caccagggac atggcgtggc
      541 tgagcatgac ggccacggcg atgggcgtga aaaccttttg cgccactccg acggccgcga
      601 aaggaatcgg agcgaaggcg gccaaaatcg ttaaaacgga aaaggtgacc gcgcccccca
      661 cctcctcccc gccgtccaga atgctgtctt tcaagctttt ccccaggtcc cggtggcggg
      721 aaatgttctc aatgatgacg acggcggcgt ccaccagcat ccccacgccc agggccaagc
      781 cccccagggt cagcaggttc aaggtcaaat ccattttcca gaagacggcc acggtgagca
      841 agagcgccaa ggggatggtc agacccacga tcatcgcccg ccgatggctc cgtagaaaag
      901 cccaaagaat aaaataggcg agcaggccgc ccaccacaac gttgccgaac acatcggaaa
      961 taccttcccg gataaaaacg gattgatcgt agatcacgtc cagatggacg tccccgggca
     1021 gttgccgccg cagatccgcc agagactcgc gcacgtcgcg ggccacgtcg agggggtaag
     1081 cttccgagcg tttaaacacc gccacggcga tgttgtcccg gccgtcgtat cgcgcgtggc
     1141 tcgtcttctc ccgaaagccg tctttcactt gccccacgct cgacagtcgg agaaggccgc
     1201 cttcgcccgg acggccggcg gcgccgtcgg ttttaacgac ggtttccccg atggctttta
     1261 tttgatcgta ggcgccggtg acggtaatgg gatattcgaa aagaccttcc gcggccgagc
     1321 ccgccgaccg ggaaacgttg cggcgtttta aggcgtcggc cacgtcctgg agcgtcaatc
     1381 gatgggccaa aagctgttgg gcgtccagat ccacgcggac ctcccgttca aggccgccgc
     1441 tcacctcgat gccggacacc ccgagggttt tttccagagt cggtcgcacc cggctttcca
     1501 ccaaatggtc aagatcctcc gcggggagcg aaccggtgac ggcgattaaa aggaccggtc
     1561 gctcgaaggg gttgtagcga gtgacttccg gtttcttggc ttccaaggga agggaatcct
     1621 gaaccaagcc caatttttcc tgaacccaaa gaagagcggc gtccatgtcg gtgtcccagc
     1681 ggaattggat attgaccagg gaaacctgtt ccttggagaa ggactccacc ccccggacgt
     1741 ttttgaccgt ccccaggatt tgttcgatgg gtttggtgac gaggttttca acttcttcgg
     1801 ggctcgcgtt gggaaggagg gtaacgacgg acagttgcgg gtagcggagg tcgggcatca
     1861 attcctgggg aaggctccgc caggcgaaga gaccgatgac cgccagaccc gtggtgacgg
     1921 ccaagacgcc caggggccgc tccacgcaat acttaaggag ggcgcgcacg ggcgaccgtc
     1981 agccgggggc gacgggggat tcggtgtggg ccgctgggtc aaaaacgtcc atgacccgga
     2041 ccgtggcgcc gtcttccaaa tccgttcccg ggttgaggat caccaattcc ccttcggtca
     2101 cgccggaaac ctccacgatg ccttcgtcca tgaataggat gtccgccgtc cgggcgtggg
     2161 ccgcgttttt ctcgtccacc acgaaaaggc gcatggtctt ttgctgcatt tcggcgccgg
     2221 cttccaaggg gacggtcagg gcgccgactt tttcgtagcg gcgcaccgtg catcgggcga
     2281 acattccggg gctcagtttt tcctggggat tggccggaag atccaccttg acgccgaagg
     2341 accgcgtgtc gcctttcaga tagggcgtga ccgaaagcac cgtgccgtct tccttaaaat
     2401 cggggacggc gtccaccgtg acggcgactt tctgccccgg cttcaaatgt tgaatgtcgt
     2461 cctcggacaa gtccatttcg atgaacaccc ggcttttgtc catcagtttc ccgatgaccg
     2521 tacccggatc ggcgtgttcc ccttcgttca cccacttctc caccaagtac ccgtcgtggg
     2581 ccgcccgaat ggtcgttttg tccgcctcca actgggcctg cttgtaacgg gccttggcct
     2641 gctcgtaggt aagacggtcg atggtttgat ccttgaaata tttgacggcg gctttctcgt
     2701 attccagttg ggccacgtcg agcgcatttt tggcctcggc ctggtccaag gcggccagga
     2761 gggcgccctt tttcacgaat tggccctttt ccaccgtaaa gcgggagatc cggcccgggg
     2821 cgccgaactg cag
//
LOCUS       DAOQLS010000217         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20764_length_2813_cov_3.530819, whole genome shotgun sequence.
ACCESSION   DAOQLS010000217 DAOQLS010000000
VERSION     DAOQLS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20764_length_2813_cov_3.530819"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="PK362_05035"
     CDS             complement(<1..2154)
                     /locus_tag="PK362_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC74233.1"
                     /translation="MPRQGKMLASLARVAERLRSVEAALEHVDGPLTEEDAWEEDAKK
                     WPTAWAGLEGEELYREALTVAKTVSPAGERGLARARRLLDPFVGRFRARFRAAGWLGF
                     DDLLRGARDLLANHPEVRREVKARYGAILVDEFQDTDPLQGETILFLAEAPDSEAAAW
                     KDVSLAPGKLFIVGDPKQSIYRFRGADIRAYEAFVETVLAQGGRRCDLQMSFRTHEGI
                     VDPVNRLFGGLMVETPGLQPLYRPLLPRPVDPPGAAGDRGVELILFDDPALSSARAAQ
                     AAEARWIARWIVEHVGPASSPWRLGDVALLFRSAAPLTVYMEALKSAGVPYLVESDRS
                     FYGTPEAMDFLNLLRCLADPDDRVAQIGLLRSPLVALEDRDLLVLSEADAWAGVARPA
                     GLADEAWEALREFHRWTGSLRSAAAGDSLGVLAQRILRESPILAAAAAAYHGEQSVSN
                     LYKLARLAGEAHQTRGETLGGFVRWLSRAVDEGVEEGESPLGEENVDAVRLLTVHKAK
                     GLEYKIVFLPNLAGKVQGGARNPPAVRVDWAGDRAGHRLIERKWADAGMAFLEADERR
                     REAGEAVRVFYVAATRARERVFLLGQQKEAPGTFMEMLRRGVTESDGVWSFADGRRLA
                     VIRVGAAAGGDPRWPRAEAPRECLASPARSRAWERRRRDFAVAPRERLFVRPSDLRTE
                     NPATPQLPSEGDDHKTGAAALGELCHAVLAEWDFEG"
     gene            complement(2058..>2813)
                     /locus_tag="PK362_05040"
     CDS             complement(2058..>2813)
                     /locus_tag="PK362_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC74234.1"
                     /translation="VEAGAGTGKTTLLTDRLMFLLLAGGPEGEGLPVTRLVALTFTDK
                     AAGEIKVRLAERLGDLLLRLEGGVLPSARRDRTDHWVREARADFNATDDRLRAMATEA
                     LRDLDRAPLGTIHGFCRTILRLYPLEAGLSPGFRVDNGEAFEALFREEWARWLEEELV
                     PGRPSESRWRDVLPHVGLGDLAALARSLPSGPRPRGDRNRFCRGWPRCGGTWKPCPRG
                     SPCPVRGRCWRPSPGWRNGCVRWRRPWSTWTAR"
BASE COUNT          477 a         1035 c          890 g          411 t
ORIGIN      
        1 gccctcaaaa tcccattccg ccaacaccgc gtgacacaac tcccccaagg ccgcggcccc
       61 ggttttatga tcgtcccctt ccgagggaag ttgaggcgtc gcggggttct ccgtcctcaa
      121 atcggaggga cgaacaaaca agcgttcccg gggtgcgacg gcgaaatccc gtcggcgtct
      181 ttcccaggcc cggctccggg ccggcgacgc cagacattcc cggggagcct ccgcccgggg
      241 ccagcgggga tccccgccgg cggcggcccc gacccgaatc acggccagtc gccgcccgtc
      301 ggcgaaggac caaaccccgt cggattccgt cactccccgg cggagcattt ccataaacgt
      361 tcccggggcc tctttttgtt ggccgagaag gaaaacccgc tcccgggccc gggtggcggc
      421 gacgtaaaaa acccggaccg cttcgcccgc ttcccgacga cgttcatcgg cttccaagaa
      481 ggccattccc gcgtcggccc atttccgctc gatcaggcgg tgcccggccc ggtccccggc
      541 ccaatccacc cggaccgcgg gcgggttccg ggcgccgcct tggactttcc cggcgaggtt
      601 cggcagaaat acaattttgt attccaaccc cttggctttg tggacggtca agaggcgcac
      661 cgcgtcgacg ttttcctcgc ccaagggact ttccccctcc tccacccctt cgtcgacggc
      721 ccgggacaac caccgcacga acccgcccag agtctccccc cgggtttgat gggcctcccc
      781 ggccagccgg gcgagcttgt agaggttcga cacgctttgt tcgccgtggt aagccgccgc
      841 ggccgcggcg aggatcggcg actcccggag aatccgttga gccaacacgc cgagggaatc
      901 cccggccgcg gccgaccgaa gggatccggt ccaccgatga aattcccgaa gggcctccca
      961 cgcttcgtcg gcgagccccg ccggtcgagc gacgcccgcc cacgcgtccg cctccgaaag
     1021 gaccagcaga tcgcgatcct ccagggcgac caacggtgac cggaggaggc cgatttgagc
     1081 gacccggtcg tcggggtccg ccaaacaccg gagaagattg agaaaatcca tggcttccgg
     1141 ggtgccgtag aagctccgat cgctttcgac cagataaggg acgccggcgg atttgagggc
     1201 ctccatgtac accgtgaggg gcgccgcgct gcggaaaagg agggccacgt cgcccaaccg
     1261 ccaggggctc gaggcgggcc ccacgtgctc cacgatccac cgggcgatcc atcgggcctc
     1321 ggccgcctgg gccgcccggg cggaggacag ggccgggtcg tcaaagagga ttagttccac
     1381 cccccgatcc cccgccgccc ccggggggtc gaccggacgc ggaagcagcg ggcgatacag
     1441 gggctgaagg cccggcgttt cgaccatcag gcccccgaag aggcggttga cggggtcgac
     1501 gattccctcg tgggtgcgga agctcatttg aaggtcgcac cggcggcccc cctgggccag
     1561 cacggtttcg acgaaggcct cgtaggcccg aatatcggcc ccgcggaagc ggtaaatgga
     1621 ttgtttcgga tccccgacga tgaaaagttt tcccggggcc agggagacgt ccttccaggc
     1681 cgcggcctcg ctgtcggggg cctcggccaa aaacaggatc gtctcccctt gaagggggtc
     1741 cgtgtcctga aattcatcca caaggatcgc cccgtaacgc gctttcacct cccgtcgaac
     1801 ttccgggtgg ttggccagaa ggtcccgggc gcctcggagc agatcgtcga agcccagcca
     1861 tcccgcggcg cggaaccggg cccggaaccg gccgacaaag gggtccagca agcggcgggc
     1921 ccgggccaaa ccccgctcac cggccggcga aacggttttg gccaccgtca gggcctcgcg
     1981 gtagagttct tcgccttcca aaccggccca agccgtcggc catttcttgg cgtcttcctc
     2041 ccaggcgtct tcctcggtca acgggccgtc cacgtgctcc agggccgcct ccaccgaacg
     2101 caaccgttcc gccacccggg cgagggacgc caacatcttc ccctgacggg gcacgggctt
     2161 cccctcgggc agggtttcca cgtcccgccg caacgcggcc aaccgcggca aaaacgattc
     2221 cggtcgcccc gggggcgtgg cccgctgggc agggacctcg cgagggcggc caggtcgccc
     2281 aaccccacgt gaggcaatac gtccctccac cggctttccg acgggcggcc gggaaccaat
     2341 tcctcctcca gccaccgggc ccactcctcc cgaaacagcg cttcgaaggc ctcgccgttg
     2401 tccacccgga acccgggcga caaccccgcc tccagcgggt aaagccggag aatcgtccgg
     2461 caaaaaccgt ggatggttcc caggggggcc cggtccaaat cccgcagggc ttccgtcgcc
     2521 atcgcccgca atcgatcatc ggtggcgttg aagtccgccc gggcctcccg aacccaatga
     2581 tcggtgcggt cccgccgggc ggaaggcagg acgccgccct ccagtcgaag caggaggtcc
     2641 cccaggcgct cggccaggcg gactttgatt tccccggcgg ccttgtcggt gaacgtcagg
     2701 gccaccaacc gcgtgacggg aaggccctcg ccctcgggtc cccccgccag gaggagaaac
     2761 atcagccgat ccgtgagcag ggtggtttta cccgtaccgg cgccggcctc cac
//
LOCUS       DAOQLS010000218         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20845_length_2806_cov_4.640858, whole genome shotgun sequence.
ACCESSION   DAOQLS010000218 DAOQLS010000000
VERSION     DAOQLS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20845_length_2806_cov_4.640858"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="PK362_05045"
     CDS             <1..1036
                     /locus_tag="PK362_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HNC74235.1"
                     /translation="GVLASALTSSRVPHAYLFVGPEGVGKAKAARLWAQILLCEAPVS
                     PVQACGQCVGCRKAVAGGHPDLLSVDFERQAALLKEPLEKQKSIKIDTVREMEHTLRL
                     KPSEARVKVALLDPADALVDAAAHALLKILEEPPAATHLVLIAREATALLPTIRSRCH
                     LVRFRPLDQGTLAEIVRGLKPGVSEAETRTAATVAEGSVARALAAIDGSGALDFDWTS
                     VPLSELLAWCEGFGNPRLGRSAAERLVESLMAREQESLRRGEGSAEALRESLTALHRL
                     RQNAHVTLTLQTLLLGLRRRAREKAEARPSRDGLAPLGSAAAGTGHEPRASTGGLDPG
                     PRPGTSKEPL"
     gene            1033..2559
                     /gene="metG"
                     /locus_tag="PK362_05050"
     CDS             1033..2559
                     /gene="metG"
                     /locus_tag="PK362_05050"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNC74236.1"
                     /translation="MKPYYLTTPIYYANDVPHIGHAYTTIAADVLARWKRLSGARVHF
                     LTGTDEHGAKIAQAAGAAGQTPRAYLDRVVAKFQELWTRLTVTPDDFLRTSDARHIGV
                     VQKIFEKLLKDGHIYLGSYEGWYCVPDETFWSESELKEGKCPACGRTVERLKEESYFF
                     KLSAFEAPLLAHFEKHPEFLQPKSRAPEIVQFVKMGLRDLSVSRTKVAWGVPVPSNPA
                     HTVYVWFDALINYISALGYDPAAPGALYKEFWPADVHLVGKEIFRFHAVIWPAMLLAL
                     GVPLPRTVFAHGWWTVDGEKMSKSRGNVVDPHRMADEFGVDAFRYFLLREVPFGADGD
                     FSAKALLGRYNAELANALGNLLNRVLNLLEKNFDSALLPAGTGDLLPENADWARAYND
                     ALDRTAFHEALEVAFGLVNRANKYAEDTAPWKMVKTDAPRTHAVLTEMARVLKVAAVP
                     LPPFMPTVTEEMWRQLGEPRPLGAEAARALAGGKIQFDPGQKIRKGNPLFPRKEAPKP
                     "
     gene            2556..>2806
                     /locus_tag="PK362_05055"
     CDS             2556..>2806
                     /locus_tag="PK362_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC74237.1"
                     /translation="MTPPVAIDTHAHLADEKFDADRDEVLKRAWDAGLSVVVEIGEEE
                     SQWPKARALAEAHPGRVYWTAGYHPYYAIKADDEKFDADR"
BASE COUNT          505 a          959 c          868 g          474 t
ORIGIN      
        1 gggcgtcttg gcctcggcct tgacgtcaag ccgcgttccc cacgcctatc tcttcgttgg
       61 cccggaaggc gtcggcaaag ccaaggccgc ccggctctgg gcccaaattt tgttgtgcga
      121 agccccggtt tccccggtcc aagcctgcgg tcaatgcgtg ggctgccgta aggccgtggc
      181 gggcggccac cccgatctcc tttccgttga ctttgagcga caggcggccc ttctgaagga
      241 gcccctggag aaacaaaaat ccatcaaaat cgacacggtt cgggaaatgg agcacaccct
      301 ccgcctaaaa ccctccgaag cccgggtcaa agtggctctg ttggacccgg cggacgccct
      361 cgtggacgcg gcggcccacg cgcttctcaa gattttggag gaaccccccg ccgcgacgca
      421 cctggttttg atcgcccggg aagccacggc cctcttgccg acgatccgtt cccggtgcca
      481 cctggtccgc tttcgtccct tggatcaagg gaccctggcc gagattgtcc gggggcttaa
      541 gcccggcgtt tccgaagcgg aaacccggac ggccgccacg gtggccgaag gatccgtggc
      601 ccgggcgctg gccgccatcg acgggagcgg cgcgctggat ttcgattgga cgtcggttcc
      661 cttgagcgag cttttggcgt ggtgcgaagg tttcggcaat ccccgactcg gccgctcggc
      721 ggcggaacga ctggtggagt ccctgatggc ccgggaacag gaatccctgc gccggggcga
      781 gggatccgcc gaggccctgc gggaaagttt gacggccctg caccgcctgc ggcaaaacgc
      841 ccacgtcacg ctcactttgc aaaccctgct cctggggctc cgccgccggg cccgggagaa
      901 ggcggaggcc cgaccgtcaa gggacggcct cgcccctctg ggctcggccg ccgctggcac
      961 gggacatgaa ccccgcgcca gcactggggg acttgacccc gggccaaggc ccgggacgtc
     1021 taaggagccc ctgtgaaacc ttattacctg acgaccccca tctattacgc caacgacgtg
     1081 ccccacatcg gccacgccta caccaccatc gccgccgacg tcctggcccg ctggaaacgc
     1141 ctgtccggcg cccgggtcca tttcttgacg gggaccgacg aacacggcgc caaaatcgcc
     1201 caggccgccg gcgccgccgg gcaaaccccc cgggcctacc tggaccgggt ggtggccaaa
     1261 ttccaggagc tttggacccg cctgaccgtc actcccgacg attttctccg aacctccgac
     1321 gcccgccaca tcggcgtggt gcaaaaaatc tttgaaaagt tgttgaagga cggacacatt
     1381 tacctcgggt cctacgaggg gtggtactgc gtccccgacg aaaccttctg gtccgaatcg
     1441 gaattgaagg aagggaaatg ccccgcctgc ggccggaccg tggagcgttt gaaggaggag
     1501 agctatttct tcaaactctc cgcctttgag gcgccgctcc tggcccattt cgaaaaacat
     1561 cccgaattcc tccagccgaa gtcccgggcg ccggaaatcg ttcaattcgt caaaatgggc
     1621 cttcgggatc tctcggtgag ccgcaccaaa gtggcctggg gggttcccgt gccgtccaac
     1681 cccgcccaca cggtttacgt gtggttcgac gcgctcatca actatatttc ggccctgggg
     1741 tacgaccccg cggcgccggg cgcgctttac aaggagtttt ggccggcgga cgtccatctg
     1801 gtgggaaagg aaatcttccg tttccacgcc gtgatctggc ccgccatgct cctggccctg
     1861 ggcgttcccc tgccccggac ggtgttcgcc cacgggtggt ggacggtgga cggggaaaaa
     1921 atgtccaagt cccggggcaa cgtggtggac ccccaccgca tggccgacga attcggggtg
     1981 gacgccttcc gttatttcct ccttcgagaa gtgcccttcg gcgccgacgg ggacttttcc
     2041 gccaaggcgc tcctggggcg ctacaacgcc gaactggcca acgccctggg caacctgctc
     2101 aaccgcgtgt tgaacttgtt ggaaaagaat tttgactcgg cgctccttcc cgcgggcacg
     2161 ggggacctgt tgcccgaaaa cgcggattgg gcccgggcct acaacgacgc tctcgaccgc
     2221 accgcctttc acgaggccct ggaggtggcg ttcggattgg tcaaccgggc gaacaagtac
     2281 gccgaggaca ccgccccctg gaagatggtc aaaaccgacg cgccccgaac ccacgccgtg
     2341 ctcacggaga tggcccgggt tttgaaggtg gccgccgtgc cccttccccc ctttatgccc
     2401 accgtcaccg aggaaatgtg gcgacagttg ggcgagcccc gtcccctggg cgccgaggcc
     2461 gcccgggccc tggccggcgg caaaatccaa ttcgaccccg gtcaaaaaat ccgaaaaggg
     2521 aatccgcttt ttccccggaa agaggccccg aaaccttgac cccgccggtc gccatcgaca
     2581 cccacgccca cctggccgac gagaaattcg acgcggaccg ggacgaggtt ttaaaacggg
     2641 cctgggacgc cggattgtcg gtggtcgttg aaatcggcga ggaggaaagc caatggccca
     2701 aggcccgggc cctggccgag gcccatcccg gccgggtgta ctggaccgcc gggtaccacc
     2761 cctattacgc gatcaaagcc gacgacgaaa aattcgacgc ggaccg
//
LOCUS       DAOQLS010000219         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20898_length_2802_cov_5.162723, whole genome shotgun sequence.
ACCESSION   DAOQLS010000219 DAOQLS010000000
VERSION     DAOQLS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20898_length_2802_cov_5.162723"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PK362_05060"
     CDS             <1..509
                     /locus_tag="PK362_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC74238.1"
                     /translation="PMSGPLLVAANHASYIDPPLVGSSLRRPLYFMAKEELFRWPLFG
                     WVIAQVNAFPIRRKEGDVGAFRTARKILSAGGAMIVFPEGRRQRGGVLGRPKGGVGLL
                     AVKSGVPVAPVYLQNTHRAWRFPRLRVVFGKTLTALPGETGDAFAHRVMEAIQQLKES
                     CDGSAVRS"
     gene            487..684
                     /locus_tag="PK362_05065"
     CDS             487..684
                     /locus_tag="PK362_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74239.1"
                     /translation="MDPLSALKPVIQTLVFAVLFLILFVLLLPPLLPLLGQTAGKAVY
                     GVFVLGAVAVALRLRQLARRL"
     gene            complement(681..1766)
                     /locus_tag="PK362_05070"
     CDS             complement(681..1766)
                     /locus_tag="PK362_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74240.1"
                     /translation="MKTAFFPKNRRGIGGARLAVAAALLSLAVVTGVRFFRFSPMDAG
                     ASVPRAALVARGSLSLSNDFHVHWGPIVVDGDLDVSTRPSLTGWPQNNLVSYPQKYSR
                     GALAPWDISPAPPNSDGRRYWAYRDGPPTPALDVEYYRVRAAATRWPLRSAPSTPSLV
                     WSGGEVLSGAGLGVASPTGSGYFSSSLNPGALVFSHFQRRDPGAVVFVEAKDRERYAT
                     GLDAAFLDVEALVLYGARHDVWMSGSSVVFGATIPSRARDEYRDSGAAEVWEMENPPP
                     ARGGPSFAAVGEGRCCYPVPGVNLHGLLYAGGRLLGSGGAPGTVVGLTVLGGDNGRKE
                     WRNVTLYADDGVVNGVRWRSKGPPPGN"
     gene            1883..2608
                     /locus_tag="PK362_05075"
     CDS             1883..2608
                     /locus_tag="PK362_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HNC74241.1"
                     /translation="MKPLSWILRCQRKFADALLAVVNAYLDQYIDGPARSALFDIAAT
                     APALLDVTAAYPVIRAEFDKVFAQRDRLPCYHELDPHVSRISRGDDPVKNWRVYLLNP
                     MGEIPAAARRDCPDTLAVLSKVPEVYQAFFSILDPGKAVPAHRGPFRGYLRYHLGLRV
                     PRERPPVFRIIDHFHTWQEGKDLLFDDCADHEVLNESTEPRGILIVDVLRPLPPTPAR
                     FNRWFVRWVVRTLYGRRLIKNLR"
     gene            complement(2592..>2802)
                     /locus_tag="PK362_05080"
     CDS             complement(2592..>2802)
                     /locus_tag="PK362_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC74242.1"
                     /translation="YRYFPFGAGPRACLGEEFAGATAEAVLQRLFRNFDFETAAGLPR
                     PEPLVTLRPRGGAWAKIRRRVSGDF"
BASE COUNT          483 a          989 c          851 g          479 t
ORIGIN      
        1 ttccaatgtc ggggcctttg ctcgtggccg ccaaccacgc gtcctacatc gatccgcctc
       61 tggtcggatc cagccttcgg cgccccttgt atttcatggc caaggaagag ctgttccggt
      121 ggccgctgtt cggctgggtc atcgcccagg tcaacgcctt tcccattcgt cgaaaggaag
      181 gcgacgtggg ggcctttcga accgcccgta agattctatc cgcgggcggc gccatgatcg
      241 tttttccgga aggtcggcgt cagcgggggg gcgtgctggg ccgccccaag ggcggggtgg
      301 gcctcctggc cgtcaaatcc ggggtgcccg tggcgcccgt ctatttgcaa aacacccacc
      361 gggcctggcg gtttccccga ctgcgggtcg tctttggtaa aaccctgacg gcccttcccg
      421 gcgaaaccgg cgacgccttc gcgcaccgcg tgatggaggc catacaacaa ctgaaggagt
      481 cctgcgatgg atccgctgtc cgctcttaag cccgtcattc aaaccctggt gttcgcggtg
      541 cttttcctga tcctgtttgt tctcctcttg ccgcccctgt tgcccctgct gggccaaacc
      601 gccggcaagg ccgtatacgg cgtcttcgtc ctgggcgccg ttgccgtggc ccttcggctg
      661 cggcaactcg cccgtcgtct ttaatttccc ggaggcggcc ccttcgaccg ccagcgaacc
      721 ccgttgacga ccccgtcgtc ggcgtagagc gtgacgtttc gccactcttt tctcccgttg
      781 tccccgccca ggaccgtgag gccgaccacc gttcccggcg cgccccccga ccccaacaag
      841 cggccgcccg cgtagagcag gccgtgcaga ttcacccccg gcaccgggta gcagcagcgg
      901 ccctccccga cggcggcaaa cgacggcccg ccccgggccg gcggcgggtt ctccatctcc
      961 caaacctccg cggcgccgga atcccgatat tcgtcccggg cccgggaggg gatcgtcgcc
     1021 ccaaagacga cggaacttcc cgacatccaa acgtcgtggc gggccccgta taaaaccaac
     1081 gcctccacgt ccagaaaagc ggcgtccagc cccgtggcgt atcgttcccg gtctttggcc
     1141 tccacgaaca caaccgcgcc cggatcccgc cgctgaaaat gactgaacac gagagcgccg
     1201 ggatttaagc tcgacgaaaa ataaccgctt cccgtcgggg acgccacccc gaggcccgcc
     1261 ccgctcaaca cttcgccccc cgaccacacc aacgacggcg tggacggggc ggaacgaagg
     1321 ggccaccggg tggccgcggc ccggacccgg taatactcga cgtccagggc cggcgtgggc
     1381 gggccgtcgc gataggccca atagcgccgg ccgtcgctgt tcggcggcgc cggggaaatg
     1441 tcccagggcg ccagagcccc ccgggaatat ttttgcgggt aggacacaag gttgttttgg
     1501 ggccagccgg tcaaagacgg acgggtggaa acgtccagat cgccgtcaac cacgatgggc
     1561 ccccaatgca cgtgaaaatc gttggaaagg gaaagggacc cccgggccac cagggccgcc
     1621 cggggaacgg aagccccggc gtccatcggg gaaaaacgga aaaaccggac gcccgttaca
     1681 acggccaggg acaggagggc ggcggcgacc gccaaacggg ccccgccgat gccccggcga
     1741 ttcttcggaa aaaaggcggt tttcacgcgg gggtcggact tagcccgcgc ccgcggccca
     1801 aagcgggccg tccgtcggac gaaccgccgg tttcacggtc gtcccaaaat cggctatgat
     1861 gaggcggaac cattccggcg ccatgaaacc cctctcctgg atcctgcggt gccaacgaaa
     1921 attcgccgac gccctcttgg cggtcgtcaa cgcctacctc gatcaataca tcgacggacc
     1981 cgcgcgttcc gccctgttcg acatcgccgc cacggccccg gcgctccttg acgtcaccgc
     2041 ggcctatccg gtcatccggg cggagttcga caaagtcttc gcccagcggg accgattgcc
     2101 ctgctaccac gaattggacc cccacgtgtc ccgtatttcc cggggggacg accccgtgaa
     2161 aaactggcgg gtctatttgc tgaaccccat gggggagatt cccgccgcgg cccgccggga
     2221 ttgccccgac acgctcgccg tcctttcgaa agtgccggag gtctaccagg cctttttttc
     2281 catcctggat ccgggaaagg cggtgccggc ccaccggggt cccttccggg gttatttgcg
     2341 ctatcacctc ggtcttcggg ttccccggga acgcccccct gtttttcgga ttatcgacca
     2401 ttttcacacc tggcaagagg ggaaagattt gctgttcgac gactgcgcgg accacgaggt
     2461 tctcaacgaa tcgaccgaac cccgggggat tctcattgtc gacgtccttc ggcccctgcc
     2521 gccgacgccc gcccgtttca accgatggtt tgttcgctgg gtggttcgaa ccctttacgg
     2581 ccgccggttg attaaaaatc tccgctgacc cgccgacgaa ttttcgccca cgcgccgccc
     2641 cggggccgaa gggtgaccag gggttccgga cgcggcagac ccgcggccgt ctcaaaatca
     2701 aaattccgaa acaagcgttg gagaacggcc tcggccgtgg ccccggcgaa ttcttccccc
     2761 agacacgccc ggggaccggc cccgaagggg aaatagcgat ag
//
LOCUS       DAOQLS010000220         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20921_length_2800_cov_6.317304, whole genome shotgun sequence.
ACCESSION   DAOQLS010000220 DAOQLS010000000
VERSION     DAOQLS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20921_length_2800_cov_6.317304"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="PK362_05085"
     CDS             complement(<1..1479)
                     /locus_tag="PK362_05085"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNC74243.1"
                     /translation="MNPVDMKARPQDTAKTRPPVRLTENQRKVIQDKYLRTDPSAEVW
                     LERVAANIALAELLHAAEADGWHLWDGVKRKAVDLPTRRGQKSRMNLLHHGLTSSDER
                     DKNFFTFLGNLRKAATEIPEARALAEEWRDKFYAMLARFDFLPNSPTLMNAGRDLQQL
                     SACYVLPVPDTMEGIADALKAQALIHKSGGGTGFSFQRLRPSGDVVKSTKGTASGSIS
                     FMQIFDKMTDVVKQGGTRRGANMGILPYWHPEIEEFIHMKEKPGVMENFNVSVTVDEK
                     FMTAVEKGEDYDLLNPRTMEPTGKKMNAREVFDHMVDSAWKSGDPGIIFIDRINNSAS
                     NPTPHLGQIESTNPCGEQPLLPNEPCNLGSINLANFVEGDLTQGKMNWERLGETVALA
                     VRFLDDVIDVNNYPLPPIEELSKGNRRIGLGVMGWAEALIKMGISYDTDDCLNMAKEV
                     MGFVNDKAMEASEALAEERGAFPNWKGSIFDPDSKHFRGAEHL"
     gene            complement(1635..2078)
                     /gene="nrdR"
                     /locus_tag="PK362_05090"
                     /pseudo
     CDS             complement(1635..2078)
                     /gene="nrdR"
                     /locus_tag="PK362_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
     gene            complement(2510..>2800)
                     /locus_tag="PK362_05095"
     CDS             complement(2510..>2800)
                     /locus_tag="PK362_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74244.1"
                     /translation="RSSPTFSVSVLTGGNLHFTSTGGGTSDVVGFHYVAGNTILEGNS
                     HVHLRGALYTDSGLIGHTAAQGLTITADPNLWADMTPGIPERAVLVKQRPLR"
BASE COUNT          480 a          805 c          967 g          548 t
ORIGIN      
        1 caggtgctcc gcgccgcgga aatgcttgct gtccggatcg aaaatcgacc ctttccagtt
       61 ggggaaggcg ccgcgctctt cggccagggc ctcggaggct tccatggcct tgtcgttgac
      121 gaagcccatg acttccttgg ccatgttgag acagtcgtcg gtgtcgtagg agatgcccat
      181 cttgatcagg gcttcggccc aacccatgac gcccaacccg atccgccggt tgcccttgga
      241 gagctcctcg atgggcggca gggggtagtt gttgacgtcg atcacgtcgt ccaggaagcg
      301 gacggccagg gccacggtct cgcccaggcg ctcccagttc atcttgccct gggtgaggtc
      361 gccctcgacg aaattggcca ggttgatgga ccccaggttg cagggctcgt tggggaggag
      421 gggttgctcg ccgcaggggt tggtgctttc gatttgcccc agatgcgggg tcgggttcga
      481 ggccgaatta ttgatgcggt cgatgaaaat gatgccgggg tcgccggatt tccaagcgct
      541 gtccaccatg tggtcgaaga cttcccgggc gttcattttc ttgcccgtgg gctccatggt
      601 gcgggggttg aggaggtcgt aatcctcgcc cttttccacg gcggtcatga acttttcgtc
      661 cacggtcacg gaaacgttga agttttccat gacgccgggc ttttccttca tgtggatgaa
      721 ctcttcgatc tcggggtgcc aataggggag gatgcccatg ttcgcgccgc ggcgggtgcc
      781 gccctgcttg accacgtcgg tcatcttatc gaagatctgc atgaaactga tggaacccga
      841 ggccgtgcct ttggtggact tcaccacgtc ccccgagggg cgcaggcgct ggaaggaaaa
      901 cccggtgccg ccgccggact tgtggatcaa ggcctgggcc ttcaaggcgt cggcgatgcc
      961 ctccatggtg tcgggcacgg ggaggacgta gcaggcggac aactgctgca gatcccggcc
     1021 ggcgttcatc aaggtcggcg agttgggcaa aaagtcaaag cgggccagca tggcgtaaaa
     1081 cttatcccgc cactcttcgg ccagggcccg ggcttcggga atttcagtgg cggccttgcg
     1141 gagattgccc aggaaagtga aaaagttttt atcgcgttcg tccgaggagg tcagaccgtg
     1201 gtgcaacagg ttcatccggc ttttttggcc ccggcgggtc ggcagatcca ccgctttccg
     1261 cttcacgccg tcccagaggt gccagccgtc cgcctcggcc gcgtgcagga gctccgccag
     1321 ggcgatgttg gcggccacgc gctccagcca gacttcggcc gaggggtcgg tccgcaagta
     1381 tttgtcctgg atgactttcc gctggttttc cgtgagtcgg acgggggggc gggttttggc
     1441 ggtgtcttgg gggcgcgctt tcatgtcaac ggggttcata ggttctcctg ggtcgtgcat
     1501 taaacggatg aaattttgtc cggcgtcgcg gtcaaccggg gacggtccga cgcggggccg
     1561 cgcacaccgg cggcgtcgga accgccgaag cgaaccggcg ggcgcagggt caagcgggcg
     1621 cggcgatcgt ggccccgttt gagcttgcga agctcgctca tgaacgtttc cagatcgccg
     1681 aactggcggt acacggaggc gaagcgcacg tacgccacgg ggtcgagatc gatgagcttc
     1741 ttcaagatcc gctcgccgat caccgggctg ggcacttcca acacgaagtc ttgcaggctg
     1801 tattccactt cggccaccag cttttcgatg gccgccgggg aaatcgggcg tttctgacag
     1861 gcgcgcacga tgccttcgcg catcttttcg cggcggaaag gttcccgccg gttgtcgcgt
     1921 ttgaccacca tcaggggggt ggattcgacc cgctcgtagg tggtgaagcg tttgccgcaa
     1981 tggacgcagg atcggcgccg ccggatcacg gaggcggact ccagcggacg gctgtcgatc
     2041 acgcggtctt caggatggtc gcaagcgggg catctcatac aacgggtggg gtcactcctc
     2101 cctcgattat acaacatctt gattccggag gccctggcga ggggccccat atcttgtggc
     2161 cggggcagat attactccac cacctgtggt gtgtcaagga cggcaaacat cgacggtaaa
     2221 aagaggggtc ttcggcggaa aaatcgcgga tgtaaatttt tttaaacgct gattttccgt
     2281 cggggaaggg gagccgcggc gggggctccg gggaagccaa aaacgcgaaa agccgggtcg
     2341 cccggcccgt tgtcgacgga aaacttcgcc cgcgggaaag gccatcgacg gaaagggaga
     2401 acggactttc ccccgcggga atttcgggcc ggtggactcc acaagggggc cggggggcgc
     2461 gcggcgcccg ccgcccggag gaggggcgat cggcgcggga aaaaaccggt taccgtaggg
     2521 ggcgctgttt gaccaacacg gcccgctcgg ggatgccggg ggtcatgtcg gcccacaaat
     2581 tgggatcggc cgtgatggtc aatccctggg cggcggtgtg cccgatgagc ccgctgtccg
     2641 tgtagagggc cccgcgcaga tggacgtggg aattcccctc caaaatcgtg ttcccggcca
     2701 cgtagtggaa ccccacgacg tcggaggtgc cgccgccggt gctcgtgaaa tggaggttgc
     2761 ctccggtgag gaccgacacg ctgaacgtgg gcgaggagcg
//
LOCUS       DAOQLS010000221         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20953_length_2798_cov_2.725118, whole genome shotgun sequence.
ACCESSION   DAOQLS010000221 DAOQLS010000000
VERSION     DAOQLS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20953_length_2798_cov_2.725118"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PK362_05100"
     CDS             <1..228
                     /locus_tag="PK362_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74245.1"
                     /translation="PAGVRLLVEDAETGRPVSVPAAAAGGAIDRHNGQRRDRLARDLR
                     RSGTDAVFAETGPFEAKAFLKFFHERAKRQH"
     gene            203..1132
                     /locus_tag="PK362_05105"
     CDS             203..1132
                     /locus_tag="PK362_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNC74246.1"
                     /translation="MNAPNANIKRRLTILLLLGAAPLRAGEARLRLETTVDPSTATVG
                     DRLRLTVRAVAEGDVRISSPSVGGALGIFEVAGWEVEEAPGPGASPGRVFRYTLVPFD
                     VGVATIPALSVPYRFGGGPVQYQPTPEIFVQVRSVLPPDAGDIRGLKGRLPRVPNEWF
                     WGLLAAGTVLAFLVYFLRTRRRPGTDLPPGPPPRPAHLVALEALDRLEKDLAGPAKPF
                     YSRLTDIWRAYLEARFGLPALDRTSGEIAQSLRNLPVSSEQKRRLREWLDVSDLAKFA
                     KTEPGNEARLAHLDLVRSFVDSTVPAPTDGRVS"
     gene            1129..2124
                     /locus_tag="PK362_05110"
     CDS             1129..2124
                     /locus_tag="PK362_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC74247.1"
                     /translation="MIFAHPNVLWGLLLLPLLALYEIRWGAGAKARLRLSSLDLLPPG
                     LGTAVDGPSRILAGLRLGALAFAFVALARPQRGQERQEVTSPATDIVLCLDVSDSMRS
                     LDFKPKNRLDAALEVIKEFVKHRSADRIGLVLFANGAFTQCPLTLDHGALMGFLDNVG
                     IGVVDPQRTAVGTAIATAVGRLKKSEAKSKVIVLLTDGQSNAGEVDPPTAARAAAAFG
                     IKIYTVGAGAPGGGLIEVDDPFFGKRLVRTPENELDEATLRRVAEDTHGVYFRATDLA
                     SLKKIYGEIDRMEKTDVKTQTFAEYRDVYPVWLWGAFLLLAAEGILSQTLWRRYP"
     gene            2124..>2798
                     /locus_tag="PK362_05115"
     CDS             2124..>2798
                     /locus_tag="PK362_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC74248.1"
                     /translation="MWTAPALLALLVVPAAVGLLTVAAFRWKRRTTARLGDPAVVARL
                     VDPRTGRYQRGKALLRFLAGIFLALALAGPRWGSLFQEIHRRGIDVVIAVDVSASMLA
                     EDVKPNRLTQAKRELTLLIDGLEGDRVGLVAFAGAAFLQCPLTLDYGAARTLLDLIGP
                     DLIPRPGTDLAAALRQALDAFPRGLHQYKAIVLLTDGEDHSGALDAAVARAAEEGVRI
                     FPVGFGS"
BASE COUNT          430 a          925 c          923 g          520 t
ORIGIN      
        1 cccgccggcg tccgcctgtt ggtggaggac gccgaaacgg gtcgccccgt cagcgttccg
       61 gcggccgccg ccggcggggc catcgaccgc cacaacggcc agcgccggga ccggttggcg
      121 cgcgacctcc gccggagcgg caccgacgcc gttttcgccg aaacgggccc cttcgaggcc
      181 aaagcgtttc tcaaattttt ccatgaacgc gccaaacgcc aacattaaac gccgcctgac
      241 gatcctcctt ctcctgggcg ccgcgcccct gcgggccggg gaggcccgac tgcgcctcga
      301 aacgaccgtg gatccctcca cggcgacggt gggcgaccgc ctccgcttga ccgttcgcgc
      361 cgtggcggag ggcgacgttc gaatctcctc gccgtcggtg gggggggcgt tgggaatttt
      421 cgaagtcgcc gggtgggaag tcgaagaagc cccggggccg ggcgcttcgc cgggacgggt
      481 ctttcgctac accctggtcc ccttcgacgt cggcgtggcc acgatcccgg ccctgtccgt
      541 gccttaccga ttcggcggcg gccccgttca ataccagccg acgcccgaaa tcttcgtcca
      601 ggttcggtcg gtcctgccgc cggacgccgg ggacatccgc ggcttgaaag ggcgattgcc
      661 gcgggtgccg aacgaatggt tttggggact cttggccgcc gggacggtgt tggcttttct
      721 cgtttatttt ctccgaacac gccgacggcc cgggacggat cttccgcccg gtccgccccc
      781 ccggccggcc cacctcgtgg cgctggaggc cctggatcgg ttggaaaaag acctggccgg
      841 cccggcgaaa cctttttaca gccgtttgac ggacatctgg cgggcctacc tggaggcccg
      901 gttcggcctg cccgccctgg accggacgtc gggggaaatc gcccaatccc tccggaacct
      961 tcccgtgtcc tcggagcaga aacgccgcct tcgggaatgg ctcgacgtga gcgacctggc
     1021 caaattcgcc aagacggagc cggggaacga agcccgcttg gcccatttgg acctggttcg
     1081 atcgttcgtt gattccaccg ttcccgcgcc cacggacggg agggtttcgt gattttcgcc
     1141 catcccaacg tcctgtgggg gcttcttctc ctgccgcttc tggcccttta cgaaatccgg
     1201 tggggggcgg gggccaaagc ccgccttcgc ctgtcgtccc tggaccttct tcccccgggc
     1261 ctgggaacgg cggtcgacgg accgtcccgg attctggccg gccttcggtt gggggccttg
     1321 gcttttgcgt tcgtggcgct ggcccgtccc cagcggggac aggaacgcca ggaagtcacg
     1381 agccccgcca ccgacatcgt cctttgtctg gacgtttcgg acagcatgcg aagtctggat
     1441 tttaaaccca aaaaccgctt agacgccgcg ttggaagtga tcaaagagtt tgtgaagcat
     1501 cggtccgcgg accgaatcgg tttggtcctc ttcgccaacg gggccttcac ccaatgcccc
     1561 ctgaccctgg accacggggc gctgatgggt ttcctggaca acgtgggcat cggggtcgtg
     1621 gacccccagc ggacggccgt gggaacggcc atcgccaccg cggtcgggcg actaaaaaaa
     1681 agcgaggcca aaagcaaggt gatcgtccta ctgaccgacg ggcaaagcaa cgccggcgag
     1741 gtcgacccgc ccaccgccgc ccgggcggcg gcggccttcg ggatcaaaat ttacaccgtg
     1801 ggggccggcg ccccgggcgg gggccttatt gaggtggacg accccttttt cggaaagcgc
     1861 ctcgtgcgaa ccccggaaaa cgaattggac gaagccaccc tccgtcgcgt ggcggaggac
     1921 acccacggcg tctatttccg cgccacggac ttggcgagcc tgaagaaaat ttacggggaa
     1981 atcgaccgga tggaaaaaac cgatgttaaa acccaaacct tcgccgaata ccgggacgtc
     2041 tatcccgtct ggttgtgggg ggcgtttctg ttgttggccg ccgaaggaat cctgtcccaa
     2101 accctgtgga ggcgctaccc gtgatgtgga ccgcgccggc tctgttggcc ctcctggtcg
     2161 ttcccgccgc ggtgggcctg ctgacggtgg cggccttccg ttggaaacgg cggacgaccg
     2221 cccggttggg cgatcccgcg gtggtggccc ggttggtgga cccccggacc ggtcgttatc
     2281 aacggggcaa agcccttctt cgctttctgg ctggaatttt tctcgcgctg gctttggcgg
     2341 gtccccggtg gggttctttg tttcaggaaa tccaccgccg gggaattgac gtggtgatcg
     2401 ccgtggacgt ttcggccagc atgttggccg aggacgtcaa acccaaccgc ctgacccagg
     2461 ccaaacgaga attgaccctt ttgatcgacg gtctagaagg cgaccgggtg ggtttggtgg
     2521 cctttgccgg agcggctttt ttgcaatgcc ccttgaccct ggactacggg gcggcccgga
     2581 ccctgttgga tttgatcggc ccggatttga tcccccggcc gggcaccgat ttggcggcgg
     2641 ccctgcgaca ggccctggac gcctttcccc ggggcctcca ccaatacaaa gcgatcgtgc
     2701 tcctcaccga cggcgaggac cattccgggg ccctggacgc cgccgtggcg cgggcggcgg
     2761 aggagggcgt ccgaattttt cccgtcgggt tcggcagt
//
LOCUS       DAOQLS010000222         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_20990_length_2795_cov_4.372263, whole genome shotgun sequence.
ACCESSION   DAOQLS010000222 DAOQLS010000000
VERSION     DAOQLS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20990_length_2795_cov_4.372263"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PK362_05120"
     CDS             <1..826
                     /locus_tag="PK362_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNC74249.1"
                     /translation="SIEVSPLLARNTDATLAEARRLWKWVDRPNVMIKIPATREGIPA
                     IQRCLTDGININVTLIFSLERYDEVAQAYRAALETRAQQGLGVGVASVASFFISRIDT
                     SVDKLVQEKIQTSPSAKEKDALLELLGKVAIANAKAAYRLFQKRFSDEKFAALRTLGA
                     RPQRPLWASTGTKNPHYSDVLYVDQLIGASTVNTLPPATLAAFLDHGQVADTLESDAD
                     EALKTLERLKAAKIDLRRGTETLEAEGVRLFAESYEKLIASLRAKKQALELAAAKG"
     gene            831..2555
                     /locus_tag="PK362_05125"
     CDS             831..2555
                     /locus_tag="PK362_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNC74250.1"
                     /translation="MKNKTVAAGLPAQLPGIDLPEALGELKEENVPQRLWAKDPSLWK
                     SDPQIQNAIRHRLGWLDLPATMGSAVAELTAFAAEIRRAGVKDVVLLGMGGSSLCPEV
                     LRQIIGARVGFPRLSVLDTTDPGAILAVTKAIQLRKTLFIVSSKSGGTIEVNSLFKYF
                     WAKVEALQKAKAGASFVAVTDPGTMLERLAKEKKFRRVFLAPPDVGGRYSALTYFGLL
                     PAALLGIDLKRFLDRAARVANASGPGRDPSENPAVWLGAVLGAAAEAGRDKVTLWTSP
                     ELRAFGIWAEQLIAESTGKEGAGLVPAESEPLAPGRVDGRDRVFVFLGLKGSKGNAPA
                     LKALAALEKAGHPVVRLELADRYDLAGEFFRWEMATAVAGQLMGINPFDEPNVSESKA
                     NTTRILAQFEAARALPEETPAVVEKGVALWSTNKVRGDSVNGVLSDFLSATRPGDYVA
                     VLAYVTPDKTRTGALQALRRRVGELTGRATTLGFGPRFLHSTGQLHKGGAGNGVFLQI
                     TAADATDAVVPGAGYGFATLKMAQALGDFQSLREHGRRAVRIHLSKNATRDLAAIVGK
                     LKAAVPAA"
     gene            2566..>2795
                     /locus_tag="PK362_05130"
     CDS             2566..>2795
                     /locus_tag="PK362_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC74251.1"
                     /translation="MDIGFVGLGRMGANMVERLLRGGHRVVAYNRSPEKTRDVMAKGA
                     EGVFALADFAKTLKPPRAVWVMVPAGDATETTL"
BASE COUNT          525 a          868 c          885 g          517 t
ORIGIN      
        1 gtccatcgaa gtgtccccgc ttctcgcccg caacaccgac gccaccttgg cggaggcgcg
       61 tcgtttatgg aaatgggtcg atcgcccgaa cgtcatgatc aagattccgg ccacccggga
      121 gggaattccc gccatccaac ggtgtctgac cgacgggatc aacatcaacg tcacgctcat
      181 cttttctttg gaacgttacg acgaagtcgc tcaggcctac cgcgcggccc tggaaacgag
      241 ggcccaacag gggttgggcg tcggcgtggc cagcgtggcg agctttttca tcagccggat
      301 cgacacctcc gtcgataaat tggtccagga aaaaattcaa acctctccca gcgcaaaaga
      361 gaaagacgcc ctgttggagt tgctcgggaa agtcgccatc gccaacgcca aagcggccta
      421 tcgattgttc cagaaacgat tttccgatga aaagttcgcc gccctgcgca ccttgggcgc
      481 gcgtccccag cgccccctct gggccagcac ggggaccaag aacccccatt actccgacgt
      541 tctctacgtc gatcagttga tcggcgcttc gaccgtcaac actttgcccc ccgcaaccct
      601 ggccgcgttc ctggaccacg ggcaggtggc cgatacgttg gagtccgacg cggacgaagc
      661 gctgaaaacg ctggagcggc tcaaagccgc caaaatcgat ttgcggcggg ggaccgaaac
      721 cctggaagcc gaaggcgttc gtttgttcgc cgaatcctac gagaagttga tcgccagcct
      781 ccgggccaaa aaacaggcgt tggaattggc cgccgccaaa ggatgattcc gtgaaaaaca
      841 aaaccgttgc cgcgggcctg cccgctcagc ttccgggcat tgatttgccc gaggccttgg
      901 gggaattgaa agaggagaac gtcccgcagc gcctctgggc caaagacccc tccctttgga
      961 agtccgaccc gcaaatccaa aacgcgatcc gccaccggtt ggggtggctg gatctgcccg
     1021 ccaccatggg cagcgccgtg gcggagttga ccgccttcgc cgccgaaatt cgacgggcgg
     1081 gcgtgaagga cgtcgtcttg ctgggcatgg gcggttccag cctgtgtccg gaagtgttgc
     1141 gccaaatcat cggtgcccgg gtcgggttcc cccggttgtc ggtgttggac acgacggacc
     1201 ccggcgccat tctcgccgtg acgaaggcga ttcaactccg aaaaacgctt ttcatcgtct
     1261 ccagcaagtc gggcgggacg attgaagtca attccctgtt caaatatttt tgggccaaag
     1321 tcgaggccct tcaaaaagcc aaagccggag cgtcctttgt cgcggtcacc gaccccggaa
     1381 cgatgctgga gcgcttggcc aaggaaaaga aatttcgtcg cgtgtttttg gctcccccgg
     1441 acgtgggcgg tcgatattcc gcgctgacgt atttcggttt actgcccgcc gcgttgttgg
     1501 gcattgattt gaaacgattt ttggatcggg cggcgcgggt cgccaacgcc tcggggcccg
     1561 gtcgggatcc gtcggagaac ccggccgtgt ggctgggcgc ggtgttgggc gcggcggccg
     1621 aagccgggcg ggacaaagtc accttatgga cctcgccgga attgcgggcc ttcgggattt
     1681 gggcggaaca gctcatcgcg gagagcacgg gcaaagaggg ggccggtctc gttcccgcgg
     1741 agagcgagcc tttggccccg ggccgcgtgg acgggcgcga tcgcgtcttc gtttttttgg
     1801 gtctcaaggg atccaaaggc aacgccccgg ccttgaaagc cctggccgcg ctggaaaagg
     1861 cgggccaccc ggtcgttcgt ttggaacttg ccgatcgcta cgatttggcg ggggaatttt
     1921 tccggtggga aatggccacc gcggtcgccg gtcaattgat gggcatcaac cccttcgacg
     1981 aaccgaacgt atccgaaagc aaagccaaca ccacgcggat tttggcgcag ttcgaggcgg
     2041 cccgggccct gcccgaggaa acgcccgccg ttgtggagaa gggcgtcgcg ctttggtcaa
     2101 cgaacaaggt tcggggggat tcggtgaacg gggtcctttc ggatttcctg tccgccaccc
     2161 gtcccgggga ttacgtcgcc gttctggcct acgtgactcc cgacaaaacg cgaacggggg
     2221 cccttcaggc cttgcgtcgg cgggtggggg aattgacggg ccgggccacc acgctggggt
     2281 tcggtccccg gttcctccac tccacgggcc agctccacaa aggcggggcg ggcaacggcg
     2341 ttttcctgca aattaccgcc gcggacgcga ccgacgccgt cgtcccgggc gccggttacg
     2401 gttttgccac gttgaaaatg gcccaagcct tgggcgattt ccagtcgttg cgggagcacg
     2461 gccgtcgggc cgttcgaatt catctttcaa aaaacgcgac gcgcgatctg gccgccatcg
     2521 tcggaaaact caaagccgcc gttcccgccg cctaggggat caatcatgga catcggtttc
     2581 gtcggcctcg gacgcatggg ggccaacatg gtggagcgcc ttttgcgggg cggccaccgg
     2641 gttgtcgcgt acaaccgatc gccggaaaaa acccgcgacg tcatggccaa gggcgccgaa
     2701 ggcgttttcg ccctggccga tttcgccaaa accctcaaac cgccccgggc cgtctgggtc
     2761 atggtgccgg ccggggacgc caccgaaacc accct
//
LOCUS       DAOQLS010000223         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21017_length_2793_cov_3.951059, whole genome shotgun sequence.
ACCESSION   DAOQLS010000223 DAOQLS010000000
VERSION     DAOQLS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21017_length_2793_cov_3.951059"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK362_05135"
     CDS             complement(<1..777)
                     /locus_tag="PK362_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74252.1"
                     /translation="MTRALAAFLLIFGAAFPAGAAPAAKPATGAPAAPEAAPPPTAFS
                     PRETMDALIDEGLRAIYNLDYAGSKAAFATLKRDFPDHPIGLYGLTTALWWELTNDYD
                     EDNPVLEKEFLATADETIAFARAQLKKGDPTGEIRLCLGGALGLKGRWDAIQGHWLAA
                     YKNGKKAFDVQDEAIKSNPELYDAYLGVGIFHYYVAVLPAAVKIFARMIFGGSKAQGL
                     REINLAKDKGRFSRTAALLFLVNLHVNNEKDPATALALLRE"
     gene            complement(774..1952)
                     /locus_tag="PK362_05140"
     CDS             complement(774..1952)
                     /locus_tag="PK362_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HNC74253.1"
                     /translation="MKWASAISQQVRLESALKECAEGVRTQLGEAPVDFAALFVSDNF
                     AGRYEEAHALLADRLAVKTLIGASGGGVIGDGQEVEHRPAVSLVAARLPKVRVRPFSV
                     QDEDLPDLDASPRAWESIVGVRAGEDPQFLVLADPFSIRSDNFLLGLDYAFPKSAKIG
                     GLASGASEPGRNALYLNNLCLRSGLVGVSFTGDILVDTVVAQGCRPIGKPFHITECND
                     NVLASLDGKPPLQALQDLFQTLPERDRQLLRQSLFLGIAMNSTIGEPRAGDFLVRNII
                     GVDNERGLLAIGAPLRVGQVVQFHLRDAQSSADDLKQVFDRVGPVRAEARGAFLFSCV
                     GRGQSLYGRPHHDSRFFQQQVGPVPLGGLFCNGEIGPVGGTTYLHGYTSCFGLFRPKS
                     "
     gene            2072..2383
                     /locus_tag="PK362_05145"
     CDS             2072..2383
                     /locus_tag="PK362_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNC74254.1"
                     /translation="MVLLEFSLFPIGKGQSVGRYVARSLDLIDKSGVPYELHSMGTIL
                     EGEWDEVFAVVRKCYERMRKDCPRLALTLKVDYRRGARGRLKSKVASVERRVGRKLGR
                     P"
     gene            2380..>2793
                     /locus_tag="PK362_05150"
     CDS             2380..>2793
                     /locus_tag="PK362_05150"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNC74255.1"
                     /translation="MTAYILDGYNVIRSVDWLATGPLRDQRDRLLRFVEDRRPQGNAE
                     VIVIFDGRADVSGPRWGGPTRVIYSDGEADPHLKRLVDERQNPRDVVVVTDDRAIERW
                     VKAAGAKVMRCRDFLAAGAGAPRARKAGRQLAPQDV"
BASE COUNT          478 a          864 c          992 g          459 t
ORIGIN      
        1 ctcccggaga agggccaggg cggtggccgg gtctttctcg ttgttgacgt ggaggttcac
       61 caggaacagc agggccgccg tccgggaaaa gcgtcccttg tccttggcga ggttgatttc
      121 ccgaaggccc tgggctttgg atccgccgaa aatcatccgg gcgaaaattt tcaccgccgc
      181 gggcaggacc gccacgtagt aatggaaaat gccgacgccg aggtacgcgt cgtaaagttc
      241 ggggttcgat ttgatggcct cgtcctggac gtcaaaggcc ttcttgccgt ttttgtaggc
      301 cgcgagccag tggccttgaa tggcgtccca ccgccctttg aggcccaggg cgccgcccag
      361 gcacaggcgg atttccccgg tcgggtcgcc ctttttcaat tgcgcccggg cgaaggcgat
      421 ggtttcgtcg gccgtggcca gaaattcctt ttcgagaacg gggttgtcct cgtcgtagtc
      481 gttggtcagc tcccaccaga gagccgtcgt gaggccgtag aggccgatgg ggtggtccgg
      541 gaaatcccgt ttgagggtgg cgaaagcggc tttggagccg gcgtagtcca ggttataaat
      601 ggcgcgcagg ccctcgtcga tcagggcgtc catggtttcc cgggggctga aagccgtagg
      661 cggcggggcg gcctcggggg cggccggagc cccggtcgcc ggtttcgccg cgggggccgc
      721 gccggcggga aaggccgcgc cgaaaatcag cagaaaggcc gccagcgcgc gggtcatgat
      781 ttgggacgga agagcccgaa gcagctggtg tacccgtgaa ggtaggtggt tcccccgacg
      841 ggaccgattt cgccgttgca gaaaagaccc cccagcggga cggggcccac ttgctgttgg
      901 aaaaaccggg agtcgtgatg ggggcgtccg tagagcgatt ggccccggcc cacgcaggag
      961 aacagaaagg ccccgcgggc ttccgcccgc acgggaccca cccggtcaaa gacctgtttc
     1021 aaatcgtcgg cggaggattg ggcgtcccgc aaatggaatt gaaccacctg gcccacccgc
     1081 aggggcgcgc cgatggccag caggccccgt tcgttgtcca cgccgatgat gttccgcacc
     1141 aggaagtcgc cggcgcgggg ttcgccgatg gtcgagttca tggcgatgcc caaaaagagc
     1201 gattggcgga ggagctggcg gtcccgctcg gggagggttt ggaacaaatc ctgcagggcc
     1261 tggaggggcg gtttgccgtc cagggaggcc aaaacgttgt cgttgcattc ggtgatgtga
     1321 aagggtttgc cgatggggcg gcacccctgg gcgaccaccg tgtccacgag gatgtcgccg
     1381 gtgaaggaaa cgcccaccag gcccgaccga aggcagaggt tgtttaggta aagggcgttc
     1441 cgcccgggtt ccgaggcgcc ggaggccagc ccgccgatct tggcggattt ggggaaggcg
     1501 taatccaacc ccaggaggaa attgtcggag cggatggaaa aggggtcggc cagcaccagg
     1561 aactgggggt cctcgcccgc ccggacgccg acgatggatt cccaggcgcg gggggaagcg
     1621 tcgaggtccg gcaagtcctc gtcttgaacc gaaaaaggcc ggacgcgcac tttgggcagg
     1681 cgcgcggcaa cgaggctcac ggccgggcgg tgctccacct cctgcccgtc gccgatcaca
     1741 ccgcccccgc tggccccgat cagggttttg accgccagtc ggtcggccag cagggcgtgg
     1801 gcttcctcgt aacgcccggc gaaattgtcg gagacgaaca gggcggcgaa atccaccggg
     1861 gcttccccca attgcgtgcg gacgccctcg gcgcactcct tgagcgctga ttccaaccgc
     1921 acctgctggg agatggccga agcccacttc attgggcgtg atgataacac cttgggaccc
     1981 cgacgggaac ccccggcccg cgggacgctc ggcgggggaa tccccgcccg gcgggtttac
     2041 gtcggattgt ggatggcgta tcatgttccc catggtcctc ctggaattca gtttattccc
     2101 catcggcaaa ggtcaaagcg tcgggcgcta cgtcgcccgg tccctggacc tcatcgacaa
     2161 aagcggcgtg ccctacgagc tccattccat gggcaccatc ctggaagggg aatgggacga
     2221 ggtcttcgcc gtggtccgaa agtgttacga gcgcatgcgc aaggactgcc cccgtctggc
     2281 cctcacgctc aaggtggatt accgccgggg cgcccggggc cgcctgaaat ccaaggtggc
     2341 cagcgtcgaa cggcgggtcg gccggaagct cgggcgcccg tgaccgccta tattctggac
     2401 ggctacaacg tcatccgcag cgtcgactgg ctggccaccg gccccctgcg ggaccagcgg
     2461 gaccgcctgc tccggtttgt ggaggaccga cggccccagg ggaacgccga ggtgatcgtg
     2521 attttcgacg ggcgggccga cgtcagcggc ccccgctggg gcgggcccac ccgggtgatc
     2581 tattccgacg gcgaggccga tccccattta aagcgcttgg tcgacgagcg tcaaaatccc
     2641 cgggacgtgg tggtggtcac cgacgaccgg gccatcgagc gctgggtcaa agccgccggg
     2701 gccaaggtga tgcgctgccg ggattttctg gccgccggcg ccggggcgcc ccgggcccgc
     2761 aaggcggggc gccaactggc cccccaggat gtg
//
LOCUS       DAOQLS010000224         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21337_length_2773_cov_4.196836, whole genome shotgun sequence.
ACCESSION   DAOQLS010000224 DAOQLS010000000
VERSION     DAOQLS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21337_length_2773_cov_4.196836"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK362_05155"
     CDS             complement(<1..316)
                     /locus_tag="PK362_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNC74256.1"
                     /translation="MADAARRIELKSESDLARMRRAGALAAQALAEAGRAVAPGVTTK
                     DLDRIAEKVIRGGGGIPTFVGYRGYPATICASINEEVVHGIPSAQRVLKEGDIVGIDV
                     GAT"
     gene            complement(320..976)
                     /locus_tag="PK362_05160"
     CDS             complement(320..976)
                     /locus_tag="PK362_05160"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNC74257.1"
                     /translation="MNIVLLGAPGSGKGTQAKPLSVRYGVPHISTGDIFRQEMAKSSD
                     LGLKVQQYVSSGRLVPDELVLEIVTARLAAADCAPGFILDGFPRTVSQAEGLDAFLNG
                     AHRTLDKVLYMDLSEKEVVQRLSSRRSCPKCGKVYNLLTQAPRQADVCDVDGEKIIQR
                     EDDRPDTVEKRLVVYNNLTEPLISYYRGLGVLEKISAAQSVADVQRAIFNVLDALPKA
                     "
     gene            complement(999..2339)
                     /gene="secY"
                     /locus_tag="PK362_05165"
     CDS             complement(999..2339)
                     /gene="secY"
                     /locus_tag="PK362_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNC74258.1"
                     /translation="MQTFADVFKIPDLRKRVLFTLGILAIYRVGVAIPIPGIEAAALQ
                     QLFKSQSNAFLGFLDMFSGGALGRMSILAMGVVPYINSSIIMSLLQGAHVIPYLDRLA
                     KEGETGRKKLNQITRYFTLFLALIQSFGLTTLITKIQLPGDIPVVRDPGALFMFTTVV
                     TLATGTIFMMWLGEQITERGIGNGTSLMIFTGIVDRLPGAVRNMVTEVFQLQQRSLVG
                     TVAIGAFIIVVMGLVVWVETAQRKIPVQHARRMVGRRMFGGASTFLPLKVDQSGVIAV
                     IFAISLLSVPVTVASFNHDGAWAKKVLEFWNHRAWWYETLYAGLIIFFCYFYNSVSIN
                     PVDMADNLKKAGAFIPGIRPGEPTAAHISTVLARVTLGGAVFVAALAILPDMLHRLFN
                     TPFNFGGTSILIVVGVALDTVGQLESHLIMRHYEGFSKAGRVKGRWFNVGAASQ"
     gene            complement(2352..>2773)
                     /gene="rplO"
                     /locus_tag="PK362_05170"
     CDS             complement(2352..>2773)
                     /gene="rplO"
                     /locus_tag="PK362_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNC74259.1"
                     /translation="RRKIVGRGEGSGHGGKGSTRGFKGQTARSGDGSLGAGFEGGQIP
                     LIRRIPKRGFNNTAFRKSYELVNVGDLMTFFAKAGEVTPESLRAAGLVRSRGPIKILG
                     DGDLSRAYHVKANAFSAGAKKKIESAGGKAELISASK"
BASE COUNT          544 a          872 c          874 g          483 t
ORIGIN      
        1 gagtggcgcc cacgtcgatc cccacgatgt cgccctcttt caaaacgcgc tgggcgctcg
       61 ggatgccgtg gaccacttcc tcgttgatcg acgcgcagat ggtggcggga tatccccggt
      121 aacccacaaa ggtcgggatc ccgcccccgc cgcgaatcac tttttccgcg atccgatcca
      181 aatctttcgt cgtgaccccg ggggccacgg cgcgccccgc ttccgccagg gcttgagccg
      241 ccaaggcgcc cgcccggcgc atgcgggcaa ggtcggactc ggattttaat tcaatccgtc
      301 gcgcggcgtc cgccaccgct cacgccttgg gcagggcgtc caaaacgttg aaaatggcgc
      361 gctgaacgtc ggccaccgac tgggcggccg aaatcttttc caacaccccg agaccccggt
      421 agtaggaaat caggggttcg gtcaaattgt tgtacaccac caaccgcttc tcgacggtgt
      481 cgggacgatc gtcttcccgc tgaatgatct tttcgccgtc cacgtcgcaa acatcggcct
      541 gccggggcgc ctgggtgagc agattgtaga ccttcccgca tttcgggcag gaccggcggc
      601 tggagaggcg ctggaccact tccttttccg acaaatccat gtaaagaacc ttgtccaggg
      661 ttcggtgggc gccgttcaag aaggcgtcca agccctcggc ctgggagacc gtccggggaa
      721 acccgtccag gatgaagccc ggcgcgcagt cggccgcggc cagacgggcc gtgacgatct
      781 cgagcaccag ctcgtcgggc accaggcggc cggagctcac gtactgctgg accttgaggc
      841 ccaaatccga ggacttcgcc atttcctggc ggaagatgtc ccccgtggaa atgtgcggca
      901 cgccgtaccg gacggacaac ggtttggcct gggtgccttt tcccgaaccg ggggcgccca
      961 acaggacgat gttcatcgtt tccctccagg ggaaaaaatc actgggaagc ggcgcccacg
     1021 ttgaaccagc gccccttgac ccgcccggcc ttggaaaacc cctcgtagtg ccgcatgatc
     1081 aaatgggatt ccagttgacc cacggtgtcc agcgccacgc ccacgacgat caaaatcgac
     1141 gttccgccga agttgaaggg cgtgttaaac aatcggtgca gcatgtccgg caggatcgcc
     1201 agggcggcca cgaacaccgc gccgccgagc gtcacccggg ccaggaccgt gctgatgtgg
     1261 gccgccgtcg gctccccggg ccggatgccc gggatgaagg cgccggcctt cttcaaattg
     1321 tcggccatgt cgacggggtt gatggagacc gagttataaa aatagcaaaa gaagatgatc
     1381 aaacccgcgt agagcgtctc gtaccaccag gcccggtggt tccaaaattc caaaaccttt
     1441 ttggcccagg cgccgtcgtg gttgaaggac gccacggtca ccggaacgct caaaagcgaa
     1501 atggcgaaga tcacggcgat cacgccggat tggtcgacct tgagcggcaa aaaggtgctg
     1561 gccccgccga acatccggcg gcccaccatg cggcgggcgt gctggacggg gatcttgcgc
     1621 tgcgcggttt ccacccagac caccaggccc atcacgacga tgatgaaggc gccgatggcc
     1681 accgtgccca ccagggaccg ttgttgaagt tgaaaaacct ccgtcaccat gttgcggacg
     1741 gcgccgggca ggcggtcgac gatgccggtg aaaatcatga gggaggtgcc gttgccgatg
     1801 ccgcgctcgg tgatctgctc gcccagccac atcatgaaga tcgtgccggt ggccagggtg
     1861 acgaccgtcg tgaacatgaa gagagcgccc gggtcccgca ccacgggaat gtcgcccggc
     1921 agctggatct tggtgatgag cgtcgtcaac ccgaaggact ggatcagcgc cagaaagagg
     1981 gtgaagtacc gcgtgatctg gttgagcttt ttccgccccg tctcgccttc cttggccaac
     2041 cggtccaagt agggaatgac gtgggccccc tgcaacaagc tcatgatgat ggaggagttg
     2101 atgtacggca cgacccccat ggccagaatc gacatgcggc ccagggcgcc gcccgaaaac
     2161 atgtcgagga aacccaggaa cgcgttggat tgggatttaa agagctgttg aagggccgcc
     2221 gcttcgatcc ccggaatggg gatggcgacg ccgacgcggt agatcgccaa aatccccagg
     2281 gtgaagagga cccgcttgcg caggtcggga attttgaata cgtccgcgaa ggtttgcatg
     2341 gatcggccgg gttatttcga cgcggaaatc agttccgctt tgccgccggc cgattcgatc
     2401 tttttcttgg cgcccgccga gaaggcgttg gccttcacgt gatacgcccg ggacagatcg
     2461 ccgtccccca aaatcttgat cggcccgcgg gagcggacca gaccggccgc ccgcaacgac
     2521 tcgggcgtga cttcgccggc cttggcgaaa aaagtcatca ggtcgccgac gttgaccagt
     2581 tcgtagcttt tgcggaaagc ggtgttgttg aaaccgcgct tgggaatgcg ccgaataagc
     2641 gggatctggc cgccttcgaa accggcgccc agggaaccgt cgccggaacg agccgtctgg
     2701 cccttgaaac cgcgggtgga gcctttgccg ccgtgcccgg agccttcgcc ccggcccacg
     2761 attttgcggc ggt
//
LOCUS       DAOQLS010000225         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21403_length_2768_cov_3.566532, whole genome shotgun sequence.
ACCESSION   DAOQLS010000225 DAOQLS010000000
VERSION     DAOQLS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21403_length_2768_cov_3.566532"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PK362_05175"
     CDS             <1..1393
                     /locus_tag="PK362_05175"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC74260.1"
                     /translation="EIIADSIETVAGAERFDGLVAIGGCDKNMPGCLMAMGRLNIPAV
                     FVYGGSIRPGSFQGKDVDIVSIFEAVGQFNNNLIDEKTLKGVECSACPGPGSCGGMYT
                     ANTMASAIECLGMSLPGSSSYPAETAEKVAECAEAGKAVVQALRLGLRPRDIVKKKNF
                     ENAIALVMVLGGSTNAVLHLIAMADAFGVKLGLEDFVRIAKKVPHLADLKPSGRFVMY
                     DLHKVGGVPAVMKQMLADGYLDGSAMTVTGKTLAENLAGAPGLAAGQTVIRSKDDPLR
                     STGPIVILKGNLAPEGAVAKVGGLSNTTIVGPAKVYDGEESATAAALRGDIRAGDVVV
                     VRYEGPKGGPGMREMLSLTAILSGKGLGDKVGLITDGRFSGGSHGRVVGHVCPEAAVG
                     GPIGLIKNGDTVTINAEKRELSVALSKTEWAKRAKAWKPPKRRATGWLARYAAMVTSG
                     SEGAVLRVPGEGK"
     gene            complement(1456..1992)
                     /locus_tag="PK362_05180"
     CDS             complement(1456..1992)
                     /locus_tag="PK362_05180"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNC74261.1"
                     /translation="MKIYTRVGDGGYTYLFGGNKVRKDNDRVEAYGTVDELNSALGLA
                     AALAKNADVQRRIGAIQRDLFVLGADLATPPKKGAPRVPRIGPAHVRRLEEEIDRMQK
                     DLPPLRHFILPGGSPAGAALHLARAVCRRGERYCASLGRSFPSAIVYLNRLGDYLFVL
                     ARRASRREGKKETLWIPE"
     gene            2057..2701
                     /gene="nth"
                     /locus_tag="PK362_05185"
     CDS             2057..2701
                     /gene="nth"
                     /locus_tag="PK362_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNC74262.1"
                     /translation="MKSSVAPLLRALHRAYPRADCALAHRNPFELLIATILSAQCTDA
                     RVNEVTPVLFGRFPNAEALARAPLGEVETIIRSTGFFRQKALSLLATARRLVEKHRGE
                     VPRSFEALLELRGVARKTANVVMGTAFGEATGVVVDTHVRRLSNRLGLTRESDPVKIE
                     RDLVKRLPRKDWIWYSHALISHGRAVCKAQSPRCPECVLRTLCPFPARSARRVA"
BASE COUNT          508 a          834 c          919 g          507 t
ORIGIN      
        1 ggaaatcatc gccgattcca tcgagacggt ggccggggcc gaacggttcg acggcctggt
       61 cgccatcggc ggctgcgaca agaacatgcc cgggtgcctc atggccatgg gccggctcaa
      121 cattccggcg gtgttcgtat acggcggcag cattcggccg gggtccttcc aagggaaaga
      181 cgtcgacatc gtgagtattt tcgaggcggt gggccagttc aacaataatt taatcgatga
      241 aaagacattg aaaggggtcg agtgttccgc ctgccccggg cccgggtcct gcgggggaat
      301 gtacaccgcc aacaccatgg cgtcggccat cgaatgcctg ggcatgagcc ttccgggcag
      361 ttcgtcctat ccggccgaga cggccgagaa agtggccgag tgcgccgagg ccggcaaagc
      421 cgtggtccag gcccttcggt tgggcctgcg tccccgggac atcgtcaaaa agaagaattt
      481 cgaaaacgcg atcgccttgg tgatggtcct gggggggtcg acgaacgcgg tgctgcattt
      541 gatcgcgatg gccgacgcct tcggcgtcaa attgggtctg gaggatttcg tccgcatcgc
      601 caaaaaagtt ccccatctgg ccgacttgaa acccagcggt cgattcgtga tgtacgacct
      661 gcacaaggtg ggcggcgttc cggcggtgat gaagcaaatg ctggccgacg ggtatctgga
      721 cggttcggcc atgaccgtca cgggaaaaac gttggcggag aacctggccg gggcgccggg
      781 tttggccgcg ggccaaacgg tgattcgttc caaggacgat ccccttcgtt ccacgggacc
      841 gatcgtcatt ttgaagggaa atttggcgcc cgagggcgcc gtggccaagg tggggggatt
      901 gtcgaacacc accatcgtgg gacccgccaa agtttacgac ggcgaagaaa gcgctacggc
      961 cgcggccctt cggggggata ttcgcgcggg cgacgtggtg gtcgtgcgct acgaaggccc
     1021 caaaggcggg ccgggcatgc gcgaaatgct ttccttgacg gcgattttgt ccgggaaggg
     1081 cctgggcgac aaagtcgggc tcatcaccga cggccgtttt tcgggcggaa gccacggtcg
     1141 ggtggtgggc cacgtctgcc ccgaagcggc ggtgggaggt cccatcggtt tgattaaaaa
     1201 cggcgacacg gtcacgatca acgcggaaaa gcgcgaattg agcgttgcgc tgtccaaaac
     1261 ggaatgggcg aaacgggcca aggcctggaa acctccgaaa cggcgggcca ccggttggct
     1321 ggcccgatac gccgccatgg tgacctccgg gtctgaaggc gcggtgcttc gcgttccggg
     1381 cgaagggaag taaaggcgga agggattaat cggtcgacgg tgggacccct gggcggggtc
     1441 gggaaacccg ttgggctatt cggggatcca gagggtttct tttttgcctt cccgtcggga
     1501 ggcccggcgc gccaagacga acaagtaatc ccccagccgg ttcaggtaaa cgatggcgga
     1561 aggaaaagac cggccgaggc tcgcgcaata acgttcgccc cgtcggcaca ccgcccgggc
     1621 caaatgaagg gcggcgccgg ccggcgaccc ccccggcaat atgaaatgac gcaggggcgg
     1681 cagatccttt tgcatccgat caatttcttc ctcgaggcgg cgcacgtggg cggggccgat
     1741 gcggggaacc cggggagccc cttttttcgg gggggtcgcc aaatcggcgc ccagcacgaa
     1801 taagtcccgc tgaatggcgc cgattcggcg ttgaacgtcg gcgtttttcg ccaaggccgc
     1861 cgccagcccc agcgccgaat tgagttcgtc caccgtgccg taagcttcca cccgatcgtt
     1921 gtctttccga accttgttcc cgccaaacag gtaggtgtag cccccgtcgc ccacgcgcgt
     1981 gtagattttc atgggaccca ttttacccct tccaggagct tcggcaaagg ccggtgaaat
     2041 gtgggaaaat agccctgtga aatcttccgt cgccccattg ctccgcgcgc ttcaccgggc
     2101 ctatccccgg gccgattgcg ccttggccca ccgcaacccc tttgaactcc tgatcgcgac
     2161 catcctgtcg gctcaatgca ccgacgcccg cgtgaacgag gtgacgcccg tcctttttgg
     2221 ccggttcccg aacgccgaag cgctggcccg ggctcctttg ggggaagtgg aaaccatcat
     2281 ccggtccacg gggtttttcc gccaaaaggc tctttccctt ctggccaccg cccgccgcct
     2341 ggtggaaaag caccgggggg aggtgccgcg atccttcgag gccttgctgg aattgcgggg
     2401 ggtcgcccgg aaaacggcca acgtggtgat gggcaccgcc ttcggggagg cgaccggcgt
     2461 cgtggtggac acccacgttc gccggctttc gaaccgtttg ggtctcaccc gggagtccga
     2521 tccggtcaag atcgaacggg acttggtgaa acggcttccg cgcaaggatt ggatctggta
     2581 ttcccacgcc ttgatcagcc acggacgcgc cgtgtgcaaa gcccagtcgc cgcgctgccc
     2641 ggaatgcgtg ctgcggactc tctgcccttt tccggccagg agcgcccgtc gtgtcgcttg
     2701 acccccggtt cgtcaaaggc atcgctctgt tcaacaacga ggatttcttc gaggcccacg
     2761 aggcctgg
//
LOCUS       DAOQLS010000226         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21421_length_2767_cov_4.289823, whole genome shotgun sequence.
ACCESSION   DAOQLS010000226 DAOQLS010000000
VERSION     DAOQLS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21421_length_2767_cov_4.289823"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK362_05190"
     CDS             <1..191
                     /locus_tag="PK362_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74263.1"
                     /translation="AFYPGIVIAWLFGSFFPGVSPKQVAPWIYTLWQGFMIALVALLC
                     FLILRPVIGWIGRLLFSH"
     gene            280..657
                     /locus_tag="PK362_05195"
     CDS             280..657
                     /locus_tag="PK362_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74264.1"
                     /translation="MKSKIFLPGVLMMVSFPLFGRGPSVRFSSLSEAISFLGKSLDSM
                     DSGPVQKACVNHRRKWEDNTWKVLLSVHNKTPLNQLYASREFPEGLDAFVLGGHESEL
                     GHVHIDFVKKGKWWSLDSIWMCR"
     gene            1646..2041
                     /locus_tag="PK362_05200"
     CDS             1646..2041
                     /locus_tag="PK362_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC74265.1"
                     /translation="MALAAKIAKSAHNGQYRRDGITPYVRHPENVAARLRGDSTAEAV
                     GWLHDVLEDTGETSQSLRAQGIPEEVIACVEKLTKKEGIDYEQYLATVRRDPIARKVK
                     IADMLSNLSDNPTEKQIVKYSKGLLILLA"
     gene            2117..2506
                     /locus_tag="PK362_05205"
     CDS             2117..2506
                     /locus_tag="PK362_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74266.1"
                     /translation="MKKKSPLFKIAKPSDPALLIHKDDAMGELWAAYVDGEPHWIVKL
                     TGLSYTYPVHPEEPESFLVHIVEEAAHRLRDVVTLKIDPGRIKGLEVKAGTPSEGIKP
                     FVEHLDQWYRGKQYWKDDVAWQNHLAR"
BASE COUNT          583 a          754 c          771 g          659 t
ORIGIN      
        1 tggcctttta tccggggatt gttattgcct ggctttttgg aagttttttc ccgggggtgt
       61 cccccaagca ggtcgccccg tggatctata cgctttggca gggattcatg atcgctttgg
      121 tcgccctgct ctgcttccta atacttcgtc cagtgattgg ttggatcggc cggctacttt
      181 tcagccattg agctcaaccg tgtttcccaa cccatcgctc gaattaccgc tatttgcacg
      241 cgaattgcgc ctccggcgcc cgtcgatggg catcaataaa tgaagagtaa aattttcctg
      301 cctggagttc tcatgatggt ttccttcccc ttatttggca gaggtccatc ggttcgtttt
      361 tcatcgcttt ccgaggcgat ttcctttctc ggcaagtccc tggactccat ggacagcggc
      421 cccgtccaga aggcttgcgt aaaccatcgc aggaaatggg aagacaacac gtggaaagtc
      481 cttctctccg tccacaataa aacacccctc aaccagctct acgcgagtcg ggaatttccg
      541 gaaggccttg acgcctttgt tttaggaggg cacgagtcgg aattgggcca cgtgcatatc
      601 gacttcgtca aaaaaggcaa atggtggtct ttggattcca tatggatgtg caggtaacgt
      661 tttggtgacc gaccatcacc tgaggcagaa aaaaaccggc gtcgatccgg tcgcgccaat
      721 cgcggccggg ggtttcgggt ccggccgagt tcaatggtta ggcaatgata aaaatgatta
      781 aggcaatccc aacttcccta ttggcaaaag accccgccgc gcgggtgggt gggattttgg
      841 cgttcgtttt cctttgcgcg gctggatatg cctttatcca agccgttggg tgctccaaca
      901 ggcgtgtcgg tggcggactg ggcggccttt ggggttgcga tttcggtctt tggttgatga
      961 caactccctg gtcttatctc tttcataaac cgctggagct actgaccgct catcccgtgg
     1021 tcacttattg cgtgtattcc ctattcatag gcctaaatgc cgtcgggatg tatttgatcg
     1081 gcctgggggc gggaagtctg gtccaatgga tcatttctcg gtgagcccct tggcaatggc
     1141 gcggctctcc gttccacccg acgtcggcgc ggcgctcacg ccggggtttt catcggccgc
     1201 gaaatcacga ccaaccacgg aggtttaaat gcgttttgcg gatctgttat ttgccttcac
     1261 cacgctgatg ttcgcggctc cgggtgtttc ggaggaaagg aacttggtcc cttccgcgga
     1321 ggtcgccatc gccatcgcga cagccgtttg gacacctctc tatggagccg aactggtcga
     1381 tcgctatcga ccgtattcgg ctatttcgga gaaaggggct tggatagtaa cgggcactct
     1441 ccccggggga tcaaggggcg gggggtctcc cgaggccacc gtatcaaaga aagacggaag
     1501 gatcacgggt gttcatctgg cacgatagac ccaaccattc catgtcgccg aaaagaatcc
     1561 ccaggaccga tggccgttcg ttccgttaac gccgtcagcg ggaaccacaa atctttttga
     1621 tatgacgaat tctccagacg ccctcgtggc tcttgccgcc aaaatagcca agtcggcgca
     1681 taacggacag tatcggcgcg acggaatcac gccgtatgtt cggcatcccg agaatgtcgc
     1741 ggcgcgcctg cggggggatt cgaccgcgga agcggtcggg tggttgcacg atgtccttga
     1801 agacaccgga gagacgtcgc aatcgctgag ggcccagggt attcccgaag aagtgatcgc
     1861 ctgtgttgag aaactgacga aaaaagaagg cattgattac gaacaatatc tggctacggt
     1921 ccgccgggac cccatcgccc gcaaagtgaa gatcgccgac atgttgtcga acctctccga
     1981 caacccgacc gagaagcaga ttgttaagta ttcaaaaggt ttgttgatat tgcttgcatg
     2041 accgcgtcgc cgcccagcct cgacacaaac gccggaagcg ggcgccgatg aatttgagcc
     2101 gctaggattc gccaacatga aaaagaaaag cccgttgttt aaaattgcca aaccctcgga
     2161 tccggcattg ctgatccaca aagacgacgc catgggcgaa ctgtgggcgg cctatgtcga
     2221 tggggaacct cattggattg tcaagttgac gggactttcc tatacctacc ccgtgcatcc
     2281 cgaggaaccc gaatccttcc tggtccacat tgtggaagaa gcggcccacc gtttaaggga
     2341 cgttgtcacg ttgaaaatcg acccgggacg tataaagggg ctcgaggtta aagcgggcac
     2401 gccgagcgag gggatcaaac cctttgtgga acacctcgac caatggtatc gcggaaaaca
     2461 atattggaag gacgacgtcg cgtggcaaaa tcacctggcg cgctaagccc gggccaatgc
     2521 ccgagcctca tcggcaaatc cgatcgaatc ggacgggccc cggcgcgggg cggctggtta
     2581 tttaattgaa atatttcaaa ttggcccttc tccttgttgc ctcggtgggg tggattgtcc
     2641 ccctttattt ggggatcgac tccataatct cctttctccg cttggaggtg tacccccgcc
     2701 ttctaggcca atcgccgctc aatagttttc cattccttcc tttttcaaag cgcctgatgg
     2761 taattgg
//
LOCUS       DAOQLS010000227         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21440_length_2766_cov_3.375876, whole genome shotgun sequence.
ACCESSION   DAOQLS010000227 DAOQLS010000000
VERSION     DAOQLS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21440_length_2766_cov_3.375876"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK362_05210"
     CDS             <1..277
                     /locus_tag="PK362_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74267.1"
                     /translation="QVVARDDRGGAVTSFHGDVNLNAAVGSVSPAVLSGEHFHEGIAV
                     LSVTLTATNPADRRNRLEAVSRKIVEGQAAPARGGVDLTIKQVEGAR"
     gene            274..>2766
                     /locus_tag="PK362_05215"
     CDS             274..>2766
                     /locus_tag="PK362_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74268.1"
                     /translation="MIRRSRLLATAALALAGASPLWAVSSYIVTAPSDIMIGDSAVTI
                     NVDPREGGAPDGTPHRVKFVGLPAGVTVAPNDLVNGVPIAGPTPFTLFVGAGAVPGVF
                     TLTVQNQVDSAVKGQVPVTIFRTVSQFSITLPGAPFTAMAGVPFQVTLTARDSFGGVV
                     LPFSDNVDISALVGDSSVNGGATFVPGADFVNGVAVATVTLFGTTIGALNNTLTATAQ
                     LLYPGQVTRATGSIGLTVEPNVFDHVLLAFPGETLTPGTGAGKTGAANTATAGISVPV
                     VNAYLVDQFHNPIRTDRNPEPGLYPVSVSFVSITHPGTDTVPAAQLVAAGNQLSMNNA
                     FSFLASGVAHIIQATEATNGTTDSTSISVLSGAAASFEWSAIATPQSAVAPFLATIRA
                     RDAIGNTVTTYNSAAVVSANDCVGTGLGANTVDVDQVTAGVQTSVTFVNGVWTGAVKV
                     TKQKLNVCLNVDDSGGRIGDSNPFRVNAGPLAMLWFTLPGEIYTPGAYPGNALSPNSA
                     TAGDLLTVTIRAVDASWNQVALGGAEPLTLTSATGFIDAPGALVMPAIGDTTVAGVRV
                     RTALPAAQRLAAAITSTNGQSNIFTVNPRPYDRVVIALPGETLAPGVPPATEPDGKTG
                     VVSTQAVNTAFPVSVYLTDDYFNPVVNPPVSGWPSLQFQLTLPVAGTVTFPAPNPYAM
                     AGGTLFNTVTLGNLGVNTFAVTDTLVATKTVTATVNVDPGPLSYFTVSGVANKNAGDA
                     MAITVTAFDQFNNVATNFGGAVRLELWSDGVPVPYDGAIVPSTITFVPNPVTGGVVST
                     NVVVNYAGKQLGLGADRLQIRAYYNLPPLKEGF"
BASE COUNT          448 a          963 c          852 g          503 t
ORIGIN      
        1 tcaagtcgtg gcccgggacg atcggggcgg ggccgtgacc tccttccacg gcgatgtgaa
       61 tttgaacgcc gccgtcggca gcgtctcgcc cgcggtgctt tcgggggagc attttcacga
      121 aggcatcgcc gtcctctccg tcaccttgac ggccacgaac cccgccgatc ggcgaaaccg
      181 attggaagcc gtttcccgca aaatcgtcga aggccaggcg gcgcccgccc gcggcggggt
      241 ggacctgacc attaaacaag tcgagggggc ccggtgattc gccggagccg actcttggcg
      301 accgcggcgc tcgctctcgc gggggcgtcg cccctctggg cggtcagcag ttacatcgtc
      361 accgcgcctt ccgacattat gatcggcgac agcgccgtga ccattaacgt cgacccccgg
      421 gaagggggcg ccccggacgg gaccccgcac cgggtcaaat ttgtgggctt gcccgcgggc
      481 gtgaccgtgg cccccaacga cctggtcaac ggcgtgccca tcgcggggcc cacgcccttc
      541 acccttttcg tcggcgccgg ggccgttccg ggggttttca ccctcaccgt tcaaaaccag
      601 gtggactcgg ccgtcaaggg ccaggttccc gtgaccattt tccggacggt ttcccaattt
      661 tcgatcacgc tgccgggggc gccttttacg gccatggccg gggtcccctt ccaagtgacc
      721 ctgaccgccc gggactcctt tggcggcgtc gtcctgccct tttcggacaa cgtcgatatc
      781 tcggctttgg tgggggacag ttcggtcaac ggcggcgcca ccttcgtgcc gggggcggat
      841 tttgtgaacg gcgtggccgt ggccaccgtg actctttttg gaacgaccat cggggctctc
      901 aacaacaccc tcaccgccac cgcccagctc ttgtatccgg gccaggtcac ccgggcgacg
      961 ggctccatcg gattgaccgt cgagccaaac gtgtttgatc acgtcctgtt ggcgtttccg
     1021 ggggaaacct tgacgcccgg caccggggcg ggaaaaacgg gcgccgcgaa cacggccacg
     1081 gcggggatct ccgtgccggt ggtgaacgcc tacctggtgg accaattcca caatcccatt
     1141 cggaccgacc gcaaccccga gccgggactt taccccgttt ccgtgagttt cgtgtccatc
     1201 acccaccccg gcaccgacac cgtgccggcc gcgcagttgg tggcggcggg gaaccaactg
     1261 tccatgaaca acgccttctc ctttttggct tcgggcgtgg cccacatcat ccaagccacg
     1321 gaggccacca acggaacgac ggactccacc tccatttcgg tcttgagcgg cgccgccgcg
     1381 agttttgaat ggtccgccat cgcgaccccg cagtcggccg tggcgccttt tctggcgacc
     1441 atccgcgccc gggacgccat cggcaacacc gttaccacct acaattcggc ggccgtcgtg
     1501 tcggcgaacg actgcgtcgg cacgggcctg ggcgccaaca ccgtcgacgt ggaccaggtc
     1561 accgccgggg tccagacgag cgtcactttc gtcaacggcg tttggaccgg ggccgtcaaa
     1621 gtgaccaaac aaaaactcaa cgtttgcttg aacgtggacg acagcggggg gcggatcggc
     1681 gattcgaacc cgttccgggt caacgccggg cctttggcca tgctttggtt cacgttgccc
     1741 ggagaaattt acacgccggg cgcctacccc ggcaacgccc tttcgcccaa cagcgccacc
     1801 gccggcgatt tgctcacggt caccattcga gcggtggacg ccagctggaa ccaagtggcc
     1861 ttgggcgggg ccgaacccct gacgttgacc tcggccaccg ggtttatcga cgcgcccggc
     1921 gctctcgtca tgccggccat cggcgacacc accgtggcgg gggttcgggt tcgcacggcg
     1981 ttgccggcgg cccaacggtt ggcggccgcc atcacctcca ccaacggcca gagcaacatt
     2041 ttcaccgtga acccgcgccc ctacgatcgg gtcgtgatcg cgcttccggg ggagacgctg
     2101 gcccccgggg ttccgccggc gacggaaccc gacgggaaaa cgggggtcgt ttccacccag
     2161 gcggtcaaca ccgcctttcc cgtttcggtg tatctgaccg acgactattt caaccccgtg
     2221 gtgaaccccc ccgtttccgg gtggccttcc cttcaatttc agttgacgct gccggtggcg
     2281 ggcaccgtga ctttcccggc ccccaatccc tacgcgatgg ccggcggtac cttgtttaac
     2341 accgtgaccc tgggcaacct gggcgtcaac acctttgccg tgacggacac cttggtcgcc
     2401 accaaaacgg tgaccgcgac ggtgaacgtg gatcccggtc cgttgtccta tttcacggtg
     2461 tccggcgtgg ccaacaaaaa cgcgggcgac gccatggcca tcacggtgac ggccttcgat
     2521 caattcaaca acgtcgcgac caatttcggc ggcgcggtgc ggctggagct gtggagcgac
     2581 ggcgtgcccg tgccctacga cggggcgatc gtcccctcca ccatcacttt cgtgcccaac
     2641 cccgtgaccg gcggcgtggt ttcgaccaac gtggtcgtca attacgccgg aaaacagttg
     2701 ggcctcggcg ccgaccgtct gcaaatccgg gcctactata atttgccgcc tttgaaagag
     2761 ggcttc
//
LOCUS       DAOQLS010000228         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21449_length_2765_cov_4.947601, whole genome shotgun sequence.
ACCESSION   DAOQLS010000228 DAOQLS010000000
VERSION     DAOQLS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21449_length_2765_cov_4.947601"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PK362_05220"
     CDS             complement(<1..160)
                     /locus_tag="PK362_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HNC74269.1"
                     /translation="MKTHAGRKLSRPTGARVALLRGLATSLLQHEEIRTTHAKAKEAA
                     RFAEGLLAL"
     gene            complement(157..1137)
                     /locus_tag="PK362_05225"
     CDS             complement(157..1137)
                     /locus_tag="PK362_05225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNC74270.1"
                     /translation="MTSLGLVLPQKLDTDAASSDNYGKFVAEAFEKGYGHTVGHSLKR
                     ILMSSLEGAAVTGVRIKGAPHEFSSLKGVQEDVIKLILNLRQLRFKLHVAGPEVLTLK
                     KKGGEVKAKHIELTSNVELLNPDLVLAHLDAGGELDMEIEVSRGRGYSSADRLRREGQ
                     PVNTIPVDALFSPVTKVHYEIENARVGQMTDYDKLILEVWTDGSITPMAAVAQSAKIL
                     KNAFGVFAPAEPAPASEPATAAQGLAADGQGRLKDMMDQSVDMIELTVRASNCLKAAK
                     IRTIGDLVSKTEEELLGFKNFGRKSLEEIRDRLKELNLELGMLTAGGEPK"
     gene            complement(1172..1855)
                     /gene="rpsD"
                     /locus_tag="PK362_05230"
     CDS             complement(1172..1855)
                     /gene="rpsD"
                     /locus_tag="PK362_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNC74271.1"
                     /translation="MARYTGPVCKLCRREGVKLFLKGDRCFSKCPIDKEGAAVPPGQH
                     ARRRGTKPTEYAKRLREKQKARRMTGVLERQFRRLFDAASHQKGNTGENLLRLLETRL
                     DNVVRRLGFSTSPAAARQLVFHGHVKVNGQRVTIPSYQVEPGDAIALDDALRANLFVK
                     RSLATAAQKGLPGWLEWDGSIGGAVKGHADNVSLDGVTLAGKVKNWPARAEMSFPVNE
                     QFIVELYSK"
     gene            complement(1874..2326)
                     /gene="rpsK"
                     /locus_tag="PK362_05235"
     CDS             complement(1874..2326)
                     /gene="rpsK"
                     /locus_tag="PK362_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNC74272.1"
                     /translation="MADQPEAKKDAAKAAAKPAAKAPASAPKGKKKIWRDIVNARVYI
                     QSSFNNTIVNITDERGNAIVWATAGSAGFKGTKKGTPFAAQVTADAAAKKAVALGVKS
                     VSVFVKGPGSGRETAIRALQAAGLVVTSIKDVTPIPHDGCRPPKARRV"
     gene            complement(2345..2749)
                     /gene="rpsM"
                     /locus_tag="PK362_05240"
     CDS             complement(2345..2749)
                     /gene="rpsM"
                     /locus_tag="PK362_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNC74273.1"
                     /translation="MARVAGIDLPKQKRVDVALRYLYGIGAHRSLLICGETGVNPSTR
                     VKDLTEAEVNKINTELTKNYKVEGDLRREINGTIRRLIEIGSYRGSRHRRNLPARGQR
                     TKSNARTRRGRRRTVGSAKPGTTAAPATAAAK"
BASE COUNT          410 a          834 c          934 g          587 t
ORIGIN      
        1 ccagggccag caggccttcc gcgaaacgcg cggcttcctt ggctttggcg tgggtcgtgc
       61 ggatctcctc gtgctggagg aggctggtgg ccaggccgcg cagcagggcg acccgggcgc
      121 ccgtggggcg cgagagtttt cgtccggcgt gggtcttcat ttgggctccc cgccggcggt
      181 cagcatgccc agctccaggt tcaattcttt aaggcggtcc cggatttcct ccaaggattt
      241 ccggccgaag ttcttaaagc ccagcaattc ctcttcggtc ttggacacca ggtcgccgat
      301 cgtgcggatc ttggcggcct tcaggcagtt ggaggcgcgc acggtcagct cgatcatgtc
      361 gacggattgg tccatcatgt ccttgaggcg cccttggccg tcggccgcga ggccttgggc
      421 ggcggtcgcc ggttcggacg ccggcgcggg ttcggccggg gcgaaaacgc cgaaggcgtt
      481 cttgagaatc ttggccgact gggccacggc ggccatgggc gtgatggagc cgtcggtcca
      541 cacttcgagg atcagcttgt cgtagtcggt catctgaccg acgcgggcgt tctcgatttc
      601 gtagtggacc ttggtcaccg gggagaagag cgcgtccacg ggaatggtgt tgacgggctg
      661 gccttcgcgg cgcagacggt cggcggagga gtagccccgg ccgcgggaca cttcgatctc
      721 catgtccagc tcgccgcccg cgtccaggtg ggccaggacg aggtcggggt tgagcagctc
      781 gacgttgctg gtgagctcga tgtgcttggc tttgacttcg ccgccctttt tcttcagggt
      841 cagcacttcg gggcccgcca cgtggagctt gaagcgcagc tggcggaggt tcaagatcaa
      901 cttaataacg tcttcctgaa cgcctttgag gctcgagaat tcgtggggcg cgcccttgat
      961 gcggacgccc gtgaccgcgg cgccctccag ggacgacatc aaaatgcgtt tcagggaatg
     1021 gcccaccgtg tggccgtagc ctttttcaaa cgcttccgcg acaaacttac cgtagttgtc
     1081 cgaggaagcc gcgtccgtat ccagcttctg gggcaacacc aagccaagac tcgtcatgag
     1141 aattctctcc ttcttgaaaa agcgtccggg gttatttgct gtaaagttcg acgatgaact
     1201 gttcgttgac ggggaacgac atctccgcgc gcgcgggcca gtttttcact ttgccggcga
     1261 gggtgacgcc gtccaggctc acgttgtccg cgtggccctt cacggcgccg ccgatggaac
     1321 cgtcccattc caaccagccg ggcaggccct tttgggccgc cgtggccaga gagcgcttca
     1381 cgaacaggtt ggcgcgcagg gcgtcgtcca gggcgatcgc gtcgccgggc tccacctggt
     1441 aggacgggat ggtgacccgt tggccgttca ctttgacgtg gccgtggaac acgagctggc
     1501 gcgccgccgc gggcgacgtg gagaaaccga ggcgtcggac cacgttgtcc agtcgggttt
     1561 ccaacaaccg gaggaggttt tccccggtgt tgcccttctg gtgggacgcc gcatcgaaca
     1621 aacggcgaaa ttggcgttcc aacacgccgg tcatccggcg cgctttctgc ttctcgcgca
     1681 gacgcttggc gtattccgtg ggtttggttc cccggcggcg ggcgtgctgc cccggcggta
     1741 ccgcggcgcc ttccttatcg atcgggcact tcgaaaagca ccgatcgccc ttcaaaaaaa
     1801 gcttcacgcc ttcgcggcgg cagagcttgc aaacgggtcc agtgtatcga gccacaacgt
     1861 tctccttggg gggttagacg cggcgggcct tgggaggccg gcaaccgtcg tggggaatgg
     1921 gggtcacgtc tttgatggag gtcaccacca agcccgcggc ttgaagcgcc cggatggcgg
     1981 tttcccgtcc cgaccccgga cctttgacga acacggacac gctcttcacg ccgagcgcga
     2041 ccgccttttt ggcggcggcg tccgcggtga cttgcgcggc gaacggcgtg ccttttttgg
     2101 tgcctttgaa cccggcgctg cccgccgtgg cccagacgat ggcgttgccg cgctcgtcgg
     2161 tgatgttgac gatcgtgttg ttgaaggacg attgaatgta cacgcgcgcg ttgacgatgt
     2221 cccgccaaat ctttttcttg cctttggggg cggacgccgg ggctttggcg gccggtttgg
     2281 ccgcggcttt ggcggcgtct ttcttcgctt cgggttgatc agccatggat tctcctcggt
     2341 aaacctattt cgccgcggcg gtggccgggg cggcggtggt gccgggcttc gcggagccga
     2401 cggtgcgccg tcgcccgcgc cgggtgcgcg cgttcgattt cgttcgttgg ccccgggccg
     2461 gcaggttgcg ccggtgccgc gagccgcggt aggaaccgat ttcgatgagg cggcggatgg
     2521 tgccgttgat ttcccggcgc aaatcgcctt ccactttgta gttcttggtc aattcggtgt
     2581 tgatcttgtt gacttccgcc tcggtcaaat ccttgacccg ggtcgacgga ttgacgccgg
     2641 tttcaccgca gatgagaagg gagcgatggg ccccgatgcc gtaaaggtac cgcaacgcga
     2701 catcaacgcg tttttgtttg ggaagatcga ttcccgcgac gcgagccatg tgagtcctcc
     2761 ttaac
//
LOCUS       DAOQLS010000229         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21675_length_2749_cov_3.325909, whole genome shotgun sequence.
ACCESSION   DAOQLS010000229 DAOQLS010000000
VERSION     DAOQLS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21675_length_2749_cov_3.325909"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..>2749)
                     /locus_tag="PK362_05245"
     CDS             complement(195..>2749)
                     /locus_tag="PK362_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNC74274.1"
                     /translation="TVYPVNPKRPSVLGIKAYPRVSDIPEAVDLAVIATPAPSVPDVV
                     SECVSLGVPGGIVISAGFKEYGAEGASLEKRLADAARGKMRLLGPNCLGLMCPPSGLN
                     ATFAKGISAAGNVGFLSQSGALCTAVLDWSFRENIGFSVFASLGSMLDIGWGDLIDYL
                     GNDPKTESIVIYMESVGDARAFMSAAREVSLTKPIIVIKAGRTDAAAKAAVSHTGSLV
                     GRDDVLDAAFERCGVVRVDSISELFQMAATLSKQPRPKGPRLTIVTNAGGPGVLAADA
                     LIEHGGQLAELAETTVKEMSGYLPGPWSHGNPVDVLGDADASRFGRAFESAAKDPNSD
                     GLLVILTPQAMTDATGSAQHVALKVGAAPGKPVLASWMGGDTVADGRQILTGAGIPVF
                     NYPDTAARIFCTMWSYSANLRALYETPTLSAGADAAGRDRTGVEHLLAKARGEGRVLL
                     SEAESKALLAAYGIPTVDTRVAATAEEAVKAAEAIGYPVVLKLHSHTVTHKSDVGGVR
                     LSIADAAAVRGAFAAIQRGVEAAKGPGHFLGVTVQPMVRAEGVEVILGASPDSQFGPV
                     LLFGTGGKMVEVYKDRALGLPPLTTTLARRLMEKTKVWKALQGVRGDRAADIAALEHA
                     LVRFSWLVAEQDAIKEIDINPLVVSPTGVVALDARVVLYGPTEPPRVRPAIRSYPAEH
                     VRSFTAKDGTGLVIRPVRPEDEPALVKFHEELSERSVVSRYLRPFEFSERTAHDRLRR
                     VCFTDYDREMVFVAAVPKPAGEDIWAVGRLSRRPGGREAQFSMLVSDPHQKIGLGGGL
                     LAHLIEVGRAEGLTALWADIRADNVEMLKVAQRLGFRVLSCTDGTGRAHLDL"
BASE COUNT          456 a          927 c          942 g          424 t
ORIGIN      
        1 ccgccacggc cgtccgcagg tccgagagga tccgccccga ggcccgggcc cggggatagg
       61 cgccccgtcc ggcgctggcc ccgacgcgtt cttgatagcg ggccaagctt tgggccaccc
      121 gcgggggttt gggccaggag gtcgcggcgt tgtcgaagta aatcggcgaa ggcacgaagg
      181 tcccgcgccg agaattacag atccaggtgc gcccggccgg tgccgtccgt gcaggacagg
      241 acccggaacc ccaggcgttg ggccaccttt aacatttcga cgttgtcggc ccgaatgtcg
      301 gcccaaaggg ccgtcaagcc ctcggcccgt cccacctcga tcaaatgggc cagaagaccg
      361 ccgcccaagc cgattttctg gtggggatcg gagacgagca tggaaaattg ggcttcccgt
      421 cccccgggac gccggctcaa ccggcccacg gcccaaatgt cctcgccggc cggtttcggc
      481 acggcggcga cgaagaccat ttcccggtcg tagtcggtga aacagacccg gcgcagacgg
      541 tcgtgggcgg tccgctcgga aaattcaaag gggcgcaggt aacgggagac gacgctccgc
      601 tccgacaatt cctcgtgaaa tttcacgagg gccggttcgt cctcgggccg gacgggacgg
      661 atcaccaacc ccgtgccgtc cttggcggtg aaggaccgga cgtgttccgc cgggtaggac
      721 cggatggccg ggcggacccg gggcggctcc gtcggtccgt agaggaccac gcgggcgtcc
      781 agggccacga cccccgtggg cgaaaccacc agggggttga tgtcgatttc cttgatggcg
      841 tcctgctcgg cgaccagcca gctgaaacgg accagggcgt gctccagggc ggcgatgtcg
      901 gccgcccggt ccccgcgcac gccttgaagg gccttccaca ctttcgtctt ttccatcaac
      961 cggcgggcca aggtcgtcgt caagggcggc aaccccaggg cccggtcctt gtaaacctcg
     1021 accatttttc cgccggtgcc gaaaagcaac acgggaccga actgggaatc ggggctggcc
     1081 cccaaaatga cttccacgcc ctcggcccgg accatgggct ggacggtgac gcccaggaaa
     1141 tgaccgggcc ccttggcggc ctccaccccg cgctgaatgg cggcgaaggc cccccggacc
     1201 gcggcggcgt cggcgatgct gaggcgcacg ccgcccacgt cggacttgtg ggtcaccgtg
     1261 tgggaatgga gtttcaacac caccggatac ccgatggcct cggccgcctt cacggcctcc
     1321 tcggccgtgg cggccacccg cgtgtcgacg gtcggaatgc cgtaggccgc caacagcgct
     1381 ttggactcgg cttcggacaa aaggacgcgc ccttcgcccc gggccttggc caggaggtgc
     1441 tcgacccccg tgcggtcccg gcccgcggcg tcggccccgg cggacagggt cggcgtttcg
     1501 tagagcgcgc gcaggttggc gctgtaggac cacatggtgc agaaaatccg ggcggccgtg
     1561 tcgggatagt tgaaaacggg gattccggcg ccggtcaata tctggcgtcc gtcggccacg
     1621 gtgtcgcccc ccatccaact ggcgaggacc ggtttccccg gggcggcgcc caccttgagg
     1681 gccacgtgct gggccgaccc ggtggcgtcc gtcatggcct ggggcgtcaa aatcaccagc
     1741 agtccgtcgg aattcgggtc cttggcggcc gattcaaagg cgcgcccgaa gcggctggcg
     1801 tccgcgtcgc ccaggacgtc cacggggttg ccgtggctcc agggcccggg aagatacccg
     1861 ctcatttcct tcacggtcgt ttccgccaat tcggccagtt ggccgccgtg ttcgatcagc
     1921 gcgtcggcgg cgagcacgcc cggaccgccg gcgttggtca cgatggtcag gcgggggccc
     1981 ttggggcgcg gctgtttgga cagcgtcgcc gccatttgaa agagttcgga aatggaatcc
     2041 acccgcacga ccccgcaccg ttcaaaagcg gcgtccaaca cgtcgtcccg cccgaccagg
     2101 gaccccgtgt ggctcacggc cgccttggcc gccgcgtccg tgcggccggc tttgatgacg
     2161 atgatgggct tggtcaggga cacttcccgg gccgccgaca tgaaagcccg ggcgtcgccc
     2221 acggactcca tgtagatgac gatgctttcg gtcttgggat cgttgcccag gtaatcgatg
     2281 aggtcgcccc agccgatgtc cagcatggac cccagggagg cgaaaacgga aaaaccgatg
     2341 ttctcccgga aactccaatc caacacggcg gtgcacaggg cgccggactg gctcaggaaa
     2401 cccacgttgc cggccgccga aatgcccttg gcgaaggtgg cgttcaaccc ggagggcggg
     2461 cacatgagtc ccaggcagtt ggggcccaga aggcgcattt tgccccgggc cgcgtcggcc
     2521 agacgttttt ccagggaggc gccttcggcg ccgtattcct taaacccggc ggaaatcacg
     2581 atgcccccgg gcacgcccag ggagacgcat tcggacacga cgtccggcac cgagggggcg
     2641 ggcgtggcga tgacggccaa atccacggcc tccggaatgt ccgagacccg ggggtaggcc
     2701 ttgatgccca ggacgctcgg gcgtttgggg ttcacggggt aaaccgtcc
//
LOCUS       DAOQLS010000230         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21696_length_2747_cov_3.451337, whole genome shotgun sequence.
ACCESSION   DAOQLS010000230 DAOQLS010000000
VERSION     DAOQLS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21696_length_2747_cov_3.451337"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PK362_05250"
     CDS             complement(<1..617)
                     /locus_tag="PK362_05250"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNC74275.1"
                     /translation="MMKAKICGITNEEDATWAVNLGAHFIGLNFYSESPRKVSVELAG
                     RIAKKVPPFVPLVGVFVNQTPAEIVKIAQKVGLGGIQLHGDHTPDDCRAIAGQLESFV
                     LRAFRIGGEADLEPLAGFKEVCKYFLLDARVEGQPGGTGQTFPWELAARAKSFGVPIF
                     LAGGLTPENVEAAVEAVQPFAVDVASGVEKSPKRKDYDKMKKFLEV"
     gene            complement(614..1408)
                     /gene="trpC"
                     /locus_tag="PK362_05255"
     CDS             complement(614..1408)
                     /gene="trpC"
                     /locus_tag="PK362_05255"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNC74276.1"
                     /translation="MSETKGLDRIVARVRAALPEKQRAVPFAVLEKRAQEKPRPLDFL
                     SAVRQPDRVSVIAELKKASPSGGLLRADYDVAAGAKAYAAAGARALSILTEENYFLGE
                     PDHLEKAKAASGLPVLRKDFVVDPYQVHEARAFGADAVLLIVALLEDKTLRALLSIVK
                     EWGMTPLVEAHDALELRRAVDVGAQLIGINSRNLKDLTMNPGAFESLLPRVPPGVTAV
                     AESGLKTAEDIKKVRALGARAVLVGESLLRQNDLESAARILVEAGQ"
     gene            complement(1405..2436)
                     /gene="trpD"
                     /locus_tag="PK362_05260"
     CDS             complement(1405..2436)
                     /gene="trpD"
                     /locus_tag="PK362_05260"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNC74277.1"
                     /translation="MIVPAIAKLVDRKDLTFDEARESVAEIFGGHVPPSQIAGFLIAL
                     RMKGETVEEIAGAAEAMRSAATRIHLPDVSVIADTCGTGGDKQGSFNISTAAAFVVAG
                     AGFTVAKHGNRSISSQCGSADVLEVLGVRVDPAPSVVERCLKEIGIGFLFAPAFHPAM
                     KFAGPVRRELGVRTVFNVLGPLTNPAGANAQVVGVFSPRMVEVLAKVLVRLGTRESMV
                     VHGAGHDEITLSGNTTVADIRGGRITHRTLTPKDFGFKRVPASVLVGGDKETNADIVR
                     RVLKGEPGPQRDAVVANAAAALWVAGRAAGRADLKTLLHGRRAAEASIDTGAAREKLE
                     RLAVLSNTP"
     gene            complement(2433..>2747)
                     /locus_tag="PK362_05265"
     CDS             complement(2433..>2747)
                     /locus_tag="PK362_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNC74278.1"
                     /translation="NKQIAGFRFRRQHPLGRFVADFVCLDRCLVVEVDGGQHAESARD
                     DQRSSDLNKMGFVVLRIWNNEVFKNLDAVKEKILMALISNKGTPHLNPPPQGGRKSEA
                     MR"
BASE COUNT          496 a          906 c          834 g          511 t
ORIGIN      
        1 acttccaaaa attttttcat tttgtcgtaa tccttccgtt tgggggattt ttcgaccccg
       61 ctggccacgt ccaccgcgaa gggctggacg gcttcgacgg cggcctccac gttctccggc
      121 gtcagcccgc ccgccaagaa aatcggaacc ccgaaggatt tggcccgggc cgccagttcc
      181 cacggaaagg tttgccccgt gccgccgggc tggccctcca cgcgggcgtc cagcagaaaa
      241 tatttgcaca cctctttaaa cccggccagc ggttccaaat cggcttcgcc gccgatgcgg
      301 aaggcccgca ggacgaaact ttcgagctgg ccggcgatgg cgcggcagtc gtccggcgtg
      361 tggtccccgt gaagctgaat tcccccgagg ccgactttct gggcgatctt gacgatttcc
      421 gcgggggtct ggttcacgaa cacgcccacc aagggcacga agggcgggac ttttttggcg
      481 atccggccgg ccagttccac ggacactttc cgggggcttt ccgaataaaa attgagtccg
      541 atgaagtggg cgcccaggtt cacggcccag gtcgcgtctt cttcgttggt gatgccgcaa
      601 atcttcgcct tcatcattgt cccgcctcca cgagaatccg cgccgcggat tccaagtcgt
      661 tttgccgaag cagggattcc cccaccagca cggcccgggc cccgagggcc cgcacttttt
      721 tgatgtcctc cgccgttttg agaccgcttt ccgccacggc ggtcacgccc ggcggcaccc
      781 ggggaagcag cgattcaaaa gccccggggt tcatcgttaa atccttcaaa ttccgactgt
      841 tgattccgat cagttgggcg ccgacgtcca ccgcccggcg caattcgagg gcgtcgtggg
      901 cttccaccaa gggcgtcatg ccccactcct tgacgatcga gaggagcgcc cgaagggttt
      961 tgtcctccag cagggcgacg atcaaaagaa cggcgtcggc tccgaaagcc cgggcctcgt
     1021 ggacctggta aggatcgacg acgaaatcct tccgcaaaac gggcaggccg gaagccgcct
     1081 tggctttttc caggtggtcc ggctccccca gaaaataatt ttcctccgtc aatatcgaga
     1141 gggcccgggc cccggcggcg gcgtaagctt tcgcccccgc ggccacgtcg taatcggccc
     1201 gaagaagccc cccggagggg ctcgcttttt tgagctcggc gatgaccgac acgcgatccg
     1261 gctggcggac ggccgaaagg aagtcgagcg gacgcggttt ttcctgggcc cgcttttcga
     1321 ggacggcgaa cggcacggcc cgctgttttt ccggcagggc ggcccgcact cgggcgacga
     1381 ttcggtccag ccctttcgtt tcgctcacgg ggtgttggaa aggacggcca gccgctccag
     1441 cttttcccgg gcggcgccgg tgtcgatgga ggcttcggcc gcgcggcggc cgtgcaatag
     1501 ggtttttaaa tcggcccggc ccgcggcgcg gcccgccacc caaagggccg ccgcggcgtt
     1561 ggccaccacc gcgtcccgct gggggcccgg ctcgcccttc aacacgcggc gaacgatgtc
     1621 ggcgttggtt tccttgtccc cgcccaccag gaccgacgcg gggacccgct tgaaaccgaa
     1681 atccttgggc gtcaacgtgc ggtgggtgat gcgtccgccg cgaatatccg ccacggtcgt
     1741 attcccggaa agggtgattt cgtcgtggcc cgcgccgtgg acgaccatgg attcccgggt
     1801 gcccagtcga accaggacct tggccaacac ctccaccatg cgcggggaga aaacgcccac
     1861 cacctgggcg ttggccccgg cggggttggt caagggtccc agcacgttga aaacggtccg
     1921 aacgcccaac tcccgacgaa cgggcccggc gaatttcatg gcgggatgga aggccggggc
     1981 gaaaagaaac ccgataccga tttcctttaa gcaccgttcg accaccgagg gggccgggtc
     2041 cacccgcact cccaggacct ccagcacgtc cgcgctcccg cactgggagg aaatggaacg
     2101 gttgccgtgc ttggccacgg tgaaaccggc cccggccacc acgaaagcgg cggccgtcga
     2161 aatattgaag ctgccttgct tgtccccccc ggtgccgcag gtgtcggcga tcacggaaac
     2221 gtccggcagg tggatgcgcg tggccgccga ccgcatggcc tccgccgccc cggcgatctc
     2281 ctccaccgtc tcgcccttca tgcggagggc gatcaaaaaa ccggcgatct ggctcggggg
     2341 gacgtggccc ccgaaaatct cggccacgga ttcccgggct tcgtcgaagg tcagatcctt
     2401 tcgatcgacc agtttggcga tggcgggaac gatcatcgca tggcctctga tttcctcccc
     2461 ccttgagggg gaggattcag gtggggggtg cctttatttg aaatcaaagc cataagaatc
     2521 ttttccttca ccgcgtccag gttcttgaat acctcgttat tccaaattcg taaaacgaca
     2581 aaacccattt tattcaagtc cgaagacctt tggtcgtccc gcgccgattc ggcgtgttgg
     2641 ccgccatcca cctcaacgac caaacaccgg tcaaggcaaa cgaaatcggc gacaaatcgt
     2701 cccagaggat gttgacggcg gaaccgaaaa cccgcgatct gtttatt
//
LOCUS       DAOQLS010000231         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21774_length_2741_cov_3.422189, whole genome shotgun sequence.
ACCESSION   DAOQLS010000231 DAOQLS010000000
VERSION     DAOQLS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21774_length_2741_cov_3.422189"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="PK362_05270"
     CDS             <1..1806
                     /locus_tag="PK362_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNC74279.1"
                     /translation="WPAWTRRLFPGFPFAFHAPISFVLSAIPSALVPRRFPHLIALLT
                     LGFAGVVARLAFVQLWCHSEFSRRVLQQSSRWLNEAPRRLPIVDRNGAVLAESVRVAS
                     CYADPRAMRRPEATVQRLAALLHLPPGPLLTKIRESRNAFVWVKRFLSAEETQALERE
                     KGEGVGLRWEYHRSYPNGSLASSLLGYVGEDGQGLSGMEYAFEADLVDRRAPRRAFRD
                     GRGERVALGEDETAPSETGHLRLSIDRSLQYIAERELEAGMARSRSRGGMILVQDPRT
                     GELLAFANRPALPRGGADAPSAEELGIPAVQWVFEPGSTFKLVTAAAALEEGVVSPRE
                     AFNCEGGRWKYSSITINDHQPEKTISFARAMEVSSNIGLAKVGLRVGPEKLFDYIRAF
                     GFGVRAGSELAGESPGLLRPLRQWSGASLPVVSFGQEVGVTALQLAGAYSAVANGGTL
                     VEPRLCLDLQRPDGSSRQWPLGAAVRRVVSGDTATTLSNLLRGVVDRGTGRDAALGGW
                     TVAGKTGTAQKTNPATRTYFPDRFVASFCGYAPASRPRLTIVVVFDEPKGIAWGGYNA
                     GPVFRNVAWQSLIKMGVPPDQTDRLAERPRGGGRT"
     gene            1803..>2741
                     /locus_tag="PK362_05275"
     CDS             1803..>2741
                     /locus_tag="PK362_05275"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNC74280.1"
                     /translation="MTSFRSLLEGHAVRWAGPAVDADLRGLACDSRRVRPGDLFVALR
                     GHRVDGHRHAAAAVRAGAAALLVEEPVGESVPTAVVSSTDKILSTVAARFYGHPSRSL
                     RVLGVTGTNGKTTITYMMETLLRAAGRPAGVLGTIEYRWPGHAEPAVNTTPMAADVQR
                     LLAGMKAAGVTDVAMEVSSHSLALGRVEDVSFSVGVFTNLTQDHLDFHKDMERYFNAK
                     ARLFDLLARPEGPVGRRAVLNGDDPWTPRFMERLAVPRWTYALDRPADIGAASLALNA
                     VGSRFELRTPVGAAPVALHLVGRHNVYNALAAAGGGL"
BASE COUNT          374 a          932 c          933 g          502 t
ORIGIN      
        1 tggcccgctt ggacgaggcg gctctttccc ggttttccct tcgccttcca cgccccgatc
       61 agcttcgttt tgtccgcgat tcctagcgcc ctcgtgcccc gccgcttccc ccatctcatc
      121 gcgctgttga ctctcgggtt tgcgggcgtc gtggcccgct tggctttcgt tcaattgtgg
      181 tgccactcgg aattttcacg gcgggtgctt cagcaatcga gccgatggct gaacgaagcc
      241 ccccgccgcc ttcccatcgt ggatcgcaac ggcgccgtgt tggccgaatc cgtgcgcgtg
      301 gcttcctgct acgcggaccc ccgcgcgatg cggcgtccgg aggccaccgt ccagcgcctg
      361 gccgccctgc tccaccttcc ccccggtccc ctgttgacca aaatccggga atcgcgaaac
      421 gccttcgtgt gggtgaaacg atttttgtcc gccgaggaaa cccaggcgct ggaacgggag
      481 aagggggagg gcgtcggtct tcgatgggag taccatcggt cgtatcccaa cggctccttg
      541 gcttcgtcgc tcctggggta cgtgggagag gacgggcaag ggctctcggg gatggaatac
      601 gcttttgagg ccgacctggt ggaccgccgc gccccccggc gggcctttcg tgacggccgc
      661 ggcgagcggg tggccctggg cgaggacgag accgctccct cggaaaccgg ccatttgcgg
      721 ttgtccatcg accgatccct ccaatacatc gcggagcggg agctggaggc cggcatggcc
      781 cgttcccgtt cccggggggg gatgatcctg gttcaggatc ctcgcacggg ggaattgctg
      841 gccttcgcca accggccggc cctgccccgg ggcggcgccg acgccccctc cgccgaagag
      901 ctgggaatcc ccgccgtgca gtgggtgttt gaacccggtt ccacgttcaa gctcgtgacc
      961 gcggcggccg ccttggaaga aggggtggtg tcgccgcgcg aggcctttaa ttgcgaaggg
     1021 gggcgctgga aatattcgtc catcaccatc aacgaccatc agccggaaaa aacgatttcc
     1081 tttgcgcggg cgatggaggt gtcctccaac atcgggttgg ccaaagtggg ccttcgcgtg
     1141 ggtccggaga agctcttcga ctacatccgc gccttcgggt tcggcgtgcg ggccgggtcc
     1201 gaactggcgg gggaaagccc gggtttgctg cgtcccctcc gtcaatggag cggggcctcg
     1261 cttcccgtcg tgtcctttgg gcaggaggtc ggggtgaccg ccctccaatt ggcgggggcc
     1321 tacagcgccg tggccaacgg gggaaccctg gttgaaccgc ggctgtgttt ggaccttcag
     1381 cggccggacg gttcgtcccg gcaatggccg ctcggcgccg ccgtccggcg ggtcgtttcc
     1441 ggcgacacgg cgaccaccct ttcgaacctg ctgcgcggcg ttgtggaccg gggaaccggc
     1501 cgggacgcgg ccttgggtgg atggaccgtg gccggcaaaa ccggcaccgc ccaaaaaacc
     1561 aaccccgcca cccgaaccta ctttcccgac cggttcgtgg cgtccttttg cgggtatgcc
     1621 cccgcttccc ggccccgcct gacgatcgtc gtcgttttcg acgagcccaa gggaatcgcc
     1681 tggggcggct acaacgccgg acccgtgttt cgcaacgtgg cctggcagag tttgattaaa
     1741 atgggcgtcc ccccggacca aaccgaccgg ttggcggagc ggccccgcgg cggggggcgc
     1801 acgtgacgtc ctttcgttcg ttgttggagg gccacgccgt gcgttgggcg gggccggccg
     1861 tcgacgcgga tttgcgcgga ctcgcttgcg attcccgacg cgttcggccg ggggacctct
     1921 tcgtcgccct tcggggccac cgcgtggacg gccaccgcca cgcggccgcg gcggtgcgcg
     1981 ccggcgccgc ggccctcctg gtcgaagagc ccgtcgggga aagcgtgccc acggcggtgg
     2041 tctcctccac cgataaaatt ctgtcgaccg tcgccgcgcg tttttacggc catccctccc
     2101 ggtccctccg cgtgttgggc gtgaccggga ccaacggcaa gacgaccatt acctacatga
     2161 tggaaaccct gctgcgggcc gccggccggc cggcgggggt cctgggcacc atcgaatacc
     2221 gctggcccgg ccacgccgag cccgcggtca acaccacgcc gatggcggcg gacgtccaac
     2281 gcctgctggc gggcatgaag gccgccgggg tcacggatgt cgccatggag gtttcgagtc
     2341 attccctcgc tctgggtcgc gtggaggacg tgtctttctc cgtcggtgtt ttcacgaacc
     2401 tcacgcagga tcatttggat tttcacaaag acatggaaag gtacttcaac gccaaggcgc
     2461 gtttgtttga tctcctggcc cggccggagg gccccgtcgg ccgccgggcg gtattaaacg
     2521 gcgacgatcc ctggacccct cgatttatgg agcgcctcgc cgtgccgcgc tggacgtacg
     2581 ccctggaccg cccggccgac atcggcgcgg cgtccctggc gctgaacgcc gtcggctccc
     2641 ggtttgagtt gcggacgccg gtcggcgcgg cgccggtggc ccttcacctg gtggggcgcc
     2701 acaacgtgta caacgccctc gccgccgcgg gcggcgggtt g
//
LOCUS       DAOQLS010000232         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21869_length_2735_cov_4.872761, whole genome shotgun sequence.
ACCESSION   DAOQLS010000232 DAOQLS010000000
VERSION     DAOQLS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21869_length_2735_cov_4.872761"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /gene="fliD"
                     /locus_tag="PK362_05280"
     CDS             <1..589
                     /gene="fliD"
                     /locus_tag="PK362_05280"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNC74281.1"
                     /translation="LTLRFASPRTLVKAVLRTVDGPGQTAATTGLRDYDLQALRLGGD
                     AAVASDWLTMASVRNNVQGVRANFFTTVTERLRLKILATNAADGRSRVVELEAHESSG
                     AASRTGALIESLTDATDGLFAQAHAKTADQITQIGARIDAANARVDREMTRLETQFRQ
                     MEKALSQLQGMSQSLNALLGVAATSASTNLKASGN"
     gene            592..1011
                     /gene="fliS"
                     /locus_tag="PK362_05285"
     CDS             592..1011
                     /gene="fliS"
                     /locus_tag="PK362_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNC74282.1"
                     /translation="MNSAQKAYRCSDIETASPAKLVLALYDGALSALRRALADIGARR
                     WAEAHRQIVKTQDILGELAGTLDFKSGGEVAPNLFRLYDFMITHLVRANLTKDPALIE
                     QVAALLGPLRDGWEEGVVRSATVAGARPDPLAARGGR"
     gene            1027..>2735
                     /locus_tag="PK362_05290"
     CDS             1027..>2735
                     /locus_tag="PK362_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC74283.1"
                     /translation="MAGTPDAPFRIGLAGYLTGEVSRVRATLVEQGLPGETAVVTEGP
                     GLLEALTRDTLDLLLVDDGPGRATPESVLRQLRETGRNVPVLVWAENVSEVRAVSLLR
                     AGAGDVLSWAQKGRLAPRAQALVHEARNHRRRSRAEALLESTLQRQDLLMRSIPTVSF
                     GARAGDLVRTWVAGHVERLTGYPADKFIGDSEFWYSRLHPDNRESARLARLSLREKGS
                     MRLEYRWMHADGSYRWFLEQAALVRGAKDPGEVVGTWTDVSDRKSLEEQFLQAQKMEP
                     LGRLAGGMVHDLNNLLTVVTGYCEMILADFKEGAPLREELDQINGCARRATQLVGRLL
                     AFSRRQADPPRLVDPNALIQDLQKMLARLIGDDITLVTALEPGVGKIRVDAGQLEQIL
                     LNLAVNARDAMPHGGQLTIETAAVSHIPGPPHSKTKPGPGVLISVRDTGVGMDRETQR
                     RLFEPFFTTKPRGQGTGLGLTTVRALVERSGGHIEIVSHLGLGTGIQLVFPAVWEAAA
                     DAPPAAENIDRGPAGNETILLVEDDESVRDLSHRLLSRLGYNVLVAKDPGEAILIAEN
                     TPRP"
BASE COUNT          451 a          894 c          934 g          456 t
ORIGIN      
        1 cctcacgctc cggttcgcct ccccgcgcac cctggtcaag gccgttcttc gcaccgtgga
       61 cggcccgggc cagaccgccg cgaccacggg gctgcgggac tacgaccttc aggccctgcg
      121 cctggggggc gacgcggccg tggcgtccga ttggctcacg atggcctcgg tccgcaacaa
      181 cgtccagggg gttcgggcga actttttcac caccgtgacg gagcggctcc gcttgaaaat
      241 cctggcgacc aacgccgccg acggccgttc gcgggtggtg gagctggaag cccacgaatc
      301 ctccggcgcc gcctcccgga ccggggcgtt gatcgaaagc ctgaccgatg ccacggacgg
      361 gttgttcgcc caggcccacg ccaaaaccgc ggatcaaatc acccagatcg gggctcggat
      421 cgatgccgcc aacgcccggg tggatcggga gatgacgcgc ttggaaaccc agtttcgcca
      481 gatggaaaaa gccttgtccc agcttcaagg catgagccaa tcgctgaacg ccttgctggg
      541 ggtggccgcg acatcggcct cgacgaacct aaaggcctcg gggaactgat cgtgaacagc
      601 gcccaaaaag cctaccgttg ttcggacatc gaaacggcgt cgccggccaa attggtattg
      661 gccctctacg acggggccct gtcggccctt cgtcgggccc tggcggatat cggcgctcgc
      721 cggtgggccg aggcccaccg ccagatcgtc aaaacccagg acattctggg ggagttggcg
      781 gggacgttgg atttcaaatc cggcggggag gtggccccga acctcttccg tctctacgat
      841 tttatgatca cgcatctggt gcgggccaat ttgacgaagg accccgccct catcgaacag
      901 gtggccgcgc ttttgggtcc gctacgggac ggttgggaag agggggtcgt ccgatcggcg
      961 acggtcgccg gcgcccgccc ggatccgctg gccgcccggg gaggccgctg attcggcggc
     1021 ggcgccatgg cgggaacccc cgacgcgccc tttcggatcg gcctcgccgg ttatttgacc
     1081 ggcgaggttt cgcgcgttcg ggcgaccctg gtggaacagg ggctccccgg ggaaaccgcc
     1141 gttgtgacgg aaggccccgg gctcttggag gccctgaccc gggacaccct cgaccttctg
     1201 ctcgtggacg acgggcccgg tcgggccacg ccggagtctg ttctccggca gctccgcgaa
     1261 accggccgga acgtgcccgt cctggtttgg gccgaaaacg tgtcggaggt tcgggccgtc
     1321 tccctccttc gggcgggcgc gggggacgtg ctgtcctggg cgcaaaaggg ccgtttggcg
     1381 ccccgggccc aggcgttggt ccacgaggcc cgcaatcacc ggcgccgctc ccgggccgag
     1441 gccctgttgg agtccaccct ccagcggcag gaccttttga tgcggtcgat ccccacggtg
     1501 tccttcggcg cgcgggcggg ggatttggtg cgcacctggg tggccgggca cgtcgagcgg
     1561 ttgaccgggt atcccgcgga caaattcatc ggcgacagcg aattttggta ttcgcggctc
     1621 cacccggaca accgggaatc cgcccggctc gcccggttgt cccttcggga aaagggatcc
     1681 atgcgtttgg aataccggtg gatgcacgcc gacgggtcct accggtggtt tttggagcag
     1741 gccgccctgg tgcggggggc caaagacccg ggggaggtgg tcgggacctg gacggacgtg
     1801 tcggaccgga aatccctcga ggagcagttc ctccaggccc aaaaaatgga gcccctcggc
     1861 cggctggccg gcggaatggt ccacgacctg aacaacctgt tgacggtggt gacgggctat
     1921 tgcgaaatga tcctggcgga tttcaaggaa ggggcccccc tgcgggagga actggaccaa
     1981 atcaacggat gcgcccgccg cgccacccaa ttggtcggcc ggctgttggc cttcagtcga
     2041 cggcaggccg accccccgcg cctggtggat cccaacgcgt tgatacagga tttgcaaaaa
     2101 atgttggccc ggcttatcgg ggacgacatc acgctggtga cggcgttgga gcccggggtc
     2161 ggaaaaatcc gggtcgacgc cgggcagttg gagcaaattc tcctcaatct cgcggtcaac
     2221 gcccgggacg ccatgcccca cgggggccag ttgaccatcg agacggccgc cgtttcccac
     2281 atcccgggcc ctccgcattc caaaacaaaa cccggccccg gggttctgat ttcggtccgg
     2341 gacacgggcg tcggcatgga tcgggaaacc caacgccggc tcttcgagcc tttttttacg
     2401 acgaaacccc gggggcaggg gaccgggttg gggttgacca cggtgcgggc cctcgtggag
     2461 cgttccggcg gccacatcga aatcgtcagc cacctggggt tgggaacggg gattcaactg
     2521 gttttccccg cggtgtggga agccgccgcg gacgcgcccc ccgcggccga aaacatcgac
     2581 cggggtccgg cgggcaacga gactattttg ctggtggaag acgacgaatc cgtgcgcgac
     2641 ctttcgcacc ggctgttgtc ccgtttggga tacaacgttc tggtggccaa ggaccccggc
     2701 gaggccattc tcatcgccga aaacaccccc cggcc
//
LOCUS       DAOQLS010000233         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_21984_length_2727_cov_3.526946, whole genome shotgun sequence.
ACCESSION   DAOQLS010000233 DAOQLS010000000
VERSION     DAOQLS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21984_length_2727_cov_3.526946"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2727
                     /locus_tag="PK362_05295"
     CDS             <1..>2727
                     /locus_tag="PK362_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74284.1"
                     /translation="FATDLLHEAYVRHDLAVHSLMRPRPADGRRVFYGGAGADLINVL
                     LSTDGDEIQMAGHYPEVNETVAAGAWSRGPEELFSRTEPYRAFKAKEGWARAVFMTDA
                     EQLMGDVWRELVSLGVDPASVLHRMEGKNWILQFEWRHPAAATSRSRRVVFRDMDVVK
                     SAGDLGWTFDVYYQRAGMEMPKNYAGTPSYLVALSKNLRPDGLFVTDDISDSGFLPGR
                     ADHVRDFPLEGQEVVVPEDSGREAHYLSARRAKFPQVPVPLRMGGLRDVEGYGWRVHV
                     RRFSEFPPRVVDFSSPGSEGGWATPLALIVTAAAALVAAHWGWGDVSQGVALAGGVFL
                     GSLRPAVTDELRVLLDIATDPRRTATPDRWGPGRWATGQPKTHEIQLAFKPTAGPRER
                     TRMAFFKTSVGGRPFSLALAGLRPGIHRVRFSGDDLWPPLLYLTEPDTDRTWAFSLEP
                     FRASRARNGSGSAALLADPYGSYAEAVREAPARVRLARWLEDRRKDRNVPAPAGLAVA
                     LSISGDARSGGALVDPSTGKRLIRLESRAFVPGPAVLEITGEALRVTQGSATVYFELK
                     DVFRFWGGKMERSPRESLAQARLKESDLATRKLLSRGIGELLDDPAGSPSPRAVQRME
                     IFNREAGVLLMDGSGVLSVLLSPETSRRLQRADRAHFTVAGFRPGGAYRPRWMAVAHR
                     GPVLILEDAADPQKLHAFALGGLRQAEGGKKKGGLTPLGVFRTMAQVGDAAAFSPLGL
                     LEEAAARAADWAAGAAPDLEARVFAFNLQKPVAKATLNDGQGLYLESPFTGAVRRVRT
                     QSHQNSLWTLSILLTADGPLLLARAVPSKVREPFKAYGPLVFGDALDRPEVAVWRGDR
                     EASLSSAAESLSRTLDRPVSVPRAGEIGPALSVPSAERAAAHYAEASRRTLAL"
BASE COUNT          391 a          888 c          953 g          495 t
ORIGIN      
        1 cttcgccacg gacttgttgc acgaggccta cgtccgccac gatttggccg tccattccct
       61 catgcggccc cgtcccgccg acggtcgtcg cgtcttttac ggcggcgccg gggccgactt
      121 aatcaacgtc cttctctcca ccgatgggga cgaaatccag atggccgggc attatccgga
      181 ggtgaacgaa acggtcgcgg cgggcgcgtg gtcgcgcggc cccgaggaat tgttctcccg
      241 gacggaacct tatcgggcgt tcaaggccaa agaggggtgg gcccgggccg ttttcatgac
      301 cgacgcggag caactgatgg gggacgtttg gcgggaactc gtttccctgg gagtggatcc
      361 cgcctcggta ctccaccgga tggagggcaa aaactggatc ctccaatttg aatggcgcca
      421 cccggcggcc gcgacgtctc gatcccggcg agtcgtcttc cgggatatgg acgtggtcaa
      481 atccgccggg gacctcggct ggacgttcga cgtttactat cagcgcgccg gaatggagat
      541 gccgaagaac tacgcgggga ccccgagcta tctggtcgcc ctgtcaaaaa atcttcgccc
      601 cgacggcctc ttcgtcaccg acgacatttc ggattccggc tttctccccg ggcgggccga
      661 ccacgtccgg gattttccgc tggaaggcca ggaagtggtc gtgccggagg attcgggacg
      721 ggaagcccat tatctttccg ctcggcgggc gaagttccct caagtgccgg tgccccttcg
      781 gatgggggga ttgcgggacg tggaaggtta cggatggcgg gtccacgtgc gtcgtttttc
      841 ggaatttcct ccccgggttg tggatttttc atcgccgggt tccgaggggg gctgggcgac
      901 gccgctggcc ctgatcgtga cggccgcggc ggccttggtc gcggcccatt gggggtgggg
      961 agacgtctcc cagggggtcg ccctggcggg cggcgtcttt ttgggttccc ttcggcccgc
     1021 ggtcaccgat gaattgcgcg tgttgctgga catcgccacc gatccgcgcc gaaccgcaac
     1081 gccggaccgt tgggggccgg ggcgatgggc gacgggtcaa cccaagaccc acgagattca
     1141 attggcgttc aagcccaccg ccgggccccg ggaacgcacg cgaatggcct tcttcaagac
     1201 gagcgtcggg gggcggccct tttccctcgc tctggcgggt ctccggccgg ggattcatcg
     1261 ggtccgtttt tccggagacg acctttggcc gccgctcctt tacctgacgg aacccgacac
     1321 cgaccgcacc tgggccttct cgttggagcc ttttcgcgcc agccgggccc ggaatgggag
     1381 cggctccgcg gccctgctgg ccgatcccta cgggagctac gccgaggcgg tccgggaagc
     1441 ccccgcgcgg gtccgcctgg cgcgatggct ggaggatcga cggaaggacc ggaacgttcc
     1501 ggcgcccgcc ggactcgccg tggccctgtc gatcagcggg gacgcccgaa gcggcggcgc
     1561 tctggtggat ccgtccacgg gcaagcggct gatccggttg gaaagccgcg cttttgttcc
     1621 gggtcccgcg gttctggaaa tcacgggcga ggcgttgcgg gtcacccagg ggtccgccac
     1681 ggtctatttc gaattgaagg acgtgttccg attttggggc ggcaaaatgg aacggagccc
     1741 ccgggaatcc ctcgcccagg cgcggttgaa agagagcgac ttggcgacgc gcaaattgtt
     1801 gtcccggggg atcggcgaat tgttggacga cccggccggt tccccgtcgc cccgggccgt
     1861 tcagcgcatg gaaatcttta accgggaggc cggcgtcctg ttgatggacg gatcgggcgt
     1921 gttgagcgtt ctgttgtccc cggaaacgtc ccggcgcctc caacgggcgg accgggcgca
     1981 tttcaccgtg gcgggctttc gccccggggg cgcctaccgt ccgcggtgga tggccgtggc
     2041 gcaccggggc cccgtgctca ttctggagga cgccgccgac ccgcaaaaac ttcacgcgtt
     2101 cgccctcggc ggccttcgcc aagccgaagg gggcaagaaa aagggcgggt taacgcccct
     2161 gggcgttttt cgaacgatgg cccaggtggg ggacgccgcc gccttctccc cgctgggcct
     2221 cctggaggag gccgcggccc gggccgccga ttgggccgcc ggcgcggcgc ccgatttgga
     2281 agcccgggtg ttcgctttca atcttcaaaa accggtggcc aaggcgacgc tcaacgacgg
     2341 gcagggcctt tatttggaaa gccccttcac cggggcggtg cgccgggttc gaacccagtc
     2401 ccaccaaaac agcctttgga ccctttccat tctgttgacg gccgacggcc ctcttttgct
     2461 ggcccgggcg gtgccctcca aggtgcgaga acccttcaaa gcctacggcc ccctggtttt
     2521 tggcgacgcc ctggaccgtc ccgaggtcgc ggtctggcgc ggcgatcggg aagcgagttt
     2581 gtcgtcggcg gcggagtcgc tgagccggac gctggaccgg cccgtgtcgg tgccccgggc
     2641 gggggagatc ggccccgccc tgtcggtccc ttcggccgag cgggcggcgg cccactacgc
     2701 cgaggcctcc cggcgcacgc tggccct
//
LOCUS       DAOQLS010000234         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22043_length_2722_cov_5.137233, whole genome shotgun sequence.
ACCESSION   DAOQLS010000234 DAOQLS010000000
VERSION     DAOQLS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22043_length_2722_cov_5.137233"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PK362_05300"
     CDS             complement(<1..677)
                     /locus_tag="PK362_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNC74285.1"
                     /translation="MKRLSLTLLSLVGLTGLAFAAKLTNRPIATVNGEAILLSEYEKN
                     WASLLEQAKESMPADKLDDAWKKTNRDRLLDQMIADRVLLQEAKKRKLRVNQREFENG
                     VAQVRARFLPEKAQKELQGILRRQYAALGAEANESALDFPAALKELAASNPGAVKEAD
                     ANFAAELAKEGIDQKKFEDRIRDQLSVMELSKEAVRARTKPPTDEDAKKLFDRIQLKL
                     QGKPVPG"
     gene            complement(737..1129)
                     /locus_tag="PK362_05305"
     CDS             complement(737..1129)
                     /locus_tag="PK362_05305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC74286.1"
                     /translation="MRKKIAAGEKFEDLARARSEDPGTGQKGGDLGYLIRGSLVPEFE
                     NALFQMKTGEVSDPVASPYGFHLIERTGDRKLSAAPLDDAAKKRIRAALESQKFQQWF
                     DRTRAQYAVSVNDAALDDLAVSTAPGRP"
     gene            complement(1126..1614)
                     /locus_tag="PK362_05310"
     CDS             complement(1126..1614)
                     /locus_tag="PK362_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74287.1"
                     /translation="MRKPPLSLAAAALLCGAACQKKDPVLARVGTQVVRASSFQDEIN
                     GVPFSSRAYLGSPAGRRELLDLLIRRKIILQAAQDNPAPGPALRERLAELELNYRQSR
                     AHLRQKYLEERERLWVSHFMDGLKKNRLSATDDDVRRFWDTESEVRAATSWSATGPWP
                     RN"
     gene            complement(1624..>2722)
                     /locus_tag="PK362_05315"
     CDS             complement(1624..>2722)
                     /locus_tag="PK362_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNC74288.1"
                     /translation="GPFNREVVIPAVEQELRRGGQVYYVHNRVGSIEKRKEWLEKTLA
                     EHGLPAKIVIAHGQMTGPALEKVMWDFLHRKFDILLATSIIESGLDIPSVNTLIVEEA
                     EDFGLAQLYQLRGRVGRERQRAYCYLFYSSDAALTDDAQKRLSALKEFASLGAGFRLA
                     MRDLEIRGAGNLLGAEQSGHLNAIGVDLYGQLLSEEIERQKQGGGPAGDGTVETALEL
                     PVSAYLSAEVLPSEADRVVFYKRLLDAGPGDLEGLRAELEDRCGRLPPEAERLFAVTR
                     LRQTAQAKKVVHVGLVEDGLEFRFREGTALAGKDLARLMAAHGDRFRFLPPTKDTPPG
                     MRFEPPLPPDLVDWSTAFLDQLSLDTAGQKS"
BASE COUNT          455 a          838 c          875 g          554 t
ORIGIN      
        1 aatcccggca cgggtttgcc ctgaagcttc agttggattc ggtcgaagag ctttttggcg
       61 tcctcgtccg tcggcggctt ggttcgggcc cgaacggcct ccttggacag ctccatgacg
      121 gacagctgat cccggatacg gtcctcgaat tttttttggt cgatgccttc cttggccaat
      181 tccgcggcga aattggcgtc ggcctctttg acggccccgg ggttgctcgc ggccagctct
      241 ttcagggcgg cggggaaatc cagggcgctt tcgttggcct cggcgcccag ggccgcgtat
      301 tgccgacgca aaatcccctg gagctccttt tgggcctttt ccggcagaaa tcgggcccga
      361 acctgggcga cgccgttttc gaattcccgt tggttgaccc ggagttttct tttcttcgct
      421 tcctggagca ggacgcggtc ggcgatcatt tgatccagaa gccggtcccg gttggttttt
      481 ttccaggcgt cgtcgagctt gtcggccggc atggattcct tggcctgttc gaggagggac
      541 gcccagtttt tctcgtattc ggacaacaaa atggcttcgc cgttgaccgt ggcgatgggg
      601 cggttcgtga gtttggccgc gaaggccaga ccggtcaggc cgaccagcga caatagggtg
      661 agggacaaac gtttcaaggg agaacctcct cgggagtgat gccgcaacta tatcattttg
      721 attcgacgac ccggatttac ggccgtccgg gcgccgtgga caccgccaag tcgtccaagg
      781 cggcgtcgtt gacggacacc gcgtactggg cccgggtccg atcgaaccat tgctgaaatt
      841 tttggctttc cagggcggcg cggattcgct tcttggcggc gtcgtccagg ggagcggcgg
      901 acaattttcg atcgccggtc cgttcgatca aatggaaacc gtagggggag gccacggggt
      961 cggaaacctc gcccgttttc atttgaaaga gcgcgttttc aaattcggga accagggagc
     1021 cccggatcaa ataccccagg tccccgccct tttggccggt gcccggatct tcggaccggg
     1081 cgcgggcgag atcctcgaat ttttcccccg ccgcgatttt cttcctcaat tccgcggcca
     1141 gggcccggtc gctgaccagg atgtggcggc gcgcacttcg ctttccgtgt cccaaaagcg
     1201 gcggacgtcg tcgtcggtcg cggacaatcg gtttttcttc aaaccgtcca tgaaatggga
     1261 cacccagagg cgttcccgct cctccaaata tttctgacga aggtgggccc ggctttggcg
     1321 gtagttgagt tccagttcgg ccaatcgttc ccgaagggcc ggtcccgggg cggggttgtc
     1381 ctgggccgct tggaggatga tcttccggcg gatcagaagg tccaagagct cgcgccgccc
     1441 ggcgggggag cccaaatagg ctcggctgga aaaggggacg ccgttgattt cgtcttgaaa
     1501 agaggacgcc cggaccacct gggtcccgac ccgggccagg accggatctt ttttttggca
     1561 ggccgccccg cacagaaggg ccgcggcggc gagggaaaga gggggctttc gcattccggc
     1621 aaactaactt ttttgccccg cggtgtccag ggaaagttgg tccagaaaag ccgtggacca
     1681 atccaccaaa tcgggcggaa ggggcggctc gaatcgcatg cccggcgggg tgtctttggt
     1741 ggggggtaaa aagcggaacc ggtccccgtg ggcggccatc aggcgggcca agtccttgcc
     1801 cgccagggcg gtgccctctc taaaccgaaa ttccaaaccg tcttccacca ggccgacgtg
     1861 caccaccttt ttggcctggg cggtttgccg caggcgcgtg acggcgaaga ggcgctcggc
     1921 ctcggggggc aatcggccgc agcgatcctc gagttcggcc cgaaggccct ccaggtcccc
     1981 cggaccggcg tccaacaggc gcttgtagaa caccacccgg tcggcctccg acggcaggac
     2041 ctccgccgag aggtaggccg acacgggcaa ctccagggcc gtttccacgg tcccgtcccc
     2101 ggcggggccg ccgccttgtt tttgacgttc gatttcctcg gagagcaact gcccgtagag
     2161 gtcgacgccg atggcgttca aatggccgct ctgctcggcg ccgaggaggt tcccggcccc
     2221 gcgaatttcc aaatcccgca tggccaggcg gaaacccgcc cccagggagg cgaattcctt
     2281 cagggccgac aggcgctttt gggcgtcgtc ggttaaagcg gcgtcggagg aatagaacag
     2341 gtagcagtag gcccgctggc gttcccggcc cacccggccc cgcagttggt agagctgggc
     2401 caggccgaaa tcctcggcct cttcgacgat cagcgtgttg accgacggga tgtccaggcc
     2461 cgactcgatg atgctcgtgg ccaacaaaat gtcgaatttc cggtgcagga aatcccacat
     2521 gaccttttcg agagccgggc cggtcatttg cccgtgggcg atgacgatct tggcgggaag
     2581 cccatgctcg gccagggttt tctccagcca ttcctttcgt ttttcgatgg accccacccg
     2641 gttgtgcacg taatacacct gcccgcctcg gcgcagttcc tgctcgaccg cgggaatcac
     2701 gacctcccgg ttgaagggcc cc
//
LOCUS       DAOQLS010000235         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22145_length_2714_cov_4.895825, whole genome shotgun sequence.
ACCESSION   DAOQLS010000235 DAOQLS010000000
VERSION     DAOQLS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22145_length_2714_cov_4.895825"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK362_05320"
     CDS             complement(<1..427)
                     /locus_tag="PK362_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC74289.1"
                     /translation="MSIQQLLQVVHKEGASDLHLKVGRPPLIRLQGEITPVAGEAPLT
                     MDEVLNHVYSVINTAQRTLLERDRELDFSFYLRGIARYRGNAFYQKGAIGAVFRMIPA
                     QIPTIESLGLAPVLKEIVSRRQGFFLVTGPTGSGKTTTLA"
     gene            complement(529..1623)
                     /locus_tag="PK362_05325"
     CDS             complement(529..1623)
                     /locus_tag="PK362_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNC74290.1"
                     /translation="MIDILRLAVKQGASDVHLLIGRPPMTRVHGEVLDMPGLPALTSE
                     EAKRLIYSMLYDEQKQKFEQNLELDCSFSIAGLARFRVNVLMQINGIEAVLRVIASKV
                     PAPDEIKLSPAILEFANLSRGLVLVTGATGAGKSTTLAALIEIINQVRRQHILTVEDP
                     IEFVYEPKRCIVRQREVGHSTHSFQEALKHALRQDPDVILIGEMRDIETISMALTAAE
                     TGHLCFATLHTTDAAQTVDRIIDVFPPHQQQQVRVQLSTVLKGVVCQTLVPVTDGHGR
                     VAAREVMVVTPAIANLIREGKTHMIYGAIETGSKYGMVPLDRALADLVNSGMISFEDA
                     LAKASDPQKLREYSGHGSGPRVESNRSVVY"
     gene            complement(1660..>2714)
                     /locus_tag="PK362_05330"
     CDS             complement(1660..>2714)
                     /locus_tag="PK362_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNC74291.1"
                     /translation="YDPRQTKLNVLSIPRDTKIDLPGYRFRRINEVFAYHYAAQRNVP
                     AAVHPVMDAVAQLLPSGGALQPRYFFQVDYQGFRRMIDRLGGVTVSVDEPMHYDDHAG
                     NFHFHKDPGVYHLDGLDALGYVRYRGKSGDRGRILRQMEFLRSLLRLATSPTVFWRWP
                     RALSEAAWALHTNVGALELPFLFAEGKKMRGPDINPWLLPGRPRGAYWEMDKDRAAYV
                     VQQILKGESAGAADAVSAEDSSREAEGPEATGGPAPEAGRAPTVKVWNASGRAGLALE
                     VTRRLRRDGFDVIDWGNYTGRQNKSRVVDRSGHFERARRVADRLGLLGAYSDADPALR
                     TDVEVVLGEDFAGTER"
BASE COUNT          499 a          868 c          844 g          503 t
ORIGIN      
        1 cggccagcgt cgtcgtcttg cccgagcccg tggggcccgt cacgagaaag aacccctgcc
       61 ggcgggaaac gatttccttg agcaccgggg cgagccccag ggactcgatg gtcgggatct
      121 gcgccgggat catccggaac acggcgccga tggcgccctt ttggtaaaaa gcgtttccgc
      181 ggtaacgggc gatgcctcga aggtaaaacg agaagtccaa ttcccggtcc cgctccagca
      241 gggtccgttg ggccgtgttg atcacggaat aaacgtggtt caacacctcg tccatggtga
      301 ggggggcttc ccccgccacc ggcgtgattt cgccctgaag ccggatgagg ggcggacggc
      361 ccactttcaa atgaaggtcg ctggcgcctt ccttgtgaac cacctgcaat agctgttgaa
      421 tgctcatgcc catgaagtcc tcctcaatgc atcgccctca aacccttgca acaaacgcgc
      481 caccggcccg gtaagtatcg ccccgggaca tcggaaaatc aatgcaaatc agtagacaac
      541 ggaacggttg ctttccaccc gcggacccga accgtggccg ctgtattccc gcagtttttg
      601 ggggtccgag gccttggcca gggcgtcctc aaaggaaatc atgccggaat tgaccaggtc
      661 cgccagggcc cgatccaggg gcaccatgcc gtatttggaa ccggtttcga tggctccgta
      721 gatcatgtgg gttttcccct cccggatcag gttggcgatg gcgggggtca ccaccatcac
      781 ttcccgggcg gccacgcggc cgtggccgtc ggtcacgggc acgagggttt ggcagaccac
      841 gcctttcaac accgtcgaca gttgcacgcg cacctgttgt tgctggtggg gcgggaagac
      901 gtcgatgatg cggtcaacgg tttgggcggc gtcggtcgtg tgcagggtcg cgaagcacag
      961 gtggcccgtt tcggccgcgg tcagcgccat ggaaatcgtc tcgatgtccc gcatttcgcc
     1021 gatcaagatg acgtcggggt cctggcggag ggcgtgtttc agggcctcct ggaaactgtg
     1081 ggtggaatgc ccgacctccc gctgccggac gatgcaccgt ttcggttcgt agacgaattc
     1141 gatggggtct tcgacggtca aaatgtgctg gcggcggacc tgattgatga tttcaatcaa
     1201 ggcggccagg gtggtcgatt tgccggcgcc cgtggcgccc gtcaccagga ccaggccccg
     1261 ggacaggttg gcgaattcca aaatcgcggg ggacaatttg atttcgtcgg gcgcgggcac
     1321 cttggaggcg atcacccgca agacggcctc gatcccgttg atctgcatga gcacgttgac
     1381 gcgaaagcgg gccaacccgg cgatggagaa agagcagtcg agttcgaggt tttgctcgaa
     1441 tttctgcttt tgctcgtcgt agagcatgga ataaatcagt cgtttcgcct cttccgaggt
     1501 caaggcgggc agtcccggca tgtccaggac ctcgccgtgc acccgggtca tgggcggacg
     1561 gccgatcagc aaatgcacgt cggacgctcc ctgcttgacg gccaatcgca ggatatcgat
     1621 catctccatc ggtcaacctc ccggttgttg aaacgcgtgt tatcgttcgg tgcccgcaaa
     1681 atcttcgccc aacaccacct cgacgtcggt ccgaagggcc gggtcggcgt ccgaataagc
     1741 gcccaacaaa cccaaccgat ccgccacccg gcgcgcccgc tcaaagtgcc ccgaacggtc
     1801 caccacccgg cttttgttct gccgcccggt gtaattgccc cagtcgatga cgtcgaatcc
     1861 gtcccggcgg agtcgtcgcg tgacctccag ggccaaaccc gcccgtcccg acgcgttcca
     1921 caccttgacg gtgggagccc ggcccgcttc cggcgccggc cccccggtcg cttccggacc
     1981 ctcggcttcc cttgaagaat cctccgcgct gacggcgtcc gcggcccccg ccgactcgcc
     2041 tttgagaatc tgctgaacca cgtaggccgc ccggtccttg tccatctccc agtaggcgcc
     2101 ccgggggcgg cccggaagca accagggatt gatgtccggc ccccgcattt tctttccctc
     2161 ggcgaagagg aacggcagtt ccagggcccc cacgttcgtg tgcagggccc aggccgcttc
     2221 ggagagggcc cggggccacc gccaaaagac ggtgggcgac gtggccaaac gcaataacga
     2281 ccggagaaat tccatctggc gcaaaatgcg gccccggtcg ccgcttttgc cccggtaccg
     2341 gacgtagccc agggcgtcga gaccgtccaa atggtaaacg cccgggtcct tgtgaaagtg
     2401 gaaattcccc gcgtgatcgt cgtagtgcat gggctcgtcg acgctgaccg tgacgccccc
     2461 caaccggtcg atcatccggc ggaagccctg gtagtccact tgaaaaaaat accgcggctg
     2521 gagcgcgccg cccgaaggca acaactgggc caccgcgtcc atcacggggt gcaccgcggc
     2581 cgggacgttc cgctgggccg cgtagtgata agcgaacacc tcatttatac gccgaaaacg
     2641 gtatcccggc aagtctattt ttgtgtcccg gggaatggac aggacgttca acttggtttg
     2701 gcggggatcg tacc
//
LOCUS       DAOQLS010000236         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22150_length_2714_cov_3.977811, whole genome shotgun sequence.
ACCESSION   DAOQLS010000236 DAOQLS010000000
VERSION     DAOQLS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22150_length_2714_cov_3.977811"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PK362_05335"
     CDS             complement(<1..813)
                     /locus_tag="PK362_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNC74292.1"
                     /translation="MCGIVGKIAWSGAVDIATLERMNDRLAPRGPDEKGLWVSGPIGL
                     AMRRLKVIDLVGGQQPMSNAHCPAAEKAGRFHLVFNGEIYNHRALRGELIRRGHRFAS
                     TSDTEVLLHLFEEEGDRGWERLRGMFAVALFDERRNVLRLARDRMGIKPLYYARTLAG
                     FSFASEVKALLADGDTPREVNAAALNAYFSLGYVPTPVSAFEHVQKLAPGRVLTLELK
                     TGTVTQAPYYEFFPRPPQPRPLSETLETLDQLLTETVRDHLVSDVPLGLFLSG"
     gene            complement(819..1778)
                     /locus_tag="PK362_05340"
     CDS             complement(819..1778)
                     /locus_tag="PK362_05340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC74293.1"
                     /translation="MNPRLSVVVPVLNESKNLPALMDGLSRALHGLPAEFLFIDDGST
                     DDGASLIEQRRAQDPRVHLVSLARRFGQTAALSAGLEKARGDVIVTLDADGQNDPADI
                     PALLALLDQGADVVCGWRRTRHDPWLSRRLPSGIANRIISWVTGVALHDYGCTLKAFR
                     RSAVEGLRLYGEMHRLIPVWCAWRGAVIREIEVRHHPRVHGRSHYGIGRTFKVLLDLL
                     TAKFFFSFLTSPSHPLGGLGLVFLFLGFLSGLFPILDKFVFNHWALYRIPFMILSVFL
                     GLLGMQFLALGVLAEILVRIYYENRGEKPYRVKRVVPDDAAPR"
     gene            1885..2661
                     /locus_tag="PK362_05345"
     CDS             1885..2661
                     /locus_tag="PK362_05345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC74294.1"
                     /translation="MTADPRSLKEINRAHYDALHPGQRDYWRWMAAPRARVRAVLAAL
                     AESPPSALVDLGCGDGTLLRTIGRRFPGLVRAGVDLAAAQIEENRRNDPAVEWRAFDL
                     DASTPASGPGRGAFDAVVACEVIEHVESPRRFLENARALAAPGGRLVLTTQSGRVHAT
                     ERRVGHRRHFTVQEMTELLVAAGWRPERVWNEGFPFHDASKALANLWPSAVLKAFGEK
                     PYTLFQKSVCAVLAVLFAFNSRRRGAQLYALARNPPAPGA"
BASE COUNT          455 a          879 c          954 g          426 t
ORIGIN      
        1 gccggagagg aacagcccca ggggaacgtc cgacaccaaa tggtcccgca cggtttcggt
       61 cagcagctgg tccagggtct cgagggtctc cgacagcggt cggggctggg gcgggcgggg
      121 gaaaaattcg tagtagggcg cctgggtcac cgtcccggtt ttgagttcca gcgtcaggac
      181 cctccccggc gccagttttt ggacgtgttc aaaagcggac accggggtgg ggacgtaccc
      241 gagggaaaag taggcgttca gggcggcggc gttcacctcc cggggcgtgt cgccgtcggc
      301 caggagggct ttgacttcgg aggcgaagga aaacccggcg agggtccggg cgtaatacag
      361 cggcttgatg cccatgcggt cccgcgccag gcgcaggacg ttccgccgtt cgtcgaagag
      421 ggccacggcg aacatgcccc gaagtcgttc ccacccccgg tcgccctcct cctcgaagag
      481 atggaggagg acctcggtgt ccgaggtgga ggcgaaccgg tgcccccgtc ggatcagttc
      541 gccgcggagg gcgcggtggt tgtagatttc cccgttaaaa acgaggtgaa agcgtcccgc
      601 tttttccgcc gcggggcagt gggcgttgga catgggctgc tggccgccca ccaaatcgat
      661 gaccttgagt cggcgcatgg ccaatccgat ggggccgctg acccagaggc ccttttcgtc
      721 cgggccccgg ggggccaggc ggtcgttcat ccgctcgagg gtggcgatgt ccacggcccc
      781 ggaccaggcg attttgccga cgatgccgca catggcgttt agcggggggc ggcgtcgtcg
      841 gggacgacgc gcttgacgcg gtagggcttt tccccccggt tctcgtaata aatgcggacc
      901 agaatttccg ccagtacgcc cagggcgagg aattgcatgc ccaggaggcc caggaacacg
      961 gacagaatca tgaaggggat ccggtagagg gcccagtggt tgaaaacgaa tttgtccagg
     1021 atggggaaaa ggcccgagag aaagccgaga aagagaaaca ccaagcccag gccgcccagg
     1081 gggtggctgg ggctggtgag aaaactgaaa aagaatttgg ccgtcagaag gtccagcaac
     1141 accttgaagg tccgtccgat cccgtagtgc gaacggccgt ggacccgcgg gtgatgccgg
     1201 acttcgattt cgcggatcac cgccccccgc caggcgcacc acaccggaat caaccggtgc
     1261 atttcgccgt acaagcgcag gccctccacg gcggaccgcc ggaaggcttt gagcgtgcag
     1321 ccgtaatcgt gcagggccac gcccgtcacc caggagatga tgcggttcgc gatgcccgag
     1381 ggcaggcgtc gcgagagcca gggatcgtgg cgggttcggc gccagccgca gaccacgtcc
     1441 gcgccctggt ccaaaagggc caacagcgcc ggaatgtccg cgggatcgtt ttgtccatcg
     1501 gcgtccagcg tgacgatgac gtcgccccgg gccttttcca gtccggccga gagcgccgcc
     1561 gtttgcccga accgccgggc cagagacacc aggtgcaccc gggggtcctg ggcgcggcgt
     1621 tgttcgatga gcgacgcgcc gtcgtccgtg gatccgtcgt cgataaaaag gaattcggcc
     1681 gggaggccgt gcagggcccg actcagcccg tccatcaggg ccggcaggtt tttggactcg
     1741 ttcaacaccg gaacaacgac gcttaagcgc ggattcatgg gtttgattga agcgccatta
     1801 tagcaccggc ggcggggaat tccgccgcgc cggcggtttg acggcgcggt cgcagggagt
     1861 acaatggccg gccaggaccg ttccgtgacc gccgaccccc gctccctcaa ggaaatcaac
     1921 cgggcgcact acgacgccct ccatcccggc cagcgcgatt actggcgctg gatggcggcg
     1981 ccccgcgccc gggtgcgcgc cgtgctggcg gccctggcgg agtcgccccc gtcggcgttg
     2041 gtggatctgg ggtgcggcga cggcaccctc cttcgaacga tcggccggcg gtttcccgga
     2101 ttggtccggg ccggcgtgga cctcgcggcg gcccagatcg aggaaaaccg gcggaacgac
     2161 cccgccgtgg aatggcgggc cttcgatttg gacgcttcga ccccggcgtc ggggcccggg
     2221 cggggggcgt tcgacgccgt cgtggcctgc gaggtcatcg agcacgtgga gtccccccga
     2281 cgatttttgg aaaacgcccg ggccctggcc gcgcccggag ggcgcctggt gctcaccacc
     2341 caaagcggcc gggtccacgc caccgaacgc cgggtgggcc accgccggca tttcaccgtc
     2401 caggaaatga cggaattgct cgtcgccgcg ggctggcgtc ccgagcgtgt gtggaacgag
     2461 gggtttcctt ttcacgacgc ttcgaaggcc ctcgccaacc tgtggccgtc ggcggtgctc
     2521 aaagccttcg gggaaaaacc ctacacgctg ttccaaaaaa gcgtgtgcgc ggtgctggcc
     2581 gtcctgttcg ccttcaattc gcggcgtcgc ggggcccaac tctacgccct ggcccgcaac
     2641 cccccggcgc ccggcgcgtg acggtccgac ccgcgccgtc gccgcgcttc gcgcccgccg
     2701 cggccctcct gttg
//
LOCUS       DAOQLS010000237         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22250_length_2707_cov_4.694193, whole genome shotgun sequence.
ACCESSION   DAOQLS010000237 DAOQLS010000000
VERSION     DAOQLS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22250_length_2707_cov_4.694193"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PK362_05350"
     CDS             complement(<1..185)
                     /locus_tag="PK362_05350"
                     /inference="COORDINATES: protein motif:HMM:NF023393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HNC74295.1"
                     /translation="MYHNVAAVVLGAEPASETDKRLTLFTREKGRLFARAAGARRPGA
                     KLAAATEPASLSRFRLWL"
     gene            complement(187..1572)
                     /gene="mgtE"
                     /locus_tag="PK362_05355"
     CDS             complement(187..1572)
                     /gene="mgtE"
                     /locus_tag="PK362_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNC74296.1"
                     /translation="MSSLQPSALFIPEIKELLRRKEYRELKADLTEINPADLAEGWAR
                     LSPFERIVTFKLLPIVRACELFEELDADQQIQLLSALEFNALGPVLEELGPEVAGALF
                     HRLPERSLRRMIALVRRAQTTRPERERVFPPNTAGALMHTDSLTLGPKLTAQQALDLV
                     RSVSRLHRMSDLNVIYVVDDQGRLMGVVTPRALIAAPRDMRMSQLMGPVQLIKVRADM
                     DQEAAAQVFAKYKLMAAPVVDQDNRLLGVLTVDDILHVISQEATEDIAKMAGTTAEEL
                     EEASPGRIARLRMPWLITTCLAEVVVSAIVRGFETTLAQAVALASFMPLIAAMGGNVG
                     TQSSTLCVRGLATGHVQPGDWKKMARREFLAGLYMGAGYGLAVAAVAFVTYHGRFGFA
                     FPVVVFTGVLFSMTIASTMGAIEPFILHSFKMDPAVAVGPLVSTMTDLMSVTAYLTLA
                     TIALSAGWLVR"
     gene            complement(1569..2585)
                     /locus_tag="PK362_05360"
     CDS             complement(1569..2585)
                     /locus_tag="PK362_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNC74297.1"
                     /translation="MTVLGVKLLLGLFLTVLSAGFSLAETSFMSLSRQQLTRLAVAHP
                     GRLDFWRRDPDRALAVLLFLNNFVNAGLGVLSVSMALAAAEGLGVPFAWGGVVFPAAA
                     GLFLVVFGEILPKVVARGQAERLALALAPTLEIFSDVFGPWMEGLLKRTGGLLSWLSR
                     TVRKERGQWDAAVIRALLDGAPAAHPVRELLNNVIGFGAVPVKAVMVPREEITAADLS
                     AGKTALIERVLASGYSRVPVFRGSLDSVEGMVYAKDFLAEWRSESLIALDDLVRPVLR
                     VPESTPLAQLLRVFRQGHHHLALVIGADGRLSGLVTLQDTLEAIVGEIAKERSTKPSA
                     PPPS"
BASE COUNT          519 a          894 c          892 g          402 t
ORIGIN      
        1 agccacaggc gaaagcggga caacgaggcc ggttccgtgg ccgcagccaa tttggctccg
       61 gggcgacgcg ccccggcggc ccgggcgaag aggcggccct tctcccgggt gaacaacgtc
      121 agccgcttgt cggtttcgga agccggttcg gccccgagga cgacggcggc gacgttgtga
      181 tacacgtcaa cgcaccagcc agcccgccga cagggcgatg gtggccaggg tcaggtaggc
      241 cgtgacgctc atcaaatccg tcatggtgct cacgaggggc cccacggcca ccgccgggtc
      301 cattttgaaa ctgtgaagga tgaagggctc gatggccccc atggtcgaag cgatggtcat
      361 cgaaaaaaga acgccggtaa aaaccaccac gggaaaggcg aaaccaaacc ggccgtggta
      421 ggtgacgaag gccacggccg ccacggccag tccgtacccc gcgcccatgt agagcccggc
      481 caaaaattcc cgccgggcca tttttttcca gtcgccgggt tgcacgtggc ccgtggccaa
      541 cccgcgcacg cacagggtcg aggactgcgt gcccacgttc ccccccatgg ccgcgatgag
      601 cggcatgaaa ctggccaggg ccacggcctg ggccagggtt gtttcgaagc cccggacgat
      661 ggcgctcacc accacctcgg ccaaacaggt cgtgatgagc cagggcatgc ggaggcgggc
      721 gatgcggccc gggctcgcct cctccaattc ctcggccgtg gtgccggcca ttttggcgat
      781 gtcctcggtg gcctcctggc tgatgacgtg gaggatgtcg tccaccgtga gaacgcccaa
      841 cagccgattg tcctggtcca cgacgggggc ggccatcaat ttgtatttcg cgaagacctg
      901 ggcggccgct tcctggtcca tgtccgcgcg gaccttgatc agctgaaccg ggcccatgag
      961 ctgggacatg cgcatgtccc ggggcgcggc gatgagcgcc cggggcgtca ccacgcccat
     1021 gaggcgtccc tggtcgtcca ccacgtaaat cacgttcaag tcgctcatgc ggtggaggcg
     1081 gctcaccgag cgcaccaggt ccaacgcttg ctgggcggtc aatttggggc ccaaggtcaa
     1141 ggaatcggtg tgcatcaggg cgccggccgt gttcggcggg aaaacgcgtt cccgctccgg
     1201 ccgggtcgtc tgggcccgac ggaccaaagc gatcatgcgc cgcagggacc gctccggcaa
     1261 ccggtggaaa agggcccccg cgacctcggg gcccaattcc tccagcaccg gccccagggc
     1321 gttgaactcc agggccgaca gcagttggat ctgttggtcg gcgtccaact cctcgaagag
     1381 ctcgcaggcc cgcacgatgg gcagaagctt gaaggtcacg atccgctcga agggcgacag
     1441 ccgggcccac ccttcggcca ggtccgcggg gttgatttcc gttaaatcgg ctttgagctc
     1501 gcgatattcc ttgcggcgca aaagttcctt gatttcgggg atgaagaggg cggaaggttg
     1561 gagggaactc atgacggggg cggagcgctg ggcttggtcg accgttcctt ggcgatttcg
     1621 ccgacgatgg cttcgagcgt gtcctgaagg gtgaccaggc ccgagagccg tccgtcggcc
     1681 ccgatgacga gggccaggtg gtggtggccc tgccgaaaaa cgcgcagcaa ctgggccagg
     1741 ggcgtggact ccggcacgcg caacacgggc cgcaccaaat cgtccaacgc gatcagggat
     1801 tcgcttcgcc actcggcgag gaagtcctta gcgtaaacca ttccctccac gctgtcaagc
     1861 gaaccgcgga acacggggac ccgggaatat ccgctggcca aaacgcgctc gatcagggcc
     1921 gttttcccag cggaaaggtc cgcggccgtg atttcctccc ggggcaccat gacggccttg
     1981 accggcaccg cgccgaaccc gatcacgttg tttaaaagtt cccggaccgg gtgcgcggcg
     2041 ggggcgccgt cgagcagggc gcggatcacg gccgcgtccc actggccccg ttctttgcgg
     2101 accgtgcgcg acaaccagga caaaagaccg ccggtgcgct ttaaaagccc ctccatccac
     2161 ggaccgaaaa catcggagaa aatttccaag gtcggggcca gggccaaagc cagtcgttcg
     2221 gcctgccccc gggccacgac cttgggcagg atttcgccga acaccaccag gaacaatccg
     2281 gccgccgccg gaaacacgac gccgccccag gcaaacggga cgcccaaccc ctcggccgcc
     2341 gccagggcca tggacaccga aaggaccccc agccccgcgt tgacgaaatt gttcagaaag
     2401 agcagaacgg ccagggcccg gtcggggtcc cggcgccaga aatccaaccg gccgggatgg
     2461 gccaccgcca ggcgggtcaa ttgctggcgg gacaggctca taaaggaggt ttcggccagg
     2521 gagaacccgg ccgacagcac cgtcaggaac aggcccagga gaagtttgac gccgagaacg
     2581 gtcacggggt ccccccttgc tcctccaggt cgaactcgtc caggatgtct ccgacgattt
     2641 cctccagaat gtcctccaaa gtgaccaggc cctccggcgg ttcgcccggc gactgaacca
     2701 gggcgat
//
LOCUS       DAOQLS010000238         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22448_length_2695_cov_3.817424, whole genome shotgun sequence.
ACCESSION   DAOQLS010000238 DAOQLS010000000
VERSION     DAOQLS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22448_length_2695_cov_3.817424"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="PK362_05365"
     CDS             <1..1521
                     /locus_tag="PK362_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNC74298.1"
                     /translation="STVATTGDGLAELKRALEHPTCSNVAPPDNPDIARALAALAPHF
                     SPKAKARRAVSLSLLAGDDTVLTWAGELLAPTSSEKSLFTAAKTARERFAGAPRSLLF
                     DARSGYLSELVSDVVTHRGRLGRRWSQRIGQWCLRPWPGYWIGAGILYVVYLFVGVFG
                     AGTAVNFLEKTVFGEYITPGATRALAALHTPAFVLDLLVGPYGVVSMAFSYAFALILP
                     IVTTFFIAFGLLEDSGYLPRLSVLLDRVFRVMGLNGRAVLPVILGLGCDTMATMTTRI
                     LDTKKERVIVTMLLALSIPCSAQLAVILGMTAGLSPRVLVLWLAVLIGVTLASGWAAS
                     RVLPGARSTFLMEIPPIRRPLFRNILMKMKTRLAWYLKEVVPLFVWGTLGLFFLDRWG
                     WLKTAERLFAPVVQGWLGLPARATEAFLVGFLRRDYGAAGLYRLQQEGLLNLRQVTVS
                     LVLITLFMPCIAQWLMMFKERGWRAALGLTAAVMAVALAVSGALNALLLAFPALVG"
     gene            1838..2536
                     /locus_tag="PK362_05370"
     CDS             1838..2536
                     /locus_tag="PK362_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC74299.1"
                     /translation="MTDTQKYPIPTAAREDLEEALGLVWQQREDGVTDAATLRRLMNE
                     GVEPGAYARVIAEGYARENGPRLELTPPGEALGADVSRRHRLAERLLTDVLSLDHGAV
                     DPNACRLEHIISPEVSESICTLLGHPKECPHGHAIPPGPCCVRVEKHLEPIVVPLSRL
                     RPGDRGTVAYLQLREHPELHRLLSLGLIPGAALHLHQTFPAFVVDLGESQLALEESVA
                     DRIYVRRSADLQIS"
     gene            2570..>2695
                     /locus_tag="PK362_05375"
     CDS             2570..>2695
                     /locus_tag="PK362_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNC74300.1"
                     /translation="MPLAPLTRPIVFFDLETTGLWADVDRIIEIALLKRYPDDRVE"
BASE COUNT          405 a          907 c          842 g          541 t
ORIGIN      
        1 agcacggtgg ccaccaccgg cgacgggctg gccgagctca agcgcgccct ggaacacccc
       61 acgtgctcca acgtggcccc gccggacaac cccgacatcg cccgggcgct ggcggccctc
      121 gcgccgcatt tttcgccgaa ggccaaggcc cgtcgggccg tgagcctttc gctcctggcc
      181 ggggacgaca cggtcctgac ctgggcgggg gagctgttgg ccccgacttc ctcggaaaaa
      241 agtcttttca cggccgccaa gaccgcccgg gagcgcttcg ccggggcccc gcggtccctg
      301 ttgttcgacg cccggtccgg ctacctctcc gaactggtgt ccgacgtcgt cacccaccgg
      361 ggccgcctgg ggcgccgctg gtcccagcgc atcggccaat ggtgtctgcg gccctggccg
      421 ggctattgga tcggggccgg gatcttgtat gtcgtctatt tgtttgtcgg ggtcttcggg
      481 gcgggaacgg cggttaattt cctggaaaaa accgttttcg gggaatacat cacccccggc
      541 gcgacccggg ccctggccgc cctccacacg ccggccttcg tcctggacct cctggtcggt
      601 ccctacgggg tggtgagcat ggccttttcc tacgccttcg ccctcatcct gcccattgtc
      661 accaccttct ttatcgcctt tggattgttg gaggactccg gctatcttcc gcgcctctcg
      721 gtgctgttgg accgggtgtt ccgcgtgatg gggttgaacg gccgggcggt cctgcccgtg
      781 atacttggat tggggtgcga caccatggcc accatgacga cccgcatcct cgataccaaa
      841 aaggagcggg tcatcgtgac gatgctcctg gccctctcga tcccctgctc ggctcaattg
      901 gcggtcatcc tgggcatgac cgcgggcctc tcgccccggg tgctggtcct gtggctggcg
      961 gtgttgatcg gcgtgaccct ggcctccggc tgggcggcct cccgggtgct ccccggggcc
     1021 cgatcgactt ttttaatgga aataccgccc atccgccgcc cgctcttccg caacattttg
     1081 atgaagatga aaacccgctt ggcctggtat ttgaaagagg tcgtgcccct cttcgtgtgg
     1141 ggaacgttgg gtctcttctt cctggaccgg tggggctggt tgaaaacggc ggagcggctt
     1201 ttcgctcccg tggttcaagg atggctgggc cttccggccc gggccaccga ggctttcctg
     1261 gtcggattcc tccgtcggga ttacggcgcc gccgggctgt accggcttca acaggaaggc
     1321 ctgttgaacc tccgtcaggt cacggtgagt ctcgtcctca tcacgctttt tatgccctgc
     1381 atcgcccaat ggctcatgat gtttaaggaa cgcgggtggc gggcggccct gggactcacg
     1441 gccgcggtga tggccgtggc cctcgccgtg tcgggggcct tgaacgccct tttgctggcc
     1501 tttcccgcgc tggtgggcta gtgtagtgtc ttgcaaatcg cttgacaatg ggaggcccga
     1561 gtccgcactg gattcgaggc gcgaggaggg agcgatgcgg gccatcgtga ccaacgagca
     1621 acgaagaagc cggtcggagg cgggcctccc caccatcgaa tacgcctcgc caaggcgagg
     1681 agtttattgg aggaagggcc gggggctttt gcgcggcgag cggcgtctct cctcggtcac
     1741 atactcccgg tatgctccct cgtcgttcct agtctccacc caaaatcccc cggcccattg
     1801 ccaagggatt tgtaagacgc tacactaaaa tccctttatg accgacaccc agaaataccc
     1861 gattcccacc gccgcccggg aggatttgga ggaagccctc gggctcgtct ggcaacaacg
     1921 ggaggacggg gtgaccgacg ccgccaccct ccgccggttg atgaacgagg gcgtggaacc
     1981 cggcgcctac gcccgggtga tcgcggaagg ctacgcccgg gaaaacggcc cccggctgga
     2041 attgacgccc ccgggcgaag ccctgggcgc cgacgtgtcc cggcgccatc gattggccga
     2101 acgccttttg accgacgtgt tgagcctgga ccacggggcc gtcgatccga acgcctgccg
     2161 tttggaacac atcatctcgc ccgaggtgag cgaatccatt tgcacgttgc tgggccatcc
     2221 caaggaatgt ccccacgggc acgccatccc gcccgggccc tgctgcgtcc gggtggaaaa
     2281 gcatttggaa cccatcgtcg tccccctgag ccggctccgg cccggagacc ggggcacggt
     2341 ggcgtacctg cagcttcggg agcacccgga gctccaccgc ctgttgtccc tgggcctgat
     2401 ccccggggcc gccctccatt tgcaccagac cttccccgcc ttcgtcgtcg atttggggga
     2461 aagccagctg gccctggagg aatccgtggc cgatcgaatc tacgtgcggc gctccgccga
     2521 cctccaaatt tcctaaactc tttctttcag tcctccgtgg aaaggtttta tgcccctcgc
     2581 tccgttaacg cgtcccatcg tttttttcga tctggaaacc accggccttt gggccgacgt
     2641 ggaccggatc atcgaaatcg cccttctgaa acgctacccc gacgaccggg tggag
//
LOCUS       DAOQLS010000239         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22473_length_2693_cov_3.958681, whole genome shotgun sequence.
ACCESSION   DAOQLS010000239 DAOQLS010000000
VERSION     DAOQLS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22473_length_2693_cov_3.958681"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /gene="polA"
                     /locus_tag="PK362_05380"
     CDS             <1..1724
                     /gene="polA"
                     /locus_tag="PK362_05380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNC74301.1"
                     /translation="LDPFACALVGIALATRPGEAWYVPVGHRGLGEPPSWPLEKIRPV
                     LGPPLSDPKLPKVGQNLKFDGHVLERHGLPLVGVSFDTLVASYCLNPSRPNHGLKTLA
                     LDLLGESMTPIENLIGSGAKALTMDQVPVERAALYAGADAEVTLRLKALMEKDLRDHR
                     MDRLFHDVEMPLVGILSDMETAGLCLNVPYLQKLSREFGESLAGLEKEIHGLAGETFN
                     VNSPKQLAVVLFEKLKLPVLRKTKPGYSTDEEVLQTLSDRHPLPARVVAYRELAKLKS
                     TYVDGLLELVDPQTHRVHTRLNQAVAATGRLSSSDPNLQNIPIRTDAGRKIRRAFVPS
                     EGNVFLSADYSQIDLRVLAHMSGDPALVRAFRRGEDVHAATARDIFDLGPDAPVTDDQ
                     RRVAKSVNFGIVYGQTPFGLSQQLKIPLPQAQEYIRRYLEKYAGVQAWVEATLERARR
                     DGYVTTLLHRRRYLPEINASNGNVRAFAERTAMNTPIQGTSADIIKVAMREVTAWMAK
                     HGGRARLLLQVHDDLLFDVPRGELAALAPAVRRLMESAVPLDVPVVVDLKTGPNWADM
                     KKGEPAA"
     gene            1749..2582
                     /gene="mutM"
                     /locus_tag="PK362_05385"
     CDS             1749..2582
                     /gene="mutM"
                     /locus_tag="PK362_05385"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNC74302.1"
                     /translation="MPELPEVETIRRDLAGLLVGHTIAAVRILDPRAVQGFGPNGSPR
                     RSVDPRAFAERLAGRRVTEVRRRGKYLAIALSDGFLLVHLRMTGQLIWGPPSPRARAI
                     VEFRGVPAVLNYCDTRRFGELWTVDRLEDDPALAALGPEPLADFDVNAWGRALRRSDA
                     RVQAALLDQRRIAGLGNIYVTEALFLTGVRPTRRCGRLAPAEIPPLVRNIRRVLSTGI
                     RRRGVSFRDYRDARGERGQARATLNVYGKAGRPCPRCRSPLRGVKVNGRGTVYCGRCQ
                     R"
     gene            2579..>2693
                     /locus_tag="PK362_05390"
     CDS             2579..>2693
                     /locus_tag="PK362_05390"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNC74303.1"
                     /translation="MIKKRIRVIGLTGGVGSGKSTAAAAFRARRIPVVDADQ"
BASE COUNT          444 a          924 c          864 g          461 t
ORIGIN      
        1 gcctggatcc cttcgcctgc gctctggtcg ggatcgccct cgctacccgc cccggggagg
       61 cctggtatgt gcccgtgggt catcggggtt tgggggagcc gccttcctgg cccctggaga
      121 aaattcggcc cgtcctgggc ccgcccttgt ccgacccgaa gcttcccaag gtgggtcaaa
      181 atctgaaatt cgacggccac gtgctcgagc gccacgggct ccccctggtc ggcgtgtcct
      241 tcgacacgct ggtggcctcc tattgcctga acccctcccg gcccaatcac ggattgaaaa
      301 ccctggccct cgatctcctg ggggaatcca tgacgcccat cgagaacctg atcggttccg
      361 gcgccaaggc cctgaccatg gaccaggtcc ccgtcgagcg ggccgccctc tacgcggggg
      421 ccgatgccga ggtgaccttg cgcctgaaag cgttgatgga aaaagacctt cgcgaccacc
      481 gcatggaccg gctctttcac gacgtggaaa tgcccctggt cggcatcctc tccgacatgg
      541 agaccgccgg actgtgcctc aacgtgccct acctgcaaaa attgtcccgg gaattcggcg
      601 aatctctggc gggcctggaa aaggaaattc acgggctggc cggggaaacc ttcaacgtca
      661 attcgcccaa acagttggcg gtggtgttgt ttgaaaaatt gaaactgccc gtgctgagaa
      721 aaaccaagcc cggctattcc accgacgaag aagtgcttca aacgctctcc gaccgccacc
      781 cgttgccggc ccgggtcgtg gcctaccggg agctggccaa actcaaatcc acctacgtcg
      841 acggcctcct ggagctcgtg gatccccaaa cgcaccgggt ccacacccgg ttgaaccagg
      901 ccgtggcggc caccggacgc ctgtcctcga gcgaccccaa tcttcaaaac attccgatcc
      961 gcaccgacgc gggccgcaaa attcggcggg ccttcgtgcc ctccgaggga aacgtcttcc
     1021 tgtcggccga ttactcccag atcgacctcc gcgtgctggc ccacatgtcg ggggacccgg
     1081 ccctggtccg ggccttccgc cggggggagg acgtccacgc cgccaccgcc cgggacatct
     1141 tcgatctcgg gcccgacgcc ccggtgaccg acgatcaacg ccgcgtggcc aagtccgtta
     1201 atttcggcat cgtctacggt caaaccccct tcggcctgtc gcagcaattg aaaattcccc
     1261 tgccccaggc ccaggaatac atccgccgct atttggaaaa atacgccggc gttcaagcct
     1321 gggtggaggc cacgctggag cgggcccgcc gggacggtta cgtgaccacc ctcctccacc
     1381 gtcggcgcta ccttcccgaa atcaacgcct ccaacggcaa cgtgcgggct ttcgccgagc
     1441 ggacggccat gaacaccccg atccagggaa ccagcgccga catcatcaag gtggccatgc
     1501 gcgaggtgac ggcttggatg gcaaaacacg gcggacgggc ccggctgttg cttcaagtgc
     1561 acgacgattt gctcttcgac gttccccggg gggaattggc ggccctggcg ccggcggtcc
     1621 ggcgtctcat ggagtccgcg gtgccgttgg acgtccccgt ggtggtcgac ctgaagacgg
     1681 gccccaattg ggccgatatg aaaaaggggg agcccgccgc gtgatccgcc ccccgaaggc
     1741 cggggcccgt gcctgaactg ccggaggtgg aaaccatccg ccgggatctg gccgggctgt
     1801 tggtcggcca caccatcgcc gccgttcgaa tcctcgatcc ccgggcggtc caggggttcg
     1861 gaccgaacgg gagcccccgc cggtccgtcg atccgcgggc cttcgccgaa cggttggcgg
     1921 gccgccgcgt cacggaggtg cggcgccggg ggaaatacct ggcgatcgcc ctgtccgacg
     1981 gtttcttgct ggtgcattta cggatgacgg gccaattgat ctgggggccc ccgtcgcccc
     2041 gggcccgggc catagtggaa tttcgcgggg tccccgccgt tctgaattac tgcgacacgc
     2101 gtcgctttgg ggaattgtgg accgtggacc gcttggaaga cgatccggcc ctcgcggcgt
     2161 tgggccccga gccgttggcg gatttcgacg tgaacgcctg ggggcgcgcc cttcgccggt
     2221 ccgacgcccg ggtccaggcg gccctgctcg atcaacggcg gatcgccggc ctcggaaaca
     2281 tttacgtgac cgaagccctg tttttgaccg gcgtccggcc cacgcgccgt tgcggccgcc
     2341 tcgctcccgc ggaaattccg cccctcgtgc ggaacatccg ccgggtgttg tcgacgggca
     2401 tccgtcggcg gggcgtgtcg ttccgggatt accgcgacgc ccggggcgag cgcggccagg
     2461 cccgggccac cttaaacgtg tacggcaaag cgggtcggcc ctgcccccgc tgccgatcgc
     2521 ccctgcgggg ggtgaaggtc aatggacggg gaaccgttta ttgcggtcgg tgccaacgat
     2581 gataaaaaaa cggattcggg tcatcggttt gacgggcggc gtcggctccg gaaagtccac
     2641 cgccgccgcg gcgtttcggg cccggcgaat tccggtggtg gacgcggacc aaa
//
LOCUS       DAOQLS010000240         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22524_length_2690_cov_3.776471, whole genome shotgun sequence.
ACCESSION   DAOQLS010000240 DAOQLS010000000
VERSION     DAOQLS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22524_length_2690_cov_3.776471"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PK362_05395"
     CDS             <1..1527
                     /locus_tag="PK362_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNC74304.1"
                     /translation="LLKDTYGIIVYQEQVMQIAQVLAGYTPGQADLLRKAMGKKIQEI
                     LDQQRDTFLKGCKDNNVDKKIAEKVFDLVVQFGGYGFNKSHASAYGLVSYQTAFLKAN
                     YPTEFMAAVLTSEIGHSNLGSKEVESKLVTYMGEAQEMGIEILQPDVQKSVDVFSIET
                     PPTEKESPAIRFGLLAVKNVGDGAVASLLEARRTKGPFASLDDLCARVDTRQVNRKVL
                     ESLVKAGALDGFRPVLGTESDEVRLLELCRWRSQLHGDVGDTLGRATSAREETAVGQG
                     ALFDLGGVSKGPPRAQNGNAPAEWSEHELLANEKEVLGFYLSGHPLARYRRELASYTT
                     HTLGKLPESGIVRVAGMIVNTKKTVTKSGHAMSRFKLEDLEGEVECVVFPKTYTADLA
                     RALVAHEMIVVKGRVETRMDEKNILVEEAVPLRDARARFVKRVVVRLSTAGLAEDRVA
                     KIQRAAAAHPGACRLCFELSTPTHGTYRLATEHKVAPTDGFVHEMENLLGRGSVDLQA
                     "
     gene            1605..2651
                     /locus_tag="PK362_05400"
     CDS             1605..2651
                     /locus_tag="PK362_05400"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNC74305.1"
                     /translation="MKKYRVAVVGAGAVGVQMVRILNQRKFPVEELRVLARSARTLDI
                     DGRSYAVQETTAEAFDGVDIALFAGTEGEKGAAVTFGAEAVKRGAVVIDNGSDFRMKD
                     DVPLVIPELNADALKNHKGIIANPNCTTAIILMGLGPLHKAFGVTRAVISTYQAVSGA
                     GAKGVEGLRKQTEAALKGQTVSDLNGFPNPIAFNVLANNWAIEEEGYSNEEWKVVKET
                     HKILGDDSIRVAVTTVRVPTYVGHGESVWIETKKPITPEAARAVFAAAPGVRVVDEAS
                     PLEGVNCPMPLLAEGKDDSLVGRIRRDLFSDHALAFWVVGDNLRKGAALNAVQIAETL
                     AARGWLKPSVPAGR"
BASE COUNT          521 a          896 c          855 g          418 t
ORIGIN      
        1 ctcctcaagg acacctacgg catcatcgtg taccaggagc aggtcatgca aatcgcccag
       61 gtcctggccg ggtacacccc gggccaagcg gacctgctcc ggaaggccat gggcaaaaaa
      121 atccaggaaa tcctggacca gcaacgggac accttcctca agggatgcaa ggacaacaac
      181 gtcgacaaga aaatcgccga gaaagtcttc gacctggtgg tccagttcgg cggctacggc
      241 ttcaacaaga gccacgcctc ggcctacggg ctcgtcagct atcaaacggc gttcctgaag
      301 gccaattacc cgacggaatt catggccgcg gtgttgacgt cggaaatcgg ccattccaat
      361 ttggggtcca aggaggtgga gtccaagctc gtcacctaca tgggcgaggc ccaggaaatg
      421 ggcatcgaaa tcctccagcc cgacgtgcaa aaatcggtcg acgtgttttc catcgaaacc
      481 ccgccgacgg aaaaggaaag ccccgccatc cgcttcggcc ttttggcggt gaaaaacgtc
      541 ggcgacggcg ccgtcgcttc gctgctggag gcccggcgca ccaagggccc cttcgcctcc
      601 ctggacgatc tctgcgcccg ggtggacacc cgccaggtca accggaaggt cctcgaatcc
      661 ctcgtcaaag cgggggccct ggacggtttc cgccccgtgc tggggacgga atccgacgag
      721 gtccgcctgt tggaattgtg ccgctggcgg tcccaattgc acggcgacgt gggcgacacc
      781 ctggggcggg ccacaagcgc ccgggaggaa accgcggtgg gccagggcgc cctgttcgac
      841 ctgggcgggg tctccaaggg gccgccccgg gcgcaaaacg gcaacgcccc cgccgagtgg
      901 tcggagcacg aactgctcgc caacgaaaag gaagtcctcg ggttttatct ttccggccat
      961 cccctggcgc gctaccgccg ggagctcgcc agttacacca cccacaccct cgggaaactg
     1021 cccgaaagcg gcatcgtgcg ggtggccggc atgatcgtca acacgaagaa aaccgtcacc
     1081 aaatccgggc acgccatgag ccgttttaaa ctggaggacc tggaggggga agtggaatgc
     1141 gtggttttcc ccaaaaccta caccgcggat ctggcccggg ccctggtggc ccacgagatg
     1201 atcgtggtca aaggccgggt ggaaacccgc atggacgaga agaatatctt ggtggaggag
     1261 gcggtgccgc ttcgggacgc ccgggcccgg ttcgtcaaac gggtggtcgt gcgcctctcc
     1321 accgccgggt tggccgagga ccgcgtggcc aagatccagc gggccgccgc ggcccacccc
     1381 ggcgcctgcc gcctttgctt cgaactgtcg acgccgaccc acggcactta ccggctggcg
     1441 accgagcaca aggtggcgcc caccgacggc ttcgtccacg agatggagaa cctgctgggg
     1501 cgcggctccg tcgacctcca ggcgtgacgc cggggagaaa tttgttataa ttgccgcggg
     1561 cggcctttcg gggctcgcct tttttgttgc ccgaggagag gatcgtgaaa aaatatcgtg
     1621 tggcggtggt gggcgccggc gccgtcggcg ttcaaatggt tcggatttta aaccagcgga
     1681 aatttcccgt ggaggaactc cgcgtcctgg cgcgcagcgc ccggaccctg gacatcgacg
     1741 gccgttccta cgccgtccag gaaacgaccg ccgaggcctt tgacggcgtc gacatcgccc
     1801 tcttcgccgg caccgaaggc gaaaaaggcg ccgccgtcac cttcggcgcc gaggccgtga
     1861 agcggggcgc cgtggtgatc gacaacggtt cggatttccg catgaaagac gacgtgccgc
     1921 tcgtgatccc cgagctcaac gccgacgccc tcaaaaacca caagggcatc atcgccaacc
     1981 ccaattgcac gacggccatc atcctgatgg gcctgggccc cctgcacaag gccttcggcg
     2041 tgacccgcgc cgtgatttcc acctaccagg ccgtttccgg cgccggcgcc aaaggcgtcg
     2101 aggggcttcg aaaacagacc gaggccgcct tgaagggcca aaccgtctcg gacctgaacg
     2161 gatttcccaa ccccatcgcc ttcaacgtgt tggcgaacaa ctgggccatc gaggaagagg
     2221 ggtattccaa cgaggaatgg aaagtcgtca aggaaaccca caaaatcctg ggcgacgatt
     2281 ccatccgcgt cgcggtcacg acggtccggg tgcccaccta cgtgggccac ggcgaatccg
     2341 tctggatcga aaccaagaag cccatcacgc ccgaggcggc ccgcgcggtg ttcgcggcgg
     2401 cccccggcgt ccgcgttgtg gacgaggcgt cgcccctgga gggcgtcaac tgccccatgc
     2461 cgctcctggc cgaaggcaag gacgattccc tggtcggccg catccggcgc gacctcttca
     2521 gcgaccacgc cttggcgttc tgggtcgtcg gcgacaatct gcgcaagggc gcggccctga
     2581 acgccgtgca aatcgccgaa accctggcgg cccgggggtg gctcaaaccc tccgtacccg
     2641 ccggccgctg acccgcgcgt gaaggtcctc ctcttcggcg gcgcgggcca
//
LOCUS       DAOQLS010000241         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22528_length_2690_cov_2.541935, whole genome shotgun sequence.
ACCESSION   DAOQLS010000241 DAOQLS010000000
VERSION     DAOQLS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22528_length_2690_cov_2.541935"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK362_05405"
     CDS             <1..296
                     /locus_tag="PK362_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74306.1"
                     /translation="APVPRFPKNEAEYESARRRYLNTYGKFASYAVVRTERGPLEVLV
                     EKGTLLVMAVNGQPLKPEHIGLTVENDVRWSSPASSDGQGDAAPAYPEPGPPS"
     gene            293..649
                     /locus_tag="PK362_05410"
     CDS             293..649
                     /locus_tag="PK362_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74307.1"
                     /translation="MIKSPAKKCLVVRKGEKNALLLEEGNAAKSRFRLPRLEEIAPGP
                     LAAGAAVPVGANLVLLFTVKELSPWELMKDFPPPKYFDYAYLDLLDPEGPDWDRLTGE
                     DRDLISRARTTISQSL"
     gene            671..1966
                     /locus_tag="PK362_05415"
     CDS             671..1966
                     /locus_tag="PK362_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74308.1"
                     /translation="MIRILKKTLRGMTAGFLLGGAPSSPAAPWTSWTKHPTNPVYTHG
                     TVASDPAVLKDGSTYRMIVSGEGGGSPDGNSLIEATSPDGTRWTTLPNGQNGRVVPSA
                     PGVWDESLETPELIKAGGEYLIFYAGYDPAVRDASGGLAWGDLGLAVSADGTHFARSG
                     PAPVLTRTPNSFDQDGLTDPTIVEVGGTLEMIYVGWCTQGCAINGGAPAFYALKAVST
                     DNGRTWTKQGRLDPGSNIGLQHPDLVLDADGLYSLFYGVDGACGGGRVGLSLARGPTA
                     FGPFTEASAAPVLCLGTRGFENAGTDGGFPSVLNDGGVGRMYYTGVNEALFEFQIGLA
                     ETRLTVPGGGADPVGFPNPFHPSADQSMTFARLAPGGRLGIYDLTGRPVIEVSADGGG
                     AAQWDGRDGSGRRVASGIYWGLADGVDGTRRSFKIVVRE"
     gene            complement(2008..>2690)
                     /locus_tag="PK362_05420"
     CDS             complement(2008..>2690)
                     /locus_tag="PK362_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74309.1"
                     /translation="MESFHPVNGDPVLAEASAAVNLAEDLIVFNRFPTGLPRELFYSS
                     RTAVGWSTPMAVPGVASACDDDNGFVVGDLLHGATFYFESRRVDPAAGSCGSLHRICR
                     TYFPPAGPNTPVEFLPGLGTADDDTQISVSPDQKEIYWTSVRGGKYGIFTADLQANGS
                     YGNIRPVVTGVTASPYRGKLTFMGEANIARVPQGELMYLMCGIAGGDSNGYPINLRLK
                     ICQARRTR"
BASE COUNT          500 a          807 c          897 g          486 t
ORIGIN      
        1 aggcgccggt tccccggttt cccaaaaacg aagcggaata tgaaagcgcc cgacggcgct
       61 atttgaacac gtacggaaaa ttcgcctcgt acgccgtggt gcggaccgaa cggggacctt
      121 tggaggttct ggtggaaaag gggacccttc tggtgatggc cgtcaacggc cagccgttaa
      181 aaccggaaca tattggattg accgtcgaaa acgacgtgcg gtggtcttcc ccggcgtcct
      241 ccgacggcca gggcgacgcg gcgcccgcct atcctgaacc gggcccgccg tcatgatcaa
      301 gtcgcccgcc aaaaaatgcc ttgtggtcag gaagggcgag aaaaacgctc ttctgttgga
      361 ggaggggaac gcggccaaaa gccgattccg ccttccccgg ctggaggaaa ttgcgcccgg
      421 ccctttggcc gcgggcgccg ccgtcccggt gggggccaac cttgtcctcc tttttaccgt
      481 taaggaattg tcgccctggg aactaatgaa ggatttcccg cctcccaagt atttcgacta
      541 cgcctatttg gatctcctcg accccgaagg gcccgattgg gaccggttga ccggagagga
      601 ccgggatttg atttcccggg cgcggacgac gatctcccag tccctttaat cgccgaagga
      661 aaccgccccc ttgatccgaa ttttgaagaa gacgcttcgg ggaatgaccg cgggcttttt
      721 gcttggtggg gcgccaagtt ccccggccgc gccctggacc tcctggacca aacacccgac
      781 caatcccgtt tacacccacg gcaccgtcgc ctccgacccc gccgtcctaa aagacgggtc
      841 cacctatcga atgatcgttt cgggcgaagg cggcggtagc cccgacggca acagtttgat
      901 cgaggcgact tccccggacg gcacccggtg gacgacgctt cccaacggac aaaacggccg
      961 ggtggtgccc agcgccccag gggtgtggga cgaaagcctc gaaacgcccg agttgatcaa
     1021 ggcggggggg gaatatttga ttttttacgc cggctacgac ccggcggtcc gggacgcctc
     1081 cggcggcctg gcctggggcg acctgggact ggcggtttcc gccgacggaa cacatttcgc
     1141 ccggtccggc cccgcgccgg ttttgacccg cacgccgaac tcttttgacc aggacggcct
     1201 caccgacccg accatcgtcg aggtcggcgg aacgttggaa atgatttacg tggggtggtg
     1261 cacgcaaggt tgcgccatca acggcggggc cccggcgttt tacgctttga aggccgtgtc
     1321 gacggacaac ggacgcactt ggaccaaaca ggggcgattg gaccccggga gcaacatcgg
     1381 attgcaacac ccggacctcg tgttggacgc cgacggattg tattccctct tctacggggt
     1441 ggacggcgcc tgcggcggcg gacgggtggg cctttccctg gcccggggac cgaccgcctt
     1501 cggcccgttc acggaggcgt cggccgcccc cgtcctttgc ctcggcaccc ggggattcga
     1561 aaacgccggg acggacggag gtttccccag tgttctcaac gacggcgggg tgggccggat
     1621 gtattacacc ggggtcaacg aggcgttgtt cgaatttcaa atcggactgg cggaaacccg
     1681 attgacggtc ccgggcggcg gggcggaccc cgtgggattt ccgaatcctt tccatccatc
     1741 ggcggatcaa tcgatgacct tcgcccggct ggccccgggt ggacgcctgg gaatttatga
     1801 cttaacgggc cgaccggtga tcgaggtgtc ggcggacggc ggcggcgccg cccaatggga
     1861 cggacgggac ggttcggggc ggcgggtggc gagcgggatc tattggggct tggccgacgg
     1921 ggtcgacggg acgaggagat cttttaaaat cgttgtccga gagtgacccg cggatggacg
     1981 ggcggagtcc gaagccgaat tctttttcta acgcgtccgt cgcgcctggc atattttcag
     2041 gcggaggttg atggggtaac cgttgctgtc gccgcccgcg atgccgcaca tcaaatacat
     2101 caattcgccc tggggcaccc gggcaatgtt ggcctctccc ataaacgtca gttttccccg
     2161 atagggggaa gcggtgacgc cggtcaccac cggacggatg tttccgtagg agccgttggc
     2221 ctgcaaatcg gccgtgaaga tgccgtattt tccgccgcga acgctggtcc aataaatttc
     2281 cttttggtcc ggcgaaacgg aaatctgcgt gtcgtcgtcc gcggttccca gccccggaag
     2341 aaattcaacg ggggtgttgg ggccggcggg ggggaaatac gtgcggcaga ttcgatggag
     2401 ggaaccgcag gagcccgcgg cggggtcgac gcgtcgggat tcgaagtaaa acgtggcgcc
     2461 gtgaagaagg tcgcccacca caaagccgtt gtcgtcgtcg caggccgagg caacgcccgg
     2521 gacggccatc ggcgtcgacc agccgacggc cgttcggctg gaataaaaga gttcccgggg
     2581 tagaccggtc ggaaaccggt taaagacaat cagatcttcg gcgagattga cggcggccga
     2641 ggcttccgcg aggacgggat cgccgttaac ggggtggaag gattccatcc
//
LOCUS       DAOQLS010000242         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22751_length_2676_cov_3.159863, whole genome shotgun sequence.
ACCESSION   DAOQLS010000242 DAOQLS010000000
VERSION     DAOQLS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22751_length_2676_cov_3.159863"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..532
                     /gene="tpx"
                     /locus_tag="PK362_05425"
     CDS             11..532
                     /gene="tpx"
                     /locus_tag="PK362_05425"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNC74310.1"
                     /translation="MSNERKGGTTFKGNPLTLVGNEIKVGQKAPDFKAVAGDLSEVNL
                     AKSAGKTRLLVAVPSLDTPVCDAETRKFNEAVSKVSGVQAYVISMDLPFAQGRFCQTA
                     GIKNLQPLSDHRDASFGVAYGTLIKELRLLSRAVFVVNAQDVVTYVEYVPEMTNHPNY
                     DAALAALRQPAGV"
     gene            632..705
                     /locus_tag="PK362_05430"
     tRNA            632..705
                     /locus_tag="PK362_05430"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:664..666,aa:Gln,seq:ctg)
     gene            complement(754..1062)
                     /locus_tag="PK362_05435"
     CDS             complement(754..1062)
                     /locus_tag="PK362_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNC74311.1"
                     /translation="MKADPPPFPILVYVCTNARASGERVSCAAAGRDGLRLKDELKRL
                     VKEGGWSDRIRICSSGCMDRCEEGPNAVRLGASGNEWISHLASGDLPALVERLAQNLP
                     "
     gene            complement(1064..2155)
                     /gene="mqnC"
                     /locus_tag="PK362_05440"
     CDS             complement(1064..2155)
                     /gene="mqnC"
                     /locus_tag="PK362_05440"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNC74312.1"
                     /translation="MSELGTLEQKILRGDRLTPEEGRRLLTEEVPLLELGAWAHRVRQ
                     TKNPGREVSFVIDSNPNYTNVCDTDCLFCAFYRRPKDPDAYALTVEQVMEKVRSAVDR
                     GATTILLQGGHNRTLPLSYYTEIVRTMKQRFPSVTPHCFTASEIQTMAQVSGLTIDGV
                     LNELWEAGQRTLPGGGAEILSESVRQKLAHKKGGPEKWLEVHRAAHRRGFRSTATMMY
                     GHVETLDDIVEHWERIRALQDETHGFTAFIPWSFKPQNTVLERRHPERTGPVPYLRVL
                     AASRVYLDNFDHIQASWFSEGKKAGQASLHFGADDFGGTLIDENVHAAAGFVNKASTD
                     DVVAFIREAGFVPVQRSTLYDVLKTFGPN"
     gene            complement(2148..2300)
                     /locus_tag="PK362_05445"
     CDS             complement(2148..2300)
                     /locus_tag="PK362_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74313.1"
                     /translation="MCRQAARALRLPASRMEDYYDGLVYRLTESERRGEALFRDVIAG
                     DEKVRV"
     gene            2340..2507
                     /locus_tag="PK362_05450"
                     /pseudo
     CDS             2340..2507
                     /locus_tag="PK362_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT          511 a          810 c          827 g          528 t
ORIGIN      
        1 gaggtaatcc gtgtcgaacg aacgaaaagg cggtacgact tttaaaggca atcctttaac
       61 gctggtcggc aacgaaatca aggtgggtca aaaagccccg gatttcaagg ccgtggcggg
      121 agatttgagc gaagtgaatc tggccaaatc cgccgggaaa acgcgtttgc tcgtggcggt
      181 gccgtccctg gacacgcccg tctgcgacgc cgaaacccgg aaattcaacg aagcggtgtc
      241 caaagtctcc ggcgttcagg cctacgtcat cagcatggat ttgccctttg cccagggccg
      301 gttctgccaa accgccggca tcaaaaatct ccaacccctg tcggaccacc gggacgcttc
      361 cttcggcgtg gcctacggaa cactgatcaa agagcttcga cttctctccc gggccgtctt
      421 tgtcgtcaac gcccaagacg tggtcaccta cgtggaatac gtgccggaaa tgaccaacca
      481 ccccaactac gacgcggcgc tggcggccct gcgccaaccc gccggggtgt aagcgtttcg
      541 tcgcggattc tcatcggtag cgggatttga taaaatcccc ttcggttttc cgaaggggat
      601 tttatttttc tccgccggat cgcgcgattt ttgccggatg aggtaacggt agcctgccag
      661 gctctgaacc tggtcgtcta ggttcgaatc ctagtccggc agccatctaa aaaaaggacg
      721 gttcctccgg gaaccgtcct tttttattgg gaatcaggga aggttttggg ccagccgttc
      781 aaccaaagcg ggcaagtccc cggacgccag gtgggaaatc cattcgttgc cggaggcgcc
      841 caagcgaacg gcgttggggc cctcttcgca gcggtccatg caacccgagg aacaaatacg
      901 aatccgatcg gaccatccgc cttctttgac gagacgcttt aattcgtctt tcagtcggag
      961 accgtcccgg ccggcggccg cgcaggacac ccgttcgccg gaagcgcgcg cgttcgtgca
     1021 cacgtaaacc agaatgggaa acgggggcgg gtcggccttc acgtcagttc gggccgaagg
     1081 ttttcaggac gtcgtaaagg gtggaacgtt gaacggggac gaagcccgcc tcccggatga
     1141 atgccaccac atcgtccgtc gaggctttgt tcacgaatcc cgccgccgcg tgaacgttct
     1201 catcgatcaa ggtgcccccg aaatcatcgg cgccgaaatg gagggaagcc tggccggcct
     1261 ttttgccttc cgaaaaccag gaggcttgga tgtggtcgaa attgtccaaa tagacgcgcg
     1321 aagcggccag cacgcgaagg tacggcacgg ggccggtccg ttcgggatgc cggcgctcca
     1381 aaacggtgtt ttggggtttg aaactccagg gaatgaaggc cgtgaatccg tgggtttcgt
     1441 cctgcaacgc gcgaatccgc tcccaatgtt caacgatatc gtccaaggtt tcgacgtggc
     1501 cgtacatcat ggtggcggtg cttcgaaacc cccgccggtg ggccgcccga tggacttcca
     1561 accatttttc cgggccgcct tttttgtggg cgagtttttg gcgaacggat tccgatagaa
     1621 tctccgcccc gccgccgggg agcgtgcgtt gcccggcctc ccataattcg ttcaggacgc
     1681 cgtcgatggt caaccccgac acctgggcca tggtttggat ttcggacgcc gtgaaacagt
     1741 ggggggtgac ggacgggaac cgctgtttca tcgttcggac gatctcggtg taatacgaaa
     1801 gggggagggt ccggttgtgg ccgccctgca ggaggatcgt ggtggcgccc cggtccaccg
     1861 cggaccggac tttttccatc acctgctcaa cggtcagcgc gtaggcgtcg gggtccttgg
     1921 gacggcgata aaaagcgcaa aaaagacagt ccgtgtcgca gacgttcgtg tagttgggat
     1981 ttgaatcgat gacaaaggaa acttcccggc ccggattctt ggtctgacgg acgcgatggg
     2041 cccaggcccc cagttcgaga agagggacct cctccgtcag caaacggcgg ccttcctcgg
     2101 gggtcaggcg gtcgccccgg agaatttttt gttccagagt cccgagctca gacacggact
     2161 ttttcgtccc cggcgatgac gtcccgaaag agcgcttcgc cccggcgttc gctttccgtc
     2221 aggcggtaga cgaggccgtc gtaatagtct tccattcggg aggcgggcaa ccgcagggcg
     2281 cgggccgcct ggcggcacat gttctcccga ttccgatcaa aggcctccag ggaggcttcc
     2341 agggcttctt cgagaagtcc ttccggctcg gtgagtcgga cctgcccaca cgactgcgtg
     2401 tcgagctcga ggccgtggag gccgaacgtc aggccgcgcg agcgcgcatc gaagccgccg
     2461 ccgccatctc cgccctgcgc caggcgctgg gactgcttcc tcaatgaatt cttcgaccat
     2521 gaacatcctt cgacacttca cgtcgctggg catcgtgctg atgctcggcg ccaccacgct
     2581 cgttgcgcga gcgggtgatg gccacgacca tggcgacgcg ccgccggcgg cgaccgggcc
     2641 ggccctgccg cgcttcgcgg cggtctccga acagtt
//
LOCUS       DAOQLS010000243         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22775_length_2674_cov_4.425353, whole genome shotgun sequence.
ACCESSION   DAOQLS010000243 DAOQLS010000000
VERSION     DAOQLS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22775_length_2674_cov_4.425353"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PK362_05455"
     CDS             complement(<1..229)
                     /locus_tag="PK362_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(hydroxyalkanoate) granule-associated
                     protein"
                     /protein_id="HNC74314.1"
                     /translation="MTDAKKKPAAAKSAAKAAPKKVDAKKKAKPAAEKPVKAKRVSKK
                     DPDAAKAAPTAAVVVKIKKEPKPEPAPVSVKP"
     gene            complement(234..1607)
                     /gene="nusA"
                     /locus_tag="PK362_05460"
     CDS             complement(234..1607)
                     /gene="nusA"
                     /locus_tag="PK362_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNC74315.1"
                     /translation="MNKSELIPVLEQIEKDKGIPKEEILKMIEQALVSAYRKHAGQQV
                     NVQASIDASSGLIRAFVVKAIVETVANPAVEITAAEAKKVRSGIIEGAELKIPVEAED
                     FARIAAQTAKQVLIQKIRETERESLYSEYKPKEGILINGTVHRFANRNLIVDMGRNEG
                     VIPMREQVRREHWMVGDRVRALILKVEKGPRGPEVILSRAHPDFVKRLFEQEVPEIYE
                     KTVEVLDVVREAGFRSKVVVRSNNPKVDPIGACVGVKGSRVRPIINELQGERIDLVAY
                     SPDVTTYIANALSPVKPLSVRIVNLEEKRAEALVANDQLSLAIGKAGQNVRLANKLTG
                     WNIDVRSELQKKEESQATAAARIDELSRLEGVGPKTAEVLQKGGWGDMERLANAKPDD
                     LTALSGVGEKTAEKIIESAKLYLQAKAAAPADAATDEAAADTAPAETPESAPAPESEP
                     TAGAKDE"
     gene            complement(1627..2088)
                     /gene="rimP"
                     /locus_tag="PK362_05465"
     CDS             complement(1627..2088)
                     /gene="rimP"
                     /locus_tag="PK362_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNC74316.1"
                     /translation="MGVAENIENNLAPVLKEAGVELVAVQYQREPVGWVLRFFLDKEG
                     GISLDDCALWSDRLGTLVEERGLATGEYSLEVSSPGLQRPLRKREDFLRFTGLEAIVK
                     TFAPINNQRNFHGVLRGVENDDLILTDRTNGLVKVPLKDIASAKLDPAVGA"
     gene            complement(2174..>2674)
                     /locus_tag="PK362_05470"
     CDS             complement(2174..>2674)
                     /locus_tag="PK362_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74317.1"
                     /translation="TLRHDLFLFSSAALRRQGPSREFRWATVDSVAGLDLSAAWLNSN
                     QNNYDTSRTVFNNDYYDYVEWTAGPRVSLRFNQKLSVGLGAAWSRRRYGRRPVQDADG
                     TYLSGNVTTTAWTYRVSLGYPLMEHLDAVLTGAYQDASSNMKYESVYRYNYSSASYFG
                     GLSLKI"
BASE COUNT          444 a          747 c          864 g          619 t
ORIGIN      
        1 ggggcttgac ggaaaccggc gccggttcgg gtttcggttc ttttttgatt ttgacgacga
       61 ccgcggcggt cggcgccgct ttcgccgcgt ccggatcttt tttcgacacg cgcttcgcct
      121 tgaccggttt ttcagcggcc ggtttggctt ttttcttggc gtcgactttc ttgggggcgg
      181 ctttggcggc cgacttggcg gccgcgggtt tctttttggc gtcggtcatg gcgttattcg
      241 tccttggctc cggcggtcgg ttcggactcc ggcgcgggcg cggattcggg tgtttcggcc
      301 ggggccgtgt cggcggcggc ctcgtcggtc gcggcgtcgg cgggggcggc cgctttggcc
      361 tgcaaataca atttggcgga ttcgatgatt ttctccgccg tcttttcacc cacgccggaa
      421 agggccgtga ggtcgtcggg cttggcgttg gccaggcgtt ccatgtcccc ccagccgcct
      481 ttttggagca cttcggcggt tttgggcccg acgccttcca accgggaaag ctcgtcgatg
      541 cgggcggcgg ccgtggcctg ggattcctct ttcttctgca attcggaccg gacatcgata
      601 ttccaaccgg tgagtttatt ggccaaacgg acgttttggc cggctttgcc gatggccagg
      661 gagagttggt cgttggccac cagggcttcg gcgcgctttt cctccaggtt gacgatgcgg
      721 accgagaggg gcttgaccgg cgacaaagcg ttggcgatgt aagtggtgac gtccggcgag
      781 taggcgacga ggtcgatgcg ttcgccctgg agttcgttga tgatgggccg gacccgggag
      841 cccttcacgc cgacgcaggc gccgatggga tccaccttgg ggttgttgct ccggacgacg
      901 actttggaac ggaaccccgc ctcccggacg acgtccagga cttcgacggt tttctcgtaa
      961 atttcaggga cttcctgctc gaacaggcgt ttcacgaaat ccgggtgcgc ccgggacaaa
     1021 atgacttcgg ggccccgggg ccccttctcg actttcaaga tgagggcgcg gacgcggtcg
     1081 ccgaccatcc aatgttcccg gcggacctgc tcgcgcatgg ggatgacgcc ttcgttgcgg
     1141 cccatgtcga cgatgaggtt tcggttcgca aaacggtgga cggtgccgtt gatgagaatg
     1201 ccttccttcg gcttgtattc ggaatagagg ctttcgcgct cggtttcgcg gattttttga
     1261 atgagcacct gcttcgccgt ttgggcggcg atgcgggcga aatcttcggc ctcgacgggg
     1321 attttgagct ccgcgccttc gatgatcccg gaccggacct ttttggcctc ggcggcggtg
     1381 atttccacgg cggggttggc gacggtttcc acgatggctt tgacgacgaa agcccggatc
     1441 aggcccgagg aggcgtcgat ggaggcttgg acgttgacct gctgccccgc gtgctttcgg
     1501 taagcggaga ccagggcctg ttcgatcatc tttaaaattt cttctttggg aatccccttg
     1561 tccttttcaa tttgctccag aacggggatc aattcgcttt tgttcatgtg atggtttacc
     1621 cttttcctag gcgccgaccg cggggtcgag tttcgcgctg gcgatgtctt tcagtggcac
     1681 tttgaccaaa ccgttcgttc ggtccgttaa aatcaaatcg tcgttttcga cgccccggag
     1741 gacgccgtgg aagttgcgct ggttgttgat cggggcgaag gttttgacga tggcctccag
     1801 gcccgtgaac cgaaggaaat cctcccgttt gcgcaggggc cgttgaaggc cggggctgga
     1861 gacttccagg gaatattccc cggtcgcgag accgcgttct tccaccaacg ttcccagacg
     1921 gtcgctccaa agggcgcagt cgtccaggga gatgccgccc tctttgtcca aaaagaaccg
     1981 gagcacccag ccgacgggtt cccgctggta ttgaacggcg accaattcca ccccggcttc
     2041 tttcaatacc ggggccaaat tgttttcgat gttttcggcg acgcccatgg attcctttaa
     2101 aacaaaaaaa gtggcctcaa ttgccggcca cttctgaact tatactaaca aaacgacggc
     2161 gaaaaggcaa cggtcagatt ttgagggaga gccctccgaa atagctcgcg gaggagtaat
     2221 tgtagcggta aaccgattcg tatttcatgt tggaagaggc gtcttgatag gcgccggtca
     2281 agacggcgtc caggtgttcc atcaaggggt accccaggct gacccggtag gtccaggcgg
     2341 tcgtggttac gttccccgat agatacgtgc cgtcggcgtc ctggacggga cgccgcccgt
     2401 agcgtcgtcg ggaccaagcg gcgcccaggc cgaccgaaag cttttggtta aaacggaggg
     2461 aaacccgggg accggcggtc cattcgacgt aatcgtaata atcgttatta aacaccgtcc
     2521 gtgaagtgtc gtaattgttt tggttggaat tcagccaggc cgcggagaga tccaggcccg
     2581 cgacgctgtc caccgtggcc caccggaact cccgggaagg cccctgccgg cgaagggccg
     2641 cggaggagaa aagaaagaga tcgtgccgca gggt
//
LOCUS       DAOQLS010000244         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22810_length_2672_cov_3.662591, whole genome shotgun sequence.
ACCESSION   DAOQLS010000244 DAOQLS010000000
VERSION     DAOQLS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22810_length_2672_cov_3.662591"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="PK362_05475"
     CDS             complement(<1..1963)
                     /locus_tag="PK362_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC74318.1"
                     /translation="MTPFHLARREIRHRPLHAALFVLSVALGSGVLLGLSGFNAAVET
                     GVQRQARELWAADITVEGTPGFLDDLEVWATGRWPGLRSARTVDTVSMLRAAAGDGVT
                     RATLSGLSPGYPLYGTIGTGSGRSLTDALTAGAVASPKILAQLNLAVGDELVLGRRRV
                     PIADTLAARTDAPASFFEFAPSLLLPLDTLEDSGLLGPGSRSLNKLYLNLPPGVALEP
                     VLEEVKKRAAAETTDVKSWATDNPGVLRFIQNTLTYLNFLGLFLLALAGIGVASALQS
                     AIAASLRSLGMMRALGAPRGFLFRLWGAWIALLLTGGLGGGLALGRLVSFLLARLFGD
                     MIPVSVSLGFPGTALLQTTGLAAAAVGLFALLPLWRLTEISPNSILAWDLPQILPSPR
                     KALMAGIAFAPLFYGLVVLQVRRPGLALEYLLAIAGLILGAALLVAFLSGILRRGLRL
                     SRALTPRLTLRSLARPGSFQTAAGVSLALSFAAVLTLALAQKNLTSQLVDSFPENMPN
                     VFFINVQDAQIPRFRKIVKTDFRLFPLVRGRVVSVNGVPVRDLNARAQQRGDEGDSFT
                     REFGLTYGPDLLPTDTVVAGGALWNDRIDGPQVSGFIEHRRRFGLNVGDRLEFSVLGR
                     RLSATVSSLRSIDQKVRQPFFYFYFKPGPL"
     gene            complement(1960..2643)
                     /locus_tag="PK362_05480"
     CDS             complement(1960..2643)
                     /locus_tag="PK362_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC74319.1"
                     /translation="MTPTAVILEARNLGKSYRVNGRPLPILRDISFQLAAGESVAIQG
                     VSGSGKSTLLSLLAGLDRPTAGEIILENERLDLLPEKELARIRREKIGFVFQAFHLVP
                     SLTVLENVLLPAAFRSGAFSEDRGRALLDRVGLADRAGHFPDQLSGGEKQRAALARAL
                     VNAPKLIFADEPTGNLDSKNGRAVLDLLEEHTRTAGRALVLVTHDDAVASRADRRLRL
                     IDGALAPAP"
BASE COUNT          540 a          815 c          935 g          382 t
ORIGIN      
        1 ccagggggcc cggtttgaag taaaagtaga aaaagggctg tcggactttt tgatcaatgg
       61 aacgcaggga actgaccgtg gccgataagc ggcgtcccag gacgctgaat tccaaccggt
      121 cgccgacgtt caatccgaac cgccgacggt gttcgataaa gcccgacacc tggggtccgt
      181 cgatgcggtc gttccagagg gcgccccccg cgaccaccgt gtccgtcgga agaaggtccg
      241 ggccgtaggt caggccgaat tcccgggtga aggaatctcc ctcgtcgccg cgttgttggg
      301 cccgggcgtt caggtcccgg acgggcacgc cgtttacgga caccacccgg ccgcgaacga
      361 gcgggaaaag gcggaaatcg gttttgacga ttttccggaa ccgggggatc tgggcgtcct
      421 gcacattgat gaaaaagacg ttgggcatgt tttccgggaa agaatccacc agttgagacg
      481 tcagattttt ctgggccagg gcgagggtca acacggccgc gaaggagagg gccaaggaca
      541 cgccggcggc ggtttgaaag gagcccggac gggccagaga ccggagagtc agtcgcggcg
      601 tcagcgcccg ggagaggcgg agcccccgcc gaagaatccc cgataggaaa gcgaccaaaa
      661 gtgccgcgcc caggatcaac cccgcgatgg cgagcaggta ttccagggcc aatcccggcc
      721 gccgcacttg aagaaccacg agcccgtaaa acaacggggc gaacgcgatc cccgccatca
      781 acgctttccg gggcgaagga agaatttgcg gcaggtccca ggccaaaatg gaattgggcg
      841 aaatctccgt caggcgccac agcggaagaa gggcgaaaag cccgacggcc gcggccgcga
      901 gtcccgtggt ctgaagaagg gcggtccccg ggaacccgag ggaaacgctc accgggatca
      961 tgtccccgaa caaccgggcc aggaggaagg aaaccaaccg tcccaaagcc agcccgcccc
     1021 cgaggccccc ggtcaataaa agggcgatcc aggcccccca gaggcggaac agaaatccgc
     1081 ggggagcgcc cagcgcccgc atcatgccca gggatcgaag cgaagcggcg atggccgatt
     1141 gcagggcgct ggccacgccg atgccggcca gggccagaag gaagagcccg aggaaattta
     1201 aataggtgag ggtgttttga atgaaacgaa ggacgcccgg gttgtccgtg gcccaggatt
     1261 ttacgtccgt ggtctcggcc gcggcgcgtt ttttgacttc ttccaaaacg ggttccaggg
     1321 ccacccccgg cgggaggttg agatacagct tgttgaggct tcggctgccg gggcccaaca
     1381 ggccggagtc ttccaaggtg tccaggggca aaagaaggct gggggcgaac tcaaagaagg
     1441 aagccggggc gtcggtgcgg gccgccaggg tgtccgcgat gggcacgcgc cggcggccga
     1501 ggaccaattc gtcgccgacg gcgaggttca attgcgccaa tattttcggc gaagccacgg
     1561 cccccgcggt cagggcgtcg gtcaaggatc gcccggaacc ggtgccgatg gtcccgtaga
     1621 gaggatagcc gggggaaagg cccgacaggg tcgcccgggt cacgccgtcc ccggcggcgg
     1681 ctcggagcat ggagaccgtg tccacggtgc gggccgaccg aagtccgggc cagcggccgg
     1741 tcgcccagac ctcgaggtcg tccaggaaac ccggggtgcc ttcgaccgtg atgtcggcgg
     1801 cccagagttc ccgggcctgc cgttgaacgc cggtttccac cgcggcgttg aaacccgaca
     1861 gccccagcaa cacgccggag cccagagcga cgctcagaac gaagagcgcg gcgtggagcg
     1921 gtcggtggcg aatttcccgt cgggccagat ggaagggggt cacggggccg gcgccagggc
     1981 gccgtcgatc agccgcaggc gtcggtccgc ccgggaagcc accgcgtcgt cgtgggtgac
     2041 gaggacgagg gcccgccccg ccgtccgggt gtgttcctcc aggaggtcca gaaccgcccg
     2101 gccgtttttg gagtccaggt tgccggtcgg ttcgtcggcg aaaatgagct tgggtgcgtt
     2161 gaccagggcc cgggccaggg cggcccgttg tttttccccc ccggacagtt gatccggaaa
     2221 atgcccggcg cggtcggcca ggccgacgcg gtccagcagc gcccgccccc ggtcctccga
     2281 aaaggcgccc gaacggaaag cggccggtaa aagcacgttt tccaacaccg tcaggctggg
     2341 caccagatga aaggcctgga agacgaaacc gattttttcc cggcgaatcc tcgccaattc
     2401 cttttcgggc agaaggtcca ggcgttcgtt ttcaaggatg atttcgccgg cggtgggtcg
     2461 gtccaacccg gccaggaggg aaagcagcgt ggatttgccg ctccccgaaa ccccttggat
     2521 cgcgaccgat tcgcccgcgg ccaattgaaa ggagatgtcg cgaagaattg gcaacgggcg
     2581 cccgttgacc cgataggatt tgcccaggtt ccgggcttcg agaatgacgg cggtcggagt
     2641 catccggcgg tccgataacg cagggccggg aa
//
LOCUS       DAOQLS010000245         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22845_length_2670_cov_3.818738, whole genome shotgun sequence.
ACCESSION   DAOQLS010000245 DAOQLS010000000
VERSION     DAOQLS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22845_length_2670_cov_3.818738"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PK362_05485"
     CDS             <1..965
                     /locus_tag="PK362_05485"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC74320.1"
                     /translation="PGRVALAVILFALANMGFAGGNAFYNAYLPDLGPRESLGAISGF
                     GWAVGYIGGGLCLALDLALIQRPAWFGLPTEHFLPVRASLLSVGLWWGLFGFPIFLFA
                     PAETPGEGRSARAWARLGWSRLLDTARHLARYRNLMIFVLAYALYNDGIETVILTASV
                     VGAELLGLSSGELIQCFLMIQGVAFLGSLLFGALADRWRHKTVILITLAVYTGVILWG
                     ARMTSRGEFWVLGVVLGLVLGGSQAASRSLMGLLTPPERSGEFFGFFGVVGKLTAVLG
                     PLVFGLAAQAGGVRRGVLSLLVFFILGGTVLLFVKEPGTPPPAP"
     gene            complement(901..1653)
                     /locus_tag="PK362_05490"
     CDS             complement(901..1653)
                     /locus_tag="PK362_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNC74321.1"
                     /translation="MKSRRLPSALGRRPLVVASIGDAAHLAVDARRAAKDGADVVEIR
                     ADLFPKSVLKPEPLRRLIKSVREAAHRPLLLTLRIGEEGGGLSPHFREQDRLALFRAA
                     LSDVQGVDIELSANEINRHVEFEAHKRGRFVVVSAHDFGKTPSNAVLSGLARKAKRLG
                     ADVLKVAARPRRRIDVERLMDFCAKSGFRRRVFIAMGPLGEATRRDGFRWGSCLTYGF
                     VRRRLAPGQLPVKALAEASRALLRKAAPSRPE"
     gene            complement(1743..>2670)
                     /locus_tag="PK362_05495"
     CDS             complement(1743..>2670)
                     /locus_tag="PK362_05495"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC74322.1"
                     /translation="IRRARLLAMAMGGLLASAGVVHAAGKAGAVGAAFLKIAPGARAA
                     ALGEAFTAVADDASTLIWNPAGLARLRRPEIAATHSQWLQEADHDFMAAALPVSAGTL
                     GLGVTSFSVPNIPKRASDSDAPDGVFDARDAAYNVGWGQSVGDDWAVGLTASYLRQTI
                     DGRSAAGAAATLGALWRTPWRALTAGLALRNLGGEIKFDREGDPLPTTAALGAALRSP
                     NGRFTFSTDLRLPSKGDLSESAGVEYTHPLFNDGRGRLRAGYNSAAADADGASGLSLG
                     LGLDFSRWAFDVTWAPYGALGDSFRYGFRVSF"
BASE COUNT          438 a          856 c          893 g          483 t
ORIGIN      
        1 cgcccggccg ggtcgccttg gccgtgattt tgttcgccct ggccaacatg ggcttcgccg
       61 gcgggaacgc cttttacaac gcctatctcc ccgacctggg cccccgggaa tccctcgggg
      121 ccatctccgg cttcggctgg gccgtcggat acatcggggg cgggctttgc ctggctctgg
      181 atttggcgtt gattcaacga ccggcttggt tcggactgcc gaccgaacat ttccttccgg
      241 tccgggccag tttgctttcg gtgggcctgt ggtggggtct ttttggattt ccgatttttc
      301 tctttgcccc ggcggaaacc ccgggcgaag gccggtccgc ccgggcctgg gcgcggttgg
      361 gttggtcccg ccttctcgac acggcccgcc acctcgcccg ttaccggaac ctgatgatct
      421 tcgttctcgc ctacgccctt tacaacgacg gcatcgaaac cgtgatcttg acggcctcgg
      481 tggtgggcgc ggaattgctg ggcctttcct cgggcgaact catccaatgc ttcctcatga
      541 ttcaaggcgt ggcctttctg ggctcccttc tttttggtgc cctggccgat cgctggcgcc
      601 acaaaaccgt aatcctgatc accctcgcgg tttacacggg agtgatcctg tggggggccc
      661 gaatgacttc ccggggcgaa ttttgggtct tgggggtcgt tctggggttg gttttggggg
      721 gcagtcaggc ggccagccgg tcgctcatgg gattgctgac gccgccggaa cgcagcggtg
      781 aatttttcgg atttttcggc gtggtgggaa aattgacggc cgttttgggg cctttggtgt
      841 ttggtctggc cgcccaggcg ggcggtgtgc ggcggggcgt gctctcgttg ctggtgtttt
      901 ttattctggg cgggacggtg ctgcttttcg taaaagagcc cgggacgcct ccgccagcgc
      961 cttgacgggc agttgccccg gcgccaatcg tcgacggaca aacccgtagg tcaggcagga
     1021 cccccaccga aaaccgtccc gccgggtggc ttcgcccaag gggcccatgg cgatgaacac
     1081 ccgccgacgg aagcccgact tcgcgcaaaa atccatcaac cgttccacgt cgatccgccg
     1141 gcgggggcgg gccgccacct tcaagacatc ggcccccaat cgtttcgcct tccgggccaa
     1201 ccccgacaac accgcgttgg acggggtttt tccgaaatcg tgggcgctca caacgacgaa
     1261 acggccccgc ttgtgggcct caaattccac gtgacgattg atttcattgg ccgacaactc
     1321 gatgtcgacc ccctgaacgt ccgaaagcgc cgcgcggaaa agcgccaacc ggtcctgttc
     1381 ccggaaatgg ggcgacagcc cgcccccttc ctccccgatt cgcaaggtca aaagaagggg
     1441 ccggtgcgcg gcttcccgga cggatttaat cagtcggcgg agcggctcgg gtttgagaac
     1501 gcttttgggg aaaagatcgg cgcgaatctc gacgacgtcg gccccgtcct tggcggcgcg
     1561 gcgggcgtcg accgcgagat gggcggcgtc cccgatggaa gccaccacga gcgggcggcg
     1621 tcccaaggcg gagggaagtc gtcgcgattt catgagcggg gagaagaaaa cgccgggcgc
     1681 gggtcgatca tggagttgat gatcaggata aatgatcgtc gggtaaaaat caacggtgtt
     1741 ttttagaaag agacccggaa cccgtagcgg aaggagtcgc ccagcgcccc gtagggcgcc
     1801 caggtcacgt cgaaggccca ccggctgaaa tccaaaccca aaccgagcga gaggccggaa
     1861 gcgccgtcgg cgtcggccgc cgccgagttg tagcccgccc gcaaacgtcc ccgaccgtcg
     1921 ttgaacagag ggtgggtgta ttccacaccg gcggattcgg agaggtcgcc cttggaagga
     1981 agccgcagat ccgtggaaaa agtgaatcgg ccgttggggg accgaagcgc cgcgcccaaa
     2041 gccgccgtcg tgggcagcgg gtcgccttcc cgatcgaatt ttatctcccc gcccaggttt
     2101 cgcaaagcga ggccggccgt gagggcacgc cagggcgtgc gccaaagcgc gcccagggtg
     2161 gccgccgcgc cggcggccga acgcccgtcg atggtttgcc gaaggtagga cgccgtgagg
     2221 cccacggccc aatcgtcgcc cacggattgc ccccatccca cgttgtaagc ggcgtcccgg
     2281 gcgtcgaaca cgccgtccgg cgcgtcgctg tccgaggccc gtttggggat gttcgggacc
     2341 gagaaggagg tgaccccgag cccgagggtt cccgcggaaa cgggaagggc cgccgccata
     2401 aaatcgtggt cggcctcttg aagccattgg ctgtgggtgg ccgcgatttc ggggcggcgt
     2461 aggcgggcca agcccgcggg attccaaatc aaggtcgacg cgtcgtcggc cacggcggtg
     2521 aaggcttcgc ccagggccgc ggcccgggcg ccgggagcga tcttcaagaa agcggcgccg
     2581 accgcccccg ccttgcccgc cgcgtggacg acccccgccg acgccaaaag gccccccatc
     2641 gccatcgcca aaagccgcgc tcgccgaatc
//
LOCUS       DAOQLS010000246         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_22879_length_2668_cov_4.209338, whole genome shotgun sequence.
ACCESSION   DAOQLS010000246 DAOQLS010000000
VERSION     DAOQLS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22879_length_2668_cov_4.209338"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PK362_05500"
     CDS             complement(<1..703)
                     /locus_tag="PK362_05500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC74323.1"
                     /translation="MTPLRKATPLAEPLISGGRSLRRLDRFLAGLERQSNGALEMEGV
                     GFLLALGALDGGTGPDVSLLLFYLVPVFWYAWFLGRRHGLLMALAAAVVEFTVDLKSA
                     HAHPPPVIGLWNAAAKAGFFAVAALLLTALRRSFEQERRLAQIDPLTGVLNRRAFVDR
                     LRLEVDRSRRRKSPLTLIYLDVDHFKDINDRWGHPAGDRILKSAVATLRKDFRRTDAV
                     ARLGGDEFAVLLPDTD"
     gene            complement(820..1353)
                     /locus_tag="PK362_05505"
     CDS             complement(820..1353)
                     /locus_tag="PK362_05505"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNC74324.1"
                     /translation="MRRFFLFAFLLVGAVGLRAGGWVLDSSTLTYRVTHRLHKVEGTS
                     HGARGKGLCDGSGCRFLVAAPVNTFDSGDSNRDLHMIETTRGAQFPMVKVSVNLAAVP
                     AGEAFTADLSIEFAGKSHRYASVPFRVADRSAGLRFTGQIPLRLTDFSVPAPSLLGMA
                     VKDDVPVDVEMSWSQKP"
     gene            complement(1363..1950)
                     /locus_tag="PK362_05510"
     CDS             complement(1363..1950)
                     /locus_tag="PK362_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74325.1"
                     /translation="MNCVRLAIAAALIFSSFPVPGFSEESGPSLSLDDLGFKEDQTRG
                     NAELQNQLHRRSRMLKTHQILGLATAVPLIATVMTAPEYESATPGGVNSETKTHRALG
                     VTAGAMYLTTASFAFFAPEGEGVKRNKGLTKVHKALAWIHFPAMIAAPILGYQAYKQA
                     DRGESVHGAAKHHKEVAGLAAGTYTAAMLVMVFNF"
     gene            2021..>2668
                     /locus_tag="PK362_05515"
     CDS             2021..>2668
                     /locus_tag="PK362_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC74326.1"
                     /translation="MTSIIPPPAPPDRTPGFRLPRNVLVLGGISLLNDTASEMIYPLL
                     PIFLTQTLGASAASLGLIEGVAESAVSLFKLFSGYLSDRLHRRKIWIGVGYAVSNAVR
                     PLIALAGSWPAVLALRFADRIGKGLRTSPRDALVAESVPPEHRGRAFGFHRAMDHLGA
                     VIGPLLAALILWRLPDRLRLIFFLSAIPGALVVLLVARGLREIPGTVRDSRVFQPL"
BASE COUNT          504 a          839 c          841 g          484 t
ORIGIN      
        1 cgtcggtgtc gggcagcaac accgcgaatt cgtcgccgcc cagtcgggcc acggcgtcgg
       61 tccggcggaa atctttccgc agggtggcca cggcggattt cagaatccga tcgccggcgg
      121 gatggcccca acgatcgttg atgtccttaa aatggtccac gtccaggtag atcaacgtca
      181 gcgggctttt ccgtcgacgg gaacggtcca cctccagccg aaggcggtcg acgaaagccc
      241 ggcggttgag gacaccggtc aagggatcga tctgggccag ccggcgctcc tgttcaaaag
      301 agcggcggag ggcggtgagc aggagggcgg ccacggcaaa aaacccggct ttggcggcgg
      361 cgttccataa accgatgacc ggagggggat gggcgtgggc cgatttaaga tcgacggtaa
      421 attccaccac ggccgcggcc agggccatga gcaagccgtg acgacggccc aaaaaccagg
      481 cgtaccaaaa aacggggacc aggtagaata ggagcaggga aacgtcgggg cccgttcccc
      541 cgtcgagcgc gccgagggcc aggaggaatc cgacgccttc catttcgagc gccccgttcg
      601 attggcgctc caaacccgcc aaaaatcggt ccagccgacg gagggaccgc ccccccgaaa
      661 tcaggggttc ggccaggggc gtggccttgc gaaggggggt cacggcggta tcgtctcaag
      721 tccggcgacg gccgtcaaga gacggggttt cgttctttcg gtcccggccc ccgcgtcgac
      781 gcgtcgattc ccgggccgtc gggagggccc ccgaatcgat taaggttttt gggaccagct
      841 catttcgaca tcgacgggca cgtcgtcctt gaccgccatc ccgagcaaag acggggccgg
      901 aacgctgaaa tccgtcaggc gaagagggat ttgaccggtg aatcgaaggc cggccgaacg
      961 atccgccacg cgaaaaggaa cggacgcgta gcggtgggat ttcccggcga attcgatgga
     1021 caggtccgcg gtgaacgcct cccccgcggg gacggccgcc aagttgaccg acactttgac
     1081 catgggaaat tgggcccccc gggtcgtttc gatcatgtgc aggtcgcggt tgctgtctcc
     1141 ggaatcaaag gtattgacgg gagcggccac cagaaaccgg caccccgaac catcgcagag
     1201 ccctttgccc cgggccccgt ggctggtgcc ttccactttg tgcaaacggt gggtcacgcg
     1261 gtaggtcagg gtcgatgaat ccaaaaccca ccccccggcc cgaaggccga ccgcgcccac
     1321 caatagaaaa gcgaagagga aaaatcggcg cacgggagcc tcctagaagt taaagaccat
     1381 gaccaacatg gccgcggtgt aagttccggc ggccaacccg gccacttcct tgtggtgctt
     1441 ggccgccccg tgaacgcttt ccccccgatc ggcctgcttg taggcctggt agccgagaat
     1501 gggggcggcg atcatggcgg gaaaatgaat ccaagccagg gccttgtgga ctttggtcag
     1561 ccccttgttc cgtttcaccc cttcgccctc cggggcgaaa aacgcgaagg acgccgtcgt
     1621 cagatacatg gcccccgccg tgacgcccaa ggcgcggtgg gtcttcgttt ccgaattgac
     1681 gccccccggg gtcgccgatt cgtattccgg ggcggtcatc accgtggcga tcaagggcac
     1741 ggccgtggcc agtcccagaa tttgatgggt ttttaacatg cgcgatcgac gatgcaattg
     1801 attttgaagt tcggcgttgc cgcgggtttg atcctcttta aaacccaggt cgtccaagga
     1861 cagggacgga ccgctctcct cggagaaacc cggaacgggg aaagacgaaa aaatcaacgc
     1921 ggcggcgatg gccagacgaa cgcaattcat agagaacctc cgacaggatg ggaaacacct
     1981 tccgatgcgt ttcattatag taatcttgtt ttttaataac atgacatcca tcataccgcc
     2041 ccccgctccc ccggaccgaa cgcccgggtt ccgactgccg cgcaacgttc ttgttctcgg
     2101 cgggatcagc cttctaaacg acaccgcttc ggaaatgatt taccccctcc ttccgatttt
     2161 cctcacgcaa accctcgggg cgtcggcggc gtccctgggc ttgatcgaag gggtcgccga
     2221 atcggccgtg tccctgttca aacttttttc gggctatctg tcggaccgcc ttcaccgtcg
     2281 gaagatctgg atcggcgtgg ggtacgccgt ctcgaacgcc gtgcgacccc tgatcgccct
     2341 ggcgggatcc tggccggcgg tgctggccct tcgtttcgcc gatcggatcg gcaaagggct
     2401 tcgcacctcc ccccgggacg ccctggtggc ggaatccgtt ccaccggaac accgggggcg
     2461 ggccttcgga tttcaccggg ccatggacca tctcggggcc gtgatcgggc cccttctggc
     2521 ggccttgatc ctctggcggt tgccggatcg cctgcggctt attttcttcc tatcggcgat
     2581 tccgggcgcc ttggtcgtcc ttctggtcgc ccgaggtctg cgggaaatcc ccggaacggt
     2641 ccgggattcc cgcgttttcc agcccctg
//
LOCUS       DAOQLS010000247         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23103_length_2655_cov_4.018462, whole genome shotgun sequence.
ACCESSION   DAOQLS010000247 DAOQLS010000000
VERSION     DAOQLS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23103_length_2655_cov_4.018462"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PK362_05520"
     CDS             <1..750
                     /locus_tag="PK362_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNC74327.1"
                     /translation="TGPTGSGKTTTLAAILDHINSTQAVHIITLEDPIEYVYEDKKAV
                     INQREVGVDTHDFSQGLRRALRQDPDVILIGELRDAETMTTAMNAAETGHLVLGTLHT
                     NDAKQSIDRIIDTFPPDQHNQIRMQLAKCLLGVSAQRLIKKADGKGRIALQEILINSP
                     TIEKLIEDNKIGAIGKAIEDSAEFYKMQTFNQALFRMIQKHMVEIEEAMSYSPNPNDL
                     KIKLQSEGLLTQHAEIPVDLPPGLRGGGRAG"
     gene            752..1720
                     /locus_tag="PK362_05525"
     CDS             752..1720
                     /locus_tag="PK362_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC74328.1"
                     /translation="MVPVDRLKDLPLFAGLPDPVRALLAERLESRSVKSGDTLFAEGD
                     PGDAVYFVLSGRVAIQKTLDAAKGTFKTLAVLGAGDFFGEMALLEKEDRSAAAVAAAD
                     GEVLSLSAKDFGRWLADDSKIPLKFLLPFVTTLSARLRDTTREMAAFFEVSRVLVQNL
                     EIKDMAGRLLDVMAQPFNGEIQGAFFVWDPFGSEYEGVAAVGGWPERLRTARPSTDPL
                     FYWMAEKGECLLAPDWESDARFIDAVRAQWPAFRSVLAAPVPGDKRPAGFMVLGHNGE
                     RGFFTAGHRRTLASVANLIAPAFENAALHEERRAEERLRRARLDRR"
     gene            1755..2180
                     /gene="rsfS"
                     /locus_tag="PK362_05530"
     CDS             1755..2180
                     /gene="rsfS"
                     /locus_tag="PK362_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNC74329.1"
                     /translation="MTSTASARRFLAAARLAARGADDKKARDIRLWDVRRLSGVADFY
                     VVATCDSNPQIQAAEDAIEESLKGKGLRPLRREGRGSAPWRVLDFGGLVVHLLTESAR
                     LFYGLERLWEGAKSMPWAERAARASARPSRSRRAPNPTR"
     gene            2206..>2655
                     /gene="argS"
                     /locus_tag="PK362_05535"
     CDS             2206..>2655
                     /gene="argS"
                     /locus_tag="PK362_05535"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNC74330.1"
                     /translation="MKEIVRNALLGACRQWAAEQPEPFPVDAAALSVDAPPPHVPGDL
                     ASQWPLAAAKRAKKNPRQVAQEILAKIPTGDLFEKTEIAGPGFLNFSLSAGWLLAALR
                     GLLTAGAAYGRRPAHTGQKVLIEFVSANPNGPLHVGHGRGAALGDSLS"
BASE COUNT          474 a          900 c          835 g          446 t
ORIGIN      
        1 acgggcccca cgggctcggg caagacgacg acgctggccg ccattttgga ccacatcaac
       61 agcacccagg ccgtccacat catcaccctg gaagacccca tcgaatacgt ctacgaggat
      121 aaaaaggccg tcatcaacca gcgggaagtg ggggtggaca cccacgactt ttcccaggga
      181 ctccgccgcg ccctgcgcca ggaccccgac gtgattttga tcggcgagct ccgggacgcc
      241 gaaaccatga cgaccgccat gaacgcggcc gaaaccggcc atttggtgct cggcaccctt
      301 cacaccaacg acgccaaaca gtccatcgac cggatcatcg acacctttcc gccggatcag
      361 cacaatcaga tccgcatgca gctggccaag tgcttgttgg gcgtgtccgc tcagcgcctc
      421 attaagaaag ccgacggaaa aggccgcatc gccctgcagg aaatcctgat caattcgccg
      481 acgatcgaga aattgatcga ggacaacaaa atcggcgcca tcggcaaggc catcgaggac
      541 tcggcggagt tttacaagat gcagaccttc aaccaagccc tcttccgaat gattcaaaaa
      601 cacatggtcg aaatcgagga ggccatgagc tactccccga accccaacga cttgaaaatc
      661 aaattgcaat cggaaggcct tctcacccag cacgccgaaa tccccgtgga cctgcccccg
      721 gggttgcgcg gcggggggcg ggcgggctga cgtggttccc gtcgaccgcc tgaaggacct
      781 tccgctgttc gcgggcctgc cggaccccgt tcgcgcgctc ctggcggagc gattggagtc
      841 gcggtccgtg aaatccggcg acaccctttt cgccgaaggc gatcccggcg acgcggttta
      901 tttcgtcctg tccgggcggg tggccattca gaaaaccctg gacgcggcca aaggcacctt
      961 taaaaccttg gcggtcctcg gcgcgggcga tttcttcggc gaaatggcgc tgttggagaa
     1021 ggaagaccgg tcggcggcgg cggtggcggc cgccgacggc gaagtgctct ccctctcggc
     1081 caaggacttc ggccgctggc tggccgacga ctcgaagatc cccctgaaat tcctgctccc
     1141 ctttgtgacc accttgagcg cgcgcctgcg cgacacgacc cgggaaatgg ccgccttctt
     1201 tgaagtgagc cgggtcttgg ttcagaatct ggaaatcaag gacatggcgg ggcgcctgtt
     1261 ggacgtcatg gcccaaccgt tcaacggcga aatccagggg gcgttttttg tctgggaccc
     1321 cttcgggagc gaatacgagg gcgtcgccgc ggtgggcggc tggccggagc ggttgcggac
     1381 ggcccgccct tcgacggacc ccctgtttta ctggatggcg gaaaagggcg agtgcctgct
     1441 cgcgcccgat tgggaatccg acgcccgctt catcgacgcg gtgcgagccc agtggccggc
     1501 ctttcgatcg gtgttggccg cgccggtccc gggggacaag cgtccggcgg gattcatggt
     1561 gttgggccac aacggcgaac ggggtttttt caccgcgggg caccgtcgca ccctggccag
     1621 cgtggccaat ttgatcgcgc cggccttcga gaacgccgct ttgcacgaag agcgccgggc
     1681 cgaggaacgc cttcgccggg cccggctgga ccgccgttga agggcccgtc gattccccga
     1741 aagccggcct cccgttgact tccacggcct ccgcccggcg gtttttggcc gccgctcgac
     1801 tggccgcccg gggcgcggac gataaaaaag cccgggacat ccgcctgtgg gacgttcgcc
     1861 gcctgtccgg cgtcgccgat ttttacgtcg tggccacctg cgattccaac ccccagatcc
     1921 aagccgccga agacgccatt gaagagtcgt tgaaaggaaa gggactgcgt cccctgcgcc
     1981 gggaaggccg ggggtcggcc ccctggcggg tcctggattt cggcggcctg gtggtccatc
     2041 tgttgacgga atccgcccgg ctgttttacg gattggagcg tttgtgggag ggggccaaat
     2101 cgatgccctg ggcggagcgc gccgcccgag cgtccgcccg gccgtcccgc tcccgccgcg
     2161 cgccgaaccc aacccgctga ccttctgcta caatagcggc cctccatgaa agaaatcgtc
     2221 cggaacgccc tgttgggcgc ctgccgccaa tgggcggccg agcagcccga acctttcccc
     2281 gtggacgcgg cggccctttc ggtcgacgcc ccgccgcccc acgttcccgg ggatttggcc
     2341 tcccagtggc ccctcgcggc ggccaagcgg gccaagaaaa acccccgcca ggtggcccag
     2401 gaaatcctcg ccaaaatccc gacaggggat ttgttcgaaa aaacggaaat cgccgggccg
     2461 ggttttctca atttttccct gagcgccggt tggttgctgg ccgcgttgcg cggtctcctg
     2521 accgccgggg ccgcctacgg ccgccgcccc gcccacaccg gtcaaaaagt cctgatcgaa
     2581 tttgtgagcg ccaacccgaa cgggcccctg cacgtgggcc acggccgcgg cgccgccctg
     2641 ggggactccc tttcc
//
LOCUS       DAOQLS010000248         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23176_length_2650_cov_4.495183, whole genome shotgun sequence.
ACCESSION   DAOQLS010000248 DAOQLS010000000
VERSION     DAOQLS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23176_length_2650_cov_4.495183"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..710
                     /locus_tag="PK362_05540"
     CDS             72..710
                     /locus_tag="PK362_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC74331.1"
                     /translation="MLSLGLGSSVVTVLEMTSAIGTLANAGVHMQPFAVLKVVDRNGK
                     VLEEHVPQGEPAISPQSAFLTTRLMQAVVQEGTGAYARNVGRPVAGKTGTTQDMRDFW
                     FMGFGPDLVTGVWLGYDDFVPLGKKLTSAGTTVPWWTDYMAQAVKFLPTRDFPVPPGV
                     SFAKIDRDTGYLALPTCPHVVLEAFRDGAAPKEFCPVDHEAQEDLKDENITE"
     gene            707..2392
                     /locus_tag="PK362_05545"
     CDS             707..2392
                     /locus_tag="PK362_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNC74332.1"
                     /translation="MTPSPTPAEFSLLTDIALGIVFAAAASHVARLLRQPLILGYVLG
                     GVLLGSHLGFGLVTDERSIELISEIGLIFLLFIIGLEINLRELAKMGKAALAVGGLQM
                     AGGVALGGLFFGSLRSVFGVGSFEVLYLAVAAALSSTLIVVKLLHDKFELHTTSGRLT
                     VGVLVLQDLAAILFLALQPNLSHPQLVQIASSLSLGLALVGVSFLVSRFLLSPLFSAA
                     ARLPELVLLTSIAWCFGLGAAAEHLGLSKEMGALIAGLSIAAFPYGADVISKLSGVRD
                     FFVTLFFVSLGLKVAPPTAHTLGLSGLMILFVFASRLLTVAPTAAIMKQGLRNGLLVG
                     INLSQISEFSLVILALGTGYGHVSSELSALVLTALLLASVVSTYAIQFNDKLVRGLLA
                     LLGVVGIRERSTHHPSEAQLLKEHRDIVLLGCYRIGQGFLSAVEKSAPSLKKRILVVD
                     YDATQKDRLRARGFHWLYGDLANPETLRHIGIEQASLVICSISDTFLKGITNRRLLSH
                     LKDLSPKAKIVMTADENEEADRMLKEGAGDVMVSGRLSGAYLFDLVTGKKPLA"
     gene            complement(2389..>2650)
                     /locus_tag="PK362_05550"
     CDS             complement(2389..>2650)
                     /locus_tag="PK362_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC74333.1"
                     /translation="TRFLACRSLITALVRALKGKKIFAAGLDVFEKEPQLAPGLAELP
                     NVLLAPHAGSATVETRAEMSVLAARNVLAVLEGRPALTPVGI"
BASE COUNT          424 a          907 c          819 g          500 t
ORIGIN      
        1 gaccgcattc tcggcgagga tgtcggccat ggcgacctga ccaccgcggc actcaaagcc
       61 ccctggaccc ggtgctgtcc ctggggctgg gctcgtcggt ggtcaccgtc cttgaaatga
      121 cgagcgccat cggcaccctg gccaacgcgg gcgtgcacat gcagcccttc gcggtgctca
      181 aagtggtgga ccgcaacgga aaagttctgg aggaacacgt gccccagggc gagccggcca
      241 tctcccccca gagcgccttt ttgaccacgc ggctcatgca ggccgtggtg caggaaggca
      301 ccggcgccta cgcccggaac gtcggccgcc ccgtggccgg caaaaccggg accacccagg
      361 acatgcgcga cttttggttc atgggcttcg gccccgacct ggtgaccggc gtgtggctgg
      421 gctacgacga ctttgtgccc ctgggcaaga aactgaccag cgccggaacc acggtgccct
      481 ggtggacgga ctacatggct caggccgtga agttcctgcc gacccgggac ttccccgttc
      541 cccccggcgt gtccttcgcc aaaatcgacc gggacacggg ctacctggcg ctccccacct
      601 gcccccacgt ggtgctggaa gccttccgcg acggcgcggc ccccaaggag ttctgccccg
      661 tggaccacga ggcccaggaa gatctgaagg acgaaaacat caccgagtga cgccttcccc
      721 gaccccggcg gaattttccc tcctcaccga catcgccctc ggcatcgttt tcgccgcggc
      781 ggcctcccac gtggcgcggc tgctccgcca accgctcatt ctgggctacg tgctgggcgg
      841 ggtgctgttg ggttcccacc tgggcttcgg cctcgtcacc gacgagcgga gcatcgagct
      901 catctccgaa atcgggctca tcttcctcct cttcatcatc ggcctggaaa tcaaccttcg
      961 ggaattggcc aaaatgggca aggccgccct ggccgtgggc gggcttcaaa tggccggggg
     1021 cgtggccctg ggcgggctgt tcttcggttc gctccggtcg gtcttcggcg tggggtcctt
     1081 cgaggtgctg tatctggccg tggccgcggc gctcagctcc accctgatcg tcgtcaaact
     1141 tctccacgac aaattcgaac tgcacacgac ctccggccgc ctcacggtgg gcgtgctggt
     1201 gctccaggac ctggcggcca ttttgttcct ggcccttcag cccaacctgt cccacccgca
     1261 gctggtgcaa atcgcctcgt ccttgagcct gggcctggcg ctggtgggcg tgtccttcct
     1321 ggtgagccgc ttcctcttaa gtccgctttt ctccgccgcc gcgcggctgc cggagttggt
     1381 gcttctcacc tccatcgcct ggtgcttcgg cctgggggcc gcggcggaac acctggggct
     1441 ctccaaggaa atgggcgccc tgatcgcggg cctgagcatc gcggccttcc cctacggcgc
     1501 cgacgtgatt tccaaacttt ccggcgtgcg ggactttttc gtgaccctct tcttcgtttc
     1561 cctgggcctc aaggtggcgc ccccgacggc ccacaccctg gggctctcgg ggctcatgat
     1621 cctgttcgtc ttcgccagcc gcctcctgac cgtggcgccc acggcggcga tcatgaagca
     1681 ggggttgcgg aacgggctcc tcgtcgggat caacctctcc cagatcagcg agttctccct
     1741 ggtcatcctg gccctgggca cgggctacgg ccacgtgtcg tcggagttgt cggccctggt
     1801 gttgacggcg ctcctcctgg cgtcggtggt ctcgacctac gccattcagt tcaacgacaa
     1861 attggtccgg ggccttctgg ccctcctggg ggtcgtggga attcgggagc ggtccacgca
     1921 ccacccgtcg gaagcgcaat tgctgaagga acaccgggac atcgtgctcc tgggctgtta
     1981 ccggatcggc cagggatttt tgagcgcggt ggaaaagtcg gccccgagcc ttaaaaaaag
     2041 aatcctggtg gtggattacg acgccacaca aaaggaccgc cttcgggccc ggggcttcca
     2101 ttggctttac ggcgacctgg cgaacccgga aaccctccgt cacatcggga tcgaacaggc
     2161 cagcctcgtg atttgttcca tctccgacac ttttctcaag ggcatcacca accggcgcct
     2221 gctctcgcac ttgaaggacc tcagtcccaa ggccaaaatc gtcatgaccg ccgacgagaa
     2281 cgaggaggcg gaccggatgc tcaaggaagg ggcgggggac gtgatggtct ccggacggct
     2341 gtccggagcg tatttgttcg atttggtgac ggggaaaaaa ccgctggcct aaattccgac
     2401 cggcgtgagg gccgggcggc cctccaaaac ggccagcacg ttccgggccg ccaaaaccga
     2461 catctcggcc cgggtttcaa cggtggcgct tccggcgtgg ggcgccaaca aaacgttcgg
     2521 caattccgcc aaccccggcg ccagttgggg ttccttctcg aaaacgtcca accccgcggc
     2581 gaaaatcttt ttccccttca gcgcccgcac cagggccgta atcaggctcc ggcaggccag
     2641 aaaacgcgtg
//
LOCUS       DAOQLS010000249         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23182_length_2650_cov_3.943738, whole genome shotgun sequence.
ACCESSION   DAOQLS010000249 DAOQLS010000000
VERSION     DAOQLS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23182_length_2650_cov_3.943738"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PK362_05555"
     CDS             <1..731
                     /locus_tag="PK362_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC74334.1"
                     /translation="WYIELAKPRTQGSDETSARAARQTLAEVLDGILRALHPIMPFLT
                     EELWQALNDTLGEKPAESLMIAVEKPSAVGDPASDADRRAMDLLQGAVTALRTIRSEM
                     NVPPGKPIALVVNATGASAATQDTLARHGGYLRHLAKIGEWKMIDSGPCPRPPQSASA
                     VAADFEMYLPLEGLIDFGKEKERLEKEKTALEADLARLKERLENPDFIARAPADKVEE
                     VRTRLAESAAKRERVHGHLAALSA"
     gene            complement(728..1546)
                     /locus_tag="PK362_05560"
     CDS             complement(728..1546)
                     /locus_tag="PK362_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNC74335.1"
                     /translation="MTGALAVYRKSNFAPAFWCLAPRRRRALSAVYAFARAVDDAVDE
                     VGIEGKDPAEARRILNAWREALRSDTAPRGIDGSLWPFLRDAVTIFRVDRRHLLELVD
                     GVERDLTQTRYANAVDVDEYCYGVAGTVGLACLPIGLDAVKHRDFAVVLGRAVQWVNI
                     LRDVKTDALRGRIYLPAADLRRFGVGEEEILGLVHSERLRDLLAFEADRAGSFFDEAE
                     RALPAADRGAARPARVMGRLYRRLLDKMRAGEFRVFAERPRLSVAEKIICLVGG"
     gene            complement(1543..2166)
                     /locus_tag="PK362_05565"
     CDS             complement(1543..2166)
                     /locus_tag="PK362_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNC74336.1"
                     /translation="MACNTSTAVALRFLRARYDLPVDPFDRLITAFERDVAVRRHPTF
                     DDLLFYCRHSANPVGELVLRLHRAWTPERGRWSDALCTALQLANFWQDVAVDARKDRI
                     YFPLEDLRAAGVTEDNVLRGPATPALRGLLEKQVRRTWDFFQAGRPLLADAPPGLRRE
                     LRLVWLGGTAILQKIEARRFDTWSVRPALSPWDWLRLGIRWWRGEPR"
     gene            2341..2650
                     /locus_tag="PK362_05570"
                     /pseudo
     CDS             2341..2650
                     /locus_tag="PK362_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298724.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
BASE COUNT          520 a          929 c          852 g          349 t
ORIGIN      
        1 attggtacat cgaactggcc aagccgcgga cccagggatc cgacgaaacg tcggcccggg
       61 ccgcccgcca aaccctggcg gaagtgttgg acgggatttt gcgggccctg cacccgatca
      121 tgccctttct caccgaagaa ctctggcagg ccttgaacga caccctgggc gaaaaaccgg
      181 cggagtccct gatgatcgcg gtggaaaagc catcggccgt cggcgacccg gcttccgacg
      241 cggaccgtcg ggccatggac ctccttcagg gggcggtgac ggccctgcgc acgatccggt
      301 cggaaatgaa cgtgcccccc ggaaaaccca tcgccctcgt ggtcaacgcc accggggcct
      361 ccgccgccac ccaggacacc ctcgcccggc acggcggcta cctgcgccac ctggccaaaa
      421 tcggcgagtg gaaaatgatc gacagcggtc cctgcccgcg gcccccccag tcggcctccg
      481 ccgtggcggc cgattttgaa atgtaccttc ccttggaagg gctgatcgat ttcggaaaag
      541 aaaaggaacg gctggaaaaa gaaaaaaccg cgctggaggc ggacctggcg cggctcaagg
      601 agcggctgga aaaccccgat tttatcgccc gcgcccccgc cgacaaggtg gaagaagtcc
      661 gaacccgcct ggcggaaagc gccgccaaac gcgaacgcgt gcacggccac ctggccgctc
      721 tgagcgccta accccccacc aagcaaataa ttttttcggc cacggagagc cgcggccgtt
      781 ccgcgaaaac ccgaaattcc cccgcccgca ttttgtccag caaccgccgg tagaggcgcc
      841 ccatgacccg ggccggacgg gccgcgccgc gatcggccgc cggcaaggcg cgctcggctt
      901 cgtcgaaaaa cgacccggcg cgatcggctt cgaacgcgag caaatcccgc aggcgctcgc
      961 tgtgaaccaa acccagaatt tcctcctccc ccaccccgaa ccggcgcaaa tccgccgccg
     1021 gcaggtagat ccgcccccga agggcgtcgg tcttaacgtc ccgcaaaata ttgacccatt
     1081 ggaccgcccg tcccagaacc acggcgaaat cccggtgctt gaccgcgtcc aaaccgatcg
     1141 gaaggcaggc gaggcccacc gttcccgcca cgccgtaaca gtattcgtcg acgtcgaccg
     1201 cgttggcgta gcgggtttgc gtcaaatccc gctcgacccc gtccaccagc tccagcaagt
     1261 gccgtcgatc cacccgaaaa atcgtcacgg cgtcccgcag gaacggccac aaggacccgt
     1321 cgatgccccg gggcgcggtg tccgatcgga gggcctcccg ccaggcgttc aaaattcggc
     1381 gcgcttccgc cgggtccttc ccctcgatcc ccacctcgtc cacggcgtcg tccaccgccc
     1441 gggcgaaggc gtacacggcc gacagggccc gacggcggcg cggggccaag caccagaagg
     1501 ccggggcgaa attgctcttg cgatacaccg cgagggcgcc cgtcaccggg gctccccccg
     1561 ccaccaacgg atccccaaac gcaaccagtc ccagggggac agggcgggcc ggacgctcca
     1621 ggtgtcgaaa cggcgcgcct cgatcttttg aagaatcgcc gttcccccca accacacgag
     1681 gcgcagctcc cgacgaaggc ccgggggcgc gtcggccagg agggggcgtc ccgcttggaa
     1741 aaagtcccag gtgcgtcgaa cctgcttttc caacaagccc cgaagcgcgg gcgtggccgg
     1801 accgcggagg acgttgtcct ccgtcacgcc cgccgcgcgc aggtcctcca ggggaaaata
     1861 aatccggtct ttccgagcgt ccacggcgac atcctgccaa aaattggcca attgcagggc
     1921 ggtgcaaagg gcgtcggacc agcggccccg ctcgggggtc caagcccggt gtaggcgcag
     1981 gaccagttcc cccacggggt tcgccgaatg gcggcaataa aaaagcaggt cgtcgaaggt
     2041 cgggtggcgg cggacggcga cgtcgcgctc gaaagcggtg atcaaacggt cgaagggatc
     2101 cacgggcagg tcgtagcggg cccggaggaa gcggagggcg accgccgtgg aggtgttgca
     2161 cgccaccacg agcgccttca cgtcgcgctt ggcgagctcg tcgagggcct gcgcagagaa
     2221 ccgcagcacc tcgtcgtcgg tgcgcgttcc gtacggggcc cgcaggttgt cgccgaggta
     2281 gatcgtggac tcgtggggcg atcgccggag gatctcgcgc aggaccgtca gcccgccgac
     2341 gccaagttca agcagttcaa ctggaaggtc gtcgaagagc tgaagatgcc gggcgcgggc
     2401 aacctgttcg tgaagacgca ccccaagagc aagaacctct gggccgacct gccgatgaac
     2461 cccgagcgcg agaacgccga gtcggtgtac gtgtacagcc tggccgacct gtccaaggcg
     2521 ccggtgaagc tggacgtggc caaggacagc ggcctgccgc agaccaaggc gctgcgccgc
     2581 gccgtgcacc ccgagtacaa ccaggcgggt gacgaggtct ggatcagcct gtggggcggc
     2641 aagaccgacc
//
LOCUS       DAOQLS010000250         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23240_length_2646_cov_4.628329, whole genome shotgun sequence.
ACCESSION   DAOQLS010000250 DAOQLS010000000
VERSION     DAOQLS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23240_length_2646_cov_4.628329"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /gene="cgtA"
                     /locus_tag="PK362_05575"
     CDS             <1..407
                     /gene="cgtA"
                     /locus_tag="PK362_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HNC74337.1"
                     /translation="RFRVWGREVERLMVVTHFDQDEAVARFQKILKKMGVEDELRRRG
                     AVAGDVVTVGEYEFTYRPDVGDRPVTAGRSAPRRPSERTKDVPSRSFSESKSRGRTAP
                     PRVPFGDAKDYRRAERIGGTRRRSRTSGKMKK"
     gene            418..1524
                     /gene="proB"
                     /locus_tag="PK362_05580"
     CDS             418..1524
                     /gene="proB"
                     /locus_tag="PK362_05580"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNC74338.1"
                     /translation="MALTSRQRLVVKVGTAILSRAEGGLNRARLRQLAEELSGLQKKN
                     FAPLLVTSGAIGAGMDAFGWRVRPTALRDKQAAAAVGQVALMEAYKAAFAEFGVPVAQ
                     ILLTRSDLEDRERYLNIRHTLTALLERGVLPVLNENDTVATDEIKFGDNDSLSALVAA
                     KMDAGHLFILTDVDGLLTSTGAEGQLLPEVFQITPDIEALVRAGTGSSKSVGGMASKL
                     SAARLAMASGVEVWIASGRKPGIVRDILEGHGRGTRFAPQEEALSARKRWIAFGRRVK
                     GALHVDAGAAQAVVDGKRSLLPAGIARVEGAFSAGDTVKILNAAGREIARGLAAHGSG
                     DLQKIKGRRSSEIESVLGHPAPAEVVHRDNLIVL"
     gene            1625..2473
                     /locus_tag="PK362_05585"
     CDS             1625..2473
                     /locus_tag="PK362_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC74339.1"
                     /translation="MKKWVSGLFAVFFLAGCTAHRIFYFPDSKLYVDPDKLGLAYEVV
                     TYPSLNGKQLVGVYFKAEGPAVGTVVHCHGNAHNVSDHFPLALFLRKGGFDVLCFDYQ
                     GFGASEGRPSPEGTVEDAVASVRYASARLAPGARGVVLFGQSLGVAVAVGAAVREPSV
                     KALVLEGGFNSYRVITRTVLRRSWILWPVSAILPPILVRKTWDPDKIVAEISPRPLLF
                     IHGTADRVVPAVLTERLFARARDPKELWLIPGAGHLQCHRVAGAAYEEKIIGFYRRAL
                     GLTENN"
     gene            complement(2457..>2646)
                     /locus_tag="PK362_05590"
     CDS             complement(2457..>2646)
                     /locus_tag="PK362_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74340.1"
                     /translation="SYSPLKISAEGGVIRYGWGQVDTVTRWNLTAGWSLSPRWLWRTE
                     ISRTGIYREAATSLNYFL"
BASE COUNT          456 a          903 c          825 g          462 t
ORIGIN      
        1 accgcttccg cgtctggggc cgcgaggtgg agcgcctcat ggtcgtgacc catttcgacc
       61 aggacgaggc cgtggcccgt tttcaaaaga ttctcaaaaa aatgggcgtc gaagacgaat
      121 tgcgccgccg gggcgccgtg gccggggacg tcgtgaccgt cggcgagtac gagttcactt
      181 accggcccga cgtgggcgac cgccccgtga ccgccggccg ctcggccccc cgccgaccct
      241 cggaacggac caaggacgtg cccagccgct ccttcagcga atccaagagc cggggccgga
      301 ccgccccgcc ccgggtgccc ttcggcgacg ccaaggatta ccgacgggcc gagcgcatcg
      361 ggggaacccg gcgccgttcc cggacctcgg gaaaaatgaa aaaataaagg ggcgcccatg
      421 gccctcacgt cccgtcaacg gttggtggtc aaagtcggca cggcgatctt aagccgcgcc
      481 gagggcggcc tgaaccgcgc ccgcctccgt caattggccg aggaactctc cggtctccaa
      541 aagaaaaatt tcgcccctct cctcgtgacc tccggcgcca tcggcgccgg catggacgct
      601 ttcggctggc gcgtgcgccc caccgccctt cgggacaaac aagccgccgc ggcggtgggc
      661 caggtggcct tgatggaagc ctacaaggcc gccttcgccg agttcggcgt ccccgtcgcc
      721 caaatcctcc tgacccgctc ggacctggag gaccgggaac gctacctcaa cattcgccac
      781 accctcaccg cccttctgga acggggggtg ctgcccgttt tgaacgaaaa cgacaccgtg
      841 gccaccgacg agatcaagtt cggcgacaac gactcgctct cggccttggt ggcggccaaa
      901 atggacgcgg ggcatctctt cattttgacc gacgtggacg gcctcctcac cagcaccggg
      961 gccgaaggcc aacttctgcc cgaagttttt caaatcaccc ccgacattga ggccctggtc
     1021 cgggccggaa ccggctcgtc caaaagcgtg ggcggcatgg cctccaagct ctccgccgcg
     1081 cggttggcca tggcctcggg cgtggaagtg tggatcgcct cgggccgtaa acccgggatc
     1141 gtccgggaca ttttggaagg ccacggccgg ggcacccggt tcgcccctca ggaagaagcc
     1201 ctgagcgccc gcaaacgttg gatcgccttc ggccgccggg tcaagggcgc cctgcacgtg
     1261 gacgcgggcg ccgcccaagc tgtggtggac ggcaagcgaa gtctcctgcc cgcgggcatc
     1321 gcccgggtgg agggcgcctt ttccgcgggg gatacggtga aaatcctaaa cgccgccggc
     1381 cgggaaatcg cccggggttt ggcggcccac gggtccgggg acttgcaaaa aatcaaaggc
     1441 cggcggtcct ccgaaatcga gtcggtcctg ggccatcccg cccccgccga agtcgttcac
     1501 cgggacaacc tcatcgtcct ctaggcgact ccccgccggc ggccatcgtc aaacgatgcg
     1561 ccatctcaac acgttctttc gcccttcgcc gaccccgccc attaattttg ttaccattcc
     1621 ttcgatgaaa aaatgggtct cgggactctt cgcggttttt ttcctcgccg gctgcaccgc
     1681 ccacagaatc ttttattttc ccgattcaaa actctacgtg gacccggaca aattgggcct
     1741 ggcctacgag gtggtgacct accccagcct caacggcaaa caattggtgg gcgtctattt
     1801 taaggcggag ggaccggccg tcggcaccgt ggtgcattgc cacgggaacg cccacaacgt
     1861 gtcggaccat tttcccctgg ccctcttttt gcgaaaaggc gggttcgacg ttttgtgttt
     1921 tgactatcag ggattcggcg cgtcggaggg ccggccgtcg ccggagggca ccgttgaaga
     1981 cgcggtggcc agcgtccgct acgcgtccgc gcggctggcg cccggggccc ggggcgtggt
     2041 cctcttcggc cagtccctgg gggtggcggt ggccgtgggc gccgcggttc gggaaccgtc
     2101 ggtcaaggcc ttggtcctgg aggggggatt taattcctac cgggtgatca cccggacggt
     2161 ccttcgtcgc agctggattt tatggcccgt ttcggcgatc ctgcccccga tcctcgtccg
     2221 taaaacctgg gacccggaca aaatcgtggc cgaaatctcc ccccgcccgc tcctgtttat
     2281 ccacggcacc gcggaccggg tggtgccggc ggttttgacg gaacgcctct tcgcccgggc
     2341 ccgggacccc aaggaactct ggttgattcc cggggcgggg catttgcaat gccaccgcgt
     2401 ggccggcgcg gcttacgagg aaaaaatcat cggcttttac cgccgggccc tgggacttac
     2461 agaaaataat tgagggaggt cgcggcttcc cgataaattc ccgtccggga aatttccgtc
     2521 cgccaaagcc agcggggcga cagcgaccac cccgccgtca ggttccaacg ggtcacggtg
     2581 tccacctgcc cccaaccgta gcggatcacg cccccctccg ccgatatttt caacggcgaa
     2641 taagac
//
LOCUS       DAOQLS010000251         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23263_length_2645_cov_4.757529, whole genome shotgun sequence.
ACCESSION   DAOQLS010000251 DAOQLS010000000
VERSION     DAOQLS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23263_length_2645_cov_4.757529"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..372)
                     /locus_tag="PK362_05595"
     CDS             complement(22..372)
                     /locus_tag="PK362_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74341.1"
                     /translation="MVYSVPSPERSEASLPPERIVARLEDLLGREAVLLERGLWTKLG
                     PLGRQIAGFWNRLDRHPAFLQSIEGKAALTRIRQRVEANVGRLKTLSGDLRAGVDRER
                     RLGRARRAYGGTRI"
     gene            complement(422..1345)
                     /locus_tag="PK362_05600"
     CDS             complement(422..1345)
                     /locus_tag="PK362_05600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNC74342.1"
                     /translation="MGQSEGMKRISAFIQKISSVTSSVLIRGETGTGKEIAAHGIHNR
                     SPRRDGPFIAVNCSALPETMLESELFGHEKGAFTDATGLKRGLLEVSGGGTLFLDEIG
                     DMPLALQAKLLRVVETKRFRRLGSTVEHAVDVRFLAATHRDLEEMVRKGQFREDLFYR
                     LSVFTLVLPPLRERREDIAPLADFFLKGLTAGRAPKTLSPDALEHLVAYPWPGNVREL
                     HNVMEHVVVFSDAEVIAPADLPPHVMTGRRMACLEPLAAVERRHVERVFAAFGGDVRQ
                     TAKSLGISESDLAARMARYRDAGPLPPSPLH"
     gene            complement(1345..1800)
                     /locus_tag="PK362_05605"
     CDS             complement(1345..1800)
                     /locus_tag="PK362_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74343.1"
                     /translation="MTATKTPAETALKILIVDDDRSAAYALDRALTENGHEVAYAFSA
                     EQGLKSLKESPVDVVLLDLVMPGMDGTEMLRQVKSRHPAIDVVTMSGAGGMRAALGTL
                     QLDAFDFLPKPIQWQALEDVIGRLRQKRQKSKAGGGRIARCPPSSPSPI"
     gene            1948..>2645
                     /locus_tag="PK362_05610"
     CDS             1948..>2645
                     /locus_tag="PK362_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNC74344.1"
                     /translation="MQPDLASIIGVLAGFGLILLGQILEGGHVSSILQPTAALIVFGG
                     TLGATLTCFPLNVAIGSLKRFISVFFGQHHNAATLAKELLEYAALSRKEGLLALQKFQ
                     PNIKEEFLSKGLQLVIDAVPEKALREMMEREMTEAEQHDEHPVKFFEAAGGFAPTVGI
                     IGAVLGLIHVMENLADPSTLGAGIAVAFVATIYGVASANLLFLPASAKLKHHKEANNR
                     RRELILDALVAIQQG"
BASE COUNT          481 a          813 c          849 g          502 t
ORIGIN      
        1 cggtccatgg acaggagggg gttagatgcg cgtcccgccg tacgcccggc gggcccgacc
       61 cagacgacgc tcccgatcga ccccggcccg gaggtcgccg gaaagcgttt ttagccgccc
      121 gacgttcgct tcgacgcgtt gccgtatccg cgttaacgcg gctttccctt cgatggattg
      181 gagaaaggcg ggatgccggt cgaggcggtt ccaaaatcca gcgatttgac gccccagggg
      241 accgagcttg gtccaaagac cccgctccag aaggacggcc tcgcggccca gcaaatcctc
      301 caggcgggcg acgatgcgtt cgggcgggag agaggcctcg gaccgttcgg gcgaagggac
      361 ggaataaacc atggcctcgt tcccaacagc agggatcgtg ccaggcgtgc ggaaggtcgg
      421 tttagtggag gggcgagggc ggcaggggac cggcgtcccg ataccgggcc atccgggcgg
      481 ccaggtccga ttcggaaatg ccgagggatt tcgccgtttg tcgaacgtcc ccgccaaagg
      541 cggcaaaaac ccgttccacg tggcggcgtt ccacggccgc caggggttcc aggcaggcca
      601 tgcgacggcc ggtcattacg tggggcggca aatcggccgg ggcgatgact tcggcgtcgg
      661 aaaacaccac cacgtgttcc atcacgttgt gcaattcgcg cacgttgccg ggccaggggt
      721 aggcgaccaa atgctccagg gcgtccgggg agagggtttt gggggcgcga ccggcggtca
      781 atcccttgag gaaaaaatcc gccaggggcg cgatgtcctc ccggcgttcc cgtagaggcg
      841 gcaacaccag ggtgaaaacg cttaaccggt aaaacaaatc ttcccggaac tggccctttc
      901 gaaccatctc ttccaggtcc cggtgggtgg cggccaaaaa acggacgtcg accgcgtgct
      961 ccacggtgct tcccaaccgc cgaaatcgtt tggtttcgac gacccgaagg agtttggcct
     1021 gaagggccag gggcatgtcc ccgatttcat cgaggaacag cgttcccccg ccggaaactt
     1081 ccaggagccc ccgtttcaat ccggtggcat cggtgaaggc gcccttttcg tggccgaaca
     1141 gttcgctttc cagcatcgtt tcgggcaggg cgctgcaatt gaccgcgata aaggggccgt
     1201 cgcgccgagg gcttcggttg tggatgccgt gggccgcgat ttcttttccg gtcccggttt
     1261 ccccccggat caggacgctg gaggtgaccg aggaaatttt ctgaatgaag gccgaaatcc
     1321 gcttcatccc ttcgctttgt cccatcaaat cggggacggt gaagagggcg gacagcgggc
     1381 gatccttccc ccccccgcct tggatttttg gcgtttttga cggaggcggc cgatcacgtc
     1441 ctccagcgcc tgccattgga tcggtttggg gagaaaatcg aaagcgtcca gctggagggt
     1501 gccgagcgcc gcccgcatgc cccccgctcc ggacatggtg accacgtcga tggccgggtg
     1561 gcggcttttg acctggcgga gcatttcggt gccgtccatg cccggcataa ccaggtccaa
     1621 aaggacgacg tccacggggg attccttcag gctcttgagt ccctgctcgg cggaaaaggc
     1681 gtaggcgact tcgtggccgt tttccgtgag ggcccggtcc agggcgtacg cggcggaccg
     1741 gtcgtcgtcc acaatgagaa tcttaagggc ggtctcggcg ggggtcttgg tggcggtcat
     1801 ggtgttcctc gttgttcggg cggcccgatc cctcgcgcgg gccgcttggt gcgaacctta
     1861 tataccccgc cctcgctcga ctgtcaaggc gtgaactcaa taaaggcgtc cttcggccga
     1921 tagtgagggg caaggacagg aggcggcatg caaccggatt tggcgtccat catcggcgtg
     1981 cttgcggggt ttggcctgat tttattgggc caaattctcg aaggcggcca tgtttcgtcc
     2041 attttgcagc ccacggccgc cctcatcgtg ttcggcggca ccttgggcgc cacactgacc
     2101 tgttttcccc taaacgtggc catcggctcc ttaaagcgtt ttatctcggt tttcttcggt
     2161 cagcaccaca acgcggccac actggccaag gaactcttgg aatacgcggc cctcagccgc
     2221 aaggaaggcc tgctggctct ccaaaaattt cagccgaaca tcaaggaaga gttcctttcc
     2281 aagggcctcc agctggtcat cgacgccgtc cccgaaaagg cccttcggga aatgatggag
     2341 cgggaaatga ccgaagccga acagcacgat gagcaccccg taaaattttt cgaagccgcc
     2401 gggggtttcg cgccgaccgt cggcatcatc ggcgccgtgt tggggctcat tcacgtcatg
     2461 gaaaacctcg ccgacccctc gaccttgggg gccggcatcg cggtcgcctt cgtcgcaacg
     2521 atttacgggg tggcctccgc caacctcctg tttttgcccg cttcggccaa actcaaacac
     2581 cacaaggaag cgaacaaccg gcgccgtgaa ctgattttgg acgccctggt ggccatccag
     2641 caggg
//
LOCUS       DAOQLS010000252         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23421_length_2635_cov_3.762791, whole genome shotgun sequence.
ACCESSION   DAOQLS010000252 DAOQLS010000000
VERSION     DAOQLS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23421_length_2635_cov_3.762791"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK362_05615"
     CDS             complement(<1..282)
                     /locus_tag="PK362_05615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463630.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC74345.1"
                     /translation="MSDTVKYLLDETRLPKFWYNLMADLPKPPPAVLHPGTQKPIGPS
                     DLEPLFPMSLIQQEVSTEREIEIPEPVRAIYRQWRPSPLFRARRLEKALG"
     gene            411..863
                     /locus_tag="PK362_05620"
     CDS             411..863
                     /locus_tag="PK362_05620"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC74346.1"
                     /translation="MGLKRFTSKAFTLIELMLVVAIIGLLSAIALPKFANLVIKAKEA
                     ALKGKLGALRSAISIYYADNEGRFPILAQTSNWSNLTTGARYLDAPLRASIPTYPAHV
                     EDIGFWSNPGTDWLFNGDNKAWLYISGTGKMVVNCLHTDSGGITWSLW"
     gene            860..1285
                     /locus_tag="PK362_05625"
     CDS             860..1285
                     /locus_tag="PK362_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC74347.1"
                     /translation="MKPSDRGGFTLIELMIVVAIIGLLAAIALPKFANLVTKAREATI
                     KGKLGSLRSALSLYYADTAGLSPFAAFYVGQCLAPRYIESVPSIQIPGYPSHVSGNNF
                     TGTIDDAAMTGGFPWCYISQRLVISCTHTDSKGTVWSEW"
     gene            complement(1336..2433)
                     /locus_tag="PK362_05630"
     CDS             complement(1336..2433)
                     /locus_tag="PK362_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNC74348.1"
                     /translation="MNPIDPHSHNEIRPAEEIRTESFNAWDPAITQQIDALVAKAAEA
                     ARSDGDPDTTDLAREIIVTALKTQQAHLSRGDVKILSRAIRELRYGFRVFRPYRDRRK
                     VTIFGSARTRPADIDYKQARAFARKMVKKGFMVITGAGPGIMQAGNHGAGAENSFGIN
                     IKLPFEQSANEFVDKGERFIDCRFFFTRKLMFIKETSAVALFPGGFGTHDEGMEVLTL
                     VQTGKCDPMPIVFIETPGGSYWSDWEKYVRKHLLGKKKISEEDLSLFKVTDSVSEAAE
                     EIATFYRNYHSLRYVKDRMVIRLQKAPAEMPLKMLNRDFKDIVLPGASMTISKALPEE
                     SEWPDLPRLVFPFNRINYGRLRQLINALNAL"
BASE COUNT          487 a          751 c          823 g          574 t
ORIGIN      
        1 cccgagggcc ttttccaacc gccgggcccg gaaaagcggc gagggacgcc attggcggta
       61 gatggcccga accggttcgg gaatttcaat ctcccgctca gtcgagacct cctgttggat
      121 cagcgacatc gggaacaggg gctccaaatc cgacgggccg atgggctttt gggttcccgg
      181 gtggagcacc gcggggggcg gcttcggcaa gtccgccatc aggttgtacc aaaacttggg
      241 aagacgggtt tcgtccagca ggtatttcac ggtgtcgctc ataatcccct ccgtcgatgg
      301 tgatcccgtt gccgccctat tctaccattc ggggaaacgg gttcgcgcgg cggttcctcc
      361 gggggttgtt ttttcgcctt tcggggatac ccttgagggg ggaggtatga atgggcctaa
      421 aacgcttcac gtccaaggct ttcacgctga tcgaactcat gctcgtggtg gccatcatcg
      481 gcctcctgtc ggccatcgcc ctgcccaaat tcgccaacct cgtcatcaag gcgaaggaag
      541 cggccctgaa aggaaagttg ggagcccttc gaagcgcgat atcgatttac tacgccgaca
      601 acgaaggtcg ttttcccata cttgcccaga ccagcaattg gagcaattta accaccgggg
      661 cccgatattt ggatgcgccc cttcgagcgt ccatcccgac ttatcccgcc catgtggagg
      721 acattggatt ttggagcaac cccggcacgg actggctttt taacggcgat aacaaggcct
      781 ggctatacat ttccggcacg gggaaaatgg tcgtcaactg cctccacacg gactcgggcg
      841 gaatcacctg gagtctctgg tgaaaccctc cgaccgcggg ggtttcacac tgatcgaact
      901 catgattgtg gtggccatca tcggcctcct ggcggccatc gccttgccca aattcgccaa
      961 tttggtgaca aaggcgagag aggcgacgat taaaggaaaa ctgggaagcc ttcggagcgc
     1021 tttgagcttg tattacgcgg acaccgccgg gctgtcgccc ttcgcggcct tttacgtcgg
     1081 gcaatgcctc gccccccgct acatcgaaag cgttccatcg atacagatcc ccggctaccc
     1141 cagccacgtt tcggggaaca attttacggg gaccatcgac gacgccgcca tgaccggagg
     1201 gtttccctgg tgttatatct cgcaacgttt ggttatttcc tgcacccaca cggactccaa
     1261 aggcaccgtt tggagcgagt ggtagctgac ctggggaata ttcccgggcc tcaggcggaa
     1321 agacgggcaa agaccttata gcgcgtttaa ggcgttgatc agctggcgaa ggcggccgta
     1381 gttgatgcgg ttgaagggaa acacgaggcg gggcaaatcg ggccactcgg attcctccgg
     1441 cagggccttg gaaatggtca tggaggcgcc gggcaggaca atgtccttga aatcccggtt
     1501 gagcattttc agcggcattt cggccggggc cttttgcagg cggatcacca tgcggtcctt
     1561 cacataacgc agggaatgat agttgcggta gaaggtcgca atttcctccg ccgcttcgct
     1621 caccgaatcc gtgactttga acaggctcaa atcctcttcg gaaatctttt tcttccccag
     1681 cagatgtttc cgaacgtatt tttcccaatc cgaccaatag ctcccgcccg gcgtctcgat
     1741 gaagacgatg ggcatggggt cgcatttgcc ggtctggacg agggtcaaca cttccatgcc
     1801 ctcgtcgtgg gtgccgaaac cgccggggaa aagggccacc gcgctggttt ccttgatgaa
     1861 catcaatttc cgggtgaaga aaaatcggca gtcgatgaac cgctcgccct tgtccacgaa
     1921 ctcgttggcg gactgttcga agggcagttt gatgttgatg ccgaaactgt tttccgcccc
     1981 ggccccgtgg ttgccggcct gcatgatgcc gggcccggcc cccgtgatga ccatgaagcc
     2041 cttcttgacc atttttcggg cgaaggcccg ggcctgtttg tagtcgatgt cggcggggcg
     2101 cgtacgggcg gaaccgaaaa tggtgacctt gcgccggtcc cggtaggggc gaaacacccg
     2161 gaacccgtaa cgcagttccc ggatggcgcg gcttaaaatc ttgacgtcgc cccgggacag
     2221 gtgggcttgc tgggttttca gggcggtgac gatgatttcc cgggccaggt cggtggtgtc
     2281 ggggtcgccg tcgctccggg cggcctcggc cgccttggcg accagggcgt cgatctgctg
     2341 ggtgatggcc gggtcccagg cgttgaagga ctccgtgcgg atttcttccg cggggcgaat
     2401 ttcgttgtgc gagtgggggt caatgggatt catggtgtcg gctctttccg ttgcgcagtg
     2461 ggttcttgtg gtctcttttt cgtttcttaa ggatggatgg gaatcgcggt tcccgcaagg
     2521 ggcgggccgt caccggtaat gggaccagac ggcgtcggcc gtgacgggct ttcgaggggt
     2581 ccattttcgg aaccaatggc gggcgtagcc cccggccagg tacgcccagg cgtgg
//
LOCUS       DAOQLS010000253         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23558_length_2626_cov_4.720342, whole genome shotgun sequence.
ACCESSION   DAOQLS010000253 DAOQLS010000000
VERSION     DAOQLS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23558_length_2626_cov_4.720342"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="tig"
                     /locus_tag="PK362_05635"
     CDS             complement(<1..1038)
                     /gene="tig"
                     /locus_tag="PK362_05635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNC74349.1"
                     /translation="MFGWKKKGKEGAGTESESEAPRADVKVKVKDRKNCAVLLAVEVP
                     ADQVRKATEDSFRRVQSRAKLPGFRPGKAPMEMVKKNFQDAAWEDALDRLLRESVYSA
                     VTQENVVPITTPVVDKIDGEPTKALRFEVKVECAPTVEPKDYKGLPLQKKSRTVTDAD
                     TDKRLNDLRESNAKLVLSKDASVDKKHFVVADYDSFLDGQPVANGSAKNQLIEMAAPQ
                     NVEGFTEGLLGAKDGETREVPVRFPDVHPQKELAGKTVLFKVTVTAIKEKEMPALDDE
                     FAKDLGVESLAALKERLRKDLETGAERALRQDLEKQVIDGLLQRNVFDVPPSQTEERA
                     RHLTAQVKDYLA"
     gene            complement(1126..1207)
                     /locus_tag="PK362_05640"
     tRNA            complement(1126..1207)
                     /locus_tag="PK362_05640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1171..1173),aa:Leu,seq:tag)
     gene            complement(1274..1345)
                     /locus_tag="PK362_05645"
     tRNA            complement(1274..1345)
                     /locus_tag="PK362_05645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1311..1313),aa:Lys,seq:ctt)
     gene            complement(1429..1502)
                     /locus_tag="PK362_05650"
     tRNA            complement(1429..1502)
                     /locus_tag="PK362_05650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1466..1468),aa:Arg,seq:tcg)
     gene            complement(1690..>2626)
                     /locus_tag="PK362_05655"
     CDS             complement(1690..>2626)
                     /locus_tag="PK362_05655"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNC74350.1"
                     /translation="FGNGREVYRRFVQPVVTDLPRMAAHQALRTANGGVDGGRVYAYD
                     FESKDVRADRAGGRTLTSGRLSVRSRVTGERMDAHYAVLHLGGHDFRCLLRSGDDAET
                     FQDLRRSLAEQFARNDGETFFQAIRKEFGPHVYTLQDLLLEERRRMLSTVIERILGEF
                     DQTHRRLVTENRTLIDYLQRADHPIPHAFRLALESVLGRDLSAALARFNGEESTAEAL
                     RRVRREAATYHVHLHWSSVTKEIECHFLGRVRQLVRSGNALDADKALFLLNLAEELDL
                     TPTLWEAENLFFTFWKNTNDRRPWEALARRLRFAD"
BASE COUNT          464 a          797 c          823 g          542 t
ORIGIN      
        1 ggccaggtaa tccttgacct gggccgtgag gtgacgggcc cgttcctcgg tttgggaggg
       61 cgggacgtcg aacacgttcc gttgaagcaa cccgtcgatg acttgttttt ccaaatcctg
      121 gcgaagggcc cgttccgcgc cggtctccag gtctttgcgc aggcgttcct tgagggcggc
      181 caggctttca acgccgaggt ccttggcgaa ttcgtcgtcc aaggccggca tttccttttc
      241 tttgatcgcg gtcacggtga ccttaaagag aaccgttttt cccgccagtt ccttttgggg
      301 gtgcacgtcc gggaaccgga cggggacttc ccgggtttct ccgtctttgg cgcccaggag
      361 cccttccgtg aacccttcca cgttttgggg agcggccatc tcgatcagtt ggtttttggc
      421 ggagccgttg gccacgggtt gaccgtccag gaaactgtcg taatcggcca ccacgaaatg
      481 ctttttgtcc acggaggcgt ccttggagag caccagtttg gcgttggatt cccgcaggtc
      541 gttcaatcgt ttgtcggtgt ccgcgtcggt caccgttcgc gatttctttt gaaggggcag
      601 gcctttgtaa tccttgggtt cgacggtcgg ggcgcattcc accttgactt cgaaccgcag
      661 ggctttggtc ggttcgccgt cgatcttgtc caccacgggc gtcgtgatgg gcacgacgtt
      721 ttcctgcgtc acggcgctgt agaccgattc gcgcaggagc cggtcgagag cgtcttccca
      781 ggcggcgtcc tgaaaattct ttttcaccat ctccatgggg gccttgcccg ggcgaaatcc
      841 cggcaatttg gcccgggatt ggacccgtcg gaaggaatct tccgtggctt tgcggacttg
      901 atccgccggc acttcgaccg cgagcagtac ggcgcaattt ttgcggtctt tgactttgac
      961 tttgacgtcc gcccgggggg cctccgactc cgattccgtc cccgcgccct ctttgccctt
     1021 ttttttccaa ccgaacataa aggatctccg tgtcttttta tttgcccatc cgctttgcgc
     1081 gcgccatcgg cggccgcggg ggtgtcgtgt cccgtcgttt aaatctgcga ggagagggac
     1141 ttgaacccac acgcctttcg gcatacggtc ctaagccgta cgcgtctgcc aattccgcca
     1201 tcctcgcgaa gtcccgccgg gccgtgggcc gcggcggtcc tgaaatcgcc aaagggatga
     1261 ggctgaaaaa aaatgggcca ggagagactc gaactctcag ccttgcgctt aagaggcgct
     1321 tgctccaccg ttgagctact ggcccatggc cgccgggcgg gttcctcccc cgatccgggg
     1381 gtacgccccg cggaagaaag acgcccggaa cggcgttgat gaaaatctcg cgcccggagg
     1441 gacttgaacc cccagccctc tgctccgaag gcagatgctc tatccaattg agctacgggc
     1501 gcaaatccaa ccgggacgtt ttccgtgaaa tccggccttc gacgtccgag ccggccggcc
     1561 aagcttcggg ccggcggttc ccccgtccga ggcgcgacgg atagaaaaac cccccggggg
     1621 tgcgacaacg cggggggtcg ggaccgagaa cgaagtataa caaaaacggc ggaggggcga
     1681 aacgaggaat taatcggcaa atcgaagacg gcgggccaag gcctcccagg ggcgtcgatc
     1741 gttcgtgttt ttccaaaagg taaagaaaag attttccgct tcccacaaag tgggggtgag
     1801 gtccaattcc tcggccaaat tgagcaggaa aagcgctttg tcggcgtcga gcgcgttccc
     1861 cgagcggacc agttggcgaa cgcggccgag gaaatggcat tcgatttcct tggtgacgct
     1921 cgaccaatgg aggtggacgt ggtaggtcgc cgcttcgcgc cgaacgcggc gaagcgcctc
     1981 cgccgtggac tcctcaccgt tgaaccgcgc cagcgcggcg gaaaggtccc gccccagtac
     2041 ggattcgagg gcgagccgaa aggcgtgcgg aatggggtgg tcggcccgtt gaaggtaatc
     2101 gatgagggtt cgattttcgg tcaccaggcg gcggtgggtt tgatcgaatt cccccaggat
     2161 gcgttcaatg accgtggaca acattcgacg ccgttcctcc agcaacaaat cctgaagagt
     2221 gtatacgtgg gggccgaatt cctttcgaat cgcctggaag aaggtctcgc cgtcgttccg
     2281 ggcgaattgc tccgcgagcg atcgacgaag gtcctgaaag gtttcggcgt catcgccgga
     2341 gcggagcagg catcgaaaat cgtgtccgcc gaggtgcaac acggcgtagt gggcgtccat
     2401 ccgctccccc gtcacgcgcg aacggacgga cagccgtccc gacgtcagag tccgcccccc
     2461 ggcgcgatcc gcgcgaacgt ctttcgattc gaaatcatac gcgtagacgc gccccccgtc
     2521 cacgccgccg ttcgccgtcc gaagcgcctg gtgggccgcc atgcggggca agtccgtcac
     2581 gaccggctgg acgaaccgtc gatacacttc gcggccgttg ccgaac
//
LOCUS       DAOQLS010000254         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23649_length_2620_cov_4.819493, whole genome shotgun sequence.
ACCESSION   DAOQLS010000254 DAOQLS010000000
VERSION     DAOQLS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23649_length_2620_cov_4.819493"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..617
                     /locus_tag="PK362_05660"
     CDS             117..617
                     /locus_tag="PK362_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74351.1"
                     /translation="MAVVLCFGPVLLGAAAPDVSDVRAVFKDVETLRAAGKLKKETAP
                     MSEFEEASLYTDDGGRSRLYVESLGGQDSVVTVSAYYDASENLRFVFCQAGAVNDSKM
                     ERRYYFGLDGKKIDQKEKLTAGEGYPWDWESVEKTTVFGRARSPRESFKRFASGADGK
                     KETVSP"
     gene            614..2140
                     /gene="guaA"
                     /locus_tag="PK362_05665"
     CDS             614..2140
                     /gene="guaA"
                     /locus_tag="PK362_05665"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNC74352.1"
                     /translation="MIIILDFGSQYTQLIARRVREANVFCEIHPYNYSAKKIAASKPS
                     GLILSGGPSSVYDKKAPKPDADIFGLDIPILGICYGLQVMVTHFGGQVAKAPRREYGP
                     ADIQIVHNNGLFDGLSETQPVWMSHGDHAEGLPAGFQTMAKTSNAPYAAVFNPARKFY
                     AVQFHPEVHHTPRGKDILANFLFKVCGEKPDWTMTSFAEEQTRLVREIVGKDKVILGL
                     SGGVDSSVAAALLHKALGKQLHCIFVNNGLLRAGEEERVKKVFGKAQGYVTTIVDARK
                     QFLKKLKGVTDPERKRKIIGHEFIAVFEKEAKKHKDAKFLAQGTLYPDVIESVSVKGP
                     SAVIKTHHNVGGLPERMKMKLVEPLRFLFKDEVRALGKELGLPDEILFRQPFPGPGLA
                     VRVLGDLTEEKLRMAREADWIIEEEVRAAGLYHKLWQAFAVFLPVNSVGVMGDERTYQ
                     NAIAVRAVNSVDAMTADWARLPYDVLAKISSRITNEVRGINRVVLDISSKPPATIEWE
                     "
     gene            2131..2385
                     /locus_tag="PK362_05670"
     CDS             2131..2385
                     /locus_tag="PK362_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74353.1"
                     /translation="MGVNNLVIGGACLALGGGFYRLAARATTTGKLLRRDSTVAPRYW
                     DREKDRAEFNARVRILRGMALLVGALGLGLIYAGVFKSGR"
     gene            2396..>2620
                     /locus_tag="PK362_05675"
     CDS             2396..>2620
                     /locus_tag="PK362_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNC74354.1"
                     /translation="MTTTTIIDKTNLPFKVWTRGKVRDVYDLGDKLLIVATDRISAYD
                     FILPTAVPEKGKILCQTSNFWFNRLAAICPN"
BASE COUNT          532 a          819 c          791 g          478 t
ORIGIN      
        1 gtcgacctgg ccgtggacgg ccgccaggaa ggcgctctgc cgctcttttt gaagcccctt
       61 tcgaattccg ttcgggaacg gccatgatcg acgttcctcg tcgtgcggca cgggcgttgg
      121 ccgtcgtcct gtgcttcggc ccggtgttgc tgggagcggc cgccccggac gtcagcgacg
      181 tccgcgcggt gttcaaagac gtggagacgc ttcgcgccgc cggaaaattg aagaaagaaa
      241 cggcgcccat gagcgaattc gaggaggcgt ccctttacac cgacgacggg ggccggtcgc
      301 ggctttacgt cgaatccctc ggcggacagg actcggtggt gacggtcagc gcctactacg
      361 acgcgtcgga gaatttgcgg ttcgtttttt gccaggccgg ggccgtcaac gactccaaaa
      421 tggagcgacg gtattacttc gggttggatg gaaagaagat cgaccaaaaa gagaagctga
      481 ccgcggggga agggtacccc tgggattggg aatccgtcga gaaaacgacg gtttttggcc
      541 gggcgcggtc cccgcgcgaa tcttttaagc ggttcgcgtc gggcgcggat ggaaaaaagg
      601 agacggtgtc cccatgatca tcattctcga tttcggttcc cagtacacgc agctcatcgc
      661 ccgccgggtg cgggaagcca acgtgttctg cgaaatccac ccctacaact actccgccaa
      721 aaaaatcgcc gcgtccaagc ccagcggcct catcctctcc ggcgggccct ccagcgtcta
      781 cgacaaaaag gcccccaagc ccgacgcgga catcttcggg ttggacatcc ccattctggg
      841 catctgctac gggcttcagg tcatggtcac ccatttcggg ggccaggtgg ccaaggcgcc
      901 ccgtcgggaa tacggcccgg ccgacatcca gatcgtccac aacaacggcc tcttcgacgg
      961 tttgtccgag acccagcccg tgtggatgag ccacggcgac cacgccgagg gcttgccggc
     1021 cggatttcag accatggcga aaacctccaa cgcgccctac gccgccgtgt tcaacccggc
     1081 ccgcaaattc tacgccgtgc agttccaccc cgaagtccac cacacccccc ggggcaagga
     1141 catcctggcc aacttccttt tcaaagtctg cggagaaaag cccgactgga ccatgacctc
     1201 cttcgccgag gagcaaaccc gcctggttcg ggaaatagtc ggcaaggaca aggtgatcct
     1261 cggcctctcc ggcggggtgg attcctccgt ggccgcggcc ctcctgcaca aagccctggg
     1321 aaaacagctc cactgcattt tcgtcaacaa cggcctgctc cgcgccgggg aggaggaacg
     1381 ggtcaagaaa gtcttcggca aggcccaggg gtacgtcacc accatcgtcg acgcccgaaa
     1441 acagtttttg aagaaactca aaggcgtcac cgaccccgaa cgtaaacgga aaattatcgg
     1501 ccacgaattc atcgcggtgt ttgagaagga agccaaaaaa cacaaggacg ccaagttcct
     1561 cgcccagggc acgctctacc cggacgtgat cgaaagcgtc tcggtcaaag gcccctcggc
     1621 ggtcattaag acccaccaca acgtgggcgg cctgcctgaa cggatgaaaa tgaaattggt
     1681 ggagcccttg cggttccttt ttaaagacga agtccgggcc ctcggcaagg aactgggcct
     1741 gcccgacgaa atccttttcc gccagccctt ccccgggccc ggcctggcgg tgcgcgtcct
     1801 gggcgacctc acggaggaga aactgcgcat ggcccgggag gccgattgga tcatcgaaga
     1861 ggaggtccgg gccgccggtt tgtaccacaa actgtggcag gccttcgccg tgttcctgcc
     1921 ggtcaacagt gtgggcgtca tgggggacga gcgaacctat caaaacgcca tcgccgtccg
     1981 cgccgtcaac tcggtggacg ccatgacggc ggactgggcc cgcttgccct acgacgtgct
     2041 cgctaaaatt tcttcccgca tcaccaacga agtccggggc atcaaccggg tcgtgctcga
     2101 catctcctcc aagccgcccg ccaccattga gtgggagtaa acaacctcgt catcggcggg
     2161 gcgtgtctgg cgctgggggg cggtttttat cgtttggccg cccgggcgac gaccacggga
     2221 aagttgctcc gtcgggattc gaccgtggcg ccgcggtatt gggatcggga gaaagatcgg
     2281 gcggaattca acgcccgcgt tcgaattctt cgcggaatgg ccctgctggt cggggccctg
     2341 gggttggggt tgatctacgc gggcgttttt aaatcgggcc ggtgaacttg taaccatgac
     2401 gacgaccacc atcatcgaca aaacgaacct tcctttcaaa gtttggaccc gcggcaaggt
     2461 ccgggacgtc tacgatttgg gcgacaagct cctgatcgtg gccacggatc ggatttcggc
     2521 ctacgatttc atcctgccca cggcggtccc ggaaaaaggg aagatcctct gtcaaacgtc
     2581 gaatttttgg ttcaaccggc tcgcggccat ttgccccaac
//
LOCUS       DAOQLS010000255         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23747_length_2613_cov_4.055121, whole genome shotgun sequence.
ACCESSION   DAOQLS010000255 DAOQLS010000000
VERSION     DAOQLS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23747_length_2613_cov_4.055121"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..494)
                     /locus_tag="PK362_05680"
     CDS             complement(120..494)
                     /locus_tag="PK362_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74355.1"
                     /translation="MAYLLIVDDENTTSDLLIAVLQKDGHTLHTAKNGAEALATLGER
                     TPDLILMDVSMPEMDGYTMVSHLSADTATRDIPVIVMTGKNALEDTFRQFTNVADFFA
                     KPFDVKVLRQRVLQILAQHGRR"
     gene            complement(548..988)
                     /locus_tag="PK362_05685"
     CDS             complement(548..988)
                     /locus_tag="PK362_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74356.1"
                     /translation="MENPQPNYKAMRRNYLVNKRLQIKYAIIISAVLALMVVLMQAHT
                     FLVIRTIEPENATPGMIETMHSLQLWMLSSGLIYMVVIPILSIFISHKIAGPLYRLEK
                     SIREALDTEGPIRPIKLRDGDELHSLAELINKLFDRLPQNKKSN"
     gene            complement(1109..2164)
                     /gene="prfB"
                     /locus_tag="PK362_05690"
                     /pseudo
     CDS             complement(1109..2164)
                     /gene="prfB"
                     /locus_tag="PK362_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(2308..>2613)
                     /locus_tag="PK362_05695"
     CDS             complement(2308..>2613)
                     /locus_tag="PK362_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74357.1"
                     /translation="VVDALVRVVNPVSVVGLVLALVALILLTQIPVLRPLALVLGVLV
                     LGVLAAFLLLPVLGKIAQELAQRLAGFRGEVEAALRDVEARCPPACRGDVSPPECKL"
BASE COUNT          478 a          763 c          832 g          540 t
ORIGIN      
        1 actccatgcg ggtcgccacg tccgtcagcg ctccctgcag cgcggccacc ttggcccggc
       61 gttgggccat caattcgtcg agcgagctcg tgcggggggt tgtttcggcc acgaaaaggt
      121 taacggcgcc cgtgctgggc gaggatttgc aggacgcgct gccggaggac tttcacgtcg
      181 aaaggtttgg cgaagaagtc cgccacgttg gtgaattgcc ggaaggtgtc ttccagggcg
      241 tttttgcccg tcatgacgat cacggggatg tcccgggtgg cggtgtcggc gctcaagtgg
      301 gacaccatcg tgtaaccgtc catctcgggc atggaaacgt ccatgaggat caggtcgggg
      361 gtccgctcgc ccagggtggc cagagcctcg gcgccgtttt tggcggtgtg gagggtgtgc
      421 ccgtcctttt gaaggacggc gatcaacagg tccgaagtgg tgttctcgtc atcgacaatc
      481 aacaggtagg ccatggggct agtgtatcac gccccggcgg gacctgtcaa aagagttcga
      541 agaacggtca gttggacttt ttgttctgag gcagacggtc gaaaagcttg ttgatcaatt
      601 cggccaggct gtgaagctcg tccccgtccc ggagtttgat gggccggatg ggcccctcgg
      661 tgtccagggc ttcccgaatg gatttctcca accggtaaag gggcccggcg attttgtggg
      721 aaatgaagat ggacaggatc ggaatgacca ccatgtagat caaaccgctg gacagcatcc
      781 acaactgaag ggaatgcatg gtttcgatca tgcccggggt ggcgttttcg ggttcaatgg
      841 tccgaataac caggaaggtg tgggcctgca tgaggacgac catcaaggcc aagaccgcgc
      901 tgatgatgat ggcgtacttg atttggaggc gcttgttgac caaataattg cgccgcatgg
      961 ccttgtagtt cggctgtggg ttctccatcg aatcctcctc aatgtgggca ccgacaaaag
     1021 ccccgccctc tcccccggag gggaagacgg cgtctccgca attatagggc gcgttggccc
     1081 cctttgcaag gggacgccgc cccgccgctc aatccgatcc ggaccccttc cgcgggggcg
     1141 ccccggaaag cttccattcc aaataagcgt ggataaaggg attcaaatcg ccgtccatga
     1201 cggattggat ttgactggtt tcgtaaccgc ttcggagatc tttgaccatt tgatagggca
     1261 tgaacacgta actgcgtatc tgattgcccc aggcgatgtc gcccttgtcg tcgtggtgtt
     1321 tttcggtcgc ggcccgctgt ttttccatct ccaaatcgta gagccgggcc ttgagaagtt
     1381 tcaagcacat ggcgcggttt ttgtgctggc tgcgctcgat ctggcaggcc accacgacgc
     1441 ccgaggggac gtgggtgatg cgcacggcgg attcggtttt attgacgtgt tggccgcccg
     1501 ccccgcccga ccggaaggtg tcgactttca ggtccgcctc tttgatgtcg atttccacgt
     1561 cgtcgtcaat ttcggggatc acgtcgcagg cggcaaaaga ggtgtggcgg cgggcgttgg
     1621 cgtcgaaggg cgacacgcgc acgaggcggt gcacgccggc ctcggatttg aggttcccgt
     1681 aggcgaactc cccctccacc aggaactccg cccgtttgta gccggccccg tcgccgggca
     1741 tgaggctcgt ggtggtcact ttgaaaccat tggcttccgc ccaccgggtg aacatgcgga
     1801 aaagcatttc cgcccaatcg caggattccg ttccgccggc cccggcgtgg acggacacga
     1861 tggcgttttt agcgtccatg gggcccgaca acttggcctg gagagtgagg ctttcgattt
     1921 tttttccgac ggcgtccaat ccctgggaca attccgccca ttcggcctcg ccgcccccct
     1981 cctcgcacaa ttcgcggagg gcgtccaaat cgccgacctg gcgttccaac gcttcgtatt
     2041 tgccgatggc gtttttgagg gcggcggttt ctttaaggat tttctgggcg gcctggttgt
     2101 cgttccaaaa atcgggccgg gcggtctggg cttcgagggc ggtcagtttt tcgcgtttgt
     2161 tggggacgtc aaagaaacct ccccagccgg gccagcgatt cgcgggcgcg ggtcaagcct
     2221 tgggtcagtt gctcgacgct cggggtttcc acggggggac tttacttcag gcgcgggggg
     2281 ttgtcaatcg cgcggccggg gatcgcgtca aagtttgcac tccggtggcg agacgtcgcc
     2341 ccggcaggcg ggcgggcaac gggcctccac gtcccgaaga gcggcctcga cctccccacg
     2401 gaacccggcg agacgttggg caagttcctg ggcgattttc cccaacacgg gcagcaacag
     2461 aaacgccgcc agaacgccca gaaccagaac ccccaaaacc agagccaacg ggcgcaacac
     2521 cggaatctgc gtcaacagga tcagcgccac caacgccagg accaaaccaa cgacgctcac
     2581 cggattgacc actcgaacca acgcgtcaac gac
//
LOCUS       DAOQLS010000256         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23821_length_2608_cov_4.688210, whole genome shotgun sequence.
ACCESSION   DAOQLS010000256 DAOQLS010000000
VERSION     DAOQLS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23821_length_2608_cov_4.688210"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2608)
                     /locus_tag="PK362_05700"
     CDS             complement(<1..>2608)
                     /locus_tag="PK362_05700"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74358.1"
                     /translation="SAWVASLPPPAAAGVAVAAAMVAQAGLWVEVYLVSGLVLDGIYG
                     YAPARVPLYRHAAVGLQKGLAFSGLFLGILFAVKLGLSVAAVQEFLAGSPLLAGAVLG
                     ALVYPFAKAIVESFDGSMSFFRRAVYSYRNGTLYVRGAVAGAALAFGFTGGWAARSTG
                     ERLAFGLLAGALASAGISVLRDAVYAATRRGRVGVWKIYLVDGGLGAFIGAALAFYLD
                     AAQVPVLVEKFKLYTSSGLPSQNYTIYALLSKWGRVDLGSFAGGAKLFFNESLAGVLT
                     WAIAAWLFAVNRVFMTAYFQKDKQPIRHFFSRAGLVELGVNTIHVLRWGLWMAPIINT
                     GLRMMGHATWYNQDGAIRTLVAIVKNITLHPTDFQAWSLNLFVALLAYDAVRVLIWID
                     HMGLRVATLVNLSFLGMDKLDERIARFIGPSAAQRYIPEGVKRFTTWAPLLIPFYIPR
                     GADWDMAWETSQKMQRAAAEAGGLDAFLRGVSPLAWALWGAGALALFTGLIVLGRKRA
                     ERRSGVRAHAAELANREYKVVIKENGEFHSEILQKGYDVTRRSYDTLDPAGRTLFLVD
                     TEIPLGRPGRYWPLAGNFPRDRFDAPVVERFGDALRLMTSSHGVRATIDVRLPDRDTP
                     AELWTLTLENQSDQPRQLVAVPYVEWVLDRPDSDKGHTQYGRLFPEMEYVRESNAVLA
                     WQKKTKTMGYLAVQTAPRGFCTSRMGFIGRARSLWSPRLLETLKFLDPQDTAGYPTFD
                     PIAGLKIDVELGARESKTLRLILGSAKSREAALAVLQKHLRPETVAPASPARKGHRVP
                     LIGHGEIPPGTPQPYSDYSPDGNTLRVHTPFTPRPYDHALSNAVGHYVMVTNRGLHTT
                     SNGNSQQNPVTPD"
BASE COUNT          504 a          812 c          872 g          420 t
ORIGIN      
        1 atcgggggtc acggggtttt gctgggaatt gccgttggag gtcgtgtgga gcccccggtt
       61 cgtgaccatc acgtagtgcc ccaccgcgtt cgagagggcg tggtcgtagg ggcgcggggt
      121 aaagggcgtg tgcacccgca gggtgtttcc atcgggcgaa tagtcggagt acggctgggg
      181 cgttcccggt ggaatttcgc cgtggccgat cagcggcacc cggtggccct tccgggccgg
      241 gctggccggc gccacggttt cgggtcgaag gtgcttttgc agaacggcca gagccgcttc
      301 ccggcttttc gcgctcccca aaatcagacg gagagttttg gattcccggg cgcccaattc
      361 cacgtcgatt tttaagccgg cgatggggtc gaaggtgggg taccccgccg tgtcttgcgg
      421 atccaagaat ttaagggttt ccaacaaccg gggagaccag agacttcggg cgcgtccgat
      481 gaaacccatg cgggacgtac agaacccccg gggcgccgtt tgaaccgcca gataccccat
      541 ggtcttcgtc tttttctgcc aggccaggac ggcgttggat tcccggacgt attccatctc
      601 cgggaagagg cggccatact gggtgtgtcc tttgtcggaa tcgggtcgat ccagcaccca
      661 ctccacgtag ggaacggcca ccaattgacg gggctggtcg gactggttct ccagggtgag
      721 ggtccacagc tccgccgggg tgtcccggtc cggcagacgg acgtcgatcg tggcgcgcac
      781 gccgtgggag gaagtcatca aacggagggc gtcgccgaaa cgctcaacca cgggcgcgtc
      841 gaagcgatcc cgggggaaat tcccggccag gggccaatag cggcccgggc gcccgagcgg
      901 gatttcggtg tcgaccaaaa acagggttcg gccggcgggg tccagggtgt cgtaggaccg
      961 gcgggtcacg tcgtatccct tttgcagaat ttcactgtgg aattcgccgt tttccttgat
     1021 caccaccttg tactcccggt tcgccaattc cgcggcatgg gcccggaccc ccgaacgacg
     1081 ttccgcgcgc ttgcgaccaa ggacaatcag tcccgtgaag agggccaggg cgccggcgcc
     1141 ccagagggcc caggccaggg gcgaaacgcc ccggagaaaa gcgtccaaac cgccggcttc
     1201 ggcggcggcc cgctgcattt tttgggaggt ttcccaggcc atgtcccagt ccgctccccg
     1261 gggaatgtag aacgggatca ggagcggggc ccaggtcgtg aaccgtttga ccccttcggg
     1321 aatgtaacgc tgggcggcgg agggtccgat gaaacgggcg atgcgttcgt ccagtttgtc
     1381 catgccgagg aagctcaggt tgacgagggt cgccacccgc aggcccatgt ggtcgatcca
     1441 gatcaacacg cggaccgcgt cgtaggccag caacgccacg aacaagttca ggctccaggc
     1501 ttgaaaatcc gtggggtgga gcgtgatgtt cttgacgatg gccaccaggg ttcgaatcgc
     1561 gccgtcctgg ttgtaccagg tggcgtggcc catcattcgg aggcccgtgt tgatgatggg
     1621 cgccatccac agaccccagc gaagcacgtg aatcgtgttg acgcccaact ccacgaggcc
     1681 ggcccgggag aaaaagtgcc ggatcggctg cttgtccttt tgaaagtacg cggtcatgaa
     1741 cacccggttg accgcgaaca gccacgccgc gatggcccag gtcaggaccc cggccaggga
     1801 ttcgttgaag aacagtttgg ccccgccggc aaaggaaccc agatccaccc ggccccattt
     1861 gctgagaagc gcgtaaatcg tgtagttctg ggagggcaaa ccgctcgagg tgtaaagttt
     1921 gaatttttcc accaacacgg gcacctgggc ggcgtccaaa taaaaagcca aggccgcgcc
     1981 gatgaaggcc cccaatccgc cgtcgaccag ataaattttc caaacgccca cgcgaccgcg
     2041 gcgggtggcg gcgtaaaccg cgtcgcggag cacactgatc cccgccgaag cgagggcgcc
     2101 ggccagaagg ccgaaggcga ggcgttcgcc ggtggaacgg gcggcccacc cgccggtgaa
     2161 accgaaggcc agggccgcgc ccgccaccgc gccgcgaacg taaagggttc cgttccggta
     2221 gctgtagacc gctcggcgga aaaacgacat gctcccgtca aagctttcca cgatcgcctt
     2281 ggcgaaagga taaaccaggg ccccgaggac cgccccggcc aacagcggcg atcccgcgag
     2341 aaattcctgg accgcggcca cggacaatcc gagcttgacc gcgaacagaa tgccgaggaa
     2401 aagtccgctg aaggcgagac ctttttgcag gcccacggcg gcgtgccgat agaggggaac
     2461 ccgggcgggg gcgtatccgt aaatcccgtc caacaccagg ccggagacca gatacacctc
     2521 cacccacagg cccgcctggg cgaccatggc ggccgctacg gccacgccgg ccgcggccgg
     2581 cggcggcagg gaagcgaccc aagccgac
//
LOCUS       DAOQLS010000257         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23823_length_2608_cov_4.431257, whole genome shotgun sequence.
ACCESSION   DAOQLS010000257 DAOQLS010000000
VERSION     DAOQLS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23823_length_2608_cov_4.431257"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK362_05705"
     CDS             complement(<1..389)
                     /locus_tag="PK362_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNC74359.1"
                     /translation="MPDPFLADFVALNRRLGGDPLLSQGSGGNTSVKIDPDTLRVKAT
                     GVPLREVSENRGWVDMDLKALRAGVEKLWLLKGEEQQQRAYRALLSGAQKTPEPRVSI
                     ETNLHVLLADRWVAHVHSLGGQLLGLLP"
     gene            complement(382..1326)
                     /locus_tag="PK362_05710"
     CDS             complement(382..1326)
                     /locus_tag="PK362_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNC74360.1"
                     /translation="MKQTAPLRVGISACLLGQKVRYDGGHKRDAFAADLLGRFVEFVP
                     VCPEMEIGLGTPRESIHLKEVDGDVRLVAPRSGRDLTEDMHRYAGKRADALAREDLCG
                     FILKKGSPSCGLERVSVHRSGPPTKSGVGFFARALLARWPDLPVEDEGRLNDPRLREN
                     FLERVFATHRLRGLEKGLGALVRFHTAEKLLLMAHDPASYQTLGRLVANGKGRPWGEL
                     TADYRSAFLRALAVLPTRSRHVNALQHAAGYFRDKATEAERRELAGAIEDFRRELVPL
                     IVPVTLLRSAARVHGVDYLIGQTYLSPHPKELMLRNHA"
     gene            1391..2503
                     /locus_tag="PK362_05715"
     CDS             1391..2503
                     /locus_tag="PK362_05715"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC74361.1"
                     /translation="MKRATPFVLLTLVFFACAGGQPKVSTLVKQLRSSDSAVKIKAAN
                     ILAGLGSQAEPALPGLLTMFRDTRKPLHDAAAKAIAGAGPEGVHVLSVALSDRDSWVR
                     CRAAEALGLAGPAAAAAVPALARALSDHDYCVSGKAAESLGKVGEAALPALLEAFKDE
                     NTAVRRLAAQALTQMGPAVQRRAAEELLPALRGGDEFERGEAAQRLTSMGPAAVAVFL
                     DLLWDKDSDLRHRALDGLAEVGDSSPAVVDALAGLFRDSESTLRLRAAMVLGKMGQKN
                     PGVLARLTPLLKSPELETRRGAIQALGAMGTAGSPAVDALVVVMSDPADPMSEEAVEA
                     LNNIGTFESLRAAEGHTRKAYLDRKTQQDKPPAKKK"
BASE COUNT          442 a          849 c          855 g          462 t
ORIGIN      
        1 ggaagcagcc ccagcaattg tccgcccaga ctgtgcacgt gggccaccca tcggtcggcc
       61 aacagcacgt gcaggttcgt ttcgatggac acccggggtt cgggggtttt ttgcgccccg
      121 gaaaggagcg cccggtaggc ccgttgctgt tgttcctcgc ccttcaaaag ccaaagtttc
      181 tccacgcccg cgcgcaaggc cttcagatcc atgtccaccc atccccgatt ttcggacacc
      241 tcccggagcg ggaccccggt ggccttcacc cgcagggtgt ccggatcgat tttgaccgag
      301 gtgtttcccc cggacccctg ggacaaaagc gggtcgccgc ccaggcggcg gttcaaggcg
      361 acgaagtccg ccaggaaggg gtcaggcatg atttcggagc atcaactctt tgggatgggg
      421 ggaaagatag gtttggccga tcaaatagtc cacgccgtgg acccgggccg ccgatcgaag
      481 caaggtcacg ggaacgatca agggcaccaa ttcccgacgg aaatcctcga tggccccggc
      541 cagctcccgg cgttcggctt cggtggcctt gtcccggaaa taccccgcgg cgtgttgaag
      601 ggcgttcacg tgccgggacc gcgtggggag aaccgccagg gcccgcagaa aagcggaccg
      661 gtagtcggcg gtcagttcgc cccaggggcg gcctttgccg ttggccacca ggcggcccag
      721 ggtttggtag ctcgccgggt cgtgggccat gagaagaagt ttctcggccg tgtgaaagcg
      781 cacgagggcc cccagccctt tctccaaccc gcgaaggcgg tgggtggcga agacccgctc
      841 caggaaattt tcccgaaggc gggggtcgtt cagtcgccct tcgtcttcca cgggcaaatc
      901 cggccagcgc gcgagaagcg cccgggcaaa aaagcccact cccgatttgg tcggcgggcc
      961 cgaccggtgc accgacaccc gctccagtcc gcagctcggc gaccctttct taaggatgaa
     1021 cccgcagagg tcttcccggg ccagggcgtc ggcgcgtttt ccggcgtagc ggtgcatgtc
     1081 ctcggtcaaa tcccggccgg accgcggcgc caccagacgg acgtcgccgt cgacctcttt
     1141 taaatggatg gactcccggg gggtgcccag tccgatctcc atttccggac agacgggcac
     1201 gaattccacg aagcggccca ggaggtccgc cgcaaaggcg tcccgcttgt ggccgccgtc
     1261 gtaacggacc ttctggccca gaagacaggc cgaaattccg acgcggaggg gggcggtttg
     1321 tttcaccgag gtattatcca atttttgggc ggggtcccgg gccccggggt caaaatgtta
     1381 atatcacgcc atgaagcgcg cgaccccctt cgtcctcctg actcttgttt ttttcgcctg
     1441 cgccggcggg cagcccaaag tatcgaccct ggtcaagcaa ttgcggagct ccgattccgc
     1501 cgtcaagatc aaggccgcga acattctggc cggcctgggt tcccaggcgg aaccggcgct
     1561 gcccggtctt ctcacgatgt tccgggacac ccgcaaaccc ctgcacgacg ccgcggccaa
     1621 agccatcgcg ggcgcgggtc ccgagggcgt ccacgtgttg tccgtcgccc tctcggaccg
     1681 ggacagctgg gtgcgttgcc gcgccgccga agccctgggg ttggcgggcc ccgccgcggc
     1741 ggcggccgtg ccggccctgg cccgggctct gtcggaccac gactattgcg tttcgggaaa
     1801 ggccgccgag tccctgggga aagtggggga agcggccctt cccgccttgc tcgaagcctt
     1861 taaggacgag aacaccgccg tgcgccgcct ggccgcccaa gccttgaccc agatgggtcc
     1921 cgcggttcag cggcgggcgg ccgaggaact gttgccggcc cttcggggtg gcgacgagtt
     1981 tgaacgcggc gaggccgccc aacggttgac gtccatgggg cccgccgcgg tggcggtgtt
     2041 tctggatttg ctctgggaca aggactcgga tttgcgccat cgggccctgg acggcttggc
     2101 cgaggtgggg gattcgagcc ccgcggtggt ggacgccctg gcgggtttgt tccgggattc
     2161 ggaatccacc ctccgtctgc gggccgccat ggtgttgggg aaaatgggcc agaagaatcc
     2221 gggcgttttg gcccggctca ccccgcttct taaatccccc gaattggaaa cccgtcgggg
     2281 ggccattcaa gccctgggcg ccatgggcac cgccgggtca ccggccgtgg acgccctggt
     2341 ggttgtgatg tcggaccccg cggatcccat gagcgaggag gccgtcgagg ccttgaacaa
     2401 catcggcacg tttgaatccc ttcgggcggc cgaaggccat acccgcaaag cgtatctgga
     2461 ccgcaaaacc caacaggaca aaccccccgc caagaaaaaa tagatttctt caacgcaaaa
     2521 aaaacggccc ccgttttcca acgggggccg tttttttatt ttaagggagg ggctatttgc
     2581 cggagctctt ggcttcttcc tcgctcat
//
LOCUS       DAOQLS010000258         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_23824_length_2608_cov_4.352135, whole genome shotgun sequence.
ACCESSION   DAOQLS010000258 DAOQLS010000000
VERSION     DAOQLS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23824_length_2608_cov_4.352135"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /gene="rpsL"
                     /locus_tag="PK362_05720"
     CDS             complement(<1..360)
                     /gene="rpsL"
                     /locus_tag="PK362_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012307672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNC74362.1"
                     /translation="MTTTNQLVRAGREPLGYKTKAPALKSSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVKLSTGMEVTAYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIIRGPL
                     DATGVTDRRQGRSKYGAK"
     gene            complement(364..>2608)
                     /locus_tag="PK362_05725"
     CDS             complement(364..>2608)
                     /locus_tag="PK362_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNC74363.1"
                     /translation="VKRLGYRYATRAGLSISISEMHIPKLKEELVRKAKVEIKDIEKQ
                     SKAGVITDSERYNKIIDLWTHVTDQIADVMFDDMKKTELAVYNPKDAKFNSVFMMADS
                     GARGSRQQIRQLAGMRGLMQKPQKKITGAVGEIIESPIISNFREGLTVLEYFISTHGG
                     RKGLADTALKTADAGYLTRRLVDVAHDIVVSTDDCGTINGIRIGTIRSGEDIIEPLEE
                     RLVGRFTLDNVVNPMTDEPLVTPGHMITAAQAKQVAASGIDRIRIRSVLTCEAEHGVC
                     AKCYGLNLANGRLVEIGEAVGVIAAQSIGEPGTQLTLRTFHIGGTASRVIQRSQVVTE
                     KGGTVKFINFRTIKNRNGELICVSRNGVLSIRETGGGQEREHYDIPYGARLKIADGKK
                     AESGEILAEWDPYSLPIISEYEGTVKLQDVVDGITMHEERNRITGLIERVIIEHRAEQ
                     LHPSVLVMKGNKKEASYPLPVDTNIVAQDGDKVEPGDVVAKIPQEVSKTKDITGGLPR
                     VAELFEARKPRNSAVVSEIDGVVKLGQTARGTMKVTVTNEETGLARDYSIPQGKHLVV
                     YEGDRVGVGEPLTDGSVNPHDILRVRGAKEVQEFLVNEIQEVYRLQGVGMNDKHIEII
                     VRQMLSNVLITKSGDTDFLAGEVVSKVRFNRENKRVSDAKGDPADAQPILLGITKASL
                     SSDSFISAASFQETTRVLTDASATGQLDPLRGLKENVIIGHLIPTGSGFYDRARADKA
                     KAAASGR"
BASE COUNT          385 a          761 c          883 g          579 t
ORIGIN      
        1 tttggcgccg tacttcgagc gcccctgccg tcggtccgta acgcccgtgg cgtccagggg
       61 accgcggatg atgtggtagc gcacgccggg caagtccttc acgcggccgc cgcgcaccag
      121 cacgatggag tgttcctgca aattgtggcc gacgccgggg atgtaggccg tgacttccat
      181 gcccgtcgag agcttcacgc gggcgacctt gcggagggcc gagttgggct ttttgggcgt
      241 cgtcgtgtag acgcgcgtgc acaccccgcg gcgctgcggc gaggatttga gggcgggcgc
      301 cttggttttg tagccgagag gctcccggcc ggcgcgcacc aattggttcg ttgtcgtcat
      361 tgattatctc cccgaagcgg ccgctttggc cttgtcggcc cgcgcgcggt cgtaaaatcc
      421 ggatcccgtt ggaatgaggt gcccgatgat gacgttctct ttcaagcccc gcagggggtc
      481 caactgcccc gtggcggagg cgtccgtgag gacgcgggtc gtttcttgga aggaggccgc
      541 cgagatgaag gaatccgacg acagcgaggc cttcgtgatc ccgagcagaa tcggctgggc
      601 gtccgccgga tcgcccttgg cgtccgacac ccgtttgttt tcccggttga agcggacttt
      661 ggagaccact tcgccggcca ggaaatccgt gtcgccggat ttggtgatga gcacgttgga
      721 cagcatctgc cgaacgatga tttcgatgtg tttgtcgttc atgcccacgc cctgcaggcg
      781 gtacacttcc tggatttcgt tgaccaaaaa ttcctggact tccttggcgc cgcgcacgcg
      841 gagaatgtcg tgcgggttga ccgagccgtc ggtcaggggt tcgcccacgc cgacgcggtc
      901 gccttcgtac accaccaggt gcttgccctg ggggatgctg tagtcccggg ccagccccgt
      961 ttcctcgttg gtcacggtca ccttcatcgt gccgcgggcc gtctggccga gcttcacgac
     1021 gccgtcgatt tcggagacga cggccgagtt gcgcggtttg cgcgcttcaa aaagttccgc
     1081 cacgcggggc agaccgcccg tgatgtcctt ggttttggac acttcctggg gaatcttggc
     1141 cacgacgtcg cccggctcca ccttgtcgcc gtcctgggcc acgatgttcg tgtccacggg
     1201 cagggggtag ctggcttcct tcttgttgcc cttcatgacc aggaccgagg ggtgcagctg
     1261 ttcggcgcgg tgttcgatga tgacgcgttc gatcaggccc gtgatgcggt tgcgctcttc
     1321 gtgcatggtg atgccgtcca ccacgtcctg gagcttcacg gtgccttcgt actcggaaat
     1381 gatgggcagg gagtaggggt cccactcggc cagaatctcg ccggactccg ccttcttgcc
     1441 gtcggcgatc ttgaggcgcg cgccgtaggg aatgtcgtag tgctcccgtt cctggccgcc
     1501 gccggtttcg cggatggaga ggacgccgtt ccgggagacg cagatcaatt cgccgttgcg
     1561 gtttttgatg gtgcggaaat tgatgaactt gaccgtgccg cccttttcgg tgacgacttg
     1621 cgagcgctga atgacgcggc tcgccgtgcc gccgatgtgg aaggtgcgga gggtcagctg
     1681 ggtgccgggt tcgccgatgg actgggccgc gatgacgccc acggcttcgc cgatttccac
     1741 caagcggccg ttggccaggt tcagcccgta gcacttggcg cagacgccgt gctcggcttc
     1801 gcaggtcagg acggagcgga tgcggatgcg gtcgatgccg gaggccgcga cctgtttggc
     1861 ttgagccgcg gtgatcatgt ggcccggcgt gaccaggggt tcgtccgtca tggggttcac
     1921 cacgttgtcc agggtgaacc ggccgacgag gcgctcttcc aagggttcga tgatgtcttc
     1981 gcccgagcgg atggtgccga tgcggatgcc gttgatggtg ccgcaatcgt cggtcgagac
     2041 cacgatgtcg tgggccacgt ccaccagacg gcgggtgagg tagccggcgt ccgcggtttt
     2101 gagcgcggtg tcggccaagc cttttcgtcc gccgtgggtc gagatgaagt attccaacac
     2161 cgtcagccct tcgcggaagt tcgaaataat gggggactcg atgatttcac cgacggcgcc
     2221 ggtgatcttt ttctggggtt tttgcatcaa gccgcgcatg ccggccaatt gccggatctg
     2281 ctggcggctg ccccgggcgc cggagtcggc catcatgaac accgagttga acttggcgtc
     2341 cttggggttg taaacggcca gctcggtctt cttcatatcg tcgaacatga cgtcggcgat
     2401 ttggtcggtg acgtgggtcc acaagtcgat gatcttgttg tagcgttcgg aatcggtgat
     2461 gacgccggcc ttggactgct tttcgatgtc tttgatttcg actttggctt tccgaacgag
     2521 ctcttccttg agtttcggaa tgtgcatttc ggaaatggaa atcgacaacc cggcgcgggt
     2581 ggcatagcgg tagccgagcc gtttgacc
//
LOCUS       DAOQLS010000259         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24050_length_2594_cov_6.133517, whole genome shotgun sequence.
ACCESSION   DAOQLS010000259 DAOQLS010000000
VERSION     DAOQLS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24050_length_2594_cov_6.133517"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK362_05730"
     CDS             <1..651
                     /locus_tag="PK362_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74364.1"
                     /translation="DSLRHVLDIFTRDFGDITDVLQADPQTIVFACNSHRVPGGAYDY
                     LKTDQDNDAMNIATSPFNARFTGYDLDDESDDGDTRVDFRSSLKLIGDKLVQKDILNQ
                     PDPAIAGDWDSLTFSNQRVIAVNVSTFALTYYGGLAYLSPPHDDNADGVVTAVEIDSG
                     TVGGNNSGTLDLQSERDKLSILRVTLGIRHSKTSVDISTATTEIVLPLVPLKRRIP"
     gene            648..2030
                     /locus_tag="PK362_05735"
     CDS             648..2030
                     /locus_tag="PK362_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74365.1"
                     /translation="MTVRRPDRECSTRGTALLPVMLIFIILLPLILLLVKKMILHNTF
                     SFRDRAYRGSRGLASNALTDYMRQFSEDYRADFYQDYKLRRNWNYSSLSYSGVGLSST
                     TYNYRIDPVALSTSEVRKNRVFVFRNIGYFQNREELKKSQGGAIQFKSDLARFELIAF
                     NSLTINTNDPLDGRTLESVWIRGNLSFPSPVDFKINGPYAFSQFVTSGVIVVNGNISD
                     SRLKMDNNARVYHSGAANFDAGFTPSANENINYIPPELTPGKLPPTGGMAFVDPFQDL
                     TYYQSHFATSTVVPATITFTNTGVDINSGAGNVPFAIDATQGATILGNGAPLYIQNGT
                     GIYRKVLIVTVGGSSVTVNGPLEYHSNATKSSSGEVLAVLSDGDLVFKGNNDHNVVGL
                     YYVSDALRVIDAGNKVTARGSVILGIAHAGITTSDNSEFDIKIDTGLRSNLPAGLPEQ
                     PSLADLRVTN"
BASE COUNT          587 a          787 c          685 g          535 t
ORIGIN      
        1 gacagcctcc gccacgtcct cgatattttc acccgggatt tcggcgacat caccgacgtc
       61 cttcaagccg atccccagac catcgttttc gcgtgcaatt cccatcgcgt gccgggggga
      121 gcgtacgatt acctcaagac cgaccaggac aacgacgcca tgaacatcgc caccagcccc
      181 ttcaacgccc ggttcaccgg ctacgacctt gacgatgaat ccgacgacgg ggacacccga
      241 gtcgatttcc gttcgtctct taagttgatc ggcgataaac tggttcagaa ggacattctc
      301 aatcaacccg accccgcgat cgccggggat tgggactccc tgaccttcag caatcagaga
      361 gtcatcgccg tgaacgtgag cactttcgct ttgacctatt acgggggatt ggcctatctg
      421 tccccgccgc acgacgacaa cgccgatggc gtggtcactg cggtggaaat cgacagcgga
      481 acggtgggcg gcaacaacag cgggaccctg gacctgcaga gtgaacggga caaattatcg
      541 atccttcgcg tcaccctcgg gatccggcat tcgaagacca gcgtggacat atcgaccgca
      601 acgacggaaa tcgtactgcc tttggtgccg ctgaaacgga ggattccatg accgtccgcc
      661 ggcccgatcg cgagtgttca acgcggggaa cggcgcttct ccccgtgatg cttattttca
      721 taatcctgct tccgttgatc ttgcttctgg tcaaaaaaat gatcctgcac aacaccttca
      781 gtttccgcga cagagcctat cgaggctccc gtggcctcgc ctccaacgcc ttgacggact
      841 acatgcgcca gttttccgag gattatcggg cggattttta tcaagattac aaactccgcc
      901 ggaattggaa ttacagttcg ttgtcctatt cgggcgtggg cctttccagc accacctaca
      961 attaccgcat cgacccggtc gctttaagca cctccgaagt ccgaaaaaac cgcgttttcg
     1021 ttttccggaa catcgggtac tttcaaaacc gagaagaact caaaaaatcc cagggcggcg
     1081 ccatccaatt caaatccgat ttggcgcggt ttgaattgat cgccttcaac tcgctgacca
     1141 tcaacaccaa cgaccccctg gatggaagaa ccctcgaatc cgtttggatt cggggcaatc
     1201 tttcattccc ctctccggtt gactttaaaa tcaacgggcc ctacgcgttc tcccaatttg
     1261 tcacttccgg ggtcatcgtc gtcaacggaa acatcagcga cagccgcctt aaaatggaca
     1321 acaacgcccg ggtctaccat tccggagccg ccaatttcga cgcgggattc accccttcgg
     1381 caaacgagaa catcaattac atcccccccg aactcacacc ggggaaactt ccccccaccg
     1441 gggggatggc cttcgtcgac ccctttcaag atttaaccta ctaccaatcc cacttcgcca
     1501 cgtccaccgt ggttccggcc acgatcacgt ttaccaatac gggggtagac atcaacagcg
     1561 gggccggcaa cgtgcctttc gcaatcgacg cgacgcaagg ggccaccatt ctggggaatg
     1621 gcgcgccgtt gtacatccaa aacggaacgg ggatttaccg gaaggtcctg atcgtcaccg
     1681 ttggggggag ttcggtcacg gtgaacgggc cattggagta ccactccaac gccacaaaat
     1741 cttccagcgg ggaggttctg gccgttcttt ccgacggaga tctcgttttt aaaggaaaca
     1801 acgaccacaa cgtggtcggt ctctactacg tatcggacgc cctgcgggtc atcgatgcgg
     1861 ggaataaggt gaccgcacgg ggatcggtga tcctgggaat cgcccacgcg gggataacga
     1921 cgagcgacaa cagcgaattc gacataaaaa tcgacaccgg attaagaagc aacctgccgg
     1981 ccggattgcc ggaacagccc agcttggccg atctgcgcgt cacgaactga ccctttcccc
     2041 tcctccgggt cccccccgga aagccccaaa ttaattaaga aatacatccc gccaagcgct
     2101 tggagctctg gcgtttccgt tgaaatcgaa tttcttttgg gcttaggcca tttgggcctg
     2161 gggcgttcgg atgggcgcct taaaacgcgt attccgttgt tcttgtggcc catgaacctg
     2221 cacggcaggt gcggaaggac aacgggccca tccttagggc gggaggattc ccgcctcgat
     2281 tcaaccgctt gccttggggc aagcggggcg accgggaacg gtgaaggttc tcgtccaagg
     2341 ttggcgttag acaatgtccc ggcaagttgg aaggggccca attattaact ttttttaaaa
     2401 atggcagggg cggaaggaca acgggcacag ccctaggtcg ggaggattcc cgcctcgatt
     2461 caaccgcttg ccttgggcaa gcggggcgac cgcgaaccgt gaaggttctc gtccaaccgc
     2521 ccacaatcac ttttatttta aatggcaggg gcggaaggac aacgggcaca gccctaggtc
     2581 gggaggattc ccgc
//
LOCUS       DAOQLS010000260         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24168_length_2587_cov_4.571880, whole genome shotgun sequence.
ACCESSION   DAOQLS010000260 DAOQLS010000000
VERSION     DAOQLS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24168_length_2587_cov_4.571880"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="PK362_05740"
     CDS             <1..1440
                     /locus_tag="PK362_05740"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HNC74366.1"
                     /translation="RLVGTVRLETSSVDFDRFQNWLKYQAVAERLWARRGFYQTSGAF
                     GFRDQLQDSVNLTWVDPALARKQILLHASQQFIEGDVVHWFHTLHDGRTAFSNRSHAS
                     DNLLWLAWAAAEYVRLTGDETLLDEMTTYLRAENPFLPLPHNKHGWGTIYPRSTRGDT
                     VYRHSLKAIDLVLEKRMGVNGIPLIGTGDWNDGLDEIGSEGRGESVWLGFFLLYILKN
                     TLNIIEKKDGRHRRDYYLKRAQELEAALEKTWRGDRYLRAIHDDGTEIGVKDSGVWEI
                     DALTAAWAVMAGLNPERARTVFQTALTVLERENVVLLGWPALREDTKPYLGRSSHYPE
                     GVRENGMYCHGVQWLVKAARILAEKAEEDGDVAKGKEYRDASYRLWMKIAPLSHVATG
                     EIEVYGGQPNKQSADFLTTFDQGRMIWHGYTGAAGWMLRQAFEGVAGASLVGNQVVMP
                     LDLSELRGPLRLKAISRDLSDSPLRPGRP"
     gene            1870..>2587
                     /locus_tag="PK362_05745"
     CDS             1870..>2587
                     /locus_tag="PK362_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74367.1"
                     /translation="MKRLTTLGGALAVAAFALSSAQAGSVDTKTANVSVTIAGVNTIS
                     LAIKNMDGTAATEIGFANVDPGGSTWAAKGSQFVQLSVVDNINTGWELRNYTDNFNFA
                     GGILPDTTTWGYQYGGLRNVDVNGQRAGVGWMVLPDSDVAKNFQGPDAANPVDGASNG
                     YTFLKDRSDQNPPLVTDQSFDGSGGYKNVAFGSLTETRIVRPNRPGGNDVLSGKTAPF
                     FYFLGGDFKGVAPGHYTATIT"
BASE COUNT          524 a          821 c          771 g          471 t
ORIGIN      
        1 cggttggtgg gcaccgtgcg cctggaaacc tccagcgttg atttcgaccg gttccaaaac
       61 tggctcaaat accaggccgt ggccgagcgc ctttgggccc ggcgcggctt ctatcaaacc
      121 agcggcgcct tcggttttcg ggaccagctt caagactcgg tcaatctgac ctgggtggac
      181 cccgctctgg cgcgcaaaca aattcttctt cacgcctccc agcaattcat cgagggcgac
      241 gtggtgcact ggttccacac cctgcacgac gggcgaacgg ccttttccaa tcggtcccac
      301 gcctcggaca acctgctgtg gctggcctgg gcggcggcgg aatacgtgcg cctcaccggg
      361 gacgaaacgt tgttggacga aatgacgacg tacttaaggg ccgagaaccc cttcctgccc
      421 ctgccccaca acaagcacgg ctggggcacc atctatccgc gatccacccg gggcgatacg
      481 gtttaccggc atagcctgaa ggccatcgac ctggtcctcg aaaaacgcat gggagtcaac
      541 ggaatcccat tgatcggcac cggcgattgg aacgacggtt tggacgaaat cggcagcgag
      601 ggtcgggggg aaagcgtttg gctcgggttc ttcctcctct atatattgaa gaacaccctg
      661 aacatcatcg agaaaaaaga cggccgccac cgccgggatt actacctgaa acgcgcccag
      721 gaactggaag cggcgttgga aaaaacctgg cggggggacc gctatcttcg ggccattcac
      781 gacgacggga cggagatcgg cgtgaaagat tccggcgtgt gggaaatcga cgccttgacc
      841 gccgcctggg cggtcatggc cgggttgaac ccggagcggg cgcgcacggt ctttcaaacg
      901 gcgctcaccg tgttggagcg ggagaacgtt gttctcctgg gctggcccgc cttgcgggag
      961 gacacgaagc cctatctcgg gcgtagcagt cattatcccg aaggggtccg ggaaaacggc
     1021 atgtactgcc acggcgtgca gtggcttgta aaagcggcgc gaattctcgc ggagaaagcg
     1081 gaggaggacg gcgacgtcgc caagggcaag gaataccggg acgccagcta ccgcctctgg
     1141 atgaaaatcg ctcccctgtc ccacgtcgcc accggagaga ttgaggttta cggcggtcag
     1201 ccgaacaagc aatcggccga cttccttacg acctttgatc agggccggat gatctggcac
     1261 ggctacaccg gggccgccgg ctggatgctc cgccaggcct ttgaaggcgt ggccggggct
     1321 tctctcgtcg gaaaccaggt ggtgatgccc ctggatcttt cggagcttcg cgggcccctt
     1381 cggctcaagg ccatttcccg ggatttgtcg gacagccccc ttcgccccgg ccgcccatag
     1441 cctaaatccc ctcgttccaa gccccgccga aggccccgcc ttcggcgggg tttttcttta
     1501 tttcctcgtc ggaaaaaatg cctattgcgc tgttgtcaaa aaaagtgccc acgtgggcaa
     1561 cgaaatgtta cgtaattgtt acatttcgtt aaccatctct ttccagggtc gtattacaat
     1621 gtatccaaga gttcccggct cacatcgtaa tcgacaacgc attgttggag ccggcgccgg
     1681 aaacccgtca atggcgaggg gttcgattct tcgccggcgc gggacggtcc gcggcgacgc
     1741 gcaaaacaac gcgcgccggg cgcgcccggc cgatccgtcc agggcctctt caccggcctc
     1801 gaattgacgg tgccttccag cgacggttcc aaagccccgc actcatcctt gatgcatagg
     1861 aggaagcaca tgaagagact caccactctg ggaggagccc tcgcggtcgc cgcgttcgcc
     1921 ctctccagcg cgcaagcagg atcggtggac accaaaacgg ccaacgtctc cgtgaccatc
     1981 gcgggcgtca acaccatcag cctggcgatc aagaacatgg acggcaccgc cgccaccgaa
     2041 atcgggttcg ccaatgttga tccgggcggg tccacctggg cggccaaggg ctctcaattc
     2101 gtccaactga gcgtcgttga caatatcaac accggctggg aactgcgcaa ctacaccgac
     2161 aacttcaact ttgccggcgg catcctgccc gacacgacca cctggggtta ccaatacggc
     2221 ggtcttcgga acgtggatgt caatggccag cgcgcgggcg tggggtggat ggttctcccc
     2281 gactccgacg tggccaagaa cttccagggt cccgacgcgg ccaacccggt ggacggtgcg
     2341 agcaacggct acaccttcct caaggaccga agcgaccaaa accctccgct ggtcaccgat
     2401 cagtcctttg atggatcggg cggctacaaa aacgtggcgt tcggcagctt gaccgaaacc
     2461 cgcatcgttc gtcccaaccg ccccggcggc aacgacgttc tgagcgggaa aaccgcgccc
     2521 ttcttctact tcctgggcgg tgacttcaaa ggcgtggccc ccggtcatta cacggcgacc
     2581 atcacct
//
LOCUS       DAOQLS010000261         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24307_length_2578_cov_3.931431, whole genome shotgun sequence.
ACCESSION   DAOQLS010000261 DAOQLS010000000
VERSION     DAOQLS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24307_length_2578_cov_3.931431"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK362_05750"
     CDS             complement(<1..991)
                     /locus_tag="PK362_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74368.1"
                     /translation="MARRDGWLRAAVAGGATLLLSVNAGALNLKIRNVADDDAATGVS
                     FSSASGAANQYHTGLQYVEITHPDSHFRKVYVYTDNGGAFGQSKEGLINLDDAAQPAV
                     PFYWRNFPGRPDDFAPANEWAWTLMLEKSLPEFSVPATKDARALVTPDAGGISRVALA
                     FKTPAEVVRGTYVSRLVLEESSDVADINGPTVGHTPFNDVILVNIPIGVESTAEDDGS
                     VVSSTFYFRLDGTGAYQPRPTVLTPDPVNPFKWVVQTTLAPGDVHAGFMDYYFAARDN
                     FENETITPVYRANLVPEDGPVTRTMSAEGGLTTVPVGDPLLPGLTIDFPPRSLN"
     gene            complement(978..1718)
                     /locus_tag="PK362_05755"
     CDS             complement(978..1718)
                     /locus_tag="PK362_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74369.1"
                     /translation="MRLSASSLISIGFPVLVFFAGGPARALDLKPELIFLRGEEGAAV
                     STTIAVVNNSSETIRVTLEAHGPGEAPAPWLSLSPRALRVAAGARRVVRLKAVVPKAV
                     GEMWGEVWVRQRAPGELTETRVVRRVALRVAGTEVFEAQLKDIESGVEGNDVVVHAVL
                     VNTGNVTLRAKLVADLERMDGPLVRAVTDLTAQPVAPGESVRARLAAPKVGWFWTGRG
                     NLSAYYRDADGKTIQILKPIGEASRGTP"
     gene            complement(1708..>2578)
                     /locus_tag="PK362_05760"
     CDS             complement(1708..>2578)
                     /locus_tag="PK362_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74370.1"
                     /translation="AATRGLRPPGAPAGWPMRTFSFLGASALALLLAPVLRANTFLEV
                     RPGVVEESVPEGKTAKGKLIVRNTGDTEVLVEVEVRDEWATRTGLPSLPPSEWLDLKY
                     PHPFVLKPGAAKEVGYTVTPPTSFNGEAMAMVVFGGPTSAGSGGTGYRFRQGIPFYML
                     STTTVQGQIALASLQVYLNAIKQLEFAVELKNNGPVHVRAQGALKISNSRGAEIGNAP
                     LAGGIPVFPTKTQKYYARDQKNNYSEGLYRGELSVAGEKGEILLVGVCGFRIDKKGQI
                     TITSPFAERPRAP"
BASE COUNT          478 a          816 c          823 g          461 t
ORIGIN      
        1 cgttcaggct gcggggaggg aaatcgatgg tcaggcccgg caggagggga tcgcccacgg
       61 gcaccgtggt gaggccgccc tccgccgaca tcgtccgcgt cacgggcccg tcctcgggga
      121 ccaggttggc ccggtagacg ggcgtgatcg tttcgttttc gaaattgtcc cgggcagcga
      181 aataatagtc catgaatccg gcgtggacgt cccccggcgc cagggtcgtc tggaccaccc
      241 atttgaaggg gttgaccgga tccggcgtca acaccgtcgg ccgcggctgg taggcccccg
      301 tcccgtcgag ccggaaataa aaggtggacg acaccacgga cccgtcgtct tccgccgtcg
      361 actcgacgcc gatggggatg ttgaccagga tcacgtcgtt gaacggcgtg tggccgaccg
      421 tggggccgtt gatgtcggcg acgtcgctgg actcctccag cacgaggcgg gaaacgtagg
      481 taccccgcac cacttccgcc ggggttttaa aggccagggc cacgcgggaa attccgccgg
      541 cgtccggggt cacgagggcc cgggcgtcct tggtggcggg aaccgaaaat tcggggaggg
      601 atttttccag catcaatgtc caggcccatt cgttggccgg cgcgaagtcg tcgggccggc
      661 cggggaaatt gcgccaatag aagggaacgg cgggttgggc cgcgtcgtcc aaattgatga
      721 gcccttcctt ggattgtccg aaagcgccgc cgttatcggt gtacacgtac acttttcgaa
      781 agtgggaatc ggggtgggtg atttcaacgt attggagccc ggtgtggtac tggttggccg
      841 ctcccgacgc cgagctgaac gacacgcccg tggcggcgtc gtcgtcggcc acgttgcgga
      901 ttttcaaatt caacgcgccc gcgttaacgg acaacaagag ggtcgcgccg cccgcgaccg
      961 ccgcgcgaag ccagccgtca cggcgtgcca cgcgacgcct ccccgatggg tttaaggatc
     1021 tggatggttt tgccgtcggc gtcccggtaa taggccgaga gattcccgcg ccccgtccaa
     1081 aaccatccga ctttgggggc ggccaaccgg gcccggacac tttccccggg ggcgacgggt
     1141 tgggcggtca ggtccgtcac cgctcgaacc aggggaccgt ccattcgttc caaatcggcc
     1201 accaacttgg cgcgaagggt cacgttgccc gtgttgacca acaccgcgtg aaccaccacg
     1261 tcgttgcctt ccaccccgga ttcgatgtct ttgagttggg cctcaaacac ctcggtgccg
     1321 gccacgcgca gggccacgcg ccgcaccacc cgggtttcgg tcaattctcc gggggcccgc
     1381 tgacgaaccc aaacctcgcc ccacatttca ccgaccgcct tgggaacgac ggccttcagc
     1441 cggaccacac ggcgggcccc cgcggccacc cgcagggccc ggggactcag ggacaaccac
     1501 ggcgccggcg cctcgcccgg cccgtgggcc tccaacgtga cccggattgt ttccgaggag
     1561 ttgttgacga ccgcgatggt cgtggaaaca gcggcgcctt cttccccgcg gaggaaaatc
     1621 aattccggct taagatccag cgcgcgcgcc gggccgccgg caaaaaacac caacaccggg
     1681 aacccgattg aaatcaaaga cgatgcgcta aggcgcacgg ggtctctccg caaaagggct
     1741 ggtaatggtg atttgccctt tcttatcgat acgaaaaccg cacacgccga ccaaaagaat
     1801 ttcacccttc tcgccggcca cgctcaattc gccgcggtaa agtccctcgg aatagttgtt
     1861 tttttgatcc cgcgcataat atttttgcgt cttggtgggg aaaaccggta tgccgccggc
     1921 cagcggcgcg ttgccgattt ccgctccgcg ggagtttgaa attttcagtg ccccttgagc
     1981 gcggacatga acgggcccat tgtttttcag ttccacggca aattccaact gcttgatcgc
     2041 gttgagatac acctgaaggg aggccagggc tatttggccc tgaacggtcg tcgtcgacaa
     2101 catgtaaaag ggaattcctt gccgaaatcg atatccggtg ccgccgctgc cggcggaggt
     2161 gggtccgcca aaaacgacca tggccatggc ttcgccgtta aacgaggtcg gcggcgtgac
     2221 ggtgtaaccc acttctttcg cggcgccggg ttttaaaaca aaggggtggg gatatttcag
     2281 gtccagccat tcggagggcg gaagagacgg aaggcccgtg cgcgtggccc attcgtcccg
     2341 cacttcaact tcaacgagga cttcggtgtc gccggtgttg cggacgatca attttccttt
     2401 ggccgtcttg ccctcgggaa cggactcttc cacgaccccc ggccgcacct ccaaaaaggt
     2461 gttggcccgg agaaccgggg caagcagaag cgccagcgcg gaagcgccga ggaacgagaa
     2521 tgtccgcata ggccaccccg cgggcgcgcc cgggggccga agcccccggg tcgccgcc
//
LOCUS       DAOQLS010000262         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24353_length_2576_cov_2.746132, whole genome shotgun sequence.
ACCESSION   DAOQLS010000262 DAOQLS010000000
VERSION     DAOQLS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24353_length_2576_cov_2.746132"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..821
                     /locus_tag="PK362_05765"
     CDS             18..821
                     /locus_tag="PK362_05765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC74371.1"
                     /translation="MTDDTVEAAPPPHIFEDVIGLRLAAPADDWRNRPDMSPFTRPFR
                     ASILARARFVEDLLAEEAARGLGQYVLRGAGLDTFAQRRKDLASRLVVFEIDRPGPQA
                     WKRQRLIDLGFGLPPFLRLVPVDFEAGEAWQDKLAAAGFDARRSALLASTGVSMYLTR
                     EANIDTLRRVASFAAGSTLVLSFMLPIEMADPEIRPGIERAAAGARANGTPFLSFFTP
                     GDLLSLARDAGFKDVRHVPAAALAERYFSGRTDGLRPPNNAEELLVAST"
     gene            943..1377
                     /locus_tag="PK362_05770"
     CDS             943..1377
                     /locus_tag="PK362_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNC74372.1"
                     /translation="MAFEHSRKIAASPEEVFAAFADGKRLARWWGPAGFTNTFTTFDF
                     QPGGKWSFTMHGPDGKDYPNEMIVETIERNRKIVFHHVCAPLFLLTTTLEPTSGGGTT
                     VRWKQEFEDPEFGRRMAQILVPANEQNLDRLSAEVLGPRAGR"
     gene            1425..1994
                     /locus_tag="PK362_05775"
     CDS             1425..1994
                     /locus_tag="PK362_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC74373.1"
                     /translation="MASAFSHAVIATALGKTILPRESPRWTWALGAVLAAVPDLDVVG
                     FHFGIRYGDTFGHRGFTHSFFFAAVVAAFLAVALQRRPRVSGRRLFGFFFLALASHGL
                     ADALTNGGLGIAFFAPFSNARYFFPFRPVAVSPIGLERFFSSRGGAVLRSEAIWLVLP
                     SLGVFAAATIARGIERERRTGGTRKSGGL"
     gene            2042..2305
                     /locus_tag="PK362_05780"
     CDS             2042..2305
                     /locus_tag="PK362_05780"
                     /inference="COORDINATES: protein motif:HMM:NF026967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HNC74374.1"
                     /translation="MAKDTAGDAKPTAGVVPDEKTAIRIALAVWEPLYGTKDVGGKAP
                     YRARLEGNVWRVEGTLPPGHLGGVPTAEISKVDGRILRVSHGQ"
BASE COUNT          439 a          829 c          806 g          502 t
ORIGIN      
        1 gttaccccgc cgtcgccgtg accgacgaca ccgtggaagc cgcccccccg ccccatattt
       61 tcgaagacgt catcgggttg cggttggccg ccccggccga cgattggcga aaccgcccgg
      121 acatgagccc cttcacccgc cccttccgcg cctccatcct cgcccgcgcc cgttttgtgg
      181 aggatctcct ggcggaagag gcggcgcggg gcctggggca atacgttctc cggggggccg
      241 gcctggacac cttcgcccaa cggcggaaag acctggcctc ccgcctcgtc gttttcgaaa
      301 tcgatcgacc gggtccccag gcctggaaac ggcaacggct gatcgacctg ggcttcggcc
      361 ttcccccctt tcttcgcctc gttcccgtgg atttcgaggc gggggaggcc tggcaagaca
      421 aattggccgc cgccggattt gacgcccggc ggtccgccct cctggcgtcc acgggggtga
      481 gcatgtacct cacccgggaa gccaacatcg acacgctgcg gcgggtcgcg agcttcgcgg
      541 ccggctccac gttggtcctt tccttcatgc tccccatcga aatggcggac cccgagatac
      601 gccccggcat cgaacgggcc gccgcggggg cccgggccaa cggcaccccg tttctcagct
      661 ttttcacccc cggggacctt ctgtccctgg cccgcgacgc cggttttaag gacgtccggc
      721 acgtgccggc cgccgctctg gccgaacggt atttcagcgg ccgaaccgac gggctccgcc
      781 cgccgaacaa cgccgaagaa ctgctggtcg cctccacctg acccgcgccg acgcccgtca
      841 aaagattcac cggtccattg cccgggggat ggtaaaatga ggggcgatcg atgggcgccc
      901 cgggacaacg accccttcga atcgccaagg agcgaccggg caatggcttt tgaacattca
      961 cgcaaaatcg ccgcttctcc cgaagaagtc tttgccgcct ttgcggatgg gaaacgcctg
     1021 gcccgttggt gggggccggc cggctttacg aacactttca cgacctttga tttccaaccc
     1081 ggcggaaagt ggtccttcac gatgcacggg cccgacggga aggactatcc caatgaaatg
     1141 atcgtggaaa caatcgaacg gaaccgtaaa attgtgtttc accacgtttg cgctccgttg
     1201 ttcctcttaa cgacgaccct ggaacccacc agcggcgggg gaaccaccgt acgctggaaa
     1261 caggaattcg aagaccccga attcgggcgc cgcatggctc aaatattggt cccggccaac
     1321 gagcaaaatc tggatcggct gtcggcggaa gtgctgggcc cccgtgcggg tcgataattt
     1381 ccgcccgccg gcggcccccg ggtcctcggg ccaaagggaa taaattggcg tcggcctttt
     1441 cccacgcggt gatcgccacg gccctcggca agacgattct gcccagggaa agcccccgtt
     1501 ggacctgggc cttgggcgcg gtcctggcgg ccgttccgga cctggacgtg gtggggtttc
     1561 acttcggcat tcggtacggc gacaccttcg gccatcgagg ctttacccat tcttttttct
     1621 ttgcggcggt ggtcgccgct tttttagcgg tcgccctcca acgtcgcccc cgggtttcgg
     1681 gccgacgtct cttcggtttt ttctttctgg cgctggcctc ccacgggttg gcggacgccc
     1741 tgaccaacgg gggactcggg atcgcttttt ttgcgccctt ttccaacgcc cggtattttt
     1801 ttccctttcg ccccgtcgct gtttccccca tcggcctgga acggtttttt tcctcccggg
     1861 gaggggcggt tcttcgaagc gaagcgattt ggttggtcct tccgtccctg ggtgttttcg
     1921 ccgcggcgac aatcgcccgt ggaattgaac gggagcgccg aacgggcggg acaaggaaaa
     1981 gcggaggctt atgaggaacg cggtttttgt tttggcattt attttcggaa tcggaggtct
     2041 tatggcgaag gacaccgcgg gcgacgccaa accgacggcg ggggtggtcc cggatgaaaa
     2101 aacggccatt cgcattgccc tggccgtttg ggaaccgctc tacggaacga aggacgtggg
     2161 gggaaaggcg ccctatcggg cccgcctcga gggaaatgtc tggcgggtgg aaggcaccct
     2221 tcccccggga catctcgggg gggttccgac ggccgagatt tcaaaagtgg acggtcgaat
     2281 tctccgagtg agccatggac aataattttc ttcccgtcga ggcggggccg ggcggggcgt
     2341 ggatcgcccc ggaaaccgaa aaatccgccg gtcaacgacc gtgaacaaaa ttgtttttgt
     2401 caaaacccct tcgggccccg tgaccgtcgg cgatttttgg gccgaccggc cgccctgggt
     2461 tcaaaaaggt ctgggggtcg cggcggggga actgtcggcg tccaatcaaa tcaaaagcgt
     2521 tcgcgcccac gagcgtaaaa acggcgagtg gtccgtctgg ctcgaggacg aaaaag
//
LOCUS       DAOQLS010000263         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24385_length_2574_cov_3.421993, whole genome shotgun sequence.
ACCESSION   DAOQLS010000263 DAOQLS010000000
VERSION     DAOQLS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24385_length_2574_cov_3.421993"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PK362_05785"
     CDS             complement(<1..1111)
                     /locus_tag="PK362_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNC74375.1"
                     /translation="MAKGPWAVTGPWAPNQTPEGIRGRYLRSFLVLGVLLGALFGLTL
                     LIGEPARLFALGYEFLTAQVAKHGLPAWIPSLVALVVPCSIMLGLIQVLPIFLVWWEM
                     KIAAHIQVRLGPMRVGWWHGALQTIADGMKLLFKEDIVPDGADRVLHFLAPVIVVAPA
                     YLCFAPIPFGNGSLSAVNLDVGLLFILGVSGLTVIGLLMAGWSSNNKYSVLGGLRAAA
                     QMVSYEIPRVIAVLPVVMWAGTLSLGGLMDAQKGLWMGFLPRWFIFYPVIGQIAFIVY
                     IICSIAETNRTPFDIAEAESELTSGFHTEYSGMKWAMFFMAEYAYMFLASALGTVLFL
                     GGGSAPWPFLDFVPSFVWFFAKSFFIIFLFIWFRWS"
     gene            complement(1111..2220)
                     /locus_tag="PK362_05790"
     CDS             complement(1111..2220)
                     /locus_tag="PK362_05790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNC74376.1"
                     /translation="MERLSEHEMLLNMGPQHPSTHGVLRVVLRLDGEKVTGAVPDLGY
                     LHRGVEKLAENRTYPQFIILTDRDDYLCAMLNNWAYCLTVEKLMKVEIPERAQYLRVI
                     AGELNRIASHLLFIGTFGIDIGAFTPFLYAFGREREMILDLFEQLCGARITYNYVRIG
                     GVSNDVPEGWIEKAKKFVAHMKTCLKEYDDLLSYNPIFMDRTKGIGVLSKDKAIAYGV
                     TGPNLRASGVNLDLRKSAPYGIYAKFQFDAAVRTNGDCWDRYMVRRDEIEQSCRILEQ
                     ALEGFPAGEILGKVPKANLRPPAGEAYGQLEGARGILGVYLVSDGGLSPYRLHLRAPS
                     YINLATLQEILVGWKVADVIAILGSIDIVLGEVDR"
     gene            complement(2301..>2574)
                     /locus_tag="PK362_05795"
     CDS             complement(2301..>2574)
                     /locus_tag="PK362_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011363236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNC74377.1"
                     /translation="FVKVDLPREGEPALPSITPLFAAADWQERETYDLFGIRFTGHPN
                     LRRILLWEGYGGWPLRKDYVHTADKYDNGSEIGLPKAAPAAHGAAQ"
BASE COUNT          573 a          738 c          807 g          456 t
ORIGIN      
        1 aggaccaacg gaaccagatg aagaggaaga tgatgaaaaa gcttttggcg aaaaaccaaa
       61 caaaggaagg aacgaagtcc aggaagggcc agggggccga accgccgccc agaaacaaca
      121 cggtgcccag ggccgacgcc aagaacatgt aggcgtattc cgccatgaag aacatggccc
      181 atttcatccc cgaatattcg gtgtggaacc ccgaggtcaa ttccgattcg gcttccgcga
      241 tgtcgaaggg cgttcggttg gtctcggcga tggagcagat gatgtagacg atgaaggcga
      301 tttggccaat aacgggatag aaaataaacc agcgtggcaa gaatcccatc cagagcccct
      361 tttgggcgtc cattagcccc cccagggaaa gggtcccggc ccacatcacc acgggcaata
      421 cggcgatgac ccgggggatt tcataggaga ccatctgcgc cgcggcccga agaccgccca
      481 agaccgagta tttattgttg gagctccacc ccgccatcaa aaggccgatc acggtgaggc
      541 ccgaaacccc caggatgaaa agcaggccca cgtccaagtt gaccgcggac aggctcccgt
      601 tgccgaaagg aatgggcgcg aagcagagat aggccggcgc cacgacgatc accggcgcca
      661 ggaaatgcaa gacgcggtcc gccccgtcgg ggacgatgtc ttccttgaac aacaacttca
      721 tcccatcggc gatggtttga agcgccccgt gccaccaacc gacccgcatg gggcccagcc
      781 gcacctgaat gtgggccgcg attttcattt cccaccaaac gaggaaaatg ggaagaactt
      841 gaatgaggcc cagcatgatg gaacagggga ccaccagggc caccagggaa ggaatccagg
      901 ccggaagtcc gtgtttggcc acctgggccg tcaaaaattc atatcccagg gcaaagaggc
      961 gcgccggttc accaataagc agggtcaagc cgaacaaagc gcccaaaagg acgccgagaa
     1021 cgaggaacga gcgaaggtaa cgcccccgaa tcccttcggg ggtttgattg ggggcccagg
     1081 gccccgtgac cgcccacggt cccttggcca ttagcggtcc acctcgccca aaacgatgtc
     1141 aatggaaccc aaaatggcga tcacgtccgc gactttccac cccaccaaaa tctcttgaag
     1201 ggtggccaaa ttgatgtagg acggggcccg gaggtgaagg cggtaggggc tcagcccccc
     1261 atcggacacg aggtagaccc ccagaattcc ccgggcgcct tccaactggc cgtaggcttc
     1321 gccggcgggc gggcggaggt tggccttggg cactttcccc aggatttccc ccgccggaaa
     1381 gccctccagg gcctgttcca aaatccggca ggattgttcg atttcgtccc ggcgaaccat
     1441 gtaccggtcc caacagtcgc cgttcgttcg cacggcggcg tcaaattgga atttggcgta
     1501 gatcccgtag ggcgcgctct tgcggaggtc caggttgacg ccgctggccc gtaggttggg
     1561 ccccgtgacg ccgtaggcga tggctttatc cttggagagg acgccgatgc ccttggtgcg
     1621 gtccataaaa atggggttgt agctcaacaa atcgtcgtat tctttgaggc aggttttcat
     1681 gtgcgccacg aacttcttgg ccttttcgat ccaaccttcg ggaacgtcgt tggaaacgcc
     1741 gccgatgcgc acgtagttgt aggtgatgcg ggcgccgcaa agctgttcaa agaggtccag
     1801 gatcatttcc cgttcccgcc cgaaggcgta caggaagggc gtgaaagccc cgatgtcgat
     1861 cccgaaggtt ccaatgaaaa gcaggtggga ggcgatccgg ttcaattcgc ccgcgatcac
     1921 gcggagatac tgggcccgct ccggaatttc gactttcatc agtttctcaa cggtcaggca
     1981 gtaggcccag ttgttgagca tggcgcagag atagtcgtcc cggtccgtca aaatgatgaa
     2041 ttgggggtag gttcgatttt cggcgagttt ttcgaccccc cgatggaggt agcccagatc
     2101 gggcaccgcg ccggtgactt tttccccgtc caaacggaga acgacgcgca aaacgccgtg
     2161 ggtcgaggga tgctgggggc ccatgttgag cagcatctcg tgttcggaga gccgctccat
     2221 ctttgtttca aaagaggggg ccggtaaaag ggcgtcggcg cgctcgttga tctggcccgc
     2281 ggggccggcg aggggcgtcg tcattgggcg gctccgtggg cggcgggcgc ggctttgggc
     2341 aagccgattt ccgagccgtt gtcgtatttg tcggcggtgt gaacgtaatc cttgcgaagg
     2401 ggccacccgc cgtagccctc ccacagtaaa atgcgccgga ggttggggtg gccggtgaaa
     2461 cggattccaa ataaatcgta ggtttcccgt tcctgccaat cggccgccgc gaagaggggc
     2521 gtgatggagg gaagcgccgg ttcgccctca cggggcaggt cgactttgac aaag
//
LOCUS       DAOQLS010000264         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24433_length_2571_cov_4.056439, whole genome shotgun sequence.
ACCESSION   DAOQLS010000264 DAOQLS010000000
VERSION     DAOQLS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24433_length_2571_cov_4.056439"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK362_05800"
     CDS             complement(<1..297)
                     /locus_tag="PK362_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNC74378.1"
                     /translation="MPLTTLLGKKAVDALAGNISLVYPDFKAERFRQWALDGLEPLGI
                     LERGKHLARALRACLPERFEDAAKILLASLTPPLSETEDLGLAVFFYLPHVCFVA"
     gene            complement(393..968)
                     /locus_tag="PK362_05805"
     CDS             complement(393..968)
                     /locus_tag="PK362_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74379.1"
                     /translation="MGGAVGARTPPTVLESIDRLERALAQDDDREPTLSAVDPLLGER
                     EAQAKKLAALRVGGKAVPPAVVYFCSDRESLDCPGLYADDTIHLYEKDFEPQFAALKA
                     PIGFRISEELKKPAVAFLGNAGELHERGYKALRKVSIENTGRVGLAGAVGRGNQVLLV
                     FAETPDRRRRFKFVWLVDGSTIAPRSSPRLK"
     gene            complement(1075..1431)
                     /locus_tag="PK362_05810"
     CDS             complement(1075..1431)
                     /locus_tag="PK362_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNC74380.1"
                     /translation="MKIPMTLLTVVLFLTLLGGLFLVFRATLGESVPGEARGAAERLK
                     KVGRNEPCFCGSGRKYKTCHRDADRDLKGRLDAQADHDAVAAGNEPLIDREVARRGFS
                     KWPGIFRRGRSGGGSS"
     gene            1520..2122
                     /locus_tag="PK362_05815"
     CDS             1520..2122
                     /locus_tag="PK362_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74381.1"
                     /translation="MDAIASHIHDALTQVRELRRNILAKQRFKGYSGRARILSGSLAL
                     GAAALIQTLPPSKYTVLFGWLAVLALGLFINYGALLYWFLFDPEVRRDVTKLKPVLRA
                     VPSLAVGGVLTLAMLRSRIFWPLYGVWMTLFGLANLTGEDVLPSRILYVGLFYLVCGT
                     VYLMSPSPTFLNPWPMGIVFFTGEWAAGLVMHFDREETQP"
     gene            2119..2487
                     /locus_tag="PK362_05820"
     CDS             2119..2487
                     /locus_tag="PK362_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNC74382.1"
                     /translation="MTNPFDEIKKVFHEPNRLAIMSALLRAPEGLSFADLKAACRLTD
                     GNLSRHLKALADLNALKMKKSFVGVKPRTTVYVTERGRDHFLRYLAALEEVLHAAQSA
                     RSKPADRHADGPDGATAAAG"
BASE COUNT          502 a          818 c          723 g          528 t
ORIGIN      
        1 cgccacgaag cacacgtggg ggagataaaa aaacacggcc aggcccaggt cctcggtctc
       61 cgacagcggc ggcgtgaggg aggccaacag aattttcgcg gcgtcctcga accgctccgg
      121 caaacaggcc cgcagagccc gggccaaatg cttgccgcgt tccagaatcc ccaagggctc
      181 caacccgtcc agagcccatt ggcgaaaccg ctcggcctta aaatccgggt aaaccaggga
      241 aatatttcca gcgagggcgt ccacggcctt tttccccaac agcgtcgtca agggcacccc
      301 tttttgaatc gacttcgggg cggcgggaat tttaaattcg gggaaggcat tccccctcgg
      361 tttcgcccga gcaagcgccg gagaaggttt tttcatttta gccgggggga ggatcgcggg
      421 gcgatggtcg atccgtcgac gagccacaca aacttgaacc gccgtcgacg gtccggggtc
      481 tccgcgaaaa ccagaagaac ctgattcccc cgacccacgg ctcccgcgag ccccacccga
      541 ccggtgtttt caatggaaac cttccgaagg gccttgtatc cccgttcgtg aagttccccg
      601 gcgttcccca gaaaggcgac cgccggcttt ttcaactcct cggaaatccg gaacccgatc
      661 ggagctttta acgccgcaaa ttggggctcg aaatccttct cgtacagatg aatcgtgtcg
      721 tcggcgtata agccggggca atccagggat tctcgatcgg aacagaaata aacgacggcg
      781 gggggaaccg ctttgccccc cacgcgcaac gccgccaatt ttttggcttg ggcttctctt
      841 tcccccaaga gaggatcgac ggcgctcagg gtgggttcgc gatcgtcgtc ctgggccagg
      901 gccctctcca atcgatcgat ggattccagg acggtcggcg gggtacgggc tcccaccgcc
      961 ccccccattg tcaggcccag gatcgccccc ccgaaaagcg tccatcgtgc gatcgggttc
     1021 gcggaccgcc gcccatcgcc ggggatcccc gcgttcgttg gaagccggaa cggattaact
     1081 ggaacccccg cccgatcggc cccgtcgaaa tattccgggc catttcgaga atccccgccg
     1141 ggcgacctcc cggtcgatga gcggctcgtt gccggcggcg acggcgtcgt ggtccgcctg
     1201 ggcgtccagt cggcctttca aatcccggtc cgcgtcccgg tggcaagtct tgtatttccg
     1261 cccgcttccg caaaaacagg gctcgtttcg tccgactttt ttaagacgct cggccgcgcc
     1321 ccgggcctcc ccggggacgg attcgcccag ggtggcgcgg aagaccagga aaagaccccc
     1381 cagaagggtc aagaatagaa cgacggttaa tagcgtcatc ggtattttca ttctaacacg
     1441 aaagagccgg agacgccgac tatccggtca caaactttct attgcaaagt tctttgtttc
     1501 atgctatcct aaaatcgcca tggacgccat cgcctcccac attcacgacg cgctgaccca
     1561 ggtgcgggaa cttcgccgga acattttggc gaagcaacga ttcaaaggct attccggccg
     1621 ggcccggatc ctttccggct ccctggccct cggggccgcc gccttgattc aaacgcttcc
     1681 tccctccaaa tacacggtcc ttttcggctg gctggcggtc ctggccctgg gccttttcat
     1741 caattacgga gcgcttctct attggtttct ctttgatccg gaagttcggc gggacgtaac
     1801 gaaactcaaa ccggtcctgc gcgccgttcc gtccctggcc gtgggcggcg tgctgaccct
     1861 ggccatgctg cggtcccgaa tattttggcc gctctacggc gtttggatga cccttttcgg
     1921 gctggccaat ttaaccgggg aagacgttct cccctcccgc atcctttatg tgggcctttt
     1981 ttacctggtc tgtggaacgg tttacctgat gtccccctcg ccgacctttt tgaatccctg
     2041 gcccatgggc atcgtttttt tcaccgggga atgggccgcg gggctcgtga tgcattttga
     2101 tcgggaggaa actcagccgt gaccaacccc ttcgacgaaa ttaaaaaagt gtttcacgaa
     2161 cccaaccggc tggccatcat gtcggccctg ttgcgggctc cggaaggcct gtcttttgcg
     2221 gacctgaagg ccgcctgccg attgaccgac ggcaacctga gccgccatct caaagccctg
     2281 gccgatttga acgccttaaa aatgaagaaa tccttcgtgg gagtaaaacc ccgcacgacg
     2341 gtttatgtga ccgaacgggg gcgggatcat tttttgcgct acctggcggc cttggaggaa
     2401 gtccttcacg cggcccaatc cgcccgctcc aaacccgccg atcgacacgc cgacggaccc
     2461 gacggagcca cggccgcggc gggataattt ttttggtcat aagctttgca ttgcaaagaa
     2521 ctttacataa aacgaccgag gaggcgttat gaaaaacatc gacgtgctgg t
//
LOCUS       DAOQLS010000265         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24435_length_2571_cov_3.566375, whole genome shotgun sequence.
ACCESSION   DAOQLS010000265 DAOQLS010000000
VERSION     DAOQLS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24435_length_2571_cov_3.566375"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1513)
                     /locus_tag="PK362_05825"
     CDS             complement(197..1513)
                     /locus_tag="PK362_05825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNC74383.1"
                     /translation="MKPKHVAVVGTGYVGLVTGACLADIGHRVVCVDADKAKVATLKK
                     GGLPIYEPGLDAVVAANRKKGRLSFTGDLKEAMKTAELVFIAVNTPPLPNGEADLSFV
                     ETVARQVAQNLHRYTVIVEKSTVPVNTGAKVRQTLRLHGKKGVEFDVVSNPEFLREGT
                     AVQDFSKPDRIVVGVESERAEKVMRELYAPIKAPFVVTDIKSAELIKHASNSFLATKI
                     SFINAVAALCERVGADVSLVAKGMGYDARIGASFLRAGLGYGGSCFPKDVSAFIKMAE
                     GVGVDFKLLKAVREINDHQRHWAVERLRQALWNLRGKTVAVWGLAFKPDTDDLRNAPA
                     LDIIHALQAEGCKVTAHDPVAMGKAGPLLPGVRLVKTPLEAVREADALLVATEWKEYK
                     AIELSRVKALLRTPVLVDGRNLFDPAAAAALGFLYHSVGRSSQPAK"
     gene            complement(1510..2535)
                     /gene="rfbB"
                     /locus_tag="PK362_05830"
     CDS             complement(1510..2535)
                     /gene="rfbB"
                     /locus_tag="PK362_05830"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNC74384.1"
                     /translation="MKILVTGGAGFIGSHFVNHWLEHHRNDRIVTLDKLTYAGDRSNL
                     GDALRSPRHRFLKGDIGRRGDVDRALVGVEAVVNFAAETHVDRSLLDAAPFLRTNVEG
                     THVLVNAARLAGVRRFLHVSTDEVYGSVEKGRSPESAALNPTSPYAASKAASDHLVRA
                     QWLTHGFPTIVTRCTNNFGPRQHPEKFLPLFITNALDGRPLPLYGKGDNVRNWIHVRD
                     HCEALDRVLRRGRPGSIYNVAADREHRNIDVARAILKAVGAPAALLAFVKDRPGHDRR
                     YAPDAGKIRRELGWRARRPFERELPGLIEWYRSQEPWWRRVKDRSFGAYYQKQYGGRL
                     PKNKEKS"
BASE COUNT          454 a          826 c          808 g          483 t
ORIGIN      
        1 tggaacctcc ttcttccgat aaatggggtt taaaatcgag aaagaacttt tcacccccaa
       61 ctctggaagg gaaggaataa ggaacgccgt attaacgagg ggtttttctt tggacgtcat
      121 ggtctcagaa ccttagacac tacgatgctc ttaattgtaa tttttacacg gaccgccatc
      181 aagcgcaggg ggggtcctat ttggccggct gcgacgagcg gcccacgctg tggtaaagga
      241 aacccagggc cgcggcggcg gccgggtcga agaggtttcg accgtcgacg agcaccggcg
      301 tgcgcagaag ggctttgacc cgggaaagtt cgatggcttt gtactctttc cactcggtgg
      361 cgaccaggag ggcgtcggct tcccgcacgg cctccagggg ggtcttcacc agacgaaccc
      421 ccggcagaag cgggccggcc tttcccatgg ccacagggtc gtgggcggtc accttgcacc
      481 cctcggcctg gagggcgtga atgatgtcca aggccggggc gttccgcaaa tcgtcggtgt
      541 ccggcttaaa cgccaggccc cagacggcca cggtttttcc ccgcaaattc cagagagcct
      601 gacggagtcg ctccaccgcc caatggcgct ggtggtcgtt gatctcccgc accgccttga
      661 gcagcttgaa atccaccccc accccttcgg ccatcttgat gaaggcgctc acgtccttgg
      721 gaaagcagga ccccccgtag cccaaaccgg cccggaggaa agaggccccg atgcgcgcgt
      781 cgtagcccat ccccttggcc accagggaca cgtcggcccc cacccgctcg caaagggcgg
      841 ccacggcgtt gatgaaggag attttggtcg ccagaaaaga attggaggcg tgcttgatca
      901 gctccgcgct tttgatgtcg gtcacgacaa aaggcgcttt gatgggggcg taaagctccc
      961 gcatcacttt ttccgcccgc tcggactcga cgccgaccac gatgcggtcg ggtttggaga
     1021 aatcctggac ggcggtgcct tcccgcagga actccgggtt ggacaccacg tcgaactcca
     1081 cgcccttttt gccgtgcaag cggagggttt ggcggacctt ggcgcccgtg ttgaccggca
     1141 ccgtggattt ctccacgatg acggtgtagc ggtggaggtt ttgcgccacc tggcgggcca
     1201 cggtctccac gaaggacaaa tcggcctctc cgttggggag cggcggggtg ttcacggcga
     1261 tgaaaaccaa ttccgccgtt ttcatggcct cttttaaatc gccggtgaag gagaggcggc
     1321 cctttttgcg gttcgcggcc accacggcgt ccaagcccgg ctcgtagatg ggcaggccgc
     1381 cctttttcaa cgtcgccact ttggccttgt ccgcgtcgac gcagacgacc cgatgtccga
     1441 tgtccgccag gcaggcgccc gtgaccaggc ccacgtatcc cgtgccgacc accgctacgt
     1501 gtttgggttt catgacttct ccttgttctt ggggaggcgg ccgccgtatt gtttttggta
     1561 ataggcgccg aaggaccggt ccttgacgcg ccgccaccag ggttcctggg accggtacca
     1621 ttcgatcaaa ccgggcaatt cccgctcaaa gggccggcga gcccgccagc ccagttcccg
     1681 ccgaatcttc ccggcgtcgg gcgcgtaccg gcggtcgtgc cccggccggt ccttcacgaa
     1741 ggccaacagg gcggcgggag cgcccacggc cttcaagatc gcccgggcca cgtcgatgtt
     1801 tcggtgctcc cggtccgcgg cgacgttgta aatcgacccc ggccggcccc gccggagcac
     1861 ccggtccagg gcctcgcaat ggtcccgcac gtgaatccag ttccggacgt tgtcgccctt
     1921 cccgtagagc gggagcgggc gcccgtccag ggcgttggtg atgaaaagcg gcaaaaactt
     1981 ttccgggtgc tgtcgcgggc cgaaattgtt cgtgcaccgg gtgacgatcg tcggaaaccc
     2041 gtgggtgagc cactgggccc gcaccaggtg gtccgaggcg gccttggacg cggcgtaggg
     2101 gctcgtcggg ttcaacgccg ccgattcggg gctgcgcccc ttttccacgg aaccgtagac
     2161 ctcgtccgtc gagacgtgca aaaagcgtct cacgccggcg agccgggccg cgttcaccag
     2221 aacgtgggtg ccctcgacgt tggtgcgcag aaaaggggcg gcgtccaaca gggaccggtc
     2281 cacgtgggtt tcggcggcga agttcaccac cgcctccacc cccaccaagg cccggtccac
     2341 gtccccccgg cggccgatgt cccctttcaa aaatcggtgg cgcggagacc gaagggcgtc
     2401 gcccaaattg gaccgatcgc ccgcgtaggt cagcttgtcc agggtgacga tccggtcgtt
     2461 ccggtggtgt tccagccaat ggttgacgaa atgggagccg atgaaccccg cgccaccggt
     2521 aacgagtatt ttcaatttag tccctcacct ttatccacga cgaccttcaa a
//
LOCUS       DAOQLS010000266         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24490_length_2568_cov_3.776363, whole genome shotgun sequence.
ACCESSION   DAOQLS010000266 DAOQLS010000000
VERSION     DAOQLS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24490_length_2568_cov_3.776363"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PK362_05835"
     CDS             <1..394
                     /locus_tag="PK362_05835"
                     /inference="COORDINATES: protein motif:HMM:NF021883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNC74385.1"
                     /translation="DISHSSVERVILPDATILLDYMFHRLAAVIEGLQVYPDRMKANL
                     AKTADITCSQRLVVALTKKGLKKQEAYEIVQGHALEAWTRGTPFRPAVAADPRVAGRL
                     SRAEIEGAFDLAPFYRNIGAVLRRSLRG"
     gene            425..1243
                     /locus_tag="PK362_05840"
     CDS             425..1243
                     /locus_tag="PK362_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74386.1"
                     /translation="MTREVFVFRQVDVGESVRFAFSIVFAHAHFWFLIAAALALAGPT
                     VSGWVWGAHPPFLGRTIVFLAGGLWPLAVATRIALLRLQGKAVGVDVLMDWGAVYRLV
                     LPVALVLAVAGAGGLAFEWILSLIFRGGLPPVVAGLHAPMSLMGIMRFYPLICLGGIS
                     VAMPMSFAPWVAIDKNLGVSAAMEWASRLSKDVKMEMMFLQGMIFFTVSTVFLVGAFS
                     GGKSFPENMIAYFVYTAGFAFAAAFWTEAYRQTLAYEEPDTVKSVAPRRVFRVG"
     gene            1312..1644
                     /locus_tag="PK362_05845"
     CDS             1312..1644
                     /locus_tag="PK362_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74387.1"
                     /translation="MNVEVYIAVCLTLIVIAVAGVSVFLILALWQLRRAARALEALST
                     RLGTQVGRLEGLAGALGNVALAATGGLGRGAAVGASVVWSVIDFFRQRRRARAAEAEE
                     EPAKESVR"
     gene            1641..1919
                     /locus_tag="PK362_05850"
     CDS             1641..1919
                     /locus_tag="PK362_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNC74388.1"
                     /translation="MSDQNNNGGSLWAFLVGAGLGVAAGLLLAPRSGRESRKRLGRWI
                     EDMEDEGRDMLKEGRDLWERGKTAAQEKTDKIKRTLESVSAEFEDDRS"
     gene            1937..2500
                     /locus_tag="PK362_05855"
     CDS             1937..2500
                     /locus_tag="PK362_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC74389.1"
                     /translation="MSAPVPPRPAAWPLRWVGRVGRTIAPLFGDADFRTTLRFFRGIF
                     LFKNLARNDLARLVRKMMQKTYAPGDVVFMEGDLGRAFFVVAEGKVEVSRRNPTTGQE
                     ETVAQFGPGDFFGEMVLLDELPRSATCRVLDHTRLYVMYKDHFDILLRDSPRAAAPIL
                     HALARLLSARLRRERRAFETHNGEGPA"
BASE COUNT          382 a          809 c          846 g          531 t
ORIGIN      
        1 cgacatctcc cattcgtcgg tggagcgcgt gatattgccc gacgcgacga tcctgttgga
       61 ttacatgttc caccgcctgg cggcggtcat cgagggactt caggtctatc cggaccggat
      121 gaaagccaac ttggcgaaga cggcggacat cacctgttcc cagcggttgg tggtcgccct
      181 caccaaaaaa ggccttaaaa aacaggaagc ctacgaaatc gtccagggtc acgccctgga
      241 agcctggacc cgcgggacgc ctttccggcc ggcggtcgcc gccgatccgc gggtggccgg
      301 ccgtctgtcc cgggccgaaa tcgagggggc cttcgatttg gcgcccttct atcgaaacat
      361 cggcgcggtt ttgcgccgtt ccctccgggg ttaacctctt gatccagacc ttccggcggc
      421 gtcgatgacc cgggaagtct tcgtcttccg gcaagtcgat gtgggggaat ccgtccgctt
      481 cgctttttcc atcgtgttcg ctcacgcgca tttttggttt ttgatcgcgg ccgccctggc
      541 gctggccgga ccgacggttt cgggatgggt ttggggcgcc cacccgcctt ttctgggtcg
      601 caccatcgtt ttcctggcgg ggggcctttg gcccctggcg gtggccacgc ggatcgcgct
      661 tttgcgcctt caaggaaagg ccgtgggcgt cgacgtatta atggattggg gcgccgtcta
      721 tcgcctcgtg ctgcccgtgg cgctcgtttt ggccgtggcg ggcgcgggcg ggttggcttt
      781 cgagtggatc ctttcgttga ttttccgcgg gggattgccg cccgtggtcg ccggtcttca
      841 cgccccgatg tccctcatgg gcatcatgcg gttctatccg ctgatttgcc tgggcggcat
      901 atccgtggcc atgccgatgt ccttcgcccc ctgggtggcc atcgataaaa acctgggggt
      961 gtcggccgcc atggagtggg cgagccggct ttccaaagac gtcaaaatgg aaatgatgtt
     1021 ccttcagggg atgattttct ttaccgtgtc cacggtcttt ttggtcgggg cgttttcggg
     1081 cggaaaatct tttcccgaaa acatgatcgc ctatttcgtc tacacggccg ggttcgcctt
     1141 cgccgccgct ttttggacgg aagcctaccg ccaaaccttg gcctacgagg aacccgacac
     1201 cgtcaaatcc gtcgccccgc gccgggtctt tcgggtgggg tgacccgact tgacccggcg
     1261 gtcctttcgg cccatactta aagggaagac gcgccggtcc ggaggtcgtc catgaacgtt
     1321 gaagtctaca tcgcggtgtg tttgacgttg atcgtgatcg ccgtggcggg ggtctcggtc
     1381 tttttgattt tggccctgtg gcagctccgt cgagccgccc gggccctgga agccctgtcg
     1441 acccgattgg gcacccaggt gggccggttg gaaggtttgg ccggcgccct gggcaacgtg
     1501 gccctggcgg ccacgggcgg cctcggccgg ggcgcggcgg tgggcgccag cgtggtatgg
     1561 agcgtcatcg attttttccg gcagcgacgc cgcgcccgcg cggcggaggc cgaagaagag
     1621 ccagcgaagg agtccgtgcg atgagcgatc aaaacaacaa cgggggatcc ctctgggcct
     1681 ttttggtggg cgcggggttg ggcgtggcgg cgggtttgtt gctggcgccg cgttccgggc
     1741 gggaaagccg caagcgcctc ggtcggtgga ttgaggacat ggaagacgag ggccgggaca
     1801 tgttgaaaga gggccgggac ctctgggagc ggggcaagac cgccgcccag gagaaaaccg
     1861 acaaaattaa acgcaccctg gaatcggtgt ccgccgaatt cgaggacgac cggtcctgag
     1921 cgccgtggac gacgtcgtgt ccgcccccgt tcctccccgg cccgccgcct ggcccctccg
     1981 ctgggtgggc cgcgtcggac ggaccatcgc gccgctcttc ggcgacgcgg atttccgaac
     2041 gaccctccgt tttttccgcg gcatctttct ttttaaaaac ctcgcccgaa acgacctggc
     2101 ccgcctggtg cgtaaaatga tgcagaagac ctacgccccc ggggacgtcg tgttcatgga
     2161 aggggacctg ggccgcgcct tcttcgttgt ggccgagggc aaggtggagg tgtcccgccg
     2221 caacccgacc accggccagg aggaaaccgt tgcccaattc gggccggggg acttcttcgg
     2281 cgagatggtc cttttggacg agttgccccg ttcggcgacc tgccgggtgc tcgaccacac
     2341 gcgtctctac gtgatgtaca aagaccactt cgatatcctg ttgcgggatt ctccccgggc
     2401 cgccgcgccg atcctgcacg ccctggcgcg tcttttgagc gcccgcctcc gccgggaacg
     2461 gcgggccttc gaaacccaca acggcgaagg tcccgcctag tttctcccgt ggccgacccc
     2521 ctccccaccc gttcgccacg gtcaccagca tcgacaaggc cgcctggg
//
LOCUS       DAOQLS010000267         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24683_length_2558_cov_2.850979, whole genome shotgun sequence.
ACCESSION   DAOQLS010000267 DAOQLS010000000
VERSION     DAOQLS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24683_length_2558_cov_2.850979"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PK362_05860"
     CDS             <1..894
                     /locus_tag="PK362_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC74390.1"
                     /translation="KTTTLYAILNETLSPYKNYETIEDPVEYRLEGANQVQVRENLGL
                     SFAATLRCTLRQDPDVILLGEIRDEETADVAFKAALTGHLVLSTLHTNSTVASLTRLI
                     DIGVKPYLLASALEGIIAQRLVRRICESCRTRVEPAAETLALLRLRPEDFPTAFAGTG
                     CERCDHSGYRGRTGLFELMVVTDELRRGIAGGVEESAVTGLARAAGMRTLLEDGLEKA
                     RAGVTTLEEVLRVMGPPTRYEEPCGACRRPFEVTHGFCPFCGSERRNRCGGCHRTLEP
                     LWSHCPGCGQKRSLPTARSAL"
     gene            complement(896..1636)
                     /locus_tag="PK362_05865"
     CDS             complement(896..1636)
                     /locus_tag="PK362_05865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC74391.1"
                     /translation="MKDRTAIAPVGRWWRGISWPQWLSPRRWRLGRWAGGRRARRKER
                     EERLFHAVRRLRRRNRRLRTLVALDGLTGVANRRALNERLRAEWFRARRGSYPLALLL
                     VDIDYFKAFNDRFGHLAGDDALKNVARALMETVRRPGDLVARYGGEEFAVLLPHTDVP
                     GAALVAESLRRAVNHFSRRSAPEGASLTVSVGVASWIPDGRAAPLDLLAATDQALYRA
                     KALGRNRVELDWPSWGRPLRGGVAPAVA"
     gene            complement(1633..2289)
                     /locus_tag="PK362_05870"
     CDS             complement(1633..2289)
                     /locus_tag="PK362_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008042842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNC74392.1"
                     /translation="MTGPGRGLLILLIAGGAHAAEPARPVASALENVWRALADGNHRF
                     ARGALRSRPTIERRRELVAGQHPRAMILSCSDSRTPPELLFDQGLGDLFVVRSAGEVV
                     DPVALGSLEYAADHLGVGVLVVMGHRKCGAVAAALSGEPMPTPNLDAIVKRIRPALPD
                     PKMKETDRVRLAEESNVREAVQNILLESPLLRERVARGRLLIVPALYDLSSGRVEKLP
                     "
     gene            complement(2286..>2558)
                     /locus_tag="PK362_05875"
     CDS             complement(2286..>2558)
                     /locus_tag="PK362_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC74393.1"
                     /translation="DLLAARAEPDVLSCLEKEEVVRRLREGVDALPEKERHVVSFYYF
                     EELTLREIGEVLGVSESRVCQIHARAVKIIERWLASPRATPAGCRP"
BASE COUNT          452 a          895 c          833 g          378 t
ORIGIN      
        1 aagacgacca cgctctacgc catattgaac gagaccctga gcccgtacaa gaattacgaa
       61 accatcgaag atcccgtgga ataccgtctg gagggggcca accaggtcca ggtccgggaa
      121 aacctggggc tgtccttcgc cgccactctt cgctgcaccc tgcggcagga ccccgacgtc
      181 atcctcctgg gggaaatccg cgacgaggaa accgcggacg tggccttcaa ggcggccctc
      241 acgggccacc tcgtcctctc caccctgcac accaacagca ccgtggcgtc cctcacgcgg
      301 ctgatcgaca tcggggtcaa accgtacttg ctcgcctcgg ccctggaagg catcatcgcc
      361 cagcggttgg tccggcgaat ttgcgaaagt tgccgcaccc gggtggaacc ggcggccgaa
      421 accttggccc tccttcgcct gcggcccgag gatttcccga cggccttcgc cgggaccggc
      481 tgcgaacggt gcgaccattc cggctaccgg ggacgcacgg gcctcttcga attgatggtc
      541 gtcacggacg aacttcgacg gggaatcgcc gggggggtgg aggaatcggc ggtgacgggg
      601 cttgctcggg cggccggcat gcgcaccctg ttggaagacg ggctggaaaa agcccgggcg
      661 ggggtcacca ctctggagga ggtcctgcgg gtgatggggc cgccgacccg ttacgaggaa
      721 ccttgcgggg cctgtcgccg ccccttcgag gtgacccacg ggttttgccc tttttgcgga
      781 tcggaacggc gaaaccgctg cggggggtgc caccgcaccc tggagccgct ctggagccac
      841 tgccccgggt gcggccagaa gcgatcgttg ccgacggcgc ggtcggccct ttaaatcacg
      901 ccacggccgg ggccacgccg ccccggagag gacgacccca ggagggccag tccagttcga
      961 cccggtttcg gcccagggcc ttggcccggt agagggcctg gtccgtggcg gccaacaaat
     1021 ccagaggcgc ggcccggccg tcggggatcc aggaggcgac gccgacgctg accgtgaggg
     1081 aagccccttc cggcgcggac cggcgggaga aatggttcac ggcccgccgg agactttcgg
     1141 ccaccagggc cgcgccgggc acgtcggtgt ggggaaggag aacggcgaat tcctcccccc
     1201 cgtaacgggc caccaaatcg cccggacggc ggaccgtctc catgagagcg cgggcgacgt
     1261 tttttaacgc gtcgtccccc gccaaatggc cgaaccggtc gttgaacgcc ttgaaatagt
     1321 cgatgtcgac caacaggagc gccaacggat aggatccccg ccgtgcccga aaccattcgg
     1381 cccggagcct ttcgttgagg gcccgccggt tggccacgcc cgtgagtccg tccagagcca
     1441 ccagggttcg caatcggcga ttgcgacggc gaagccgccg gaccgcgtga aacaaccgct
     1501 cttcccgctc ctttcgccgg gcgcggcgcc cgcccgccca gcgcccgagg cgccatcggc
     1561 gcggggacaa ccattggggc caggaaatcc cccgccacca ccggccgacg ggagcgatcg
     1621 ccgtccgatc cttcacggga gtttctccac ccgaccggac gacaaatcgt agagcgcggg
     1681 aacgatgagc aatcgccccc gagccacgcg ttcccgcagc agcggacttt ccaacaggat
     1741 gttttgcacc gcctctcgga cgttggactc ctcggccagc cgaacccggt cggtttcctt
     1801 cattttcgga tcgggcaggg ccggccggat tcgtttgacg atcgcgtcca ggttcggggt
     1861 gggcatgggt tcccccgaga gggcggcggc caccgctccg cacttccgat gccccatcac
     1921 caccaggaca cccaccccca gatgatccgc ggcgtattcc aaactgccca aggccacggg
     1981 gtccacgact tccccggcgc tccggaccac gaacaaatcc cccaacccct gatcaaacaa
     2041 caattcgggc ggggttcggc tgtcggaaca ggacaaaatc atggcccggg ggtgttgccc
     2101 cgcgaccaat tcccgtcgac gctcgatcgt cggacgggaa cggagggcgc cccgggcgaa
     2161 acggtgattg ccgtccgcca gggcgcgcca cacgttttca agggccgacg cgaccgggcg
     2221 cgccggttcg gcggcgtggg ctccgccggc gatcaaaagg atcaagagtc cccgcccggg
     2281 acccgtcacg gccggcatcc cgcgggggtg gcccggggcg aggccaacca acgctcaatg
     2341 attttgacgg cccgggcgtg gatctgacag acccgcgatt ccgacacgcc gaggacttcc
     2401 ccgatttccc gaagagtgag ctcctcgaaa tagtagaagg acacgacgtg ccgttccttt
     2461 tcgggcaacg cgtccacccc ctcccggagg cgacgaacca cttcctcttt ttcgagacag
     2521 gacagcacgt ccggttccgc ccgggcggcc aggaggtc
//
LOCUS       DAOQLS010000268         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24730_length_2555_cov_3.390400, whole genome shotgun sequence.
ACCESSION   DAOQLS010000268 DAOQLS010000000
VERSION     DAOQLS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24730_length_2555_cov_3.390400"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PK362_05880"
     CDS             <1..616
                     /locus_tag="PK362_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74394.1"
                     /translation="SDALYQSAPGAGGDYREARKQKARQSAQYNLKSEFDESQGINED
                     AFAQQVEREINFFSNVNRAPATGEAAGTPLRIKVPNAGQVYRFSKVLVQENEPMTLGA
                     LHLHGALIPLIKLLLGLWILWTLYRSRLRLRQAGEGLVRLAERHPRELGWIRSPLGPA
                     LGLFVLGLGLSWVSRFLSLVAFLGAFALGGRWIVGRWLSGRKNP"
     gene            613..852
                     /locus_tag="PK362_05885"
     CDS             613..852
                     /locus_tag="PK362_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74395.1"
                     /translation="MNSFLERAFGRPAAEWSRLHKWAVRLGAGAAGFAAVFALHVLTL
                     ILVGVYRAPGFGGVWFCGFLFAAVLVFLADRRLKS"
     gene            complement(854..1765)
                     /locus_tag="PK362_05890"
     CDS             complement(854..1765)
                     /locus_tag="PK362_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNC74396.1"
                     /translation="MPKTPPPVHGHPMVKAGRLMSLTFRHWREDHCSLLAAGLAYYTL
                     ISLVPLSVIVLAVGGRALGNAASLGHLVPALSGALGPELAGSISRIVTEASFKGLVHA
                     TTLSVGLLIWAASIVFAHLQRALNIVWDVKPRPGVAGALLNRLMSFAMVLAVGFLFLG
                     FAALNAGMGLVRNFLAKLAPLLMERLHLWDTTNLVLFFVLQAVLWAVVFKFLPALKLA
                     WRDVRVGAAVTALLTTLGVYLLKIYFREIPELSVFGAAASVMAVLIWVYFSTQIFLFG
                     AEFTWAWAHLFGSRAGAPPPVEYPRSI"
     gene            complement(1766..>2555)
                     /gene="arsS"
                     /locus_tag="PK362_05895"
     CDS             complement(1766..>2555)
                     /gene="arsS"
                     /locus_tag="PK362_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570255.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNC74397.1"
                     /translation="DHCLRVLRDFDIPTLDITGGAPELNPHFRELVGGARALERRVID
                     RCNLTVLEIPAQADLAGFLAAHRVEVVASLPYFEADRTDAQRGDGVFDRSLRGLRRLN
                     ALGYGRPDSGLILNLVYNPAGAFLPPDQGDLEVQFRRELRDRWGVEFNRLFALANMPI
                     GRFLEFLRATGNYEGYMTRLANAFNPAAVPGLMCRNLLSVGWDGALYDCDFNQMLDWP
                     LVEGSPAHIRDFDPDRLARRAVAVGNHCFGCTAGGGSSCGGSLT"
BASE COUNT          504 a          825 c          811 g          415 t
ORIGIN      
        1 ctccgacgcc ctgtaccaat ccgccccggg cgccggcgga gactaccggg aagcccgcaa
       61 gcaaaaggcc cgccagtccg cccaatacaa cctcaaaagc gaatttgacg aatcccaggg
      121 catcaacgag gacgccttcg cccagcaggt ggaacgggag atcaatttct tcagcaacgt
      181 caaccgcgcg cccgcgacgg gtgaagccgc gggaacgccc cttcgaatta aggtacccaa
      241 cgccgggcag gtctaccggt tttccaaggt tctggttcag gagaacgagc cgatgacgct
      301 gggcgccctg catcttcacg gggcgctgat cccgttgatc aagcttctgc tgggcctttg
      361 gatcctttgg acgctttatc ggtcccgtct tcgcctgcgg caggccggcg agggcctcgt
      421 ccgattggcc gagcgccatc cccgggaatt gggctggatt cggtcgcccc tcggaccggc
      481 tttgggattg ttcgttctgg gactggggtt gtcctgggtg tcgcgctttt tgtccctggt
      541 cgcctttttg ggggccttcg ccctgggggg ccggtggatc gtcggccgtt ggctgtccgg
      601 aaggaaaaac ccgtgaattc ctttctggaa agggccttcg gccggccggc cgccgagtgg
      661 agccggctcc acaaatgggc cgtccgtttg ggcgcggggg ccgcggggtt cgccgcggtg
      721 ttcgccttgc acgttttgac gctgatcttg gtgggggtct atcgggcgcc ggggttcggc
      781 ggcgtttggt tttgcgggtt tcttttcgcc gcggtcctcg tgttcctcgc ggaccgtcgt
      841 ctgaagtcct gattcaaatc gatcgggggt attccaccgg cggaggcgcg ccggcccggg
      901 atccgaacaa atgggcccag gcccaggtaa attcagcccc gaataaaaaa atctgcgtcg
      961 agaaataaac ccagatcagc acggccatga ccgaagcggc cgccccgaag accgacaatt
     1021 ccggaatttc ccgaaaatag atttttaaca ggtacacccc cagcgtcgtg agaagcgccg
     1081 tcaccgccgc gcccacccga acgtcccgcc aggccaattt cagggcggga agaaacttga
     1141 acaccaccgc ccacagaacg gcctggagca cgaaaaacag caccaggttc gtcgtgtccc
     1201 acaggtgaag acgctccatc agaagcggcg ccagtttggc cagaaaattc cgaaccagcc
     1261 ccatccccgc gttcaacgcc gcgaatccca gaaacaaaaa tcccacggcc aacaccatgg
     1321 cgaaactcat gagccggttg agcagggccc cggccacccc cggccggggt ttcacgtccc
     1381 acacgatgtt gagggcccgc tgcaggtggg cgaaaacgat ggaggcggcc caaatcaata
     1441 gcccgacgct caaggtcgtg gcgtggacca gacctttgaa ggacgcttcc gtgacgatgc
     1501 gggaaatgga cccggccagc tccggaccca gggcccccga cagggccggg accaggtggc
     1561 cgagggaggc ggcgttgccc agagcgcgcc cgccgacggc cagcacgatg accgacaggg
     1621 gcaccaggga gatgagggtg taataggcca accccgccgc caggagcgaa caatggtcct
     1681 cccgccagtg gcgaaaggtc agggacatca agcgtccggc cttgaccatc ggatggccgt
     1741 gaacgggcgg cggcgtcttc ggcatttacg tcaggcttcc gccgcaggac gacccgccgc
     1801 ccgcggtgca cccgaaacaa tggttgccga ccgcgacggc ccgccgggcc aggcggtcgg
     1861 gatcgaaatc ccgaatgtgg gccggcgatc cctcgaccag gggccaatcc agcatttgat
     1921 tgaaatcgca atcgtacaaa gcgccgtccc atcccaccga caggaggttc cggcacatga
     1981 ggccggggac cgccgccggg ttaaaggcgt tggccaggcg cgtcatgtat ccttcgtaat
     2041 tgcccgtggc ccgaagaaat tccagaaacc gtccgatggg catgttggcc agggcgaaga
     2101 ggcggttgaa ttccaccccc caccggtccc ggagttcccg ccggaattga acttccaggt
     2161 ccccttggtc gggcggaaga aaggccccgg cgggattgta aaccaaattc aaaatcaagc
     2221 cgctgtccgg gcggccgtat ccgagggcgt tcaatcgccg caacccccgc agggaacgat
     2281 cgaacacgcc gtcgccccgc tgggcgtcgg tgcggtccgc ctcaaaatag ggaagggagg
     2341 ccaccacctc cacccggtgg gcggccagga acccggccag gtccgcctgg gcgggaattt
     2401 ccaataccgt caaattacag cggtcgatga cgcggcgttc cagggcccgg gcccccccga
     2461 ccaactcccg aaaatggggg ttcaattccg gcgcgccgcc ggtgatgtcc agggtgggaa
     2521 tatcgaaatc ccgaaggacc cgcaggcagt gatcc
//
LOCUS       DAOQLS010000269         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24735_length_2555_cov_3.114800, whole genome shotgun sequence.
ACCESSION   DAOQLS010000269 DAOQLS010000000
VERSION     DAOQLS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24735_length_2555_cov_3.114800"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PK362_05900"
     CDS             complement(<1..931)
                     /locus_tag="PK362_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC74398.1"
                     /translation="MTEPFNPASIEPKWQKRWEDAGVFKSDPDPRKPKLYILDMFPYP
                     SGAGLHVGHPEGYTATDILARMKRMQGFNVLHPMGWDAFGLPAENHAIQTGQAPRVVT
                     QNNINNFRRQIKSLGFSYDWSREVDTTDPAYYKWTQWIFLQLYKKGLAFEGVSPINFC
                     PSCRTGLANEEVHQGACERCGTPVERRDMRQWLLKITAYADRLLEDLEGLDWPESTLA
                     MQRNWIGRSEGAEVIFQGADGAAGKSVTVFTTRPDTLFGATYLVLAPEHPLVPVFATA
                     DRRSDVEAYQKAARNKSDLERTDPVAAVSRLESV"
     gene            complement(928..1680)
                     /locus_tag="PK362_05905"
     CDS             complement(928..1680)
                     /locus_tag="PK362_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNC74399.1"
                     /translation="MEKVRLDHLLVRRGLAETGAKAQALILAGKVRAPGLKNVKPGEK
                     VRLDQEIELVADLPYVSRGGEKLAAALDEFGVTVAGRFCLDVGASTGGFTDCLLQRGA
                     VGVLAVDVGHGQLHWKLRNDPRVENLEKTHARDLTADRVAASAGPALWPGLLTVVDVS
                     FISLDKVLPAVAGATPPGGEFVALLKPQFEVGPKDVPGGIVRDDAVREAVLSRVQSQA
                     DGWGFRFEKSMVSPLKGRDGNVEYLLHLRKKT"
     gene            complement(1685..>2555)
                     /locus_tag="PK362_05910"
     CDS             complement(1685..>2555)
                     /locus_tag="PK362_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNC74400.1"
                     /translation="AITAAMPEGTGLDLFRKEIPGRFYDVGLGEQHAVTFAAGMACGG
                     LKPVVAIYSTFLQRGFDQMFHDVALQKLPVVFCLDRAGLVGEDGPTHHGAYDLSYSRM
                     FPNMTVMAPKDENELQHMLKTALNLPGPSVIRYPRGAGQGVALDTAYHLIPLGKGEIL
                     REGEDVTLLAIGHPVVAAMEAAEKLAAEGVSVRVVNARFAKPLDREFLLGVARRTPLL
                     VTLEENTFVGGFGDAVREALEGENIAVRTIALPDAFVEHGAQPKIREKAGLSAEKIAA
                     RVMEFRRQRQAVS"
BASE COUNT          422 a          848 c          813 g          472 t
ORIGIN      
        1 acaccgactc caggcgcgac accgccgcca ccgggtcggt ccgctccagg tccgacttgt
       61 tccgcgccgc tttttggtag gcctccacgt cgcttcgccg atccgccgtg gcgaagacgg
      121 gcaccagcgg gtgttcgggc gccagcacca aataggtcgc gccgaagagc gtatcgggcc
      181 gcgtggtgaa gaccgtgacg gatttccccg ccgcgccgtc cgccccctgg aaaatcacct
      241 cggccccctc ggagcggccg atccaattgc gttgcatggc cagggtggat tcgggccagt
      301 ccaggccctc cagatcctcc aacaagcggt cggcgtaggc cgtgattttc aacagccatt
      361 ggcgcatgtc ccggcgttcc acgggggttc cgcagcgctc gcaggcgccc tggtggactt
      421 cctcgttggc caggcccgtt cggcagctgg ggcagaaatt aatcgggctg acgccctcga
      481 aggccaggcc tttcttgtag agctgaagga aaatccattg ggtccatttg tagtaagccg
      541 ggtccgtggt gtccacctcc cgggaccagt cgtaggagaa ccccagggat ttgatctgcc
      601 tccggaaatt gttgatgttg ttttgggtga cgacccgggg ggcctggccg gtttgaatgg
      661 cgtggttttc cgccggcagg ccgaaggcgt cccaacccat ggggtgcagg acattgaaac
      721 cctgcatgcg tttcatgcgg gccaggatgt cggtggcggt gtagccctcc gggtgcccca
      781 cgtggaggcc cgcgcccgag gggtagggga acatgtcgag aatgtagagt ttcggcttcc
      841 gggggtccgg atccgattta aaaacgccgg cgtcctccca acgtttttgc catttgggtt
      901 cgatggaagc ggggttgaag ggttcggtca tgtttttttg cgcaggtgaa gcaaatattc
      961 gacgttgccg tcccggccct tgagcgggga gaccatggat ttttcgaatc gaaatcccca
     1021 gccgtccgcc tgggattgaa cgcgggacaa gaccgcctcc cgcaccgcgt cgtcccgcac
     1081 gatgcccccc ggaacgtcct tggggcccac ttcaaattgg ggcttcaaca gcgccacaaa
     1141 ctctccgccc ggcggcgtcg ccccggccac cgccggcagg actttgtcca gggagatgaa
     1201 agacacgtcc accaccgtga gcaacccggg ccagagcgcc gggcccgcgc tcgccgccac
     1261 ccgatcggcg gtcaagtccc gggcgtgggt tttttccaaa ttttcaacgc gcgggtcgtt
     1321 ccgcaatttc cagtggagtt ggccgtggcc cacgtccacc gccagaaccc cgacggcgcc
     1381 ccgttgcaag agacagtcgg tgaacccgcc cgtggaggcg cccacgtcca aacaaaaccg
     1441 ccccgcgacc gtcacgccga actcgtccag ggccgcggcc aatttttccc cgccccggga
     1501 aacgtagggc aggtcggcca ccagctcgat ttcctggtcg agccggactt tttcgccggg
     1561 cttcacgttc ttcaaccccg gcgcccgcac cttgccggcc agaatcaaag cctgggcctt
     1621 ggcgccggtt tcggccaatc cccgccgaac cagcaggtga tccagtcgga ctttttcgat
     1681 cagatcagga gaccgcttgc cgttgccggc gaaattccat cactcgggcc gcgatcttct
     1741 cggcggacaa gcccgccttt tcgcgaattt tgggctgggc cccgtgttcc acaaaggcgt
     1801 cgggcagggc gatggttcgg accgcgatgt tttccccctc cagagcctcc cggacggcgt
     1861 cgccgaagcc gcccacgaaa gtgttctcct ccagggtcac gaggagcggc gttcgccgcg
     1921 ccacgcccag caaaaattct cggtccaggg gcttggcgaa ccgcgcgttc accacccgga
     1981 cggagacgcc ttcggcggcc aatttctcgg cggcttccat ggcggccacc accgggtggc
     2041 cgatggccag gagcgtcacg tcctcgcctt cccgcaggat ttcgcccttc cccaggggaa
     2101 tcagatggta agccgtgtcc agggccacgc cttgtcccgc gccccggggg tagcggatca
     2161 ccgacgggcc gggcaggttg agggccgttt tcagcatgtg ctgcagttcg ttctcgtcct
     2221 tgggcgccat gaccgtcatg ttcgggaaca tgcgggagta ggacaggtcg taggccccgt
     2281 ggtgggtggg gccgtcctcg cccaccaggc ccgcccggtc caggcagaac acgacgggca
     2341 atttttggag cgccacgtcg tggaacattt ggtcgaaccc ccgttgcagg aaggtcgagt
     2401 aaatggccac cacgggtttc aaccccccgc aggccatgcc ggcggcgaag gtcaccgcgt
     2461 gctgttcgcc caggccgacg tcgtaaaacc tcccggggat ctcttttcgg aaaaggtcca
     2521 gcccggtgcc ctcgggcatg gcggcggtga tggcg
//
LOCUS       DAOQLS010000270         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_24810_length_2551_cov_3.530048, whole genome shotgun sequence.
ACCESSION   DAOQLS010000270 DAOQLS010000000
VERSION     DAOQLS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24810_length_2551_cov_3.530048"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="PK362_05915"
     CDS             complement(<1..1665)
                     /locus_tag="PK362_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74401.1"
                     /translation="MTFSKAQLKKLKSTIAGATASVFAFTTVSVPVVEANFWQDRQKA
                     ARTVADPSSADAGTAESRSLYAGMFKGANPMLLDEKAVDGVAAGARDLVTKGLADLVT
                     PRTTDQKPLPAWMRTLVTPFGEIDDFHVTGSLRNAADWSFDKSADPVIVLIQDVHNVP
                     EAQLNISKIMESYQTRAGLSVVGLEGATGGLLTDKFRAMNAPEALRWASDFLINERVL
                     SGAEYFGLNAPRSATLWGIENEGAYLANVEAYKQTVALRPDVAAFEASANGALAALQE
                     KVYSADLKTFERQRSLYADEKISLSDYVESIAAGPALRAGVNPNAYPNIALFRKAILL
                     EKTIDFKKVEAERTEFLQALTARLSKDDLTRLVALSMEFRLHHVGFGDYYKALSAAAA
                     RVKVDLSNTPELKKYVSYALLTDGIARTSFLNEIGRLEDSVTEGLATSTAQKEAARLS
                     RDYVLLHKLLHHNLTFHDWSAFLKRVAELRAFDARLAAAGQKNGVPVAKAEFGFPLAP
                     YEAFYTSAQARNEHFVQNLFDRLRASGARSAVLVAGGFHTEGLRDLL"
     gene            1842..>2551
                     /locus_tag="PK362_05920"
     CDS             1842..>2551
                     /locus_tag="PK362_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNC74402.1"
                     /translation="MPSTNSFGTKVHLTPGGPHYFRLPTLEEKGVANVSRLPYSIRIL
                     LENALRNEDGRLVTRKDVETLLHWVPKNLPEKEIPFIAARVLLQDFTGVPVVVDLAAM
                     RDAARAVGVDPKKINPLFPVDLVIDHSVQVDAFGSAAAFTANVETEYARNRERYVFLK
                     WGQKALRNFRAIPPDMGIVHQVNLEYLAPVVIERDGVLFFDTVIGTDSHTTMINGLGV
                     LGWGVGGIEAEAAMLGQP"
BASE COUNT          490 a          761 c          808 g          492 t
ORIGIN      
        1 cagcaggtcc cgaaggccct cggtgtggaa accgccggcc accaggacgg cggaccgggc
       61 gccggaggcg cggaggcggt caaaaaggtt ttggacgaaa tgttcgttgc gggcctgggc
      121 gctcgtgtaa aaggcttcgt acggggccag cggaaaaccg aattcggcct tcgccacggg
      181 gacgccgttt ttctgtccgg cggcggcgag gcgcgcgtcg aaggcgcgga gctcggcgac
      241 gcgcttcagg aaggcgctcc aatcgtggaa cgtcaaattg tggtggagca gtttgtgaag
      301 aaggacgtag tcccgggaaa gacgggccgc ctccttctgc gccgtggagg tggcgagacc
      361 ttcggtcacg gaatcctcga gacggccgat ttcgttcaga aaagaggtcc gggcaatgcc
      421 gtcggtcaac agcgcgtagg aaacgtattt cttgagctcc ggcgtgttcg aaaggtccac
      481 cttcacgcgc gcggcggcgg cgctcagggc cttgtagtag tcgccgaaac ccacgtggtg
      541 cagacgaaat tccatgctga gggccaccag gcgcgtcaga tcgtctttcg agagacgagc
      601 cgtcagagcc tgaaggaatt ccgttcgttc cgcctccact tttttaaagt cgatggtttt
      661 ttcgagaaga atggccttgc ggaaaagcgc gatgttcgga taggcgttcg ggttcacgcc
      721 ggcgcggagg gccggcccgg cggcgatgga ttccacgtaa tcgctcaggg aaatcttctc
      781 gtcggcgtag agggaccgct gacgttcaaa ggttttcaag tccgcgctgt aaactttctc
      841 ctgaagggcg gccaaggcgc cgttggcgga ggcttcgaag gcggccacgt cgggccggag
      901 cgccaccgtt tgcttgtagg cctccacgtt ggccaggtag gccccttcgt tttcaatgcc
      961 ccacagcgtt gccgaacggg gcgcgttcag accgaagtat tcggccccgc tcaacacccg
     1021 ctcgttgatc aaaaagtcgg aggcccagcg gagggcttcc ggggcgttca tggcgcggaa
     1081 cttgtcggtc aacaggccgc cggtcgcccc ttccaaaccg acgacggaaa gacccgcccg
     1141 ggtttggtac gactccatga ttttcgagat gttgagttga gcttcgggaa cgttgtggac
     1201 gtcttgaatc aggacgatca cggggtcggc ggatttatcg aaagaccagt cggcggcgtt
     1261 ccgaagggaa cccgtcacgt ggaaatcgtc gatttcgccg aagggcgtga ccagggtccg
     1321 catccaagcg ggcaggggtt tttgatcggt ggtgcggggc gtcaccaggt cggcgaggcc
     1381 cttggtcacc aggtcccggg cgccggcggc aacgccgtcg acggctttct cgtccaacag
     1441 catggggttg gcgcccttga acatcccggc gtacagggaa cgggattcgg ccgttccggc
     1501 gtcggcggag gaggggtccg cgacggtacg ggcggctttc tgacggtcct gccaaaaatt
     1561 ggcttccacc acgggcacgg acaccgtcgt aaaggcaaac accgacgcgg tggcgccggc
     1621 gatcgtggat ttcaactttt tgagctgggc tttggaaaac gtcatttgtt cccctccggg
     1681 aaaaatcttc gaattgccct cttattatgt tcgcgccggt caaatatccc cttaagtaaa
     1741 tgtaaattag atgtaacact taaaatctcc cccatcgcac gaggaaacgg ccaacgcccg
     1801 gcgattttgt aaaatccgga aacttcccaa acggagcgac catgccttcc accaattctt
     1861 tcggcaccaa agttcacttg acccccggcg gtccccacta tttccgtcta ccgaccctgg
     1921 aggagaaggg agtggccaac gtgtcgcgcc ttccctattc catccgaatt cttctggaaa
     1981 acgcccttcg caacgaggac ggccggctgg tgacccggaa ggacgtggag acccttctcc
     2041 actgggtgcc caaaaatctt cccgaaaagg aaatcccctt catcgccgcc cgggtcctcc
     2101 tgcaggattt caccggggtt cccgtggtcg tcgacctggc cgccatgcgg gacgccgccc
     2161 gggccgtggg cgtggacccc aaaaagatca atccgctctt tcccgtcgat ctggtgatcg
     2221 accactccgt ccaagtggac gccttcggct ccgccgcggc gttcaccgcc aacgtcgaaa
     2281 cagaatacgc ccgcaaccgc gagcgctacg tgtttttgaa atgggggcag aaggcccttc
     2341 ggaatttccg cgccatcccc ccggacatgg gcatcgtcca ccaggtgaac ctggaatacc
     2401 tggcccccgt cgtgatcgag cgggacggcg tgctgttttt cgacaccgtg atcggcaccg
     2461 actcccacac gaccatgatc aacgggttgg gcgtcctggg ctggggcgtg ggcggcatcg
     2521 aggccgaggc ggccatgctg ggccaaccga t
//
LOCUS       DAOQLS010000271         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25117_length_2533_cov_4.100081, whole genome shotgun sequence.
ACCESSION   DAOQLS010000271 DAOQLS010000000
VERSION     DAOQLS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25117_length_2533_cov_4.100081"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK362_05925"
     CDS             <1..327
                     /locus_tag="PK362_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74403.1"
                     /translation="LRLQRERQQGDYFPTVREYTELFGLNTDRLRKAKPDCLVLHPGP
                     MNRGVEISSEVADGPRSMILEQVTNGIAVRMAVLYVFANPEGLPATPPDGKQAAKAVT
                     ALKALE"
     gene            324..1643
                     /locus_tag="PK362_05930"
     CDS             324..1643
                     /locus_tag="PK362_05930"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNC74404.1"
                     /translation="MTSPKSPETLLIRGGRLIDPENRRDGEFDLLVAEGKVARVGKSL
                     AAPAGAQVIDAKGLIVAPGLVDIHVHLREPGNEGAETIESGTRAAAAGGVTSVVAMPN
                     TQPPIDNVSGVRFLLRRAAETATVRVWPTGAITVGRSGERLAEIGAMAEAGAVAVTDD
                     GNAVPETQLLRRALDYAKMFDLTVIEHAEDKSLMADGVMNEGPLATRLGHKGIPRQAE
                     SIAVARDIALAELTGAHLHVTHISTRESVDLIRAAKRRGVRVTSDATPHHFTLTEDAI
                     LRYGPNAKMNPPLRTEADRKAIIEGLVDGTIDAIASDHAPHTRASKEQEFSAAPFGII
                     GLETLLPLTVTQLIEPGHLSWPDAIRRLCSGPAKVLSLPVGDLGEGQPADLVVIDPTE
                     ERTVSGFSSKSQNSPFLGWTLRGFPQLVLVEGRVVLRREALRAAPAR"
     gene            1706..>2533
                     /locus_tag="PK362_05935"
     CDS             1706..>2533
                     /locus_tag="PK362_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNC74405.1"
                     /translation="MIVLTLLYFVYRAFPDALRNYDGLMFSSVVSQESGMWRSEMWSY
                     SSHFLFNPVGWLFHRGAVTLGLHQRGFITLFQMNALLSCGTVAVLGHVFSEAIGDRWS
                     ALPLALGAAHSANFWSRASECSVYALHLFAAALVFWALWRDARRPSVSTLAALGAAVA
                     FAVLTHISAVFWVPGVFLAVGIQNGRWRHAVVLGAASAAAVFLVFAATYRIVDLESLA
                     AWYRNQGSVGPLAAGTPDGLPGWRWAPDAPFLFRGLVGVLWAVPAGGGLLPWAAGAVG
                     "
BASE COUNT          371 a          874 c          836 g          452 t
ORIGIN      
        1 ctgcggctcc agcgggagcg gcaacagggc gattattttc cgacggtgcg cgaatacacg
       61 gaactcttcg gcctcaacac cgaccgtctg cggaaggcca agccggattg cctggtgctc
      121 caccccggcc ccatgaaccg cggcgtggaa atttcctcgg aagtggccga cggcccccgg
      181 tccatgattt tggagcaggt caccaacggc atcgccgtgc gcatggccgt tctctacgtg
      241 ttcgccaatc ccgagggatt gcccgcgacg cccccggacg gcaagcaggc cgccaaggcc
      301 gtgaccgccc tgaaggccct cgaatgacgt cccccaaatc ccccgaaacc ctgttgatcc
      361 gcggcggccg actgatcgac cccgaaaacc gccgggacgg cgaattcgac cttttggtcg
      421 ccgagggcaa agtggcccgg gtcggcaaat ccctggcggc cccggcgggc gcccaggtga
      481 tcgacgccaa aggcctcatc gtcgctccgg ggctcgtgga catccacgtc catcttcggg
      541 agcccggcaa cgaaggggcg gaaaccatcg aatccggaac ccgggcggcc gcggccggcg
      601 gcgtgaccag cgtggtggcc atgcccaaca cccagccccc catcgacaac gtgtcgggcg
      661 tccgattttt gcttcgccgc gcggccgaaa cggcgacggt gcgggtgtgg cccaccgggg
      721 ccatcaccgt cggccgttcg ggcgaacgat tggccgagat cggcgccatg gccgaggccg
      781 gggcggtggc cgtcaccgac gacggaaacg ccgtgccgga aacccagctc ctccgccggg
      841 ccctggacta cgcgaagatg ttcgatctga ccgtgatcga gcacgccgag gacaagtcgc
      901 tgatggcgga cggcgtgatg aacgaaggcc ccctggccac gcgcctcggg cacaagggca
      961 ttccccgaca ggccgaatcc atcgccgtgg cccgggacat cgccttggcg gaactcacgg
     1021 gcgcccatct gcacgtaacc cacatctcca cccgggaatc ggtggacctg atccgcgccg
     1081 ccaaacgtcg tggcgttcgg gtgacgtccg acgccacgcc gcaccatttt accctcacgg
     1141 aggacgccat cctccgctac ggccccaacg ccaaaatgaa tccccccctg cgcaccgaag
     1201 ccgaccggaa agccatcatc gaggggttgg tggacgggac catcgacgcc atcgcctcgg
     1261 accacgcgcc ccacacccgc gcctccaagg agcaggaatt ttccgccgcc cccttcggca
     1321 tcatcgggtt ggaaacgctg ttgcccctca cggtcaccca gttgatcgaa cccggccatc
     1381 tgagctggcc cgacgcgatt cgccgtcttt gcagcggccc ggcgaaagtt ttgagcctgc
     1441 cggtgggcga cctgggcgaa gggcagcccg ccgacctggt ggtcatcgac cccacggagg
     1501 agcgcacggt gtccggcttc agttccaaga gccagaattc cccgtttctg ggctggaccc
     1561 tgcgggggtt cccgcagttg gtcctggtgg agggccgcgt ggttctccgt cgcgaagccc
     1621 tgcgggccgc gccggcccga tagcgtcctt tcccttggcg aacaacgccg gccccggccg
     1681 gtcgtgggcg gggggggctt tggccgtgat cgtcctgacg ctcctttatt tcgtctaccg
     1741 cgcctttccc gatgcgctac gcaattacga cggcctgatg ttttccagcg tcgtgtcgca
     1801 agagagcggg atgtggcggt cggaaatgtg gtcctactcc tctcattttc tgttcaatcc
     1861 cgtcggatgg cttttccacc gaggggcggt gaccctcggc ctccatcagc gggggttcat
     1921 cacgctgttt caaatgaacg ccctcctgtc ctgtggaacg gtggcggtct tgggccacgt
     1981 gttcagcgaa gccatcggcg atcggtggtc cgcgttgccg ctggccctgg gggcggccca
     2041 cagcgccaat ttctggtccc gcgcgtcgga atgctccgtc tacgcgctcc atttgttcgc
     2101 ggccgccttg gtgttttggg ccctgtggcg tgacgcccgt cgcccgtccg tttcaacgtt
     2161 ggccgcgctc ggggccgcgg tggccttcgc ggtcctgacc cacatctcgg cggttttctg
     2221 ggtccccggc gttttcctcg cggtggggat ccaaaacggt cgttggcggc acgcggtggt
     2281 tttgggggcg gcctcggccg cggcggtgtt tttggttttc gccgcgacct atcgaatcgt
     2341 cgatttggaa agtttggcgg cgtggtaccg caaccaaggc agcgtgggtc ccctggccgc
     2401 ggggaccccg gacggtcttc ccgggtggcg ctgggccccg gacgcgccgt ttcttttccg
     2461 gggattggtc ggcgtcctct gggccgttcc cgcgggcggc gggctccttc cctgggccgc
     2521 gggggcggtg ggg
//
LOCUS       DAOQLS010000272         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25121_length_2533_cov_3.436642, whole genome shotgun sequence.
ACCESSION   DAOQLS010000272 DAOQLS010000000
VERSION     DAOQLS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25121_length_2533_cov_3.436642"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PK362_05940"
     CDS             <1..579
                     /locus_tag="PK362_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74406.1"
                     /translation="AGAVFAPEFLRDWVGPTVVPLWPWFSLMFVVPLLNILLTYGQTL
                     MQVRLSFLRANNRWLVAQAAGQMALSLAAVHFLKERAFIFGQVAAMVAVFPFVFRLLA
                     REQGLPARSVAGTVLRLLALGVPLAAVALTARWAGGVRGPVELVVAFGLWCGAYWAMI
                     YRWGVGPLERSVVARIASTVLRLESTAVGRPA"
     gene            576..1967
                     /locus_tag="PK362_05945"
     CDS             576..1967
                     /locus_tag="PK362_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74407.1"
                     /translation="MTVRTLERPIAGRPGRGRWRERWLAVRSPVVLWAMVLSTHGLAL
                     RMSDLRSAGGREGEAFAVLVLLALGSLLPLSVWACWKGDRLSPTGEPPPEDVLDRTHR
                     FFAFWALGASVGVALHWHAKWGLLGGEPFGGWNHLRNAWIHQPPVLPPWRRIESVAGH
                     LLSSAGQAGLFFSAEALFRRGGRRALGYFLAFAALVFLYALAMVSRSALMADLILVFL
                     AWSLARRRRSGTVAFRRGAGAALVLAGLSLVFVRSFLHDVISYSPGTGTERAASDARS
                     LEIYENGFADELPVVRDPAPDAFRAGVRRAWPSANVILLYLNHGLFNFAEVLGTDRRG
                     PPVVFGFVHHWKSLLFLAPRSTGTPPVRPYGRGGLTLPGAAYHDFGVGGVVAAALILG
                     SGLALAEGLLRRGGVFTGPGLILFVASGLTTGVSGIFVGPSVAVFPVVLFGVTVFGAP
                     AFRPRGGPGKGST"
     gene            1964..2527
                     /gene="gmhB"
                     /locus_tag="PK362_05950"
     CDS             1964..2527
                     /gene="gmhB"
                     /locus_tag="PK362_05950"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNC74408.1"
                     /translation="MTGRPAVFFDRDGTLNVEKNYVHAPAEWAWLPGAVDALRRVGEL
                     GLTAVVVTNQSGVGRGYYDRSAVTALHDWVAAGLSTQGISVAGFYFCPHVEADRCDCR
                     KPAPGLLTRAARELGLDLDRSFMIGDKAADVAAARAAGVTPILVLTGYGASERAAVPV
                     GVAVADDVVGAVRWIEGRVQNGERGLS"
BASE COUNT          274 a          783 c          937 g          539 t
ORIGIN      
        1 gcgggggccg tgttcgcccc ggaattcctg cgggactggg tgggtccgac ggtcgttcca
       61 ttgtggccct ggttttccct gatgttcgtc gtccccctgc tcaacatcct cctgacgtac
      121 gggcaaacgc tcatgcaggt ccgtctctct tttctccgcg cgaacaaccg ttggctcgtg
      181 gcccaggcgg ccggccaaat ggccctgtcc ctggccgcgg ttcatttcct gaaggagcgg
      241 gctttcattt tcggacaagt cgcggcgatg gtcgcggttt tcccgtttgt tttccgtctt
      301 ctggcccggg agcagggcct tccggcgcgc tccgtggccg ggacggtcct tcgacttctt
      361 gcgctcggcg ttcccctggc cgccgtcgcc ctgacggccc ggtgggcggg gggggtccgg
      421 gggccggtgg aattggtcgt cgccttcggc ctttggtgcg gcgcctattg ggccatgatt
      481 tatcggtggg gagtgggccc cctggagcgg tcggtcgtcg cgcgaatcgc gtcgaccgtc
      541 cttcggttgg aatccaccgc ggtggggagg cccgcttgac ggtccggacc ctggagcgtc
      601 cgatcgccgg tcggccgggc cggggccggt ggcgggaacg gtggcttgcg gtgcggtcgc
      661 ccgtcgttct ctgggcgatg gtcctgtcga cccacggact ggctcttcga atgtcggatt
      721 tgcggagcgc gggcggccgg gagggggaag ccttcgcggt tctggttctc ctcgccctgg
      781 gctcgctgtt gcccttgagc gtatgggcgt gttggaaagg cgaccggctt tcaccgaccg
      841 gcgaaccgcc ccccgaagac gttctggatc ggacccatcg tttcttcgcc ttctgggcgc
      901 tcggcgcttc cgtcggggtc gccttgcatt ggcacgccaa gtgggggttg ttgggcggcg
      961 aacccttcgg cgggtggaat catcttcgaa acgcctggat ccaccagccc ccggttttgc
     1021 cgccctggcg ccgaatcgag agcgtggccg gccacctgtt gtcgagcgcc ggacaggcgg
     1081 gcttgttctt ttccgcggag gccctctttc gacggggggg gcgaagggcc ctgggatact
     1141 tcctggcctt tgcggccctg gttttcctct acgcgctggc catggtgtcc cgcagcgcgt
     1201 tgatggcgga cctgatcctg gtcttcctgg cgtggtcgct ggcccgccgc cgtcgatcgg
     1261 ggacggtcgc ctttcgtcgg ggcgccgggg ccgcgctggt gttggcgggg ttgtccctgg
     1321 tgtttgttcg atcgtttttg cacgatgtca tcagctactc cccggggacg gggacggaac
     1381 gcgcggcgtc ggacgcccgg agccttgaga tctacgaaaa cggcttcgcg gacgaattgc
     1441 cggtcgtccg ggacccggcc ccggacgcct ttcgcgccgg cgttcggcgc gcttggccga
     1501 gcgccaacgt gatcctcctt tacctgaacc acgggttgtt caatttcgcc gaggtgttgg
     1561 gcaccgaccg ccgcgggccg cccgtggttt tcggctttgt gcatcattgg aaaagcctgc
     1621 tctttctggc cccccgatcg acgggaacgc cgccggtgcg gccctacgga cgcgggggat
     1681 tgacgcttcc gggggcggcg taccacgatt tcggggtggg gggcgtggtc gcggcggctc
     1741 tgatcctcgg gtccgggttg gccctggccg aaggattgct tcgacggggc ggtgttttca
     1801 ccgggcccgg tttgatcttg ttcgtggcgt cgggcctcac caccggggtt tcgggaattt
     1861 ttgtgggtcc gtcggtggcg gtttttcccg tcgtcctttt cggcgtcacg gtttttggcg
     1921 ccccggcgtt tcggccaagg ggcggccccg ggaaaggttc gacgtgaccg gccggcccgc
     1981 cgtttttttt gaccgcgacg gcacgctgaa cgtggaaaag aattacgtgc acgcccccgc
     2041 cgagtgggcg tggcttcccg gggcggtcga cgccctgcgc cgggtgggcg aactgggatt
     2101 gaccgccgtg gtggtgacca atcaatcggg ggtgggccgg ggatactacg accggtccgc
     2161 ggtgacggcg ctgcacgact gggtcgcggc cgggctgtcc acgcagggca tttcggtcgc
     2221 cggattttat ttttgccccc acgtggaggc cgaccggtgc gattgccgaa agccggcccc
     2281 cggcttgttg acgcgggccg cccgggagtt gggcctcgat ttggaccggt cgttcatgat
     2341 cggggacaag gcggccgacg tggccgcggc gcgggccgcg ggcgtaacgc cgattttggt
     2401 gctgacgggg tacggcgcgt ccgaacgggc cgcggtcccc gtcggggtgg cggtggccga
     2461 cgacgtggtc ggcgcggtgc gctggatcga agggcgcgtt caaaacggag aaagagggct
     2521 ctcatgagaa tct
//
LOCUS       DAOQLS010000273         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25134_length_2532_cov_5.320145, whole genome shotgun sequence.
ACCESSION   DAOQLS010000273 DAOQLS010000000
VERSION     DAOQLS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25134_length_2532_cov_5.320145"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2202)
                     /gene="fusA"
                     /locus_tag="PK362_05955"
     CDS             complement(97..2202)
                     /gene="fusA"
                     /locus_tag="PK362_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNC74409.1"
                     /translation="MARQYPLSKVRNIGITAHIDAGKTTTTERVLYYTGRIHKIGEVH
                     EGTTTMDWMEQERERGITITSAATYCRWRDCQINIIDTPGHIDFTAEVNRSLRVLDGA
                     VVLLDGSQGVEPQSETNWRLADQYHVPRLIFANKMDKVGADFYDCEKSVAEKLGVKGI
                     PIQLPIGAEGSFKGIVDLVEMNAHVWSGEELGAAFEIVEIPADLKEKAKAYREHLIES
                     LADFDDEFATKFLEGKEISKEEIKRVLRKATITGKFFPMLCGSSFKNKGVQPMLDAVC
                     DYLPAPTDLPAKVGHEPGTEKELLREATDDGPFSALAFKIQSDPHVGKVTYFRVYTGV
                     IKSGSYVFNANTGNRERLGRILRMHANKREEIEQVNAGEIAAGVGLKAVKTGHTLCDE
                     ENPVILESMKFPEPVISVAIEPKSKADEEKMGVALARLEDEDPTFRVRTDTETNQTII
                     AGMGELHLDILVDRMKREFNVQANVGQPQVAYRETFRKKVESEGKYIKQTGGRGQYGH
                     VWLELEPLAPGSGFEFVDKIKSGRVPREYIPAVEKGLRESMESGPMAGYPMVDFRATL
                     FDGSFHEVDSNEMAFKIAASLALKDGVRRAGAVLLEPIMRIDVTTPKDYLGDVMGDLM
                     RRRGKIESQDNKGNVTIIHGTVPLKEMFGYATTLRSLSQGRANYSMTPSHYEDAPSNV
                     QKEIVEKYQGAVSAGTLAR"
     gene            complement(2232..>2532)
                     /gene="rpsG"
                     /locus_tag="PK362_05960"
     CDS             complement(2232..>2532)
                     /gene="rpsG"
                     /locus_tag="PK362_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNC74410.1"
                     /translation="PMAVFTRAIENARPHLEVRPRRVGGATYQVPMEVRPERGATIAF
                     KWILQIARGKTGKPMADRLAEEILLCSKKEGAVMKKREDTHKMAEANQAFAHYRW"
BASE COUNT          374 a          741 c          818 g          599 t
ORIGIN      
        1 tcgtccccac gttcacgtgc ggtttcgtgc gctcaaattt gggttttgcc atggtcgttg
       61 ctccttgcgt tcagtgttct gcgagtcggg gtaaagctac cgcgccaggg ttccggcgct
      121 caccgcacct tgatactttt cgacgatctc tttttgcacg ttgctcggcg cgtcctcgta
      181 atggctgggc gtcatgctgt agttcgcccg gccctgggag agcgaccgca acgtcgtcgc
      241 gtaaccaaac atttccttca aggggacggt gccgtgaatg atcgtcacgt tgcccttgtt
      301 gtcctggctt tcgatcttgc cgcggcgccg catcaagtcg cccatgacat cgcccaggta
      361 atccttgggg gtggtcacgt cgatgcgcat gatgggctcg agcaggaccg cgcccgcgcg
      421 gcggacgccg tctttcaaag cgagggaggc cgcgatctta aaggccattt cgttggagtc
      481 cacttcgtgg aagctgccgt caaaaagcgt cgcccggaag tcgaccatgg ggtaaccggc
      541 catgggaccg gattccatgg attcccgcag gcctttttcc acggcgggga tgtattcccg
      601 gggcacgcgg ccgcttttga tcttgtcgac aaattcgaag ccgctgcccg gggccagggg
      661 ttccagctcg agccacacgt ggccgtattg gccgcggccg cccgtctgct tgatgtattt
      721 gccttccgac tccaccttct tgcggaaggt ttcgcggtag gccacctggg gctggccgac
      781 gttggcctgg acgttgaatt cccgtttcat gcggtcgacg agaatgtcca ggtgcaattc
      841 gcccatgccg gcgatgatcg tttggttcgt ttccgtgtcg gtgcggacgc ggaaggtcgg
      901 gtcttcgtct tccaagcggg ccagggccac gcccatcttt tcctcgtcgg ccttggactt
      961 gggttcgatg gcgaccgaga tgaccggctc ggggaacttc atggattcca aaatgaccgg
     1021 gttttcctcg tcgcagagcg tgtggccggt cttgacggcc ttgagcccga cgccggcggc
     1081 gatttcgccc gcgttgacct gttcgatttc ctcgcgcttg ttggcgtgca tgcggaggat
     1141 gcggcccagc cgctcgcggt tgccggtgtt cgcgttgaac acgtaggagc cggacttgat
     1201 gacgcccgtg tagacgcgga aataggtgac tttgcccacg tgggggtcgc tttgaatctt
     1261 gaaggccagg gccgagaacg gtccgtcgtc ggtggcttcc cggagcaatt ccttctcggt
     1321 gcccggttcg tggccgacct tggccggaag gtccgtcggg gcgggcaggt agtcgcacac
     1381 cgcgtccaac atgggttgca cgcctttgtt tttgaaggag gaaccgcaga gcatggggaa
     1441 gaatttgccg gtgatcgtgg ccttccgcag gacgcgcttg atctcctctt tggagatttc
     1501 tttgccttcc aaaaatttcg tcgcgaactc gtcgtcgaaa tccgccaggg attcgatcag
     1561 gtgctcgcgg taggccttgg ctttttcctt caggtccgcc gggatctcga cgatctcgaa
     1621 ggcggcgccc agttcctcgc ccgaccagac gtgggcgttc atctccacca aatcgacgat
     1681 gcctttgaag gagccttcgg ccccgatggg cagctggatc ggaatgccct tcacgccgag
     1741 cttttcggcc acggactttt cgcagtcgta gaaatcggcg ccgactttgt ccattttgtt
     1801 cgcgaaaatc agacggggca cgtggtactg gtccgccagg cgccagttgg tttccgattg
     1861 gggctcgacg ccctgggaac cgtccaacag caccaccgcg ccgtccaaga cgcgcaagga
     1921 gcggttcact tccgcggtga aatcgatgtg gcccggggtg tcgatgatgt tgatttggca
     1981 gtcccgccag cggcagtagg tcgcggccga ggtgatggtg atgccgcgct cgcgctcctg
     2041 ctccatccag tccatggtcg tcgtgccttc gtgcacctcg ccgatcttgt ggatgcggcc
     2101 ggtgtagtac aacacgcgtt cggtcgtggt ggttttcccc gcgtcgatgt gcgcggtgat
     2161 gccgatgttc cggactttgc ttaaagggta ctgacgggcc atgacggtct cgatttcctt
     2221 ttttttaaat tctaccagcg gtagtgggcg aaggcctggt tggcttcggc cattttgtgc
     2281 gtgtcttcgc gcttcttcat gacggcgcct tcttttttgc tgcagaggag gatttcctcg
     2341 gcgaggcggt ccgccatggg cttgccggtc ttgccccggg cgatctgcaa aatccacttg
     2401 aacgcgatgg tggcgccgcg ctccgggcgc acttccatgg ggacctggta ggtcgcgccg
     2461 ccgacgcggc gggggcggac ttccaggtgg gggcgggcgt tttcgatggc ccgggtgaac
     2521 accgccatgg gg
//
LOCUS       DAOQLS010000274         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25259_length_2526_cov_3.059085, whole genome shotgun sequence.
ACCESSION   DAOQLS010000274 DAOQLS010000000
VERSION     DAOQLS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25259_length_2526_cov_3.059085"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK362_05965"
     CDS             <1..532
                     /locus_tag="PK362_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74411.1"
                     /translation="GTGNESPVAALAADGFGRLRLAGASASSNFPMTVNAPQPLLAGG
                     TDGVLATFASLYPLPGGGGAPAVAAPPADAEFRLGDVYVYPNPSSGVPPRVHIEVGLA
                     DQVRVRVFNALGETVSDAVLTDAPAIVDDGQGAQYAYEPTLDAQLPSGIYTVSIVASK
                     RDHEDLRARAKWAVVR"
     gene            565..1455
                     /locus_tag="PK362_05970"
     CDS             565..1455
                     /locus_tag="PK362_05970"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC74412.1"
                     /translation="MKKWTVAILMAVAGSLSAAERPGAAFLSMGVGARAAALSSAYTG
                     VADDTSALYWNPAGLAGLTHREGLLSYAASFSDQRHNFAGYAQPTQRGAFGLGAVYLG
                     QGRLEGRGADRQKTGSFGAADTAFIVGAARRLSPGLSAGANLKLIRETIGSETAQGMA
                     LDLGATYRPPVRWLQTGAAVRNLGPGLSFAREKFALPATLTAGAAARLSNLGLASFDI
                     DYKLVEKKAEAKAGFEWSPGSAFALRAGYASPLAGAGVGASGFVWGLGVAHGRYRLDY
                     AMVPGEALGDTQQVSLRVGF"
     gene            1508..1795
                     /locus_tag="PK362_05975"
     CDS             1508..1795
                     /locus_tag="PK362_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74413.1"
                     /translation="MNPTAPPDPLVCHRCRRPLASGEGRPVALFYRLAAALSLGPVHA
                     GMWLWTDLNNPHCRSCRRWVSFLALVLATMALALAGWGFRWAFREKLLFNG"
     gene            complement(1813..2454)
                     /gene="can"
                     /locus_tag="PK362_05980"
     CDS             complement(1813..2454)
                     /gene="can"
                     /locus_tag="PK362_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNC74414.1"
                     /translation="MNPVDRLLQNNRRWAQKVQRQDPTFFKKLSRQQRPHYLWIGCSD
                     SRVPANQITGLLPGDVFVHRNVANLVVHTDLNCLSVIQFAVDVLKVKHIIVCGHYGCG
                     GVGAALRKDSLGLADNWIRHVQDVAHLHRDELSRLPTQNARLNRLAELNVTAQLINLC
                     GTTVVQSAWKRRQPLSVHGWIYGIADGIIKDLGLSARGPGDLPAPGDRRLFPG"
BASE COUNT          401 a          768 c          870 g          487 t
ORIGIN      
        1 cggaacgggc aacgaatccc ccgtggccgc cctggcggcg gacgggttcg gccgacttcg
       61 cttggcgggc gcctcggcct ccagcaattt ccccatgacg gtgaacgcgc cccagccgct
      121 cttggccggg gggacggacg gcgttttggc gaccttcgcc tccctgtatc ccttgcccgg
      181 gggcggcggc gcaccggccg tggccgctcc gcccgccgac gccgagttcc gtttggggga
      241 tgtgtacgtg tatcccaatc cgtcctccgg ggttccgccc cgggtgcaca tcgaggttgg
      301 gctggcggat caggtgcgcg ttcgggtgtt taacgccctg ggcgaaacgg tgtcggacgc
      361 ggtcctgacg gacgcgcccg cgatcgtgga cgacggccag ggcgcccaat acgcctacga
      421 gccaaccttg gacgcccaat tgccatcggg catttacacg gtgtccatcg tcgcttccaa
      481 acgcgaccac gaggatctgc gggcgcgggc gaagtgggcg gtggtgcgat gaacggcgac
      541 ggacggacgg agaggagagc gaccatgaaa aaatggaccg tggcgatttt gatggcggtg
      601 gcggggtcgt tgtcggcggc ggaacgtccg ggggcggcgt tcctgtccat gggcgtcggg
      661 gcgcgggccg cggccttgag ctcggcctac accggggtgg cggacgacac ttccgctttg
      721 tactggaacc ccgcgggact ggcggggttg acccatcggg aaggcttgct gtcctacgcg
      781 gcgagttttt ccgaccaacg tcacaacttc gcgggttacg cccagccgac ccaacggggg
      841 gccttcggtc tcggtgcggt ctatctgggg caggggcgtt tggaggggcg gggggccgat
      901 cgacagaaaa ccggttcttt cggcgcggcg gacacggctt ttatcgtcgg agcggcccgc
      961 cgtctttcgc cgggcttgag cgccggggcc aatctcaagc tgatccggga aaccatcggg
     1021 tcggaaaccg cccaaggaat ggctctggac ctgggggcca cctatcggcc gccggtgcgc
     1081 tggttgcaaa cgggggcggc ggtccgaaac ctgggccccg gcctctcctt cgctcgggaa
     1141 aaattcgccc tgccggccac cctcacggcc ggagcggcgg ctcgtttgtc aaacctgggg
     1201 ttggcgtctt ttgacatcga ttacaaactg gtggagaaaa aagccgaagc caaggccggg
     1261 ttcgaatggt cgcccggatc ggcttttgcc cttcgggccg gctacgcctc gcccctggcg
     1321 ggcgcgggcg tcggcgcgtc ggggttcgtg tgggggttgg gcgtggccca cggtcgttac
     1381 cgattggact acgccatggt tccgggcgag gccctggggg acacccagca agtcagcctg
     1441 cgggtcggct tctaaatacg attccagggt cccgcggggg gcgcgtcttt tatatcatcc
     1501 gtcggtaatg aatccgacgg ctcctccgga cccgcttgtt tgccaccgtt gccgtcgccc
     1561 cctggcgtcc ggcgaggggc ggcccgtcgc cctcttctac cgactggcgg cggccctctc
     1621 cctggggccc gttcacgcgg gcatgtggtt gtggacggat ttaaacaacc cccattgtcg
     1681 atcctgtcga cgctgggtat cgtttttggc cctggtcctg gcgacgatgg ccttggcttt
     1741 ggcgggatgg ggatttcggt gggcgtttcg ggaaaagctc ttgttcaacg gctgagggga
     1801 acccccgccg tttcagccgg gaaacagtcg acggtcgccg ggcgccggca aatccccggg
     1861 gccccgggcg ctgaggccca aatctttgat tattccgtcg gcgatgccgt aaatccatcc
     1921 atgaaccgat aacggctgac gccgtttcca ggccgactgg acgacggtgg tgccgcagag
     1981 gttgattagt tgagccgtta cgttcagttc cgccagccgg ttgagccggg cgttttgagt
     2041 cggcaatcgg ctcagttcgt cccggtgcag gtgggccacg tcctgcacgt ggcgaatcca
     2101 attgtccgcc aaccccaggg aatccttccg aagagccgct cccacgcccc cgcacccgta
     2161 gtgcccacat acaataatgt gtttgacttt caagacgtcc acggcgaatt gaatcaccga
     2221 taaacaattc aagtccgtgt ggaccaccag gttcgccacg ttgcggtgga caaacacgtc
     2281 ccccgggaga agcccggtga tttgattggc ggggacccgg ctgtcggaac agccgatcca
     2341 gaggtaatgg ggtcgttgtt ggcgggagag cttcttaaaa aacgtcggat cctgccgctg
     2401 aaccttttgg gcccaacggc ggttgttttg aagcagtcgg tcgacggggt tcatggatgt
     2461 tttaaaaaaa atcggaaagg gagggattcg aaccctcggt acgggagaac ccgtacgccg
     2521 gatttc
//
LOCUS       DAOQLS010000275         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25299_length_2524_cov_3.338194, whole genome shotgun sequence.
ACCESSION   DAOQLS010000275 DAOQLS010000000
VERSION     DAOQLS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25299_length_2524_cov_3.338194"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..2249
                     /locus_tag="PK362_05985"
     CDS             309..2249
                     /locus_tag="PK362_05985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC74415.1"
                     /translation="MRFAAEGILVFAFLLGAAALGEGVLPRRWRPEPFLERAAVALVI
                     GQAVVSFGLMVLGLLGGFYRPLLAAVYLTLAAGGVWRFHGKARTAFARATAAATRPVG
                     PVGFFLLFVLFAGLGRALIATAAPATDWDSLAYHLAFPRIFLTEGRLLSLPWSINAHY
                     PLNAEMLYTLGLAVRGDVFCHWLNFAHGVALAAVVFSLGARFFSRGAGALGAALLVVQ
                     PVFQRVIGNASTDLAVALPLVLAVSCAAKAASAPEEGARSWGLAGLLTGLAASTKLTG
                     LWMGAALMGVLLIRAFLGGPHRPGALRRFGGEALAFLFGMAAAGFPWYLRNFWTTGNP
                     VWPYAAAWLGGGPGAEAAWARISASVTERVPFTPGTLLSLPWKLWSLADLFKYRPYFL
                     TTPFFVLLFLRPFRRPAPSPGQKTVLGALGFFALLWLAVYPAWRYALPAAAWMAVFVG
                     GFWAEVFQRPRARWVGAPLLALALIPLGDLSVNAESFPFWGVRSQAAPTLTSRERYCQ
                     LTLGDPYTLAQEANALLPADARVLLYKDVRGFYLDRPYAWGDPLNPGVLDFNALKTPT
                     ELWHALRRMGFTHIVYNPFIEPTRGDRAYYDRADRLMADFLSRYGRALSIRGGGGLFA
                     ISAASADGEETAAGRSPPPSKI"
     gene            2260..>2524
                     /locus_tag="PK362_05990"
     CDS             2260..>2524
                     /locus_tag="PK362_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74416.1"
                     /translation="MNWRSWRVVGLYAACLALALARSDRKSSRPSTRPAAAKPGSAPA
                     FVSDGAIAVVDLRGPIALGMGGGLRDGTADGVLRRLRELREQSE"
BASE COUNT          268 a          831 c          866 g          559 t
ORIGIN      
        1 ggcttcgtcg gcccgggtcg ggggagcgcc tctgaaaacc ctcgcccggc aatgcggcgc
       61 cgacgaagcc gccgtgtttc gcgccgcccg ggaccagcga cgccgcatcg agcggactct
      121 ccttcccacg ggaccggcgg gccgatgagc gcgggggggg ttttagctcc tcccccgtct
      181 cccggttttt tcgccccccc gtcgcccttt cgctggcctt cctctatcta caaattcaat
      241 ccccgttttt tttgaaatat cttttcaccc accccggctc tttgaccggg gccgcggttc
      301 tcgagccgat gcgttttgcg gccgagggaa ttctcgtttt cgcgtttctg ttgggcgccg
      361 cggcgctggg ggagggggtt cttccccgtc ggtggcggcc ggagccgttc ctcgaacggg
      421 cggcggtcgc cctggtgatc gggcaggccg tcgtgtcgtt cggcttaatg gttttgggat
      481 tgctcggcgg tttttaccgg ccgctcctgg cggccgttta tctgaccctg gccgccgggg
      541 gggtgtggcg gttccacggg aaggcccgga cggcgttcgc tcgggccacc gccgcggcga
      601 cgcgtcccgt gggccccgtt gggtttttcc ttctctttgt ccttttcgcc gggttgggac
      661 gggcgttgat cgcgaccgcg gcgcccgcca ccgactggga ttccctcgct taccacctgg
      721 cttttccgag aatcttcctg acggagggac ggctgctttc actgccctgg tccatcaacg
      781 cccattaccc gctgaacgcc gagatgcttt acaccttggg cctggcggtg cggggcgacg
      841 tcttttgcca ttggctcaat ttcgcccacg gggtggcctt ggccgccgtc gtgttctccc
      901 tgggggcgcg attcttctcc cgaggggccg gggctctcgg cgcggccctg ttggtggtgc
      961 agccggtttt tcagcgggtg atcggaaacg cttccacgga cctggccgtg gcccttccgc
     1021 tcgttctggc ggtgtcctgc gcggcgaagg ccgcgtccgc ccccgaggaa ggcgcgcgtt
     1081 cctggggact ggccggcctt ttgacggggc tggccgcctc gaccaaactc acgggtcttt
     1141 ggatgggcgc cgccctgatg ggcgtactct tgatccgggc gtttctgggc gggccccatc
     1201 ggcccggcgc ccttcgccgc ttcggggggg aggcgctggc cttcctgttc ggaatggccg
     1261 cggccgggtt cccctggtac cttcgaaatt tctggacgac gggcaacccc gtctggccct
     1321 acgccgccgc ttggctcggc ggcgggcccg gggcggaggc ggcctgggcc cgtatttcgg
     1381 cctccgtgac ggagcgcgtt cccttcacgc cgggcacact gttgtccttg ccctggaaat
     1441 tgtggtccct ggccgatctt tttaaatacc gaccttattt cttgacgacg cctttttttg
     1501 ttttgctttt tctccggccg ttccgccggc cggcgccgtc cccggggcaa aaaacggtcc
     1561 tgggggccct gggatttttc gccctcctgt ggctggccgt ctatcccgcc tggcgttacg
     1621 ccttgccggc ggccgcctgg atggccgtct ttgtcggcgg tttttgggcg gaggtttttc
     1681 agcgcccccg ggctcgctgg gtcggggccc cgctcctggc cctggcgttg attcccctcg
     1741 gggacctgtc ggtcaacgcc gaatcttttc ccttctgggg cgtgcgctcc caggccgcgc
     1801 cgacgttgac ctctcgggaa cgctattgcc aattgacgct gggcgatccg tacacgctgg
     1861 cccaggaggc caacgccctt cttccggcgg acgcccgcgt gttgctttac aaagacgttc
     1921 gcgggtttta tctcgaccgc ccctacgcct ggggcgatcc gctgaatccc ggggtcctgg
     1981 attttaacgc cttgaaaacg ccgacggagt tgtggcacgc ccttcggcgg atgggtttca
     2041 cccacattgt gtacaatccc ttcatcgagc ccacccgggg cgatcgggcc tattacgatc
     2101 gggcggaccg gctgatggcc gactttttaa gccggtacgg ccgggcgctt tcaatacgcg
     2161 gggggggcgg tcttttcgcg atttcggcgg cgtcggccga cggggaagaa acggcggcgg
     2221 gtcggtctcc accgccgtcc aaaatttagg ggaggcgaca tgaattggcg atcgtggcgg
     2281 gtcgtcgggt tgtacgcggc gtgtttggct ctggccttgg cgcgttcgga tcgaaaatcg
     2341 tcgcgcccgt cgacgcggcc ggcggcggcg aagccggggt ccgcgccggc cttcgtctcg
     2401 gacggcgcca tcgccgtggt ggatctgcgg ggccccatcg ccctgggcat gggcggcggc
     2461 ctgcgggacg ggaccgccga cggggtgctt cgacggcttc gggaattgcg ggagcagtcg
     2521 gagg
//
LOCUS       DAOQLS010000276         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25317_length_2523_cov_3.614668, whole genome shotgun sequence.
ACCESSION   DAOQLS010000276 DAOQLS010000000
VERSION     DAOQLS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25317_length_2523_cov_3.614668"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK362_05995"
     CDS             complement(<1..396)
                     /locus_tag="PK362_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNC74417.1"
                     /translation="MRIVLGMTGASGSIFGVEFLRRATGEEVYLVLSRWGRSVLHQET
                     GLTAENLSTFSKKVFSNEDMNAPIASGSNPVDAVVILPCSMATLGKIAHGIGDNLITR
                     TAEVALKERRKLLLCLRETPLSTIDLENAH"
     gene            complement(421..1296)
                     /locus_tag="PK362_06000"
     CDS             complement(421..1296)
                     /locus_tag="PK362_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNC74418.1"
                     /translation="MAPPARVVDLKERLSAYARFIKIEHTLFSLPILLSGTLLARGSL
                     PSWRLTGLILLATLGARTLALALNRLIDVQIDARNPRTADRELATGALSLWDAFLVAI
                     AGLMVYIWAATKINSFCLIWSWVPALLFLIYPTLKRFTWLCHFGLGVTWAMAPLAGWF
                     AVNPGFAGSGPAWILAVFSFFWLAGFDIIYATLDEQFDRREGLFSVPARFGKRTALRL
                     SALTHLLAFAFLGLLFFFCLRGSAAAFLCLTAGFLLFLEHMLVDHVDLAFFKINVLTG
                     FVVLAMVFVGVRPDF"
     gene            1351..1977
                     /locus_tag="PK362_06005"
     CDS             1351..1977
                     /locus_tag="PK362_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74419.1"
                     /translation="MTGMSTVHKKPLVVFDIGNVLIAFSLERARRNFDRLEPGVGRRL
                     VDKIWNHRNGLGFERGRFTGKAFFQWIRRATGVRMTYRQFRGAFVDIFTPLEKNLKLL
                     ERLSATHDTALLSNINPIHWRHVMKEYPGLRKARWRFGSHRLGAMKPAARVYRAVSRK
                     TRTPFERMVYIDDRSDFVAAAKTLGIVARVYDRRRSLAAVLRSADVRP"
     gene            2059..>2523
                     /locus_tag="PK362_06010"
     CDS             2059..>2523
                     /locus_tag="PK362_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015193888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74420.1"
                     /translation="MTPIAPLVASLWHGLWEGAGREKTSDGNRWAGFLPWTILLIGLG
                     TTLGLWSLSLSSLPPNVSGVLLAQPRTAAGAFVALMFSNTPLWILLGGSFTTLALFGL
                     GWALTTAQRRAGLLAREMTRELRAAEAAARRASRAKSEFLANMSHELRTPLNS"
BASE COUNT          559 a          766 c          751 g          447 t
ORIGIN      
        1 gtgggcgttt tccaaatcga tggtggacag cggcgtttcc cgcaggcaca acaaaagctt
       61 gcgccgttcc ttgagggcca cttccgctgt gcgggtgatc aaattgtcgc cgatgccatg
      121 ggcaattttt ccgagcgtgg ccatggagca gggaaggatc accaccgcat cgacggggtt
      181 ggatccggag gcgatgggcg cgttcatgtc ctcgttggaa aacacctttt tggagaacgt
      241 ggaaagattt tccgcggtca atcccgtctc ttgatggagc accgatcggc cccagcggga
      301 caagaccaga taaacctctt ccccggtggc ccggcggagg aattccaccc caaaaatcga
      361 accggacgcc ccggtcattc ccaacacaat gcgcataacg actccttgtc ggccggaaaa
      421 ctagaaatcg ggacgaacgc ccacaaacac catggccagc accacaaacc cggtcaacac
      481 attgatttta aagaaggcca aatccacatg atccacgagc atatgctcca gaaataggag
      541 aaaccccgcg gtcaggcaaa gaaacgccgc cgcggatcca cgaagacaga aaaaaaagag
      601 gaggccgaga aacgcgaagg ccaaaagatg cgtgagcgcc gacagacgaa gggccgttcg
      661 tttcccaaac cgggccggga cggaaaacag gccttcccgg cgatcaaact gttcgtccag
      721 ggtggcgtaa atgatgtcga acccggccaa ccagaaaaaa ctgaaaacgg cgagaatcca
      781 agctgggccg ctccccgcaa acccgggatt gacggcgaac caccccgcca gcggcgccat
      841 ggcccaggtg acgcccagcc cgaaatggca caaccaagtg aagcgtttca gggtggggta
      901 gatcagaaac aacaaggcgg gaacccaact ccagatcagg caaaaagaat tgattttggt
      961 ggccgcccaa atgtacacca tcaagcccgc gatggccacg agaaaagcgt cccagaggct
     1021 cagagccccc gtggccaact cccggtcggc ggtccggggg tttcgggcgt cgatttgaac
     1081 gtcgatcagg cgattgaggg ccaaggccag ggttcgagcg cccagggtgg caagcaagat
     1141 gagacccgtc aagcgccagg agggaaggga gccccgggcc aacagcgtgc cggaaaggag
     1201 aatgggcagg gaaaaaaggg tgtgctcgat tttaatgaac cgggcgtagg ccgaaagtct
     1261 ttctttgagg tccacgaccc gtgcgggggg cgccataacc gtctccatat cgtcgctatt
     1321 ttagcacatc gagggggtct ccgtgttaaa atgaccggca tgtccaccgt gcacaaaaaa
     1381 cccctcgtgg tgttcgacat cgggaacgtt ttgatcgcct tcagcctcga acgcgcccgg
     1441 cggaatttcg accgcctcga acccggcgtc ggccgacgat tggtggacaa aatctggaat
     1501 catcggaacg ggttgggttt cgagcggggc cgattcaccg gaaaagcttt ttttcaatgg
     1561 attcgtcgag caacgggcgt gcgaatgacc tatcgccagt ttcgcggggc cttcgtggac
     1621 atcttcaccc ccttggaaaa gaacttgaaa ctcttggagc gcctgtcggc cacccacgat
     1681 acggcccttt tgtcgaacat caaccccatc cattggcggc acgtgatgaa agaatatccc
     1741 ggcctgcgaa aagcccgctg gcgattcggt tcccaccgac tgggagccat gaaaccggct
     1801 gcccgggttt accgggccgt ttcccgaaag acccggaccc ctttcgagcg catggtttac
     1861 atcgacgatc gaagcgattt tgtcgccgcg gccaaaaccc tgggcattgt cgctcgggtt
     1921 tacgaccggc ggcgatccct ggcggcggtc cttcggtccg ccgatgtccg cccgtaaccc
     1981 ccgccgcgcc tcatctttta gccaaagaag ccgatagtgt tcgggagagc ccctcgcccc
     2041 ggcgggggcc cgttcaccat gactcccatc gccccccttg tagcctccct ttggcacggc
     2101 ctttgggagg gggccggacg cgaaaaaacc tccgatggaa atcggtgggc cggcttcctt
     2161 ccttggacga tcctgttgat cggactggga acgaccctgg gcctttggtc cctgtccttg
     2221 tcgtccttgc cgccgaacgt atcgggggtt ctgctggcgc agcctcgaac ggcggcgggc
     2281 gccttcgtcg ccctgatgtt ttccaacacg cccctgtgga tcctgctggg ggggagtttt
     2341 acgaccctgg cgttgttcgg gttgggctgg gccttgacca ccgcgcaacg gcgggccggt
     2401 ctcctggccc gcgaaatgac ccgggaactg cgcgcggccg aggccgccgc ccgtcgcgcc
     2461 agccgggcca aaagtgaatt tttggccaac atgagccacg aattgcgaac cccgctcaat
     2521 tcc
//
LOCUS       DAOQLS010000277         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25568_length_2509_cov_4.703341, whole genome shotgun sequence.
ACCESSION   DAOQLS010000277 DAOQLS010000000
VERSION     DAOQLS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25568_length_2509_cov_4.703341"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PK362_06015"
     CDS             complement(<1..402)
                     /locus_tag="PK362_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC74421.1"
                     /translation="MATPLFEIKDLHVSVEGKKILNGLSLIVRAGETHAIMGPNGSGK
                     STLSYAVMGHPKYKIESGQVLYNGEDITAWPVNVRAVKGLFLAFQYPVAVPGVSVVNF
                     LRNILKNRRGQEIDLKTFRKELKENMKVLQMD"
     gene            complement(412..900)
                     /locus_tag="PK362_06020"
     CDS             complement(412..900)
                     /locus_tag="PK362_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC74422.1"
                     /translation="MRVTAMQEYGLRCMLQLATHNSDVPLAVREIAKRERLTPVYVEK
                     ILVNLRRAGLVKSVRGVNGGYALTKPARDISVGIVLSSLGQVDLSHNLCSRFTGDSAA
                     CVHQGDCSIRPIWGLLTRYIYGFLEKINLEQLLQDEAHVIQDVDKLGAKPQSLSAALR
                     SH"
     gene            1303..1512
                     /locus_tag="PK362_06025"
     CDS             1303..1512
                     /locus_tag="PK362_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74423.1"
                     /translation="MPARNAQNPIKADKGSAAASGGWNVEAKGPVKSGSNVLHCPSCG
                     AEVPPKPGFRPSALKCPQCGAAMKK"
     gene            complement(1525..>2509)
                     /locus_tag="PK362_06030"
     CDS             complement(1525..>2509)
                     /locus_tag="PK362_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74424.1"
                     /translation="LGFFQSGFQFVADRYTYISAWPLVLLTSGGAWRWAARESRRREF
                     LWAFLGIATLLLTALTSRYARAWTDGESLWTWAVASRPQSYTAQAGLGSYWIQRSLPE
                     KALPCFERSVELNPGFAEGYLKEGEALEMLGRDSETPALYEKARALNPGWWEPYLKLG
                     DAHRASGDLFQAEAMYRAAAQLKRGDAEILCRLGVTLMAQGKWAEARGPLFQAVRANP
                     GDPHAFNDLGIVFSNLGDAAVSEKNFQRALELSPDFAEARVNYGFLLLKAGRTEDAEG
                     QFRKALEEKKDFPEALAGLGYCAMARGNGTEAKTLLEKSRRLKPSLSPPGK"
BASE COUNT          474 a          759 c          762 g          514 t
ORIGIN      
        1 gtccatctgg aggaccttca tgttttcctt cagttccttt cggaacgtct tcaagtcgat
       61 ttcctggccc cgccggtttt tgaggatgtt ccgcaaaaag ttgacgacgc tcacgcccgg
      121 aacggccacg gggtactgga aggccagaaa gagacccttc accgcgcgca cgttgaccgg
      181 ccaggccgtg atgtcctcgc cgttgtaaag cacctggccc gattcgatct tgtatttggg
      241 gtgccccatg acggcgtagg acagggtgct tttgccggag ccgttgggcc ccatgatggc
      301 gtgggtttcc ccggcgcgaa cgatcaggga aaggccgttc aggatttttt tcccttcgac
      361 ggaaacgtga aggtctttga tttcgaaaag cggcgtcgcc atgggaaact cctagtggga
      421 ccggagcgcc gccgacaggg actgcggctt ggcgcccagc ttgtccacgt cctggatgac
      481 gtgggcttcg tcctgcaaca attgttcgag gttgatcttc tccagaaatc cgtaaatgta
      541 gcgggtgagc aagccccaga tggggcggat actacagtcc ccctggtgca cgcaggcggc
      601 ggaatcaccg gtgaagcggg agcagaggtt gtggctcaga tccacctggc ccagggagga
      661 gaggacgatg cccacgctga tgtcccgggc gggcttggtg agggcgtagc cgccgttgac
      721 gccgcgcacg cttttgacca gcccggcgcg gcggaggttc accaggattt tctcgacgta
      781 aacgggggtc aggcgttccc gcttggcgat ctcccggacg gccaggggca cgtccgaatt
      841 gtgggtcgcc aactgcaaca tacaacgcag tccgtattcc tgcatggccg tgactctcat
      901 gattccccct aatttgtccc gcgggtcaat tttgttttga cggcgcgatg tccgcccgtc
      961 ttggatcggc ctgatcttgg gcccgttgaa atcgttgatc tttgtgggcg agaaggcgtc
     1021 ggcccgaggg ccgcggcaag ggtcattaag ggcgccggcg tcgggccata atatgactgt
     1081 cttgtcatat ttattatacg ataccttaca attttgtcaa gtatagaaaa ttggtgtttt
     1141 ccgtctaaaa atccccccga aacgaataac ccttgaattc tccggggggt tcgtctattc
     1201 tatgggttca ggaacgacgc cgatttcagg ttgggccgaa gcggccgtcg tggggcgggg
     1261 atttccctga aggtcaacga tccacttttc gatggaggat ttatgccggc acgcaacgct
     1321 caaaatccga tcaaagccga taaagggtcc gccgccgcga gcgggggttg gaacgtcgaa
     1381 gccaagggtc ccgtaaaatc gggctccaac gtcctgcatt gcccgtcctg cggcgcggaa
     1441 gttcccccca aacccgggtt ccgccccagc gccctcaagt gcccccagtg cggggccgcc
     1501 atgaaaaaat aattccggac cggctcattt ccccggcggg gaaagtgacg gtttaagtcg
     1561 gcggcttttt tccaacaggg ttttcgcctc ggtcccgttc ccccgggcca tcgcgcagta
     1621 tcccaggccc gccagggcct cgggaaaatc ctttttctcc tccagggctt ttcgaaattg
     1681 tccctcggcg tcctccgttc gtcccgcttt caacaacagg aacccgtagt tcacccgggc
     1741 ctccgcgaag tccggcgaaa gttccaaggc ccgctgaaag ttcttttccg ataccgcggc
     1801 gtcgccgagg tttgaaaaca cgatgcccag gtcgttgaag gcgtggggat ctccggggtt
     1861 ggcccgaacg gcctggaaga gggggccccg ggcctcggcc cattttccct gggccatgag
     1921 cgtcacgccg agacggcaaa ggatttccgc gtctccccgc ttgagttgcg cggcggcccg
     1981 atacatggct tcggcctgaa aaaggtcgcc ggaagcccgg tgggcgtcgc ccaatttcaa
     2041 atagggttcc caccagccgg ggttcagggc tcgggctttt tcatagaggg ccggcgtttc
     2101 ggaatcccgc cccaacatct ccaaggcctc cccctccttt aagtacccct ccgcaaaccc
     2161 gggattcaat tccacgctcc gctcaaaaca aggcagggct ttctccggca ggctccgctg
     2221 gatccaatag gaacccagac ccgcctgggc cgtgtaggat tggggtcggg aagccacggc
     2281 ccaggtccag agggattccc cgtcggtcca ggcccgggcg tagcggctcg tgagggccgt
     2341 caaaagtaac gtcgcgatcc ccaggaaggc ccagaggaat tcccgccttc ggctctcccg
     2401 ggccgcccaa cgccaggcgc cgcccgaggt caaaaggacc agcggccaag cggaaatgta
     2461 ggtgtagcga tccgccacga actgaaagcc gctttggaaa aatcccagg
//
LOCUS       DAOQLS010000278         2508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25605_length_2508_cov_3.547085, whole genome shotgun sequence.
ACCESSION   DAOQLS010000278 DAOQLS010000000
VERSION     DAOQLS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25605_length_2508_cov_3.547085"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PK362_06035"
     CDS             complement(<1..884)
                     /locus_tag="PK362_06035"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNC74425.1"
                     /translation="MDATLRDDVRTLTTWLGRVIREQQGETFFTKLERLRQLAKATRA
                     RPGASSRAALRAFVDRLTPREAQNMARAFTLYFQLVNLAEEHHRIERLRDREREAGAP
                     PLSVEKALSDLRRAGVPRARIARAVANLRLEPVFTAHPTEAKRRVVLRHLLRMAEGWE
                     EHRRSDRSPSEKRRSENALLESLEALWQTQQVRQRRVTVEDEVRNVLFFLIGTVPRAA
                     AEFWDAFTEAAACLAPGAVPSPRLLSFGSWVGGDRDGNPSVTPDVSRWTLAEQRRTAL
                     EFYRNAVRDLQSLLTSSSL"
     gene            982..1236
                     /gene="rpsP"
                     /locus_tag="PK362_06040"
     CDS             982..1236
                     /gene="rpsP"
                     /locus_tag="PK362_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNC74426.1"
                     /translation="MVRLRLQKVGRPKTPHFRIVAIDSRKARDAAGLEVLGHYHPKDK
                     TNRITVNAERLKYWLSKGAQASDTLRTALRNAGALGPSAN"
     gene            1250..1489
                     /locus_tag="PK362_06045"
     CDS             1250..1489
                     /locus_tag="PK362_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005026147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNC74427.1"
                     /translation="MASALDWVLSVAKNLVQKPDDVQARWAEAERTVELRVAPEDRGK
                     IIGRRGKTIDSLRIVANAAFGGGDDARIDIKLLEE"
     gene            1517..2215
                     /gene="trmD"
                     /locus_tag="PK362_06050"
     CDS             1517..2215
                     /gene="trmD"
                     /locus_tag="PK362_06050"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010269647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNC74428.1"
                     /translation="MRVDVLTLFPRMFEGVFGESLLAKARTKGLADLRVHDIRAFADN
                     KHRTVDDRPYGGGPGMVIQAEPVHRALKSVGAAGKGRNKPFVIYLSPQGRPFTQALAD
                     RLAKKKRLVLLCGHYEGVDERIFEWIDLEVSLGDVVLTGGEIPAMAVVDAVVRKIPGT
                     VKEADSLAWDSFADGWRGRLDCPHYTRPAEWRGRKVPPVLLGGDHKAIAAWREAASAA
                     ATKKKRPDLLKIKK"
     gene            2223..>2508
                     /gene="rplS"
                     /locus_tag="PK362_06055"
     CDS             2223..>2508
                     /gene="rplS"
                     /locus_tag="PK362_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNC74429.1"
                     /translation="MNPILEKVESAFLKKNPPAFRVGDVVRVDIKIVEGESERVQAFE
                     GVVIRKRGHGIASTFTVRKMSFGVGVERTLPLHSPRIESLKVLRSGKVRRA"
BASE COUNT          469 a          787 c          827 g          425 t
ORIGIN      
        1 agagaggaag aggtcaagag ggactgaaga tcgcggacgg cgttgcggta aaactccagg
       61 gcggtgcgcc gttgctcggc cagggtccac cgggaaacgt cgggggtgac ggaagggttg
      121 ccgtcccggt cgccgccgac ccaggagcca aacgatagca accggggcga ggggaccgcg
      181 cccggggcca agcaggccgc ggcttcggtg aaggcgtccc aaaattccgc cgccgcccgg
      241 ggcacggtgc cgatcaggaa aaagagaacg ttccgcactt cgtcttccac cgtcacgcgc
      301 cgctggcgca cctgctgggt ttgccaaagg gcttccaggg attccaacag ggcgttttcc
      361 gaccggcgct tttccgacgg agatcggtcc gatcggcggt gctcctccca cccttcggcc
      421 atgcgaagaa gatgacggag gacgacgcgg cgtttcgctt cggtggggtg ggccgtaaag
      481 acgggttcca gtcggagatt ggcgacggcc cgggcgatgc gggcccgggg cacgcccgcc
      541 cggcggaggt ccgacagggc tttttccacg gagaggggcg gagccccggc ctcccgctcc
      601 cggtcgcgca ggcgctcgat gcggtggtgt tcctcggcga ggttcaccag ctgaaagtaa
      661 agcgtgaagg cccgggccat gttttgggct tcccgggggg tcaaacggtc cacgaaggcc
      721 cgcaacgccg cccggctgga ggccccgggt cgcgcccggg tagccttggc caattggcga
      781 aggcgctcca atttggtaaa aaaggtctcg ccctgttgtt cccggatgac ccggcccagc
      841 caggtggtga gggttcgaac gtcgtcccgc agcgtcgcgt ccatagcggt ttattttatg
      901 aattttaaag gtttttcgct tgacagcggg tcccgccctg aggtaaaata gcccgaattt
      961 aatcgagagg atcccacaca catggttcgc cttcgtcttc aaaaagtcgg gcgtccgaag
     1021 accccgcatt tccgcattgt cgccatcgac agccgcaagg cccgcgacgc cgccgggttg
     1081 gaagttcttg gtcactacca ccccaaggac aaaaccaacc gcatcaccgt caacgccgag
     1141 cgcctcaagt attggttgtc caagggcgcc caggcttccg acacccttcg caccgcgttg
     1201 agaaacgccg gcgcgctggg cccgtccgcc aactaagctc ttttttccca tggcgtccgc
     1261 cctcgactgg gttctttccg tcgcgaaaaa cctggtccag aaaccggacg acgttcaggc
     1321 ccgctgggcc gaggccgaac ggaccgtgga gctgcgcgtc gctccggagg accggggcaa
     1381 gatcatcgga cggcggggca agaccatcga ttccctccgc atcgtggcca acgccgcctt
     1441 cggcggcggc gacgacgccc gcatcgacat caagctttta gaggaataag ccgggttccc
     1501 ggcggacgcg tggaccgtgc gcgttgacgt tttgacattg ttcccccgga tgttcgaggg
     1561 ggtcttcggc gaaagcctgc tggccaaggc tcgaaccaag ggtctggcgg acctccgggt
     1621 ccacgacatc cgcgccttcg ccgacaacaa gcaccgcacc gtggacgacc ggccctacgg
     1681 gggcggaccg ggcatggtga ttcaggccga acctgttcac cgggcgttga aatcggtcgg
     1741 ggcggcggga aaaggccgaa acaaaccttt tgtgatttac ctgtcccccc agggacgtcc
     1801 cttcacccag gccctggcgg atcggctggc gaaaaagaag cgcctggtgc tgttgtgcgg
     1861 tcactacgaa ggcgtggacg aacggatttt tgagtggatc gacctcgaag tgtccctcgg
     1921 ggacgtcgtc ttgaccggcg gggaaattcc cgccatggcg gtcgtggacg cggtggtgcg
     1981 gaagattccc ggcacggtga aggaagccga cagtctggcc tgggattcct tcgcggacgg
     2041 ttggcggggg cggttggact gcccccacta cacccggccc gccgagtggc gcgggcggaa
     2101 agtgcccccc gtgctcttgg gcggggacca caaagccatc gcggcctggc gggaagcggc
     2161 ctcggcggcg gccaccaaaa agaaacgccc ggatttattg aaaattaaaa agtgagattg
     2221 ccatgaaccc cattcttgaa aaagttgaaa gcgccttttt gaaaaagaac ccgcccgcct
     2281 tccgcgtggg cgacgtggtc cgcgtcgaca tcaaaatcgt cgaaggcgag agcgaacgcg
     2341 tccaggcctt tgaaggcgtc gtgatccgca aacgcggcca cggcatcgct tccaccttca
     2401 ccgtgcgcaa gatgtctttc ggcgtcggcg tcgagcggac cctcccgctg cacagccccc
     2461 gcatcgaaag cctcaaggtg ttgcgctcgg gcaaagtccg ccgcgcca
//
LOCUS       DAOQLS010000279         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25697_length_2503_cov_3.756944, whole genome shotgun sequence.
ACCESSION   DAOQLS010000279 DAOQLS010000000
VERSION     DAOQLS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25697_length_2503_cov_3.756944"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>2503
                     /locus_tag="PK362_06060"
     CDS             22..>2503
                     /locus_tag="PK362_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74430.1"
                     /translation="MTSAVPLQSAMAGLVQKTQTTGRGASGASTSQTGSTNLDLTDFL
                     PIFSTLNNLNEKLLAEVEAVLASVSGPQPSGISGALSSILQGINRMLDLKTKLAGTIA
                     GGGLKGVPKQYTESTTQNTYAIVAGQAVLMNASTNSDTFTGFGAHDPHPVTTTHTTSR
                     TDYSYNDVGLLIGAMALSDSRTTNTVLSDGSPQVVVSTARTRSVYTVINGQAQVREST
                     TNSHSDGSGGASITDSQSTTYYRYDREGHLIGAFGQGEGTTQQLVGNHGGTATQITTS
                     KTTQHYGVFWGQAMVTETITEANTVSNGGTVNTASRSTVKNDYNSFGILVGASGQTHA
                     ETTMEVLTTVQQDGNPKMDTLVQRTVSDTTNRYVIILGQAMVSQTLSHSSGQSGATAS
                     GSTSQINYVYNDLGQLIDAQGYSTSNSSTGVWSDLNADGRWDVGESFSTPGWAQTKNT
                     YRIIWGQAVVSQTKSISGSQTADSLMGSATQMDYVYDSRGVLMSVSTPTVYSQGKELV
                     WGKDKNGDGKWTRDLEDPDKDESVEQTTITSTSNVYDVIWGQAVLVGTVSNSIIQVPG
                     TTTYQYAAVNYQYNANGQMIGATGSGNSTSYTLTDDKHIIVTYTTSKNTYGIFAGQAM
                     VTKTESHTESYSNYQDASLVEAAERYGIFKMKPLPAMPGTGQGSIEEKFDRFVAESKD
                     LWTVKSQEFKQFSGLDKFDTSTAVSTSVDDNILLNTYNSLGQLIGASSKNTTIQNVKN
                     ADGSVVTSTVNTENRYVVLLGQARLLESFSLSTTRTDNSLTTTPNRVRYEYNDMGQLI
                     GAHTVHKDGEKGLRITYNADGTETKTEIV"
BASE COUNT          613 a          815 c          662 g          413 t
ORIGIN      
        1 gtctcgacca tcagcggtct cttgacgtcc gccgtcccgc tccaatcggc catggccggg
       61 ctggttcaga aaacccaaac gacgggtcgc ggcgcgtccg gggcgagcac gtcccaaacg
      121 ggatccacga acctggactt aacggatttc ctgccgatct tctcgaccct caacaacttg
      181 aacgaaaaac ttcttgccga ggtcgaagcg gtattggctt cggtgtcggg acctcaaccg
      241 tccgggatct ccggagcgct ctcctcgatc ttgcagggga tcaatcgcat gctcgacctg
      301 aaaacgaaat tggccgggac catcgccggc ggaggattga agggtgttcc caagcaatac
      361 accgaatcca cgacccaaaa cacctacgcg atcgtggccg gccaggccgt cttgatgaac
      421 gcctccacaa actccgacac cttcaccggg ttcggcgccc acgatcccca tccggtcacg
      481 acgacgcaca cgaccagccg caccgattat tcctacaacg acgtcggctt gttgatcggc
      541 gcgatggcgc tgtcggacag ccgcaccacc aacaccgtct tgagcgacgg atccccgcag
      601 gtggtggtga gcaccgcccg gacccggagc gtttacaccg tgatcaacgg acaggcccag
      661 gttcgggagt cgacgacgaa ttcgcactcg gacggttcgg gcggcgcgag catcacggac
      721 agtcaatcga ccacgtacta tcgttatgac cgggaaggcc atttgatcgg cgccttcggc
      781 caaggggaag gcacgaccca acaattggtg ggcaaccacg gcggaaccgc cacccaaatc
      841 accaccagca aaaccacgca acattacggc gtgttctggg gccaggccat ggtgacggaa
      901 acgatcaccg aggccaacac cgtttccaac ggcggcacgg tcaataccgc gtcccggtcc
      961 accgtcaaaa acgactacaa ctccttcggc atcctcgtgg gcgcttcggg ccaaacccac
     1021 gcggaaacga ccatggaagt gctgaccacg gtgcagcagg acggcaatcc gaaaatggac
     1081 acgctggttc aacgcaccgt gtcggacacg accaaccgct acgtgattat cctcggccag
     1141 gccatggttt cccaaaccct ctcgcattcg tccggccagt ccggagcgac cgcctccggt
     1201 tcgacctctc aaatcaacta cgtctacaac gatctggggc aattgatcga cgcccaaggc
     1261 tacagcacgt ccaattcgtc caccggggtg tggagcgacc tcaacgccga tgggcgttgg
     1321 gacgtgggtg agtctttcag cacgccggga tgggcgcaaa ccaaaaacac ataccgcatt
     1381 atttggggcc aggccgtcgt ctcccaaacg aaatccattt ccggttcgca aacggccgac
     1441 agcctgatgg ggtccgccac gcaaatggat tacgtgtacg attcccgcgg cgtgttgatg
     1501 agcgtctcca cccccacggt gtacagccaa ggcaaagaat tggtttgggg gaaagacaaa
     1561 aacggcgacg gcaaatggac ccgcgacttg gaagatcccg acaaggacga atcggtcgaa
     1621 cagaccacga tcacctcgac ctcgaacgtc tacgacgtca tctggggtca agccgtcttg
     1681 gtgggcaccg tttcaaacag catcatccaa gttcccggga ccacgacgta ccaatacgcc
     1741 gcggtcaatt accaatacaa cgccaacggc caaatgatcg gagccaccgg ctcgggcaat
     1801 tcgacgtcct acaccctgac cgacgacaag cacatcatcg tcacttacac caccagcaaa
     1861 aacacctacg gcattttcgc gggtcaggcc atggtgacca aaacggagtc ccacaccgaa
     1921 tcctattcga actaccaaga cgcctcgttg gtggaagccg ccgaacgcta cggcatattc
     1981 aaaatgaaac ccttgccggc catgcccggg acggggcaag gatccatcga agagaaattc
     2041 gaccgtttcg tggccgagtc gaaggacctt tggacggtga aatcccagga gttcaagcaa
     2101 ttctccggct tggataaatt cgacacgtcg accgccgtca gcacctcggt ggacgacaac
     2161 attctcctca acacctacaa ctccctgggg cagctcatcg gcgcttcctc caaaaacacc
     2221 accattcaaa acgtcaaaaa cgccgatggg tcggtggtga cgagcaccgt caacacggag
     2281 aaccgctacg tggtcctgtt gggccaggcc cggctcttgg aatcgttctc cttgagcacc
     2341 acccgaaccg acaacagcct gacgaccacc ccgaaccgcg tccgatacga gtacaacgac
     2401 atgggtcagt tgatcggcgc ccacacggtc cacaaagacg gcgaaaaggg ccttcgcatc
     2461 acctacaacg ccgatggaac ggaaaccaaa accgaaatcg tcg
//
LOCUS       DAOQLS010000280         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25821_length_2496_cov_3.562474, whole genome shotgun sequence.
ACCESSION   DAOQLS010000280 DAOQLS010000000
VERSION     DAOQLS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25821_length_2496_cov_3.562474"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PK362_06065"
     CDS             complement(<1..805)
                     /locus_tag="PK362_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNC74431.1"
                     /translation="MTSAAVKKEIERLREDIRRHDRLYYLDARPEISDVEYDRLMRRL
                     RDLEDAHPDLRTADSPTQRVSGTATTDFKPVRHAVPMLSLDNTYNPDELIEWRDRVAK
                     LLGPEEKPGYVVELKVDGLGLALLYEKGRLVRAATRGDGETGEDVTANARSIRAIPLR
                     LEGRSPARLEVRGEVFVTKKDFETYNRRIAAEDPAAAFANPRNFAAGSLRQKEARVTA
                     GRPLRYFVHSYGVVDGVDHEFHHDFLKDCRAWGLPVDGHTTRCASFEEVL"
     gene            complement(830..1282)
                     /gene="nusB"
                     /locus_tag="PK362_06070"
     CDS             complement(830..1282)
                     /gene="nusB"
                     /locus_tag="PK362_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNC74432.1"
                     /translation="MGLRRQAREAALQILYLADIANLPGDKAAKIIWAGSEAPPKSKN
                     FADELARGALAHRLALDAVIAKVAENWEIGRMAAIDRNILRLAAFELMHSLETPVSVV
                     IDEAVEIAKTYSTQDSGKFVNGILDKIKTERPAPPPGAETPEGQRELF"
     gene            complement(1282..1758)
                     /gene="ribH"
                     /locus_tag="PK362_06075"
     CDS             complement(1282..1758)
                     /gene="ribH"
                     /locus_tag="PK362_06075"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNC74433.1"
                     /translation="MSRRRAASPLTTASGRGRRFAVVTARFNADITESLRSACAATLR
                     AGGVTGGNLVEVYVPGTFELPWACQRLARTKKFDAVIALGCVIRGETPHDRYIAQATA
                     QGLMRVSLDTGVPVLFGVLTPLNLRQARARSGRGAGNKGAEVARAALDVAALPGAV"
     gene            complement(1760..>2496)
                     /gene="ribA"
                     /locus_tag="PK362_06080"
     CDS             complement(1760..>2496)
                     /gene="ribA"
                     /locus_tag="PK362_06080"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNC74434.1"
                     /translation="YPAAVICEIMNEDGTMSRLPALQKFSRRHRLAMVTIEDLIAYRR
                     RHEKLIRRLASVALPTRHGDFKLHLYEDVPTGEHHVAMVKGDVSGHKKVLVRVHSSCF
                     TGDVLHSYRCDCGEQLERALERIDKEGRGVLLYMHQEGRGIGLANKLHAYALQEAGLD
                     TVQANKKLGFAPDLREYGIGAQILSDLGLSSVRLLTNNPKKIVGIEGYGLTVTQRVPL
                     EIPSNRHNARYLAAKRKKLGHWLKGL"
BASE COUNT          417 a          763 c          858 g          458 t
ORIGIN      
        1 cgaggacttc ctcaaagctc gcgcaccggg tcgtgtgccc gtccacggga aggccccagg
       61 cccggcaatc ctttaaaaag tcgtggtgga attcgtgatc cacgccgtcg accacgccgt
      121 aggagtggac gaaataccga aggggccgcc cggcggtgac gcgggcctct ttttgacgaa
      181 ggctcccggc ggcgaaattg cgcgggttgg cgaaggcggc ggcgggatcc tccgcggcga
      241 tgcgccggtt gtaggtctcg aaatcctttt tggtcacgaa cacttcgccc cggacttcca
      301 gtcgggccgg agaacggcct tccaggcgaa gcgggatggc ccgaatggag cgcgcgttgg
      361 ccgtgacgtc ctccccggtt tccccgtccc cccgggtggc ggcgcggacc aggcggcctt
      421 tctcatagag cagggccagc cccaggccgt ccaccttcaa ctccaccacg taacccggtt
      481 tctcctcggg gcccaaaagc ttggcgaccc ggtcccgcca ttcgatcaac tcgtcgggat
      541 tgtaggtgtt gtccagggac agcatgggca cggcgtggcg aacgggcttg aaatcggtcg
      601 tggcggttcc ggacacccgc tgggtcggag aatcggcggt tcgaaggtcg gggtgggcgt
      661 cctccaggtc ccggaggcgg cgcatcaagc ggtcgtactc gacgtccgag atttcgggac
      721 gggcgtccag gtaatagagg cggtcgtggc gccggatgtc ctcgcggagg cgctcgattt
      781 cttttttgac ggcggcggag gtcatcggcg cgcgggggcg gcggagggat taaaagagtt
      841 cgcgctgtcc ttcgggggtt tccgctcccg ggggcggggc ggggcgctcg gttttgatct
      901 tgtccaaaat gccgttgacg aacttgccgg aatcctgggt cgaataggtt ttggcgatct
      961 ccacggcctc gtcgatgacg accgacaccg gggtttccag gctgtgcatg agctcgaagg
     1021 ccgccaagcg gaggatgttc cggtcgatgg ccgccatgcg gccgatttcc caattctcgg
     1081 ccactttggc gatgacggcg tccagggcca gacggtgggc cagggcgccc cgggccaatt
     1141 cgtcggcgaa attcttggat ttgggcggcg cctcggagcc cgcccaaatg attttggcgg
     1201 ccttgtcgcc gggcagattg gcgatgtccg ccagatacaa aatttgaagg gcggcctccc
     1261 gggcctggcg acgcaaaccc attagacggc tcccgggagg gccgcgacgt ccaaggcggc
     1321 gcgcgccact tcggcgcctt tgttgcccgc gccccgaccc gaacgggccc gggcctgccg
     1381 aaggttcagg ggcgtcagaa cgccgaaaag cacgggcacc ccggtgtcca gggacacgcg
     1441 catcaatccc tgagccgtgg cctgggcgat gtagcggtcg tggggggttt caccgcgaat
     1501 gacgcacccc aaggcgatca cggcgtcgaa ttttttggtc cgggccaggc gttggcaggc
     1561 ccaaggcagt tcaaaggttc ccgggacata aacctcgacc aggtttccgc ccgtcacgcc
     1621 gccggcccgg agcgtggccg cgcaggccga tcgaagagac tcggtgatgt cggcgttgaa
     1681 ccgggcggtg accacggcga aacggcggcc gcggcccgag gccgtcgtca atggggaagc
     1741 ggcgcgtcgt cgactcacgt caaaggccct tcaaccaatg gccgagtttt ttgcgtttgg
     1801 cggccagata gcgggcgttg tggcggttcg acggtatttc caagggaacc cgttgggtca
     1861 ccgtcaaccc gtagccttcg atgccaacga tttttttggg gttgttggtg agcagccgca
     1921 cgctggaaag gcccaaatcg gacaggatct gggctccgat gccgtactcc cgaagatcgg
     1981 gggcgaaacc caatttcttg ttggcctgca ccgtgtccag gccggcttcc tgaagggcgt
     2041 aggcgtgcag tttgttggcc aggccgatgc cccgtccttc ctggtgcatg tagagcagga
     2101 cgccccggcc ttccttgtcg atccgctcca gggcccgttc caactgctcg ccgcaatcgc
     2161 accggtaaga atgaagcacg tcgcccgtga agcaggagga atgaacccgc accaggacct
     2221 tcttgtggcc cgacacgtcc cctttgacca tggccacgtg atgctccccg gtgggaacgt
     2281 cttcgtacaa gtggagtttg aaatccccgt gccgggtggg aagcgccacc gaagccaggc
     2341 ggcggatcaa cttctcgtgc cgccggcggt aggcgatcaa gtcttcgatg gtcaccatgg
     2401 ccaggcggtg acggcgggaa aatttttgaa gggcgggcag gcggctcatg gtgccgtcct
     2461 cgttcatgat ctcgcagatc acggccgcgg ggtaca
//
LOCUS       DAOQLS010000281         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_25885_length_2493_cov_4.302297, whole genome shotgun sequence.
ACCESSION   DAOQLS010000281 DAOQLS010000000
VERSION     DAOQLS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25885_length_2493_cov_4.302297"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PK362_06085"
     CDS             <1..662
                     /locus_tag="PK362_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HNC74435.1"
                     /translation="PKQETVYSSDPVEMAKRWVAQGAQRLHVVDLDGAFTGHPANLDL
                     VYRIKDAVNVVVEFGGGLRDPETVKKVLDRGIGRAIVGTAILKYPQWIKTTLAEYPGR
                     LMASLDAVNGEVMIEGWQADSGHTLADVIKTIDNLGFREIVYTDIKQDGTLKGPNVKS
                     IGEVLAMTHMGVYASGGISSLDDLKALKALEAKGLKGAILGKSLYAGKINLPDALAIA
                     K"
     gene            659..1429
                     /gene="hisF"
                     /locus_tag="PK362_06090"
     CDS             659..1429
                     /gene="hisF"
                     /locus_tag="PK362_06090"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNC74436.1"
                     /translation="MKSGLTRRLIPCLDVDGGRVVKGTRFLNLRDAGDPVEVARRYNA
                     EGADELVFLDITASSQKRPILLDVVTRTAEQVFIPLTVGGGVRTVADVRTLLSAGADK
                     VSINTAGVQRPALFKEASRAVGSQCVVAAIDAKRTGRGRWEVFIHGGRTATGKNAVAW
                     ARETVRQGAGEILLTSMDADGTKAGYDTDLLRAVCRAVRVPVIASGGAGRPSHFADAF
                     RAGADAALAASLFHFRELSINRLRRYLAGRKIPIRRIP"
     gene            1426..1818
                     /gene="hisI"
                     /locus_tag="PK362_06095"
     CDS             1426..1818
                     /gene="hisI"
                     /locus_tag="PK362_06095"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNC74437.1"
                     /translation="MSLEWIESVKFDDKGLVPAVVQDDKDGTLLMVAYMNKEALRETV
                     ERRRGVFFSRSRNKMWRKGEESGNFQEVKSIALDCDKDCVLIRVNQIGGAACHTGRKS
                     CFFHHVTDKNEIDIRGEILFDPAKVYKK"
     gene            1819..2322
                     /gene="lepB"
                     /locus_tag="PK362_06100"
     CDS             1819..2322
                     /gene="lepB"
                     /locus_tag="PK362_06100"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNC74438.1"
                     /translation="MKRILYLLFLGALGAWVLRAYALEGIYIATGSMEPTLPVGLHVF
                     VNKVVYRVRAPQRGDIVLFPSPVEQKELVKRVIAVGGDTLRIERKQVILNGKPLEEPY
                     VRHTRAGELLEGDDLDMGTVPPGHLVLLGDNRDESGDSRDWKDAQGKPVYFIPVAAIK
                     GRVLGQS"
BASE COUNT          471 a          762 c          835 g          425 t
ORIGIN      
        1 acccgaaaca agaaacggtg tattcctcgg atccggtgga gatggccaag cggtgggtgg
       61 cccagggcgc gcaacgcctg cacgtcgtgg acctggacgg cgccttcacg ggccatccgg
      121 ccaatctgga cctggtgtac cggatcaagg acgcggtgaa cgtcgtcgtg gagttcggcg
      181 gcggcctgcg ggacccggaa acggtgaaaa aggttttgga ccgcggcatc ggccgcgcca
      241 tcgtggggac cgccatcttg aaataccccc aatggatcaa gacgaccctg gccgaatacc
      301 ccggacgcct catggcctcc ctcgacgccg tgaacggcga agtgatgatc gaaggctggc
      361 aggccgactc gggacacacc ctggccgacg tgatcaaaac catcgacaac ctgggtttcc
      421 gggaaattgt gtacacggac atcaaacagg acgggacgct caaggggccc aacgttaaat
      481 ccatcggtga agtgctggcc atgacccaca tgggcgtcta cgcctccggc gggatttcct
      541 ccttggacga cctcaaggcc ttgaaggccc tggaggccaa gggtctcaag ggcgccattc
      601 tcggcaaatc cctgtacgcg gggaaaatca atttgcccga cgccctggct attgcgaaat
      661 gaaatcgggg ctgacccggc gcctcatccc ctgtctggac gtcgacgggg gccgggtggt
      721 gaaaggcacc aggttcctga acctgcggga cgcgggcgat ccggtggaag tggcccggcg
      781 ctacaacgcc gaaggggccg acgagctcgt gtttttggac atcacggcct cctcccagaa
      841 gcggcccatc ctgttggacg tggtgacccg cacggcggaa caggtgttca tcccgctgac
      901 ggtcggcggc ggggtgcgga ccgtggccga cgtccgcacg ctgttgtccg cgggggcgga
      961 caaggtctcg atcaacaccg cgggggttca acgaccggcg ctttttaagg aagcctcccg
     1021 cgccgtgggt tcccagtgcg tggtggccgc catcgacgcg aagcggacgg ggcggggccg
     1081 ctgggaggtt tttatccacg ggggccggac cgccacgggc aaaaacgccg tggcctgggc
     1141 ccgggagacc gtgcgccagg gcgccgggga aatcctgctg accagcatgg acgccgacgg
     1201 caccaaggcg ggctacgaca cggatttgct ccgggccgtc tgccgggccg tccgggtccc
     1261 ggtcatcgct tccggcgggg ccggccgccc gagccatttc gccgacgcct tccgcgccgg
     1321 cgccgacgcc gccctggccg cgtccctgtt tcattttcgt gagttgtcca tcaaccgcct
     1381 tcggcgctac ctggccgggc ggaaaatccc catcaggaga atcccgtgag cttggaatgg
     1441 atcgagagcg ttaaatttga cgacaagggc ctcgtgcccg ccgtggtgca ggacgacaag
     1501 gacgggacgt tgctcatggt ggcctacatg aacaaagagg ccctgcggga aaccgtggag
     1561 cgccgccgcg gcgtcttttt cagccggtcg cgcaacaaga tgtggcgaaa gggcgaggag
     1621 agcggcaatt tccaggaagt gaaatccatc gcgttggatt gcgacaagga ttgcgtcctg
     1681 atccgcgtga accaaatcgg tggggcggct tgccacacgg gccgaaaatc ttgttttttc
     1741 caccacgtga cggacaaaaa cgaaatcgac atccggggcg aaattctctt cgatcccgcg
     1801 aaggtctata aaaaataaat gaagcggatt ttgtacctgt tgtttctggg cgccctgggc
     1861 gcgtgggtcc ttcgggccta cgccctggaa ggcatttaca tcgccacggg ctccatggaa
     1921 cccacgctcc ccgtcggctt gcacgtgttc gtcaacaagg tggtgtaccg ggtgcgggcg
     1981 ccccagcggg gggacatcgt tctgtttccg tcgcccgttg agcaaaaaga gctggtcaag
     2041 cgggtgatcg cggtcggcgg cgacacgctc cgcatcgaga gaaaacaggt gattctgaac
     2101 ggcaagcccc tggaggagcc ctacgtgcgc cacacccggg ccggcgagct tctggagggg
     2161 gacgatctgg acatgggcac ggttcccccg gggcatctcg tgctcctcgg cgacaaccgc
     2221 gacgagtccg gcgacagccg ggactggaag gacgcccagg ggaaacccgt ttatttcatc
     2281 cccgttgcgg cgatcaaggg aagggtgttg ggccagtcgt gatcactccg agcctgccgg
     2341 aattcaaacg cctggcccgg cgccacaacg ccgtccccgt ggcggtggag tgcctggccg
     2401 acgaagtgac gcccctgggt cttttccacg gcctgtatgc gaagagcccc caatgttttc
     2461 tcttggaaag cgtcgagggg ggcgaacacc tgg
//
LOCUS       DAOQLS010000282         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26020_length_2485_cov_4.746091, whole genome shotgun sequence.
ACCESSION   DAOQLS010000282 DAOQLS010000000
VERSION     DAOQLS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26020_length_2485_cov_4.746091"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK362_06105"
     CDS             complement(<1..294)
                     /locus_tag="PK362_06105"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNC74439.1"
                     /translation="MGVRVDAVSPDDVLRRVNEFLSHPRPRQIATANSLMILAAARHP
                     GLSHALAAADLVVPDSVGVVWAAARRGARLIQIPGVELMERMCAEAADRGWTVY"
     gene            complement(354..>2485)
                     /locus_tag="PK362_06110"
     CDS             complement(354..>2485)
                     /locus_tag="PK362_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC74440.1"
                     /translation="SAHAALDGADRAVAEAQERQARTQEELFRAQAQLDAQEEWEASD
                     VYARGVQSVLSAGLPGIHGPVGKLISVSTADEVAVRRALGVHVNDLVADTLEDARAAI
                     RYLTDGAHGRARFLVLDRLPAAPPAVAAPAGQKALLEMARADARFEPVVRHLLNGWFS
                     ADGALFGDGVLEGGSENAAGPVFDALRRENLQKEIAAHTAALAEVQRSKASLDEARRG
                     AAGELDAARRALEETRARQAVRLEERERVEKQIALAERERDVITAEMARARAEESQAQ
                     SSAAALEVRLEDLKTEEGRLRGEWANLQTSLQERQADNFAASSELAVARERAHGHEER
                     LRWKEKQLSDVAAERQTLSVAVEAKTQERSGSSTKVEEQKRIERESEARISELLVERK
                     AADEALEAVQGQRRVLAEKIQTVEKSLAELRRANDEMQQQLHEKKLQHGHAQFRIEAL
                     ETHLKDRYQMTIAEAREGHPAPTEPVPATELEKLRRRVEGLGPVNLAAPEEHAQLEER
                     YSFLLSQQQDLVKAKEDLLNTIQKINASTRLNLKETFDKVRENFKTIYSQLFPGGEAD
                     VRLTDENDVLNAGVEIYAQPPGKKLQNITLLSGGEKALTALSLLFAFYMVRPAPFAIL
                     DEVDAPLDEANVTRFVTLIKAFTEHAQFIVITHNKRTMETADTLYGVTMEVQGVSRLL
                     SARLKTLEDRRAAQSPEAVLASPGGAG"
BASE COUNT          375 a          821 c          830 g          459 t
ORIGIN      
        1 ataaaccgtc cacccgcggt ccgcggcctc ggcgcacatt cgttccatca attccacccc
       61 ggggatctgg atcagccggg cgccccgccg ggccgcggcc cagacgacgc cgacggaatc
      121 gggcacgacc aaatccgccg cggcgagggc gtgggagagg cccggatggc gcgcggccgc
      181 caggatcatc aaggaattgg cggtggcgat ttggcggggg cgcgggtggg acagaaattc
      241 gttcacccga cgcaggacgt cgtcggggga aacggcgtcg acgcggacgc ccaagacgga
      301 gacgcgggga acgggagccg ggcgcgggga gcggggagcc gccgccggca cggtcagccg
      361 gcgccgccgg gggaggccag aaccgcttcg gggctttggg cggcccggcg gtcttccaag
      421 gttttaagcc gggcggacag aaggcgcgaa acgccctgga cctccatggt cacgccgtac
      481 agcgtgtcgg ccgtttccat ggtgcgcttg ttgtgggtga tgacgatgaa ttgggcgtgt
      541 tccgtgaaag ccttgatcaa ggtgacgaac cgcgtgacgt tggcctcgtc cagcggcgcg
      601 tcgacttcgt ccaggatggc gaagggcgcc ggccgcacca tgtagaaggc gaacagcaac
      661 gacagggccg tgagcgcctt ctcgccgccg gaaagcaacg tgatgttttg cagctttttc
      721 cccgggggct gggcgtagat ctccacgccc gcgttcaaca cgtcgttttc atcggtcagg
      781 cgaacgtcgg cttccccgcc cgggaacaac tggctgtaga tggttttgaa attttcccgg
      841 accttgtcga aggtttcctt caggttgagc cgggtcgacg cgttgatttt ctgaatcgtg
      901 ttcaacaagt cttccttggc cttcaccagg tcctgctgtt gggacagcag gaagctgtaa
      961 cgctcctcca attgggcgtg ctcctcggga gcggccaaat tgacgggccc caggccctcc
     1021 acgcggcgac gaagtttttc caattccgtg gccggcacgg gctcggtcgg cgcggggtgg
     1081 ccttcccggg cttccgcgat cgtcatttgg tatcggtcct tgagatgggt ttccagggcc
     1141 tcgatccgga attgggcgtg gccgtgctgg agtttcttct cgtgaagctg ttgttgcatt
     1201 tcgtcgttgg cccgccgaag ttcggcgagg gatttttcga ccgtctgaat tttctcggcc
     1261 aacacgcggc gctggccttg gacggcttcc agggcttcgt cggccgcctt gcgctccacc
     1321 agcagttccg aaatccgggc ttcggattcc cgttcgatgc gtttctgctc ctccactttc
     1381 gtggaggaac cggaacgttc ctgggttttg gcttcgacgg cgacggacag cgtttggcgc
     1441 tcggccgcca cgtcggacaa ttgtttttcc ttccagcgca ggcgttcctc gtgcccgtgg
     1501 gcccgttccc gggccacggc caactcggag gacgccgcga aattatcggc ctgccgctcc
     1561 tggagcgacg tttgcaggtt ggcccattcg ccccgcaacc gcccttcctc ggtcttcaga
     1621 tcttccaagc ggacttccag agccgcggcg gaggactggg cctgggactc ttcggcgcgg
     1681 gcccgggcca tttcggcggt gatcacatcg cgctcgcgct cggccagagc gatttgcttt
     1741 tccacccgtt cgcgttcttc caaacgaacg gcctggcggg cccgggtttc ctccaacgcg
     1801 cgacgggcgg cgtccaactc gccggcggcc ccgcgtcggg cctcgtccaa agacgctttg
     1861 gagcgctgaa cctcggccaa cgccgccgtg tgggcggcga tttccttctg caaattttcc
     1921 cggcgaaggg cgtcaaagac gggccccgcc gcgttctcgc ttcccccctc caacacgccg
     1981 tcgccgaaaa gcgcgccgtc ggcggagaac cacccgttca agaggtgccg aaccacgggc
     2041 tcaaaccggg cgtcggcccg ggccatttcc aacaaagcct tctggcccgc cggcgcggcg
     2101 accgccggcg gcgccgccgg aagacggtcc aacaccagga accgcgcccg gccgtgggcg
     2161 ccgtccgtca aatagcggat ggcggcccgg gcgtcctcca gggtgtcggc caccaaatcg
     2221 ttgacgtgga cccccagagc gcgtcggacg gcgacttcgt ccgccgtgga caccgaaatc
     2281 aatttgccga ccgggccgtg gattccgggc aacccggcgg acaacacgga ttggacgccc
     2341 cgggcgtaga cgtcggaggc ttcccattcc tcctgggcgt ccaactgggc ctgggcccgg
     2401 aacaattcct cctgggtgcg ggcctggcgc tcttgcgctt cggccacggc ccggtccgct
     2461 ccgtccagag ccgcgtgggc cgacc
//
LOCUS       DAOQLS010000283         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26040_length_2484_cov_4.599424, whole genome shotgun sequence.
ACCESSION   DAOQLS010000283 DAOQLS010000000
VERSION     DAOQLS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26040_length_2484_cov_4.599424"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PK362_06115"
     CDS             <1..754
                     /locus_tag="PK362_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74441.1"
                     /translation="PTLDDYYVHVFGRNDHHARGAVEKLGAALERVRSRRVTAQRVGG
                     VSFLLFVGLLVGHAVNHDIPLFAASITGFAAAFLGIASLPRMRRLALQEAWHEYKEYF
                     FLLPLFLSISLLQKSGFFDIIGHLLHRGIEVLGPSHVAYGQFAFATVLSALLDNNVVA
                     DFAGRALQGLEVHLIHLFAMAQIAGYALGGCWTHVGSAQSVVAYAFIQKEIDPRYTPF
                     QWIKAMTPVILEIAVWMSIVVYGEGMMLEWLG"
     gene            1138..>2484
                     /locus_tag="PK362_06120"
     CDS             1138..>2484
                     /locus_tag="PK362_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC74442.1"
                     /translation="MVVSIPSSGERVADRSGAATGTASDIAKAGPDLTILDIANQAGK
                     NKVAINFMWVLITGFLVMFMQAGFAMVEAGMTRAKNAAHTMAMNFMIYPLGMLGFYVC
                     GFALMFGGLGAVGTLGGYGALNHEVSVTLFGKTFGLFGAKGFFLTGSTYDVALFTLFL
                     FQMVFMDTTATIPTGSMAERWKFSAFMIYGAFVGTILYPVYGNWVWGGGWLATLGVNF
                     GLGHGHVDFAGSSVVHLTGGVLAFVGAKIIGPRIGKYNSDGTPNAIPGHNIPMAIIGT
                     FILAFGWFGFNPGSTLAGTDLRTGVAAVNTMLASATGALSATLWMWLVRTKKPDPGMM
                     CNGMLAGLVAITAPCAFVNSLTASLIGLISGVLVIEAAFFVEGKLKIDDPVGAIAVHG
                     VNGAFGCLALGLFADGTYGDGWNGVAGPVKGLFYGDASQFMAQVIGVGANLVYVGVM"
BASE COUNT          408 a          802 c          751 g          523 t
ORIGIN      
        1 cccgactctc gacgattatt acgtacatgt cttcggccgg aacgaccacc acgcccgggg
       61 ggcggtggaa aagttgggcg ccgccctgga gcgggtgcgg tcccgccggg taacggccca
      121 acgggtcggg ggcgtgtcct tcctcctgtt cgtggggctt ctggtcggtc acgcggtcaa
      181 tcacgacatt ccccttttcg ccgcgtccat caccgggttc gcggcggcgt ttctggggat
      241 cgcctccctc ccccggatgc gacgcctggc gctccaggag gcctggcacg aatacaagga
      301 atacttcttc ctcctgcccc ttttcctttc catttccctg ctgcaaaagt ccggcttctt
      361 cgacatcatc gggcacctgt tgcatcgggg catcgaggtc ctcggcccct cccacgtggc
      421 ctacggccaa ttcgccttcg ccaccgtcct gtcggccctt ctggacaaca acgtggtggc
      481 cgatttcgcc ggccgggctc tccagggcct ggaggtgcac ctgatccacc ttttcgccat
      541 ggcccagatt gccgggtacg ctctgggcgg gtgctggacc cacgtgggtt cggcccagtc
      601 ggtggtggcc tacgccttca ttcaaaagga aatcgatccg cgctacaccc ccttccagtg
      661 gatcaaggcc atgacccccg tgattttgga aatcgcggtg tggatgtcca tcgtcgtcta
      721 cggcgagggg atgatgctgg agtggctggg ctgaccaccg cccgtcggta aaaaaagtcg
      781 ttgacatttt tttgaaatgt tgtaaaattt ccccaacgca cacagtggtg cgatgcagaa
      841 tggacattga agtccttctc cgaaatccgg ggaagggctt ttttatttga accaaacgag
      901 acggcgttgt ctcaacccga ggacggggga gacagcgcct tttttgtttc tccccttctc
      961 cggttgagcc gccctcgaac caaggaggac gtcatgttgt cacgaatgag acgttgggga
     1021 ttggggatca cgttggccct ctggggcctc acggccctcg ttccctttgc ggtacaagcg
     1081 gaagaaaaga ccccggcccc cgcggccgcg gccccggcgc cggtggaagc cagcgccatg
     1141 gtggtcagca tcccatcgag cggtgaacgc gtggccgatc ggtcaggcgc cgccacggga
     1201 acggcttcgg acatcgccaa agccggcccg gacctcacga tcctggacat cgccaaccaa
     1261 gccggcaaga acaaagtcgc catcaacttc atgtgggttc tgatcactgg attcttggtc
     1321 atgttcatgc aggcgggttt cgcgatggtg gaggccggca tgacccgtgc caaaaacgcg
     1381 gcccacacca tggccatgaa cttcatgatc tacccgctcg ggatgttggg cttctacgtc
     1441 tgcggcttcg ccctgatgtt cggcggcctg ggcgcggtcg gcaccctagg aggctatggg
     1501 gctctcaacc acgaagtgag cgttaccttg ttcggtaaaa ccttcgggct cttcggagcc
     1561 aaagggtttt tcctgacggg ctccacctac gacgtggccc tcttcaccct gttcctcttc
     1621 caaatggtct ttatggacac gaccgccacc atccccacgg gctccatggc ggaacggtgg
     1681 aaattctccg ccttcatgat ctacggcgcc ttcgtcggga cgatcctcta tcccgtctac
     1741 ggcaactggg tctggggcgg cggctggttg gccaccctgg gtgtgaactt cggcctcggc
     1801 cacggccacg tggacttcgc gggctcctcg gtcgtgcact tgaccggcgg cgtcctggcc
     1861 tttgtcggcg ccaaaatcat cgggcctcgc atcggaaaat acaactccga cgggaccccg
     1921 aacgcgatcc cggggcacaa catccccatg gcgatcatcg gcaccttcat cctggccttc
     1981 ggttggttcg ggttcaaccc cggctccacc ctggccggca cggaccttcg caccggcgtg
     2041 gccgcggtca acaccatgct ggcctccgcc acgggcgccc tgtcggcgac cctctggatg
     2101 tggctggtcc gcaccaaaaa acccgatccg ggaatgatgt gcaacggcat gttggcgggc
     2161 ttggtggcca tcacggcccc ctgcgctttc gtcaacagcc tcacggccag tttgatcggc
     2221 ttgatctccg gcgtcctggt cattgaagcg gccttctttg tggaaggcaa gctcaaaatc
     2281 gacgatcccg tgggcgccat cgcggttcac ggcgtcaatg gcgccttcgg ctgcttggcc
     2341 ctcggtcttt tcgccgacgg gacctacggc gacgggtgga acggcgtggc gggccccgtg
     2401 aagggcctgt tctacggcga cgcgtcccaa ttcatggcgc aggtcatcgg cgtgggcgcg
     2461 aacctggtgt atgtcggcgt gatg
//
LOCUS       DAOQLS010000284         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26085_length_2482_cov_4.242274, whole genome shotgun sequence.
ACCESSION   DAOQLS010000284 DAOQLS010000000
VERSION     DAOQLS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26085_length_2482_cov_4.242274"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..511
                     /gene="rbfA"
                     /locus_tag="PK362_06125"
     CDS             122..511
                     /gene="rbfA"
                     /locus_tag="PK362_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNC74443.1"
                     /translation="MWQRRIERVNELLLREISNYVLENQPPDMGFVTFTGVSVTEDLE
                     QARVYFSVMGTDAEKARSLDLLNAMRTDLTRRMRRLESLKRIPELHFEFDATPERAAR
                     VHEIIEHLHAPAPDPAPAKDRPPRKKT"
     gene            514..1506
                     /locus_tag="PK362_06130"
     CDS             514..1506
                     /locus_tag="PK362_06130"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNC74444.1"
                     /translation="MPLPPIPRSSQKAIGQILAAVKKHRTFFLTGHERPDGDTVGSEL
                     AFAGFLRRRGKKVTVANTGPVPALFDFLPGVRNIQTAARVPGRFDCAVVFECSGPDRM
                     GNIIDLKTQAGTVINIDHHAHHSRFGHINLINPKTSSNSEQLAYVFERARHTLNRAEA
                     TALYVGLVTDSGRFQHNCTTPESHAVAALLLRRGVDVAEVHRHVYGTRSVSALRLLAR
                     ALAGLRLELDQRVAVLSLTQKDFAETGAGEDDTEDIVNHGLLPPSVAATVFLCELPKG
                     GTKASLRGKGAVDLCRLAVSLGGGGHKNAAGVTLPVSVERATQTLLDALAESLR"
     gene            1508..2236
                     /gene="truB"
                     /locus_tag="PK362_06135"
     CDS             1508..2236
                     /gene="truB"
                     /locus_tag="PK362_06135"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNC74445.1"
                     /translation="MPSLLGRRVLSGLFPIDKPKGPTSHDAVLWARRVLNTADVGHCG
                     SLDPMATGLLLLVVGEARAQQNRFMSERKVYEGTVRLGGATDTDDTDGRPLPGPFPRS
                     VAEVSETEIRAALARFTGVFDQTVPTYSAVKVDGKPLYHWARKGVPVEKPVKSVEVHA
                     MDFISYTPPDLDFRVDCAKGFYVRALARDLGNALGTGGVLSRLCRESIGNFQRRRAYP
                     WVDRAALDPAVFEQSFIPIERLPD"
     gene            2236..>2482
                     /locus_tag="PK362_06140"
     CDS             2236..>2482
                     /locus_tag="PK362_06140"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNC74446.1"
                     /translation="MGRPVVLTLGTFDGVHRGHQALLAQARRRARTLGGEVLAVAFDR
                     PPRLFFHPEPPPALITTPEERAVLLRRFGADRVDRLAF"
BASE COUNT          433 a          873 c          757 g          419 t
ORIGIN      
        1 agtggagaag ggttacgagt gcggcatctc cctggcgaac tttcaggacg tcaaggtggg
       61 ggacaagatc gagttctacg tcatcgaaaa acaggcccgc aagctctaga ggggcgctcc
      121 catgtggcaa cgtcgcatcg aacgcgtcaa cgagctcctt ctccgggaaa tctccaatta
      181 cgtgctggaa aaccagcccc cggacatggg gtttgtgacc ttcacggggg tgagcgtgac
      241 ggaggacctc gaacaagccc gcgtctactt ttccgtgatg gggaccgacg ccgagaaggc
      301 ccggagcctg gatcttctga acgccatgcg cacggacctc acgcgccgca tgcgccgcct
      361 ggaaagcctg aaacgcattc ccgaacttca tttcgaattc gacgcgacgc ccgaacgggc
      421 ggcccgggtt cacgaaatca tcgagcacct tcacgccccg gcccccgatc cggccccggc
      481 caaagaccgc ccgccgcgca aaaaaacctg atcatgcccc tgccgccgat ccctcgatcg
      541 tcgcaaaagg ccatcgggca aatcctggcg gcggtcaaaa aacaccggac gttttttctc
      601 accggccacg agcgccccga cggggacacc gtggggtccg agttggcctt tgcgggtttt
      661 ctgcgacggc gcggcaagaa ggtcaccgtg gccaacaccg gcccggtgcc ggccctgttc
      721 gatttcctgc ccggcgtccg aaacattcaa accgcggccc gggtgcccgg ccggttcgac
      781 tgcgcggtgg tctttgagtg ctcggggccg gaccgcatgg ggaacatcat cgatttgaaa
      841 acccaggcgg gcaccgtcat caacatcgac caccacgccc accacagccg gttcggccac
      901 atcaatctca tcaatcccaa aacctcctcg aactcggaac aactggccta cgtcttcgaa
      961 cgggcccggc acacgttgaa ccgggcggag gccaccgcgc tgtacgtcgg cctcgtgacg
     1021 gattcgggac gctttcagca caactgcacg acgccggaaa gccacgccgt ggcggctttg
     1081 cttctgcgac ggggcgtgga cgtggccgag gttcaccgcc acgtgtacgg gacgcggtcg
     1141 gtttcggccc tgcgcctcct ggcccgggct ctggccggcc tgcggctgga actggatcaa
     1201 cgggtggcgg ttctctccct cacccaaaaa gatttcgccg aaaccggcgc cggggaggac
     1261 gacaccgaag acatcgtcaa ccacggcctc ctgccgccct cggtggcggc caccgttttt
     1321 ctctgcgaat tgcccaaggg ggggaccaaa gccagcctgc gcggcaaagg cgccgtcgac
     1381 ctgtgccggt tggccgtgtc cctgggcggg ggcgggcaca aaaacgccgc cggcgtcacg
     1441 ctcccggttt ccgtggaacg ggcgacgcaa accctcctcg acgccctggc cgaaagcctc
     1501 cggtaaaatg ccttcgcttc tcggccgccg ggtcctttcc ggcctctttc ccatcgacaa
     1561 acccaaggga ccgacgtccc acgacgcggt cctctgggcc cggcgggtcc tgaacaccgc
     1621 ggacgtgggc cactgcgggt ccctggaccc catggccacg ggactgcttc ttctggtcgt
     1681 cggggaggcc cgcgcccagc aaaaccgttt catgtccgag cgaaaagtct acgaaggcac
     1741 ggtgcgcctg ggcggggcca ccgacaccga cgacaccgac ggccgtcccc tgccggggcc
     1801 ttttccgcga tcggtcgccg aggtgtcgga aaccgaaatc cgcgccgccc tggcccgctt
     1861 cacgggcgtt tttgatcaga ccgtgcccac gtattcggcg gtcaaagtcg acggcaaacc
     1921 tctgtaccat tgggcgcgca aaggcgttcc ggtcgaaaaa ccggtcaaat ccgtggaagt
     1981 ccacgccatg gattttattt cctacacccc gcccgacctc gacttccgcg tggattgcgc
     2041 caaggggttc tacgttcgcg ccctcgctcg ggatctgggg aacgccctcg gcacgggcgg
     2101 cgtgctgtcc cgtctctgcc gggaatccat cggaaatttt caacgccgtc gggcgtatcc
     2161 ctgggtggac cgggccgccc tggaccccgc ggtgttcgaa caatcgttta ttcccatcga
     2221 acggctgccc gactgatggg tcggccggtc gtcctgaccc tcggcacttt cgacggcgtt
     2281 caccgagggc accaggccct gctggcccag gcccgtcgcc gggcccggac cctcggcggc
     2341 gaggtgctcg ccgtggcctt cgatcggccg ccaagacttt tcttccatcc cgaaccgccc
     2401 cccgccttga tcacaacccc ggaggaacgc gccgtccttc tccgccgctt cggcgcggac
     2461 cgggtggacc ggctggcctt cg
//
LOCUS       DAOQLS010000285         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26233_length_2474_cov_3.143034, whole genome shotgun sequence.
ACCESSION   DAOQLS010000285 DAOQLS010000000
VERSION     DAOQLS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26233_length_2474_cov_3.143034"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..659)
                     /locus_tag="PK362_06145"
     CDS             complement(156..659)
                     /locus_tag="PK362_06145"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNC74447.1"
                     /translation="MNIVLTGFMTTGKSSVGRLLAERLGYAFFDTDDMIEKQTGLSIA
                     EIFSKGTEASFRELETRTVNLLAVLDHAVIATGGGVPLRAGNMDELEKNGKIVCLTAA
                     PATVLERLRSDAGTRPLLQGAEPLQRIETLLADRKTAYARCHASVATDGLTVEQVADK
                     VRRAVGL"
     gene            complement(676..1839)
                     /gene="aroC"
                     /locus_tag="PK362_06150"
     CDS             complement(676..1839)
                     /gene="aroC"
                     /locus_tag="PK362_06150"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNC74448.1"
                     /translation="MIRYLTAGESHGPCLTAVLEGVPAGLKITEDYLNQELHRRQYSF
                     GRGERLQYIEKDTVKILSGVRWGETLGSPIAFRIENRDWENWEKIMSVDAADKAEKFR
                     LTRPRPGHADLVGVLKYDRQDTRDILERASARETAARTAVGSVCKRLLEELGVKIYSW
                     VVEIGGVVAKTDGLSIEESFSLAEKSPVRCPDKAAEEKMVEAIKEAKTNGDTLGGVYT
                     VVVTGCPVGLGSHTHWDRKLDGKLAQAILSIQAHKGVEIGHGFGLARSKGSEVHDELF
                     YQAGRGFYRKSNNAGGIEGGMTNGEPIVVRAAVKPLASLRRPLKSVNIQTKEEMTAEI
                     VRSDVCPVASAAVVGETVVAIALAEAVSEKFGSDSIREMRDNRDRYTAHLLDY"
     gene            complement(1836..>2474)
                     /locus_tag="PK362_06155"
     CDS             complement(1836..>2474)
                     /locus_tag="PK362_06155"
                     /inference="COORDINATES: protein motif:HMM:NF036850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74449.1"
                     /translation="VPVANRVGAEHDGWRVANTDVEGFDEDLRALGVSVRGKNVLLWG
                     AGGAARAVLGALALHPDSVPARVDVLNRTPARVKTLLDDLNARGSVGLRRLAIHAPSA
                     AESARAVSNADVLINATSAGDDSLVGSAPLKRTVVAYDLTYRKPTAFMEAVRRSGGTA
                     HGGLGMLVNQGARAFEFWFEGYLKKKVDYSFLDLRRVMRDAAEKALKERETA"
BASE COUNT          399 a          798 c          815 g          462 t
ORIGIN      
        1 cggtttggcg tagcgccggg ccacgggggc cgcgctcacg accagcgccc gccgtccctt
       61 gatcaggggg cccagggacc ggcccaaact ttccaggggc gaccccacga cgatctcgta
      121 ggggcggccg ttgttcaggg gaacggcgac ggttctcaca accccaccgc ccgccggact
      181 ttgtcggcca cctgctccac cgtcaacccg tccgtggcga cggacgcgtg gcagcgggcg
      241 taggccgttt tacggtccgc caaaagggtt tcgatccgtt gaaggggttc ggcgccctgc
      301 agaaggggcc gggtgccggc gtccgagcgc aagcgctcga gcaccgtggc gggagcggcg
      361 gtgaggcaaa cgattttgcc gtttttctcc aattcgtcca tgttgccggc gcggaggggc
      421 acgccgcccc ccgtggcgat cacggcgtga tccaggacgg ccaggaggtt caccgtgcgg
      481 gtttccaatt cgcggaagga ggcctccgtt cccttggaaa aaatctcggc gatcgagagg
      541 ccggtctgct tttcgatcat gtcgtcggtg tcgaaaaagg cgtacccgag gcgctccgcc
      601 aggaggcgcc ccaccgacga tttcccggtc gtcatgaaac cggtgaggac aatgttcacg
      661 tcgcgccggg gtccttcagt agtcgaggag atgggccgtg taacggtccc ggttgtcccg
      721 catttcccgg atcgagtccg acccgaattt ttccgagacg gcttcggcca gggcgatggc
      781 gacgacggtt tcgccgacca ccgcggcgct ggccacggga cacacgtcgg agcggacgat
      841 ctccgccgtc atttcctctt tggtttgaat gttgacggat ttgaggggac ggcgcaggga
      901 cgccaggggt ttgacggccg cccggacgac gatgggttcg ccgttggtca tgccgccttc
      961 gatgccgccg gcgttgttgg atttgcggta aaaaccccgg ccggcttggt aaaagagttc
     1021 gtcgtggacc tcggacccct tggaccgggc cagaccgaag ccgtggccga tctccacgcc
     1081 cttgtgggcc tggatggaca ggatcgcctg ggccagcttg ccgtccagtt tccggtccca
     1141 atgggtgtgg ctgccgaggc ccacggggca gccggtgacg acgacggtgt agacgccgcc
     1201 cagggtgtcg ccgttggttt tggcttcttt aatcgcctcc accattttct cctcggccgc
     1261 tttgtccggg caccgcacgg gggatttttc cgccaacgaa aaggactctt cgatggacag
     1321 gccgtcggtt ttggcgacga cgccgccgat ttcaaccacc caggaataaa tttttacgcc
     1381 cagctcctcc aacaggcgct tgcagacgga ccccacggcc gttcgggccg cggtctcccg
     1441 ggccgaggcc cgttccagga tgtcccgggt gtcctgccgg tcgtatttga gaacaccgac
     1501 caaatcggcg tggccggggc gggggcgcgt gaggcggaat ttttccgcct tgtcggccgc
     1561 gtccaccgac atgatctttt cccagttttc ccagtcgcgg ttttcgatgc ggaaggcgat
     1621 gggcgagccc agggtttccc cccagcggac gccggagagg atcttgaccg tgtctttttc
     1681 gatgtactgc aggcgttcgc cgcgcccgaa actgtactgg cggcggtgaa gctcttgatt
     1741 caaatagtct tcggtgattt taagtccggc ggggacgcct tccaaaacgg cggtcaggca
     1801 gggcccgtgg gactctccgg cggtcaggta acgaatcacg ctgtctccct ctccttcaag
     1861 gctttttccg cggcgtcgcg catgacacgc cgcaaatcca gaaatgaata gtctaccttt
     1921 tttttcaggt atccctcgaa ccagaactca aaggcccggg cgccttggtt cacgagcatg
     1981 ccgagcccgc cgtgggccgt tcccccgctc cggcgaacgg cttccatgaa agccgtcggc
     2041 tttcggtaag tgaggtcgta ggcgacgacc gtccgtttca agggggcgga cccgaccaag
     2101 gaatcgtccc cggcggaggt ggcgttgatg agtacgtcgg cgttggaaac ggcccgggcg
     2161 gattcggccg ccgagggggc gtggatggcc agccgacgga gaccgacgga cccccgggcg
     2221 ttcaagtcgt ccaacagggt tttcacccgg gccggcgttc ggttcagaac gtcgacccgc
     2281 gccggcacgg aatccggatg aagggccaag gcgcccagca cggcccgggc cgcccccccc
     2341 gcgccccaca gaagcacgtt cttgccccgg accgaaaccc ccagggcgcg caaatcctcg
     2401 tcaaatccct cgacgtcggt gttggcgacc cgccaaccgt cgtgctcggc cccgacccgg
     2461 ttggcgacgg gcac
//
LOCUS       DAOQLS010000286         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26240_length_2473_cov_3.942514, whole genome shotgun sequence.
ACCESSION   DAOQLS010000286 DAOQLS010000000
VERSION     DAOQLS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26240_length_2473_cov_3.942514"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2473)
                     /locus_tag="PK362_06160"
     CDS             complement(<1..>2473)
                     /locus_tag="PK362_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74450.1"
                     /translation="GLGAFREPAQPLDGESAGRAAERLARPVSAPNPTVATAGDLRER
                     PDAMDDLLGAVREGLRLDPAMPLENLPAPFLGALDRWNARGEYLRAPRSGNVELVRQK
                     IRLLSFVHLPSGKPLPVRLEWRAGLGLVLWAGDELFFDLHRHRERVLAGAPGMYSPQA
                     ASTIATLADTLAVQAKRQRLNALLGALLNADRPDRNLLREIDALSETGVPLPVSTMGE
                     DRMLFTTPRSGRPLVIQTDALSGEAQTVRPRWLPGWGPVILVETEQGVLAYGLERYRG
                     APEKKGRVNADYLDAFASVPEADRELPRRLAFAGAVFDFARRDPASVSAEEAAATVGA
                     LQRAPVVFNVPSSGTLPLRYPRAAAGESAARKQVLFLERATPGPNRATFEFDGTAGVR
                     FSLAPASGGNAVWYAPEVLRDGRRMAYLRPTTEKGLEWRRRRAVADRWRELAAEALAP
                     PFLWALREPDAFAEKRKAFNGLGAVLRTNAAGELSFDFPDGPGGVVEIHSPKDALAPN
                     ADHRVTLEWVWGAGLLMFVEPADGGPVSVFGFGGVRRARPGGVGRPRMSRVGEGKFPT
                     AADARAALERNWVLALRRATVLTGGESVGEADVRRLFETFNRLPVADREVVVGVNNKL
                     YITDPFDGGTVHVRLMGPTGTVYEVRLRTHRRLGPVAVFVLRRAPRPALAPVRVLWDL
                     SDALGRSHRRGGEAVDMFPGVRLESVRGLSGVLDRRAEEIVVDPVYGRRPPLGVPANA
                     DLIRFVENAVAEARGEGPAGRVRASLLSEGSEILGGTGPLADLLVSVESRSPADDAVL
                     REEVYSVFRQRLNGRYGYRVGREGSV"
BASE COUNT          448 a          878 c          810 g          337 t
ORIGIN      
        1 agacggagcc ctcccggccg acccggtaac cgtagcgtcc gttgagtcgc tgacgaaata
       61 cgctgtacac ttcctcccgc aaaacggcgt cgtccgccgg cgaccgggat tcgaccgaca
      121 ccaagaggtc ggccagaggg cccgtgcctc cgaggatttc ggaaccctcc gataaaagag
      181 aggcgcgaac gcgtccggcg gggccctccc cccgggcttc ggccaccgcg ttttcaacaa
      241 accggatcag gtcggcgttg gcggggacgc ccaggggcgg gcgccggcca tacaccgggt
      301 cgaccacaat ttcctccgcc cggcggtcca agacgcccga gagcccacgg acggattcca
      361 ggcgcacgcc ggggaacata tccacggcct ctccgcctcg acggtgggag cgccccaacg
      421 cgtcggagag gtcccaaagg acgcggaccg gggccaaggc cggtctcggg gcccgacgca
      481 agacgaacac cgccacggga cccaggcggc ggtgggtccg gagccgaacc tcgtacacgg
      541 tgccggtcgg gcccatcagt cggacgtgaa ccgtgccgcc gtcgaaagga tcggtgatat
      601 acagtttgtt gttgacgccc acgaccactt cccggtcggc gacggggagc cggttgaagg
      661 tctcgaacaa ccgccggacg tcggcctcgc cgacggattc tcccccggtc aagacggtcg
      721 cgcgccgaag ggccaacacc cagtttctct ccagggcggc ccgggcgtcg gcggcggtcg
      781 ggaatttccc ctcccccacc cgggacatcc gcggccgacc gactcccccc ggccgcgccc
      841 gccggacgcc gccgaaaccg aagaccgata cggggccgcc gtccgccggc tccacgaaca
      901 tcaggagccc cgcgccccag acccattcca acgtcacccg gtgatcggcg ttcggagcca
      961 gggcgtcctt ggggctgtga atttcgacga cgcctcccgg accgtcggga aaatcaaagg
     1021 acaactcgcc cgcggcgttg gtgcgcagga cggcccccaa tccgttaaac gctttccgct
     1081 tttcggcaaa agcgtccggt tcccgaagcg cccacaaaaa tggcggcgcg agggcttcgg
     1141 ccgcgagttc gcgccatcgg tccgccacgg cccgacggcg acgccattcc agtccctttt
     1201 ccgtggtcgg ccgaagatag gccattcgac gtccatcgcg caacacttcc ggcgcgtacc
     1261 aaacggcgtt ccccccggac gcgggcgcga gcgaaaaacg aaccccggcc gttccgtcga
     1321 attcaaaggt cgcccggttg gggcccgggg tcgcccgctc caaaaagagg acttgtttcc
     1381 gggccgccga ttcgccggcg gccgcccggg ggtagcgaag gggaagggtc ccggaggagg
     1441 gaacgttgaa aacgacgggg gcccgctgaa gcgccccgac cgtcgccgcc gcttcctcgg
     1501 cggaaaccga cgccggatcc cgtcgggcga aatcgaacac ggccccggcg aaagccaacc
     1561 ggcggggaag ttcccggtcg gcttccggca cggaagcgaa ggcgtcgaga taatccgcgt
     1621 tgacgcgacc tttcttttcc ggcgcgccgc ggtaacgttc gagaccgtag gccagaaccc
     1681 cctgctcggt ttcgaccagg atcacgggcc cccacccggg cagccaccgg ggacgaaccg
     1741 tttgggcctc cccggacaag gcgtccgttt ggatgacgag ggggcgtccg gagcggggcg
     1801 tggtgaagag cattcggtct tcgcccatcg tgctcaccgg aagaggaacc cccgtttcgg
     1861 aaagcgcatc gatctcccgc aaaaggttcc gatcggggcg atccgcgttc aagagggccc
     1921 ccaaaagagc gttcagcctt tgccgtttgg cctggacggc cagggtgtcg gccaaggtcg
     1981 cgatcgtcga cgcggcctgg ggcgaataca ttcccggggc ccccgccaat acgcgttcgc
     2041 gatggcggtg caggtcgaaa aacagttcgt ccccggccca gaggaccaag cccaaccccg
     2101 cccgccactc caaccggacg ggaaggggct tcccgctcgg caaatggaca aaactgagaa
     2161 ggcggatttt ttggcgcacc aattcgacgt tgccggaccg gggagcccgg agatattccc
     2221 cccgagcgtt ccatcgatcc agggccccca ggaacggggc cggaaggttt tccaaaggca
     2281 tcgcggggtc gaggcgaagg ccctcacgca ccgcccccaa aaggtcgtcc atcgcgtcgg
     2341 ggcgttcgcg caaatcgccg gcggtcgcga ccgtcggatt cggcgccgaa acgggtcgcg
     2401 ccagacgctc cgccgcccgt ccggcggatt ccccgtccag cggttgagcg ggttcccgaa
     2461 acgcccccaa acc
//
LOCUS       DAOQLS010000287         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26242_length_2473_cov_3.447477, whole genome shotgun sequence.
ACCESSION   DAOQLS010000287 DAOQLS010000000
VERSION     DAOQLS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26242_length_2473_cov_3.447477"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="PK362_06165"
     CDS             complement(<1..2027)
                     /locus_tag="PK362_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74451.1"
                     /translation="MTNLSGDLPAGTTTWTQTGLTPNVGRQTLVRAFNNGGTVSSPAS
                     GLFYTAANPPIALSGAAGQTTVALSWGTNGNPAGTLFRYERSLNGVAYAEIGSTVLST
                     ATASGLTPDTTYFFRVRAENASGSPTTYSNVVTTRTIPLAPLAPTLFVIDESTVSIAW
                     GWTDNADNETGYRLRRTTDNASLSGDLPAGTASFLQTGLTPNQSQQVYVEAFNAGGTG
                     ASGSLAHFTDAARPDVPAAAPTVSTVLLTWPANGNPPGTVYESQFGPSVETYGATLAS
                     TATARLFTGLAENTTYFFRVRAVNGDGNPTGYAAVSARTALGMPAAPSTPVVIDRSTG
                     SLKWGWTDNATNEASYRFFARIGASDEDLSGALAPNTTYYTDTNFPTANAPRRAFVRA
                     SNGTGDADSSLSPVAYTLANPPTGSAVTVYVSSVTLRWNANGNAAGTVYRAEKAFGAG
                     AFEEFQSGSVLEAIAVGLATGTYRFRVRAENGEGQLTAYDAVVQASVTVPNVFSVCSD
                     GGAFSYGAANVIVPPGSASCGTVFTVVDPGTFPPGSGPAGTLKPLGVGADISAVPPVC
                     GFSITLSYSDADVAGLDESTLVIARYDGARGFWIPLPSRVDPTTNKVTAAQACTSLVQ
                     VMGRAPSNNVSEGVAYPVPARTQITFSNLPASARIQIYTVSAEKVRELDAD"
     gene            complement(1927..>2473)
                     /locus_tag="PK362_06170"
     CDS             complement(1927..>2473)
                     /locus_tag="PK362_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74452.1"
                     /translation="TPTQAAADHPYVIQQIYNAEALWIAGGDQANYVDFWKDGPVEDA
                     IRYLIHVKKVPVGGLSAGMAIQGDAYFSAMNGTVLSAEALANPYAPEMTIGYGDFLDH
                     PILKDVITDTHYDDPDRRGRHDGDTDLGLDRQRERGERLPGHGRPRSDELVWRPSRRN
                     DDVDPNGSDPERGPANPCSSF"
BASE COUNT          400 a          753 c          901 g          419 t
ORIGIN      
        1 ctgtcggcgt ccaattcccg gactttttcc gcgctcacgg tgtagatttg aatccgggcc
       61 gaggcgggga ggttggaaaa cgtgatttgc gtgcgggccg gcaccgggta agccacccct
      121 tccgagacgt tgttggaggg cgcccgcccc atgacctgga ccaggctcgt gcaggcctgg
      181 gccgcggtga ccttgttggt cgtcgggtcc actcgggagg gcagggggat ccagaagccc
      241 cgggccccgt cgtagcgggc gatgacgagg gtcgactcgt ccaggccggc cacgtccgcg
      301 tcgctgtaag acaaggtaat ggaaaacccg cacaccgggg gaacggccga aatgtcggcc
      361 cccacgccca ggggtttcag ggtcccggcc gggcccgagc cggggggaaa ggttccgggg
      421 tcgaccaccg tgaacaccgt gccgcaggag gccgagcccg gcgggacgat cacgttggcc
      481 gcgccgtagg aaaaggcgcc cccgtcggaa cagacggaga acacgttggg cacggtcacc
      541 gacgcctgaa cgacggcgtc gtaggcggtg agctgtccct ccccgttttc cgcgcgcacg
      601 cggaatcggt aggtgccggt ggccaacccg acggcgatgg cttccaggac cgaccccgac
      661 tggaattcct caaaggcccc cgcgccaaag gctttctcgg cccggtagac ggtgcccgcg
      721 gcgttgccgt tggcgttcca ccggagggtg acgcttgaaa cgtagaccgt gaccgcgctc
      781 ccggtcggcg ggttcgccaa agtgtacgcc acgggcgaga ggctcgaatc ggcgtcgccc
      841 gtgccgttgc tcgcccggac aaaagcccgg cggggcgcgt tggccgtcgg gaaattcgtg
      901 tccgtgtaat aggtcgtgtt cggggcgagg gcgccggaga ggtcctcgtc cgaggcgccg
      961 atccgggcga agaatcggta ggacgcttcg ttcgtggcgt tgtccgtcca cccccatttg
     1021 agggaccccg tcgatcggtc gatcacgacc ggggtcgacg gcgcggccgg catccccagg
     1081 gccgtccggg ccgacacggc ggcatagccc gtggggtttc cgtcgccgtt caccgcccgc
     1141 acccggaaga aatacgtcgt gttttcggcc aaccccgtga acaaccgggc ggtggcggtg
     1201 ctcgccaagg tggccccgta ggtttccacg gacgggccga attgggattc gtagaccgtg
     1261 cccggcgggt tgccgttggc cggccaggtc agcagaacgg tgctgaccgt cggcgcggcc
     1321 gcgggaacgt cgggccgcgc ggcgtcggtg aaatgggcga gggaccccga ggccccggtg
     1381 ccgccggcgt tgaaggcttc cacgtaaacc tgttgggatt ggttgggcgt caaaccggtt
     1441 tgaaggaagg acgcggtccc ggccggaaga tcccccgaca gcgaggcgtt atcggtcgtg
     1501 cgtcgaaggc ggtaaccggt ttcgttgtcg gcgttgtcgg tccatcccca ggcgatcgac
     1561 accgtcgatt cgtcgatcac gaacagcgtc ggggccaagg gcgccagggg gatggtgcgg
     1621 gtcgtcacca cgttggaata agtcgtgggc gatcccgagg cgttttcggc gcgaacgcgg
     1681 aagaaataag tggtgtccgg ggtgagcccg gaagccgtgg cggtcgagag gaccgtggac
     1741 ccgatttcgg cgtaggccac gccgttcaag gaacgttcgt agcggaacag cgtgcccgcc
     1801 gggtttccgt tggtccccca ggacaaggcg accgtggttt ggccggccgc gcccgacaag
     1861 gcgatgggcg ggttggccgc ggtgtagaaa agccccgagg ccggcgatga caccgtgccg
     1921 ccgttgttaa aagctcgaac aagggtttgc cggcccacgt tcggggtcag acccgtttgg
     1981 gtccacgtcg tcgttccggc gggaaggtcg ccagacaagt tcgtcactcc gggggcgtcc
     2041 atgacccggt agccgttctc cccgctcacg ctgtcgatcc aggcccaggt cagtgtcccc
     2101 gtcgtggcgg ccacggcggt cgggatcgtc gtagtgggtg tcggtaatga cgtctttaag
     2161 gatggggtga tccagaaaat cgccgtagcc gatggtcatt tcaggggcat acgggttggc
     2221 cagcgcctcg gcggagagga cggtgccgtt catcgcggaa aaataggcgt cgccctgaat
     2281 ggccatgccg gcgctaagcc cgccgaccgg cacttttttg acgtgaatca ggtagcgaat
     2341 ggcgtcttcc accgggccat ctttccagaa atcgacgtag ttggcctggt cgcccccggc
     2401 aatccagagg gcttcggcgt tgtatatctg ctggatcacg tagggatggt cggcagcggc
     2461 ctgggtgggc gtg
//
LOCUS       DAOQLS010000288         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26265_length_2472_cov_4.527100, whole genome shotgun sequence.
ACCESSION   DAOQLS010000288 DAOQLS010000000
VERSION     DAOQLS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26265_length_2472_cov_4.527100"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK362_06175"
     CDS             complement(<1..298)
                     /locus_tag="PK362_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNC74453.1"
                     /translation="MNLLWLSDDDVAQLVTVGEALPLVEEAFRALAEGRAQMPAKMYL
                     DFKEFGGDLRAMPAYLPKTGTDGRGFAGVKVVNSHPGNPARGLPTVSAVLGLDDP"
     gene            complement(295..1584)
                     /gene="hemL"
                     /locus_tag="PK362_06180"
     CDS             complement(295..1584)
                     /gene="hemL"
                     /locus_tag="PK362_06180"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNC74454.1"
                     /translation="MSDARPRSEALFAEAKQRLVGGVNSPVRAFGAVGGTPVFIERAR
                     GPRLWDVDGNAYLDYVGSWGPMILGHAHPAVVRAAQRAVELGSSFGAPTAAEVTLAQL
                     VREAFPTAERVRFTSSGTEACLTALRLARGVTGRPLIVKFAGCYHGHGDSLLVSAGSG
                     ALTTGRPNSAGVPAELARLTLVLPYNDPDALAAAFAKHGKKIAAVIVEPVVGNMGVVG
                     PRPDFLDALERLPRRHGALLILDEVMTGFRLSWGGAQRLLALRADLTTFGKIIGGGFP
                     VGGLGGPRRLMDQLAPLGPVYQAGTLSGNPVAMAAGAATLGLLKKDPPYKFLRDTTDH
                     LVHGLRAVARRRRREVTVNAVESMFTVFFAKGPVTDLKSAQRANAKLYARFFHGLLRR
                     GVYFPPSQFEAAFLSAAHTPADIEKTVAAADAAFRDL"
     gene            complement(1586..2008)
                     /locus_tag="PK362_06185"
     CDS             complement(1586..2008)
                     /locus_tag="PK362_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74455.1"
                     /translation="MKTRRSLWGVLTIALAVAVGAGAAEKKWTLSEWLKDLDSKIRRV
                     QDKHKNQKVAVAAVRGDKAKPTTKLYWKGEKGEVSDAEIADFKAAVDLAQTGKPDEAR
                     AALDAFLAKYPATAMKADVEQTRSLLTAAPVESAAPAQ"
     gene            complement(2035..>2472)
                     /locus_tag="PK362_06190"
     CDS             complement(2035..>2472)
                     /locus_tag="PK362_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74456.1"
                     /translation="VKDELAKALDVYLESAAAPLAAALADNPKDTAALLELGLLYAEN
                     ERWEKSAEAFDRLLALEPDNAAALNDRANLYLIAKDAAKARELYEKAAAADAKDSGIQ
                     LNIVRAAKTAGDKTAAKAAFNKALEIAPELKKTYTTWEEASEW"
BASE COUNT          391 a          852 c          811 g          418 t
ORIGIN      
        1 cggggtcgtc cagccccagc accgccgaca ccgtggggag cccccgggcg ggattgcccg
       61 gatgcgaatt gaccaccttg accccggcga acccccggcc gtccgttccc gtcttcggca
      121 gataggccgg catggcccgc aagtcgccgc cgaattcctt gaagtccaaa tacatcttgg
      181 ccggcatctg ggcccggcct tcggccaggg cgcgaaaggc ctcctccacc aggggcaggg
      241 cctcgcccac cgtgaccagt tgggccacgt cgtcgtcgga caaccacaac agattcacag
      301 gtcgcggaag gcggcgtcgg ccgcggccac ggttttttca atgtccgcgg gcgtgtgggc
      361 ggccgacagg aaggcggcct cgaactggga cggcgggaaa tagaccccgc ggcgaagcaa
      421 tccgtggaag aaccgggcgt agagtttcgc gttggcccgt tgggcgctct tgaggtccgt
      481 cacgggcccc ttggcgaaga aaacggtgaa catcgactcc acggcgttca cggtcacctc
      541 ccgccggcgg cggcgggcca ccgcccgaag gccgtggacc aaatggtccg tggtgtcgcg
      601 aaggaactta tacggcggat cttttttaag aaggccgagg gtggccgcgc cggccgccat
      661 ggccacggga ttgccggaga gggttccggc ttggtacacg gggcccaggg gcgccaactg
      721 atccatcaag cggcgcggcc cgcccaaacc gcccacgggg aatcccccgc cgatgatttt
      781 gccgaacgtt gtcaaatcgg cccgcagggc cagcagccgc tgggccccgc cccaggacag
      841 gcgaaagccg gtcatcactt cgtccaaaat cagcagggcg ccgtgccggc ggggcaggcg
      901 ttccagcgcg tccaaaaagt ccggccgggg ccccacgacc cccatgttgc cgaccacggg
      961 ttccacgatg acggcggcga tttttttgcc gtgcttggcg aaggccgcgg ccagggcgtc
     1021 ggggtcgttg tagggaagca cgagggtgag ccgggccaac tccgccggca cgcccgccga
     1081 attgggccgc ccggtcgtca gcgcgccgct ccccgccgac accagcaggc tgtcgccgtg
     1141 cccgtgataa caccccgcga atttgacgat caacggccgg ccggtgactc cccgggccaa
     1201 ccgcagggcc gtgaggcagg cttccgtgcc cgaactcgtg aaacggaccc gttcggcggt
     1261 gggaaaagcc tcccggacca actgggccag ggtcacctcc gccgcggtgg gcgccccgaa
     1321 agaactgccg agctccacgg cccgctgggc ggcccgaacc accgccgggt gggcatggcc
     1381 caggatcatg ggcccccagg aacccacgta atccaagtag gcgttcccgt ccacgtccca
     1441 caaacggggg ccccgggcgc gctcgatgaa cacgggcgtg ccgcccacgg cgccgaaggc
     1501 gcgcaccggg gaattgacgc cgccgaccag gcgttgcttg gcctcggcga acagggcttc
     1561 cgagcggggg cgggcgtcgc tcacgttatt gggcgggggc cgcgctttcc accggcgccg
     1621 ccgtcagcag agaccgggtt tgctccacgt cggccttcat cgccgtcgcc ggatacttgg
     1681 ccaggaacgc gtccaaagcc gcccgggcct catcgggttt ccccgtctgg gccaaatcca
     1741 ccgcggcttt gaaatccgcg atttcggcgt cggagacctc gcccttttcg cctttccagt
     1801 aaagcttcgt ggtcggcttg gccttgtccc cgcggacggc ggccacggcg actttttgat
     1861 ttttgtgctt gtcctggacg cgccggattt tggaatccaa gtccttgagc cactcggaca
     1921 acgtccactt cttttccgcc gcgccggcgc ccaccgccac cgccaacgcg atcgtcaaaa
     1981 cgccccacag cgaacgacgt gttttcatgg ttcctccttg attcccgaag acgatcacca
     2041 ctccgaggct tcttcccagg tggtgtaggt ttttttgagc tcgggcgcga tttccaacgc
     2101 cttgttgaag gccgccttgg cggccgtctt gtcccccgcg gttttggccg cgcggacgat
     2161 gttcaattga atgcccgaat ccttggcgtc ggccgccgcg gccttttcgt aaagttcccg
     2221 ggccttggcc gcgtctttgg cgatcaggta gaggttggcg cgatcgttca gcgccgccgc
     2281 gttgtccggc tccagcgcga gcaggcggtc gaaggcctcg gcgctttttt cccaccgctc
     2341 gttttcggcg tagaggagac ccaattccag cagggcggcc gtgtccttgg gattatccgc
     2401 caaggccgcg gcgagggggg cggccgcgct ttccaggtaa acgtccagcg ccttggccaa
     2461 ttcgtctttg ac
//
LOCUS       DAOQLS010000289         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26460_length_2461_cov_5.716542, whole genome shotgun sequence.
ACCESSION   DAOQLS010000289 DAOQLS010000000
VERSION     DAOQLS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26460_length_2461_cov_5.716542"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PK362_06195"
     CDS             <1..444
                     /locus_tag="PK362_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74457.1"
                     /translation="AAAGLGDPGLSTNFLAARVRRLSGEPDTRFAALWTVAGEPVAVF
                     GPAPCPRAPASWTGGASDRLEEAPGNPASAVFIAPLWRGGKKAGVLTWGRWPPAAGSA
                     RAADDRSLAAAFLWWAAAGALGIFGVGALGNRDPAGRGPPGQSPV"
     gene            534..2351
                     /locus_tag="PK362_06200"
     CDS             534..2351
                     /locus_tag="PK362_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC74458.1"
                     /translation="MATRGNIGRKQAKEILIEARLVNADGLAAAEKETLKSDRPLQQI
                     VVDLKLADKVDVLQALSREWRTKAVDLAEMEIDPEVVRILPESTARKSLVLPFAKEDQ
                     VLLVAMADPRDFLVSEDIHLRTGLEVQRYLAMPEDILKELDKVYGVGAAGKAEELLKS
                     VTDSAIPEGEGLERIQEKSDVTEVDASAPEVEKIVNAVILSALQMKASDIHIEPFEDP
                     AGKHSKILVRFRVDGFLRMANFQVPWMYRAAVMAKIKIMTNTMNITERRIPQSGRIQV
                     MAKGNPIEFRVEIIPTVYGESCVMRILDRKAVQVDIQRLGFHQETLDKFLGLLKGVGG
                     KKNFGIVVVCGPTGSGKSTTLYAALNHINRPDIKILTAENPVEYNLDGIVQVQVNPDL
                     HMGEDKCFDFATALRSFLRLDPDVIMVGEIRDQETAHIAMEAAMTGHLVFSTIHTNDA
                     PSTVARLVDMGIPSFMVASTVKCVLAQRLCRRVCADCKTARAPTPDEVEVFKTANFPL
                     PANAQLYYGKGCSTCNDSGFKGRCGIHELLVVDDPVRAAVLKDVNADTVREAATVVSP
                     QKMRTIIADGLQKALDGMTTVREVLGGASEEIEGKDKKK"
BASE COUNT          473 a          783 c          781 g          424 t
ORIGIN      
        1 gcggcggccg ggttgggcga tcccggcctt tcgacaaatt ttctcgcggc gcgggttcgc
       61 cgtttgtcgg gcgagccgga cacccgattc gccgctcttt ggacggtcgc cggggaaccc
      121 gtcgcggttt tcggccccgc gccatgtccc cgcgcgcccg cgtcctggac cggcggcgcc
      181 tcggaccggc tggaggaagc ccccgggaat cccgcgagcg ccgtttttat cgcgcccctg
      241 tggcgcgggg ggaaaaaggc cggcgtgctc acctggggcc ggtggccgcc cgcggcggga
      301 tccgcccggg cggcggacga ccggtcgttg gcggcggctt ttctctggtg ggcggcggcg
      361 ggcgctctgg ggattttcgg cgtgggcgcc ctgggaaacc gcgacccggc cgggcgtggc
      421 ccccctggac aatcccccgt ttgagggcta aataaggaag caggaccttc gccgctcggc
      481 gaatcgcgcg tctcggcgct cgcgcccggg gcgacaacag gaggtagcgc ttcatggcca
      541 cccgtggcaa catcggccga aaacaggcca aagaaatttt gatcgaagcc cgcttggtca
      601 acgccgatgg cctggcggcg gcggaaaaag aaaccctcaa gtcggaccga ccccttcaac
      661 aaatcgtcgt cgatctgaag ctggccgaca aagtggacgt gctccaggcc ctgtcccggg
      721 agtggcgcac caaggccgtc gacctggccg agatggaaat cgatcccgaa gtggtgcgga
      781 tccttccgga atccaccgcc cgcaaaagtt tggtcctccc cttcgccaag gaagatcagg
      841 tcctcctggt ggccatggcc gatcctcggg atttcctcgt gtccgaagac atccacctgc
      901 gaacgggcct ggaggtgcag cgctacctcg ccatgcccga ggacatcctc aaggagttgg
      961 acaaagtcta cggggtcggc gcggcgggca aggccgagga acttctcaaa tcggtcacgg
     1021 actccgccat tcccgaaggc gaggggctgg agcggattca ggagaagtcc gacgtcaccg
     1081 aagtcgacgc ctcggcgccg gaagtcgaaa agatcgtcaa cgccgtcatc ctgtcggccc
     1141 ttcaaatgaa ggcctccgac attcacatcg agccttttga agaccccgcc gggaagcatt
     1201 ccaaaatcct cgtgcggttc cgcgtggacg gttttctccg catggcgaac ttccaggtgc
     1261 cctggatgta ccgcgccgcc gtcatggcca aaatcaaaat catgacgaac accatgaaca
     1321 tcaccgagcg gcgcattccc cagtcgggcc gcatccaggt catggccaag ggcaacccca
     1381 tcgaatttcg cgtggaaatc atccccaccg tgtacgggga atcctgcgtc atgcgtatcc
     1441 tggaccggaa agccgtccag gtcgacattc agcggctcgg gtttcatcag gagaccttgg
     1501 acaagtttct cggccttctc aaaggcgtcg gcggaaaaaa gaacttcggc atcgtggtcg
     1561 tgtgcggtcc cacggggtcc ggaaaatcga ccaccctcta cgcggcgtta aaccacatca
     1621 accggcccga cattaaaatt ctcacggcgg aaaaccccgt cgaatacaac ctggacggga
     1681 tcgtgcaggt tcaggtgaac cccgacctgc acatgggcga ggacaaatgc ttcgattttg
     1741 cgacggccct gcggtccttc ctgcggttgg accccgacgt catcatggtc ggtgaaatcc
     1801 gggaccagga aacggcccac atcgccatgg aagccgccat gaccggccac ttggtgttct
     1861 ccaccattca caccaacgac gcgccgtcca cggtggcccg gttggtggac atgggcatcc
     1921 cctccttcat ggtggccagc acggtgaagt gcgtgttggc ccagcggttg tgccgacgcg
     1981 tctgcgccga ttgcaaaacc gcccgggccc cgacgccgga cgaagtcgaa gtctttaaaa
     2041 ccgcgaactt tccgctgccc gccaacgccc agctttacta cgggaaaggg tgttccacct
     2101 gcaacgattc cgggttcaaa ggtcgttgcg gtattcacga gttgttggtg gtggacgatc
     2161 cggtccgcgc cgcggtgctc aaggacgtca acgcggacac ggtgcgggag gcggccacgg
     2221 tggtctcgcc ccagaaaatg cgtaccatca tcgccgacgg actgcaaaaa gccctggacg
     2281 ggatgaccac ggtccgggaa gtcctgggcg gcgccagcga agaaatcgaa ggaaaagaca
     2341 agaaaaagta attccgcctt tttcaacgcc cgcgggggtg tttccgctca gaacggaaac
     2401 ccggccgcgg gcgttttggt ttttggggcg gcccggccgg gacgggccgg ttcaatcggg
     2461 g
//
LOCUS       DAOQLS010000290         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26592_length_2454_cov_4.032514, whole genome shotgun sequence.
ACCESSION   DAOQLS010000290 DAOQLS010000000
VERSION     DAOQLS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26592_length_2454_cov_4.032514"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK362_06205"
     CDS             <1..546
                     /locus_tag="PK362_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNC74459.1"
                     /translation="TKTFMNTRPRAWHRLMEKIAAVVTDLLNAQIDAGVQGVQLFDSW
                     VGVLSPSDYKTFVLPHSRSVFRGVRRGVPTIHFGTQTGTLLELLKEAGGSVIGIDWRV
                     TFREARRRLGDVPLMGNLDPALLFSPPAAIDREVRRWLSDAGRRPGFIANLGHGILPG
                     TPFRNVVHLVKTVKRLGRRRP"
     gene            543..>2454
                     /gene="hemG"
                     /locus_tag="PK362_06210"
     CDS             543..>2454
                     /gene="hemG"
                     /locus_tag="PK362_06210"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNC74460.1"
                     /translation="MKPGVLLVGFGGPTRAEDIRPFLSNVLRGRPVPPHRVDEVVKQY
                     ERIGGRSPYNELVGDLSRALSARLQSDGVDLPVYTGMRNWSPTLAAALKNAADDGVTD
                     LVAVPLAAFRSIPSWNYYLKSTAEAHRAARRRPMRLHFVQPWHAHPAFRDAVADRVAE
                     ALRAAPNPPGNDRWWFTAHSIPTPWDAASGYSGQLKALAEAVAARFGVSDFRLVYQSR
                     SGRPEDPWLDPDISDALARNPGAGRRAVVIPIGFLMDHVEVLFDLDVRARETGHAHGW
                     DYLRAKTVGDHPRFVALLSALVQERLRIAANRPAGPGPSRRFVVVGGGAAGLSAAHRA
                     VELSARRGEPLDLAVLESRPRAGGVIETGRSDGFLWESGPDSFITEKPALLNLAERLG
                     LSGQIIGTNPSFQKSFVALRGRLHSTPEGFYLLAPSRLRPLIRTPLLTLWGKFRAALE
                     LVIPPRRSPGDESLASFVRRRFGREVLERLAQPMVAGIYSADPEKLSLRATFPRFLEM
                     EARGGVIRSLLRARWKKSAGKGAVPARGTSGPRYGLFATFRDGLDTFVDALRAELPPT
                     ALRTGAEVRSVDKIGGGWRLTLASGERLETPAVCLAVSAPVAARLLRPVDPVLATLLE
                     GIPFGDVATVNLA"
BASE COUNT          357 a          873 c          773 g          451 t
ORIGIN      
        1 accaaaacat tcatgaacac ccggccccgg gcctggcacc gattgatgga aaaaattgcc
       61 gcggtggtga ccgacctact caacgcccaa atcgacgccg gcgttcaggg cgtccaactt
      121 tttgacagtt gggtgggggt gctttccccc agcgactaca aaaccttcgt tttgccccac
      181 agccgctccg tgtttcgcgg cgtgcgccgg ggcgtgccga ccattcattt tgggacgcaa
      241 acggggaccc ttctggaact cctgaaagaa gcggggggga gcgtcatcgg catcgattgg
      301 cgcgtcacgt tccgggaggc ccggcgccgg ctgggcgacg tcccgctcat gggcaacctc
      361 gatccggccc ttctcttttc gccccccgcc gccatcgatc gggaagtccg acggtggctg
      421 tccgacgccg ggcgacgccc gggcttcatc gcgaacctcg gccacggcat attgcccggc
      481 accccttttc ggaacgtcgt tcacctcgtc aagacggtca aacgcctcgg ccgccggcgg
      541 ccatgaaacc cggcgttttg ctggtcgggt tcggcggccc cacccgcgcg gaagacattc
      601 gcccctttct ctccaacgtc ctccggggcc gtcccgttcc accccaccgg gtggacgaag
      661 tggtcaaaca atacgaacgc atcggcgggc ggtcgcccta taacgagttg gtgggcgatt
      721 tgtcccgcgc cctgtcggcg cgcctgcaat cggacggcgt cgatctcccg gtttacacgg
      781 gcatgcgcaa ctggtccccg accctggccg ccgcgttgaa aaacgccgcg gacgacggcg
      841 tcaccgactt ggtggcggtt cccttggccg ctttccggtc cattcccagc tggaactatt
      901 atttgaaatc cacggcggag gcccaccgcg ccgcccggcg gaggccgatg cgacttcatt
      961 tcgtccagcc gtggcatgcc cacccggcgt tccgggacgc ggtggccgac cgggtggccg
     1021 aagcccttcg cgccgcccca aacccccccg gaaacgatcg gtggtggttc accgcccatt
     1081 ccatccccac cccctgggac gccgcctcgg ggtattccgg ccaattgaag gccttggcgg
     1141 aggccgtcgc cgcccgattt ggcgtgtccg attttcgact ggtgtaccaa agccgcagcg
     1201 ggcgcccgga agatccctgg ctcgatcccg acatttccga tgccctcgcc cggaaccccg
     1261 gcgctgggcg tcgggcggtg gttatcccca tcggattttt gatggaccac gtcgaagtgc
     1321 tttttgattt ggatgttcgg gcccgggaaa ccggacacgc ccacgggtgg gactaccttc
     1381 gcgcgaaaac cgtcggggac cacccgcgct tcgtcgcctt gctatccgcg ctggttcaag
     1441 aacgcctgcg aatcgccgcg aatcgccccg ccgggcccgg accgtcccga cgtttcgtgg
     1501 tggtcggcgg aggcgccgcg ggattgtccg cggcgcaccg cgccgtcgaa ctttccgcgc
     1561 gccgcggcga gcccctggac ctggccgtcc tcgaatcgcg cccccgggcg gggggcgtca
     1621 tcgaaacggg ccggagcgac gggtttttgt gggagagcgg gcccgacagt tttatcaccg
     1681 agaaacccgc cctgttgaat ctggcggaac gcctgggctt gtcgggccaa atcatcggca
     1741 ccaacccgtc ctttcaaaaa agtttcgtcg ctctccgcgg acgcctgcat tccacgccgg
     1801 agggtttcta tttgctggcc ccctcccgcc ttcggccgtt gatccgtacg ccgttgctca
     1861 cgctgtgggg aaaattccgc gcggcgctgg aactcgtgat cccgccgcgc cgttcccccg
     1921 gggacgaatc cctcgcctct ttcgtgcgtc ggcgtttcgg ccgggaagtc ctcgaacggt
     1981 tggcgcaacc catggtcgcc ggcatctact ccgccgatcc tgaaaaactg agccttcgag
     2041 ccacctttcc ccggtttctg gagatggagg ctcggggcgg agtgattcgc tcccttctgc
     2101 gcgcccggtg gaaaaaatcc gcggggaaag gcgccgtgcc cgcccggggg acgagcggcc
     2161 cccgttacgg gcttttcgcc acttttcggg acggcctcga tacgttcgtg gacgcccttc
     2221 gcgccgagct accgcccacc gccctccgca ccggcgccga ggtccggtcc gtcgacaaaa
     2281 tcgggggggg gtggcgcctg acgctcgcct ccggggaacg cctcgagacc ccggcggtgt
     2341 gcctcgccgt gtcggcgccg gtggccgcgc gactgcttcg cccggtggat ccggtcttgg
     2401 cgacgctcct cgagggcatt cccttcggcg acgtggccac cgtcaacctc gcct
//
LOCUS       DAOQLS010000291         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26906_length_2439_cov_3.821728, whole genome shotgun sequence.
ACCESSION   DAOQLS010000291 DAOQLS010000000
VERSION     DAOQLS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26906_length_2439_cov_3.821728"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2439
                     /locus_tag="PK362_06215"
     CDS             <1..>2439
                     /locus_tag="PK362_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74461.1"
                     /translation="DNPPLTRTLIVEDAVVPLANFTGQFDRLKPGDDYTVDYRRGLIQ
                     FKRRVDDNAVLAVDFTFTDGTKLSDLNTPGRLKLIKTAGDQPITSGPIDPAETGYRRE
                     LKTFYSLGNIKIVRDNGRGNFILRTVDANQNDKVVLISTAADASVDAGTLTYPRSLEV
                     DFDLGVFNVALSSVVFDAQLYGPSPTSNFSFLSEYRYRLKDYQVRPNIVFGSERVTVN
                     GRPMKNEIDYFMDYGTGLLQFFNEDELDESSQIEVTYEYAPFGGQLGQTLVGTRAELD
                     IVPNRFKVGSTFLYTFAPKTDLIPDVRSTPNSLMVLEMDGRVTDVEVPFVPLKFTLAG
                     EAAQSRENPNLFGKALVDSMEGIRQEDGAVLDLDFWQLASPPSLLNGGVYGEATRPTA
                     VAVGDELFKLRDIVNNSVDIPENESLRVLRVDYSLLRKPGGGPADAEEASLIQPISKA
                     GRDFSKKAYIDLWVEGAGAAGANVDLDVDAGSLNEDADLDNNRDREDLNDDGTLNSGE
                     DIGFPYDFQSTTFRQGAGNGRLDTEDLDGDGVLQATDRKPFAGPLFRLSNGIAAVDRN
                     GVSSTINDLGFTGWRFIRIPLNTSLGDFHSIRQVRLTLRGLESAPGVARAGTVRFGKI
                     AFVGNTWESATVTAGAAMDVAAVNNLDNPDYRSLLGDPSYNEIYGDQASNRTREQALQ
                     LSFNLPAGSTATTRSVYGLARDFSHHRTLNFFYQVPLGQPTDETLLLQMGSETDYFQW
                     ALPLSAAHVNRWTLESLRLVDVNNDGTPDLPQSDTPGARVTIVGVPSLARIGQLKIGV
                     TNGTGGAIAGKVWVN"
BASE COUNT          461 a          795 c          764 g          419 t
ORIGIN      
        1 gacaacccgc ccctgacccg gacgctgatc gtggaagacg ccgtcgtgcc cctggccaat
       61 tttacgggcc aattcgaccg gttgaaaccc ggggacgatt acaccgtgga ctaccgccgg
      121 gggctcatcc aattcaagcg ccgggtggac gacaacgcgg tcctggccgt ggacttcacc
      181 tttaccgacg gcaccaaact ctcggacctg aacaccccgg gccgcctgaa gctcatcaaa
      241 accgccgggg accagccgat cacgagcggc cccatcgacc cggccgaaac gggctaccgg
      301 cgggagctta aaacctttta ctccctgggc aacatcaaaa tcgtccggga caacggccgg
      361 ggcaatttca ttctccgcac cgtggacgcc aatcaaaacg acaaggtcgt cctgatcagc
      421 acggccgccg acgcgtcggt ggacgcgggc acgctcacct acccccgcag tttggaggtg
      481 gatttcgatt tgggcgtctt caacgtggcg ctgtcctcgg tggtgttcga cgcccagttg
      541 tacgggccct ccccgacgtc caatttcagc ttcctttcgg agtaccgcta ccgcctgaag
      601 gactaccagg tgcggcccaa catcgtcttc ggcagcgagc gggtcacggt gaacgggcgg
      661 cccatgaaga acgaaatcga ttatttcatg gactacggca cgggccttct gcagttcttc
      721 aacgaggacg agctcgacga atccagccag atcgaagtca cctacgaata cgcccccttc
      781 ggcggccagt tggggcagac gctcgtgggc acccgggccg aactggacat cgtgccgaac
      841 cggttcaagg tgggctcgac ctttctctac accttcgccc ccaagacgga tctgatcccc
      901 gacgtgcgct cgacccccaa cagcctcatg gtgctcgaaa tggacggacg ggtgacggac
      961 gtcgaagtgc ccttcgtgcc gctcaaattc accctggcgg gggaggcggc ccagtcccgg
     1021 gaaaacccga acctgttcgg caaagccctg gtggattcca tggagggcat tcgccaggag
     1081 gacggcgccg tgctcgattt ggatttttgg caattggcct cgccgccgtc cctcctgaac
     1141 gggggcgtct acggcgaagc cacgcggccg acggcggtgg cggtggggga cgaactgttt
     1201 aaactgcggg acatcgtcaa caattccgtg gacattccgg aaaacgaaag ccttcgggtc
     1261 cttcgggtcg actacagcct gctgcggaag cccggcggcg ggccggccga cgcggaggag
     1321 gcgtccctga tccaacccat ctccaaagcc gggcgggatt tctccaagaa ggcctacatc
     1381 gatttgtggg tggagggggc cggggccgcc ggcgccaacg tcgatctgga cgtggacgcc
     1441 gggtcgttga acgaagacgc cgatttggac aacaatcggg accgggagga tttgaacgac
     1501 gacggtacct tgaacagcgg ggaagacatc ggcttcccct acgatttcca atcgacgacc
     1561 ttccgtcagg gcgcggggaa cgggcggctg gacaccgagg acctggacgg ggacggggtc
     1621 cttcaggcga cggaccgcaa gcccttcgcg ggccctctct tccgcctgtc caacggaatc
     1681 gcggccgtgg accgcaacgg cgtgagctcg accatcaacg acctggggtt cacggggtgg
     1741 cgatttattc gaatcccgtt gaacacgtcc ctgggggact ttcattccat ccgccaggtg
     1801 cgcctcactc ttcgggggtt ggaatcggct cccggcgtgg cccgggcggg gacggtgcgg
     1861 ttcggcaaaa tcgccttcgt ggggaacacc tgggaatcgg ccacggtgac cgccggggcc
     1921 gccatggacg tggccgcggt gaacaatttg gacaacccgg attaccgctc cctcctcggc
     1981 gacccgtcct acaacgaaat ctacggcgac caggcctcca accgcacccg ggagcaggcg
     2041 ttgcagcttt cgttcaatct gcccgccggt tcgaccgcca cgacccggtc cgtgtacggc
     2101 ttggcccggg atttttccca ccaccggacc ctgaatttct tttaccaagt ccccctgggc
     2161 cagccgacgg acgaaaccct gttgcttcag atgggttcgg aaacggacta tttccaatgg
     2221 gctctgccgt tgtccgccgc ccacgtgaac cggtggaccc tggaaagcct ccgcctggtg
     2281 gacgtcaaca acgacggcac gcccgatctg ccccagtcgg acaccccggg ggcccgggtg
     2341 accatcgtgg gcgtgccgtc cctggcgcgg atcggccaat tgaaaatcgg cgtgacgaac
     2401 ggcaccggcg gggcgatcgc gggcaaagtc tgggtgaac
//
LOCUS       DAOQLS010000292         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_26952_length_2437_cov_3.077666, whole genome shotgun sequence.
ACCESSION   DAOQLS010000292 DAOQLS010000000
VERSION     DAOQLS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26952_length_2437_cov_3.077666"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..>2437)
                     /locus_tag="PK362_06220"
     CDS             complement(65..>2437)
                     /locus_tag="PK362_06220"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNC74462.1"
                     /translation="PRLDPNTGQFTIARLIPDASYDVTFRGFSEFGRESQAKGLRTYA
                     PVVLGGVRVAPGEIVDLGTVDLSQGGTLAGTVTDTDGAPLANIRLTAVPALTNGEDRH
                     NFEIETFTDEQGHYEFHGVDRNQRYYDVIASPRFRSGDVFGQLAGTKYAEERVRMIDV
                     NDPLKITGIDFALTAASGALIGTIRTVDGGTLVPAFENGNNQAGVRGANIVLHFDGAV
                     LDDNPLGEIQETTSPDGTFRIDGLKPGAYTFRGIASGYSTALKKIVVAKTGTTNAGTV
                     TLGRGATVSGAIAKPDGSAPSLSELQMLVGVDRNFEDFVFGTMEANSDTQLVTGYSLS
                     GFQPGKNYSLIFVSKKGEVGEAATDVVFSTTTESKVIDVVFRPAGPKVFVNQERAGNQ
                     YTLRFFTSQPLRNLTDADNDTNQIVTISSGAGTIASVDLSSSRDFLTVVYDAAPIETS
                     FVLHLNFHTTIVDPDDPAGDTFLFDQDFEFFAGIGRRRSASIPNVTGGDCSLEGVATG
                     ASFPSGTFAVDRSSSVEVGLQSAVSLDQLPAGAPPLSGRAARAARFAAETAERLGPAA
                     YPVAGLYRAVRLAPTVSPFSAFYDIFLPAGVSHVLKNEALLTLKYDDSITDPSALNVY
                     YFDPNNNVFLLEKSQRTVDTVNKTITVGVRHLSTFVVLRGQAPVVGTNNYSGQKLFVH
                     NVPNPFNLESKTVTLNHTTPAQDQVTEGTVIAYGLPAGTAGSVKIEIYDVAGGLVRTL
                     VQSAPSGGTYYYTDWDGKNDAGDKVASGVYIGRMTVNGGDEAFFKMAGVK"
BASE COUNT          445 a          735 c          789 g          468 t
ORIGIN      
        1 tggacgaacc ccgccgacgc caaaaggccc cccgtcgcca tcgcaaaaag ccgcgttcgc
       61 cgaatcattt gacccccgcc atcttgaaga aggcttcgtc cccgccgttc accgtcatcc
      121 ggccgatgta gaccccggag gccactttgt cgccggcgtc gtttttgccg tcccaatcgg
      181 tgtaatagta cgttccaccg ctgggggcgg actgaacgag ggtccgcacc aaccccccgg
      241 ccacatcgta aatctcgatc ttcacggagc cggccgttcc cgccggcaac ccgtaggcga
      301 tcaccgtgcc ttcggtgacc tggtcctggg cgggcgtcgt gtggttcaac gtgaccgttt
      361 tggattccaa attaaaagga ttcgggacgt tgtggacgaa cagtttttga cccgaataat
      421 tgttggtccc caccacgggc gcctgacccc gcaggaccac gaaggtggac aggtgccgga
      481 ccccgacggt gatggttttg ttgacggtgt ccacggtccg ctggcttttt tccaacagga
      541 acacgttgtt gttggggtcg aaataataca cgttaagcgc cgacgggtcg gtgatggagt
      601 cgtcgtattt cagcgtcaac agggcctcgt tttttagaac gtggctgacg ccggccggca
      661 ggaaaatgtc gtaaaaggcg ctgaaagggg acaccgtggg ggccaagcgg accgcccggt
      721 agaggccggc cacgggatag gccgcgggtc ccaggcgttc cgccgtctcg gccgcgaagc
      781 gggcggcccg ggccgcccgg cccgacaggg gcggagcgcc cgcgggcaat tgatcgaggc
      841 tcaccgcgct ttgcaaaccg acctccacgg aggaggatcg atccacggcg aaggtcccgg
      901 aggggaacga cgcgcccgtg gcgacgccct ccagggaaca gtcgccgccc gtcacgttgg
      961 gaatcgaggc ggaccgacgc cggccgatgc cggcaaaaaa ctcaaagtct tggtcgaaca
     1021 gaaaggtgtc gcccgccgga tcgtccgggt cgacaatcgt cgtgtggaaa ttcaagtgga
     1081 ggacaaagga ggtttcgatg ggcgcggcgt cgtaaaccac cgtcaggaag tcccgcgagg
     1141 aacttaaatc cacgctggcg atggtgcccg ccccgctgga aatcgtgacg atttgattgg
     1201 tgtcgttgtc cgcgtcggtc aagttgcgaa ggggctggct ggtgaagaag cgcagggtat
     1261 actggttgcc cgcccgttcc tggttgacga acaccttggg ccccgccggc cggaagacca
     1321 cgtcgatgac tttgctttcg gtggtggttg aaaaaacgac gtcggtcgcg gcttccccca
     1381 cctcgccttt tttggaaaca aaaatcagcg aatagttttt gcccggttgg aatccggaca
     1441 gggagtagcc cgtgaccaat tgggtgtcgg aattggcctc catggtgccg aacacaaaat
     1501 cctcgaagtt gcggtccacg cccaccagca tttgcagttc ggaaaggctg ggagcggaac
     1561 cgtccggttt ggcgatggcc ccggacaccg tggcgccccg gcccagggtg acggttcccg
     1621 cgttggtcgt gccggttttg gcgaccacga ttttcttcaa ggccgtgctg taacccgacg
     1681 cgatgccccg gaaggtgtag gcgccgggct tcaacccatc gatgcggaag gtgccgtccg
     1741 gcgaggtcgt ttcctggatt tcccccaggg ggttgtcgtc cagaaccgcc ccgtcgaagt
     1801 gcaggacgat gttcgcgccg cgaaccccgg cctggttgtt cccgttttca aaggcgggaa
     1861 caagcgtccc gccgtccacg gttcgaatgg ttccgatcaa ggccccgctg gcggcggtca
     1921 aggcgaaatc gatgcccgta attttgaggg ggtcgttcac gtcgatcatt cgaacccgtt
     1981 cttcggcgta tttggttccg gccaactggc caaagacgtc cccggaacgg aaacgggggg
     2041 acgcgatcac atcgtagtac cgttggttcc ggtccacgcc gtgaaattcg tagtgccctt
     2101 gttcgtccgt gaacgtttcg atttcaaaat tgtggcggtc ttcgccgttc gtgagggccg
     2161 gaacggcggt gagccggatg ttggccagcg gcgccccgtc ggtgtcggtc accgtccccg
     2221 ccagggttcc cccctggctc aagtccacgg ttcccagatc gacgatttcc ccgggggcca
     2281 cgcggacccc gcccaggacc acgggcgcgt aggttcgaag tcctttggct tgggattccc
     2341 ggccgaattc cgaaaacccg cggaacgtca cgtcgtacga cgcgtccgga atcaggcggg
     2401 cgatggtgaa ttgcccggtg ttgggatcaa gccgggg
//
LOCUS       DAOQLS010000293         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27001_length_2434_cov_4.971417, whole genome shotgun sequence.
ACCESSION   DAOQLS010000293 DAOQLS010000000
VERSION     DAOQLS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27001_length_2434_cov_4.971417"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..70
                     /locus_tag="PK362_06225"
     tRNA            1..70
                     /locus_tag="PK362_06225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31..33,aa:Pro,seq:ggg)
     gene            167..1300
                     /locus_tag="PK362_06230"
     CDS             167..1300
                     /locus_tag="PK362_06230"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC74463.1"
                     /translation="MPIPKRVLFCYISPASGHRRAAEAVMSVLRHGHPPIDCEGVNSI
                     SYANPVLGKLVSKLYLQVLKFAPQLWDFLYDNPHIEKATRDLRELLNMFNTRRIAEVL
                     RGFRPRCLVCTQAVPVGLLAALKERGKIKLPLVGVLTDFGVHNYWISPQVDLYLVPSE
                     EVRRKMIRVGVSESRIRVTGIPIDPHFGLPGDKRAERLALGLAPHRPTVLVMGGNYGL
                     GPMEDVVSSLRRLPVAPQVIAVCGSNRRLLGKMHRRFADDRSVRVMGQIRTIHRLMDA
                     ADLLISKSGGLTTAESLAKGLPMIIIQPIPGQEERNADHLLRYGAAERADTLEDLTRL
                     AQQLLSNRQRLDRLRENGRSLARPRAAYDAAEAIRALMEGGPR"
     gene            1297..2133
                     /locus_tag="PK362_06235"
     CDS             1297..2133
                     /locus_tag="PK362_06235"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC74464.1"
                     /translation="MNWEPLWALGLAGASRTVRRLPDRPRYACAAAIGRGVSRIFPDK
                     RAAVERNLAVINDWAGTRFRVEKVFENFGMTLSDFLAHPPVNIRVEGREKAEEARRHG
                     HGTIFLTSHLGNWELGGRVLAGWGWPVTAVYQPYTSKAMQKYIQDRRAPGLSYLAVGK
                     GAALGVARVLERRESVAILADRPFGEVGETVSLCGRPARLPRGPFLFAARSGAPLVPG
                     FVLMEGPGRYRVVVEDPIWPTGKGHAAVQILLDKMAQVLGKYLAQHAEQWYCFEPVWE
                     GS"
     gene            2150..2222
                     /locus_tag="PK362_06240"
     tRNA            2150..2222
                     /locus_tag="PK362_06240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2183..2185,aa:Pro,seq:cgg)
     gene            2252..>2434
                     /locus_tag="PK362_06245"
     CDS             2252..>2434
                     /locus_tag="PK362_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNC74465.1"
                     /translation="MERRRWRIFGRVQGVGFRAFCQREALERGVRGWVRNEPDGSVTA
                     EVEGPPATLDGLQAQLQ"
BASE COUNT          415 a          750 c          808 g          461 t
ORIGIN      
        1 gggcgtagcg tagtggtagc gcactcgctt ggggtgcgag aggtcgtggg ttcaatcccc
       61 accgccccga tatttttttt aaaagtgccg gaaggaccat gagtgaccgt tcagagcgtt
      121 ccttcccctt gaaagcgcca atccgaactt agaacgccgt ccacccttgc cgatcccgaa
      181 gcgcgtcctc ttctgctaca tctctcccgc cagcggtcac cgtcgcgcgg ccgaagccgt
      241 gatgtcggtc cttcgccacg gccatccgcc gatcgattgc gaaggggtca attccatcag
      301 ctacgccaat cccgtgctgg gaaagctggt ctctaaactc tatctccagg ttttgaaatt
      361 cgccccccag ctgtgggatt tcctttacga caacccccac attgaaaaag cgacccggga
      421 ccttcgggaa ctgctcaaca tgttcaacac ccgtcgcatc gccgaagtcc ttcgggggtt
      481 tcggccgcgg tgtttggtgt gcacccaggc ggtgccggtg ggcctcctgg cggcgttgaa
      541 ggaacggggg aaaatcaaat tgcccctggt gggcgttttg accgacttcg gcgttcacaa
      601 ttattggatc tccccgcagg tggaccttta cctcgtgccg agcgaggagg tgcgtcggaa
      661 aatgattcgg gtcggcgttt ccgaaagccg catccgggtc acgggcatcc ccatcgatcc
      721 ccatttcggt cttcccggcg acaaacgggc cgaacgcctc gctctgggat tggctcccca
      781 ccgccccacg gttttggtga tggggggaaa ttacgggttg ggccccatgg aggacgtggt
      841 gagctccctt cgacgattgc ccgtcgcgcc ccaggtgatc gccgtttgcg ggtccaatcg
      901 ccgcctgctc ggcaagatgc accggcgctt cgccgacgac cgttccgtcc gggtgatggg
      961 gcagatccgc accatccacc gtctcatgga cgcggcggac ttgttgattt caaaatcggg
     1021 cggtctgacc acggccgaat cattggccaa agggcttccc atgatcatca ttcaacccat
     1081 cccggggcaa gaggagcgaa acgccgatca tctgttgcgt tacggcgccg ccgaacgggc
     1141 cgacacgctg gaggatttga cgcgattggc ccagcagctt ctctcgaacc gacaacgcct
     1201 ggaccggctt cgggagaacg gccgttcctt ggcccgcccc cgggcggcct acgacgccgc
     1261 cgaggccatt cgggcgctca tggaaggcgg cccgcgatga attgggaacc cctgtgggcc
     1321 ctcgggttgg ccggcgccag ccgcacggtg cgccggttgc cggaccgccc ccgttacgcc
     1381 tgcgccgccg ccatcgggcg gggcgtcagc cggatattcc cggacaagcg ggcggcggtg
     1441 gaacgcaacc tcgcggtcat caacgactgg gcggggacgc gttttcgagt ggaaaaagtt
     1501 tttgagaatt tcggcatgac cctttcggat tttttggccc acccgcccgt gaacatccgg
     1561 gtcgagggcc gggagaaagc cgaggaagcc cgccgccacg ggcacggaac gatttttctg
     1621 acgtcgcact tgggcaattg ggaattgggc ggacgggtgt tggccgggtg ggggtggccc
     1681 gtgacggccg tttaccaacc ctacacgtcc aaggcgatgc agaaatacat tcaagaccga
     1741 cgggccccgg ggttgtctta tttggccgtc ggcaaaggag cggccctggg cgtggcccgg
     1801 gtgctggaac ggcgggaatc cgtggcgatt ctcgccgatc gccctttcgg cgaagtcgga
     1861 gaaacggtga gcctctgcgg ccgaccggcc cggctccccc ggggaccctt cctcttcgcg
     1921 gcgcggtcgg gcgcgccgtt ggtccccggc tttgtgctga tggaggggcc cgggcgctac
     1981 cgggtggtgg tggaggatcc catctggccc accggcaaag gccacgccgc ggtgcagatt
     2041 ctattggaca agatggccca agtattggga aaatatctgg cccaacacgc cgagcaatgg
     2101 tattgtttcg aaccggtttg ggaaggctct taacggcact gccggatccc ggggtgtagc
     2161 gcagctggta gcgcgcttgg ttcgggacca agaggtccag ggttcaagtc cctgcacccc
     2221 gagatccgga agcggccgat ggggggaaag ggtggaaaga cggcgttggc ggatcttcgg
     2281 ccgcgtgcag ggcgtggggt ttcgggcttt ttgccaacgc gaggccctgg aacgcggcgt
     2341 gcgcggttgg gttcgcaacg agcccgacgg cagcgtgacc gcggaagtgg agggtccgcc
     2401 ggcgaccctg gacgggttgc aggcccaact ccag
//
LOCUS       DAOQLS010000294         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27033_length_2433_cov_4.547939, whole genome shotgun sequence.
ACCESSION   DAOQLS010000294 DAOQLS010000000
VERSION     DAOQLS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27033_length_2433_cov_4.547939"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PK362_06250"
     CDS             <1..929
                     /locus_tag="PK362_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HNC74466.1"
                     /translation="PPRLLHYKKASTQSGAYKWWAVDGHYFLAAFLNEPAAPITLRVD
                     KDESFYSVHRPVPLVLEPKQSVTENLTFYLGPKGYEDLKKLGLDLERSVDFGFFAPVG
                     HVIHKSLLVFHRATRNYGWAIILLTLIIQLLVLPLTVTSFRHSQKMKTIQPQLKRLQE
                     LYKSDPQRLNVEMLALYQRHGLRFMGMEGCLPVLIQLPIFWALFSTLRSTYELRHAPW
                     IGWVKDLSVHDPYYVLPVLMGGGMFLQQKMSAAALDPNQRQIMYIMPVMFTFFFLKMP
                     AGLVVYWLTSSVISVAVQMVLLRLHAQPEAAR"
     gene            926..1996
                     /locus_tag="PK362_06255"
     CDS             926..1996
                     /locus_tag="PK362_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNC74467.1"
                     /translation="MKEMREWTGESRTAERAVERGLAVLGLKRDDVNVHVVQDRSSGL
                     LSLFGYRRVRVRIVEKRSARLDAYRDHGPDDDRFEHGRDDRGPRRGRHDRDDREETFE
                     KSGGKNGRRDRRNGPGGKNEDRPAPRGREDRRERDGRGANDRRPAEERRSTPKGPRGD
                     DRGNRSSERKNRPAPEPPRRSETRAPREQRAPRPEILRPFIPPETLLNQWKESLGIED
                     LAWEFGAEQNHRLPVLLKTTRGERLAGKNGRGLEALEYLFNLVSSGGDREKPWVALRL
                     EGFASADEQSVVDKALFAAFQVRRTQQPFRLDPMPPAQRRLVHQTLANHPDVETASEG
                     EGPTRKVVVKPRAAGAAPAPNA"
     gene            2025..>2433
                     /locus_tag="PK362_06260"
     CDS             2025..>2433
                     /locus_tag="PK362_06260"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNC74468.1"
                     /translation="MEPRETDTIVALATPPGEGGLGVVRLSGPRALAVANALFHAAAP
                     LLDVPSHTLHHGIVHDGTETMDDAVAAVFRAPRSYTGEDVVELSCHGGHLLLTRVLEL
                     CLARGARLAGPGEFTRRAYENGKMDLTQAEAVAE"
BASE COUNT          448 a          858 c          733 g          394 t
ORIGIN      
        1 aaccgccccg tctgctccat tacaagaagg cctcgacgca aagcggcgcc tacaaatggt
       61 gggccgtcga cggtcactat tttctggcgg cgttcctgaa cgagcccgcg gccccgatca
      121 cgctacgggt ggacaaggac gaatcgttct attccgttca tcgccccgtc ccgctcgtcc
      181 tggagcccaa gcaatccgtg acggaaaacc tcacgttcta tctgggcccc aaggggtacg
      241 aggacctgaa gaaactgggg ctcgacctgg aacggtccgt ggatttcggc tttttcgctc
      301 cggtgggtca tgtcattcat aaatcccttc tggtcttcca ccgggccacc cgcaactacg
      361 gctgggccat cattttattg acgttgatca tccaattgct ggtgctgccc ctgacggtca
      421 ccagcttccg tcacagccag aaaatgaaga ccatccagcc ccaactcaaa cgccttcagg
      481 agctctacaa aagcgatccc cagcgcctga acgtggaaat gctggccctc taccaacggc
      541 acggccttcg gtttatgggc atggaagggt gcctgccggt cctcattcaa ctccccattt
      601 tttgggcgtt gttttcgacc ctgcgcagca cctacgaatt gcggcacgcc ccctggatcg
      661 gttgggtgaa ggatttgtct gtccacgacc cctactacgt gctgccggtg ctcatgggcg
      721 ggggcatgtt ccttcaacaa aaaatgtccg ccgcggccct cgaccccaat caacggcaaa
      781 tcatgtacat catgcccgtc atgttcacct tctttttcct gaagatgccc gccgggcttg
      841 tggtttactg gctgaccagc agcgtgatca gcgtcgccgt ccagatggtg ctcctccgtc
      901 ttcacgccca acccgaggcc gcccgatgaa agaaatgcgg gaatggaccg gggaatcgcg
      961 aaccgccgaa cgcgcggtgg agcgcgggct cgccgtcctg gggctgaaac gggacgacgt
     1021 caacgttcac gtggtgcagg accgctccag cggcttgctg agtttgttcg gttaccggcg
     1081 agtgcgggtg cgcatcgtgg aaaaacgctc cgcccgattg gacgcctacc gcgaccacgg
     1141 gcccgacgac gatcggtttg agcacggccg cgacgatcgg gggccccggc ggggccggca
     1201 cgaccgcgac gaccgcgaag agacctttga aaaatccggc ggcaaaaacg gccgccggga
     1261 tcggcgcaac ggcccgggcg gcaagaacga ggaccgcccg gccccccggg gccgcgagga
     1321 ccgccgggag cgggacggcc gcggggccaa cgaccgacgg ccggcggagg aacgccgttc
     1381 cacccccaag gggccgcgcg gcgacgaccg cgggaaccga tcgtcggagc gaaaaaaccg
     1441 gccggcgccc gaaccgcccc gccggtcgga aacccgggcg ccccgggaac aacgggcccc
     1501 gcggccggag attttgcggc ccttcatccc ccccgaaacc ctcttaaacc agtggaagga
     1561 atccctcggc atcgaggatc tcgcctggga gttcggcgcg gagcaaaacc accgtctccc
     1621 ggtcctcctc aaaaccaccc ggggcgaacg gttggccggg aaaaacggcc ggggcctgga
     1681 agcgctcgaa tacctcttca acctcgtgtc ttccggcggc gaccgggaaa aaccctgggt
     1741 cgccctccgc ctggaagggt tcgcctccgc ggacgagcaa agcgtggtgg acaaagccct
     1801 tttcgccgcc tttcaggttc gacgcaccca acaacctttt cgtttggatc ccatgccgcc
     1861 ggcccagcga cggctcgtgc atcaaaccct ggccaaccat cccgacgtgg agacggcgtc
     1921 cgaaggggaa ggcccgaccc gaaaagtggt cgtgaagccc cgcgccgccg gggccgcccc
     1981 cgcgcccaac gcctaaggat tttttccctc gggcgggccc tcccatggaa ccccgcgaaa
     2041 ccgacaccat cgtggccctc gccaccccgc ccggcgaagg cggcctgggc gttgtgcgcc
     2101 tgtccggtcc ccgggccttg gccgtggcca acgccctgtt ccacgccgcg gcgcccctcc
     2161 tcgacgtccc ctcccacacc ttgcaccacg gcatcgtcca cgacgggacg gaaacgatgg
     2221 acgacgccgt cgccgccgtc ttccgcgccc cgcgctccta caccggcgag gacgtcgtgg
     2281 aattgtcctg ccacggggga cacctcctgc tcacccgcgt gctggaactc tgcctcgcgc
     2341 gcggagcccg gttggccggt cccggcgaat tcacccgtcg ggcctacgaa aacggaaaaa
     2401 tggatctgac ccaggccgag gccgtggccg aat
//
LOCUS       DAOQLS010000295         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27061_length_2432_cov_2.683214, whole genome shotgun sequence.
ACCESSION   DAOQLS010000295 DAOQLS010000000
VERSION     DAOQLS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27061_length_2432_cov_2.683214"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..>2432
                     /locus_tag="PK362_06265"
     CDS             233..>2432
                     /locus_tag="PK362_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74469.1"
                     /translation="MKTFIPNTVRIVADVTAFNFYQASVGGLVLTAAGGEKVSLFGGV
                     VEINAFSSLSDWKGMISSTSDRMTSSVKDPGMWGFGAGVMFFNPVLSSVLQKYIPVLS
                     PVMKALSANENIAGGLLSRGMAGRIQGFIVEEGIKEALPEKVMKGVGLGDTLASEVFQ
                     EMFDKRGGMRMANTKSNFASVQSKYDGVFSVMANNASSYSPNAENVVNAVSTALQEAP
                     TTIRSSINDLLTAKPDAIARADQSSLGVPGGLTETGHAVTGTAPASETAGSPVNLRAE
                     GEGSFSTVGRSLGTEQGNNVEFAGLAVMLSASDTEMQQALYNLAAGPMEGAGSFREVS
                     DYLESARDVINTAGVARTDLLSAAREGAGQFLAAVANNTPAFVNSAQGVAVLTRFVQE
                     SLATVGYEGNGKTFDKLISALSNVSASVASTESLTALTNLTAQLLYKDGPENMPLGTI
                     NNLTYGLLSGISNLMDSPTLETELAEKNEGTLNAATALKVLTLQIDQQPMISFKARWA
                     EVIGQHQSLLARTSEPATNIFDLFLLQTAANLDQSDLQKAVRELPKNARAQDLANSLR
                     LLHGVRTQVLGLGVDELPGAPADPILKSIYERDIALVAHLRTLGGEALLKQVLAQPLK
                     KLEVLAGNDSTEKIRFLFNAGLPTVRSMETIADRNTVLALKTTKLLGFGANKFALASG
                     AGLAVTTVASALAMPLGMATAPLLAAAAAASYSLVAMGVGMAADWTHGQFS"
BASE COUNT          525 a          718 c          689 g          500 t
ORIGIN      
        1 gggtatggcc gatgcgctta atacggccac cgctaaaggc atgatcgctt ccgttagaga
       61 atgggcttcc actgcaacgt tttccagcac cgcgcggtcc attggctccg gtgtttcaac
      121 gttgctgggc gcccagaact acaccgccct gggtaaagaa atatcggaag ttggccttaa
      181 aacggtattc ggtaaatggc tttcgaccgc atcggaaggc atcaaagaag ggatgaaaac
      241 ctttattccg aacacggttc gcatcgttgc cgacgtcacc gccttcaact tctatcaagc
      301 gtcggtcggt ggtcttgttt tgacggcggc cggaggggaa aaggtttccc ttttcggcgg
      361 ggtggtggaa atcaacgctt tctccagtct ctccgattgg aaagggatga tttcaagcac
      421 cagcgatcgc atgaccagct cggtcaaaga ccccggcatg tggggctttg gcgcgggagt
      481 catgttcttt aacccggttc tttcttcggt cttgcaaaaa tacattccgg tgctcagccc
      541 cgtcatgaaa gccctttcgg ccaacgagaa catcgccggc ggattgctga gccgcgggat
      601 ggccggtcgc atccagggct ttatcgtcga agaaggcatt aaagaagccc ttcccgaaaa
      661 ggttatgaaa ggcgtcggcc ttggggatac gttggccagc gaagtctttc aagaaatgtt
      721 tgataaacgc ggtggtatgc ggatggcgaa caccaagtcg aacttcgcca gtgttcagtc
      781 caaatacgat ggggttttca gcgtcatggc gaacaacgcc tcctcctatt cgcccaacgc
      841 tgaaaacgtt gtcaacgcgg tttcgaccgc cctacaggaa gcccccacaa cgattcgcag
      901 ttccatcaac gatctgctca cggcgaagcc cgacgcaatc gcgcgggccg accaatcgtc
      961 gctgggagtc cccggcggct tgaccgaaac gggccatgcc gtgacgggaa cggcgcccgc
     1021 ttccgaaaca gcaggcagcc ccgtgaacct gcgggccgag ggagaaggat ctttctccac
     1081 cgttgggcgg tcgcttggca cggaacaagg caacaacgtg gaattcgccg gcctcgccgt
     1141 gatgctttcc gcgtccgaca ccgaaatgca acaagcgctg tataacctgg cggccggccc
     1201 catggagggg gcgggttctt tccgcgaagt ctccgattac ctggaatcgg cccgagacgt
     1261 gatcaacacc gccggtgtcg cccggaccga ccttctctcc gcggcccggg aaggcgccgg
     1321 tcaattcttg gccgccgtgg ccaacaacac ccccgccttt gtgaattcgg cgcaaggcgt
     1381 cgcggtgttg acccgttttg tccaggaatc tctggcgacg gtcggatacg aagggaacgg
     1441 aaaaaccttt gacaaattaa tttccgcgct gtccaatgtt tcggcgagtg tcgcttccac
     1501 cgaatcgttg accgcgttga ccaatctcac cgcgcagttg ctctacaaag acggcccgga
     1561 gaatatgccc ctggggacca tcaacaacct cacttacgga cttctgtccg ggatttcgaa
     1621 cctgatggat tcacccaccc tcgaaaccga attggctgaa aagaacgaag ggacattaaa
     1681 cgccgcaacg gccttaaagg tgctgacgct ccaaatagat cagcagccga tgatatcctt
     1741 caaggctcgc tgggcggagg tgattggtca acaccaatcg cttctggccc gaaccagcga
     1801 acccgccaca aatattttcg acttgttctt actgcagacg gccgccaacc ttgaccaatc
     1861 cgatctgcaa aaagccgttc gggaacttcc caaaaacgcc cgggcacaag accttgccaa
     1921 cagcctccgc ctcttgcacg gcgttcggac ccaagtcttg ggtctcggcg ttgacgaatt
     1981 gcccggagcc ccggccgacc ccatcctgaa gtccatttac gagcgggaca tcgcgctcgt
     2041 ggcacacctt cggacgttgg gaggggaagc gcttttgaag caagtcctcg cgcaacctct
     2101 gaaaaagctg gaggttttgg ccggaaatga ttccacggag aaaatccggt tcttgttcaa
     2161 cgccggactt ccgaccgttc gatccatgga gacgatcgcg gatcggaaca ccgttctcgc
     2221 gttgaaaaca accaaactgt tggggtttgg cgccaacaag tttgctttgg cctccggcgc
     2281 tgggttggcc gtgaccacgg tggcaagcgc gctggcgatg cctctcggca tggcgacggc
     2341 accgctgttg gccgccgcgg ccgcggcgag ttactctctc gttgccatgg gagtgggcat
     2401 ggcagccgat tggacccatg gccaattcag tc
//
LOCUS       DAOQLS010000296         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27132_length_2428_cov_4.347661, whole genome shotgun sequence.
ACCESSION   DAOQLS010000296 DAOQLS010000000
VERSION     DAOQLS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27132_length_2428_cov_4.347661"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PK362_06270"
     CDS             complement(<1..690)
                     /locus_tag="PK362_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC74470.1"
                     /translation="MSFAASDRLGQLPPYLFAAIDAKKKAAQEKGADIISLGVGDPDL
                     PTPAHIIEAGKRGLENPANHQYPFGAGLMSFRRAVADWYRKRFNVGLNPETEIHSLIG
                     SKDGLTHLPLAFLNPGDLALIPEPAYPAYNAAVLLAGGRPHFVPLLEKNKFLPDFDAV
                     PKDVLGKAKLLFINYPSNPISVTAPRSFYESVVALARKHKFLVVHDAAYSEMYYETPP
                     MSFLEVPGAKEV"
     gene            complement(687..1325)
                     /locus_tag="PK362_06275"
     CDS             complement(687..1325)
                     /locus_tag="PK362_06275"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNC74471.1"
                     /translation="MAEPSTSPVGRGRLEAFSDGVFSIVITLLVLDLRLPEGDVDSAG
                     YLVWKILALWPQYFGYVLSFLIVGIFWVAHHFIFHHIRRVDRPLLWMNIFFLMALAFL
                     PFPTTLISRHWAWAPSVWAYGGTLCAANLLLNAMWRYATRGGRLVDPSLSPELVRDVN
                     RRSLRAVAVYVAAMLVAAWSPRVGLAVFLIVHILYILPAALDRHWTPKENHP"
     gene            complement(1318..1719)
                     /locus_tag="PK362_06280"
     CDS             complement(1318..1719)
                     /locus_tag="PK362_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74472.1"
                     /translation="MNLKKAWSALTHLLDEEDPATELIYDPVDVGAVIVGTLAGVGIL
                     YWLLWSLLVCEGGLFGKVIPFLQVVFTDKTLQDFGYEGYPYELGLFEGWVVNVVALVA
                     AAALLYGVWWVLYAPKHRPDASRKNRDRSHG"
     gene            complement(1720..>2428)
                     /locus_tag="PK362_06285"
     CDS             complement(1720..>2428)
                     /locus_tag="PK362_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNC74473.1"
                     /translation="RLDGSWIEEILPDPFGPHEASGLRWKLSAVRLLPPCAPSKIVAV
                     GVNYADHAKEFGKAPPPEPLIFLKPPSAVIAPGEKIRRPRRSTQVDFEGEIAVVIGRR
                     ARSVSKARALEYVLGYTLMNDVTARDLQRLDGQWSRAKGFDTFAPLGPWIATGLRPRR
                     LKIETFVNGRRRQSSSTAQLIFDVPTLVSHISGVMTLEPGDVISTGTPSGVGPLRRGD
                     RVEIRCPQIGRLINKVA"
BASE COUNT          512 a          720 c          794 g          402 t
ORIGIN      
        1 cacctccttg gcccccggga cttccaaaaa ggacatgggc ggcgtttcgt aatacatttc
       61 cgagtaggcg gcgtcgtgca ccacgagaaa tttatgtttc cgcgccaggg ccaccacgga
      121 ttcataaaac gagcgcggcg cggtgaccga gatggggttg ctcgggtaat tgatgaagag
      181 cagcttggct tttcccaaaa catccttggg gacggcgtcg aaatccggca aaaacttgtt
      241 cttttccaac agaggcacaa agtgggggcg tccccccgcc aaaagcacgg ccgcgttgta
      301 ggccgggtag gcgggttcgg gaatgagcgc caagtccccc gggttcagga acgcgagcgg
      361 cagatgggtg agcccgtcct tggagccgat cagggaatgg atttctgtct cgggattcaa
      421 tccgacgttg aatcgtttcc ggtaccagtc cgccaccgcc cggcggaaac tcatcaggcc
      481 cgcgccgaag ggatactggt ggttggccgg gttttccaga ccgcgtttgc ccgcttcgat
      541 gatgtgggcc ggggtgggca ggtccgggtc gcccacgccc agggagatga tgtcggcgcc
      601 tttttcctgg gcggcctttt tcttggcgtc gatggcggcg aacaaatagg ggggcagttg
      661 gcccaaacga tcggaagcgg cgaagctcat gggtggttct ccttcggggt ccaatgccgg
      721 tcgagggcgg ccggcaaaat gtagagaatg tgtacgatca agaaaacggc gaggccgacc
      781 cgggggctcc aggccgcgac gagcatggcg gcgacgtaaa cggccacggc tcgaagggaa
      841 cgccggttga cgtcgcgaac caattcgggg gagaggctcg ggtccaccaa ccgcccgccc
      901 cgggtggcgt agcgccacat ggcgttcaac aacaaattcg cggcgcacag ggtgcccccg
      961 taggcccaca ccgaaggcgc ccaggcccaa tggcggctga tgagcgtcgt cggaaaggga
     1021 agaaaagcca gggccatgag gaagaaaata ttcatccaga ggagcggccg gtccacccgg
     1081 cgaatgtggt gaaagatgaa atggtgggcc acccagaaaa tcccgacgat cagaaaactg
     1141 agaacgtacc cgaaatactg gggccacagg gcgaggattt tccacaccag ataccccgcc
     1201 gagtccacgt cgccttccgg caggcgcaga tccaacacga gcagggtgat gacgatggaa
     1261 aaaacgccgt cggagaaggc ctccagtcgg ccccgaccca cgggagaagt cgacggttca
     1321 gccatgggag cgatcccggt ttttccggga ggcgtcgggc cgatgtttcg gcgcgtacag
     1381 gacccaccaa accccgtaaa gcagggccgc cgccgcgacg agcgcgacga cattgaccac
     1441 ccacccctcg aacaacccga gttcgtaggg atacccttcg tagccgaaat cctggagcgt
     1501 tttgtccgtg aagaccacct gaaggaaagg gatcactttt ccgaacagcc ccccttcgca
     1561 cacgagcagg gaccacagaa gccagtagag gattccaacg ccggccaggg tgccgacgat
     1621 caccgcgccc acgtccaccg gatcgtagat caattccgtg gcggggtctt cctcgtccag
     1681 aaggtgcgtc agggcgctcc acgctttttt taagttcatc taggccacct tattgattaa
     1741 tcgaccgatt tgagggcaac gaatttccac ccgatccccg cgacgaaggg ggccgacccc
     1801 ggagggcgtt ccggtggaga tgacgtcgcc gggttccagc gtcatgacgc cgctgatgtg
     1861 ggacacgagc gtcggcacgt cgaaaatcag ttgggccgtg gaggacgact gccgccgacg
     1921 gccgttcacg aaggtttcga tcttcagccg ccggggccga aggccggtgg cgatccaggg
     1981 gccgaggggc gcaaaggtgt cgaaaccctt ggcccgggac cattgaccgt ccaggcgttg
     2041 caagtcccgg gcggtcacgt cgttcatcaa ggtgtatccc agcacgtatt ccagggcccg
     2101 ggctttggaa accgaacgcg cccggcgacc gatcacgacc gcgatttccc cctcgaaatc
     2161 aacttgtgtg gaacgccggg gacgacggat cttttcgcca ggggcaatca ccgcgcttgg
     2221 gggttttaaa aaaatcagcg gctccggggg cggcgctttc ccgaattcct tggcgtggtc
     2281 ggcgtaattg acgccgacgg ccacgatctt ggagggcgcg cagggcggca aaagccgcac
     2341 cgccgaaagt ttccaccgaa gcccggaggc ctcgtggggg ccgaaggggt cgggcaggat
     2401 ctcctcgatc cacgaaccgt cgaggcgg
//
LOCUS       DAOQLS010000297         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27185_length_2426_cov_3.717841, whole genome shotgun sequence.
ACCESSION   DAOQLS010000297 DAOQLS010000000
VERSION     DAOQLS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27185_length_2426_cov_3.717841"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PK362_06290"
     CDS             complement(<1..331)
                     /locus_tag="PK362_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74474.1"
                     /translation="MNCRETRMELTAATAGGSLPPDARVHLEGCAECRAEGAAVSSFL
                     AQWRRGESPADDLFIQQRARVMERLGPAARPSRLWRWVPVAAVLLVLGIWKSQHQRRP
                     APPSAECV"
     gene            complement(328..924)
                     /locus_tag="PK362_06295"
     CDS             complement(328..924)
                     /locus_tag="PK362_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC74475.1"
                     /translation="MAGPLGMEEQWIVDSKAGDPAAFGQLVAAHQKQLFGFLYSYTGR
                     RDVADELTQEAFLKAWGGMKGFRAESRFQTWLFQIGLNLLRSWGRRETVRRWREVALV
                     FSRPEDERPRSVADLAPDERADADPHRNAEGRERGAALRVALARLPPKEKSVFLLRHE
                     QGMALADIAAALGIAEGTVKAHLFHAVQKLRRTMEEGT"
     gene            1193..1621
                     /locus_tag="PK362_06300"
     CDS             1193..1621
                     /locus_tag="PK362_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74476.1"
                     /translation="MPESLAMHPLDEGLFPEREKEPGVYVIEASVVRPEADPRLALPA
                     GCSAALLVSSTLLAEGVPPGASWNLEVRSKTRRRTFRLESPVRRGGNVLPIDPAPGPR
                     PKGTVDRALFWVDLTPLALAPGDLIVLVHGPAKTELPFPR"
     gene            complement(1637..1924)
                     /locus_tag="PK362_06305"
     CDS             complement(1637..1924)
                     /locus_tag="PK362_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74477.1"
                     /translation="MNKQWAVGALAILLGSGWTVAAAEKKGKSAKADKTEVVESTPTA
                     KKSHPVVDAVMDEVKDEAKEEALDRLEGKGSGDSAGEKIKKKAIRFGRQIP"
     gene            complement(1988..2341)
                     /locus_tag="PK362_06310"
     CDS             complement(1988..2341)
                     /locus_tag="PK362_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNC74478.1"
                     /translation="MGSKDRSFLDYVLRDALARVPGVVGRPMFGGWGIYQGPRFFGIV
                     FKGRLFFKVSEDTVAGYRERGSEPFSPSARQTLKSFYEVPSEVLDAPPLMADWARDAI
                     RAAGLPKKKAGGRSK"
BASE COUNT          403 a          859 c          696 g          468 t
ORIGIN      
        1 ggacgcattc cgcgctgggc ggcgccggcc gccgttggtg ctgggatttc caaattccga
       61 ggaccagcag gaccgccgcc accggcaccc accgccacag gcgggacgga cgggcggccg
      121 ggcccaggcg ttccatcacc cgcgcccgct gttgaatgaa caaatcgtcg gcgggggatt
      181 cgccccggcg ccattgcgcc aggaaagacg acaccgccgc cccttcggcg cggcattccg
      241 cgcagccctc caagtggacc cgggcgtcgg gcggcaggga accgccggcc gtcgcggcgg
      301 tcaattccat tcgggtctcc cggcaattca tgtcccttcc tccattgtcc gacgtaactt
      361 ttgaaccgcg tggaacaaat gggctttcac ggttccctcc gcgattccca gggcggcggc
      421 aatgtccgcc aaggccattc cctgttcgtg ccgaagcaaa aacacggatt tttccttggg
      481 cggcagtcgg gccagggcga cccgaagggc cgctccccgt tctcgcccct cggcgttccg
      541 atgggggtcg gcgtccgccc gttcgtccgg cgccaaatcc gccaccgatc ggggcctttc
      601 gtcttccggc cgggagaaaa ccagcgccac ctcccgccac cggcggacgg tttcccgccg
      661 cccccaactt cgcaacaggt ttagtccaat ctggaaaagc cacgtttgaa accggctctc
      721 cgcccgaaac cctttcattc cgccccaggc cttgaggaac gcctcctggg tcagctcgtc
      781 ggcgacgtcc cgccgccccg tgtaggaata aaggaaccca aaaagctgtt tttgatgtgc
      841 cgccaccaat tgaccgaagg ccgcggggtc gccggctttg gaatcaacga tccattgttc
      901 ttccatgccc aggggtccag ccattggacg ctttccgatc caaaaaggtt tagtctcagt
      961 aagggttgat tcgctgcgaa gggattttac cactatccct gggagaaggc ccggtccccc
     1021 gggggtcctt gcggatggct tccaggaaaa ttctacaatc aaatcgtcca tgcgccccga
     1081 cgattcgccg ttcggtcacc gttcattggc ccaacagggc cgcccctccc gggcgtgggc
     1141 gctcgccctg ggagcggtcc tgcttttatc cgtccccgcc cgggaggccc cgatgccgga
     1201 atcgcttgcg atgcacccct tggacgaagg tctttttccg gagcgggaga aggaacccgg
     1261 ggtttacgtt attgaagcgt ccgtcgtgcg cccggaagcg gacccccgac tggcccttcc
     1321 cgccggctgc tccgccgccc tgctggtcag ctccacgctt ttggccgaag gcgttccgcc
     1381 cggggcctcc tggaacctgg aggtgcgaag caaaacccgc cgccgaactt tccgtcttga
     1441 aagtccggtg cgccggggcg gaaacgttct tcctatcgac cccgcccccg gcccccggcc
     1501 gaagggcacc gtggaccgcg ctcttttctg ggtggacctg acccccctgg ccctggcccc
     1561 gggcgacctc atcgtcctgg tccacggacc cgcgaaaacc gaactgccct tcccccgtta
     1621 agtcgggacg ccggagttaa ggaatctgcc gaccgaaacg gatcgctttc tttttgattt
     1681 tctcccccgc gctgtcgccg gaacctttgc cttccaatcg gtcgagggct tcttccttgg
     1741 cctcgtcttt cacttcgtcc atcaccgcgt ccaccacggg gtggctcttt ttggccgtcg
     1801 gggtcgattc gaccacttcc gttttatccg ccttggcgga tttgcctttc ttttccgccg
     1861 ccgcgaccgt ccacccgctc ccgagcaaaa tcgccagcgc acccaccgcc cattgcttgt
     1921 tcattgattc ctccgttcat cgttttggat ttcccattat ggatgggaac ccctcgaaaa
     1981 acaatcctta tttcgaccgg cccccggctt tctttttggg aaggcccgcc gcccggatcg
     2041 cgtcccgggc ccaatcggcc atcaacgggg gcgcgtcgag cacctcagag ggaacttcgt
     2101 agaaggattt cagggtttga cgcgccgacg gcgagaaggg ttcggaaccc cgctcccggt
     2161 aaccggcgac cgtgtcctcc gacaccttga aaaacagccg tcctttaaag acgatcccga
     2221 aaaaccgggg gccctggtaa atcccccacc cgccgaacat gggcctcccg acgacgcccg
     2281 gcacccgggc cagggcgtcc cgaagaacgt agtcgagaaa ggaacggtcc ttggacccca
     2341 tggccctatt tcgcgggcgg acccgccgcc ttggggggct taagatcgat ggcccagaga
     2401 atccgaccgg tggtgaattc cacccc
//
LOCUS       DAOQLS010000298         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27214_length_2424_cov_4.320388, whole genome shotgun sequence.
ACCESSION   DAOQLS010000298 DAOQLS010000000
VERSION     DAOQLS010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27214_length_2424_cov_4.320388"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PK362_06315"
     CDS             <1..711
                     /locus_tag="PK362_06315"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74479.1"
                     /translation="RAVGLSTDEAQKVRDFLLAYSVQSEFFDPSGRSASLGQRIVRIA
                     QLGVDEKEEPVFEFRSPILARGRYDIQYERLQTLLRTTRLSPQERRHLKAFVRRLELI
                     NWRQNTLYRILDFVCHTQRHFLAHRDGLKKIPITQRQLAKKLAVAPSTINRAIQGRSL
                     VLPWGEEVLLEELFCSRKSLCVEALGSLEAEDVQFDRRTDQELQERLQARLGFPVPRR
                     TLNSYRRALESGHDAHAA"
     gene            783..1199
                     /locus_tag="PK362_06320"
     CDS             783..1199
                     /locus_tag="PK362_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC74480.1"
                     /translation="MAPPGFTLIELMIVVAILGILAAVAIPKFAGLINKSREAQAKYG
                     LGSVRSSLSLYYADNQFFPTVGGFSSAVLGRYIDKIPPVLIPPPGDHPRSETVGDYLG
                     TINDTGIWLYDITGTTGRILIDCSHQDQQGRTWSTW"
     gene            1365..1841
                     /gene="rpsF"
                     /locus_tag="PK362_06325"
     CDS             1365..1841
                     /gene="rpsF"
                     /locus_tag="PK362_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNC74481.1"
                     /translation="MLLYETVFVVPADLSPQKTDEYVEKIKNMITAKGGEVVLVDKWG
                     RRRLSYPIRRHREGFYTFLQFKAPSAFIAELNQFFRVSEEVIRQVICKALKGKPASPT
                     MSLPPVAPGSHGFGGHGSHGPRSHGAPAMTAAPAAAPAAAPTEESHGQPAPAAPAQ"
     gene            1807..2253
                     /gene="ssb"
                     /locus_tag="PK362_06330"
     CDS             1807..2253
                     /gene="ssb"
                     /locus_tag="PK362_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC74482.1"
                     /translation="MANPLRLLQLNNVTLVGRLTRDPELRFTTSGAPVCRFDLAVNRR
                     YKDKAGEWKDDTSFVPVVVWREAGQRCGEKLKKGSPVFVEGRLKSKSWETKDGQKRSG
                     LEVDALRVQFLEKAEGAPTTDEEPSAPDAEIGAEAPAAAGAEEVPF"
BASE COUNT          454 a          835 c          692 g          443 t
ORIGIN      
        1 cgggccgtgg gcctctccac ggacgaggct caaaaggtgc gggatttcct cctggcctat
       61 tcggtccaat cggaattctt cgatccttcc ggacggtccg cctccctcgg ccaacgcatc
      121 gtgcggatcg cccaattggg cgtcgatgaa aaggaggagc cggtcttcga attccgctcc
      181 ccgatcctgg cccggggccg ctacgacatt caatacgagc ggttgcagac cctcctgcgc
      241 acgacccgac tctcgcccca ggagcggcgc catttgaagg ccttcgtccg tcgtctggag
      301 ctgatcaact ggcgtcaaaa caccctctac cgtattttgg attttgtctg ccacacccaa
      361 cgtcattttc tcgcccaccg ggacggttta aagaaaatcc ccatcacgca acgccagttg
      421 gccaaaaagt tggccgtggc gccctccacc atcaaccggg ccattcaagg gcgcagcctc
      481 gttcttccct ggggggagga agtcctgctg gaggaattgt tttgttcccg gaaatcgctg
      541 tgcgtggagg ccctgggaag tttggaggcc gaggacgtgc aattcgatcg ccgcaccgac
      601 caggagcttc aggaacgcct ccaggcccgg ctcgggttcc cggttccccg ccgcaccttg
      661 aacagttatc gtcgggccct ggaatccggc cacgacgccc acgccgctta aaacaacggg
      721 gcgatttttc cattgatttt tgattcgata gcggttatgc tttcccgatc atgagacggc
      781 gtttggctcc ccccggattc accctcatcg agttaatgat cgtcgtcgcc attctgggca
      841 ttctggccgc cgtcgccatt ccgaaatttg cggggttgat caacaaatcc cgggaagccc
      901 aggccaaata cggcttggga tccgttcgct cctccctcag cctttattac gcggacaatc
      961 agtttttccc caccgttggc gggttctcca gcgccgtcct gggacgctac atcgataaaa
     1021 tccctccggt tctcatcccc ccgccgggcg accacccgcg gtcggagacc gtgggagatt
     1081 atttgggaac gatcaacgac accggtattt ggttgtacga catcaccggc accaccggcc
     1141 ggatcttgat cgattgttcc caccaggacc agcagggacg cacctggagc acctggtagt
     1201 cttcccttcc cgggggcgaa tcccgcctcc cttgccaatc tcccgccgaa tagtgtagaa
     1261 tagcgccact ttggttccgg tcggtcgctg agcccggagc ccaacttcac ggggaacttg
     1321 accgccgcgc ggtcgtccgc gaagagccca caaaggaggc tgaattgcta ctgtacgaaa
     1381 ccgtgttcgt tgtgcccgcc gatctctcgc cgcaaaaaac cgacgagtat gttgaaaaaa
     1441 tcaaaaacat gatcaccgcc aaaggcgggg aagtggtcct ggtggacaaa tggggccgcc
     1501 gccgtctgtc ctacccgatc cgccgccacc gggaagggtt ctacaccttt cttcaattca
     1561 aggcgccctc ggcgttcatc gccgagctca accaattctt ccgcgtgtcg gaagaagtca
     1621 tccgtcaagt gatttgcaaa gcgctcaagg gcaaacccgc gtcgccgacc atgtcgttgc
     1681 cgccggtggc cccgggctcc cacgggttcg gcggccacgg ttcccacggc ccccggtccc
     1741 acggcgcccc ggcgatgacc gccgcgcccg ccgcggcgcc cgcggccgcg cccacggagg
     1801 aatcccatgg ccaacccgct ccggctgctc cagctcaata acgtcaccct ggtcggacgt
     1861 ctgacccggg accccgaact ccgcttcacg acttcgggcg cccccgtctg ccgtttcgac
     1921 ctggccgtca accgccggta caaagacaag gcgggggaat ggaaggacga cacgtccttc
     1981 gttcccgtcg tggtctggcg cgaagcgggc caacggtgcg gggagaagct caaaaaaggc
     2041 agccccgtgt tcgtggaagg acgcctcaag agcaagtcct gggaaaccaa ggacggccaa
     2101 aagcggtccg gcctcgaagt ggacgccttg cgcgtgcagt ttttggagaa agccgagggg
     2161 gccccgacca ccgacgaaga accgtcggcc cccgacgcgg aaatcggcgc cgaagcaccg
     2221 gccgccgcgg gcgctgaaga agtgcccttt taagaaccct ttaggagaac gaaaatgacc
     2281 gaatccttgc cccccacgac ccccgcggcc cccacggccg gcgcgggcga caaagccccg
     2341 gtttccaccg gtcccaagcc cttcggcgcg cgccccggcg cccggccgcc cttcggcggc
     2401 ccccgttccg gccccggcgg tccc
//
LOCUS       DAOQLS010000299         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27279_length_2420_cov_5.134461, whole genome shotgun sequence.
ACCESSION   DAOQLS010000299 DAOQLS010000000
VERSION     DAOQLS010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27279_length_2420_cov_5.134461"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /gene="ftsZ"
                     /locus_tag="PK362_06335"
     CDS             complement(<1..1020)
                     /gene="ftsZ"
                     /locus_tag="PK362_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNC74483.1"
                     /translation="MVQIKFTQEFNEQPANIKVMGLGGAGGNAINRMIEAGLSHVEFI
                     AANTDSQALRRNLAPVRLQLGETATKGLGVGGNPALGRQATEESRDRVQEILTGADMV
                     FITAGMGGGTGTGSAPLVAQIAKSLDPKPLVVGVITRPFEFEGLVRKNQADQGIEELR
                     PHVDTLLAIPNDRLFEIIDENTTSIEAFRVADNVLRQAVQAITDVITRHGLINVDFAD
                     VRSIMSGAGEALMGMGESRGPGRAVEAAKKAIHSPLLENLSIDGAKGLLVNIAGNKSI
                     TLHEVKSAMDYIKNAASPEAHVFYGQVFDDGLEDRFCVTVIATGFPPARKATALRRVG
                     RDAADP"
     gene            complement(1026..2267)
                     /gene="ftsA"
                     /locus_tag="PK362_06340"
     CDS             complement(1026..2267)
                     /gene="ftsA"
                     /locus_tag="PK362_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNC74484.1"
                     /translation="MPKSDLITGLDVGSGQVVAVVAEQAPDADVPEIRGAARQPCAGL
                     KGGVVINIDETARAVTRAVEAAEEMAGLAGASRRVLIGVRGAHIQTFNHHGAMNIART
                     DKEITVSDRDQVVENTKAVPISPDREIVHVIPQDFILDRQSGVPNPVGMEASLLEVDV
                     HIVTASQSHLNNVWKAIARAGFEVEEPIYGLLAVGDTVVSAEEKGLGCLLVDLGGQTT
                     GLAVYAEGSVRFTKELALGSDNISHDLSHALRTTLSQAHKVKERFGAASRQWAEGNVD
                     EEIEYTSVDGRTPRRVKRQALFDYIAPRVEEIFTLIGEELQRSNYADHVAGGGVVLTG
                     GGAQLQGLAAAAEQILDVPVRIGLPQGLQGPPDVLGHPGYATALGLVTYRHGGDWSRS
                     RRAVRSVGFGQRLKSFVEDFF"
BASE COUNT          366 a          766 c          825 g          463 t
ORIGIN      
        1 cgggtccgcg gcgtcgcggc cgacccggcg gagcgccgtg gccttccggg cgggcgggaa
       61 gccggtggcg atgacggtca cgcaaaaacg gtcttccaaa ccatcgtcga aaacctggcc
      121 gtagaaaacg tgggcttcgg gggacgccgc gtttttaatg tagtccatgg cgcttttgac
      181 ttcgtgcaag gtgatggatt tgttgccggc gatgttgacc aggagccctt tggcgccgtc
      241 gatggacaaa ttttccaaca ggggcgaatg gatggctttt ttcgcggcct ccaccgcccg
      301 gcccggaccc cgggattcgc ccatgcccat cagggcctcg cccgctcccg acatgatgct
      361 gcgcacgtcc gcgaaatcca cgttgatcag gccgtgccgg gtgatcacgt cggtgatggc
      421 ctgcacggcc tggcgcagga cgttgtcggc cacgcgaaag gcttcgatgg aggtggtgtt
      481 ttcgtcgatg atttcgaaca ggcggtcgtt gggaatggcg agcagggtgt cgacgtgggg
      541 gcgcaattcc tcgatgccct ggtcggcctg gtttttccgg accagaccct cgaattcgaa
      601 agggcgggtg atgaccccga ccacgagcgg cttggggtcg aggcttttgg cgatttgggc
      661 caccaggggg gccgatcccg tgcccgtgcc gccgcccatg ccggccgtga tgaagaccat
      721 gtcggcgccg gtcaagattt cctggacgcg gtcccggctt tcctcggtgg cttggcgtcc
      781 cagggccgga ttgccgccca cgcccagccc tttggtggcg gtttcgccca attgaaggcg
      841 gacgggggcc aggttgcgcc gaagcgcctg ggagtcggtg ttcgccgcga tgaactcgac
      901 gtgggagagc cccgcctcga tcatccggtt gatggcgttg ccgcccgcgc cgcccaagcc
      961 catcactttg atgttggccg gctgctcgtt gaactcctgg gtgaatttga tctggaccat
     1021 ggcgtttaga aaaagtcttc cacgaaggat ttgaggcgtt gaccgaaacc caccgaccgg
     1081 acggcccggc ggctgcggga ccaatccccg ccgtggcggt aggtgacgag gcccagggcc
     1141 gtggcgtacc cggggtggcc gagaacgtcc ggcggtcctt gaagaccctg gggaagtccg
     1201 atgcggaccg gcacgtccaa aatttgttcg gcggccgcgg ccagcccctg caattgggcg
     1261 cccccgccgg tgagcacgac cccgccgccg gccacgtgat cggcgtaatt ggaccgttga
     1321 agttcttcgc cgatcaacgt gaaaatttcc tccacccggg gagcgatgta atcgaaaagc
     1381 gcctggcgtt tgacccgacg gggggtccga ccgtcgacgc tggtgtattc gatttcctcg
     1441 tccacgttgc cttcggccca ttgacgggac gccgccccaa accgttcctt gactttgtgc
     1501 gcctgggaca gcgtggtccg cagggcgtgg gaaaggtcgt ggctgatgtt gtcggagccc
     1561 agggcgagct ccttcgtgaa gcgtacgctg ccttcggcgt acacggccaa gcccgtggtt
     1621 tgccccccca ggtccaccaa caggcaaccc aggccttttt cctcggcgct cacgacggtg
     1681 tcgcccaccg ccaaaaggcc gtagatcggt tcctccactt cgaagcccgc ccgggcgatg
     1741 gctttccaca cgttgttcaa atggctttgg ctggcggtca cgatgtggac gtcgacttcc
     1801 aggaggctgg cttccatgcc caccgggttg ggaacgccgc tttgccggtc gaggatgaaa
     1861 tcctggggaa tcacgtggac gatttcgcga tccggcgaga tgggcaccgc tttggtgttt
     1921 tccaccacct ggtcccggtc cgagacggtg atttccttgt cggtccgggc gatgttcatg
     1981 gcgccgtggt ggttgaaggt ttgaatgtgg gcgccgcgga cgccgatgag cacccggcgg
     2041 gaggccccgg cgagtccggc catttcctcg gcggcttcga cggcccgggt cacggcgcgg
     2101 gccgtttcgt cgatgttgat caccacgccg cccttgagtc cggcgcaggg ctgccgggcg
     2161 gcgccccgga tttccggaac gtcggcgtcg ggcgcttgtt cggcgaccac ggcgaccact
     2221 tggccgctcc ccacatcgag accggtgatg agatcggatt taggcatcgt attccctcag
     2281 gcgcgacggc ggggcggggc ggcggccgcg cgcggtttgg ccgccacgcg ccggtcgtcc
     2341 acaaagcgaa tgtattcgaa tcccccggcg ccctccggcg ccgccagcac acgcgacagc
     2401 cgccgggctt tcgggcccag
//
LOCUS       DAOQLS010000300         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27356_length_2416_cov_4.216434, whole genome shotgun sequence.
ACCESSION   DAOQLS010000300 DAOQLS010000000
VERSION     DAOQLS010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27356_length_2416_cov_4.216434"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK362_06345"
     CDS             <1..572
                     /locus_tag="PK362_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC74485.1"
                     /translation="LDYAVEDMIDLIQESLEGTDRVVKIVKDLKNFSRVDEAEHQHAN
                     INDCLDSTINIVWNELKYKAQLTKEYGALPRIKCFPRLLNQVFMNLLVNASHAIEKQG
                     VITVKTWQDNGSIVASVSDTGCGIPEPIRKKIFEPFFTTKEAGKGTGLGLSIAYDIVA
                     RHKGEITVDSEVGRGTTFTVRVPALEPQS"
     gene            569..997
                     /locus_tag="PK362_06350"
     CDS             569..997
                     /locus_tag="PK362_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74486.1"
                     /translation="MNPIRLLCVDDERNVLRALQRVFRSERFQLTTAQSGPEALTLLE
                     KGPVPEVVLADYRMPGMNGVELLEQVHERWPDTLRIILSGYAEATAVEEALRLGKVFR
                     FFAKPWDDRELISAIEQAVEHPVCRLDWRRGRSGVPPPLP"
     gene            994..>2416
                     /locus_tag="PK362_06355"
     CDS             994..>2416
                     /locus_tag="PK362_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC74487.1"
                     /translation="MTTGPATLLFVDDETNTLNALKRVFADQGHRVLLANGPAQALEV
                     LSREPVDLLCSDQNMPGRTGLDLLKEVRERWPHIKRLMLTVVTDAAVMREAVESGAVY
                     KYLNKPWDDDDLKMTVRRALEHGHLESAHDRLRDRTSAQEQMVEKIDRLWDRPRGTLG
                     YVLEKAGLATADQMAAAGRERRAPETLIETLARMDLVTEAQVLRAVAEAQHIPTIELE
                     GVALAADAVKMVPREICEKGRLAPVRVENDRLVVAFADPTDVVKIENLAIVTGLPVTP
                     LAATPSGILKSLDRFFGDSPAYTSLQDFEKQVQYDHGGRFDLVVEEEEPNALDKLFTG
                     TDEASVVRLVNAIVFEGLKSRASDIHIEPKTRVSLVRYRVDGMLQTRLKIPAPLHAPT
                     MSRLKILAKLDIAERRRPQDGRLTLKAGPRLIDVRLSTMPTVEGEKMVLRILDTSGAV
                     KKVADVGFAAGDLLRLSPLLRRPQ"
BASE COUNT          484 a          776 c          743 g          413 t
ORIGIN      
        1 agctggatta cgccgtggag gacatgatcg atctcattca ggagtccctg gaaggcaccg
       61 accgggtcgt caaaatcgtc aaggatttga agaacttttc ccgggtcgac gaggccgaac
      121 accaacacgc caacatcaat gactgtctgg acagcaccat caacattgtc tggaacgaat
      181 tgaaatacaa agcccagttg accaaggagt acggggccct gccccgcatc aaatgtttcc
      241 cgcgcctgtt gaaccaggtg ttcatgaact tgctggtcaa cgcctcccac gccattgaaa
      301 aacaaggggt gatcacggtc aaaacctggc aggacaacgg ctccatcgtg gcctccgttt
      361 ccgacaccgg ctgcggcatt ccggaaccga ttcgaaaaaa gatcttcgag ccgtttttta
      421 cgaccaaaga ggccggcaag ggaacgggcc tgggcctctc catcgcctac gacatcgtcg
      481 cccggcacaa aggcgagatc acggtggaca gcgaagtggg ccgggggacc accttcaccg
      541 tacgcgttcc ggccctggag ccgcaatcgt gaatccgatc cgcctccttt gcgtggacga
      601 cgaacggaac gtgttgcgcg cccttcagcg cgtgtttcgc tccgagcgat ttcaattgac
      661 gacggcccaa tccggacccg aggccctgac cctcctggaa aaggggccgg tccccgaagt
      721 ggtcttggcc gattaccgca tgccggggat gaacggggtg gaattgctgg aacaggttca
      781 cgaacggtgg cccgacaccc tgcgcatcat cctgtccggc tacgccgaag ccacggcggt
      841 ggaagaggcc cttcggctgg gaaaagtctt tcgtttcttc gccaaacctt gggacgaccg
      901 ggagttgatt tccgccattg aacaggcggt ggagcacccg gtgtgccgcc tggattggcg
      961 ccggggccgt tcgggcgttc cgccccccct gccatgacga ccggccccgc gaccctgctg
     1021 ttcgtggacg acgagaccaa cacgctgaac gccctcaagc gggtcttcgc cgaccaaggc
     1081 caccgggtgc tcctggccaa cggcccggcc caggcgctgg aggttctctc ccgggagccg
     1141 gtggacctcc tctgcagcga tcagaacatg ccgggccgga cgggcttgga cctcctaaag
     1201 gaagttcggg agcgctggcc ccacatcaag cgtctgatgc tgaccgtcgt gaccgacgcg
     1261 gcggtcatgc gggaggccgt ggagagcggg gcggtttaca aatacctcaa caaaccctgg
     1321 gacgacgatg atttgaaaat gaccgtgcga agggccctgg aacacggcca tctcgaatcg
     1381 gcccacgacc gtctgcggga ccgaacctcg gcccaggaac aaatggtcga gaaaatcgac
     1441 cggctctggg accgcccccg gggaaccctg ggctacgttc tcgagaaagc gggtctcgcc
     1501 acggccgacc agatggcggc ggcgggccgc gagcggcggg cgcccgaaac cctcatcgaa
     1561 accctcgcgc ggatggattt ggtgaccgag gcccaggttc ttcgggcggt ggccgaggcc
     1621 caacacatac cgaccatcga actggagggc gtggccctgg ccgccgacgc cgtgaaaatg
     1681 gtcccccggg aaatttgtga aaaaggtcgt ttggccccgg tccgcgtgga aaacgaccgg
     1741 ttggttgtcg ccttcgccga tccgacggac gtcgtcaaaa tcgaaaacct ggccatcgtg
     1801 accgggctcc ccgtgacgcc cctcgcggcc acgccgtcgg gcattctcaa aagcctcgat
     1861 cggtttttcg gcgattcgcc ggcctacacg tcccttcagg attttgaaaa gcaggttcag
     1921 tacgaccacg gcggtcggtt cgacttggtg gtcgaggagg aggagccgaa cgccctggac
     1981 aaactgttca ccggaaccga cgaggcttcc gtggtccgcc tggtgaacgc cattgtcttc
     2041 gagggtctca aaagccgggc ctccgacatc cacatcgagc ccaaaacccg ggtgtccctg
     2101 gttcgctacc gcgtggacgg catgcttcag acgcgcttaa aaatcccggc cccccttcac
     2161 gcgcccacca tgtcgcgcct caaaatcctg gccaaactgg acatcgccga gcggcgccgt
     2221 ccccaggacg gacggttgac gctcaaagcc ggtccgcgtc tcatcgacgt ccggttgtcc
     2281 accatgccga cggtggaagg ggaaaagatg gtcctgcgta ttctggacac gtcgggggcg
     2341 gtgaaaaagg tggccgacgt ggggttcgcc gcgggagacc tcctgcgcct ttcccccctc
     2401 ctgcgccgac cccagg
//
LOCUS       DAOQLS010000301         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27420_length_2413_cov_4.930025, whole genome shotgun sequence.
ACCESSION   DAOQLS010000301 DAOQLS010000000
VERSION     DAOQLS010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27420_length_2413_cov_4.930025"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PK362_06360"
     CDS             complement(<1..789)
                     /locus_tag="PK362_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNC74488.1"
                     /translation="MKTRTEKDSLGPKEVPADALYGIQTLRAVENFPVSGWRFSRSFI
                     RALALIKKSAAEVNLKRGDLDPKVGGAIVQAAGEVADGKWDDQFPVDIFQTGSGTSTN
                     MNANEVIASRANELLGGQRGAKSPVHPNDHVNKGQSSNDVIPTTIHVAALEALERDTI
                     PALEGLRAALEEKAKAFDGVLKIGRTHLQDAVPMRLGQEFGGYASQIAHGIQRLKNVR
                     PHLAELALGGTAVGTGLNADPEFIDVVIRRLAEVLGLPFVGAPSR"
     gene            890..>2413
                     /locus_tag="PK362_06365"
     CDS             890..>2413
                     /locus_tag="PK362_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNC74489.1"
                     /translation="MTTPAGRSRVLEVAMELALTPELLQAIEDQFGLEAAQAAAMSTS
                     VGGIGPLLRERVIAQSEQGVDVVGVSLLYNSVWNQGLHAWNHLMLDRRPVAPSLRAVL
                     QDTGIHLSLPLFDGSTAEVKVWRAPYGQSFVYFLDCPSIADVVYPGGEDAPPKTPDPE
                     AWTERQRLKHSWIVGRGALALAKALNFSPDVVVLSETPTLFAHPTLVNDDLSRDPLFA
                     HTRTVFNDHTPLEYAHPIWPEDMLRLTRMNAALYGPFVRNGRVDITQLLVACSDGVFG
                     VARKHADVMRAMPSLSAYAAKIQYITNGVSQALWQHPVFRLADKIGDAELLAAKDKLR
                     EEFLEWLWRRALLWPHWARAVRGRPIVLWTRRITSYKRLDILDKIFDRPDWRQRFLDS
                     GVVIIVGGRVYQRDNVSEKMLYNLVEDLNGDETLGERVVFLHNYNVWEAPRLFWGGDA
                     SIMLSDDGREASATGFMKAQMNGAAIIANPDGAVPEFVFGDDSAGKPVNGFTVRYRDG
                     "
BASE COUNT          408 a          782 c          761 g          462 t
ORIGIN      
        1 ccggctgggc gcgcccacga agggcaaccc caacacctcc gccaggcggc ggatcacgac
       61 gtcgatgaat tccgggtcgg cgttgaggcc cgtcccgacg gccgttcccc cgagcgccag
      121 ttccgccagg tggggccgga cgttcttcaa ccgctgaatc ccgtgggcga tttggctggc
      181 gtagccgccg aattcctggc cgagccgcat cgggacggcg tcctgcaaat gggtccgtcc
      241 gattttcaat acgccgtcga aggccttggc tttttcctcc agggcggccc gcaggccctc
      301 caaggcggga atcgtgtcgc gctccagggc ctccagggcc gccacgtgaa tcgtggtcgg
      361 gatcacgtcg ttggaggact ggcctttgtt gacgtggtcg ttgggatgga ccggggattt
      421 ggccccccgt tgtccgccca gcagctcgtt ggcccggctg gcgatgacct cgttggcgtt
      481 catgttggtg gaggtgccgg aaccggtttg gaagatgtcc acgggaaatt gatcgtccca
      541 tttcccgtcc gccacctccc cggcggcttg aacgatggcg ccgcccacct tcggatcgag
      601 gtcgccgcgc ttcaggttga cttcggcggc ggacttcttg atgagcgcga gggcccgaat
      661 aaaggagcgg gagaagcgcc atccgctcac gggaaaattt tcgaccgcgc gcagggtttg
      721 aataccgtac aacgcgtcgg cgggaacttc tttggggccc agggaatctt tctcggtgcg
      781 ggttttcatg gcggcttcct cctgaggaac ggcgagggtc cattttaaca attcggaaga
      841 ccgcgttggt tttttgacag gttcgtcggg gaagagatat catggcccca tgacgacccc
      901 cgccggacga tcccgcgttc tggaagtggc catggaattg gccctgaccc cggagctgct
      961 ccaggccatc gaagaccaat tcggcctgga agccgcccag gccgccgcca tgtccacgtc
     1021 ggtgggcggc atcggtccgc tgttgcggga acgcgtcatc gcccaatcgg aacagggcgt
     1081 ggacgtcgtg ggcgtttccc tcctctacaa ttccgtttgg aaccagggct tgcacgcctg
     1141 gaaccacctg atgctcgacc ggcggcccgt ggccccctct ttaagggccg tcctgcagga
     1201 cacggggatt cacctttccc tccccctgtt cgacgggtcc accgcggagg tcaaagtctg
     1261 gcgggccccg tacggacaat ctttcgttta ctttctcgac tgcccctcga tcgccgacgt
     1321 ggtgtacccc gggggcgaag acgctccgcc caaaaccccc gaccccgaag cgtggacgga
     1381 gcggcaacga ctcaaacaca gttggatcgt cggccgcggc gccctggcct tggccaaggc
     1441 cctgaatttt tcgccggacg tcgtcgtgct gagcgaaaca cccacgttgt tcgcccaccc
     1501 caccttggtg aacgacgacc tgagccggga cccgctcttc gctcataccc ggacggtgtt
     1561 caacgaccac acgcccctgg aatacgccca cccgatttgg cccgaggaca tgctgcgcct
     1621 gacccggatg aacgccgccc tttacggtcc cttcgtccga aacggccggg tggacatcac
     1681 gcaacttctg gtcgcctgtt ccgacggcgt gttcggcgtg gcccgaaaac acgccgacgt
     1741 catgcgggcc atgccttccc tgtcggccta cgccgccaaa attcaataca tcaccaacgg
     1801 cgtgagccag gccctgtggc aacacccggt gttccgcttg gcggataaaa tcggcgacgc
     1861 cgagctgttg gccgccaagg acaagcttcg ggaagaattc ctggaatggt tgtggcggcg
     1921 ggccctcctg tggccccatt gggcccgggc ggtccggggc cggcccatcg ttttgtggac
     1981 ccggcgcatc accagttaca agcggctcga tattttggac aaaattttcg accggcccga
     2041 ttggcgccaa cggtttctcg attcgggcgt cgtgatcatc gtggggggac gtgtttacca
     2101 gcgggacaac gtgtccgaga agatgctcta caacctggtg gaggatttaa acggggacga
     2161 gaccctgggc gagcgcgtcg tgtttcttca caactacaac gtctgggagg cgccccgtct
     2221 cttttggggc ggcgacgcct ccatcatgct ctcggacgac ggccgggaag cctccgcgac
     2281 cgggttcatg aaagcccaaa tgaacggcgc ggcgatcatc gccaaccccg acggcgccgt
     2341 cccggaattc gttttcgggg acgacagcgc cggaaaaccg gtcaacgggt ttacggtgcg
     2401 ctaccgggac ggc
//
LOCUS       DAOQLS010000302         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27546_length_2407_cov_5.611820, whole genome shotgun sequence.
ACCESSION   DAOQLS010000302 DAOQLS010000000
VERSION     DAOQLS010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27546_length_2407_cov_5.611820"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /locus_tag="PK362_06370"
     CDS             complement(<1..2353)
                     /locus_tag="PK362_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74490.1"
                     /translation="MTTEIPSSPPPPEDKEKDLVGKIVGKEEAFARGVGVSGAQIPEL
                     AYGATALVADSAEKLFGSGTASRVKDWALRGYQRASKVSEEVHGDEVKSVSESWKEAK
                     KGNLDALGDWISYWTGYGAGQLGESAAVAFLGGLVGSVVPGPGTAAVAVAGVTAKTAT
                     KGVIRGALERLVERQALKLTAEGVVAAEAKKLAVKQIATAIGAASAIGAYAVVQEGGS
                     IYPDAVKKAEEDGRKLNGYDLGRAWGTTLAAAGADGLEDMLGLGFLTGKVRIPGRLGK
                     VARIVGTAASGSLAGSGTEAIQTAVERVGAGRKPFDQEGIQDIIESAAAGAIPGLGAG
                     VGGAIISGQPRVENGGLAATAEASPELLNYLDRVEKINRAQVSDVTESQQREDVTKGQ
                     ARAEFIGYQEGLPGDTPIPLYNVMGESPRSLSTVGQDELKNLGIEVPKTPTFEGWKAQ
                     GDVTEGQMAAKSLIEKLASANKISDAIAVEFVKHITPTGENFYKSYGEEFSPEKFRVL
                     GATIPQYTQNGLNFAIKLAQGAKESTGAHEMFHAMKQMGFFSQDEMGVLERRFGSEEK
                     IADALGEYATRRTGAEAQSRIKAIFDRFLEFVKRVGSMLVGRGWTKAEDIFESVVKGR
                     PGKRAFGPDGSMRFSAPPQGPIFYSQLQRVLQEKMPNSAPVEMVRALIDPAKGNVKAE
                     EVEWSGINDFLQDKEKVSKAELLDFLKQNQVEVKEVVKGIEGKFFIKGKDGERITDAV
                     FETKNEAQAWVDKNRPENFYAIAQDIGQGTAAKFSKYQLPGGEN"
BASE COUNT          439 a          782 c          614 g          572 t
ORIGIN      
        1 agttctcgcc gccggggagt tggtatttgg agaattttgc ggctgtccct tgtcctatgt
       61 cctgggcgat tgcatagaaa ttttcagggc gatttttgtc cacccaagcc tgcgcctcgt
      121 ttttagtttc aaatactgcg tccgttattc tttcaccatc ttttccttta ataaaaaact
      181 taccctcaat tcctttcacc acttctttca cttccacctg attctgtttc aggaagtcca
      241 gcagttccgc cttggacacc ttctctttgt cctggaggaa atcattgatc cccgaccact
      301 ccacttcctc agccttcacg ttccccttgg ccgggtcgat gagggcgcgc accatctcga
      361 cgggggccga gttgggcatc ttttcttgga ggactctctg gagttgtgag taaaaaatcg
      421 gcccctgcgg tggcgcactg aaccgcatcg atccatcggg accaaaagct cttttccctg
      481 gacggccttt gacaacagac tcgaagatat cctccgcctt tgtccagccc ctgccgacca
      541 acatgctccc gacccgcttc acgaattcga ggaagcggtc gaaaatggcc ttaattcggg
      601 actgagcctc cgctcccgtc ctccgggtcg cgtattcacc gagagcgtcc gcgatctttt
      661 cctcgctccc gaaccgacgc tcaagcacgc ccatctcgtc ctggctgaaa aagcccatct
      721 gcttcatggc gtggaacatc tcgtgggcgc cggtcgattc cttggccccc tgagcgagtt
      781 tgatcgcaaa atttagcccg ttctgcgtgt attgcgggat cgtggccccg aggactcgga
      841 acttctccgg cgagaactcc tcgccgtagg acttgtagaa gttctctccc gttggcgtga
      901 tgtgcttcac gaactcaacg gcgatggcat cagagatttt gttcgcggac gccagcttct
      961 cgatcaacga cttcgccgcc atctgtcctt cggtgacatc cccctgggct ttccatccct
     1021 caaacgtggg cgtcttggga acctcaatgc cgaggttttt aagctcatcc tgcccaaccg
     1081 ttgaaagaga tcgcgggctc tcgcccatga cgttgtagag cgggatcggc gtgtccccag
     1141 gcaagccctc ctggtagccg atgaactcgg cccgggcctg gcccttcgtc acgtcctccc
     1201 tctgttgcga ctccgttaca tcagacactt gcgcccggtt tatcttctcc acccggtcga
     1261 ggtagttcaa aagttctgga gacgcctctg cggtggccgc gaggcctccg ttttctacgc
     1321 gaggctggcc ggatatgatc gcaccaccca caccagcgcc aaggccaggg attgcgccag
     1381 cggcggcgga ttcgatgatg tcctgaatcc cttcctgatc aaacggttta cgcccggccc
     1441 ctactcgctc aacggcggtt tggatggctt cggtcccact ccccgcaagg ctaccggaag
     1501 ccgccgttcc aacgattctg gcgacttttc caagacgccc agggattctg accttgccag
     1561 tcaagaaacc gagaccaagc atgtcctcga gcccatctgc tccggcggcg gccaaggtgg
     1621 ttccccaagc gcggccgaga tcgtatccgt tcagtttcct tccgtcctcc tcagccttct
     1681 tcaccgcgtc cgggtaaatg ctaccgccct cttgaaccac cgcatacgcg ccgatggcgg
     1741 aagcggcacc gatggcggtg gcgatctgtt tgacagcgag tttcttggct tcggcggcga
     1801 caacgccttc ggctgttaat ttaagcgcct gacgctccac cagccgttcc agggctccgc
     1861 gaatgacacc cttggtggct gtttttgccg tgacgccggc gacggcaacc gcagcggtcc
     1921 ctggcccagg gacaaccgag ccgacgagcc cgccaagaaa agccacggcc gcagattctc
     1981 caagctgacc agccccgtag cctgtccagt atgaaatcca atccccaagt gcgtcaaggt
     2041 tccctttttt ggcttctttc catgactcgg aaacgctctt gacctcgtcg ccgtggactt
     2101 cctcggacac cttggacgct ctctgatacc cgcgcaaagc ccaatccttg actctcgatg
     2161 cggtgccgct gccaaagagc ttctccgccg agtccgcgac caaagccgtg gccccatagg
     2221 ccagttccgg gatctgggca ccagaaactc cgacgcccct ggcaaaggct tcttccttcc
     2281 caacgatttt cccaacgaga tctttttcct tatcttcggg aggaggcgga gaagaaggta
     2341 tttctgttgt cataattcgg cgtatggtct gaccgtgacg gccaggatat tcaacgagtg
     2401 tcggctt
//
LOCUS       DAOQLS010000303         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27555_length_2407_cov_3.738946, whole genome shotgun sequence.
ACCESSION   DAOQLS010000303 DAOQLS010000000
VERSION     DAOQLS010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27555_length_2407_cov_3.738946"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2407
                     /locus_tag="PK362_06375"
     CDS             <1..>2407
                     /locus_tag="PK362_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNC74491.1"
                     /translation="DAGQTLADFDNTAPELFAFKDEKGTVLNASTAYADGEKGKHLGI
                     RYGIVPGGFGGAGLALKGFNASGYRYLGLDIRGDAGGESFDVGLRDAKGQERKRSIAA
                     YTDVAKTWRRVLIPLGDFGGVTLSALDNISFGFAEKKKGRIYIDNIGFEGAAAGSDGA
                     LVNKVVVDGFDRTNPTQAYRVFTGDQSSLSLTSSRVLFDGDYSMEMQYQFSTNRPWGT
                     WVSAQRTPAEPLNWTGIDEIKMWVKGDGSENYLRFRFTESDGEIWEYVDKTALASTRW
                     TLVGMPIKAFKIVGQPPKGTPPDLAGVKAYEIALMSPTSSQTSGAQVTAGRIQVDLLY
                     VTGERLSSGAIVPAAAPQPGAPAGAAIPSGRIPGLAAGNVDFSLQAYTEYFYTPEEKS
                     AVNHLAKLTTTGKLGNYSARVEFGSTSQEFGQASTFVGSSVTATTNRFPGLEMPSFQV
                     FITNLHPAVSLVTLGNLFIDYSPDLFAPVFGFKGIEVQGDVDRLNYEGFVIKHSLNSF
                     TGGARGVYYLPNLKLSAYAVYWEQNGRQSNASAASGGSLQPAANSAVLKLERLAQDMT
                     YHLQADARTWNDRLRLVSLYGYNTYRQNATADYSDPFNPVFSQFVDPAYQAGGHLWRN
                     RVELYGAGDFLGWRGLDLAYGYRDIGTGYKPHFRQVPVFYDDTDSDQWGHNVRAVQRR
                     GGWTLSAEYDAMRRHSDAAYFRHKFLWGLGHYGYRGMDVSLTQDYRRETYKFTSDRSA
                     FTTDKNEKVIGTELYVRAQLSSRVAGWVKPRQERIWHPATNGTFTSDSLQARLEYYVA
                     NNAK"
BASE COUNT          469 a          772 c          721 g          445 t
ORIGIN      
        1 ggacgccggt caaaccctcg cggactttga caacaccgcc ccggaacttt tcgcgttcaa
       61 agacgagaag ggaaccgtgc tgaacgcgag caccgcctac gccgacggcg agaagggcaa
      121 gcacctggga attcgctacg gcatcgttcc cggcggtttc gggggcgcgg gcctggcgct
      181 taaaggcttc aacgcatcgg gataccgtta tttgggtctc gacatccggg gggacgcggg
      241 cggggaatcc ttcgacgtgg gtctgcggga cgccaaaggc caggagcgga agcggtccat
      301 cgccgcttac accgacgtgg ccaaaacttg gcggcgggtg ctcattcctc tgggggattt
      361 cggcggggtg acgctgtccg ccctggacaa tatcagtttt gggtttgccg agaagaaaaa
      421 ggggcggatc tacatcgaca acattggttt tgaaggcgcc gccgcgggat cggacggggc
      481 cctggtgaat aaagtggtcg tggacggttt tgaccgcacc aacccgaccc aggcttaccg
      541 ggtgttcacc ggggaccagt cctccctttc cttgacgtcg tcgcgggtcc tcttcgacgg
      601 cgactattcc atggagatgc aataccaatt ctcgaccaac cgcccctggg gcacctgggt
      661 ttccgcccag cggacgcccg ccgagccgct caactggacc ggcatcgacg agatcaaaat
      721 gtgggtcaag ggcgacggtt ccgaaaacta tcttcgtttc cgttttaccg aatccgacgg
      781 ggaaatttgg gaatacgtgg acaaaacggc cctggcgtcc acccggtgga ctctggtggg
      841 catgccgatc aaggccttca aaatcgtcgg ccaaccgccc aagggcactc cgccggattt
      901 ggccggggtg aaggcctacg aaatcgcctt gatgagtccg acctcctccc agacgtcggg
      961 ggctcaagtc accgcgggac gcatccaggt cgacctcctt tacgtgacgg gagagcgtct
     1021 ctcttccggc gcgatcgtgc ccgccgccgc gccccaaccg ggcgcccccg ccggagcggc
     1081 gatcccgtcc ggccgaattc ccggattggc cgccggcaac gtggacttca gccttcaagc
     1141 ctacaccgaa tatttctaca cgccggaaga aaaaagcgcg gtgaatcacc tggccaaact
     1201 caccacgacc ggaaaattgg ggaactacag cgcccgggtg gagttcgggt ccacgtccca
     1261 ggaatttggc caagcttcga cctttgtcgg gtcttcggtg acggccacca ccaaccgctt
     1321 tcccggcttg gaaatgccca gcttccaggt cttcatcacc aaccttcacc ccgcggtctc
     1381 cctggtgacc ctcggaaacc tcttcatcga ttattcgccg gatctcttcg ccccggtttt
     1441 cggttttaaa ggcatcgaag tgcagggcga cgtggaccgc ctaaactacg aagggttcgt
     1501 gatcaaacac tcccttaatt cgtttaccgg cggggcccgc ggggtttatt atctgcccaa
     1561 tctcaagttg tcggcctacg cggtctactg ggagcagaac ggtcggcaat ccaacgccag
     1621 cgcggcgtcc ggcgggtccc tgcaaccggc cgccaacagt gccgtgttga aattggagcg
     1681 cctggcccag gacatgacgt accacctcca ggcggatgcc cggacctgga acgaccgcct
     1741 tcgactggtc tccctttacg gctacaacac ctatcgccaa aacgccaccg ccgattacag
     1801 cgatcccttc aacccggtgt tttcccagtt cgtcgacccg gcctatcaag cgggggggca
     1861 cctttggcgg aaccgggtgg aactctacgg cgccggggat ttcctcggct ggcgggggtt
     1921 ggacctggcg tacggatacc gggacatcgg cacgggctac aagccccatt tccggcaggt
     1981 gcccgtattc tacgacgaca cggattcgga ccaatggggc cacaacgtcc gggccgttca
     2041 gcggcgcggg gggtggacgc tctccgccga atacgacgcc atgcgtcgcc actccgacgc
     2101 ggcctatttt cggcacaaat ttctctgggg cctgggtcac tacggctacc gggggatgga
     2161 cgtctccctg acccaggatt accggcggga aacctacaag ttcacgagcg accgttccgc
     2221 ctttaccacc gacaaaaatg aaaaagtgat cggcaccgaa ctttacgtcc gcgcccaact
     2281 gtcgtcccgg gtggccggct gggtcaagcc ccggcaggag cgcatctggc acccggcgac
     2341 gaacgggacg ttcacttccg acagccttca ggcccgcttg gaatattacg tggccaacaa
     2401 cgcgaag
//
LOCUS       DAOQLS010000304         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27614_length_2404_cov_4.093657, whole genome shotgun sequence.
ACCESSION   DAOQLS010000304 DAOQLS010000000
VERSION     DAOQLS010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27614_length_2404_cov_4.093657"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..905
                     /locus_tag="PK362_06380"
     CDS             63..905
                     /locus_tag="PK362_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC74492.1"
                     /translation="MARKIKHPEHVNHERWLVSYADFITLLFAFFTVLYATAQTDQGK
                     VNKLVGAVEKAFTAGIFSAGSDQLMFNDQGTARGNGVMDLGMNLREIETQLRLSIGDE
                     GGGALRLERHADGLTLILQARQYFAPGSATLRSEALEPLRRAARILRLIRHRLRVEGH
                     TASTGGSDPARTNWELSAARGLSVLRWLESQGVSAQRLSVAGYAEYRPLQPNTTAAGR
                     ARNRRVEIVILDEKPSFWRSLKPAAEGSSRPDEGWRPWIAERPAAAPAPTARPPAEAP
                     HVRP"
     gene            892..1434
                     /locus_tag="PK362_06385"
     CDS             892..1434
                     /locus_tag="PK362_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74493.1"
                     /translation="MSVRESHGLEVVAHLPGEIHSLSSVEAERVRSQILDAAVCRAQA
                     SPLDLPLEVPEGLEAFAHRLSQLEERVQRLVDRLDKPAEKPLSPLPVRLRPDGFSPLS
                     SEFTPALREGDWVEIKLVLPPGHDDPLWILGQAVREAAGPAVAFRCVAQDQADHLVRY
                     LLHRQRLDQTQPPFAGASDR"
     gene            1499..>2404
                     /locus_tag="PK362_06390"
     CDS             1499..>2404
                     /locus_tag="PK362_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74494.1"
                     /translation="MLNRRTAVAVVFLFAAPAARAAFSTSTFTASPYASFEYLGQQAK
                     NETDFDNVENDERGNTVDRIRLGLNIGFSDQLRGGVEVVRAPFIAGTPNQDAQFGNPP
                     RSVNTELSNFYLENAFLEMDDILGFDSVRVGRQYIGRPGDFIAYYGPLEDNQLSVRSL
                     QGVSVREQIGLVWGQFAFGTPVDDDAIPQAGAAGDAATNKDDFVDGGDVSLTYGFLNS
                     DGLFKKGWGKFELEAGFYQAVDQNSGLSRDSNKLQIYDTRARFYVPVDGRDAVSLSVE
                     WAVNGGADRGPLFDNLGTLISSGVAR"
BASE COUNT          414 a          793 c          761 g          436 t
ORIGIN      
        1 gttgaaggaa cgcctcaaag gctacttgtc ccacgccgag cgggcgactc tggagaaatg
       61 acgtggcccg aaaaatcaaa catcccgaac acgtcaacca cgagcggtgg ttggtgagtt
      121 acgccgactt catcacgctc cttttcgcct tcttcacggt cctctacgcc accgcccaaa
      181 ccgatcaggg aaaggtcaat aaattggttg gcgccgtgga aaaggccttc acggcgggca
      241 tcttttcggc cggcagcgac cagctgatgt tcaacgacca gggcaccgcc cgcggaaacg
      301 gcgtcatgga tctgggcatg aaccttcggg aaatcgagac ccagctgcgt ctttccatcg
      361 gcgacgaagg cggcggggcc cttcgattgg aacgccacgc cgacgggttg accctgattc
      421 tccaggcccg gcagtacttt gcccccgggt ccgccacgct ccgctccgag gcgctggagc
      481 ccttgcggcg ggccgcgcgc atccttcgcc tgatccgcca ccgccttcgg gtggaagggc
      541 acacggcttc gaccggcgga agcgacccgg cccgaaccaa ttgggagctg tccgcggccc
      601 ggggactgtc ggtcctccgc tggctggagt cccagggcgt ttccgcccag cgcctctccg
      661 tggccggtta cgccgaatac cggccgcttc aacccaacac gacggccgcc ggccgggccc
      721 gcaaccggcg ggtggaaatc gtcattttgg acgagaaacc ttctttttgg agatcgctaa
      781 aaccggcggc cgaagggtcg tcccgtcccg acgaagggtg gcgaccctgg attgccgagc
      841 ggccggccgc ggccccggcg cccaccgcgc ggcccccggc ggaggccccc catgtccgtc
      901 cgtgaatccc acggcctcga ggtggtggct cacctgcccg gggagatcca ttccctatcc
      961 tccgtcgaag ccgagcgggt tcgatcccaa atcctggacg ccgccgtgtg ccgagcccag
     1021 gcctcgccgt tggatttgcc cctggaggtg cccgagggcc tcgaggcctt cgctcaccgc
     1081 ctttcccagc tggaggaacg agttcaacgg ctggtcgatc ggcttgacaa acccgcggaa
     1141 aagccgcttt cgccgctccc ggtccggttg cgtccggacg gattttcccc cctttcttcg
     1201 gaattcaccc cggccttgcg ggaaggcgat tgggtggaaa tcaagttggt gctccccccg
     1261 ggccacgacg atcccctttg gattttgggg caggcggtcc gggaagcggc gggtccggcc
     1321 gtggcgttcc gttgcgtggc ccaggatcag gccgaccacc tcgttcgcta tctgctccac
     1381 cgacaacgcc tggaccaaac gcaaccgccc ttcgccgggg cttcagatcg atgaaaggcg
     1441 gccgatatca caaacgacgc ctcggcgtcg gattcgttgg gatttgggag gaacgcgtat
     1501 gttgaaccgt cgcaccgccg tcgccgtcgt ttttttgttc gccgcccccg cggcgcgagc
     1561 cgccttttcc acgtccacct tcacggcgtc gccttacgcg tccttcgaat acctggggca
     1621 acaggccaag aacgaaacgg atttcgacaa cgtggaaaac gacgagcggg gcaacaccgt
     1681 ggaccgcatc cggctcgggt tgaacatcgg gttttcggac cagcttcgcg gcggcgtgga
     1741 agtcgtccgg gcgcccttta tcgccggcac ccccaatcag gacgcccaat tcggcaatcc
     1801 cccgcggtcc gtcaacacgg agttgagcaa tttctatttg gaaaacgcct tcctcgaaat
     1861 ggacgacatc ctggggttcg attcggtgcg cgtggggcgg caatacatcg gccgtccggg
     1921 ggacttcatc gcctattacg gccccctcga ggacaaccag ctgtccgtcc gctcgttgca
     1981 gggggtgtcg gtgcgggaac agatcggcct ggtgtggggg cagtttgcct tcggcacacc
     2041 ggtcgacgac gatgcgattc cccaggcggg cgccgccggc gacgccgcca cgaacaagga
     2101 cgatttcgtg gacggcggtg acgtgagctt aacctacgga tttttaaact ccgacgggtt
     2161 gttcaaaaaa ggctggggca agttcgagct ggaggcgggc ttctaccagg ccgtggacca
     2221 aaattccgga ctgtcccggg actccaacaa gctccaaatt tacgacaccc gcgcccggtt
     2281 ttacgtgccg gtggacgggc gggacgccgt gtccctctcg gtggaatggg cggtcaacgg
     2341 cggggcggac cggggtccgt tgtttgacaa tttgggaacg ttgatcagca gcggcgtcgc
     2401 ccgg
//
LOCUS       DAOQLS010000305         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27720_length_2399_cov_3.208618, whole genome shotgun sequence.
ACCESSION   DAOQLS010000305 DAOQLS010000000
VERSION     DAOQLS010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27720_length_2399_cov_3.208618"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2052)
                     /locus_tag="PK362_06395"
     CDS             complement(<1..2052)
                     /locus_tag="PK362_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC74495.1"
                     /translation="MALPGRPGAQRPLVLIGFALHLFAPILFFTDRTQNPYLIQIGLI
                     HAGVYLALLVAAYGIFRARQWEWAGTPLDGPVLLLGVVSLLSWGWAVARGGALSASLY
                     HEGSRGVVFLWGNGLGVFFLSTQTAFEWRNRKGPEVMMAVGLAAAAYGLVQYAGIDPL
                     WPHPVNPFTGRPISTFGNPNFLSTALVLLIPLGLWKMMVSERRVAAWTGGGVALVFSV
                     ALLCTMTRSSYVGAGVAFLVFAVGAWDLVRSRPRRLAAWIVLLVFALIFWPGGRLASE
                     APTPMERVVELWNGVVGQKIYASWHQRRLIWSCAIDMWKSRPWTGTGWGTFEVFFPFF
                     QGRYLSDPLFRPFRTHANNAHQILLEFGSQTGLLGVGILFWLGVAAFRLQRARRAWRA
                     DPRWFSAALWAGLWGALADNFFGNVSLFFAVPCFLFCWMAGQWAVGETPVIRRGRLNS
                     VGVAGLSLGLIAAAVGLGREWRLWRAAEGHLRALRLLQAGATARAEESLSESRRWYVW
                     DVHSAYERGNLYARRARWAAGNGLSVEKEENAERAIAAYGEALSANAGYDEIYFNRAG
                     FRADRGDVPGAVRDYRWALLINPLNPDAYRGLAALWERDEPPGGGLRALYESAVFYFP
                     SRSDFWFGLSRARERAGDVEGARRAAAEGVRMDLDSSEGWATLRRLDPVGGPEDLKAA
                     PD"
     gene            complement(2060..>2399)
                     /locus_tag="PK362_06400"
     CDS             complement(2060..>2399)
                     /locus_tag="PK362_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74496.1"
                     /translation="VGRIYHDRNAETYSNLGRLHYILLVNQNPGVSWLPSNSPEFKKA
                     EGYYLKAMEEARLSGREDEVGFEPTKSLAVLYSRSKSGDQAKSLWLKLRAWNPDDPDV
                     RRVFTPPPSS"
BASE COUNT          561 a          803 c          692 g          343 t
ORIGIN      
        1 atcgggcgcg gctttcagat cctccggccc gcccaccgga tccaggcggc ggagagtcgc
       61 ccacccctcc gaagaatcca aatccattcg gacgccttcg gcggccgccc gtcgtgctcc
      121 ttccacgtcg cccgctcttt cgcgcgcgcg actcaatccg aaccaaaagt cggatcgcga
      181 cggaaagtaa aacacggcgc tttcgtacaa cgcgcgcagc cccccgccgg gcggttcgtc
      241 gcgctcccac agggccgcca gaccccggta ggcgtcgggg ttgaggggat tgatgagcag
      301 cgcccatcgg taatcgcgca ccgcgccggg cacgtcccct cgatcggctc gaaaacccgc
      361 ccgattgaaa taaatctcgt cataccccgc gttggccgac agggcctccc cgtaggccgc
      421 gatcgcgcgc tccgcgttct cctccttttc aaccgagaga ccgtttcccg ccgcccaccg
      481 ggcccgacgc gcatagagat ttcctctctc gtaagcgctg tgaacgtccc aaacatacca
      541 tcggcgagat tcggacaggg attcctccgc ccgggccgtc gctccggcct gaagcagtcg
      601 gagagcccga aggtggcctt cggcggcccg ccaaaggcgc cattcccgcc ccagacccac
      661 cgccgccgca atcaatccca acgacaaacc ggcgacgccc acggagttga gtcgcccccg
      721 acggatcacg ggcgtttccc caacggccca ttgaccggcc atccaacaaa acaggaagca
      781 gggcacggca aaaaacaacg agacgttgcc gaaaaaatta tccgccaacg ccccccacaa
      841 cccggcccag agggcggcgc tgaaccaacg cggatcggcc cgccatgccc gccgggcgcg
      901 ttgaagacgg aaggccgcca cgccaagcca aaaaagaatt cccaccccca gcagaccggt
      961 ttgtgaaccg aattccagaa ggatttggtg ggcgttgttg gcgtgggttc gaaagggccg
     1021 gaacagggga tccgaaaggt aacgcccctg gaaaaatgga aaaaaaacct cgaaggtccc
     1081 ccaaccggtc cccgtccagg ggcgggattt ccacatatcg atcgcgcagg accagatcag
     1141 gcgccgttga tgccacgaag cgtagatttt ctggccgacc acgccattcc aaagctcgac
     1201 gacgcgctcc atgggagtcg gcgcctccga agccaatcga ccgccgggcc aaaaaatcaa
     1261 ggcgaacacc agcaaaacga tccaagccgc caggcgccgg ggacgggacc gaaccaaatc
     1321 ccacgcgccg accgcaaaaa ccagaaaagc cactcccgct cccacatacg acgaccgggt
     1381 catggtgcac agcaaggcca ccgaaaagac gagggcgacg cctccccccg tccacgccgc
     1441 gactcttcgc tcggaaacca tcattttcca aagacccagc ggaatcaaca gcacgagcgc
     1501 ggtggataaa aaattcggat ttccaaaggt cgaaatgggc cgcccggtaa aaggattgac
     1561 ggggtggggc cacaggggat caatgcccgc gtattgaacc aacccgtagg ccgcggccgc
     1621 caagccgacg gccatcatga cttcgggccc ctttcgattt cgccattcaa aggccgtttg
     1681 cgtcgacaaa aagaagacgc ccaggccgtt cccccaaagg aacacgacgc cgcgagatcc
     1741 ttcgtggtac aaagaagcgg agagagcccc cccccgagcc acggcccacc cccacgacag
     1801 cagcgacacg acgcccagaa gaagaacggg gccgtccaga ggcgttcctg cccattccca
     1861 ttgccgggcg cggaaaattc cataggccgc gacgagaagg gcgagataga caccggcgtg
     1921 aatcaagccg atctgtatga gatacgggtt ctgggtccgg tccgtgaaga agagaatcgg
     1981 ggcgaacaga tggagggcga aaccgatcaa caccaggggg cgctgggcgc cggggcgacc
     2041 cgggagggcc atgacgtttc taggaggaag gcggcggggt gaagacccgg cgcacgtccg
     2101 gatcatcggg gttccaagcc cggagcttca gccagaggga cttggcttga tcgccggatt
     2161 tcgaacggct gtaaagaacg gccaaggatt tggtcggttc aaatccgact tcgtcctccc
     2221 gaccggacag ccgagcctct tccatcgcct tcaaataata tccttcggcc tttttgaatt
     2281 cgggagaatt ggacggcaac caggagacgc cggggttttg attgacgagc aaaatatagt
     2341 gaagacgtcc caaattggaa tacgtctcgg cgttccgatc gtggtaaatc cgccccacc
//
LOCUS       DAOQLS010000306         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_27791_length_2395_cov_4.316667, whole genome shotgun sequence.
ACCESSION   DAOQLS010000306 DAOQLS010000000
VERSION     DAOQLS010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27791_length_2395_cov_4.316667"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="PK362_06405"
     CDS             complement(<1..1211)
                     /locus_tag="PK362_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HNC74497.1"
                     /translation="MNKFGALFVGLVAASGCLGPIRRSPSFAPAVAPAVSAPAGGAGL
                     IRLSADRTPEFLDDLDREDLGIAVRAGLRYFEKQPPERRYYFGADAYTAVEMADGLRL
                     IDGWLASDPADFGRRVREAFWVYQSTGAAPDGAIVFSAYYEHDLDASLTRTSSHTVPI
                     YGRPSDLEEAVSAGGEKTVGRRVGGALRPYWTREDIDSGGRLAGRGLEIAWAADPLDV
                     FFLQIQGSGWLRLPDGERLRIRYAGNNGHPYRSVGGTMIERGLIPRESFSRQAMKDYL
                     RTHADTRQSLLNVNPRYVFFQIDRSDLKDAAFGSLNVPLTPRRSIATDPAVYPPGALA
                     WMETDTKNKTSRFVLNQDEGGAIKGLGRVDYFVGGGPAAETYAVGFWEKGRIYFLAKR
                     RGGTERGSRWRR"
     gene            complement(1204..2004)
                     /locus_tag="PK362_06410"
     CDS             complement(1204..2004)
                     /locus_tag="PK362_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74498.1"
                     /translation="MTSRPAGWRRTFRTWVRGGALLGLAAAFLSGCVYLRLLEIKKQL
                     KNFDAHFVIGGRSELVIGFNDPVLKIKDVKYLIGAEPLYQTAEGEDVFHYEFEKTPAP
                     GPAAPVVTGTTTFVLTEATAPAAGPPPLAQLSLDLAFGEGKLVKIIVPETFMLLFPRN
                     VLVETLKQAANAEVFETKRLARGHIALPPHIEAELPSLSKTLFLLGEPAMSYPEGDLQ
                     TFVYNFRIAKDTRRVPIQGRLSFDARGLLRRVFVRWDISSVEALFQRE"
     gene            complement(2001..>2395)
                     /locus_tag="PK362_06415"
     CDS             complement(2001..>2395)
                     /locus_tag="PK362_06415"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNC74499.1"
                     /translation="WDLPPDTMAGELNLTVATTGEFLPVKFGGLSNQPALIRDFSLVF
                     PSTVTWSSVVFWIHGQSDSVESVRLFDVFTGAGVPEGQRSLAFRVSFRHADRTLSDAE
                     GAALHERVLKGLADTFHAQPRAAAPPAP"
BASE COUNT          495 a          760 c          736 g          404 t
ORIGIN      
        1 cgtcgccagc gcgagccccg ctccgttccg ccccgccgtt tggccaaaaa gtaaattcga
       61 cccttttccc aaaatccgac ggcatacgtt tcggccgcgg gtccaccgcc cacgaaataa
      121 tccacgcgac ccaacccctt gatcgcgccg ccttcgtcct ggttgaggac aaaccgggac
      181 gttttgtttt tcgtgtccgt ttccatccag gccagagccc cggggggata gaccgccggg
      241 tcggtggcga tggagcgccg cggcgttaac ggaacgttga gagacccgaa ggccgcgtct
      301 ttcagatcgg atcgatcgat ttgaaaaaaa acgtaacggg gattcacgtt caaaagcgat
      361 tgccgggtgt ccgcgtgggt ccgaagataa tctttcatcg cttgacggga gaacgattcc
      421 cgggggatca acccccgctc gatcatcgtc ccgcccaccg agcggtaagg gtgcccgttg
      481 tttccggcgt accggatacg caaccgttct ccgtccggca gtcgcaacca ccccgaccct
      541 tgaatctgaa gaaaaaaaac gtccagggga tccgccgccc aggcgatttc gaggccccgt
      601 cccgccaatc gtccgccgga atcgatgtcc tcccgggtcc aatagggccg cagggcgccg
      661 cccacccgtc ggccgaccgt cttttcgcct ccggccgaga ccgcttcctc cagatcggac
      721 ggccgaccgt aaatcgggac cgtgtgggac gaggtgcggg tcaaggaggc gtcgaggtcg
      781 tgttcgtagt aagccgaaaa aacgatggcc ccgtccggcg cggcgccggt cgattgatag
      841 acccaaaaag cctcccggac gcgccgcccg aaatccgcgg ggtccgacgc gagccaaccg
      901 tcgatcaatc gaagtccgtc ggccatttcc acggcggtgt aggcatcggc gccgaaataa
      961 taccggcgtt ccggcggttg tttttcgaaa taccgcaggc cggcgcgcac ggcgatgccc
     1021 aaatcttccc ggtccaaatc gtccaggaat tccggcgtcc ggtcggcgga aaggcgaatt
     1081 aatccggcgc cgccggcggg cgcggaaacc gccggggcga cggcgggcgc gaaggaaggg
     1141 gaacgacgga tggggcccaa acagcccgac gcggccacca ggccgacgaa gagagcgccg
     1201 aacttattca cgttggaaaa gcgcctccac ggaggaaatg tcccatcgga caaaaacgcg
     1261 ccgcagaagc ccccgggcgt caaagctcaa ccttccctgg atggggacgc ggcgcgtgtc
     1321 tttggcgatc cggaaattgt aaacgaacgt ttgcaggtcc ccctcggggt aggacatcgc
     1381 cggctcgccc aggagaaaca gggtttttga aagactcggc aattccgctt caatgtgcgg
     1441 cggcaacgcg atgtgtcccc gggccaagcg tttggtttca aacacttcgg cgttggccgc
     1501 ctgcttgagg gtttcgacca ggacgttccg cgggaacagg agcataaacg tttccgggac
     1561 gatgattttc accaacttcc cttcgccgaa cgccagatcc agggacaatt gggccagggg
     1621 gggcgggccc gccgccgggg ccgtcgcttc cgtcaagaca aacgtggtgg ttccggtcac
     1681 cacgggcgcc gccggtcccg gggcgggggt cttctcaaat tcgtaatgaa agacgtcctc
     1741 cccttccgcc gtttgataaa ggggttcggc gccgatcaaa tatttgacgt ccttgatttt
     1801 tagaacgggg tcgttaaaac cgatgaccag ttccgaacgc ccaccgatga caaaatgcgc
     1861 gtcaaaattc ttcagttgtt ttttaatttc gaggaggcgg agatagacgc aaccggacag
     1921 gaaggccgcc gccaacccca acagggcgcc cccccgaacc cacgttcgga aggtccggcg
     1981 ccacccggcg ggccgggacg tcatggggcg gggggcgccg cggcccgggg ttgggcgtga
     2041 aaggtgtcgg cgagaccctt gagaacgcgt tcgtgcaacg cggccccttc ggcgtcgctc
     2101 agcgtgcggt cggcgtgacg gaaagaaacc cggaaggcca aactccgttg gccttccggc
     2161 actcccgctc cggtgaagac gtcaaagagg cgaacgcttt ccaccgaatc gctctggccg
     2221 tgaatccaga acaccaccga cgaccaggtc accgtcgaag ggaaaaccaa cgaaaaatcg
     2281 cggatcaagg cgggctgatt ggacaatcca ccgaacttca ccggcaaaaa ttcccccgtg
     2341 gtcgccaccg ttaagttcag ttcccccgcc atcgtgtccg ggggcagatc ccagg
//
LOCUS       DAOQLS010000307         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_28469_length_2364_cov_3.852317, whole genome shotgun sequence.
ACCESSION   DAOQLS010000307 DAOQLS010000000
VERSION     DAOQLS010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28469_length_2364_cov_3.852317"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK362_06420"
     CDS             complement(<1..534)
                     /locus_tag="PK362_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNC74500.1"
                     /translation="MSPFRRALVVFLKAPVPGLVKTRLTPVLTAEEAAGFYRALARDT
                     MAAVREWADLSEGHRPWGAYQSHPRFPDLRWAGHPVPFFEQEGANLGDRLNHAFDRMF
                     REDHRQTVVIGSDAPEITVDRLTEAFQSLDRHDAVIGPARDGGYYLLGLRKSRPALWT
                     DMPWSSDGVLAETVRRLD"
     gene            complement(735..1472)
                     /locus_tag="PK362_06425"
     CDS             complement(735..1472)
                     /locus_tag="PK362_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC74501.1"
                     /translation="MPGNSVPDFPVRPAIVIPAFNEEKAIGAVLGEIPRDRTPQIIVV
                     DNGSTDGTADAARSRGASVVAEPRRGYGRAVQAGARALSSDRNVLVILDGDHSDFPED
                     LPSLLGPLERNEADLVIGSRVLGGAPRDSLTPQQRFGNWLSCRLLALLFGQSFTDLGP
                     FRALRRDLFDRLGLTDPTYGWNVEMQIKALRAGARVREVPVRYRRRIGVSKISGTVKG
                     TVLAGIKILWTIAREAGTPAPDSPEKW"
BASE COUNT          498 a          744 c          797 g          325 t
ORIGIN      
        1 gtccaaccgc cgcaccgtct ccgccaaaac accgtcggag gaccagggca tgtcggtcca
       61 cagggcgggg cgggacttgc gaaggcccag aagataataa ccgccgtcgc gggccggccc
      121 gatcacggcg tcgtggcgat ccaacgattg aaaagcctcg gtcaatcgat cgaccgttat
      181 ttcgggagcg tcggaaccga tgacgacggt ttggcggtga tcctcgcgga acatccggtc
      241 aaaggcgtgg ttcagccggt cccccaaatt ggcgccttcc tgttcaaaaa aaggcaccgg
      301 gtgtccggcc caccgaaggt ccgggaagcg ggggtgggat tggtacgccc cccagggtcg
      361 gtgtccttcg cttaaatccg cccactcccg caccgcggcc atcgtgtccc gggccagggc
      421 ccggtaaaac ccggccgctt cttcggcggt caacaccggc gtgagtcggg ttttcaccaa
      481 ccccggcacc ggggctttta aaaaaacaac gagagcccgc cgaaagggac tcacgggctc
      541 tcgcgtcccg tcgcgaacgg acggggattc aaaagaaaac ggggagatga aagcactctt
      601 tcatctcccc gtttaactag caaccacagc cgggtccgaa catggcgtcc tccctttcgc
      661 gaaaaattct cacttcctgg aacgcgccgg agcgaaaaga gttccccgat tcggcgatct
      721 tttctagggg cattttacca tttttcgggg gaatccggcg ccggggtccc ggcctcccgg
      781 gcgatggtcc agagaatttt gatcccggcc aagaccgtgc ccttcaccgt tcccgagatc
      841 ttggaaaccc cgatccgtcg acggtaacgg accggaacct cccgcacccg ggcgccggcc
      901 cgcagggctt tgatctgcat ttcaacgttc cacccgtaag tggggtccgt caaaccgagc
      961 cgatcaaaga gatcccgccg cagggcccgg aagggaccca ggtcggtgaa ggattgccca
     1021 aaaagaaggg cgaggagtcg gcaggacagc caattgccga atcgctgttg aggcgtcagg
     1081 gagtcccggg gcgccccgcc caggacccgg gacccgatga ccaagtccgc ctcgtttcgt
     1141 tccaggggcc ccagaagaga cggcaggtct tccgggaaat ccgaatgatc cccgtccagg
     1201 atcaccaaca cgttccggtc cgaggacagg gcccgggcgc cggcttggac cgcccggccg
     1261 tatccccggc ggggttcggc gacgacggag gcgccccggg accgggcggc gtcggcggtg
     1321 ccatcggtcg acccgttgtc caccacgatg atttgcgggg ttcgatcccg ggggatttcg
     1381 cccagcacgg ccccgatggc tttttcctcg ttgaaagcgg ggatcacgat ggcgggtcgg
     1441 acggggaaat cggggacgga attcccggga atccgcgcgg gtcgccgtcg gagggcgaag
     1501 aaccaaagac cgtacgtcgt cccgtattcc acgatcggca cccagggggg cagagcccac
     1561 cggccggtgc gatcgtagac ggcgaggacg agataggaaa gggccaccaa ccccgtgaag
     1621 gccagtccgg cgggatgggg gaagaagcaa aggaacggca atagccacag ggcgtaccag
     1681 gggtgcacga cgggacttaa ggccaacgcc agcgcgacgg ccccggccgc gtaggccagg
     1741 gggtctttga accgccgggc caatcccagg gccgcgacgg tccaaaggag tcccaccccc
     1801 gggcgcagcc agcgtcccgg cgccccggtc gcggcggaaa gcgccgcgta tatccccgcg
     1861 ttaaaggacc agtcccgggc gtaggtgcgg gttccggcga acagggaggc gaaggtttcc
     1921 gtcaagggcg gcgtgaggta gaaggcgtac cccgctccga tccccagggc cataaacacg
     1981 gccgtccgcc gtccttccct tcggaaaagg agccagggaa ggagcacggc cgaccccagt
     2041 tttccgagaa aagaaagggc gctgaaaacg ccggcttgga cgaacttccg acggtggaac
     2101 gccaggagtc cggcgaaaag aaaaaagagg cccagactgt ccaggtggcc cgaggaggcg
     2161 aactcggcca cggccagggg atgccaggcg tacaccaggc aacgctccgg cggaagcccc
     2221 cgggcgcgta ggaacgaggg caaaaggaaa agaagggcca aatcgaagag gagaaaggcg
     2281 acccgctggg ccgccaacgt gggcgaaagg gcgacggcga ggcgaaacac cgcctgggcg
     2341 agcggcgggt aaatcgtggg aacg
//
LOCUS       DAOQLS010000308         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_28485_length_2363_cov_4.255633, whole genome shotgun sequence.
ACCESSION   DAOQLS010000308 DAOQLS010000000
VERSION     DAOQLS010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28485_length_2363_cov_4.255633"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PK362_06430"
     CDS             complement(<1..407)
                     /locus_tag="PK362_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC74502.1"
                     /translation="MTARRPRLAVMGGGNMGGALVGGLLAARTRRAADVVVVEIDGAR
                     RRELKKKWGVGATDDPRTAVRGADAVVLAVKPQQMPDLLGALAGAVRPSQVMVSVAAG
                     VTTASIENALGPVPVVRAMPNTPALIRAGAMVY"
     gene            complement(404..1120)
                     /locus_tag="PK362_06435"
     CDS             complement(404..1120)
                     /locus_tag="PK362_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNC74503.1"
                     /translation="MSSPVDLFSLSTAHERVQERLLHAARRAGRRPEDIDLVAVTKNQ
                     PPDRAAEAVRLGYRQLGESRVQEAAGKRVALADLGATDVRWHLIGHLQSNKARRAVEL
                     FDCVQSVDSLKLAHLLNQEGERRAQPVSCLVEVKISDEPAKQGLPPGELERFLTDTAR
                     LSFLRIEGLMGVAPLGESPEEARPSFRKLHALFERFNPHFLGGRPILSMGMSQDFEVA
                     IEEGSTMIRVGTALFGPRPA"
     gene            1247..1816
                     /locus_tag="PK362_06440"
     CDS             1247..1816
                     /locus_tag="PK362_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC74504.1"
                     /translation="MRNSVFSYLRLSPWLAAAVLLTGCPKAKGPEPETPATETAAPAA
                     TETAAPAVPTLSVGTDWAGAPGFLDPVYFEGMRAELSENARAALKKNALVLKAVLKAS
                     PGVKIRVEGHCDERGTLEYNLALGQSRANALQTYYASLGIPRALLSGISYGEERPVCT
                     QSDEDCWRQNRRGETTLRAPAAVQIPLPK"
     gene            1813..>2363
                     /locus_tag="PK362_06445"
     CDS             1813..>2363
                     /locus_tag="PK362_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74505.1"
                     /translation="MIRRQARFAAVLVGVLPLGGCLATRQEIEDLRGDIIRLQTALNA
                     QQSAQGEFQTALKDNQDALQGNQADVLAKMGDLNRNLDVVSAHLEESDDRMASLANRL
                     DDLDKNITNRLDTVVKTMQGVKSLPAPSPSRLFTAAQSDYDHRRYDQALTTFQTYLDQ
                     YGDTERAPLAQYMIGEIHLAQSN"
BASE COUNT          463 a          794 c          727 g          379 t
ORIGIN      
        1 caatacacca tggcgccggc ccggatcagg gccggggtgt tgggcatggc ccgcaccacg
       61 ggcaccgggc ccagggcgtt ttcaatggac gcggtggtaa ccccggccgc gaccgagacc
      121 atcacctggg acggccggac cgcgccggcc aaggcgccca aaagatccgg catttgctgg
      181 ggcttgacgg ccaggaccac ggcgtcggct ccccgaacgg cggtccgggg gtcgtcggtg
      241 gcgcccacgc cccatttctt ctttaattcg cggcggcggg cgccgtcgat ttcaacgacg
      301 acgacgtcgg cggcccggcg ggttcgggcg gccaaaaggc cgcccacgag ggccccgccc
      361 atgttgcccc cgcccatcac ggcgaggcgc ggtcgacggg cggtcacgcg ggtcgggggc
      421 cgaaaagcgc cgtgcccacg cggatcatcg tgctgccttc ttcaatggcc acctcaaaat
      481 cctgggacat gcccatggag aggatgggcc gcccaccgag aaaatgcggg ttgaaccgct
      541 caaacaacgc gtgcagcttg cgaaaagaag gccgggcttc ttcgggggat tcgcccaggg
      601 gggccacccc catcaatcct tcaatgcgca aaaaagacag ccgcgcggtg tcggtcagga
      661 accgctccag ctctcccggg ggaagcccct gcttggccgg ttcgtccgaa attttgactt
      721 ccaccaggca ggacacgggc tgggcccggc gctcgccttc ctggttcaaa aggtgggcca
      781 atttcaggct gtccaccgat tgcacgcaat caaaaagttc cacggcccgg cgcgccttgt
      841 tcgactggag atggccgatc aaatgccacc gcacgtccgt ggcgcccaaa tccgccagag
      901 cgactcgttt gccggccgct tcttgaacac ggctctcgcc cagctgccgg tatcccaggc
      961 ggacggcctc cgccgcccgg tcggggggct ggtttttcgt cacggcgacc agatcgatgt
     1021 cctccgggcg ccggccggca cggcgggcgg catgaaggag tcgttcctga acacgctcgt
     1081 gggcggtcga cagcgaaaaa aggtccaccg gggaagacat ggtttgagac gtaaagaata
     1141 gcagattttg atccggaccg caacgcgctt gccagacggc gggggaatat gattaactaa
     1201 cctcgccccc acaattttgc cactcacgaa acaaggagcc ccaaaaatgc gaaattccgt
     1261 tttttcttac ctccgattgt ccccctggct ggccgccgcg gttcttttga ccgggtgccc
     1321 caaggccaag ggtccggagc cggaaacgcc ggcgacggaa acggccgccc ccgcggcgac
     1381 cgaaaccgcc gccccggcgg ttccgaccct gtcggtcggc acggattggg ccggcgcccc
     1441 cggtttcctg gaccccgttt attttgaagg catgcgggcc gagctttccg aaaacgcccg
     1501 ggccgccctc aagaaaaacg cgttggtttt gaaggcggtt ctcaaagcct cccccggcgt
     1561 caaaatccga gtcgaaggcc attgcgacga acggggaacc ctggaataca atttggccct
     1621 gggccaaagc cgggccaacg ccctgcagac ctattacgcg tccctgggca ttccccgggc
     1681 cctcctgagc ggcatttcct acggggagga gcgaccggtg tgcacccaat cggacgaaga
     1741 ttgctggcgt caaaaccggc ggggcgaaac gaccctgcgg gcaccggccg ccgttcagat
     1801 cccccttcca aaatgattcg gcgccaggcc cggttcgcgg cggtcttggt cggtgtcctc
     1861 cccctggggg gctgcctggc cacccgccag gagatcgagg atttgcgggg cgatattatc
     1921 cgcctgcaaa ccgccttgaa cgcccaacag agcgcccagg gagaattcca aaccgccctg
     1981 aaagataacc aggacgccct tcaagggaat caagccgacg tgctggccaa gatgggggac
     2041 ctgaatcgga atttggacgt ggtgtccgcc catttggagg aaagcgacga ccggatggct
     2101 tccctggcca atcgactgga cgatttggac aaaaacatca ccaaccggtt ggacacggtg
     2161 gtgaaaacca tgcagggggt caaatccctg cccgccccgt cgccctcccg tctcttcacg
     2221 gccgcccaaa gcgactacga ccaccgccgc tacgaccagg ccctcaccac cttccaaact
     2281 tatttggacc agtacgggga caccgaacgc gcgcccctgg cccaatacat gatcggcgaa
     2341 atccacctgg cccaaagcaa ttg
//
LOCUS       DAOQLS010000309         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_28491_length_2363_cov_3.220971, whole genome shotgun sequence.
ACCESSION   DAOQLS010000309 DAOQLS010000000
VERSION     DAOQLS010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28491_length_2363_cov_3.220971"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="PK362_06450"
     CDS             <1..1669
                     /locus_tag="PK362_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74506.1"
                     /translation="VDVPLFCNIPQFYDYDIRGRGNWAPMTTSMFRDFPILTPKLIFG
                     GAYQMRHLDFENFHDVALTTEVTRMLGAVAVNGDAEKVNPALEGRLPALPPFEPLAES
                     PLPVVCAELQTGIMRDRPRLYPQQVALNVKSSVGQGLSGLNAYMFAGGVNPPGYGAFG
                     TYHDWQAPIGPKGETRPHLEPLKEFGRFIAWAGSFLAQTKKVVDTTFGFYLPYYGTEY
                     WAGPWVDGIEGQRTNLWYDGVARLVQLAGYSYDYTDVQRSTLDVLRRHKSLWVYSLDF
                     MDHDTQAKLAAYVREGGKLILGPRLPVKDLRGEADTTLADALGLSVTGEQKGNLFKEN
                     GTDQWVQGPIQTYATEAKARTRADLPSGAGAVHTRVGRGEAVVVGFGLPHVFDHFREW
                     VGDWAKGLGLVPKVICSPWDIQAVLRCNEEFGFLFVFNYHFVERKAAVSLPRPGGKGL
                     WRLPATGTLTLAPLSGVILPLNIPLDRNVRLVYATAEVLSALARGRKVKLVFATLPGG
                     QYEVRVALPAKPRVAISGGKSAAAWSAGEARLNFEAEGAETEIQLTW"
     gene            1714..>2363
                     /locus_tag="PK362_06455"
     CDS             1714..>2363
                     /locus_tag="PK362_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HNC74507.1"
                     /translation="MPNPSFPYRTAYGQFAEDGASFIIHRPDTPRPWVNIMSSGTYGV
                     AMSQTGSGYSWLNHATLNRITRWNQDLIQDVMGRTIYMRDDKTGKFWSVGWQPVQAKP
                     ESYEVIHGVGYTIITSQNEGIQAQWLVFVPEGEPLEIWRLRIKNTSKTPRSLSLWTYL
                     EWNLGESPDWHREFHRTFIETAFQDDKKIVLAKKRMSPLKNAKGQLWNRTWDHVAW"
BASE COUNT          455 a          727 c          727 g          454 t
ORIGIN      
        1 cgtcgacgtc ccgctcttct gcaacattcc ccagttttac gactacgaca ttcggggccg
       61 gggcaattgg gcgcccatga cgacttcgat gttccgggat ttcccgattt tgaccccgaa
      121 attgattttc ggcggggcct atcaaatgcg ccatttggat tttgaaaact tccacgacgt
      181 ggccctgacc acggaagtga cccgcatgtt gggcgcggtg gcggtaaacg gcgacgcgga
      241 aaaagtgaac ccggccctcg aaggccgttt gcccgccctg ccgcccttcg agcctctggc
      301 cgagtcgccg ctgcccgtcg tctgcgccga gcttcagacc ggcatcatgc gggaccgtcc
      361 ccgactttat ccccagcagg tggcgctcaa cgtcaaaagt tccgtggggc aggggctttc
      421 cggcttaaac gcctacatgt tcgccggcgg cgtcaacccg ccgggttacg gcgccttcgg
      481 cacctaccac gattggcagg cccccatcgg ccccaagggg gagactcgcc cccacctcga
      541 acctttgaag gagttcggcc gttttatcgc atgggcgggt tcgtttctgg cccaaacgaa
      601 gaaggtcgtg gacaccacct tcggttttta tctgccctat tacggcacgg aatactgggc
      661 cgggccctgg gtggacggca ttgaagggca gcgaacaaat ctttggtacg acggcgtggc
      721 gcggctcgtt caactggccg gctacagtta cgactacacg gacgttcaac gatcgaccct
      781 ggacgttctg cggcggcaca agagcctctg ggtctactcc ctggatttta tggaccacga
      841 cacccaggcc aaattggcgg cctacgtgcg cgaaggggga aaattaattc tgggtccccg
      901 acttcccgtg aaggaccttc ggggcgaggc cgacacgacg ttggcggacg ccctgggcct
      961 ttcggtcacg ggggagcaaa agggcaattt gttcaaggag aacgggaccg accaatgggt
     1021 gcagggcccc attcaaacct acgccaccga agccaaggcg cgcacccggg cggatttgcc
     1081 gtcgggcgcg ggagccgtcc acacccgcgt cggccggggc gaagccgtgg tggtcggctt
     1141 tgggttgccc cacgtgttcg atcactttcg ggaatgggtg ggggattggg ccaagggact
     1201 gggtttggtg cccaaagtga tttgctcgcc ttgggacata caggccgtcc tgcggtgcaa
     1261 cgaagagttt ggtttccttt tcgtgttcaa ttaccatttc gtcgagcgga aggccgcggt
     1321 ctccctgccc cggcccggcg gcaaaggcct ttggcgattg cccgccaccg gaaccttgac
     1381 gttggcgccc ctctcgggag tgattttgcc tctcaatatt ccgctggacc gaaacgtccg
     1441 tcttgtgtac gctacagccg aggtcctgag cgctctggcg cggggacgca aagtgaaact
     1501 ggtgttcgcc accctgcccg ggggccaata cgaggttcgc gtcgccttgc cggccaagcc
     1561 ccgcgtggcc atctccggcg gaaagtcggc ggccgcctgg agcgcgggcg aggcccgtct
     1621 gaattttgaa gccgaaggcg ctgaaaccga gattcaactg acctggtaga gacgcctttc
     1681 gaggcgccgg aatcgttttt aaggagatcc cccatgccca acccaagctt tccttaccgc
     1741 acggcctacg gccaattcgc cgaggacggc gcgagtttca tcatccaccg gccggacacg
     1801 ccccggcctt gggtcaacat catgtcgtcg gggacctacg gcgtggccat gtcccagacg
     1861 gggagcggct acagctggtt gaaccacgcc accttgaacc gaatcacccg gtggaatcag
     1921 gatttgattc aggacgtgat gggccggacg atttacatgc gggacgacaa aaccggaaag
     1981 ttctggtccg tcggatggca gccggtccag gcgaagcccg aaagttacga agtcatccac
     2041 ggcgtgggct acacgatcat cacctcccag aacgaaggga ttcaggccca gtggctggtc
     2101 tttgtgccgg aaggcgagcc gctggaaatc tggcggttgc gcatcaagaa cacgtcaaaa
     2161 acgccccgct ccctgagcct ctggacttat ttggaatgga atttgggcga gtccccggac
     2221 tggcaccgag agttccatcg gaccttcatc gagaccgctt tccaggacga caagaaaatc
     2281 gtcctcgcca aaaaacgcat gtcgcccctc aaaaacgcca aggggcagct gtggaaccgc
     2341 acctgggacc acgtggcctg gca
//
LOCUS       DAOQLS010000310         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_28656_length_2355_cov_4.619565, whole genome shotgun sequence.
ACCESSION   DAOQLS010000310 DAOQLS010000000
VERSION     DAOQLS010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28656_length_2355_cov_4.619565"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /gene="glmU"
                     /locus_tag="PK362_06460"
     CDS             complement(<1..1331)
                     /gene="glmU"
                     /locus_tag="PK362_06460"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNC74508.1"
                     /translation="MNSINVLVLAAGEGTRMKSDLPKVLHRAAGRSLLEHVLEAAGPL
                     QGAAGVVLGRGADRVKESLAGRKNLFFFIQKERRGSGDAVRPAAGWLSRRGGDVVVLC
                     GDAPLIRPQTLKALVARHRREKNAATLLTATVVDPTGYGRIRRGLDDRPRAIVEHRDA
                     SPEDRAIREINSGTYCFRVRDLLEGLKRLRPDNAKGEYYITDVIGYFVARGRRVGAHC
                     LPDGDEILGVNNRRELAEADRLLNRRAVDALMDGGVTVLDPATTRVDATVRVGRDTVI
                     EPNTFLVGRTTIGRGCRVGPFARLEDTTVADGAQVRATFSTGARVGRGAQVGPFAHLR
                     SGTDIGPNARVGNFVEIKKSRLAAGVKAGHLTYLGDATVGAGVNIGAGVITCNYDGYA
                     KHPTVIGAGAFIGSNANLVAPLRVGAGAVVGAGSTLTRDVPTDALALERAPA"
     gene            complement(1464..1535)
                     /locus_tag="PK362_06465"
     tRNA            complement(1464..1535)
                     /locus_tag="PK362_06465"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1501..1503),aa:Gln,seq:ttg)
     gene            complement(1633..1711)
                     /locus_tag="PK362_06470"
     tRNA            complement(1633..1711)
                     /locus_tag="PK362_06470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1680..1682),aa:Gln,seq:ttg)
     gene            complement(1882..2331)
                     /locus_tag="PK362_06475"
     CDS             complement(1882..2331)
                     /locus_tag="PK362_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74509.1"
                     /translation="MNRRRMGRAALALAAVVGIAAPSWAEDLVFVVSSEVSIEINKDL
                     VKDVYWGRRLYWPKIGKQVKLCLRAAQSPLNDVLLRDFLMTSQQRYDRYWKSQMLAGR
                     SSGMENLKDLDAVIDFTSRNPGSIAFMSVQEAERVVNPRIRKIRIAP"
BASE COUNT          436 a          775 c          739 g          405 t
ORIGIN      
        1 gcgggggcgc gctccagggc cagcgcgtcc gtcgggacgt cccgggtgag cgtcgatccc
       61 gcccccacca cggcccccgc gccgacccgg aggggagcca ccaggttcgc gttggagccg
      121 atgaacgccc cggcgccgat gaccgtcggg tgcttggcgt aaccgtcgta gttgcaggtg
      181 atgacgccgg cgccgatgtt cacgcccgcg cccaccgtcg cgtcgcccag atacgtcaga
      241 tggccggcct tgacgcccgc cgccagccgg gatttcttga tttccacaaa attgccgacc
      301 cgggcgttgg ggccgatgtc cgtgcccgat cgaagatgag cgaaggggcc cacctgggcg
      361 ccccgcccga cccgggcgcc ggtcgaaaac gtcgcgcgca cctgggcgcc gtcggccacg
      421 gtcgtgtctt ccagccgggc gaaggggccc acccggcaac cgcggccgat ggtcgtgcgc
      481 cccaccaaaa acgtattcgg ttcaatcacc gtgtcgcggc ccacgcggac ggtggcgtcc
      541 acccgggtgg tcgccgggtc caacaccgtg accccgccgt ccatcaaggc gtccaccgcc
      601 cggcggttca acagccggtc ggcttccgcc agttcccggc ggttgttgac ccccaaaatc
      661 tcgtcgccgt cgggcaggca atgggccccc acgcggcggc cccgggccac gaaatacccg
      721 atcacgtcgg tgatgtagta ttcgcccttg gcgttgtcgg ggcggaggcg cttcaagccc
      781 tccaacaaat cccgtacgcg aaaacagtag gtgccggaat tgatttcccg aatggcgcgg
      841 tcttcggggc tcgcgtcccg gtgctccacg atggcccggg gacggtcgtc cagcccgcgg
      901 cggatgcgcc cgtacccggt gggatcgacc acggtggcgg tcaggagcgt ggcggcgttt
      961 ttttcccgtc ggtggcgcgc caccagggct ttcaaagtct ggggccggat gagcggcgcg
     1021 tcgccgcaga ggaccaccac gtcccccccc cggcggctca gccaaccggc ggcgggacga
     1081 acggcgtcgc cggacccccg gcgttccttt tgaatgaaaa agaacaaatt tttccggccc
     1141 gccaggcttt ccttcacccg gtccgcgccg cggcccaaca ccacgccagc cgccccctgg
     1201 agcggtccgg cggcttccaa gacgtgttcc aaaaggcttc gaccggccgc ccggtgaagg
     1261 actttgggca aatcggattt catccgggtg ccttccccgg ccgcgagaac aagaacgttg
     1321 atggaattca agagagaagt tttagcctaa aagaacggcg ttattttagc aaaatttcgc
     1381 ccggatgggg agccagggat cgctcttggt aaggtccccc gccggcacgc tcggacaacg
     1441 ctgctcaatt ttaaagaaca ttactggggc gctaggattc gaacctagga atggcggctc
     1501 caaaggccgc tgccttacca cttggctacg ccccaggaat gttctctgaa aaatggaaac
     1561 gcttcgttga gcggatatcc gctacaaatt gccgagcgac aaagtcgctc tccccaaccg
     1621 cgaacctggg aatggcggcg ccgctaggtg ccgtgccgga ttgtcggacg gcgcggctcc
     1681 aaaggccgct gccttaccac ttggctacgc cccaggaatg ttctctcgaa gacggtgaaa
     1741 ataaatttgg cgcccgcggc ccagaggccg acggggacac atcatattaa attacgctgg
     1801 aaaggtccgg tgggacaata aaaaacgggt gggagcggag cccccacccg gtcgaaagcc
     1861 gtcccgacgg ggacgacgtt tttacggcgc gatgcggatt ttccgaatgc gcggattgac
     1921 gacgcgttcg gcctcttgaa ccgacataaa agcgatggaa ccggggttgc gggaggtgaa
     1981 gtcgatcacc gcgtccaggt ccttcaaatt ttccatcccg gagcttcgtc cggccagcat
     2041 ttggcttttc cagtaacgat cgtaacgttg ctgggaggtc atgaggaaat cgcgcaacag
     2101 gacgtcgttg agcggcgact gggccgcgcg caggcacaat ttcacctgct ttccgatctt
     2161 gggccaatag aggcgacggc cccaatacac gtctttcacc aggtctttgt tgatttcgat
     2221 ggacacttcg gaactgacga caaaaaccag gtcctcggcc caggagggcg ccgcgattcc
     2281 aacgacggcc gcgagcgcca aggcggcgcg tcccattcgc ctgcgattca tggtgtcgat
     2341 cctccccgcc tacac
//
LOCUS       DAOQLS010000311         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_28753_length_2351_cov_3.881098, whole genome shotgun sequence.
ACCESSION   DAOQLS010000311 DAOQLS010000000
VERSION     DAOQLS010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28753_length_2351_cov_3.881098"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /gene="purD"
                     /locus_tag="PK362_06480"
     CDS             <1..1072
                     /gene="purD"
                     /locus_tag="PK362_06480"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNC74510.1"
                     /translation="RGGLTVFGPGAAAARLEGSKLFAKNFMKAHGLPTADFRSFTRAA
                     DAEAFVKGEGWNTGFRVVKADGLAAGKGVVVGKNHGDVLAALTDMMVRKRFGDAAQTV
                     LIEDTLVGEELSVMALTDGKTLVPLLATQDHKRVFDGDQGPNTGGMGAYGPVPQVTAD
                     TWRRIETEVFDRFLAGLAADRLDYRGVIYFGLMLTADGPKVLEFNVRFGDPETQVILP
                     MGKGDWLPLFLNTAQGTLAGQSFVPRSGAAITVVMASGGYPGDFAKGKVINGLDLLEN
                     DKDVAVFHAGTAHDAQGRYVTAGGRVLAVTARGADLTGAREKAYAAVRRVAFEGVHYR
                     RDIGARALPPAGETSRPHASKK"
     gene            1089..1640
                     /gene="pyrR"
                     /locus_tag="PK362_06485"
     CDS             1089..1640
                     /gene="pyrR"
                     /locus_tag="PK362_06485"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNC74511.1"
                     /translation="MTAKRLFNEAEVRAAIGDIARGIYQRVPPPRSGWAIVGIQRRGV
                     PLAQRLARALEELGAPALPVGSLDITFYRDDMGETALHPVVHETELSFDLSGKILFLV
                     DDVLYTGRTIRSALNELMDFGRPSRIYLVALVDRGHRELPIQADFVGKTVPTQKEERV
                     DIHLTEIDGDDGVWLSPRAVKTA"
     gene            1637..>2351
                     /locus_tag="PK362_06490"
     CDS             1637..>2351
                     /locus_tag="PK362_06490"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNC74512.1"
                     /translation="MKDLLGLEALTPAQIEHILDVARPMKSLFTRSIKKVPALRGKTV
                     ALLFFEASTRTRSSFELAAKRLSADTLSFAASTSSVTKGESLLDTAKTYEAMKVDFMV
                     IRHAASGAPELVAQAVKARVINAGDGSHEHPTQGLLDIYTILEKKKRLDGLKVVIVGD
                     ILHSRVARSNLWGLTKLGAHVTVCGPATLIPAGLPEVFGPKVTVATRLDEALEGADVI
                     NVLRLQRERQQGDYFPTVRE"
BASE COUNT          413 a          797 c          758 g          383 t
ORIGIN      
        1 gcggggcggc ctgacggtct tcgggcccgg ggcggccgcg gcgcggctcg agggcagcaa
       61 attgtttgcc aaaaatttca tgaaggccca cgggttgccc accgcggatt ttcgttcctt
      121 cacccgggcc gcggacgccg aggcgttcgt gaaaggcgag ggatggaaca ccggattccg
      181 tgtggtcaaa gccgacggcc tcgcggccgg aaaaggcgtc gtggtcggca aaaaccacgg
      241 ggacgtcctg gcggcgctca cggacatgat ggtccgaaaa cgcttcgggg acgcggccca
      301 gacggtgttg atcgaggaca ccttggtcgg ggaggaactc agcgtcatgg ccctgaccga
      361 cggaaaaacc ctggtgcccc tcctggcgac ccaggaccac aagcgcgttt ttgacggcga
      421 ccaggggccc aacaccggcg gtatgggcgc gtacggcccg gtcccccaag tgaccgccga
      481 cacgtggcgg cgcatcgaga cggaggtgtt cgaccgtttc ctggccggat tggccgccga
      541 ccggctggac taccggggcg tcatttattt cggcctcatg ctcaccgccg acggccccaa
      601 agttctcgaa ttcaacgttc gcttcgggga cccggaaacc caggtgattc tgcccatggg
      661 gaagggggac tggctgccgc tcttcttgaa caccgcccag ggcacgctgg cgggccaatc
      721 cttcgtgccc cggagcggcg cggccatcac ggtcgtgatg gcctccgggg ggtatccggg
      781 ggatttcgcc aagggcaagg tgatcaatgg cctggacctt ctggagaacg acaaggacgt
      841 cgccgtgttt cacgccggca ccgcccacga cgcccagggc cggtacgtga cggcgggggg
      901 gcgcgtcctg gccgtgacgg cccggggcgc ggatttgacc ggggcgcggg aaaaagccta
      961 cgccgccgtc cgccgcgtcg ctttcgaagg cgtgcattac cgccgggaca tcggcgcccg
     1021 ggccctgccg ccggccgggg aaacgtcccg tccgcacgcc tcgaaaaaat gaaataataa
     1081 gccttggcat gaccgccaag cgtctcttca acgaagccga ggtccgcgcc gccatcgggg
     1141 atatcgcccg gggcatttac cagcgcgttc ccccgccgcg cagcggctgg gcgatcgtcg
     1201 gcattcagcg ccgcggggtc cccctggccc aacggctcgc ccgggcgttg gaggaactcg
     1261 gcgccccggc cctgcccgtg ggttccctcg acatcacctt ctaccgcgac gacatgggcg
     1321 aaaccgccct ccaccccgtg gtccacgaaa ccgaattgtc cttcgatttg tccggcaaga
     1381 tcctcttttt ggtcgacgac gttctctaca ccggccggac catccggtcg gcattgaacg
     1441 agttgatgga tttcggccgc ccctcccgca tttacctggt cgcccttgtg gaccggggcc
     1501 accgcgagct tcccatccag gccgatttcg tcggcaaaac ggtgccgacc caaaaggaag
     1561 agcgggtgga catccacctg acggaaatcg acggcgacga cggggtgtgg ctgtcgcccc
     1621 gggcggtgaa aaccgcgtga aggacctcct cggcctcgaa gccctgaccc ccgcccaaat
     1681 cgagcacatt ctcgacgtgg cgcggcccat gaaaagcctg ttcacccggt ccatcaagaa
     1741 agtccccgcg ctccggggca aaacggtcgc cctcctgttt ttcgaagcct ccacccgcac
     1801 gcgcagttcc ttcgagctcg cggccaaacg tctgtccgcc gacacgttga gtttcgccgc
     1861 gtccaccagt tcggtgacga agggcgaatc cctcttggac acggccaaga cctacgaggc
     1921 catgaaagtg gatttcatgg tcatccgcca cgcggcctcc ggcgcgcccg agttggtggc
     1981 ccaggcggtc aaggcccggg tcatcaacgc gggggacggt tcccacgaac accccaccca
     2041 gggccttctg gacatttaca cgatcctgga aaaaaagaaa cgcctcgacg gcctcaaggt
     2101 cgtcatcgtg ggcgacatcc tccattcccg ggtggcccga tccaacctct gggggttgac
     2161 caaactgggc gcccacgtca ccgtgtgcgg ccccgcgacc ctcatcccgg cgggactccc
     2221 cgaagtgttc ggccccaagg tgaccgtggc cactcgtttg gacgaggccc tggagggcgc
     2281 ggacgtcatc aacgtgctgc ggctccagcg ggagcggcaa cagggcgatt attttccgac
     2341 ggtgcgcgaa t
//
LOCUS       DAOQLS010000312         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_29107_length_2337_cov_4.235320, whole genome shotgun sequence.
ACCESSION   DAOQLS010000312 DAOQLS010000000
VERSION     DAOQLS010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29107_length_2337_cov_4.235320"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2337
                     /locus_tag="PK362_06495"
     CDS             <1..>2337
                     /locus_tag="PK362_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74513.1"
                     /translation="PMSLAGGVLGMAAAGFNLGVLEILLIGLQAGFLWSLAPAQKKFV
                     DDHGAQQSERQAALRRRGTILLNVAAVLGGSLAPLARAYFGVDASLIYFLVASPATGL
                     LAAMTVHVLWNAAAPARYQLTRAPPESPSAPTPLGLLDRLPLLATTFATRLALYAEGE
                     RISEVEALRRVRRLSQYPFRDPLQNTANQMTNYYYMLIALSGRTNGHGRRGEAEGRQA
                     DLPLLRLVLLDRAVSLARAALPLVVKSTGKTDMSEALRDQIKWDVRLFAPLAASLPRD
                     RSSGIAGMDLAAVMTDLVVFLENPARYEKTRRELLGLVGDSSRLHDVQQALLSEADSL
                     RPIGAPRWMTQARIKTVSSVVNKVIVNPNYSAAAEVKDIVTLSIVFDLVGARQSVATW
                     QEWRTMVYQLRDRLEERLRDKGRNVTLTLPDEKEQGYIHFNANVDGVPCEMQFQTMAI
                     HQRQEAGDRIARVGGQAHYVRKANQLMAWLGKQIGFAPIKLSPHRRLFEGDTEADFEM
                     LFAKARKYTVVTLLSREQIKNPEAPADTVLLPAGATPMDLLASTAVADYEFTFQEFPD
                     IVDCPWDGRAFKDGNDSSKLGMNNPMATGALVRLGAKREPIVGAGERNAIRSAGRTFR
                     LWAKGIIDSKDRERFTAAGQAFLADLGIETSFMARRVFRADSTLEAFLRKYDLTPDML
                     ALLVGEEISSRTNMEYRPVDRLIIPRLINAYSRTGETMLGQEGLDLGHPRVQEELRSF
                     ALSRMTTENKFNALLPRVLASSGRTVEIMALAAGVGLG"
BASE COUNT          432 a          691 c          732 g          482 t
ORIGIN      
        1 gcccatgagc ctggcggggg gcgttctcgg gatggccgcc gccggcttca acttgggagt
       61 cctggagatc cttctcatcg gccttcaggc cggattcctc tggagtctgg ctccggcgca
      121 aaaaaaattc gtcgacgatc acggcgccca acaatccgag cgccaagcgg ctcttcggcg
      181 gcggggcacc atccttttaa acgtcgcggc ggttttgggc gggtctctgg cgcctctcgc
      241 tcgggcgtat ttcggcgtgg acgcttccct gatttatttc ctcgtcgctt caccggcgac
      301 ggggcttttg gcggccatga cggtccacgt tttatggaac gccgctgctc ccgcccggta
      361 tcagctcacg cgcgcgccgc cggaatcgcc gtccgctccc acgcctctcg ggttgttgga
      421 tcgtttgcca ctgttggcca cgacgtttgc cacccggttg gcgctctacg ccgaagggga
      481 gcggatctcc gaggtggaag ctttgcgacg ggttcgccgt ctatcgcaat acccttttcg
      541 agaccccctc caaaacaccg ccaaccaaat gacgaattat tactacatgc tcatcgcgtt
      601 gagcggccga accaacggcc atggccgtcg cggggaggcg gaagggcgcc aggccgatct
      661 gccgttgttg cggcttgtcc ttttggatcg ggcggtgtcg ttggcgcgcg cggccttgcc
      721 gttggtcgtt aaatcgacgg gaaaaaccga catgtcggaa gcccttcggg accagattaa
      781 atgggacgtt cggttgttcg ccccgttggc cgcgagcttg ccgcgggacc gctcgtcggg
      841 gatcgccggc atggacctcg ccgcggtcat gaccgatttg gtggtcttcc ttgaaaaccc
      901 cgcgcgatac gaaaaaaccc gccgggaact tttggggctg gtcggcgatt cgtcccgcct
      961 gcacgacgtc cagcaagcgc tgctgagcga ggcggactcc ttgcgtccca tcggcgcccc
     1021 ccgatggatg actcaggccc ggataaaaac ggtttcctcg gtggtcaaca aggtgatcgt
     1081 caacccgaat tacagcgcgg ccgcggaagt caaggacatc gtgaccttgt cgatcgtttt
     1141 cgatttggtt ggggcgcgac aatcggtcgc cacgtggcag gaatggcgca cgatggtcta
     1201 ccaattgagg gaccgcttgg aagagcgatt gcgcgacaaa ggaaggaatg tgacgttgac
     1261 cctgccggac gaaaaagagc aagggtatat ccatttcaac gccaacgtcg atggcgtccc
     1321 ctgcgaaatg caatttcaaa ccatggccat ccatcagcgc caggaggcgg gcgatcggat
     1381 cgcccgggtg ggaggtcagg cgcattacgt acgcaaggcc aaccaattaa tggcctggtt
     1441 gggcaagcag atcggattcg cgccgatcaa actcagcccg caccggcggc ttttcgaagg
     1501 ggacaccgag gcggattttg aaatgctgtt tgcgaaggcg cgcaagtata cggtcgtcac
     1561 cctcctctcc cgcgaacaaa tcaagaaccc cgaggcgccc gccgacacgg tgctattgcc
     1621 cgccggggcg acgcccatgg atttgctggc gagcacggcg gtggccgatt acgagttcac
     1681 gttccaggaa tttcccgata tcgtggattg cccctgggac ggacgcgcat tcaaggacgg
     1741 caacgattca tcgaaattgg gcatgaacaa tccgatggcg accggcgcgc tcgtccgttt
     1801 gggcgcgaag cgcgagccca tcgtgggggc cggggagcgg aacgccatac gaagcgccgg
     1861 tcgtactttt cgcctgtggg ccaaaggcat tatcgattcc aaagaccgag agcggtttac
     1921 ggccgcgggg caggcgtttt tggccgattt ggggattgaa acttcgttta tggcacggcg
     1981 agtttttcgg gccgattcga ccttggaggc ctttctacga aaatacgacc tcacccccga
     2041 tatgttggca ttgttggtgg gggaggaaat atcctcccga acgaacatgg agtaccgccc
     2101 cgtggatcgg ttgatcatcc cgcgccttat caacgcttac agtcgcacgg gggaaaccat
     2161 gctcggccag gaagggctgg atttgggcca tccccgtgtc caggaagagc ttcgctcttt
     2221 cgctctctcc cgcatgacca ccgagaataa attcaatgct cttttgcctc gcgttctcgc
     2281 ctcgtcgggt cgaacggtcg agatcatggc gttggccgcg ggcgtcggtt tgggttg
//
LOCUS       DAOQLS010000313         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_29453_length_2322_cov_2.580944, whole genome shotgun sequence.
ACCESSION   DAOQLS010000313 DAOQLS010000000
VERSION     DAOQLS010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29453_length_2322_cov_2.580944"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..737)
                     /locus_tag="PK362_06500"
     CDS             complement(135..737)
                     /locus_tag="PK362_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC74514.1"
                     /translation="MNIRSLLSVLLLSVGTAAFAAPEIGKPAPAFALKDTQGKDVSLA
                     DYKGKVVVLEWINHGCPFVKKHYGSGNMQNLQKEYTAKGVVWLSIASSAPGKEGYMKA
                     DGWNKAIAEKKSAATAVLLDPDGVAGRAYGAKTTPHMFVIDAEGRLVYKGAIDDRPSA
                     DPADVPGAKNYVRQALDSVLAGKSVATPSTPPYGCGVKYK"
     gene            complement(734..>2322)
                     /locus_tag="PK362_06505"
     CDS             complement(734..>2322)
                     /locus_tag="PK362_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC74515.1"
                     /translation="RADLELRLNVGPRARPNPATAELFARFRSRLPRPPDNWTLEARR
                     SGKSVKVSLQPPPGANISSKALTLFPEDEDTAEAGAVPAGVLRDGKITVEFPSEANRV
                     QAVLVAADGPESFTAYALDLPVTQGPATAPLGWILLLAFAGGLLLNLMPCVLPVLSLK
                     AFGFLKDAGLDAASRRRQGLLYVAGVVVSFWALAGVLLALRAGGQGLGWGFHLQSPRF
                     VLVLIGLFVLLAANLWGFFEVGGRWVGAAAERGRSWGAFGSGILATVVATPCTAPFMG
                     TAIGVALAQPVPAALAVFTSLALGMAFPYWLLTVVPAAGRWLPRPGPWMNRVKMVLGF
                     FLLATAGWLLWVLYQQSPAAVGPAAALGAALGVAAWAYGQKQRGLRFRWTATTLAAVL
                     AMGSWAWMAAGSGAVSSSESGVWEVYTPERVAELEKEGRPYFIDFTAAWCLTCQVNKK
                     TTLEKPETLALFRENNVALLRADWTNRDETIARALAGWGRSGVPLYVLKGRGAPALLP
                     ALLTPGLVRDALSKMKEEAS"
BASE COUNT          432 a          785 c          743 g          362 t
ORIGIN      
        1 ggggcacccc ggcggggaga gagcacttct ttcacggctc cccccaccct caccctcccc
       61 cgcaaggggg gaggggacaa aagccgcttg tctcagcgat cctaaatcgt gagggaaatt
      121 tcgtgcgcac cccgctattt gtatttcacg ccgcagccgt agggcggggt gctcggggtg
      181 gcgaccgatt ttccggcgag cacgctgtcc aaggcctgcc ggacatagtt cttcgcgccg
      241 ggaacgtccg ccgggtccgc ggaggggcgg tcatcgatgg cgcccttgta aacaaggcgg
      301 ccttcggcgt cgatcacgaa catgtggggc gtcgtcttgg cgccgtaggc ccggcccgcc
      361 acgccgtccg ggtccaacag cacggccgtg gcggccgatt tcttttcggc gatggccttg
      421 ttccacccgt cggccttcat atagccttcc ttgcccgggg cggaggaggc gatggaaagc
      481 cacaccacgc ccttggccgt gtattccttt tgaaggttct gcatgttgcc cgaaccgtag
      541 tgctttttca cgaaggggca cccgtggttg atccactcca gaaccacgac ttttcccttg
      601 tagtccgcca gggaaacgtc cttcccctgg gtgtctttta aagcgaacgc cggcgcgggt
      661 tttccgattt ccggggccgc aaacgcggcc gttcccaccg acagcaacaa caccgacaac
      721 aacgaacgga tgttcatgaa gcctcctctt tcatcttcga taaagcgtcg cgcacgagcc
      781 cgggcgtgag cagggcgggc aggagcgccg gggcgccccg gcctttcaaa acataaagcg
      841 gcacgccgct tcggccccac ccggccaggg cccgggcgat ggtctcgtcc cggttggtcc
      901 agtcggcccg caggagcgcc acgttgtttt cgcggaacag ggccagagtc tcgggtttct
      961 ccagggtggt ttttttgttc acctggcagg tcaggcacca ggcggcggtg aaatcgatga
     1021 agtagggccg cccctctttt tccagttcgg ccacgcgctc gggggtgtaa acttcccaga
     1081 caccgctttc cgacgatgaa accgcgccgc ttcccgcggc catccaggcc caggacccca
     1141 tggccagaac cgccgccagg gtggtggccg tccaacggaa acgaaggccc cgttgctttt
     1201 gaccgtaggc ccaggcggcc acgcccaggg ccgcgcccag ggccgccgcc ggtcccaccg
     1261 ccgccgggct ctgttgatag agcacccaga ggagccaccc cgccgtcgcc agtaggaaaa
     1321 atcccaaaac cattttcacc cggttcatcc agggcccggg gcgcggcaac cagcgccccg
     1381 cggccggaac gaccgtgaga agccagtagg gaaacgccat gccgagcgcg agactggtga
     1441 acaccgccag cgccgcgggc accggttggg ccagggccac gccgatggcc gttcccataa
     1501 agggcgccgt gcagggcgtg gcgacgacgg tggccaggat gccgcttccg aaagcgcccc
     1561 aggaccgtcc ccgttccgcc gccgcgccga cccagcgccc gccgacttca aaaaatcccc
     1621 agaggttggc ggccagcagg acgaagaggc cgatcaaaac caaaacgaat cggggcgatt
     1681 gcagatggaa cccccacccc agtccctgcc cccccgcccg aagggccagc aacacccccg
     1741 ccagggccca aaaggaaacc accacccccg ccacgtagag caggccctgc cgccgacggg
     1801 aagcggcgtc caaccccgcg tctttaagga agccgaaggc cttgagcgac aggacgggca
     1861 aaacgcaggg catcaggttg agcaacaacc cgcccgcaaa ggcgagcagt aaaatccaac
     1921 cgaggggagc cgtcgcgggg ccctgggtaa caggcaggtc cagggcgtag gcggtaaatg
     1981 actcgggacc gtccgccgca accaagacgg cctgcacacg attggcttcc gatggaaatt
     2041 ccacggtgat tttcccgtcc cgaaggaccc ccgccggaac ggcgccggct tccgccgtgt
     2101 cttcatcttc ggggaaaagg gtgagggcct tggacgaaat gtttgccccc ggcggcggtt
     2161 gaagggaaac ttttacggac ttgcccgacc gtcgggcttc cagggtccag ttgtctggtg
     2221 gtcggggaag gcggcttcgg aaacgggcga aaagttcggc cgtggcgggg ttgggccggg
     2281 ccctggggcc gacattcagg cgaagctcaa ggtccgcccg gc
//
LOCUS       DAOQLS010000314         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_29467_length_2321_cov_3.715357, whole genome shotgun sequence.
ACCESSION   DAOQLS010000314 DAOQLS010000000
VERSION     DAOQLS010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29467_length_2321_cov_3.715357"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..421
                     /locus_tag="PK362_06510"
     CDS             188..421
                     /locus_tag="PK362_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNC74516.1"
                     /translation="MVTGLVLVRLNAGREKQTLKQIKETKGVAHTSAVYGRWDLVVDV
                     EAEDLPSMTHVVVEKIRAIPGVASTETLVTTAI"
     gene            421..1734
                     /locus_tag="PK362_06515"
     CDS             421..1734
                     /locus_tag="PK362_06515"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNC74517.1"
                     /translation="MTFARAAALGLVGTLMTAVTAQAGPVKRLSNGLRYVVLEDHASP
                     VVSLQIWVRCGGVNEEGPTSGVSHFLEHMIFKGTDKISANEISKIVESNGGSINAATG
                     GETTQYYIDIPSKQWEKAFDVLAESVMRPAFPPLEFEKERKVILEEIKRRNDDPRSDL
                     WDVFLEGLYRRTPYRQQVIGSVETITAMTRDLMVRQHALFYVPNNVVVVVAGDVQRAA
                     VEKKIKKIFGALPAGPTPPQPALLEPPVAEPTVQQIYNDKAQQAHVALGFVGPTLKDP
                     DQVVMDVLSTALGGGQSSRLYQKLREEKRQVWSISASFITHAGSGAFGIFAECPPEKT
                     NSLPNDVYFLLGDVEFNGFTVDEVERAKAQIRSSWLFGQETYHGQASQWGFYTALGAP
                     EVLETYLAKLDRVTAADLGRALTKYSLGRPLSGVVVKPRPAGGGR"
     gene            1741..>2321
                     /locus_tag="PK362_06520"
     CDS             1741..>2321
                     /locus_tag="PK362_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNC74518.1"
                     /translation="MKRILLLLCLMSAPAAAVEAPRVRRFDNGLTWIHRTVTHNRIVA
                     AELFLPGGDAVEPADRAGVTRLLSAVWVKGTPSRNALQIAQQMESLGAAFGIDAQEDF
                     VAIGGQVIVDNWPQTRALFEDIVFHPTLPVEEIEKEREALLNAIRTRHEHIFNVAEER
                     FRRELFGAHPYGRPDEGTEATVRALTREDLVKW"
BASE COUNT          442 a          777 c          725 g          377 t
ORIGIN      
        1 ggggcgcgcc gagggagacg cgatgcgcgc gacgcggacc ttggccctgg gacgaatcaa
       61 aatcggttta acccgggcgg ccgcgcggcc ctgggtgggt cgcctgctct gggaccccat
      121 cggatttccg cccgaccggt ggctcacgcc ccggcggcga atttaaagga cttcaagggg
      181 aggaaccatg gtgacgggat tggtgctggt tcgtttgaac gcgggacgcg agaagcagac
      241 gctcaagcag atcaaggaaa ccaaaggggt cgcccacacc tccgccgtgt acggccggtg
      301 ggacctggtc gtcgacgtcg aagccgaaga tttgccctcc atgacccacg tggtcgtcga
      361 aaagatccgg gccattccgg gagtcgcttc cacggaaacc ctggtcacga cggccatttg
      421 atgacgttcg cccgcgccgc cgccctggga cttgtgggaa cgctgatgac cgccgtgacc
      481 gcccaggccg gacccgtgaa acgcctgtcc aacggcctcc gctacgtggt gctggaggac
      541 cacgcctccc ccgtcgtttc cctgcagatc tgggtgcggt gcgggggggt gaacgaggaa
      601 ggtcccacct cgggcgtttc ccatttcttg gaacacatga tcttcaaggg gaccgacaag
      661 atttcggcca acgaaatctc gaagatcgtc gaatccaacg gcggatccat caacgccgcc
      721 accggcggcg aaaccaccca atactacatc gacattccct ccaaacaatg ggaaaaagcc
      781 tttgacgtgc tggccgaatc cgtgatgcgg ccggcgtttc cgccgttgga gtttgagaag
      841 gaacgcaaag tcattctgga ggagatcaag cgccggaacg acgacccgcg ctcggacctg
      901 tgggacgttt tcctggaggg gctttaccgg cgcacgccct accggcaaca ggtgatcggc
      961 agcgtcgaaa cgatcacggc catgacgcgg gacttgatgg tccgccagca cgccctcttc
     1021 tacgtgccca acaacgtggt ggtcgtggtg gcgggggacg ttcaacgcgc cgccgtggaa
     1081 aagaagatca aaaagatttt cggcgccctc ccggccggac cgaccccgcc ccagccggcg
     1141 cttctggaac cgccggtggc ggaacccacg gttcaacaaa tttacaacga caaagctcag
     1201 caggcccacg tggccctggg cttcgtggga cccacgttga aggacccgga ccaggtggtc
     1261 atggacgtcc tctcgaccgc cctgggcggc ggccagagtt cccgcctgta ccagaaactg
     1321 cgggaggaaa aacgccaggt ctggtccatc agcgccagct tcatcaccca cgcgggatcg
     1381 ggcgctttcg gcattttcgc ggagtgcccg cccgaaaaaa ccaactccct gcccaacgac
     1441 gtgtatttcc tcctgggcga cgtggagttc aacggtttca ccgtcgacga agtggagcgc
     1501 gccaaagccc agatccgaag ttcgtggttg ttcggccagg aaacctacca cggccaggct
     1561 tcccagtggg ggttctacac ggccctgggc gcccccgagg tgctggaaac ctatttggcc
     1621 aaactggacc gggtgaccgc ggccgacctg ggccgcgccc tcaccaaata ttccctgggc
     1681 cggcccctct ccggggtcgt cgtcaaaccc cgtcccgccg gaggaggccg ctgaactccc
     1741 gtgaaacgaa ttctcctgtt gctctgtctt atgtccgccc ccgccgccgc cgttgaggcc
     1801 ccccgggtcc gccggttcga caacggcctg acctggatcc accggaccgt cacccacaac
     1861 cgcatcgtcg cggccgagct gttcctgccg ggcggcgacg ccgtggagcc cgccgaccgg
     1921 gccggggtga cgcgcctcct ctcggccgtg tgggtcaaag gcacgccctc gcgcaacgcc
     1981 ctccaaatcg cccaacagat ggaatccctc ggcgcggcct tcggcatcga cgcccaggag
     2041 gatttcgtgg ccatcggcgg ccaggtgatc gtcgacaact ggccccaaac ccgcgccctc
     2101 ttcgaagaca tcgtgttcca ccccacgctg ccggtcgagg agatcgaaaa ggagcgggag
     2161 gcgctcctga acgccattcg aacgcggcac gaacatattt tcaacgtggc cgaggagcgg
     2221 ttccgccggg aactcttcgg cgcgcacccc tacggtcggc ccgacgaagg caccgaagcg
     2281 acggtccggg ccctcacgcg ggaggatttg gtgaaatggc a
//
LOCUS       DAOQLS010000315         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_29591_length_2315_cov_4.131416, whole genome shotgun sequence.
ACCESSION   DAOQLS010000315 DAOQLS010000000
VERSION     DAOQLS010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29591_length_2315_cov_4.131416"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..>2315
                     /locus_tag="PK362_06525"
     CDS             8..>2315
                     /locus_tag="PK362_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74519.1"
                     /translation="MKGFVRAYLKANGYRVREEGWLSGLAPHYCAPKEPNRPYADLRR
                     ELSPDNLRRLDALEPIGEGHPFNALARGLAGVLWGTWWVPVIAAGGLDGATVSWMGPV
                     LWAAISYSLLVAASNVLDGALAGLARALAPRTGEPDDDLRQKAKARLGLLDDNHLRIV
                     PQAQMNRLGKVGRWKMFSRSYYGLTIIDEKGRPEATYLADWLFRPARGRLLERLRDRV
                     LRNVLDREKERRSPSALRRHAIAIDLRRPFRFLTAPVAGSRRRTARAAAAAMAFLAGG
                     ATAQPAIDPDLNLAPLAASARSVVDAGLVKSPEGKTAVERFERFLAAGGAGTVRVGNF
                     ASLPVRFLEELDRLNDGLAKDQLMVLTRGSAGNTTVTPLRRVGPSSLSDQVLRVTNIP
                     AETQARGVGGEGGYTVRRLSLIYADRLELKAQNVRDVLQWPKGRRNLALPGWDVLIDR
                     QFPMGSAIVSLDAVRSSFEARWVIHEWLHRVGGKERDALLGEFIIAPWLQVTTLEVGL
                     IAYLGGPGAEAEPARVAAREVFRLLEKELVPGREPLLTPASTLQTLTSRGDLDLRRAA
                     IRAWVKASGRPFFTSDTLALARKAGLSSDVLASLQAEEKSALPEGTARRAQYENALAK
                     EHLGWLTAQGDSPTARRYLRAVAANAPLGPDAPLTADTVVQLLRRIRAREVDSTFNNA
                     LAPLAAERKKLPASSPAAAAHDRGTALATTLLDNLLAQVRRDHQNQVLPLSALSAAGA
                     AALSLILAATGVGTLDGPAAFLALLPGAW"
BASE COUNT          345 a          804 c          773 g          393 t
ORIGIN      
        1 cgaacgcatg aaagggttcg tccgggccta tctgaaagcg aacggatacc gggtgcggga
       61 ggaggggtgg ctgagcggtc tcgcgccaca ttactgcgct cccaaggaac cgaatcgccc
      121 ctacgccgat ctgcggaggg agctctcccc ggacaacctc cggcggcttg acgctctcga
      181 gccgatcggt gaagggcatc cttttaatgc cctggcccgc ggcctggccg gcgtcctgtg
      241 gggaacgtgg tgggtcccgg tgatcgccgc gggcgggttg gacggcgcga ccgtgtcctg
      301 gatggggccc gtcctttggg ccgcgatctc ctacagtctc ctggtcgccg cctcgaacgt
      361 tttggacggc gcgctggccg gtttggcccg ggcgctggcg ccgcggaccg gtgaaccgga
      421 cgacgatctt cgtcaaaaag ccaaggcccg cctgggtttg ctcgacgaca accacctgcg
      481 gatcgtgccc caggcgcaaa tgaaccgttt ggggaaagtg gggcgttgga agatgttttc
      541 ccggagttat tacggcctga ccatcatcga tgaaaagggc cgtcccgagg cgacctattt
      601 ggccgattgg ttgtttcgcc ccgcgcgggg tcgcttgctc gaacggttgc gagaccgggt
      661 ccttcgaaac gtcctggatc gggaaaaaga acgccgttcg cccagcgctc ttcgacgtca
      721 cgccatcgcc atcgatctgc ggcgcccctt ccgttttctg acggcccccg tcgccggttc
      781 ccggcgccga acggcgaggg ccgcggcggc cgcgatggcc ttcctggccg ggggcgcgac
      841 ggcccagccg gcgatcgacc ccgacctgaa cctcgcgcct ttggcggcgt cggcccggtc
      901 ggtggtggac gccggtctgg tgaaatcccc cgaggggaaa accgccgtcg agcggttcga
      961 gcggtttctc gccgcgggcg gagccggaac cgttcgcgtg ggcaacttcg cctccctgcc
     1021 cgtgcggttc ctggaggaat tggatcgttt gaacgacggt ctggccaagg atcaactgat
     1081 ggtgctcacc cggggaagcg cgggcaacac caccgtcacg ccgctccgtc gggtgggtcc
     1141 gtcgtcgctc tcggaccagg tccttcgagt caccaacatc cccgccgaaa cccaggcccg
     1201 gggcgtgggc ggagaggggg gctatacggt ccgccgcttg tccctcattt acgccgaccg
     1261 actggaattg aaagcccaaa acgtgcggga cgtccttcaa tggcccaaag gccgacggaa
     1321 cttggccctt ccggggtggg acgttttaat cgaccgccaa tttcccatgg gatcggccat
     1381 cgtgtccctg gacgccgtcc ggtccagttt cgaggcgcgt tgggtcatcc acgaatggtt
     1441 gcatcgcgtc ggcgggaaag agcgcgacgc cctgttgggc gaattcatca tcgctccctg
     1501 gctccaggtc acgaccctgg aagtggggct catcgcctat ctgggaggcc ccggcgcgga
     1561 ggcggaaccc gcccgcgtcg ccgcccggga agttttccgc ctcctggaaa aggagctcgt
     1621 ccccgggcgg gagcccctct tgaccccggc gagcactctg cagaccctca cgtcccgggg
     1681 cgatttggat cttcgccggg ccgcgatccg cgcctgggtc aaagcgtcgg gccggccctt
     1741 tttcacctcc gatacgctcg ccctggcccg gaaggccggc ctttcgtcgg acgttctggc
     1801 gtcgcttcag gccgaggaga aatcggccct gcccgaggga accgcccgcc gcgcccaata
     1861 cgaaaacgcc ctggccaagg aacacctggg ttggctgacc gcccaggggg attcgcccac
     1921 ggcccggcgc tatttgcgcg ccgtggcggc caacgcgccg ctcggcccgg acgcccccct
     1981 gacggcggac acggtggttc aactcctccg tcgaatccgg gcccgggagg tggactcgac
     2041 ctttaacaac gccctggcgc ccctggccgc cgaacgtaaa aaacttcccg cctcctcgcc
     2101 cgccgccgcg gcccacgatc gaggcacggc cctggccacg accctgctgg acaatctgct
     2161 ggcccaggtt cggcgggacc accaaaacca ggtcctgccc ctgagcgctc tgagcgccgc
     2221 cggcgcggcg gccctgtccc tgattcttgc ggcgaccggg gtcggaaccc tggacggccc
     2281 ggcggccttc ctggccctac tccccggggc ctggg
//
LOCUS       DAOQLS010000316         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_29594_length_2315_cov_3.251770, whole genome shotgun sequence.
ACCESSION   DAOQLS010000316 DAOQLS010000000
VERSION     DAOQLS010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29594_length_2315_cov_3.251770"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..>2315
                     /locus_tag="PK362_06530"
     CDS             131..>2315
                     /locus_tag="PK362_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74520.1"
                     /translation="MTVAGRALDLSFASADARGRVLDILEPWARLSAFRQKIPGLYYA
                     LVGLWETTVFRYVGLTLIPDLLVRADFPLWVASTSGFLWAVPGFVLAHTLVRWAFHAP
                     EEGWTWKSFSREWSAEWIPNRKLFLGSRPLLGLVLSGVYAFTALLPIDGAAVWALFTA
                     AALHGDWDMSIHRHRSDPVDPQADGEARVRRSLARPEDLSRRWFMSGDFDKLGGKNEL
                     LGKDAIGDLFEWTQGPDILFLRRSLFRETREVVAAAARRGGGRVHRGVGGDELIFEFK
                     GSEPKARAFLNAVVSAVDQHFRGKYAFVAIHRPTLVASEIDALKALPGAMVLERYGAG
                     YRLIVEKKPAGFRSALAAWARLFAGRAPPPPRTDDSLLEEIQGALFSQTDLSPLRFER
                     EGAGPFTLSIGAVNAADTLRVLEETTTEADGHWMKNGRWLPGRRTDLIEWGYRFANDA
                     LNQAKEEGRNRAVILSGPDAIRDAIPPGRVSREKILELRSEVQAAAGTALPENIDHLT
                     GWRTPAAFRSGQRRVRTHIGPFLNYFFIGSYRAGTPLQRRGFHETQSLPNVGHQGGNA
                     LIQSVFGLAEKLFTVDGEPSPRERGARAPPDSGYVAARTDRLASLTSADLDAFEASLP
                     AEIRANPAYDIRPTMFVVTVDERALRSDPSLPDSPYFVTELMTKAVSELLVRHQAQGG
                     PDFWLRALRSIADVEVLEKKDGADAGAPRLIRVTFNPAKIGPLMSH"
BASE COUNT          346 a          796 c          739 g          434 t
ORIGIN      
        1 gacggccccc cgtcgtgccg gggcgcccta cgaattttcc ttcgacgggc ccggcgggcc
       61 ggcccgggtg gccgtcgcgg tcggcccgtc caatccggat cggacggcaa acgccccgct
      121 tgccgtcggc gtgacggtcg cgggccgcgc cctggacctc tcttttgctt ccgccgacgc
      181 gcgcggtcgt gtcctcgaca tcctggagcc ctgggcccgc ctgtccgcgt ttcgccaaaa
      241 aatcccgggg ttgtattacg cgctcgtcgg tttatgggaa acgacggttt ttcgctacgt
      301 gggactcacc ctgattcccg atctcctggt ccgggccgat tttcccctgt gggtcgcttc
      361 gacctccggg ttcctctggg ccgtcccggg gttcgtgttg gcccacaccc tggtccggtg
      421 ggccttccac gccccggagg aaggatggac ctggaagtcc ttctctcggg aatggagcgc
      481 cgaatggatt ccgaaccgta aattgttcct gggctcccga ccgcttttgg ggctcgtctt
      541 gagcggtgtc tacgccttca ccgccttgct gcccatcgac ggcgccgccg tctgggccct
      601 gttcaccgcg gccgccctgc acggggattg ggacatgtcc atccatcggc accggtcgga
      661 ccccgtggat ccccaggccg acggggaggc ccgggtgcgg cggtccctgg cgcgtcccga
      721 ggatttgtcc cggcggtggt tcatgtcggg cgacttcgat aaattgggcg gaaaaaacga
      781 gcttctgggc aaggacgcca tcggcgacct tttcgaatgg actcagggcc ccgacatctt
      841 gtttctgcgc cggtcccttt tccgggaaac ccgcgaggtg gtggccgcgg cggcccggcg
      901 aggcgggggg cgggttcatc gcggagtggg cggggacgag ttgattttcg aattcaaggg
      961 gtcggaaccg aaggcccgcg cctttttaaa cgccgtggtg tccgccgttg accagcattt
     1021 tcggggcaaa tacgccttcg tcgcgatcca ccgtccgacc ctggtggcca gcgaaatcga
     1081 cgccctcaag gccctgccgg gcgccatggt tctggagcgc tacggcgccg gctaccgctt
     1141 aatcgtcgag aaaaaaccgg ccgggttccg atcggccctg gccgcctggg cccggttgtt
     1201 cgcgggccgg gcgcccccgc cgccgcgaac cgacgattcc ttgttggagg aaatccaggg
     1261 cgccctgttc agccaaacgg atttatcgcc cctgcgattc gaacgggagg gcgccgggcc
     1321 tttcacgctt tccatcggag ccgtcaacgc cgccgacacc ctccgcgtgc tcgaagaaac
     1381 gacgaccgag gccgacggcc attggatgaa aaacggtcgt tggttgccgg gccggcggac
     1441 ggatctcatt gaatggggct accgtttcgc caacgacgcg ttaaaccaag ccaaggagga
     1501 gggccgcaat cgcgcggtga tcctctcggg ccccgacgcc attcgggacg ccattccccc
     1561 ggggcgtgtg tcccgggaaa aaatattgga gctccgctcc gaggtccagg cggccgccgg
     1621 caccgccctg cccgaaaaca tcgaccattt gacggggtgg cgcacgcccg cggcctttcg
     1681 ttccggccag cgtcgggtgc gcacccacat cgggccgttc ctcaattact ttttcatcgg
     1741 aagttaccgg gccggcaccc cccttcagcg gcgcggtttc cacgaaaccc agtcccttcc
     1801 caacgtcggc caccagggcg gcaacgccct cattcaatcg gtcttcggat tggccgagaa
     1861 actcttcacg gtggacgggg aaccctcccc ccgggagcgc ggggcccggg cgccgccgga
     1921 ctccggctac gtggccgccc gcaccgatcg cctggcgtcc ctgacctcgg cggacctcga
     1981 cgcctttgaa gcctccctgc cggccgagat ccgcgccaac cccgcttacg acattcgccc
     2041 cacgatgttt gtggtcacgg tggacgaacg ggcccttcga agcgacccgt ccctccccga
     2101 ttcgccctat ttcgtcaccg aattgatgac caaagccgtt tcggaactgt tggttcggca
     2161 tcaggcccag ggcgggcccg atttttggct ccgggccctt cggtccatcg cggacgtcga
     2221 ggttttggaa aagaaggacg gggccgacgc gggcgccccg cgattgatcc gggtcacctt
     2281 taatcccgca aaaatcggac cgctgatgag ccatt
//
LOCUS       DAOQLS010000317         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_29888_length_2303_cov_3.737100, whole genome shotgun sequence.
ACCESSION   DAOQLS010000317 DAOQLS010000000
VERSION     DAOQLS010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29888_length_2303_cov_3.737100"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PK362_06535"
     CDS             complement(<1..1424)
                     /locus_tag="PK362_06535"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNC74521.1"
                     /translation="MSKSDRIVIFDTTLRDGEQSPGASLNGEEKRQAAHQLAELGVDV
                     IEAGFPVSSPGDFEAVKAIAREVRGPEIAGLARCVKGDIDACWGAIKHAKKPRIHTFI
                     ATSDLHLQSKLKMTRSQVLDRAVECVRYAAKLTGNVEFSAEDAVRSDFDYLCQVVEAV
                     IDAGATTVNIPDTVGYGIPSDWGARIGQIIARVPNIRRAVVSVHCHNDLGLAVANSLA
                     AVQNGARQVECTVNGIGERAGNASLEEIVMALRTRKDLFNVTTGVRTHEIAKTSRLIS
                     KLTGIVVQPNKAVVGANAFAHSSGIHQDGVIKSRTTYEIMNPEDVGVEESALLLTARS
                     GRNGVNTRLKHLGFHLAAPALETLFEKFKVLADKKKYVFDDDLIALVEEEGRAKAPEV
                     FSMDYLNTTSGTGIVPTATVRLKKKDAVFQEAACGDGPVDAAYRAMDKITGLRPELLD
                     YALRSVSSGTDAQGDVVVKVADKGL"
     gene            complement(1482..>2303)
                     /gene="pssA"
                     /locus_tag="PK362_06540"
     CDS             complement(1482..>2303)
                     /gene="pssA"
                     /locus_tag="PK362_06540"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNC74522.1"
                     /translation="ASTAPVPPVRRGIYLLPALFTVGNMALGFFALIKSVGHEFSAAA
                     TAILFGHVLDIFDGAVARLTRTSSRFGIELDSLADWMTFCIAPSFLMYEMVLKDNRSW
                     GFPIALLFVICGALRLARFNLKAQMGEAPPSFFTGLPTPAAGGVLAIFALLYDVMELG
                     KPIRSFKLVMNQVPVFYEVVPAVMLLLSLLMVSDVRYAKFRTQNLLRPRSMRALVLTI
                     LAVLMIWVYPQNTIFILYVSYIVWGLVDYFLGRSLRRPSTPHASDSEEHVDHYGK"
BASE COUNT          478 a          693 c          697 g          435 t
ORIGIN      
        1 agccccttgt cggcgacctt gaccaccacg tcgccttggg catcggtccc ggaggagacc
       61 gaccgaaggg cgtaatccaa cagttccggg cgcaggccgg tgatcttgtc catggcccga
      121 taagccgcgt ccaccgggcc gtccccgcag gccgcttctt ggaacaccgc gtctttcttc
      181 ttcaaacgca cggtcgccgt cggaacgatc cccgtgccgg acgtcgtgtt caggtaatcc
      241 atggaaaaaa cttccggcgc cttggcgcgg ccttcctcct ccaccagggc gatgagatcg
      301 tcgtcgaaaa cgtatttctt cttgtccgcc agaactttaa acttttcaaa cagcgtttca
      361 agcgccgggg ccgccaaatg gaaccccaaa tgcttaaggc gcgtatttac cccgttgcgc
      421 cccgaacggg cggtcaggag caaagcgctt tcctccacgc ccacgtcctc ggggttcatg
      481 atttcgtagg tggttcggga cttgatgacc ccgtcctggt gaatgccgga ggagtgggcg
      541 aaggcgttgg ccccgaccac ggctttgttc ggctgaacca cgatcccggt cagcttcgaa
      601 attaagcggc ttgttttggc gatttcgtgg gtgcgaacgc cggtcgtcac gttgaacaaa
      661 tccttgcggg tgcgcaaggc catcacgatt tcttccaggg aggcgtttcc cgcgcgttcg
      721 ccgatgccgt tgacggtgca ttccacctga cgggccccgt tttgaacggc ggccaaagaa
      781 ttggccacgg ccaatcccag atcgttgtga cagtggaccg acaccacggc gcgtcgaatg
      841 ttgggcaccc gggcgatgat ttggccgatg cgcgcccccc agtcggaggg gatcccgtag
      901 cccaccgtgt ccgggatgtt tacggtggtg gccccggcgt cgatgaccgc ttcgaccacc
      961 tggcaaagat agtcgaaatc cgatcggacg gcgtcttcgg cggaaaactc cacgttgccg
     1021 gtcaacttgg ccgcgtaacg gacgcattcg accgcccgat cgagaacctg ggagcgcgtc
     1081 atttttaatt tactctgcag gtgaaggtcg gaggttgcga tgaacgtgtg gatgcggggt
     1141 tttttggcgt gcttgatcgc gccccaacaa gcgtcgatgt cgcctttgac gcaccgggcc
     1201 aacccggcga tttcgggtcc ccgaacttcg cgggcgatgg ctttgacggc ctcaaaatcg
     1261 ccggggctcg ataccgggaa gccggcctcg atgacatcga cccccaattc ggccaattga
     1321 tgggcggcct gacgcttttc ttctccgttc aggctggcgc ccggggattg ctccccgtcc
     1381 cgcagggtgg tgtcaaaaat gacgatacgg tctgacttcg acataaacgg tctcctcaaa
     1441 aatcggtggc cccggcccgt cgcgattcgc cgggcgaaac gctatttgcc gtaatgatcg
     1501 acgtgttctt cggaatccga tgcgtggggg gtggagggtc gccggaggga gcgaccaagg
     1561 aaataatcga cgagacccca cacgatgtag gacacgtaaa ggatgaaaat cgtgttttgg
     1621 gggtaaaccc aaatcatgag gacggccaat atcgtcaaca ccagcgcccg catgctgcgg
     1681 ggccgcaaca agttctgcgt ccggaatttg gcgtaacgaa cgtccgacac catcagaagg
     1741 gacaacagga gcatcaccgc cggcaccact tcgtaaaaca ccggcacctg attcattacc
     1801 aatttaaaac tccggatggg ttttcccaac tccatcacat cgtagagcag ggcgaagatg
     1861 gccaacacgc cgccggccgc cggcgtcggc aatccggtga agaaggaggg cggcgcctcc
     1921 cccatctggg ccttgaggtt gaaccgggcc aggcggagag cgccgcaaat cacgaacagg
     1981 agggcgatcg ggaaacccca ggaccgattg tctttcaaca ccatttcata catcaggaag
     2041 gagggcgcga tgcaaaaggt catccaatcg gcgagggaat ccaattcgat tccgaaccgg
     2101 ctggacgtcc gggtgaggcg cgccacggcg ccgtcaaaga tatccaagac gtggccgaac
     2161 aaaatggccg tggccgccgc gctgaattcg tggccgacgg atttgatcaa cgcgaaaaac
     2221 ccgagcgcca tattcccgac ggtaaacaag gccggcagca gatagattcc ccgccgaacc
     2281 ggcggcacgg gcgcggtgga ggc
//
LOCUS       DAOQLS010000318         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_29941_length_2301_cov_3.187444, whole genome shotgun sequence.
ACCESSION   DAOQLS010000318 DAOQLS010000000
VERSION     DAOQLS010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29941_length_2301_cov_3.187444"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1261)
                     /locus_tag="PK362_06545"
     CDS             complement(122..1261)
                     /locus_tag="PK362_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC74523.1"
                     /translation="MDTHPAARTALNSERPAPPRLIFWETTAGCNLRCVHCRRLDVLD
                     QVSETDLSTAFAREMISDIASYCKPILVLSGGEPLFRPDILTIADHARSEGLRVALAT
                     NGTLVTAEKARDIHKAGVARVSISLDGAVAATHDRFRGAGNFERALAGFRHLKAQGVS
                     LQINMTVTRYNAEELPRLYDLCRAMGADALHLFMLVPVGCGVQIADSDMLPSAEYEKW
                     LNWFYEREREQRIEMKATCAPHYFRIVRQRSKEIGGLPPSHHRQEAKGATHPHQALHQ
                     TTKGCLAGQGVCFISHKGEVFPCGYLPISVGSVRTTRFKDIWELSPVFQDLRNPALLE
                     GKCGACSFKYVCGGCRARAYYAHGDALAEEPHCVYVPDGYQGDLA"
     gene            1435..>2301
                     /locus_tag="PK362_06550"
     CDS             1435..>2301
                     /locus_tag="PK362_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74524.1"
                     /translation="MTLGFTAGTVTLALGTRGTLTFDAAGRPRALYENDRYIARGWDG
                     RCLEKKWVSAPGGLFHRRVRSLEAGERAALRDRFRLLLEGFQRDLPALRRARRFRCLD
                     RGRPVRDGGERAAAFVAAARSKLAAEWDTEAADFHRTYRPIGVLPPDQYLSLVLQVTE
                     GCAWNRCSFCDFYAGQPFRVKSPAEFDRHLEDVERLFGPALAGRCSLFLGSANALDVP
                     PDVLIPSVRRAAERFPALARPQDDGVGGVYAFGEATRLARWSADDLRRLRRAGLRRAY
                     LGVETGDPDLRRK"
BASE COUNT          378 a          739 c          776 g          408 t
ORIGIN      
        1 gggaaccggg cctcgagggc caccgcgtcg aaggcgggcg gcgccggagg cgcttcggtc
       61 ctttggcaac cgacggcgat catccccgac aaaatcaaga tttggaggac gccgcggccg
      121 gtcacgccaa atccccttgg taaccatcgg gcacgtagac gcaatggggc tcctccgcca
      181 gggcgtcgcc gtgggcgtaa taagcccggg cccggcagcc gccgcagacg tatttgaacg
      241 aacaggcccc gcacttgcct tccagcaggg cggggtttcg caaatcctga aagacgggcg
      301 aaagctccca aatgtccttg aaccgggtcg ttcgaacgct cccgaccgaa atcggcagat
      361 acccgcaggg gaagacctcc cctttgtggg aaataaagca aacgccctgg ccggccaggc
      421 accccttggt cgtctggtgg agcgcctggt gggggtgcgt ggcgcccttg gcctcctggc
      481 ggtggtggct ggggggcaga ccgccgattt ccttggaccg ttggcgcacg atgcggaaat
      541 agtggggggc gcaggtggct ttcatctcga tccgctgttc ccgctcccgc tcgtaaaacc
      601 agttgagcca cttttcgtac tccgcggagg gcaacatgtc cgaatcggcg atttgcacgc
      661 cgcagcccac ggggaccagc atgaagaggt gcagggcgtc ggcccccatc gcccggcaca
      721 ggtcgtacag gcgcggcaac tcctcggcgt tgtagcgggt gacggtcatg ttgatctgaa
      781 gggacacgcc ctgggccttg agatgccgaa acccggccag ggcgcgctcg aaattgcccg
      841 cgccgcgaaa acgatcgtgg gtcgcggcca cggccccgtc cagggagatg ctcacccggg
      901 ccacgccggc cttgtggatg tcccgggcct tttcggcggt caccaacgtt ccgttggtgg
      961 cgagggccac gcggagtcct tcgcttcggg cgtggtcggc gatggtcaga atgtccgggc
     1021 ggaagagcgg ttcgccgccg gaaaggacca aaatgggttt gcagtaggag gcgatgtccg
     1081 aaatcatctc ccgggcaaaa gccgtggaca aatcggtttc ggacacctgg tccaagacgt
     1141 ccaggcggcg gcaatgaacg caacgcagat tgcagcccgc cgtggtttcc caaaagatga
     1201 ggcgcggcgg cgccgggcgt tcgctgttta acgccgttcg ggcggcggga tgggtgtcca
     1261 tagccgtggg gaaggtcacg gagtaagtat atccgtccgg tgacgaacat ccatgacgct
     1321 cgtcatgttt cgcccgggga caagatggta cactccctct tcaggagctt cccgacctgg
     1381 gaagcttttt ttattatggc caacgtccca acccgatccg tttcacccgc cgccgtgacc
     1441 ctgggattca ccgccgggac ggtgactctg gccctgggga cccggggcac gctcaccttc
     1501 gacgccgccg ggcggccccg ggccctctac gaaaacgacc gctacatcgc ccgggggtgg
     1561 gacggccgct gcctcgagaa aaagtgggtt tccgctccgg gcgggctttt ccaccgacgg
     1621 gtgcgctccc tggaagcggg agaacgcgcg gcccttcgcg atcgatttcg ccttcttttg
     1681 gaaggttttc agcgggacct gcccgccctt cgacgggcgc gccgattccg gtgcctcgac
     1741 cggggacgcc ccgtccggga cgggggcgaa cgggccgcgg ccttcgtcgc ggcggctcga
     1801 tccaaattgg cggcggaatg ggacaccgag gccgcggact ttcaccgaac ctaccgtccc
     1861 atcggcgtcc tgccgccgga tcaatacctt tccctggtgc ttcaagtcac cgaagggtgc
     1921 gcttggaacc ggtgctcttt ttgcgatttt tacgcgggcc aacccttccg cgtcaaatcc
     1981 ccggcggagt tcgaccggca cctggaggac gtggaacgcc tcttcggccc cgcgttggcg
     2041 ggacggtgtt cgctttttct cgggtcggcc aacgccctgg acgtcccccc ggacgtgtta
     2101 attccctcgg ttcggagggc ggcggaacga ttccccgccc tggcccggcc ccaggacgac
     2161 ggcgtggggg gcgtttacgc tttcggcgaa gccacgcgcc tggcgcgttg gtccgccgac
     2221 gatttgcggc ggcttcgacg ggctgggctt cgacgggcct atctgggcgt tgaaacgggg
     2281 gaccccgacc ttcgtcgaaa a
//
LOCUS       DAOQLS010000319         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_29956_length_2300_cov_4.109131, whole genome shotgun sequence.
ACCESSION   DAOQLS010000319 DAOQLS010000000
VERSION     DAOQLS010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29956_length_2300_cov_4.109131"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK362_06555"
     CDS             <1..193
                     /locus_tag="PK362_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC74525.1"
                     /translation="KTCTEAGLYSAWGVPAVVWGPGRSTGNVHRPDESIAERDLAKAV
                     DFYEALLRRWIQKNQEKAP"
     gene            190..1221
                     /locus_tag="PK362_06560"
     CDS             190..1221
                     /locus_tag="PK362_06560"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF016822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HNC74526.1"
                     /translation="MTAPSNFVLRSARPDDLDDVYRLARHLDSYNLPADRSRLRVLLA
                     DSVRSFAGRPPSHPREKFLFLLEDRTLGRVVGSSLVIAQHGTPGLPHLFLQSFVERRR
                     SRTLGRSVDHRCLKLGWTDRGPTELGGLVLLPSHRGRPEKLGHWLSYVRLLFIAARRR
                     RFRPRLLAEYLPAFAKNGVSPFWEYFGKKFTGLDYRTADRLSIDNKEFIVSLFPSGTL
                     YQDFFPEEVVRYLGQVGNPSLPAAQLLRKAGFAYCSQIEPFDGGPYFEADTDRVPLVR
                     RARRTAWAVNSTPAETRRLVLVDRGGEVRARMAPAALGGGRLRLPPTAAASLDLRPGE
                     TVWSVGVDR"
     gene            1218..>2300
                     /locus_tag="PK362_06565"
     CDS             1218..>2300
                     /locus_tag="PK362_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC74527.1"
                     /translation="MKRDFGPLSFRGAYVRGRFVPPTEGDRFLSEDPGDAARPVGEAR
                     GSTLLVADAVAAARSAAPAWREESLAQRIAILRRYQKKLAGHGDRLAWVMSRETGKPL
                     QESRDEVAAMVAKVDLTVTRGLPLVADRRAPLDGGSRSVLRYRPRGVVGVIGPFNVPG
                     HLPNGQIVPALLTGNTVVFKPSEFTPFVGQVLAELFDEAGFPPGVFNLVQGGGEVGAA
                     LAAEPGVNALFFTGSAATGEKVRALSDRCPDRLLALELGGKNAALILPDAPWEAALGQ
                     VVHGAFATAGQRCSSTSRLFVHKKIARRFIRDLMARAAALTVGYFTESPGLGPLIDGR
                     AVQRFEAAQESAEKRGFATLKRGVPAP"
BASE COUNT          333 a          786 c          740 g          441 t
ORIGIN      
        1 aaagacctgc accgaggccg gcctttacag cgcctggggc gtccccgccg ttgtttgggg
       61 ccccggccgg tcgaccggca atgtccaccg ccccgacgaa tccattgccg agcgggacct
      121 tgccaaggcc gtggattttt acgaagctct tttacggcga tggatccaaa aaaaccaaga
      181 aaaagccccg tgaccgcccc gtcgaatttt gtccttcgaa gcgcgcgtcc cgacgatttg
      241 gacgacgttt atcggttggc ccgccatttg gattcttaca atttgccggc ggaccggtcc
      301 cgtctgcggg tcctgttggc cgattccgtc cgctccttcg ccgggcgccc gccgtcccac
      361 ccccgggaaa aattcctgtt cctgctggag gaccgaaccc tgggccgggt tgtcggatcc
      421 tccctcgtga ttgcccaaca cggaacgccg ggactccccc accttttcct tcaatccttc
      481 gtcgagcgcc gccggagccg caccttgggc cggtcggtgg accaccggtg cttgaaattg
      541 ggttggaccg accgggggcc cacggaattg ggcggcttgg ttctcctgcc ctcccaccgg
      601 ggccggccgg aaaaattggg ccattggctt tcctacgttc ggttgttgtt catcgcggcc
      661 cgccggcggc gcttccgccc ccggttgctg gccgaatacc ttcccgcctt cgccaaaaac
      721 ggcgtcagcc ccttttggga atacttcggg aaaaaattca ccgggttgga ttaccgaacg
      781 gcggaccggc tgagcatcga caacaaagaa ttcatcgtgt ccctctttcc ctccggcacc
      841 ctctaccagg atttttttcc ggaggaggtc gttcgctatc tgggacaggt gggaaatccc
      901 tccctgccgg cggcccaact gctccgcaag gcgggcttcg cctactgctc ccaaatcgaa
      961 ccctttgacg gcggccccta ttttgaagcc gacaccgacc gcgtgccgtt ggtgcgccgg
     1021 gcccggcgga cggcttgggc ggtaaattcg accccggccg aaacccggcg cctggtgttg
     1081 gtggatcggg ggggcgaggt ccgggcccgg atggcccccg ccgccttggg cggcggacga
     1141 cttcggcttc cccccaccgc ggcggcgtct ctcgacctcc gccccgggga aacggtctgg
     1201 tccgtggggg tcgatcgatg aagcgcgatt tcggccccct gagttttcgc ggggcctacg
     1261 ttcggggccg ttttgttcct cccacggagg gcgatcgctt cttgagcgag gacccgggcg
     1321 acgcggcccg tcccgtgggc gaagcccggg ggtcgacctt gttggtggcc gacgccgtgg
     1381 ccgccgcgcg gtccgcggcg cccgcctggc gggaggagtc cctcgcccag cggatcgcga
     1441 ttcttcgtcg ttatcaaaag aaactcgcgg ggcacggcga ccggctggcc tgggtcatgt
     1501 cccgggaaac cggcaaacct cttcaggaat cccgggacga ggtggcggcc atggtcgcca
     1561 aggtggattt gaccgtgacc aggggtctgc cgctcgtggc cgaccgtcgc gcccctttgg
     1621 acggcgggtc ccgaagcgtc cttcgttacc gtccccgggg cgtggtgggc gtcatcggtc
     1681 ccttcaacgt ccccggccat ttgcccaacg gtcaaatcgt cccggcgctc ctgaccggga
     1741 acaccgtcgt gttcaagccg tcggaattca cgcccttcgt gggccaggtc ctggcggagt
     1801 tgttcgacga agcgggattt ccgcccgggg tgttcaacct cgttcaaggc gggggcgagg
     1861 tgggcgcggc cctggccgcc gagcccggcg tgaacgccct gtttttcacc ggttccgccg
     1921 ccacgggcga aaaagtccgc gccctgagcg accgatgccc cgatcgtttg ctcgccttgg
     1981 aactgggcgg caagaacgcg gcgctgatcc tccccgacgc gccgtgggag gccgccctgg
     2041 ggcaggtggt gcacggcgcc tttgccaccg cgggccagcg ttgttcgtcc accagccgtt
     2101 tgttcgtcca caaaaaaatc gcccgccgat tcatccgcga tttgatggcc cgcgccgcgg
     2161 cgttgaccgt cggctatttc accgagtccc cgggcttggg gcccctgatc gacggacggg
     2221 ccgttcaacg ttttgaagcc gcccaggaat cggcggaaaa acggggtttc gccaccttga
     2281 agcgcggagt tcccgcgccc
//
LOCUS       DAOQLS010000320         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30014_length_2298_cov_3.540348, whole genome shotgun sequence.
ACCESSION   DAOQLS010000320 DAOQLS010000000
VERSION     DAOQLS010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30014_length_2298_cov_3.540348"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PK362_06570"
     CDS             <1..257
                     /locus_tag="PK362_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74528.1"
                     /translation="RPLVYADQGPYWIAAGEAVLKKAVEDLSEGGRRGREVRQLVHPG
                     AFGGAFRILIQGKNVEGIGLRAEIAARLQRLGAGDLVVRG"
     gene            complement(218..1021)
                     /locus_tag="PK362_06575"
     CDS             complement(218..1021)
                     /locus_tag="PK362_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC74529.1"
                     /translation="MGLKSWGGALAILAAGLSIAGAEPFDVPVPGRTENDPLLLQGDF
                     TPPRTANAPVLVCLHGLGSTRAEWQPLIDAARKKGWGAYAYDARGHGRSQSTLAGDTI
                     AHDSRRDRTPAFWKAMPGDLIRVLDALEKQKNVAPKQRILVGASLGANVCLLGAGSSP
                     AAGVIALSPGIEYAGLNVESAMAGLPAPALLVSAKTDLYAHASAERLLSLAGRPGFVR
                     WTALDGGTARGDHGTQLFDGKMEERLLEWIASVTKTSQPRTTKSPAPKR"
     gene            complement(1012..1851)
                     /locus_tag="PK362_06580"
     CDS             complement(1012..1851)
                     /locus_tag="PK362_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNC74530.1"
                     /translation="MKKPAGPLLRSLGALWIAACLVAMALTVRYMKGAVRNAFVLRSA
                     GVSQLAQANLQEFLDRHTRDLGDLAQNLGEAPPNGQRDFEDRAARVLPENPAFAALNF
                     FNRDFILTWGFPYAANRTAEGLNLKTHFAGLAAARRAIERHGPAASGLVDLLQGESGV
                     LLYAPVFEGDTWRGLIEGALQIPRLAARFIAPLFGPRFRFMIIDERRGEELYSNMREV
                     DHARTPAYDAYFTLNVADRIWWVVLHPVSPPPTLALVVGALMVELAVGAGVLILAGRR
                     KWD"
     gene            complement(1848..>2298)
                     /locus_tag="PK362_06585"
     CDS             complement(1848..>2298)
                     /locus_tag="PK362_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74531.1"
                     /translation="ALLREGRKEFPNSPFFHFAELLILDEARDWSALRIGAEDFLARI
                     RRADRYYGKGREHMGLFALGNSYLIEGDAGRALDIYEDVLANHHQQDRWITLTYLHRG
                     MAYDLRGRRDLAVADYNTVLDRRDVWELHDKARALLKHPYQPPRKKR"
BASE COUNT          412 a          747 c          757 g          382 t
ORIGIN      
        1 tccggcccct ggtctacgcc gatcaaggcc cctactggat cgcggccggg gaagccgttt
       61 taaaaaaagc ggtggaggat ttgtcggagg ggggacgacg ggggcgggag gtgcgtcagt
      121 tggttcaccc cggcgccttc ggcggggctt tccggatcct gattcaaggc aaaaacgtgg
      181 agggcatcgg cctgcgggcc gaaatcgcgg cacggcttca gcgtttgggc gcgggcgatt
      241 tggtcgttcg cggctgagac gttttcgtca cgctcgcgat ccattccaat agccgctctt
      301 ccatctttcc atcaaaaagc tgggtcccgt ggtctccccg ggcggtcccg ccgtccaggg
      361 ccgtccagcg aacaaacccc ggccgccccg cgagggacaa cagccgctcg gcgctggcgt
      421 gggcgtataa atccgttttg gcggagacca gaagcgccgg cgccggcagg ccggccatgg
      481 cgctttccac attcaatccg gcgtattcga ttcccggcga cagggcgatg acgcccgccg
      541 cgggcgagga ccccgcgccc agaaggcaga cgttggcgcc caggctggcg cccaccagga
      601 tccgttgctt gggcgcgacg tttttttgtt tttccaaggc gtccaagacc cggatcaagt
      661 ccccgggcat ggccttccaa aacgccggcg tccggtcccg tcggctgtcg tgggcgatgg
      721 tgtccccggc caaggtgctt tggcttcgcc cgtggccccg ggcgtcgtag gcgtaggcgc
      781 cccagccctt tttccgcgcg gcgtcgatca ggggttgcca ctccgcccgg gtgcttccga
      841 gcccgtgaag gcacaccagg acgggcgcgt tggccgtccg ggggggagta aaatcgcctt
      901 gaagcaaaag cgggtcgttc tccgtccggc cggggacggg aacgtcgaag ggctccgccc
      961 ccgcaatgga aaggcccgcc gccaaaatcg ccagcgctcc gccccaggat ttcaatccca
     1021 ttttcgcctc ccggccaata tcagaactcc cgcgcccacg gccaattcca ccatcaaggc
     1081 cccgaccacc agcgccaacg tcgggggcgg cgagaccggg tgcagaacca cccaccaaat
     1141 ccggtccgcc acgttcaagg tgaaataagc gtcgtaggcc ggggtccggg cgtggtcgac
     1201 ctcccgcatg ttggaataga gctcctcgcc ccgccgttcg tcgatgatca tgaaccggaa
     1261 ccgcgggccg aagagcggcg caatgaaccg cgcggccaac cgggggattt gaagggcccc
     1321 ctcgatcagc ccgcgccagg tgtcgccctc aaaaaccggg gcgtaaagca acacgcccga
     1381 ttcgccctga aggagatcca ccagtccgga ggccgcgggc ccgtggcgtt cgatggcccg
     1441 tcgagcggcg gccaatccgg cgaaatgcgt tttaaggttc aacccctcgg cggtgcggtt
     1501 ggccgcgtag gggaaccccc aggtcaggat gaaatcgcgg ttgaaaaaat tgagggcggc
     1561 gaaggcgggg ttttcgggga gaacccgggc cgcccggtct tcgaaatccc gctgaccgtt
     1621 cggcggggcc tcccccaaat tttgggccag gtcgcccagg tcccgggtgt ggcggtccag
     1681 aaactcctga agattggctt gggccagctg ggacaccccg gcggaccgga gaacgaaggc
     1741 gttccgcacg gcgcccttca tgtagcggac cgtgagagcc atggcgacga ggcaagcggc
     1801 gatccaaagg gcgcccaacg accgaagcag gggaccggcg ggttttttca acgtttttta
     1861 cggggcggct ggtacgggtg tttcagcaac gcccgggcct tgtcgtgaag ctcccacacg
     1921 tcgcgtcgat ccagaacggt gttgtaatcg gccacggcca gatcccgccg tccccgaagg
     1981 tcgtaagcca ttccccggtg aagataagtg agggtgatcc atcggtcctg ttgatggtgg
     2041 ttggccaaca cgtcctcgta aatgtccaag gcccgccccg cgtcgccctc gatcaaatag
     2101 gaattcccca gggcgaaaag ccccatgtgt tcccggcctt tcccgtagta acgatcggcc
     2161 cggcgaatgc gggccaggaa atcctcggcg ccgatccgaa gggccgacca gtcccgggcc
     2221 tcgtccaaaa tcaacagctc cgcgaaatgg aagaaggggc tgttggggaa ttctttgcgc
     2281 ccctcccgga gaagggcc
//
LOCUS       DAOQLS010000321         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30051_length_2296_cov_4.524766, whole genome shotgun sequence.
ACCESSION   DAOQLS010000321 DAOQLS010000000
VERSION     DAOQLS010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30051_length_2296_cov_4.524766"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PK362_06590"
     CDS             <1..786
                     /locus_tag="PK362_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74532.1"
                     /translation="PLQGYDGDLMEPFLSHDNQYLFFNNSNVPGEKTDLFYARRTPDG
                     GFRFVGPVKGANLPPPVLDGVASMDRNHRFYFTTTRDYANSFLTIYSGNFTKEVLTDV
                     APVLGDFSRGKLKWVTMDVEVSEDGNTIYCSDAYFPSLEKGVPGLSDIKIARKSPDGR
                     FLLLPDTDAILRNVNTPDLEYAPSISPDERELFFTRLRLKDLHTAILRAKRRSAADPF
                     DVPEALAAVTGFVEAPTLAPDGKTLYYHKKDGNRFALYQVTRE"
     gene            924..1232
                     /locus_tag="PK362_06595"
     CDS             924..1232
                     /locus_tag="PK362_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74533.1"
                     /translation="MITCRFESAASRQSAAVPLNETPRLIDSSDVIGSDHGGGDRIEA
                     RLVEKGRVVLVEIQTAAQQKGGPSNSATQFHYVDSTLLPRRIIVEGMILKGSVVVEAK
                     "
     gene            1393..1614
                     /locus_tag="PK362_06600"
     CDS             1393..1614
                     /locus_tag="PK362_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC74534.1"
                     /translation="MWDGDVSVKGIVKWFNASKGFGFITPEGGQEDVFVHFSAIQGDG
                     YKSLNDGQAVELEVTKGPKGLQAANVRPA"
     gene            1698..>2296
                     /gene="kdsA"
                     /locus_tag="PK362_06605"
     CDS             1698..>2296
                     /gene="kdsA"
                     /locus_tag="PK362_06605"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNC74535.1"
                     /translation="MASSNVVSIGPLRLANDRPLALIAGPCVIESEELTLRVAKSLKE
                     TAAKLRIPFIFKCSYDKANRSSIGSYRGVGVREGLEILARVKREVSVPVLTDIHAMED
                     IAPVAAVVDVLQIPAFLCRQTDLVVGAIKSGRVVNVKKGQFLSPWEAANIVEKARAAG
                     GERLMLTERGATFGYNNLVVDMRSLEVMKGHGVPVVFDAT"
BASE COUNT          454 a          694 c          689 g          459 t
ORIGIN      
        1 ccccttcagg ggtacgacgg ggacctgatg gaaccctttc tctcccacga caaccaatac
       61 ctgttcttca acaactccaa cgttccgggg gagaagaccg accttttcta cgcccggcgc
      121 acgcccgacg gcgggttccg gtttgtgggt ccggtgaagg gcgccaactt gcccccgccc
      181 gttttggacg gggtggccag catggaccgg aatcaccggt tttacttcac caccacgcga
      241 gactacgcga acagctttct gaccatttac agcgggaatt tcacgaaaga agtcctgacg
      301 gacgtcgccc cggtcctggg ggatttctcc cgcgggaagc tcaagtgggt caccatggac
      361 gtggaggtga gcgaagacgg gaacacgatt tattgctcgg acgcctattt ccccagcctg
      421 gaaaagggcg tgcccggcct ctcggacatc aagatcgccc ggaagagccc cgatggccga
      481 ttcctgcttc ttcccgacac cgacgcgatc ctgcgcaacg tcaacacgcc ggacctggaa
      541 tacgcgccct ccatctcccc cgacgaacgg gagcttttct tcacccggct ccgcttgaag
      601 gacctccaca ccgccattct ccgggccaaa cggaggagcg ccgccgatcc ctttgacgtc
      661 cccgaagccc tcgccgccgt cacggggttt gtggaggcgc ccacgttggc cccggatggc
      721 aagacgctgt actatcacaa gaaggacggc aatcgctttg ccctctacca ggtgacgcgc
      781 gagtgatcgg tgccttaaga aaatggcggt ggggcgcggc tttgccgaaa cggggcatcg
      841 gcgtcctttt gctcgttgcc ggcgctctcg ggtacgccgg cgcgcaggca cggaaccaga
      901 ttaaacggga ggagaaaacc aaagtgatca catgccgttt tgaatccgcc gcgtcgcgcc
      961 aaagcgcggc ggttccgctg aatgaaaccc cgcggttgat cgattcttcc gatgtgatcg
     1021 gttccgatca cggcgggggc gatcgcatcg aggcgcgtct ggtcgaaaag ggccgggtcg
     1081 ttttggtcga gatccagacg gccgcgcaac agaagggggg gccgtcgaac agcgccaccc
     1141 aattccatta cgtcgattcc acccttttgc cgcgccggat tatcgtcgaa gggatgatcc
     1201 tgaaagggtc ggtggtggtg gaggccaaat agtacttggc ttttaaaggg aaacatgcca
     1261 tactaagccc cgtaagagtt gaagttcgac ttccggtatc tctcagtgga acatcatggc
     1321 gatccgcggg tccgttaggc ccgtggattt ttttatgcgg ttcgctgtgg aagacgcttc
     1381 gcaatctgtc ttatatggga tggtgatgtt tcagtgaaag gcatcgtgaa atggttcaac
     1441 gcctccaagg ggttcggttt catcaccccg gaaggcggcc aagaggacgt gttcgtccac
     1501 ttctcggcca ttcagggcga cggttacaag tccttgaatg acgggcaggc ggtggaattg
     1561 gaagtgacca aaggccccaa agggctccag gccgctaacg ttcgaccggc gtaattttcg
     1621 cctcttgttc aagcgcgccc ccgccaggga aacccggcgg gggcgtgtct tttttatgta
     1681 gaatagtccg ctccaccatg gcttcctcca acgttgtatc catcggcccc cttcgcctgg
     1741 ccaacgatcg ccccttggcg ctgatcgcgg gcccctgcgt catcgaatcc gaagagttga
     1801 ccctgcgggt cgccaaaagc ctcaaagaga ccgccgccaa gctccgaatc cccttcattt
     1861 tcaagtgttc ctacgacaag gccaaccgct cctccatcgg atcctaccgg ggcgtggggg
     1921 tgcgtgaagg cctggaaatt ttggcccggg tcaaacggga ggtgagtgtt cccgtgttga
     1981 ccgacatcca cgccatggaa gacatcgcgc ccgtggccgc ggtggtcgac gtcctgcaaa
     2041 tcccggcttt cctctgccgc caaacggacc tcgtcgtcgg ggcgattaag tccggccgcg
     2101 tggtgaacgt taaaaagggc caattcctgt ccccctggga agccgccaac atcgttgaaa
     2161 aagcccgggc cgccggcggc gaacgattga tgctcacgga acggggcgcg accttcggtt
     2221 acaataattt ggtggtggac atgcgcagct tggaagtcat gaaaggccac ggcgtgccgg
     2281 tggtgttcga cgcgac
//
LOCUS       DAOQLS010000322         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30116_length_2293_cov_3.924039, whole genome shotgun sequence.
ACCESSION   DAOQLS010000322 DAOQLS010000000
VERSION     DAOQLS010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30116_length_2293_cov_3.924039"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PK362_06610"
     CDS             <1..792
                     /locus_tag="PK362_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNC74536.1"
                     /translation="PTKTEQTDLLQAMFGRNGESPVVVVAPKSPAECFEMAIEAVRLA
                     LTHMTPVIFLSDGYLSNSAEPWKLPDISKFAKIVVNHPTTWKGPFTPYARDPKTLARA
                     WAKPGTPGLQHRIGGLEKNDVTGAVSYDPDNHEKMGQIRAEKVARVADDIAPLAVRGA
                     DRGRLLVLGWGSTYGPIYQAVSEVTEAGRAVSQAHLTHLNPFPKNTGEVLSKFEKVLV
                     PELNSGQLLMLLRHRFPRVDFVGYNRVRGVPFYVSEVREAIEAAL"
     gene            789..1826
                     /locus_tag="PK362_06615"
     CDS             789..1826
                     /locus_tag="PK362_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNC74537.1"
                     /translation="MTEVPVPSGAEKRPTFTKNDFVSGAEVRWCPGCGDYSILGSMQQ
                     TLAKFDIPRENYVFVSGIGCSSRFPYYMNTYGFHTIHGRAPTIATGLRCVNPDLSIWV
                     ITGDGDGLSIGGNHLLHAMRRNLNFNILLVNNQIYGLTKGQYSPTSPTGKMTKSSPYG
                     SIDHPINPLCIAIASEATFIARTLDSDPRHMSATLERAMKHKGIAFVEVYQNCIIFND
                     KAHGPVTGRETRDDRMVYLEHGKPLLFGKNKSKAIRLNGLYPEMADYDPTNTRGLLIH
                     DETDPRPNHAYLLTQLTYPDFPVPFGVFRAVEKPTYDDLMNQQIRDIVAKKGKGSLEK
                     LIYSGDTWRVQ"
     gene            1823..>2293
                     /locus_tag="PK362_06620"
     CDS             1823..>2293
                     /locus_tag="PK362_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC74538.1"
                     /translation="MSANPCPFCGHENLPGDDRCGQCLHSLMQRQIPSARRVGDEFRD
                     ALLKEPVSELLTGVDLLVCSPKDSLEKILKIFQKEKKGCVLVYEKKKMVGILSNRDLL
                     LRAAGPGDDLSKLTVEDVMTRNPSYVRPEDPIAFAVNKMSVGGYRHVPVIRADGT"
BASE COUNT          484 a          811 c          613 g          385 t
ORIGIN      
        1 cccacgaaaa ccgagcaaac cgatctcctg caggccatgt tcggacggaa cggggagtcc
       61 ccggtggtcg tcgtggcccc caaaagcccg gccgagtgtt tcgagatggc cattgaagcg
      121 gtccgcctcg ccttgacgca catgaccccg gtgatcttcc tttccgacgg ctacctgtcc
      181 aacagcgccg aaccctggaa gcttcccgac atctccaaat tcgccaaaat cgtggtgaac
      241 catccgacga cctggaaagg cccgttcacc ccctacgccc gggacccaaa aaccctggcc
      301 cgggcctggg ccaaacccgg cacccccggc ctccagcacc gcatcggcgg gctggagaaa
      361 aacgatgtca ccggcgccgt gagctacgac cccgacaacc acgaaaagat gggccagatt
      421 cgggccgaaa aagtggcccg tgtggccgac gacatcgccc ccctggccgt ccgcggcgcg
      481 gaccggggcc gcctgctcgt cctgggctgg ggcagcacct acggccccat ttaccaggcc
      541 gtaagcgaag tgaccgaggc cggtcgcgcc gtctcccagg cccatctcac ccatttaaat
      601 cccttcccca aaaacaccgg ggaggtccta tccaaattcg aaaaggtcct cgtgccggaa
      661 ctcaacagcg gccagctgct catgctcctc cgccaccgct tcccccgggt ggatttcgtg
      721 ggctacaacc gcgtgcgcgg cgtgcccttt tacgtgtcgg aagttcgcga agccatcgag
      781 gcggccctat gaccgaagtt cccgttccct ccggcgccga aaagcgcccc acgttcacca
      841 aaaacgattt cgtttccggc gccgaggtcc gctggtgccc cgggtgcggc gactattcca
      901 tcctggggtc catgcagcag acgctggcga aattcgacat tccccgggaa aactacgtgt
      961 tcgtctcggg catcgggtgc tcgtcccgtt tcccctacta catgaacacc tacgggttcc
     1021 acacgatcca cggccgggcc ccgacgatcg ccaccgggct ccgctgcgtg aacccggacc
     1081 tttccatttg ggtcatcacc ggcgacggcg acggcttgtc catcggcggc aatcatctgc
     1141 tccacgccat gcgccgaaac ttgaatttca acatcctcct ggtcaacaac caaatttacg
     1201 gactgacgaa gggccaatat tccccgacgt cccccacggg caagatgacc aaatcctcgc
     1261 cctacgggtc catcgaccac cccatcaacc ccctgtgcat tgccatcgcc tcggaggcga
     1321 ccttcatcgc ccgcacgttg gattccgacc cgcgccacat gagcgccacc ctggaacggg
     1381 ccatgaagca caagggcatc gccttcgtcg aggtgtacca gaactgcatc attttcaacg
     1441 acaaggccca cgggcccgtg accggccggg aaacccggga cgaccggatg gtctatttgg
     1501 aacacggcaa accgctcctt ttcggcaaaa acaaatccaa agccatccga ttgaacggcc
     1561 tctatcccga aatggcggac tacgacccca ccaacacccg gggcctgctg atccacgacg
     1621 agacggaccc ccgtccgaac cacgcgtacc tgctcaccca attgacctac cccgatttcc
     1681 ccgtcccctt cggcgtcttc cgcgccgtgg agaaaccgac ctacgacgac ctgatgaacc
     1741 aacaaatccg ggacatcgtc gccaagaaag gcaagggcag cctcgaaaaa ttaatctaca
     1801 gcggcgacac ctggagggtc caatgagcgc gaacccctgc cccttttgcg gacacgagaa
     1861 tctccccggc gacgaccggt gcggccaatg cctccattcc ctgatgcagc gtcagattcc
     1921 gtcggcccgc cgggtgggcg acgagttccg ggacgccctg ctcaaggaac cggtttcgga
     1981 actcctgacg ggcgtcgacc tgttggtctg cagccccaag gactctctgg aaaaaatcct
     2041 caaaattttt caaaaagaga agaaggggtg cgtgctggtc tacgaaaaaa agaagatggt
     2101 cggcattctc tcgaaccggg acctgttgct gcgcgccgcg ggccccggcg atgatttgtc
     2161 caaactcacg gtcgaggacg tcatgacccg aaacccgagt tacgtccgtc ccgaggaccc
     2221 catcgctttt gccgtcaaca aaatgtccgt ggggggctac cgccacgtcc cggtcatccg
     2281 ggcggacggg acc
//
LOCUS       DAOQLS010000323         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30211_length_2289_cov_3.399284, whole genome shotgun sequence.
ACCESSION   DAOQLS010000323 DAOQLS010000000
VERSION     DAOQLS010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30211_length_2289_cov_3.399284"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..743
                     /locus_tag="PK362_06625"
     CDS             87..743
                     /locus_tag="PK362_06625"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNC74539.1"
                     /translation="MRKTPAAVLVSGGLDSLALLEWVRERHAPVHPLYVRFGLRWEKA
                     ELAALRRQGAARPAPGLRPVTVLDAPAAPLYGAHWSLAGGKVPGYRSADAAVFLPGRN
                     LLLLGLAGVYCARRKIPVVYFGALKGNPFADSRPPFLRAISRAVSLALGAPFRAVAPF
                     RRWSKSDLLARFPGAPYDRAFSCLNPRGLRPCGACNKCAEGDKARRFLSSTRKSNVIK
                     "
     gene            748..1371
                     /locus_tag="PK362_06630"
     CDS             748..1371
                     /locus_tag="PK362_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNC74540.1"
                     /translation="MSQRPLHVSVLGAGSAYGVFLVKWAYQLLRDPNSNRENLTFPPI
                     ASISYSNTDRRNTALVLEVLQNDLHQMPGLENVTTEEIGRHVRGYERWREMVEKEKPD
                     LVIVCSPTETHIPICRELITGFGVKNILCETPLTPVNDSDSLPGLEALVKEHGVTFGV
                     NQQYASLPAFLKDLRLNAAEPQSPTFGDVFQGAQAAEITFITHGPRP"
     gene            1465..1848
                     /locus_tag="PK362_06635"
     CDS             1465..1848
                     /locus_tag="PK362_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74541.1"
                     /translation="MKVEKVTREGDNIFLNLVEYELRFGSTPVKLVLGYRRKLKSLKL
                     VYAKGDREYDFHITGTTNPQTGEFTRGIEGKNYAFPFRYALRTDLVKYSFMRSLAGQP
                     LTPLSDAVQHQNIIRRIYEGSSKLK"
     gene            complement(1911..>2289)
                     /locus_tag="PK362_06640"
     CDS             complement(1911..>2289)
                     /locus_tag="PK362_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNC74542.1"
                     /translation="GWLDFDVLVCTPDAMKDLTKLAKILGPKGLMPNPKSGTVTFDLA
                     RTVKELKAGRIEFKMDDYAIIHSILGKASFPENKLAENARALLDAITAARPSAAKGTY
                     IRTVNLASTMGPSVSVVTESASK"
BASE COUNT          409 a          774 c          687 g          419 t
ORIGIN      
        1 ggcatggaaa aattttagca ctttgatggt ccccgggcgg ttccgggaaa ccccgggcgc
       61 gggacggatt tggtaaattc tcgtccatgc gaaaaacgcc cgcggcggtg ctggtgagcg
      121 gcggcctgga cagcctcgcc cttctggagt gggtccggga gcgccacgcg ccggtccatc
      181 ccctctacgt gcggttcggc cttcgttggg aaaaggccga gctcgcggcg ctgcggcggc
      241 agggagcggc ccgccccgcg ccgggccttc gccccgtgac ggtcctcgac gccccggccg
      301 cgcccttgta cggcgcccac tggagcctcg ccgggggaaa agtgccgggc tatcgctccg
      361 ccgacgccgc ggtctttctg cccggacgca atctgcttct cctcggtctg gcgggggtgt
      421 actgcgcccg tcgaaaaatc cccgtggtgt atttcggcgc cttgaagggc aaccccttcg
      481 ccgacagccg ccccccgttt ttgcgcgcga tctcccgggc ggtgtcgctg gccctgggcg
      541 ccccgtttcg agccgtggcc ccctttcgac gatggagcaa aagcgacctc ctcgcgcggt
      601 tccccggggc gccctacgac cgggcctttt cctgcctgaa cccccggggc cttcggccct
      661 gcggggcgtg caacaagtgc gccgaagggg acaaggcccg tcggttcctg tcctcgaccc
      721 gaaaatcaaa tgtgataaaa taatcccatg agccaacgtc cccttcacgt cagcgttctc
      781 ggcgccggtt cggcctacgg cgtgtttctg gtcaaatggg cctaccaatt gcttcgcgac
      841 ccgaattcca accgggagaa cttgactttc ccccccatcg cctccatcag ctattccaac
      901 acggatcggc gcaacaccgc cctggtgctg gaagtgctgc agaacgatct tcaccaaatg
      961 cccggcttgg aaaatgtgac gaccgaggaa atcggccgcc acgtgcgcgg ctacgaacgc
     1021 tggcgcgaaa tggtcgaaaa ggaaaagccc gacctggtca tcgtctgctc cccgaccgaa
     1081 acccacatcc ccatttgccg cgagctcatc acgggcttcg gcgtgaagaa catcctctgc
     1141 gaaacgcccc tgacgccggt gaacgattcc gacagcctgc cggggctcga agccctggtc
     1201 aaggagcacg gggtgacctt cggcgtgaac cagcagtacg cctccctgcc cgccttttta
     1261 aaggacctcc gcctgaacgc cgccgaaccc cagagcccga ccttcggcga cgtcttccag
     1321 ggagcccagg cggcggagat caccttcatc acccacggcc cccggcccta gcgccgcttc
     1381 ggcaacatcg gcgagctcat tatcctggaa gacctgggca ttcacgccct ggacttcctc
     1441 ccccccgccc tctggcggga acccgtgaag gtggaaaaag tcacccggga aggcgacaac
     1501 attttcctga acctggtgga atacgaactg cggttcggtt cgacgcccgt taagctcgtc
     1561 ctgggctacc ggcggaaact taaaagcctc aaattggtct acgccaaggg cgaccgggaa
     1621 tacgattttc acatcacggg caccaccaac ccccaaacgg gcgagttcac ccgcggcatc
     1681 gaaggcaaaa actacgcctt ccccttccgc tacgccctgc ggacggacct ggtcaagtat
     1741 tccttcatgc ggtccctggc cggccagccc ctgaccccgt tgtccgacgc ggtgcagcac
     1801 caaaacatca tccgccggat ctacgaaggg tcgtccaaac tcaaataacg attccataaa
     1861 aaaaggccca tcccccgcgg gggatgggcc ttttttgttt taaggggttt ttacttcgag
     1921 gccgactcgg tcaccaccga aacgctgggc cccatggtgg aggccaggtt cacggtgcgg
     1981 atgtacgtcc ctttggcggc cgaggggcgg gcggcggtga tggcgtccag gagggcccgg
     2041 gcgttctccg ccaacttgtt ttcggggaag gaggccttgc ccaggatcga gtggatgatg
     2101 gcgtagtcgt ccatcttgaa ctcgatgcgg ccggccttca attccttgac cgtgcgggcc
     2161 aggtcgaagg tcacggtgcc ggatttcggg ttgggcatca ggcccttggg cccgaggatc
     2221 ttggccagct tcgtgaggtc cttcatggcg tcgggcgtgc agaccagcac gtcgaaatcg
     2281 agccagcct
//
LOCUS       DAOQLS010000324         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30321_length_2285_cov_4.043049, whole genome shotgun sequence.
ACCESSION   DAOQLS010000324 DAOQLS010000000
VERSION     DAOQLS010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30321_length_2285_cov_4.043049"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK362_06645"
     CDS             <1..197
                     /locus_tag="PK362_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HNC74543.1"
                     /translation="GKYDNTWHFNHFKNPPALVEGSIMPSYAWFFDKEGNPNAKGLAI
                     VTYVQWLGTWPRVKGLDEEW"
     gene            191..445
                     /locus_tag="PK362_06650"
     CDS             191..445
                     /locus_tag="PK362_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74544.1"
                     /translation="MVTSKSKAVWVALIASGLFAAVVMMLGLLWQKKVTEYAKLAAGS
                     YILSTIGFLFLVGWTFFRGDLNDVEAPKHRVLKAETDDAD"
     gene            486..656
                     /gene="ccoS"
                     /locus_tag="PK362_06655"
     CDS             486..656
                     /gene="ccoS"
                     /locus_tag="PK362_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNC74545.1"
                     /translation="MDSRALIALFVSTLGLLVVGSVFMLVWSVASGQWDDSEKPKFKI
                     LEAESDGEPKNN"
     gene            1179..1250
                     /locus_tag="PK362_06660"
     tRNA            1179..1250
                     /locus_tag="PK362_06660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1211..1213,aa:Thr,seq:cgt)
     gene            1273..>2285
                     /locus_tag="PK362_06665"
     CDS             1273..>2285
                     /locus_tag="PK362_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74546.1"
                     /translation="MKSIETNNEASRPENSRPLTREGLGDLFSRLVAFSLPITYFLVT
                     VSFYLRTYDSAQIKITFTQIGCALVALFWGLQLIFQKRWPFSKADLPVVAPFLAVLGS
                     GCFSYAHSSFRNGSLDEFVRRVFYVLMALVVIAEFRGLDRQRRLFRWLLASFGITVLY
                     GFVQYFDGRLFPPGAGGVGLDPFIWRQAFGWRVFSSFGNPNFYGNFLVIVTPVILAFY
                     FKNGGRPFRPFLLMAVLVPVVVLTDKWLLNQFGGVTAATRPWVVIGLSAGLAVVGVLI
                     WWRSASAAASGLLIFFAATFVNLYATETKGAWVGFLGAMVATAILVGLFLVGRRARRV
                     TVGL"
BASE COUNT          411 a          613 c          667 g          594 t
ORIGIN      
        1 ggggaaaata cgacaacacc tggcacttca atcacttcaa gaatccgccc gccttggtgg
       61 aaggatcgat catgccctcc tacgcctggt ttttcgataa agaggggaat cccaacgcga
      121 aggggttggc catcgtcacg tacgtccaat ggctgggcac ctggccccgg gtgaaagggt
      181 tggacgaaga atggtgacct ccaaatccaa ggccgtttgg gtcgccctga tcgccagcgg
      241 attgtttgcg gcggtcgtga tgatgttggg cctcctttgg caaaaaaaag tcacggaata
      301 cgctaaattg gccgcgggca gttacatcct ttcaacaatc gggtttcttt tcctcgtggg
      361 atggactttt ttccgggggg atctcaacga cgtggaggcc cccaagcacc gggtcttgaa
      421 agccgaaacc gatgacgcgg actaaatggc tcagcggctc aagctgtttt tgttttttct
      481 gcggagtgga cagccgggcg ttgattgccc tgttcgtttc caccctcggg ctgttggttg
      541 tgggatcggt ttttatgctg gtttggagcg ttgccagcgg ccagtgggac gatagcgaga
      601 agccgaagtt taaaattctg gaggccgaat ccgatggcga acccaaaaac aactgaagac
      661 cttggctcga ttcccccggc ggaacacggg aagatgcctc gttggctttt tctgatctgg
      721 gtggcccttg tggtgtggag cctatattat gtcgttcggt atgccttgcc ggattacaag
      781 gattggacga agcgccctcc cctgacgacc gctgaacgcc cgtaattttg aaataaaaac
      841 ggccgcgtcg tcctgttcgg cgacgcggcc gtttgttgtt ttcgaggttc ttttaagata
      901 acatagcgcc ttcccgcgcc aaattcaacg tcgttgtctt ttcgcgaggg aaggccagac
      961 cgtcctccca atggggaagt ttacgtcgaa gggcctcgat ctcgttaatt ttaaacaggt
     1021 cgcccttgcg cacagagcaa ggaccttgct aatcggcacg gttcccggat tgtcggaagg
     1081 ggacagtccg gcggccgcgg ggcggccgaa acccctggaa gccgcccttg ctcagagcgc
     1141 aaggacaggc aggcggccgc ggagcggccg aaacccctgc cgaggtagct caacggtaga
     1201 gcaatcgctt cgtaagcgat aggttgtggg ttcaaatccc atcctcggct tatttttaac
     1261 gatggttctt tcatgaaaag cattgaaacc aacaacgagg cgtcccgtcc ggaaaacagc
     1321 cgtcccttga ctcgcgaggg tttgggggat ttgttttccc gactcgtggc cttttccctg
     1381 cccattacct atttcttggt aacggtttct ttttacctcc gcacgtacga ttccgctcaa
     1441 atcaaaatca ccttcactca aattggttgc gccctcgtcg cgttgttctg ggggctgcaa
     1501 ttgatattcc aaaaacgatg gcccttttcc aaagcggacc tgcccgtggt cgcgccgttc
     1561 ctggccgttt taggttcggg ttgcttctcc tacgcccact cctcttttcg aaatgggagt
     1621 ttggacgagt tcgtccggcg ggtgttttat gtcctgatgg ccttggtggt cattgccgaa
     1681 ttccgcggct tggaccggca gcgccgactt tttcgatggc tattggcttc cttcggaatc
     1741 accgtgctgt atgggtttgt gcagtatttc gacggtcgtc ttttccctcc gggggcgggg
     1801 ggcgtcggtt tggatccctt tatttggcgc caagctttcg gttggcgcgt tttttccagc
     1861 tttggaaacc cgaattttta cggaaatttc ctcgttatcg tcaccccggt aatcttggct
     1921 ttttacttta aaaacggggg acgccctttc cgacccttcc tcctcatggc ggtgcttgtg
     1981 ccggtcgtgg tgttgaccga caagtggctg ttgaaccaat tcggcggagt aaccgccgcc
     2041 acccgcccct gggtggtcat cggattatcg gccgggttgg ccgtggtcgg cgtcttgatt
     2101 tggtggcgca gcgcctcggc ggccgcctcc ggtcttttaa tattctttgc ggcgaccttt
     2161 gtgaacctct acgccacgga aaccaaaggc gcttgggtcg ggtttttggg ggccatggtg
     2221 gccacggcca tcttggtggg attgtttttg gtgggccgac gcgcccgacg cgtcacggtc
     2281 ggcct
//
LOCUS       DAOQLS010000325         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30348_length_2284_cov_3.505608, whole genome shotgun sequence.
ACCESSION   DAOQLS010000325 DAOQLS010000000
VERSION     DAOQLS010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30348_length_2284_cov_3.505608"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PK362_06670"
     CDS             complement(<1..904)
                     /locus_tag="PK362_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNC74547.1"
                     /translation="MNSRRRSWAVPALLALLAGAFAFSLALGAVPLGAWSDLRPGGTS
                     WDILFRLRLPRVLLGAAVGALLSAAGCVLQGVLRNPLADPYLLGLSGGAALGSAVGFL
                     LGIAQPAPLAVIGALVSLGLVLLLSRGAGAPSATLMVLSGAALHAFTSSVLTFILSQA
                     RRDQGANILFWLLGSLDSPPYGRLIPLLLLSAAALAGLCWASPALNLLALGRDTARAL
                     GLSVDKALWAMVLLSSLATGLAVTFNGVIPFVGLMVPHMARLLVGADHRWSVPASAVL
                     GAALVLLADALGRWALAPQEIPVGV"
     gene            complement(901..1716)
                     /locus_tag="PK362_06675"
     CDS             complement(901..1716)
                     /locus_tag="PK362_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNC74548.1"
                     /translation="MRFIRAIAVFLVAAPLWAGARERRLVSLLPSHSEIVTALGTRRS
                     LVAVSDSEVAGDFPGLPRAGALEPRWEVLMALRPDLILADSSHERFAADFQRHRLPVL
                     FLPATHAKSIEDVFDLIRKVGDAIDRAPQAAALVSNLKERLAPLDARPRPAHRPRVYF
                     EIWPRPLQSVGAASFQGQLLTRAGFDNIVPDTTNEMPLISSEWVVKNRPEVVFHTGVE
                     GAGDLGSRPGWKDLPAVRSGRVIRVDADLFSRAGPRVVEALAYLMDVASAVQP"
     gene            2096..2245
                     /locus_tag="PK362_06680"
     CDS             2096..2245
                     /locus_tag="PK362_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74549.1"
                     /translation="MDRFYDIFVHIYALISQNFRWDHSLSEPDPVLAPADSPRYRKRK
                     TLRRG"
BASE COUNT          482 a          771 c          748 g          283 t
ORIGIN      
        1 caacgccgac cgggatttcc tgaggcgcca gagcccaacg acccagggcg tcggccagga
       61 ggaccagggc cgcccccaga acggccgagg ccggcacgct ccaacggtgg tcggcgccca
      121 cgagcaaccg ggccatgtgg ggaaccatca aacccacgaa gggaatgacc ccgttgaagg
      181 tgacggccaa gcccgtcgcc agggaggaca gcaggaccat ggcccagagg gccttgtcca
      241 cggaaagtcc cagcgcccgg gccgtgtccc ggcccagggc caggaggttc agcgcgggcg
      301 acgcccaaca gaggcccgcc agggccgccg cggacaataa gagcaaaggg atcaaccgcc
      361 cgtagggcgg cgagtccaga ctgcccagca gccaaaagag aatgttggcg ccctggtccc
      421 gccgcgcctg ggagaggatg aaggtgagca ccgacgaggt gaaggcgtgc agggccgccc
      481 ccgacaagac catcaacgtg gcgctcggcg cgcccgcgcc ccgggacaag agaaggacca
      541 gccccaggga aaccagggcc ccgatcacgg ccaagggcgc gggctgggcg atccccagaa
      601 gaaatccgac ggccgacccc agggccgctc cgccggaaag ccccagcaaa taggggtccg
      661 ccaggggatt tcgaagaacg ccctgaagca cgcaccccgc ggccgaaagc agggcgccca
      721 ccgcggcccc cagcagaacc cgggggagcc gcaggcgaaa caaaatatcc caggacgttc
      781 cgcccggacg aaggtcgctc caggcgccca ggggcaccgc gcccagggcc agggaaaagg
      841 cgaaggcgcc cgccagcagg gccaggaggg cgggcaccgc ccaggaccgt cggcgggaat
      901 tcacggctgg accgccgacg ccacgtccat caaataggcc agggcctcca ccacccgcgg
      961 ccccgcccgg gaaaacaaat ccgcgtccac ccgaatcacc cgcccggagc gcaccgcggg
     1021 gaggtctttc caccccggac gggaccccaa gtccccggcg ccctccaccc cggtgtgaaa
     1081 aacgacctcc ggccggttct tcaccaccca ctcggaggag atcaggggca tttcgttggt
     1141 ggtgtcgggc acgatgttgt cgaaaccggc gcgcgtcaac aattgtccct gaaaactggc
     1201 ggcccccacg gattggaggg ggcggggcca aatttcgaaa tacacccggg ggcgatgggc
     1261 gggccggggc cgggcgtcca aaggggccag acgctccttc aggttggaaa ccaaggccgc
     1321 ggcttgcgga gcgcgatcga tggcgtcgcc caccttccgg atcaaatcaa aaacgtcctc
     1381 gatggatttg gcgtgggtgg cgggtaggaa gaggacgggg aggcgatggc gttgaaaatc
     1441 cgccgcaaac cgttcgtggg aactgtccgc caggatcaaa tcggggcgaa gggccatcaa
     1501 gacttcccag cggggctcca gagccccggc ccgggggagg cccgggaaat ccccggcgac
     1561 ttccgaatcc gacaccgcga ccaaagacct ccgcgtcccc aacgccgtca cgatttcgga
     1621 atgggaggga aggagggaaa ccaggcgtcg ttcacgggcc cccgcccaaa gaggcgcggc
     1681 cacaaggaaa acggcgatcg ctcggataaa acgcatgtcg gacccaacgc cgggcgggaa
     1741 aagaggggac aaaaaaaccc aacagggcgg gagtctgttg ggggtcgatc gtccttcccg
     1801 cgaaggcgcc tttcggcgaa gtatggctcg cgcgccgtag accggacttg tccccgaccg
     1861 tttcgtcggg gcttcaccgt tgcggggcag tgcctgattc gaccgatgcg gtccgacagg
     1921 gcttccccgg cgtgcgagaa tgggttcagt gtatcaaaaa cggcccggtt gtcaactttc
     1981 cgacgatcca agatcccctt gatttatttc ccaaccgggg gtaggctggt tcggttcgct
     2041 tcccccttca acgagaccga cacaaggaca atccatccgc cgggaggact ttgccgtgga
     2101 tcgtttctac gacatcttcg ttcatatata cgccttgatc agtcagaatt tccgttggga
     2161 ccattccctt tccgagcccg accccgttct ggccccggcg gattcccccc gctaccgaaa
     2221 acgaaaaaca ctccgccgcg gctgacggcc cgaccccgcc ctcgctgacc cgggcgttga
     2281 atcg
//
LOCUS       DAOQLS010000326         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30428_length_2281_cov_3.543127, whole genome shotgun sequence.
ACCESSION   DAOQLS010000326 DAOQLS010000000
VERSION     DAOQLS010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30428_length_2281_cov_3.543127"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="PK362_06685"
     CDS             complement(<1..113)
                     /locus_tag="PK362_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC74550.1"
                     /translation="MTEKRPPAPDDTALRVALWRALHVEADPPPHIFEDVIG"
     gene            complement(106..639)
                     /locus_tag="PK362_06690"
     CDS             complement(106..639)
                     /locus_tag="PK362_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74551.1"
                     /translation="MALVLLSLPTPALPEIARLVLSDDGTSLHFETTDGKSVAVDPLR
                     DQVGFTQVRLSEDRKRLGWVALFPNCCTSYPVPLKLAVSSENGSVVVFPASQAIFKWN
                     FAKGSKAVVYRQEALHGPAVATYSMGRLKDGKILKTFVDAPAEAPGANPAGPPSWVAG
                     ASDEALLSENQTREDHD"
     gene            complement(671..1180)
                     /locus_tag="PK362_06695"
     CDS             complement(671..1180)
                     /locus_tag="PK362_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74552.1"
                     /translation="MSAPLSIGHFTLRERKVQVLRSWGDPDFFRAQMAAGNVRPPKKD
                     PVVRRKVASWIFRGHAVEVSFDPDSENETEAVERINVFLNGLESGLGTLKGAIAGWLL
                     DAYNNEWAAGRQRLTRDQFEEALGPLKDIEYSKTRTTLYWDDNGLFDRHVIEVRMKNE
                     KVTEVLIAG"
     gene            complement(1218..1580)
                     /locus_tag="PK362_06700"
     CDS             complement(1218..1580)
                     /locus_tag="PK362_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC74553.1"
                     /translation="MDVKSLFENDDTVVAFRAGSSIFEEGKTGTVMYVVLDGQVEIRK
                     GAKAIETLGPGSILGEMSLIAAPKRSATAVAATDCRLASVDERRFLALVREYPFFALH
                     VMRALAERLRRMNDLLTP"
     gene            complement(1667..1987)
                     /locus_tag="PK362_06705"
     CDS             complement(1667..1987)
                     /locus_tag="PK362_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74554.1"
                     /translation="MKLFLNVPLSLEEVASVVGKIALPDYPPQRREGLNLGGGDYFIF
                     TKESSTVLLVCNDDRHAEVYVESQKAFPYYLYVLRGGDGVLEKAHEALSKRGIECRLK
                     AESP"
     gene            complement(2036..>2281)
                     /locus_tag="PK362_06710"
     CDS             complement(2036..>2281)
                     /locus_tag="PK362_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74555.1"
                     /translation="WLAFTLSFLLPGAGLAYLGKWKWAAVNLGFVLSIGVLSGMFLSE
                     DLLNHYARFIAIGLGAGSAGLAQSLADQMNKKMRNTP"
BASE COUNT          480 a          623 c          701 g          477 t
ORIGIN      
        1 ccgatgacgt cttcaaaaat atggggcggg gggtcggctt ccacgtggag ggcccgccac
       61 agagcgaccc gaagagcggt gtcgtcgggg gcgggcggtc gtttttcagt catggtcttc
      121 ccgtgtttga ttttccgaaa ggagggcttc atcggaagca ccggccaccc aggagggggg
      181 accggccggg tttgcgccgg gagcctccgc tggggcgtcc acgaaagttt ttagaatttt
      241 cccatctttt aggcgcccca ttgagtaggt ggccacggcg gggccgtgca gggcttcttg
      301 gcggtagacc accgccttgg accccttggc aaaattccat ttgaaaatgg cctgggaagc
      361 cggaaacacg acaaccgatc cgttttccga ggaaacagcg agtttgagcg gcacgggata
      421 agaggtgcag caattgggga acaacgccac ccatcccagg cgtttgcggt cctcggacag
      481 ccgtacctgg gtgaagccga cttggtcccg caagggatcg acggcgacgc ttttgccgtc
      541 ggtcgtttcg aaatggaggg aggtcccatc gtcgctcagg acgagccggg ctatttccgg
      601 caaggcgggg gtgggcagac ttaggaggac aagggccaat agtcgtttta tggacgtatc
      661 ggatctccgc tcaacccgcg atcaagactt cggtgacctt ctcgtttttc atccgaactt
      721 caatgacgtg tcggtcgaac aaaccgttgt cgtcccaata gagcgtcgtg cgggtttttg
      781 aatattcgat gtctttcaaa ggacccaacg cctcttcgaa ttggtcgcgc gtcagtcgtt
      841 gccggcccgc ggcccattcg ttgttgtagg cgtcgagcag ccaacccgcg atggctcctt
      901 ttaacgtccc gaggccggat tccagcccgt tcaagaaaac attgatgcgc tcaacggcct
      961 ccgtttcgtt ctcggaatcc ggatcgaagg acacttccac ggcgtggccg cgaaagatcc
     1021 aggaggcgac ttttcggcga acgacgggat ccttcttggg gggacggacg ttgcccgcgg
     1081 ccatttgtgc ccggaaaaaa tcgggatctc cccaggaccg gaggacctgg acttttcttt
     1141 cccgcagggt gaaatggccg atggacaggg gggcgctcat ggtttcttca ataacgcatt
     1201 tgtcgtcggc tcaaaaatta cggggtgaga aggtcgttca tccggcgcag gcgttcggcc
     1261 agagcgcgca tgacgtgcag ggcgaaaaag gggtattccc gaaccagggc gagaaaccgt
     1321 cgttcgtcca cggaggccaa tcggcagtcg gtggcggcca cggcggtggc gcttcgtttg
     1381 ggcgcggcga tcagggacat ctcgcccaga atggacccgg gcccgagggt ttcgatggcc
     1441 ttggcgccct tgcggatttc cacctggccg tccaggacga catacatcac cgtcccggtc
     1501 ttgccttcct cgaaaataga ggatccggcc cgaaaggcca cgacggtgtc gtcgttttca
     1561 aaaaggcttt ttacgtccat gggacccccc ccaagtgaaa atggtcctta aaggatagct
     1621 caaaagaaag gattacgccc cggccgccag cgtccggccc aaagggctaa ggggactcgg
     1681 ccttcaggcg acattcaatg cccctcttcg atagggcttc gtgcgctttt tcaagaacgc
     1741 cgtctccgcc ccgaagaaca tagaggtaat acggaaacgc cttttgggat tcgacataaa
     1801 cttcggcgtg acggtcgtcg ttgcaaacca gaaggaccgt cgagctctcc ttggtgaaaa
     1861 taaaataatc gccgcctccc aaattcaggc cttcccgccg ttgaggcggg taatccggaa
     1921 gggcgatttt gccgaccacc gaagcgactt cttccaggga aagagggacg ttgaggaaaa
     1981 gtttcatggt cggcgatttt gaattgggat ccaggggtgc gcgtttcggc cgactttatg
     2041 gggtgtttct catttttttg ttcatttgat cggccaggga ttgagctaaa ccggcggagc
     2101 cggcgcccaa tccgatggcg atgaatcggg cgtagtgatt taaaaggtct tcggataaaa
     2161 acattcccga caaaacgccg atggagagaa cgaagcccag attgaccgcg gcccacttcc
     2221 atttgccgag gtaggccaat ccggctcccg gcagaaggaa actcaaggtg aatgcaagcc
     2281 a
//
LOCUS       DAOQLS010000327         2274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30597_length_2274_cov_4.398378, whole genome shotgun sequence.
ACCESSION   DAOQLS010000327 DAOQLS010000000
VERSION     DAOQLS010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30597_length_2274_cov_4.398378"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="PK362_06715"
     CDS             complement(<1..2260)
                     /locus_tag="PK362_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNC74556.1"
                     /translation="MFVGKGSRRGATMIELVVAAVLVGVGITVSIKVFQGIAKSVFLS
                     QTKQVATTLAREKLEALQVMPYYRLRAAGAPTYYQPGGSGAPYFYYDPYFTPGPERIQ
                     VGNVTYYRGAYVERVYRDPATGNLAALAAATPYQTEAGMKRIKSSVMWKDGLTWRVAD
                     VTGLKENPNRTRGEVKFVGVITDTNGVPLSGALGIRVDTIETLGQMTNASPNAAGYYE
                     FSIRPGTYTIRARDISTNWNYYTAKQAAYVGYEMGVTTVNFQMSRFTYGRVDAAFWWN
                     SSPVISRVVGSSASFSNPAFNQEWVEVFNPTTWTWDATKLNLHYYMTHNSSEPAANAL
                     PNTTADYLQHKIEVNYINTAIPPGGYFLFANAPTIDLQEGSVNADAVWETVNPSSNAW
                     WRVDRPGGAVGRFDPNGLLNAGQPNPDILTTAETANYLPSQSTWNFVLGVPAVQYNGG
                     GGYVRLTYSQNSSTDYIYLDGVGWRGGLDAGCVGGGRDPVAAEEGYRLVDKAGGIEDC
                     AGLQAGEQYLRYASTMAWMESRDVGPAYDSNCNVRDWSKYTAAAPGYFARFLPRNSVA
                     APVPVVSGRDDWTRASNRLAGGNEAGFVNAFVNDGLTPTLDGLKPTYYSGWTPSSAGY
                     CTTCPRSNMYNYTNSDIPWWGRFEFLRVSTGLWTLGMYARRITGGSGTGGTPGQTPYD
                     YHTSSQITVNVTPGSYTFWQGVVGETTDKVFLFGSARDVYDVPIEDLRVEYKVSNGTP
                     AGAVLDFTRTRVG"
BASE COUNT          414 a          696 c          719 g          445 t
ORIGIN      
        1 cccccacccg cgtgcgcgtg aagtcgagta cggccccggc gggcgtcccg ttgctcacct
       61 tgtattcgac cctcaaatcc tcgatgggca cgtcgtacac gtcccgggcg gaaccgaaca
      121 aaaacacttt gtccgttgtt tcgccaacga ccccctgcca aaaggtgtaa cttccggggg
      181 tgacgttgac ggtgatttgc gaagacgtgt ggtaatcgta gggcgtttgt cccggggtgc
      241 ccccggtgcc gctccccccc gtgatccggc gggcgtacat gcccagggtc caaagaccgg
      301 tggaaacccg cagaaattca aaacggcccc accaggggat gtcggaattc gtgtagttgt
      361 acatgttgga ccgggggcaa gtggtgcaat agccggcgct gctgggggtc cagccgctgt
      421 aataggtcgg ttttaacccg tccagggtcg gggtcaggcc gtcgttaaca aaagcgttga
      481 cgaacccggc ttcgtttccg cccgccaggc gattgctggc ccgggtccag tcgtcccgcc
      541 cggacacgac cgggacgggg gcggcaacgc tgttccgggg caggaaccgg gcgaaatagc
      601 ccggcgccgc cgccgtgtat ttggaccaat cccggacgtt gcagtttgaa tcgtaggcgg
      661 gtccgacgtc ccgggattcc atccaggcca tggtgctggc gtagcgaagg tattgctcgc
      721 cggcctggag accggcgcaa tcctcaatgc cgccggcttt gtcgaccaag cggtatcctt
      781 cctcggcggc caccggatcc cggccgccgc cgacgcatcc cgcgtccaga ccgccgcgcc
      841 accccacccc atccagatag atatagtccg tggaggaatt ttgactgtag gtcaagcgaa
      901 cgtacccccc gcccccgttg tattgaaccg ccgggacgcc caggacaaag ttccaagtcg
      961 actggctggg aagatagttg gccgtttccg ccgttgtgag gatgtcggga ttcggctgac
     1021 ccgcgttcag caacccgttc gggtcaaacc gcccgaccgc ccccccgggg cgatccacgc
     1081 gccaccaggc gttggaagaa gggttcacgg tttcccacac ggcgtcggcg ttgaccgacc
     1141 cctcctgcaa atcgatcgtc ggcgcgttgg caaagaggaa ataaccgccg ggcggaatgg
     1201 cggtgttgat gtagttcacc tcgatcttgt gctgcagata gtcggccgtg gtgttcggca
     1261 gggcgtttgc cgccggctcg gaactgttgt gggtcatgta ataatgcagg ttcaacttcg
     1321 tggcgtccca ggtccaggtc gtggggttga acacttccac ccattcctga ttaaacgccg
     1381 ggttcgaaaa actggccgaa ctgccgacca cgcggctgat gaccgggctc gaattccacc
     1441 agaaggcggc gtcgacccgg ccataggtaa accgcgacat ttgaaagttg accgtggtga
     1501 cgcccatttc gtacccgacg taagcggctt gtttggccgt gtagtagttc cagttggtgc
     1561 tgatgtcccg ggctcgaatg gtgtaggtgc cgggccgaat ggaaaattca tagtaccccg
     1621 ccgcgttcgg cgaagcgttc gtcatttgac ccagggtttc aatggtgtcc acacggatcc
     1681 ccagggcccc gctcaggggg acgccgttgg tgtccgtgat gacgccgaca aatttgacct
     1741 cgccgcgggt gcggttcgga ttttccttca gccccgtcac atcggccacc cgccaggtga
     1801 gtccgtcctt ccacatgacc gacgacttga tgcgtttcat gccggcttcc gtctgatagg
     1861 gcgtcgcggc ggccagggcg gccaaattgc ccgtggcggg gtcgcggtac acccgctcca
     1921 cgtaggcgcc ccggtagtag gtcacgttac caacctgaat gcgctcggga ccgggggtga
     1981 aatagggatc gtaatagaaa tacggggcgc cgctgccccc gggttggtag taggtgggag
     2041 ccccggcggc tcgaagccgg taatagggca tgacttgaag agcttccaac ttctcccggg
     2101 cgagggtggt ggcaacttgc ttggtttgac tgaggaaaac ggatttggcg atgccctgaa
     2161 acactttgat ggaaacggta atcccgacgc cgaccagaac ggcggccacg accaattcga
     2221 tcatggtcgc cccgcggcgg gatcccttcc cgacgaacat ggctcctcct tgtt
//
LOCUS       DAOQLS010000328         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30684_length_2271_cov_3.829422, whole genome shotgun sequence.
ACCESSION   DAOQLS010000328 DAOQLS010000000
VERSION     DAOQLS010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30684_length_2271_cov_3.829422"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK362_06720"
     CDS             complement(<1..276)
                     /locus_tag="PK362_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNC74557.1"
                     /translation="MTGAPLQRTPFYAHHVAAGGKLVPFAGWEMPVQFSTVMAEHKAV
                     RERAGVFDISHMGQVWVAGPAARAFLQNLVTNDVGALPPGRGVYALLC"
     gene            complement(273..650)
                     /locus_tag="PK362_06725"
     CDS             complement(273..650)
                     /locus_tag="PK362_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC74558.1"
                     /translation="MKQHSPGFLKLVEDAKSRVREIGVAEVRSRLARREPFRFIDVRE
                     DREWAQGHAAGAEHMARGVLERDVEKAVPDFNTPLVLYCGGGFRSALAADSLQRMGYQ
                     NVLSLAGGWSAWVDAGGPVEGAR"
     gene            complement(647..1507)
                     /gene="lipA"
                     /locus_tag="PK362_06730"
     CDS             complement(647..1507)
                     /gene="lipA"
                     /locus_tag="PK362_06730"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNC74559.1"
                     /translation="MTVPMLFRPRLPPHLKRTVPSGEIYERLQAALRASELPTVCEEA
                     RCPNRTECWARGTLTFQILGAVCTRRCAFCAETTGRPGAVDSREPAKLVEAARRLALR
                     HVVITSPARDDLDDQGAGHFVACVEALRRDLPAVTVEVLVPDFQGRVDLLERVFRSRP
                     DVFNHNLETVRRLTPRVRARATYDRTLSVLAAAAGAGLHVKSGLMVGLGETSKELDEA
                     FRDLSGAGVRSLTVGQYLPPSSDHHPVDRFYTLPEFEELRRRAAPLFEKAMVGPLVRS
                     SYHADDMVNS"
     gene            complement(1504..2214)
                     /locus_tag="PK362_06735"
     CDS             complement(1504..2214)
                     /locus_tag="PK362_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC74560.1"
                     /translation="MRVLQTAFASRGIDVRTGTTVRSAQRSGVGWVLTLEDGGTVSVE
                     ELLVCVGRKPSVAGLGLEAAGVRVERDRPVVDAFLKTSHPAVYAVGDLNGLSLLAHAA
                     AAQAEIAVDHAFGESRPYRNERVPRCLYTWPEVASVGDWTHTAQARGVETKAQRFFFK
                     GSPKALAANEGDGFLQIVSEKAGGRILGAQIVGPHATEIIHVFAVALAAEMTVPRLRD
                     VIYAHPTLSEGIREALSR"
BASE COUNT          407 a          790 c          736 g          338 t
ORIGIN      
        1 gcaaagcaag gcgtacacgc cccggcccgg aggcaaggcg ccgacgtcgt tggtgaccaa
       61 attttgaagg aacgcccggg ccgccggtcc ggcgacccag acctggccca tgtgggagat
      121 gtcaaaaacg ccggcccgtt cccgcacggc cttgtgctcg gccatgacgg tggaaaattg
      181 gacgggcatt tcccagccgg cgaaggggac cagtttgccc cccgccgcca cgtggtgggc
      241 gtaaaaaggg gtgcgttgga gcggggcgcc ggtcatcggg ccccctccac cgggccgccg
      301 gcatccaccc aggcggacca gccgcccgcc agcgaaagga cgttttggta gcccattctt
      361 tgaaggctgt cggccgccag ggccgaacgg aacccgccgc cgcaataaag caccaggggg
      421 gtattgaaat ccggaacggc tttttccacg tcgcgctcca gcacgccccg ggccatgtgc
      481 tcggccccgg ccgcgtggcc ctgggcccat tcgcggtctt cccgaacgtc gatgaaacgg
      541 aacggctccc gccgggccaa ccgcgaccgc acctcggcca cgccgatttc ccgaacgcgg
      601 gatttggcgt cctcgaccag ctttaaaaat ccgggggaat gctgtttcat gaattcacca
      661 tgtcgtccgc gtggtaggaa ctgcgcacca ggggaccgac catcgctttc tcaaacaacg
      721 gcgccgcccg ccgccgcaat tcttcaaact cggggagcgt ataaaaccgg tccacggggt
      781 ggtggtcgga actgggcgga agatactgcc ccaccgtgag cgaccgaacg ccggcgccgc
      841 tcaaatcccg gaacgcttcg tccagttcct tggaggtttc ccccagaccg accatcaatc
      901 cggatttcac gtgaagaccc gcgcccgcgg cggcggccag caccgacaac gtgcggtcgt
      961 aggtcgcccg ggcgcgcacc cggggggtga gccgccggac ggtttccaaa ttgtggttga
     1021 agacgtccgg gcgggatcgg aacacccgtt ctagcaaatc gacgcgcccc tggaaatcgg
     1081 gcaccaggac ctcgaccgtg acggcgggca ggtcccggcg gagggcctcc acgcaggcga
     1141 cgaagtggcc ggcgccctga tcgtccaaat cgtcccgggc cggggaggtg atcacgacgt
     1201 ggcggagggc caatcgtcgg gcggcttcca ccagcttggc cggttcccgg gaatccacgg
     1261 ccccgggccg tcccgtggtc tccgcgcaaa aggcgcaccg gcgggtgcaa acggccccca
     1321 aaatttgaaa cgtcagcgtg ccccgggccc agcattccgt tcggttgggg caacgggcct
     1381 cttcgcaaac cgtcggcagt tcgctcgccc gcaaagccgc ctgcagtcgt tcgtaaattt
     1441 cgcccgaggg aacggtccgc ttcaaatgcg gcggcaaacg cgggcggaaa agcatgggga
     1501 ccgtcatcgg gacagagcct cccggatccc ctccgacaag gtggggtggg cgtaaatcac
     1561 gtcccgcaat cggggcacgg tcatctcggc ggccagggcc acggcgaaca cgtggatgat
     1621 ttccgtcgcg tggggaccga cgatctgggc gcccaggatc ctcccgccgg ccttttccga
     1681 aacaatctgg aggaacccgt cgccctcgtt ggcggccagg gccttgggcg aacctttgaa
     1741 gaaaaatcgc tgggccttgg tttccacccc ccgggcctgg gccgtgtggg tccagtcccc
     1801 cacgctggcg acctccggcc aggtgtacag gcaccggggc acccgttcgt tgcggtaggg
     1861 ccgggattcg ccgaaggcgt ggtccacggc gatttccgcc tgggccgcgg ccgcgtgggc
     1921 caggagcgac aatccgttca ggtcgcccac cgcgtagacg gcggggtggg aggtttttaa
     1981 aaaagcgtcg accaccggcc ggtctctttc cacccgcacg ccggccgcct cgaggcccaa
     2041 tcccgcgacg gagggtttcc ggcccacgca aaccaacagt tcttcgacgg acaccgtccc
     2101 gccgtcctcc aacgtcagaa cccaacccac accgctccgc tgggcggagc gaaccgtcgt
     2161 tcccgtccgg acgtcgatcc cccgggaagc gaaggccgtt tgaaggacgc gcaccaccgc
     2221 cgtcctccaa cgtcagaacc caacccacac cgctccgctg ggcggagcga a
//
LOCUS       DAOQLS010000329         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_30822_length_2265_cov_4.359276, whole genome shotgun sequence.
ACCESSION   DAOQLS010000329 DAOQLS010000000
VERSION     DAOQLS010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30822_length_2265_cov_4.359276"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PK362_06740"
     CDS             <1..613
                     /locus_tag="PK362_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNC74561.1"
                     /translation="PPLRVEPVIGLLPGSRPSEVRRHLPLFLAAADRLVKHRPGLRFV
                     LFAAPNLPNDFYDLLLGGDPKRPYRVELVRDEKYQWRAGLDLALTCSGTATLENALLG
                     LPMIVVYKTSWITYALARALVRVKHIAMPNVLAGREIVPEKIQSAATPEAIAGEVENL
                     LNDASLRRRVRRDLLGLRVALGGAGAARRAAEAIKAEAQGASA"
     gene            610..>2265
                     /locus_tag="PK362_06745"
     CDS             610..>2265
                     /locus_tag="PK362_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC74562.1"
                     /translation="MSGINDLISKGPLLDERTAKDPSASLVARRLLPYFKPHGPRLAL
                     ALAAMAGVAGLTAGAMWILKQVIDRALLDRDLAMLADTVLLVLLLYFAKAALSYVHDY
                     VTAYIGQAIVKQLRDQAYNNIHNLSLDFYTGTDSARVIARLTNDAQLVQNALTKIPIM
                     IVRDGLTVVALTGFLFYLHWRFALIAFSLLPVSGYLIARFGKSLRKTSRQGQAKMSDL
                     YTLIQETIAGAPVVKAFQREDYEKARFEAENRAYFDIYMRNARVESLSSPVMEFLGAI
                     GMGVILWFGGKDVVHHVWTAGAFFAFIGSALSLYQPVKNFSRSNATLQLAFAGAERMF
                     ELVDARPTVVEKPNPTDLGPFSDRIDFDRVSFAYRSDQPVLKDIQLTVRRGEIVALVG
                     PSGSGKTTLSALLLRFYDPRSGALRIDGVDLREASFKSLRGQIGLVTQETVLFNDTIR
                     HNIAYARADATEAEIIAAAQAANAWEFIRDKPRGLDTLIGERGVLLSGGQKQRLALAR
                     AILTDPRILVLDDATSAVDAATEEAIHATLRTLMQGRTTLIIAH"
BASE COUNT          377 a          792 c          665 g          431 t
ORIGIN      
        1 cccgccgctt cgggtggaac ccgtgatcgg cctcctgccc ggaagccgcc cctcggaagt
       61 ccggcgccac ctgccccttt ttctggccgc ggcggaccgg ctggtgaagc accggccggg
      121 ccttcggttc gtcctcttcg ccgccccgaa cctgcccaac gatttttacg acctccttct
      181 gggcggcgac cccaagcgcc cttaccgggt ggagctcgtg cgggacgaaa aatatcaatg
      241 gcgcgccggg ctcgatctgg ccctcacctg ttccggcacg gccaccctgg agaacgcctt
      301 gctgggcctt cccatgatcg tcgtttacaa gacctcctgg atcacctacg ccctggcccg
      361 ggcgttggtc cgtgtgaaac acatcgccat gccgaacgtc ttggccggcc gggagatcgt
      421 tccggaaaaa atccaatcgg ccgccacccc ggaggccatc gccggagagg tggaaaattt
      481 attaaacgac gcgtcgcttc gtcgacgggt gcgccgggat ctcttgggcc ttcgggtcgc
      541 cctgggcggc gcgggcgccg cccggcgggc cgcggaggcc atcaaagccg aggcccaggg
      601 agcttccgcg tgagcggcat caacgatttg atatccaaag ggcccctcct ggacgaacgg
      661 acggccaagg atccctcggc ctcccttgtg gcccgacgcc ttttgcccta tttcaaaccc
      721 cacggccccc ggttggccct ggccctggcg gccatggccg gcgtggcggg cctcaccgcc
      781 ggcgccatgt ggatcctcaa acaagtgatc gaccgggccc tcctggaccg ggatttggcc
      841 atgctggccg acaccgtgct cctggtcctc ctcctctatt tcgccaaagc ggcgctctcc
      901 tacgtgcacg attacgtgac cgcttacatc ggccaggcca tcgtcaaaca acttcgcgac
      961 caggcctaca acaacatcca caacctgtcc ctggattttt acaccgggac cgattccgcc
     1021 cgggtcatcg cccggttgac caacgacgcc cagttggttc aaaacgccct gaccaagatc
     1081 cccatcatga tcgtccggga cggtctcacc gtcgtggccc tcaccgggtt cctcttttac
     1141 ctgcattggc gcttcgcctt gatcgcgttc tcgctgctgc ccgtgtcggg ctatctgatc
     1201 gcccgtttcg gcaaaagcct gcgcaaaacc tcccgccaag ggcaggccaa gatgtccgac
     1261 ctctatacgt tgattcagga aaccatcgcg ggcgctccgg tggtcaaggc cttccaacgg
     1321 gaggattacg aaaaagcccg gttcgaggcg gagaaccggg cttactttga catctacatg
     1381 cggaacgccc gggtggagtc cttgtcgagc cccgtcatgg agtttttggg ggccatcggc
     1441 atgggcgtca ttctttggtt cggcggcaag gacgtggtcc accacgtgtg gaccgccggc
     1501 gcctttttcg ccttcatcgg gagcgccctc tcgctctatc agcccgtgaa gaacttttcc
     1561 cggtccaacg cgacgttgca gctcgccttt gccggcgccg aacggatgtt cgagttggtg
     1621 gacgcccgac ccaccgtcgt ggaaaaaccg aacccgaccg atctcgggcc cttttcggat
     1681 cgaatcgatt ttgaccgggt gtcctttgcc taccgttcgg accagcccgt cctaaaggac
     1741 attcagctca cggttcggcg gggggaaatc gtcgccttgg tcggcccgtc gggctccgga
     1801 aagacgaccc tgtcggccct tttgcttcgc ttctacgatc cccggtccgg cgcccttcga
     1861 atcgacggcg tcgaccttcg ggaggccagt tttaagagtc ttcgcgggca gatcggtttg
     1921 gtcacccagg aaacggttct tttcaacgac accatccgcc acaacatcgc ctacgcccgg
     1981 gccgacgcca cggaggccga aatcatcgcg gccgcccagg ccgccaacgc ctgggagttt
     2041 atacgggaca aaccccgggg gttggacacg ctgatcggcg agcggggcgt gcttctctcc
     2101 ggcggtcaaa aacaacggtt ggccctggcc cgggcgatcc tgaccgaccc gcggatcctc
     2161 gtgctcgacg acgccacctc ggcggtcgac gccgccaccg aggaggcgat ccacgcgacg
     2221 ctccggacgc tgatgcaggg ccgcaccacg ctgatcatcg cccac
//
LOCUS       DAOQLS010000330         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31001_length_2258_cov_5.816160, whole genome shotgun sequence.
ACCESSION   DAOQLS010000330 DAOQLS010000000
VERSION     DAOQLS010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31001_length_2258_cov_5.816160"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1211)
                     /locus_tag="PK362_06750"
     CDS             complement(66..1211)
                     /locus_tag="PK362_06750"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNC74563.1"
                     /translation="MGLLYVLKGFFLWFLPLSAGMTWLVLRGPLKAWPAPRRRWLGVF
                     LGIGAFLWSLGRGRAALDAVIAYHVEILTADPANSMAQGYLIGDFVPCRVLLFGGSVP
                     ETVRPVLLKTAPNPRVCGEALAAYNRAKAYRRDLAMWNACGPSRELDVARSAGATTNR
                     PPEDPRSTERVLDAWKRIEIWLARRAPDALATLNPPASQTEMAAAEKEMGLWFPADLR
                     ASLLRHDGQTKAVPAAFPQGLAALSVKGILESWKTRTHRLGAMLGDRDDFDGWRKSIA
                     QGVMFVGGPVKARLADPRWIPIADSNGDVFWFVDLDPAPGGTPGQVIEVDPEGTTWEV
                     LSPSLADHLTRYADALEKGDYTADPQFGQITPRVRRPTPRGLPDYLK"
     gene            complement(1211..>2258)
                     /gene="nadE"
                     /locus_tag="PK362_06755"
     CDS             complement(1211..>2258)
                     /gene="nadE"
                     /locus_tag="PK362_06755"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNC74564.1"
                     /translation="DELVYDGGSLALDAKGRVFAQAPAFEENLLLIDWTAPAGRGQPA
                     FSLPRRSGPRSPSPSVPARPLAPMEEIHRALVLGLGDYVRKNKFSKVLVGLSGGVDSA
                     LVAALAVDALGKDNVVGVTLPSRFNAAATRADARITAENLGIAFHTVPIEKIVGRFRK
                     ALGPLFAGRAPDVTEENLQSRVRGTVLMALSNKFGWLVLTTGNKSELSTGYFTLYGDS
                     AGGFAPIKDLPKGLVYDLCRWRNAHVGPHIPASVLTRAPTAELRKNQTDQDTLPPYPQ
                     LDAVVRGHVEGHGSLKELTAGGVPPAVARRVLRLIDLAEYKRRQAPPGVKITPRAFGR
                     DRRMPITNGFRPWE"
BASE COUNT          402 a          696 c          787 g          373 t
ORIGIN      
        1 caattgaagg gccgtttcgc gtccgatgcc gctggaggcg ccggtgatca aggcgacggg
       61 ggcggtcatt ttagatagtc cgggaggccc cgaggcgtcg gtcgtcgaac ccggggtgtg
      121 atctggccga attgaggatc cgcggtgtaa tcgccttttt ccaaagcgtc cgcgtatcgg
      181 gtcaagtggt cggccaggga gggggaaaga acctcccagg tggtgccttc ggggtcgact
      241 tcaatgactt ggcccggcgt tcctccgggc gcggggtcca aatccacaaa ccagaacaca
      301 tccccgttgg agtcggcgat ggggatccac cggggatcgg ccaagcgcgc cttgaccggt
      361 ccgccgacaa acatcactcc ctgggcgatg gattttctcc agccgtcaaa atcgtcccgg
      421 tcgcccaaca tggcgcccaa tcgatgggtt cgggttttcc aggattccaa aattcctttc
      481 accgaaagag cggccaaccc ctggggaaag gcggcgggga ccgccttggt ttgcccgtcg
      541 tggcgaagga gactcgcccg aaggtcggcg ggaaaccaca ggcccatttc tttttccgcg
      601 gcggccatct ccgtttggga ggccgggggg ttcagagtcg ccaaagcgtc cggcgcccgg
      661 cgggcgagcc aaatttcaat tcgtttccag gcgtcgagga cgcgttcggt cgaacgcggg
      721 tcttccggcg ggcggttcgt ggtcgcgccc gccgatcgag cgacgtccaa ttcgcgcgac
      781 gggccgcagg cgttccacat cgcgagatcc cgccggtacg ccttggcgcg gttgtaggcg
      841 gccaaggctt ctccgcaaac gcgggggttc ggggccgtct ttagcaggac cggccggacc
      901 gtttccggga cacttccacc gaagagaaga acccggcaag gaacaaaatc gccgatcaaa
      961 tagccctggg ccatggaatt ggccgggtcg gccgtcaaga tttcgacgtg gtaggcgatc
     1021 accgcgtcca gggccgcgcg cccgcgcccc agggaccaaa ggaacgcccc gatgcccaaa
     1081 aacaccccga gccagcgtcg acggggggcg ggccaggcct tcagcggccc cctcaaaacc
     1141 aaccacgtca ttcccgccga cagcggaagg aaccagagga aaaaaccctt gaggacgtag
     1201 agaaggccca ttattcccag ggtcgaaagc cgttggtgat gggcatgcgc cggtcccggc
     1261 cgaaggcccg gggcgtgatt ttaacgccgg ggggagcctg gcgccgcttg tattcggcca
     1321 gatcgatcag tcgaagcacc cgtcgggcga cggcgggggg cacgccgccg gccgtcaatt
     1381 ccttcaacga cccgtggcct tccacgtggc cccggaccac ggcgtccaac tgcgggtaag
     1441 gcggcagggt gtcctggtcg gtctggtttt tccgcaactc ggccgtgggc gcccgggtca
     1501 ggacggacgc gggaatgtgg gggccgacgt gggcgtttcg ccaccggcaa agatcgtaga
     1561 cgagtccttt gggaagatcc ttgatgggcg cgaaaccccc ggcggaatcc ccgtaaaggg
     1621 tgaagtagcc ggtcgacagt tccgatttgt tgccggtggt gagcaccagc cagccgaatt
     1681 tgttcgagag ggccatgagg accgtgcccc gcacccggga ttgaagattt tcctcggtga
     1741 cgtcgggggc ccttccggcg aagagcggcc ccagggcttt tcggaaacgc ccgacgattt
     1801 tttcgatggg gacggtgtgg aaggcgatgc ccaggttttc ggccgtgatt cgcgcgtcgg
     1861 cccgggtggc cgcggcgttg aaccgggagg ggagcgtcac cccgacgacg ttgtcctttc
     1921 cgagcgcgtc gacggccaag gccgccacca gggccgaatc cacgccgccc gaaagtccca
     1981 cgaggacttt ggagaatttg tttttacgga catagtcgcc gaggcccagc acgagggccc
     2041 ggtgaatttc ttccataggg gccaggggcc gcgccgggac cgacggcgac ggcgaccggg
     2101 ggccggaacg acgagggagg gaaaaggccg gttggccgcg gcccgcgggg gcggtccagt
     2161 cgatgaggag gaggttttct tcgaaggccg gggcctgggc gaacacccgg cccttcgcgt
     2221 ccagggccag gctcccgccg tcgtacacca gttcgtcc
//
LOCUS       DAOQLS010000331         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31083_length_2255_cov_3.619545, whole genome shotgun sequence.
ACCESSION   DAOQLS010000331 DAOQLS010000000
VERSION     DAOQLS010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31083_length_2255_cov_3.619545"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK362_06760"
     CDS             complement(<1..193)
                     /locus_tag="PK362_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74565.1"
                     /translation="MTLPSRRVFADPPEMARAAAERVADLLRRRGTDRKPLSLVLAGG
                     RSADLFNRFFFNPRRFPSVP"
     gene            complement(190..1728)
                     /gene="zwf"
                     /locus_tag="PK362_06765"
     CDS             complement(190..1728)
                     /gene="zwf"
                     /locus_tag="PK362_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNC74566.1"
                     /translation="MSPSRGKVLRPVSLPAASGPRVSPCGIVIFGASGDLAERKLFPS
                     LFNLHTEGFLPAAFFAVGTGRTALTDDSFRSHVAEALKAILKSPPSAADLDEFLAHFY
                     YQRLDYGNTEDYRGLAARIAELEARHGTGGNRLHYLSIPPSLYETVVERLGEAGLARG
                     GGGGWSRIVIEKPFGRDAGSARVLSEKVWKVFNEDQIYRIDHYLGKETVQNILVLRFA
                     NIFFEPVWNRNFVDHVEITVAEDIGIGHRAGYYEEAGVLRDMFQNHLFQLLCITAMEA
                     PSGFEADRVRDEKSKLLRAVRPFDPARLDETAVRGQYGRGDVAGVLQRAYLEEPGVAP
                     RSKTETFAALKVSIDNWRWEGVPFYLRSGKRLAQRLTEIVVQFKRIPHLMFHPLHAED
                     LSANRLVLRIQPEEGLSLTVETKRPGPALSLGTVGMDFSYEHAFGESPEAYERLFLDA
                     MAGDQTLFTRSDWVDLSWSLLTPVLESWRNAGAPPVYASGSWGPPDADRLLAAQGHRW
                     RSGQ"
     gene            complement(1725..>2255)
                     /locus_tag="PK362_06770"
     CDS             complement(1725..>2255)
                     /locus_tag="PK362_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HNC74567.1"
                     /translation="LQNGYCLMVGGTPDDIARLAPIFTTLAPPEGWLHCGPAGAGHFV
                     KMVHNGIEYAMMQGYAEGFEILRASPYALDLGRISGLWNRGSVVRSWLLELAERAFAK
                     DKDLSGLMDFVQDSGEGRWTVEQAIETGVPAPTIALSLFARFSSQQTESFAGKVLAAL
                     RQEFGGHPVKSKSPGA"
BASE COUNT          467 a          709 c          703 g          376 t
ORIGIN      
        1 agggaaccga cgggaaccgt cgagggttga aaaaaaatcg attgaagaga tcggccgaac
       61 gtccccccgc taaaacgagg gagaggggct tgcggtcggt cccccgacgt cgaaggagat
      121 ccgccacccg ctccgcggcc gctcgggcca tttccggcgg atcggcaaac acccgacggg
      181 agggaagggt cattgcccgc ttcgccaccg atggccttgg gcggccagga gacgatcggc
      241 gtcgggcggc ccccaactgc cggaagcgta gaccggcggt gcgcccgcgt tccgccaact
      301 ttccagaacg ggggtcaaaa gggaccacga gaggtccacc caatccgatc gggtgaaaag
      361 cgtctgatcg ccggccatgg cgtccaagaa aaggcgttcg tacgcctccg gggattcccc
      421 aaaggcgtgt tcgtaggaaa aatccatgcc gacggtgccc aaactcaagg ccggacccgg
      481 ccgcttggtc tccaccgtga gggacaatcc ctcctccggc tgaatgcgga gcaccaaccg
      541 attggccgac aggtcttcgg cgtgaagggg atgaaacatc aaatgcggga tgcgcttgaa
      601 ttggacgacg atctccgtca atcgttgcgc cagccgtttg ccggaccgga ggtaaaacgg
      661 aacgccctcc catcgccaat tgtcgatgga caccttgagc gcggcgaagg tttccgtttt
      721 ggatcgcggc gccacgcccg gctcctccag ataggcacgt tgcaaaacgc ccgccacgtc
      781 gccgcgtccg tattgcccgc gcacggccgt ctcgtccaac cgggccggat caaagggtcg
      841 aacggcgcga agcagtttgg acttttcgtc ccgcacccgg tcggcctcaa accccgacgg
      901 ggcttccatg gccgtgatgc acagaagctg aaataaatga ttttggaaca tgtcccgcag
      961 aaccccggcc tcctcgtaat accccgcccg gtgcccgatg ccgatgtcct cggccaccgt
     1021 gatctcgacg tggtcgacaa aattccggtt ccaaacgggc tcgaaaaaaa tattcgcgaa
     1081 tcgcaggacg agaatgtttt gaacggtctc tttgcccagg taatggtcga tccggtaaat
     1141 ttggtcttcg ttgaagactt tccacacttt ttccgacaac acccgggccg acccggcgtc
     1201 ccggccgaag ggtttttcaa tcacgatacg cgaccagccg ccgcccccgc cccgggccaa
     1261 ccccgcctcc cccagtcgtt ccaccaccgt ctcgtagagg ctcggtggaa tcgacaaata
     1321 gtgcaggcgg tttcccccgg tgccgtggcg ggcctccagt tcggcgattc gggccgccag
     1381 gccccggtaa tcctccgtgt tcccgtaatc caggcgctgg taataaaagt gggcgagaaa
     1441 ttcgtcgagg tccgccgcgg agggcggcga tttcagaatc gccttcaacg cctcggccac
     1501 gtgggaacgg aacgagtcgt ccgtcagagc ggtacggccg gtgccgacgg cgaaaaaggc
     1561 cgccgggagg aacccctcgg tgtgaagatt gaaaagggac gggaaaagtt ttcgttcggc
     1621 caaatcgccg gaggcgccga aaataacgat gccgcagggc gacacccggg ggccggacgc
     1681 ggcgggaagc gacaccgggc ggaggacctt cccccgcgag gggctcatgc gcctgggctc
     1741 ttggatttga cggggtggcc gccgaattcc tggcggaggg cggccaatac tttgccggcg
     1801 aaggattcgg tctgctggga ggaaaatcgg gcaaacaacg acagggcgat ggtcggcgcg
     1861 ggcaccccgg tttcgatggc ctgttccacc gtccagcggc cttcgccgga gtcctgaacg
     1921 aaatccatca atccggaaag atctttgtcc ttggcgaagg cccgttccgc caattcaagc
     1981 aaccaagacc ggaccacgct gccccggttc cataaaccgg aaatgcgccc caggtccaaa
     2041 gcgtaggggg aggcccggag aatttcaaaa ccttcggcgt agccctgcat catggcgtat
     2101 tcgatgccgt tgtggaccat cttaacgaaa tgcccggcgc cggcgggccc gcagtgcaac
     2161 cacccttcgg ggggcgccag ggtcgtaaaa atcggggcaa gacgcgcgat gtcgtcgggc
     2221 gttcccccca ccatgaggca ataaccgttt tgaag
//
LOCUS       DAOQLS010000332         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31276_length_2247_cov_2.682026, whole genome shotgun sequence.
ACCESSION   DAOQLS010000332 DAOQLS010000000
VERSION     DAOQLS010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31276_length_2247_cov_2.682026"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PK362_06775"
     CDS             <1..523
                     /locus_tag="PK362_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74568.1"
                     /translation="EPFALALAGLDWREARALTAWVLWGLARGFPLRWRLRLHPSTDR
                     ERVLRGWPGDLLKRLELDASPSAHDALARAAVAVPVSTSMAVDALALGCRVVVPLFSG
                     GLSLNPLLEDAAVRDVVDGPAALHRTAAERANAPFDPSAAARGRAFVEHYWELDERLP
                     RWTTLLFGGTSHG"
     gene            516..1448
                     /locus_tag="PK362_06780"
     CDS             516..1448
                     /locus_tag="PK362_06780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC74569.1"
                     /translation="MVESPVSTAAPRPAGVPAPGACSLCGNNDPANFTFITDRFRYEG
                     KKYAFRCGACQLVFVVPLMSPEEEREFYEREYGVIYSREKGATPQDLFDKRLPDARRD
                     ARLCEKHLKKTDDCLEVGCASGYFLHTIRDRVRSVTGFETHTELRAYCETIGIPMLKD
                     LGAAKPASFDKIFIFFVLEHIGDPMGFLGALKRLLKPGGQLLVEVPNVDDALLALYDI
                     PALRDFYFTPAHQFYYSKSTLDRVLRKAGFSAVAIEPEQRYDLSNHMHWMLAGKPGGQ
                     GKYNHAFSPELLAAYSQNLKDRYLCDTLFAVASA"
     gene            1457..>2247
                     /locus_tag="PK362_06785"
     CDS             1457..>2247
                     /locus_tag="PK362_06785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC74570.1"
                     /translation="MSRLGHKYERWENGRARRAGIDRTPEEFAETMNYRNAGVLESRE
                     RFLSERCSAAWIRELNGIFQTRLAPNRRALFIGSGLGEHEVPLAIRGFNVVASDIFPE
                     GLEETRRLFPELQIRRLDILNPPAEARGAFDDVVDGGLTFYYDDAAVVAVLKAMKGTV
                     RSGGRVFVVHRYNDTALTWWIDRWVLPLEAFLKNTVQWARRSPARFVRKTHGFRRSVS
                     EMTALAAAAGLRARGVFFAGHGWELNRSALLSRLRFPIPLAGTVDR"
BASE COUNT          358 a          728 c          726 g          435 t
ORIGIN      
        1 cgaacccttc gccctggcgt tggcgggact ggattggcgc gaagcccgcg ccttgaccgc
       61 ctgggtgctg tggggtctcg cccgggggtt tccccttcgc tggcgcctgc ggttgcaccc
      121 ctccaccgat cgggaacggg ttttgcgcgg ctggcccggg gatttgttga agcggttgga
      181 attggacgcc tccccctccg cccacgacgc cctggcgcgg gccgcggtgg ccgttccggt
      241 atccaccagc atggccgtgg acgccctggc cctggggtgc cgcgtggtcg tgccgttgtt
      301 ttccggcggg ctgtcgctca accccctgtt ggaggacgcg gcggtccggg acgtcgttga
      361 tggccccgcc gcgctccacc gaacggcggc cgagcgcgcg aacgcccctt tcgatccgtc
      421 cgcggccgcc cgcggccgcg cctttgtcga acactattgg gagctggacg agcggctccc
      481 gcgatggacc acattgcttt ttggaggaac gtcccatggt tgaatccccc gtttccacgg
      541 ccgccccgcg gccggccggc gtgccggccc ccggcgcctg ctccctgtgc ggcaacaacg
      601 acccggcgaa tttcacgttc atcaccgacc ggttccgcta cgaaggcaaa aaatacgcct
      661 tccggtgcgg ggcctgccag ctggtgttcg tcgtccccct gatgagcccc gaggaggaac
      721 gggaatttta cgagcgggaa tacggcgtca tttacagccg cgagaaaggg gccacgcccc
      781 aggacctctt cgacaagcga ctgcccgacg cccgccggga cgcgcgcctg tgcgaaaaac
      841 atttgaagaa aaccgacgac tgcctggagg tcggctgcgc ctcggggtat ttcctccaca
      901 cgattcggga ccgcgtgcgc tcggtgaccg gtttcgaaac ccacacggag ctccgcgctt
      961 attgcgaaac catcggcatc cccatgttga aggacctggg cgcggccaag cccgcgtcct
     1021 tcgacaaaat cttcattttc ttcgtcctgg aacacatcgg ggaccccatg ggctttctgg
     1081 gggcgctcaa gcgcctgctc aaacccgggg gtcaactgct ggtcgaggtg ccgaacgtcg
     1141 acgacgctct cctggcgctc tacgacattc cggcgctccg ggatttttat tttaccccgg
     1201 cgcaccagtt ctactattcc aaatcgaccc tcgaccgggt gttgaggaag gccgggttct
     1261 ccgccgtggc catcgaacct gaacagcgct acgacttgtc gaaccacatg cactggatgc
     1321 tggcgggcaa accgggcggc cagggaaaat acaaccacgc cttctcgccg gagctgctcg
     1381 ccgcctactc gcagaacctg aaagaccgtt atctgtgcga cacgttgttt gcggtggcct
     1441 ccgcctgatc caggccatgt cccgtctcgg gcacaaatac gaacgctggg aaaacgggcg
     1501 cgcgcgccgg gccgggatcg accggacccc ggaagaattc gccgagacca tgaattaccg
     1561 gaacgccggc gtcttggaaa gccgggagcg atttttgtcc gagcggtgct cggccgcgtg
     1621 gatccgggaa ctgaacggaa tttttcaaac ccgtttggcg ccgaaccgtc gcgcgttgtt
     1681 catcggctcc ggactgggcg aacacgaagt gcccctggcg atccgggggt tcaacgtggt
     1741 cgcgtcggat atttttccgg agggcctcga ggaaacccgg cgtcttttcc cggagctcca
     1801 aattcgccgg ctggatattt tgaatccccc cgccgaggcg cggggggcct ttgatgacgt
     1861 ggtcgacggc gggttgacgt tttactacga cgacgcggcc gttgtggccg tgttgaaggc
     1921 catgaaggga accgttcgtt ccggggggcg ggtttttgtc gtccaccgct acaacgatac
     1981 ggcgctgacg tggtggatcg accgatgggt tttgcccctg gaggcgtttc tgaaaaacac
     2041 ggtccaatgg gcgcgacggt cgcccgcgcg gtttgtccga aaaacccatg gatttcggcg
     2101 gagcgtttcc gaaatgacgg cgttggccgc ggccgcgggc ttgcgcgccc ggggagtctt
     2161 ttttgccggt cacggctggg aactcaaccg aagcgcgctc ttgtcccggt tgcggtttcc
     2221 gatccctctg gcgggaaccg tcgatcg
//
LOCUS       DAOQLS010000333         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31280_length_2247_cov_2.271898, whole genome shotgun sequence.
ACCESSION   DAOQLS010000333 DAOQLS010000000
VERSION     DAOQLS010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31280_length_2247_cov_2.271898"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..904)
                     /locus_tag="PK362_06790"
     CDS             complement(146..904)
                     /locus_tag="PK362_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNC74571.1"
                     /translation="MLTQAQKDTYEKTGFVVLRGFLSPRIVRDCLDEITAVFLDQAKK
                     MNRLPSAGNDLDDLVRSVMVPGTPERSFVYDFVRNIRPLRVVETLPEVREVLRDLGLE
                     QPICLEIPSLRFDTPGEETFLTKHHQDIRSIRGERCVTVWFPLRPCGPRHGSLAVLEG
                     SHRLGLLKHVIEDRHVRVADLGALPPDRYPRRIVEGDAGDIVFMNSFCVHVSHPNPSD
                     AIKINGQFFYNDARAVRFGDVFESLKSVPDYRAL"
     gene            complement(898..>2247)
                     /locus_tag="PK362_06795"
     CDS             complement(898..>2247)
                     /locus_tag="PK362_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNC74572.1"
                     /translation="HYSTVYGRPPTFFRDVHQIAPATVLSWAGGAPAEKVYWRFDAAQ
                     SFLPETDEKALADELAARLEKTVEERLAPLPARRGFSLSGGMDSNTILGFAKKLAGEK
                     QVAVSVTYHEDDRANEHSLIAPVAKTSLSVWHNIKPTPAEFRRDMETLYRFYDQPWTT
                     ATVYAHERLSRAAAEAGLTCLVGGAGGDYHMAGNYPNFLYYFAHLRQKKDETLLRREV
                     AKWIENHSTPQWPKSMATVERFFESSVDLRTPGVLRQGDVRLAPGRPLLTASAERLYA
                     LPAPVESYGDYLRSFMVQEIARDALPPPVWAEDVMAWRYGLASADPFWDRALFDFTWR
                     LPHGLKIRDGVNKHLLRRATDGIVPDHLRLRKDKTGFSLPFDRWLEGDLGDFARDVLG
                     SASFAARDIVDPAVARGLLEEHRAGRARHGMVIWQMLNMELWFREWIDPPEREVTLC"
BASE COUNT          414 a          725 c          721 g          387 t
ORIGIN      
        1 gtccagacac accaggccgc aggaccggca aatcaagacg tccaggggaa cggccgcgtg
       61 ccgggagctc ggtttgttga aattcttgaa cacccattcg aaacgttcgg ccccgcaaat
      121 gttgcagcgc atggatcgat tctcctcaga gcgcccggta atcgggcacg gatttaagag
      181 attcaaaaac gtcgccgaac cgcacggcgc gggcgtcgtt gtaaaaaaat tgcccgttga
      241 tcttgatggc gtcggacgga ttcgggtggg aaacgtgaac gcaaaaagag ttcatgaaca
      301 cgatgtcccc cgcgtccccc tcgacaatcc ggcgggggta gcggtccggc ggaagcgccc
      361 ccaggtcggc cacccggacg tggcggtcct caatgacgtg cttcagcagg cccagccggt
      421 ggcttccctc cagcacggcc agggacccgt gccgcggccc gcaggggcgg agcggaaacc
      481 acaccgtcac gcatcgctcg ccccggatgg aacggatgtc ctgatggtgc ttcgttaaaa
      541 acgtttcctc gcccggcgtg tcgaagcgca ggctcggaat ttccaggcag atgggttgct
      601 ccaggcccag gtcccgcagc acctcgcgga cctccggcag ggtttcgacc acccgcaggg
      661 gtcggatgtt gcggacgaag tcgtacacga aggaccgttc gggggtcccc gggaccatca
      721 cggatcgaac gagatcgtcc aaatcgttcc ccgccgaagg aagtcggttc attttcttgg
      781 cttggtcgag aaacacggcg gtgatttcgt ccaggcagtc ccggacgatc cgcggggaca
      841 ggaacccccg aaggaccacg aaccccgttt tttcgtaggt gtctttttgg gcctgcgtca
      901 gcacagcgtc acctcccgtt cgggcggatc gatccactcc cggaaccaca actccatgtt
      961 caacatctgc caaatcacca tgccgtgccg cgcgcggccc gcccggtgct cttccaggag
     1021 gccccgggcc actgccgggt cgacgatgtc ccgggcggcg aacgacgccg acccgagcac
     1081 gtcgcgggcg aaatccccga gatcgccctc cagccaccgg tcgaagggaa ggctgaaacc
     1141 cgttttgtcc tttcgaagcc gcaggtggtc gggaacaatc ccgtccgtgg cgcggcgaag
     1201 caggtgcttg ttcaccccgt cccgaatttt caggccgtgg ggaaggcgcc aggtgaaatc
     1261 gaacagggcc cggtcccaga agggatcggc cgacgcgagg ccgtaacgcc aggccatcac
     1321 gtcttccgcc cagacgggag gcggcagggc gtcccgggcg atttcctgaa ccatgaagct
     1381 gcgcaaataa tcgccgtacg actcgaccgg cgccggcaac gcgtagagcc tctccgccga
     1441 cgcggtcaac aacgggcggc cgggcgccag gcggacgtcg ccctgccgga ggacgccggg
     1501 cgttcgaaga tccacggacg attcaaaaaa ccgctccacg gtcgccatgg atttcggcca
     1561 ttggggggtc gaatggtttt caatccattt ggccacctcc cggcgtaaaa gcgtttcgtc
     1621 ctttttttgc cgaaggtggg cgaagtaata aaggaaattc gggtagttgc cggccatgtg
     1681 gtagtccccg cccgccccgc cgacgaggca cgtcaacccg gcctcggccg cggcccggct
     1741 caaccgctcg tgggcgtaga ccgtcgccgt cgtccacggc tggtcgtaaa accggtacag
     1801 cgtctccatg tcccgccgga actccgcggg ggtgggcttg atgttgtgcc agacgctcag
     1861 cgaggttttc gcgaccggtg cgatcaacga gtgctcgttg gcccggtcgt cctcgtggta
     1921 ggtgaccgac acggcgactt gtttttcccc cgccaatttc ttggcgaatc cgagaatcgt
     1981 gttggaatcc atccccccgc tgagggagaa cccgcgccgg gccgggaggg gcgccagccg
     2041 ctcttcgacg gtcttttcga gccgcgcggc caattcgtcc gccagggcct tttcgtcggt
     2101 ttcgggcagg aacgattgcg ccgcgtcgaa gcgccaatag accttttcgg cgggcgcgcc
     2161 ccccgcccag gacagcacgg tggcgggggc gatttgatgg acgtcgcgaa agaacgtcgg
     2221 gggccgcccg tacaccgtgg aatagtg
//
LOCUS       DAOQLS010000334         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31320_length_2245_cov_4.706849, whole genome shotgun sequence.
ACCESSION   DAOQLS010000334 DAOQLS010000000
VERSION     DAOQLS010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31320_length_2245_cov_4.706849"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..698)
                     /locus_tag="PK362_06800"
     CDS             complement(123..698)
                     /locus_tag="PK362_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNC74573.1"
                     /translation="MTEPSAESAAAEKDAVEAKISELDILRQSLQAAQAKSADYYDQL
                     LRLKAEFENFRKRTEKDRADSRRWGKEEIVLRLVSLLDVMELAEAAAHKSPDVKSMVQ
                     GLDMLYGEFKRLLKEEGLEEIPTAVGDAYNHAAHEAVETIEDKEDGKILAVLQKGYKF
                     QGGLFRPARVKVSRRGPEPRPEKSPDDAPAI"
     gene            complement(695..1768)
                     /gene="hrcA"
                     /locus_tag="PK362_06805"
     CDS             complement(695..1768)
                     /gene="hrcA"
                     /locus_tag="PK362_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNC74574.1"
                     /translation="MRTLSTIEQEDRKRKLLQAIIHQYIKTAKPVGSAFIAGQKHLDL
                     SPATIRNIMGELEREGYLTHPHTSAGRVPTDKAYRFYVDSLVELQKLAIKEEERLRRD
                     YQSRAREIEELMLSTSKTLSAMSQYTGFVLTPAIDQERLQHVELIALEPRRVLAVLVS
                     DTGVVKHRQVVFERPYPREFLEELTRLLNERLRGKPFTDVRDTLLDHIEAIHQRQMDL
                     LDVARRITQQAFDLSTAGDLYLEGTGNILSLPDFSGQEDLRSLVHLLDEKRTLGEMIV
                     RDMADDAPKFSGVRVKIGSENKSPDLKNLSLVSSTYTVGGRQMGVLGIIGPKRMEYSR
                     MISLVNHVAQIVSQTLSRRLEDQ"
     gene            complement(1921..>2245)
                     /locus_tag="PK362_06810"
     CDS             complement(1921..>2245)
                     /locus_tag="PK362_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74575.1"
                     /translation="LTALVRAEGSAKGGKAGAVRANRGERMEKHGEHRQKRGEKMQER
                     GEKMQKAADKAEANGNEKAAERLEKRGEHLENRGERMERQGERMERRGERKQNGVGHT
                     EDSAI"
BASE COUNT          350 a          718 c          673 g          504 t
ORIGIN      
        1 ataccgatga ttttagccat gattgtttcc tcctgaaaaa ttgttccgct tgcttcggtt
       61 ccaccccctc cctcaccctc ccccctcgag ggggagtgac ggggtggggg gcacaacttc
      121 ttttaaatcg ccggcgcgtc gtccggggat ttttcgggcc ggggttccgg gccccggcgg
      181 gagaccttca cccgcgcggg acggaacagc ccgccctgaa acttgtagcc cttctgcagg
      241 acggccagaa tcttgccgtc ctccttgtct tcgatggtct ccaccgcttc gtgggccgcg
      301 tggttgtagg cgtcccccac cgcggtggga atctcctcca ggccctcttc cttgagcagg
      361 cgcttgaact cgccgtagag catgtccagg ccctgcacca tggatttgac gtcggggctt
      421 ttgtgggccg ccgcctccgc caactccatc acgtccagga gcgacaccag gcggaggacg
      481 atttcttcct tgccccaccg ccgggaatcc gcccggtcct tttccgtgcg tttccggaaa
      541 ttttcaaact cggccttgag ccgaagcagc tggtcgtaat aatccgccga cttcgcctgg
      601 gccgcctgca gggactgccg caggatgtcc agttctgaaa tcttggcttc gaccgcgtct
      661 ttttccgcgg cggccgattc ggccgatggt tcggtcattg gtcctccaga cggcggctca
      721 aggtttggga cacgatctgg gccacgtggt tcaccaggct gatcatgcgg gaatattcca
      781 tgcgcttggg gccgatgatg cccagaacgc ccatttgccg gccccccacc gtgtaggtgg
      841 aagacacgag gcttaaattc ttgaggtccg gggatttgtt ctccgaaccg atcttgaccc
      901 tcacgccgga gaatttgggc gcgtcgtccg ccatgtcccg gacgatcatt tcgcccaggg
      961 tgcgcttttc gtccagcagg tggaccaggg agcgcaggtc ctcctggccg ctgaaatccg
     1021 ggagggacag gatgttgccc gtcccctcca aatagagatc cccggcggtg gacaggtcga
     1081 aggcctgctg ggtgatgcgc cgggccacgt ccagcaaatc catctgccgc tggtggatgg
     1141 cctcgatgtg gtccaagagg gtgtcccgaa cgtcggtgaa gggcttgccc cgcaggcgct
     1201 cgttcaacag ccgggtgagc tcctccaaaa attcccgggg gtagggccgc tcgaaaacga
     1261 cttggcgatg cttgaccacg ccggtgtcgc tcacgagcac ggctaggacc cgccggggct
     1321 ccagggcgat cagctccacg tgctgcagac gctcctggtc gatggccggc gtcagcacga
     1381 agccggtgta ttgcgacatg gccgaaaggg ttttggaggt cgagagcatc agctcctcaa
     1441 tttcccgggc ccgggactgg tagtcccgcc gaaggcgctc ttcttcctta atggccagct
     1501 tctggagctc caccaggctg tccacgtaaa atcggtaggc cttgtccgtg ggcacccggc
     1561 cggcggaggt gtgggggtgg gtcaaatacc cttcgcgctc caactctccc atgatgttcc
     1621 ggatggtggc cggggaaagg tccaaatgct tttgccccgc gataaaggcg gaccccaccg
     1681 gtttcgcggt cttgatgtat tgatggatga tcgcctgcag aagcttccgc ttgcggtctt
     1741 cctgttcgat ggtcgagagc gttcgcattt tttggggccc cggttttaat tagcactcaa
     1801 aaaccgggag tgctaacagt ataacacagg ccggatttcc tgtcaattac gggaaggatc
     1861 gacggaaaaa gaaaaccccc ggggccggag ccgccggggg ttttcccgtg gaaagaatcg
     1921 ttaaatggcc gagtcttcgg tgtggcccac gccgttttgc ttgcgttcgc cccggcgttc
     1981 catccgttcg ccctggcgct ccatccgctc gccccggttt tccagatgct ctccgcgttt
     2041 ttccaagcgt tcggcggctt tctcgttgcc gttggcttcg gccttgtccg cggctttctg
     2101 cattttctcc ccgcgctcct gcatcttctc gccgcgtttc tgccgatgct cgccgtgctt
     2161 ttccatgcgt tctccgcgat tcgcccgcac cgcgcccgcc ttgccgcctt tggccgaacc
     2221 ctccgcccgg accaacgccg tcagg
//
LOCUS       DAOQLS010000335         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31516_length_2237_cov_4.208983, whole genome shotgun sequence.
ACCESSION   DAOQLS010000335 DAOQLS010000000
VERSION     DAOQLS010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31516_length_2237_cov_4.208983"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PK362_06815"
     CDS             <1..304
                     /locus_tag="PK362_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74576.1"
                     /translation="NFWTDGIGHMACAYYAVGDDARGNFYANQMDALVMDRTVGDKAV
                     RCLPYTANAEGGYEWVKFDRGFLSSAAWYIFAKNRFNPFTLRVAAPAADAYADRGR"
     gene            408..2165
                     /locus_tag="PK362_06820"
     CDS             408..2165
                     /locus_tag="PK362_06820"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNC74577.1"
                     /translation="MIPYRMTRENSGHVVETPLAGQMLLDTPLLNKGSAFTAEERRSF
                     GLDGLLPPHISTWEEQLLRTYGNFQQKTTDLERYVFLLALQDRNETLFYGLVQAHIQE
                     MMPIIYTPVVGAACQHFSRMFRRPRGLFLSYPDRDRMDAMLANVTNPAVDVIVVTDGQ
                     RILGLGDLGMNGMGIPVGKLSLYTLCAGIHPATTLPILLDVGTDNPALLNDPLYLGWR
                     HERVKGAEYDAFIEQFVRAVMKRWPQVLLQWEDFSKGNASRLLDRYRDTLCTFNDDIQ
                     GTGAVTAAGILAAVKAAGRPIADERVVILGAGSAATGIGDQIVLAKEKAGVPADQARR
                     SLWLIDTQGLVHTGRAEMEETKKRFAQPRESLSGWTLDRPGEYFLADVVRNVKPTALI
                     GTSAQPGVFTEPIVRDMARAVDRPIVFPLSNPTSKCEALPADLLRWTDGRALVATGSP
                     FAPVEVKGSPVKIGQCNNAYIFPGMGLGVVASRARRVSDRMFFAAAETLADLSPALED
                     PAAGLFPDLTSIRSVSHAVALAVARQAQREGLADKISEAELEERVSAKVWVPRYVPYR
                     RGLAADFAKTRSAEPASRS"
BASE COUNT          377 a          776 c          718 g          366 t
ORIGIN      
        1 caacttctgg accgacggca tcgggcacat ggcctgcgct tactacgccg tgggggacga
       61 cgcccggggg aacttctacg ccaaccagat ggacgccctg gtgatggacc gcaccgtcgg
      121 cgacaaggcc gttcggtgcc ttccctacac cgccaacgcc gagggcggct acgaatgggt
      181 caagttcgac cggggctttc tgtcttcggc cgcctggtac attttcgcca aaaaccgatt
      241 caatcccttc accctccggg tggcggcccc cgcggccgac gcctacgccg accggggccg
      301 ataaccccca accctgacgc ccctcattga cagggcgccg cgcgtccggt cagaatgggg
      361 gcgttgttaa agcccggccc cttccgggac cgccccagga gaaccccatg attccctacc
      421 gaatgacccg cgaaaattcc ggccacgtcg tggagacccc cctggcgggt caaatgctgt
      481 tggacacccc cctgttgaac aaaggaagcg ccttcacggc ggaggagcgc cggtccttcg
      541 gcctggacgg ccttcttccg ccccacatct cgacctggga agaacagctg ctccgcacct
      601 acggcaattt ccagcagaaa accacggacc tggaacgcta cgtgtttctc ctcgccctcc
      661 aggaccggaa cgaaaccctc ttttacggcc tggtgcaggc ccacatccag gaaatgatgc
      721 cgatcatcta cacccccgtg gtgggcgccg cctgccaaca tttcagccgg atgttccgcc
      781 gaccccgggg gcttttcctt tcctatcccg accgcgaccg gatggacgcc atgctggcca
      841 acgtgacgaa ccccgccgtc gacgttatcg tggtgaccga cggccagcgc atcctcgggt
      901 tgggcgattt gggcatgaac ggcatgggga ttccggtggg caaactttcg ctttacacgc
      961 tctgcgcggg catccatccg gcgacgacgc tgccgatcct tttggacgtc ggcaccgaca
     1021 accccgcgct cctgaacgat ccgctgtacc tgggttggcg ccacgaacgg gtgaagggcg
     1081 cggagtacga cgcattcatc gagcaattcg tccgggccgt gatgaaacgg tggccccagg
     1141 tccttcttca atgggaggat ttttccaagg gcaacgcgtc ccggctgctg gaccgttacc
     1201 gcgacaccct gtgcacgttt aacgacgaca tccaaggcac cggcgcggtg acggccgcgg
     1261 ggatcctggc cgcggtcaaa gcggccggcc gtcccatcgc cgacgaacgg gtggtcattc
     1321 tgggggcggg gtccgcggcc acgggcatcg gggatcaaat cgtcctggcc aaggaaaagg
     1381 ccggggtgcc ggcggaccag gcccggcggt ccctgtggct gatcgacacc caggggttgg
     1441 tccacaccgg gcgggccgaa atggaagaaa cgaaaaagcg gttcgcccaa ccccgggaga
     1501 gcctctccgg gtggaccctg gaccggcccg gcgaatattt cctggccgac gtggtgcgga
     1561 acgtcaaacc caccgccttg atcggcacct ccgcccagcc gggcgtgttt acggaaccca
     1621 tcgtgcgcga catggcccgc gcggtggacc ggccgatcgt tttccccctg tccaacccca
     1681 cgtcgaagtg cgaagccctg ccggcggacc tgctccggtg gaccgacggg cgggccctgg
     1741 tggcgaccgg cagtcccttc gccccggtgg aggtcaaagg atccccggtc aaaatcgggc
     1801 agtgcaacaa cgcttatatt tttccgggaa tggggttggg ggtcgtggcc agccgggcgc
     1861 gtcgggtgtc ggaccggatg ttcttcgccg cggcggaaac cctggcggat ttgtccccgg
     1921 ccctggaaga ccccgccgcg ggcctgtttc cggacctgac ctccatccgg tccgtgtccc
     1981 acgccgtggc cctcgccgtg gcgcgccagg cccagcggga aggattggcc gacaaaattt
     2041 cggaggccga gctggaagag cgggtatcgg cgaaagtctg ggtgccgcgc tacgtgccct
     2101 accggcgggg cctggccgcg gatttcgcca agacgcggtc cgccgaaccc gcctcccgct
     2161 cctaacgggg ccgggaaggc ccggggcgcg gccgcgccgc gccgttgacc accctgcggc
     2221 cctgcgctag cgtgcgc
//
LOCUS       DAOQLS010000336         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31580_length_2234_cov_4.552088, whole genome shotgun sequence.
ACCESSION   DAOQLS010000336 DAOQLS010000000
VERSION     DAOQLS010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31580_length_2234_cov_4.552088"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PK362_06825"
     CDS             complement(<1..461)
                     /locus_tag="PK362_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011909614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC74578.1"
                     /translation="MKTEFDVIVVGAGHAAVEAALASARLGRSTALVTLDRTRIGQMS
                     CNPAIGGVGKGQVVREIDALGGEMARATDRAGLQFKMLNRGKGPAVLSPRAQCDRSLY
                     RAVVTEAVLRQPGLTVLEGEVVDLLVESGAAAGVRIRDGSPLRSRAVVIAAG"
     gene            complement(540..1106)
                     /locus_tag="PK362_06830"
     CDS             complement(540..1106)
                     /locus_tag="PK362_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNC74579.1"
                     /translation="METIGASFGLVAASEMGDKTQLLALSLAARFKKPVPILGGILIA
                     TVLNHGLASTAGSWISTHVSASLLAGILGVTFIAFGLWTLKPDDLEEPKESRWGPFLT
                     TVVLFFLAEMGDKTQLATVALAAKYGSVWWVTFGTTLGMLAADGAAVFFGEAISRRLG
                     MKWIRWPAAALFFLFGGLSLYRAFSIRS"
     gene            1272..2072
                     /locus_tag="PK362_06835"
     CDS             1272..2072
                     /locus_tag="PK362_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74580.1"
                     /translation="MLKSYGPMVLPDTPPPPVSAAETEAALKAALVASEKYGRFNHRR
                     LEALESLAEFLVRRERWLEAEPLKLQILSAKEKLYGPEHQSLAPTMAELAVGYEAMGR
                     FDQAEAVLTLALKINERDAMQNTAAIAGNLAGLGRLEAIRGRWSEAETHLVRALKITR
                     IVHGPDHTEMARLLTRWGEAALEGGQTMAAAAKLEEADRIYTHALGAAHPDTANVWVQ
                     RGQWARRMGRWAEAESFYRRAMAVHEHREGLALAGVSDDAAHRPVSLR"
BASE COUNT          458 a          724 c          699 g          353 t
ORIGIN      
        1 ccggccgcga tcaccacggc ccgggaccgg agaggagacc cgtcgcgaat tcgaacgccc
       61 gcggccgccc cggattccac caatagatcc accacctcgc cttccaggac ggtgagaccc
      121 ggttggcgga gaaccgcttc ggtcacaacg gcacggtaca aacttcggtc gcattgggcc
      181 cgggggctca agaccgcggg tcccttgccc cggtttagca tcttaaactg cagaccggcc
      241 cgatccgtgg ctcgggccat ttcaccgccc aaagcgtcaa tttcccggac cacctgcccc
      301 ttgcccacgc cgccgatggc cgggttgcag gacatttgtc cgattcgagt tcgatccaaa
      361 gtgacgagcg cggttgatct tccaaggcgc gccgaagcca acgcggcctc cacggccgcg
      421 tggccggcgc caacgacaat gacgtcaaac tcggttttca tatctataaa tcataccatt
      481 ttcgcattaa aattcccgga ttcaatcacc aagaaccgcc gatgggggga aggggaacct
      541 taagatcgga ttgaaaaagc gcggtaaagg gagagaccac cgaaaagaaa aaacagtgcg
      601 gccgccggcc aacggatcca tttcatgcca aggcgccggg aaatggcttc gccgaaaaac
      661 accgccgcgc cgtcggccgc caacatgccg agggtcgtgc cgaaagtcac ccaccacacg
      721 ctcccgtatt ttgcggccag ggccacggtc gccagctggg ttttgtcgcc catttcggcc
      781 aggaaaaaaa gcaccaccgt tgttaaaaaa ggcccccaac gggattcctt gggttcttcc
      841 aaatcatcgg gtttcaacgt ccacaaccca aaggcgatga aggtgacgcc aagaatcccg
      901 gccaacaacg aagccgaaac gtgggtggaa atccagcttc cggcggtgga ggccaaccca
      961 tggtttagaa ccgtcgcgat caagatgccg cccaggatgg ggaccggttt tttgaaacgg
     1021 gcggcgaggg aaagggcgag cagttgggtt ttgtcgccca tctcgctggc cgccacaaga
     1081 ccgaaggaag cgccgatggt ttccaggaaa ggcatggcag gagagcctaa ggaatttcga
     1141 cggtttcgtc aatgacggcc gccggccgcg tcccgggttc gcgggacgga cgggggcggg
     1201 gaatcccctt gtccttcatg gtttctcccg gtgttttcgc ccgtcgaaag ttcaccgacg
     1261 acgggccgtc tatgctaaaa tcgtacgggc cgatggtcct tcccgacacc cctcccccgc
     1321 cggtttcggc ggcggaaacc gaagccgcgc tcaaggccgc gctggtggcg tcggagaaat
     1381 acggccgctt caatcaccga cggctggaag ccctggaatc cctggccgag tttttggtgc
     1441 gccgcgaacg gtggctggag gcggaacccc tcaaacttca aattttatcg gccaaagaaa
     1501 aactttacgg accggaacat caatccctgg ccccgacgat ggccgaactg gccgtcgggt
     1561 atgaagccat gggccgcttt gaccaggccg aggcggtgct gaccctggcg ctcaaaatca
     1621 acgagcggga cgccatgcaa aacacggccg ccatcgccgg caatctcgcc ggcttgggaa
     1681 ggttggaggc catccgcggg cgctggtcgg aggccgaaac ccatttggtc cgggccctta
     1741 aaatcacccg gatcgttcac gggcccgacc acacggaaat ggcgcgcctc ctcacccgtt
     1801 ggggggaagc ggccctggaa ggcggccaaa ccatggccgc cgcggcgaag ctggaagagg
     1861 ccgaccggat ttacacgcac gcgctcggcg ccgcccaccc cgacacggcc aacgtgtggg
     1921 tgcagcgcgg ccaatgggcc cgccgcatgg ggcgatgggc ggaggcggaa agcttttacc
     1981 ggcgggcgat ggccgtccac gagcaccggg agggcctcgc cctcgccggt gtatccgatg
     2041 atgccgcaca tcgtccggtc agcctccggt gacggtcgcc tcgaggggga cgcgcagctc
     2101 gggcacctcg gcgtcgtcca ccgagaccac cagcgtcgcg gtgacgcgac cgaggtcgcg
     2161 cgggcgcccg tcgtagcgga cgtccaccgc ccagccttcc ttgacgcggc gcggcgggtc
     2221 ggcgacgaag cctt
//
LOCUS       DAOQLS010000337         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31668_length_2231_cov_3.516085, whole genome shotgun sequence.
ACCESSION   DAOQLS010000337 DAOQLS010000000
VERSION     DAOQLS010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31668_length_2231_cov_3.516085"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1240)
                     /gene="arsB"
                     /locus_tag="PK362_06840"
     CDS             complement(188..1240)
                     /gene="arsB"
                     /locus_tag="PK362_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNC74581.1"
                     /translation="MTIPLASVKKLSFLDRYLTLWIFLAMAGGVLFGGVLPAVGRFVG
                     DLKVGTTNIPIAVGLILMMYPPLAKVKYERLGDVFRNKRVLGLSIFQNWAVGPVLMFL
                     LAAVFLRNRPEYFAGLVLIGLARCIAMVIVWNELAEGDTEYAAGLVAFNSVFQVAFYS
                     VYAYFFLTVLPPLVGLQGTTVFVPMGTIAESVIIYLGIPFLAGFVTRWVALKTKGADW
                     YEMVFLPRIAPITLTALLFTILMMFSMKGGEILRIPLDVGRVAIPLLLYFVGMFLVSF
                     WLGKKLGADYSKTATLSLTAASNNFELAIAVAVAVFGLHSGAAFAAVIGPLVEVPVLV
                     GLVKVAHFFRTRYFVS"
     gene            complement(1237..>2231)
                     /locus_tag="PK362_06845"
     CDS             complement(1237..>2231)
                     /locus_tag="PK362_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74582.1"
                     /translation="QRSFYWHSPLADAAQDYLKGKRKPKKGTAVDAARQIYKRGTEIY
                     DLWCAHDWGLLELAQDPQAGETKMVTLYAEQLTALEERNKKINMGLKSVWPDGMAYFP
                     MMFKVIRAYFYGWGVPVDLEKAQPFVKKMEFSYGILWGDLDVRTVDKTDPAKRHLWVD
                     AQALQAEMRALGFGFYGKPNLDYARYWIRRAFETIEGQPDLYTVDDKLTLLLTLLSFD
                     GDLDNKELSFNIVKEGYDHNPLLAGGEYVYKLLKGDGAPANPEEAYKIATDLPAMEPK
                     ALSRVVRLWLGNDGVARDTAKAKELLDRFSDPANPEHARLSQEVLAPPAAATPL"
BASE COUNT          567 a          637 c          574 g          453 t
ORIGIN      
        1 agtaaacaga atgagaggcc accacaaaag gaaaaaggac aaaacgattt gaatgaggat
       61 tttcatgggt ttaggataag acatccgttg ggcggtcgca atggatcctt cggcggcctc
      121 taccctcttc cgacaaattc tgtttgctcc atacacgatc cagcggaatg gatcggcccg
      181 ttaatcgtca agagacaaaa taacgtgttc taaaaaaatg ggcgactttc acaagcccca
      241 ccaggacggg gacttcgacc aacggcccaa tgaccgccgc gaaggccgcc ccggaatgga
      301 ggccgaacac cgcgacagcc acggcgatgg ccaattcaaa gttattgctc gcggcggtga
      361 gggacaacgt cgcggttttg gagtaatccg cccccaactt ttttcccaac cagaaactga
      421 ccaggaacat ccccacgaaa tacagcaaca ggggaatcgc cacccgcccc acgtccaaag
      481 gaatcctcaa tatttccccg cccttcatgc tgaacatcat caggatcgta aaaagaaggg
      541 cggttaaggt gatgggggca atacggggaa ggaaaaccat ttcgtaccaa tccgccccct
      601 tcgtttttaa agcgacccac cgggtcacaa accccgctaa aaacggaatg cccaaataaa
      661 tgatgacgct ttcggcgatg gtccccatgg gaacgaaaac cgttgttcct tgcaaaccaa
      721 cgaggggagg aagaacggtc aaaaagaaat aggcgtagac gctgtaaaaa gccacctgaa
      781 aaacgctgtt aaaggcgacc aatcccgcgg cgtattccgt gtcgccttcg gccaactcgt
      841 tccaaacgat aaccatggca atgcagcgag ccaaaccgat gagcaccaac cccgcgaaat
      901 actccgggcg gttccttaaa aacaccgccg ccaggagaaa catcaaaaca gggcccaccg
      961 cccaattctg gaaaatggac aaacccaaca cccgtttgtt tcgaaacacg tcccccagcc
     1021 gctcgtattt gaccttggcc aacggaggat acatcatcaa tatcaacccc accgcaatag
     1081 ggatgttggt ggtgccgact ttaagatccc ccacaaaacg cccgaccgcg ggaaggaccc
     1141 cgccgaaaag caccccgccg gccatggcca agaaaatcca aagcgtcaaa tagcgatcca
     1201 ggaaggaaag tttcttaacc gaggccaagg gaatcgtcat aaaggcgtcg ccgccgcggg
     1261 cggcgctaaa acttcctgac tcaaacgagc gtgttccgga ttggcggggt cgctgaagcg
     1321 atcgaggagt tccttggcct tggcggtgtc ccgggccacg ccgtcgttgc ccaaccaaag
     1381 acgcacgacg cgcgacaggg ctttcggttc catggcgggc aggtcggtgg cgattttgta
     1441 ggcctcttcg gggttggcgg gggcgccgtc gcccttcagc aatttgtaga cgtattcgcc
     1501 ccccgcgagg agcgggttgt ggtcataccc ttctttaacg atattgaacg aaagctcttt
     1561 gttgtccaag tccccatcga agcttaacag agtcaggagc aaggtcaatt tatcgtcaac
     1621 agtgtagagg tcgggttggc cttcgatggt ttcaaaggcg cggcgaatcc aataccgagc
     1681 gtaatcaaga ttcggttttc catagaaccc aaaccccaat gctcgcattt ccgcctggag
     1741 ggcctgcgca tcgacccaaa gatgtcgttt ggccggatcc gttttatcaa cggttctgac
     1801 atccaggtcc ccccaaagga tgccgtaact aaattccatt ttctttacaa agggttgtgc
     1861 cttttccaaa tccaccggca cgccccagcc gtagaagtag gctcggatca ccttgaacat
     1921 catggggaag taggccatcc catcgggcca gacggatttt aaccccatat tgattttttt
     1981 gtttcgttcc tccagggcgg ttaactgttc cgcgtaaagg gtcaccattt tggtttcccc
     2041 ggcctgggga tcctgcgcca gttcaaggag tccccaatcg tgggcgcacc acaggtcgta
     2101 tatttccgtt ccgcgtttat aaatttgccg cgccgcgtcg accgcggtgc cctttttggg
     2161 tttccgtttg ccttttaaat agtcctgggc ggcgtcggcc agggggctgt gccaataaaa
     2221 cgaccgctga t
//
LOCUS       DAOQLS010000338         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31696_length_2230_cov_3.642299, whole genome shotgun sequence.
ACCESSION   DAOQLS010000338 DAOQLS010000000
VERSION     DAOQLS010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31696_length_2230_cov_3.642299"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK362_06850"
     CDS             complement(<1..594)
                     /locus_tag="PK362_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNC74583.1"
                     /translation="MKLNQEQVLRYSRHLIMPEVGVEGQEKLVDAKVLLIGAGGLGTP
                     NALYLAAAGVGHMTLVDFDTVDHTNLHRQVIHGTGDVGKLKVESARETIRDINPNVNV
                     KTYNTPFTRDIALDLIGQHDIVVDGTDNFQTRYLTNDACVFLKKPNVYASIFRFDGQA
                     TVFKPGDPESPCYRCLYPEPPPPGEVPSCAEGGVLGIL"
     gene            complement(591..1247)
                     /locus_tag="PK362_06855"
     CDS             complement(591..1247)
                     /locus_tag="PK362_06855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC74584.1"
                     /translation="MTRRVLTADAVLFDLDGTLVDSRADIAFSVNHTLAALGRPTLDL
                     PTVERYVGDGVRQLLARSAGPMDEPTTARALEIFLPHYLEHCADTTRPYPGVMETLKR
                     LGGKALAVVTNKPEAHTWKTLRAAGMENLFQAVLGGDSLATRKPDPEPLWEALDRMKS
                     ARDRAVMVGDSLVDIRAARAARVPVVAVTYGFRPAEELSAAAPDALIQSFKDLTEVLS
                     "
     gene            complement(1244..1696)
                     /locus_tag="PK362_06860"
     CDS             complement(1244..1696)
                     /locus_tag="PK362_06860"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC74585.1"
                     /translation="MTLQVGDTAPDFTATTDGPQPLTLSTFLATNGKGKTVVLYFYPK
                     DDTPGCTVEACAFRDRQADFGQKNAVVLGVSLDGGESHRSFKTKFGLPFTLISDVDGN
                     LSRAYGVFNEAKGRSARWTFVIGPDRRIRKIFPNVAVEGHAAEVLSAL"
     gene            complement(1795..>2230)
                     /locus_tag="PK362_06865"
     CDS             complement(1795..>2230)
                     /locus_tag="PK362_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNC74586.1"
                     /translation="MEKVPVKVTYRLPPLSLLDELPLSYQRPQVEDKSVALLDSLASF
                     GVQASLLAIVQGPTVTRYELQPARGVKVSRFTSLTNDIAQVLMEKIKPLGAAVVIDAR
                     HMCMEMRGAESYSSPTVTSAMHGLFHKDARTREEFLSLIRQK"
BASE COUNT          397 a          700 c          713 g          420 t
ORIGIN      
        1 aaggatgccc aacacgcccc cttcggcgca ggagggcact tcgcccggcg gcgggggttc
       61 ggggtaaaga cagcggtagc aggggctctc ggggtccccc ggcttgaaca ccgtggcttg
      121 cccgtcaaag cggaagatgg aggcgtagac gttgggcttt ttcaagaaaa cgcaggcgtc
      181 gttcgtcaaa tagcgtgtct ggaaattgtc cgtgccgtcg accacgatgt cgtgctggcc
      241 gatcaagtcc agggcgatgt cccgggtgaa gggggtgttg tacgttttga cgttgacgtt
      301 ggggttgatg tcccgaatgg tttcccgggc gctctcgact ttgagttttc ccacgtcgcc
      361 cgtgccgtgg atcacctggc ggtgcaggtt ggtgtggtcc acggtgtcga aatccaccag
      421 ggtcatatgg cccacgccgg ccgccgccag gtaaagggcg ttgggcgtgc cgagcccgcc
      481 ggcgccgatc agcaatactt tggcgtccac gagtttctcc tgcccctcca cccccacttc
      541 gggcatgatg aggtggcgcg aataacgcaa gacttgctct tgattaagtt tcacgaaagg
      601 acctccgtca aatccttgaa cgattgaatc agggcgtccg gcgcggcggc ggacagttcc
      661 tccgccgggc ggaacccgta ggtcaccgcc accaccggca cccgggcggc ccgggccgcc
      721 cgaatgtcca ccaaactgtc ccccaccatc acggcccgat cccgagcgga tttcattcgg
      781 tccagggcct cccagagcgg ctccgggtcc ggtttgcggg tggccaaact gtcgccgccc
      841 aacacggctt gaaacaaatt ttccatcccg gcggcccgaa gcgttttcca ggtgtgggct
      901 tcgggcttgt tggtcaccac cgccagggcc tttccgccga ggcgtttcag cgtttccatc
      961 acgcccgggt agggccgggt cgtgtcggcg cagtgctcca aataatgcgg caaaaaaatt
     1021 tccagggcgc gcgcggtcgt cggttcgtcc atgggcccgg cggaccgggc cagcaattgg
     1081 cgaacgccgt ccccgacgta gcgctccacc gtgggaagat ccagcgtcgg gcggcccagg
     1141 gccgccaacg tgtgattaac ggaaaaggcg atgtccgccc gactgtcgac caacgttccg
     1201 tccagatcaa agaggacggc gtcggcggtc aaaacccgcc gcgtcacagg gccgacagga
     1261 cctcggcggc gtgcccttcc accgccacgt tcgggaatat ttttcgaatc cgccggtccg
     1321 ggccgatcac aaacgtccaa cgggccgagc gccccttcgc ttcattgaaa acgccgtacg
     1381 cccgggaaag gttcccgtcg acgtccgaga tcaacgtaaa gggcaaccca aattttgttt
     1441 taaacgaccg gtgggactct cccccgtcca agctgacgcc caggacgacg gcgttttttt
     1501 ggccgaaatc ggcttgccgg tcccgaaagg cgcaagcttc gacggtgcag ccgggcgtgt
     1561 cgtccttggg gtaaaagtaa aggacgaccg ttttcccttt tccgttcgtg gccaaaaagg
     1621 ttgaaagagt gagcggctgg ggcccatcgg tcgtcgcggt gaagtcgggc gccgtgtcgc
     1681 ccacttgaag cgtcatggag atctccttgg tcgttgcggc gtcgagcccg gcagccgtcc
     1741 cggccacggc aaaacacaac gcccaaagcc ctccgcgcgg gatggacacc gggcctactt
     1801 ttgccggatc agggaaagga attcttcccg cgtgcgggcg tctttgtgga acaggccgtg
     1861 catggcgctg gtcaccgtgg ggctcgaata actttcggcc ccgcgcattt ccatgcacat
     1921 gtggcgggcg tcgatgacga ccgccgcgcc caggggctta attttttcca tcagcacctg
     1981 ggcgatgtcg ttggtcaggc tggtgaagcg gctgactttg acgccccgag cgggctgcag
     2041 ttcatagcgg gtcacggtgg ggccctgcac gatagccagc aggctggcct gcaccccgaa
     2101 actggccagt gaatccagca gagccaccga cttgtcctcc acctgggggc gctggtagct
     2161 gagcggaagc tcgtccagca ggctgagcgg cggcagtcga taggtgacct tcaccggcac
     2221 cttctccatg
//
LOCUS       DAOQLS010000339         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31784_length_2226_cov_3.861815, whole genome shotgun sequence.
ACCESSION   DAOQLS010000339 DAOQLS010000000
VERSION     DAOQLS010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31784_length_2226_cov_3.861815"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PK362_06870"
     CDS             complement(<1..466)
                     /locus_tag="PK362_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC74587.1"
                     /translation="MDRTTLTPIPFMDLSPVHRELEAAATAVFRRVYEKNEFILGEEV
                     RLFEEEFARACGAAQGVGISSGTAALELSLRALGVGPGDEVITTPFSFFATASVVAYV
                     GAVPRFADVDPGTLNLDPTRLEKLYTPKTKAVLPVHLFGQPADMDAINAWAQS"
     gene            complement(448..921)
                     /locus_tag="PK362_06875"
     CDS             complement(448..921)
                     /locus_tag="PK362_06875"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HNC74588.1"
                     /translation="MKIPETFRRGLALGAPILRQFPGLVLLVSGYLKAVRPPEEFAAA
                     MESYWVLPVAVVLPLARVVPWLELLTGLALCTGYATRRAAFLAAGLHAVFIALLGQSL
                     VRGLTLKDCGCFGNLGPHLSPVQALALDAVLFLAALAVWTDRENRWSLDQWTERP"
     gene            complement(918..1535)
                     /locus_tag="PK362_06880"
     CDS             complement(918..1535)
                     /locus_tag="PK362_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC74589.1"
                     /translation="MNSGRRRAVGLALGAAALVAVALGRRTPFLADDVPAYRQKGPAG
                     APVVVTEYSDFQCPKCALVQPDLKNLLAQYEGRVRLEFRPYPLKSHRWSGDASRAAEA
                     AGLQGRYWEFADQLFAHQKEWADSPDPQPLFKDYAAAVGLDPARFEQDRTGDRVARIV
                     DAARAHAEALPVPATPTFFVNKRVLVGDLQLQAMGARFIERELEK"
     gene            complement(1542..1985)
                     /locus_tag="PK362_06885"
     CDS             complement(1542..1985)
                     /locus_tag="PK362_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74590.1"
                     /translation="MAKKAKSPSKAVKKKTAKPTARKPVAKKTPKKSAPAKKVVRRPA
                     PKAKAKKVEAPAPLPWRKALPGETFIGVVDDFYGHVSVIAFNLQGALAVGDKIHVRGH
                     TTDMTETVDSMQIDHNTVPSAKAGDAVGVRTIGKCRSGDYVYRVG"
BASE COUNT          403 a          699 c          741 g          383 t
ORIGIN      
        1 ggctttgggc ccaggcgttg atggcgtcca tgtcggcggg ttggccgaag aggtgcaccg
       61 gcaggacggc tttggttttg ggcgtgtata gcttttccaa tcgggtcggg tcgaggttca
      121 gcgtcccggg atccacgtcg gcgaaccggg gaacggcgcc gacgtaggcc accaccgagg
      181 ccgtggcgaa aaagctgaag ggtgtcgtga tcacctcgtc gcccggcccg acccccagag
      241 cccgcaggga caattccaag gccgcggtac ccgaggagat gcccaccccc tgggccgcgc
      301 cgcaggcccg ggcgaattcc tcctcaaaca gccggacttc ctccccgagg ataaactcgt
      361 ttttctcgta gacgcggcga aacaccgccg tcgccgccgc ctccagctcg cggtggacgg
      421 gggacaaatc catgaaggga atcggggtca aggtcgttcg gtccattggt ccagactcca
      481 tcggttttcc cggtcggtcc acaccgccag ggccgcgaga aacaaaacag cgtccagggc
      541 cagggcttgg acgggggaca ggtgcggccc gaggtttcca aaacagccgc agtccttcag
      601 ggtcaaaccg cggacgaggg attgccccag gagggcgatg aacaccgcgt gcaggccggc
      661 ggccaaaaac gccgcccggc gggtggcgta ccccgtgcac agggccaacc cggtcaggag
      721 ctccagccag gggacgacgc gggccagggg caataccacg gccaccggca acacccaata
      781 ggattccatg gcggcggcga attcctccgg cggtcggacg gctttaagat agccggagac
      841 gagcaacacc aggccgggga actgacgcaa aatcggcgcg ccgagggcca ggccccggcg
      901 gaacgtttcg gggattttca tttttccagc tcccgttcga tgaagcgggc gcccatggcc
      961 tgaagctgta ggtcgcccac caacacccgc ttgttgacga aaaaggtggg ggtcgcggga
     1021 acgggaaggg cttcggcgtg ggcccgggcc gcgtcgacga tccgcgccac ccggtcgccg
     1081 gtgcggtctt gttcaaagcg cgcggggtcc aatcccacgg ccgcggcgta atccttgaac
     1141 aggggctggg gatccggact gtcggcccac tctttttgat gggcgaaaag ctggtcggcg
     1201 aattcccagt agcgaccctg aaggccggcg gcctccgccg cccgggaagc gtcgcccgac
     1261 catcggtggc ttttcaaagg gtagggccgg aactcaagac ggacccggcc ctcgtactgg
     1321 gccaggaggt ttttaaggtc cggttgcacg agggcgcatt tggggcactg aaaatccgaa
     1381 tattccgtca ccaccacggg ggccccggcg ggccccttct gccgataggc cggaacgtcg
     1441 tcggcgagga agggcgtgcg acggcccagg gccacggcga ccagcgccgc cgcgcccaac
     1501 gccaacccca ccgcccgccg acgaccggaa ttcacgcggc ctcaccccac ccggtaaaca
     1561 taatcgcccg accggcattt gccgatggtg cgcacgccca ccgcgtctcc ggccttggcg
     1621 gagggcacgg tgttgtggtc gatctgcatg gaatccacgg tttcggtcat gtcggtggtg
     1681 tgcccacgga catggatttt atcacccacg gccagggcgc cctgaagatt gaacgcgatg
     1741 acgctcacgt gcccgtaaaa atcgtccacg accccgatga acgtctcccc ggggagcgcc
     1801 ttccgccagg gcaagggcgc cggcgcttcg acctttttgg ccttggcttt gggcgcgggg
     1861 cggcgtacga cctttttggc cggagcggat tttttgggcg tctttttggc gaccggtttt
     1921 cgggccgttg gctttgcggt ctttttcttc acggctttcg agggcgactt ggcttttttg
     1981 gccatgggcg gctcctttcg gacgatgggg atgcgcgggc aacgtcctga ttttagacaa
     2041 accggggccg atgcgcaaaa gaaacctgga cgtctttaca aaccggaaaa gcggggatag
     2101 gctaaaaggg cgattcctta ttcctttttc ggagggaagc ttgggcatta ttcagtggag
     2161 tccccgctac atcaccggac tggccctgat cgaccggcaa catcaggagc tctttcgccg
     2221 cctgaa
//
LOCUS       DAOQLS010000340         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_31788_length_2226_cov_3.632888, whole genome shotgun sequence.
ACCESSION   DAOQLS010000340 DAOQLS010000000
VERSION     DAOQLS010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31788_length_2226_cov_3.632888"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PK362_06890"
     CDS             complement(<1..575)
                     /locus_tag="PK362_06890"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HNC74591.1"
                     /translation="MATAPPSDIVAEIQRLKKIRNAVILAHNYERPEVQDIADVVGDS
                     LFLSHQAAKTSAEVIVFCGVHFMAETAAILSPNKTVLLPDLEAGCSLAATADVAQVRA
                     WKETHPGAVVVAYVNTTAAVKGEADYCCTSSNAVNVVRAIPADKEILFLPDMYLGQFV
                     REKTGRKIPLWPGSCHVHFGIHDADIEAQKKA"
     gene            complement(603..983)
                     /locus_tag="PK362_06895"
     CDS             complement(603..983)
                     /locus_tag="PK362_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74592.1"
                     /translation="MATFSAVLYSSTLDKFYEAVAFLAAARARGDTALLFLRGPALSA
                     FTQDRWDMPPAFASESVFHFDGKTPMEFLKDLHGHGKIRLYACSAWVRLLGLDNAAVA
                     ARVDAVIGLVAFLSQSQGGPVLSF"
     gene            complement(1011..1349)
                     /locus_tag="PK362_06900"
     CDS             complement(1011..1349)
                     /locus_tag="PK362_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74593.1"
                     /translation="MFVVTNSMFGLMILSLGILGIIISFLLTQRTRYFIALGLSTLLV
                     VIGLFFSVGTSLHQWRTARKIAKIQEQNRLTLEAIQSRIRQTPPAATEPRTTPVPAAR
                     PVETRRPARR"
     gene            1465..>2226
                     /locus_tag="PK362_06905"
     CDS             1465..>2226
                     /locus_tag="PK362_06905"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNC74594.1"
                     /translation="MKKISLRTKLSLYSSLLVLGVVSSVGFSLYVAERHYLLQRFQQS
                     QQESVQFLVQIGRESLTSRNMPLLASYLSLLRKSRALTYAMVLDAKGQIVAHTNTIFV
                     GQKPTDTVTLQALEAGTLTQQESPAGSDQVVDLAMPILQEGRRWGTARVGYSQKAIAA
                     LVDGALEAARHRIFVAGAVSIVVGIAIAVFLALLLARPIRHLRDGAHQIGEGALDHRI
                     RITSRDELGELANEFNVMAAKLQELDQLKQDFVSNV"
BASE COUNT          462 a          642 c          701 g          421 t
ORIGIN      
        1 gctttctttt gggcctcgat gtccgcgtcg tgaatgccga agtgaacgtg gcaagacccg
       61 ggccagaggg gaatcttccg ccccgttttt tcccgcacaa attggcccaa atacatgtcg
      121 ggcaaaaaga ggatctcctt gtccgccggg atggcccgaa cgacgttcac cgcgttggag
      181 gaagtgcagc aataatcggc ttcgcccttg acggcggcgg tcgtgttcac gtaagcgacc
      241 accacggccc cggggtgggt ttccttccag gcccgcacct gggccacgtc ggccgtagcg
      301 gccagggagc acccggcttc cagatccggg aggaggacgg ttttgttcgg ggaaaggatg
      361 gccgcggttt cggccatgaa gtgcacgccg caaaagacga tgacctcggc cgaggtcttg
      421 gccgcctggt gggacaaaaa caaggaatcc cccacgacgt cggcgatgtc ctggacctcc
      481 gggcgttcgt aattgtgggc caaaatcacg gcgtttcgaa ttttcttgag ccgttggatt
      541 tcggcgacga tgtcggaagg cggggcggtg gccatgacgg aattatatcc ctttgagggg
      601 cctcaaaacg acaggacggg gcccccttgg gattgggaca ggaacgccac cagcccgatg
      661 acggcgtcca cccgggcggc cacggcggcg ttgtcgagcc ccaagaggcg aacccaggcc
      721 gaacaggcgt aaagtcggat tttcccgtgc ccgtgaaggt ccttcagaaa ttccatgggg
      781 gttttcccgt cgaaatggaa aacggactcg gaggcaaagg ccgggggcat gtcccatcga
      841 tcctgcgtaa acgcgctcag ggccgggccc cggagaaaga gcaaggcggt gtcgccccgg
      901 gcccgggcgg cggccaggaa ggccacggcc tcgtagaatt tatcgagagt ggaggaatag
      961 agaacggccg aaaaggtggc cacggcgttc tccgtcgatt taagcgccga tcagcggcgc
     1021 gcggggcggc gggtttcgac gggacgggcg gccgggaccg gggtcgttcg gggttcggtc
     1081 gcggccggcg gggtctgtcg aatgcggctt tgaatggctt cgagggtcag ccggttttgt
     1141 tcctgaattt tggcgatttt acgcgccgta cgccattgat gcaggctggt gcccaccgag
     1201 aaaaaaaggc cgatcaccac gagaagggtg gagagtccca aagcgatgaa ataacgggtc
     1261 cgttgcgtca aaaggaagga aatgatgatt cccaaaatgc ccaggcttaa aatcatgagt
     1321 ccaaacatgc tgttggtcac aacgaacatg gacagcctcc ccaaatttta gttcgaaaat
     1381 aatcctaaca gataaacccc gcgccaacaa gggccaaatt tacgtccttg acggagggcg
     1441 gaccctctgt tacactggaa aaacttgaaa aaaatatccc tccgcaccaa attaagcctc
     1501 tattccagcc tgctggtgtt gggtgtggtt tccagcgtgg gattttccct ttacgtggcc
     1561 gagcgccact acttgttgca gcggtttcag caaagccaac aggaaagcgt ccagtttctc
     1621 gttcaaatcg gacgggaatc cctgacgagt cggaacatgc cgctcctggc cagttatttg
     1681 tcccttcttc gtaagtcccg ggccttgacc tacgcgatgg ttttagacgc taaagggcaa
     1741 atcgtggccc acaccaacac catttttgtc gggcaaaaac ccacggacac cgtcaccctg
     1801 caggccctgg aagcgggcac gttgacgcag caggaatccc ccgccggcag cgaccaggtg
     1861 gtggatttgg ccatgccgat tcttcaggag gggcggcgtt ggggcacggc ccgtgtcggg
     1921 tattcccaga aggccatcgc cgccctggtg gacggcgcgc tggaggcggc ccgccaccgg
     1981 atttttgtcg ccggcgcggt gtccatcgtc gtgggcatcg ccatcgcggt gttcctggcc
     2041 cttcttctgg cccggcccat ccgccatttg cgggacggcg cccaccaaat cggcgaaggg
     2101 gccctggacc accgcatccg catcacctcc cgggacgaat tgggcgagct ggccaacgag
     2161 ttcaacgtca tggccgccaa gctgcaggaa ttggaccaat tgaaacagga cttcgtctcc
     2221 aacgtc
//
LOCUS       DAOQLS010000341         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32064_length_2213_cov_4.465709, whole genome shotgun sequence.
ACCESSION   DAOQLS010000341 DAOQLS010000000
VERSION     DAOQLS010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32064_length_2213_cov_4.465709"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1612)
                     /locus_tag="PK362_06910"
     CDS             complement(122..1612)
                     /locus_tag="PK362_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNC74595.1"
                     /translation="MKRIKILGVLLLFPLTQWALSSSAQAAAPTTITYQGRILNNTGT
                     PVTTTKFITLTLKDLNSGSSDIVFAQNVTPSSQGEFSVSFSIPGSFKWDTAIARLEVK
                     VDSDVLTPTDQFGLTPYSIVAASVTAGGVTTNALANGAVTDVKVSTISASKIILPGGS
                     TPISTWQLGAGNFIDASKVALPGSTATVASWQYGSTGFIDVAKVTGTIPTTPAAHATS
                     HGLVGSDPLTNLSPQQISGTAVVSRSTSSQTIQPQVGSDVVPLVIKPNNSIPAASLDA
                     LAVYKADQSTTTLSVRGDGAITARGTLTVTGAVSANSGLSVTGNLSVSTITPSGNLNV
                     SGRVKDSYGFLVPAGTIVAFAGSSAPSGWLLCDGSTKDVSIQPELADLKAVLGSIYGS
                     ATATTFVLPNLKGRVPMGSGIGAGTTNWTIGVSTGEEKHQLTESEMPIHSHAGVVTAI
                     NYANVANGNSDSRATSHTVSSSQPKGGDQPHSIIQPSLVVNYIIKY"
     gene            complement(1609..>2213)
                     /locus_tag="PK362_06915"
     CDS             complement(1609..>2213)
                     /locus_tag="PK362_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74596.1"
                     /translation="DARVEIYAVLAEKKLALALWRQERQILLLKAENARKDGDLAAAK
                     ASYEQILNKDPGDADARTGLENLGSVVKAVEKAVVKEVRRKEVPDLLAKGTIDMVHER
                     YTDALESFHAVLQLEPGNAEAKEQINEVQRLMKGRKLYVPPVNVQSAADDKYREGLKL
                     YGNEQYSEAQAAFEEALKIDPHHDDARQALKRLKERETKP"
BASE COUNT          437 a          540 c          708 g          528 t
ORIGIN      
        1 tccgattaat cgttgattgg acggattgat cggccgcttt gaggggggaa atgagattaa
       61 ttacgacaag caataggggg gggaactttt tcattttgtg cggtcctttt ctaaggttca
      121 atcaatattt gattatgtaa ttgacgacca gggacggttg aatgatcgaa tgaggttgat
      181 ctccaccttt aggttgagat gaactcactg tatgagacgt tgctctgcta tcactgtttc
      241 cattcgcaac atttgcatag tttattgcgg tcactacacc ggcatgtgaa tgaattggca
      301 tttcggattc agttaattga tgcttctcct cgccagtgct tacgccaatg gtccaattcg
      361 ttgtccccgc gccaattcct gagcccatgg ggacgcggcc cttcaaattg ggcaaaacaa
      421 aagtggttgc cgttgccgat ccataaatag aaccgagcac tgccttcaaa tcagccaact
      481 ctggctgaat cgaaacgtct tttgtgctgc cgtcgcacaa caaccagccc gaaggcgcgg
      541 aggatccggc aaaagcgaca atggttcccg ccggaaccag gaaaccataa ctgtccttaa
      601 ctcggccact aacatttagg ttgcccgaag gggtgatcgt gctaacactc aaattaccgg
      661 tgactgaaag gccgctgtta gcactgaccg cgccggtgac cgtcaaagtc ccacgggccg
      721 tgatagcgcc atcgccgcga acggaaagag ttgtggtcga ctggtcggct ttgtaaaccg
      781 ccagggcgtc caaagaagcg gccgggatgc tgttgttggg tttaataacc aggggaacca
      841 catcgcttcc gacctgcggt tgaatggttt gggacgaggt ggaacgcgaa accacggcgg
      901 tgccggaaat ttgttggggg gacaggttgg tcaacgggtc ggagccgacc aggccgtggg
      961 aagtggcgtg ggcggcgggg gtcgtgggga tggtgccggt gactttggcg acgtcgatga
     1021 agccggtgga gccgtattgc caggaggcca ccgtggcggt gctgccggga agggcgactt
     1081 tggaggcgtc gatgaaattg cccgcgccca actgccaggt ggaaatgggg gtggagccgc
     1141 cgggaaggat gattttggag gccgaaatag ttgaaacttt cacgtcggtg acggcgccgt
     1201 tggccagggc gttggtggtg acgcccccgg cggtgacgct ggcggccacg atgctatagg
     1261 gggtgaggcc gaattggtcc gtgggggtca atacgtcgga atccactttg acttcgagtc
     1321 gtgcgatggc ggtgtcccac ttgaaactgc cggggatcga aaaggaaacg ctgaattcgc
     1381 cctgggagga gggcgtgacg ttctgggcaa agacgatgtc gctgctgccg gaatttagat
     1441 ccttcaacgt cagcgtgatg aatttggttg tcgtgacggg cgttccggtg ttgttcaaaa
     1501 tgcgcccctg ataagtgatg gtggtggggg ccgccgcctg ggccgacgag gaaagagccc
     1561 attgggtcag gggaaataga agtaaaaccc cgaggatttt aattcgtttc atggtttcgt
     1621 ctcccgctct ttaagacgtt tgagcgcctg tcgggcatcg tcgtggtggg ggtcgatttt
     1681 gagggcttct tcaaaagcgg cctgggcttc ggaatattgt tcgttgccgt aaagtttaag
     1741 gccctcccgg tatttatcgt cggcggcgct ttggacgttg acgggaggga cgtagagttt
     1801 gcgccctttc atgagccgct gcacttcgtt gatctgttcc ttggcttcgg cgttgccggg
     1861 ttccaactgc aagacggcgt gaaaggattc cagggcgtcg gtgtatcgct cgtggaccat
     1921 gtcgatggtg cccttggcca ggaggtcggg gacttctttg cggcgcactt ctttaaccac
     1981 ggctttttcg acggctttga cgacggaccc cagattttcc aaacccgtcc gggcgtcggc
     2041 gtcgccggga tctttgttca atatttgttc gtaggaggcc ttggccgcgg ccaggtcgcc
     2101 gtctttcctg gcgttctcgg ctttaaggag aaggatttgc cgctcctgcc gccagagggc
     2161 cagggccagc tttttctccg ccagcacggc gtaaatttcc acccgggcgt ccg
//
LOCUS       DAOQLS010000342         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32068_length_2213_cov_3.759963, whole genome shotgun sequence.
ACCESSION   DAOQLS010000342 DAOQLS010000000
VERSION     DAOQLS010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32068_length_2213_cov_3.759963"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2213
                     /locus_tag="PK362_06920"
     CDS             <1..>2213
                     /locus_tag="PK362_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74597.1"
                     /translation="NYAILNGRAVVAKSTTQSTTTAGADTTVNVEETTYGYNENGQLS
                     SASGRTISSRTESRVKDAHGSEKIVVTVNDPAHVSTTVFAVVWGQAVAVRTDSWQITY
                     SGYDGSDLAHSAVETHTHSVTENQYNSLGQLIHTTGRSDGFTITRYADSTPDRSVARE
                     SRQDFSSENVYMIRAGQALIVDSVTNSRLVMGATTTTSTSNTHYNYNSRNQLISASGG
                     STSDTTQENSDTSLQISHSDTTSTYVVRYGKAVVSDSVTTSNSRVDTRTAADRAAGVP
                     EAGNDINVTTSTSEIHFAVNELGQVTGATGSSESETTSRVFTDKNRNGKIDAGEEIDV
                     PSSSSTTNVYDMRWGQAVLIHTETTSSNALAATDSSTTSKIDYRYNSKGQLVSASGTT
                     DTTTNTRNADGTVTRTPSRATNVYAIVFGQAQIARTTVHSSTSAGASDTGASNVTEYF
                     YNEKGQLVSARGSGSSSTTTYSADYSGAINRVNADKTREHMTERVSPDGTVTLNYEKF
                     DETGKRIVWGTIVERTDSAGKVTVQTNGKYIDGKNADGSDKVVDVNVNSNPIQSTTDA
                     LKKGITLTNASTSTSHSNTTYKIIAGQAVAIQTTTSTTSTNLGTVSHNVTTVINTYNQ
                     RAQLIAATGRVTGSSTTAYWNDLNGDGVWQEDTETEEQNAVSEGSIVYLILNGQAAQS
                     DSHMVTQTEIGATKSVTTADTHYSYNQLGQLTGGYGTSHTESVTTYLDESEQKGV"
BASE COUNT          552 a          757 c          578 g          326 t
ORIGIN      
        1 acaattacgc cattttgaac ggtcgcgccg tggtggccaa atccaccacc caatcaacca
       61 caaccgccgg cgccgatacc acggtgaacg tggaagagac gacgtatggg tacaacgaaa
      121 acggccaact ttcgagcgcc agcgggcgga cgatctcgtc ccggaccgag tcgcgggtca
      181 aagacgccca tggatcggaa aagattgtgg tgaccgtcaa cgatccggcc cacgtcagca
      241 ccaccgtgtt cgccgtggtt tgggggcagg ccgtcgccgt ccgcacggac agctggcaaa
      301 tcacctattc cggttacgac ggatcggatc ttgcacactc cgccgttgaa acgcacaccc
      361 attccgtcac cgagaaccag tacaacagcc tgggccaatt gattcacacg accggccgca
      421 gcgatggatt taccatcacc cgatacgccg acagcacccc ggatcgctcc gtggcccggg
      481 aatcccgcca ggacttttcg tccgaaaacg tttacatgat tcgcgccgga caggctttga
      541 tcgtggacag cgtgaccaac agccgcctcg tcatgggggc gacaacgacg acctccacga
      601 gcaacacgca ttacaactac aattcccgaa atcaattaat ttccgcgtcg ggcggctcca
      661 cgtccgacac gacacaagaa aacagcgaca cttcgcttca gatctcccac agcgacacca
      721 cctccaccta cgtggtgcgt tacggcaagg ccgtcgtgag cgattccgtc accacgtcca
      781 attcgcgcgt cgacacccgc acggcggccg accgcgccgc gggcgtcccg gaggcgggca
      841 acgacatcaa cgtcaccacg tcgacttccg aaatccactt cgccgtgaac gaattgggtc
      901 aggtgacggg ggccaccggg tcgagcgaaa gcgaaaccac ttcccgcgtt ttcacggaca
      961 agaaccgcaa cggcaaaatc gacgccggcg aggaaatcga cgtgcccagc agctcctcca
     1021 cgaccaacgt gtatgacatg cgctggggcc aggcggtgct gatccacacc gaaacgacgt
     1081 caagcaacgc cttggcggcc accgattcct ccaccacgag caaaatcgac taccgataca
     1141 attccaaagg ccaattggtt tccgcgagcg gcaccacgga cacgacgacc aacacccgca
     1201 acgccgacgg aacggtgacc cgaaccccca gccgggccac aaacgtttac gccatcgtgt
     1261 tcggccaggc ccaaatcgcc cgaaccaccg tccactcttc cacctccgcc ggcgccagcg
     1321 acaccggggc ttccaacgtc accgaatatt tctacaacga gaaagggcag ttggtgagcg
     1381 cgcgggggtc cggatcgtcc tccacgacca cctacagcgc cgactacagc ggcgcgatca
     1441 atcgcgtgaa cgccgacaag acccgcgaac acatgacgga acgggtctcg cccgacggca
     1501 cggtgacgtt gaactacgag aaattcgacg aaaccggaaa gcgcatcgtt tggggcacca
     1561 tcgtcgagcg gaccgattcc gccggcaaag tcacggttca aaccaacggc aaatacatcg
     1621 acggcaagaa cgcggacggg tcggacaagg tcgtcgacgt caacgtcaat tcgaacccca
     1681 ttcagtccac gacggacgcg ttgaagaagg gcattaccct caccaacgcc agcacctcga
     1741 cctcccactc caacaccacg tacaaaatca tcgccgggca ggccgtggcc attcaaacga
     1801 cgacgtccac cacttccacg aatctgggga ccgtttccca caacgtcacc acggtcatca
     1861 acacctacaa ccaacgcgcc caattgatcg ccgccaccgg acgggtgacg gggtcctcca
     1921 ccacggccta ttggaacgac ctcaacgggg acggcgtctg gcaagaggac accgaaaccg
     1981 aggagcaaaa cgccgtttcc gaagggtcga tcgtctatct catattgaac ggacaggccg
     2041 cccagtccga ttcccacatg gtgacgcaaa ccgagatcgg cgcgaccaaa tcggttacga
     2101 cggccgacac ccattattcc tacaaccaac tgggtcaatt gaccggcggt tacgggacat
     2161 cccacaccga atcggtgacc acctacctgg acgaatcgga acaaaagggc gtt
//
LOCUS       DAOQLS010000343         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32071_length_2213_cov_3.561631, whole genome shotgun sequence.
ACCESSION   DAOQLS010000343 DAOQLS010000000
VERSION     DAOQLS010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32071_length_2213_cov_3.561631"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PK362_06925"
     CDS             complement(<1..1017)
                     /locus_tag="PK362_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC74598.1"
                     /translation="MPSRPAFPRVSWTELLRDFFPLQQRPLEVLLRWQRRFGPTFWLP
                     GALPRLVLSAPEDVRHVLVARQDNYWKVGNFALLRDVLGEGILTSNGPLHDRQRRAWM
                     PHFQKNVLSEVGPRASRHTDELTRAWKPGDAVELGGLFARVAVALLGDVLLGVDLRGE
                     AEKIFRSGLAIQTYVGTGVFTPWPGFFRRFRARSFARARDAVVGVAEGLLAGWKPPAG
                     PPTAGLARTVLAAGEPGDPEAERRRRDDIVTLLLTGHETTANALSWTVALILNTPDVR
                     AAVEGEARSAAFRWEDSLLRRCFQEALRLYPPAWCIGRRAMADDVLPGGAFLPAGGHA
                     VVFSA"
     gene            1097..>2213
                     /locus_tag="PK362_06930"
     CDS             1097..>2213
                     /locus_tag="PK362_06930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC74599.1"
                     /translation="MPGRRFLMVYPTFRPSVAGVQYALPLFGKKSLLPPLGLLTVAAL
                     TPPEYEIRLIDMNCRPLTEEDLRWADAVLFSAMVTQREALFAAAAQARALGKFVVFGG
                     PFPTSCADDCRPHCDALVLNEGEITWPLFLADWEKGRPAALYRTEDKADVRRSPPPRF
                     DLVRLSDYGAGNVQFSRGCPFQCEFCDITTLLGRVPRLKTADQFLAELDRLHAAGHRG
                     QVWVVDDNFIGNFRDLQALLPRLKEWNVAHGHPFHYFFEASSNLADHPEMLRALVEAR
                     FNLVFVGLESPAMESLTETRKFQNLRGSLVDKVRVLQRAGLAVMGGFVVGFDNDPPDI
                     FERQQRFIEATAVADAYISVLVALPGTPLFDRLRREGR"
BASE COUNT          395 a          755 c          689 g          374 t
ORIGIN      
        1 ggccgaaaag accacggcgt gcccccccgc cggcaagaac gcgccgccgg gcagaacgtc
       61 gtccgccatc gcccggcggc cgatgcacca ggccgggggg tagaggcgga gggcctcttg
      121 aaagcaccgt cgtagaaggg aatcctccca acggaaggcc gccgatcgag cctccccttc
      181 caccgccgcc cgaacatcgg gcgtgtttaa aatcaacgcc acggtccagg aaagggcgtt
      241 ggcggtggtt tcgtggcccg tcaaaaggag cgtcacgatg tcgtcccggc gtcggcgttc
      301 cgcctccggg tcccccggtt cgcccgcggc caacaccgtc cgggccaacc ccgccgtggg
      361 gggaccggcg ggaggtttcc atccggccag aagcccctcg gccacgccga ccaccgcgtc
      421 ccgggcccgg gcgaatgacc gcgccctgaa ccgacgaaag aagcccggcc agggcgtgaa
      481 cacgcccgtt ccgacgtagg tttggatcgc caaccccgac cgaaaaattt tctccgcctc
      541 cccgcgcaaa tcgacgccaa gcaggacgtc ccccaacagc gccaccgcca cccgggcgaa
      601 aaggccgccc aattccaccg cgtcgcccgg cttccaggcg cgggtgagct cgtccgtgtg
      661 gcgcgaggcc cggggaccca cctccgacaa aacgttcttt tggaaatggg gcatccaggc
      721 gcgacgctga cgatcgtgga gggggccgtt gctcgtcaag atgccttccc ccagcacatc
      781 gcggaggagg gcgaaattgc cgactttcca ataattgtcc tgccgggcca ccaacacgtg
      841 ccggacgtcc tcgggggccg aaaggaccag ccgcggcaac gcgcccggca gccaaaaggt
      901 cggtccgaag cgccgttgcc aacgcagcaa cacctccaag ggacgctgtt gaagggggaa
      961 aaaatcccgg agcaattccg tccacgaaac gcggggaaag gcggggcggg aaggcatgga
     1021 ggaatcctag aattcaatcg ggggccggtc aacggccggg cttgtcgggg gggccgttcg
     1081 ggtgtacact caagggatgc ccggtcgtcg gttcctcatg gtgtatccca ccttccgccc
     1141 ttcggtggcc ggggtccagt acgccctgcc cctgttcgga aaaaagtcct tgttgccgcc
     1201 cctgggcctg ctcaccgtcg cggccctcac gccgccggag tacgaaatcc gcttgattga
     1261 tatgaattgc cggcccctga ccgaggagga tttgcgttgg gccgacgcgg tgcttttttc
     1321 ggccatggtc acccagcggg aggccctctt cgccgccgcc gcccaggccc gggcgctggg
     1381 gaaattcgtg gtgttcggcg gacccttccc cacctcctgc gccgacgact gccggcccca
     1441 ttgcgacgct ctggtactta acgaggggga aatcacctgg ccgctctttt tggccgattg
     1501 ggaaaaaggc cgacccgccg ccctgtatcg aacggaggac aaggccgacg ttcgccggag
     1561 ccctccgccc cgtttcgact tggttcgcct gtcggattac ggggccggca acgttcaatt
     1621 ttcccgggga tgcccctttc aatgcgaatt ttgcgacatc acgaccctgc tcggccgggt
     1681 gccccgccta aaaaccgcgg atcaattcct ggcggaactg gaccgccttc acgccgccgg
     1741 ccatcggggt caagtatggg tggtggacga caatttcatc ggaaatttcc gggacctcca
     1801 ggccctgttg ccccgtttga aggaatggaa cgtcgcccac ggccacccgt ttcattactt
     1861 tttcgaggcg tcctccaatc tggcagacca cccggaaatg ctccgcgcgt tggtggaagc
     1921 ccggttcaat ttggtgttcg tggggctgga atctcccgcc atggaatccc tgaccgaaac
     1981 gcggaaattt caaaacctcc gggggtctct cgtggacaag gtgcgcgttc tccaacgggc
     2041 cggtttggcg gtcatgggcg gctttgtcgt cggttttgac aacgacccgc cggacatctt
     2101 cgagcgccaa caacgattca tcgaagccac cgccgtggcc gacgcttaca tcagcgtctt
     2161 ggtcgccctg cccggaaccc cgcttttcga ccgacttcgc cgggaaggcc gcc
//
LOCUS       DAOQLS010000344         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32107_length_2212_cov_2.924432, whole genome shotgun sequence.
ACCESSION   DAOQLS010000344 DAOQLS010000000
VERSION     DAOQLS010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32107_length_2212_cov_2.924432"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2212)
                     /locus_tag="PK362_06935"
     CDS             complement(<1..>2212)
                     /locus_tag="PK362_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74600.1"
                     /translation="RGRVEARWADGRWEARAAACGGTIAVQSVSESTGVSPSSWTAVA
                     ENLDLGVWARENGWPEVGGRVGGQFVFARRPAEEVGGWPVRGDGAWGVENFRWGAHHR
                     AETVSGHLALREDGFRATGDGGRFVAAVDVSTEGWRIDRLAYAAPEGFSFEGSGRLTG
                     RPAVWDLRARLEGFPLRDVPPVLKHFPDVAGALSADGALTGPLNEPRFEGGVRLQGVR
                     WRPGGALHAADMEFHGSRRYVRLPRLAWDDTLTADGAWVPGRGWQLSATFNKTDASRA
                     FDFWAGAVDLAGTVEGQISLSGAPGAPLEETRGWGNLRWNDGRWGSVEFQRATGVFFL
                     EKAKIVLDGLDLHQSSGDFHADGEWNRESPTRWSWRTNLRAAGFQSGGGWDGTAAAAG
                     AFERGRWTGTVVSPGLSWGGIGLGEIRAGFQADPSGLSVNPFEAGAGLRGEFRWERSD
                     GRVDGWATLDNARWADLLPLRVRSPWLHRADIVPGRGRAAVRLSGTGGKPVIEFSGSV
                     AGAQWKTVGVEVEAAGVWSDHRWVISTATARLGSGGAATAHGEGTTSTSSAPAVAVWS
                     VDFNDVGLGPVLTALAPDNHWEGRAAGRLEGRGPLADPEIELRAGGTIEREETIEWAI
                     AADCVRRGWTIRRADFLTPEGSLKIRPGGTVAPETPGILRAQFVADARNIHAGPLTLF
                     GALGVDGRWRGDPRDVSARLTARSLWVNQQWLDRDLAQIHWTPGQLDFEPLSGGA"
BASE COUNT          376 a          878 c          661 g          297 t
ORIGIN      
        1 ggcccccccg gacaagggct caaaatccag ttgtcccggc gtccaatgga tttgggcgag
       61 gtctcgatcc aaccattgct gattgaccca cagggaacgg gcggtcaaac gggccgaaac
      121 gtcccgggga tccccccgcc accgaccgtc cacccccaag gcgccgaaca gagtcagggg
      181 acccgcgtgg atgttgcgcg cgtcggccac gaattgggcc cggaggattc ccggcgtctc
      241 gggggcgacc gttccgccgg gtcggatctt gagggaccct tcgggcgtga gaaaatccgc
      301 ccgacggatc gtccaaccgc ggcggacgca atccgcggcg atcgcccatt cgatggtctc
      361 ttcgcgctcg atcgttccac cggcccgaag ttcgatttcc gggtccgcca ggggtccgcg
      421 cccctccagg cggccggcgg cccgtccctc ccaatgattg tccggagcca gcgcggtcaa
      481 aacggggccg agccccacgt cgttaaagtc caccgaccag accgccaccg cgggggccga
      541 cgacgtggac gtggtcccct cgccgtgggc cgtcgccgcc ccgccggacc cgagccgcgc
      601 ggtcgccgtt gaaatcaccc atcggtggtc ggaccacacc ccggccgcct ccacctccac
      661 gcccaccgtc ttccattggg cccccgccac cgacccgcta aattcaatca cgggtttccc
      721 gccggttccg gaaagacgaa ccgccgcgcg tccccgcccg ggaacgatgt cggcccgatg
      781 caaccagggc gagcgaacgc gaagggggag caagtccgcc cagcgggcgt tgtccaacgt
      841 cgcccacccg tccactcgtc cgtcgctccg ttcccaacga aattcgcccc gaaggcccgc
      901 tcccgcctcg aaggggttca ccgacagacc ggacgggtcg gcttgaaaac cggcccggat
      961 ttcccccaac ccgatgccgc cccaggacaa cccgggcgag accaccgttc ccgtccatcg
     1021 gccccgctcg aaggcccccg cggcggcggc ggttccgtcc cacccccccc cggattggaa
     1081 ccccgcggcg cggagattgg tccgccagct ccaacgggtc ggggattccc ggttccattc
     1141 gccgtccgcg tgaaaatcgc ccgacgattg atggaggtcc aacccgtcca aaacgatttt
     1201 ggccttttcg aggaaaaaaa cgcccgtggc tcgctgaaac tcgacggacc cccaccggcc
     1261 gtcgttccac cggagatttc cccagccgcg ggtctcctcg aggggcgcgc ccggcgcccc
     1321 ggaaagggag atctgcccct ccaccgttcc ggccaggtcg accgccccgg cccagaaatc
     1381 aaaggcccgc gacgcgtccg tcttgttgaa agtcgccgac agttgccatc cccgccccgg
     1441 gacccaggcg ccgtcggcgg ttagcgtatc gtcccaggcc aaacgcggca atcggacgta
     1501 ccggcgactg ccgtggaatt ccatgtcggc ggcgtgaagc gcgccgcccg gacgccagcg
     1561 aaccccttga agccgcaccc cgccctcgaa ccggggttcg ttcagtggac cggtcagcgc
     1621 cccgtcggcc gacaacgccc ccgcgacgtc gggaaaatgt ttgagtacgg ggggcacgtc
     1681 ccggagcgga aacccctcga gacgcgcccg cagatcccag accgccggac ggccggtcaa
     1741 ccgcccactt ccttcgaagg aaaaaccctc gggcgccgcg tacgccagtc ggtcgatgcg
     1801 ccacccttcc gtggacacgt cgaccgcggc cacgaaacgc ccgccgtccc ccgtcgcccg
     1861 aaatccatct tcccgcaggg ccagatggcc ggaaacggtt tcggcccggt ggtgggcccc
     1921 ccagcgaaag ttctcaacgc cccaggcccc gtccccccgc accggccacc cgccgacctc
     1981 ctccgcgggg cgccgggcaa aaacaaattg tccgccgacc cgaccaccga cttcgggcca
     2041 cccgttctcc cgagcccaaa cacccaaatc cagattttcg gccacggcgg tccacgacga
     2101 aggggacacc cccgtggatt ccgacacgct ttgaacggcg atcgttccgc cgcaggccgc
     2161 cgcgcgcgct tcccaccgac cgtccgccca gcgggcctcg acgcgacccc gt
//
LOCUS       DAOQLS010000345         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32273_length_2206_cov_4.231055, whole genome shotgun sequence.
ACCESSION   DAOQLS010000345 DAOQLS010000000
VERSION     DAOQLS010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32273_length_2206_cov_4.231055"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2206
                     /locus_tag="PK362_06940"
     CDS             <1..>2206
                     /locus_tag="PK362_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC74601.1"
                     /translation="LEMTFGLPAGVSLLLAAGTAVALFPLGHIGGVHLFEPGGRLRLT
                     ETTAQDLTKLTILGAGLRVAHLGLVLGLPWMFTALAGAPILGPPGALIELAAQLLPFL
                     AVPLLHGRYNANPPRGWPLGILSFRHALLAIRWGTTVSAGFFERFVPRPGVPVGDGYI
                     SNPRRTALIDRVVGALAATSDARLNNLHPLALRGILDIVLKNVEQHGVPGAEAQAAGR
                     IDGDDLLLEVRNTSDVPLAPELNGAFSLDNINGRVAAENRSPGTGYGFGVTRIPDLAR
                     GLYLPEFESFAGRARWSWSTDRDTRDVVFQLRVPLPPAPAAAKPHRFVSEWEMPGEAS
                     PASPAPGVWSAIGRRFVAVTAAVALFASGLALDSSRAWARPAPSIPGATASDGRNDRL
                     PAVLATGAMVVPLVAASRRRRSDFWNQVGDGKHPGWRLVHVPGDNGETIEAAVKDAGR
                     GPVVVWNYGGGSGPVLERQADWFADRPAVVVRRREAPADALVGQTAGDTIRALQTLRF
                     QGGLDWTARGALIVGHSLGAHVATNVALRLHSLSRGAVRAVVLLNPPVPNGLWYSTLI
                     GWLSFLKGASETLDSWLEAARFSSASRRSLRQQESQWWGHFIETWIPSMDDARSDIEA
                     PPAIPWALFNGWDHMLSESRALGHYLETTLTDRLEELSKLPAPVLVVASPHDPLAGEI
                     HKLQKALALEAIPDANLPAALSASPRARVFVEINNGGADVLVEHSPHMSALAP"
BASE COUNT          298 a          770 c          720 g          418 t
ORIGIN      
        1 gctggaaatg accttcggtc tccccgccgg ggtgagcttg ctcttggcgg ctggaacggc
       61 cgtggccctt ttccctctgg gacacatcgg gggcgttcat ttgtttgaac cggggggccg
      121 cctacgcctc accgaaacca ccgcccagga tttgaccaag ttgacgatct tgggcgcggg
      181 attgcgcgtc gcccacctcg gcctggtgtt gggtctgccg tggatgttca cggccctggc
      241 cggcgccccg atcctggggc ccccgggcgc gttgatcgaa ttggccgcgc aactcctgcc
      301 ctttttagcg gtgcccctcc tccacgggcg ctacaacgcc aacccgcccc ggggctggcc
      361 cctgggcatc ctctccttta gacacgccct gctggcgatt cgttggggca ccacggtgtc
      421 cgccggattt tttgaacgct tcgttccccg ccccggcgtt cccgtgggcg acggatacat
      481 atccaacccc cgccgcacgg ccctcatcga ccgggtcgtg ggcgccctgg ccgccacgtc
      541 ggacgctcga ttgaacaacc tccaccccct ggcgcttcgg ggcatcctcg acatcgtttt
      601 aaagaacgtg gaacagcacg gcgtgccggg ggccgaggct caggccgcgg ggcggatcga
      661 tggggacgac ctgcttttgg aagtccgcaa cacctccgac gtcccgttgg cgccggaatt
      721 gaacggggcg ttttcgctcg acaacatcaa cggccgcgtg gccgccgaaa accgatcccc
      781 cggcacgggg tatggattcg gcgtcacccg gatccccgat ctggcccgtg ggttgtacct
      841 tcccgaattc gaatccttcg ccgggcgggc ccgctggtcc tggtccaccg accgggacac
      901 ccgggacgtc gtgtttcaat tgcgggttcc gctgccgccc gcgcccgcgg cggcgaagcc
      961 gcatcgattt gtttccgagt gggaaatgcc gggcgaagcg tcgccggcgt ccccggcccc
     1021 cggggtgtgg tccgcgatcg gccgacggtt cgtcgccgtg acggcggccg tggctctctt
     1081 tgcgagcggc ctggccctgg attcctcccg ggcctgggcc cgtcccgcgc cgtcgatccc
     1141 gggggcgacc gcttccgatg gacgcaacga tcgtcttccc gcggtgttgg cgacgggcgc
     1201 gatggtcgtt cctctggtcg cggccagccg tcggcgccgc tccgattttt ggaaccaagt
     1261 gggggacggt aagcatcccg ggtggcgctt ggtccacgtc ccgggcgaca acggggaaac
     1321 catcgaggcc gccgtgaaag acgcggggcg cggccccgtc gtcgtgtgga attacggcgg
     1381 cggttcgggg cccgtcctgg aacgccaggc ggattggttc gccgatcggc cggcggtggt
     1441 ggtccgccgt cgggaagccc cggcggacgc ccttgtgggt caaaccgcgg gcgatacgat
     1501 ccgggccctg cagacccttc ggttccaagg cgggttggat tggacggccc ggggcgcgtt
     1561 gatcgtcggt cattccctgg gggcccacgt ggccaccaac gtggcgctcc gcctgcattc
     1621 cctttcccgc ggagccgtgc gggcggtggt tctcctcaac ccccccgtgc ccaacgggct
     1681 gtggtattcg acgttgatcg gttggctttc gttcctgaaa ggcgccagcg aaaccctgga
     1741 ttcctggttg gaggccgcgc gattttcctc cgcttcccgt cgatcgcttc ggcagcaaga
     1801 gtcccaatgg tgggggcatt tcatcgaaac gtggattccc tccatggacg acgcccggtc
     1861 cgacatcgaa gccccgccgg ccattccctg ggcgctgttc aacggctggg accacatgtt
     1921 gtcggaatcc cgagccctgg gtcattattt ggaaacgacc ctgacggacc ggttggagga
     1981 gctttccaaa cttcccgccc cggtgctcgt cgtcgcctcc ccgcacgacc ccctggccgg
     2041 cgaaatccat aaacttcaaa aggccctcgc cttggaagca atccccgacg cgaatctccc
     2101 cgccgccctc tccgcatccc cccgggcgcg ggtttttgtc gaaatcaaca acgggggcgc
     2161 ggacgtgttg gtcgagcatt cgccccacat gtcggcgttg gccccc
//
LOCUS       DAOQLS010000346         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32320_length_2204_cov_3.910191, whole genome shotgun sequence.
ACCESSION   DAOQLS010000346 DAOQLS010000000
VERSION     DAOQLS010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32320_length_2204_cov_3.910191"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK362_06945"
     CDS             complement(<1..267)
                     /locus_tag="PK362_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC74602.1"
                     /translation="MSIEVRGLTKRFGAFKAVDDVSFRLESGSLVALLGPSGSGKSTL
                     LRMIAGLERPDAGDILLTGEEATTQSARQRNVGFVFQHYALFKHM"
     gene            complement(589..>2204)
                     /locus_tag="PK362_06950"
     CDS             complement(589..>2204)
                     /locus_tag="PK362_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74603.1"
                     /translation="GYVAARTDRLATLTSADLDGFEASLPAEIRANPVFGVRPTMFVV
                     TVDERILRNDPSLPESPYFLTELLTKAASELLVRHQAQGGPDLWLRALRSIADVEVLE
                     RKDGVDAGAPRLIRVTFNPAKIGPLMSHFRRAQETASRRAHKELTGEAMPLVAVTGSE
                     GLLDNWARSGRANPEDPAKALDRMFPVLRPLGIEASLRDRSAAVRLEAGVRSGYARWW
                     ARQMTENISGVDAPATIEQAARWGQRELPVGDFARVAAEAFDKGEGAALLLDAAQRLQ
                     DLPDVRENDDTFGAWLEATADRLVLSWFARRADLRDAGRVHGVKWLVAAAGLAATGPR
                     GFDAPRFEKLARRLESRLNSDLREIFRLESERTRFQAAFAAGRDVVIDLAGAASREKQ
                     LLATLDRLGRARRENPASGSRVVFVDRGAASDAVLEKDARALAARAGWTLSDASAFRF
                     FANEDAHRALFADGRTHLRELALRLQSHPGESLRPMDVFTPSAEGWDLSGVETLVQLL
                     ISLAGDLVYNATERLGRDLQHAKFVSINA"
BASE COUNT          381 a          726 c          746 g          351 t
ORIGIN      
        1 catgtgttta aaaagggcgt agtgctggaa aacgaagccg acgtttcgtt gccgggcgga
       61 ttgggtggtg gcttcctcgc cggtgaggag aatgtcgcca gcgtccggac gctccaaccc
      121 ggcgatcatt cgaaggaggg tgcttttgcc agagcccgac ggccccaaca gggcgaccag
      181 ggagcccgat tcgagacgga aggacacgtc gtccaccgcc ttgaaggcgc cgaaccgttt
      241 ggtcaatccc cgcacttcaa tgctcatggg tctccgttcc tcgcaaaaaa aatcccgtgc
      301 gcgaaccggc ggcacgggag gtccagccgc tttagcgccc tgtctgtccc gtccgtccgc
      361 ggtcgtcgcg gtcgaaccgc tccccggatc gacccgggga gtcactttcc agtgaaaacc
      421 ggccggggca agttggtgct taaagcctcg ccgttaggcg gggtccccgt tttcccgcgg
      481 ccacgcgggt tggggacctc gccgcacaac ggatcgccgc cttcttcgga gcgccgcgtc
      541 gtgaaatcga ttataggata gttgattccg ggaaacaacg gcccggcgtt aggcgttgat
      601 ggagacgaat ttcgcgtgtt ggagatcccg gcccagacgc tcggtggcgt tgtagaccag
      661 gtccccggcc agggaaatca gcaattggac cagcgtttcc acaccggaca agtcccagcc
      721 ttcggccgag ggggtgaaca cgtccatggg gcgcagggat tcccccggat gggattgcag
      781 gcgaagagcc agttcccgaa ggtgcgtccg gccgtcggca aagagggcgc ggtgggcgtc
      841 ctcgttggcg aaaaagcgga aggccgacgc gtccgacagg gtccacccgg cccgggccgc
      901 cagggcgcgg gcgtcttttt ccaggacggc gtcggacgcc gcgccgcggt ccacgaagac
      961 cacgcgggag ccggaggccg gattttcccg acgggccctt cccagacggt ccaaggtggc
     1021 caatagctgt ttttcccggg acgccgcccc cgccaggtcg atcacgacgt cccggccggc
     1081 cgcgaacgcc gcctggaacc gggtgcgttc ggattccaac cggaatattt cccgaaggtc
     1141 gctgttcagc cgggactcca agcgccgcgc caatttttcg aaccggggcg cgtcgaaccc
     1201 ccggggcccc gtggcggcga gaccggcggc ggccaccagc catttgacgc cgtgaacccg
     1261 tcccgcgtcc cgcaggtccg cccgacgggc gaaccaggaa aggaccaggc ggtccgccgt
     1321 ggcctccaac cacgccccga aggtgtcgtc gttttcccga acgtccggga ggtcctgcaa
     1381 ccgctgggcc gcgtccaaaa ggagcgcggc gccttctccc ttgtcaaacg cttcggccgc
     1441 gacccgggcg aaatccccga ccggcagttc ccgttggccc caccgggcgg cttgctcgat
     1501 ggtcgcgggg gcgtccacgc ccgatatgtt ttcggtcatt tgtcgggccc accagcgggc
     1561 gtagccgctt cgaaccccgg cttccagacg gaccgccgcc gagcggtcac gcagggaggc
     1621 ctcaatgccc agggggcgaa gaaccgggaa catccgatcc agagccttcg cggggtcctc
     1681 cggattcgcg cgcccggacc gggcccaatt gtccaggagc ccttcggagc cggtgacggc
     1741 caccagcggc atggcctcgc cggtcaactc tttgtgggcc cggcgggagg ccgtctcctg
     1801 ggcccgacgg aaatggctca tcagcggtcc gatttttgcg ggattaaagg tgacccggat
     1861 caatcggggg gcgcccgcgt cgaccccgtc ctttcgttcc agaacctcga catcggcgat
     1921 ggaccgaagc gcccggagcc agagatcggg cccgccctgg gcctgatgcc gaaccaacaa
     1981 ttcggaggcg gccttggtca acaattcggt gagaaaatag ggggattcgg ggagggacgg
     2041 gtcgtttcga aggatccgct cgtccacggt gaccacaaac atcgttggac gaacgccgaa
     2101 aacggggttg gcccggattt cggccggcaa ggaggcctcg aacccgtcga ggtccgcgga
     2161 cgtcaaggtc gccaggcggt cggtgcgggc ggccacgtaa ccgg
//
LOCUS       DAOQLS010000347         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32349_length_2203_cov_3.773277, whole genome shotgun sequence.
ACCESSION   DAOQLS010000347 DAOQLS010000000
VERSION     DAOQLS010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32349_length_2203_cov_3.773277"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PK362_06955"
     CDS             <1..1038
                     /locus_tag="PK362_06955"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNC74604.1"
                     /translation="IHCHNDSDAAVANSLEAVRQGVRLVQGTANGLGERCGNANLISV
                     IPGVMTKLGLPCVSKDQLRSLTEVSRYVDELANQVPNDHQPYVGNSAFAHKGGVHVSA
                     MARHASTYEHMDPGAVGNQRRVLISELAGRSNMILKAREFNLDLEKHPGAIDKIIQQV
                     KAREFNLDLEKHPGAIDKIIQQVKALEKEGYQFEGAEASFYLLVTRLVRPFKPFFDQK
                     GFRVIVEEDKDTGGLVAEASLKIAVDDERVHTVAEGHGPIDALDRALRRALEKFYPNL
                     RSMSLVDFKVRVIDGAVGTASKVRVFVNSRDHAEEWGTVGVSDNIIEASWQALRDAVE
                     YKLLRDGKRPR"
     gene            1181..1969
                     /locus_tag="PK362_06960"
     CDS             1181..1969
                     /locus_tag="PK362_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNC74605.1"
                     /translation="MRILFVGDIFGRPGRDAVRGWVPSLRRERGIDFAIGNAENAAGG
                     RGLTASTAKELFDAGLDVLTLGNHTWDRSETEPLLADPRVLRPANYPAPLPGKGLGVY
                     SVGGRSLAVLQLMGRHNMASIDCPFQAADRLTKDLPTRLVVVDMHAEATSEKQAMGWY
                     LDGRASAVLGTHTHVQTADERVLPGGTAYIGDVGMAGPRDGIIGGDRAGAIKRFLTGV
                     RVRLEVAEGDALFCAVVVDLDDASGRARSIERISKAWERKGGTA"
     gene            1966..>2203
                     /locus_tag="PK362_06965"
     CDS             1966..>2203
                     /locus_tag="PK362_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC74606.1"
                     /translation="MTPQELKKALAAKAAMVDKALDRFMTKEEALPPVVHKAMRYSVF
                     AGGKRLRPVLCLAAAEITGGKPARVLPTACALEVI"
BASE COUNT          391 a          711 c          726 g          375 t
ORIGIN      
        1 atccattgcc acaacgattc cgacgccgcc gtggccaact ccctggaagc cgtgcggcag
       61 ggcgttcgcc tcgtccaggg gaccgccaac ggcctgggcg aacgctgcgg caacgcgaat
      121 ttgatctccg tcattccggg cgtcatgacc aagctgggcc ttccctgcgt gtccaaggac
      181 caactgcggt ccctgaccga ggtgtcccgc tacgtggacg agctggccaa ccaggtgccc
      241 aacgaccacc agccctacgt cggaaattcg gccttcgccc acaagggcgg ggtgcacgtg
      301 tccgccatgg cgcgccacgc ctccacctac gagcacatgg accccggggc cgtgggcaac
      361 caacggcggg tgttgatctc ggaactggcc ggccggtcga acatgatttt gaaggcccgg
      421 gaattcaatt tggatttgga aaagcacccc ggggccatcg acaagatcat ccaacaggtt
      481 aaggcccggg aattcaattt ggatttggaa aagcaccccg gggccatcga caagatcatc
      541 caacaggtta aggccctgga aaaagagggt tatcaattcg aaggggccga ggcgtccttc
      601 tatctgttgg tgacgcggct cgtccgtcct ttcaagccct tctttgacca gaaagggttc
      661 cgcgtcatcg tggaggaaga caaggacacc ggcgggttgg tggccgaagc ctccctgaaa
      721 atcgccgtcg acgacgagcg ggtccacacc gtggcggagg gccacggccc catcgacgcc
      781 ctggaccggg cgctccgccg cgcgctggaa aaattttacc ccaacctccg ctccatgagc
      841 ctcgtggact tcaaagtccg ggttatcgac ggcgcggtgg gtaccgcctc caaagttcgc
      901 gtgttcgtga acagccgcga ccacgccgaa gaatggggca cggtgggggt ctccgacaac
      961 atcatcgaag cttcctggca ggccctgcgc gacgcggtgg aatacaaact cctccgcgac
     1021 ggcaagcggc cccgctgacc ccgattcatc gccgggcggc ccccgcccgg gcccgcccct
     1081 ttttcccgcc gaccgacctc cggacccccc ttttttgcgg tcccggactt ccctccaatt
     1141 tggtatattt tgcctttgat ttatcgtcgg ggagacgccc gtgaggattc tttttgtggg
     1201 ggacattttc ggccgcccgg gccgggacgc ggtgcgggga tgggtaccct cccttcggcg
     1261 cgagcgcggg attgatttcg ccatcggcaa cgcggaaaac gcggccggcg ggcggggcct
     1321 gacggcctcc accgccaagg aactgtttga cgccggactg gacgtgttga ccctgggcaa
     1381 ccacacctgg gaccggtccg agaccgaacc gcttctggcc gacccccggg tgttgcgacc
     1441 ggccaattat ccggcaccgt tgccgggaaa gggcctcggg gtctactccg tgggcgggcg
     1501 gtccctggcg gtccttcaac tcatgggccg gcacaacatg gcgtccatcg actgcccctt
     1561 tcaggcggcc gaccgtttga cgaaagatct gccgacccgg ctggtggtcg ttgacatgca
     1621 cgccgaggcc accagcgaaa agcaggccat gggctggtat ttggacgggc gcgcctccgc
     1681 ggttctgggc acccacaccc acgtgcagac cgccgacgag cgggtgctcc ccggcgggac
     1741 ggcgtacatc ggcgacgtcg gcatggccgg tccccgggac gggatcatcg gcggcgaccg
     1801 ggcgggggcg ataaagcgat ttttgaccgg cgtgcgggtt cgactggaag tggccgaggg
     1861 cgacgcgctc ttttgcgcgg tcgtcgtcga cttggacgac gcctcgggcc gggcccggtc
     1921 gattgaacga atttcaaagg cgtgggaacg gaaaggcgga acggcgtgac tccccaggaa
     1981 ttgaaaaaag cgttggcggc gaaggccgcg atggtggaca aggccctgga ccgcttcatg
     2041 accaaggaag aggccctgcc gcccgtggtc cacaaggcca tgcggtattc cgtgttcgcc
     2101 ggggggaagc gcctgcgacc ggtcttgtgc ctggccgcgg ccgaaatcac gggggggaaa
     2161 ccggcccggg tcctgccgac ggcttgcgcg ctggaggtca tcc
//
LOCUS       DAOQLS010000348         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32452_length_2199_cov_3.431437, whole genome shotgun sequence.
ACCESSION   DAOQLS010000348 DAOQLS010000000
VERSION     DAOQLS010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32452_length_2199_cov_3.431437"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="PK362_06970"
     CDS             complement(<1..2004)
                     /locus_tag="PK362_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNC74607.1"
                     /translation="MTENTPTLPGGSEAWWLLTRAADLGRTLVVCATDEAAQNLFDDH
                     TALARWKSPESPPTARFFSEEEEERLAALSAWSGPDASANFLIASLDSLTVPLPAPGT
                     FFGDHQKVSLGDRTNRDEFFGKLMNQGYERVDTVEKIGEVAVRGEVMDLWSPGWEGPL
                     RLLWPFDQIESLRKIDLNTQRSTDEILEEWVRPARLKDEGHTLLDFLKDGTLFESRLP
                     PGAAPARDGRRVVHAPLEPTAAAPGYEPVPPFAGRADLLARTVAEWLATGWRVLIFCH
                     NAGEQERVEELLLEQDRGLAEAFETRRIDLPLGHLVHGFLDPGEKRAVLSNGEIFGRI
                     RRRLRLPKFVGGASLGQIAELKKDDYVVHGEFGIGRYVGLERKNAGGVEMDYLRLEYR
                     GGDRVFVPLFEFRQVKKYIGVEGKRPKLSSLDTATWDRTKAEVEEAVAQLAKDLLARA
                     AARTSRPGYAFPPDSHMEKEFAASFVYDLTPDQARAIEETKRDMMRPMPMDRLVCGDV
                     GYGKTEVAMRAALKAVSAGKQVALLVPTTILAEQHGRNFRDRFADYPVSVAVLSRFSA
                     PAERKRVIQDVRRGVIDIIVGTHALLSPEVVFKDLGLLIIDEEHRFGVKQKERFLAFR
                     DVVDVLALSATPIPRTMGSALGGLKALSVIETPPEGRLPIQTHL"
BASE COUNT          368 a          676 c          734 g          421 t
ORIGIN      
        1 caggtgggtt tgaatgggca ggcgtccctc gggcggcgtt tcgatgacgg agagggcctt
       61 gaggcccccc agggccgagc ccatggtgcg gggaatgggc gtggccgata gggccaggac
      121 gtcgacgacg tcccgaaagg ccaggaaacg ctctttttgt ttgacgccga accggtgctc
      181 ctcgtcgatg atcaggagcc ccaagtcctt aaagacgacc tcgggggaaa gaagcgcgtg
      241 ggtgcccacg atgatgtcga tgacgccccg ccggacgtcc tgaatgacgc gcttccgttc
      301 cgccggggcc gaaaagcggg aaagcaccgc cacgctgacg gggtagtcgg cgaagcgatc
      361 gcggaagttt cgcccgtgct gttccgccaa aatcgtcgtg ggcaccagaa gcgccacttg
      421 tttcccggcg gagacggcct tcagggccgc gcgcatggcc acttcggttt ttccgtagcc
      481 cacgtcgccg cagaccaatc ggtccatggg catcggacgc atcatgtccc gtttggtttc
      541 ctcgatggcc cgggcctgat ccggcgtcag gtcgtagacg aaggaggccg cgaattcctt
      601 ttccatgtgg ctgtcgggag ggaaagcgta accgggccgg gaggtccggg ccgccgcccg
      661 ggccaacagg tccttggcca gctgggccac ggcctcttcc acctcggcct tggtgcggtc
      721 ccaggtggcg gtgtccagag aggagagttt gggccgtttt ccctccacgc cgatgtactt
      781 cttgacctgg cggaattcaa agaggggcac aaaaacccgg tcgccgcccc ggtactcgag
      841 ccgcaggtaa tccatctcga ccccgccggc gttttttcgc tccaacccca cgtagcgtcc
      901 gatgccgaat tccccgtgga ccacgtaatc gtcttttttt agctcggcga tttggcccag
      961 ggaggccccg cccacgaatt tgggcaggcg caggcgccgt cgaatccggc cgaatatctc
     1021 cccgttcgac aggaccgccc gtttttcccc gggatccaaa aacccgtgca ccaggtggcc
     1081 caggggcagg tcgatgcggc gggtttcaaa ggcctcggcc aggccgcggt cttgttccaa
     1141 caaaagttct tccacccgtt cctgttcgcc ggcgttgtgg caaaagatca agacccgcca
     1201 acccgtggcc agccattccg ccacggtccg ggccaacagg tcggcccggc cggcgaaggg
     1261 ggggacgggt tcgtaaccgg gggccgcggc cgtgggttcc agcggcgcgt ggacgacgcg
     1321 ccgtccgtcg cgggccgggg cggctcccgg cggcaggcgg gattcgaaga gggttccgtc
     1381 tttcaggaaa tcgagcaggg tgtgcccctc gtctttcaag cgcgcgggtc ggacccattc
     1441 ctcgaggatt tcgtcggtgg accgttgggt gttgaggtcg attttccgca gggattcgat
     1501 ctggtcgaag ggccagagaa gccgcagggg gccctcccac ccgggggacc agaggtccat
     1561 cacttcgccg cgcaccgcca cctcgccgat tttttcgacg gtgtccacgc gttcgtaccc
     1621 ctggttcatg agctttccga aaaattcgtc ccggttggtc cggtccccga gggacacttt
     1681 ttgatggtcg ccgaaaaagg tcccgggcgc gggcaggggg acggtcaggc tgtccaggga
     1741 agcgatcaga aagttcgcgg aggcgtcggg accgctccag gcggacaggg cggcgaggcg
     1801 ttcctcttcc tcctcggaaa agaaccgggc cgtcgggggg gattccgggg atttccatcg
     1861 ggccagggcc gtgtggtcgt cgaagaggtt ctgcgcggcc tcgtccgtgg cgcagaccac
     1921 gagggtccgt ccgaggtcgg cggcgcgggt cagcagccac caggcttcgg atcctcccgg
     1981 gagcgtcgga gtgttttcgg tcaatgtatt ttccatgatc ggcttaaatt tcttctcgtt
     2041 ttaccttctt cccttgaagg ggaaggtcag gatgaggggt gattttgaat ttctcataaa
     2101 ttcaaagtcg tgggccttcc aggcccacac ccggaaaatc caagtcgccg ggggttgccc
     2161 ggcggcaacc tacttttctt tggccgccca aagaaaagt
//
LOCUS       DAOQLS010000349         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32569_length_2194_cov_3.564282, whole genome shotgun sequence.
ACCESSION   DAOQLS010000349 DAOQLS010000000
VERSION     DAOQLS010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32569_length_2194_cov_3.564282"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK362_06975"
     CDS             complement(<1..258)
                     /locus_tag="PK362_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNC74608.1"
                     /translation="MPTLRLALAQVNSTVGALGANADHLLFRARAARDRGADLVLFPE
                     MALTGYPPEDLLLEPLFLKSTADALKALARALPRDLAVVLGA"
     gene            complement(276..1514)
                     /locus_tag="PK362_06980"
     CDS             complement(276..1514)
                     /locus_tag="PK362_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC74609.1"
                     /translation="MPRAKTTAGPLDPAEELELLHKISHLIGSTLELKTILQEIVGLV
                     SGLTKADATFLYLHEPAQGQLVLSAAKPPHPSEVGQLRLKMGEGLTGWVAEHKKPLVL
                     PQKAHDDHRFKYFQALPEDRFEAFLSVPILVREDVVGVINVQHRKPHPYSEHTLKILA
                     TIGRQVGSAIENGRLFEETKRRANAIQTLSAVSHTVASDRFHEEILQLIVAMTASLMG
                     SKICSVMLLNEEGTELRITATQSLSPAYRNKPPIKVDQSLSGQAVLQKKPVAVMDVRK
                     DKRFSFPDVAASEGLVSLLSVPMLYKDKALGVINAYTAEEYAFTKEDISVLQSVANQC
                     ASAIMQTRLLQEKLAAQDALESRKTIERAKGILMKKRGMTEPEAFREIQKQSMDRRKT
                     MKEIAEAVLLAEELGGAGKA"
     gene            1638..>2194
                     /locus_tag="PK362_06985"
     CDS             1638..>2194
                     /locus_tag="PK362_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74610.1"
                     /translation="MDRFEKSGRRCARFFLRRGAALALVGLGLWPLWAGNEPRLRKAD
                     PFLLYWAQFRRNVMEEDWEKLSFLVQFPLATERGPRPKDQFAGLIPRILDAQIPGGDY
                     PTTTRALVTEKSQLSDEDWETIHGDSYEIGKLHFAKVNGRWALTRVGVAPAPKTPPPV
                     EEPPTGTPSAPPVVEPTPEPPAAAPV"
BASE COUNT          384 a          623 c          748 g          439 t
ORIGIN      
        1 ggcgcccagg accacggcca agtcccgggg cagggcgcgg gcgagggctt tcaaggcgtc
       61 ggccgtactt ttaaggaaga ggggttccag caacaggtct tcgggggggt aaccggtgag
      121 ggccatttcg gggaacagga cgaggtccgc gccccgatcg cgggccgccc gggcccggaa
      181 cagcagatgg tcggcgttgg cgcccagggc gcccacggtg gaattgacct gcgccagggc
      241 gagacgaagc gtgggcatgc gtcccgcgcg gggaatcagg ctttcccggc gccgcccagc
      301 tcttcggcca ggaggacggc ttcggcgatt tccttcatgg ttttccggcg gtccatgctt
      361 tgtttttgga tttcccggaa ggcttcgggt tcggtcatgc cgcgtttttt catcaggatg
      421 cccttggccc gttcgatggt ttttcgggat tccagagcgt cctgggccgc gagtttctcc
      481 tgaaggaggc gcgtttgcat gatggccgag gcgcattggt tggccaccga ctgaaggacg
      541 gagatgtcct ccttggtgaa ggcgtattcc tcggcggtat aggcgttgat cacgcccagg
      601 gctttgtctt tgtagagcat ggggaccgac agaagcgaga cgagcccctc ggacgcggcg
      661 acgtcgggga aggaaaaacg tttgtctttt cggacgtcca tgacggccac gggtttcttc
      721 tgcaggacgg cctggcccga aaggctttgg tccaccttga tcggaggttt gttccggtag
      781 gcgggggaga gggactgggt ggccgtaatc cggagctccg ttccctcctc gttcaacagc
      841 atgaccgagc agattttgga gcccatcaag gacgccgtca tggccacaat caactgaagg
      901 atttcctcgt ggaagcggtc cgaagccacg gtgtgggaaa ccgccgaaag ggtctgaatg
      961 gcgttggccc gccgcttcgt ttcctcgaac agccggccgt tctcgatggc gctgccgacc
     1021 tggcgtccga tggtggccag tattttgagc gtgtgctcgg agtagggatg gggtttgcgg
     1081 tgctggacgt tgatgacgcc cacgacgtcc tcccggacga ggatgggcac cgagaggaac
     1141 gcttcaaaac ggtcctcggg cagggcctgg aaatatttaa aacggtgatc gtcgtgggcc
     1201 ttttggggga ggaccagggg ttttttgtgc tccgccaccc agccggtcaa tccttcgccc
     1261 attttaaggc gcagttggcc cacttcgctc gggtgcgggg gtttggcggc ggagaggacc
     1321 agttggccct gggcgggttc gtgaaggtaa aggaaggtgg cgtcggcttt ggtcaggccg
     1381 ctcaccagcc ccacgatttc ctggaggatg gtcttcaatt ccaacgtgga gccgatgaga
     1441 tgggaaattt tgtggaggag ctccagctcc tcggccgggt ccaaagggcc ggccgtcgtt
     1501 ttcgcgcgtg gcatgggttc tcctggtggg ggaaaggttg aaaaaaggaa aataccatct
     1561 ttccgacggt ccgtcaaagg ggtgaggagg ctccggggcg cgttcccaag gcgggccggg
     1621 gatggtaaaa taaccccatg gaccgtttcg aaaaatccgg tcgtcgctgt gcccgatttt
     1681 tcctccgacg cggggccgct ctggctttgg tgggcctggg cttgtggccc ctgtgggccg
     1741 gcaacgagcc ccgccttcga aaggccgatc cctttttgct ttattgggcc caatttcgac
     1801 gaaacgtgat ggaggaggat tgggaaaaac tgtcctttct cgttcaattt cccctggcga
     1861 ccgagcgggg gccccggccc aaagaccagt tcgccggatt gattccccgg atccttgacg
     1921 cccaaatccc cggcggggat tacccgacga ccacccgggc gctcgtcacg gaaaaaagcc
     1981 aattgagcga cgaggactgg gaaacgattc acggggacag ctacgaaatc ggaaaacttc
     2041 attttgcgaa ggtgaacggg cgttgggcct tgacccgcgt gggcgtagcg cccgcgccga
     2101 aaacgccccc gcccgtggaa gaacccccga ccgggacgcc ctccgccccc ccggtcgttg
     2161 aaccaacgcc cgaacccccg gcggccgccc ccgt
//
LOCUS       DAOQLS010000350         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32611_length_2192_cov_5.530182, whole genome shotgun sequence.
ACCESSION   DAOQLS010000350 DAOQLS010000000
VERSION     DAOQLS010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32611_length_2192_cov_5.530182"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PK362_06990"
     CDS             <1..306
                     /locus_tag="PK362_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74611.1"
                     /translation="ENTPRPIQLLLTDLIMPRMRGSQLAERLAKIRPDLKILFMSAHR
                     DHEPPRKDMPALNHPLLLKPFRPAELAQRVRELLDGSAPGGPSHSADAGPTAVQPAA"
     gene            complement(370..444)
                     /locus_tag="PK362_06995"
     tRNA            complement(370..444)
                     /locus_tag="PK362_06995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Glu,seq:ttc)
     gene            complement(495..1202)
                     /gene="nfi"
                     /locus_tag="PK362_07000"
     CDS             complement(495..1202)
                     /gene="nfi"
                     /locus_tag="PK362_07000"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303249.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HNC74612.1"
                     /translation="MKPHNLHPWNVPIDQARGIQEELAQRVDLENTVDDVRTVAAVDI
                     ALDGKTRGVAAAVLMAFPGFEVLETSVVRGDLTFPYVPGLLSFREGPLLIDALLRLRR
                     EPDVIFFDGQGVAHPRRLGVASHLGLVLDKPTVGLAKSRLVGVFKEPGPEEGAASDLV
                     FQGRLVGYAVRSRANAKPIFVSPGHRIDYQTALRLATASFDGQRVPRPARWADGLSKA
                     AKRGEAKKFIAALKKKN"
     gene            complement(1271..1345)
                     /locus_tag="PK362_07005"
     tRNA            complement(1271..1345)
                     /locus_tag="PK362_07005"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1311..1313),aa:Lys,seq:ttt)
     gene            1450..1776
                     /locus_tag="PK362_07010"
     CDS             1450..1776
                     /locus_tag="PK362_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74613.1"
                     /translation="MTADRFLYNTALHPKGPQALPTFAIRDGRVFTTVHHSDGADPHP
                     WYEVREDRLYPTDHHPQRAPKHRPWYSIRGNNVFADEGHPHGAQADAWFELRDQPGSR
                     GGIAHG"
     gene            1769..2164
                     /locus_tag="PK362_07015"
     CDS             1769..2164
                     /locus_tag="PK362_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74614.1"
                     /translation="MDKKEPNDGKDPKRKHLPKLTKKKLDHFLASICEAAGMTGKTPK
                     KPVSKGGLTCQGPCEKSHWRAHLDIPSGMLCVSADWSKNRGAEKHRDVTIAITYLDER
                     GKKQYSEMIFCDSEVRWLMETLWHAPIWE"
BASE COUNT          433 a          715 c          667 g          377 t
ORIGIN      
        1 gaaaacaccc cccggcccat tcagttgttg ttgaccgact tgatcatgcc gcgcatgcgg
       61 gggtcccaat tggcggagcg attggccaaa atccgcccgg atcttaaaat tttgtttatg
      121 tccgcccacc gggaccacga gcccccgcgg aaggacatgc cggccctgaa ccacccgttg
      181 ttgctgaaac cctttcgtcc cgccgagctg gcccagcgcg tccgggaatt gctggatgga
      241 agcgcgcccg gcggcccttc ccactcggcg gacgcgggcc ccaccgccgt tcaaccggcg
      301 gcctagtcga cgggggtgga aaaaaaagac ccccctccgt tggaaggggg gtctttcggg
      361 aaatgccatt ggtgtcccca aggggatttg aacccctgct acggccttga aagggccgtg
      421 acctaaccgc tagtcgatgg ggacacccat ggcacttgcg cgtcaaaaag agcgttcggg
      481 cggccggccc ggacttagtt cttttttttc aaggccgcga tgaatttttt cgcttcgccg
      541 cgtttggccg ccttggaaag tccgtccgcc caccgggcgg gacgcggcac cctctggccg
      601 tcgaagctgg cggtggccaa ccgcagggcc gtttggtaat cgatccgatg gccgggcgag
      661 acaaaaatcg gtttggcatt ggcccgggac cgcaccgcgt aacccaccag gcggccttgg
      721 aatacgaggt ccgacgccgc gccctcctcg gggccgggtt ctttgaagac ccccaccaac
      781 cgggatttgg ccaggcccac ggtcggcttg tccaacacca ggcccaggtg ggaggccacc
      841 cccaggcgcc gcggatgggc cacgccctgg ccgtcaaaaa aaatcacgtc gggttcccgg
      901 cgaaggcgaa gaagggcgtc gatcaacagc ggcccctcgc gaaaactcaa aaggcccggc
      961 acgtagggaa acgtcagatc gccgcgaacc acggaggttt ccagcacctc gaacccggga
     1021 aaagccatca gcacggcggc cgcgactccg cgggttttcc cgtccaaggc gatgtccacc
     1081 gcggcgacgg tgcgaacgtc gtcgacggtg ttttccagat cgacccgctg ggccagctct
     1141 tcctgaatcc ctcgagcttg gtcgatcggt acgttccacg ggtgaaggtt gtgcggtttc
     1201 aaatccggtt cggtcccgcg gcggtcgggc cggtttcgtc gttcccgggc cgggcgccgc
     1261 gcgtttcaaa tggtgggtcc ggagggattt gaaccctcgg cctctcgctt aaaaggcgaa
     1321 tgctctaccc ctgagctacg gaccccgtcg ggttcactcc ccgaggggcg gattgtagca
     1381 gttttgcgtg cggagacaaa tccaccgacg gctggagaaa ccccgcgatt catgataaaa
     1441 tgagtcaaga tgaccgccga ccggttcctg tataacacgg ccctgcaccc caaagggccc
     1501 caggcccttc ccaccttcgc gatccgcgac gggcgggtct tcacgacggt gcatcattcc
     1561 gacggggccg atccccaccc ctggtacgag gtgcgggagg accggctgta tcccacggac
     1621 caccaccccc agcgcgcgcc gaaacaccgg ccgtggtatt ccatccgggg gaacaacgtt
     1681 tttgccgatg aggggcaccc tcacggcgcc caggccgacg cttggttcga gctccgggat
     1741 caaccggggt ctcgcggagg aatcgcccat ggataaaaaa gagcccaacg acggcaaaga
     1801 ccccaaacgg aaacaccttc ccaaactgac caagaagaaa cttgatcatt ttttggcgtc
     1861 gatctgcgag gcggcgggca tgacgggcaa aacgcccaag aaacccgtgt ccaagggggg
     1921 cttgacctgc cagggcccct gcgaaaaatc ccattggcgc gcccacctcg acatcccctc
     1981 gggcatgttg tgcgtgtccg ccgattggtc gaagaaccgg ggcgccgaaa agcaccggga
     2041 cgtcaccatc gccatcacct atttggacga gcgcggaaag aaacagtatt ccgaaatgat
     2101 tttctgcgat tccgaagttc gctggctgat ggaaacgctc tggcacgccc ccatttggga
     2161 ataagcgcgc cggttcgatg acccacgccg cc
//
LOCUS       DAOQLS010000351         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32623_length_2192_cov_3.946654, whole genome shotgun sequence.
ACCESSION   DAOQLS010000351 DAOQLS010000000
VERSION     DAOQLS010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32623_length_2192_cov_3.946654"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PK362_07020"
     CDS             <1..1253
                     /locus_tag="PK362_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74615.1"
                     /translation="TERLSALRALNLDRAELLLSADDLKAKAAKLKAALNQAAAERML
                     ARLIDEANGRAVDLSRLGRDAEFTQATLTDLGNKVFQPLFARTLGLSASTNLPEMKVN
                     WGPTPSKANAARAWMVSRWATNGVRNEVDVRGTDPLAITLALLHESAHAVLMAAWGRP
                     MSQERLDMEALAGALTQMALGTRADLQPLAQFEQSESRFFAGQNRPELWRAREIAHGL
                     LAKALTLGAAKGEALRSILASAQEEGGLLAMALGAGLGKSSTQSTEPLLFMVSASDLI
                     ARGKLQEEAIRNLESLAAKLSATSDTKEYRAVVVNNLGQRMGDWKKITSRLKGKGVLT
                     IDKAPAGAVQDRTNKVVLARLMPALPGLLGLARSAKLRIAILTGAQDTWIADKDQVVF
                     FMRLTISEFVDKAFEAMKVAGRSA"
     gene            complement(1748..>2192)
                     /locus_tag="PK362_07025"
     CDS             complement(1748..>2192)
                     /locus_tag="PK362_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74616.1"
                     /translation="DRWGLSAQRILTLGFDGLSKANAIEFAPGGRRVKMEALKSEMAR
                     QTGGGNGQAFRLTLWAASGRAEIFDGDLVRLIAVEQDLLLEGAAATAFVERLRQLFGS
                     DEAAQMIRDLSPDGTGRVIRLPGRSPLLQETINAIQTDLLIQTNA"
BASE COUNT          426 a          692 c          687 g          387 t
ORIGIN      
        1 acacggaacg tctctccgcc cttcgggccc tgaacttgga ccgcgcggaa ttgctgttga
       61 gcgccgacga cctgaaagcc aaggccgcca aattgaaagc ggccctgaac caggcggccg
      121 ccgaacggat gctggcccgt ctgatcgatg aggccaacgg ccgcgccgtg gacctgagcc
      181 ggttgggccg ggacgccgaa ttcacccagg cgacattgac cgacctgggc aacaaggtgt
      241 tccaaccgct ctttgcgcgc acgctgggcc tttcggcgtc gacgaacctg cccgagatga
      301 aagtgaactg gggtcccacc ccgtccaagg ccaacgcggc gcgggcgtgg atggtcagcc
      361 gctgggcgac caacggcgtc cgcaacgaag tggacgtccg cggaacggac ccgctcgcca
      421 tcaccttggc cctcctgcac gaatcggccc acgccgtcct catggcggcc tggggacgtc
      481 ccatgagcca ggaacgcctg gacatggagg ctctggccgg cgccttgacg caaatggcct
      541 tgggaacgcg ggcggatctc caaccgttgg cccaattcga gcagtccgag tcccggttct
      601 tcgcgggcca gaaccggccc gaactgtggc gggcccggga aatcgcccac ggcctcctgg
      661 ccaaagccct gactttgggc gccgccaagg gcgaggccct tcggtccatc ctggcttcgg
      721 cccaggaaga gggcggcctc cttgccatgg ccctgggcgc gggactgggc aaatcgtcca
      781 cccaatccac cgaacccttg ctgttcatgg tttcggcctc ggacttgatc gcccgcggca
      841 aactgcagga agaagccatt cgaaacctgg aatccttggc cgccaaactg agcgcgacgt
      901 cggacaccaa ggaataccgg gccgtggtgg tgaacaacct tggccaacga atgggcgatt
      961 ggaagaaaat cacttcccgg cttaaaggca aaggcgtgtt gaccatcgac aaagcgccgg
     1021 ccggcgccgt gcaggaccgg accaacaagg tggtcctggc gcggttgatg ccggccttgc
     1081 ccggtcttct gggcctggcg cgcagcgcca aactgcgcat cgccatcttg accggcgccc
     1141 aggacacctg gatcgcggac aaagaccaag tggtcttctt catgaggctc acgatcagcg
     1201 agttcgtgga caaggctttc gaggcgatga aggtcgccgg tcgcagcgcc taacgagaag
     1261 cgcctaacac cttcttcaac cggaaacccc ggttcgccgt aaaggcgggc cggggtttct
     1321 tcttttcttc gcctgcccag ggggaccatc tccccgccgg ttccatccga gaacgtccgc
     1381 ctcgatgagg tcccttcggt tcaacggtta ggggcgcatt gggggtaggt tccaggtcga
     1441 caatcttcag gaagtggcga tccattaacg gagaacgccg catggatccg gagggggagc
     1501 ggtccgttgc tcgatgttca attcctggaa agggagggcc cttccgtttc gaaaagcggc
     1561 tttgtaaaac tcttgcgggc aacgaaggtt gtcttcgacc cgggacatag cggtcctcgg
     1621 ctgacccagc ctcaaggaaa atacgactcg agtcggcgcc acgggtggcc ctttacctga
     1681 gcgatgctcg ggaaccgccg acggcggcca agcttcgagg tcgaccggga accggtcgag
     1741 gcaatttcta ggcgttggtt tgaatcagta ggtcggtttg aattgcgttg atggtttctt
     1801 gaagaagggg ggaacgcccg ggcaagcgga tcacccgacc cgtgccgtcg ggggataaat
     1861 cgcgaatcat ttgggccgct tcgtcgctgc caaacaattg gcggagacgt tcaacaaagg
     1921 ctgtggccgc cgccccttcc aatagcagat cctgttccac ggcaatgagg cgtaccaaat
     1981 cgccgtcaaa gatctccgct cggccggaag cggcccaaag cgtcaaccgg aaggcctggc
     2041 cgttcccgcc ccccgtttgc cgcgccattt cggatttgag ggcctccatt tttacccttc
     2101 ggccgcccgg agcaaattca atggcgttcg cttttgaaag gccatcgaat cccagggtca
     2161 gaatccgttg ggccgacagg ccccagcgat cg
//
LOCUS       DAOQLS010000352         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32769_length_2187_cov_2.889306, whole genome shotgun sequence.
ACCESSION   DAOQLS010000352 DAOQLS010000000
VERSION     DAOQLS010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32769_length_2187_cov_2.889306"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..796
                     /locus_tag="PK362_07030"
     CDS             341..796
                     /locus_tag="PK362_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74617.1"
                     /translation="MATTTPLDTEFARLAGDMAEEFNFNRSLGQIYGLLYMQDAPLAL
                     EDIGRRLSMSKGNVSINIRQLEAWGAVRPLSVVGSRKDYYEANRDIKAVALRRLNEGL
                     TKRLDHAERRMAALLEAPVDPAHRKRIQELETLLSRGRRALKFAAQWFG"
     gene            784..1830
                     /locus_tag="PK362_07035"
     CDS             784..1830
                     /locus_tag="PK362_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNC74618.1"
                     /translation="MVRLTGPCAHGILPPFLLERMAESPDPATRKWALKSLAASAAAR
                     AYRRALATQPAMAAIPSPEGKKHRLVYDAKNKETLPGKLIRAEGQKGSKDPAVNEAYD
                     FSGATYDFYDKLFSRNSLDDRGMSLISSVHVGRSYANAFWNGEQMAYGDGDGRVFTRF
                     TKALDVVGHELTHGVVTHTANLDYEDEPGALNEHFADVFGLLVKQWRRKETAARADWL
                     IGGDLLVKKPTRRALRSFAAPGTAYKNDPDLGTDPQPAHMKNKYTGEDDNGGVHINSG
                     IPNHAFYLAARKIGGRAWEKTGKIWYGALRALSPKSGFREAAMATTEKAAALYGRAEA
                     AAVKAAWTAVGISR"
     gene            1827..>2187
                     /locus_tag="PK362_07040"
     CDS             1827..>2187
                     /locus_tag="PK362_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74619.1"
                     /translation="MRTFVPRDFVPSPLFHFAQGGRRRWPLAFLCAFLPACATLNPEA
                     FKNFQSAVAAEQKGLETEMARDVDWTREADVQDLANRKDAPLSAYMVKEAKAYEWTMD
                     ALPPHWDARRTLRTLEEF"
BASE COUNT          431 a          744 c          656 g          356 t
ORIGIN      
        1 ccgtcctcta ttatacggga cggcctggtc tattccttcg tccttaaagg aaaccgacgc
       61 gtgacctttc aggaaaccgc cctgcccccg tcccttcgga cgttggtggc ccgcgtcact
      121 cgccgtcggc cggaccggga aggagattcc gcctgggcgt gaagcgcgtc tgtcccgatc
      181 gacttggcca ctaaaaaata aacgagtcca atcggaatcg cccgcttatg gcgggggtgg
      241 cccgggggtt ggcggggcgg tccgccaccg acgagcgggg gccttgacaa cgggtcgttc
      301 tcattgtatg tttagaacgg attaaacgtt gaaaacatac atggcgacca ccacccccct
      361 cgatacggaa ttcgcccgcc tggccggcga catggcggag gagttcaatt tcaaccgctc
      421 cctgggccag atttacggtc tcctctacat gcaggacgcg cccctggccc tggaagacat
      481 cggccggcgg ctgtccatga gcaagggcaa cgtcagcatc aacatccgtc aattggaagc
      541 ttggggggcc gtccggcctc tttccgtcgt gggaagccgg aaggattatt acgaagccaa
      601 ccgggacatt aaagccgtgg cccttcggcg gttgaacgag gggctcacca agcgcctgga
      661 ccacgcggag cggcggatgg cggccctgct ggaagccccc gtcgatcccg cccatcgtaa
      721 gcgcatccag gaactggaaa ctctcctctc ccggggacgc cgggccctca agttcgccgc
      781 ccaatggttc ggttaaccgg tccctgcgcc cacggcattt tgccgccgtt ccttttggag
      841 cggatggcgg aatccccgga tcccgcaaca cgaaagtggg cgctcaaaag cctggcggcc
      901 tcggccgcgg cccgggccta tcggcgggcg ctcgcgaccc agcccgccat ggcggccatc
      961 ccctcccccg aaggaaaaaa gcaccgcctg gtttatgacg ccaaaaacaa agaaacgttg
     1021 cccgggaaac tgatccgcgc cgaagggcaa aaggggtcga aggaccccgc ggtgaacgaa
     1081 gcctacgact tttccggcgc gacctacgac ttttacgaca aactgttttc ccggaactcc
     1141 ctggacgacc ggggcatgtc cctgatctcc agcgtccacg tggggcgaag ctacgccaac
     1201 gccttttgga acggcgagca gatggcctac ggcgacggcg acggccgcgt cttcacgcgg
     1261 tttacgaaag cgctggacgt cgtcgggcac gagttgaccc acggggtcgt gacccacacg
     1321 gccaacttgg attacgagga cgagcccggc gccctgaacg agcatttcgc cgacgtcttc
     1381 ggcctgttgg tcaagcagtg gcggcggaag gaaaccgccg cccgggccga ttggctcatc
     1441 ggcggcgacc tgctggtgaa aaagcccacc cgacgggccc ttcgcagttt cgccgccccc
     1501 ggcaccgcct acaaaaacga tcccgatctc ggcaccgacc cccagccggc ccatatgaaa
     1561 aacaaataca ccggcgagga cgacaacggc ggcgtgcaca tcaactccgg gattcccaac
     1621 cacgcctttt acctggccgc ccggaaaatc ggcggccggg cctgggagaa aacgggaaaa
     1681 atttggtacg gggcccttcg ggccctctct cccaaaagcg gtttccgcga ggcggccatg
     1741 gccacgacgg aaaaagccgc ggccctttac ggccgcgccg aagccgccgc cgtcaaagcc
     1801 gcttggaccg ccgtgggaat ctcccgatga ggaccttcgt cccccgggac ttcgtcccgt
     1861 ccccgctttt ccacttcgcc caaggcggtc gtcggagatg gcccctggcg tttctttgcg
     1921 cctttcttcc cgcctgcgcc acgctcaacc ccgaggcctt taaaaatttc caatccgccg
     1981 tcgcggccga acaaaagggc ctcgaaaccg aaatggcccg ggacgtggac tggacccggg
     2041 aggccgacgt ccaggacctg gccaaccgga aggacgcccc cttgagcgcc tacatggtga
     2101 aagaggccaa ggcctatgaa tggaccatgg acgccctccc gccccactgg gacgcccggc
     2161 gcacgctccg gaccctggaa gagttca
//
LOCUS       DAOQLS010000353         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_32791_length_2186_cov_3.731112, whole genome shotgun sequence.
ACCESSION   DAOQLS010000353 DAOQLS010000000
VERSION     DAOQLS010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32791_length_2186_cov_3.731112"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PK362_07045"
     CDS             complement(<1..625)
                     /locus_tag="PK362_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNC74620.1"
                     /translation="MTGSRAFPAPAKINLFLEIVRRRPDGYHTLSTLFQEISLADTLR
                     ARPDPSGGPRLFCSFPGLTVGEDNLVLRAARAYATGDPKAVPLRFDLAKKIPLGAGLG
                     GGSSDAAAALRAQWFFSRGGRPFPEKRFRLLARRLGADVPFFLRGGLAEGRGVGEKLE
                     FLSPRREEVRHFVLVYPRVFSSTPEAYRALRFPLTKRRSGLKLKRALI"
     gene            complement(622..>2186)
                     /locus_tag="PK362_07050"
     CDS             complement(622..>2186)
                     /locus_tag="PK362_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74621.1"
                     /translation="GWKRIAERCWLNAPDFTGVAGYEDYVLRVADNPEVNARLGQLYF
                     QAGDMDRARSAFDRAAQSGPRDPVVNFWRALVAQEEERWADAAQFMAQAARVAPQTGV
                     LLRLASYYQRLGRPRDTVKTLARLQKAHPENPDFMQYLGQAQEDNGRPRQAIAWYERA
                     LKIDPKRADVQFRLAMNWDRLHRFDKALPALRNAIAADPRHHLALNYLGYSLAERGEN
                     LPEALDLIQRAVALAPDNAAYRDSLGWAYFRLGRLAEAETALAASVHPADDPAVWLHY
                     GDVLEARGKKEESMRAWQEGLLLDPNNRDLLTRLGDEGRIKNVAPRTAARTLLKRVEG
                     NFRQLRGLSGFVEVDGRWRGRAVAGRGVWHYARPGLFRLEILGPLFAPAAVMVHTSSG
                     TQWYPPELGDPAVDGWLALLNRVLSGDWTAGFDDPAVTVRLEGDEVVYDRPSGTTILR
                     ARTKTLSEARVPSPEGPLRVRFERVVEKEGLRVPTVLRLESEAGSLTLTFTQILVNPP
                     RAGDLFSLPAAP"
BASE COUNT          398 a          723 c          736 g          329 t
ORIGIN      
        1 cgatcaaggc tctttttagt ttaagccccg aacggcgctt tgtcaacgga aatcgaaggg
       61 cccggtaggc ctcgggcgtg gaggaaaaca cccggggata aacgaggacg aaatggcgga
      121 cctcctcgcg ccggggcgac agaaattcca atttttcgcc gaccccccgt ccttcggcca
      181 gccccccccg caggaaaaag ggcacgtcgg ccccgaggcg gcgagccaag aggcgaaagc
      241 gcttttccgg aaagggacgg ccgccccggg aaaaaaacca ttgggcgcgg agagccgccg
      301 ccgcgtcgct ggaaccgccg ccgagccccg ccccgagcgg aattttcttc gccagatcga
      361 accggagcgg aacggctttc gggtccccgg tcgcgtaggc ccgggcggcg cggagcacca
      421 aattgtcttc gccgacggtc aaccccggga aggagcagaa cagtcggggg cccccggacg
      481 ggtccggacg ggcccgcagg gtatcggcca gggaaatttc ctgaaaaaga gtggacaggg
      541 tgtgatagcc gtcgggacgc cggcgaacga tttcgagaaa caggttgatc ttcgccgggg
      601 cggggaaagc gcgggatccg gtcacggcgc ggcgggcaac gaaaacaaat cgcccgcccg
      661 gggcgggttc accaggatct gcgtgaaggt caaggtgagg cttcccgcct cggattccag
      721 gcgaaggacg gtgggcaccc gaagcccctc tttttcaacg acgcgctcga accggacccg
      781 gagcggcccc tccggcgatg ggacccgggc ctccgacagg gtcttggttc gggcccggag
      841 gatcgtggta ccggacggac gatcgtaaac cacctcgtcc ccttccaatc ggacggtcac
      901 ggccgggtcg tcgaacccgg cggtccaatc ccccgacaga acccggttca aaagggccag
      961 ccagccgtcc acggccggat cgccgagttc cggcggatac cattgggtgc cggaggacgt
     1021 gtgaaccatc acggccgccg gcgcgaagag gggccccaga atttccagcc ggaaaaggcc
     1081 gggccgcgcg tagtgccaga ccccccgccc ggccacggcg cgcccgcgcc aacgcccgtc
     1141 cacttcgacg aatccggaaa ggccgcgaag ctgacggaag ttcccctcca cgcgtttcaa
     1201 caacgttcgg gccgccgttc ggggggccac gtttttgatc cggccttcgt cccccaggcg
     1261 cgtcaacaaa tccctgttgt tcgggtccaa cagcaggcct tcctgccagg cccgcatgga
     1321 ttcctccttc ttgccccggg cctccaaaac atcgccgtaa tgcagccaca ccgcgggatc
     1381 gtcggcggga tggacgctgg cggccaaggc ggtttcggcc tcggccaacc ggcccaggcg
     1441 gaaataggcc catcccaggg agtcccggta ggcggcgttg tcgggcgcca gggccacggc
     1501 ccgctgaatc aagtccaggg cttccggcag gttttccccc cgttcggcca ggctgtagcc
     1561 cagataattc agggccaaat ggtggcgggg gtccgcggcg atggcgtttc gaagggcggg
     1621 aagggccttg tcgaagcggt ggagccgatc ccaattcatg gccaagcgaa attgcacgtc
     1681 ggcgcgtttg ggatcgattt tgagggcccg ctcgtaccag gcgatggcct ggcggggccg
     1741 gccgttgtct tcctgggcct gcccgaggta ctgcatgaaa tccgggtttt ccgggtgggc
     1801 cttttgtagg cgcgccaagg ttttgaccgt gtcgcgggga cggcccagcc gttggtagta
     1861 ggaggccagg cggagcaaca cgccggtctg gggggcgacg cgggccgcct gggccatgaa
     1921 ttgggccgcg tccgcccaac gctcctcctc ctgggccacc aacgcccgcc aaaaattcac
     1981 gaccgggtcc cggggccccg attgggcggc tcgatcaaaa gccgaacggg cccgatccat
     2041 gtcccccgcc tgaaaataaa gctgacccaa ccgggcgttg acctcggggt tatcggcgac
     2101 ccgaaggacg tagtcctcgt agccggccac gccggtgaag tccggcgcgt tcaaccagca
     2161 ccgttcggct atccgtttcc agccgc
//
LOCUS       DAOQLS010000354         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_33257_length_2170_cov_3.338534, whole genome shotgun sequence.
ACCESSION   DAOQLS010000354 DAOQLS010000000
VERSION     DAOQLS010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33257_length_2170_cov_3.338534"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1168)
                     /locus_tag="PK362_07055"
     CDS             complement(239..1168)
                     /locus_tag="PK362_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74622.1"
                     /translation="MMRRFVVASVFCGLALASAGGAGTGASHPDRERLACATKLIRLF
                     PEVPTDHSLDVLNDRLILVLEKDGETLHVFSPYFAGSTKYKFGRDGKTTEEPLPRGLN
                     YDNHVVRVPFSSGDGSPSGFYYVDITASLKVKDRVFNLIYRDAGGPLFIPGDPRPIPS
                     RAYAEYLRGLDLNSKEREKRQKAIPMVQYHSLDIEDRIWDKQNPSYSTASVQLLEEIM
                     ASAEKSIAGEFGHYPAVPTIPADLRKTGKGTRVGLAQDEHARAIVSAEAVLDACRSCV
                     QKSSSSLLMDGWREWGKALDAHAAQRKAHLPKK"
     gene            complement(1235..>2170)
                     /locus_tag="PK362_07060"
     CDS             complement(1235..>2170)
                     /locus_tag="PK362_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74623.1"
                     /translation="GEFRFGAKVTDLAVTEGRVTGVAVNGGEGLESDAVVLAVGNAAR
                     DTFERLHRRGVRLEAKPFSIGARVEHPRAWVDRGLYGSFAGHPQLGAADYKMAHHGDQ
                     GHGAYTFCMCPGGVVVPGASEEGGVVTNGMSEYARAAPNSNSALMVGVGPADYGRGDP
                     LAGVAFQREWERRAFVLGGGGYKAPVQRVGDFLARRPTTRLGPVAPSYLPGVAPANLW
                     DCLPAFVCEGIVEGLAEFNRRMKGFSHPDALLTGVETRSSSPVRILRDDSFQTNLRGL
                     FPAGEGAGYAGGIMSSAVDGLRVAEALLAGAASRE"
BASE COUNT          415 a          722 c          659 g          374 t
ORIGIN      
        1 ccgatatgct ccggtgttaa cggccatcac cagaagggtt cccttttcca ccagaacctc
       61 caaaggtccc cgttcggtcc gcaccacggc gaacgaggcg aatttcccgt acgtgttcaa
      121 atagcgccgt cgggcgcttt cgtattccgc ttcgtttttg ggaaaccggg gaaccggcgc
      181 ctttcgtccg gcagaaaaca atcgcttgaa aagattcaac ggaaggccgg ggcgcgattc
      241 atttcttggg aaggtgggcc ttacgttggg cggcgtgggc gtccagggcc ttgccccatt
      301 cccgccaacc gtccatcaac aaagaggaac tgcttttttg gacgcaggaa cggcaggcgt
      361 ccagcaccgc ttcggccgag acgatcgccc gggcgtgttc gtcctgggcc aagcccacgc
      421 gggttccttt gcccgtcttc cgcaaatcgg cgggaatggt cggaacggcc gggtaatggc
      481 cgaattcccc ggcgatggat ttttcggccg aggccatgat ctcctccaac aattgaaccg
      541 aggccgtcga ataggacggg ttttgtttgt cccaaatccg atcttcgatg tccagggagt
      601 ggtattgaac catgggaatc gccttttgcc gtttttcccg ttccttggaa ttcaggtcca
      661 ggccccgaag gtattcggcg taggcccggg atgggatggg gcgggggtcg cccggaatga
      721 agagggggcc gccggcgtcc cggtaaatca aattaaaaac ccggtccttc accttgaggg
      781 acgcggtgat gtccacgtaa tagaatccgg agggcgaccc gtcgcccgag gagaagggga
      841 cgcgaacgac gtgattgtcg tagttaagcc cccggggaag gggttcctcc gtcgttttgc
      901 cgtcccgtcc gaatttgtat ttcgtcgaac ccgcgaaata aggcgaaaag acgtgaagcg
      961 tctccccgtc tttttccagc accaaaatca atcgatcgtt caatacgtcc agggaatgat
     1021 cggtcggcac ctcgggaaaa aggcggatca gcttggtcgc gcaggcgagg cgctcccggt
     1081 ccgggtggga tgccccggtc ccggcccccc ccgcgctggc cagagccaaa ccgcaaaaaa
     1141 ccgacgccac gacgaaacgg cgcatcataa ttcctcccga tttccaacga tcatgaatca
     1201 ataaaataca aaactttccg tcgatcgggg ccgatcattc gcgcgaagcg gccccggcca
     1261 acagggcttc ggccacccga agcccgtcca ccgccgaaga catgatgccg cccgcgtacc
     1321 cggccccctc gccggcggga aagagaccgc gaaggttcgt ttggaaagag tcgtcccgaa
     1381 gaatgcgcac cggcgaggaa cttcgggttt ccacgccggt caacagggcg tcgggatggg
     1441 aaaacccctt catccgccgg ttgaactccg ccaacccttc cacgatcccc tcgcaaacga
     1501 aggcgggcaa acagtcccac aggttggccg gagcaacccc cggcaaatag gaaggcgcca
     1561 cgggccccag tcgcgtcgtc ggtcgccggg ccaggaagtc ccccacccgt tgaacggggg
     1621 ccttgtagcc gccgcccccc aaaacgaagg cccgccgttc ccattcccgt tgaaaggcga
     1681 ccccggccag gggatcgccc cgcccgtaat cggccgggcc gacccccacc atcagggcgc
     1741 tgttgctgtt gggcgccgcg cgggcatatt cgctcatgcc gttggtgacg acgccgccct
     1801 cctccgaggc gcccggcacc accacgcccc ccgggcacat gcaaaaggtg taggcgccgt
     1861 ggccctggtc gccgtggtgc gccattttgt agtcggcggc ccccagttgg gggtgccccg
     1921 caaaggaccc gtagaggccg cggtccaccc atgcccgggg atgttccacc cgggcgccga
     1981 tggaaaaggg tttcgcctcc aaccgcacgc cgcggcggtg gagccgctcg aaggtgtccc
     2041 gggcggcgtt gccgacggcc aacaccacgg cgtcgctttc caaaccctcc ccgccgttca
     2101 ccgccacgcc ggtcacgcgc ccctccgtca cggccaggtc cgtgaccttg gcgccgaagc
     2161 ggaactcgcc
//
LOCUS       DAOQLS010000355         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_33393_length_2165_cov_3.736967, whole genome shotgun sequence.
ACCESSION   DAOQLS010000355 DAOQLS010000000
VERSION     DAOQLS010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33393_length_2165_cov_3.736967"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PK362_07065"
     CDS             complement(<1..609)
                     /locus_tag="PK362_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC74624.1"
                     /translation="MNTKIAKLVFAAALATIFTGRLFAKETKLLNVSYDPTRELYQDV
                     NKAFADFWKNQTGEIVTVEQSHGGSGKQARAVIDGLDADVVTLALAYDIDAVAEKSGL
                     LPKNWQSRLPHNSAPYTSTIVFLVRKGNPKNIKDWEDLVRPRVSVVTPNPKTSGGARW
                     NYLAAWGYALKKFANDEPKAKDFVTRLFKNVPVLDSGARGATV"
     gene            complement(643..2025)
                     /locus_tag="PK362_07070"
     CDS             complement(643..2025)
                     /locus_tag="PK362_07070"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC74625.1"
                     /translation="MKKQWAVAGAIVLGALGGWNRVTADELADRLSQLEQRQNVLERK
                     YEVDQENAANKAKDTALANAGGKDGFGVKSPDGAFVLKWYGDVQADGRFFLKDGSHGV
                     TSSLTDTFLLRRLRPGVEVTLNKWITGRLQPNFGNGGATLDDAYANLAFRPAAQLRVG
                     RFKPPVGLENLRSSARLTFVERALPTGLVPNYDLGVQLWGELGGGRAAYAVSATNGAA
                     DGANIDGADTTDRKDFAARVFLTPLKNSPNPSLAGLGVGLAGSTGRQVGSASSPGLTA
                     GYKTHGQNTFFAYQGRAVADGNRTRWSPQLTWFSGPWALLGEHVTSRQTVRSTVTLVT
                     ADLIQRSWQVAASYVLTGEDATDKGVKPRHPYEPGNPGWGAWEIAARFAQFTAGRSVF
                     DKVGANTSFASAAASARSARSFSAGLNWYATQSVKWQTDFETTSFDGGATGGNRPVER
                     ALLTRIQLSY"
BASE COUNT          405 a          685 c          643 g          432 t
ORIGIN      
        1 cacggtcgcc ccccgggcgc cggaatccaa caccggaacg tttttaaaca ggcgcgtcac
       61 aaagtctttg gctttgggtt cgtcgttggc gaattttttc aaggcgtacc cccaggccgc
      121 cagataattc caccgggccc ctcccgaagt tttcggattg ggcgtcacga ccgaaacgcg
      181 aggacgaacc aggtcctccc agtctttgat gtttttgggg ttccccttcc gcaccagaaa
      241 cacgatggtc gaggtgtagg gcgcgctgtt gtggggcagg cgggattgcc aatttttcgg
      301 caacaagccg gacttttccg ccaccgcgtc gatgtcgtac gccagggcca gggtgaccac
      361 gtcggcgtcc aggccatcga tgacggcccg ggcctgcttg cccgaccccc cgtgggactg
      421 ctccaccgtc acgatttccc ccgtttgatt tttccagaaa tccgcgaagg ccttgttcac
      481 gtcctggtac agctcccggg tcgggtcgta ggagacgttg agcaactttg tttccttggc
      541 gaataaacgc cccgtgaaaa tcgtggccag agccgccgca aaaacgagtt tggcgatttt
      601 agtgttcatg gaattctcct gttgaaaagt cgaagggaac gatcaatagg acaactgaat
      661 ccgggtcaaa agggcccgtt cgaccggccg attgccgccg gtcgcgccgc cgtcgaagga
      721 cgtggtttcg aaatccgtct gccatttgac gctttgggtg gcataccagt tcagcccggc
      781 ggaaaaagac cgcgccgaac gcgccgaagc ggcggcgctg gcgaaggacg tgttcgctcc
      841 gaccttgtcg aaaacggacc ggcccgcggt gaattgggca aaccgggcgg cgatttccca
      901 ggcgccccat ccggggttgc ccggttcgta gggatggcgg ggtttgacgc ctttgtcggt
      961 ggcgtcttct ccggtcaaga cgtaactcgc cgccacttgc caggaccgct ggatcaaatc
     1021 cgccgtgacc agcgtgaccg tcgagcggac cgtctgtcgg cttgtcacgt gctcgcccag
     1081 cagggcccag ggaccggaaa accacgtcaa ttggggagac cagcgcgtgc ggttcccatc
     1141 cgccaccgcc cgcccttgat aggcaaagaa ggtgttttgg ccgtgggtct tatatccggc
     1201 ggtcaaaccg ggcgacgacg cggacccgac ttgccgtccg gtggatccgg ccaatcccac
     1261 ccccaagccc gccaaggacg gattcggaga attcttcagg ggggtcaaaa agacccgcgc
     1321 cgcgaaatcc tttcgatcgg tcgtgtcggc gccgtcgatg ttggcgccgt cggccgcccc
     1381 gttggtggcg gacacggcgt aggccgcgcg tccgcccccg agctcgcccc acagttggac
     1441 gcccagatcg tagttgggca ccaacccggt gggcaacgcg cgttccacaa aggtcaaccg
     1501 cgcgctggag cgaagatttt ccaatcccac gggcggcttg aagcgaccga cgcgaagttg
     1561 cgcggcggga cggaaggcca aattggcgta ggcatcgtcc aaggtcgccc cgccgtttcc
     1621 gaagttcggc tgaagccgcc ccgtgatcca tttattcagg gtcacttcca ccccgggccg
     1681 aagacgcctc agaagaaagg tgtccgtcaa agaagacgtc actccgtggg acccgtcctt
     1741 cagaaaaaag cgcccgtccg cctgcacgtc gccgtaccat ttcaaaacaa aggcgccgtc
     1801 cggcgattta accccgaatc cgtctttgcc gccggcgttg gccagggcgg tgtccttggc
     1861 cttgttggcg gcgttttcct gatcgacttc gtatttccgc tccagaacgt tctgacgttg
     1921 ctccaattgg ctcaaccgat ccgccaattc atccgcggtc acccgattcc atccgcccaa
     1981 agcgcccaaa acaatcgctc cggccactgc ccattgtttc ttcatcgtat tctccttcgt
     2041 tttattggcc gggtctcaaa aaaagacccg tgccgagtgt tgcggcacgg gtcacaaagg
     2101 cttttgtgga ctcgaccgct ttagcgccct gttcactccg aagagtcggc cggggcaagt
     2161 tggaa
//
LOCUS       DAOQLS010000356         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_33537_length_2160_cov_3.682660, whole genome shotgun sequence.
ACCESSION   DAOQLS010000356 DAOQLS010000000
VERSION     DAOQLS010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33537_length_2160_cov_3.682660"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PK362_07075"
     CDS             complement(<1..874)
                     /locus_tag="PK362_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNC74626.1"
                     /translation="MKKNLRVAGCDLGKSTAKFVVLRLDETGPVVESRESRPHDGRPM
                     AIFQEWYRAAGALDVLGATGVHAGELVSPVLHGLPEEACLARALDAFGPTGAVNLVSV
                     GARGYAVMSRDADGRVQFVENEKCSSGTGETMVKNARRFGLSIEEADRLARGARESIP
                     ITARCSVFAKSELTHFGNQGKPADALFRGYFESIAHFVGALLARVRVAGPVWFIGGGA
                     RLGSLSAAMGRALGTAVDVPAEALYLEALGAALLAADQWAHEAPPTLPPDPATLIVKA
                     GISSRYFGVKFNKTR"
     gene            complement(975..1604)
                     /locus_tag="PK362_07080"
     CDS             complement(975..1604)
                     /locus_tag="PK362_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74627.1"
                     /translation="MEVAPRPARSFKERYPSPVLGEFGPSPVQGLAFFKVYDKSQMAE
                     DPLIPALWIRTPDGQMLETDLDGYRRALSAVRWAPASAKEALVFARFYATAKTGLNHR
                     RVVNGKESAAEVGIKSALDLKKIGATPPSVEKSEQTVCRPRGETGTDRLLIPVWVVRL
                     CVADVQPKAWGMPGHETIYRLTVTVGDGIFDVDQETLWGGNEIKSVPIK"
     gene            complement(1749..>2160)
                     /locus_tag="PK362_07085"
     CDS             complement(1749..>2160)
                     /locus_tag="PK362_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC74628.1"
                     /translation="AFTLIELMLVVAIIGLLAAIAIPKFANMVVKAKEAAVIGKLGAL
                     RSAISIYYADNEGRFPQQVDLRLLNGKYMEEIPRISIPTVPAHAFNNFSTMDPIGDWF
                     DYAYNYNAPNLSGNGKVNVHCTHTDSKGTTWSLY"
BASE COUNT          395 a          684 c          670 g          411 t
ORIGIN      
        1 ccctggtttt attgaatttg accccgaaat atctggacga tatccccgcc ttgacgatca
       61 acgtcgcggg gtccgggggg agggtcggcg gggcttcgtg ggcccattgg tcggccgcga
      121 gaagcgcggc ccccagagcc tccaagtaga gggcttccgc gggaacgtcg acggccgtgc
      181 ccagggcccg tcccatggcg gcgcttaagg accccaggcg cgcgccgccg ccgatgaacc
      241 acacggggcc cgccacgcgg acccgggcca gcagggcgcc gacgaaatgg gcgatggatt
      301 cgaagtaacc ccggaagaga gcgtccgcgg gtttcccttg gttgccgaaa tgggtcagct
      361 cgcttttggc gaaaacggag caacgggcgg tgatggggat ggattctcgc gctccccggg
      421 ccaggcgatc ggcctcctcg atggaaaggc cgaaacggcg ggcgtttttg accatggttt
      481 cgcccgtgcc ggaagagcat ttttcgtttt cgacgaattg aacgcgtccg tcggcgtccc
      541 gggacatgac cgcgtagccc cgggcgccga cgctcaccag gttgacggcc cccgtcggtc
      601 caaaggcgtc gagggcccgg gccaggcagg cctcctcggg cagaccgtgg agaacggggg
      661 aaaccagctc ccccgcgtgg acccccgtgg cgcccagcac gtcgagggcg ccggccgctc
      721 ggtaccattc ctggaaaatc gccatgggcc gcccgtcgtg ggggcggctc tcccgggatt
      781 ccacgaccgg acccgtttcg tccagccgaa gcaccacgaa cttggccgtg gatttcccga
      841 gatcgcaccc ggccacgcga agattttttt tcaagcctcg ccctccgacc ccggacgctc
      901 cgggaaccca tggttcacat ggctcgtccc tgccgaaaac ctgtgaggcc ctggctcaac
      961 ggggccccaa ctttttactt tatcggcacg ctttttattt cgttcccgcc ccagagagtt
     1021 tcctggtcga catcgaaaat gccgtcgccc accgtgaccg tgaggcgata gatcgtctcg
     1081 tggccgggca ttccccaggc tttgggctga acgtccgcga cgcacagccg aaccacccac
     1141 accggaatca acagacggtc ggtccccgtc tccccccggg gacggcaaac ggtttgttcc
     1201 gatttttcca cggacggcgg cgtcgccccg atttttttca ggtcgagggc gcttttgatt
     1261 ccgacctccg ccgcggactc cttcccgttg accacccggc gatggttcaa accggttttg
     1321 gccgtggcgt aaaaccgggc gaagaccagg gcctccttcg cagaggccgg agcccaacgg
     1381 accgcgctca aggcccggcg gtacccgtcg aggtccgtct ccagcatttg tccgtcgggg
     1441 gttcggatcc acagggcggg gatcaggggg tcctcggcca tttgtgattt atcgtaaacc
     1501 ttgaagaaag cgagcccctg aaccggcgag gggccgaact cccccaacac gggacttgga
     1561 taacgctcct taaaagaacg ggcgggacgc ggtgcgactt ccatgggtgg ggcctccgaa
     1621 tcgggcgggc gcggggttgc gttccccgcc gggacgatca cgacgggtcc cgccattaat
     1681 ataaaaattc gtgccatcgt tttcattgaa ttccaatcgg cgcggggccc accgatgctt
     1741 tcttcaccct agtacaaact ccaggtggtt cctttactat cggtgtgcgt gcagtggaca
     1801 ttgaccttgc cgttgcccga caagttggga gcgttgtaat tgtaggcata atcgaaccaa
     1861 tccccgatcg gatccatggt ggaaaaattg ttaaacgcgt gggcgggaac cgtggggatg
     1921 gatattcgcg ggatctcctc catgtatttc ccattgagca aacgcaaatc aacttgctgg
     1981 ggaaaccggc cttcgttgtc cgcgtagtaa atgctgatgg ccgaccgaag cgcccccaat
     2041 ttcccgatca ccgcggcctc tttggctttc accaccatgt tggcaaattt gggaatggcg
     2101 atggccgcga ggaggccaat gatggccacc acgagcatca gttcaatcag ggtgaacgcc
//
LOCUS       DAOQLS010000357         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_33604_length_2158_cov_3.881598, whole genome shotgun sequence.
ACCESSION   DAOQLS010000357 DAOQLS010000000
VERSION     DAOQLS010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33604_length_2158_cov_3.881598"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /gene="hpnE"
                     /locus_tag="PK362_07090"
     CDS             <1..1241
                     /gene="hpnE"
                     /locus_tag="PK362_07090"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HNC74629.1"
                     /translation="YRDGVTRQDVDNGQHLFLGAYRDTRAFLRRLKVENRLRWLPGAS
                     TEFYARSGKKSVLNPGRLPGALGFLRGVLGFGALTWRDKASLLYGLTRLRWDRSDNHG
                     GRTVAAWLKGLRQTPGALRAFWDPLCYATLNERPDRAAADALVSVLREGFFKSVEDRA
                     LGYSTVPLGRLWATELPAYLKNQGGDVAAKIRATGFVVEDGRVRALEIEGGTPVPADV
                     FVSAVPLRAARALMGPRVPALSDLPSDASPIAAVNFWFSRPPFAAPWVGFLDLDIQWG
                     FNREVLWGRGAEGQVSLVLSAGRDHVARPSDQLIALALADLRRAFPAFKEEPTHATVT
                     WEKEATPAPTPGFWRGRPGVTTPLANLFLAGDWIDAGLPPTIEAACRSGHRAAAFALR
                     WLDGQKEDECLKPYCSTAMA"
     gene            1205..1900
                     /locus_tag="PK362_07095"
     CDS             1205..1900
                     /locus_tag="PK362_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNC74630.1"
                     /translation="MLKAVLFDCDGVIADSEHLHFSLFQKVLREINLPLTQADYLEKY
                     LAMDDKGCFRAVAAAHGRSLSDTELSALIEKKTALYKKTATENLVILPGVVEFVMAVS
                     KKFPLAMASGALRDEVKLMIHAAGIAHYFDAVVAAEDVVRGKPAPDAYLKALEEINKK
                     YPDRRIQPAECLVVEDSKHGLLSARAAGMKCVAVTTSYSAEELSAADRVVPVLTAVRV
                     KDLESLFLSATPQ"
     gene            1897..>2158
                     /locus_tag="PK362_07100"
     CDS             1897..>2158
                     /locus_tag="PK362_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74631.1"
                     /translation="MTPPTPRARLLQLLDRDDAGVDLAEAALLVAGDEYPGLDPRPYL
                     DRLDGFSDRVAARLRGGPDPVAAIAALNAVLFEEERFGPNQKD"
BASE COUNT          353 a          657 c          719 g          429 t
ORIGIN      
        1 cttatcggga cggcgtgacc cgccaggacg ttgacaacgg ccaacacttg tttttggggg
       61 cataccggga cacccgggct ttcctgcgcc gtctgaaggt tgaaaatcgc ctgcggtggc
      121 tcccgggggc ttccaccgaa ttttacgccc ggtcgggcaa aaaatccgtt ttgaaccccg
      181 gtcgacttcc cggcgcgttg ggattcttgc ggggcgtcct gggttttggg gcgttgacgt
      241 ggcgggacaa agcgtctctg ctctacggtt tgacccgact gcgatgggat cggtccgaca
      301 accacggcgg gaggacggtg gcggcgtggt tgaaaggcct gcgtcaaacc ccgggcgcct
      361 tgcgggcctt ttgggacccc ctgtgctacg ccacgttgaa cgagcgcccg gaccgggccg
      421 cggccgacgc cctggtgtcc gtccttcgcg aagggttttt taaatccgtt gaggaccgcg
      481 ctctcggtta ttcaaccgtt ccgctgggac gcctttgggc gacggaattg ccggcgtatt
      541 tgaaaaacca gggcggcgac gtggcggcca agatccgggc cacgggattc gtcgtcgaag
      601 acgggcgggt tcgcgcgttg gaaattgaag gagggacccc ggtgccggcc gacgtttttg
      661 tctcggcggt gccccttcgg gccgctcggg cgctgatggg gccccgggtg ccggccctgt
      721 cggatttgcc gagcgacgcg tcgcccatcg ccgccgtcaa tttttggttt tcccggccgc
      781 cctttgcggc cccctgggtc ggattcttgg acctggacat ccaatggggt ttcaaccggg
      841 aagttctttg ggggcgcggc gcggaggggc aggtgtcgtt ggtcctcagc gcggggcgcg
      901 atcacgtggc gcgacccagc gatcagctca tcgctctggc cctggcggac cttcgccggg
      961 cctttccggc ttttaaagaa gagccgaccc acgccacggt gacctgggaa aaggaagcca
     1021 cgcccgcccc gacgccgggg ttttggcgcg gacggccggg ggtcacgacg cccctcgcca
     1081 atcttttcct ggcgggcgat tggatcgacg ccggattgcc cccgaccatc gaggccgctt
     1141 gccgaagcgg tcatcgggcc gccgctttcg ccttgcgttg gttggacggc caaaaggagg
     1201 acgaatgctt aaagccgtat tgttcgactg cgatggcgta atcgccgatt cggagcacct
     1261 gcatttttcg ctgtttcaga aagtccttcg cgaaatcaat ctgccgttga cccaggcgga
     1321 ttatttggaa aaatacctcg ccatggacga caagggatgc ttccgcgccg tggccgccgc
     1381 ccacgggcgg tctttgtcgg acacggaatt gtcggccctt atcgaaaaga agacggcgct
     1441 ttacaagaaa acggccaccg agaacctggt catcctcccg ggggtggtcg aatttgtgat
     1501 ggccgtgtct aagaaatttc cattggccat ggcctcgggc gctttgcggg acgaagtgaa
     1561 attgatgatc cacgccgccg gcatcgccca ctacttcgac gcggtggttg ccgcggagga
     1621 cgtggtccgg ggaaagccgg cccccgacgc ctatttgaag gcgttggagg aaatcaacaa
     1681 gaaatacccg gaccgccgca ttcaacccgc ggaatgcctg gtcgttgaag attcgaaaca
     1741 cggattgctt tccgcccggg ccgccggcat gaaatgcgtg gcggtcacga cgtcctattc
     1801 cgccgaggaa ttgagcgcgg ccgaccgggt ggttccggtc ctgaccgccg tccgtgtgaa
     1861 agacctcgaa agcctctttc tatccgcgac cccgcaatga cgccgccgac tccccgcgcc
     1921 cgcctgctcc agttgctgga ccgtgacgac gcgggggtgg acttggccga ggcggctctt
     1981 ttggtggcgg gggacgaata tccgggtctg gacccgcgac cctatttgga tcgactggac
     2041 ggtttttccg accgggtggc ggcccgcctt cggggagggc ccgatcccgt ggccgccatc
     2101 gcggccctga acgccgtgtt gttcgaggaa gagcgctttg ggccgaacca aaaagact
//
LOCUS       DAOQLS010000358         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_33673_length_2156_cov_3.854831, whole genome shotgun sequence.
ACCESSION   DAOQLS010000358 DAOQLS010000000
VERSION     DAOQLS010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33673_length_2156_cov_3.854831"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK362_07105"
     CDS             complement(<1..183)
                     /locus_tag="PK362_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74632.1"
                     /translation="MSLRSPGWIYAAAVLALGLETAGAVWIKTAPPPVSPDRVLTDLC
                     TPRTRHHEPSISRMDIR"
     gene            264..1535
                     /locus_tag="PK362_07110"
     CDS             264..1535
                     /locus_tag="PK362_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74633.1"
                     /translation="MQKVLIIDDFDPFRGMARRVLSRLGYRVLEAPDGRQGLEIFGRE
                     QPDVVITDLRMPGLDGSQVLAEIKQKSPDTEVVVLTGFATERTEHDLLKQGAFVCLRK
                     PFNLEQIVKVLDRIREKGGVGDPRPVALVHVEGDRRRLFLDRVLRKNGLEPRFPESPE
                     EARRLLRSGELDFLLWEVSNASLSDAKALVEARESLADLAVVPLLVDAGPDVEAFREA
                     LGVAPADCLKEPLDEESLSALFRRLLLRLLTRRNELYKQQKAAAVGTVSLQFGAGGEL
                     VADFRGRGRQFLYTLTSALKDFPWPFMVLSAAWDVVYVSDRLKALCGFAPEKIDLHLW
                     DALNRKGLSLPPLDDLYRHLARTIRRETGGADTVLSPEGGGLGLLALRALPETQGDGG
                     LVALLFPDAPGPAVIDVRPAGDRPALPPPLL"
     gene            1654..>2156
                     /locus_tag="PK362_07115"
     CDS             1654..>2156
                     /locus_tag="PK362_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74634.1"
                     /translation="MRIANNVSALNSWRQLSRNHAMFDSSLEKLSSGLRINKAGDDPA
                     GLVLSEQLRAQITGLKQAVRNTQDAASMIQTGEGAMQEMHSILNEIRSLVLHAKNSGV
                     VDATAIAADQSQVDEAVAALNKIANNTTFGGKNLLNGSLSNRGVVLDATAVSSVTAGT
                     SAPSGTNL"
BASE COUNT          363 a          684 c          720 g          389 t
ORIGIN      
        1 gcgtatatcc atccgggaga tcgaaggctc atggtggcgt gttcgcgggg tgcaaaggtc
       61 ggtgagaacg cggtccgggg aaacgggggg cggcgccgtt ttgatccaca cggcgccggc
      121 cgtttccaat ccgagggcca gaaccgcggc cgcgtatatc catccgggag atcgaaggct
      181 catggtggcg tgttcgcggg gtgcaagggg cgtgccgggg tcaaggcccc ccccgggcgg
      241 tcgataatga aaaggaggcc cccatgcaaa aggttctgat catcgacgac ttcgatccct
      301 tccgcgggat ggcccggcgg gtgttgtccc gtttggggta tcgggtgttg gaggccccgg
      361 acgggcggca ggggttggaa atattcggtc gggaacaacc cgacgtcgtc atcaccgacc
      421 tgcgaatgcc cgggctggac gggtcccagg tgttggcgga gatcaaacaa aaatcgcccg
      481 acacggaggt cgtggtgttg acgggcttcg ccacggaacg gacggagcac gatttgctga
      541 agcagggggc ctttgtctgc ctccgcaagc ccttcaacct ggagcagatc gtgaaggtgc
      601 tcgaccgcat tcgggaaaaa ggaggcgtcg gggatccgcg gcccgtggcc ctggtccacg
      661 tggaggggga tcggcggcgc ctgttcctgg accgggtgct tcgcaaaaac ggcttggagc
      721 cccgttttcc cgaatccccg gaggaagccc gccgattgct tcgttcgggc gagttggatt
      781 tccttctttg ggaggtgtcg aacgcttccc tgtcggacgc caaggccttg gtggaggccc
      841 gggaatccct ggccgatttg gccgtcgtgc ccctgttggt cgacgcgggg cccgacgtgg
      901 aggcgttccg ggaagccctg ggggtggcgc cggccgactg tttgaaggaa cccctggacg
      961 aggaaagcct ttcggccctt tttcgacggc ttcttcttcg attgttgacg cgtcggaacg
     1021 agctttacaa gcagcaaaag gccgcggcgg tggggaccgt gtcgctccag ttcggcgcgg
     1081 ggggggaatt ggtcgcggat tttcgcggtc ggggccggca attcctctac accctcactt
     1141 ccgctttaaa ggacttcccc tggcctttca tggtgttgag cgccgcctgg gacgtggtct
     1201 acgtgagcga ccggttgaag gccctgtgcg gcttcgcccc cgaaaaaatt gatttgcacc
     1261 tctgggacgc cctgaaccgc aaggggcttt ccctgccccc cctcgacgat ctttaccgcc
     1321 atctggcccg aaccatccgc cgggaaaccg gcggcgccga caccgtcctg tcccccgaag
     1381 ggggcggttt gggcctcctg gcccttcggg ccctgcccga aacccagggc gacgggggcc
     1441 tggtggctct gctgtttccg gacgcccccg ggcccgcggt gatcgacgtg cggccggccg
     1501 gcgaccgccc cgccctcccg ccgcccctcc tctagttttc cgaccccggg tcgataatct
     1561 ttttgcgggt ctttcgggat ccgcgcgccg gccctcgggc cggcgcgacc gggaaaggaa
     1621 tcccggcaac ctcacacacg gaaggagtta atcatgcgca tcgccaacaa cgtttccgcc
     1681 ttgaactcgt ggcggcaact gtctcggaac cacgccatgt tcgacagcag cttggagaaa
     1741 ctgtccagcg gtctccgcat caacaaagcc ggggacgacc cggccgggtt ggtgttgtcc
     1801 gaacagctgc gggcccagat caccggtctc aaacaagccg tccgcaacac gcaggacgcc
     1861 gcctcgatga ttcaaaccgg ggaaggcgcc atgcaggaaa tgcactccat cttgaacgaa
     1921 atccggtcct tggtcctgca cgccaagaac tcgggcgtcg tggacgcgac cgcgatcgcg
     1981 gccgaccaat ctcaagtgga cgaagccgtc gcggccttga acaagatcgc caacaacacg
     2041 accttcggcg gcaagaacct gctcaacggg tccctgtcga accgcggcgt ggtgctggac
     2101 gccacggcgg tctcctccgt cacggcgggc acctcggcgc cctccgggac gaacct
//
LOCUS       DAOQLS010000359         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_34017_length_2144_cov_3.767831, whole genome shotgun sequence.
ACCESSION   DAOQLS010000359 DAOQLS010000000
VERSION     DAOQLS010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34017_length_2144_cov_3.767831"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PK362_07120"
     CDS             <1..585
                     /locus_tag="PK362_07120"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNC74635.1"
                     /translation="RRHIQDKDVSLLRKEKGLRLNLEMAATEEMVRIARVVRPDSVCL
                     VPEKRQELTTEGGLNVERELSRLREIVRKIKGKGIVVSLFIDPDPAQLQAAKDAGADW
                     VELHTGAYAGAAGAARGAELAKIEKAGALALGLGLGLNAGHGLDYDNVGPVARLPRMQ
                     ELNIGFSIIARALFVGLETAVREMKSLVDRARPA"
     gene            595..>2144
                     /gene="glmS"
                     /locus_tag="PK362_07125"
     CDS             595..>2144
                     /gene="glmS"
                     /locus_tag="PK362_07125"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNC74636.1"
                     /translation="MCGIVGYIGKNEASDVLLDGLRRLEYRGYDSAGMVVLDGGRLDI
                     RRSVGKLMNLEALLKKEPLRGGAGVGHTRWATHGRPSEENAHPHRDATGRVVVVHNGI
                     IENYTTLKAGLVARGVRFASQTDTEVVAHLVGEAIARRRSKAALSADQFVEAVREALS
                     EVKGTYALGIVSADCPGVVLGARRECPLLIGVGEGENFLASDAPAILSRTREAVFLQD
                     GDMAVLTADAYRVISIDTGRPVERKKVHLPWDPLQAEKAGYRHFMLKEIHEQPRALED
                     TLRGRLSLDDTGVHLDTLKLSEDQIRGFKKMTIVACGTSFHAGLVARYLIEKWVKAPC
                     DVEIASEYRYRDPLIGPGDLVVAISQSGETADTIAALRHAKEKGARTLAVCNVVGSTL
                     YREAHGKILTHCGPEIGVASTKAFTSQLVVLYLLAVYGGLLRGTLPRTEVEKILQDLL
                     HLPRWVGDALKEDPGVLALAKKFSHSHNFLYLGRNLNYPIALEGALKLKEISYIHAEG
                     YPSGELKHG"
BASE COUNT          402 a          680 c          702 g          360 t
ORIGIN      
        1 cggcgccaca tccaagacaa ggacgtttcc ctgcttcgca aggaaaaggg tctccggctt
       61 aatttggaaa tggcggccac ggaagaaatg gtccggatcg cccgggtggt ccgcccggac
      121 agcgtgtgcc tggtccccga aaaacgccag gagttgacca ccgagggcgg gttgaacgtg
      181 gagcgggaac tttcgcggct tcgcgaaatt gttcgaaaaa tcaaaggcaa agggatcgtg
      241 gtcagcctgt tcatcgaccc ggatccggcg caacttcagg cggccaagga cgccggcgcg
      301 gattgggtgg agctccacac gggggcctac gccggggcgg ccggagcggc ccggggcgcc
      361 gagctcgcga aaattgaaaa ggccggagcg ttggctctcg ggctcggcct cgggctgaac
      421 gccggccacg gcctggatta cgacaacgtc ggcccggtgg cccgcctgcc tcggatgcag
      481 gaattgaaca tcggattttc catcattgcc cgggcgcttt ttgtcggcct cgaaacggcc
      541 gtccgggaga tgaaatcgct cgtcgaccgg gcccggcccg cttaggagtt ccttatgtgc
      601 ggcatcgtcg gttacatcgg caagaacgaa gcgtcggacg tcctgctgga cggtttgcgg
      661 cggttggaat accgcggcta cgactcggcg ggcatggtcg ttttggacgg cggtcgtctg
      721 gacatccgcc ggagcgtcgg caaactgatg aatctcgagg cgctcttgaa gaaggagccc
      781 ctgcgcggcg gcgccggcgt cggccacacc cggtgggcca cccacggccg accctccgag
      841 gaaaacgccc acccccatcg ggacgccacg ggccgggtgg tcgtggtcca caacggcatc
      901 attgaaaatt acaccaccct caaagcgggc ctggtggccc ggggggtccg gttcgcgtcc
      961 cagaccgaca cggaagtggt cgcgcacttg gtgggagaag ccatcgcccg ccgacggtcc
     1021 aaagccgcgc tgtcggcgga ccaattcgtc gaagccgtcc gggaggcgtt gtccgaggtc
     1081 aagggcacct acgctctggg catcgtttcg gcggactgcc ccggcgtggt cctgggcgct
     1141 cgacgggaat gccccctgtt gatcggcgtc ggcgaggggg agaatttcct cgcttcggac
     1201 gcccccgcaa tcctgtcccg gacccgggaa gccgttttcc tccaggacgg ggacatggcc
     1261 gtgttgaccg cggacgccta ccgggtgatt tccatcgaca ccggccgccc ggtggaacgc
     1321 aagaaagtcc atctgccctg ggacccgctc caggccgaga aggccggcta ccgccatttc
     1381 atgttgaagg aaatccacga acagccccgg gcgttggaag acaccctccg cgggcgactg
     1441 agcttggacg acaccggcgt ccacctcgac acgctgaagc tttccgaaga ccaaatccgg
     1501 ggatttaaaa aaatgacgat cgtggcctgc ggaacctcct tccacgcggg cttggtcgcg
     1561 cgttacctca tcgaaaaatg ggtcaaggcg ccctgcgacg tggaaatcgc ctccgaatac
     1621 cgctaccggg acccgctgat cggcccgggc gacctggtgg tggccatctc ccaatccggg
     1681 gaaacggccg acaccatcgc ggcgttgcgc cacgccaagg agaagggggc ccgaaccctg
     1741 gccgtctgca acgtcgtcgg gtccactctt taccgggagg cccacggcaa aatcctgacc
     1801 cactgcgggc ccgagatcgg ggtggccagc accaaggcgt tcacgtccca actcgtggtt
     1861 ctgtatttgc tcgccgtgta cgggggactc ctgcgcggga cgctcccccg aacggaggtg
     1921 gaaaagattc ttcaagacct cctgcatttg ccccggtggg tgggcgacgc gctcaaggaa
     1981 gatcccggtg ttttggcgct ggccaagaag ttttcccaca gccacaattt cctttacctg
     2041 gggcggaacc tgaattaccc catcgcgctc gagggggcgc tcaagctgaa ggaaatttcc
     2101 tacatccacg ccgaagggta tccgtcgggc gaactcaagc acgg
//
LOCUS       DAOQLS010000360         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_34098_length_2141_cov_4.024928, whole genome shotgun sequence.
ACCESSION   DAOQLS010000360 DAOQLS010000000
VERSION     DAOQLS010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34098_length_2141_cov_4.024928"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PK362_07130"
     CDS             complement(<1..752)
                     /locus_tag="PK362_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74637.1"
                     /translation="MVRWRRVLFFLLAGSVASVRAHEVGYRVLRVPVGGKSIAVAVWY
                     PTEASSRPIVYGTARVAGRAAADAPLKPGRWPLVLFAHGYSGSGTGSAAIAEGVARAG
                     YVWAAPDFSDVVSEVRIDGPVTGNLRDALRNLKDKPPSMENHGYRVEEARAVLRVLRD
                     DPKWSIDPERIALAGHSLGGWTVVRLALDDPRPRPLVLYSMGELNYLFKNARIVPGRD
                     LARLRGPAYLTYGERERTALRGRSPNTLYVLA"
     gene            complement(869..1141)
                     /locus_tag="PK362_07135"
     CDS             complement(869..1141)
                     /locus_tag="PK362_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC74638.1"
                     /translation="MPPPTPTRKSRTIAEITPDRCTGCEACMAVMPHDDCIGKASDDP
                     TLPTTLIVCEVNVDRCTGCTLCMKICPWDAIAMVPRPGYEAPKPAS"
     gene            1214..1351
                     /gene="rpmH"
                     /locus_tag="PK362_07140"
     CDS             1214..1351
                     /gene="rpmH"
                     /locus_tag="PK362_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNC74639.1"
                     /translation="MPKRTYQPHKRRRAKTIGFRARMATPGGRRVIKRRRQKGRWKLT
                     K"
     gene            1425..1790
                     /gene="rnpA"
                     /locus_tag="PK362_07145"
     CDS             1425..1790
                     /gene="rnpA"
                     /locus_tag="PK362_07145"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNC74640.1"
                     /translation="MNFSFRRHERLTRSRDFDRARRKGRRRVGRRLVLWAYRREETPA
                     RATRLGVVVGRRHGGAVQRNRFKRRVRDIFRTEKNRWPRGWDLVVTPKTGGPAFPPPH
                     DDLRSDFLSLMERSAHGDK"
     gene            1787..2005
                     /gene="yidD"
                     /locus_tag="PK362_07150"
     CDS             1787..2005
                     /gene="yidD"
                     /locus_tag="PK362_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015122968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNC74641.1"
                     /translation="MKSFLQFLIRAYQAAGFLWKPRCRFLPTCSHYALEALERHGAAR
                     GLALTLGRLARCHPLGGRGVDLVPPLPQ"
BASE COUNT          370 a          725 c          698 g          348 t
ORIGIN      
        1 tgggccaaaa cgtaaagcgt gttgggcgac cggccccgca gggccgtccg ctcgcgttcg
       61 ccgtaggtaa gataggcggg cccccgcagc cgggccaagt cccggccggg cacgatgcgg
      121 gcgtttttaa acaaataatt caattcgccc atggaataga gaacgagggg ccggggccga
      181 ggatcgtcca acgccaaccg aacgacggtc cacccgccga gggaatgccc cgccagggcg
      241 atccgttcgg ggtcgatgga ccatttcgga tcgtcccgca acacccgcaa gacggcccgg
      301 gcctcttcca cgcgataacc gtggttttcc atggagggcg gtttgtcctt taaattccga
      361 agcgcgtccc gaaggttccc cgtgaccggc ccgtcgatgc ggacctcgga cacaacgtcg
      421 ctaaaatccg gggcggccca cacgtacccc gcccgggcca cgccttcggc gatggccgcc
      481 gagccggtcc cgcttccgga atagccgtgg gcaaagagaa ccaggggcca gcggcccggt
      541 ttcaacgggg cgtcggcggc cgcgcggccc gccacgcggg cggtgccgta aacgatcggc
      601 cgcgacgacg cctcggtggg ataccaaacg gccacggcga tggatttacc gccgacggga
      661 acccgcagga cccggtaccc cacctcgtgg gcgcgcacgg aagcgaccga ccccgcgagg
      721 agaaaaaaga ggacccgccg ccatcgaacc atgagagatg cctttcggaa ttgaaggtgg
      781 ggggagaggt tatcgcgaac cgcccccgct tgtgttggac gacggcgatg ggacgaaggt
      841 gtcgccgaaa ctccgccgtc cgaggaaatc aggaggcggg tttcggggct tcgtacccgg
      901 gacggggcac catggcgatg gcgtcccagg ggcaaatctt catgcagagg gtgcaaccgg
      961 tgcaacggtc gacgttcact tcgcagacga tcaacgtggt cggaagcgtc gggtcgtccg
     1021 acgccttccc gatgcagtca tcgtggggca tgacggccat gcaggcctcg cacccggtgc
     1081 agcggtcggg ggttatttcc gcgatggtgc gcgatttccg cgttggggtg ggggggggca
     1141 tgggccacag tttactcgcg gagcagcgcc gcgtcaattg acacccggcc tccgtcaagc
     1201 taaaatgcct cccatgccta agagaactta tcaaccccac aagcgtcggc gcgccaaaac
     1261 catcggcttt cgcgcgcgga tggccacgcc cgggggacgg cgcgtcatca aacgccgtcg
     1321 ccaaaagggc cgctggaaac tcacgaagta accccccgcc cggttttccc ccgtcggacc
     1381 gccgatcgtc ggcgcccccc gctgtttgaa aatcggacgt tcgcgtgaat ttttccttcc
     1441 ggcgccacga gcgtctcacc cgttcccggg attttgaccg cgcccggcgg aagggtcgcc
     1501 gccgcgtggg ccgtcgcctc gtgctgtggg cctaccgccg ggaggaaacc ccggcccgcg
     1561 ccacgcgcct cggcgtcgtg gtcggccgcc gccacggcgg ggccgtccaa cgcaaccgct
     1621 ttaaacgccg cgtgcgggat atatttcgaa cggagaaaaa ccgctggccc cggggctggg
     1681 acctggtggt caccccgaaa acgggcggcc ccgcttttcc ccctccccac gacgatttgc
     1741 gctcggactt tctgtcgctg atggaacggt ccgcgcacgg agacaaatga aatcgtttct
     1801 gcagtttttg atccgcgcct accaggccgc gggctttctc tggaagcccc gctgccggtt
     1861 tttgcccacc tgttcccact acgccctcga ggcgttggaa cggcacggcg cggcgcgcgg
     1921 actggccctg accctgggac gtctcgcccg gtgccacccc ctgggtggcc gcggcgtcga
     1981 tttggtcccc cccctccccc aatgaaaaaa aactacgtgt tggctttcgt tttgtccatg
     2041 tcgattctgg tcttctggga agtgttcgtc ctgaagcccc gacgtcaggc cgccgaaacg
     2101 gccgcggtcc ccgcggcggc gacggtcccg tcggccaacg g
//
LOCUS       DAOQLS010000361         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_34336_length_2132_cov_3.940780, whole genome shotgun sequence.
ACCESSION   DAOQLS010000361 DAOQLS010000000
VERSION     DAOQLS010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34336_length_2132_cov_3.940780"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1526
                     /locus_tag="PK362_07155"
     CDS             201..1526
                     /locus_tag="PK362_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNC74642.1"
                     /translation="MTDSLPSAPRRLGLWDASALVVGCIIGAGIFRLSDSVARASPSV
                     FLFLAAWVLGGLLSLCGALVWAELATRFPRNGGEYVFLSNGYGRAWGFVYGWTRLFVS
                     RTGTLAILAFVFAEHAARVFGAGPGAVKPVATAAVLGLTALNILGVRFGKSVQNLFTV
                     LKLLALFGIIAVGLLAGGGSPVHLTPFWPAEHTSILSSLGLALIPVLWTYGGWYEAAY
                     VAGEVRDPNINLPRAIVGGLLATTGIYLLINLVYVLYLPLDEMRVTDLVAAGVMDKIW
                     PRGGGSIVAAMVMVSTFGALNGFIFSSGRLLSALGQDHALFKKMGVVSARTQTPAAAL
                     AANAVLALVLVWTGTLDKIVTYTEVVIYLFFAATGVSLFVFRRREGAAPGGYRVWGYP
                     WTPLAFILLNVAIALNGVAEEPAVALAGIGVAALGFPLYWASRRLGPKT"
     gene            complement(1523..>2132)
                     /locus_tag="PK362_07160"
     CDS             complement(1523..>2132)
                     /locus_tag="PK362_07160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNC74643.1"
                     /translation="PPPGPKDGSLEGLKIVVDAGHSWDIGSRGPTGLLEKDANLAIAK
                     CLERKLRSEKADVVMLRYGSENVNLYDRPKLAWAARGDVLISVHNNALPDGANPFERN
                     GYGVYYYHPQSEDLATEIHEAYGDIFGRAQAFRSTHLRDDGLHYGNLALARTSQMPAV
                     LTESAYVIWPPEEELLKTETFQCDCAEAMFRGLKRFVKKMRD"
BASE COUNT          312 a          683 c          665 g          472 t
ORIGIN      
        1 ctgcaggctg cgcttaacga aaacgatttt ccgtttggcg cgggggttgt gaaagggatc
       61 gtgcgtggga tgcgcgtgag ccattggtga aaacttaaca gacattgtac cgtttgtcaa
      121 ccccgccgcc cgacaaagtt tatttttgat atcgcccgcc gagattgatc ccgggccgaa
      181 tcaatgttat ccttcggccc gtgacggact ccctcccttc cgctccccgg cgactcggcc
      241 tctgggacgc ctcggccctc gtggtcggct gcatcatcgg cgcgggcatt ttccggctct
      301 ccgattccgt cgcccgggcc tcgccgtcgg tgttcctctt cctggccgct tgggtgctgg
      361 gcggcctcct gtcgctgtgc ggcgccctcg tgtgggccga gctggccaca cgctttcccc
      421 gaaacggggg cgagtatgtg tttttgtcca acgggtacgg gcgcgcctgg gggttcgtct
      481 acgggtggac ccggctcttc gtcagccgca cgggcaccct cgccatcctg gcctttgtct
      541 tcgcggagca cgccgcccgg gttttcgggg ccgggcccgg ggcggtcaaa cccgtggcga
      601 ccgccgccgt tctggggctc acagccctca acatcctcgg tgtccggttc ggaaaatccg
      661 tccaaaactt gtttacggtt ctgaaattgc tcgccctttt cggcatcatc gccgtgggcc
      721 tattggccgg cgggggatcg ccggtccacc tgaccccctt ctggcccgcg gaacacacgt
      781 ccatcctctc ctccctcggg ctggccctga ttccggtgct ttggacctac ggggggtggt
      841 acgaggcggc ctacgtggcg ggcgaagtgc gggacccgaa catcaacctc ccccgggcca
      901 ttgtcggggg cctgctggcc accaccggca tttatttatt gatcaacttg gtttacgtcc
      961 tgtatttgcc cttggacgag atgcgcgtga cggatttggt ggcggcgggg gtcatggata
     1021 agatatggcc ccgggggggc gggtcgatcg tggccgccat ggtcatggtg tccaccttcg
     1081 gggcgttgaa cggatttatt ttttccagcg gccgtttgct ctcggccctg ggccaagacc
     1141 acgccctgtt caaaaaaatg ggcgtcgtgt ccgcccgcac ccaaaccccg gcggccgccc
     1201 tggcggccaa cgccgtcctc gccctggttc tggtgtggac cgggaccctg gacaaaatcg
     1261 tgacctacac ggaggtcgtg atttatttat tttttgcggc gacgggggtt tcgctgttcg
     1321 ttttccgacg acgggaaggg gcggccccgg gcgggtaccg cgtgtggggc tatccgtgga
     1381 cgcccctggc gtttattctg ctcaacgtcg ccatcgccct gaacggcgtg gccgaggaac
     1441 cggcggtggc cctggcgggc atcggcgtgg cggccctcgg gttccccctc tattgggcgt
     1501 cccggcgcct cggaccgaaa acctaatcgc gcatcttttt tacaaaccgc ttcaaccccc
     1561 gaaacatcgc ctccgcgcag tcgcactgaa acgtttccgt tttaaggagt tcctcctcgg
     1621 gcggccaaat cacgtacgcc gattccgtca acaccgccgg catttgggag gtgcgggcca
     1681 gggccaggtt gccgtagtgc agtccgtcgt cccgcaaatg ggtggagcgg aaggcctgag
     1741 cccgaccgaa aatgtccccg tacgcctcgt ggatttccgt cgccagatct tcgctttggg
     1801 ggtggtaata gtagaccccg taaccgttcc gctcgaaggg attggcgccg tcgggaaggg
     1861 cgttgttgtg cacgctgatc aaaacgtccc cccgggcggc ccaggccagt ttcggccgat
     1921 cgtataaatt gacgttttcc gacccgtatc gaagcatcac cacgtcggcc ttttccgatc
     1981 gcaattttcg ttccagacat ttggcgatgg ccaaattggc gtccttttcc agtaggcccg
     2041 tcggtccccg ggaaccgatg tcccaggaat gccccgcgtc caccacgatc ttaagcccct
     2101 ccagggatcc gtctttgggg cctgggggcg ga
//
LOCUS       DAOQLS010000362         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_34485_length_2127_cov_3.251931, whole genome shotgun sequence.
ACCESSION   DAOQLS010000362 DAOQLS010000000
VERSION     DAOQLS010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34485_length_2127_cov_3.251931"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..401)
                     /locus_tag="PK362_07165"
     CDS             complement(15..401)
                     /locus_tag="PK362_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNC74644.1"
                     /translation="MKRTTRWGSTAFLAMALAVVNGAAANKKTMPSPAKTSTESPADL
                     LAAGVYTAKMKAFVCGGCADWVKERLTSVPGLSNVRAEQSTARVEFTVDKPVSRKLIQ
                     KTLDGAAREMGMGADYTLDGLKPKTK"
     gene            complement(411..>2127)
                     /locus_tag="PK362_07170"
     CDS             complement(411..>2127)
                     /locus_tag="PK362_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC74645.1"
                     /translation="RWFSKIWNAATVGRYYAEEKHPVSRVLFKIYDPACRWVLRYPRR
                     TIAGALLLVAATVPVYFRLGTEFMPPLNEEVLLYMPTTMPGLSVTEAQRLLQTQDKIL
                     KGFPEVERVFGKAGRADTATDPAPFSMMETTVVLKPRDQWRPKERWYSFLPGVLKAPF
                     RRFWPDRMSQEELVRDMDEALRLPGTVNAWTMPIKARIDMLTTGVRTPVGVKVFGADL
                     KVIEKIGRRLEEILSPVPGTRSVYAERTAGGYFLDVALRRDRLARYGLTVQEAQMVLM
                     SAVGGEPVTTTVEGRERYSVNVRYAREYRDDLPKLRRVLVPTMSGAQVPLGQLADISL
                     VDGPSMIRDENGLLSGYVYVDLAGRDVGGYVEEAKKVVREKLDLPAGYALVWSGQYEN
                     MLRVRERLKIVVPVTLFLIFLLLYMNTRSTAKAGLVMLAVPFSMIGAVWYLWLLGYNV
                     SIAVWVGIIALLGLDAETGVFMLLFLDLSHDEALRAGRLRNAGELDEAIVHGAVKRVR
                     PKMMTVAAAMMGLLPLMWSTGTGADMMKRVAAPMIGGLVTSFVLELLVYPAVYKMWKG
                     KNLKA"
BASE COUNT          402 a          686 c          643 g          396 t
ORIGIN      
        1 atgcggggat tagatcattt cgttttgggt ttcagtccgt ccaacgtgta atcggcgccc
       61 atgcccattt cgcgggcggc gccgtccagg gttttttgga tcaatttccg ggagaccggt
      121 ttgtccacgg tgaattccac ccgggccgtg gactgttccg ccctgacgtt ggaaagaccg
      181 ggcaccgagg tcaatcgttc cttgacccaa tccgcgcacc ccccgcagac aaaggccttc
      241 attttggccg tgtaaacgcc cgccgccaga aggtcggctg ggctttccgt cgaagttttt
      301 gccggggagg gcatcgtttt tttgttcgcc gccgccccgt tgacgaccgc caacgccatc
      361 gccaaaaacg ccgtcgaccc ccatcgtgtt gtccgtttca taagtctcct ttacgcctta
      421 agatttttgc ccttccacat tttataaacg gccggataga cgagcaattc cagaacaaaa
      481 ctggtcacca ggcccccgat catgggcgcg gccacgcgct tcatcatgtc cgccccggtg
      541 ccggtggacc acatgagggg caacaggccc atcatggccg cggccacggt catcattttg
      601 gggcgcacgc gcttgaccgc gccgtggacg atggcttcgt ccagttcccc cgcgttccgc
      661 aggcggccgg cccgaagggc ctcgtcgtgg gagaggtcca ggaaaagcag catgaagacc
      721 cccgtctcgg cgtccaggcc caggagggcg atgattccca cccacacggc gatggacacg
      781 ttgtagccca gaagccacag ataccacacg gcgccgatca tggaaaacgg cacggccaac
      841 atcacgaggc ccgctttggc ggtcgagcgc gtgttcatgt agagaagcag gaagatgaga
      901 aagagcgtga cgggcacgac gattttgagc cgctcccgca cccggagcat gttctcgtac
      961 tggccgctcc acaccagggc gtacccggcc gggaggtcca atttttcacg caccactttt
     1021 ttggcctcct ccacgtagcc gcccacgtcc cggcccgcca gatcgacgta cacgtaaccc
     1081 gacaacaggc cgttctcatc ccgaatcatg gaagggccgt ccaccaagga aatgtccgcc
     1141 agctggccca ggggcacctg ggcgccggac atggtgggga ccagcacccg ccggagtttg
     1201 ggcaggtcgt cccggtattc ccgggcgtag cgcacgttga ccgaataacg ctcgcgcccc
     1261 tccaccgtcg tggtgacggg ctcgccgccc accgccgaca tcaggaccat ttgggcctcc
     1321 tgaacggtca atccgtaacg ggccaggcgg tcccgccgca gggcgacgtc cagaaaatag
     1381 ccgcccgcgg tccgttccgc atacacgctc cgagtgccgg ggacggggga cagaatttcc
     1441 tccaaccgcc ggccgatctt ttcgatgacc tttaaatcgg cgccgaagac tttcaccccg
     1501 acgggcgttc gaacgccggt ggtgagcatg tcgatccggg ctttgatggg catggtccag
     1561 gcgttgacgg tcccgggcag gcgcagggct tcgtccatgt cccggaccaa ttcctcctgg
     1621 gacatccggt ccggccagaa ccgccggaag ggggccttca ggacccccgg taggaaggaa
     1681 taccaacgtt ccttgggtcg ccattggtcc cggggtttca ggaccacggt ggtttccatc
     1741 atggagaaag gagcggggtc cgtggcggta tcggcccggc cggccttgcc gaacacccgc
     1801 tccacctccg gaaacccctt cagtattttg tcctgggttt gaagcaggcg ctgggcttcg
     1861 gtgacggaga gtcccggcat ggtggtgggc atgtagagaa gcacctcttc gtttaagggc
     1921 ggcatgaact ccgtgcccag gcgaaaatag acgggcacgg tggccgccac cagaaggagc
     1981 gcccccgcaa tggttcgccg gggataacgc aacacccaac ggcaggccgg atcgtagatt
     2041 ttaaagagga cccggctcac ggggtgtttt tcctctgcgt aataacgtcc cacggtcgcg
     2101 gcattccaga ttttggaaaa ccaccgg
//
LOCUS       DAOQLS010000363         2126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_34510_length_2126_cov_5.314824, whole genome shotgun sequence.
ACCESSION   DAOQLS010000363 DAOQLS010000000
VERSION     DAOQLS010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34510_length_2126_cov_5.314824"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1284
                     /locus_tag="PK362_07175"
     CDS             58..1284
                     /locus_tag="PK362_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC74646.1"
                     /translation="MAQFAYKARATSGSVLSGMLEANDQRAAIDQLRGQRLIVLEINE
                     SAPGFLDAIKKSALFKPKVAQKDIVLFSRQLSTLVSAGVALVSGLNILQEQIINKTFR
                     DIVLKVKEDIESGLPIADALKKHPDAFTDLYVAMIKAGEVGGILDVILERLSNYLEAA
                     EQLRGKVKGAMMYPAVVSTIAAAVTVFLLVGVIPTFKEIFSSFGAELPLPTRIVIGIS
                     EALQQQWYVFVGVPIGGFILFKRWVKTEKGAQIFDTQVLKMPLFGPMLRKVAVAKFTR
                     TLGTLVKSGVPILQAMETVAQTAGNKVIEKAVMSARESIREGERIAEPLKKSGVFPPM
                     VVQMIAVGEETGNMDIMLNKIADFYDQEVDVAVKGLTSMIEPIVIVFMGIVIGGIVIA
                     MFIPMFELGQLAGKQG"
     gene            1439..1858
                     /locus_tag="PK362_07180"
     CDS             1439..1858
                     /locus_tag="PK362_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC74647.1"
                     /translation="MKKVAKGFTLIELMIVVAIIGILAAIAIPKFADLVTKSKEASIK
                     GSLGTIRSAVSIYYSDQEGVYPASGSLAIALTSGSRYLQEMPYVQIPQGAYGHSRVNT
                     ETTVNADGGNWLYAPGSGQVAVNCTHTDTKSSTWSTW"
     gene            2004..>2126
                     /locus_tag="PK362_07185"
     CDS             2004..>2126
                     /locus_tag="PK362_07185"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNC74648.1"
                     /translation="MLLSGMAGAALGSFANVCIHRIPRGESVVFPGSHCPGCRTP"
BASE COUNT          469 a          596 c          598 g          463 t
ORIGIN      
        1 ccttcgcccc tggacagaac gcccaaagtc ggttacattg atgaatagga aatagacatg
       61 gcccaattcg cctacaaagc ccgtgccacc tccggaagcg tcctctccgg aatgttggaa
      121 gccaacgatc aacgcgccgc gatcgatcaa ttgcgtggcc agcgtctcat cgttcttgaa
      181 atcaacgaaa gcgccccggg ttttctcgac gccatcaaaa agtcggccct ctttaaaccc
      241 aaggtggccc aaaaagacat cgttcttttt tcccgtcaac tttcaacgct ggtgtccgcc
      301 ggcgtggcgt tggtgtccgg gttgaacatt ctccaggaac aaatcatcaa caaaactttt
      361 cgcgacatcg tcttgaaggt gaaggaagac attgaatccg gtctcccgat cgccgacgcg
      421 ttgaaaaagc acccggacgc cttcacggat ctttacgtgg cgatgatcaa ggcgggcgaa
      481 gtcggcggta ttttggacgt cattttggag cggttgtcca actacctcga agccgccgag
      541 cagcttcggg gaaaagtcaa aggggccatg atgtaccccg ccgtcgtcag tacgatcgcc
      601 gccgccgtaa cggtcttcct tctggtcggc gttattccga cctttaaaga aatcttttcc
      661 agcttcgggg cggaattgcc gcttccgacc cgaatcgtga tcggcatttc cgaagccctt
      721 cagcagcagt ggtatgtttt tgtcggggtt ccgatcgggg ggtttatcct gtttaagcgg
      781 tgggtcaaaa cggaaaaagg cgcccagatt ttcgacaccc aggtgctcaa aatgcccctt
      841 tttgggccca tgttgcggaa ggtcgccgtg gccaaattta cccggacgtt ggggaccttg
      901 gtcaagtccg gcgtgcccat tctgcaggcc atggaaacgg tggcccaaac ggccggcaac
      961 aaggtcattg aaaaggccgt catgagcgcc cgggaatcca tccgggaggg cgaacgaatc
     1021 gccgagccct taaagaaatc cggggtgttc ccgcccatgg tggttcaaat gatcgcggtg
     1081 ggggaagaaa ccgggaacat ggacatcatg ctcaataaaa tcgccgattt ttacgaccaa
     1141 gaagtggacg tggccgtcaa aggcctcacc tccatgatcg aacccatcgt catcgttttc
     1201 atgggcatcg tgatcggcgg catcgtcatc gcgatgttca tcccgatgtt cgagctcggc
     1261 caactggccg gcaagcaggg ttaaaagtaa aggacttgaa aatccccggt tgggggggta
     1321 agcttacatg aaggtcttcg cttgacagtg tttcgggcga atgatatttt caccgcaact
     1381 ttacgatttc tcggcggcaa agatcgtcga gaaggaggtg agcaacatga gtcaacttat
     1441 gaaaaaggtc gcgaaagggt tcaccctgat cgaattgatg atcgtggtgg caatcattgg
     1501 tatcttggcg gctatcgcca tccctaaatt cgccgacctc gtgacgaagt cgaaagaagc
     1561 cagcatcaaa ggcagcttgg gaaccatccg cagcgccgtg agtatttatt actccgacca
     1621 agaaggcgtg tatcccgcct cgggttcctt ggcgattgcc ttgaccagtg gctcgcgcta
     1681 cctgcaggaa atgccctacg tgcagattcc ccagggcgcc tacgggcaca gccgcgtcaa
     1741 cactgagacc accgttaacg ctgacggcgg gaactggctg tatgccccgg gttccggtca
     1801 agtggcggtc aactgcaccc acaccgacac caagagctcg acctggagca cctggtaagt
     1861 ccgagcgtcc aacgtttcaa aagcggggga gggatcttcg gatccctctc ccgcttgttt
     1921 tttaaggggg gccttggccc gccgcatcgg gaaggagcgc gtttgtggat tcagacaccg
     1981 gggcgttgac gcccctcgtt cgcatgttat tgtccggaat ggccggcgcc gccttgggca
     2041 gtttcgccaa cgtgtgcatc catcgaattc cacgggggga atccgtcgtt ttccccggct
     2101 cccattgccc tggctgccgg acccca
//
LOCUS       DAOQLS010000364         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_34810_length_2116_cov_4.318292, whole genome shotgun sequence.
ACCESSION   DAOQLS010000364 DAOQLS010000000
VERSION     DAOQLS010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34810_length_2116_cov_4.318292"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PK362_07190"
     CDS             <1..472
                     /locus_tag="PK362_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC74649.1"
                     /translation="TIPMAMIGSIPLLWITHTSATMSVYIGMIMLGGIAVSAAIILVE
                     KINQSRAEGTPLMKAVLKSSWLRLSPILNTSLTTIVDLIPMALSRSESASLWAPLSLT
                     VIGGATMSTFLTLFMIPALYYHIEAFKERFMGRVAAAEVPAIAGETSASALQPQ"
     gene            complement(477..839)
                     /locus_tag="PK362_07195"
     CDS             complement(477..839)
                     /locus_tag="PK362_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74650.1"
                     /translation="MTRRQVWLLSAVALLTALLAGGVFFASLNEEKKRRSLNGPGPFP
                     GSLEDRERTLREIEALRKLNKEGKIPPPGVDPRIQEALQTVQEINRINQVNQQNRQRL
                     EKPILPPPPTAKPVKKSE"
     gene            complement(867..1103)
                     /locus_tag="PK362_07200"
     CDS             complement(867..1103)
                     /locus_tag="PK362_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74651.1"
                     /translation="MDNKPLKYIPLAFKALALISAALGLVFFVIILIGGGTPDAPRVT
                     SVLALILGFFYLLFFWTIAEVIGLLIKIESNTRR"
     gene            1480..>2116
                     /locus_tag="PK362_07205"
     CDS             1480..>2116
                     /locus_tag="PK362_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74652.1"
                     /translation="MQDQLSQFRRALFAPIPHTPPVEKFLPRLETLRRALALALAIVM
                     FHSQVLCAGTTSLPLWPSAQGVVARPGFLTRLIARLGGKAKQTPYLFRDSMSSDRRRL
                     LHPEEESGVSPDTLKRLEGAQNGRADIAGQNQLNTGFRDLENARRPDYATGAGALGQI
                     NELEKALTPADLAAKSIQEVIEAIRTKMKNLFGQGAEFKFDLNKNFDGTYSR"
BASE COUNT          478 a          616 c          568 g          454 t
ORIGIN      
        1 caccattccc atggcgatga ttggaagcat tccgctgctt tggatcaccc acacgtcggc
       61 gaccatgagc gtttatatcg gaatgattat gctggggggg attgccgtca gcgcggcgat
      121 tattttggtc gaaaaaatca atcaatctcg ggcggagggg accccgttga tgaaagcggt
      181 gttgaaatcc agctggctgc gcctctcccc gatcctcaac acgtccctca ccaccatcgt
      241 ggaccttatt cccatggccc tgagccgaag cgaatccgct tccctttggg cccccttgtc
      301 cttgacggtc atcgggggcg ccaccatgtc cacgttcctg actctcttca tgattccggc
      361 tctttattac cacattgagg cgtttaagga gcgctttatg gggcgtgtgg cggccgcgga
      421 agtccccgcg atcgcggggg agacgtccgc gtcggctttg cagccgcaat agggtattat
      481 tcggattttt tgacgggttt cgccgtcggc ggggggggga ggatgggctt ctccagccgc
      541 tgacgattct gctgattgac ttgattgatg cggttgattt cctgaacggt ctgcaacgcc
      601 tcttgaatcc gcggatccac cccgggcggg gggatctttc cctccttgtt tagcttccga
      661 agggcttcaa tttcacgaag ggtgcgctcg cgatcttcca gcgatccggg gaagggtccc
      721 ggtccgttga gggaacggcg tttcttttcc tcattgagac tggcaaaaaa aaccccgccg
      781 gccaggaggg ctgtcagaag cgccacagcg ctcaacaacc acacctgccg gcgggtcatg
      841 aaatcaccga ttcccgaagg aaccgtctaa cgacgggtgt tggattcgat cttgatcaac
      901 aggccaatga cctccgcgat cgtccagaag aaaaggaggt aaaagaagcc caagatcaag
      961 gcaagaaccg acgtgacccg aggggcgtcc ggcgtaccgc caccaatgag aatgatgacg
     1021 aagaaaacca atcccaacgc cgcggaaatc agcgccaggg ccttaaacgc caggggaata
     1081 tacttcaggg gcttgttgtc cattcggtcg aaacctcctc gagaataaaa acgcaggatg
     1141 aatacgatac gccggtttcc cgacgtggcc tgttaaatct ataacagact cccaaccctg
     1201 tcaaggggaa atttaaacgg gccaaaatcg acaatttaat aaacacaatt taacgcgttt
     1261 ggttgatgtg ttgcatgtgt cattgggtat gaagttcgac caaaacaacg gcacgggcgc
     1321 tcaaaaccgc ataaaactcc ctgttacaat caaaacatca ttcagtttca tttcatttac
     1381 ctgcattttc cgattagttt acatctactt aaggccttct taaggcccgg gcactatatt
     1441 agaagggtca tgaagccatt ttccttaggg ggaaaatcca tgcaagacca actctcgcag
     1501 tttcgccgcg ctttgttcgc gcccattccg cacacgccgc ccgtcgaaaa attccttccg
     1561 cgcctggaaa cgctacgacg ggcgttggcg ttggcgttgg ccatcgtcat gttccattcc
     1621 caggtgctct gcgccggcac gaccagcctc cccctctggc cgtccgccca aggcgtcgtc
     1681 gcacgtcccg gcttcctgac ccgtttgatc gcccgcctcg gcggcaaagc gaaacagacc
     1741 ccctatctgt tccgcgacag catgtcgagc gatcggcgtc gccttttgca ccccgaagag
     1801 gagtccggcg tctccccgga caccctcaaa cgcttggaag gcgcgcaaaa cggccgggcg
     1861 gacatcgccg gccaaaatca gttgaacacc ggttttcggg acctggaaaa tgcccggcgg
     1921 ccggactacg ccacgggcgc cggcgctttg gggcagatca acgaactgga aaaagccctg
     1981 acccccgcgg atttggccgc caaatcgatc caagaagtga tcgaggccat tcggaccaaa
     2041 atgaaaaacc tctttggtca aggcgcggag ttcaagttcg acctgaataa gaactttgac
     2101 ggcacctatt cacgga
//
LOCUS       DAOQLS010000365         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_34865_length_2114_cov_11.233609, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000365 DAOQLS010000000
VERSION     DAOQLS010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34865_length_2114_cov_11.233609"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1027)
                     /locus_tag="PK362_07210"
     CDS             complement(74..1027)
                     /locus_tag="PK362_07210"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HNC74653.1"
                     /translation="MAMYPIEAQRFGIKKEAVRGTAETTPTSWFPVLKGTEFNYSLGL
                     LDNDVLKGDPTMLAPVAGKKSGAGKIKLPLDAQTCGEVFYSLLGGKAAAQQGGTAAYK
                     HTFTVAAGLQKPSYTFFYDYGLDIKKYALGIVKKVSLSGAVDSVGALEADILFKNEAT
                     GSIGSPTFPTQRYLAFNQMDFKVAGTSNTDIKDWNLEIDNGAAPLWTLNQSQDCIDVV
                     TGGKMDIKGGFTIYFQNETERAKFLANTAVALRMIATGPLIASTYYYTVDLNVYAAHY
                     TAFPFGDDSGLLAAKVAFTGYYSASDSKAIQVDVTNITTAY"
     gene            complement(1030..1209)
                     /locus_tag="PK362_07215"
     CDS             complement(1030..1209)
                     /locus_tag="PK362_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74654.1"
                     /translation="MMDNYKTKDMKDKPSKLKCVAECWAPGLGFFKVGEILSGAELVE
                     KLNGNPNFIEITEEK"
     gene            complement(1206..1658)
                     /locus_tag="PK362_07220"
     CDS             complement(1206..1658)
                     /locus_tag="PK362_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74655.1"
                     /translation="MASKRRTILEYLRDTRLPLITVAGGYHTNVQHIQRGLYSPNGLN
                     DSDLPALLIGRTVEKRENITRNQYKAIITVYILGIVKSPDGVSNAQGALDDMIADVTH
                     ALETDRTLGGNSKWLEVKNITTDDGDIAPRAGFLMEVEIAYVTEGVTP"
     gene            complement(1648..>2114)
                     /locus_tag="PK362_07225"
     CDS             complement(1648..>2114)
                     /locus_tag="PK362_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74656.1"
                     /translation="ALVSRLIKRACGDYLERARTTAINEHLTKRGPETLGVYRGSLST
                     RLAGEVSESGNFIFASLGSPMPYARAWEYGRPPGQQPPSAPLAEWAKKIMGVSDKEAK
                     SVGFLIARSIGRKGRPARPFLRPALEKNIQYLTDDITTLIQGAAEGGWKRGV"
BASE COUNT          445 a          636 c          527 g          506 t
ORIGIN      
        1 tcccccaccc gcgcccgcgc ctcaagcgcg agccgggcat ctggggacaa cctcattatt
       61 tccttagcgg tcattagtag gccgtggtga tgttcgtcac gtcgacctgg atcgctttcg
      121 aatccgacgc ggagtaatag ccagtaaagg ccaccttggc cgccaacaac cccgaatcgt
      181 cgccgaacgg gaaggccgtg taatgagccg cgtaaacgtt caaatccacc gtgtaatagt
      241 aggtggaagc gataagcggc cccgtcgcaa tcatccgaag ggcgacggcg gtgttggcaa
      301 gaaacttcgc ccgctccgtt tcgttttgga agtagatcgt aaacccgccc ttgatgtcca
      361 tcttgccgcc cgttacgacg tcgatacagt cctgggactg gttcaacgtc cacaaaggag
      421 ccgcgccgtt gtcgatttca aggttccagt ccttgatatc ggtgttcgat gtgccggcca
      481 ccttaaagtc catctggtta aacgccaaat atcgttgggt cgggaaggtt ggggagccaa
      541 tagaaccagt tgcctcattc ttgaacagaa tgtccgcttc caacgcccca acgctatcca
      601 ccgcaccgga caaggacacc tttttgacga tcccgagggc atacttttta atgtccagcc
      661 cgtagtcata aaagaacgtg tatgaaggct tttggagccc ggccgcgacg gtgaacgtgt
      721 gcttgtaagc cgccgtaccg ccctgctgtg cggccgcctt tccaccgaga agagagtaaa
      781 acacctcccc gcaagtctgg gcgtcaagcg gaagtttgat tttgcccgca ccgcttttct
      841 taccggccac aggggccaac atcgttgggt cgcccttcaa aacgtcgttg tcaaggagcc
      901 cgagcgaata gttaaactcc gtccctttta atacaggaaa ccacgacgtc ggcgtcgttt
      961 ccgccgtgcc tctaaccgcc tccttcttaa tcccaaaccg ttgagcttcg atcggataca
     1021 tcgccatttt tatttctcct ctgttatttc gataaagtta ggatttccgt tcaatttctc
     1081 aaccaattcc gcaccgctta gaatctctcc caccttgaaa aatccaagac cgggagccca
     1141 acactccgcc acgcatttca gctttgacgg tttgtctttc atgtcttttg ttttgtaatt
     1201 gtccatcaag gcgtcacccc ttcggtcacg taggctatct caacttccat aagaaatccg
     1261 gcacgtggcg caatgtcccc gtcgtccgtc gttatatttt taacttcgag ccatttggaa
     1321 ttaccgccca atgttctgtc cgtctcaagg gcgtgcgtca cgtcggcaat catgtcgtca
     1381 agggccccct gggcgttgct caccccgtcc ggagatttaa cgatcccgag aatgtagacc
     1441 gttatgatgg ccttgtattg gttgcgggtg atgttttccc gcttctcaac cgtccgtccg
     1501 atcaacaacg ccgggaggtc tgaatcattc agcccattgg gtgagtaaag ccccctctga
     1561 atgtgttgga cgttcgtgtg atacccgccc gccaccgtaa tcagtggcaa ccgagtgtca
     1621 cgtaaatatt caaggattgt ccgccgctta gacgccacgt ttccacccgc cttccgccgc
     1681 gccctggatc agcgtcgtaa tatcgtccgt caaatactgg atgttctttt cgagtgccgg
     1741 gcggaggaac ggacgggccg gccggccttt cctcccgatg ctccttgcaa ttaaaaagcc
     1801 gacgctcttg gcctctttgt ccgaaacgcc catgatcttc ttggcccact ccgccaaagg
     1861 cgcgctgggc ggctgttgac cgggaggtcg accgtattcc cacgcgcgcg cgtatggcat
     1921 gggggaacca agcgacgcaa atatgaaatt tccggattca gatacttccc cagcaagtcg
     1981 cgtcgaaaga gaccctcggt agacaccaag agtttccggc ccccgcttcg tcaagtgttc
     2041 gtttatggcc gttgtccgtg cccgctcaag ataatcacca caggcccgct ttatcagacg
     2101 cgaaacaagc gcgg
//
LOCUS       DAOQLS010000366         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_34930_length_2112_cov_4.997569, whole genome shotgun sequence.
ACCESSION   DAOQLS010000366 DAOQLS010000000
VERSION     DAOQLS010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34930_length_2112_cov_4.997569"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2112)
                     /gene="acnA"
                     /locus_tag="PK362_07230"
     CDS             complement(<1..>2112)
                     /gene="acnA"
                     /locus_tag="PK362_07230"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNC74657.1"
                     /translation="HVAMTRKIGGEDWVYPDTLVGTDSHTTMINGLGVLGWGVGGIEA
                     EAAMLGQPMSMLLPEVVGVRLTGRLPAGATATDAVLTVTQILRKKNVVGKFVEFVGPG
                     LDQLSLPDRATIANMAPEYGATMGFFPVDTETLAYLRLTGRSEDDVRRTESYLKAQGM
                     FRSPTSPDPEYSDLLELDLSTVVPSLAGPKRPQDRVPLSDAKASFQKSLAAPVKDRGF
                     EIPAEEVSRKVRVESNGHVTTIGHGAVVLAAITSCTNTSNPSVLIAAGLLAKKAVERG
                     LAVAPHIKTSLAPGSRVVTDYLNAAGLSTYLDKLGFQLVGYGCTTCIGNSGPLPEAVS
                     KAVKEGNLVVSAVLSGNRNFEGRINPHVKANYLASPPLVVAYALAGRLDLDLTRDPLG
                     IGSDGSSVFLKDLWPSETEIRDVLQKAVRADMFRERYRTVADGDDHWNSLSVPTGDLY
                     AWDPASTYIKHPPYFDGMTLESGVLADLKGLRPLAVLGDSVTTDHISPAGSIAEDSPA
                     GKYLIGLGVTKKEFNSYGARRGNHEVMIRGTFANIRLKNLMVPGVEGGVTLHRPSGER
                     MPIYDAAQRYAAENVPLLVIAGKEYGTGSSRDWAAKGTRLLGVRAVIAESYERIHRSN
                     LVGMGVLPLQFLPGETRDTLGLTGRETFDIAGVAGLTPKAKLTVTVRREDGSAATFQA
                     VARVDTPVEVEYLAHGGILPAV"
BASE COUNT          353 a          666 c          722 g          371 t
ORIGIN      
        1 caccgcgggc aagatgccgc cgtgggccaa atactcgact tccaccggcg tgtccacccg
       61 ggccacggcc tggaaggtcg cggcggagcc gtcctcccgt cggaccgtca ccgtgagttt
      121 cgctttgggc gtcaatcccg cgacgcccgc gatgtcgaag gtttcccgcc ccgtcaaccc
      181 gagcgtgtcc cgggtttccc cgggcaaaaa ctgaaggggc agcacgccca tgcccaccag
      241 gttggaccgg tggatccgct cgtagctttc cgcgatgacc gcccgcacgc ccaggagccg
      301 cgtgcccttg gccgcccagt cccgggagga cccggtgccg tattccttcc cggcgatcac
      361 caggagcgga acgttttcgg ccgcgtagcg ctgggccgcg tcgtaaatgg gcatccgttc
      421 gcccgacggc cggtggaggg tgacgccgcc ttcgacgccc ggcaccatca ggttcttcag
      481 tcggatgttg gcgaacgtcc cccggatcat gacctcgtgg ttgccgcgac gggcgccgta
      541 ggaattaaat tcctttttgg tcacgcccag gccgatgagg tatttgcccg cggggctgtc
      601 ctcggcgatg gacccggccg gggaaatgtg gtcggtcgtc acggagtcgc ccaacaccgc
      661 caacggacgc agtcctttca ggtcggccag gacgccggac tccagggtca tgccgtcaaa
      721 gtagggcggg tgtttgatgt aggtggaggc cgggtcccag gcgtaaaggt cgccggtggg
      781 aacgctcagg gaattccaat ggtcgtcgcc gtccgccacg gtgcggtaac gttcccgaaa
      841 catgtcggcc cggacggcct tttgaagcac gtcccggatt tccgtctcgg acggccaaag
      901 gtccttgagg aaaacggaac ttccgtcgga cccgatgccc agggggtccc gggtcaaatc
      961 cagatccagg cggcccgcca gggcgtaggc gaccaccaaa ggcggggagg ccaggtagtt
     1021 ggctttgacg tgggggttga tgcgcccttc gaagtttcgg ttgccggaga gcaccgcgct
     1081 gacgacgagg ttgccctctt tgacggcctt ggaaacggcc tcgggcaggg gcccgctgtt
     1141 gccgatgcac gtcgtgcagc cgtagcccac cagttgaaaa cccaacttgt ccagataggt
     1201 cgaaagcccc gcggcgttca aataatccgt caccacccgg gaccccgggg ccaagctggt
     1261 tttgatgtgg ggagccaccg cgaggccgcg ctcgacggcc ttcttggcca ggagcccggc
     1321 ggcgatcaag accgaggggt tggaggtgtt cgtgcagctg gtgatggcgg ccaggacgac
     1381 ggcgccgtgg ccgatggtcg tcacgtgccc gttggattcc acccggactt tgcgggagac
     1441 ttcttcggcc gggatttcaa aaccgcggtc cttgacgggg gccgccaggg atttttggaa
     1501 ggacgccttc gcgtcggaca ggggcacccg gtcctggggc cgcttcgggc cggccaggga
     1561 ggggacaacg gtggaaaggt ccaattccaa caaatcggaa tattccgggt ccggcgacgt
     1621 cggggaacgg aacatcccct gggccttgag gtagctttcc gtccgccgaa catcgtcttc
     1681 gcttcgcccg gtgaggcgaa ggtaggccag ggtttccgtg tccacgggga aaaagcccat
     1741 ggtggcgccg tattcgggcg ccatgttggc gatggtggcg cgatcgggca gggagagctg
     1801 atccaacccc gggcccacga attcgacgaa tttaccgacg acgttctttt ttcggagaat
     1861 ctgggtcacg gtcaaaaccg cgtcggtggc cgtggccccg gcgggaaggc ggccggtcag
     1921 gcgcacgccc acgacttccg gcaggagcat ggacatcggt tggccgagca tggcggcctc
     1981 ggcctcgatg ccgcccacgc cccagcccag aacgcccagg ccgttgatca tcgtggtgtg
     2041 tgagtcggtg ccaaccagcg tatcggggta aacccaatct tcgcccccga tcttgcgggt
     2101 catcgcgacg tg
//
LOCUS       DAOQLS010000367         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35047_length_2109_cov_4.415287, whole genome shotgun sequence.
ACCESSION   DAOQLS010000367 DAOQLS010000000
VERSION     DAOQLS010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35047_length_2109_cov_4.415287"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK362_07235"
     CDS             <1..268
                     /locus_tag="PK362_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC74658.1"
                     /translation="DGWNGVAGPVKGLFYGDASQFVAQLIGVGANLAYVGVMGYIVFK
                     LIDVTVGHRVNPMDEVEGLDIPEMGLPGYMGVKMDKYSETPMSR"
     gene            282..623
                     /locus_tag="PK362_07240"
     CDS             282..623
                     /locus_tag="PK362_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNC74659.1"
                     /translation="MKKIEAIIRREKLDAVKKALDAIKTPGITMYEVLGHGKQKGITE
                     QFRGREFRVDFLPKTKIEVVVEDSHLRSTLDAILKAAATGQVGDGKIFVSTIDDVIRV
                     RTGESGALAVA"
     gene            1013..>2109
                     /locus_tag="PK362_07245"
     CDS             1013..>2109
                     /locus_tag="PK362_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC74660.1"
                     /translation="MVDRACSDKIAAAKPAVDTGDTAWILISAALVLMMTPALAFFYG
                     GLVRRKNMLSILMQCYMLMGLITVEWVLVGYTLSFGPDHHGLIGGLDWFGLCGVGLAP
                     NADYAATIPHQAFMVYQCMFAIITPGLILGAFAERMKFSAFCVFSLLWALVVYNPVAH
                     WVWGVGGFLRQMGALDFAGGAVVHVNAGMAALASALYLGKRKGFPTHISPPHNLPFAV
                     LGAGLLWFGWFGFNGGSALGSGALATNAFVTSHIAAGTAGLVWALLDWKVNSHPTMLG
                     TITGAVAGLVAITPAAGFVDPLGALAIGVTVAIICFVSVMYVKGKFGYDDSLDAFGVH
                     GVGGFWGALATGLWATKAVNAAGADGLFHGNP"
BASE COUNT          353 a          676 c          678 g          402 t
ORIGIN      
        1 cgacgggtgg aacggcgtgg cgggccccgt gaagggcctg ttctacggcg acgcctccca
       61 gttcgtggcg caattgatcg gcgtgggcgc gaacctggcg tatgtcggcg taatgggcta
      121 cattgtcttc aagctcatcg acgtgaccgt cggccatcgg gtgaacccca tggacgaagt
      181 ggaaggtttg gatattccgg aaatgggact gccgggctat atgggcgtta aaatggacaa
      241 atactccgaa acgcccatgt cccggtaagg ggaggaacct tatgaaaaaa atcgaagcca
      301 tcatccggcg cgagaaactc gacgccgtta aaaaggcgct ggacgccatc aaaaccccgg
      361 gcatcacgat gtatgaagtc ctggggcacg ggaagcaaaa gggcatcacg gaacagttcc
      421 gcggacggga atttcgcgtg gacttcctgc ccaaaaccaa gatcgaagtg gtggtcgagg
      481 acagccacct ccggtccacg ttggacgcca ttctgaaggc ggccgcgacc ggccaggtgg
      541 gcgacggaaa aatcttcgtc tccaccattg acgacgtgat ccgggtgcgc accggcgaaa
      601 gcggggccct ggccgtcgcc taacgcgaca agttcaaccg ataagcgagg ggagggggcc
      661 atgcgccccc tcccctttgc tttttcccgc cggttatctt agaatctccg cgacacaacg
      721 gcgtgtcgcc cgcggacccc cccaacgttg ccggggttcg cgcccgtcat ggggatgata
      781 ggggacgcgc cgggacagag ttgtcccgct ttcgaaccga tcgaaagcgg gatttttatt
      841 gcctgccatc gcctttcttg cccccgggac atttcacgaa ggaggtttca gcgtcatgcg
      901 gaaacccttg ctttggatca ccttgctttt cgccgcggcc gcgccgttgt tggcccacgc
      961 cgcgtcgaat ttgaccgggc tttcggaaaa ccaaacccgc gaagtggcgg cgatggtcga
     1021 ccgggcctgt tcggataaaa tcgccgcggc caaacccgcc gtggacaccg gcgacaccgc
     1081 gtggatattg atctccgcgg cgttggtctt gatgatgacc cccgccctgg cgttcttcta
     1141 cggcggcctg gtccggcgaa aaaacatgct ctcgatcctc atgcagtgct acatgctcat
     1201 ggggctgatc accgtggagt gggtgctggt cggctacacg ttgtccttcg gccccgacca
     1261 ccacgggctg atcggcggcc tggattggtt cgggctgtgc ggcgtggggt tggcccccaa
     1321 cgcggactac gcggccacca ttccccacca ggccttcatg gtctaccagt gcatgttcgc
     1381 catcatcacc ccgggcctca tcctgggcgc cttcgcggaa cggatgaagt tttccgcctt
     1441 ctgcgttttc tcgctcctgt gggccctggt cgtctacaac cccgtggccc actgggtctg
     1501 gggcgtgggc ggatttctcc gccaaatggg cgccctggat ttcgcgggcg gcgcggtggt
     1561 ccacgtgaac gccggcatgg cggccctggc ctccgccctc tacttgggaa agcggaaagg
     1621 ctttccgacg cacatttccc ccccgcacaa cctgcccttc gcggtgttgg gggcgggcct
     1681 gctgtggttc ggctggttcg gctttaacgg cgggagcgcc ctgggatcgg gcgccctggc
     1741 caccaacgcc ttcgtgacca gccacatcgc ggccggcacg gcgggcctgg tctgggccct
     1801 cctggactgg aaggtcaatt cccacccgac catgctgggg accatcaccg gcgccgtcgc
     1861 gggcctggtg gccatcaccc ccgcggccgg gtttgtggac ccgctcgggg ccctggccat
     1921 cggcgtcacc gtcgcgatca tctgcttcgt gtccgtgatg tacgtcaaag gcaagttcgg
     1981 ctacgacgac tccctggacg ccttcggcgt gcacggcgtc ggcgggttct ggggcgccct
     2041 ggcgacgggg ctctgggcca ccaaggcggt gaacgccgcc ggggccgacg gcctgttcca
     2101 cggcaaccc
//
LOCUS       DAOQLS010000368         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35110_length_2107_cov_4.412281, whole genome shotgun sequence.
ACCESSION   DAOQLS010000368 DAOQLS010000000
VERSION     DAOQLS010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35110_length_2107_cov_4.412281"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PK362_07250"
     CDS             <1..1231
                     /locus_tag="PK362_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74661.1"
                     /translation="ARQTRIYQNQVSTAVAPTAPATLAAAFAFSTTGFSTATFKWDPG
                     SDSGAGATGANTLTYDLEVSTLSSFARETISMASTATPRLGNYFRPPLIHDGNTRHGV
                     VLRSTQPWSGNNAHPGLLTDTTYYFRVRTVDAGLLQSAPSATQTLWTGVPPTTSTLVA
                     VAGTGPGDIVLTWTAPGDDMIYNNLVGNYRIQYSSNAATAWSTSTTPSGAYTATIATS
                     AAVGAAQQTTVTVTTNDTYYIVIWSQDDVGEWSDLSNVANTVPGLILRSVTVTGSPYN
                     FGSLAVGASSVTASGITVTNSGNVPTTYFLEAATTTAGSPWILQPALPAGPDAPVIEG
                     GFHATRPGAGLFGPEDVITNASQSSSASAYSIDGTQTGVSVGIGQVRTLWFKLDMPTT
                     SATSTAQNITVTITANP"
     gene            1284..>2107
                     /locus_tag="PK362_07255"
     CDS             1284..>2107
                     /locus_tag="PK362_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNC74662.1"
                     /translation="MDDSPLRRRTRPIPFGVSRSITVGGWALLAVNSFALPYNAVQTE
                     YENNAGQRLTLGGNGWADMDNDGDLDIVVSGQDSGGNRQFRVYRNNGPAAYTLNATQI
                     EIFGANQGLQDGGVVLGDLNGDGNVDAVVTGNRGAAATRQILVARNNGGLAFTQITVD
                     AGSGLDFGKPALGDFDNDGDLDLLVSGQDPAGQFQLRVYRNNGDATFDPAQIEVPGAA
                     NNGYAGKSSVAWGDYDGDGDLDIVVNGQAPAGRRLHIFRNNGNGTFNATPLDVTAGV"
BASE COUNT          410 a          708 c          611 g          378 t
ORIGIN      
        1 ggctcgccaa acgcggattt accaaaacca ggtctccacc gccgtggccc ccacggcgcc
       61 ggccaccctc gccgccgcgt tcgctttttc aaccacggga ttttcgaccg ccacgttcaa
      121 atgggacccc gggtcggatt ccggcgccgg ggccacgggc gcgaacaccc tgacctacga
      181 cctcgaagtt tccaccctgt ccagtttcgc ccgagaaacc atttcgatgg ccagcacggc
      241 gacgccaagg ctggggaatt atttccgccc ccccctgatc cacgacggga acacccgtca
      301 cggggtggtg ttgcgatcca cccagccctg gtccggcaac aacgcccacc cgggcctgct
      361 gaccgacacg acttattatt ttcgagttcg aaccgtggac gccggtttgc tccaaagcgc
      421 tccgagcgcc acgcagactt tgtggacggg ggttcccccc acgacctcga cgttggtggc
      481 cgtggccggg acgggtccgg gcgacatcgt cctgacctgg accgcgccgg gggacgacat
      541 gatttacaac aatctcgtcg gcaattaccg catccaatat tccagcaatg ccgccacggc
      601 ctggagcacc tccacgacgc cctcgggggc ctacaccgca acgatcgcca cgagtgccgc
      661 ggtcggcgcg gcccagcaaa cgacggtcac ggtgaccaca aacgacacgt attacatcgt
      721 gatttggtcc caagacgacg tgggggagtg gtcggatctc tccaacgtcg ccaacaccgt
      781 gcccgggctg attctccgtt ccgtcacggt gaccgggtcc ccctacaatt ttggctctct
      841 ggccgtcggg gcgagctcgg tcacggcctc gggaatcacg gtgaccaaca gcgggaacgt
      901 cccgaccacg tattttttag aggccgcgac taccacggcc gggagcccct ggattctgca
      961 accggccctg ccggcgggac ccgacgcccc ggtgattgag ggcggctttc acgccacccg
     1021 tcccggcgcc ggactatttg ggccggagga cgtgatcacc aacgcctccc aatcgtcgtc
     1081 cgcctcggct tattccatcg acggaaccca aaccggcgtt tccgttggaa tcggccaggt
     1141 tcgcacgctg tggttcaaat tggatatgcc caccacctcc gccacttcga cggcccaaaa
     1201 catcacggtc acgatcaccg ccaacccgta atttattatc aacccttgaa accctctcca
     1261 aattcgttat cctaatagga tccatggatg actcccccct tcgacgtcgc acaaggccca
     1321 ttccctttgg cgtttcccgc tctataacgg tcggcggctg ggccttgctg gccgtcaatt
     1381 ctttcgccct tccctacaac gccgttcaaa ccgaatacga aaacaacgcc ggccaacggt
     1441 tgaccctggg gggcaatgga tgggccgaca tggacaacga cggcgacctg gacatcgtgg
     1501 tcagcgggca ggattcgggg ggcaatcgcc agttccgggt gtaccgcaat aacgggcccg
     1561 cggcctatac cctcaacgcg acgcaaattg aaatattcgg ggccaatcag ggtcttcagg
     1621 acgggggcgt cgtgttgggg gatttaaacg gcgatgggaa cgtggacgcc gtcgtcaccg
     1681 gcaatcgcgg ggccgccgcg acacggcaaa ttttggtcgc acgaaacaac ggcggtctgg
     1741 ccttcactca aatcacggtg gacgccggat cgggattgga ttttgggaaa ccggccctgg
     1801 gcgacttcga caacgacggg gatttggatc ttttggtgag cgggcaagac cccgccggac
     1861 agttccaatt gcgcgtttac cgcaacaacg gcgacgccac tttcgatccc gcccaaatcg
     1921 aggttcccgg ggccgccaac aacgggtacg ccggcaaaag ctccgtggcc tggggggatt
     1981 acgacggtga tggcgatttg gacatcgtcg tcaacgggca ggccccggcg ggtcggcggc
     2041 ttcacatatt ccgaaacaac gggaacggga ccttcaacgc cacccccctc gatgtgaccg
     2101 cgggggt
//
LOCUS       DAOQLS010000369         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35147_length_2106_cov_3.224768, whole genome shotgun sequence.
ACCESSION   DAOQLS010000369 DAOQLS010000000
VERSION     DAOQLS010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35147_length_2106_cov_3.224768"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..741
                     /locus_tag="PK362_07260"
     CDS             175..741
                     /locus_tag="PK362_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNC74663.1"
                     /translation="MKSKGAWVVLGLVGALAVAGLWAAGTYNRLVGLNEGANAAWAQV
                     ENVLQRRFDLIPNLVNTVKGYAKHEAAVLEEVTRLRSQWGEAKTPGDKIAAAQGLEGA
                     LSRLMVVIEKYPDLKANENFLKLQDELAGTENRIAVERMRFNEAAKAYNVALRVFPGN
                     LVARWAAFAPRAYFEAAPAARTAPVVQF"
     gene            890..1831
                     /locus_tag="PK362_07265"
     CDS             890..1831
                     /locus_tag="PK362_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC74664.1"
                     /translation="MHIETLKIFCDVARLRSFSRSAEVHNVTQSAASQAVHQLESHLG
                     VTLIDRSRRPWTLTPAGKQFYDGCRGLIDQYGALERRVSGRPRTENAEVRVAAIYSVG
                     LRHMREFVEKFSRQNPAVKVHLEYAHPDRVVDAVSEGRADVGIVSFPPTRRDLDVIPW
                     RREEIVVACAPDHPFARRTEVDPARLTGEPFVAFDKDLTVRREMDRFLKKTGVAVEVA
                     LEFDNIEAIKQAVEIGAGVSLLPAPTVEREVRAGALAAIPLKGKPFYRPLGILQRRGN
                     APRPDVAGFVRALRADGAPNEEKPDRGAMSTRTGRKK"
     gene            1828..>2106
                     /locus_tag="PK362_07270"
     CDS             1828..>2106
                     /locus_tag="PK362_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74665.1"
                     /translation="MSEPKQQGLYDPRFEHDACGIGFVVNIKGVRSHQIVADALTVLN
                     NLNHRGASGCEANTGDGAGILIQLPHEFLRTAAGVALPEVGRYGVAMMY"
BASE COUNT          397 a          652 c          698 g          359 t
ORIGIN      
        1 ccctggacgc cctggcgcgg aaaatttcgt tcgacgccga cgttcttcgg gaaatcgcca
       61 acatgaaaac gggggccaag cccgccggag accccgacgt tttgttggac cgctatttga
      121 aggcgatcga cgacgtattg gccgccgtcg acgccctgtg accgggagga atcgatgaaa
      181 agcaaaggcg cgtgggtcgt tttggggttg gtgggggcgt tggccgtggc cgggctgtgg
      241 gccgcgggga cgtacaaccg gttggtgggg ctcaacgagg gcgccaacgc cgcctgggcc
      301 caggtggaaa acgttttaca gcggcgtttc gatttgattc ccaacctggt caacaccgtg
      361 aaaggctacg ccaagcacga agcggcggtg ctggaggaag tgacgcggct tcgaagccaa
      421 tggggggagg ccaaaacccc gggggacaag atcgccgccg cccagggact ggaaggcgcc
      481 ctgtcgcgac tcatggtcgt gatcgaaaaa tacccggacc tcaaagccaa cgagaatttt
      541 ctgaaattgc aggacgagct ggcggggacg gaaaaccgga tcgccgtcga gcgcatgcgt
      601 ttcaacgagg ccgcgaaggc ctacaacgtg gccctgcgcg tttttccggg gaatctcgtg
      661 gcccggtggg cggccttcgc cccgcgcgcc tatttcgagg cggcgcccgc ggctcggacc
      721 gccccggtgg ttcaattttg agggcggttt ctttatatta gtatttctaa tattgaaatc
      781 ttttaatatt cgataaaaaa atatagcatc tctcgaccgg tctccggccc cttgggcggg
      841 gccccggccg gcgcgcggag cgcccgattc gttgaccctc gggggacgcg tgcacatcga
      901 aacactgaag atcttttgcg acgtggcccg cctgcgcagc ttctcccgct ccgccgaggt
      961 tcacaacgtc acccaatccg ccgccagcca ggccgtgcat cagttggaat cccacctggg
     1021 cgtgacgctc atcgaccggt cccgacgtcc ctggacgctc acccccgccg gaaagcaatt
     1081 ttacgacggt tgccgagggt tgatcgatca atacggcgcc ctggaacggc gggtgagcgg
     1141 ccgtccgcgg acggaaaacg ccgaagtgcg cgtggcggcc atctattccg tgggcctgcg
     1201 ccacatgcgc gagttcgtgg aaaaattttc aaggcagaac ccggcggtca aagtgcattt
     1261 ggaatacgcc catccggacc gggtggtcga cgcggtgagc gagggccggg cggacgtggg
     1321 gatcgtttcc ttccccccga cccggcggga tctggacgtg atcccctggc gccgggagga
     1381 gatcgtcgtc gcctgcgccc cggaccaccc cttcgcccgg cggaccgagg tggaccccgc
     1441 gcggttgacg ggggagccct tcgtcgcctt tgacaaggat ttgacggtcc gccgcgaaat
     1501 ggaccggttt ttgaaaaaaa ccggcgtcgc ggtggaggtc gccctcgagt ttgacaacat
     1561 cgaggcgatc aagcaggcgg tggaaatcgg cgcgggcgtg tctctgctgc cggcgcccac
     1621 cgtcgagcgg gaggttcggg ccggcgccct ggccgcgatc ccgctcaagg gcaaaccgtt
     1681 ttaccgcccc ctgggcattc ttcagcgccg agggaacgcc ccgcgccccg acgtcgcggg
     1741 gttcgtgcgg gctttgcggg cggacggcgc gccgaacgaa gaaaagccgg accgcggcgc
     1801 catgtcaacg aggacaggac gaaaaaaatg agcgaaccca aacagcaggg cctatacgat
     1861 ccccgattcg agcacgacgc ctgcggcatc ggttttgtcg tgaacatcaa aggggtccgg
     1921 tcccaccaaa tcgtcgcgga cgcgctgacc gtcctgaaca atttaaacca ccgcggcgcc
     1981 tccggctgcg aagccaacac cggcgacggc gccgggatcc tgatccaatt gccccacgaa
     2041 ttcctgagaa cggcggccgg cgtggccctg cccgaggtcg gccgttacgg cgtggccatg
     2101 atgtat
//
LOCUS       DAOQLS010000370         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35148_length_2106_cov_3.219405, whole genome shotgun sequence.
ACCESSION   DAOQLS010000370 DAOQLS010000000
VERSION     DAOQLS010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35148_length_2106_cov_3.219405"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PK362_07275"
     CDS             <1..436
                     /locus_tag="PK362_07275"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74666.1"
                     /translation="TGFPWLPLACTQWLVPKHLPLAALGGVYAVSFLVALFNGALALL
                     LDGRLRGEPRWARPAGPAFLALAGLTALSVALWRHPPAEGPALRAAIVQGNIDQYKKW
                     DQSYEDEIVAAYTGLTQTAAAAGPALIVWPETAVPGWIPNDE"
     gene            449..1120
                     /gene="lnt"
                     /locus_tag="PK362_07280"
     CDS             449..1120
                     /gene="lnt"
                     /locus_tag="PK362_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNC74667.1"
                     /translation="MAGLAKAARIPILFGAVSRQSGRDYNAAFLMSPAGELIGQYRKR
                     HLVPFGEYAPFQKILGRLDGVLNSLGSFDAGEDAGVVPGPVPLGVSVCFEGLFPGLVA
                     DFSRAGAKVLVNITNDGWYRDTAAPEQHFAANVLRAVENGRWVLRAANTGLSGFINPR
                     GEVIEKSELLTAAVIQGPLVPADRETFYVRHGDWFAGACYLIVLAGAVGFFHKNRFFR
                     RVPAS"
     gene            complement(1152..1997)
                     /locus_tag="PK362_07285"
     CDS             complement(1152..1997)
                     /locus_tag="PK362_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC74668.1"
                     /translation="MEKSILLTGASGGFGRCLVAELHARGHRVLAALRGGENRGESLF
                     PDLVDSPRLKFADLDLCDPASIHHLVEKTKLHFGKQLDVLINNAGAAVLGPVEHQSLT
                     DVRRQFDLNFFGPLALLQALLPVLRSSRGRILNVTSMTAYATFPFYGTYSASKHALDA
                     ATEALYYDLAEAGVQVCAVEPGAYRTGFTPAALRPALPEGAPALDKRRVRAFEGFLQS
                     LRETEKDPRAVARRLADLCESRRIPPRARIGRDAHFFWFLRRWMPDGWWVRGMEWFFR
                     RRVFR"
BASE COUNT          361 a          665 c          711 g          369 t
ORIGIN      
        1 gacgggtttt ccctggctac ccctggcctg cacccagtgg ctcgttccca aacatctgcc
       61 cctggcggcc ctcggcggcg tgtacgcggt gtcgttcctc gtggcgctct tcaacggagc
      121 cctggccctg ttgttggacg gacggcttcg aggggaaccg cggtgggcgc ggcccgccgg
      181 gcccgctttt ttggccttgg ccggtttgac ggctctgagc gtggcccttt ggcgtcatcc
      241 cccggccgag ggccccgcgc ttcgggccgc catcgtccag ggaaacatcg accagtacaa
      301 aaagtgggac caaagttacg aggacgaaat cgtggccgcc tacaccgggc tcacccaaac
      361 ggcggccgcc gccggcccgg cgctcattgt ctggccggaa acggcggtcc cgggctggat
      421 tcccaacgac gagtaatacc tgaaatgggt ggccggcctg gccaaggcgg cccggattcc
      481 gattttgttc ggggccgttt cgcggcaatc ggggcgggat tacaacgcgg cgttcttaat
      541 gtcccccgcg ggggaattga tcggccaata ccgcaagcgg cacctggtcc ccttcgggga
      601 atacgcgccc tttcagaaaa tcctcggccg gttggacggg gtgctcaaca gtttgggatc
      661 cttcgacgcg ggggaggacg ccggggtggt cccgggtccg gtaccgttgg gcgtgagcgt
      721 ttgctttgag gggctctttc cggggttggt cgcggacttt tcccgggccg gggcgaaggt
      781 cctggtgaac atcaccaacg acggctggta ccgggacacg gccgcgccgg aacagcattt
      841 cgccgccaac gtgctccggg ccgtggagaa cggccggtgg gtcctgcggg ccgcgaacac
      901 gggcctttcg gggttcatca accctcgggg cgaggtgatt gaaaaatcgg aactcctcac
      961 ggcggcggtg attcagggcc cgttggtccc ggcggaccgg gaaacctttt acgtccgcca
     1021 cggggattgg tttgccgggg cctgctattt gattgtcctg gccggggcgg tcgggttctt
     1081 ccacaagaac cgcttcttcc ggcgcgtccc cgcctcctaa acgttaccgc cggcccgccg
     1141 gcggccgcgc gtcagcgaaa tacccgcctt cggaaaaacc attccattcc ccgaacccac
     1201 cagccgtccg gcatccagcg tcggagaaac caaaaaaaat gggcgtcccg cccgatccgc
     1261 gcgcgggggg ggatccgccg ggattcgcaa agatcggcca accgtcgggc caccgcccgg
     1321 gggtccttct ccgtttcccg aagcgattgt aaaaatccct caaaggcccg aacgcgccgt
     1381 ttgtccaggg cgggcgctcc ttcgggaagg gccggtcgaa gggccgccgg ggtgaacccg
     1441 gtccgatagg cgccgggctc caccgcgcag acctgcaccc cggcttccgc cagatcgtaa
     1501 taaagcgcct cggtggcggc gtccagggcg tgtttggagg cgctgtaggt gccgtaaaaa
     1561 gggaaggtcg cgtaggccgt catactggtc acgttgagga tccgcccccg ggaggaccgc
     1621 aagacgggca ggagggcctg aagaagggcc agagggccga agaaattcag gtcaaactgg
     1681 cggcggacgt ccgtcaggga ctggtgctcg acggggccca gcaccgccgc cccggcgttg
     1741 ttgatcagca cgtccaattg ctttccgaaa tgaagttttg ttttttccac cagatggtgg
     1801 atcgaggccg ggtcgcacaa atcgagatcg gcgaatttga gtcgggggga atcgaccaaa
     1861 tccgggaaaa gggactcgcc ccggttttcg ccgccccgca gggccgccag cacccggtgg
     1921 ccccgggcgt gcagttccgc caccaggcag cggccaaaac cgcccgaggc gccggtcagc
     1981 aggatcgact tttccattcg ttaagagtat aggggaggaa cgcccaaaaa aaaagacttt
     2041 cgggcccgcc ccggcggatc gttttcgggt tgaatttgcc ccgcgggggg atatactgaa
     2101 accatg
//
LOCUS       DAOQLS010000371         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35335_length_2100_cov_4.005379, whole genome shotgun sequence.
ACCESSION   DAOQLS010000371 DAOQLS010000000
VERSION     DAOQLS010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35335_length_2100_cov_4.005379"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PK362_07290"
     CDS             complement(<1..141)
                     /locus_tag="PK362_07290"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNC74669.1"
                     /translation="MPHLGVNIDHVATLRQARRGESPDVVAAARAARAGGADSLTVHL
                     RED"
     gene            222..1706
                     /locus_tag="PK362_07295"
     CDS             222..1706
                     /locus_tag="PK362_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74670.1"
                     /translation="MTLREKGAALLRRPAFWLSVFVLTALGLLPMGYIGQDHDDALYF
                     LAAQSLREGRYRHWFLPGSPPITNLTPGFPAFLAPIAFLFPDNALACQLWAGLALLAC
                     TGAVWLFFRRRQDAPTAVLLTGLFALNPLVLGRLGVVMPEAPFLLGALGFFLLWEKNR
                     LPDFAAGAWLSVLYLVRPAALPLWGAVGASLLLQRRFRSLLKTFAIPALTYFLWARWS
                     GQGGGVEESVELNLLYGGKGPGQWFLNAGLNAAQLARTWGATVLPVGWARGGAAPWVG
                     AVLLLLAVVGLARILRRIPADVPMGFLALSLGMHLAWPWWYDRYLLPLLPFLWLGLAA
                     TAADLDRRFSPRVRRGLLGGVLVLSFVGQSVFWFDPAARRNTPELADSYAWIQSHTSP
                     ADGFMSLFYGRDLIHTQRVFLPPPPAEDAAALDAALAQRRVRYVLWDRTIQLGFSDGR
                     NPVTSAVDRLTRSLEDPARFRLVHEDTSGRARIYERRKGRAGTN"
     gene            complement(1703..>2100)
                     /locus_tag="PK362_07300"
     CDS             complement(1703..>2100)
                     /locus_tag="PK362_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNC74671.1"
                     /translation="EGMKKTGAVLGGEPSGHVIFRDFLPTGDGLLTALQVLTVLAETG
                     RPLSALASLGVRYPQVLLNVPVKERRPLDRVPGFAAALAAVQRALGSTGRTLIRYSGT
                     EPLLRVMVEGPDHAAIERHAQVLARAAVR"
BASE COUNT          277 a          713 c          688 g          422 t
ORIGIN      
        1 gtcttctcgc agatgaaccg tgaggctgtc ggccccgccg gcccgggccg cccgagcggc
       61 cgccacgacg tcgggcgact ccccgcgccg ggcctgccgc agggtcgcca cgtggtcgat
      121 gttgactccc aaatggggca ctttttgtcc gtctttcagt cggtgaatgt gaaaagtcta
      181 ttatttcaaa tccgtggtcg tttcgctatg attctcgaac catgacgctc cgcgaaaaag
      241 gggccgccct gcttcggcgg cccgcctttt ggttatcggt tttcgtcctc acggccctgg
      301 ggctccttcc catggggtac atcggccagg atcacgacga cgccctctat ttcctggcgg
      361 cccagtccct gcgggaaggc cgttaccggc actggttttt gccgggctcc ccgcccatca
      421 ccaacctcac cccgggattt ccggcctttc tggcgccgat cgccttccta ttcccggaca
      481 acgcgctggc ctgccaactg tgggcgggcc tggcgctcct ggcctgcacg ggcgccgtgt
      541 ggctgttttt tcggcgccgc caggacgcgc ccaccgccgt cctcctgacc ggcttgttcg
      601 ccctgaaccc gctggtcctg gggcggctcg gcgtcgtgat gcccgaggcg ccttttttgc
      661 tgggcgccct cggattcttc cttttgtggg agaaaaatcg attgcccgac ttcgccgctg
      721 gggcctggct gtcggtcctc tacttggtcc gtcccgcggc ccttcccctc tggggcgcgg
      781 tgggggcgag ccttctgctt caacgtcggt tccggtcgtt gctcaaaacc ttcgctattc
      841 cggcgttgac ttattttctg tgggcccgtt ggtcggggca ggggggcggc gtggaggaat
      901 ccgtcgaatt gaacctcctt tacggcggga aaggcccggg gcaatggttt ctgaacgcgg
      961 ggctgaacgc ggcccaattg gcccgcacct ggggcgcgac cgttttgccg gtgggctggg
     1021 cccggggcgg ggcggccccg tgggtcggcg ccgtccttct ccttttggcc gtcgtcggcc
     1081 tggcgcgaat ccttcgacga atacccgccg acgtcccgat gggatttttg gcgctcagcc
     1141 tcggcatgca cctggcctgg ccctggtggt acgaccgcta tctgcttccc ctgctccctt
     1201 ttctttggtt gggcttggcg gcgacggcgg cggatctgga ccgtcgtttt tcaccgcggg
     1261 ttcggcgggg gctgttgggc ggggtcctcg tcctgtcctt cgtcggccaa agtgtttttt
     1321 ggttcgaccc cgccgcccgg cggaacacgc cggaattggc cgacagctac gcctggatcc
     1381 aatcccacac gtcgccggcc gacggattca tgagcctctt ttacggacgg gatttgatcc
     1441 acacccagcg cgtgttcctt ccgcccccgc cggcggagga cgccgcggcg ctggacgcgg
     1501 ccctggccca acggcgcgtg cgctacgtgc tttgggatcg aacgatccaa ttggggtttt
     1561 ccgacggacg gaaccccgtc acgtcggcgg tggaccgtct gacccggtcc ctggaagacc
     1621 cggcgcgatt tcgcctcgtt cacgaggaca cgtcggggcg ggcccgaatt tacgagcgtc
     1681 ggaaagggcg ggcggggacg aactagcgga cggcggcccg ggccaagacc tgggcgtgac
     1741 gttcgatggc ggcgtgatcc ggaccctcga ccatcacccg gaggagcggc tcggtccccg
     1801 aatagcggat caacgtgcgt cccgtggacc ccaacgcccg ctgcaccgcc gccagggcgg
     1861 cggcaaatcc gggcacacgg tccaagggac ggcgttcctt gacgggaacg ttgaggagca
     1921 cctggggata ccgcaccccc agggacgcca gggccgacag gggccggccc gtttccgcca
     1981 aaaccgtcaa gacctgaagg gccgtcaaca acccgtcgcc cgtggggaga aaatcccgga
     2041 agatgacgtg ccccgacggc tctccgccca ggacggcccc ggtttttttc atgccttccc
//
LOCUS       DAOQLS010000372         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35392_length_2098_cov_3.729809, whole genome shotgun sequence.
ACCESSION   DAOQLS010000372 DAOQLS010000000
VERSION     DAOQLS010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35392_length_2098_cov_3.729809"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK362_07305"
     CDS             <1..288
                     /locus_tag="PK362_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74672.1"
                     /translation="AKPDVDGDVSEPALSSGDLILVCSDGLSRMVKDAAIAGELRRLK
                     EPTEMCHNLVRLALEQGGRDNVTVAVARARGGGFWSSVARVFRRKPAAHAG"
     gene            310..1011
                     /locus_tag="PK362_07310"
     CDS             310..1011
                     /locus_tag="PK362_07310"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC74673.1"
                     /translation="MAKLLLKFNGAILKELPIVLPETTIGRRPDCDLVIDNPAVSGHH
                     AKVLMEPTGAFFIEDVGSTNGTYLRDRKVTKSPLHHEDELVIAKHTVTFINEEEAARA
                     AAAAAPRETVSSDATIVMSSPPPPPKITADKVGFLRAVEGDSPVEPLALTQLTTYIGK
                     SAQAHIKLKGFFQPDLACCIVKKAEGHSLTSLKDKVVKLNGDPLMDQAQVPLKEGDVI
                     NVGGLKLLYFLQDAP"
     gene            1083..>2098
                     /locus_tag="PK362_07315"
     CDS             1083..>2098
                     /locus_tag="PK362_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNC74674.1"
                     /translation="MRSLHGLYRGIGFLSTLLVFGAVAGPASALNVRRIDGRTEIKRS
                     GRTEWRPVDDSTSVREGDTLRTGPKGKAELRMSEGHRLSVREKTTVTVVSGGKGLQYQ
                     FRVISGRVRAFVRKLAAKSRFEIRTPLAVAAVRGTEFEMEVLDRGTSRLSVIEGVVSF
                     KDLDGLFNEVSVVKGQSVTIEPNQAPKPPEPVPQDILKEKSAEAAVSPPGPPKEEIER
                     EAGLDGDRDFRQEQGANNLKEGVYQEGKALIDAYGKRVRLEEYVLRPTATQFAFVAVS
                     RRDHRTDATRLDIFAQNPLPDVVDVKGLFYQEGNSNMLNWAVATQRIATNGGDWVKEW
                     RSDGT"
BASE COUNT          437 a          644 c          627 g          390 t
ORIGIN      
        1 gccaaacccg acgtggacgg ggacgtgtcg gaaccggcgt tgtcgtcggg cgatttaatt
       61 ttggtgtgct cggacggtct ttcccgcatg gtcaaagacg cggccatcgc gggggaactc
      121 cgccgtttga aggagccgac ggaaatgtgc cataatctcg ttcgactcgc cctggagcaa
      181 ggcggacgcg acaacgtcac cgtggccgtc gcgcgggccc ggggcggggg gttctggtcc
      241 tcggtggcac gggttttccg gcgaaaaccg gcggcgcacg ccggctgacc gatacgaagg
      301 ggcgacaacg tggccaaact tctcttaaag tttaacgggg cgattctcaa ggaattgccc
      361 atcgttcttc cggaaaccac catcggccgg cggcccgatt gcgatctggt catcgacaac
      421 cccgccgttt ccggccatca cgccaaagtc ctcatggaac ccaccggcgc cttcttcatc
      481 gaagacgtcg ggagcaccaa cggaacctac cttcgggacc ggaaggtcac gaaatcgccc
      541 ctccatcacg aagacgaatt ggtcatcgcc aagcacacgg tcacttttat caacgaggaa
      601 gaagccgccc gggccgcggc cgcggcggcg ccccgcgaga cggtttcctc cgacgccacc
      661 atcgtcatgt cgtccccgcc gcctccgccg aaaattaccg ccgacaaagt cggtttcctg
      721 cgcgcggtgg aaggggactc tcccgtggaa cccctggccc tcacccaatt gaccacctac
      781 attgggaaat cggcccaggc ccacattaaa ttaaaggggt ttttccagcc ggatttggct
      841 tgttgcatcg ttaaaaaagc cgaaggtcat tccctcacgt ccctgaaaga taaagtcgtc
      901 aaattgaacg gggatccttt aatggatcag gctcaggtcc cgctcaaaga aggcgatgtg
      961 atcaacgtgg gcggccttaa attgctttat ttcctccagg acgctcccta gccttttttc
     1021 ggtttcaccg cccgccccgc gccccttgac tttccatcca tgggggctat atccttgaag
     1081 caatgagaag cctgcacgga ctttaccgtg gaatcggctt tctttcgacg cttctggttt
     1141 ttggagccgt cgccggaccg gcgtcggctt taaacgttcg gcggatcgac ggtcgaaccg
     1201 aaattaaacg ttcgggccga accgagtggc gacccgtgga cgattccacc tccgtgcggg
     1261 agggcgatac cctccgcacg ggccccaagg gaaaagccga actccgaatg agcgagggtc
     1321 accggctttc ggttcgggaa aagacgaccg tgacggtggt ttccgggggg aaggggcttc
     1381 aataccaatt tcgcgtcatc tccgggcggg tccgggcttt cgtgcgaaaa ctcgccgcca
     1441 aaagccggtt tgaaatacgc actcccctgg ccgtggccgc cgtccgcggc acggaatttg
     1501 aaatggaggt gctggaccgg ggcacctcgc gactttcggt catcgaaggc gtcgtgtcgt
     1561 ttaaggacct ggacggtctt tttaacgaag tgagcgtcgt caaaggccaa agcgtgacca
     1621 tcgaacccaa tcaggccccc aaacccccgg agccggtccc ccaggacata ttgaaggaaa
     1681 aatccgcgga agcggccgtt tcgcccccgg gcccccccaa ggaagaaatt gagcgggagg
     1741 ccgggctgga cggggaccgg gatttccgtc aagagcaggg cgccaataat ttgaaggaag
     1801 gcgtttacca agaggggaaa gccctcatcg acgcctacgg caagcgcgtg cgcctggaag
     1861 aatacgtcct gcgaccgacc gccacgcaat tcgcctttgt ggccgtgagc cgacgggatc
     1921 atcgcaccga cgccacccgc ctggacattt tcgcccaaaa ccccctgccg gacgttgtcg
     1981 acgtcaaagg cttgttttat caggaaggga actccaacat gctcaactgg gccgtggcca
     2041 cgcagcggat cgccaccaac ggcggggatt gggtgaagga gtggcggtcc gacgggac
//
LOCUS       DAOQLS010000373         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35395_length_2098_cov_3.419971, whole genome shotgun sequence.
ACCESSION   DAOQLS010000373 DAOQLS010000000
VERSION     DAOQLS010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35395_length_2098_cov_3.419971"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK362_07320"
     CDS             <1..489
                     /locus_tag="PK362_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNC74675.1"
                     /translation="FSRPDFDSYFMGIAQAVCRRADCRGSRVGAIIVVDRRIVSTGYN
                     GTAEGMPNCTDGGCERCLNRDKYPKGTGYDLCICVHAEQNALLAAARFGIAVAGGTAY
                     TTLQPCFGCLKEMLQAKIRRVCYIDAWSHPDPKFAEQYNRIAARLPGGMARVEPPAPI
                     AG"
     gene            486..935
                     /locus_tag="PK362_07325"
     CDS             486..935
                     /locus_tag="PK362_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74676.1"
                     /translation="MIPLRPAAVVWGATLLLSPALRAQDDPGVLLEGPAGARALAAVT
                     DRTSPGVPDPEILFDEKGRPFVSTTHFIVVLKPRATVGQANAALKSMNARVVGMVKGQ
                     DVMEIRLPDPQRRRTLRDVDALVAALRRLPPFKDALPFSFQNAAEAP"
     gene            946..>2098
                     /locus_tag="PK362_07330"
     CDS             946..>2098
                     /locus_tag="PK362_07330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC74677.1"
                     /translation="MIIDPGSFRDPRGRVFTHEGRVFRALSSEGWAAWRQFQGSALFD
                     RLSQSGRVIETREAAGIPLPPGEWAGVLEHAPVPCLSYPYEWPFGALRDAALFFLELL
                     SETLREGFGLRDATPYNIQFRGIRPVHIDLPSFERRGPDGGWNGYGDFCRSFLYPLLL
                     EAHKGIPFQRWVRGTLEGFSPLEMARLFGWRDWAKAGVFKDVVLQAALGPWGERLDPR
                     AFQNDLRRALPLASVLKNIDRLQKIVAGLRSGAPAGPWRNYRRTHPYSEADRRERTRF
                     VDNALSPRRRCVWDLGSNDGEFAIRAARSAGNVLALDADSTAVEDLRRRLAREGVGNV
                     LPLVVDLADPSPAQGWRGRERRRLEDRSSPDLVLCLALTHHLVISRNIPL"
BASE COUNT          305 a          754 c          676 g          363 t
ORIGIN      
        1 ttttcccgac cggatttcga ctcgtacttc atgggcatcg cccaggcggt ttgccgacgg
       61 gccgattgcc gcggcagccg ggtgggcgcc atcatcgtgg tggaccgccg catcgtctcc
      121 acgggctaca acggcaccgc cgaaggcatg cccaattgca ccgacggcgg ctgcgaacgc
      181 tgcctgaacc gggacaaata ccccaagggc acgggctacg acctgtgcat ctgcgtccac
      241 gcggagcaaa acgcgctgct ggccgcggcg cggttcggca tcgccgtcgc gggcggcacc
      301 gcctacacca cgcttcaacc ctgcttcggc tgtttgaagg aaatgctcca ggccaaaatc
      361 cggcgggtct gttacatcga cgcctggtcc caccccgacc cgaaattcgc cgaacaatac
      421 aaccgcatcg ccgcgcggct gcccggcggc atggcccggg tggaaccccc cgctccgatc
      481 gccggatgat cccccttcgg cccgcggcgg tggtttgggg ggcgacgctg ctcctctccc
      541 ccgcgctccg ggcccaggac gacccgggcg ttttgctcga gggcccggcc ggggcccggg
      601 cgctggccgc cgtgaccgac cggacgtccc ccggcgtccc cgacccggaa atcctcttcg
      661 atgaaaaggg gcggccgttc gtctccacca cccatttcat cgtcgtcctc aagccccgcg
      721 ccaccgtggg ccaggcgaac gccgccctca aatccatgaa cgcccgggtc gtcggcatgg
      781 tcaaggggca ggacgtaatg gaaatccgcc tgcccgaccc ccaacgccgc cggaccctcc
      841 gggacgtcga cgccctggtc gccgccctcc gccgcctgcc ccccttcaaa gacgccctgc
      901 ccttttcctt ccagaacgcc gccgaggccc cgtgagcggg cgtcaatgat catcgacccc
      961 ggttcctttc gggacccccg ggggcgcgtg tttactcacg aagggcgcgt tttcagggcc
     1021 ttgtcctcgg agggttgggc ggcctggcgg caatttcagg ggtccgctct cttcgaccgg
     1081 ctctcccaat ccggtcgcgt gatcgagacc cgggaggcgg cgggaatccc ccttcccccc
     1141 ggcgagtggg ccggggtctt ggaacacgcg ccggtgccct gcctgtccta tccctacgaa
     1201 tggcccttcg gcgccctccg ggacgccgcg cttttttttc tggaattgct gtcggaaacc
     1261 ctgcgcgagg gctttggcct ccgcgacgcg acgccctaca acatccaatt ccgagggatt
     1321 cgcccggtcc acatcgacct tccgtccttc gagcgtcggg gccccgacgg aggatggaac
     1381 gggtacgggg atttttgccg ttcctttctt taccccctgt tgctcgaggc ccacaaagga
     1441 atcccgttcc aacgatgggt tcgggggacc ctcgaaggtt tttcccccct cgagatggca
     1501 cggcttttcg gatggcggga ttgggcgaaa gccggcgtct tcaaagacgt cgtcctgcaa
     1561 gccgccctgg gcccctgggg cgaacgcctg gatccccggg cgtttcaaaa cgatctgcgc
     1621 cgcgctctcc ccttggcgag cgttttgaaa aacatcgacc ggcttcaaaa aatcgtcgcg
     1681 ggactgcgtt ccggagcccc cgcgggcccc tggcggaact accggcggac ccacccttac
     1741 tcggaggcgg accgtcggga acggacgcgg tttgtcgaca acgcgttgag cccgcgccgc
     1801 cgctgcgttt gggatttggg ttccaacgac ggggagttcg cgattcgggc ggcgcgatcc
     1861 gccgggaacg tgctggcgct cgacgcggat tcaacggccg tggaggatct ccgccggcgc
     1921 ctggcccggg aaggggtggg gaacgtcctg ccgttggtcg tggacctggc ggacccctcg
     1981 cccgcccagg ggtggcgggg gcgcgagcgc cggcgcctgg aggaccggtc gtcgccggac
     2041 ctggtgctgt gcctggcgct gacgcaccac ctggtgattt cgcgaaacat cccgcttc
//
LOCUS       DAOQLS010000374         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35478_length_2095_cov_4.005392, whole genome shotgun sequence.
ACCESSION   DAOQLS010000374 DAOQLS010000000
VERSION     DAOQLS010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35478_length_2095_cov_4.005392"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="PK362_07335"
     CDS             complement(<1..149)
                     /locus_tag="PK362_07335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNC74678.1"
                     /translation="MASLRELRRKVKSVKSTQQITKAMKMVAAARLRRGQARILGARP
                     FANKMA"
     gene            complement(159..1673)
                     /gene="atpA"
                     /locus_tag="PK362_07340"
     CDS             complement(159..1673)
                     /gene="atpA"
                     /locus_tag="PK362_07340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC74679.1"
                     /translation="MAIQPEEITRVLKERLEGFQLTAEMKEVGTVLQVGDGIARVWGL
                     ANALAGEMLKFPHGVVGIVLNLERESVGAVLLGESEKIREGDKVERTGRIMEVPVGEA
                     LIGRVVNALGQPIDGKGPLNTKKARPIEVVAPGVVERQPVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAIDTIVNQKNDPNRPVCIYVAIGQKQSTVAQVVQKLTDLG
                     AMDYSIVVSASASEAAPLQFIAPYAGCAIGEEFMWNGRAVLCVYDDLSKHAVAYRQLS
                     LLLRRPPGREAFPGDVFYLHSRLLERACKLSDKNGGGSLTALPVIETQAGDVSAYIPT
                     NVISITDGQIYLESNLFFSGVRPAVNVGLSVSRVGGSAQVKAMKQVAGKLRLDLAQYN
                     ELAAFAQFGSDLDKASQAQLARGQRIVEILKQDQYQPQSVPRQVALIFAATNGFLDDL
                     PVEALRRFEADLLNWLEKDRGDLLKTLADKKTLDDDLKAKLSAAVGEFKARFKV"
     gene            complement(1684..>2095)
                     /gene="atpH"
                     /locus_tag="PK362_07345"
     CDS             complement(1684..>2095)
                     /gene="atpH"
                     /locus_tag="PK362_07345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNC74680.1"
                     /translation="AAALQHPRVAPAVKGELLARALGKPTAFTGRLLDLLMEKKRLDA
                     LGEIARRFNEMADEARGVVAVAVGTAQPLTAEQTASLKKSLSKIFGDVTLHPRADPAL
                     LGGLVVQVGDRLWDGSVAAQLARLKDAWLSSATN"
BASE COUNT          342 a          671 c          681 g          401 t
ORIGIN      
        1 gccattttgt tggcgaaggg ccgggccccc agaatgcggg cctgcccccg gcgcagacgg
       61 gccgcggcca ccatcttcat ggccttggtg atctgctgcg tcgatttgac gcttttgacc
      121 tttcgccgga gctcccgaag ggacgccatg gggaatcgtt aaaccttgaa ccgcgcctta
      181 aattccccga cggcggccga caacttcgcc ttgagatcgt cgtccagggt tttcttgtcg
      241 gccagggttt tcaacagatc gccccggtcc ttttccagcc agttcaagag gtccgcttcg
      301 aaccgacgca gggcctccac cggcaggtcg tccaggaagc cgttggtcgc ggcgaaaata
      361 agcgccacct ggcgcggcac ggactggggc tggtattggt cctgcttcaa tatttcgacg
      421 atgcgctgcc cccgggccag ctgggcctgg gaggccttgt cgaggtccga accgaattgg
      481 gcgaaggccg ccagctcgtt gtactgggcc aggtccaagc ggagtttgcc ggccacctgc
      541 ttcatggcct tcacctgggc gctgccgccc acgcggctga cggaaagccc cacgttgacc
      601 gcgggccgga cgccggagaa gaacaggttc gactccaggt aaatctgccc gtcggtgatg
      661 gaaatcacgt tggtcgggat atacgcggac acgtcgccgg cctgggtctc gatgacgggc
      721 agggccgtaa gggacccgcc gccgtttttg tccgacaatt tgcaggcgcg ctccaacaac
      781 cgggaatgca ggtagaagac gtccccgggg aacgcctcgc gccccggcgg ccggcgaagc
      841 agaagcgaca gctggcggta ggcgacggcg tgtttggaca aatcatcgta aacgcaaagg
      901 accgcgcggc cgttccacat gaattcctcg ccgatggcgc agccggcgta gggggcgatg
      961 aattggaggg gcgcggcttc ggacgccgag gcgctcacca cgatggaata gtccatggcg
     1021 cccaaatccg tcaatttctg caccacctgc gcgacggtgg attgcttttg cccgatggcc
     1081 acgtaaatgc agacggggcg gttcggatcg ttcttttggt tgacgatggt gtcgatggcg
     1141 atggccgttt tacccgtctg ccggtcgccg atgatcagtt cccgctggcc gcggccgatg
     1201 gggatcatgg cgtcgatggc cttgatgccg gtctgaaggg gttcgcgcac gggctgccgt
     1261 tccacgacgc cgggggccac gacctcgatg ggccgggctt tcttcgtgtt gagcgggccc
     1321 ttcccgtcga tgggttggcc gagggcgttg accacgcggc cgatcagggc ctcgcccacg
     1381 ggcacttcca tgatgcgtcc cgtgcgctcc accttgtcgc cttcccgaat tttttccgat
     1441 tcgccgagca ataccgcgcc cacgctttcg cgttcgaggt tcaaaacaat gcccaccacg
     1501 ccgtggggaa acttcagcat ttcgccggcc agagcgttgg ccaggcccca gacgcgggcg
     1561 atgccgtcgc cgacctggag gacggtgccg acttccttca tttccgccgt gagttgaaag
     1621 ccttccaggc gctctttgag aacgcgggtg atttcttcgg gttggattgc catggggtat
     1681 cctttaattc gtggccgaac tcagccacgc gtctttcaat cgagccaatt gcgccgccac
     1741 gctgccgtcc cagaggcggt cgccgacctg aacgaccagt ccccccagga gcgcggggtc
     1801 cgcgcggggg tgaagcgtca cgtcgccgaa aatcttggac agcgatttct tgagcgacgc
     1861 cgtctgctcg gcggtcagcg gctgggccgt gcccaccgcc accgccacca cgccccgggc
     1921 ttcatcggcc atttcgttga aacggcgggc gatctcgccc agggcgtcca ggcgtttctt
     1981 ttccatcaaa aggtccaaca accggccggt gaaggccgtg ggtttcccca gggcccgggc
     2041 cagcaattcg cctttgaccg cgggagcgac ccgcgggtgc tgcagggccg cggcc
//
LOCUS       DAOQLS010000375         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35638_length_2090_cov_3.417199, whole genome shotgun sequence.
ACCESSION   DAOQLS010000375 DAOQLS010000000
VERSION     DAOQLS010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35638_length_2090_cov_3.417199"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1748
                     /locus_tag="PK362_07350"
     CDS             36..1748
                     /locus_tag="PK362_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNC74681.1"
                     /translation="MTSEVPVKDDMSMEELLAEAEAQSRPGSLVTAHVVEVTPAGVLV
                     DVGLKVEGLIPLAEFRSQAQPPKVGDSFPALIKRLSGPEGHPLVSFKEARDRSQWTHV
                     IQARDAGTPLEATVIRAVKGGLIVDVGMEAFLPASQVDRRPVKDLTPLVGQKFPVAVL
                     EMDTAKGNVVVSRRKLLEKDASQKRADTLKTMEVGKVFKGTVTSLTAFGAFVDIGGIE
                     GLLRITDVSWGWVGKLSDVLKAGDVVDVKVLKFDPASGKIALGRKQLLAKPWDSVDAK
                     FPVNSIQKGKVTSLTDFGAFVELAPGVEGLVHQSEFSWKERGVKPKELLTVGQEVYVF
                     ILSINKEQEKMALSLKRAGENPWVEAAREYPAGSRVKGVVTHMLPVGAFVRLPSGIEG
                     LLRTQDMSWTKAPAHPKDLLTVGQEIEAVVIDVNPTTEKMALGLKQLTEDPIAKFAPG
                     ATVEGKVVRLTDQGAFLELAPDIEGFVHISEIPSDHRLNHAGEVLKEGETVTGLVLKG
                     QRKSRRIELSIRKHDQREERQLLKRYRPSGEGVTLGDVTEWGSADESAAGEPPASDSP
                     TPNA"
     gene            1798..>2090
                     /locus_tag="PK362_07355"
     CDS             1798..>2090
                     /locus_tag="PK362_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74682.1"
                     /translation="MSFDPLIPAFLLLGVGALYWTWTAYQPAPRRLFPRQPMDFRSPA
                     ALDAAVQTAEKTLAENPENVDALLDIAIAQYERGPAQYKEALKNLEKAREAGAL"
BASE COUNT          403 a          689 c          642 g          356 t
ORIGIN      
        1 ccccgggccc cctattcccc ttcaggagag ctccagtgac gagcgaggtg cccgtgaaag
       61 acgatatgag catggaggaa ttgctggcgg aggcggaagc ccaaagccgg cccggttccc
      121 tggtcaccgc ccacgtggtg gaagtcaccc ccgcgggggt gctggtggat gtcggtttga
      181 aagttgaagg tctgatcccc ctcgcggagt ttcgttccca ggcccagccg ccgaaagtgg
      241 gcgattcctt ccccgcgttg atcaagcgcc tttccggccc cgagggccat cccctggttt
      301 ccttcaagga agcccgcgac cgttcccagt ggacccacgt gatccaggcc cgcgacgcgg
      361 gcacgcccct ggaagccacc gtcatccgtg ccgtcaaggg cggtttgatc gtcgacgtcg
      421 gcatggaggc cttcctcccc gcctcccagg tggaccgccg ccccgtcaag gatttgactc
      481 ccctggtcgg ccaaaaattt cccgtggccg tcttggaaat ggacacggcc aaagggaacg
      541 tcgtggtttc ccgccgcaag ctgttggaaa aagacgcctc ccaaaagcgc gccgacacct
      601 tgaagaccat ggaagtgggc aaagtgttca aaggcacggt caccagcctc acggccttcg
      661 gcgcttttgt ggacatcggc ggcatcgagg gcctgctccg catcacggac gtttcctggg
      721 ggtgggtcgg taaattgtcc gacgtcctca aggcgggcga cgtcgtcgac gtcaaagttc
      781 tgaaatttga tcccgccagt ggcaagatcg ccctgggtcg caaacagctc ctggccaaac
      841 cctgggattc cgtggacgcg aagttcccgg tcaattccat tcagaagggc aaagtgacca
      901 gcctgaccga ttttggggcc ttcgtggaat tggcccccgg cgtggaggga ttggttcacc
      961 agtcggagtt ctcctggaag gaacggggcg tgaagcccaa ggaacttttg accgtgggcc
     1021 aggaagtcta cgttttcatc ctgtccatca acaaagaaca ggaaaagatg gccttgagcc
     1081 tcaaacgcgc gggggaaaac ccctgggtgg aagccgcccg ggaatacccg gccggttccc
     1141 gcgtgaaggg cgtggtgacg cacatgttgc cggtgggcgc cttcgttcgc ctgccgagcg
     1201 gcatcgaagg ccttctccgc acccaggaca tgtcctggac caaggccccc gcgcacccca
     1261 aagaccttct caccgtcggc caggaaatcg aggcggtcgt gatcgatgta aatccgacca
     1321 cggagaagat ggccctgggt ctcaagcagt tgaccgagga ccccatcgcc aaattcgccc
     1381 ccggggccac ggtggaaggc aaagtggtcc gattgacgga ccaaggcgcc tttttggagc
     1441 tggcccccga catcgagggg ttcgtccaca tcagcgaaat tccatccgac catcggctca
     1501 accacgccgg cgaggtgctg aaggaaggcg agacggtgac cggcttggtg ctcaagggcc
     1561 agcgcaaatc gcgccgcatc gaattgtcca tccgcaagca cgatcaacgc gaagaacggc
     1621 agttgctcaa gcgttatcgc ccctccggcg aaggcgtcac cctgggcgac gtgaccgaat
     1681 ggggttccgc ggacgaatcc gccgccggcg agccccccgc ctccgattcc ccgaccccga
     1741 acgcctgagg tcgcggcgcc cgtgtcgcga ccggtcccgg cgacaacccg ttccgggatg
     1801 tccttcgacc ccctgattcc ggcattcctt ctgctcgggg tgggggccct ttactggacc
     1861 tggacggcgt accaacccgc gccccggcgc ctgttccccc ggcagccgat ggattttcga
     1921 tcccccgccg ccctggacgc cgccgttcaa acggccgaaa agaccctggc cgaaaacccc
     1981 gagaacgtgg acgccctgct ggacatcgcc atcgcccagt acgagcgcgg tcccgcccaa
     2041 tacaaggagg ccctcaagaa tttggaaaaa gcccgggaag cgggcgctct
//
LOCUS       DAOQLS010000376         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35665_length_2089_cov_4.435103, whole genome shotgun sequence.
ACCESSION   DAOQLS010000376 DAOQLS010000000
VERSION     DAOQLS010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35665_length_2089_cov_4.435103"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2089)
                     /locus_tag="PK362_07360"
     CDS             complement(<1..>2089)
                     /locus_tag="PK362_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74683.1"
                     /translation="LCLALGARAVMVNPDAERYFAVLSGTFQSGQYESAVAGCDTFLK
                     QFPRDPKAAAVKYLKAESFFRQSRFNEAAGEFKEFIGAYGSSQENLAISARLRLGECH
                     YNLKKFLTALDHFAWVEKSKNATLRAESLLGSAHCFLVRGEHGKAEIYLLKLLQSNAG
                     YANLPQVVVPLALIRMERGEYQDALSLLERAPDDPACQYYRGVCQRLSHRVIAAAQIL
                     KDLVDNDTAKVWTDKALFQMGEAYFQSKEYPLAYSAFKRIHEKELQSPLRPYALFRMG
                     CVNFQNGSFEAAGQNWTQLMKEFPENISGPSSQYLLAEIALRQNELGKAITGFGALAA
                     LDDYAMDAQYKVIWCLAVQGQYDQAIARSQKFLKDFEWGELYAKVSLLMGLCQQMMKQ
                     TDDAMTTYQSILDRYPNTAYYEKALYLLAVALVQDKRYAEVVTHVYTILKTAPASPSR
                     WQAETYYWVAESYFNIGQFELARQTYDLLIKNFRQTDLLPGATLGVAASLARLGLYDQ
                     AVETQARALELSQQMDNPEVKKTALLDSADVFFNKHEYEKAASFYEEFFNKYPDDSRA
                     DRALHQSGLALYRLEYFTDAIKKWTTLTERYPTSRLAPDAMFQSARTQFGLGQYPQAY
                     GYFRQIVEKYPESPLAKESMLMMGQCHYNAGDMPRAIEQYKAYMEKYPLDEKMGEVQD
                     LLQMAYYKQGKTGD"
BASE COUNT          399 a          579 c          653 g          458 t
ORIGIN      
        1 gtcgccggtt ttcccctgtt tgtagtaggc catttgcaac aaatcctgaa cctcccccat
       61 tttctcgtcc agggggtatt tttccatgta ggccttgtat tgttcgatgg cccggggcat
      121 gtcgccggcg ttgtagtggc actgccccat catcagcatg gattccttgg ccaggggcga
      181 ctcgggatat ttttcgacga tctggcgaaa gtaaccgtag gcctgggggt actggccgag
      241 gccgaactgg gtgcgggccg attggaacat ggcgtccggg gccaggcggc tggtggggta
      301 gcgttccgtc agcgtcgtcc actttttgat cgcgtcggtg aaatattcca ggcggtagag
      361 ggccaatccc gattggtgca gggcgcggtc ggcccgggaa tcgtccggat acttgttgaa
      421 aaattcctcg taaaagctcg cggccttttc gtactcgtgc ttgttgaaaa acacgtcggc
      481 ggaatccagc agggcggttt ttttgacctc gggattgtcc atctgctggg acaattccag
      541 ggcccgggct tgagtttcca cggcctggtc gtagagcccc aggcgggcca agctggccgc
      601 cacgcccagg gtggcgcccg gcagcaagtc cgtctggcgg aagtttttga tcaacagatc
      661 gtaggtttgc cgggccaatt cgaattggcc gatgttgaag taggattccg ccacccaata
      721 ataggtttcg gcctgccacc gggaggggct ggcgggggcg gttttcaaaa tggtgtagac
      781 gtgggtgacc acttcggcat accgcttgtc ctgcaccaga gccaccgcca gaagatagag
      841 ggctttttcg tagtaagccg tgttggggta tcggtccaaa atggattgat aggtcgtcat
      901 ggcgtcgtcg gtctgcttca tcatctgctg gcaaagaccc atcagcaacg acaccttggc
      961 gtagagctcg ccccactcga aatccttcag aaacttctgg gaccgggcga tggcttgatc
     1021 gtattgcccc tggacggcca agcaccagat gactttgtac tgggcgtcca tggcgtaatc
     1081 gtccagggcc gccagggccc caaaaccggt gatggccttg cccaactcgt tttgacggag
     1141 ggcgatttcc gccagaaggt actgacttga aggccccgaa atgttttcgg ggaattcctt
     1201 catcaactgg gtccaatttt gcccggcggc ttcgaaactg ccgttctgga agttgacgca
     1261 ccccatgcgg aacagggcgt agggccggag ggggctctgc agctccttct cgtgaatccg
     1321 cttaaaggcg ctgtaggcca gggggtattc cttggattga aaataggcct cgcccatttg
     1381 aaacagggcc ttgtcggtcc acaccttggc ggtgtcgttg tccaccaaat ctttgaggat
     1441 ctgcgccgcg gcgatcaccc gatgggacaa ccgctgacac acgccccggt aatactggca
     1501 agccggatcg tccggggccc gctccaggag cgacagggcg tcctgatact cgccccgttc
     1561 catgcggatc agggccagcg gcaccacgac ctggggcaaa ttggcgtatc cggcgttgga
     1621 ctggaggagt ttgagcaaat agatctccgc cttgccgtgt tcgccccgga ccaggaaaca
     1681 atgggcggac ccgagcaggg attccgcgcg aagggtcgcg tttttggatt tctccaccca
     1741 ggcgaaatga tccagcgcgg tcagaaattt tttaaggttg tagtggcatt cccccagccg
     1801 aaggcgggcg ctgatggcga ggttttcctg agaggacccg taggcgccga tgaattcctt
     1861 aaattccccc gcggcttcgt tgaagcggct ctggcggaaa aaggattcgg ccttcaggta
     1921 tttgacggcg gccgctttgg ggtcccgagg aaactgtttt aaaaaggtgt cgcagccggc
     1981 cacagcggat tcgtattggc cggactggaa cgtgccggac aacaccgcga aataccgctc
     2041 cgcgtcggga ttgaccatca ccgcccgggc gcccagcgcc agacagaga
//
LOCUS       DAOQLS010000377         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_35941_length_2081_cov_4.512340, whole genome shotgun sequence.
ACCESSION   DAOQLS010000377 DAOQLS010000000
VERSION     DAOQLS010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35941_length_2081_cov_4.512340"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..530
                     /locus_tag="PK362_07365"
     CDS             126..530
                     /locus_tag="PK362_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC74684.1"
                     /translation="MAVLAAVVWGLSGFVEKIGLRGADPQTGVLARSLGVGVAGVVLL
                     MVRPEVGTGFLALDWRTRLTLMGGGALASVIAQLFFYRALKVGDVGRVAAVGGAWPVV
                     AFVLSVLFLGETLTVRKGLGIFLVVLGAAFLR"
     gene            579..1076
                     /locus_tag="PK362_07370"
     CDS             579..1076
                     /locus_tag="PK362_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNC74685.1"
                     /translation="MAQEFSFDVVSEVDLQNADNAVNTANRELQTRFDFKGSVSRIDL
                     DKKDNKITILSDNEGKLKSVIDILQNRLIKQGISLKALDFQKIEPAEGGTVRQVAKVA
                     QGIPSDKAKDMVRALKDAKLKVNPSIQGEQLRVTGKSKDDLQAAMALLRGGDFGPPVQ
                     FKNFR"
     gene            1080..>2081
                     /locus_tag="PK362_07375"
     CDS             1080..>2081
                     /locus_tag="PK362_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74686.1"
                     /translation="MPPSPARRGLAVVAVALLVGGSVALIYFLQVLLVDDRKVSFPSE
                     DDRAAAPRFQSDPLIGRPGTGSFRAAGRAPEEIFHGLRALLTEHPAARLAWDAEARLP
                     AARVTLSDGSALVRRRALVSPPGGEYEFPVLVPADGWLEFQLGLLSMGAGLTCFVDVV
                     DERGAATPVFHDALVPRRHRAAHRLERWLPGFLSRALAQSTHHDERWRPVRVNLSAWA
                     RRSVRLRFRAEPGTTEGGEAGESAAFWGDPRVWGPRTEGTEPRRAGRIPTAGASVALV
                     VLEHARGDGISLEGFSEQTPPRLRDFFLEGVGVRRFYTNAVEPAGAWCALLTAETPRV
                     "
BASE COUNT          296 a          673 c          722 g          390 t
ORIGIN      
        1 cggctcgttc atggtttccc cattttttca cattttccgc gacatcgcca agagcgctcc
       61 cttatttcct aaaatacacg cgtgatcctt gtccgcgggg gaaccgccgc ctgaacgctt
      121 ttggaatggc tgttttggcc gccgtcgttt ggggcctgtc gggttttgtc gagaagatcg
      181 gattgcgggg cgccgatccc cagaccggcg ttttggcccg gtccctgggc gtcggcgtgg
      241 ccggcgtcgt tctgctcatg gtccgtcccg aggtggggac ggggtttctc gccctggatt
      301 ggcggacccg cctgacgttg atgggcgggg gcgctctggc cagcgtcatc gcccagttgt
      361 ttttttatcg ggccctgaag gtcggggacg tcgggcgggt ggccgcggtc gggggcgcgt
      421 ggcccgtcgt ggcctttgtt ttaagcgtgc tgtttctcgg cgaaaccttg accgtgcgaa
      481 agggcctcgg gatttttctc gtggtgctgg gcgcggcgtt tttgcggtga ccgcgaacgc
      541 gggcccccgg cgacgggcgg ccgcgcgggg gagggcgtgt ggctcaggaa ttctcgtttg
      601 atgtcgtcag cgaagtggat ctgcagaacg ccgacaacgc cgtcaacacg gccaatcggg
      661 agctgcagac ccggttcgat ttcaagggca gcgtgagccg catcgatctg gacaaaaaag
      721 acaacaagat cacgatcctt tccgacaacg aaggcaagct caaaagcgtg atcgacattt
      781 tgcagaaccg cctgatcaaa caaggcatct ccctgaaggc cctggatttc cagaagatcg
      841 aacccgccga gggcggaacg gtccgccagg tggccaaagt cgcgcagggc atcccgtccg
      901 acaaggccaa ggacatggtg cgggccctga aagacgccaa attgaaagtg aacccctcca
      961 tccagggcga acaactccgg gtcaccggca aatccaagga cgatctgcag gccgccatgg
     1021 ccctcctgcg gggcggcgat ttcggcccgc ccgtccaatt caagaacttc cgctaacgca
     1081 tgcccccctc ccccgctcga cggggactcg ccgtcgtcgc cgtcgccctg ctggtcgggg
     1141 ggtcggtggc gttgatttac ttccttcagg tgctgttggt ggacgaccga aaagtctcct
     1201 tcccgtccga agacgatcgc gccgcggcgc cccgctttca atccgacccg ttgatcggcc
     1261 ggccggggac cgggtccttt cgcgccgccg gccgcgcccc cgaggagatc ttccacggcc
     1321 tgcgggcgct gttgaccgag caccccgccg cgcgtttggc ctgggacgcg gaggcccgcc
     1381 ttccggccgc gcgggtcacc ctgtccgacg gctcggccct cgttcgtcgg cgggccctgg
     1441 tgtcgccgcc gggcggcgaa tacgagttcc cggtcttggt ccccgcggac ggctggctgg
     1501 aatttcaact gggacttctc tccatgggcg cggggctgac ctgtttcgtc gacgtggtgg
     1561 acgagcgggg cgcggcgacg cccgttttcc acgacgctct cgttccccgg cgccaccggg
     1621 ccgcccatcg cttggagcgg tggctcccgg gctttctttc ccgggccctg gcccagagca
     1681 cccaccacga tgaacggtgg cggccggtcc gcgttaattt gtcggcctgg gcccggcgat
     1741 ccgtccgcct tcggttccgc gcggaaccgg gaacgacgga gggcggcgag gccggcgaat
     1801 cggcggcttt ttggggggac ccccgggtgt gggggccccg gaccgagggg accgagcccc
     1861 ggcgggcggg acgaatcccg accgcggggg cctcggtggc cctggtcgtt ctggaacacg
     1921 cccgggggga cggcatcagt ttggagggat tttcggagca aacgccgccg cgcctgcggg
     1981 actttttcct ggaaggggtc ggggtccgcc ggttttacac caacgcggtg gagcccgcgg
     2041 gggcctggtg cgcgctgctg acggccgaaa cgccccgggt c
//
LOCUS       DAOQLS010000378         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36077_length_2077_cov_4.075668, whole genome shotgun sequence.
ACCESSION   DAOQLS010000378 DAOQLS010000000
VERSION     DAOQLS010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36077_length_2077_cov_4.075668"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK362_07380"
     CDS             complement(<1..350)
                     /locus_tag="PK362_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74687.1"
                     /translation="MTASSLDLNDHVFGKPREVLCLGIALALHVPLLFWKGHPDAGPL
                     GDPIVGVDFVVEEEAKPEPEPPKKEEPKEASFFERVQKMVGLATKPAPVEKLLQEAPK
                     PELAGTPSAGPIQV"
     gene            complement(347..766)
                     /locus_tag="PK362_07385"
     CDS             complement(347..766)
                     /locus_tag="PK362_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC74688.1"
                     /translation="MKKVRDQGAGISGINITPIIDITLVLLIVMLVAAPVLNIPNMEV
                     TLPDAYTAETKEKNISVSLGTDLRVAIDEDIVAVGDLPRRLDDKLKKKNNPLVIIRAD
                     KDVDYEDVENLMEVIKTKTRAKKIAIATRQKEVRAAP"
     gene            complement(763..1191)
                     /locus_tag="PK362_07390"
     CDS             complement(763..1191)
                     /locus_tag="PK362_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC74689.1"
                     /translation="MKKGSIEKGEAEEISEINVVPLADVSLVLLIVLMLITPMVMQSM
                     IQVYASRGAAAVKADQTVKEKPLFLDVRTDGYYLNTNKLTTELQLLTNLRGELSRKDD
                     RTVIITAGKGISHGRMVTALDTAKQAGAEKLSLVKRAAKS"
     gene            complement(1203..1817)
                     /locus_tag="PK362_07395"
     CDS             complement(1203..1817)
                     /locus_tag="PK362_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC74690.1"
                     /translation="MGFKEWFMINPPIISTILVFSVLLLAFTAERFWVFRKEAKFPRE
                     LWERIMGLVREKRLRDAIALCEVSHGAFARVFKAGLEGSLVSRLDAEDGMVIEKEEGQ
                     EFLRKRVGLFGTISFISPLIGLLGTVLGVLRAFHDLARSGSGGPSVVAAGISEALITT
                     VAGLVVAVPAAIIYNYFNFRLRATLVEMNTYGQRLLLAVFGDKR"
     gene            complement(1828..>2077)
                     /locus_tag="PK362_07400"
     CDS             complement(1828..>2077)
                     /locus_tag="PK362_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74691.1"
                     /translation="GRCFKKLRAPAEEKAAYRSLQALTPRDNKFRLAGLVMLGEALEG
                     DGAKADAVAVYQDIASHSTNAEWRAIAQEKISALKGGK"
BASE COUNT          373 a          636 c          630 g          438 t
ORIGIN      
        1 ctcacctgga tcggaccggc ggagggggtt ccggccagtt cgggtttggg ggcttcttgg
       61 agcagttttt cgacgggcgc gggcttggtg gccagcccca ccatcttttg gacgcgttca
      121 aaaaaactgg cttctttggg ctcctctttc ttggggggtt ccggctcggg cttggcttcc
      181 tcctcgacca cgaaatccac gcccacgatg gggtcgccga gaggcccggc gtccgggtgt
      241 cccttccaaa acaaaagggg cacgtgcagg gccagggcga tcccgaggca cagcacctcc
      301 cggggtttcc cgaaaacgtg atcgttcaga tccaacgaac tggcggtcat ggcgcggcgc
      361 ggacttcctt ttgccgggtc gcgatggcga tctttttggc ccgggttttc gttttgatga
      421 cttccatcaa attctccacg tcctcgtaat ccacgtcctt gtcggcccga atgatgacca
      481 ggggattgtt tttctttttc agcttgtcgt ccaagcgacg gggaaggtcg cccacggcca
      541 cgatgtcttc gtcgatggcc acccgcaggt ccgtgcccaa cgacaccgaa atattctttt
      601 ccttggtttc ggcggtgtac gcgtcgggca gggtgacttc catgttcgga atgttgagga
      661 cgggggccgc cacaagcatg acgatgagca acaccagagt gatgtcgatg atgggcgtga
      721 tgttgatgcc cgaaatgccg gcgccctggt cccggacctt tttcacgatt tggccgcccg
      781 tttgacgagg gagagctttt ccgccccggc ctgcttggcc gtgtcgaggg ccgtcaccat
      841 ccgcccgtgg gagatgccct tgcccgccgt gataatgacc gtgcggtcgt ccttgcggct
      901 caactcgccc cgcaagttcg tgagaagctg gagttccgtg gtcagcttgt tggtgtttag
      961 gtaatacccg tccgtgcgga cgtccaggaa gaggggcttt tccttcacgg tctgatccgc
     1021 cttcacggcg gcggcgcccc gggaggcgta gacctggatc atggactgca tgaccatcgg
     1081 ggtgatcagc atcaaaacga tgaggaggac cagcgaaacg tcggccagcg gcacgacgtt
     1141 gatttcgctg atttcttccg cctcgccttt ttcgatggac cctttcttca ccgggaatcc
     1201 ccctagcgct tgtccccgaa gacggcgagc aacagccgtt ggccgtaggt gttcatttcc
     1261 accagcgtcg cccgcaggcg gaagttgaaa tagttgtaga tgatggccgc gggaacggcg
     1321 accacaaggc ccgcgacggt cgtgatcaaa gcttccgaaa taccggccgc caccaccgac
     1381 ggccccccgg aacccgatcg cgccaggtcg tgaaaggcgc gcaacacgcc gagcacggtg
     1441 cccaacagcc cgatcaaggg cgaaataaag ctgatggtgc cgaagaggcc gacccgcttg
     1501 cgcaggaatt cctggccttc ctctttttca atgaccatgc cgtcttcggc gtccagccgg
     1561 gaaacgaggg agccttccag accggctttg aaaacccggg cgaaggcgcc gtgggaaacc
     1621 tcgcacaagg cgatggcgtc ccgaagcctt ttttcccgga ccaggcccat gatgcgttcc
     1681 cagagttccc ggggaaattt ggcctctttg cggaacaccc agaaccgctc ggcggtgaac
     1741 gccaacagca gcaccgaaaa caccaaaatc gtgctgatga tgggcgggtt gatcatgaac
     1801 cattctttga agcccatgga tgtcatctta cttgcctccc ttcagtgcgg aaatcttttc
     1861 ctgcgcgatg gcccgccact ccgcgttggt gctgtgggag gcgatgtcct gataaacagc
     1921 gacggcgtcc gctttggccc cgtcgccctc caacgcctcg cccaacatca ccaacccggc
     1981 gaggcggaat ttgttgtccc ggggcgtgag ggcctgcagg gaccggtagg ccgctttttc
     2041 ctcggcgggg gccctcagtt tcttgaagca acgaccc
//
LOCUS       DAOQLS010000379         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36142_length_2075_cov_4.047030, whole genome shotgun sequence.
ACCESSION   DAOQLS010000379 DAOQLS010000000
VERSION     DAOQLS010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36142_length_2075_cov_4.047030"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PK362_07405"
     CDS             <1..1082
                     /locus_tag="PK362_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNC74692.1"
                     /translation="SLSWGMVVLVLGILAWFLHSVRLILPPFVMAAILAYLLNPVVSF
                     FEIRGLRRDTVVLILFALLVAAAVGLAYGGLVAIWQDLPDLRAQWPAYMRQADSAVVR
                     LQNDLSEYVWVLKERRLLEKGVNAAMAWVEQSLWHSPSVVASALTLSVNVLLTPFIAF
                     FFLRGGAKLAQGVLDICPGRWVERFLSLFNKFDEVLGNYTRGVVFEAFLVGLFSVAGL
                     TAIGLDYAALIGIATGIGNMIPYLGPLLGGAVGLVVALFQFGTLPALAKVLIIFTVVH
                     YIDSYVLQPLIMKRSVNLTPVTVVFALLCGGHLAGLWGLVFAVPVASLIKEASRIFFD
                     WYRAERGYLAPAKDIALAAARPWVV"
     gene            1386..1461
                     /locus_tag="PK362_07410"
     tRNA            1386..1461
                     /locus_tag="PK362_07410"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1419..1421,aa:His,seq:gtg)
     gene            1562..1942
                     /locus_tag="PK362_07415"
     CDS             1562..1942
                     /locus_tag="PK362_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74693.1"
                     /translation="MRCCAALLLMLGLFGTVPFQRFVPRPAASCEICGHACCCPTVCA
                     KRRAARAAAQCEAPESLCRIQPAAAGLPGWWAERLERPGMLPRHEDPAIDPISSLRCG
                     GEKQLLFLSSPDRGVPLPPPRSRT"
BASE COUNT          296 a          659 c          621 g          499 t
ORIGIN      
        1 tttccctctc ctgggggatg gtggtattgg tcctcgggat tctcgcgtgg ttcctccatt
       61 ccgttcgcct catccttccg cccttcgtga tggcggcgat tctggcctac ctgctcaacc
      121 cggtggtttc cttttttgaa atacgcggcc tgcgacggga cacggtggtt ttgatcctct
      181 tcgcgctcct ggtggccgcg gccgtcgggc tggcctacgg gggcctcgtg gccatctggc
      241 aggatctgcc cgatcttcgg gcccaatggc ccgcgtacat gcgccaggcc gattcggcgg
      301 tggtgcgcct tcaaaacgat ttgtccgaat acgtctgggt gctcaaggag cgccgtctct
      361 tggagaaagg ggtcaacgcg gccatggcct gggtggagca gagcctttgg cattccccgt
      421 ccgtcgtcgc gtcggccctg accctctcgg tcaacgtgct cctcacgccc ttcatcgcgt
      481 ttttcttttt gcgcggtggg gccaagctgg cccagggggt gttggacatc tgccccgggc
      541 gctgggtgga acggtttctg agtttgttca ataagttcga cgaggtgttg ggcaactaca
      601 cccggggggt cgttttcgag gcgtttttgg tggggctctt ttccgtcgcc gggctcaccg
      661 ccatcgggtt ggactacgcc gccctgatcg gcatcgccac gggcatcggc aacatgattc
      721 cttacctggg gccgctcctg gggggagccg tcggcctggt ggtggccctt tttcagttcg
      781 gcaccttgcc ggcgctggcc aaggttctca ttatctttac cgtggtgcat tacatcgaca
      841 gctacgtcct ccagccgttg atcatgaaac ggagcgtcaa tctgacgccc gtgaccgtgg
      901 ttttcgccct tctgtgcggg gggcacctgg ccgggctctg ggggttggtc ttcgcggtgc
      961 ccgtggcgtc gctgatcaag gaagccagcc gcatcttttt tgattggtac cgcgccgagc
     1021 gcggttacct ggcccccgcc aaggacatcg ccctggccgc cgcccgcccc tgggtggttt
     1081 aaccgtcgga aaatcgacgt cgcccctccc gcagtcggcc aaaatcatct aaaatcaacc
     1141 ccgttcgtcc ccaacacccg atttacggtg ggtatagctc tggcggggtt tcgggtcctc
     1201 tggacccgcc gccggtcctt tcgctccgcg aaagggcagc gtccgaggaa atggacgtga
     1261 aacgaaacaa tttacggtgg gtatagctct ggcggggttt cgggtcctct ggacccgccg
     1321 ccggtccttt cgctccgcga aagggcagcg tccgaggaaa tggacgtgaa acgaaacaat
     1381 ttacggtggg tatagctcag cggttagagc gtccggctgt ggcccggaag gtcgcgggtt
     1441 caaatcccgt tactcacccc aatttgtaga agtatgggct gtctattttc cttccttcgg
     1501 cgacaaaccg ttgccgccaa ttgtccttga attcctccaa acgagaacat aatatcccgt
     1561 catgcgctgt tgtgccgcac ttctgttgat gctcgggttg ttcgggaccg tgccgtttca
     1621 gcggtttgtc ccgcgccccg ccgcctcctg cgaaatatgc ggccacgcct gctgctgccc
     1681 caccgtttgc gccaaaaggc gcgcggcccg ggccgccgcc caatgcgaag cccccgagtc
     1741 cctttgccga attcaacccg cggcggcggg acttcccggt tggtgggccg aacgcttgga
     1801 acggcccggc atgctccccc gtcacgagga tcccgcgatc gacccgattt cttcgcttcg
     1861 ttgcggaggg gagaaacagc ttctctttct atcttccccg gaccgcggcg ttcctcttcc
     1921 tcccccccgt tcccggacct gattttcatc ccccttcgcg gccgcttgcg gtcgcttatc
     1981 ccgtcggacc cgatccaggc caggaccggg gccgctttct atccatttca aggagtttct
     2041 tatgcgtcgt ttatggtttt tgattaaccg attgg
//
LOCUS       DAOQLS010000380         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36217_length_2073_cov_3.583251, whole genome shotgun sequence.
ACCESSION   DAOQLS010000380 DAOQLS010000000
VERSION     DAOQLS010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36217_length_2073_cov_3.583251"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK362_07420"
     CDS             <1..196
                     /locus_tag="PK362_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNC74694.1"
                     /translation="NTALREAFAEHHKKHRVHTAAPALCTDNGVMIGVAGYYKYLRSA
                     KKRFSPDALSVDSTLALKNW"
     gene            213..1250
                     /gene="dusB"
                     /locus_tag="PK362_07425"
     CDS             213..1250
                     /gene="dusB"
                     /locus_tag="PK362_07425"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNC74695.1"
                     /translation="MTSSATLPRPVLGTLRLDNPFVLAPMAGIGDPPYRRLCRRGGAG
                     LVCAEMVSANALHFGDEKSRRMLRTYPDEHPVSLQVFGSDPDRLADAARAAEAAGADV
                     VDLNCGCPVPKIANNGAGISLLKDESLFARCLAAMVKAVKIPVTFKTRLGFRRGENIA
                     RRFVQLAESVGVSAVAIHARSREDYHVGPPNNAALREIVESVRIPVFGNGGVRNFADA
                     KRMMDESGCVGVLIGQAAVGNPYLFSELAHAARAGVEAPLPVAQRLALFREHARLIAE
                     FYGEDLGVRRFRKYIPSYVHGLRGSAAFRSRANVTNSLASLLRLIDEFDAALRDFASP
                     TVDIDTPWTVC"
     gene            1298..>2073
                     /locus_tag="PK362_07430"
     CDS             1298..>2073
                     /locus_tag="PK362_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNC74696.1"
                     /translation="MSIKFGTSGWRAIMADEFTFANVRIAVQSIADYLREERLHNKPI
                     VIGYDTRFLSEDFAKTAAGVLAANGMESLLCGRDTPTPVVAFEVLRNKAAGGIIITAS
                     HNPATYSGLKFNSAWGGPALPQITQKIEAGAEPYLAGEAIPKTSREAVGLKKRLIVPH
                     DPMPAYFKQLDELVDRSLLKRGKIRVVTDALWGAGRGYLDEYLRKAGVEVNALHTNRD
                     VLYNGHGPSPDPETLTELQATVKKSKANLGLATDGDADRFG"
BASE COUNT          372 a          715 c          618 g          368 t
ORIGIN      
        1 caacacggct ttgcgggaag ccttcgcgga acaccacaaa aagcaccggg tgcacaccgc
       61 cgcgcccgcc ctttgcaccg acaacggcgt catgatcggc gtcgcggggt attacaagta
      121 ccttcgctcc gccaagaaac ggttttcgcc cgacgccctg tccgtggatt ccaccctcgc
      181 cctcaagaac tggtaaccgc cgtttttcgc ccatgacgtc ctccgcgacc cttccccgtc
      241 ccgtcctcgg gaccctgcgg ctggacaatc ccttcgtcct ggcccccatg gccgggatcg
      301 gcgacccgcc gtaccgccgt ttgtgccgtc ggggcggcgc cggattggtg tgcgccgaaa
      361 tggtctcggc caacgccctc cacttcggcg acgagaaatc ccggcgcatg ctccgcacct
      421 atcccgacga acacccggtg agccttcaag tgttcggcag cgatccggac cgattggccg
      481 acgcggcccg ggcggccgag gcggcgggcg ccgacgtggt ggatttgaac tgcggctgcc
      541 ccgtgcccaa aatcgcgaac aacggcgccg gcatcagcct gctcaaggac gagtcgctct
      601 tcgcgcggtg tctggcggcc atggtgaagg ccgttaaaat ccccgtgacc tttaaaacgc
      661 gtctgggatt tcgtcggggg gaaaacatcg cccggcggtt tgtccagctg gccgaatcgg
      721 tgggcgtctc ggcggtggcc attcacgccc gttcccggga ggattaccac gtgggcccgc
      781 ccaacaacgc ggcccttcgg gaaatcgtcg aatccgtacg gatccccgtg ttcggcaacg
      841 gcggtgtgag aaatttcgcg gacgccaaac gcatgatgga cgaatccggc tgcgtggggg
      901 tgttgatcgg tcaggcggcg gtcgggaacc cctacctttt ttcggagctc gcccacgccg
      961 cccgcgccgg cgtcgaggcg cccctgccgg tcgcccagcg attggccctt ttccgggaac
     1021 acgcgcgact gatcgcggag ttctacgggg aggatctggg cgtccggcgt ttccgcaaat
     1081 acattccctc ctacgtccac gggcttcggg ggtccgcggc gttccgctcc cgggccaacg
     1141 tcacgaattc cctggcgtcc ctccttcgat tgatcgatga atttgacgcc gctctgcggg
     1201 atttcgcctc tcccaccgtc gacattgaca cgccatggac cgtgtgctaa catgggccgg
     1261 attctaaaac gtctctaaaa ataagggttt ttattcaatg agcatcaagt ttggcacctc
     1321 gggttggcgc gccatcatgg cggacgaatt caccttcgcc aacgtccgca tcgccgttca
     1381 gtccatcgcc gattatcttc gcgaagagcg cctccacaac aagccgatcg tcatcggcta
     1441 cgacacccga ttcctctcgg aagatttcgc caaaacggcg gccggcgtcc tcgccgccaa
     1501 cggcatggag tcgctgctct gcggccgcga cacccccacc cccgtggtgg ccttcgaggt
     1561 gctccgcaac aaggccgcgg gcggcatcat catcacggcg agccacaacc cggcgacgta
     1621 ttcgggcctc aaattcaatt ccgcctgggg cggtcccgcc ctcccccaaa tcacgcaaaa
     1681 aatcgaggcc ggcgcggagc cctatttggc cggcgaagcg atccccaaga ccagccgcga
     1741 agccgtgggt ctcaagaagc gcctgatcgt gccccacgat ccgatgcccg cctatttcaa
     1801 gcagttggac gagttggtgg accggtccct gttgaaacgc ggaaaaatcc gcgtcgtgac
     1861 cgacgcgctc tggggcgcgg gccgcggcta tctggacgaa tacctccgca aggccggggt
     1921 ggaagtgaac gccctccaca cgaaccggga cgttctctac aacggccacg gcccgtcccc
     1981 cgacccggaa acgctgacgg aactccaggc gacggtaaaa aaatccaagg cgaatctggg
     2041 gctcgccacc gatggggacg ccgaccgttt cgg
//
LOCUS       DAOQLS010000381         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36274_length_2071_cov_4.014881, whole genome shotgun sequence.
ACCESSION   DAOQLS010000381 DAOQLS010000000
VERSION     DAOQLS010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36274_length_2071_cov_4.014881"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1823
                     /locus_tag="PK362_07435"
     CDS             198..1823
                     /locus_tag="PK362_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNC74697.1"
                     /translation="MTPAPQGFLNLVLHAHLPYVRHPEYPDFLEEDWFYEAITETYVP
                     LLDMMERLAGENVHFRLTMSLTPPLCNMLADELLMSRYRVKLNALIELAEKEVLRTRQ
                     LPAAYQETANMYLSRFRRVREIVDASGGRLLQRFRALQDHGLLEIITCGATHGYLPLM
                     IHEESRRAQIRVAVRDYQRHFGRPPRGIWLPECAYAPGVDTLLKEEGITFSFLDSHGL
                     LFGKPRPRHGVFAPIFAPGGVAFFGRDMETAQQVWSAEQGYPGDLSYREFYRDVGYDL
                     DYNYIRPYLHEDGVRRNIGIKYHRVTGRVALADKEPYQPARARETAAAHAGNFLFNRE
                     RQIEYLKGVMNRPPVVTSIYDAELFGHWWFEGPDFLECLLRKIHFDQNLFKTTNGVEY
                     LAQVPEQQIVEPEPSSWGDKGYHEVWLNASNDWIYPHLHIAAERMIRLANIHPQADGL
                     LRRYLNQAARELLLAQSSDWAFLMTVGTARQYSEKRTRDHIGRFLDLFHRINENRMEE
                     GPLAPIEGADNIFPDIDYRAYQSAPQRETAGIR"
     gene            complement(1820..>2071)
                     /locus_tag="PK362_07440"
     CDS             complement(1820..>2071)
                     /locus_tag="PK362_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC74698.1"
                     /translation="RVIFEGSSLASVMRLAAAGEGVTLVPRVAAETGGLPLRFIPFER
                     PEPHRQVGALWRSGAALTLGQKAVVEALAGAWEAGEKRG"
BASE COUNT          404 a          739 c          563 g          365 t
ORIGIN      
        1 tccaaggccg cccgattccg ctcaaccccg acggcacgtt ctccttccgt ttcgccctgc
       61 cggacggaaa acaaaatttc ccgatccacg cgaccaacgc cgacggcgat ttgtcccgtt
      121 cgatcgaatt cgtcgtctcg cgcgaaacgc gcaaagggcg ggagtgacgc cgcgatgact
      181 tcccgggccc gcctttcatg acgccggcgc cccagggctt tttaaatctc gtcctgcacg
      241 cgcacctgcc ctacgtgcgc catcccgaat accccgactt cctcgaagag gattggttct
      301 acgaagccat cacggaaacc tacgtgccgc tcctggacat gatggagcgc ctggccggag
      361 aaaacgtcca cttccgcctg accatgagcc tcaccccccc gctttgcaac atgctcgccg
      421 atgaactgct catgtcgcgc taccgcgtga agctgaacgc gctcatcgaa ctggcggaaa
      481 aagaggtgtt gcgcacccgg caattgcccg ccgcctatca ggaaaccgcg aacatgtatc
      541 tctcccggtt tcgccgcgtg cgcgagatcg tggacgcctc cggcggccgt ttgctccaac
      601 ggttccgggc ccttcaagat cacggccttc tggaaatcat cacctgcggc gccacccacg
      661 gctatttgcc gctcatgatc cacgaagaat cccgccgggc ccagatccgc gtggccgtgc
      721 gggattacca acgccacttc ggccgaccgc cccggggcat ttggttgccc gagtgcgcct
      781 acgccccggg cgtcgacacc ctgctcaaag aagaaggcat caccttttcg tttttggatt
      841 cccacgggct cctgttcgga aaaccccgac cccgccacgg ggtgttcgcg ccgattttcg
      901 cgccgggcgg ggtggctttt ttcggccggg acatggaaac cgcccagcag gtttggtccg
      961 ccgagcaggg ttaccccggc gacctttcct accgggagtt ttaccgggac gtggggtacg
     1021 atctcgatta caactacatc cgcccttacc tgcacgaaga cggagttcgg cgcaacatcg
     1081 gcatcaaata ccaccgcgtc accggacggg tggccctggc cgacaaggaa ccttaccagc
     1141 cggcccgggc ccgggaaacc gccgccgccc acgcggggaa cttccttttc aaccgcgaac
     1201 gtcaaattga atatttaaaa ggcgtgatga accgcccgcc cgtggtgacc tccatttacg
     1261 acgcggaact cttcggccac tggtggttcg aggggccgga ctttttggaa tgcctgcttc
     1321 gaaaaatcca tttcgaccag aacctgttca agaccaccaa cggcgtggaa tatctggccc
     1381 aggtgccgga acagcagatc gtcgaacccg aaccctccag ctggggcgac aagggctacc
     1441 acgaagtctg gctgaacgcc tccaacgact ggatctaccc ccacctgcac atcgccgcgg
     1501 aacggatgat ccgcctggcc aacatccacc cccaagccga cggccttctc cgccgttact
     1561 tgaaccaggc ggcccgggaa ctcctgttgg cccaatcctc ggattgggcg tttctgatga
     1621 ccgtggggac ggcgcgccaa tattccgaaa aacggacccg ggaccacatc ggacgtttcc
     1681 tggatttgtt ccaccggatc aacgaaaacc ggatggagga aggccccctc gcccccatcg
     1741 aaggggccga taacattttc ccggacatcg attaccgggc ctaccaaagc gctccccagc
     1801 gggaaaccgc cgggattcgt taacccctct tttctcccgc ctcccaagct cccgccaggg
     1861 cctcgaccac ggctttttga cccaacgtga gggccgcccc cgatcgccac agggcgccga
     1921 cttgccgatg gggctccgga cgctcgaagg ggatgaaccg gagcggaagc ccgcccgttt
     1981 ccgccgccac gcggggcacc aacgtcacgc cctcccccgc cgccgccaac cgcatgaccg
     2041 acgccagact gctgccctcg aaaatgaccc g
//
LOCUS       DAOQLS010000382         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36277_length_2071_cov_3.789187, whole genome shotgun sequence.
ACCESSION   DAOQLS010000382 DAOQLS010000000
VERSION     DAOQLS010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36277_length_2071_cov_3.789187"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2071
                     /locus_tag="PK362_07445"
     CDS             <1..>2071
                     /locus_tag="PK362_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC74699.1"
                     /translation="DVADVKDFYLEAQSYARLNKKPTVSIYVQKENNSNTIRVAERVL
                     KVANRVRDNVLGSDIQLQVVTDQSVFIREAMENVTSNLTWGMLFTLLVIWYFLKEWRH
                     TILVFLSAPIAALITLAFMWITGLSLNVMTLSALALGIGIVVDSATVVLENILDKKRE
                     AAHHPEMNLEEDSIKATEELFISLVGGTLTTIVVFLPIVFINKQVKILYSGLAYTIAF
                     SMAASLLVAVTLIPLLASRISLPKHNGFIAPETHEKIRQFWADCPPFLHRAAAFLMRV
                     KDLIFPTAAPVLRGEAPPGRWARLKSFWFERLWPAVHREFLKLRAHPRRTYVHWCAWC
                     LRYQRVISVVMAVATAGAMMMYLFVLEKDFMGTTEQNEFIIFIELPAGAKLDISDQVV
                     REVEKVLSDTPEIAGVVKTAAARVEGWSSKVYVTLRPRAERSRSIQDIITDLRPKVAD
                     IGQQFDSFVYFSEPESSKEFLIDTFGPEYTNLRDMASGIAQKLQSVKGLTDIKLRYKP
                     GQPEVQIKVDKERAALFGLTIKDVSEDLHARIRGLRPTYFYTENAEIEIIARDQEKFR
                     KTLEDVHYLTLTGPDGTLIPIDQLAEFNFALTPSEVWRKDKQRVIQVSANRDKLPLST
                     AAKRTLSAMKGLEAPTGYYFQIGGDYVDMVQNEKEFRFAFFIMVGLVFIVLASLFESY
                     IQALMIMV"
BASE COUNT          450 a          616 c          549 g          456 t
ORIGIN      
        1 ggacgtggcc gacgtcaaag atttttatct cgaagcccaa agctacgccc gcctgaacaa
       61 aaagccgacc gtctccattt acgtccaaaa ggaaaacaat tccaacacca ttcgcgtggc
      121 ggaacgtgtc ttgaaagtcg ccaatcgggt ccgcgacaac gtgttgggtt ccgacatcca
      181 actgcaggtc gtgaccgatc aatccgtgtt tattcgggaa gccatggaaa acgtcaccag
      241 caacctcacc tggggcatgc tcttcacctt gctggtcatt tggtatttcc tcaaggaatg
      301 gcggcacacg attctcgtgt ttttgtccgc cccgatcgcg gcgctgatta ccctcgcctt
      361 catgtggatc acggggcttt ccttgaacgt gatgaccctc tcggccctgg ccctgggcat
      421 cggcattgtg gtcgacagcg cgacggtggt tttggaaaac atcttggaca aaaagaggga
      481 agccgcccat caccccgaaa tgaatctgga agaagactcc atcaaggcca ccgaagaact
      541 tttcatctcc ctggtcgggg gaacgctgac gaccattgtg gtgtttctgc cgatcgtctt
      601 catcaacaaa caggttaaaa ttctttattc cggcttggcc tacaccatcg ccttttccat
      661 ggccgcatcg ctgttggtgg ccgtgacgtt gatcccgttg ctggcctccc ggatttccct
      721 tccaaaacac aacggtttca tcgcgccgga aacccatgaa aaaatccgcc aattttgggc
      781 cgactgtccg cccttcctcc atcgagccgc ggcgtttctg atgcgggtga aggatttgat
      841 ttttccgacc gccgcgccgg tcctccgcgg ggaggcgccc ccgggtcgat gggcgcgttt
      901 aaaatccttc tggttcgaac ggctttggcc ggccgtccac cgggagttcc ttaagctgcg
      961 cgcccatcct cgccgaactt acgttcattg gtgcgcctgg tgcctccgtt atcaacgggt
     1021 catttccgtg gtcatggccg tcgccaccgc cggggccatg atgatgtacc tcttcgtctt
     1081 ggaaaaggat tttatgggga ccaccgaaca aaacgaattc atcattttca ttgaactccc
     1141 cgccggggcc aagctcgaca tttcggatca agtggtgcgg gaggttgaaa aagtcctttc
     1201 cgacacgccg gaaatcgccg gggtcgtcaa aacggccgcg gcccgggtgg agggttggtc
     1261 ctcgaaggtc tatgtcacgt tgcgcccccg ggcggaacgg tcccgatcca ttcaggacat
     1321 catcacggat ctccggccga aagtggcgga catcggccaa cagttcgaca gtttcgtcta
     1381 tttttcggaa cccgaaagtt cgaaggaatt tctgatcgac acctttgggc ccgaatacac
     1441 gaacctccgg gacatggcct ccggcatcgc ccaaaaactc caaagcgtca aaggcctgac
     1501 ggacatcaag ctgcgctaca agcccggcca accggaagtc caaatcaagg tggacaagga
     1561 gcgcgccgcc cttttcggac tgaccatcaa ggacgtttcc gaagacctcc acgcccgcat
     1621 ccggggcctt cgtccgacct atttttacac ggaaaacgcc gaaatcgaaa tcatcgcccg
     1681 ggaccaggaa aaattccgaa aaacgctgga ggacgtccac tatttgaccc tgacggggcc
     1741 cgacggaaca ttgattccca tcgaccaact cgcggaattc aactttgcct tgacccccag
     1801 cgaagtttgg cgcaaggaca aacagcgcgt gatccaggtg agcgccaacc gcgacaaatt
     1861 gccgctgagc accgcggcca agcggactct gtcggccatg aagggcctgg aggcgcccac
     1921 gggttactat ttccagatcg gcggcgatta cgtcgacatg gtccaaaacg agaaagagtt
     1981 ccgctttgcc ttttttatca tggtcggtct ggtgttcatc gtgttggcga gcctgtttga
     2041 atcgtacatc caggctttga tgatcatggt c
//
LOCUS       DAOQLS010000383         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36441_length_2066_cov_2.236201, whole genome shotgun sequence.
ACCESSION   DAOQLS010000383 DAOQLS010000000
VERSION     DAOQLS010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36441_length_2066_cov_2.236201"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PK362_07450"
     CDS             complement(<1..395)
                     /locus_tag="PK362_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74700.1"
                     /translation="MVAVTVTEIHAFGRDGKPLWSLPKRFYSPAVLAEGLLWYQHVSH
                     FLNSVDAEGSVVNKEESFPGALGETEQVELLYPRKDHFISSDYDLNQRDHDEMTGVNM
                     SSDPELEIRKNVFGSAYSDFQKRFERTSEA"
     gene            complement(697..1173)
                     /locus_tag="PK362_07455"
     CDS             complement(697..1173)
                     /locus_tag="PK362_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74701.1"
                     /translation="MKWLFLLILLTGGSIMEPENVSSSAPAKFNGPDSYREYTGVVTS
                     AKTVKETIGTSDATAEERAFLEILLTVEEARTIEIPEFDPTVPLEKEAVPGTKPAVKE
                     NPELKGKTVRFYFPPKEAIAVGDRLDITTRARSAISRGWISGEHGATYKKVNKGPK"
     gene            complement(1245..1799)
                     /locus_tag="PK362_07460"
     CDS             complement(1245..1799)
                     /locus_tag="PK362_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74702.1"
                     /translation="MYYFVRQNLFETLNIIDFNPPASEVTISDWVNVDATVNPAAPLV
                     CELLPEYGSEFADYFDVTLPVMSRRLIAFLKQLGVDNFQQYPVVFKHPTTGREYRDHS
                     AVKFSGVYDAIDRERSEKKIDFGEVCFEGSIVIDPKRVGELSVFRLKDGPNFLVATER
                     VANALKTQNFKALLLQPTEEYSGT"
BASE COUNT          421 a          652 c          553 g          440 t
ORIGIN      
        1 gcctcggacg tgcgttcaaa ccttttttga aaatcggaat aggcggaccc gaatacattc
       61 tttcgaattt ccaattccgg gtcggaggac atattgaccc cggtcatttc gtcgtggtct
      121 ctctggttca aatcgtaatc cgacgaaata aaatgatctt ttcgggggta caaaagctcc
      181 acctgctcgg tttcccccaa ggcccccggg aaactttctt ctttattgac cacgcttcct
      241 tccgcatcga cggaattaag gaagtggctg acgtgttgat accacaacaa cccctccgcc
      301 aggaccgccg gcgaataaaa ccgtttgggc agggaccaaa ggggtttccc gtcccgcccg
      361 aaggcgtgaa tctccgtcac cgtcacggcg accacccggt ccccccaggg caagaccgtg
      421 acgccgagcg gttgcgtgcc cgcgtcgcag ggctgccgcc acgcaacacg ccccttcgcc
      481 ttcgtggcaa cgtttagaaa ggaattcctt cccgcgttcg catatcgagc ggcatatccg
      541 gtttcttggt tcacggtgct cttcccccct tgggctgacg cagggcccgc cgcgaccggg
      601 cccacgccca ggaccgccac gagcgaccac ccgacaaagg ctgcttttcg aaattgacgt
      661 tttttcatgg gaagggggcg aaggcggcgc ccccgatcac ttcgggcctt tattcacctt
      721 tttgtaggtg gccccgtgtt cgccggaaat ccatccccgg ctaatggcgg accgggcccg
      781 cgtggtgatg tccaaccggt cgccgacggc aatcgcctcc ttggggggaa agtaaaaacg
      841 aaccgtcttc cccttcaact ccggattttc tttaacggcg ggttttgtcc cggggaccgc
      901 ctccttctcc aggggaacgg tcggatcgaa ctccgggatt tcgatcgtcc gagcctcctc
      961 caccgtcaga aggatttcaa gaaaggcccg ttcttccgcc gtcgcgtcgc tggttccgat
     1021 ggtttctttc accgttttcg ccgacgtgac gacccccgtg tattcccgat aggaatccgg
     1081 cccgttgaat ttggcgggcg cggaagaact gacgttttcg ggttccatga tcgaacctcc
     1141 cgtgagaaga atgaggagaa aaagccattt cattcgatgc cccgttcttt ggaaaaaccg
     1201 gcgggtctca aaaacgcgcc gcacgctctt catgcggttc gcccctaagt tcccgaatac
     1261 tcttcggtcg gctgaagcag cagcgctttg aagttttggg tcttcagcgc gttcgcgacc
     1321 cgttcggtcg ccacgagaaa gttggggccg tcttttaaac gaaaaacgct caattcgccg
     1381 acgcgcttcg ggtcgatgac gatggacccc tcgaaacaaa cctcgccgaa gtcgattttt
     1441 ttctccgacc gctcccggtc gatggcgtcg tagaccccac tgaacttgac cgcggaatgg
     1501 tcgcgatact cccggcccgt ggtggggtgc ttaaaaacga ccgggtattg ctgaaaatta
     1561 tcaaccccca attgcttcaa aaacgcgatc aaccgccggg acatgaccgg gagggtcacg
     1621 tcaaaataat ccgcgaattc cgagccgtat tccggcaaaa gctcgcagac gagcggggcg
     1681 gcgggattca ccgtggcgtc cacattgacc caatccgaaa tggtcacctc gctcgccggc
     1741 ggattgaaat caataatatt caacgtctca aaaagatttt gccggacaaa gtaatacatc
     1801 ttcccccctt cgttctcggt ccccccaggc gaaaaccgtg acgcccagcg ggtgcgtgcc
     1861 ggcgtcgcaa gcctgtctcc acgcgacatg ccccttcgcc ttggtggaaa cgttgaaaaa
     1921 ggaattccta cccgcgttcg aaaaccgcgc ggcgtaaccg gattcttggt tcacggtgct
     1981 cttcccccct tgggttgacg cagggcccgc ggcgaccggg cccacgccca ggaccgccac
     2041 gagcgaccac ccgacaaagg ctgctt
//
LOCUS       DAOQLS010000384         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36659_length_2059_cov_3.575349, whole genome shotgun sequence.
ACCESSION   DAOQLS010000384 DAOQLS010000000
VERSION     DAOQLS010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36659_length_2059_cov_3.575349"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PK362_07465"
     CDS             complement(<1..393)
                     /locus_tag="PK362_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC74703.1"
                     /translation="MNAAKGTEPLIRFQKADLGYRGASVLRDVTLDIGEGEAVGLVGP
                     NGAGKTTFLRTALGLLTPRSGEVTRRTGPRFAYVPQADGMDVRWPLTIRQTVALAQKA
                     RRWGGGRSATERRAVEEALERTGVLPIAD"
     gene            complement(390..1307)
                     /locus_tag="PK362_07470"
     CDS             complement(390..1307)
                     /locus_tag="PK362_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNC74704.1"
                     /translation="MIKKIRLLLFGFCLGGSAVQAAGPLRVVATTPDLADLARQVGGT
                     LSTVECLSRGFQDPHFVEAKPSLILKLRNADLFVQTGLDLEVGWAPLLLQGARNPRLQ
                     PGAPGFVDASTFVKILEIPTNLSRAEGDVHPGGNPHYLGDPANAAPVARGLATRMAAL
                     DPTHADVYKKNAEDFVGRLNAKIADWDKRLAPFKGAPFVSYHRNLVYFAAHFGLVSVG
                     EIEPKPGIPPSPSHTSDLIALMKAQKVSLILTMPQYEIRTAEALARATGARVVTIALV
                     PEAVPEAVDYLSAMESNVRGILAAGRSPS"
     gene            complement(1317..>2059)
                     /locus_tag="PK362_07475"
     CDS             complement(1317..>2059)
                     /locus_tag="PK362_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74705.1"
                     /translation="PFELYTEASYALLQDLGATAAPPGHTITVTDQKTGLPVDLALPE
                     DSPTPPKRLGNFAHVSKIRFYKDLSDTTNVDLGFSGALYQPRAVVDGSGAVLQDFDRK
                     KLGAVDFTLRWKPLAQGVYRSAIWRTEILYTDQSLARLLNPVDGSEEAPAARIHRRGG
                     YSYVELQPAKRWRAGLRGDYAEDPTVRSKDTPHIARAIAPYVTFTLTEFNRFRIQWTR
                     KDLPGNEKENLGFLQWTVVLGPHGAHAF"
BASE COUNT          358 a          671 c          670 g          360 t
ORIGIN      
        1 gtcggcgatg ggaaggaccc ccgtgcgctc cagggcctcc tcgacggcgc gtcgctcggt
       61 cgcgctccgg cccccgcccc atcgccgggc cttttgggcc aaggccactg tttgacgaat
      121 ggtcaagggc caacgaacgt ccatgccgtc ggcctggggc acgtaggcga atcgcggccc
      181 cgtccgacgg gtcacctcgc ccgatcgcgg ggtcaagagg cccagggctg ttcgaagaaa
      241 ggtggtcttc cccgcgccgt tgggtcccac gagccccacg gcttcgcctt cgccgatgtc
      301 cagggtcacg tcccgaagaa cgctcgcccc ccggtagccc aggtccgcct tttgaaagcg
      361 gatcaggggt tccgttcctt tcgccgcgtt cacgagggcg accgcccggc ggccagaatt
      421 ccccgcacgt tgctctccat ggccgacaaa taatccacgg cctcggggac cgcttcgggc
      481 accagggcga tggtgaccac ccgggccccc gtggcccgag ccagggcctc cgcggtgcga
      541 atttcatact ggggcatcgt caggatcagg gacacctttt gggccttcat gagcgcgatc
      601 aagtccgagg tgtgggaggg gctcggcgga atgccgggct tcggttcgat ctctcccacc
      661 gacaccaggc cgaagtgggc ggcgaagtaa accagattgc ggtggtaact cacgaagggc
      721 gcgcctttga acggcgccag gcgtttgtcc cagtcggcga tcttggcgtt cagccgaccg
      781 acaaagtcct cggcgttctt tttatagacg tcggcgtggg tcgggtccag ggccgccatt
      841 cttgtcgcca ggccccgggc cacgggcgcg gcgttggcgg gatcccccag gtaatgggga
      901 tttccgccgg ggtggacgtc cccctccgcc cgactcaggt tcgtcgggat ttccaggatt
      961 ttgacgaaag tcgaggcgtc cacgaacccc ggcgcgccgg gttgaagccg cgggttgcgc
     1021 gccccctgca acaggagcgg cgcccagccc acctccagat ccaagccggt ttggacgaag
     1081 agatcggcgt tccgaagttt aagaatcaac gacggtttgg cctccacgaa gtgcgggtcc
     1141 tgaaaacccc gcgacaggca ttccaccgtg gacagggttc cccccacctg acgggccaaa
     1201 tccgccaaat ccggcgtcgt ggccaccacc cgaagggggc ccgccgcttg aaccgcggaa
     1261 ccgcccaggc agaacccgaa caaaagcaac cgaatttttt taatcacgaa atcctcctaa
     1321 aaagcgtggg cgccatgggg gcccaggacg acggtccatt gcaaaaaccc gagattttcc
     1381 ttttcgttcc ccggcaaatc ctttcgggtc cactggattc ggaaccggtt gaattccgtg
     1441 agcgtgaacg tcacgtaggg ggcgatggcg cgggcgatgt gcggcgtgtc cttggatcga
     1501 accgtcgggt cctcggcgta atccccccga agtcccgccc gccacctttt ggccggttga
     1561 agctcgacat aggaatagcc cccgcgccgg tgaatccgcg ccgccggggc ctcttcgctc
     1621 ccgtccaccg ggttcaagag gcgggccagg gattggtcgg tgtaaagaat ttccgtgcgc
     1681 cagatcgccg aacggtagac gccctgggcc aagggtttcc agcggagggt gaaatccacc
     1741 gcgcccagtt ttttccggtc gaaatcctga agcacggcgc cggacccgtc caccacggcc
     1801 cggggctggt acagggcgcc cgaaaatccg agatccacat tggtcgtatc gctcaggtct
     1861 ttgtagaacc ggattttcga aacgtgggcg aaatttccca ggcgcttggg cggggtgggg
     1921 gaatcctccg gcagggccag gtccacgggc aaaccggtct tctgatccgt caccgtgatc
     1981 gtgtgccccg ggggcgcggc ggtcgcgccc aaatcctgga gaagggcgta ggacgcctcg
     2041 gtgtagagtt cgaaggggg
//
LOCUS       DAOQLS010000385         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36828_length_2054_cov_4.027514, whole genome shotgun sequence.
ACCESSION   DAOQLS010000385 DAOQLS010000000
VERSION     DAOQLS010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36828_length_2054_cov_4.027514"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK362_07480"
     CDS             complement(<1..353)
                     /locus_tag="PK362_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74706.1"
                     /translation="MSLLDADTQGLVGPADIADLEDRGHRVVDALGGDVSPTDLTRQL
                     IERLKDEKREQFYSDVLFYLTSERYTENQAYELWKEILDHKFLTSQKLGRNAGIRVAA
                     LDYLLNVRKLILTPR"
     gene            complement(350..907)
                     /gene="dcd"
                     /locus_tag="PK362_07485"
     CDS             complement(350..907)
                     /gene="dcd"
                     /locus_tag="PK362_07485"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNC74707.1"
                     /translation="MTVKSDRWIRRMAQEKGMIEPFAEAQKRDGVVSYGLSSYGYDAR
                     VGDRFKIFTPRPGDVLDPKNAVPAAFVEMTANPYVDLPAHAFALAQTVEYFRIPRNVV
                     TTAYGKSTYARCGLVVNVTPFEPEWEGFVTLSLVNTSDAPVRVYANEGIAQVLFWESD
                     EVCEVSYADRRGKYQKQTAITHARL"
     gene            complement(1048..>2054)
                     /locus_tag="PK362_07490"
     CDS             complement(1048..>2054)
                     /locus_tag="PK362_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74708.1"
                     /translation="AAGLQGAGIEPFFAIAYTRYNAKALDALKKGEKPNDSDVFHEVN
                     PLFREVARRNGYFGLPENELWKKIEANHKSVRGVKEIPERFQKLFASSHDVAVPFHVL
                     IQAAFQTHTDNAVSKTINMPNTATRDDVKEAYWLSYKMGCKGITIYRDGSKSQQVLNL
                     GGAQAEKPKARDASRGMSSEYYEIRTGHGGLHVHIDYDEEGPYRLFTSLSPLGTDISG
                     LTSVVGIMISKYLETGGDPKRILKHLNSVKGDRPFGFGAQRVDSIPHAIAIALRTHLQ
                     KHGKMETPPTEAAGGEKGLELWNRSSALYCPDCFSSNVAQQSGCTGVTCFDCGHSECS
                     "
BASE COUNT          340 a          592 c          708 g          414 t
ORIGIN      
        1 atgcgcggcg tgaggatcag cttccgcacg ttcaacaagt agtccagggc ggcgacccga
       61 ataccggcgt tccgcccgag tttttggctg gtcagaaatt tatggtccag gatttccttc
      121 caaagctcgt aggcctggtt ctcggtgtaa cgctccgagg tgaggtaaaa aaggacgtcg
      181 ctgtagaact gctcgcgttt ttcgtccttc agccgctcga tcaattggcg ggtcaaatcc
      241 gtgggcgaca cgtcgccccc cagggcgtcc accacccggt gaccccggtc ttccaaatcg
      301 gcgatgtcgg cgggccccac caggccctgg gtgtcggcgt cgaggaggct cataggcggg
      361 cgtgggtgat ggccgtttgc ttctggtatt tgccccggcg gtcggcgtag gacacctcgc
      421 agacctcgtc ggactcccaa aagaggacct gggcgatgcc ctcgttggcg tacacccgca
      481 ccggcgcgtc ggaggtgttg accagggaca gcgtcacaaa cccctcccat tccggctcga
      541 agggcgtgac gttcaccacc aggccgcacc gggcgtaggt gctcttgccg taggcggtgg
      601 tcacgacgtt ccggggaatg cggaaatatt cgacggtctg ggccagggcg aaggcgtggg
      661 ccggcagatc cacgtacggg ttcgcggtca tttcgacgaa ggcggcggga acggcgttct
      721 tggggtccag gacgtcgccg gggcggggcg tgaagatttt gaaacgatcg cccacccggg
      781 cgtcgtagcc gtagctggag agaccgtagg agacgacccc gtcgcgcttt tgggcctccg
      841 cgaagggctc gatcatcccc ttttcctggg ccatgcgtcg gatccatcgg tcggacttga
      901 cggtcatgac gactttctcc ttgagaaaaa aaatcccggg agggtttcga aaggaaaacc
      961 cccgcaacgc cctcggggag ggcgacccct cccccggggg gagggccttg tcaacgattc
     1021 gttagcgttc gcccgcggcc cgatggctca cgagcattcc gaatggccgc agtcgaagca
     1081 ggtgacgccg gtgcagccgc tttgttgggc gacgttcgag gaaaaacagt ccgggcagta
     1141 gagcgccgag gagcggttcc acaactccaa gcctttttcg ccgccggccg cttcggtcgg
     1201 cggggtctcc attttgccat gtttttgaag gtgcgtgcgg agcgcgatgg cgatggcgtg
     1261 gggaatggaa tccacccgct gggcgccgaa gccgaagggc cggtcgccct tgacggaatt
     1321 gaggtgcttg aggatccgtt tggggtcccc accggtttcg agatacttgg agatcatgat
     1381 gcccacgacg ctggtcaggc ccgaaatgtc ggtcccgagg ggcgagaggc tggtgaaaag
     1441 gcggtagggg ccttcctcgt cgtagtcgat gtgtacgtgg aggccgccgt ggccggtgcg
     1501 gatctcgtag tactccgagg acatcccgcg ggaagcgtcc cgggccttgg gtttttcggc
     1561 ctgggcgccg cccaggttga gcacctgctg gctcttggag ccgtcgcggt agatggtgat
     1621 gcctttgcac cccattttgt aggagagcca ataggcctcc ttgacgtcgt cccgggtggc
     1681 ggtgttgggc atgttgatcg tcttggacac ggcgttgtcc gtgtgggtct ggaaagccgc
     1741 ttggatcaac acgtggaagg gcacggccac gtcgtgggag gaggcgaaga gcttctgaaa
     1801 gcgctccggg atctccttca cgccgcggac cgacttgtgg ttggcttcga ttttcttcca
     1861 cagctcgttt tccggcaggc cgaaataacc gttgcggcgg gccacttcgc ggaagagcgg
     1921 gttcacttcg tggaacacgt ccgaatcgtt gggcttttcg cctttcttga gggcgtccag
     1981 ggccttggcg ttgtagcgcg tgtaggcgat ggcgaagaag ggctcgatgc ccgcgccctg
     2041 caggccggcc gcca
//
LOCUS       DAOQLS010000386         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36895_length_2052_cov_4.070606, whole genome shotgun sequence.
ACCESSION   DAOQLS010000386 DAOQLS010000000
VERSION     DAOQLS010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36895_length_2052_cov_4.070606"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..171)
                     /locus_tag="PK362_07495"
     tRNA            complement(99..171)
                     /locus_tag="PK362_07495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(136..138),aa:Thr,seq:ggt)
     gene            524..1180
                     /locus_tag="PK362_07500"
     CDS             524..1180
                     /locus_tag="PK362_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009916 - alternative oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HNC74709.1"
                     /translation="MNEPPPGLAAEQAKTLSTPRRPYGALARLLFLTMDLVYGRKRTL
                     SKFKVLEVIARVPYQAWEHVAYIAMTHTYSKPYFARRVFEFIRESRHQQDNEQWHLLI
                     LEEMVQKRKIRENFFLARVVPQILAFVYYHISWLLYVIKPAWSYSLNADFEDHAEHEY
                     MEFVREHPELEVERHESDFQNDYGPFALTADLFRQIALDERLHKEESLARVEGARFPG
                     "
     gene            1318..>2052
                     /locus_tag="PK362_07505"
     CDS             1318..>2052
                     /locus_tag="PK362_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74710.1"
                     /translation="MTDKNRQTSKKYRQRPSPPYHADAFRGEILVGNDGKKYKSVKNA
                     AGIFQWKPAPAHGSLREAFRTLDEEGRPVANLQWPEPLGPFIDQLIRKGAFKKIGSFP
                     VSGRVVVACTIEKGDKLKRATRPAEAGPWAVHYLPRKHHWDPNGGIPQHQGAALAFLE
                     KYRPRDFDKLRWSAGIVPAHSDVGVYAAEYFANEDMVAPDLRAATNDKKALKWVKGTG
                     WKETLARLFYKDEFKKNWYVLSHGALF"
BASE COUNT          455 a          666 c          546 g          385 t
ORIGIN      
        1 tcgtccccac gttcacgtgc ggtttcgtgc gctcaaattt gggttttgcc atggtgtgcg
       61 atctccttgt gtgcgtttgt tgaacgatta aaaaaatcag ctgggaatcg ggattgaacc
      121 gacgacctca cccttaccaa gggtgtgctc taccaactga gctatcccag cgtttaccga
      181 ccgacttcgc cggccccaac aacaaaacgg cgcgggaacc cgcgccgtta aaaaaggttg
      241 aagccgcagg gaattccggt gaagacgggc gcttagaata acatttcgcc gtccgctttt
      301 caacggcggg gggggtcgct ggtcggcgcc ccagaacttt tgccggaagc cttcccgggc
      361 ggaaccgcct aggtcggcct gttaccgccc gaagttctta taaggtccac ccagtttatg
      421 tcttccggac ccgggaggca acgcgccccg gcttaatcca tattcccggg cccgggcacc
      481 ttggatttcg ggcccggcgg cggtcaaatg gtaaaatggg gccgtgaacg aaccgccccc
      541 gggcctcgcg gccgaacaag ccaaaaccct ttccactccc cggcgcccct acggcgcgct
      601 ggcgcgtctg ttgtttttga ccatggacct ggtctacggc cgcaaacgca ccttgagcaa
      661 gttcaaggtg ttggaagtca tcgcccgggt cccctaccaa gcctgggaac acgtggccta
      721 catcgccatg acccacacct actccaaacc ctacttcgcc cggcgggtct ttgaattcat
      781 tcgggaaagc cgccaccaac aggacaacga acagtggcac ctgttgatat tggaggaaat
      841 ggttcaaaag cggaaaatcc gggagaactt ttttctggcc cgggtggtgc cgcaaatttt
      901 ggccttcgtc tattaccaca tcagctggct cctctacgtg atcaaaccgg cctggagcta
      961 ttcgttgaac gccgatttcg aggaccacgc ggagcacgaa tacatggaat tcgtccgcga
     1021 gcacccggag ctggaagttg aacgccacga aagcgatttc caaaacgact acggcccctt
     1081 cgccctgacc gccgacctct tccgccaaat cgccctggac gaacgcctgc acaaggaaga
     1141 aagcctcgcc cgggtggaag gggcgcgatt tcccggttga cccgcccgac ggcgtttcaa
     1201 tccttgccga caaatccccc ctccgagaaa cgccttgaat aaaccccttg gccggacaat
     1261 actatcgagc ggtctttgtt cgacggccgc ggcgtcgccc aaccaaggag cccggtcatg
     1321 accgacaaga accgccaaac atccaaaaaa taccgccagc ggccttcccc cccctatcac
     1381 gccgacgctt tccggggtga aatcctggtt gggaacgacg gcaaaaaata caaatccgtt
     1441 aaaaacgccg ccgggatttt tcaatggaag cccgcccccg cccacgggtc cctgcgggag
     1501 gcgtttcgaa ccctggacga agagggtcgc cccgtcgcga accttcaatg gcccgaaccc
     1561 ctgggtccct tcatcgacca attaatccgc aaaggcgctt tcaagaaaat cgggtctttt
     1621 cccgtgtccg gccgcgtcgt cgtcgcctgc acgatcgaaa aaggcgataa gcttaaaaga
     1681 gccacccgcc ccgccgaggc gggcccctgg gccgtccatt acctgccgcg caagcaccat
     1741 tgggacccca acggcggcat tccccaacat caaggcgccg ccttggcgtt ccttgaaaaa
     1801 tatcggcccc gggactttga caaacttcgc tggagcgccg gcatcgtccc cgcccactcc
     1861 gacgtgggcg tctacgccgc ggaatatttt gccaacgaag acatggtcgc gccggacctt
     1921 cgcgccgcca ccaacgacaa aaaagccctg aaatgggtga agggaaccgg ctggaaagaa
     1981 acgctcgcgc gccttttcta caaggacgaa tttaaaaaaa actggtacgt cctcagccac
     2041 ggcgcgttgt tt
//
LOCUS       DAOQLS010000387         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_36901_length_2052_cov_3.306960, whole genome shotgun sequence.
ACCESSION   DAOQLS010000387 DAOQLS010000000
VERSION     DAOQLS010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36901_length_2052_cov_3.306960"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PK362_07510"
     CDS             complement(<1..596)
                     /locus_tag="PK362_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74711.1"
                     /translation="MRTAALAAFLLPQRLWAVPPMTELIDVPTAEALDHYGYSAGFRF
                     YTDGGLLTKASFGVFPRLNVGFGLDAEGFIGNRSVDLNQPTLNARFRFFDGKRNLPAL
                     ALGYDGQGLFYDHDTDKFRQREKGLYLVGSGEVFVPDLSLHGGVNIYDFKNDYLYTFV
                     GLNYLYADQFGLTVEWDNVRIWRESRLNVGGRYYVTPS"
     gene            complement(845..1195)
                     /locus_tag="PK362_07515"
     CDS             complement(845..1195)
                     /locus_tag="PK362_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019933319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNC74712.1"
                     /translation="MNRRLADEGCRPCDGGASLLAPEELAGLMVSLRLWKLEKIAGVP
                     RIVKTFPFKTFADGMAFARRIADLADAEDHHPTLLVEWGRVTVSWWTHSLGGLHRNDF
                     VMAARTDRILDEGF"
     gene            complement(1192..>2052)
                     /locus_tag="PK362_07520"
     CDS             complement(1192..>2052)
                     /locus_tag="PK362_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC74713.1"
                     /translation="ALVGPNGAGKSTLLKILAGVLPVDQGERVLGLNVQSGYFSQHRW
                     ETLRADRTVLQEASDTKRMNPDLLIRTVLGTFLFRDNAVFKKVAVLSGGEKSRLALAK
                     LLLDPPNLLLLDEPTTHLDMASVEALVEALKSFEGTVAFISHDLYFVNALANHVAHVE
                     NGRVRLYNGNHDDFLRLRPVAESADGAPSPAPARAGDGTPAAGPGWMKSSDDDIRRMR
                     EADKARGKRRKKLNQRLRELEEQVADLKSEIGSVFIQSDYKKLMEIDGALKAAEKELA
                     DTRDALAQDR"
BASE COUNT          383 a          650 c          621 g          398 t
ORIGIN      
        1 aaactgggcg tcacgtaata gcgcccgccc acgttcaagc ggctttcccg ccagattcga
       61 acgttgtccc actccacggt gagaccgaac tggtcggcgt acaagtagtt cagcccgacg
      121 aacgtgtaaa gataatcgtt cttgaaatcg taaatgttca cgccgccgtg caggcttaaa
      181 tcggggacga acacctcgcc gctgcccacc agatacaggc ctttttcccg ctggcggaat
      241 ttgtccgtgt cgtgatcgta gaagagcccc tggccgtcgt agcccagggc cagcgccggc
      301 aggtttcgtt tgccgtcaaa aaagcggaaa cgggcgttca gggtcggctg attgaggtcc
      361 accgaccggt tgccgatgaa cccctcggcg tccagcccga acccgacgtt caagcggggg
      421 aacaccccga aggaggcctt ggtgagcagc cccccgtccg tgtaaaaacg aaaccccgcg
      481 ctgtagccgt aatgatccag ggcctcggcg gtcgggacgt cgatcagttc cgtcatgggc
      541 ggaaccgccc aaagccgttg ggggagcagg aacgccgcca aagcggcggt cctcaaaatt
      601 aaagatcggt ttgacaaatc ggtggacctc tcggtggagg ggcggtggga gccccggact
      661 taaagcgtta ttttaccttt ttccccggtt tcgtcaaagg cgatttggcc tcaccgggct
      721 cttttcgtaa aggcgggaac aggaatttga ttttatgctt attttgcaat gcaaaatcaa
      781 cgcgttgccc gggggcggtc caacggagaa aacccccggc gccccccccg ttccctccgt
      841 cgactcagaa tccctcgtcc aaaatccggt cggtgcgggc ggccatgacg aaatcgttgc
      901 ggtgcagtcc gcccagagaa tgggtccacc aggagacggt gacccggccc cattccacca
      961 agagcgtggg atgatggtct tcggcgtcgg ccaaatccgc gatccgccgg gcgaaggcca
     1021 tgccgtcggc aaaggttttg aagggaaagg tcttgacgat gcgggggacc ccggcgattt
     1081 tctccaactt ccacaaccga agggaaacca tcagccccgc cagctcttcc ggagcgagga
     1141 gcgacgcccc gccgtcgcag ggacggcacc cctcgtccgc caatcggcgg ttcatcggtc
     1201 ctgggccaag gcgtcccggg tgtcggccaa ttccttttcg gcggctttca atgctccatc
     1261 gatttccatg agtttcttgt agtcgctctg gatgaacacc gatccgatct cgcttttcaa
     1321 gtcggccacc tgttcctcca attcccgcag tcgttggttt aatttcttcc gccgctttcc
     1381 ccgggccttg tcggcctcgc gcatgcggcg gatgtcgtcg tccgaggact tcatccaccc
     1441 cggtcccgcc gcgggcgttc cgtcgccggc gcgcgcgggg gccggcgacg gggccccgtc
     1501 ggcactctcg gccaccggcc gaaggcgcag gaaatcgtcg tggttcccat tgtacaaccg
     1561 aactcgcccg ttttccacgt gggccacgtg gttggccagg gcgttcacga aatagaggtc
     1621 gtggctgatg aaggccacgg tgccttcaaa actcttcagc gcctccacca gggcctcgac
     1681 gctggccatg tccaaatggg tggtcggctc gtccagcaat aggaggttgg gcggatccag
     1741 gagaagcttg gccagggcca gacggctttt ttcgcccccg ctcaggacgg cgactttttt
     1801 aaacacggcg ttgtcccgga acaggaaggt ccccaggacc gttcgaatca ggaggtccgg
     1861 attcatccgc ttggtgtcgg aggcctcctg aagcaccgtc cggtcggcac gcagggtttc
     1921 ccagcggtgc tgggagaaat acccggactg cacgttgagc ccgagaaccc gctccccctg
     1981 gtccaccggg aggaccccgg ccaaaatttt caggagcgtg gacttgccgg ccccgttcgg
     2041 gcccaccagg gc
//
LOCUS       DAOQLS010000388         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_37110_length_2046_cov_3.909091, whole genome shotgun sequence.
ACCESSION   DAOQLS010000388 DAOQLS010000000
VERSION     DAOQLS010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37110_length_2046_cov_3.909091"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..238)
                     /locus_tag="PK362_07525"
     CDS             complement(74..238)
                     /locus_tag="PK362_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNC74714.1"
                     /translation="MLKDKALVMKELLAFLWKEKMWWMVPLVAILVLLGAVLFLAQGS
                     ALAPFIYTLF"
     gene            complement(370..1611)
                     /locus_tag="PK362_07530"
     CDS             complement(370..1611)
                     /locus_tag="PK362_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74715.1"
                     /translation="MSAVAIPLANEGGAAEGDRRRARPSKGGESLSRRARLSLFAVLG
                     LGYAARVGWIFQDKASSPHPDLPSFIEPSLRLLNPFDSGMREPGFVWWLWLIRQFGVS
                     GFLGIRLVTALWFIGTAAFVFFLARRLLGEKRAWIVLGFYSFLPTQIQADGLGMRHLV
                     ESFGMAWMLWALLTAEGLSPSRRWVHAAASVALLVMVRISFLGSSALFLLYGALRRRS
                     RALLAALAPALFLLFFHFRANERRFGDPFYTINIHSYWFANAEFIGQPGFPASFEEWQ
                     KDAHRKTLTYRQWMFDRHSPMEIVGAHLVGYYRFFWFSPEKTYFPAWFGPLRWLALGA
                     LLVGFGTAFYSAALRPVLMAMTFYAWPYAFPGHVHWAGRFFVPVSYLFLILIVAGGET
                     LWGFLRIGFASLFVGRSGKGR"
     gene            complement(1608..>2046)
                     /locus_tag="PK362_07535"
     CDS             complement(1608..>2046)
                     /locus_tag="PK362_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC74716.1"
                     /translation="LKALCKEIDILILNDGEARQLSGKPSLIQAGKLIRSWGPRYVVV
                     KKGEHGALLFSERRVSSAPAYPLEEVFDPTGAGDTFAGGFMGYINRHGNRLDETTLHN
                     ALFHGTVMASFTVEKFSLERLLSVSPAQIRARLRLLHRMALPR"
BASE COUNT          514 a          639 c          582 g          311 t
ORIGIN      
        1 ttggtttgga aaccgccgat ggcacgggcc ttggctttcc accaccccca cgccgcccgc
       61 cacacgcgac gcatcaaaac agggtgtaaa tgaaaggcgc cagtgcggat ccctgggcca
      121 aaaacaacac ggccccgagc aggaccagga tcgcgaccaa cggaaccatc caccacattt
      181 tctctttcca gagaaacgcg agcaattcct tcattacgag ggctttgtct ttaagcatca
      241 gcgcacgacc cgacggaatc gattgcggag gacggcccgg tcgaggcgcc gcgccaatac
      301 tccgctgatg ccgttccgcg ttttccctcg acccacggtg tgattttgta ggacttggtc
      361 gacatcgggc taccgccctt tccccgaacg accgacaaag agggaagcga atccaatgcg
      421 aagaaagccc caaagggttt ccccgccggc gacgatcagt atcaggaaaa ggtaggacac
      481 cggaacaaaa aaccggcccg cccaatggac atgccccgga aaagcgtacg gccaggcata
      541 aaatgtcatg gccatcaaca cggggcggag cgccgcggaa tagaaggccg tcccaaaacc
      601 gacgagaagc gcgccgaggg ccagccagcg gaggggacca aaccacgccg ggaaataggt
      661 cttctccggg gaaaaccaaa aaaatcgata gtatccgacc aaatgagctc ccacaatttc
      721 catcggagaa tgacgatcga acatccattg ccgataggtt aaggtttttc ggtgcgcgtc
      781 cttctgccat tcctcaaagg acgccgggaa tccgggttga ccgatgaatt ccgcgttcgc
      841 aaaccaatag ctgtgaatgt tgatggtgta aaaggggtct ccaaaccgcc gctcgttggc
      901 tcgaaaatga aaaaaaagca gaaacagggc cggggcgagg gccgccaaca gggccctgga
      961 gcgccgacgg agcgccccgt aaagcaaaaa aagcgcgctg gatcccagga aactgatccg
     1021 caccatgacc aacagggcga cgctggccgc ggcatggacc caacgtcgag agggcgacag
     1081 tccctccgcg gtcaacaaag cccacagcat ccaggccatc ccgaacgatt caacgaggtg
     1141 gcgcatcccc aggccatcgg cctgaatttg agtgggcaga aacgaataaa acccgagaac
     1201 aatccaggcc cgtttttccc ccagcaaccg tcgagccaga aagaagacaa acgcggccgt
     1261 cccaatgaac caaagcgcgg tgacaagccg aattcccaaa aaaccggaaa cgccgaactg
     1321 ccgtattaac cacaaccacc aaacgaatcc cggctcgcgc atccccgaat cgaaaggatt
     1381 caacaagcga agggaaggct cgataaaact cggcaaatcc ggatgcgggg aggaggcttt
     1441 gtcttgaaaa atccacccga cccgggcggc gtagcccagc cccaatacgg cgaagaggga
     1501 gaggcgcgca cgtctcgaaa ggctttcccc tcccttcgac ggcctcgccc gacgacggtc
     1561 cccctccgcg gcgccgccct cattggcgag ggggatcgcc acggcgctca tcgcgggagc
     1621 gccatccggt gcagcagtcg aagtcgggcc cggatttggg ccggagacac ggacaaaagc
     1681 cgctccaggg aaaatttctc gaccgtgaag ctcgccatga ccgtgccgtg gaacaaggcg
     1741 ttgtgaaggg tggtttcgtc caggcggttg ccgtgccggt tgatgtagcc catgaacccg
     1801 cccgcgaagg tgtccccggc ccccgtggga tcgaacacct cttccagcgg ataggccggc
     1861 gccgaagaca cgcggcgttc cgagaacagc agggcgccgt gttccccttt tttgaccacc
     1921 acgtaacggg ggccccagga gcggatcaat tttcccgctt gaatcaggct gggcttcccg
     1981 gacagctggc gcgcttcccc gtcgttcaaa atcagaatgt cgatttcctt gcacaaggcc
     2041 ttgagt
//
LOCUS       DAOQLS010000389         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_37376_length_2038_cov_5.995966, whole genome shotgun sequence.
ACCESSION   DAOQLS010000389 DAOQLS010000000
VERSION     DAOQLS010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37376_length_2038_cov_5.995966"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="ribD"
                     /locus_tag="PK362_07540"
     CDS             complement(<1..478)
                     /gene="ribD"
                     /locus_tag="PK362_07540"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNC74717.1"
                     /translation="MMSRAIALATRGDRAHPNPRVGCVIARGNRVVGEGAHASFGGPH
                     AEAVALRRAGSRARGATMYVTLEPCPHWGKTPPCAAAVARAGVNRVVAATPDPSRRLA
                     GRGLALLRRAGARVETGLLARRAEALNPGFFRRQRAGRPHVILKMAQTLDGKIASRT"
     gene            700..975
                     /locus_tag="PK362_07545"
     CDS             700..975
                     /locus_tag="PK362_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC74718.1"
                     /translation="MKRRNWESKTKAMIVLQGLKGKPIADLCAEHQISQAQFYQWRDH
                     FLSESHSVFEIADKSRREFALERENARLKHMIGELTMELKKSEFGLL"
     gene            972..1745
                     /locus_tag="PK362_07550"
     CDS             972..1745
                     /locus_tag="PK362_07550"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNC74719.1"
                     /translation="MTYPKVDLRDQDLLASIREIKSEHPFWGYRRVANFLRCVKKVTI
                     NKKRIERLMRRHNLQVKPNLRLKAIRTPMKSKPRASRPNEWWGIDMTKVLIQNFGWVY
                     IVIVLDWYSKKIVGYHADIRSKTEHWLKALDMAVNRQFPDGVRGQDLHLMSDNGCQPT
                     SQKFMEVCSELGIHQAFTSYNNPKGNADTERVNRTMKEELVWLREWTSPFVFFKELET
                     WVEVKYNGLYPHSTNGGIPPNVFELNYFNRHTISRSPAA"
     gene            complement(1723..>2038)
                     /locus_tag="PK362_07555"
     CDS             complement(1723..>2038)
                     /locus_tag="PK362_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74720.1"
                     /translation="LKDAIAKAAHSVFDLVVTDVRLGEGTGFSLYENWSLWSNHPRPS
                     FLFMTGDVLNSALVGDIETRGLHLLHKPIDLATFQRAIRSALAPPSSGVLPPVSRPPG
                     ND"
BASE COUNT          476 a          622 c          564 g          376 t
ORIGIN      
        1 ccgttcgcga agcgatttta ccgtctaagg tctgggccat tttcaaaatc acgtgggggc
       61 ggccggcgcg ttgccgtcgg aaaaatccgg gattcagcgc ttcggcccgt cgggccaaga
      121 gccctgtttc cacccgcgcg cccgctcggc gaaggagggc caggccccgc ccggccaatc
      181 gacggctcgg atccggcgtc gcggcgacca cgcgattcac cccggcccgg gccacggccg
      241 cggcgcaggg aggggttttc ccccaatggg ggcatggctc caaggtcaca tacatcgtgg
      301 cgccccgggc tcgagatccc gcccgacgaa gcgccacggc ttccgcatgg ggaccgccga
      361 aagaggcgtg ggctccctcg ccgaccacgc ggttgccgcg ggcgatcacg caccccacgc
      421 gggggttcgg atgggcgcgg tccccccggg tcgccagggc gatcgcgcgg gacatcattt
      481 tttcatcaag gggacggaac atggccgggg aaaggtggac gtcaacgatt aagaagtgca
      541 atgaacataa aatccccggc cgcgattggg cgataggtct ggacagcggg gctattctag
      601 ctttttgtgg ggtggcggtt caatgctccc gtataccgcc ccccgtttta aggaaggcct
      661 gacgaactac taaaatgtgg cggacaaagg agatccgcca tgaaaagacg caactgggaa
      721 tccaaaacca aggccatgat cgttttacaa ggcctcaaag gcaaacccat cgccgacctc
      781 tgcgccgagc atcaaatctc tcaggcgcag ttttatcagt ggagagatca tttcctctct
      841 gaatcccact ccgttttcga gatcgccgac aaatcccgtc gggaattcgc cctcgagcgg
      901 gaaaacgccc gtttgaagca catgatcggg gaactgacca tggaattaaa aaaaagcgaa
      961 ttcggccttc tatgacctat cccaaagtcg atctccgcga ccaagacctc ttggcctcca
     1021 ttcgggagat caaatccgag caccccttct ggggctaccg aagggtcgcc aatttccttc
     1081 ggtgtgttaa aaaagtcacc atcaacaaaa aacgcatcga acgcctcatg cgtcgccata
     1141 accttcaggt gaaacccaat ctacgcttga aggcgattcg cacccccatg aaatccaaac
     1201 cccgcgcctc tcgccccaac gaatggtggg gcatcgatat gaccaaggtc ctcatccaaa
     1261 acttcggatg ggtctacatc gtcattgtcc tcgactggta ttccaaaaag atcgtcggct
     1321 accacgccga catccgctcc aaaacagagc attggctcaa ggccttggat atggccgtca
     1381 accgccaatt ccccgacggg gtcagagggc aagaccttca tctcatgagc gacaacggct
     1441 gtcagcccac ctcccagaag tttatggagg tttgttccga gctcgggatt catcaagcct
     1501 ttaccagcta caacaatccc aagggcaatg ccgacaccga gcgcgtcaac cgcaccatga
     1561 aagaagagct cgtctggctt cgtgaatgga cgagcccttt tgtgtttttc aaagaactgg
     1621 aaacctgggt cgaggtcaaa tacaacggcc tttacccgca ttcaacgaac ggcggtatac
     1681 cgccaaacgt ttttgagcta aactacttca accgccacac cattagtcgt tccccggcgg
     1741 cctagaaacg gggggcagta cacccgaaga aggcggagcg agggcactgc ggatggcccg
     1801 ctgaaaagtg gccaaatcga tgggcttgtg gaggaggtgg aggccccgcg tttcaatatc
     1861 gccgacgagg gcgctgttca gcacatcgcc cgtcatgaac aaaaacgaag ggcgggggtg
     1921 gttgctccac aggctccaat tttcatacag ggaaaacccc gtgccttccc ccagacgcac
     1981 gtccgtcacc accaggtcaa aaacggaatg ggccgctttc gcgatggcgt ctttgagc
//
LOCUS       DAOQLS010000390         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_37703_length_2029_cov_5.790274, whole genome shotgun sequence.
ACCESSION   DAOQLS010000390 DAOQLS010000000
VERSION     DAOQLS010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37703_length_2029_cov_5.790274"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2029
                     /locus_tag="PK362_07560"
     CDS             <1..>2029
                     /locus_tag="PK362_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC74721.1"
                     /translation="PDVTRALADTFLGLGYLTGPEAAAVTGDQPPLLWGVEDPGLYRD
                     NIAAFENSERTRARVQALAAKLSAAADEARNRFYSPALRALDDHRRAYHDQKEGLAAY
                     AAYLWGLRRGATTEFENTRLLREALQWEERLDFVQVENERRALVEALSRRLPEEALSG
                     LVRESLDYRAGRLSYGDYHRYLRRLCADHGLGLERYPQLSAYVSYVLLAERIDRDGLL
                     QELDALERSAETTLAVSPEEKALVSVRRGLTLLDKLATHGLTPAEWQAYAAEKPRLLA
                     LPQTLSTLVAGPIAGTDALNPQTLDSFERFCSRALDRNGALIKNLLEKMHAEGTSTAV
                     LVAGGFHTAGLAQLCRQQGISYAIVSPRLTKVPAVSRPLDVFARQPLPLEKMLAGEII
                     HLAYPRLGQAAGPGLEAQGSAAARQQTAQILWASLQEYLLSLASHFAPEDRTRYAEFA
                     RQFSTLRGLSADVLNVGGGVRALAVRATAADGQTKEMAILSAPDGGTDAFARIAAAPA
                     FEGRLEMPGGPMTLRVYALSDVATPGFLGRIVWLGQRTRGLAAGNVRPWVDRFSVRLR
                     RMFAPKTAVADTELSLDEAHGRAIAEVSARLGLRAAELEPIARHMRRVRELADRLAKS
                     LEMSEEDIALLRLAASAHDIGKFHEDCVEHVLTPDILAKDSPAFRALAEHER"
BASE COUNT          336 a          716 c          629 g          348 t
ORIGIN      
        1 cccgacgtca cccgcgccct ggcggacacc ttcctggggc tcggttattt gacggggccc
       61 gaagccgccg ccgtgaccgg cgaccaaccg ccgctcctct ggggcgtgga agacccgggg
      121 ctctaccggg acaacatcgc ggcgtttgaa aactcggaac ggacccgggc ccgggtccaa
      181 gccctggcgg ccaagttgtc ggccgccgcc gacgaggcgc gcaaccgttt ttactctccg
      241 gcgcttcggg ccctggacga ccatcgccgg gcctaccacg atcaaaaaga ggggttggcc
      301 gcctacgccg cctatttgtg gggccttcgc cggggagcca cgaccgaatt tgaaaacacc
      361 cggctcctgc gggaagccct ccaatgggag gagcgtctcg actttgttca ggtggaaaac
      421 gaacgtcgcg ccttggtgga agccctttcc cgccgattgc cggaagaggc cctgtcgggc
      481 ctcgtccggg aaagtttgga ttatcgggcg ggccgtcttt cctacgggga ctaccaccgc
      541 tatcttcgcc ggctgtgcgc cgatcacggc ctgggcctcg agcgctaccc ccagctgagc
      601 gcctacgtgt cgtacgtgct gttggccgag cggatcgacc gcgacggcct ccttcaggaa
      661 ttggacgccc tggagcgatc ggccgaaacg acgctggccg tttccccgga agagaaagcc
      721 ctcgtgtccg tacggcgggg cctgaccctt ctcgacaaat tggccaccca cggactgact
      781 ccggccgaat ggcaagccta cgccgcggaa aaaccccggc tcttggccct tccccaaacc
      841 ctttcgaccc tcgtggcggg gcccattgcc gggaccgacg ccctgaatcc ccaaacactc
      901 gattccttcg agcggttttg ctcccgggcc ctggatcgga acggcgcgct cattaaaaat
      961 ttattggaaa aaatgcacgc cgagggaacc tcgaccgcgg tcctggtggc gggcggtttc
     1021 cacaccgcgg ggctcgccca gctgtgccga caacagggaa tttcctacgc catcgtctcc
     1081 ccgcggctaa ccaaggttcc ggcggtatcc cgtcccctgg acgtgttcgc ccggcagccc
     1141 ctgcctctgg aaaaaatgct cgcgggagaa atcatccatt tggcttaccc ccgcctgggc
     1201 caggccgcgg gcccgggtct ggaagcccag ggatcggcgg cggcccgcca gcagacggct
     1261 cagatcctct gggcctcgct ccaggaatac cttttaagcc tggccagtca tttcgccccc
     1321 gaggaccgaa cccgttacgc cgaattcgcc cggcaattct cgaccctccg cggtctgagc
     1381 gccgacgtgc tgaacgtcgg cgggggcgtc cgcgccctgg ccgtccgggc gacggcggcc
     1441 gacgggcaaa ccaaggaaat ggccattttg tcggcccccg atggcgggac ggacgccttc
     1501 gcccgcatcg ccgcggcgcc cgcctttgaa ggacgattgg aaatgccggg cggtcccatg
     1561 actctccggg tatacgcttt aagcgacgtc gccacgccgg gatttttggg ccggatcgtt
     1621 tggctgggcc aacgcacccg gggccttgcc gccgggaacg ttcggccctg ggtggaccga
     1681 ttcagcgttc ggcttcgtcg aatgttcgcc ccgaaaaccg ccgtcgcgga caccgaactt
     1741 tccctcgacg aagcccacgg tcgcgccatc gccgaggtgt ccgcccggct ggggcttcgc
     1801 gcggccgaat tggaacccat cgcccggcac atgcgccggg tgcgggaatt ggccgatcgc
     1861 ctggccaagt ccctggaaat gtccgaggag gacattgccc ttctccgcct ggccgcgagc
     1921 gcccacgaca tcggcaaatt ccacgaagac tgcgtcgagc acgttctcac ccccgatatt
     1981 ctcgccaagg actccccggc ttttcgcgcc ctggccgagc acgaacgat
//
LOCUS       DAOQLS010000391         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_37786_length_2027_cov_4.062880, whole genome shotgun sequence.
ACCESSION   DAOQLS010000391 DAOQLS010000000
VERSION     DAOQLS010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37786_length_2027_cov_4.062880"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..847
                     /gene="cysW"
                     /locus_tag="PK362_07565"
     CDS             56..847
                     /gene="cysW"
                     /locus_tag="PK362_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNC74722.1"
                     /translation="MIAAALTFLGFFLLLPTLAVFVNAFQKGWEAYRDAIADPMSLAA
                     IKLTLIAAGVAVPANVVFGLAASWAIARFRFPGKNFLITLIDLPFSVSPVISGMVFVL
                     MFGAQGWFGPWLADHGVRILFAVPGIVLATVFVTFPFVARELIPLMQSQGTDEEEAAY
                     VLGASGPQMFWRVTLPKIKWGLLYGIILCNARAMGEFGAVSVVSGHIRGRTNTLPLHV
                     EILYNEYNFVAAFAVASLLAFLALLNLAAKTWVEWRFQSDHSDAR"
     gene            854..1363
                     /locus_tag="PK362_07570"
     CDS             854..1363
                     /locus_tag="PK362_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014291385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HNC74723.1"
                     /translation="MNIFVLARDIDRCARDHCDQHVVKMILESVQILCTALNKKGFKT
                     PYRSTHTNHPCVLWAGASYDNFRWLWRLTVALNEEYRFRYRKTVDHASLSVLPRISRF
                     RFPRRGLTPFAQAMPDRYKVPGDAVAAYRQFYAGEKKRFATWTRRSPPRWLGGRARRS
                     SRRSASATH"
     gene            1406..1873
                     /locus_tag="PK362_07575"
     CDS             1406..1873
                     /locus_tag="PK362_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC74724.1"
                     /translation="MERQVFRRGFTLIELMLVVAIIGLLAAIAIPKFANLVTKAKEGA
                     VKGKLGTIRSAAQLYYVDNPEVDSVFTFYVTSFHNFNYLVPKYLDEIPMIEHPTQPDH
                     RRGNGVSTGLIDGLWVGSGWTYTWNSLGGPSALPRIFMNCSHTDSIGRTWSQY"
     gene            1944..>2027
                     /locus_tag="PK362_07580"
     CDS             1944..>2027
                     /locus_tag="PK362_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC74725.1"
                     /translation="MANRRLFLSGFTLIELMLVVAIIGLVAA"
BASE COUNT          341 a          641 c          590 g          455 t
ORIGIN      
        1 cgccgccggg cccgtccacc gtcccctcac ggagccgcgg tgggtccaag tcctactgat
       61 cgccgccgcc ctgacgttcc tgggtttttt cctgttgttg cccacgctgg cggtttttgt
      121 gaacgcgttt caaaagggct gggaggccta ccgggacgcg atcgccgatc ccatgtccct
      181 ggccgccatt aagcttactc tcatcgccgc gggggtcgcg gttccggcca acgtggtttt
      241 tggtctggcc gcctcctggg ccatcgcgcg ttttcgtttt cccggaaaga atttcctcat
      301 cacgctcatc gacctgccct tttcggtctc gcccgtcatc tcgggcatgg tgttcgtctt
      361 gatgttcggg gcccaagggt ggttcggccc ctggctggcg gaccacggcg tgcggatcct
      421 cttcgccgtt ccgggcatcg tgttggcgac ggtgttcgtc acctttccct tcgtcgcccg
      481 ggaactcatc cctttgatgc agtcccaggg caccgacgag gaggaggccg cctacgtgct
      541 cggggccagc ggcccccaga tgttttggcg cgtgaccctg cccaaaatca aatggggtct
      601 tttgtacggg atcatccttt gcaacgcccg ggccatgggg gaattcgggg ccgtgtccgt
      661 cgtgtcgggc cacattcggg gccggacgaa caccctgccc ctccacgtgg aaatcctcta
      721 caacgaatac aatttcgtcg ccgccttcgc ggtggcgtcc ctcctggctt tcctggccct
      781 cctcaacctg gccgccaaaa cttgggtgga gtggcggttt cagtcggacc actccgacgc
      841 gcgctgaatc cccttgaaca ttttcgtgtt ggcccgggac atcgaccggt gcgcccggga
      901 ccattgcgac caacacgtcg tcaaaatgat cttggaaagc gtgcagatcc tctgcacggc
      961 cctcaataaa aaaggattta aaacacccta tcgatccacg cacaccaacc atccctgtgt
     1021 tttgtgggcg ggggcgtcgt acgacaattt tcggtggttg tggcgcctga ccgtggcctt
     1081 gaacgaggaa taccggtttc gctaccgaaa aaccgttgac cacgcgtccc tgtccgtgct
     1141 ccctcgaatt tcccgtttcc gttttccccg ccggggattg acccccttcg cccaggccat
     1201 gcccgatcgg tacaaggtcc ccggggacgc cgtggccgct taccggcaat tttacgccgg
     1261 ggaaaaaaag cgctttgcca cctggacccg acgatcgccc ccgcggtggc tgggggggag
     1321 ggcccggagg tcctcgcgcc gaagcgcttc cgcgacgcat tgaaaagacg cccgggcggg
     1381 gttactctaa tggagaggcc ctcccatgga acgacaagtt tttcggcgcg gatttacctt
     1441 gatcgagctc atgctggtgg tcgccatcat cggactcctg gcggccatcg ccatccccaa
     1501 attcgccaat ctggtaacca aggccaaaga aggcgcggtg aaggggaaac tcgggacgat
     1561 tcggagcgcg gcgcaactct attacgtcga caaccccgaa gtggacagcg tttttacctt
     1621 ttatgtgacc agttttcaca actttaatta tttggtcccc aaatatttgg atgagatccc
     1681 catgatcgaa caccccaccc agccggacca ccggcgcggg aacggcgtca gcacggggct
     1741 gatcgatggg ttgtgggttg gatcgggctg gacctacacg tggaactctt tgggcgggcc
     1801 cagtgcgctc ccgcgcattt ttatgaattg ttcccatacc gacagcatcg gccggacctg
     1861 gagtcaatac tgaccctgcc aaggccttcc gccccggggg ccggtggact tgattgtttc
     1921 ggatcgcggc cctatgctga accatggcaa ataggcgact ttttttgtcc ggttttaccc
     1981 tgatcgaatt gatgttggtg gtggccatca tcgggctcgt ggccgcc
//
LOCUS       DAOQLS010000392         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_37971_length_2022_cov_3.500254, whole genome shotgun sequence.
ACCESSION   DAOQLS010000392 DAOQLS010000000
VERSION     DAOQLS010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37971_length_2022_cov_3.500254"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK362_07585"
     CDS             <1..695
                     /locus_tag="PK362_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNC74726.1"
                     /translation="RFGIMDVDGSMLTPNEFLPLLLDHLVKTRGWTGVAARSVMTSHF
                     LDAVAKRRGLTVQETPVGFKFIGDVMAHENSVYPSRQGHFVLGGEESGGLSIRGHVPE
                     KDGVLGCLLAAEIVAVTKRPLRKSLEILQEEVGTFYTDRLNFHLTMEKMFELRGRLSR
                     NPPTRFGPLAVRRIVETDGFKFILTDGSWIGIRLSGTEPVVRVYLETQDRAKLKALEN
                     AGRVLAGVSTGK"
     gene            779..>2022
                     /gene="eno"
                     /locus_tag="PK362_07590"
     CDS             779..>2022
                     /gene="eno"
                     /locus_tag="PK362_07590"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNC74727.1"
                     /translation="MSAVRAREILDSRGNPTVEVDVLLSDGAFGRASVPSGASTGAHE
                     AVELRDGDKSRFLGRGVLKAVNNVNSVLAPALKGKDPRDQKAVDALMLALDGTENKSK
                     LGANAILGVSMAVARAAADSKKTPLHTHLRQAFGIRSKDYIFPVPLMNILNGGAHADN
                     NVDMQEFMILPVCGTRFRDALRAGAEVFHALKAVLKKNGQNTGVGDEGGFAPNLKSNE
                     EAIQVILQAIQNAGYKAGKDIYLGLDCASNEFHNNGQYRLEGEIAIRDRSADEMIEFY
                     ADWAAKYPIVSIEDGLAEDDWAGWKKLTDRVGKKVQLVGDDLFVTNTKRLEEGIHRGV
                     ANAILVKVNQIGSLTETVDAVQKAQAAGYGTIISHRSGETEDAFIADLAVALNAGQIK
                     TGSASRSERIAKYNQLLRLEESL"
BASE COUNT          419 a          631 c          616 g          356 t
ORIGIN      
        1 accgtttcgg catcatggac gtcgacggtt cgatgctgac ccccaacgaa tttttgcccc
       61 tgctcctgga ccacctggtg aaaacccggg ggtggaccgg ggtcgcggcc cgcagcgtca
      121 tgacgtccca tttcctggac gcggtcgcca aacgccgggg cttgaccgtt caagaaaccc
      181 ccgtgggctt caaattcatc ggcgacgtga tggcccacga aaactccgtg tatccttccc
      241 gccaggggca cttcgtgctg ggcggcgagg aatcgggcgg cttgtccatc cgcggccacg
      301 tgccggagaa agacggcgtt ctcggctgcc tcctggccgc ggagatcgtc gccgtcacca
      361 agcggcccct tcgcaagtcc ctggaaatcc ttcaggaaga ggtggggacg ttttacaccg
      421 accggttgaa tttccatctg accatggaaa aaatgttcga gttgcgcggg cgtctgagcc
      481 gaaacccgcc gacccgcttc ggccccttgg ccgtccggcg catcgtggag acggacggat
      541 tcaaatttat tttgaccgac ggcagttgga tcgggatccg gttgtccggc accgagcccg
      601 tcgtgcgcgt ctatttagaa acccaggacc gggccaaatt gaaggccctc gagaacgccg
      661 gtcgtgtttt ggccggcgtg tcgaccggga aatagggaga ggttccatgg ccaagaaatc
      721 cgtgaaaaaa gcgaagtcgc cctcggcgaa cggttccaaa acgtcgaccg gcgttaaaat
      781 ttcggcggtg cgcgcccggg aaattctgga ttcccgcggc aatccgacgg tcgaagtgga
      841 cgtcctcctt tccgacggcg ccttcgggcg cgcctccgtg ccctccggcg cctccaccgg
      901 cgcccacgag gcggtggagc ttcgggacgg cgacaaatcc cgctttctgg gccgcggcgt
      961 cctcaaggcg gtcaacaacg tcaacagcgt cttggccccc gccctgaagg gcaaagaccc
     1021 ccgggaccag aaagccgtcg acgccttgat gctcgccttg gacggtacgg aaaacaaatc
     1081 caagctcggc gccaacgcga ttttgggcgt ttccatggcc gtggcccggg cggcggcgga
     1141 ttccaaaaaa acgcctctgc acacccattt gcggcaggcc ttcggaattc ggtccaagga
     1201 ttacatcttc cccgtgccct tgatgaatat tttgaacggc ggcgcccacg ccgacaacaa
     1261 cgtggacatg caggaattca tgatcctgcc ggtctgcggc acgcgcttcc gggacgccct
     1321 gcgcgccggg gcggaagtgt tccacgccct gaaagccgtc ttgaagaaaa acggccagaa
     1381 cacgggcgtg ggagacgagg gcggtttcgc tcccaacttg aagtccaacg aagaggccat
     1441 ccaggtcatt ctccaggcca ttcaaaacgc cggctacaag gcgggcaagg acatttacct
     1501 ggggttggat tgcgcgtcga acgaatttca caacaacggc caataccgcc tggaggggga
     1561 gatcgccatt cgcgaccgct ccgccgacga gatgatcgaa ttctacgccg actgggcggc
     1621 caaatacccc atcgtgtcca tcgaggacgg cctggccgag gacgattggg cgggttggaa
     1681 gaaattgacc gaccgcgtgg ggaaaaaggt tcaactcgtc ggcgacgacc tgtttgtcac
     1741 gaacacgaaa cggttggagg aaggcatcca ccggggcgtg gccaacgcga ttctcgtcaa
     1801 ggtcaaccaa attggaagcc tcaccgaaac cgtggacgcg gtgcaaaagg cccaagccgc
     1861 gggctacggg accatcattt cgcaccgatc cggcgagacg gaagacgcct tcatcgccga
     1921 tttggccgtg gccttgaacg ccggccaaat caaaacgggg tccgcctccc gttccgagcg
     1981 gatcgccaaa tacaaccaat tgcttcgcct cgaggagtcc ct
//
LOCUS       DAOQLS010000393         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38160_length_2017_cov_3.974516, whole genome shotgun sequence.
ACCESSION   DAOQLS010000393 DAOQLS010000000
VERSION     DAOQLS010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38160_length_2017_cov_3.974516"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..593
                     /locus_tag="PK362_07595"
     CDS             120..593
                     /locus_tag="PK362_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNC74728.1"
                     /translation="MATTNVLPPAALLRRLAARRRGRRVVFTNGCFDLFHAGHLKVLN
                     LAKRAGDLLVVGLNDDRSVRRLKGPKRPILPLKDRAALMGALKDVDYVTWFGEDTPHH
                     LIRRLKPDVLVKGGDWSADQIVGREFVKKVVRVPLLKGRSTSGIIATIARRYGRG"
     gene            580..1017
                     /locus_tag="PK362_07600"
     CDS             580..1017
                     /locus_tag="PK362_07600"
                     /inference="COORDINATES: protein motif:HMM:NF036812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74729.1"
                     /translation="MAGAKFDGGVLRLLDANLNRAREGLRVLEDTARFRWDDRPMFQG
                     LRRARHGLDRITRRHYPALVASRDAGADPGRRMAEPRRRTDEGLVASNFRRAEEALRV
                     LEEYGKVFAPAAAASFKRLRFELYRWEKSAATRGRARETKTPF"
     gene            995..1654
                     /gene="thiE"
                     /locus_tag="PK362_07605"
     CDS             995..1654
                     /gene="thiE"
                     /locus_tag="PK362_07605"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNC74730.1"
                     /translation="MKRKRLFDLSDVRLYCLTTAPRPGMTYFDMVSKACAGGADAIQL
                     RDKTLSARELLRVAKTLQSVCDQHGALFILNDRVDVALAADVDGIHIGQEDLPVRVVR
                     QMVGHKKLIGCSTHSTAQALQAAGDGADYVSCGPLFPTPTKPDYVPVGLDLVKEYRAL
                     LRIPFVAIGGIDETNVAAVAAAGADRVAVVRAVCGSDRPEEAARRLKEKIIGQREGAR
                     T"
     gene            1651..>2017
                     /locus_tag="PK362_07610"
     CDS             1651..>2017
                     /locus_tag="PK362_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74731.1"
                     /translation="MIRFKRLAIVVLALLLSTAAQWGFSKTVGGLLPRWTGPLLSTGI
                     FLVACRVAVSWLTGVASWRIPLMIYLGFFVATLLSLLAAFLIVPLFMHTGGASGVFVM
                     IGAYALALPISVIGGYVGSR"
BASE COUNT          306 a          644 c          685 g          382 t
ORIGIN      
        1 cgtgtgcctg gacgtcccct cccagcggac ggcgcgcatc caggaaggcc acctcgccct
       61 tctccacacc ctttgcgcca tcgtggaaga cgagctcttc ccctcgaccg ccaaggcgcg
      121 tggctaccac taacgtcctt ccgcccgcgg cgcttctccg ccgcctggcg gcccgtcggc
      181 gcgggcgccg cgtcgttttc accaacggtt gtttcgattt gttccacgcg ggccatttga
      241 aggttctgaa cctcgcgaag cgcgccgggg acctgctggt tgtggggtta aacgacgacc
      301 ggtcggttcg gcggctcaag gggcccaagc ggccgatttt gcccctgaaa gaccgggccg
      361 ccctgatggg ggcgctcaag gacgtggatt acgtcacctg gttcggcgaa gacacgcccc
      421 accatttaat tcgccggttg aaacccgacg tgctggtcaa aggcggggat tggtccgccg
      481 atcaaatcgt cgggcgggaa ttcgttaaaa aagtggtgcg ggtgcccctg ctcaaagggc
      541 gctccacctc ggggatcatc gccaccatcg cccggcgcta tggccggggc taaattcgac
      601 ggcggcgttc tccgcctgct ggacgccaac ttgaatcggg cgcgggaagg cctccgcgta
      661 ttggaagaca cggcccgttt ccgctgggac gaccggccga tgttccaggg gttgcgccgg
      721 gcccgccacg ggttggaccg gatcacccgt cgccattacc cggcgctggt ggccagccgc
      781 gacgccggcg ccgaccccgg ccggcggatg gccgagcccc gacggcgcac cgacgaaggg
      841 ctggtggcgt cgaattttcg ccgggccgag gaagccctcc gggtgttgga ggaatacggc
      901 aaggtgttcg cgcccgcggc ggcggcctct ttcaagcgcc tgcggttcga gctgtatcga
      961 tgggaaaaga gcgcggcgac gcgcgggagg gcccgtgaaa cgaaaacgcc tttttgattt
     1021 gagcgacgtc cggctctatt gcctgacgac cgcgccccgt cccggaatga cctatttcga
     1081 catggtgtcc aaagcctgcg cgggcggcgc cgacgccatc cagttgcggg acaaaaccct
     1141 gtcggcccgg gaattgctgc gcgtggcgaa aacgctgcag tcggtgtgcg accagcacgg
     1201 cgcccttttt atcctgaacg accgcgtgga cgtggccctg gcggccgacg tggacgggat
     1261 tcacatcggt caggaggacc tgccggtgcg ggtggtccgg caaatggtgg ggcacaaaaa
     1321 actgatcggc tgttccaccc attccacggc ccaggccctc caggcggccg gcgacggggc
     1381 ggattacgtg agttgcggcc ccctgttccc gacgcccaca aagcccgatt acgtgcccgt
     1441 gggccttgat ttggtgaagg aataccgcgc cttgttacgg attcccttcg tggccatcgg
     1501 cggcatcgac gaaaccaacg tcgcggccgt ggcggccgcg ggcgcggacc gggtggcggt
     1561 cgtacgggcc gtgtgcggtt ccgaccgacc ggaggaagcg gcccgacggt taaaggaaaa
     1621 aatcatcggt caacgggaag gcgcccggac gtgatccgct ttaagcgcct cgccatcgtg
     1681 gttctggctt tgctgttgtc gacggcggcc cagtgggggt tctcgaaaac cgtgggcggg
     1741 ttattgcccc ggtggacggg ccccctcctc tcgacgggaa tctttttggt cgcgtgccgc
     1801 gtcgcggtgt cctggttgac gggcgtcgcc tcctggcgca tccccttgat gatctacttg
     1861 gggtttttcg tggccaccct tctgtcgttg ctggcggcct tcctgatcgt tcccctgttc
     1921 atgcacaccg ggggcgcttc gggcgtgttt gtcatgatcg gcgcttatgc gttggctctt
     1981 ccgataagcg tgatcggcgg gtatgtggga agccgcg
//
LOCUS       DAOQLS010000394         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38222_length_2015_cov_4.137245, whole genome shotgun sequence.
ACCESSION   DAOQLS010000394 DAOQLS010000000
VERSION     DAOQLS010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38222_length_2015_cov_4.137245"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="PK362_07615"
     CDS             <1..1759
                     /locus_tag="PK362_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC74732.1"
                     /translation="RDHFEWEQQDHREISDEAMLNARLAAAGYERLLEGTRHVLLAFA
                     QLESLQGPDPGAANKILRNFLKRYPTFLNIGVINSDGRLFASALPHDPRLDLSERPYF
                     KYAMASNDFSVGKFQIGSVTHRPSLNCGFPMRDTAGKPRRVVFSALDLKWLNSMAGEV
                     PVPPDARLLLLDREGAVLAVTPPTAAKIGEGYAHWNDLAGENGDGPSPTGGRRLLEDE
                     EHLMTVSPVRLPAGSGLRVVYEISRQAAYRKAHFRLFLNLGLLLLSAFFVLVLGWAAG
                     HLFITRHVTALLKATRRLADGDMNARTGVTGAPGELGQIAGAVDHLADALQKREEELR
                     HTHANLERQVAERTQELRSSNEQLEAFSYSVSHDLRAPLRAIDGFSQILLNEHAENLT
                     PEGKRLLMVVVKNTQQMSHLIENLLTFARLGRQDPHASPVDMAGLAREITDELLVAER
                     HRRTSVEIAPLAPAVGDPAMIQQIWINLLSNALKFTSNTANVFISVGSKTGEGQNTYW
                     IKDNGVGFDPKYAHRLFQVFQRLHSSSEFEGTGVGLAIVQRIVQKHGGRVWAESHPGE
                     GATFYFTLPAGPAALGGKP"
     gene            1756..>2015
                     /locus_tag="PK362_07620"
     CDS             1756..>2015
                     /locus_tag="PK362_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74733.1"
                     /translation="MSPTTAAKWRVLIVEDRPEDRELIEHELTKGKVAFESRATERRD
                     EYLAALKEFKPDVVISDFALPQFDALEALSILRHYQPDIPFLL"
BASE COUNT          398 a          650 c          589 g          378 t
ORIGIN      
        1 tcgggaccat tttgaatggg aacagcagga ccaccgggaa atatccgacg aagcgatgct
       61 caacgcccgt ctggcggcgg cgggatacga gcggcttctg gaaggcaccc ggcacgtgct
      121 cctcgccttc gcccaattgg aaagccttca gggcccggat ccgggagcgg ccaacaagat
      181 cctccgtaat tttctgaaga ggtaccccac cttcctcaac atcggcgtca tcaattccga
      241 cggtcggctt ttcgccagcg cccttcccca cgacccccga ttggaccttt ccgaacggcc
      301 ctatttcaaa tacgccatgg cctcgaacga tttttcggtg gggaaatttc aaatcgggtc
      361 cgtcacccac cggcccagcc tcaattgcgg gttcccgatg cgggacaccg ccgggaaacc
      421 ccgtcgcgtg gttttttccg ccttggattt aaaatggctc aacagcatgg ccggcgaagt
      481 cccggttccg cccgacgccc gcctccttct gctggaccgg gaaggcgcgg tgttggccgt
      541 caccccgccc accgccgcca aaatcgggga aggctacgcg cattggaacg acctggccgg
      601 ggagaacggc gacggacctt ccccaacggg cgggcggcgc ctgctggaag acgaggagca
      661 tttgatgacc gtgtcccccg ttcgtctccc ggcggggtcc gggttgcggg tcgtctacga
      721 aatttcccgg caggccgctt accgaaaagc ccatttccga cttttcctga acctgggcct
      781 gcttctcctt tcggcgtttt tcgtcctggt tctcggatgg gcggccggtc atttgttcat
      841 tacccgccac gtgaccgccc tgttgaaggc gacgcgccgt ctggcggacg gcgacatgaa
      901 cgcgcgcacc ggcgtcacgg gcgccccggg ggagctggga cagatcgccg gcgccgtgga
      961 ccacctggcc gacgcgcttc aaaagcgcga agaggaactt cggcacaccc acgccaattt
     1021 ggagcggcaa gtggccgaac gcacccagga attgagatcc tccaacgagc aactggaagc
     1081 cttcagctac tccgtgtccc acgaccttcg ggccccgctg cgggccattg acggattttc
     1141 gcaaatcctt ttgaacgaac acgcggaaaa cctcaccccg gaagggaagc gtctcctgat
     1201 ggtggtcgtg aagaacaccc agcaaatgag ccatttgatt gaaaacctgt tgacctttgc
     1261 ccgactgggc cgccaggacc ctcacgccag tccggtggac atggcgggct tggcgcggga
     1321 aatcaccgac gaactgttgg tggccgaacg acatcgccgc acaagcgtgg aaatcgcgcc
     1381 cctggccccc gcggtcggcg accccgcgat gattcaacag atctggatca atctgttgag
     1441 caacgccttg aaattcacct ccaacacggc caacgttttt atttccgtcg gatcgaaaac
     1501 cggcgaaggg cagaacacct attggatcaa ggacaacggc gtgggctttg atcccaaata
     1561 cgcccaccgg ttgttccagg tctttcagcg gttgcactcc tcgtccgaat tcgagggaac
     1621 gggcgtcgga ttggccattg ttcagcgcat cgtccaaaaa cacggcggcc gggtgtgggc
     1681 cgaaagccat cccggcgaag gggcgacgtt ctatttcacg ctccccgcgg ggcccgccgc
     1741 ccttggagga aaaccgtgag cccgacgacc gcggccaaat ggcgtgtgct gatcgttgaa
     1801 gaccggcccg aggaccggga attgatcgag cacgaactga cgaagggaaa agtcgccttt
     1861 gaatcccgcg ccacggaacg ccgggacgaa tatttggccg cgctgaagga gttcaagccg
     1921 gacgtcgtga tttcggactt cgcgctcccc caatttgacg cgttggaggc cctctccatt
     1981 ctccgccatt atcaacccga cattccgttt ctgct
//
LOCUS       DAOQLS010000395         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38539_length_2006_cov_4.736545, whole genome shotgun sequence.
ACCESSION   DAOQLS010000395 DAOQLS010000000
VERSION     DAOQLS010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38539_length_2006_cov_4.736545"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK362_07625"
     CDS             <1..201
                     /locus_tag="PK362_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74734.1"
                     /translation="AHLGGPACAVEIRGGSHFVYVHREVAGRQGGTSVQLARIAQATA
                     AFLDREFRGADGPVPADHCRNR"
     gene            264..1595
                     /locus_tag="PK362_07630"
     CDS             264..1595
                     /locus_tag="PK362_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74735.1"
                     /translation="MRRFVFLILLALLPTSLRAGQIHLRYFTLDDVSLVFLVQLVALH
                     PKTHSATVAIKKMWSRNKGRSAPSTLRPSRSNDPADASPGETREVEIYAGVEWKDPRK
                     HVIEEHYWDRDDAPSPGESAFLFFRGTAAEWVAFTAAKEAKLDRFFGEDPAAWDAWID
                     SKTDDERVALYADHDYRAGLLKRFRDRADLPVRFVMTLEDSDLKGELLKTLLAARTPD
                     QREALFQTILDEQTSAGPAARAATLRALYLYDEDLWTTAQKMKLYQIEVDDKDYRENL
                     HRLGYIVQRELRDEKKEKGSLWPYARIMALYWRIHCYNDLDDDLPRILARLTASERKQ
                     FYPEVLRAVAASPWRREYKPYKGGLLRTLLKENDFRSDPALLWKMAEIDFRDGQDAET
                     FRDLLGAMLDLVPASDASARARVKAWADRYLSAPDPFPLYEPLKSRYAARS"
     gene            1773..>2006
                     /locus_tag="PK362_07635"
     CDS             1773..>2006
                     /locus_tag="PK362_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC74736.1"
                     /translation="MKLIVEEGKAGTVLPRVMRAALRLFVEKGINATTIKDIARAGGV
                     SEGALYRHFKSKEDLAFHIFTTHLNDFTMRLAGI"
BASE COUNT          399 a          630 c          589 g          388 t
ORIGIN      
        1 gcccatctgg gcggtccggc ctgcgcggtg gaaattcggg gcggaagcca tttcgtttac
       61 gttcaccggg aagtcgccgg gcgccagggg ggaacctcgg ttcaactggc gcgcatcgcc
      121 caggcgaccg ccgcgttcct cgaccgggaa ttccggggcg ccgacggccc cgtgccggcg
      181 gaccattgcc ggaaccgttg acgcttttcc ggctccgccg atccctcccc gtgggccctt
      241 taaaaagaac tagaatccct ttcatgcggc ggttcgtgtt tttgattctg ctggccctcc
      301 ttccgacctc cctgcgggcg ggacaaattc atctgcgata tttcaccttg gacgacgttt
      361 cgctcgtttt cctcgttcaa ctggtggccc tccaccccaa aacccattcc gcgacggtcg
      421 ccatcaaaaa aatgtggtcc cggaacaaag ggcggtcggc cccgtcgact cttcgcccgt
      481 cccgttccaa cgatccggcc gacgcctccc ccggagaaac gcgcgaggtc gaaatctacg
      541 caggggtgga gtggaaagac ccccgcaagc acgtgatcga agagcattat tgggatcgcg
      601 acgacgcgcc ttcgccgggg gaatcggcgt tcctgttttt ccgcggaacc gcggccgagt
      661 gggtggcctt caccgcggcg aaggaggcca aactcgaccg gttcttcggg gaggatccgg
      721 cggcctggga cgcctggatc gattccaaaa ccgacgacga acgggtcgcc ctctacgccg
      781 atcacgatta ccgcgccggc ctcctgaaac ggtttcgcga tcgcgccgat cttcccgtgc
      841 ggtttgtgat gaccctggag gattcggacc tcaaggggga actcctcaag acgttgctcg
      901 ccgcccggac gcccgaccaa cgggaagcgt tgtttcaaac aattttggac gaacagacgt
      961 ccgcggggcc cgccgcccgg gccgcgaccc ttcgggcgtt gtatttgtac gacgaagatc
     1021 tctggaccac cgcgcaaaag atgaagctct accaaattga agtcgacgac aaggactacc
     1081 gggaaaatct gcatcgattg ggttacatcg ttcagcggga actgcgggac gaaaagaagg
     1141 aaaaaggttc gctttggccc tacgcccgaa tcatggccct ttattggcgg atccactgtt
     1201 acaacgactt ggacgacgat ttgccccgaa ttctggcgcg tttgacggcg agcgagcgaa
     1261 aacaatttta tccggaggtc ctccgcgccg tggccgcgtc cccctggcgg agggagtaca
     1321 agccttacaa ggggggattg ttgaggaccc ttttaaaaga aaacgatttt cggtcggacc
     1381 cggcgctcct gtggaaaatg gccgaaatcg attttcgcga cggccaggac gcggagacct
     1441 ttcgggactt gctgggcgcg atgctcgacc ttgtcccggc ttcggacgct tcggcccgcg
     1501 cccgggtgaa agcctgggcc gaccgctacc tgagcgctcc cgaccccttc ccgctttacg
     1561 aaccccttaa aagccgctac gccgcccggt cctgaatacg aatccgctca ccggcccccg
     1621 ggttttccgc gtcgaggttc ccgggcgggg ccgcgcgcct ttttttgtaa aaacgcccga
     1681 cgtcccttcc aagccaaaac gcacaccccc cttgacaaaa cgaggaatcc cggttatatt
     1741 gtgagtgaac attcacaaac aatggaggga ttgtgaaact gatcgtcgag gaaggcaaag
     1801 cgggaacggt gctcccccgg gtaatgcggg cggcccttcg actttttgtg gaaaagggca
     1861 tcaacgcgac caccattaaa gacattgccc gcgccggcgg cgtgtcggag ggggcgctgt
     1921 accgccattt taaaagcaaa gaagacctgg ccttccacat cttcaccacc catttaaacg
     1981 attttacgat gcgcctggcc gggatc
//
LOCUS       DAOQLS010000396         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38549_length_2006_cov_4.069708, whole genome shotgun sequence.
ACCESSION   DAOQLS010000396 DAOQLS010000000
VERSION     DAOQLS010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38549_length_2006_cov_4.069708"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK362_07640"
     CDS             complement(<1..515)
                     /locus_tag="PK362_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74737.1"
                     /translation="MAPGPRLVGSAAPRALQRLIPRLKLAHLMALPEENFGKLVLTVE
                     RHPLFQKYLYTSDPGRQLFSYGRFQRSGVAPRFLQINEDIAPAAGGLDVESLLEEKRS
                     LISLCRRIGEKNFERYFLYGEGGRSLEDIARECGVSLEDAQRALDLVTRVGVQSEFNE
                     PASPSMSAANTR"
     gene            complement(520..1689)
                     /locus_tag="PK362_07645"
     CDS             complement(520..1689)
                     /locus_tag="PK362_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74738.1"
                     /translation="MSDTPPSLIESPTTSFTNRARPLAMDVAHELSNVLGCLTILPDV
                     MMRHIPPDSPLRRDLMEMRRAVQRLDDLATHLSLLSSDRTHLGPLNLGDLVRVTLAKD
                     PLRLILQDCPSVLTDFRFEDGLPPIQGSADWIPVILRNLVRNALDAMAGGGRLSVSAA
                     FRRLSADHAGLDRVPGGDYAVLSVGDSGPLLTPEQRASLFEPYFSQMVLARPSGGGLA
                     LMVVRRLVRRQGGFVDVRSDPAGSFFDVYFPVIRDAVIRPESPASVKRPRLLIVDDRR
                     DQRELFGRLLENAGHEVVTVSSESEALEALARQAADIVLLDISLRKQHDGFDLYRTLR
                     ARWPGQKVIFVSGHGREEYAETLPDLMEAPFLKKPFSAKTLLATVQDLLASAGPR"
BASE COUNT          398 a          570 c          683 g          355 t
ORIGIN      
        1 cgagtgttgg cggcggacat ggacggggac gccggttcgt tgaattcgga ttgaaccccg
       61 acccgggtca ccaagtccag ggcccgctgg gcgtcttcca ggctcacgcc gcattcccgg
      121 gcgatgtcct ccagggaccg cccgccttcg ccgtataaaa aatagcgttc gaagtttttc
      181 tcgccgatcc gtcggcacag ggagatcagg gaccgttttt cctccagcag cgattcgacg
      241 tccaatcccc cggcggcggg ggcgatgtct tcgttgatct ggagaaatcg gggagcgacc
      301 ccgctgcgct gaaatcgtcc gtaggaaaaa agctggcgcc ccgggtcgct ggtgtagagg
      361 tatttttgaa acagcggatg ccgttcgacg gtgagaacca gcttgccgaa gttctcctcc
      421 ggcagggcca tcaaatgggc gagttttagc cgcgggatga ggcgttggag cgcgcggggg
      481 gccgcggatc cgacaaggcg ggggccgggg gccatgcgat taccgggggc ccgcggaggc
      541 cagcaggtcc tggaccgtgg ccaggagcgt tttggcgcta aagggttttt tgaggaaggg
      601 ggcttccatc aaatccggca gggtttccgc gtattcctcc cggccgtgcc cgctcacgaa
      661 gatcactttt tggccgggcc atcgggcccg aagggtccga taaagatcaa atccgtcgtg
      721 ttgtttgcgg agagagatat ccaggagcac gatgtccgcc gcctggcgcg cgagggcctc
      781 cagggcttcg gattcgctcg ataccgtgac cacctcgtgg ccggcgtttt ccagaagccg
      841 tccgaacaat tcccgctgat cccgccggtc gtccacgatc aagaggcggg ggcgtttcac
      901 cgaggcgggg gactccgggc ggatcaccgc gtcgcggatt accgggaaat aaacatcaaa
      961 gaaggaaccc gccgggtcgg atcgcacgtc cacgaatccg ccctggcggc ggaccagccg
     1021 ccgaaccacc atcagggcca ggccgccccc cgaagggcgc gccagaacca tctgggagaa
     1081 atagggttcg aaaaggctgg cccgttgttc gggcgtgagc agggggcccg aatcgcccac
     1141 gctgagaacg gcgtaatcgc cgcccggcac ccgatcgagc cccgcgtggt cggcggacaa
     1201 acggcggaaa gccgccgata cggaaagccg tccgcctccc gccatggcgt ccagggcgtt
     1261 ccgcaccagg ttgcggagga tcacggggat ccaatcggcc gacccttgaa tggggggaag
     1321 cccgtcttca aatcgaaaat cggtcaggac cgaagggcaa tcctgaagga tcagacggag
     1381 cgggtccttg gccaaagtga cccggaccag gtcgccgagg ttgagggggc cgagatgcgt
     1441 gcggtccgac gagagaaggg acaaatgcgt ggccaaatcg tccaggcgtt gaaccgcccg
     1501 gcgcatttcc atcaggtccc ggcggagggg actgtccggg ggaatgtggc gcatcatcac
     1561 gtcgggcaaa atggtgagac aacccagcac gttgctcaat tcgtgggcca cgtccatggc
     1621 caggggccgg gcccggttgg tgaaggacgt cgtgggggat tcgatcaggg acggcggggt
     1681 gtcgctcaaa ggggcctcca gtttgtcgat aattctaacg agccgtgacc ctcgatatgc
     1741 gattcaacac gccgttgtgt tgaaggatat ttataataca tatttttata tttcccgtaa
     1801 atcctggtca cactataccc atatgcaaaa accatgccaa ccccaggggg ggtgtggttc
     1861 cggcgattcc ttccgatagt gtgaaaaaag ggggaccgcg atatgtggga aagggtcgga
     1921 caatttcggg ggtgggcggt gctttccagg gaaggccacg tgctggaatg gaacgacgag
     1981 gcggagcgcc tttttgggtg gtcccg
//
LOCUS       DAOQLS010000397         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38702_length_2002_cov_3.916795, whole genome shotgun sequence.
ACCESSION   DAOQLS010000397 DAOQLS010000000
VERSION     DAOQLS010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38702_length_2002_cov_3.916795"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..517)
                     /gene="folB"
                     /locus_tag="PK362_07650"
     CDS             complement(173..517)
                     /gene="folB"
                     /locus_tag="PK362_07650"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNC74739.1"
                     /translation="MDKLIIHGLELWLKVGCTEAERAFPQRLEMDVTLEMPLSAAGKS
                     DDLSQSVDYAAVAEALRQKLTPQSFRLIETVAEQAADLVLWKYKPRSVTVLLKKRALP
                     GLAWAGVEIHRP"
     gene            complement(526..1443)
                     /locus_tag="PK362_07655"
     CDS             complement(526..1443)
                     /locus_tag="PK362_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74740.1"
                     /translation="MRPRTLLLIPLLAAPALGAGRMSLKEFNEGREEIRLLTLERVRT
                     VPEDSRAIVTGESRLRELFEEYQVRGGTPPVRLSADTSALRGESQKLIRLRAAGRTPP
                     ADPAVAALVEKRWNLYLAQLDALKETAAADQRAGKSESSVYWAALRAFDRLLEQTDKK
                     LLHNTPPGERSLTDAFARDRTDLLMALESGPKKTRGPVKVVAPEIHAPSMTMDISAFY
                     HAVDSLPEARPLTKGAWTVPVGARTVTERRAGVRLVVRPAAGQGLAYIGSETVDFQYG
                     YLVIPAETAYYFENTGTTPLDIEFVGIKP"
     gene            complement(1445..1840)
                     /locus_tag="PK362_07660"
     CDS             complement(1445..1840)
                     /locus_tag="PK362_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNC74741.1"
                     /translation="MAGNAPLAGPGKTVAVIGATNDRAKFGNKSVRAHLKAGYTVYPV
                     HPRETTVEGLPVFKSIRDVPNVDRVTVYIPPGALLQILPDIAAKQPKELFLNPGTESP
                     EVLEKARSLGLQPIQACSIVDLGYASSHF"
BASE COUNT          368 a          569 c          646 g          419 t
ORIGIN      
        1 ggaaacacca gcagttttgg gtgatcgacc atgacggaca gggaactttt catcaaacgc
       61 caactggttt ggatgcgatt cagaaaggcc atggggaatc ctccgtcggg ggttcgcgag
      121 acaccgatcc cctatgtata gcggcgatcg agggttattt caagcccgaa agttagggac
      181 gatggatttc gacgcccgcc caggcgagcc cgggaagggc gcgcttttta agaaggacgg
      241 tgacggaccg gggtttgtat ttccacagca ccaggtccgc cgcctgttcc gccacggttt
      301 cgatcaagcg aaaggattgg ggtgttaatt tttgccgcag agcctcggcg acggcggcgt
      361 agtccaccga ttggctcaag tcatcgcttt tgcccgccgc gctcaaaggc atttccagag
      421 tcacgtccat ctccaggcgc tgggggaagg cccgctcggc ctcggtgcaa cccactttga
      481 gccagagttc caacccgtga atgatcaatt tatccatgtt ttcctttagg gcttgatccc
      541 gacaaattcg atgtccaggg gggtcgtccc cgtgttttca aaataataag ccgtttcggc
      601 cggaatcacc agatacccgt actgaaaatc gacggtttcg gaaccaatgt aagcgaggcc
      661 ttggcccgcg gccggacgaa ccaccaggcg aacgcccgcc cgccgttccg ttaccgtgcg
      721 ggcgccgacg ggaacggtcc aggcgccctt ggtgagcggg cgcgcctcgg gaagcgaatc
      781 caccgcgtga taaaaggccg aaatgtccat ggtcatggac ggcgcgtgga tctccggcgc
      841 caccactttg acggggcccc gggttttttt ggggcccgat tccagagcca tcaataaatc
      901 ggtccggtcc cgggcgaagg cgtcggttaa acttcgttcc ccgggggggg tgttgtgcag
      961 aagtttttta tcggtttgct ccagcagtcg gtcgaaggcg cggagcgcgg cccagtaaac
     1021 ggaactttcc gattttcccg cccgctggtc ggcggccgcc gtctctttta acgcgtcgag
     1081 ttgggccaag tacaagttcc atcttttttc cacgagcgcc gccacggcgg ggtcggcggg
     1141 gggggtccgt cccgcggccc gcaggcgaat cagcttctgg ctttcgccgc ggagggcgga
     1201 ggtgtcggcg gacaatcgaa cggggggcgt tccgccccgc acctggtatt cctcgaagag
     1261 ctcccgcaac cgggactcgc ccgtgacgat ggcccgggaa tcttcgggca ccgtccgaac
     1321 gcgttccagc gtgagaaggc ggatttcctc ccggccctcg ttgaattctt tgaggctcat
     1381 gcgccccgcg cccagggcgg gggcggcgag cagcgggatc aagagaaggg tccgggggcg
     1441 catgtcagaa atgagaagag gcgtagccca gatcgacgat gctgcaggcc tggattggtt
     1501 ggaggcccaa ggaccgggcc ttttccaaga cttccgggct ttcggtgccg ggattcaaaa
     1561 agagttcctt cggttgcttg gcggcgatgt ccgggagtat ttgcagcagg gctcccgggg
     1621 gaatatagac cgtgacccga tccacgtttg gaacgtcgcg gatggatttg aaaaccggca
     1681 gaccttcgac ggtggtttcc cggggatgga cgggataaac cgtgtagccg gcctttaaat
     1741 gggcgcggac cgacttgttg ccgaatttgg cccgatcgtt ggtggcgccg atgacggcaa
     1801 cggtttttcc gggcccagcg aggggcgcgt taccggccac ggcgtcgttc ccgggcgagg
     1861 ggtcccttca ttgtttttcc tttaagcgtt ttttgaccgc tttttccacc gtcggataaa
     1921 tgttgtcgac aaaaatcccg tatccctcgg cgttgggatg aatcccatcg gataaatttt
     1981 tggacgggtg ggccgcgacc cc
//
LOCUS       DAOQLS010000398         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38708_length_2002_cov_3.611710, whole genome shotgun sequence.
ACCESSION   DAOQLS010000398 DAOQLS010000000
VERSION     DAOQLS010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38708_length_2002_cov_3.611710"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..249)
                     /locus_tag="PK362_07665"
                     /pseudo
     CDS             complement(10..249)
                     /locus_tag="PK362_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
     gene            complement(236..574)
                     /locus_tag="PK362_07670"
     CDS             complement(236..574)
                     /locus_tag="PK362_07670"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNC74742.1"
                     /translation="MRRLIFPGGKTITASPLWKKGLPERLRVYRGNAASNWWNALESD
                     FPLTKKQFSEEAWDGLTRRYFQKQPPGHWELNASLSPFVSFLKQEGVKRWVWELADYE
                     WNDLGIFIGR"
     gene            complement(604..1548)
                     /locus_tag="PK362_07675"
     CDS             complement(604..1548)
                     /locus_tag="PK362_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNC74743.1"
                     /translation="MTTTEFSTLPSLGAGLGLRKEHFKELTPNNKGVGWLEIIAENFM
                     NFGGFPQSVLDYCARHWTIVSHGVNLSIGSVDPLDEEYLDRLKTLLDRVKAPWFSDHL
                     CFTSVGGAYFHDLLPLPFTREAADHVVARVKKVRKKIQRPFLLENPSYYIVPPGADME
                     ETDFYNAVLEEADCGLLLDVNNVYVNSRNHGFDPQEFIRRLPLERVGQIHLAGHRDCG
                     DVVVDTHEGPIIDPVWALYRFTLEALGRPVSTLIEWDTDVPPLPRVTAEADKARGILF
                     GAGWNSASPSPSTSVHSNPSRGSAKRRTLADRREAAPR"
     gene            complement(1607..1939)
                     /locus_tag="PK362_07680"
     CDS             complement(1607..1939)
                     /locus_tag="PK362_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74744.1"
                     /translation="MNLKQLQTLISAGVFSAAVLSGAAYAEDMSAGPASATAPKKTAK
                     AQKMKHKSAAAKKDMAMHHDCAGKNTCKGQGGCATDGSKGKAKMDNMAQHACKGMNAC
                     KGQGGCAS"
BASE COUNT          361 a          588 c          612 g          441 t
ORIGIN      
        1 ggtgagccat cagaagtacc cctcctgctt cttcttctcc atcgaggccg aggcgtcgtg
       61 gtcgatcacc cgtccctggc gctccgatga ggtcgtcagc agcatctcct ggatgatctt
      121 cggcagcgtg tcggccgtgc tctcgatcgc gccggtcagc gggtagcagc cgagcgtgcg
      181 aaagcgcacg gtcttcattt gtggcgtctc cccatcgttc agcggcatgc gctcgtcatc
      241 gaccaataaa aatccccaaa tcgttccatt cgtaatccgc caactcccac acccatcgct
      301 tgaccccctc ctgtttcagg aaggacacga aaggggaaag cgacgcgttc aattcccagt
      361 gccccggcgg ctgtttttgg aaatatcgcc gcgtcaggcc gtcccaggcc tcttccgaaa
      421 actgtttctt ggtcaaagga aaatcgctct ccagggcgtt ccaccaattg gaggcggcgt
      481 tcccgcgata cacccgaagt cgctcgggaa gccccttttt ccaaaggggg gaggccgtga
      541 tcgttttccc gccggggaaa atcaatcgcc gcatttcgtc ctggtaaaat cgcaatccgt
      601 ttttcatcga ggggccgctt cccggcgatc ggccagggtt cttcgtttcg cggatccccg
      661 cgagggattg gaatggacgg aggtcgaggg ggagggggaa gcgctgttcc accccgcccc
      721 gaaaagtatg ccgcgcgcct tgtccgcctc ggccgtgacc cgggggaggg gcggaacgtc
      781 ggtgtcccat tcgataaggg tgctcacggg acggcccagg gcttccagcg taaatcgata
      841 gagcgcccaa acggggtcga tgatgggccc ctcgtgggtg tccacgacga cgtccccgca
      901 atcccggtgg cccgccaggt gaatttgccc cacccgctcc aggggcaacc gccggatgaa
      961 ttcttggggg tcaaacccgt ggttgcggct gttgacgtag acgttgttaa cgtccaacag
     1021 gagaccgcaa tcggcttctt ccaacaccgc gttataaaaa tccgtttcct ccatgtccgc
     1081 gcccgggggc acgatgtagt aggaggggtt ttccaaaagg aagggccgct gaatcttttt
     1141 acgaaccttt ttaacccggg cgaccacgtg gtccgccgct tcgcgggtaa atggaagggg
     1201 caacaagtcg tgaaaatagg ccccgccgac gctggtgaag cacaagtgat cgctgaacca
     1261 tggggctttc acgcgatcga gcagggtttt aagccggtcc agatactctt cgtcaagggg
     1321 atcgacgctc ccgatggata aattgacgcc gtggctcacg atcgtccaat ggcgggcgca
     1381 atagtccaat acggattggg ggaaaccgcc gaaattcatg aagttttcgg caatgatttc
     1441 gagccaaccg acgcccttgt tgttcggcgt cagctcttta aaatgctcct ttcgaaggcc
     1501 gagaccggcc ccgagagagg gaagggtgga aaattcggtt gtggtcatag gggaccctcc
     1561 aaacccgccc cggcccgcgg gccggggcgg gacggcgggg ggtgattcag ctcgcgcagc
     1621 cgccctggcc tttgcaggcg ttcatgcctt tgcaggcgtg ttgagccatg ttgtccattt
     1681 tggcttttcc tttgcttccg tcggtagcgc agccgccctg gcctttgcag gtgtttttgc
     1741 cggcgcagtc gtggtgcatc gccatgtctt ttttcgccgc cgcggatttg tgtttcattt
     1801 tttgggcttt cgcggttttc ttcggcgcgg tggcggaagc cggcccggcc gacatgtctt
     1861 cggcgtacgc ggcgcccgag agcaccgcgg cggaaaagac cccggccgag atcaacgttt
     1921 gcagttgttt caggttcatg ggaactcctt ttgtttggtg gtgatggatc ttgcttctac
     1981 ttcccggggg gaagcaaatc gg
//
LOCUS       DAOQLS010000399         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38819_length_1999_cov_3.400720, whole genome shotgun sequence.
ACCESSION   DAOQLS010000399 DAOQLS010000000
VERSION     DAOQLS010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38819_length_1999_cov_3.400720"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..966
                     /locus_tag="PK362_07685"
     CDS             103..966
                     /locus_tag="PK362_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC74745.1"
                     /translation="MARRGALLLFFSAVLFGVIGALVKRLSLVQPTESLVFFRNAVGL
                     LALLPLMGLWGQDGLATREFGSHAYRALSGLGGMYLSFFALGRMPLADAMALGYTAPL
                     FMPFIARVRLGEAMPRHVAPPLALGFLGVLLILKPGVGVFRPVALAALGSGLLAAVAQ
                     VGIRHLTRTEPSYRIVFYFGLVSTVVSGIPVAFRWRTPPTSLWPMLALVGGLATAAQL
                     LLTAGYRRTSPARGGPMMYVAVATAGAMDWLLWRRLPDALSLAGVAAIVGSGAYILRR
                     SGATVLDGGAA"
     gene            963..1697
                     /locus_tag="PK362_07690"
     CDS             963..1697
                     /locus_tag="PK362_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC74746.1"
                     /translation="MKVRFVLVRPRNPLNIGASSRAMANFGFGDLAVVKPYAPVWREA
                     KSAVGGEPVLKAARDFASVEDALGDCALVVGTTAVRERRTDRPVVSLPDLGDYLRKHH
                     PDIRNGTARAAILFGPEKTGLSGPYLARCQFWLTIPTDPACPSMNLGQAVALCAYEWA
                     RAGGTTAPVNAGTDLPPATIEDRRRLARHAAEAFDAVRFLPFLSAAEKIKKIRQRLLH
                     WNLRRPDVALLHGFFRFVLKDRPRAP"
     gene            1694..>1999
                     /gene="folK"
                     /locus_tag="PK362_07695"
     CDS             1694..>1999
                     /gene="folK"
                     /locus_tag="PK362_07695"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNC74747.1"
                     /translation="MNRERVFIGLGSNVGNRLGHLRKAVEALNRLPGIRVVGKSPVYE
                     TAPVGPRQRDFLNAVAELRSDLAPGVLLASLKGIEKKMGRRVRRRWGPREIDLDLLHF
                     "
BASE COUNT          258 a          689 c          654 g          398 t
ORIGIN      
        1 gctcacggtt cccctgccgc gacttcagga agccctggcc cgggtggccc gggtgaaatg
       61 gtgagggcgg gtctttcctt cccgagccgc tgatcccctt ccatggcccg ccgcggagcg
      121 ttgcttctct ttttttccgc ggtgttgttc ggcgtcatcg gcgccctcgt taaacgattg
      181 tccctcgtac aacccaccga atccctcgtc tttttccgca acgccgtcgg tcttctagcg
      241 ctccttcccc tgatgggcct gtggggccaa gacggtttgg ccacccggga attcgggtcc
      301 cacgcgtacc gcgccctgtc ggggctcggg gggatgtatc tgtccttttt cgccctgggg
      361 cggatgcccc tggcggacgc catggccctg ggctacaccg cgccgctttt catgcccttc
      421 atcgcccgcg tacgactggg ggaagccatg ccgcgtcacg tggcgccgcc cctggccctg
      481 gggtttctgg gcgttctttt gattttaaag ccgggggtcg gggttttccg gcccgtggcc
      541 ctggccgccc tggggtcggg ccttctggcg gccgtggccc aagtcggcat ccgccatttg
      601 acccgcaccg aaccctccta ccggatcgtc ttttacttcg gtctcgtgtc caccgtcgtg
      661 tcgggaatcc ccgtggcctt ccgatggcga acgccgccga cgtccctctg gccgatgttg
      721 gcgctggtgg gcggattggc cacggcggcc caattgcttt tgaccgccgg ctatcggcgc
      781 acttccccgg cccggggcgg gcccatgatg tacgtggccg tggccaccgc cggggccatg
      841 gattggctgc tgtggcgacg actgccggac gccctgtccc tggccggggt ggcggccatc
      901 gtcggatcgg gagcgtatat tttgcggcgg tcgggcgcga cggtcctgga cgggggggcc
      961 gcgtgaaggt tcgatttgtc ctggtgcgtc cgcggaaccc cttgaacatc ggcgcgtcct
     1021 cccgggccat ggccaatttc ggcttcggcg acctggcggt cgtcaaaccc tacgcgcccg
     1081 tgtggcggga ggccaaaagc gccgtgggcg gggaacccgt gctgaaggcc gcccgggatt
     1141 ttgcctccgt cgaggacgcc ctcggggatt gcgccctggt ggtcggaacc acggcggtcc
     1201 gggagcgacg gacggaccgg cccgtggtct ccctgcccga tttgggggat tatttgcgga
     1261 agcaccaccc ggacatccgg aacggcacgg cccgggccgc gatcctgttc ggtccggaaa
     1321 aaacgggcct ctccggcccg tacctggccc ggtgccaatt ttggctgacc atccccaccg
     1381 accccgcctg cccgtccatg aacctgggtc aggcggtggc cctctgcgcc tacgaatggg
     1441 cccgggccgg cggaacgacg gcccccgtca acgccggaac cgatctgccc ccggcgacca
     1501 tcgaggaccg ccggcggctg gcccggcacg cggccgaggc cttcgacgcc gtgcgtttcc
     1561 tgcctttcct ttccgcggcg gagaaaatca agaaaatccg ccaacggctc ctccattgga
     1621 acctccgtcg ccccgacgtg gccctcctcc acggtttttt ccgtttcgtc cttaaagatc
     1681 gtccccgggc gccgtgaacc gggagcgggt cttcatcggt ttgggttcca acgtcgggaa
     1741 ccggctcggc catcttcgaa aagccgtcga ggcgttaaac cgccttccgg gcatccgggt
     1801 ggtgggaaaa tcccccgtct acgaaaccgc cccggtcgga ccgcgccaac gggatttttt
     1861 gaacgccgtg gcggaactgc gctccgatct cgcgccgggg gtcctgctgg cttccctgaa
     1921 agggatcgaa aaaaaaatgg gtcgccgggt tcgccgtcga tggggccccc gggaaattga
     1981 tttggacctg ctccatttc
//
LOCUS       DAOQLS010000400         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38907_length_1996_cov_4.470376, whole genome shotgun sequence.
ACCESSION   DAOQLS010000400 DAOQLS010000000
VERSION     DAOQLS010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38907_length_1996_cov_4.470376"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="PK362_07700"
     CDS             complement(<1..1617)
                     /locus_tag="PK362_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC74748.1"
                     /translation="MERREHINYQDVVRGLIEHGKEAGYLTYEEISQKLPETNFTTEE
                     LDTLFGKLEEMGIDVVERGDDHGRPKADGPEEIGVPELELDTQNSIRMYLSEMGKVPL
                     LTREQEVSLARSIKDNEKILKLVVLESPITHREIQNWEQLLEADEMTPKELMPRGRRT
                     ASELSGMRRRVKTVVQYIIQAELRIDKIKVKLEGKLPAAKKEELREAIDDERREIVNR
                     IISLNLNQEKIKRLVNKIKNLGHKVREYEDEIHRYEKRFKLPYTELKKLYHKALAKQM
                     PSGQFQKLTGYTTTGIESTMQNIETIQLRLVRMARMLPVTPQELLSLDHRIRELEEAI
                     LKDKLQLIKANLRLVVSIAKKHVGSNLELSDLIQEGGLGLIKAVEKFEWKRGFKFSTY
                     ATWWIRQSINRAIADQARTIRIPVHMKEVISKLTKVSRKFRQEMGRDPSVEEYSKALR
                     LSTDKVRQILKIMQEPISLTTPVGEEEDSVLEDFLEDKGDLSPSHAANDALRQQEVEK
                     ALETLSDREAEIIRLRFGIGTGFPRTLEELG"
     gene            complement(1785..1996)
                     /locus_tag="PK362_07705"
                     /pseudo
     CDS             complement(1785..1996)
                     /locus_tag="PK362_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017780641.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          305 a          574 c          648 g          469 t
ORIGIN      
        1 gcccagttcc tccaaggtgc gcgggaagcc cgtgccgatg ccgaagcgca ggcggatgat
       61 ctccgcctcc cggtcgctga gggtttccag ggccttttcg acttcctgtt gacgcagggc
      121 gtcgttggcg gcgtgggagg gcgataggtc gcccttgtcc tccagaaaat cctccaggac
      181 cgagtcttcc tcctcgccca cgggagtcgt gagggagatc ggctcctgca tgatcttcaa
      241 aatctgccgg accttgtccg tcgaaagacg gagggccttg gaatattcct ccaccgacgg
      301 gtcccggccc atttcctggc ggaatttccg gctcaccttg gtcagcttgg aaatgacttc
      361 cttcatgtgc accgggatcc ggatggtccg ggcctggtcg gcgatggcgc ggttgatgct
      421 ctggcggatc caccaggtgg cgtaggtcga gaatttgaag ccgcgcttcc attcgaattt
      481 ttccacggcc ttgatcagtc ccaggccgcc ctcttgaatg aggtccgaca actccaggtt
      541 cgagcccacg tgctttttgg cgatggacac gacgagccgg aggttggcct tgatgagctg
      601 gagcttgtcc ttgaggatgg cctcctcgag ctcccgaatg cggtggtcca aagacagcag
      661 ttcctggggc gtgaccggca acatgcgggc catgcggacc aggcgcagct ggatggtctc
      721 gatgttctgc atggtgcttt cgatgccggt ggtggtgtag cccgtcaatt tttggaactg
      781 acccgaaggc atctgcttgg ccagggcctt gtggtaaagc tttttgagtt ccgtgtaggg
      841 gagcttgaag cgcttctcgt accggtggat ttcgtcctcg tattcccgga ccttgtggcc
      901 caggtttttg atcttgttga ccaggcgttt gatcttttcc tggttcaaat tgagggaaat
      961 gatgcggttg acgatctccc ggcgttcgtc gtcgatggcc tcccgcagct cctctttttt
     1021 ggcggcgggg agtttgccct ccaatttgac tttgatcttg tcgatccgca gttcggcttg
     1081 gatgatgtac tgcacgacgg ttttgacccg ccggcgcatt cccgaaagct ccgacgccgt
     1141 ccggcggccc cggggcatga gctccttggg ggtcatctcg tcggcttcca ggagctgttc
     1201 ccagttttga atttcccggt gggtgatggg ggattccagg acgacgagtt tgagaatctt
     1261 ttcgttgtcc ttgatgctcc gggccaggga cacttcctgc tcccgggtga ggagcggcac
     1321 cttgcccatt tcggacaggt acatgcggat ggagttttgg gtgtcgagtt cgagctccgg
     1381 cacgccgatt tcctcggggc cgtcggcctt ggggcggccg tggtcgtcgc cgcgttccac
     1441 gacgtcgatg cccatttcct cgagcttgcc gaaaagggtg tccagctcct cggtggtgaa
     1501 attggtctcc gggagcttct gggaaatttc ctcgtaggtc aggtagccgg cttccttgcc
     1561 gtgctcgatc aatccccgca ccacgtcctg gtagttgatg tgctcgcgtc gttccatggt
     1621 caccgttccg tcgtagggcc cgcggggtcc gacactgccg ttcgcagttc gcgggtcagg
     1681 gtttggtacc gcaaaatttg ttgttcgtcc cggggcgcgc ggccctcgag catttccagc
     1741 acccggggtt ccagggcggc ccgttcccgc cgccgggcca gcgtcgtgag ccgctcgacg
     1801 ccccggttga aggcgccgaa gaagccgcgc ttctcatggt gatccgggct gaccggacgc
     1861 agcaaggtgg cgcagagtgc gggtgtcagg gtcagggcga ggaaggcgga aatcaggatc
     1921 gagaccgcca tcgacagggt gaactgctgg tagatgaccc ccacggagcc gctgccgaag
     1981 gccatgggaa tgaaca
//
LOCUS       DAOQLS010000401         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_38981_length_1994_cov_3.584838, whole genome shotgun sequence.
ACCESSION   DAOQLS010000401 DAOQLS010000000
VERSION     DAOQLS010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38981_length_1994_cov_3.584838"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /gene="tkt"
                     /locus_tag="PK362_07710"
     CDS             complement(<1..1825)
                     /gene="tkt"
                     /locus_tag="PK362_07710"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNC74749.1"
                     /translation="MATSLSSVDIDALSVNALRFLAVDAVEKANSGHPGTPMEAAPLG
                     HVLWTKILRHNPKNPSWPNRDRFVLSAGHASMLLYGLLHLTGYDLTLEDLKQFRQWGS
                     RTPGHPEFGHTAGVETTTGPLGQGFATGLGMAVAERWLAAKFNRPGFPVVDYRIYAFV
                     SDGDLMEGVASEAASLAGHLRLGQLKYLYLDNRITIEGSTELAFTENVAKRFESYGWR
                     VLSVPDVNDLAAVEAAFRAADAETERPTLIVARTHIGFGSPHKQDSAEAHGAPLGKEE
                     VQLTKKNLGWPGEADFHVPETVRAHMGRTVDRGVALEAEWKAMFERYRLQYPDLATEW
                     EALGRSPFPAGWADRIPVFKAGDGLATRQASGKVLNALAPVLPTLIGGSADLAPSNNT
                     RLEVGGDLTAQNPAGRNIHFGIREHAMGSLLNGLAVDARFLPFGATFLVFSDYLRPAI
                     RLAALMKLGVVYVFTHDSVGVGEDGPTHQPIEHLAALRSIPNVCVLRPADANETAWAW
                     RVALERRTGPTVLILSRQKLPVLDRAVLAPAEGLVRGAYRLTKTPVDPDVLLLASGSE
                     VPLALAAAERLGKEGVRAGVVSFASWELFEAQPETYRREVLP"
BASE COUNT          388 a          629 c          646 g          331 t
ORIGIN      
        1 gtggcaaaac ctcccgccga taggtctcgg gctgggcctc aaaaagttcc caggaagcga
       61 agctcaccac ccccgcccga accccttctt tccccagccg ttcggccgcg gccaacgcca
      121 gcggcacttc ggacccggag gccaacagaa gcacgtcggg atccacgggc gttttggtca
      181 agcgataggc cccccgtacc aggccttccg cgggcgccaa aacggcgcga tccaggaccg
      241 gcaatttttg acgggacagg atcaacaccg tggggcccgt tcgccgttcg agcgccaccc
      301 gccaggccca ggcggtttcg ttggcgtcgg ccggacggag aacgcacacg ttcggaatcg
      361 atcgaagggc cgccaaatgt tcgatgggct gatgggtcgg cccgtcctcc cccacgccca
      421 cgctgtcatg ggtgaacacg tagacgaccc ccaatttcat cagggcggcc aatcgaatcg
      481 ccgggcgaag ataatccgaa aaaaccagaa acgtcgcccc gaagggcaga aaccgggcgt
      541 ccacggccag cccgttgagc agggacccca tggcgtgctc gcggatccca aagtgaatgt
      601 tccgtccggc gggattttgg gcggtcaggt ccccgccgac ctccagccgg gtgttgttgg
      661 acggcgcgag atcggccgat ccgccgatca gggtgggaag aaccggcgcc aaggcgttca
      721 ggacctttcc cgaggcttgc cgggtcgcga ggccgtcccc cgccttgaac accggaatcc
      781 ggtccgccca gcccgcgggg aagggggatc gtcccaacgc ctcccattcc gtggccaaat
      841 ccgggtattg cagacggtag cgctcgaaca tcgccttcca ttccgcttcc agggcgacgc
      901 cccggtcgac ggtccgtccc atgtgcgccc gcaccgtctc gggaacgtga aaatccgcct
      961 ccccgggcca acccaaattc ttcttcgtga gttggacttc ttctttgccc aggggggcgc
     1021 cgtgggcttc ggccgaatcc tgtttatggg ggctcccgaa cccgatgtgg gtgcgcgcca
     1081 caatcaacgt ggggcgttcc gtttcggcgt cggcggcgcg aaaagcggct tcgacggcgg
     1141 ccaaatcgtt gacgtcgggc acagacagga cgcgccatcc gtaggattcg aaccgcttcg
     1201 ccacgttctc ggtaaaagcc aattcggtgg acccttcgat ggtgatccga ttgtccaaat
     1261 aaagatactt cagttggccc agtcgcagat gcccggcgag ggaggccgct tcgctggcga
     1321 ccccctccat caaatcgccg tcgctgacga aggcgtaaat tcgataatcc accacgggga
     1381 aacccggtcg gttgaatttg gccgccaacc accgctcggc gacggccatg ccgaggcccg
     1441 tcgcaaaacc ctgtcccagg ggaccggtcg tggtttcgac tcccgcggtg tggccgaact
     1501 cggggtgacc gggcgtgcgg ctgccccact ggcgaaattg tttaaggtct tccagggtaa
     1561 ggtcgtaccc ggtcaggtgc agcaaaccgt agagcagcat ggaggcgtga ccggccgaaa
     1621 gaacaaagcg gtcgcgattg ggccaggatg gatttttggg attgtgccgg agaattttcg
     1681 tccacaggac gtggcccaac ggggccgctt ccatgggcgt gcccgggtgg ccggagttcg
     1741 ccttttcaac cgcgtccacc gccaaaaaac gcaaggcgtt gacgctcaag gcatcgatat
     1801 cgacggaaga aagtgaagtc gccataacgg aaagctgaat tatacaactt cggggatcgt
     1861 ctgtctgggg gtacgcgggg ttgattaagg gagagaaacg acgccgggaa tcgacgtggg
     1921 acggtcggcg gcgggggcgg aggcgggccg gggggcgacc ggaaggctga aaaagaactc
     1981 gctgccccgg ccgg
//
LOCUS       DAOQLS010000402         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39020_length_1993_cov_3.764190, whole genome shotgun sequence.
ACCESSION   DAOQLS010000402 DAOQLS010000000
VERSION     DAOQLS010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39020_length_1993_cov_3.764190"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK362_07715"
     CDS             <1..387
                     /locus_tag="PK362_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74750.1"
                     /translation="ESHLAACAECRSVRDGWARWRPVLAAAARPADASFSNRVMARVR
                     AEPAPRSGPVVRTFSRIGGLFRPQWMWPLGAAAAALAVMVLAPVRRPAPVSLDTAQFV
                     AELLPAVDETEGGTDDVASDIETYFL"
     gene            401..1063
                     /locus_tag="PK362_07720"
     CDS             401..1063
                     /locus_tag="PK362_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNC74751.1"
                     /translation="MKKIRNARWLSLALGALVLTGASLRAEPSENSGPRGGPGGEMGG
                     GPGGGGERPFEKIKAELGLTDEQAAKLRDHRQAHRQAGMALWQDARAKREALKAELEK
                     PNFDENKVKSLQAAARAAQEKLQDHRLQGIIDVKKILTPEQFAKFQAIMKEKRGQWGN
                     RRGGGFQGSGGVGGRRGGGRFGGRFQNQGQGGGAGPSEGSGSEQGGGPEGGSGPSDSE
                     GF"
     gene            1139..>1993
                     /locus_tag="PK362_07725"
     CDS             1139..>1993
                     /locus_tag="PK362_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC74752.1"
                     /translation="MRSGGVRTAVGLGAILLMGSAPAASGPRTWEDVVRRAAAQNPEL
                     AAARANEDAARARRASSVNGFLPSVSLAQRVSRSDGAERTWSASADAALDLLNVGAWT
                     GVRSADAALREARDQRQLVSADLRLNLFTAFAGLLFAQRQIVVAQKIRDLRNDNAQMV
                     DLKYRSGREYKGNGLRAAAERAQAEADLAQAQRDLSSAQSALSRQMGDETFEVWTVTG
                     TLAVPAASARPNWNALIADHPRVRLAESRVDAARAVYQKARGGLAPTLSVGYSRRYDG
                     PDFFPDGPH"
BASE COUNT          333 a          633 c          725 g          302 t
ORIGIN      
        1 gaatcccatt tggccgcctg cgccgaatgc cggtccgtgc gggacggatg ggcccgttgg
       61 cggcccgtcc tggccgcggc ggcgcggccc gccgacgcct ctttttccaa ccgggtgatg
      121 gcccgggtgc gggccgaacc ggcgccccgg tcggggccgg tcgtccggac cttttcccgg
      181 atcggtggtc tgtttcgacc ccaatggatg tggcccctgg gggcggcggc cgcggccctg
      241 gccgtgatgg tgttggcgcc ggttcggcgg cccgcgccgg tgtccctgga cacggcgcaa
      301 ttcgtggcgg aacttttgcc ggccgtcgac gaaacggaag gcgggacgga cgacgtcgcc
      361 agcgatatcg aaacgtattt tctataaagg gggaacggtt atgaaaaaaa ttcgaaacgc
      421 gcgttggttg tccttggccc tcggggcgct ggttttgacg ggggcgtctc ttcgggccga
      481 accgtcggaa aattcggggc cgcggggcgg gcccggcggg gaaatgggag gcgggccggg
      541 agggggcggg gaacggccct ttgagaaaat caaagccgaa ttgggattga cggacgagca
      601 ggccgccaaa cttcgggacc accgccaggc ccatcgccag gcggggatgg ccctttggca
      661 ggacgcccgc gccaaacggg aagccctcaa ggccgaactg gagaagccga acttcgacga
      721 aaacaaagtg aagtccttgc aggcggcggc ccgggcggcc caggagaaat tgcaggacca
      781 ccggctccag ggcatcatcg acgtgaagaa aattctgacg cccgaacagt tcgccaagtt
      841 ccaggccatc atgaaggaaa aacggggaca atggggcaat cggcgaggcg gcggattcca
      901 agggtccggc ggcgtcggcg gacgtcgggg cggcggccgg ttcggggggc ggtttcaaaa
      961 tcaaggacaa ggcggcgggg ccggccccag cgaaggctcc ggttcggagc aaggcggcgg
     1021 gcccgaaggc gggtccggtc cgtcggacag cgaagggttt taatccgcgc gcggccccgg
     1081 cgattcaacg ccggggccgc cgtgtttttg ggtcggttct ggcatgaagg gggctccggt
     1141 gagaagcggc ggcgttcgga cggcggtggg gctgggagcg atccttctaa tgggatccgc
     1201 tccggcggcg tccggccccc ggacctggga ggacgtggtt cgccgggcgg ccgcgcaaaa
     1261 ccccgaattg gccgccgccc gggcgaacga agacgccgcc cgggcccgac gcgcctccag
     1321 cgtcaacggt tttttgccgt cggtgtccct cgctcagcgc gtctcccggt cggacggcgc
     1381 ggaacggacc tggagcgcct ccgccgacgc cgcgctggac ctcctgaacg tcggcgcctg
     1441 gaccggcgtc cgttcggccg acgcggctct ccgggaagcg cgggaccaac gccaactggt
     1501 ctccgccgat ctccgactaa atttattcac cgccttcgcc gggcttttgt tcgcccaacg
     1561 gcaaatcgtc gtggcccaga aaatccggga cctccgaaac gacaatgccc agatggtcga
     1621 tctcaaatac cgatcgggtc gggagtacaa aggcaacggc cttcgggccg cggccgaacg
     1681 ggcccaggcc gaggcggact tggcccaggc gcaacgggat ttgtcctcgg cccaaagcgc
     1741 gttgagccgt caaatggggg acgaaacctt tgaggtttgg accgttaccg ggacgttggc
     1801 ggttcccgcg gcgtcggccc ggcccaattg gaacgccttg atcgccgatc acccccgggt
     1861 ccgcctggcg gagtccagag tcgacgcggc ccgcgccgtc taccaaaaag cccggggcgg
     1921 tctggccccg acgctgagcg tcggatacag ccggcgctac gacggccccg atttttttcc
     1981 cgacgggccc cac
//
LOCUS       DAOQLS010000403         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39068_length_1992_cov_3.176562, whole genome shotgun sequence.
ACCESSION   DAOQLS010000403 DAOQLS010000000
VERSION     DAOQLS010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39068_length_1992_cov_3.176562"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /gene="pilQ"
                     /locus_tag="PK362_07730"
     CDS             <1..1846
                     /gene="pilQ"
                     /locus_tag="PK362_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNC74753.1"
                     /translation="RAARLLALAAFLFPQPGAFGMAVLESGHLDSTADGTSISSNNEA
                     SLKSISYDRHAVRIKLDKEVAYRVFTLNSPPRVVVELPNTLHGPKPYEASVKDDVLKR
                     IRSSQFKTSPEMVARIVFDLPRMVAFKTVREGDSIVLRFNAGKEVSESADASAREENL
                     QGEAEESLSAAAQVRVGGKSGKRAPKDILSSLPKNPITIDFDDADIRDVIRVLAEMSG
                     INMIYASDLRGFVTVHLDQVPFDEVFSTVLTMQGLVSQQIGNNILRILTPEGLNTDRA
                     RSVTTYRTFFLNYAKAADLGSHLAAVKISPTSRFTVDAPNNALVVTDTPEGLAAAERL
                     ISELDRKPAQVLIETKLVQLSLGKSLDLGIQWEYANSRPLSDRNPANRRIIGQRKTEA
                     GSSPPAGKVGFVGTDATGADEVTTAVDSATRGTGVNLAGPTKAGITFGFINSSDLLTA
                     TLGALETENEAKTLTNPKIITTNNQNAKIQIGQKVPFLQTTVSPGGTATQSTVFADVG
                     FIIDVTPTINEDNRIRLKVKPESSNVEGTTDAGPTINTTTAETEVILRDGDTIVIGGL
                     VSENMVKSVSKVPLLGDLPVLGVFFRSLSDTKKRQELLVFITARIVPD"
BASE COUNT          433 a          625 c          549 g          385 t
ORIGIN      
        1 ccgcgcggcg cgcctcctgg ctctggcggc ttttttgttt ccccaaccgg gcgctttcgg
       61 catggccgtc ctggaatccg ggcacctcga ttcgaccgcg gacggcacgt cgatttcctc
      121 gaacaacgaa gcgagtttga aaagcatttc ctacgatcgt cacgccgtac ggatcaagct
      181 ggacaaagaa gtggcctatc gggttttcac cctcaattcg cccccgcggg tcgtcgtgga
      241 actaccgaac acccttcacg gccccaaacc ctacgaagcc tccgttaagg acgacgtcct
      301 aaagcgcatt cgcagctcgc agttcaaaac gtcccccgag atggtggccc ggatcgtttt
      361 tgacctcccc cgcatggtgg ccttcaaaac cgtgcgcgaa ggggacagca tcgtgctccg
      421 gttcaacgcg ggaaaagaag tttccgaatc ggccgacgcg tcggcccgag aggaaaatct
      481 tcagggggaa gccgaggaat ctctgtcggc cgccgcgcag gttcgcgtgg gcggaaaatc
      541 cggcaagcgg gcgcccaagg acatcctttc gagtttgccc aaaaatccca tcaccattga
      601 ttttgacgac gccgacatcc gcgacgtcat tcgtgttttg gccgaaatga gcggcatcaa
      661 tatgatctac gcctccgacc ttcggggatt cgtcaccgtc cacctggatc aagtcccgtt
      721 cgacgaagtg ttcagcaccg tcctcacgat gcaaggtctt gtttcccagc aaatcggaaa
      781 caacatcctt cgaattttga cgcccgaagg tctgaacacg gatcgggccc gatccgtgac
      841 cacctatcga acgtttttct tgaactacgc caaggcggcg gatctggggt cccatttggc
      901 cgcggtgaaa atttcgccga cttcccgttt taccgtggat gccccgaaca acgccttggt
      961 ggtgacggac acgccggaag gtttggccgc ggccgagcgg ttgatttccg aattggatcg
     1021 gaagcccgct caggtgttga ttgaaacgaa attggttcag ctttccctcg gaaaatcttt
     1081 ggatctgggt atccagtggg aatacgcgaa cagccggccg ttgagcgacc gaaatccggc
     1141 aaatcgtcga atcatcggcc agcgaaaaac cgaagccggt tcctctcccc cggccggtaa
     1201 agtgggattt gtgggcacgg acgccacggg cgccgacgag gtgaccaccg ccgtggattc
     1261 ggccacgcgc ggcaccggcg tcaacttggc cggtccgacc aaggccggga tcaccttcgg
     1321 ttttattaac agctccgatt tgttgacggc gaccctgggc gctcttgaaa ccgaaaacga
     1381 ggcgaaaacg ctcaccaacc ccaaaatcat caccaccaac aaccagaacg ccaaaattca
     1441 aatcggtcaa aaagtaccct ttcttcaaac gacggtgtcc ccgggcggca cggccacgca
     1501 gagcaccgtt tttgccgacg tcggctttat cattgacgtg acgccgacga tcaacgaaga
     1561 caaccggatt cgcctgaaag tgaagcccga atccagcaac gtggaaggca ccaccgacgc
     1621 cggaccgacc atcaatacca ccaccgccga gaccgaagtg attttgcggg acggcgacac
     1681 catcgtcatc ggcgggttgg tcagcgaaaa catggtgaag tccgtcagca aagttcctct
     1741 cctgggcgat ctcccggtgt tgggcgtttt cttccgaagt ttgtcggaca ccaagaagcg
     1801 gcaggagctt ttggtgttca tcaccgcgcg catcgtcccc gactgaccca tcgtcggcgc
     1861 ctccccgctt agcgcccatg tcgcccgaag cgtccccttc cctcgaggtc ctgctcaagg
     1921 aggcggacaa cgcccgccag cgccaatggt gggacgaagc ggttaaactc caccaggcgg
     1981 cgctcaaggc ct
//
LOCUS       DAOQLS010000404         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39124_length_1990_cov_4.325065, whole genome shotgun sequence.
ACCESSION   DAOQLS010000404 DAOQLS010000000
VERSION     DAOQLS010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39124_length_1990_cov_4.325065"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1743
                     /locus_tag="PK362_07735"
     CDS             1..1743
                     /locus_tag="PK362_07735"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNC74754.1"
                     /translation="MLQKTPAPVTLPDYVEEEIRRFETNIRELRAGRMDMDDFRRFRL
                     NNGIYGIRNQTDKQMIRIKVPFGQLTPDQLDALADVTETFAPSKAGHWTTRQNLQIHM
                     IPLEDTPKVMRRVAESGLTPREGCGNTVRNITANPYTGVQPGEVFDVAPYADAAFRYF
                     LRNPLSANLPRKFKMAFESTDADYALTAIHDMAFVAEIRNGVRGFRTYVGGGLGATPQ
                     VAIRLEEFMPADLLLPSIEAVIRLFDRNGERQDKLHARIKFLVKKWGADEFRKKFLAE
                     RKAVMGTRGGTIDWSFPVVEEAAPPVPTHVAAVTPTAGFDRWKSTNVGAQKQAGYSWA
                     TVVLPLGDITAVQMRQLAEVSRRLNGGRLRTTIEQNFLLRWVKNEHLPALYNELVKIG
                     LGEAGAGRFSDITRCPGADTCQIAVTKSRELAKALGGLFNDGLKADADMEGLHVKISG
                     CTNSCGQHHIGTLGFYGTYRKVGDRPVPHYMVLVGGSTKEGEVKFGQPVGAIPARRVP
                     EAVKKLVALFKAEKQGAENFAQWLERVGKARVKSEIQEFTTLPPFEQNPSLYYDWGEN
                     AEFKVDIGVGECAA"
     gene            1805..>1990
                     /locus_tag="PK362_07740"
     CDS             1805..>1990
                     /locus_tag="PK362_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNC74755.1"
                     /translation="MILPEVGGKGQQALAAARVALVGLGGLGSPAAFYLAGAGVGTLG
                     LIDDDTVDVSNLHRQPLH"
BASE COUNT          414 a          642 c          601 g          333 t
ORIGIN      
        1 atgcttcaaa aaacccccgc ccccgtgacc ctgccggatt acgtcgagga agaaatccgc
       61 cgttttgaaa cgaacatccg cgaactgcgg gccgggcgca tggacatgga cgattttcgc
      121 cggttccgtt tgaacaacgg catttacggg atccgcaacc aaaccgacaa acagatgatc
      181 cggatcaaag tgcccttcgg ccaactgacg cccgatcaat tggacgccct ggcggacgtc
      241 acggaaacct ttgcgccctc caaagcggga cactggacga cccgccaaaa ccttcaaatt
      301 cacatgatcc cgctcgagga cacgccgaag gtcatgcggc gtgtggccga atcgggactg
      361 accccccggg agggttgcgg aaacaccgtc cggaacatca cggccaaccc ctacaccggc
      421 gtgcaaccgg gagaggtctt cgacgtggcc ccctacgccg acgcggcgtt tcggtatttt
      481 ttacggaacc cattgagcgc caatcttccc cgcaagttca agatggcctt tgaatccacg
      541 gacgcggatt acgccctgac ggccattcac gacatggcct tcgtggccga aattcgcaac
      601 ggcgtgcggg gatttcgaac ctacgtgggc gggggcttgg gcgccacgcc tcaggtggcc
      661 atccggctgg aggaattcat gccggcggac cttcttctgc cctcgatcga agccgtgatc
      721 cggcttttcg accggaacgg cgaacgccag gacaaattgc acgcccggat caaatttctg
      781 gtgaaaaagt ggggcgccga cgaattccgc aagaaattcc tggccgagcg caaagcggtg
      841 atgggaacgc gcggcggaac gatcgattgg tccttcccgg tggtcgaaga agcggcgccc
      901 ccggtgccga cccacgtcgc cgcggtcacc ccgacggccg ggttcgaccg ttggaaatcc
      961 accaacgtcg gcgcccaaaa acaggccggc tacagctggg ccacggtcgt gctccccctg
     1021 ggggacatca cggccgttca aatgcgtcaa ttggcggagg tttcccgacg gctgaacggc
     1081 ggccgactgc ggacgaccat cgagcaaaat ttcctgcttc gttgggtcaa aaacgaacac
     1141 ctccccgcgc tctacaacga actcgttaaa atcggcctgg gcgaagccgg cgccggccgg
     1201 ttttccgaca tcacccggtg ccccggagcg gacacctgcc aaatcgccgt gaccaaatcc
     1261 cgggaattgg ccaaggccct gggcggcctg ttcaacgacg ggttgaaagc cgacgccgac
     1321 atggaaggct tgcacgtcaa aatctccggc tgcacgaact cttgtggcca gcaccacatc
     1381 ggcaccctgg ggttctacgg cacttaccga aaagtggggg accgcccggt gccccattac
     1441 atggtgctgg tcggcggctc caccaaggaa ggggaagtga agttcggcca acccgtgggg
     1501 gcgattcccg cccgtcgggt tcccgaggcg gtgaaaaagc tggtcgctct attcaaagcg
     1561 gaaaagcagg gcgccgaaaa cttcgcccaa tggctggagc gcgtcggaaa ggcccgggtc
     1621 aaatccgaaa tccaggaatt cacaaccctt ccgcccttcg agcaaaaccc ttccctctat
     1681 tacgattggg gcgaaaacgc cgaattcaaa gtggacatcg gcgtcgggga atgcgccgcc
     1741 tgaggacgtc gcgcggcgtc gcttttccgt gacggaaatc gacctcgccc gctacgcccg
     1801 ccaaatgatc ctccccgagg tgggggggaa gggccagcaa gccctggcgg cggcccgggt
     1861 ggccttggtc ggcctgggcg gtttgggatc gcccgccgcc ttttacctgg ccggggcggg
     1921 cgtcgggacc ctgggattga tcgacgacga cactgtcgat gtctccaacc ttcaccgcca
     1981 gcccctgcac
//
LOCUS       DAOQLS010000405         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39191_length_1988_cov_3.873771, whole genome shotgun sequence.
ACCESSION   DAOQLS010000405 DAOQLS010000000
VERSION     DAOQLS010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39191_length_1988_cov_3.873771"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PK362_07745"
     CDS             complement(<1..696)
                     /locus_tag="PK362_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNC74756.1"
                     /translation="MNIVVVGHVDHGKSTVVGRLFADTGSLPEGKLEAVRKECGRTGK
                     PFEYAFLLDALTDEQDQGITIDTARSFFKTAKRDYIIIDAPGHIEFLKNMISGAARAE
                     AAVLVIDAKEGVRENSRRHGYLLSMLGIRQIIVCVNKMDLVDRSESHFKKIETEYREF
                     LAGIGAAAPRQFIPISAVNGENLAQRSPSMPWYKGPTVLESLDAFEKAPSKSDRPFRM
                     PVQWVNRPNQSFRG"
     gene            complement(744..1646)
                     /gene="cysD"
                     /locus_tag="PK362_07750"
     CDS             complement(744..1646)
                     /gene="cysD"
                     /locus_tag="PK362_07750"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNC74757.1"
                     /translation="MDNLDQLEQQSVYIFREAFRHFERLCMLWSIGKDSTVLLWLARK
                     AFFGHVPFPLVHIDTSYKLPEMITYRDRLAMEYKLTMVVGQNVDVIKSGRTFPATQHL
                     PDGHPDKVSRQECCGLLKKDALTNTLNGTWPRQKLNLKTGKYEIDPDRAPYHGVIVGA
                     RADEEGSRSKERYFSPRDKNNNWDIGEQPPEFWNQFKTDFAPGTHVRIHPLLDWTELH
                     IWEYIKREKIPTVSLYYDQGAGTRYRSLGCGPCQAPIQSASKNVDEIIAELKSGKLKN
                     IAERSGRLQDGKHGLEELRREGYM"
     gene            complement(1799..>1988)
                     /locus_tag="PK362_07755"
     CDS             complement(1799..>1988)
                     /locus_tag="PK362_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74758.1"
                     /translation="SARLVALLAEMQSLQAKLNADIAAVQEKKIPYKEQMRQLTDLQA
                     AFRVRKLALVDEISALNK"
BASE COUNT          325 a          557 c          666 g          440 t
ORIGIN      
        1 gccgcggaac gactggttcg gccgattgac ccactggacc ggcatgcgga agggccggtc
       61 gcttttggag ggagcctttt caaaggcgtc cagggactcc aacaccgtgg gccctttgta
      121 ccagggcatg gaggggctcc gctgggccag gttttcgccg ttcaccgcgc tgatcgggat
      181 gaattggcgc ggggcggcgg cgccgatccc ggccaggaat tcccggtact cggtttcgat
      241 ttttttaaag tggctttcgc tccggtccac caaatccatt ttgttgacgc agacgatgat
      301 ctggcggatg cccagcatgg agagcagata gccgtggcgg cggctgtttt cccgcacgcc
      361 ttccttggcg tcgatgacca ggacggcggc ctcggcgcgg gcggccccgg agatcatgtt
      421 tttcaaaaat tcgatgtggc ccggggcgtc gatgatgatg taatcgcgct tggcggtttt
      481 aaagaaggac cgggcggtgt cgatggtgat gccctggtcc tgctcgtcgg tcagggcgtc
      541 cagcaaaaag gcgtattcaa agggcttgcc cgtgcgcccg cattccttcc gcacggcctc
      601 gagtttcccc tcgggcaggg accccgtgtc ggcgaagagg cggccgacca cggtgctctt
      661 cccgtggtcc acgtgaccca cgaccacgat gttcatctgc tcccgcaggg ccgtcggcgc
      721 ggcttgggct ttggcggagg cgctcacatg tacccttccc gacgcaactc ctccaggccg
      781 tgcttgccgt cctgcaggcg gccggagcgc tcggcgatgt tcttgagctt gccggattta
      841 agttccgcga tgatctcgtc cacgttcttg gaggcgcttt gaatgggcgc ctggcagggg
      901 ccgcagccga ggcttcggta gcgggtgccc gcgccctggt cgtagtacag ggacaccgtc
      961 ggaatctttt cccgcttgat gtattcccag atgtgcagct cggtccagtc cagcagggga
     1021 tggatgcgca cgtgggtgcc gggggcgaaa tcggttttga actggttcca aaattccggt
     1081 ggctgttcgc cgatgtccca gttgttgttc ttgtcccggg gcgaaaaata acgctctttg
     1141 gagcggctgc cctcctcgtc ggcccgggcc cccacgatga cgccgtggta gggcgcgcgg
     1201 tccgggtcga tctcgtattt gccggtcttc aaattcagtt tttgccgggg ccaggtgccg
     1261 ttcagcgtgt tggtcagggc gtctttttta agcaggccgc agcattcttg gcgggacacc
     1321 ttgtccgggt gcccgtcggg caggtgctgg gtggcgggaa aggtccgacc ggatttgatg
     1381 acgtcgacgt tttggccgac gaccatcgtg agcttgtatt ccatggccag gcggtcccgg
     1441 taggtgatca tttcgggcaa tttgtagctg gtgtcgatgt ggaccagggg aaaggggacg
     1501 tggccgaaga aggctttccg ggccagccag agaaggacgg tggagtcctt gccgatggac
     1561 caaagcatgc aaagccgctc gaagtgccgg aaggcttcgc ggaaaatata aacgctttgt
     1621 tgttccagtt ggtccagatt gtccattgaa agttggagcg cggtaggcag aagggttatt
     1681 ttacaaaaat tggacggacg ccgggaagtc cggcccggga ggccgcgcgg gggtcggcgg
     1741 ccccgtttcg aactcccccc gggacggggc ggtcaatggg cgcaaacgga atcggttttt
     1801 atttgttcaa cgcggagatt tcgtccacga gggccaactt ccggacccgg aaagccgcct
     1861 gaaggtccgt caactgccgc atttgctcct tgtacgggat ttttttctcc tgcaccgcag
     1921 cgatgtcggc gttcaatttg gcctgaaggc tttgcatttc cgccaacaat gccaccaggc
     1981 gggcggat
//
LOCUS       DAOQLS010000406         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39259_length_1986_cov_4.227861, whole genome shotgun sequence.
ACCESSION   DAOQLS010000406 DAOQLS010000000
VERSION     DAOQLS010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39259_length_1986_cov_4.227861"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PK362_07760"
     CDS             complement(<1..1052)
                     /locus_tag="PK362_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74759.1"
                     /translation="MKRIPPLPFLAAAILLAGLIARFSPGPMPVWLLALEVILTVLAL
                     FVFGSIPYRLDKNALTFGAGMIVAATFFTRWWPASVLREEWTARGLSALGEFVARHFL
                     HFRALDGLIHLDTMLFIVGLTYFVSAISQSRLLETVAFGLLTRWRGRVFPTVGLLAGI
                     VALASGILDGVSMIGLLIRTLVILLFWAKVKDRDILFAVMLATVITTVSGMWLAYGEP
                     PNLIMKANLHPLLTDGFFLRYCLPGALASYLLVLLRLRPVLRDKVVNFRRLDVLDRHT
                     ADVRFLQADRHGDVVLPVEFIEEHRGEIGTHYPAVVERLHRGVPFGEALVNERVPRDT
                     RLRLLGRFLSEDLAETL"
     gene            complement(1475..>1986)
                     /locus_tag="PK362_07765"
     CDS             complement(1475..>1986)
                     /locus_tag="PK362_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNC74760.1"
                     /translation="TGRWRYALPVILALGLLGVARVGGRDVLGPGNAVAQLLTHGDLT
                     GWHPSFPVAKIAGTLLTYWSGIAGGIFAPSLSIGAALGANAAQWMGLSVALCALLGMA
                     AFLSAVIQAPITAFVIIFEMSGHHDNLLPVMLAALIAYMTARVVGAKNLYQTLSLNYA
                     FLLETPPPK"
BASE COUNT          475 a          583 c          621 g          307 t
ORIGIN      
        1 agggtttccg cgagatcctc cgatagaaac cgtccgagga ggcgaaggcg ggtgtcccgg
       61 gggacccgtt cattgaccaa ggcctccccg aaggggacgc cccggtggag ccgctcgacg
      121 acggccgggt aatgggtccc gatctccccg cggtgttcct cgatgaattc cacgggcaac
      181 accacgtcgc cgtgacggtc ggcctgcagg aatcggacgt cggccgtgtg gcgatccagt
      241 acgtccagcc gccggaaatt gaccactttg tcccggagca ccggacgcag tcgaaggagc
      301 accaataggt agctcgccaa ggcccccggc aggcagtaac gcagaaaaaa accgtcggtc
      361 aagagggggt gcaaattggc tttcataatc aagttgggcg gttcgccgta ggccaaccac
      421 atgccggaga ccgtggtgat gaccgtcgcc aacatgaccg cgaaaaggat gtcccgatcc
      481 tttactttcg cccagaacag taagatgacc agagtccgga tcaagaggcc gatcatggag
      541 accccgtcca gaatgcccga agccagggcg acgattccgg ccaatagacc gacggtggga
      601 aaaacccgtc cccgccaacg agtcaacaaa ccgaaggcca cggtttccag caggcgggac
      661 tgggaaatgg ccgaaacaaa ataggtgagg ccgacaataa agagcatcgt gtccaaatgg
      721 atcaggccgt ccagcgcgcg gaaatgcaga aaatgccggg cgacgaattc cccaagggcc
      781 gatagccccc gcgccgtcca ctcttcccgc aggaccgacg cgggccacca gcgggtgaag
      841 aacgtggccg caacaatcat tccggccccg aacgtcaggg cgtttttatc caatcggtaa
      901 ggaatcgatc cgaaaacaaa tagggcgagc accgttaaaa taacttccag agccaagagc
      961 caaacgggca tggggccggg ggagaaccgg gcaatgaggc ccgccaaaag aatggcggcg
     1021 gccaaaaagg gaaggggggg gattcgcttc attgtccccg gaccccggcg actttaaaat
     1081 ggagagcgca acggcgatgg tcaaccccgc cggccctcca acccatccgc ccgtataaaa
     1141 atgggaacac cgggtccgtt tttacaggat gtattggcaa tcggtacccc ccccaaaaaa
     1201 aaggaccgcc attccgcctc ccccccatgg tttcccatcg gggacgagcg gaatggcggc
     1261 ccgaccattc ttatcctccg gaaccgacat cgattccgaa cccttttata attgactgca
     1321 acgcagtata ccagttgctt tgcgatcatg ggtgtcgatg aaaggcgttc ggggaattcg
     1381 cggtcctcaa tgtcggattg gtaaaacccg tcgaaggaaa ggatcaggac cccttcgcag
     1441 aacggttcag cgcgttccct cgttggcgcg ttcgctattt cggtgggggg gtctcgagga
     1501 ggaaggcgta gttcagggaa agggtctgat ataaattttt ggccccgacg acgcgggccg
     1561 tcatgtaggc gatcaaggcc gcgagcatga cgggcaagag attgtcgtgg tggcccgaca
     1621 tctcgaagat gatgacgaag gcggtgatgg gcgcctggat gacggccgac aggaaggcgg
     1681 ccatacccaa aagggcgcag agggcgacgg agaggcccat ccactgggcc gcgttggccc
     1741 ccagtgccgc cccgatggat agggatggag cgaaaatgcc ccccgcgatg cccgaccagt
     1801 acgtgagcag ggtccccgcg attttggcca cgggaaagga agggtgccaa ccggtcaggt
     1861 cgccgtgcgt gagcaattgg gcgacggcgt ttccgggccc caggacgtct cgaccgccaa
     1921 ctcgagccac ccccaaaaga cccagggcga gaatcaccgg gagggcataa cgccaacgac
     1981 cggttt
//
LOCUS       DAOQLS010000407         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39260_length_1986_cov_4.224236, whole genome shotgun sequence.
ACCESSION   DAOQLS010000407 DAOQLS010000000
VERSION     DAOQLS010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39260_length_1986_cov_4.224236"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PK362_07770"
     CDS             <1..1253
                     /locus_tag="PK362_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC74761.1"
                     /translation="LQVTQRPQELKDREGEIDKVTKDKEAAIAAQEYEKAAKLRDKEK
                     EMRRQMEEARKSWREKRDATVPVVAGEDIAAVVSKWTGIPTTKLTETEQEKLIHMEAE
                     LHKRLIGQQEAIVSISQAIRRSRTGLKDPKKPIGSFIFLGPTGVGKTELARALAEFLF
                     GNEEALIRIDMSEYMEKFAVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLLDEIEK
                     AHPDTFNILLQMMDDGALSDNLGHRVSFKNTVIIMTSNVGARLISKGKSLGFLVQEDA
                     AKDYAAMKDTVMEEVKKAFNPEFLNRLDDIIVFHPLTREDSRKILDLMLARVSKKLAL
                     QGFTVDLTEEARAFLVDKGFDPNYGARPLQRSIQRLLEDPLAEDILSRKIAAGTSVFV
                     DLDSQGQKLSFSTTPPQKAVKSGG"
     gene            1317..>1986
                     /locus_tag="PK362_07775"
     CDS             1317..>1986
                     /locus_tag="PK362_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74762.1"
                     /translation="MRWRNRGALVALLGLSLFALWWGMGVPRVERRLQSKLESTLARV
                     LGQPVRSDAVRLSPLFLHFSVENLRVGDPAAPLFACRRWTFHTAIADETPWSWMIFSL
                     GRSDVEVPVARWRSGGETPTWPADLWSRLPRHSFRWVHGAFLWERGSETPIRFADVSG
                     GVDLGTDGIAWNLRGGGDPGPWSVSGALKNAGEGRSTGSGRLVLDNFPADYWARALPP
                     AAGRW"
BASE COUNT          391 a          646 c          601 g          348 t
ORIGIN      
        1 gtctgcaagt gacccagcgc ccccaggagt tgaaggaccg cgaaggggaa atcgacaaag
       61 tcaccaagga caaggaagcc gccatcgccg cccaggaata cgaaaaggcc gccaagctgc
      121 gcgacaagga aaaggaaatg cgccgccaaa tggaagaggc ccggaagagc tggcgcgaaa
      181 aacgggacgc cacggtgccc gtggtcgccg gcgaggacat cgccgccgtc gtttcgaaat
      241 ggaccggcat tcccaccacc aaattgacgg agacggagca ggaaaagctg atccacatgg
      301 aggccgagct gcacaagcgc ctgatcggcc aacaggaagc catcgtgtcc atcagccagg
      361 ccatccggcg gagccgcacc ggcttgaagg acccgaaaaa acccatcggc agctttattt
      421 tcctgggccc caccggcgtt ggaaaaaccg aattggcccg ggccctggcg gagttccttt
      481 tcggcaacga agaagccctg atccgcatcg acatgtcgga atacatggaa aaattcgcgg
      541 tgtcgcgcct catcggcgcg cccccgggat acgtgggcta cgaggagggc ggccaactga
      601 cggaagccgt ccgccggaaa ccctattcgg tcgtgctctt ggacgaaatc gaaaaagcgc
      661 acccggacac cttcaacatt ctgctccaaa tgatggacga cggcgccctg tccgacaacc
      721 tgggccaccg ggtgagtttc aaaaacaccg tcatcatcat gacctccaac gtgggagcgc
      781 ggttgatttc caaggggaaa tccctggggt tccttgttca ggaggacgcc gccaaagatt
      841 acgccgccat gaaagacacg gtcatggagg aagtcaaaaa ggcgttcaat ccggaattct
      901 tgaaccggtt ggacgacatc attgttttcc atccgctgac ccgggaggac agccgcaaaa
      961 ttctggacct tatgctggcg cgcgtgtcca agaaactcgc ccttcagggg ttcaccgtgg
     1021 atctcacgga agaagcgcgg gctttccttg tcgacaaagg gttcgacccc aactacgggg
     1081 cccggccgtt gcagcggtcc attcagcgcc ttctcgagga tcccctggcc gaggacatcc
     1141 tgtcgcggaa aatcgccgcg gggacgtcgg ttttcgtgga tttggattcc caaggccaaa
     1201 agctctcctt ctccaccacg cccccccaaa aagccgtcaa aagcggcgga taagcggcga
     1261 ccggccgggc cggaatcgcc gccccgggcg tccgcctctt cccacggggt ttattcatgc
     1321 gatggcgaaa tcggggcgcg cttgtcgccc tcctcggcct ttccctcttc gccctctggt
     1381 gggggatggg cgtccctcgc gttgaacgac gtctccaatc caaactggaa tccaccctcg
     1441 cccgggtact gggccaaccc gtccggtccg acgccgtccg tctttcgccc ctcttccttc
     1501 acttctccgt cgaaaacctc cgcgtcggcg accccgccgc tcccctcttc gcctgccgcc
     1561 gttggacgtt ccacaccgcc attgccgatg aaacgccctg gtcctggatg attttttccc
     1621 tcggacgatc ggacgtggag gtccccgtgg cccggtggcg gtcgggcggg gaaacaccga
     1681 cctggcccgc cgatttgtgg tcccgccttc cgcgtcattc cttccgttgg gttcacggcg
     1741 cgttcctttg ggaacgcggg agcgaaacgc cgatccgctt cgccgacgtg tccggcgggg
     1801 tggatctcgg gaccgacggg atcgcttgga accttcgggg cgggggcgat ccggggccct
     1861 ggtccgtctc tggcgcgctt aaaaacgcgg gggagggacg ttcgacggga tcgggacgcc
     1921 tcgttctcga caatttcccg gcggactact gggcccgggc ccttccgccc gccgccggtc
     1981 gatggg
//
LOCUS       DAOQLS010000408         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39302_length_1985_cov_3.681865, whole genome shotgun sequence.
ACCESSION   DAOQLS010000408 DAOQLS010000000
VERSION     DAOQLS010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39302_length_1985_cov_3.681865"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1985
                     /locus_tag="PK362_07780"
     CDS             12..>1985
                     /locus_tag="PK362_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74763.1"
                     /translation="MSEFRPKFIEAVVDDRYAVMPNITQAIIASVKSEFAAEKANVVV
                     VESREEKGAARIQIYDDAGNFLGWVLMRTSNTNPVLSMKGQGRDAATLARLKRILKNA
                     LENDQFTGRFNQAEIDKGLGTVVPGSQAGFAQVSLLTLLASGLGAVVLALTGAPGVLV
                     GFALVPGLLAAGMEAVRWWASGRGAPGRKSIAATPEREILEAAGVRRALTTPKVSVSP
                     EMDKRQLQGGYKQDGTGVPVGAADDGSRARGENAGNQGYYAQGGRESFLKVYRDMRNF
                     FENRAARHGGNPVRLVIKPGIGGQHTPFQGLASVFEVVDPSSGKIVGEYELGKDFAAS
                     LEAAVKDLRIGWDQVAVIPSSKSGSTDETMMIFVELLSVLIEKQSSKAFADLFLKTLH
                     DLNFPDGANERPGKDLFKVDVKENGFATTSLVELLSHRASQAGLDVNVREVLGKVLGN
                     MFFETTDRPEQSRLSAFIRNSGLDRDLGADAPGFGAMFDNVGGRWTADLHMLTFLAYH
                     RLDAEAYWTARDAGIAEVVAGTHKAVEIGNRIVDLEIADIALVVPNDLYWYAKSIEQN
                     FNESLWPADAVNERRTPNLVAVRASDWEAQRANYEGRSDVMIIDAVTKDVPPITEENQ
                     IYLGAVLNRAAGRAALPGMFAQLATTFYGITETV"
BASE COUNT          393 a          601 c          645 g          346 t
ORIGIN      
        1 ggtatccgga aatgagcgaa tttcgcccga aattcatcga agcggtcgtc gacgatcgct
       61 acgccgtcat gccgaacatc acccaggcga tcatcgcctc ggtcaaaagc gagttcgcgg
      121 ccgaaaaagc caacgtcgtg gtggtcgaaa gccgggagga aaaaggcgcg gcgcggattc
      181 agatttacga cgacgccgga aacttcctgg gttgggtgct gatgcggacg tccaacacca
      241 accccgtgct ttcgatgaaa ggtcaggggc gggacgccgc cacgctggcc cgactgaaac
      301 ggattttgaa gaacgccctg gagaacgacc agttcaccgg gcgctttaac caagcggaaa
      361 tcgataaagg actggggacg gtcgttcccg ggtcccaagc cggttttgcc caggtgtcgt
      421 tgttgacatt gttggcctcg ggattgggcg cggttgtcct ggccttgacc ggcgctcccg
      481 gcgtcctggt gggattcgcc ctcgttccgg gtttgctggc ggcggggatg gaagcggttc
      541 gttggtgggc ttccggtcgg ggagccccgg gacgaaaatc catcgcggcc acgccggagc
      601 gggaaatttt ggaggcggcc ggcgttcgcc gggcgctgac cacgcccaag gtttcggtgt
      661 cgccggagat ggacaaacgc caactgcagg gcggttacaa acaggacggc acgggcgttc
      721 ccgtgggagc cgcggacgac ggatcccggg cccgcggcga aaacgccggc aaccagggct
      781 attacgccca gggcggccgc gagtcgttcc tgaaggtcta ccgcgacatg cggaatttct
      841 ttgaaaatcg cgcggcccga cacgggggga accccgtccg attggtcatt aagccgggca
      901 tcggaggcca gcacaccccg ttccaggggt tggccagcgt gttcgaggtg gtggacccct
      961 cctcgggcaa aatcgtgggg gaatacgagc tcggaaaaga tttcgccgcg tccctcgagg
     1021 ccgcggtgaa ggaccttcgg atcggatggg accaggtggc cgtgattccc agttcgaaat
     1081 ccggttccac cgatgaaacc atgatgatct tcgtggaact tctttcggtg ctgatcgaaa
     1141 agcagtcttc caaggctttt gcggatctgt tcctgaaaac cctgcacgac ctcaacttcc
     1201 ccgacggcgc caacgaacgc ccgggcaagg acctctttaa ggtggacgtg aaggaaaacg
     1261 gcttcgccac gaccagcctg gtggagctgc tgtcccaccg ggcctcccag gccgggttgg
     1321 acgtcaacgt gcgggaggtc ctcggcaagg tcctcggcaa catgttcttc gaaaccacgg
     1381 accgaccgga acaaagccgc ttgtcggctt ttatccgcaa ctccggtctg gaccgggacc
     1441 tcggggccga cgcccccggg ttcggcgcca tgttcgacaa cgtgggcggc cgctggacgg
     1501 cggacctgca catgctgacg ttcctggcct atcaccgcct ggacgccgaa gcctattgga
     1561 cggcccggga cgcgggcatc gccgaggtcg tggcgggaac ccataaagcg gtggagatcg
     1621 ggaatcggat cgtcgatctc gagatcgcgg acatcgccct ggtggttccc aacgaccttt
     1681 attggtacgc caaatccatc gaacagaatt tcaacgaaag cctctggccc gcggacgccg
     1741 tgaacgaaag acggaccccg aacctcgtgg ccgtgcgcgc ctccgattgg gaagcccagc
     1801 gggccaacta cgagggccgg agcgacgtca tgatcatcga cgccgtgacc aaggacgttc
     1861 cgcccatcac cgaagaaaat caaatctatc ttggggccgt cttgaaccgg gcggccggtc
     1921 gagcggccct ccccggcatg ttcgcccaat tggcgaccac gttctacggg atcacggaaa
     1981 ccgtc
//
LOCUS       DAOQLS010000409         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39536_length_1979_cov_4.263514, whole genome shotgun sequence.
ACCESSION   DAOQLS010000409 DAOQLS010000000
VERSION     DAOQLS010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39536_length_1979_cov_4.263514"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..583
                     /locus_tag="PK362_07785"
     CDS             104..583
                     /locus_tag="PK362_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC74764.1"
                     /translation="MRSARAFKGFTLIELMLVVAVIGLLAAIAIPKFADLVDAAREAS
                     LKGYIGNLRAALRMYYADTEGIYPYSPTGFSDPNFGLYQYIDQNKVMPPPRVPKSISD
                     NHAFPLYFTSQLVNQFFPMTVGDNPSISYFKNTDNNSADMLVACFHPDRRGLTWSRF"
     gene            933..>1979
                     /locus_tag="PK362_07790"
     CDS             933..>1979
                     /locus_tag="PK362_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNC74765.1"
                     /translation="MMSKKMVTIDGNEGVCHVAYKLNEVIAIYPITPSSGLSERADLL
                     ASMKKPNLWGEVPTVVEMQSEGGAAGAVHGALQTGSLSTTFTSSQGLLLMIPNMYKIA
                     GELTSTVFHIAARSIAAQGLSIFNDHQDVMAVRQTGWAILSSNSVQEGMDMATIAQAS
                     TLLARVPFVHFFDGFRTSHEIQKVELVSDEVLGAMIDTKLINEHRSRGLSPEHPFIRG
                     TAQNPDVYFQARETVNPYYKKVPGIVQAEMDKYAKLTGRQYKIYDYYGHPEADRVLVL
                     MGSGCESVSEVVDYLNARGEKVGMLKVRLFRPFSAEHFVNALPKSVKQIAVLDKTKEP
                     GSQGEPLYLDVSSAI"
BASE COUNT          427 a          606 c          517 g          429 t
ORIGIN      
        1 caaccgtttt cccggatttc ccgggggtcg gcccaccggc ctcggtaccc cgtcctgggg
       61 accattgatt gttgtttttt ccgggcctat actcgaaggc aggatgagat ccgccagagc
      121 tttcaagggt tttaccctca ttgagttgat gttggtggtg gccgtcattg gccttttggc
      181 ggccatcgcc attccaaaat tcgccgacct ggtcgacgcc gcccgggaag caagcctcaa
      241 aggatacatc ggaaatcttc gcgcggccct tcgaatgtac tacgcggaca cggaggggat
      301 ttatccctat tccccgacgg ggttctccga tcccaatttc ggtttgtatc aatacatcga
      361 ccaaaacaag gtgatgcctc cgccgagggt tccgaaatcg attagcgata accatgcctt
      421 tccgctgtat tttacatcgc agttggtgaa tcaatttttc ccgatgacgg tgggcgacaa
      481 cccctccatc agttatttta aaaacaccga caacaattcg gccgatatgt tggtggcgtg
      541 ttttcatccg gatcgacgcg gtttaacctg gagccgtttt taatctttcg gtcgtttgtc
      601 gccgtctccc ccccatgcct ccgcttcggt cagcgccttg atcatggggg gccttccccg
      661 ctgtcgaacc aaagaattgg gggcacgctt gaatatatga caaatttggt atactattaa
      721 cagggaaaga aaacgctccc agggaaatag cgggaaacag ggcaaatttt caaggcttct
      781 tgacagtttc ggtcggcggt tggtatactt gcctcgaaac aagcgcacta cggcctttcc
      841 tctcttgcta taagagccat cccctcaaga agtgacgaca aggacgtcgg gtcaaaccga
      901 cacggatttt ttcggccatc gccaaggaga agatgatgtc taaaaaaatg gtaacgatcg
      961 acggaaacga gggcgtctgt cacgtcgcct acaaactgaa cgaagtcatc gccatttacc
     1021 ccatcacccc ttcttccggc ctgtccgaac gggccgacct gctggcctcc atgaaaaaac
     1081 ccaacctgtg gggggaagtc cccaccgtcg tggaaatgca gtccgaaggc ggcgcggccg
     1141 gggcggtcca cggggcgctt caaaccgggt ccctttcgac caccttcacc tcctcccagg
     1201 gccttctgct catgatcccg aacatgtaca agatcgccgg ggaactcacc tccaccgttt
     1261 tccacatcgc cgcccgctcc atcgcggccc agggcctttc cattttcaac gaccaccagg
     1321 acgtcatggc cgttcggcag accggttggg ccatcctgtc ctccaactcc gtgcaggaag
     1381 gcatggacat ggcgaccatc gcccaggcca gcacgttgtt ggcccgggtg cccttcgtcc
     1441 atttcttcga cgggttccgc acctcccacg aaatccagaa ggtggagctg gtgtccgatg
     1501 aggtcctggg cgccatgatc gacacgaagc tcatcaacga gcaccgaagc cggggcctgt
     1561 cgcccgaaca cccgttcatc cgcggcaccg cccaaaaccc cgacgtttac ttccaggccc
     1621 gggaaacggt gaatccctat tacaagaaag tccccggcat cgtgcaggcc gaaatggaca
     1681 agtacgccaa actcaccggc cgccaataca aaatttacga ttactacggg catccggaag
     1741 ccgaccgggt cctggttctc atgggatcgg gctgcgaatc ggtgtcggaa gtggtggatt
     1801 acctaaacgc ccggggcgag aaggtcggca tgttgaaagt tcgcctcttc cggcctttct
     1861 cggcggagca cttcgtgaac gccctgccca agtccgtcaa acagatcgcg gttctggaca
     1921 agacgaaaga acccggctcc cagggcgaac ccctttatct ggacgtgtcc tccgccatc
//
LOCUS       DAOQLS010000410         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39856_length_1971_cov_3.188935, whole genome shotgun sequence.
ACCESSION   DAOQLS010000410 DAOQLS010000000
VERSION     DAOQLS010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39856_length_1971_cov_3.188935"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="tyrS"
                     /locus_tag="PK362_07795"
     CDS             complement(<1..529)
                     /gene="tyrS"
                     /locus_tag="PK362_07795"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNC74766.1"
                     /translation="MKNLERLLRGVAQVFSRADLEKKLSLSRPLRVKLGVDPTSPDLH
                     LGHTVALNKLRQFQDAGHTVVFIIGDYTARIGDPSGRSETRPPLSAEAVAKNAATYLD
                     QVYHVLDKNKTEVRRNSEWLEPLFANRDADPRRTILGLMGRNTVQQLTEREDFQKRLK
                     AGTPVSLLELLYPLLQ"
     gene            complement(526..1662)
                     /gene="thiO"
                     /locus_tag="PK362_07800"
     CDS             complement(526..1662)
                     /gene="thiO"
                     /locus_tag="PK362_07800"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNC74767.1"
                     /translation="MSQYFDVAVAGGGVIGCSVAFHLAQKGAKVAVVEKRTLGGQASS
                     VAAGMLAAQEEAAGPDAFFDVCLASRAQFDALVPEVRALSSVDPEWETAGIWRVAADE
                     DEIPGLLAKKKWQEARGLSVHWVSPEELKEIHPGLAPAPGALYCPTDGQINSARWVQA
                     LEEAGRRLGVRFLDHAESVQFVREGRRISGIRASADLIAADQVVVAAGAWTPFLLETL
                     GVSLPLEPVKGQLMVLNGVPRAFRGPVYATPGYVVPRADGRLIIGATAERVGFNVRPT
                     LAAQRFLADWVARWCPALGPMPVMEFQAGLRPGTPDGWPVVGRLWEFDNLYIAAGHFR
                     NGILLSPWTGRYMAEGLLDGRWDPRGDAFSPERFRRASEPARPA"
     gene            complement(1659..>1971)
                     /locus_tag="PK362_07805"
     CDS             complement(1659..>1971)
                     /locus_tag="PK362_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74768.1"
                     /translation="TNTVFVGDDRDTLSDTFEVADAHWVHGRPPAAEFAAYVQIRSRH
                     SPVGAVLTVTAEGLRVKAEGLRAVTPGQAAVFYDGDDVIGGGVIRRAALREPVAPGSP
                     L"
BASE COUNT          347 a          657 c          666 g          301 t
ORIGIN      
        1 cctgcaggag cggataaaga agttccaaca gggacaccgg ggtaccggct ttgaggcgct
       61 tctgaaaatc ctcccgttcg gtcaattgtt gaaccgtgtt gcgccccatc aggcccagga
      121 tcgttcggcg gggatcggcg tcccggttgg cgaacagcgg ctccagccac tcggaattgc
      181 ggcgaacctc ggttttgttt ttgtccagaa cgtgatagac ctggtccaga tacgtcgccg
      241 cgtttttcgc caccgcctcc gcggacaaag ggggccgggt ttcggaacgg cccgaagggt
      301 cgccgatccg ggccgtgtag tcgccgatga tgaacacgac ggtgtgcccg gcgtcctgaa
      361 attggcggag cttgttcagc gccacggtgt gacccaggtg gaggtccggg gaggtgggat
      421 cgacccccag tttcacccgc aggggtcggg acagggaaag tttcttttcg aggtcggccc
      481 gggaaaacac ctgggccacc ccccgaagca accgctcgag gtttttcacg cggggcgcgc
      541 cggctcggag gcccggcgga accgctcggg cgaaaaagcg tcgccccggg ggtcccaacg
      601 accgtccaag aggccctcgg ccatgtagcg gcccgtccag ggggagagaa ggatgccgtt
      661 tcgaaaatgg cccgcggcga tgtaaaggtt gtcgaattcc caaagacgtc ccactacggg
      721 ccatccatcg ggggttccgg ggcgaagacc ggcctggaat tccatcacgg gcatgggccc
      781 gagcgccggg caccaccggg ccacccaatc ggccaggaag cgctgggccg ccagcgtggg
      841 ccgcacgttg aaccccaccc gctccgcggt ggcgccgatg atcaatcgtc cgtccgcccg
      901 gggtaccacg tagccgggcg tggcgtagac cggcccccga aaggcccggg gcacgccgtt
      961 taaaaccatg agttgtcctt tgacgggttc caagggaagg gaaacgccca gagtttccag
     1021 gaggaaaggc gtccaggccc ccgccgccac caccacctgg tcggcggcga tcaaatccgc
     1081 ggaggcccgg atcccggaaa tccggcggcc ttcgcgcaca aattggacgg attccgcgtg
     1141 atccaggaat cgcaccccca agcggcggcc ggcttcttcc agggcttgca cccaacgggc
     1201 gctgttgatt tggccgtcgg tggggcaata gagcgcgccg ggggcggggg ccaggccggg
     1261 atggatttcc ttcaattctt cgggggacac ccaatgcacc gacaaccccc gggcttcctg
     1321 ccatttcttt ttggccaaca ggccggggat ttcgtcctcg tcggcggcga cccgccaaat
     1381 cccggccgtc tcccattcgg gatcgacgga ggacagggcg cggacctccg gaaccagggc
     1441 gtcgaactgg gcgcggctcg cgaggcacac gtcaaaaaag gcgtcggggc cggcggcctc
     1501 ttcctgggcc gcgagcatgc ccgcggccac gctggaggcc tgccccccca aggtgcgttt
     1561 ttcgacgacg gccaccttcg cgcctttttg agccaagtgg aaggccaccg aacaaccgat
     1621 cacgccgccg cccgcgacgg cgacgtcgaa atactggctc aaaggggcga ccccggggcg
     1681 acgggctccc gcagggcggc gcgccggatc acgcccccgc cgatcacatc gtcgccgtcg
     1741 taaaacaccg cggcctggcc cggcgtcacc gcccgaagac cctcggcttt gacgcgaagg
     1801 ccctccgccg tgacggtcaa caccgcgccc acgggcgaat gacgggagcg gatttgaacg
     1861 taggcggcga actcggccgc cggggggcga ccgtgcaccc aatgggcgtc ggcgacctcg
     1921 aaggtgtcgc tcagcgtgtc ccggtcgtcg cccacgaata ccgtgttggt g
//
LOCUS       DAOQLS010000411         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_39936_length_1969_cov_3.334901, whole genome shotgun sequence.
ACCESSION   DAOQLS010000411 DAOQLS010000000
VERSION     DAOQLS010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39936_length_1969_cov_3.334901"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1969
                     /locus_tag="PK362_07810"
     CDS             <1..>1969
                     /locus_tag="PK362_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC74769.1"
                     /translation="ACVGAVSLRSLPIDAFPDVTSVQVEIVSTAEGKSPLEVEKLVTY
                     PVENVLRGLPGLTQLRSASKYGISVVTVVFKDGTDLYFARQQVSERLTEAREKLGDGI
                     EIAMGPPATAMGEIYQYTLDTGRETESPAQLAEMRTLQDWVVSPILKSVAGVGDINSF
                     GGYIKEYQVVALPDLMTQYNVSLKELYDAIGRNNSNVGGGLLESSGEQLLIRGLGLIK
                     SAAELGDIVVADRGGSPVLVRDIARVREGHAVRQGASFIDGKKETVGGVVMMLKGENS
                     RKVVGRVSKKVAEINASGVLPAGMKMKPFYTRDTVINSSVATIVKALAEGSAIVVVVL
                     YLFLLSFRGSFVTIMALPLASLLTFLAMRRFGISGNLMSLGGLAISIGMIIDSTIIQV
                     ENVLHHLGARREAEHRDKAVADAIIEVRKPSIFGELIIALTFIPMLALEGIEGKMFGP
                     LASTVMLALFSSLLLSLTVIPALCATVLRPAPDKESPLLTLAGRLYRPTLAWSMAHPR
                     KVLLAGGLLLAGCLALIPRLGTEFVPVMDEGSFDMDTGILPGASLETSAGAAKKIERI
                     LMKFPELETVVSKTGWTGRAIEARGVEKTGFLGVLKPRKEWKTAKTREELFEKMREAL
                     AVIPGLVIGFSQPIQCRIDELVAGTRSQLAVR"
BASE COUNT          376 a          549 c          667 g          377 t
ORIGIN      
        1 ggcttgcgtc ggggcggttt cgctgcgctc actccccatc gacgcctttc ccgatgtgac
       61 cagcgtgcag gtggagatcg tctcgacggc ggagggcaag tcccctctcg aagtggaaaa
      121 gctggtgacc tatccggtgg agaacgtttt gcgcggattg ccgggtctca cgcaattgcg
      181 ttccgcgtcc aaatacggca tctcggtggt caccgtcgtc tttaaagacg gcacggactt
      241 atacttcgcc cggcaacagg tttccgagag gctgacggag gcgcgcgaga agctgggaga
      301 cgggattgaa atcgccatgg gtccgcccgc gacggccatg ggcgaaatct accaatacac
      361 cctcgacacg ggccgggaga cggaatcgcc cgctcagctg gctgagatgc gcacattgca
      421 ggattgggtt gtttctccga tcctgaaaag cgtggcgggg gtgggcgaca tcaattcatt
      481 cggcggatac atcaaggaat accaagtggt ggctctgccg gacctgatga cgcaatataa
      541 cgtgagcctg aaagagctct acgacgcgat cggccgcaac aattccaacg tcggcggggg
      601 gttgctggaa tcatcagggg aacagctcct catacgaggg ttggggctga tcaaaagcgc
      661 ggcggagctg ggcgacatcg tggtggcgga tcgcggcgga tcgcccgtgt tggtgagaga
      721 catcgcccgc gtgcgggaag gccatgccgt gcgccagggg gcgtcgttca tcgacggaaa
      781 gaaagagacc gtgggcggcg tggtcatgat gctgaagggc gagaacagcc ggaaagtggt
      841 gggccgcgtt tcgaaaaaag tggctgaaat aaacgcgtcc ggggtgttgc ccgccggaat
      901 gaagatgaag ccgttctaca cgcgggacac cgtgatcaac agcagcgtgg ccaccatcgt
      961 caaggcgctg gcggagggtt ccgccatcgt ggtggtggtt ctctatctgt tcctgctcag
     1021 tttccgggga tcgttcgtca ccatcatggc gttgcccctg gcgtcgctct tgaccttcct
     1081 ggccatgcgg cgattcggga tttccggcaa cctcatgtcc ttgggcggcc tcgccatttc
     1141 catcggcatg atcatcgact ccaccatcat ccaggtggag aacgtcttac accacctcgg
     1201 cgcccgccgg gaggccgagc atcgggacaa agccgtggcc gatgccatca tcgaggtgag
     1261 gaaacccagc atcttcggcg agttgatcat cgcgctgacg ttcatcccca tgctcgcgct
     1321 ggagggcatc gagggaaaaa tgttcgggcc gctggcgtcg acggtgatgt tggcgttgtt
     1381 ctcttcgttg ttgctgtcgt tgaccgtcat accggccttg tgcgcgacgg tgttgcgccc
     1441 cgcgccggac aaggaaagcc cgcttttaac tctggctgga cgcctgtatc gcccgacgct
     1501 ggcatggagc atggcgcacc cgagaaaagt ccttttggcg ggagggctac tgctggccgg
     1561 gtgtctggcg ctgatcccgc gcctggggac ggagttcgtg ccggtgatgg acgaaggctc
     1621 gttcgacatg gacacgggca tattgcccgg ggcctccttg gagacctccg ccggagcggc
     1681 gaagaagatc gaacggatac tcatgaaatt cccggaactt gagacggtgg tttccaaaac
     1741 cggctggacc ggacgggcca tcgaggcgcg cggcgtggag aagacggggt ttctgggcgt
     1801 gctcaagccg cgcaaggaat ggaaaacggc gaaaacccgg gaagagcttt tcgaaaagat
     1861 gcgcgaggcg ttggccgtca tccccggcct ggtgatcggc ttcagccagc ccatccaatg
     1921 ccgcatcgac gagctggtgg ccgggacgcg gtcgcagttg gccgtgagg
//
LOCUS       DAOQLS010000412         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_40113_length_1965_cov_2.996335, whole genome shotgun sequence.
ACCESSION   DAOQLS010000412 DAOQLS010000000
VERSION     DAOQLS010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40113_length_1965_cov_2.996335"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /gene="ndhC"
                     /locus_tag="PK362_07815"
     CDS             complement(<1..274)
                     /gene="ndhC"
                     /locus_tag="PK362_07815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNC74770.1"
                     /translation="MTFQYLQVLLFAIIGFTFFSVLLLAAGLVRERGTTKDTSAYECG
                     MEPVGSAWISPNIRFYVFALLFVVFDVEALFVFPWAVQFRALGVEGF"
     gene            complement(271..954)
                     /locus_tag="PK362_07820"
     CDS             complement(271..954)
                     /locus_tag="PK362_07820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC74771.1"
                     /translation="MDVFTPGDIGRLFSPLAPRYRRFNRWASLGQDNRWRRSLIAEIE
                     GRRRVLDVGTGTGELAALAAARGASAVGLDVSPGMLEEARRRWPSGAWVLGDGKTFPF
                     PAGAFDAVVSAYVLRNLLKGGVLPGVLREARRVLSPDGRLVFLDLTRPEGGFQRWGHG
                     LYNRTILPALGRWFFGEHWPGDYLADSIDALPPADELRTIFQTCGFRSLTVRPLWGGV
                     VSLFIGSDV"
     gene            complement(994..1173)
                     /locus_tag="PK362_07825"
     CDS             complement(994..1173)
                     /locus_tag="PK362_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74772.1"
                     /translation="MAEMLVVVSKIKKMVKEKGFRTGGDYVDALSKKVEGIVNDSVTK
                     VQNEGKKKTLGAEDL"
     gene            complement(1378..>1965)
                     /locus_tag="PK362_07830"
     CDS             complement(1378..>1965)
                     /locus_tag="PK362_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNC74773.1"
                     /translation="DTVDVSNLHRQPLHGTPDRGRPKTVSAREKLTALNPAVRVVEHA
                     LRLTVDNAAAVFGGYDLVLDGSDNFETRYTVNDAAQRRGVPLVWGAVLRWEGQIMTVR
                     PGRSACYRCLFPEPPDPAEARSCADAGVAGPLAGLVGSWMALEALKALWGAGTALVNR
                     LLLIDGRAGRVRERVVERRPRCPACAPGAGVSTAV"
BASE COUNT          392 a          646 c          607 g          320 t
ORIGIN      
        1 caaatccttc gacgccgagg gctcgaaact ggaccgccca gggaaaaacg aacagggctt
       61 cgacgtcgaa gacgacgaaa agcaaggcaa aaacgtaaaa acggatgttc ggtgaaatcc
      121 aagccgatcc cacgggttcc atgccgcact cgtaggccga ggtgtctttg gtggtgccgc
      181 gctcgcgcac caaccccgcc gccaggagaa ggacggagaa gaaagtgaag ccgatgatcg
      241 cgaacagcag aacttgaagg tattgaaacg tcacacgtca ctcccaatga acaacgacac
      301 gacgcccccc cacagggggc ggacggtcag cgaccgaaaa ccgcaggttt ggaaaatcgt
      361 tcgaagttcg tccgcggggg gcaaagcgtc gatcgaatcc gccaagtaat ccccgggcca
      421 atgttcgccg aaaaaccaac gacccaacgc cggcaggatg gttcgattat acaaaccatg
      481 gccccaccgt tgaaagcccc cttcgggcct cgttaaatcc aaaaagacca aacgcccgtc
      541 cggggacagg acgcgtcggg cttcccgaag gacgcccggg agaaccccgc ctttgagcag
      601 gttccgcaaa acgtaggccg aaaccacggc gtcaaaagcg cccgccggaa agggaaaggt
      661 tttgccgtcc ccgagaaccc aggcgccgct tggccaccgc cgccgggcct cttccagcat
      721 gcccgggctg acgtccaagc ccaccgcgga ggcgccccgc gcggcggcca gcgccgccag
      781 ctccccggtg cccgtgccga cgtccaacac ccgccgtcgg ccttctattt ccgcgatcaa
      841 cgatcgccgc cagcgattgt cctgcccgag gctggcccat cggttgaatc gtcgataacg
      901 gggcgccagg ggagaaaaaa ggcgcccgat gtcccccggg gtgaagacgt ccatgaggac
      961 caccgcgggc cgtcggcccg ccggtgaagg ggtttaaagg tcttcggcgc cgagggtttt
     1021 ctttttgccc tcgttttgca ccttcgtcac ggaatcgttg acgatgcctt cgactttttt
     1081 ggacagggcg tccacgtaat cgccaccggt gcggaagcct ttttctttga ccatcttttt
     1141 gatcttgctg accacgacga gcatttcagc catgattacc tctctttttt cggtggcggg
     1201 gggttcccgc aaatcaagcg cggcgcgcgg cgctgggagc cgccggggcc agggcgttct
     1261 tccggtgttt cgtatttatt tcgggcgacc aacgggtttc cgatgtcgct ctcccgcgga
     1321 acgttgccct gcgccgttga agaagcgtgc ccattaaaga aatttgcccg gggaatgtca
     1381 aacggccgtc gagacgccgg cccccggggc gcaggccggg cagcggggac gccgctccac
     1441 cacgcgttcc cggacgcggc cggcgcggcc gtcaatgagc aaaagccggt tcaccagggc
     1501 cgtccccgcc ccccacagag ccttgagcgc ttccaaggcc atccagcttc ccacgagccc
     1561 cgccaggggg cccgcaacgc ccgcgtccgc gcaggaacgg gcctcggcgg ggtccggcgg
     1621 ttccggaaaa aggcaccggt agcaggccga ccgcccgggc cgcaccgtca tgatttgtcc
     1681 ttcccaccga aggacggctc cccacaccaa cggaacgccc cggcgttggg cggcgtcgtt
     1741 caccgtgtag cgggtttcaa aattgtcgga cccgtccagc accaggtcgt agccgccgaa
     1801 aaccgccgcc gcgttgtcga cggtcaagcg cagggcgtgt tccacgaccc gaaccgcggg
     1861 gttgagggcg gttaattttt cccgggcgga aaccgttttg ggacgtcccc ggtccggggt
     1921 tccgtgcaat ggttggcggt gaaggttgga gacatcgaca gtgtc
//
LOCUS       DAOQLS010000413         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_40463_length_1956_cov_4.647028, whole genome shotgun sequence.
ACCESSION   DAOQLS010000413 DAOQLS010000000
VERSION     DAOQLS010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40463_length_1956_cov_4.647028"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1104
                     /locus_tag="PK362_07835"
     CDS             124..1104
                     /locus_tag="PK362_07835"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HNC74774.1"
                     /translation="MLGIARRAVEDVTNAGQADVRSLNLAVRAVYAAESGTYLHAFGQ
                     DADGARDADLKREFLASLNQVFQLMGKPAPPEIRNGFARGGVPTAAEGEGSFEREGAT
                     LRWRDPLHDDRGPGNYFYPTGSQYETGTWDLRVFDVTPTTDAVVLGFEFAALPNPLKA
                     PLGFSYPLIDVYIDINHSPGAGAQELLPGRPGLVESQDAWEYALSIDGWGARLSQFVL
                     GGAPRAVATLSGQKTGAARFAVTVPRRYLRGDPDGWGFAVAVMGRAPPGGPMAVGSDP
                     GAAQFGGADAADRPAPPYIDLLTPEGVSQRRTLGVYRQGQDPTLPFVRAE"
     gene            1121..>1956
                     /locus_tag="PK362_07840"
     CDS             1121..>1956
                     /locus_tag="PK362_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC74775.1"
                     /translation="MSLPSKHDRFLFGVLAATLALGLVWMSERQSEGRVSSFLPAAWG
                     PKKDKGLPLDPVPVDGSVLNLQDSFAKVAAQVKPAVVSITATHIEKVQAPQFYFGDPF
                     EDFFREFQGMPRRGPGPAPAPRSFERRQQGLGSGVIVDPRGYILTNEHVVRGADELTV
                     TLQVPEERKFPGKVIGADPRTDLAVVKIAPPKEPLTYAPLGDSDRVRVGDWAIAIGSP
                     FGLEQTLTVGVISAVRQTLNIEGVNYTHLLQTDAAINRGNSGGPLLNIRGEVIGINSA
                     IYA"
BASE COUNT          308 a          633 c          665 g          350 t
ORIGIN      
        1 accgctcggt cgtaggtcag caggccgttg gcttcggtct ccacgtcggt ggttcgttcg
       61 cccttgcccg cgccggattt cacgccgtgg atcggggaca acgaggaaaa ccgggcctgg
      121 gaattgctgg gcatcgcccg gcgggcggtg gaagacgtga ccaacgccgg ccaggcggac
      181 gtgcggagcc tgaatctcgc cgtgcgggcg gtctacgccg ccgaaagcgg aacgtatctc
      241 cacgccttcg ggcaggacgc cgacggcgcc cgggacgcgg atttgaaacg ggaatttctc
      301 gcttcgctca atcaggtgtt tcaattgatg gggaagcccg cgccgcccga aattcgaaac
      361 ggttttgccc ggggcggggt cccgaccgcc gcggagggcg aagggtcttt cgagcgggaa
      421 ggggcgacgc tccgttggcg ggaccccctc cacgacgacc gggggcccgg caattatttt
      481 tatccgacgg ggtcccagta cgagaccggg acctgggacc tccgcgtttt tgacgtgacg
      541 cccaccaccg acgccgtggt cctcggattt gaattcgcgg cgctccccaa tccgctgaag
      601 gcgcccctgg gattttccta ccccctgatc gacgtctaca tcgatatcaa ccattccccc
      661 ggggccgggg cccaggaatt gctccccggc cgcccgggcc tggtcgaatc ccaggacgcc
      721 tgggaatacg cgttgagcat cgacggctgg ggcgcgcgct tgtcccagtt cgtcctgggc
      781 ggcgcgccgc gggccgtggc gaccttgagc ggacaaaaaa ccggggccgc tcggttcgcc
      841 gtgacggtcc cccgtcgtta cctgcggggc gatcccgacg gctgggggtt tgccgtggcg
      901 gtgatgggcc gggcgccgcc gggcgggccc atggcggtgg gctcggatcc gggcgccgcc
      961 caattcggcg gggcggacgc ggccgaccgg cccgcgcctc cctacatcga tttgttgacc
     1021 ccggaagggg tcagccaacg ccgcacgttg ggggtttacc gtcaagggca ggacccgacg
     1081 ttgcctttcg ttcgggcgga ataatttcaa ggagccttcc atgagcttgc cttccaaaca
     1141 cgatcgattc ctcttcgggg tgttggccgc gaccctggcc ctggggttgg tgtggatgtc
     1201 cgagcgccag agcgaagggc gtgtttcgtc ctttctgccc gccgcctggg gtccgaagaa
     1261 agacaagggg ctccccctgg acccggtgcc cgtggacggg tccgtgttga accttcagga
     1321 ttcctttgcc aaggtggccg cccaggtgaa gcccgccgtg gtcagcatca ccgccacgca
     1381 cattgaaaaa gtccaagccc cccaatttta tttcggcgat cccttcgagg atttctttcg
     1441 ggagttccag ggcatgcccc gccgggggcc cggtccggcc ccggcgccgc gttctttcga
     1501 gcggcggcag caggggctgg ggtcgggcgt gatcgtcgat ccccgcggct acatcctgac
     1561 caacgagcac gtggtccggg gcgccgatga attgaccgtc acgcttcaag tccccgagga
     1621 aaggaaattc ccgggcaagg tgatcggggc ggacccccgc acggatttgg cggtggtcaa
     1681 gatcgctccg cccaaggaac cgttgaccta cgccccgttg ggggactcgg accgcgtccg
     1741 ggtgggggat tgggccatcg ccatcggcag ccccttcggg ttggagcaaa ccctcaccgt
     1801 gggcgtgatc tcggcggttc ggcaaaccct gaacattgaa ggggtgaatt acacgcacct
     1861 gcttcaaacc gacgccgcca tcaatcgcgg aaattccggc gggccgctcc tcaatattcg
     1921 gggggaagtg atcggcatca attcggccat ctacgc
//
LOCUS       DAOQLS010000414         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_40644_length_1951_cov_4.493143, whole genome shotgun sequence.
ACCESSION   DAOQLS010000414 DAOQLS010000000
VERSION     DAOQLS010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40644_length_1951_cov_4.493143"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..80
                     /locus_tag="PK362_07845"
     CDS             <1..80
                     /locus_tag="PK362_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74776.1"
                     /translation="EKNVRFAIREGGHTVGAGVVTEILQ"
     gene            171..1331
                     /locus_tag="PK362_07850"
     CDS             171..1331
                     /locus_tag="PK362_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74777.1"
                     /translation="MNHRRALVPWGVGLAVVLGSVHGMAKEKKTAKGDRLGKDVTSVV
                     VTRYEKLSDTRDHPEKKKVAVIRKAETVGRLVTLINSLPPAGSAMIKMGDVDYIEAQF
                     YGHYDKPPVVVKLYHGWVKAPDTSFYEGLPAEKEIHEILSQAKPQPEGGLRVKKAKSV
                     RVEEYDHGFYANRDEGPNRSVEITDAKKVSALVDLLNQCPLKGGMFVSFTPYVSMTHV
                     FFVADNGDETQVTFYGNGLATTDGSFYTEEPGEGLQKRFRDIYMAELARTAPEQRYAF
                     DAAGALSASLELKKKLTDERADAREQVTDAAVLKKIQAALSKLPVRGEKMKSWSDDTE
                     LLTVTFSRPGAPKTYIEFYDGHLKAPDTAFYAGGNPEKALYQLLKTQLQKGK"
     gene            1336..1491
                     /gene="rpmG"
                     /locus_tag="PK362_07855"
     CDS             1336..1491
                     /gene="rpmG"
                     /locus_tag="PK362_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNC74778.1"
                     /translation="MANDRFVFMLACTECDNRNYHYVRGRRREKKLELKKFCQTCGKH
                     TPHKETK"
     gene            1512..>1951
                     /locus_tag="PK362_07860"
     CDS             1512..>1951
                     /locus_tag="PK362_07860"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNC74779.1"
                     /translation="MRKSGSFFAALLTAAAVSAAPVKYTGNKAVLQRIYDAMNSNDAT
                     ALEQVVSVNAVTHDLPAGMTPGVRGVEELLVVLNRAFPDFKIVVQDMVAEGDRVAARI
                     MFTGTHEGEFLGIARTGRQVAYTGIQYFQFANGKAVEMWSFMDH"
BASE COUNT          442 a          568 c          605 g          336 t
ORIGIN      
        1 tggagaagaa cgtgcggttc gcgatccgcg aaggcggcca cacggtgggc gccggagttg
       61 tgacggaaat attgcaatga aaaacggatt gatccggcgt cccatcctac aatctgggac
      121 ggcagcgaac gctgccgtcg gcaccgaaat tttggcgtaa ggggtttgtg ttgaaccacc
      181 gaagggccct tgttccctgg ggggtcggat tggcggtggt tctcgggagc gtccacggga
      241 tggccaagga aaagaaaacc gcgaaggggg accgtctcgg caaggacgtc acctccgtcg
      301 tggtgacgcg gtacgagaaa ctgtcggaca cacgcgacca tcccgagaag aagaaagtcg
      361 ccgtcattcg aaaagcggag accgtcgggc gcctcgtgac gttgatcaac tcgctgcccc
      421 ccgccgggtc ggccatgatc aagatgggcg acgtggatta catcgaagcc caattctacg
      481 gccactacga caagccgccg gtggttgtca aactttatca cggttgggtt aaggccccgg
      541 acacgtcgtt ttacgagggc ctcccggcgg aaaaagagat ccacgagatc ctttcccagg
      601 ccaagcccca gcccgagggc ggcctgcggg tgaaaaaggc caaatccgtc cgggtcgagg
      661 aatacgacca cgggttctac gccaaccgcg acgagggccc gaaccggtcg gtggaaatca
      721 ccgacgccaa gaaggtgtcg gccctggtcg acctgttgaa ccagtgcccg ctcaaggggg
      781 gcatgttcgt ttcgttcacg ccgtacgttt ccatgaccca cgtctttttc gtggcggaca
      841 acggcgacga gacccaggtg acgttttacg gaaacggcct ggccaccact gacgggtcct
      901 tttataccga ggagcccggc gaaggactcc agaagcggtt tcgggacatt tacatggcgg
      961 agttggcgcg gaccgcgccg gaacaacgct acgctttcga cgccgccggc gccctcagcg
     1021 cctctttgga actcaagaaa aagttgaccg acgaacgggc cgacgccaga gaacaagtca
     1081 cggacgccgc ggttttaaaa aagattcagg ccgcgctgtc caagctgccc gtccggggcg
     1141 agaaaatgaa gagttggtcc gacgacacgg agcttttgac cgtgaccttc agccggcccg
     1201 gcgcgccgaa gacgtacatc gaattttacg acgggcatct caaagcgccc gacacggcct
     1261 tttacgccgg ggggaacccg gaaaaggcgc tgtatcaact attgaaaaca cagctgcaaa
     1321 aaggaaagta aagtcatggc caacgatcgc ttcgtgttta tgcttgcttg caccgagtgc
     1381 gacaaccgca attaccacta cgtgcggggt cgtcgtcggg agaaaaagct ggaactgaaa
     1441 aagttctgcc aaacctgcgg caagcacacg ccccacaaag aaaccaaata accccttcgg
     1501 gaggacgccc catgcgcaaa tccgggagtt tcttcgccgc tcttttgacg gcggcggcgg
     1561 tttcggccgc gccggtcaaa tacaccggca acaaagccgt tctgcagagg atttacgacg
     1621 cgatgaattc caacgacgcc acggcgctgg agcaggtggt gtccgtgaac gccgtcaccc
     1681 acgacctgcc cgcgggcatg acccccggcg tgcgcggggt cgaggaattg ctcgtggtgc
     1741 tgaaccgggc ctttccggat ttcaaaatcg tcgtgcagga catggtcgcc gagggggacc
     1801 gggtggccgc gcggattatg ttcaccggaa cccacgaagg cgaattcctg ggcatcgccc
     1861 gcacgggccg ccaggtggcc tacacgggga ttcagtattt tcaattcgcc aacggcaagg
     1921 ccgtcgaaat gtggtccttc atggaccacg a
//
LOCUS       DAOQLS010000415         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_40720_length_1949_cov_4.760296, whole genome shotgun sequence.
ACCESSION   DAOQLS010000415 DAOQLS010000000
VERSION     DAOQLS010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40720_length_1949_cov_4.760296"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..101
                     /locus_tag="PK362_07865"
     tRNA            29..101
                     /locus_tag="PK362_07865"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62..64,aa:Ala,seq:cgc)
     gene            297..>1949
                     /locus_tag="PK362_07870"
     CDS             297..>1949
                     /locus_tag="PK362_07870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNC74780.1"
                     /translation="MSETTDNNAEFIKEFLIESHENLDKIVDQLLTLEKNPASPAPLA
                     EIFRAFHTIKGTGRFFNLAKIETLTHFGEDLLSRLRDGRLAFHRGCAEALLALSDGVR
                     TILKSLETTGQEGSLEVEALVKTLRPRAESPGGGALLREEAAAEAAPAVADTTLRVEV
                     QVLDRLMGQIGELVLARNHLLQRAAFSRDPAVAPAVQRLNFITADLQNQLMKMRLQPI
                     AGLWAKLPRLVRDLSVALGKEAQLFLEGGETELDKSVLESIKDPLTHLVRNAVDHGLE
                     APEKRRAAGKPVAGEIRLRAFHEGGVVHMEIADDGAGLSLDAIRRKALEKNLISPASA
                     ERLTDADVARLVFLPGLSTAPAVTNLSGRGVGLDVVKTNVEKIGGSVEVRSAPGRGCS
                     FHLKIPLTLAILPALVVSSRGLRYAVPQSGLLELVRLRPGVAGQDVEWVQGAAVFRRR
                     GRLLPVVFLNKILHGSDNADPAMALLVLQIGPRRFGLAVEHIVDTEEIVVKPLGRHLK
                     SLNLFEGATVLGDGAVVLILDIPGLADHGGVAATELAAPAFGD"
BASE COUNT          358 a          595 c          611 g          385 t
ORIGIN      
        1 ggcggcccag agggccgacc cctccactgg ggctgtagct cagctgggag agcgcgtcgt
       61 tcgcaacgac gaggtcgagg gttcgatccc cttcagctcc acaatttaaa accaacccga
      121 tcccgaaagg gatcgggttt tcttttttcg aataagaagc gttttcgctg ctccggaacc
      181 cgaccggagg gtcgtatttt tccttccttt atatgcggta cgccttgtct tgacaaaccc
      241 ccggaccccc cctagacttg attgaaggat cgacggcaca tccggggtga ctcacggtga
      301 gcgaaacgac ggacaacaac gcggaattca tcaaggaatt cctcatcgaa agccatgaaa
      361 acctggataa aatcgttgac cagctgttga ccctggaaaa aaatcccgct tcccccgccc
      421 cgttggccga aatcttccgg gcgttccaca ccatcaaagg cacgggccgt tttttcaacc
      481 ttgccaaaat cgagaccttg acccatttcg gggaagacct cctcagccgt ctgcgggacg
      541 gtcggctcgc ctttcatcgg ggatgcgccg aggctctgtt ggccctttcg gacggtgtcc
      601 ggacgatttt gaagagcctc gaaaccaccg gccaggaagg ttccctggaa gtggaggcgc
      661 tggtgaagac ccttcggccc cgggcggaat cccccggggg cggcgccctc ctccgggaag
      721 aagcggcggc cgaggccgcg ccggccgtgg ccgacacgac ccttcgggtg gaagttcagg
      781 tgttggaccg gctcatgggg cagatcgggg aattggtcct ggctcgaaat catctgttgc
      841 aacgggccgc cttttcccgg gacccggccg tggctccggc cgtccagcgc cttaatttca
      901 tcaccgccga tctgcaaaac cagctgatga aaatgcgcct tcaacccatc gccgggttgt
      961 gggcgaaatt gccccggctg gtccgggatt tgtcggtcgc cttgggaaaa gaggcccaac
     1021 tctttttgga gggcggcgaa acggaattgg acaagtccgt cctggaatcc ataaaagacc
     1081 cgttgaccca tctggtgcgg aacgccgtcg accacggcct ggaggcgccc gagaaacgcc
     1141 gggccgccgg aaagccggtc gcgggggaaa tccgcctgcg ggcctttcac gaaggcggcg
     1201 tggtgcacat ggagatcgcg gacgacggcg ccggcctttc cctggacgcg attcgacgaa
     1261 aggccctgga gaaaaatctg atctcccccg cttccgcgga acgactgacc gacgccgacg
     1321 tggcccggtt ggtttttctt cccggactct ccaccgcccc cgccgtgacc aatctgtccg
     1381 ggcggggcgt cgggttggac gtggtgaaaa cgaacgtgga gaagatcggc ggcagcgtcg
     1441 aggtccgctc cgccccggga cgcggctgtt ccttccattt gaagattccc ctgaccctgg
     1501 ccattttacc ggccctggtg gtctcctccc gggggcttcg ctacgcggtg ccccaatcgg
     1561 gattgttgga attggttcga ttgcggccgg gcgtcgccgg acaagacgtc gaatgggtcc
     1621 agggcgcggc ggtgttccgc cgacggggac gcctattgcc cgtggtgttt ttgaacaaaa
     1681 tattgcacgg gtccgataac gccgatcccg cgatggccct tctggttttg cagatcggac
     1741 cgcgtcgatt tggcttggcg gtggaacaca tcgtggacac cgaggaaatc gttgttaaac
     1801 ccctgggacg ccatctgaaa tcattgaatt tgttcgaggg ggccacggtg ctgggcgacg
     1861 gcgcggtggt gcttattctg gacattccgg gattggccga tcacggcggt gtggccgcca
     1921 ccgagctggc cgcgcccgcc ttcggcgac
//
LOCUS       DAOQLS010000416         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41248_length_1935_cov_3.613830, whole genome shotgun sequence.
ACCESSION   DAOQLS010000416 DAOQLS010000000
VERSION     DAOQLS010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41248_length_1935_cov_3.613830"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PK362_07875"
     CDS             <1..755
                     /locus_tag="PK362_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNC74781.1"
                     /translation="GAVAENRDRALARMAEVPADVYVLPELAFTGYHFDRPEDMAALA
                     EPAADGPSGRAMANFCQKKKAWAVFGFAESSGGRVFNAAALWGPTGLVGVYRKIHLFG
                     REKTLFSPGDGPFPVWSLPPGRMGLMICFDWFFPESMRALALAGAQIVLHPANLVLPH
                     CPQAMITRCLENRVFAATADRVGEETGGTGSLRFIGQSQIVSPRGDLLHRLGAEEGEA
                     VVDVRPAEADDKRLSSGNDLFAERRPEFYPRG"
     gene            complement(787..1587)
                     /locus_tag="PK362_07880"
     CDS             complement(787..1587)
                     /locus_tag="PK362_07880"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNC74782.1"
                     /translation="MKCKYMAFAFVLFGGRAAAGEEWHTQVDLVTVATTAAPGQTLWV
                     GVRLRTADGWHVFWANPGEVGLTTKVQWLSPPDAATAPIRWPWPLRILSDGWVAHTLP
                     EDATLWTPLRLADAVPMGAGWTVRARVEWLECRESCQAGSAEPSLFVVTDPRAPQADP
                     PVPPEEKAEPCRVRRVGKKNWELIFAPERGGQSNVPVVFFPRSLGWAASAPATPRVSD
                     EFRVLSIGRPPGALPKRLQGLLLGPAGWRGPGRSRGAWVDVPMPETYD"
     gene            1662..>1935
                     /locus_tag="PK362_07885"
     CDS             1662..>1935
                     /locus_tag="PK362_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74783.1"
                     /translation="MKTRRRPRRTFPNRSARRAGSALLSLLLAGNPLAAAMVEGRRFW
                     DDRRAATARSGAAPSDFTALLTQWPGEKPLWGGNAAPDSATAVAGAG"
BASE COUNT          308 a          647 c          655 g          325 t
ORIGIN      
        1 ggggggcggt ggcggagaac cgcgaccggg ccctggcccg gatggccgag gtgccggccg
       61 atgtctatgt gctcccggaa ctggcgttta ccgggtatca tttcgaccgc cccgaggaca
      121 tggcggccct ggccgagccg gccgccgacg gcccttccgg ccgggcgatg gcgaatttct
      181 gccaaaagaa aaaagcctgg gccgttttcg gattcgccga atcgtcgggg gggcgggttt
      241 ttaacgccgc cgccctttgg gggccgaccg gactggtcgg ggtttaccgc aaaatccacc
      301 tgttcggccg tgaaaaaacg ctcttttccc cgggggacgg gcccttcccc gtctggtccc
      361 tgccgccggg gcgtatgggt ctcatgatct gtttcgactg gtttttcccc gagtccatgc
      421 gcgccctggc cctggcgggg gcgcaaatcg ttctgcatcc cgccaatctg gtattgcccc
      481 attgccccca agccatgatc acccggtgtc tggagaaccg cgtgtttgcg gccacggccg
      541 atcgggtggg cgaagaaacc ggcgggacgg gttcccttcg ttttatcggt cagagccaga
      601 tcgtgtctcc ccggggagac ctgttgcacc gtttgggggc cgaggaaggc gaggccgtgg
      661 tggacgtccg tccggccgaa gccgacgaca aacggttgtc ctcggggaac gacttgtttg
      721 cggagcgtcg gccggagttt tatccccggg gatgaggggg cgaagggcga aaaacgaggg
      781 cgccgatcaa tcgtaagttt cgggcatggg gacgtccacc caggcgcccc gggaacggcc
      841 gggtccccgc cagcccgcgg gtcccagcag caggccctga agccgtttgg gaagggcgcc
      901 cggcggccgg ccgatcgaaa ggacccggaa ttcgtccgac acccggggtg tcgcgggggc
      961 cgaggccgcc cacccgaggg accggggaaa aaaaaccacc ggcacgttgc tttgtccgcc
     1021 gcgctccggg gcgaagatca attcccaatt ctttttcccg acccggcgaa cgcggcaggg
     1081 ctccgccttt tcctccggcg ggaccggggg atcggcctgc ggcgcacggg ggtcggtgac
     1141 cacgaacagc gacggttccg cgctgcccgc ttggcagctt tcccggcatt ccagccattc
     1201 cacccgggcg cggaccgtcc accccgctcc catgggaacg gcgtccgcca gccgcaacgg
     1261 cgtccaaagg gtcgcgtcct cggggagggt gtgcgccacc cacccgtcgg aaagaattcg
     1321 aaggggccag ggccagcgga tcggggcggt ggcggcgtcc ggcggcgaga gccattgcac
     1381 cttggtcgtc agtcccacct ccccggggtt ggcccaaaag acgtgccaac cgtcggccgt
     1441 gcgcagccgt acgcccaccc acagcgtttg tcccggggcc gcggtggtgg ccaccgtgac
     1501 caggtcgact tgggtgtgcc attcctcccc ggccgccgcg cggccgccaa acagcacaaa
     1561 cgcaaaagcc atgtatttac atttcattta caatccttta aggtccccgc ccgtgttcat
     1621 ctattaaact taatttgaga acgcgaaagc gtttcatcga catgaaaaca cgccgccgac
     1681 cccgacgaac tttcccgaac cgttccgccc gacgggcggg gagcgcgctg ttgtccctgc
     1741 tcctggccgg aaaccctttg gccgccgcga tggtggaggg acggcgtttc tgggacgatc
     1801 gtcgggccgc cacggcccgg tccggcgcgg ccccttccga tttcaccgcg ttgttgaccc
     1861 aatggcccgg cgaaaaaccc ctgtggggcg gaaacgccgc cccggactcg gcaacggcgg
     1921 tcgccggtgc cgggg
//
LOCUS       DAOQLS010000417         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41252_length_1935_cov_3.262766, whole genome shotgun sequence.
ACCESSION   DAOQLS010000417 DAOQLS010000000
VERSION     DAOQLS010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41252_length_1935_cov_3.262766"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PK362_07890"
     CDS             <1..775
                     /locus_tag="PK362_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015108702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC74784.1"
                     /translation="NIAFGLAVRGTPGEEIRERVRELLKLVQLEGYENRFPSQLSGGQ
                     RQRVALARALAPRPRVLLLDEPFGALDAKVRDELRGWIRRLHEEAHVTTLFVTHDQQE
                     AMEISGKILVMAQGRIEQEGTPFEIFDHPATQFVAQFVGETNHVDSVISQPELALWGP
                     FRFTVAGPAVGAQVRIYFRPNDVYLSSVPETLQVEGTIVKTRFRGPLIEHSVDVGADR
                     PIVAHVPKGVSLASGFDVGRRVYVGITAYHSFAVPAGAA"
     gene            772..1437
                     /gene="cysC"
                     /locus_tag="PK362_07895"
     CDS             772..1437
                     /gene="cysC"
                     /locus_tag="PK362_07895"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNC74785.1"
                     /translation="MTGGARVHKNLTPHAGRLSADRRREKLGQRGAVVWFTGLSGSGK
                     STVAREVEALLVEDGRNAAVLDGDNVRLGLNKDAALLSAAGYGEAAAGRFGLGFSAED
                     RTENIRRVGEVAKLFADNNVIALTAFISPFRADRDLVRALMPKGAFFEVHVAAPLAVC
                     EARDPKGLYKKARAGAVGEFTGIASPYEPPRAPELVLHSDRETPAESAQRVVALLKRK
                     GVL"
     gene            1502..>1935
                     /locus_tag="PK362_07900"
     CDS             1502..>1935
                     /locus_tag="PK362_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNC74786.1"
                     /translation="MTTIAPALLEELKDLADPVALLRDLHRRFGNRLAVGTSGQLSGS
                     AIVALCVEAGFTPRVFTNDTGRLFPETHDLFRRLEERYRLTVERFAPDPKILGEMVST
                     YGEYLFFDSKERQELCCEVRKVLPNDRALATLDVWVTGLRAD"
BASE COUNT          324 a          656 c          621 g          334 t
ORIGIN      
        1 caacatcgcc ttcggactgg ccgtccgggg aaccccgggg gaggaaatcc gggaacgggt
       61 tcgggaattg ttgaagctcg tccagctgga gggttacgag aaccggtttc cctcccagct
      121 ctcgggcgga cagcgccagc gggtggccct ggcccgggcc ctggcgcccc gcccccgggt
      181 gctcctcctg gacgaaccct tcggcgccct ggacgccaag gtgcgggacg aattgcgcgg
      241 ctggattcga cgcctgcacg aggaggccca cgtcaccacc ttatttgtca cccacgacca
      301 gcaggaagcc atggaaatct ccggaaagat cctggtcatg gcccagggtc gaatcgagca
      361 ggaaggcacg ccctttgaaa tcttcgacca ccccgccacc caattcgtcg cccagtttgt
      421 cggcgagaca aaccacgtgg actccgtcat cagccagccc gagttggccc tctggggacc
      481 gttccgattc acggtcgcgg gcccggcggt cggcgcccag gttcgaatct attttcgccc
      541 caacgacgtc tatttgagct ccgttccgga aacccttcag gtggagggca ccatcgtcaa
      601 aacgcgtttt cgcggtccgt tgatcgagca ctccgtggac gtgggggcgg accgacccat
      661 cgtggcccac gttccaaagg gcgtttccct ggccagcggc ttcgacgtgg gccgccgggt
      721 gtacgtgggc atcaccgcct accattcctt cgccgtgccg gcgggggccg cgtgaccggc
      781 ggcgcccggg ttcacaaaaa tctcaccccc cacgcggggc gtttgtcggc cgaccggcgg
      841 cgggaaaagt tgggccagcg gggcgccgtc gtgtggttca cggggctttc ggggtcgggc
      901 aaatccaccg tggcccggga agtggaggcg ctcctggtgg aggacggccg aaacgccgcc
      961 gtgctggacg gcgacaacgt gcgcctcggt ctgaacaagg acgccgcgct cctttcggcg
     1021 gccgggtacg gcgaggccgc ggccgggcgt ttcggtctcg gattctccgc cgaagaccgg
     1081 acggagaaca ttcgtcgcgt gggcgaggtg gccaagcttt tcgccgacaa caacgtcatt
     1141 gcgttaaccg ctttcatttc gcccttccgg gcggaccggg accttgtgcg agctttgatg
     1201 cccaaaggcg cttttttcga agtgcacgtg gcggcgcccc tggcggtctg cgaggcccgg
     1261 gatcccaagg gcctctacaa gaaggcccgg gccggcgcgg tgggggaatt caccgggatc
     1321 gcctccccct acgaacctcc ccgggcgccg gagctcgtgc ttcacagcga tcgcgaaacc
     1381 cccgcggaaa gcgcccaacg ggtcgtcgcc ctgttgaagc ggaagggcgt cctttaacgc
     1441 cgcggcgtct ccgcggcgac caaatggtaa aatcggcttc gcccccctcg gtgaaaaatc
     1501 catgacaacc atcgcccccg ccctgttgga ggaactcaag gacctcgcgg atcccgtcgc
     1561 cctgttgcgg gacctccacc ggcgcttcgg gaaccgcctg gccgtcggca ccagcggcca
     1621 attgagcggt tcggccatcg tggccctctg cgtcgaagcg ggcttcaccc cccgggtatt
     1681 caccaacgac accgggcgcc tctttccgga aacccacgac ctctttcggc ggctcgaaga
     1741 gcgataccgg ttgaccgtcg agcgtttcgc ccccgatccg aaaatcctgg gcgaaatggt
     1801 gtcgacctac ggcgaatatt tgtttttcga ctccaaggaa cggcaggaac tttgctgcga
     1861 agttcgaaaa gtgctgccca acgaccgcgc cctggccacc ctggacgtct gggtcaccgg
     1921 ccttcgggcc gacca
//
LOCUS       DAOQLS010000418         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41509_length_1928_cov_3.033102, whole genome shotgun sequence.
ACCESSION   DAOQLS010000418 DAOQLS010000000
VERSION     DAOQLS010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41509_length_1928_cov_3.033102"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..809
                     /locus_tag="PK362_07905"
     CDS             162..809
                     /locus_tag="PK362_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74787.1"
                     /translation="MRNLLVAAVLIGGFVWFLKSRGPSRAEDVSFAGQWKRAETSDVS
                     GSSTEELVQVKAAHGKFRLEKSVKSLGMTGAPLEYDVVLVFDGTTMHERETFKPRPGE
                     APRSPEESAPPTRSWKPSEGELREARFWARSFTGNAGPGGAVAGRETKLYQAKERRPD
                     AEATVQAWVDAKTGLVLRSNVSLFAKQVDSLLNKNTEECLSISFDPVPDSAFAKP"
     gene            813..1757
                     /locus_tag="PK362_07910"
     CDS             813..1757
                     /locus_tag="PK362_07910"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC74788.1"
                     /translation="MFIFLPLWPRRHTLPVATLALLLINALVFAVTWPLERAQTSVVS
                     ADHRAAAAQDLCRILKNEPSLAPADRDLLTAEEAPGFPTEGQDRLFRRIAENANQLSA
                     RGRYEWDSAYPIYASYSVSLKKNPRGGSVFQKWGFERGRFFPEIFTHQFLHAGFGHLF
                     FNMLFLWAVGGVVESAGSGLLVAVYLLGGVAAALGQLAWGLPDHQVMVGASGAISAIL
                     GFGLATVPRARTRVAYVAAPVLSVRYGTFNSPLWFFVPLWVFQQAVYGLVTRPYGGTT
                     VGYGAHLGGFAAGLAAGAAARFFRWWTPPVDPDGPGEF"
     gene            1757..>1928
                     /locus_tag="PK362_07915"
     CDS             1757..>1928
                     /locus_tag="PK362_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC74789.1"
                     /translation="MGIVRASVIDLRRLAVYRPAPLLAGVDWTLRRGEHWVILGANGS
                     GKSTLVNALAGYV"
BASE COUNT          299 a          604 c          636 g          389 t
ORIGIN      
        1 acggagcaat aggttgtagg ttcaaatcct actggggtcg gatttaatca acgccccgcc
       61 gttggtcccc tgggggacct ccggcggggg ttttattttc ctcggtcggt tgaatttcaa
      121 taaaataaac tcaattcttt tccgttgaac ggaggcgaat cgtgcgaaat ttgttggtgg
      181 cggcggtcct gatcggcggt tttgtgtggt ttcttaaatc ccgcggtccc tcccgggcgg
      241 aggacgtgtc cttcgccggt caatggaaac gggcggaaac gagcgacgtg tccggttcga
      301 gcaccgagga gctcgtccag gtgaaggccg cccacggcaa atttcgcctg gaaaaatcgg
      361 tgaagtccct cggaatgacg ggggcccccc tggaatacga cgtcgtcctg gtcttcgacg
      421 gcacgaccat gcacgaacgg gagaccttca agccccgccc cggggaggcc ccccgaagtc
      481 ccgaggaatc ggcgcccccc acccgctcct ggaaacccag cgagggggaa ctgcgggagg
      541 cgcgcttctg ggcccgttcc ttcaccggga acgccgggcc cggcggagcg gtggccgggc
      601 gggagacaaa gctttaccag gcgaaggaac ggcggcccga cgcggaggcg accgtccagg
      661 cctgggtgga cgccaagacc gggctcgttc ttcgttccaa cgtatcgctg ttcgccaaac
      721 aagtggattc cctgttgaac aaaaacaccg aggagtgcct ttcgatttct ttcgatccgg
      781 tccccgacag cgccttcgcc aaaccctgat tcgtgtttat tttccttccc ctctggccgc
      841 gccggcacac gttgccggtg gccacgctgg ccctgctcct gatcaacgcc ttggtttttg
      901 ccgtgacctg gcccctggag cgggcccaga cgtcggtggt gagcgccgat caccgggccg
      961 ccgcggccca ggacctttgc cgaatcctca aaaacgaacc gtccctggcc cccgccgacc
     1021 gcgatttgct cacggccgag gaggccccgg ggtttcccac ggagggccag gaccggcttt
     1081 tccgccggat cgcggagaac gcgaaccaat tgtcggcgcg gggccgctac gaatgggatt
     1141 cggcgtaccc gatttacgcc tcctacagcg tttccctcaa aaagaatccc agggggggga
     1201 gcgttttcca aaaatgggga tttgagcggg gtcgattttt ccccgaaatt ttcacgcacc
     1261 aattcctgca cgcggggttc gggcacttgt tcttcaacat gcttttcctt tgggccgtgg
     1321 ggggcgtggt ggaatccgcc gggtccggcc tgttggtggc cgtctaccta ttgggcggcg
     1381 tcgcggcggc cctggggcag ttggcctggg gactcccgga ccaccaagtc atggtgggcg
     1441 cctccggcgc gatttccgcg atcctggggt tcgggttggc gacggttccc cgggcccgaa
     1501 cgcgggtcgc ctacgtggcc gccccggtgc tatcggtacg ttacggcacc ttcaattcgc
     1561 ccctttggtt ttttgtgcct ttgtgggttt tccagcaagc ggtgtacggc ctggtcacgc
     1621 gaccctacgg cggaacaacc gtgggctacg gcgcccacct ggggggattc gccgcgggcc
     1681 tggccgccgg ggccgcggcc cggttctttc gttggtggac gccgcccgtg gacccggacg
     1741 gccccgggga attctgatgg gaatcgtccg ggcctccgtg atcgatttgc gccggttggc
     1801 ggtttaccgc cccgcgccgc tcctggccgg ggtggattgg accctgcggc ggggggaaca
     1861 ctgggtgatt ttgggcgcca acgggtcggg caaatccacc ctggtcaacg ccctggccgg
     1921 ttatgtca
//
LOCUS       DAOQLS010000419         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41632_length_1925_cov_3.689305, whole genome shotgun sequence.
ACCESSION   DAOQLS010000419 DAOQLS010000000
VERSION     DAOQLS010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41632_length_1925_cov_3.689305"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PK362_07920"
     CDS             complement(<1..1158)
                     /locus_tag="PK362_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC74790.1"
                     /translation="MGRRDAFRKITVRGPRALRVAAASLGGFALLSIAALPRPLFREP
                     TSTVLLGRDGGLLAAKIAADQQWRFPALDRVPAKFKIAAIQFEDRRFERHWGVDPFAV
                     ARALREAVRRRRVTSGASTLSMQVIRLARGNPPRTVPEKIWEMFLALGLELTHSKERV
                     LSLYAAHAPMGGNIVGLEAASWRYFGRAPESLSWAEAGVLAVLPNSPALMHPGRNRDR
                     LRAKRDRLLNRLHAAGFISDSDWPLALAEPLPDAPLPLPAHAVHLLDTLIQDRGLAGR
                     RIRSTLDPALQRAVEDLVAQRAESLRARDVQNVAAIVIDHRDFSVRAYVGNGGVSDGK
                     EGRSVDIVQCPRSTGSTLKPFLFAAALQAGDIGPTTLLPDLPTQFPGFHPEN"
     gene            complement(1128..1721)
                     /locus_tag="PK362_07925"
     CDS             complement(1128..1721)
                     /locus_tag="PK362_07925"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74791.1"
                     /translation="MGTGAPSSVTGEGRIFSKSLPDFPAGGAPVTVKNTGSRRLFAYV
                     TVRGVPRAGEETAASAGLELSVAYLKNGDPVDASSVKQGTDLTAQVTVRNRSGAKLDN
                     LALTHIVPSGWEIQSPAFEGGSTLYKGVEFRDVRDDRVNSYFSLAPGAVLTWEEQFHA
                     TYAGHFYLPAVAVEAMYDATKHARTTGQWVDVTRSEK"
BASE COUNT          339 a          612 c          667 g          307 t
ORIGIN      
        1 gttttccggg tggaacccgg ggaactgggt gggcaggtcc ggcaggagcg tggtcgggcc
       61 gatgtccccc gcctggagcg ccgccgcgaa cagaaagggt ttcagggtgc ttcccgtgct
      121 tcgggggcat tgaacaatgt cgacgctccg gccctctttt ccgtctgaaa ccccgccgtt
      181 ccccacgtag gcccgcacgg aaaaatcccg gtggtcgatg acgatcgcgg cgacgttttg
      241 aacgtcccgg gcgcgcagac tttccgcgcg ctgggcgacg agatcttcca cggcccgctg
      301 aagcgcgggg tccaaggtcg accggatccg tcggccggcc aagccccggt cctggatcaa
      361 cgtgtccagc agatggacag cgtgggcggg cagaggcagg ggcgcgtcgg gcaggggctc
      421 ggccagggcg aggggccaat cagagtcgga aataaatccg gcggcgtgca gccggttgag
      481 caatcgatcc cgtttggccc gaagacggtc ccggttccgg ccgggatgca tgagggcggg
      541 gctgttgggc aacaccgcca ggacgccggc ctccgcccag gaaaggcttt cgggcgcccg
      601 accgaaataa cgccaggacg cggcctccag ccccacgatg ttccccccca tgggagcgtg
      661 ggcggcgtag agcgacagaa cgcgttcctt ggagtgggta agctccagac ccagggcgag
      721 gaacatttcc caaatttttt cggggacggt ccgggggggg ttgccccggg ccaggcggat
      781 aacctgcatg gagagggtgc tggcgccgct cgtcacgcgg cggcggcgaa cggcttcccg
      841 cagggcccgg gccacggcga aggggtccac gccccagtgg cgttcaaacc gccggtcctc
      901 aaactggatg gcggcgattt tgaatttcgc ggggacgcgg tccaacgccg gaaagcgcca
      961 ttgctggtcg gcggcgattt tggccgccag caacccgccg tcgcgcccca acaggaccgt
     1021 ggacgtgggt tcgcggaaca acggccgggg cagggccgcg atggaaagga gggcgaatcc
     1081 gccgagggaa gcggccgcga cgcgaagcgc gcgcggcccc cggaccgtta tttttcggaa
     1141 cgcgtcacgt cgacccattg ccccgtcgtc cgggcgtgct tggtggcgtc gtacatcgcc
     1201 tccacggcga cggcgggcag gtagaaatgt ccggcgtagg tcgcgtgaaa ttgttcctcc
     1261 caggtcaaga cggctcccgg ggccaaggag aaataactgt tcacccgatc gtcccgcacg
     1321 tcccggaatt cgacgccttt gtaaagggtg ctgccgccct caaaagcggg gctttggatt
     1381 tcccaaccgg agggcacgat gtgggtcaaa gccaagttgt ccaacttggc gccggaacgg
     1441 ttccgaaccg tcacctgggc cgtcaggtcc gtgccctgct tgaccgagga tgcgtccacc
     1501 ggatccccgt tctttaaata cgcgacgctg agctcgaggc ccgcggacgc ggcggtttcc
     1561 tccccggccc tgggcacgcc ccgcaccgtc acgtaggcaa agaggcgtcg ggaccccgtg
     1621 ttcttgaccg tgaccggcgc gcccccggcg ggaaagtcgg gcagggactt ggagaagatg
     1681 cgtccttcgc ccgtgaccga cgacggcgcg ccggttccca ccaccccaag cgggcatctg
     1741 gtgaaggaaa cctaaatctc tatcggtata gtagacaaac gagcttccct tgaaaaggcc
     1801 acaagttttt cttatgccgg ccctggcgcc ggtgcagggt gaaatcgaaa ggcagcttgc
     1861 cggacctatg ctaaacatct ggcccggcgg gcgattctgc cgggcggcca gcgagagttc
     1921 ccacc
//
LOCUS       DAOQLS010000420         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41648_length_1925_cov_2.589305, whole genome shotgun sequence.
ACCESSION   DAOQLS010000420 DAOQLS010000000
VERSION     DAOQLS010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41648_length_1925_cov_2.589305"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1925)
                     /locus_tag="PK362_07930"
     CDS             complement(<1..>1925)
                     /locus_tag="PK362_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC74792.1"
                     /translation="VAYVGWEFYRGGVFHRWFNLNTDNAAPPPAHDPAFPGCPEGTER
                     VTAWLGGAWERGEACRETGGEKLLNGPFMAWHPENKKALEGAFAHGLKTGPWKQWDAA
                     GTMIVEGAYARDRRTGWWTFQLPPEAPFQKNYDVPLDCAALWGKKRWVVRPGACFGGI
                     GLNTAPGELPALFGEKEAMVQTLQPVGFLRPVSYANLFSESEEQTSVEWGPLGEPLNA
                     RVVSPKWTTVGGIRVGLTVADLEKLNGEPIPVSLREGQPVLESFGRGKLAEEGRCLEI
                     DLLKNRRESEEDSDETENRPVAAEEKKSLLVAGFTTYFSPEEKYRSESLRRAFSALDR
                     DAKRFQPLIDSRFFWLMEKGALSLMEGATGRPKWTVPLDEKSRAVTPVDVNGLGLFLR
                     ETSLVALDLESGKTKWAAATDRSAAFLLNDQNAPLVTDGNRVVLWRPAAGGARIKSVS
                     LADGRTAWERTLPAFLLQGTARNLLALTTTTVQSINAGTGALEWTAPLEAEGLRGRWM
                     SDGERWLAPVKDGLRCVSPQGENLWQCNLPVGTVPQTVVAAGPVDLLFCLTRSVTPED
                     AGRLIALNAGDGKKRWETSGSTNYLQRPRVAGDAVAFLDGEGLLRVLDIDSGRELWRT
                     EGKRKMDLGLPTAPPVF"
BASE COUNT          320 a          699 c          570 g          336 t
ORIGIN      
        1 aaaaacaccg gcggcgccgt cggcaggccc aaatccattt ttcgttttcc ctcggtccgc
       61 cagagttccc gaccgctgtc gatgtccagc acccgcagaa ggccctcccc gtccaggaaa
      121 gccaccgcgt ccccggccac tcgggggcgc tgcaggtagt tcgtggaccc ggaagtttcc
      181 caacgcttct tcccgtcccc ggcgtttagg gcgatcaacc ggcccgcgtc ctccggggtc
      241 acggagcggg tcaggcagaa gagcaggtcc acgggccccg cggccaccac cgtctgggga
      301 accgtcccca ccggcaggtt gcactgccaa aggttttcgc cctgggggct cacgcaccgg
      361 aggccgtcct tcacgggggc cagccagcgc tcgccgtcgc tcatccagcg accccgaagt
      421 ccctcggcct ccaggggcgc cgtccattcc agggcccccg tcccggcgtt gatcgattga
      481 accgtcgtgg tggtgagcgc caacaaattc cgcgccgtgc cctgaaggag gaacgcgggc
      541 agggttcgct cccaggccgt tcggccgtcg gccagggaca cgcttttgat ccgcgcgccg
      601 cccgccgccg gtcgccagag gacgacgcga ttcccgtcgg tcacgagcgg ggcgttttgg
      661 tcgttcagca aaaaggccgc gctccggtcc gtcgccgccg cccatttggt tttgccggat
      721 tccaggtcca gagccaccag cgaggtttcc cgcaggaaaa gccccagccc gttcacatcc
      781 accggcgtga ccgcccggga tttttcgtcc aggggaacgg tccatttcgg ccgccccgtg
      841 gccccttcca tcagggaaag agcgcccttt tccatcaacc aaaagaaccg cgaatcaatc
      901 aacggttgaa accgcttggc gtcccggtcc agagcgctga aggcccgccg cagggattcg
      961 cttcggtatt tttcctcggg ggaaaaatac gtggtgaaac ccgccaccaa aagggacttt
     1021 ttttcctccg ccgccaccgg gcggttttcc gtctcgtccg aatcctcctc cgattcccgg
     1081 cggtttttaa ggaggtcgat ctccaggcac cggccctcct cggccagttt gccccgccca
     1141 aaggattcca acacgggttg gccctcccgc agggaaacgg ggatcggctc gccgttcaac
     1201 ttttccaagt cggccaccgt cagccccacc cggattcctc cgaccgtcgt ccatttgggg
     1261 gaaacgacgc gggcgttcaa gggctctccc agggggcccc attccacgga ggtctgctcc
     1321 tcggattcgg aaaacaggtt ggcgtaggac acgggccgga gaaaccccac cggctgcaac
     1381 gtctgaacca tcgcctcctt ttctccaaaa agggccggca actcccccgg cgcggtgttc
     1441 aatccgatgc cgccgaagca ggcccccggc cgcaccaccc agcgcttctt gccccagagg
     1501 gcggcgcagt ccaggggaac gtcgtaattc ttttgaaagg gcgcctccgg gggcaactgg
     1561 aacgtccacc aacccgtccg ccggtcccgg gcgtaggcgc cctcgacgat catcgtcccc
     1621 gccgcgtccc attgcttcca cgggccggtc ttcaggccgt gggcgaaggc cccttccagg
     1681 gcctttttgt tttcggggtg ccaggccatg aagggcccgt tcaaaagttt ctctcccccg
     1741 gtttcccggc aagcttcgcc ccgttcccag gcgcccccca accaggcggt gacacgctcc
     1801 gtcccctcgg ggcatcccgg gaaagcggga tcgtgggccg gcggcggggc cgcgttgtcg
     1861 gtgtttaaat taaaccatcg gtgaaaaacc ccgccccggt aaaactccca cccgacgtag
     1921 gcgac
//
LOCUS       DAOQLS010000421         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41793_length_1921_cov_4.308146, whole genome shotgun sequence.
ACCESSION   DAOQLS010000421 DAOQLS010000000
VERSION     DAOQLS010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41793_length_1921_cov_4.308146"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK362_07935"
     CDS             <1..308
                     /locus_tag="PK362_07935"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570582.1"
                     /note="catalyzes the formation of fructose
                     1,6-bisphosphate from fructose 6-phosphate and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNC74793.1"
                     /translation="NGLTGYLSSVQNLTKPSAEWKAGGVPLTMMMNVERRKGKEKPVI
                     QKALVRLDDAPFAAFAKARESWAAADDYVFPGPIQYFGPAAVTDAPTRTLRLESARR"
     gene            346..774
                     /locus_tag="PK362_07940"
     CDS             346..774
                     /locus_tag="PK362_07940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC74794.1"
                     /translation="MIHEMEFRVAYADTDRMGVVYYANYLVLFERGRTELLRGLGLRY
                     RDFEETLKVFLPAVHAELNYLAPARYDDLLKIRTWVSDLGRASITFGYEVVNAESGRV
                     ITRGSTKHPFVDPRWKPVPVPAALRPALEKIVEPGAADPS"
     gene            771..>1921
                     /locus_tag="PK362_07945"
     CDS             771..>1921
                     /locus_tag="PK362_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC74795.1"
                     /translation="MNAVAVPLRRLSSNPVAAALVPAGVLFFLFMVTRIVLAVLARHE
                     LDATVLAWARTWWSGVRLDVVMALVFSLPWAILFSILPDRTRAGRWFPRALSVAWFAF
                     LFAFLFVAALEVAFFDEFDARFNSIAVDYLIYPTEVAGNIWQSYPVVRVVLAVGALAW
                     AIHRATARFFTAWAGRAAGGRKRGLGVGAMALLLAAAILLAPPVAAEISVNRVQNEIA
                     GNGPAAFFYAFWSNDLPYDHYYRTIPKDEAAQRVRRLLADSEAETVSGGVAGPLDRRE
                     WERGALDRPNFVVLIEESFGANFTGVLGRHPENLTPCFDRLAAGGILFTNFYATGSRT
                     VRGLEAILCGFPPVPGESIVKRHRSEKVYSFARALKDQGYETLFVYGGRG"
BASE COUNT          276 a          634 c          611 g          400 t
ORIGIN      
        1 tgaacgggtt gaccggttac ctctcctcgg tgcaaaacct gacgaagccc tccgccgaat
       61 ggaaagccgg cggggtgccc ctgaccatga tgatgaacgt cgagcgccgc aagggcaagg
      121 aaaagcccgt cattcaaaag gctttggtcc gattggacga cgcgcccttc gcggccttcg
      181 ccaaggcccg ggaatcctgg gccgcggccg atgattacgt tttccccggt cccattcaat
      241 acttcggtcc ggccgccgtg accgacgccc ccacccgaac ccttcggctc gagtcggcgc
      301 gccgctgagc gaccccgcca tgacgccccg ctcgggaggc cgccggtgat ccacgaaatg
      361 gaattccggg tggcctacgc cgacaccgac cgcatggggg tcgtctatta cgccaattat
      421 ctcgtgctct tcgaacgggg ccgcaccgag ctccttcgcg gcctggggtt gcggtaccgg
      481 gactttgagg agaccttgaa ggtgttcctt cccgcggtcc acgcggagct gaattacctg
      541 gcccccgccc gttacgacga tttgctcaag attcgaacgt gggtgtccga ccttggccgg
      601 gcgtccatca ccttcggata cgaagtggtg aacgcggaat ccggccgcgt catcacccgg
      661 ggcagcacca aacacccctt cgtcgatccc cgatggaagc cggtgccggt tcccgccgcc
      721 cttcgcccgg ctttggaaaa gatcgtcgaa ccgggagcgg ccgacccctc ttgaacgccg
      781 tcgccgttcc cctccgtcgc ctttcgtcga accccgtcgc cgccgccctg gtgcccgccg
      841 gggttttgtt ttttttattc atggtcaccc ggatcgtttt ggccgttctg gcccggcacg
      901 aattggacgc gacggtgctg gcctgggccc gcacctggtg gagcggcgtt cggctggacg
      961 tggtcatggc gctcgttttc tccttgccct gggcgatcct cttttccatc ctccccgatc
     1021 ggacccgcgc gggccgttgg tttccccggg cgttgtccgt ggcctggttc gccttcctgt
     1081 tcgccttttt gtttgtggcg gccctcgagg tggctttttt cgatgaattc gacgcgcgtt
     1141 tcaattccat cgcggtggac tatttgatct acccgacgga ggtggcggga aacatttggc
     1201 agtcctatcc cgtggtccgg gtggttctcg ccgtcggcgc cctggcgtgg gcgatccacc
     1261 gcgcgacggc gcgttttttc acggcttggg cgggacgggc cgcgggcggc cggaagcgcg
     1321 gtctcggcgt cggcgcgatg gccctgctcc tggccgccgc gatcctcctc gcgccgcccg
     1381 tcgccgcgga aatcagcgtc aaccgggttc aaaacgaaat cgccggaaac ggccccgccg
     1441 cttttttcta cgccttttgg agcaacgatc ttccgtacga tcattactac cggacgattc
     1501 ccaaagacga agccgcccaa cgggtccggc gcctgttggc ggattcggag gccgaaacgg
     1561 tgtcgggcgg agtggcgggt cccttggacc ggcgggagtg ggagcggggg gccttggacc
     1621 gtcccaattt cgtcgtgttg atcgaggaga gcttcggcgc caatttcacc ggggtgttgg
     1681 gccgacatcc cgaaaacctc actccctgtt tcgatcgcct cgccgcgggg ggaattctct
     1741 ttacgaattt ttacgccacc gggtcccgga cggtgcgcgg tctggaggcc attctgtgcg
     1801 ggttcccgcc ggtgccgggg gaatccatcg tcaagcgcca tcggtcggag aaggtctatt
     1861 ccttcgcccg ggccctgaag gatcaaggct acgagaccct gttcgtctac ggcgggcggg
     1921 g
//
LOCUS       DAOQLS010000422         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41826_length_1920_cov_4.907775, whole genome shotgun sequence.
ACCESSION   DAOQLS010000422 DAOQLS010000000
VERSION     DAOQLS010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41826_length_1920_cov_4.907775"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="murC"
                     /locus_tag="PK362_07950"
     CDS             <1..1176
                     /gene="murC"
                     /locus_tag="PK362_07950"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNC74796.1"
                     /translation="VLVTSTAVSPDNPEVLEARRRGIPIIPRIEMLAEIARLKHTIAV
                     AGSHGKTTTTSLAALVLQAGNLDPTVVIGGRLHSTGTGARVGKGKYLVAEADESDGSF
                     LKLSPTLAVITNIDDDHLDHWKTMDALDTGFVDFANKIPFYGAVFMCPDDPGVQRVRP
                     RIQRPVVTYGFSETADLRAEDVRFTPAQVSFRVRRGKDLLGLVRWACPGRHNILNALA
                     AIGVGLETGLLFGRIAESLSTFTGVGRRLELKGEAGGVTVVDDYGHHPTEIKATIEAL
                     RQRWPDRRVVALFQPHRYSRTKQLMEAFATAFTGVDQLFLLDIYPAGEKPLPGVSSDA
                     LADRLRAQSVLVERLLPTDDEHRLVPLIRANDVVLTLGAGDVWKWGEKLLPHLHRPA"
     gene            1173..>1920
                     /locus_tag="PK362_07955"
     CDS             1173..>1920
                     /locus_tag="PK362_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNC74797.1"
                     /translation="MTDGALPFLADLQRLFPNAKGREPLSRHTTWGIGGPADYYVEVE
                     NRDALDRLVTWAGDHGIPLWPIGQGSNLLVGDRGIRGIVTRLRGDFETFRFEDGARVN
                     AGAGVLLPQLSRACAERGLSGAEPFAGIPGTLGGGLMTNAGTPEGDLGSLVEEVLTLD
                     AEGRERLLTGKDLRFGYRRSNLIDHWVLSARLKLRPGDRNDILASVEKQRMRRAERQP
                     LGTKNCGSVFKNPPGDHAARLIEAAGLKGTR"
BASE COUNT          335 a          696 c          578 g          311 t
ORIGIN      
        1 gtcctggtca cgtccaccgc ggtcagcccc gacaaccccg aagtgctgga ggcccgccgc
       61 cggggcattc ccatcattcc ccgcattgaa atgctggcgg aaatcgcccg cctcaaacac
      121 accatcgccg ttgccgggtc ccacggcaaa accaccacca cgtccctcgc ggccctggtc
      181 cttcaagcgg ggaacctgga ccccacggtg gtcatcggcg gacggctcca ttccaccgga
      241 accggcgccc gggtgggcaa ggggaaatac ctcgtggccg aagccgacga atccgacggg
      301 tcctttttaa aactctcgcc caccctcgcg gtcatcacca acatcgacga cgatcacctg
      361 gaccattgga aaaccatgga cgccctggac acggggttcg tggattttgc caacaagatc
      421 cccttttacg gcgccgtgtt catgtgcccc gacgacccgg gcgttcagcg ggtccggccg
      481 cggattcagc gccccgtggt gacctacggt ttttccgaga cggccgacct tcgggccgaa
      541 gacgtccggt tcaccccggc ccaggtcagt tttcgcgttc gccggggaaa ggacctgctg
      601 ggccttgttc gctgggcctg ccccggccga cacaacattc tcaacgcctt ggccgccatc
      661 ggcgtcgggc tggaaacggg actgctcttc gggcgcatcg ccgagtccct gtccaccttc
      721 accggcgtgg gtcgccggct ggaactcaaa ggggaggcgg gcggcgtcac ggtcgtcgac
      781 gattacggac accaccccac ggaaatcaaa gcgaccatcg aagccctgcg gcaacgatgg
      841 ccggatcggc gggtcgtcgc gctctttcaa ccccaccgct acagccggac gaaacaattg
      901 atggaggctt tcgcaacggc cttcaccggc gtcgaccagc tttttctttt ggacatctac
      961 cccgcggggg aaaaacccct gcccggcgtc agcagcgacg ccctggccga ccgcctgcgg
     1021 gcccaatccg tgctcgtgga gcgtttgttg ccaaccgacg acgaacatcg cctcgtcccc
     1081 ctgatccgcg ccaacgacgt ggttttgacc ctcggggccg gagacgtttg gaaatggggg
     1141 gaaaagctgc tcccccacct ccaccgcccc gcgtgaccga cggcgccctc ccctttctcg
     1201 ccgacctcca acgccttttc ccgaacgcca agggccggga acccctgtcc cgccacacca
     1261 cctggggcat cggcggaccg gccgactatt acgtcgaggt ggagaaccgc gacgccctgg
     1321 accgcctcgt gacctgggcc ggcgaccacg gaatcccgct ctggcccatc ggccaagggt
     1381 ccaacttgct cgttggggac cgagggattc gggggatcgt cacgcgcctt cggggggatt
     1441 tcgaaacctt ccggttcgaa gacggcgcgc gggtcaacgc cggcgccggc gtgttgctcc
     1501 cccaattgtc ccgggcctgc gccgaacggg gtctgtccgg ggccgaaccc ttcgccggca
     1561 ttcccggcac cctggggggc ggcctcatga ccaacgcggg aacccccgaa ggcgatctgg
     1621 gctccctggt ggaagaagtc ttgaccctgg acgccgaggg ccgcgaacgc cttctcaccg
     1681 ggaaggacct ccgcttcggc taccgccgtt ccaacctgat cgaccactgg gtcctgtccg
     1741 cccgcctcaa attgcggccc ggggacagaa atgatatcct cgcttccgtc gagaaacaga
     1801 ggatgcgccg cgccgagcga caaccgctgg gcaccaaaaa ctgcggctcc gttttcaaaa
     1861 atcctcccgg ggaccacgcc gcccgcctca tcgaagcggc gggcctcaag ggaacgcggg
//
LOCUS       DAOQLS010000423         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41839_length_1920_cov_3.635925, whole genome shotgun sequence.
ACCESSION   DAOQLS010000423 DAOQLS010000000
VERSION     DAOQLS010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41839_length_1920_cov_3.635925"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK362_07960"
     CDS             complement(<1..273)
                     /locus_tag="PK362_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74798.1"
                     /translation="MAVLMAVGAGPLSAATPGRGAGQWRVEPQVVIGLGLKSHEVGVT
                     TDGQSVKISAGGGVGAGISLGYGLTESTDFEVLWGYQRSTLSPDVTN"
     gene            476..1702
                     /locus_tag="PK362_07965"
     CDS             476..1702
                     /locus_tag="PK362_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74799.1"
                     /translation="MFTGTTGAWKIVLSILFFPLFFQNHFVAFAWGAGEPWPLRLVKW
                     LFLLPGVLAFILACWVTMASLLTIPVRQNRSAFITGLLITWWDLGRSVFAFWGGAFRF
                     VFYLAAALFAVVKLIVIGTWAVLQDFILFPFRVVKNLGENVTKAGVPWIAVFLTLIWA
                     VLEAAIFTYVTTPVVIDTFSNMTGNELSEASIRIPLFIFMLFIVLGSYAVLSTWAEAV
                     KSKNVGAIIKIGVIELVTLFVEVVFLYREFVDSLVPWFAQHTSGDFDLGIVGILAISS
                     LTWLGVRGISWFLFASHGTPTLMAVLRGSGIHVPVTSNAQKEVSPTTMTASLWAAVKN
                     DLTFIQKKGDEVLAAFIIPPLQVLASAINFLTLLVTSEHLFRLPFKSIEEVMDSKVVL
                     HSVGQVRIDAHKPEAK"
     gene            1699..>1920
                     /locus_tag="PK362_07970"
     CDS             1699..>1920
                     /locus_tag="PK362_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74800.1"
                     /translation="MNRSSLRWKALVLAAALLPACGGTAPDVKKPRLSLFVGVDISRS
                     FLEGPNYEDSMDFLAHYLYAHMKGLGGLEV"
BASE COUNT          311 a          679 c          489 g          441 t
ORIGIN      
        1 gttcgtgacg tccggcgaca gcgtgctccg ctggtaaccc cacagcactt cgaaatccgt
       61 cgattccgtc aatccatacc ccaaactgat tcccgcgccc accccgcccc cggcgctgat
      121 tttcacgctt tggccatcgg tggtcacgcc cacctcgtgg ctcttcaggc ccagaccgat
      181 caccacctgg ggctccaccc gccattgccc cgcgccgcgc ccgggggtgg ccgccgacag
      241 gggcccggcc cccaccgcca tcaaaaccgc cattcctccc accctggaaa tcgttttcat
      301 gtcgtcctcc ttctatcgct gattcggggc gcccgtctat tgtctctttg gaattatagc
      361 aaccgaccgg aggtccggcg cgcccggccg atccccactt gcgcaacgcc gaatccggct
      421 tatactagca ccaagcccta aattgagtgg cctttcctta acccaaggag ctcccatgtt
      481 tactggaacc accggcgcct ggaaaattgt cctttccatt cttttcttcc ccctgttttt
      541 tcaaaaccat ttcgtcgcct ttgcctgggg ggcgggcgaa ccctggcccc tgcgcctggt
      601 caagtggctt tttctcctgc cgggcgtcct ggccttcatc ctggcttgct gggtgaccat
      661 ggccagcctg ctcaccattc cggtacggca gaaccgctcg gcgttcatca ccggcctgtt
      721 gatcacttgg tgggatttgg gccggagcgt cttcgccttc tggggcgggg ccttccgttt
      781 cgtcttttac ctggcggccg cgctcttcgc cgtcgttaaa ttaatcgtca tcggcacctg
      841 ggccgtgcta caagacttca tcctgttccc cttccgcgtg gtgaagaact tgggcgaaaa
      901 cgtcaccaaa gccggcgttc cctggatcgc cgtattcctc accttgattt gggcggtgct
      961 cgaggcggcg attttcacct acgtcaccac cccggtggtc atcgacacct tctccaacat
     1021 gacgggcaac gagctgtccg aagcctccat ccgcatcccc ctcttcatct tcatgctctt
     1081 catcgttctc ggcagttacg ccgtgctctc cacctgggcc gaggcggtca aaagcaaaaa
     1141 cgtcggggcc atcatcaaga tcggcgtcat cgagctcgtg accctcttcg tcgaagtcgt
     1201 ctttctctac cgggagttcg tggactctct cgttccctgg ttcgcccagc acacctcggg
     1261 cgacttcgac ctgggcatcg tcggcatcct ggccatttcc tccctgacct ggctgggcgt
     1321 ccgcggcatt tcctggttcc tcttcgcctc ccacggcacg ccgacgctca tggccgttct
     1381 ccgcggctcg ggcatccacg tgccggtgac ctccaacgcg caaaaggagg tgagccccac
     1441 caccatgacg gcctctctct gggccgcggt caaaaacgac ttgaccttca tccaaaagaa
     1501 gggggacgaa gtcctggccg cctttatcat cccgccgctc caggtgctgg cctcggccat
     1561 caactttctg acgctcctgg tgacgtccga acacctcttc cgcctgccgt tcaaatccat
     1621 tgaagaggtc atggattcca aagtcgtttt gcacagcgtc ggccaggtcc gcatcgacgc
     1681 ccataaaccg gaggccaaat gaaccgttca tccctgcgtt ggaaagccct ggttttggcg
     1741 gcggcccttc ttccggcctg cggcggcacc gcgcccgacg ttaagaaacc gcgtctttcc
     1801 cttttcgtcg gcgtcgacat cagccggtcc ttcctggaag gcccgaatta tgaagactcc
     1861 atggacttcc tggcccatta cctctacgcc cacatgaaag ggttgggcgg gttggaagtc
//
LOCUS       DAOQLS010000424         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_41915_length_1918_cov_4.744498, whole genome shotgun sequence.
ACCESSION   DAOQLS010000424 DAOQLS010000000
VERSION     DAOQLS010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41915_length_1918_cov_4.744498"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="pstA"
                     /locus_tag="PK362_07975"
     CDS             complement(<1..827)
                     /gene="pstA"
                     /locus_tag="PK362_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC74801.1"
                     /translation="MKRGWINFTSHAFTVLTGIACFGVILLVALILGNILWHGVGHIS
                     WAFLTTAPSAGMTEGGIFPAIFGTAFLVILMTIAVVPLGVSTAIYLQEYAPAHSRWVP
                     LVRMAIQNLAGVPAIVFGLFGLGFFIQFVGRGLDNFFFNKELYFGQPAILWAALTLAL
                     LTLPTVVVATEEALRVVPRAHREVAYSLGATRWQMVRHVLLPQATGGILTGTILAVSR
                     GAGEVAPILFTGAAYYLPYLPKKLNQQFMELGYHVYVMATQSPDVDATMPILYATVLV
                     "
     gene            complement(824..1759)
                     /gene="pstC"
                     /locus_tag="PK362_07980"
     CDS             complement(824..1759)
                     /gene="pstC"
                     /locus_tag="PK362_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNC74802.1"
                     /translation="MKARWSERLIEGAIRLSAGLAIVSLVLIFVFIGKETLPLFTSPE
                     ARAEAGMGRLFGTGSASTGDIATTYVWQPVSEEPKYSFWPLIAGTLKATAVALLFALP
                     LALGAALFTSEFAPPALREVVKPVIELLAGIPSVVLGFFALIVMATFFQNVFGLDYRL
                     NTWNAGFALGLAVIPIVYTVAEDALSAVPQSFRSGSIALGATPWQTARRVVLPAAFPG
                     LFAASVLGFGRAIGETMIVLMASGNAAILSWNPGLPFRSLSATVAAELGEVVHGSPHY
                     HVLFFIGVLLFVFTFLINLAGQVWVNRLQKRLVGK"
     gene            complement(1756..>1918)
                     /locus_tag="PK362_07985"
     CDS             complement(1756..>1918)
                     /locus_tag="PK362_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC74803.1"
                     /translation="SGKYAASRNLFFYLRAPATGDLKKFIDWVLSPEGQGVVSSVGYF
                     PLKKAAAKK"
BASE COUNT          397 a          571 c          677 g          273 t
ORIGIN      
        1 acgagcaccg tggcgtaaag gatcggcatg gtggcgtcca cgtccgggga ttgggtggcc
       61 atgacgtaca cgtggtagcc caattccatg aattgttggt tcagtttttt gggcaggtag
      121 ggcaggtaat aggccgctcc ggtaaagagg atcggcgcca cttcgcccgc gccgcggctc
      181 acggccaaaa tcgttccggt gaggatcccg cccgtggcct ggggcaaaag aacgtggcgg
      241 accatctgcc agcgggtggc gcccagggag taggccacct cccggtgggc ccgggggacg
      301 acccgcaggg cttcctcggt ggcgacgacg acggtgggaa gggtcaaaag cgccagcgtg
      361 agggcggccc agaggatcgc gggctggccg aaataaagtt ccttgttgaa aaagaaattg
      421 tcgaggcccc ggccgacgaa ctggatgaaa aaccccaggc cgaaaaggcc gaagacgatg
      481 gccggcacgc cggccaggtt ttggatggcc atgcgcacca ggggcaccca gcgggagtgg
      541 gcgggggcgt attcctgcag gtaaatggcg gtggagaccc ccaggggcac caccgcgatc
      601 gtcatgagga tgaccaggaa ggccgtgccg aagatcgccg gaaaaatgcc gccttcggtc
      661 atgccggccg agggggccgt ggtcaaaaag gcccaggaga tgtggccgac cccgtgccag
      721 aggatgttgc ccaggatcaa cgccaccaac aggatgacgc caaagcaggc gattcccgtg
      781 agaaccgtga aggcgtggct cgtgaagttg atccagcccc gcttcatttg cccaccagcc
      841 gtttttgcag ccggttcacc cacacctggc ccgcgaggtt aatgaggaag gtgaagacga
      901 agagcagaac gccgatgaaa aagagcacgt ggtagtgggg gctgccgtgc accacctcgc
      961 cgagctccgc cgccaccgtg gccgacaggc tgcggaacgg caggccgggg ttccaggaga
     1021 ggatggcggc gttgcccgag gccatcagga cgatcatggt ctccccgatg gcccggccga
     1081 agcccagcac cgaggccgcg aaaagacccg ggaacgccgc cggcaacacc acccggcggg
     1141 cggtctgcca gggcgtggcc cccagggcga tggacccgga ccgaaaactc tggggcacgg
     1201 cgctcaaggc gtcctcggcc acggtgtaga cgatggggat cacggcgagg cccagggcga
     1261 agcccgcgtt ccaggtgtta agccggtagt ccaggccgaa gacgttttga aagaaggtcg
     1321 ccatgacgat cagggcgaaa aaccccagca ccaccgaggg aatgcccgcc aaaagctcga
     1381 tcacgggctt gaccacctcc cgcagggccg gcggcgcgaa ctccgaggtg aaaagggccg
     1441 cccccagggc caggggcagg gcgaaaagga gcgccaccgc cgtggccttg agcgttccgg
     1501 cgatcagcgg ccagaaggaa tatttgggtt cttccgaaac gggctgccac acgtaggtcg
     1561 tggcgatgtc gccggtcgag gccgatccgg tgccgaagag ccgccccatc cccgcctcgg
     1621 cccgggcttc gggcgaggtg aaaaggggca gggtttcttt gccgatgaag acaaagatga
     1681 gcaccaggct cacaatggcg agcccggcgc tcaggcggat ggcgccttcg atcagccgtt
     1741 cggaccagcg ggctttcatt ttttcgcggc ggccttcttg agggggaaat aacccacgga
     1801 gctcacgacg ccttgaccct cgggcgacag gacccagtcg atgaatttct tgaggtcgcc
     1861 cgtggcgggg gcgcgcaggt aaaagaagag gtttcggctg gcggcgtatt tccccgag
//
LOCUS       DAOQLS010000425         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42017_length_1915_cov_7.532796, whole genome shotgun sequence.
ACCESSION   DAOQLS010000425 DAOQLS010000000
VERSION     DAOQLS010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42017_length_1915_cov_7.532796"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..239)
                     /locus_tag="PK362_07990"
     CDS             complement(60..239)
                     /locus_tag="PK362_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74804.1"
                     /translation="MEKSSAESMIDMVVDEKNREAWAVKNQAQVKAAVAAFDGFLGML
                     RETFKEDAKRREGLR"
     gene            complement(523..669)
                     /locus_tag="PK362_07995"
     CDS             complement(523..669)
                     /locus_tag="PK362_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74805.1"
                     /translation="MKRSRKEKELITKTGWPVVNLTTSAHKTYEYRLRRAGRGFVWEK
                     IGLR"
     gene            complement(679..1161)
                     /locus_tag="PK362_08000"
     CDS             complement(679..1161)
                     /locus_tag="PK362_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74806.1"
                     /translation="MRIGDFLNPAPVNWRRVLAISGMGVIVVLCLWVRLQSIALAREQ
                     LVYLHPRVENHVRVVRVEGPVRIVTRIVKTADREETVREEVRGPVVSTTEAVHIETPV
                     FPPPRPPRWVAGASLLDFRPMDRARWTAWAGVSVGGRLDLCLGVTGRLHTGAMVLYRF
                     "
     gene            complement(1335..1775)
                     /locus_tag="PK362_08005"
     CDS             complement(1335..1775)
                     /locus_tag="PK362_08005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC74807.1"
                     /translation="MPEFKKVASASPVPSKYKNKITKRVGDTGPMWSTFHKGTDFAVP
                     EGTPIYAAHGGTVVHSSNDGGKEGLYVSVDWLGADGMMRDIYFHLSRADAKVGKRVRT
                     GDTLGLSGKTGNTDGPHLHFQIQSGQGVERKFYVPAINEQAANA"
BASE COUNT          366 a          623 c          533 g          393 t
ORIGIN      
        1 ccccttgatg tattccttaa cccacggctg cggcgtgttt ttcgtttcgg ttttcgtttt
       61 catcgaagtc cctcccgccg cttcgcgtcc tctttgaacg tctcccgcaa catcccgagg
      121 aagccatcga aggccgcgac cgccgccttc acctgggcct gattcttcac cgcccatgcc
      181 tcccggttct tctcatccac caccatgtcg atcatcgact ctgcgcttga tttttccacg
      241 gttacctcca tgtgctgact caaaacagaa cgcccccgct tccgtgtgtg gtcggaaacg
      301 ggggcgtgga ctgccttctc tcgacgaagg aaagcaccca cggcggaagg caagttcccc
      361 tgccccttcg acccaccgtg ggaatgtggt caacaaaatg cccgccgctc gtcgagcgcc
      421 gggtcttgtg tttgatgggg tcgaaggttc ccatgcccat actctaaatc aaattcggta
      481 gcgagtcaag ggccttttaa aagatttaca actgcgctac aactacctca ggccgatttt
      541 ctcccacacg aatccgcgcc cggcccgacg gagacggtat tcgtaggtct tgtgcgccga
      601 cgtcgtgagg ttcacgacgg gccacccggt tttcgtgatg agttcctttt ctttgcgtga
      661 ccttttcatg gcgacctcct agaatcggta aagcaccatc gcgcccgtgt gcagtcttcc
      721 ggtgacgcca aggcagaggt cgaggcggcc gccgacggac accccggccc acgccgtcca
      781 ccgagccctg tccatagggc ggaagtccaa gaggctggcc ccggctaccc accgaggagg
      841 gcgcggcggc ggaaacacgg gcgtctcaat gtggaccgcc tcggtggtgg aaacgaccgg
      901 gccacggact tcttcccgca ccgtctcctc ccggtcggcc gtcttaacga tccgggtcac
      961 gatccgcacc gggccttcca cgcggaccac tcggacgtgg ttctcaacgc gggggtggag
     1021 gtagaccaac tgctcccggg ccagcgcgat ggattggagc ctgacccaca ggcaaaggac
     1081 gacgatgacg cccatgccgg agatggccag gacgcgccgc cagttaacgg gagccggatt
     1141 taggaagtcg cctattcgca aggagtttgt ccaagacggt gccgccgcca acaagagcgg
     1201 ccgccgtcgt cgtcgcccac acccagtcgc ccgccttaat caggccagcg atcaggagcc
     1261 cgtgcgcagt caagaaaagc cagaggccga taccgacttt ccttgaaccg ttgaataaga
     1321 gaaacgatac cactttaggc atttgccgcc tgctcattga tcgcggggac gtaaaatttg
     1381 cgctcgacgc cctgcccgct ctgtatctgg aaatgcaggt gtgggccgtc ggtgttcccg
     1441 gtttttccag acaggccaag cgtgtcaccc gtccgaaccc tcttcccgac cttggcgtca
     1501 gcgcgggaga gatggaagta aatgtccctc atcatcccat cggcccccag ccagtcgacg
     1561 gaaacgtaaa gcccttcctt cccaccgtcg tttgaactgt ggacgacggt cccgccgtgg
     1621 gcggcgtaga taggcgtccc ctccggcacg gcgaagtcgg tccccttgtg gaacgtcgac
     1681 cacatcggcc ccgtgtcacc cacacgcttc gttatcttgt tcttgtattt agaagggaca
     1741 ggggaagcgg acgccacctt cttgaattca ggcatttttc ctcccaaagt attcctcgta
     1801 gaatcgatcg gtttgggaca acttctgatg gaggatgatg cggtggacga caccccccct
     1861 gacgtaatgg aagtcgacgc tcggttcaca ccggcaattc ccgggaccgc cgaag
//
LOCUS       DAOQLS010000426         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42178_length_1911_cov_3.836207, whole genome shotgun sequence.
ACCESSION   DAOQLS010000426 DAOQLS010000000
VERSION     DAOQLS010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42178_length_1911_cov_3.836207"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /gene="panC"
                     /locus_tag="PK362_08010"
     CDS             complement(<1..805)
                     /gene="panC"
                     /locus_tag="PK362_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNC74808.1"
                     /translation="MKIVRRPSDCQKQALAWRAAGRTIGLVPTMGALHEGHASLLRRA
                     RRENDRVIATVFVNPAQFGPAEDFKKYPRPFARDAALCRAAGVDLLLAPRPESMYAAD
                     HDAWVTVPRTAAPLCGAFRPGHFRGVATVVAQLFNLAQPTRAYFGAKDYQQTRVVARL
                     AADLHFPVKVVVCPTVREASGLARSSRNRYLSDAERTAAPALWRALRSAAQVLKSRRN
                     AAAARRAARAELARVPGLRPQYIDVVDPRSLAPWRSGPAVIAAAVFLGRT"
     gene            complement(802..1719)
                     /locus_tag="PK362_08015"
     CDS             complement(802..1719)
                     /locus_tag="PK362_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC74809.1"
                     /translation="MILVLAGLSLLFFVLWAYFSSALIIDGNRDPITVLPRTFGLEFT
                     EEAFPSADGLTLKGWFVPAQGPRARGKPGPVLPTDKTLIVCHGWGANRSDILERTYFL
                     NTEGGYNLFYFDFRNHGDSGSGKSSLTKLEIGDLEAALSHLRAAHPAEARRVALYGMS
                     LGAAVCLWVAAQHPDIRGVAAESPFGSYRDVVIRFGRMYYNMPRVPFALITLWAVRWR
                     LGFDPQDYSPLAVVGKIAPRPLFLLQGDKDARMPVMEGERLFAAAGEPKTLWTVPGAN
                     HGEVAEWGGRAYRQKLLDFYRGVFEGPTR"
     gene            complement(1761..1901)
                     /locus_tag="PK362_08020"
     CDS             complement(1761..1901)
                     /locus_tag="PK362_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74810.1"
                     /translation="MGLTPPPRPRFARAYAELGAESLRAVRAYAGDVAAGRFPGPRES
                     AD"
BASE COUNT          334 a          629 c          683 g          265 t
ORIGIN      
        1 gggtccgccc caggaaaacg gccgcggcga tcaccgccgg tccggaacgc cagggggcca
       61 aggaccgcgg gtccaccaca tcgatgtact ggggccgcag gcccggaacc cgcgcgagct
      121 cggcccgagc cgcgcgacgg gcggccgccg cgttccgtcg ggattttaac acttgggcgg
      181 cggagcgtaa agcgcgccac aacgcggggg ccgccgtccg ctccgcgtcg gacaaatagc
      241 ggttgcggct cgagcgcgcc agcccggagg cttcgcgcac ggtggggcac acgacgacct
      301 tcacggggaa atgcaggtcg gccgccagcc gggccacgac ccgcgtttgc tggtaatcct
      361 tggccccgaa gtaggcgcgg gtcggttgcg cgagattgaa cagctgggcc accacggtcg
      421 ccaccccccg gaaatgcccc gggcggaagg ccccgcacag gggcgcggcg gttcggggaa
      481 cggtcaccca ggcatcgtgg tccgccgcgt acatcgattc cgggcgcggg gccagcaaca
      541 ggtccacgcc cgccgcccgg cagagagccg cgtcccgggc gaagggccgg gggtatttct
      601 tgaaatcctc cgcgggcccg aattgggcgg gattcacaaa gacggtggcg atgactcggt
      661 cgttttcccg gcgcgcccgc cggagaagcg acgcgtggcc ctcgtgaagg gcccccatgg
      721 tcgggaccaa accgatggtg cgaccggcgg cgcgccaggc cagggcctgt ttttggcaat
      781 ccgacggccg tcggacgatt ttcaccgggt cggcccctcg aaaaccccgc ggtagaaatc
      841 cagcaatttt tgccgatagg cccgtccgcc ccattcggcc acctcgccgt gattggcgcc
      901 gggcaccgtc cagagggttt tgggttcccc ggccgcggcg aacaggcgtt ccccttccat
      961 gaccggcatg cgggcgtcct tgtccccctg aagcagaaac agcgggcggg gcgcgatttt
     1021 tccgacgacc gccaagggcg agtaatcctg ggggtcgaac cccagccgcc atcgaacggc
     1081 ccacagcgtg atcagggcga aagggacccg gggcatgttg tagtacattc ggccgaaccg
     1141 aatcacgacg tcgcggtagg agccgaaggg gctttccgcg gccacgcccc gaatgtccgg
     1201 gtgctgcgcc gccacccaca gacacacggc cgcgcccagg gacatgccgt agagcgcgac
     1261 ccgacgggcc tcggcgggat gggcggcccg caggtgggac agcgccgcct ccaggtcgcc
     1321 gatttcgagc ttggtcagcg aggattttcc ggacccgctg tccccgtggt ttcggaaatc
     1381 gaaataaaaa aggttgtagc ccccctcggt gtttaagaaa tacgtccgct ccaggatgtc
     1441 cgaccggttg gcgccccatc catgacagac gatgagtgtt ttgtccgtgg gaaggacggg
     1501 gccgggtttc ccgcgagccc gggggccctg ggccgggacg aaccatcctt tcagcgtcag
     1561 gccgtcggcg gaggggaacg cctcctcggt gaattcaagt ccgaaggtcc ggggaaggac
     1621 ggtgatgggg tcccgatttc cgtcgatgat cagggccgag gaaaaatagg cccagaggac
     1681 gaaaaaaagg agggacagcc cggcgagaac gaggatcatg cggccgaggg tttggagaaa
     1741 ttcacgggca cggcctccga tcaatcggcg gactcgcgcg gcccggggaa tcggccggcg
     1801 gccacgtccc cggcgtaggc gcggaccgcg cggagactct cggcgcccag ctccgcgtag
     1861 gcccgtgcga aacgcgggcg gggcggcggg gtgagtccca acagatcgtc c
//
LOCUS       DAOQLS010000427         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42188_length_1911_cov_3.275323, whole genome shotgun sequence.
ACCESSION   DAOQLS010000427 DAOQLS010000000
VERSION     DAOQLS010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42188_length_1911_cov_3.275323"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK362_08025"
     CDS             <1..433
                     /locus_tag="PK362_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNC74811.1"
                     /translation="FIRPEWVESVAGRNTIGGNLAGVVFGVFMYFPTLVEVPIAKMFL
                     SLGMHRGPLIAYLMADPELSLQSILIVSTVIGRVKAWTYVAWVALFSMASGLLYGAWV
                     DGASACRLGAGLIFFIGTLLGSLAWASRRATRRVSENERGG"
     gene            438..671
                     /locus_tag="PK362_08030"
     CDS             438..671
                     /locus_tag="PK362_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC74812.1"
                     /translation="MKKIQILGTGCPKCKKLTESAEAAAKDLGIEYTLEKVTDIEKIM
                     SYGVMMTPALAVDGQVKISGRVPSVDEVKKLLS"
     gene            683..1117
                     /locus_tag="PK362_08035"
     CDS             683..1117
                     /locus_tag="PK362_08035"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNC74813.1"
                     /translation="MRRLIRLVLAGAFCAGMGASAGSVKINVSKIENAQLIVYYFHGT
                     ARCVTCRKFETWTQEVLTSSFVDEIKKKRLEYRVVNIDEPGNEHFVHDYGLYTKSVVL
                     SEIKDGRRTRSKNLEKIWDLARDEEAYKAYVRDEVSSFIAKG"
     gene            1117..1812
                     /locus_tag="PK362_08040"
     CDS             1117..1812
                     /locus_tag="PK362_08040"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNC74814.1"
                     /translation="MESFPLASWTALWLGVLTSLSPCPLATNITAVAYIGRLVGRPRA
                     VLAAGLLYTVGRSAAYLLLCAGVALGLLSIPGLSQFLQRRMNQALGPLLILTGILLFT
                     VDRFTAGGTGRWVASAQRWADKGGVWGAGLLGFVFAASFCPLSAALFFGSLVPLTVSH
                     RSPLLLPSLYGLGTAIPAVILAVTLSVGGTLVARVYNRMSGLEKWGRRLTGGLFILVG
                     VYLTLIHVFGVQF"
     gene            1812..>1911
                     /locus_tag="PK362_08045"
     CDS             1812..>1911
                     /locus_tag="PK362_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNC74815.1"
                     /translation="MAIKRKILFLCTGNSCRSQMAEGWARHLLGETI"
BASE COUNT          347 a          525 c          600 g          439 t
ORIGIN      
        1 gttcatccgg ccggaatggg ttgagtcggt cgccgggcgc aacacgatcg gcggcaacct
       61 cgccggtgtc gtcttcggcg tcttcatgta tttcccgacg ctggttgaag tgcccatcgc
      121 caagatgttt ctttccctcg gaatgcaccg ggggccgctc atcgcctatt tgatggccga
      181 cccggagctg agcttgcaaa gcattttgat cgtgtccacc gtgatcggcc gcgtgaaagc
      241 gtggacgtac gtcgcctggg tggcgctgtt cagcatggct tccgggctcc tctacggcgc
      301 atgggtggac ggggcttccg cgtgtcggtt gggcgcgggc ctgatatttt tcatcggaac
      361 cctgctcgga agtttggctt gggccagtcg ccgagccacc cggcgcgttt ccgaaaatga
      421 gcgcggagga tgaacccatg aaaaagatcc aaatcctggg gacgggttgt cccaaatgca
      481 aaaagctgac ggaaagcgcc gaggcggcgg ccaaggacct gggcattgaa tacacgctgg
      541 aaaaagtaac ggacattgag aaaatcatgt cctacggtgt gatgatgacc cccgcgttgg
      601 ccgtcgacgg tcaagtgaag atatccggcc gcgttcccag cgtggacgag gtgaagaagc
      661 tcttatcttg aggaggacgc tgatgcgacg attgatccga ttggttttgg ccggggcttt
      721 ttgcgccggg atgggcgcgt ccgcggggtc cgtcaagata aacgtctcaa aaattgaaaa
      781 cgcacaactc atcgtctact actttcacgg cacggcccgg tgcgtcacct gtcgaaagtt
      841 tgaaacctgg acgcaggagg tgttgacctc ttcttttgtc gacgaaatca aaaagaaacg
      901 cctggaatac cgcgtggtga acattgacga acccggcaat gagcatttcg tccatgatta
      961 cgggctttac accaaatccg tggtcctttc ggaaatcaag gacggccgcc ggacgcgctc
     1021 caagaacctt gaaaaaatct gggatttggc ccgcgacgaa gaagcctaca aggcctatgt
     1081 ccgcgacgaa gtgtcgtcgt ttatcgcgaa gggctgatgg aatcttttcc cctcgcgtcc
     1141 tggacggcgc tttggctggg ggtcctcacc tctttaagcc cttgcccctt ggccaccaac
     1201 atcacggcgg tggcctacat cggacgtctt gtgggacgac cgcgcgccgt tttggcggcg
     1261 ggactccttt acaccgtcgg acggtcggcg gcctacctct tgctgtgcgc gggagtggcc
     1321 ctcgggcttt tatcgattcc gggattgtcc cagtttcttc aacggcggat gaatcaagcc
     1381 ttggggccgt tgctgattct cacggggata ctcttgttca ccgtcgatcg tttcaccgcc
     1441 ggggggacgg gacgatgggt ggcctccgcg caacgatggg ccgacaaggg cggggtctgg
     1501 ggcgcggggc tcttggggtt cgttttcgcg gcgtcctttt gtcccttatc ggcggcgctc
     1561 ttttttggga gtctcgttcc gctcaccgtg agtcaccggt cgccgctttt attgccgtcg
     1621 ctctacggcc ttgggacggc catccccgcc gtgattctgg ccgtcacgct gtccgtgggc
     1681 ggaacgctgg tggcccgcgt ctacaaccgc atgagcggcc tggaaaaatg ggggcgtcgg
     1741 ctcacgggcg gtttgttcat tctcgtcggg gtttatctga cgttgatcca tgtcttcggg
     1801 gtgcaattct aatggcgatc aaacggaaaa tcttatttct ttgcacgggc aattcttgcc
     1861 ggagccaaat ggccgaagga tgggcgcggc atttgctggg agaaacgatc g
//
LOCUS       DAOQLS010000428         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42206_length_1911_cov_1.679418, whole genome shotgun sequence.
ACCESSION   DAOQLS010000428 DAOQLS010000000
VERSION     DAOQLS010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42206_length_1911_cov_1.679418"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..802)
                     /locus_tag="PK362_08050"
     CDS             complement(101..802)
                     /locus_tag="PK362_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC74816.1"
                     /translation="MPFKPDPWLSRVLGRPVHRGVGRRFRPPSRWAKGFYHARIDADR
                     GTDLRAALRAGFRLAEVAISLERSPARERTAGGPVVRPLSSADAPGLRSWGGGAFVYS
                     RFHLDPAFARAEATAVKRAWLEEYLAGRRADRVWVALDRGRPVGFLGRCRRVEDKRRV
                     GVLDLMAVRPSHRGRGVGRALVRRFVRDSAADCDLLRVGTQAANAPSLRLYGACGFSV
                     RGAQFVVHAHRGGRG"
     gene            complement(792..1823)
                     /locus_tag="PK362_08055"
     CDS             complement(792..1823)
                     /locus_tag="PK362_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008313755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC74817.1"
                     /translation="MTNEIKGRRILLLGGAGFIGSNLALSLKAQGAEVHVVDGLQVNN
                     LLYYSSRDHEGPNREMYVSFINERLRLLQDAGVPLWVEDLRDYHALARTFDKIKPQTV
                     VHLAAVAHAGKSNKDPFSTFDHSLRTLENALDCSRGRIEHFVYFSSSMVYGNFRTPEV
                     TEDHPLDSIGIYGALKVAGEKMVIAYQQVFDLPYTVIRPSALYGERCVSRRVGQVFIE
                     SALAGKPLRVDGDGSERLDFTYIGDLVKGVELAMVAPAARNEIFNITHGQSRSVQELI
                     EVVRSEFPNIKVERVERDKLMPFRGTLNVEKARRLLGYAPAFPIEKGFRDYIAWYKKR
                     PKAPGTNAV"
BASE COUNT          338 a          658 c          614 g          301 t
ORIGIN      
        1 gcctcgagca atcggaacgc ccggtccgcg tcgccctcgt ggttgttgcc gatttcggcg
       61 acgaccatca cgcgttccgc cgtgtcgaac ccgccgatct tcacccgcga cctccccggt
      121 gggcgtggac gacgaattgg gccccgcgga ccgaaaaccc gcacgccccg taaagccgca
      181 gggacggcgc gttggcggcc tgggttccca cgcgcagaag atcgcaatcc gccgcgctgt
      241 cgcggacgaa acgccgcacc agcgcccgac cgacgccgcg cccgcggtgg gacggccgca
      301 ccgccatcaa atcgagaacg cccacccggc gtttgtcctc cacgcggcgg caccgtccca
      361 ggaaacccac gggtcgcccc cggtcgaggg ccacccaaac gcggtcggcc cgccgaccgg
      421 cgaggtattc ctccagccaa gcgcgcttga cggcggtcgc ctcggcccgg gcgaaggccg
      481 ggtccaaatg aaaccgggaa tagacgaagg cgcctccgcc ccaggaccgg aggccggggg
      541 cgtccgcgct cgacaggggt cgcacgaccg gcccgccggc cgttcgctcc cgggccgggg
      601 agcgctccag cgaaatcgcg acctccgcca ggcggaagcc ggcccggagc gcggcccgaa
      661 ggtcggtccc ccggtccgcg tcgatacggg cgtgataaaa ccccttcgcc cagcgcgacg
      721 gcgggcggaa acggcggccc acgccgcggt ggacggggcg cccgagcacg cgcgacagcc
      781 aggggtcggg cttaaacggc attcgtcccc ggggccttcg gacgcttttt gtaccacgcg
      841 atataatccc gaaaaccctt ctcgatgggg aaggcgggcg cgtagcccaa caggcgacgg
      901 gctttttcca cgttcagcgt gccccggaac ggcatgagtt tgtcccgctc gacacgctcg
      961 actttgatgt tcggaaactc ggaacggacc acctcgatca attcctggac ggaccgggat
     1021 tggccgtggg tgatgttgaa gatctcgttg cgcgccgcgg gggcgaccat ggcgagttcc
     1081 acgcctttca ccaaatcccc gatgtaggtg aagtccaacc gctcgctccc gtcgccgtcc
     1141 acgcgaaggg gctttccggc cagggcgctt tcgatgaaca cctgcccgac gcgccggctg
     1201 acgcagcgct cgccgtaaag cgccgacgga cgaatgacgg tgtagggcag atcgaacacc
     1261 tgttggtacg cgatcaccat tttttccccg gccactttca acgccccgta aatgccgatc
     1321 gaatcgaggg gatggtcctc cgtcacctcg ggggtgcgaa aattgccgta gaccatgctg
     1381 gaggaaaaat acacgaagtg ctcgatgcgt ccgcggctgc agtccagggc gttttcgagc
     1441 gtccgcaggc tgtgatcgaa ggtgctgaag gggtccttgt tggatttgcc ggcgtgggcc
     1501 accgccgcca agtggacgac cgtctgcggc ttgatcttgt cgaaggtgcg ggcgagggcg
     1561 tggtaatccc ggaggtcctc gacccacaac ggcacgccgg cgtcctgcag caggcgcaac
     1621 cgttcgttga tgaaactcac gtacatctcc cggttgggcc cttcgtggtc ccgggaggag
     1681 taataaagca ggttgttcac ctgcaacccg tccacgacgt ggacctccgc cccctgggcc
     1741 ttcagactca acgccaggtt ggaaccgata aagccggcgc cgcccaaaag aaggatccgg
     1801 cggcctttga tttcgttcgt catgatttca cctcgttcaa ggtttcgata aattcgcgcg
     1861 ccacccggtc cacgtccgcc tccgtcaaat gggggccgac cggcaacgcc a
//
LOCUS       DAOQLS010000429         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42224_length_1910_cov_3.984906, whole genome shotgun sequence.
ACCESSION   DAOQLS010000429 DAOQLS010000000
VERSION     DAOQLS010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42224_length_1910_cov_3.984906"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK362_08060"
     CDS             <1..314
                     /locus_tag="PK362_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HNC74818.1"
                     /translation="MASRPPPAAIAAPPAAAPSAPAPAGQAITSPLAGVFYRSPAPNA
                     PAFVKEGDTVAYGDVLCIVEAMKVMNEIRADKPGKIKKIAAENGKPVSAGQDLFLLEP
                     A"
     gene            320..520
                     /locus_tag="PK362_08065"
     CDS             320..520
                     /locus_tag="PK362_08065"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC74819.1"
                     /translation="MHHEIGQAAGLVLDLLTRHGECSALKIRSDLRMTQTALYLALGW
                     LSREGKVDIVARDRTYWVVAKP"
     gene            517..1302
                     /locus_tag="PK362_08070"
     CDS             517..1302
                     /locus_tag="PK362_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNC74820.1"
                     /translation="MTVLRAALLGLVQGLTEFLPVSSDGHLALFQAAFGEGSLALDVA
                     LHAGTLLSMLLFFRSDLVRLLAGLTSRTDDEPRALERRRLLLIVIATVPTGLIGLGLK
                     HTVERLSMSLVAAGLGFLITAAFLLAGERRGRREGGLSLRDIPLWHPVVIGLAQGAAV
                     WPGWSRSASTIGLALVLGWRWDDAGRFSFLAAMPAIFGAILLTARDIAALPPAPAAVG
                     FLVSFAVGTLSLKILMRFLAARRLWPFALYCLGLGLFALLKSV"
     gene            1314..>1910
                     /locus_tag="PK362_08075"
     CDS             1314..>1910
                     /locus_tag="PK362_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNC74821.1"
                     /translation="MSFFLNARFKTNARGRRRTESKNRGSVPGVVLMTASVLAVYCVL
                     FPSGLWGKPIKGALAAAFGVGRFFVPLWTGYVGLRFSLRRPWPQPTLRIVLGAILQVA
                     ALSFVSLFSQMAFQQNAAGVVGLAGNEFLARLFGVPGAWLVSLTAVALSTAGLLRVSP
                     VTVVEWLWTQLKNDVDEWRTARQQSAVSKKEPVVKPRPL"
BASE COUNT          263 a          691 c          558 g          398 t
ORIGIN      
        1 ccatggcttc ccgccccccg cccgcggcga tcgccgcccc tccggcggcg gccccgtcgg
       61 cccccgcgcc cgcggggcaa gccatcactt cgcctttggc gggggtgttc taccgctccc
      121 cggcccccaa cgccccggcc tttgtcaaag agggggacac cgtggcctac ggcgacgtgt
      181 tgtgcatcgt tgaagccatg aaggtgatga acgagatccg cgccgacaaa cccggcaaaa
      241 tcaagaaaat cgccgcggaa aacggcaaac cggtttccgc ggggcaagac ctcttcctcc
      301 tcgaaccggc ctgaccggga tgcaccacga aatcggccag gccgccggcc tcgtgctcga
      361 ccttctgacc cgccacggcg agtgttcggc ccttaagatc cgctccgacc tccgtatgac
      421 ccaaacggcc ctttacctcg ccctcggctg gctctcccgg gaagggaagg tcgatatcgt
      481 cgcccgggac cgaacctact gggtggtcgc caaaccgtga ccgtccttcg cgccgctctg
      541 ctcggtctgg tccaaggcct gaccgaattc ttgcccgttt cctccgacgg ccatctggcc
      601 ctatttcaag cggctttcgg cgaaggttcc ctcgccctgg acgtcgccct gcacgcgggg
      661 acccttcttt ccatgctgct tttcttccgt tccgacctcg ttcgtctcct ggcgggcttg
      721 acctcccgga ccgacgacga accccgcgcc ctggaacgac ggcggcttct attgatcgtg
      781 atcgccacgg ttcccaccgg cctcatcgga ctgggcctga aacacaccgt tgaacgcctc
      841 tccatgtccc ttgtggcggc gggtctcggt tttctcatca ccgcggcctt tctcctggcg
      901 ggcgaacgcc gggggcggcg ggagggcggg ttgtcccttc gcgacattcc gctttggcac
      961 cccgtggtga tcggcctggc ccaaggggcc gccgtctggc ccggatggtc ccgaagcgcc
     1021 tccaccatcg gcctggcctt ggttttggga tggcgttggg acgacgccgg ccgattcagt
     1081 tttttggccg ccatgccggc cattttcggg gccatccttt tgaccgcccg ggacatcgcg
     1141 gccctaccgc ccgcgcccgc cgccgtgggg ttcctggtgt ccttcgcggt cgggaccctg
     1201 tcgctcaaaa tcctgatgcg ttttctcgcc gcccgccgcc tctggccctt cgccctttat
     1261 tgcctcggct tgggcctctt cgcccttctc aaaagtgtgt aaaataagag tccatgtcct
     1321 ttttcctgaa cgcccgattt aaaaccaacg cccgcggtcg ccgccggacg gaatccaaaa
     1381 accgcgggtc cgtgccgggg gtcgttttga tgacggcctc ggtgctggcc gtttactgcg
     1441 tgctctttcc ctccggtttg tggggaaaac cgatcaaagg cgcgttggcc gcggccttcg
     1501 gcgtgggccg gttcttcgtt cccctctgga ccggttacgt ggggcttcgc ttttcgctcc
     1561 gtcgcccctg gccccaaccc accctgcgga tcgtcctggg cgccatcctt caagtcgccg
     1621 ccctttcgtt cgtcagcctc ttttcccaga tggcctttca acaaaacgcc gccggcgtcg
     1681 tcggattggc cggaaacgaa tttctggccc gcctgttcgg cgttccgggc gcctggctgg
     1741 tttccctgac ggccgtggcc ctgtccaccg ccgggctcct gcgcgtgtcc ccggtgaccg
     1801 tcgtcgaatg gctttggacg cagcttaaaa acgacgtgga cgaatggcgg accgcgcgcc
     1861 aacaatcagc ggtctcgaaa aaagaaccgg tcgtgaaacc ccggcccttg
//
LOCUS       DAOQLS010000430         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42317_length_1908_cov_3.297895, whole genome shotgun sequence.
ACCESSION   DAOQLS010000430 DAOQLS010000000
VERSION     DAOQLS010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42317_length_1908_cov_3.297895"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PK362_08080"
     CDS             complement(<1..469)
                     /locus_tag="PK362_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNC74822.1"
                     /translation="MKKNRPSDAARVRLYDTTLRDGTQGEGVSLTVDDKLKIAQVLDR
                     LGVDYIEGGWPGSNPKDEEFFKRARSLRLKNARLAAFGSTRRRDAAAHQDPNLAAIVR
                     VKTPVACIFGKSWDLHVVHALRATLDENLKMIGESVKFLKSKGKEVVYDAEHFF"
     gene            complement(466..1704)
                     /locus_tag="PK362_08085"
     CDS             complement(466..1704)
                     /locus_tag="PK362_08085"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNC74823.1"
                     /translation="MSSIVVMKFGGSSVADADRIKNVAGRVAAKRKQGHRVVVVVSAP
                     GDMTDDLIAMAEKITDSPSGREMDMLLSTGEQVSIALLSMAINERGVAAVSLTGPQAG
                     IFADQDHTRARITAIRPKKIFEQLKKGKVVIVAGFQGLNPNEDIATLGRGGSDLTAVA
                     LAAALKADVCEIYTDVKGVYTTDPRVEPEAQKIDRISYDEMLELAGAGAQVMQARSIE
                     VGKKYGVDIHVRSTFSEDEGTLITAEVPRMEQIVVSGVAFDPKQAKITLSGVADRPGV
                     AAKIFGPLADEGVNVDMIIQSAPTEGRNDISFTIGRVDVKKAMGVLDRVSTDLKAEEV
                     VCDDKVAKVSIVGVGMKGHPGVAAKLFKSLAEAGINIDMISTSEIKIACVVREGDGPR
                     AVRLVHKAFGLEKPRVKAKA"
     gene            complement(1701..>1908)
                     /locus_tag="PK362_08090"
     CDS             complement(1701..>1908)
                     /locus_tag="PK362_08090"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNC74824.1"
                     /translation="ARRAGAHGAALSGAGPSVLALCAPAAAPRVARAMVAAFGRKGSR
                     ARSEILKIDTAGARVFGVSRKRTK"
BASE COUNT          282 a          628 c          624 g          374 t
ORIGIN      
        1 cgaagaaatg ctcggcatcg tacaccactt ccttgccctt ggatttgaga aatttgaccg
       61 actcgccgat catcttcagg ttttcgtcca aggtcgcccg cagggcgtgg accacgtgga
      121 ggtcccagga tttgccgaaa atgcaggcca ccggggtttt cacccgcacg atggcggcca
      181 ggttggggtc ttggtgggcg gcggcgtccc ggcggcgggt gctgccgaag gccgccagac
      241 gcgcgttttt gagccgcagc gaccgggcgc gcttgaagaa ttcctcgtcc ttggggttcg
      301 agccgggcca cccgccctcg atgtagtcga cccccaggcg gtccagcacc tgggcgatct
      361 tcaacttatc atcgacggtg agcgagaccc cttccccctg ggtgccgtcc cgcagggtgg
      421 tgtcgtagag gcggacccgg gccgcgtcgg acggacggtt tttcttcacg ctttggcttt
      481 gacccggggt ttttccaagc cgaaagcctt gtgcacgagc cggacggccc gggggccgtc
      541 gccttcgcgc accacgcagg cgatcttgat ctcggaggtc gagatcatgt cgatgttgat
      601 cccggcctcg gccagggatt taaagagttt cgccgccacg ccgggatggc ccttcatgcc
      661 gacgcccacg atggacacct tggccacctt gtcgtcgcag accacttcct cggccttcag
      721 gtccgtggac acgcggtcca ggacgcccat ggctttcttc acgtccaccc gcccgatggt
      781 gaaggagatg tcgttgcggc cctccgtcgg ggcggactgg atgatcatgt cgacgttcac
      841 gccctcgtcg gccagggggc cgaagatttt cgccgcgacg ccgggccggt cggccacgcc
      901 gctcagagtg attttggctt gcttggggtc gaaggcgacc ccgctgacca cgatctgttc
      961 catacgcggc acctccgccg tgatgagtgt tccttcgtct tccgaaaacg tcgagcggac
     1021 gtggatgtcc acgccgtatt tcttgccgac ttcgatggac cgcgcctgca tgacctgggc
     1081 gcccgcgccc gcgagctcca gcatttcgtc gtaggaaatg cggtcgatct tctgggcttc
     1141 gggttccacc cgcgggtccg tcgtgtagac gcctttgacg tcggtgtaga tttcgcagac
     1201 gtcggctttg agcgccgccg ccagggccac ggccgtcaag tccgaccccc cgcggcccag
     1261 ggtcgcgatg tcctcgttgg ggttcaaccc ctggaacccg gccacgatga cgaccttgcc
     1321 ttttttaagc tgctcgaaaa tctttttggg gcggatggcc gtgatgcgcg cccgggtgtg
     1381 gtcctggtcg gcgaagatgc cggcctgggg ccccgtcaag gacaccgccg cgacgccccg
     1441 ctcgttgatg gccatggaca ggagcgcgat ggagacctgc tcgcccgtcg acaacaacat
     1501 gtccatctcg cggccgctcg ggctgtcggt gattttttca gccatggcga ttaaatcgtc
     1561 cgtcatgtcc ccgggggcgg agacgaccac gaccacgcgg tggccctgtt tgcgcttggc
     1621 ggccacgcgg ccggccacgt tcttgatgcg gtcggcgtcc gccacggaac tgccgccgaa
     1681 tttcataacg acgatggaac tcatttcgtt ctctttcgtg agacgccgaa aacgcgggcg
     1741 ccggctgtgt cgatcttcaa aatttcgctg cgggcccggg accctttccg tccgaaggcc
     1801 gcgaccatcg cccgggccac ccggggcgcc gcggcggggg cgcagagggc caaaacgctg
     1861 gggcccgcgc cggacagggc cgcgccgtgg gcgccggccc gacgggcg
//
LOCUS       DAOQLS010000431         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42766_length_1898_cov_4.514379, whole genome shotgun sequence.
ACCESSION   DAOQLS010000431 DAOQLS010000000
VERSION     DAOQLS010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42766_length_1898_cov_4.514379"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK362_08095"
     CDS             complement(<1..227)
                     /locus_tag="PK362_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNC74825.1"
                     /translation="MARAAGGRSTARGMDCVVVTGLSGAGKTSALRALEDMGYFCVDN
                     LPLPLLPSLLRLYRTWGHRLTRIAVGVDVRTG"
     gene            complement(239..1219)
                     /gene="hprK"
                     /locus_tag="PK362_08100"
     CDS             complement(239..1219)
                     /gene="hprK"
                     /locus_tag="PK362_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNC74826.1"
                     /translation="MTTGDSAAAAKSAPLHIEEFWKNQADALRLSLLAGQSGFRRVVR
                     VADINRPGLTLAGFFEYYRGERIQVFGMGESAFLSTLSSAQRMEIFDRILSVPELPCL
                     IITHGREPVPEMLEQCDRYGVPLFQSQWETARFIGETTAYLEERLAPCTSVHGTLVNV
                     YGMGVLILGHSGIGKSECALELLKRSHMLVADDRVEIRHKPGGILVGSGHQLVQHHME
                     VRGLGIIDVWKIFGVGAVLQRSLIELVVYLEMWDQKKEYDRMGLEEKNTTLLGVHVPE
                     IRVPVHPGRNLGSLIEVAALNQRLKQQGIHSARELNERLIQRMSGHSGRA"
     gene            complement(1224..1682)
                     /locus_tag="PK362_08105"
     CDS             complement(1224..1682)
                     /locus_tag="PK362_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNC74827.1"
                     /translation="MKLIDILSPQAISVDLAARTKKDALEEMCRLLAQAGKLPDPAAM
                     LKTLVDREALGSTGIGQGVAIPHGKTAAVSEQAAALGISKRGIDFDALDGEPVHIVFL
                     LTAPVDAAGNHLKALAKVSRLLKDKFFRQALRDAKAAEEILKIIREEDEY"
     gene            complement(1715..>1898)
                     /locus_tag="PK362_08110"
     CDS             complement(1715..>1898)
                     /locus_tag="PK362_08110"
                     /inference="COORDINATES: protein motif:HMM:NF027645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HNC74828.1"
                     /translation="REPVLTRISRVTPKYLSVAAAAQELGDSEFDFLLFFDESETLKV
                     IYRRKDGTYGLLEPNL"
BASE COUNT          348 a          597 c          607 g          346 t
ORIGIN      
        1 cccgtccgga cgtcgacgcc cacggcgatg cgggtcaggc gatggcccca ggttcggtag
       61 aggcgaagca gggagggcag gagcggcagg gggaggttgt cgacgcagaa ataacccatg
      121 tcctccaggg ctcggagggc ggaggttttt cccgccccgg agagtccggt gacgaccacg
      181 caatccattc cccgggcggt ggaccgaccg ccggcggcac gggccacgga ggttcccgtc
      241 aggcccggcc ggagtggccg gacatgcgct ggatcaacct ctcgttcaac tcccgggcgg
      301 aatggatgcc ctgctgcttg aggcgctgat tgagcgccgc gacctcaatc aaggatccca
      361 ggttccgccc cggatgcacg ggcacgcgga tttccggcac gtggacgccc aggagagtgg
      421 tgtttttctc ctccaggccc atgcgatcgt attccttttt ctggtcccac atttccaaat
      481 agacgaccaa ctcgatcagg gagcgttgaa gcaccgcccc caccccgaaa atcttccata
      541 cgtcgatgat gccgagcccg cgcacctcca tgtggtgctg gaccagctgg tggcccgacc
      601 ccaccaaaat gccgccgggc ttgtggcgga tttccacgcg gtcgtcggcc accagcatat
      661 gagagcgctt caggagttcc agggcgcact cggatttgcc gatgcccgag tgacccaata
      721 tcaatacgcc catgccgtag acgttcacca gggtcccgtg cacggaggtg cagggggcca
      781 ggcgctcttc caaataggcc gtcgtctcgc cgataaagcg ggcggtttcc cattgggatt
      841 gaaatagggg aacgccgtaa cggtcgcact gctccagcat ttcgggaacg ggttcgcggc
      901 cgtgggtgat gatcagacag ggcagttcgg ggacggacaa aatgcggtcg aaaatttcca
      961 ttcgctgggc gctggacaag gtggacagga aggccgattc gcccatgccg aacacctgga
     1021 tccgctcccc gcggtagtat tcaaaaaacc ccgccagggt caggcccggc cggttgatgt
     1081 ccgccacgcg tacgacgcgc cgaaaccccg actgcccggc caacaacgac aggcggaggg
     1141 cgtcggcctg gtttttccag aattcttcga tgtgcagcgg cgcggatttc gccgcggccg
     1201 cggagtcgcc ggtcgtcacc gtttcagtac tcgtcttctt cccgaatgat tttaaggatt
     1261 tcctcggcgg ccttcgcgtc gcggagggcc tgccggaaaa atttgtcttt caacaggcgc
     1321 gacaccttgg ccaaggcttt caaatggttg ccggcggcgt ccacgggcgc ggtcagcagg
     1381 aacacgatgt gcacgggttc gccgtccaag gcgtcaaaat cgatcccgcg cttcgaaatg
     1441 cccagggccg ccgcctgttc ggacaccgcc gccgttttgc cgtggggaat cgccacgccc
     1501 tggccgatgc ccgtggaccc gagagcctcc cggtccacca gcgttttaag catggccgcc
     1561 ggatcgggca acttgccggc ctgggccagc aaccggcaca tttcctccag cgcgtccttc
     1621 ttggtgcgcg cggcgaggtc caccgaaatc gcctggggcg atagaatatc gatcaatttc
     1681 ataaggggcg cgtccttggt cggggccggg cggctcagag attcggctcc aagaggccgt
     1741 aggtcccgtc tttgcgtcga tagatcactt tcagcgtttc actctcgtcg aagaaaagca
     1801 aaaaatcgaa ttcgctgtcg cccaactcct gggcggccgc ggccacggag aggtatttgg
     1861 gggtgacgcg gctgatgcgg gtgagcaccg gctcccgg
//
LOCUS       DAOQLS010000432         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42779_length_1898_cov_3.120456, whole genome shotgun sequence.
ACCESSION   DAOQLS010000432 DAOQLS010000000
VERSION     DAOQLS010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42779_length_1898_cov_3.120456"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PK362_08115"
     CDS             complement(<1..792)
                     /locus_tag="PK362_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC74829.1"
                     /translation="MPSVMALSDSLLATLNPPQHQAVTHGEGPLLILAGAGSGKTRVI
                     TFRIAHLLSEGVNPWNILAVTFTNKAAAEMRRRVDELTGGRGRAVWISTFHSFCAQFL
                     RVEAAAAGLDPRFTIYDDNDQTQVLKECLRELSLDEKKYKPNQVLSVISRAKDDLLDA
                     GSYTIHALAQNDPFRNMVATIYSLYQKKLAKANALDFGDLILRSTMALRDREDLRAKY
                     QSRFRHVLVDEYQDTNHAQYLLTKHLVAPPKNLCVVGDDDQSIYGW"
     gene            complement(884..>1898)
                     /locus_tag="PK362_08120"
     CDS             complement(884..>1898)
                     /locus_tag="PK362_08120"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNC74830.1"
                     /translation="GSGPAGLFWALTFALSPLVHEILRVDFHEVGVGAGLTVGLLAAW
                     EIRRPKMFWWFLVLGLLIREDYGFYLSGFGLALALARTQTPWAPPRRLAALGLGWSSL
                     LVFAVMPFLAGGVWKPAAFAFGGGVHSAADLAGRFLTLAPWREWLGNPGRLGAVLQIL
                     AGVGALPLLSPLRAVAWILPILLNAVSANPWQQRFELHYSAAFLPYLFWGGAHGLRKV
                     IEWARRPPLWKRRALPLAGLLLLGFNASRIPLYYHAASAARRAAARRMIDRLPPGDSV
                     MAQTNLLAHVSLRRFVAVAPSPRPARWIFLDNLCVDQAIPELREVQAAFLERHRSYVV
                     DED"
BASE COUNT          419 a          573 c          624 g          282 t
ORIGIN      
        1 ccagccgtag atgctctggt cgtcgtcgcc cacgacgcag aggtttttgg gcggcgccac
       61 caagtgtttc gtcaacaaat actgggcgtg gttggtgtcc tggtattcgt ccaccaaaac
      121 gtgtcggaaa cgggattggt atttcgcccg caggtcctcc cggtcgcgca gggccatggt
      181 ggagcgaaga atcaggtcgc cgaaatccag ggcgttggcc ttggccaatt tcttttggta
      241 aagggaataa atcgtcgcca ccatgttgcg gaaggggtcg ttttgggcca gggcgtggat
      301 cgtgtaggag ccggcgtcca gcaggtcgtc cttggcccgg gagatgaccg agaggacctg
      361 gttcggtttg tatttttttt cgtccagact caactcccgc aggcactcct tgagaacctg
      421 ggtttgatcg ttgtcgtcgt aaatcgtgaa gcgggggtcc aggcccgccg cggcggcctc
      481 cacccggagg aattgggcgc agaaggaatg gaaggtggat atccagacgg cgcgcccccg
      541 gccgcccgtc agttcgtcca cccggcggcg catttccgcc gcggccttgt tggtgaaggt
      601 gacggccaaa atgttccagg gattgacgcc ttcggacagg agatgggcga tgcgaaaagt
      661 gatgacccgg gttttgcccg agccggcccc ggccaagatg aggagcggcc cctccccgtg
      721 ggtcacggcc tggtgctggg gaggattgag cgtggcgagg agggaatcgg aaagcgccat
      781 aacggaaggc attatacctt aaggacgata aaatgccgaa ccgggccccc cggcgttatt
      841 ccgacggatt cggaacgttg ggaaaatgca aaaggaccaa cccctagtcc tcgtccacca
      901 cgtaggaccg gtggcgctcc agaaaggccg cctgcacctc ccgaagttcc ggaatggcct
      961 gatcaacgca aaggttgtcc agaaaaatcc accgggccgg acgggggctc ggggcgacgg
     1021 cgacgaaccg ccgcaaggac acgtgggcca acaaattcgt ttgggccatg acggaatccc
     1081 cgggcggcag gcggtcgatc atgcgccggg ccgccgcccg acgcgcggcc gaggctgcgt
     1141 ggtaataaag gggaatacgc gaagcgttga accccagcaa aaggagcccg gccagcggga
     1201 gggccctccg cttccagaga gggggacgac gggcccattc gatcactttt ctcagcccat
     1261 gggcgccccc ccaaaacaga tagggaagaa aagcggcgga atagtgcaat tcaaaccgct
     1321 gctgccaggg attggcggac acggcgttga ggaggatcgg aagaatccag gccacggccc
     1381 gaaggggact gagaagagga agggccccca cgccagcgag gatttgaagg acggcgccga
     1441 ggcgacccgg attccccagc cattcccgcc acggggcgag cgtgagaaaa cgcccggcca
     1501 aatccgcggc cgaatggacg ccgcccccga aagcgaaggc cgcgggtttc cacaccccgc
     1561 cggccagaaa cggcatgacg gcaaaaacca gcaaggacga ccaacccaac cccagggccg
     1621 ccagtcgacg gggcggcgcc cagggagttt gcgtccgggc caaggccagg gccaggccga
     1681 accccgacag atagaacccg taatcctccc gaatcaaaag ccccagaacc aggaaccacc
     1741 aaaacatttt gggtcggcgg atttcccagg cggccagaag tcccaccgtc agcccggccc
     1801 ccacgccgac ttcgtgaaaa tccacccgca ggatttcatg aaccaggggc gaaagggcga
     1861 aggtcagggc ccaaaaaaga ccggcgggac cgctcccc
//
LOCUS       DAOQLS010000433         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42956_length_1894_cov_3.258293, whole genome shotgun sequence.
ACCESSION   DAOQLS010000433 DAOQLS010000000
VERSION     DAOQLS010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42956_length_1894_cov_3.258293"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..755
                     /locus_tag="PK362_08125"
     CDS             183..755
                     /locus_tag="PK362_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNC74831.1"
                     /translation="MADDRSASASIEITAPPEVVYALITDVGRMGEWLLINTYVPQGF
                     EPDSPKFENKLKFYAGLKKLFAKHVRPDAPALWMGDLNVAPTEIDLWDPKRNAEHVCF
                     HPKARAALAAACDGLWTDLFREKEKGPDHYTYWDYLIPTNFSRNRGWRIDHMMGTPPA
                     VRRLKNIWIDRAPRGWEKPSDHTFLVAEFD"
     gene            781..1305
                     /locus_tag="PK362_08130"
     CDS             781..1305
                     /locus_tag="PK362_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNC74832.1"
                     /translation="MILRCDLHLNGGHRKLILSPHADETVDHLALKLSAFLLLWGEEP
                     QVDPSAKHPALLGQEFRPDLLALNERGEARLWIECGKVSLNKLSKVSRRFPYAQIIVV
                     KESPEDGRRLRRDLAQESLRRTVDIWTWPAEAFREWRNALGERIEVFGESGVRSLNLV
                     INETPIAVDLQVVE"
     gene            1409..>1894
                     /locus_tag="PK362_08135"
     CDS             1409..>1894
                     /locus_tag="PK362_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNC74833.1"
                     /translation="MSQPSPESLAARRGPKERRPGPDTLSKIGNVIAVGSGKGGVGKS
                     TVTANLAVALTKLGARVGILDADIYGPSQPRLLGAPLDRPPAQDGQITPTVRHGIPLM
                     SIGFIVPEGSPVIWRAPMALKALAQFLGNVEWGELDYLLIDMPQVAGEADEMNQADGF
                     GA"
BASE COUNT          356 a          620 c          587 g          331 t
ORIGIN      
        1 gacgctggtt cgggtgtagc aggaccacag cgcgccgtcg tcagcaccgt ttgtgcgatc
       61 gtgcaccgat ccgaacggtg caatcctgtc caaacggtga agcttgcctc gccctgtcgc
      121 cgagacgatg gcggcgaacc tcggcaggac gctcgcgctc ctgtggccct aggctcgctg
      181 ccatggcaga cgaccgcagc gccagtgcca gcatcgagat cacagccccg ccggaggtcg
      241 tgtatgcgct gatcaccgac gtgggccgga tgggcgaatg gttgttgatc aatacctacg
      301 tcccccaggg gttcgagccg gattccccga aattcgaaaa caaactgaaa ttctacgcgg
      361 gcttgaaaaa gcttttcgcc aagcacgtcc ggcccgacgc gcccgcgttg tggatgggcg
      421 atttaaacgt ggcgccgacg gaaatcgatc tgtgggaccc gaaacggaac gcggaacacg
      481 tttgtttcca tcccaaggcc cgggcggccc tggccgccgc ctgcgacggt ctgtggacgg
      541 atctttttcg ggaaaaggaa aagggcccgg accactacac ctactgggac tatctgatcc
      601 cgaccaattt ctcccgcaat cgagggtggc gcatcgacca catgatgggc accccgccgg
      661 cggttcggcg gctcaagaac atctggatcg accgggcgcc ccgggggtgg gaaaaaccct
      721 ccgaccacac cttcctggtc gccgaattcg actgaccgcg ccgtcggcgc cgttccgccc
      781 gtgattttac gctgcgacct tcatctcaac ggcggccacc gcaagctgat cctctccccc
      841 cacgccgatg agaccgtgga ccacctggcc cttaaattgt cggccttcct cctgctctgg
      901 ggcgaggaac cccaggtgga tccctcggcg aagcatcccg ccctgttggg ccaggaattt
      961 cgcccggatt tgctggccct gaacgagcgg ggcgaagcgc gtctctggat cgagtgcggg
     1021 aaggtgtccc tcaacaaact ttccaaggtg tcccgccggt ttccctacgc tcaaatcatc
     1081 gtggtcaagg agtctccgga ggacggtcgc cggcttcgcc gggatttggc ccaggaatcc
     1141 ctccgccgga ccgtggacat ctggacctgg cccgcggagg cctttcgaga gtggcgaaac
     1201 gccctgggcg agcgaatcga ggtttttggg gagagcggcg tccggtcctt aaacctcgtc
     1261 atcaacgaaa ccccgatcgc ggtggatctc caagtcgtgg aataggggcg aaaccgaatc
     1321 ggccggcgcg gaggaaagcc ccggcctccc tttcgcgcgt cggggccgtg tctgggaaaa
     1381 tcccgacgag aatggtaaaa tatcgattat gagccaaccc tcaccggaat ccctcgccgc
     1441 gcgacgcggc cccaaagagc gacgccccgg ccccgacacg ctgtccaaga tcggcaacgt
     1501 catcgccgtg ggaagcggca agggcggcgt ggggaaatcc accgtgaccg ccaatttggc
     1561 ggtggccctc acaaagctcg gcgcccgggt gggaatcctg gacgcggaca tctacgggcc
     1621 cagtcaaccc cgtttgctgg gcgcgcctct ggaccgtccc ccggcccaag acggccagat
     1681 cacccccacc gtccgccacg gcattcccct catgtccatc ggcttcatcg tgccggaagg
     1741 ctcgccggtc atctggcggg cgcccatggc gctcaaggcc ctggcccaat ttttggggaa
     1801 cgtggaatgg ggcgaattgg attacctttt gatcgacatg ccccaggtcg caggggaagc
     1861 cgatgaaatg aaccaggccg atggctttgg tgcg
//
LOCUS       DAOQLS010000434         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_42985_length_1893_cov_4.049510, whole genome shotgun sequence.
ACCESSION   DAOQLS010000434 DAOQLS010000000
VERSION     DAOQLS010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42985_length_1893_cov_4.049510"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PK362_08140"
     CDS             <1..496
                     /locus_tag="PK362_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74834.1"
                     /translation="AAEMPPPAEPEAPGSSNFVKGEDEQVVAVPSNPGDIPVVFFFPT
                     GKEKELGQMKKKFEDIIRKHKLKFSLFPALEVPYPMDSSLSYELFSTKCKRAGVMIAV
                     VLGPSPDGTLGEALFFHRLQELFEQQELSLQLIPWDELAKDYRFLNLALDITLLRIKQ
                     KGKP"
     gene            602..678
                     /locus_tag="PK362_08145"
     tRNA            602..678
                     /locus_tag="PK362_08145"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:636..638,aa:Cys,seq:gca)
     gene            689..1099
                     /gene="queF"
                     /locus_tag="PK362_08150"
     CDS             689..1099
                     /gene="queF"
                     /locus_tag="PK362_08150"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNC74835.1"
                     /translation="MSHKSRAYTTTHARSGVGDQLPDIECWENQFPGYTIELVFPEFT
                     SVCPKTGLPDFGTLKIRYAPGRWCLETKSLKMYLNAFRDLGIFTENVVNRVLASVVKC
                     AKPRWAVVEGRFSARGGIEARISATHGKVPREGL"
     gene            1183..1530
                     /locus_tag="PK362_08155"
     CDS             1183..1530
                     /locus_tag="PK362_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74836.1"
                     /translation="MMGAGMKCGMGVPSNHGVGGVALYLATLGLGYWLLRLAEQENKK
                     VLKNVGRFVSVVILVVSLFGLLCRAGAGICRRSCDSKKAAMAHCPFPGSMGADAHEEG
                     ASESDGEAAAPKN"
     gene            1567..1640
                     /locus_tag="PK362_08160"
     tRNA            1567..1640
                     /locus_tag="PK362_08160"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1601..1603,aa:Val,seq:gac)
BASE COUNT          364 a          512 c          597 g          420 t
ORIGIN      
        1 ggccgccgaa atgcctcccc cggcggaacc ggaagcgccg gggtcgtcca atttcgtcaa
       61 aggcgaggac gagcaggtgg tggccgtccc ttcgaacccg ggggacattc cggtcgtttt
      121 cttcttcccg acgggcaagg aaaaagaact ggggcagatg aaaaagaagt ttgaggacat
      181 catccgcaag cacaagctca aattttcgct ctttccggcc ttggaagttc cctaccccat
      241 ggacagttct ttgtcttacg agcttttttc caccaaatgc aaacgggccg gggtgatgat
      301 cgccgtggtc ctcggaccct cgccggacgg aacgttgggg gaggccctgt tttttcaccg
      361 gctccaggaa ttgtttgagc agcaggagct gtctctccag ttaatcccct gggacgaatt
      421 ggccaaggac taccgtttcc tgaacctcgc cctcgatatc acgcttctcc gaatcaaaca
      481 aaaaggcaaa ccgtaatccc gcgggcttct ccccggagga ttccctttcc cggtcggccg
      541 ggcccgcttg cgggggggac cgggaacttc gtatcatagg attgttacgg atgccccttt
      601 tggtgcggta cccaagtggc ctaaggggga agtctgcaaa acttctattc gccggttcaa
      661 atccggcccg cacctccatt atctcaacgt gagccataag tcgcgagcct acaccaccac
      721 ccacgcgcgt tccggcgtgg gagatcagtt gccggacatc gaatgctggg agaatcaatt
      781 tcccggctac acgatcgagt tggtgtttcc ggagtttacg tcggtttgtc ccaagacggg
      841 ccttccggat ttcggcaccc tgaaaattcg ctacgcgccg ggccgttggt gtttggaaac
      901 caaatcgttg aaaatgtatt tgaacgcttt tcgggatttg ggtattttta cggaaaacgt
      961 ggtcaatcgg gtattggcct cggtggtgaa atgcgcaaag ccccgttggg cggtcgtcga
     1021 agggcggttt tccgcccggg gcggcatcga agcgcgcatt tcggcgacgc acgggaaggt
     1081 tccccgggag ggattatgaa aaaaataatc agcgcattgg gtttggcgtc ggtttctttc
     1141 tcgtcggttt tcgcgaactg cggaccggag catggtatgg gcatgatggg cgccggcatg
     1201 aaatgcggca tgggcgttcc ttcaaatcac ggcgtgggcg gcgtggcgct ctatctggcg
     1261 accctggggt tgggttattg gcttttgcgt ctggccgaac aggaaaataa aaaagttttg
     1321 aagaacgtgg ggcggtttgt cagcgtcgtc atcttggtcg tcagtctttt cggactgctc
     1381 tgccgcgccg gggcggggat ttgccgccga agttgcgatt ccaagaaggc ggcgatggcc
     1441 cattgcccgt tccccggatc catgggagcc gacgcgcatg aagagggcgc gtccgagtcg
     1501 gacggagagg ccgccgctcc caagaactaa tccgacgccc tttggggcgt tgcgcattca
     1561 aatttcgctc ggttagctca gttggttaga gtacctcctt gacatggagg gggtcacagg
     1621 ttcgagtcct gtaccgagca gatttgcggg acccccaccg ggggtccggc aaatctggtg
     1681 ggttccaagg cgagaacctc ccgcggttta agcggaggtt cgcggttgga gcggaggacg
     1741 aagtcgtccg cttcattggg cggagtcctc tcaaccaagg ccaggggccg ttgtcctgaa
     1801 ccgatttgcg ggacccccac cgggggtccg gcaaaccggg tgggttccaa ggcgagaacc
     1861 tcccgcggtt taagcggagg ttcgcggttg gag
//
LOCUS       DAOQLS010000435         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_43026_length_1892_cov_3.654328, whole genome shotgun sequence.
ACCESSION   DAOQLS010000435 DAOQLS010000000
VERSION     DAOQLS010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43026_length_1892_cov_3.654328"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PK362_08165"
     CDS             <1..1389
                     /locus_tag="PK362_08165"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNC74837.1"
                     /translation="VDGMGGFLTVVAGMAALFFGVGYFLLHRSKKTAESGEAVSENQT
                     RWSWPAGANVALPAFSAFLPFLLLAAAAHRLRPADPSSLFGLGLLLLILLLGLVRRGG
                     KSLEILAPVALGCVALLEYAWHGSSFTVDRSLPALYWYGGFFALFLLLPFVAPGSFGA
                     LSAPWWAAALSGPVHGLLFYRTALALGYAPVIGALPAGGAVIYTVGLMGFLKRVPAED
                     PRRKDLLAVLGAVALLFVSLIFPLQFDKQWITLGWALEGVALLWFYRRVSHPGLKGWA
                     LALLAIAFVRLAANPAVLHYHPRAASPLFNWYLYAYGVAAACQFIAARLWSPPDQKWS
                     EVSLPGLLNALGVALLFLLMNIEIADFFSTGEALAFNLQGSLSQDLAYTLGWGGFGLA
                     LLLVGLSKESAAARWASFALLGVTIGKLFLHDVWRLTLLYRAAAFVGLAIVLILGSFL
                     YQRHFQSRGGRS"
     gene            1386..>1892
                     /locus_tag="PK362_08170"
     CDS             1386..>1892
                     /locus_tag="PK362_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74838.1"
                     /translation="MKRSLKICLCLASLGTVPLGALVREAPTAWSHFQEISSNARGLV
                     AVNLPLETLDRAASGLADLRVLSPAGGEVAYTLDAPLRTGLEWSTWPAQGAAEIRLAK
                     GRTTITASARRAAPLESIQLDISADDFLKAATLELRRDGTWRTVLAGRPVYRRRYLAE
                     DLNLVFPKQ"
BASE COUNT          229 a          621 c          605 g          437 t
ORIGIN      
        1 gtggacggca tgggcgggtt tttaacggtc gtggccggga tggcggcgct ctttttcggc
       61 gtcggttact ttctccttca ccgctcgaaa aaaaccgccg agagcggcga ggcggtttcg
      121 gaaaatcaaa cgcggtggtc ctggcccgcg ggggccaacg tggccttgcc ggccttttcg
      181 gcttttttgc ctttccttct tctggccgcg gccgcccatc gtctgcggcc ggcggacccc
      241 tcgtcgcttt ttggcctcgg cctgttgctc ctgattctcc tcctgggcct tgtgcgccgg
      301 ggggggaaat ccctggaaat cctggccccc gtcgccctgg gatgcgtggc gctcctggaa
      361 tacgcctggc acgggtcgtc ctttaccgtc gaccggtcgt tgcccgcgct ttattggtac
      421 gggggtttct tcgccttgtt tctcctcctg ccctttgtcg ccccgggttc cttcggcgcc
      481 ttgagcgctc cctggtgggc ggcggccctt tcggggcccg tccacggcct tctcttttac
      541 cggacggccc tggcgctggg ttacgcgccg gtcatcggcg ccctgcccgc cggaggggcg
      601 gtgatctaca ccgtcgggtt gatgggattt ttgaaacgcg tgcccgccga ggatccccgc
      661 cggaaggacc tgctggcggt cctgggcgcc gtggcgcttc ttttcgtcag tctcatcttt
      721 cccctgcagt tcgacaagca atggatcacg ctggggtggg cgctggaggg ggtggccctt
      781 ctttggttct accgccgggt gtcgcacccg ggattgaaag gatgggccct ggcgcttttg
      841 gccatcgcct ttgtccggtt ggcggccaat ccggcggtcc ttcactacca tccccgggcg
      901 gcatcgccct tgttcaactg gtacctctac gcctacgggg tggcggcggc ctgccagttc
      961 atcgcggcgc gcctctggag tccccccgat caaaaatgga gcgaggtttc cctgcccggc
     1021 cttttgaacg ccctgggagt ggccctcctg ttccttctca tgaatattga aatcgccgac
     1081 ttcttcagca ccggcgaagc cttggccttt aatcttcagg gcagcctttc ccaggacctg
     1141 gcctacaccc tgggttgggg cggcttcggc ctggccctct tgttggtcgg cctgtccaag
     1201 gaatccgcgg cggcccgctg ggcgagtttc gccctgctcg gcgtcaccat cggaaagctt
     1261 ttcctccacg atgtctggcg gctcacgctc ctttaccggg cggcggcctt cgtgggcctg
     1321 gccatcgtcc tgattctggg gtctttcctc taccaacggc attttcaatc ccgcggaggc
     1381 cgatcatgaa gcgatcgctt aagatttgtt tatgcttggc gtctctgggc acggttcccc
     1441 tgggggccct ggttcgggaa gccccgaccg cctggtccca ctttcaggaa atttcgtcga
     1501 acgcccgggg attggtcgcg gtgaatctgc ccctggaaac cctggaccgg gccgcgagcg
     1561 gtttggcgga cctccgcgtc ctgtcgcccg ccggcggcga agtggcctac accctggacg
     1621 cccctcttcg aaccggtttg gagtggtcca cgtggccggc ccagggagcc gccgagatcc
     1681 gcctggcgaa gggccgcacg acgattacgg cgtcggcccg ccgcgccgcc ccgctggaat
     1741 caatccaact ggacatttcc gcggacgact ttttaaaggc cgcgactctg gagcttcggc
     1801 gggacgggac ctggcgaacg gtgttggcgg gccgcccggt gtaccgccgt cggtatttgg
     1861 cggaggattt aaatctggtc tttccgaaac ag
//
LOCUS       DAOQLS010000436         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_43027_length_1892_cov_3.614589, whole genome shotgun sequence.
ACCESSION   DAOQLS010000436 DAOQLS010000000
VERSION     DAOQLS010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43027_length_1892_cov_3.614589"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="PK362_08175"
     CDS             <1..1554
                     /locus_tag="PK362_08175"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNC74839.1"
                     /translation="RWTRPQVGAALLGLALGLFGLQALVARWQWERDRVVELAVDGEE
                     VRSLSDADDDRTHGALADLADAGVSAVAIYWDPSYALPGVLREWEARVPEDLTVTLRP
                     EASPFSDWRHRWPRAARLNRVSFPVVTHALFSGPSVLGFPDMGPVQAWISSTSLTLPW
                     LEFSRQRGTSGLLAAFPERVIRAHTLSEEEMAQAGPDRVVARYLRAVRERGARFLYVR
                     LFPGLSSDDNLVFVRRLADALKEEGFELGRARPRYADFSPPLWRWPSPVRQFLAFLLA
                     VAGPFLALRWVCERPATWESPAQLTGVSLAIAFSIAALFSTPDFTLGFVGFRGVKLAL
                     LLPLIAALFYFYRAREIRHFLQEPVNVERLVWGGILIGCVGYYVLRSGHGTSVDAGGT
                     ELQIRGWLERALGVRPRFKEFFIGHPALLLGFYLRARFPKGAEVFPDRPGPWAQALHF
                     IFHDARPFLMVGFVGQLSIVNTFCHAHTPLGVSALRTFHGLWLGGLFGAAVLGAFRWA
                     HARWSARPA"
     gene            1533..>1892
                     /locus_tag="PK362_08180"
     CDS             1533..>1892
                     /locus_tag="PK362_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNC74840.1"
                     /translation="MVRPPRLTPPARALLVGYFGHGNAGDEWILRALLDALGDRRCAV
                     VVGPRPGNLGDAAAVPRTRPLRLWRSLRSCGAVVLGGGELFQNRTSLRSLLYYLFFPL
                     WARACGRPVAAVGVSVDP"
BASE COUNT          194 a          658 c          633 g          407 t
ORIGIN      
        1 cggtggaccc ggccccaggt gggcgcggcc ctcttggggt tggctctcgg ccttttcgga
       61 ttgcaggcct tggtcgcccg ttggcaatgg gagcgggacc gggtggtcga gttggcggtc
      121 gacggcgagg aagttcgctc gctgtccgac gccgacgacg accgcaccca cggggccctg
      181 gcggatttgg ccgacgccgg cgtctccgcc gtcgcgatct attgggaccc ttcctacgcc
      241 cttccgggcg tgttgcggga atgggaagcc cgggtgccgg aggatttgac cgtcactctt
      301 cgcccggaag cttcgccttt ttcggattgg cgtcaccggt ggccccgggc ggcccgcctc
      361 aaccgggtgt cctttcccgt cgtcacccac gcgctttttt cgggcccctc ggtgttgggg
      421 tttcccgaca tggggcccgt tcaggcgtgg atttcctcca cgtccctcac cttgccctgg
      481 ttggaattct cccggcaacg cggcacctcc ggtttgctgg ccgccttccc ggaacgcgtg
      541 attcgagccc acaccttgag cgaagaggaa atggcccagg cggggcccga ccgcgtcgtg
      601 gcccgctacc tgcgggcggt gcgggagcgc ggcgcgcgtt tcctctacgt ccggcttttt
      661 ccgggtcttt cctccgacga caacctggtt ttcgtgcgcc gcctggccga cgccctcaag
      721 gaagaggggt ttgagctggg acgcgcccgc ccccgttacg cggatttttc gccgccgctg
      781 tggcgttggc cctccccggt acgccagttc ctggcttttc tcctggccgt ggcggggcct
      841 tttctcgccc tgcgttgggt gtgcgagcgg cccgcgacct gggaatcgcc cgcccaactc
      901 accggcgtct ccctggcgat cgccttttca atcgcggccc tgttttccac gcccgacttc
      961 accctggggt ttgtggggtt tcggggggtg aagctggcgc tgctgcttcc cctgatcgcg
     1021 gccctgtttt atttttaccg cgcccgggaa atccggcatt tcctccagga accggtcaac
     1081 gtggaacgcc tcgtgtgggg ggggattttg atcggctgcg tggggtatta cgttctgcgg
     1141 agcgggcacg gaacctccgt ggacgcgggc ggcacggagc tgcaaattcg gggatggctg
     1201 gagcgtgcgc tgggcgtccg cccgcggttc aaggaatttt tcatcggcca cccggcgctc
     1261 ctgctggggt tctacctccg cgcccggttt cccaaaggcg ccgaggtttt ccccgaccgg
     1321 cccggcccct gggcccaggc cctgcatttt atttttcacg acgcgcggcc ctttttgatg
     1381 gtcgggttcg tgggtcaatt gtccatcgtc aacaccttct gccacgccca cacgccgttg
     1441 ggcgtttccg ccctgcgcac gttccacggg ctttggttgg gcggtttgtt cggggccgcg
     1501 gtcctcggcg cgttccgctg ggcccacgcg cgatggtccg cccgccccgc ttaacgccgc
     1561 ccgcccgcgc gttgctcgtc ggatatttcg gccacggcaa cgccggggac gaatggatcc
     1621 ttcgcgccct tcttgacgcc ctgggggacc ggcgctgcgc ggtcgtggtc ggtccccgcc
     1681 ccgggaatct gggggacgcc gcggcggtcc cccggacgcg cccccttcga ttgtggcgat
     1741 ccctccggtc ctgcggcgcg gtggtgctcg ggggcgggga actgtttcaa aaccgcacca
     1801 gcctccgaag cctcctttac tacctcttct ttcccctctg ggcgcgcgcg tgcggccggc
     1861 ccgtcgcggc cgtcggcgtc tcggtggatc cc
//
LOCUS       DAOQLS010000437         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_43440_length_1882_cov_4.530378, whole genome shotgun sequence.
ACCESSION   DAOQLS010000437 DAOQLS010000000
VERSION     DAOQLS010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43440_length_1882_cov_4.530378"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..594
                     /locus_tag="PK362_08185"
     CDS             274..594
                     /locus_tag="PK362_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNC74841.1"
                     /translation="MTSKTYHTGTTVDQVAPGTLRVFRVRGKRIAVSQVEGSFYAIDD
                     VCTHDDGPLGEGTLKGDEVECPRHGARFSVRTGAALSMPAVVPVHVYRVRVDGKNIQV
                     EWPQ"
     gene            591..1850
                     /locus_tag="PK362_08190"
     CDS             591..1850
                     /locus_tag="PK362_08190"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNC74842.1"
                     /translation="MKTPVALDIARLRLDFPILSRKINGHPLVYLDNAATTQKPMSVI
                     MSMTAYYERTNANVHRGVHTLSQEATSLMEDSRVKVAKFINAPDPATVIFTRNATESI
                     NLVAYSWARKNLKPGDEILLTEMEHHSNLVPWQLAAQATGAVLKFIPVTGWDGYLDLS
                     KISDLLTTKTKLLALTHMSNVLGTLNPVEDLARKAHAVGAKVLVDGAQSAPHLPIDVQ
                     KLGADFFVFSAHKMLGPTGLGVLWASRELLESMDPFMGGGDMIAQVWKDHSTWNELPY
                     KFEAGTPNITGAIAFGAAVDYLTKVGMDTIRAHEMDLTGYALKALKARFPKIVLHGPS
                     DVAQRGGVVSFELPGIHPHDVGTIVDREGVAIRAGHHCCQVLMKKFGVSGTARASFYI
                     YNTREEVDALVKSLEKVEELFAQKVRA"
BASE COUNT          360 a          627 c          562 g          333 t
ORIGIN      
        1 aggacgtgct ccagcgcatc cccgtccccg gcatgtcgga ccgcctgcgg gcgatcctgg
       61 acgccaaatt ggaagttccg cgtcatgcct aaatattttt ccgaaacaga tcgacgaaat
      121 tcaacgcatt ttgttctcct tccccccttg cgggggaagg cggggatggg gggtgtcttg
      181 ggtcgatccc ggtcgattca aaccgccccc cacctcgctc ctccccctcg aggggggagg
      241 atgaaaagcg tcaacctcga aggccggttt taaatgacct ctaagactta tcacacaggc
      301 acgacggtgg accaggtggc ccccggcacg cttcgggtgt tccgggtgcg gggcaagcgc
      361 atcgccgtga gccaggtgga gggttccttt tacgccatcg acgacgtctg cacccacgac
      421 gacggccccc tgggcgaagg cactttgaag ggcgacgagg tggagtgccc ccgccacggc
      481 gcgcggttca gcgtgcgcac cggcgcggcg ctctccatgc cggcggtggt gcccgtccac
      541 gtctaccgcg tgcgggtgga cggcaaaaac attcaagtgg agtggcccca gtgaaaaccc
      601 ccgtcgcctt ggacatcgcc cggctccgcc tggatttccc cattttgtcc cggaaaatca
      661 acggccaccc cctggtttac ctggacaatg ccgccacgac ccaaaaaccc atgtcggtca
      721 tcatgtccat gacggcctat tacgagcgta ccaacgccaa cgtccaccgg ggcgtgcaca
      781 ccctgtccca ggaagccacg tccctcatgg aagactcccg ggtgaaagtg gccaaattca
      841 tcaacgctcc ggacccggcg accgtgatct tcacccggaa cgccacggag tccatcaacc
      901 tggtggccta ttcctgggcc cggaagaacc tcaaacccgg cgacgaaatt ttgctgaccg
      961 aaatggagca ccactcgaac ctcgtgccct ggcagttggc ggcccaggcc acgggcgcgg
     1021 tgctcaaatt catccccgtg acgggctggg acgggtatct ggacctgtcc aagatctcgg
     1081 acttgctcac gaccaagacc aaacttctcg ctttgaccca tatgtccaac gtcctgggca
     1141 ccctgaaccc cgtggaggat ttggcccgga aggcccacgc cgtgggggcc aaggtgttgg
     1201 tggacggcgc ccagtcggcg ccccacttgc ccatcgacgt gcaaaaactc ggagcggact
     1261 ttttcgtttt ttccgcccac aagatgctgg ggcccacggg cctgggcgtt ctctgggctt
     1321 cccgggaact gttggaatcc atggacccct ttatgggcgg gggggacatg atcgcccagg
     1381 tgtggaagga ccactccacc tggaacgaac ttccctacaa atttgaagcg ggcacgccca
     1441 acatcaccgg cgccatcgcc ttcggcgcgg cggtggacta tttgaccaaa gtcggcatgg
     1501 acacgatccg cgcccacgaa atggacctca cgggctacgc cctgaaggcc ctcaaagccc
     1561 ggttccccaa gatcgtcctc cacggcccgt cggacgtggc ccagcggggg ggcgtggtga
     1621 gcttcgaact ccccggcatc cacccccacg acgtgggcac catcgtggac cgggaaggcg
     1681 tggccatccg cgccgggcac cactgttgcc aggtgctcat gaaaaaattc ggcgtgtcgg
     1741 gcacggcccg ggccagcttt tacatctaca acacccggga ggaagtggac gccctggtga
     1801 aatccctgga aaaggtggag gagctcttcg cccagaaggt ccgggcgtga gacggttcct
     1861 gttcgccgcg gccgccctct tc
//
LOCUS       DAOQLS010000438         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_43613_length_1878_cov_3.861218, whole genome shotgun sequence.
ACCESSION   DAOQLS010000438 DAOQLS010000000
VERSION     DAOQLS010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43613_length_1878_cov_3.861218"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..736)
                     /gene="metW"
                     /locus_tag="PK362_08195"
     CDS             complement(131..736)
                     /gene="metW"
                     /locus_tag="PK362_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNC74843.1"
                     /translation="MRGGRPDHAAVLAMVTPRSRVLDLGCGTGDLLALLVREKQVMAQ
                     GIELRDESIYQCVEKGLTVLHGDIEGGLGEFPDQSFDFVILNQSMQETRNVEFVMKEA
                     LRVGKAAIIGFPNMCYWKARFDVFFRGRTPVSRALPYRWHNTPNVHFLSFLDFTDFCR
                     EKGLEIMDRAALNARGPVRCWPNLFGEIAVYKIRPGQFCTL"
     gene            complement(733..1875)
                     /locus_tag="PK362_08200"
     CDS             complement(733..1875)
                     /locus_tag="PK362_08200"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNC74844.1"
                     /translation="MSAPEGVGLVETKFFTFGSLQEPMRLSSGQTLGPVTVAYETYGR
                     LDDQKTNAILLLHALTGDAHVAGFHEGEPKPGWWEAMVGPGRAFDTDKYFIVCSNVIG
                     GCRGSTGPGSVNPATGKPHALTFPVITVADMVEAQRRLMDHLGVPAWLSVVGGSMGGM
                     QALQWAASYPERVRSVVPIATTLKHSPQQIAFDEVGRQAIMADPDWMGGNYYEHGQPE
                     RGLAVARMIGHITYMSDQSMEEKFSRRLKTEGFKFTFDAEFEVEGYLRYRGAHFVRRF
                     DANSYLYVTRAMDYFDLSGDKLFKPGAPLPRFLVISFKSDWLYPSYQSRDIVSSLRSR
                     QADVTYVEVSSTYGHDAFLLEFEEQHRLLKSFLAKTRDGLAARGRS"
BASE COUNT          355 a          575 c          588 g          360 t
ORIGIN      
        1 ccctgttcct ttccagcttt cgctggtcag cggccggggc aagttggttc ttaaagcctc
       61 gccgttgcta tttctttatt ctagcaaatt caatgccgcc gcgtcaaggc tttatttatc
      121 gacggaaagt tcacaaggtg cagaattgtc cgggccgtat cttgtaaacc gcgatttccc
      181 cgaagaggtt gggccaacag cgaacgggcc ctcgggcgtt caaggcggcc cggtccatga
      241 tctcgaggcc cttttcacgg cagaaatcgg taaagtccag aaaactcagg aagtggacgt
      301 tgggcgtgtt gtgccaacgg tagggcaggg cccgggacac cggcgtccgg ccccggaaaa
      361 acacgtcgaa ccgggctttc caatagcaca tgttcgggaa gccgatgatg gccgcttttc
      421 ccacccgcag agcttctttc ataacgaatt ccacgttccg ggtttcctgc atgctctggt
      481 ttaaaatcac gaaatcgaag gattgatcgg ggaattcccc caacccgccc tcgatgtccc
      541 cgtgcaggac cgtgaggccc ttttcgacgc actggtaaat tgattcgtcc cgcagttcga
      601 tcccctgggc catcacttgt ttttcccgaa cgagcagggc gagaaggtcc cccgtcccgc
      661 acccgaggtc caagacccgg gaccggggcg tcaccatcgc cagaacggcc gcgtggtccg
      721 gtcgcccccc cctcacgacc gccccctggc ggccaagccg tcccgggtct tcgccaaaaa
      781 ggattttaaa agtcgatgct gctcctcaaa ctccaacagg aaagcgtcgt ggccgtaggt
      841 ggagctgact tcgacgtagg tcacgtcggc ctggcgggac cgcagggacg aaacaatgtc
      901 ccgggactgg taggaggggt agagccagtc ggatttaaaa gaaatgacca ggaaacgggg
      961 gagcggggcc ccgggtttga acagtttatc gcccgagagg tcaaagtagt ccatggcccg
     1021 ggtgacgtag agatagctgt tggcgtcgaa gcgccggacg aagtgggcgc cccggtagcg
     1081 aagatacccc tccacctcga actcggcgtc gaaggtgaat ttgaacccct cggttttgag
     1141 ccttctggaa aacttttcct ccatggattg atcggacatg taggtgatgt ggccgatcat
     1201 gcgggccacg gccaggcccc gctcgggttg gccgtgttcg tagtagtttc cccccatcca
     1261 gtccgggtcg gccatgatgg cctggcggcc cacctcgtcg aaggcgatct gctggggcga
     1321 gtgtttgagg gtcgtggcga tgggcaccac gctccgcacc cgctccggat agctcgcggc
     1381 ccactgaagg gcctgcatgc cccccatgga cccgcccacg accgacagcc aggcgggcac
     1441 gcccaggtgg tccatgagcc gccgttgggc ctcgaccatg tcggccacag tgatgaccgg
     1501 gaacgtcaaa gcgtggggtt tgcccgtggc cgggttcacc gacccgggcc ccgtggaacc
     1561 ccggcacccg ccgatgacgt tggagcagac gatgaagtat ttgtcggtgt cgaaggcccg
     1621 cccggggccc accatggcct cccaccagcc gggtttgggt tccccctcgt ggaagccggc
     1681 cacgtgggcg tcgccggtca gggcgtggag caggaggatg gcgttggttt tttgatcgtc
     1741 caaccggccg taggtttcgt aggccacggt gaccggcccc agggtttggc ccgaggagag
     1801 ccgcatcggc tcctgaaggg agccgaaggt gaaaaacttc gtttcaacca acccgacgcc
     1861 ctcgggcgcc gacaggtt
//
LOCUS       DAOQLS010000439         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_43739_length_1876_cov_2.707853, whole genome shotgun sequence.
ACCESSION   DAOQLS010000439 DAOQLS010000000
VERSION     DAOQLS010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43739_length_1876_cov_2.707853"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PK362_08205"
     CDS             complement(<1..485)
                     /locus_tag="PK362_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74845.1"
                     /translation="MREKRERVFTTFQIAEMCSVRPTTVIKWANQQRIKAYTTPGGHR
                     RVLESDFLEFLKKYNLPIPLELGAGRRRILVVEDEADLGQLLVRSLQRASKELDVRWT
                     RDGVEALLDIGKTPPDLVLLDVAMPLVDGTRVLATLRSDPKTQGVKVIGMTGKRLTNE
                     RL"
     gene            complement(893..1498)
                     /locus_tag="PK362_08210"
     CDS             complement(893..1498)
                     /locus_tag="PK362_08210"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC74846.1"
                     /translation="MRTFQTPDAAQNTVSHNMKSISNLSVERSLSLIRPLASISRVNA
                     ESKILTIGPRTEGEILNLLSFGFRRSNIKALDLISYSPWVLLGDMHQMPFSDDEFDVC
                     LSGWVLAYSDNKKRAAEEMVRVSRNGAIVAIGVAYTPLTKQEIVNKVGYLPGSEERMR
                     SLKDVLKWFGPHVGTIFFQQDVDEKNENTEFNLIVIFSIKK"
     gene            complement(1627..>1876)
                     /locus_tag="PK362_08215"
     CDS             complement(1627..>1876)
                     /locus_tag="PK362_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74847.1"
                     /translation="SSSHEVQSQLRVQSFAGGILFQSGFALPPKGVHHLTWRPTAAVR
                     EGNDLFCFYEFSYDRVINHRKPIVFRRFKDGSVSANHT"
BASE COUNT          447 a          491 c          522 g          416 t
ORIGIN      
        1 agacgttcgt tggtcaggcg tttgccggtc atcccgatga ccttgacgcc ctgggttttg
       61 ggatccgacc ggagggtggc caatacgcgg gtcccgtcca ccaacggcat ggcgacgtcc
      121 aacagaacga gatcgggagg ggttttgccg atgtccagga gggcttcgac gccgtcgcgg
      181 gtccaacgaa catccaattc cttggaagcg cgttgaaggg agcggaccag caattggccc
      241 aaatccgcct cgtcctccac caccaggatc cggcggcggc ccgcgcccaa ttccagagga
      301 atgggcaggt tgtacttctt gagaaactcg aggaaatccg attccaacac tcgccgatgc
      361 ccccctgggg tggtgtaggc tttgatccgt tgctgattgg cccacttaat gaccgtcgtg
      421 ggacggacgc tgcacatctc ggcgatttga aaagtcgtga acacccgctc ccgcttctcc
      481 cgcatcggaa gcccccaaaa aatccgtagt gtaattagta caacttatac aacttaagct
      541 tcccctcgaa aaataagtat agtccgacct cccccccctg tcaaggcggg tcggccaaag
      601 tgttgcaaga tcgtttcagt ggttttgcat gctgctcccc attaagccag ggaagggaaa
      661 tacgtcttga acgaatttgc tgtttttgaa tttgtctttg ggggggaccg tgacgagagt
      721 gcttaacttg aaaactcagc gcttggactc gtccgtctcg atttcggagg aggacttcaa
      781 accggccctg gggcgcactg attcttaagg cttgggaacc gagcgcctca tccggaggtc
      841 gcaaatgacg acctcgtaat cacgaccttc ggtgccaatg ggaagtcgac actcacttct
      901 ttatggaaaa aatgacgatg aggttgaatt ccgtattttc attcttttca tccacgtcct
      961 gttggaagaa aatggtccca acgtggggac cgaaccattt gaggacatcc ttaagactgc
     1021 gcattcgttc ctcggacccc ggcaagtaac ccactttatt gacgatttct tgcttcgtca
     1081 aaggcgtgta agcaacgccg atggccacga tggcgccatt ccgggagacc cggaccatct
     1141 cctcggccgc ccgttttttg ttgtcgctgt aagcgagcac ccatcccgag aggcaaacgt
     1201 caaattcatc gtcactgaat ggcatctggt gcatgtcgcc caacaggacc caagggctgt
     1261 agctgataag gtcgagggcc ttgatgtttg atcttctgaa tccaaaagac aataaattga
     1321 ggatttcccc ttcggtacgg gggccgatgg tgagaatttt actctccgcg ttaacacggg
     1381 aaatcgaggc caaaggccga atcaaagaca gcgaacgctc aaccgagagg ttcgaaatgc
     1441 tcttcatatt gtggctaacg gtgttctggg cggcgtccgg ggtttgaaag gtccgcattt
     1501 ttcgtttgag aatgacgtaa taaaagaaac gagcaaacgc cacgtaatgc cggatcacac
     1561 gaacgcggag aaattcgcgc cagcaattgg gccgtggaat gaaatttcga aagcgaatcg
     1621 tggcgctcat gtgtggttgg cgcttacgga accgtccttg aaccgtcgga acacgatggg
     1681 cttgcggtga ttgatgacgc ggtcatagga aaattcgtag aaacaaaaaa gatcgttccc
     1741 ttcacgaacc gcggccgtgg gacgccaagt taagtggtga acccctttgg ggggaagggc
     1801 aaaccccgat tgaaatagaa tccccccggc aaaggactgg acgcgaagct ggctctgaac
     1861 ctcgtgggaa gaggag
//
LOCUS       DAOQLS010000440         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_43759_length_1875_cov_4.512637, whole genome shotgun sequence.
ACCESSION   DAOQLS010000440 DAOQLS010000000
VERSION     DAOQLS010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43759_length_1875_cov_4.512637"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PK362_08220"
     CDS             <1..360
                     /locus_tag="PK362_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74848.1"
                     /translation="RAATRPLKDGLVDIQKINTGQDFGYAVVAGTPFDSGQCAVLVNS
                     FQDAAWGYQALNTASVQVLNQQPGDTLFDLKVDIRSLYTTYINDAAIVAANCNLAATL
                     GATPEAVPAPASAGNCL"
     gene            370..1698
                     /locus_tag="PK362_08225"
     CDS             370..1698
                     /locus_tag="PK362_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74849.1"
                     /translation="MKARLPMKKLSLSMALMAAALSAAAVEQPTQIRGARALGMGDAF
                     TAVADDQNVFFYNPAGSVQRTGSLATLLDIPVTLSEDFVRFGKFVSDNETDLKNFTSL
                     PPARQAELANKINTTLVKLRPTFGLGLPNTSYLSGPTANHWHWGWGLFAQASGRLGFN
                     PSVLVPSLYYDINADIVPMLNIAKGWDGALFLPGRLGLGVNVKYLRRGSASDPNVSVL
                     QLDNYNSPPLQMGSGLGLDAGLLYRPTDRWNVGVTLMDAGGTQLDFDSLDAKDGFAAK
                     PARVSGIRQRVNVGLAWTPARLGIGPIGIPTGDRLLLAVDFRDIANADSKTFFDGGVI
                     ADSAGKHIHLGAEYRWWFLRLRGGANQGYSTVGGGLDLPLLKLDYAYYSDEVGAFAGN
                     LKHSAHTVSLALRFGTGSTEGRERLKHKPAEKASDQTTPAPAADEQAKPE"
BASE COUNT          334 a          638 c          562 g          341 t
ORIGIN      
        1 cgggcggcga cgcgccccct gaaggacggg ttggtggaca ttcaaaagat caacaccggc
       61 caggattttg gctatgccgt ggtcgcagga accccgttcg actccggaca atgcgctgta
      121 ttggtcaatt cctttcagga cgccgcatgg gggtatcagg ccctaaacac cgcctcggtt
      181 caagtcttga accaacagcc gggcgatacg ctctttgatt taaaggtaga catacgatct
      241 ctatatacaa cctatattaa cgatgcggcg atagtcgcgg ctaactgcaa tttagctgcc
      301 actttaggtg caacccccga ggcagtcccg gccccggcct ccgccggcaa ctgcctctag
      361 gcccctccca tgaaagcgag actccccatg aaaaagctct ccctgtcgat ggcgttgatg
      421 gccgcggcgc tctccgccgc cgcggtggaa cagccgacgc agatccgcgg cgcccgggcc
      481 ctgggcatgg gcgacgcctt cacggcggtg gccgacgacc aaaacgtctt tttctacaac
      541 ccggccggtt ccgtccaacg gacggggtcc ctggcgacgc ttctggacat tcccgtcacc
      601 ttgagcgagg atttcgttcg cttcggaaaa ttcgtgagcg acaacgaaac ggacctgaag
      661 aattttacga gcctgcctcc cgcccgccag gcggaattgg ccaataaaat caacacgacc
      721 ctcgtaaagc tgcggccgac cttcggattg ggccttccca acacgagtta tctgtcgggt
      781 cccaccgcca accactggca ttggggctgg ggtctgttcg cccaagccag cgggcgcctg
      841 gggttcaatc cctcggtcct ggtgccctcc ctctattacg acatcaacgc ggacatcgtc
      901 cccatgctca acatcgccaa gggctgggac ggggcgctgt tccttcccgg ccgcctgggc
      961 ctgggcgtca acgtgaaata cctgcgccgc ggctcggctt cggaccccaa cgtgtcggtt
     1021 cttcagttgg acaattacaa ctccccgccc cttcaaatgg gaagcgggtt gggtctggac
     1081 gcgggcctgc tctaccgtcc cacggaccgc tggaacgtcg gcgtgacctt gatggacgcg
     1141 ggcggcaccc agttggattt tgactccctg gacgccaagg acggctttgc cgcgaagccg
     1201 gcccgggtgt ccggcatccg ccaacgcgtg aacgtgggct tggcctggac ccccgcgcgc
     1261 ctcggcatcg gccccatcgg tattccgacg ggcgaccgtt tgctcctggc cgtggatttc
     1321 cgggacatcg ccaacgccga cagcaaaacc ttcttcgacg gcggcgtgat cgccgacagc
     1381 gccgggaagc acattcacct cggcgccgaa taccgctggt ggttcctccg cctccggggc
     1441 ggcgccaacc agggctattc gaccgtcggc ggcggtctgg acctgccgct cctgaagctc
     1501 gactacgcct attactccga cgaagtcggc gccttcgcgg gcaatttgaa acactcggcc
     1561 cacaccgtgt cgctggccct tcggttcggc accggctcca ccgaaggccg ggagcggctg
     1621 aagcacaagc cggcggaaaa ggcgtcggac caaacgaccc cggcccccgc cgccgacgag
     1681 caggccaagc ctgaataaga tctcggatca ccgcttagcg cgaaaagaat cctcggcctt
     1741 cgggctgagg attctttttt tgtgcgcttt ggcgggcctc gcggtgagcg ccggcgccgt
     1801 gccgcccctc ccgggcggcc atcgacccgg ggaccggtcc acctgccggg tgaacggcga
     1861 agcccttggg cggcg
//
LOCUS       DAOQLS010000441         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_44074_length_1867_cov_5.273731, whole genome shotgun sequence.
ACCESSION   DAOQLS010000441 DAOQLS010000000
VERSION     DAOQLS010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44074_length_1867_cov_5.273731"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1867)
                     /locus_tag="PK362_08230"
     CDS             complement(<1..>1867)
                     /locus_tag="PK362_08230"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNC74850.1"
                     /translation="KNDIKDPKLKGKSALEIVMTVLHAGGKFEQNAYKYSGGLHGVGI
                     SCVNALSDKFKVEVYRDGKVYSQTYKCGKPDQDVRVTGKTENRGTRVTFHPDPTIFSE
                     HTYSFDTLAARLRELAFLNPGVRINLLDERDDKQHLFHFEGGIVEFIKFVNAHKTPLH
                     ATPIYFRKERENIQVEAAIQYTDAYTEQVYSFANNISTVDGGTHMTGFRSAITRVMND
                     YVKKHDLLKGKDYTITGDDTREGLTAVLSVRIPTQDLKFDSQTKGKLVNPEAEGLVKS
                     IVSDTLATFFEENPNTAKNICEKAILSAEAREAARKARELTRRKGALDSASLPGKLAD
                     CQERDPAKSEIYIVEGDSAGGSAKQGRNRAYQAILPLKGKILNVEKSRLAKMLANDEI
                     RTLITALGCGVGQVEGDDGINIAKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMKPL
                     VDRGYIYIAQPPLYKIKKAKKEMYIDTEDKMDEWLLAEGMDTAEVLNLSKGKSGVKVD
                     GAKLKGAFKALTELETLRKRLQKKGVSWADYLAFSKKETFPLYRVEEESGPKYLFTEK
                     EVKAWREAFIETRKAKLQQQLAAAGEAATGSAGVEEEDLSAHMKELLELKKISALA"
BASE COUNT          286 a          507 c          595 g          479 t
ORIGIN      
        1 ggccagggcg ctgattttct tgagctccaa cagttccttc atgtgggcgc tcaagtcctc
       61 ttcctccacc ccggcggacc cggtggcggc ctcgccggcg gcggccaatt gctgttgaag
      121 tttggctttg cgcgtttcga tgaacgcctc tcgccaggct tttacttcct tctcggtaaa
      181 caggtatttc gggccggatt cctcctccac gcggtagagc gggaaagttt cttttttgct
      241 gaaggccagg tagtcggccc aggaaacgcc ctttttctgg aggcgcttgc gcagggtttc
      301 caattcggtc agggccttga aggcgccttt gagtttcgcg ccgtccacct tgaccccgga
      361 tttccccttg gacaaattga ggacttcggc cgtgtccatg ccctcggcca acagccattc
      421 gtccattttg tcctcggtgt cgatgtacat ttccttcttg gcctttttga tcttgtagag
      481 gggcggctgg gcgatgtaga tgtacccccg gtccacgagg ggtttcatct gacggaagaa
      541 gaaggtgagc aacagcgtgc ggatgtgggc gccgtcgaca tcggcgtcgg tcatgatgat
      601 gatcttgtgg taacggagct tggcgatgtt gatgccgtcg tcgccttcca cctggcccac
      661 gccgcagccc agggccgtga tcaaggttcg gatctcatcg ttggccaaca tcttggccag
      721 ccgggatttt tcaacgttta agattttgcc tttgaggggc aaaatggcct ggtaagcgcg
      781 gttccgtccc tgcttggccg aaccgccggc cgagtcgcct tcgacgatgt aaatttcgga
      841 tttggccggg tcccgctcct ggcagtcggc caatttcccg ggcagggagg cggagtccaa
      901 ggcccctttg cgtcgggtga gttcccgggc cttgcgggcc gcttcccggg cctcggcgga
      961 gagaatcgcc ttttcgcaaa tgtttttggc ggtgttcggg ttttcctcga agaaggtggc
     1021 cagggtgtcg ctcacgatgg acttcacgag cccttcggct tcggggttga ccagcttgcc
     1081 cttggtctga ctgtcgaatt tgaggtcctg ggtcggtatg cgcaccgaca ggacggccgt
     1141 caaaccctcc cgggtgtcgt cgcccgtgat ggtgtaatcc ttccccttga gcaggtcgtg
     1201 tttcttcacg tagtcgttca tcacccgggt gatggccgag cggaagccgg tcatgtgggt
     1261 gccgccgtcc acggtggaga tgttgttggc gaaggagtag acctgttcgg tgtaggcgtc
     1321 cgtgtattgg atggccgcct cgacctggat gttttcccgt tccttgcgga agtagatggg
     1381 cgtggcgtgg agcggggttt tgtgggcgtt gacgaatttg atgaactcga cgatgccgcc
     1441 ttcgaagtgg aaaagatgtt gtttgtcgtc ccgttcgtcc aacaggttga tgcggacgcc
     1501 ggggtttaaa aacgccaatt cccgcaaccg ggccgccagg gtgtcgaagg agtaggtgtg
     1561 ctcgctgaaa atcgtcgggt cggggtggaa ggtcacccgg gtgccgcggt tttcggtttt
     1621 gcccgtgacg cggacgtcct ggtccggctt gccgcatttg taggtttgcg aatacacctt
     1681 gccgtcgcgg tagacttcca ccttgaattt atcggacaag gcgttgacgc atgaaatgcc
     1741 gacgccgtgg agtccgccgg aatatttgta cgcgttctgc tcgaatttgc cgccggcgtg
     1801 caggaccgtc atgacgattt ccagggccga cttgcccttg agtttcgggt ccttgatgtc
     1861 gtttttg
//
LOCUS       DAOQLS010000442         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_44117_length_1866_cov_4.587521, whole genome shotgun sequence.
ACCESSION   DAOQLS010000442 DAOQLS010000000
VERSION     DAOQLS010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44117_length_1866_cov_4.587521"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1866
                     /gene="pnp"
                     /locus_tag="PK362_08235"
     CDS             <1..>1866
                     /gene="pnp"
                     /locus_tag="PK362_08235"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNC74851.1"
                     /translation="DNEILTSRLIDRSIRPLFPEYLRLEVQITALALSHDGLHDTDIL
                     TILGASVALGMSSLPWNGPIGAVRIARIDERFVFNPTLDEQDRSVLDLVVAGKKGSIL
                     MVESGSYELSEELLLEALALAQQEIDKICDMQTELFRKIGKPKMVVAAPAIDAGLTAK
                     VNELARAKFAEAMRIAEKTARETFIDGLVKQVKDALATDYPEQGGDISALLATIEYEE
                     ARRLILDEKRRTDGRGFEDVRPISIQSGVLPRVHGSALFTRGQTQALATATLGSPADQ
                     QIMDELEGEYKERFMLHYNFPGFSTGEPKAERGPGRREVGHGALAKRALAPLLPDAEQ
                     FPYTLRVVSDILESNGSSSMATVCGGSLALFDAGVPMKRAVAGIAMGLVKEGERTAVL
                     TDIMGMEDHLGDMDFKVAGTTEGVTALQMDIKIEGISVAIMKQALEQAKRARLFVLGK
                     MSQALAEPRPDLAPTAPRMEVVKIPVDKIGALIGPGGKNIRRIITESGAQVEVEDDGS
                     VYITAIDRASMETAKRQIEGYSAEAEVGKIYKGTVVRIMPKLGAFIEIFPGKDGLCHI
                     SQLDVTRVERVEDVVKVGDQLEVKVLEIDNMGRVNLSRRAVIKPGSELEVAPRGPR"
BASE COUNT          384 a          633 c          535 g          314 t
ORIGIN      
        1 gacaacgaaa ttttgaccag ccggttgatc gaccgctcca ttcggccgct gtttcccgaa
       61 tacctccggt tggaagtcca aatcaccgcc ctggcccttt cccacgacgg cctgcacgac
      121 accgacattc tgacgattct gggcgcctcc gtggccctgg ggatgtcctc cctgccgtgg
      181 aacggcccca tcggcgccgt ccgcatcgcc cgcatcgacg aacgtttcgt gttcaacccc
      241 accttggacg aacaagaccg cagcgtgttg gacttggtcg tggccggcaa aaaaggctcc
      301 atcttgatgg tggaatccgg cagctacgaa ctttccgaag agctcctcct ggaagcgctg
      361 gccctggccc agcaggaaat cgacaaaatc tgcgacatgc agaccgagtt gttccgcaaa
      421 atcgggaaac ccaaaatggt cgtggccgcg ccggccatcg acgccggcct gaccgcgaaa
      481 gtcaacgagt tggcccgggc caaattcgcc gaagccatgc ggatcgccga gaaaaccgcc
      541 cgcgaaacct ttatcgacgg cctcgtgaaa caggtcaagg acgccctggc caccgattac
      601 cccgagcagg gcggcgacat ttccgcgctt ttggcgacca tcgagtacga ggaagcccgt
      661 cgcctcatct tggacgagaa gcgccgcacc gacggccgtg gtttcgaaga cgtccgtccc
      721 atttcgattc aaagcggcgt tttgccccgg gttcacgggt ccgctctctt cacccgcggc
      781 caaacccaag ccctggccac cgccaccctg ggatccccgg cggaccaaca gatcatggac
      841 gagctcgaag gggaatacaa agagcgcttt atgctccatt acaattttcc cgggttttcg
      901 accggcgaac cgaaggccga acgcggcccc ggccgccggg aagtgggcca cggcgccctg
      961 gccaagcgcg ccctggcgcc gctcctgccc gacgccgagc aattccctta caccctccgg
     1021 gtcgtttccg atattttgga atccaacggc tcctcctcca tggccaccgt ctgcggcggc
     1081 tccctggcgc tctttgacgc cggcgtgccg atgaaacgcg ccgtggccgg catcgccatg
     1141 gggttggtga aggaaggcga acgcaccgcg gtcctgaccg acatcatggg catggaagac
     1201 catttgggcg acatggattt caaagtcgcc ggcaccaccg agggcgtcac ggcgcttcaa
     1261 atggacatta agattgaggg catttccgtc gccatcatga agcaggctct cgagcaggcc
     1321 aaacgggccc ggttgttcgt tctcggcaaa atgtcccagg ccctggcgga accccgcccc
     1381 gacctggcgc cgaccgcgcc ccgcatggaa gtggtcaaga tccccgtgga caagatcggc
     1441 gccttgatcg gccccggcgg caaaaacatc cgccgcatca tcaccgagtc cggcgcccag
     1501 gtggaagtgg aagacgacgg ttccgtctac atcaccgcca tcgaccgcgc ctccatggaa
     1561 accgccaaac gccagatcga aggctacagc gccgaagccg aagtgggcaa gatttacaaa
     1621 ggcaccgtcg tccgcatcat gccgaaactg ggcgccttca tcgaaatctt ccccggcaag
     1681 gacggcctct gccacatctc ccaactggac gtcacccgcg tggaacgcgt ggaagacgtc
     1741 gtcaaagtcg gcgaccaatt ggaagtcaag gtcttggaaa tcgacaacat gggccgcgtc
     1801 aacctttccc gccgggcggt catcaaaccc ggctcggaat tggaagtcgc gccccggggt
     1861 ccgcgt
//
LOCUS       DAOQLS010000443         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_44420_length_1859_cov_3.802661, whole genome shotgun sequence.
ACCESSION   DAOQLS010000443 DAOQLS010000000
VERSION     DAOQLS010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44420_length_1859_cov_3.802661"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PK362_08240"
     CDS             complement(<1..455)
                     /locus_tag="PK362_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74852.1"
                     /translation="MNFPLRKSAAPLAALFLLGAATARGEVRPGEFSAGLLSSGVAFQ
                     GMALSRLSLGVHYQKQRGVGVYGLRQRWYANPKAPTINVFAGLEENRVTFDIDGVRGR
                     GWTVGAYVGLDYFVARRWSLEMDAGPAFIDLRDGDNAVTNEGLDFVVTLG"
     gene            599..1360
                     /locus_tag="PK362_08245"
     CDS             599..1360
                     /locus_tag="PK362_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74853.1"
                     /translation="MRRSSTVQRAGAFGLVLFAGIAAGAKEKGEPDFLAKALETAGGT
                     PKFDRIETLQGRFFLVRPSTSGIVNIAVDFAARRTPEGWRVAEKIPGAAARVWTASAP
                     FDLGDPAGDPDFRSARERFFLLLAPYQLEQFRLPARYLGMGFLQNRLQRRLALGPAGG
                     AAEAWTIYVDTAPAFLRGMDARAAPGTWLFEGEEMFQNQFFLPTRWTRYSAEGKRLEV
                     VRVGDLVFNALLNPDLTIAPDPAVGGAAPKQGETP"
     gene            1357..>1859
                     /locus_tag="PK362_08250"
     CDS             1357..>1859
                     /locus_tag="PK362_08250"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNC74854.1"
                     /translation="MMEERLICRRSEAADARFTLKGSESHYAPDRTFDTEHIRLDLSL
                     DIAKKTLRGTASTKLRALAGGADRMVFDAVHFKIESVRSGGRALAHEYDDKKLTVRFP
                     KPFAAGAVVTVDIAYRVTKPKLGLYFIGPDRGYPRKPVQVWTQGEDEYARYWFPCHDS
                     PQDRVTT"
BASE COUNT          343 a          614 c          582 g          320 t
ORIGIN      
        1 cccagggtaa cgacaaaatc caggccttcg ttggtgaccg cgttgtcgcc gtcccttaaa
       61 tcgatgaagg ccgggcccgc gtccatttcc agggaccagc gtctcgcgac gaaataatcc
      121 agacccacgt aggcccccac cgtccatccc cgcccccgga ccccgtcgat gtcgaaggtg
      181 actcggtttt cctccaggcc cgcgaaaaca tttatcgtcg gagctttcgg gttcgcgtac
      241 catcgttggc gcagaccgta aacccccacg ccgcgctgtt tttgatagtg gacccccaaa
      301 gagagtcggg aaagggccat tccttgaaag gccacgccgg aggaaagcag gcccgccgaa
      361 aattcgccgg ggcgcacttc gccacgggcc gtcgcggcac cgaggaggaa aagggccgcc
      421 aggggcgcgg cagattttcg aagggggaaa ttcatggggt tcgggacaca ttatacccgg
      481 gattggcgta tcccggacgc gcctccaccg cccggagggg ccggcccccg gggtttccgg
      541 gccctaggga aaaaccctcc cgaagcgacg aaggcaaatt ggtatactcc gtcaataaat
      601 gagacgttca tcgaccgttc aacgcgccgg cgccttcggc ctggtcttat ttgccggaat
      661 cgccgccggg gcgaaggaaa aaggcgaacc ggattttctg gcgaaagccc tggaaacggc
      721 cgggggaacg cccaaattcg accggatcga aaccctccag gggcgcttct tcctcgtgcg
      781 cccctccacg tcgggcatcg tgaacatcgc ggtggatttc gccgcccggc gcacgcccga
      841 gggctggcgg gtggccgaga aaattccggg ggcggcggcc cgggtgtgga ccgcctcggc
      901 gccctttgac ttgggcgacc cggccgggga tccggacttt cgttccgccc gggaacgctt
      961 tttccttctt ctggccccct accaactcga acaattccgc cttccggccc gttacctggg
     1021 catgggcttt ttgcaaaacc ggctgcaacg ccgcctggcc ctgggacccg ccgggggggc
     1081 cgccgaggcc tggacgattt acgtcgacac ggcgcccgct tttctgcgcg ggatggacgc
     1141 tcgcgcggcc cccgggacct ggctgttcga gggcgaagaa atgtttcaaa atcagttctt
     1201 cctgccaacc cggtggacgc ggtattccgc cgagggcaag cgcctggaag tggtgcgggt
     1261 gggcgacctg gtgttcaacg cccttctgaa cccggatttg acgatcgcgc cggaccccgc
     1321 cgtgggcggg gccgcgccga aacaaggaga gactccatga tggaagaacg cctgatttgc
     1381 cgacggtccg aagcggccga cgcccggttc acgctcaaag ggtccgagtc ccattacgcg
     1441 ccggaccgca ccttcgacac cgaacacatt cgcttggacc tttctctcga catcgccaag
     1501 aaaacacttc ggggcacggc ctccacgaaa ctgcgggccc tggcgggggg cgcggaccgc
     1561 atggtttttg acgcggtgca cttcaaaatc gaatcggttc gttccggcgg tcgggcgctc
     1621 gcccacgaat acgacgataa aaaattgacg gtccgctttc ccaagccctt cgcggcgggc
     1681 gccgtcgtca ccgtggacat cgcctaccgc gtgaccaagc ctaagctggg cctctatttc
     1741 atcgggccgg accggggcta tccccggaaa cccgttcagg tctggaccca gggggaggac
     1801 gaatacgccc gttactggtt cccctgccac gattcgcccc aggaccgggt gacgacgga
//
LOCUS       DAOQLS010000444         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_44530_length_1857_cov_3.450610, whole genome shotgun sequence.
ACCESSION   DAOQLS010000444 DAOQLS010000000
VERSION     DAOQLS010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44530_length_1857_cov_3.450610"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PK362_08255"
     CDS             <1..759
                     /locus_tag="PK362_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC74855.1"
                     /translation="PPAVDDLFELEPLVVTAPRLKISGEQFIDARIDNELVNLLEKIA
                     TEPPPEEVIVDPALAEVNQLVTPAGFGLKIRYSDIAFLITEGLAGTTNLTLINRLETI
                     ARQSPNTRARASALVALGHDATRNDLQIFQDALKDSSRIVRFGAVEALAFQTNPMARP
                     ILAGAAQTDESAPVRLFAAQALSRAGDAQGTEILRRALSDPDWVVRALSTYLVGLLGD
                     DTDFERIMINMNRESNDRVTAENCLAILRLARAS"
     gene            756..1376
                     /locus_tag="PK362_08260"
     CDS             756..1376
                     /locus_tag="PK362_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC74856.1"
                     /translation="MNAALHVLVGTGGSWVVYGLLLASVLTVAVIVERAQVLVRERKE
                     LALWKAKIKSPLAEGDLARVTELTAKAAGVGAAILRGGLDKAGAAPAAVEERLAAARI
                     EGKYQLERRLWVLGTLGNNAPFIGLFGTVLGVIKAFGDLAAATTAGPEVVMAGLSEAL
                     IATAVGLLVAIPAVIAYNYFMKRAGEYLAETDALSRRLLAALKDHR"
     gene            1380..1790
                     /locus_tag="PK362_08265"
     CDS             1380..1790
                     /locus_tag="PK362_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC74857.1"
                     /translation="MAAGGEEREGLITGINVTPLVDVVLVLLIIFMATAPLISRRALA
                     VAVPKAATGERSTAALRVTRAADGQIFLEKTPQTLAGLMQELQTRLKLEPDLHVSVAA
                     EERLPYGDVVELLDTIRSAGVKKVALDVRPAPKK"
BASE COUNT          325 a          606 c          603 g          323 t
ORIGIN      
        1 ccccccgcgg tggacgattt gtttgagttg gaacccctgg tcgtcacggc tccccggctc
       61 aagatttccg gcgagcagtt catcgacgcc cgaatcgata acgagctcgt gaacctgttg
      121 gagaagatcg ccacggagcc cccgcccgag gaagtgatcg tggacccggc cctggccgag
      181 gtcaatcaac tggtcacgcc ggccgggttc ggactcaaaa tccgctactc ggacatcgcc
      241 tttctaatca ccgaaggcct ggcggggacg acgaacttga ccttgatcaa ccggctggaa
      301 accatcgccc ggcaaagtcc caacacccgg gcccgggcct cggccctggt ggccctgggc
      361 cacgacgcca cccgaaacga cctgcaaatt tttcaagacg ccctgaaaga ttccagccgg
      421 atcgtccgct tcggcgccgt ggaggccctc gcgttccaaa cgaaccccat ggcccggcca
      481 atcctggccg gcgccgccca gacggacgaa tcggccccgg tgcggctgtt cgcggcccag
      541 gccctttccc gggcggggga cgcccagggc acggagatcc tccgacgggc gctgtccgat
      601 cccgattggg tggtgcgggc gctttcgact tatctggtgg gattgttggg cgacgacacg
      661 gacttcgaga ggataatgat caacatgaac cgagaatcca acgaccgcgt gaccgcggaa
      721 aattgtttgg cgattttgcg cctggcgagg gcctcgtgaa cgccgccctt cacgtcctgg
      781 tgggaacggg cgggtcctgg gtcgtctacg gactgcttct ggcttcggtt ttgaccgtgg
      841 ccgttatcgt cgaacgcgcc caggtcctgg tgcgggagcg caaagagctg gccctttgga
      901 aggccaaaat caaaagcccg ttggccgagg gggatttggc ccgggtgacg gagctgacgg
      961 cgaaagcggc cggggtcggc gccgccattc tgcgcggggg cctggacaaa gccggcgcgg
     1021 cgcccgccgc ggtcgaggaa cgcttggccg ccgcgcgcat cgaaggcaaa taccaattgg
     1081 aacgacgcct ttgggtgctg ggcaccctgg gcaacaacgc cccgttcatt ggacttttcg
     1141 ggacggtgct gggcgtgatc aaggctttcg gcgatctggc cgcggccacc accgccggtc
     1201 ccgaggtcgt catggccggt ctttccgaag ccctgatcgc cacggcggtg ggccttctgg
     1261 tggccattcc ggcggtcatc gcctacaact atttcatgaa acgggccggg gaatacctcg
     1321 ccgaaaccga cgccctgtcc cgccgtcttt tggcggcgtt aaaagaccac cgataattta
     1381 tggcggcggg gggcgaggag cgggaagggc tgatcaccgg catcaacgtc acgccgttgg
     1441 tggacgtcgt cctggtcctc ctcattattt tcatggccac cgccccgctc atctcccgac
     1501 gggccctggc cgtggcggtg cccaaggccg cgaccggaga gcggtccacg gccgccctgc
     1561 gggtgacccg cgccgccgat gggcagatct tcctcgaaaa aaccccccaa accctggccg
     1621 gcctgatgca agaattgcaa acgcggctta aactcgagcc ggacctccac gtctccgtgg
     1681 cggccgagga gcgcctgcct tacggcgacg tggtggagct gttggataca atccgctccg
     1741 ccggtgtcaa aaaggtggcg ctggacgttc gccccgcgcc caagaaatag cggagggtcg
     1801 atggaccgtc ggacctggac gcctttccaa aaagggttgg ccgcttccct ggccctg
//
LOCUS       DAOQLS010000445         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_44925_length_1849_cov_3.381271, whole genome shotgun sequence.
ACCESSION   DAOQLS010000445 DAOQLS010000000
VERSION     DAOQLS010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44925_length_1849_cov_3.381271"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..917
                     /locus_tag="PK362_08270"
     CDS             312..917
                     /locus_tag="PK362_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019439681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNC74858.1"
                     /translation="MNDVRASLVLSAALVFGIGCATAPKVQRTEVDQTIDLSGDWNDT
                     DSRMTAEEMVKDSLSRPWLGEFSGAKGVKPRVIVGTVLNKSHEHINTETFVKDLEREL
                     TNSGQVKFVASKDQRDEVREERMDQAKNSSVKTAKSMGQEYGADFMLKGQINTIMDAA
                     GKQEVKYYQVELEMIDMSSNEKAWIGQKKIKKLVTYRKKKF"
     gene            939..>1849
                     /locus_tag="PK362_08275"
     CDS             939..>1849
                     /locus_tag="PK362_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74859.1"
                     /translation="MPVPRRGALPLLLLAVGCAAGLGIRELNGLVARGEFAQAAVRVE
                     GAKDSYGAKNALLFELDRAFLLQLAGDWATSNTVFEQAKRTAKDLFTKSVTAEASTFL
                     VSDNMRPYYGEDFERALIHVFCAVNYTLLGQPAEALVECRQVDFYLKTLQVDYARKNA
                     HTEDAFARYLAGLLYEDAGETNDAYISFFKALEAYDGYAKKYKTPRPEGLVVDALRTA
                     KALGFRDEMADIKNRWGDAPAVRGPQQGELIVLDYNGFAPQKMDSFFEISVTNGWVYV
                     DQLKAGSEEDAKVDEARAVLRGIAADK"
BASE COUNT          399 a          593 c          541 g          316 t
ORIGIN      
        1 ccagctcctt ttcgtcccgg ggttgccgga ccaccacgta ctgcccggag gaaagtcccc
       61 gaaaaaggcc gggggccagg acttcgcagg aaccgcaggc gatgcaggcc gtggtcgtgt
      121 agaagcgccc gggagcgttc ccctccgctt tttgcaaatc cgcctcggcc atgaaatttt
      181 ctccttgttg aaaaggttct cccccccatc cttggatttt tgaaggaccc gcgcaaggcg
      241 cgggcggggc cggtccttgc gccccgggac ttcatgaact agactttccc tatccctttt
      301 aggaggaact catgaacgac gttcgcgctt ccctcgtttt gtccgccgcc ctggtcttcg
      361 ggatcggctg cgccaccgcc cccaaggtcc aacgcaccga ggtggaccag accatcgacc
      421 tctccggtga ctggaacgac acggactccc gcatgaccgc cgaagaaatg gtgaaggatt
      481 ccctgtcccg accctggttg ggcgaatttt ccggcgccaa aggcgtcaaa ccccgcgtga
      541 tcgtgggcac cgtgctgaac aaaagccacg aacacatcaa caccgagacg ttcgtgaagg
      601 atttggagcg ggaactgacc aattccggcc aggtcaaatt cgtcgcctcc aaggaccagc
      661 gggacgaagt ccgcgaagag cgcatggatc aggccaagaa ttcctcggtc aaaaccgcca
      721 aaagcatggg ccaggaatac ggggccgact ttatgctcaa gggccagatc aacacgatca
      781 tggacgccgc gggcaagcag gaagtcaagt attaccaggt cgagttggaa atgatcgaca
      841 tgtcttccaa cgagaaggcc tggatcggcc agaagaaaat caagaaactg gtcacctacc
      901 gcaagaaaaa gttttaaccc ggaggattcg ggaggccttt gcccgtgccg cggcgagggg
      961 cgctccctct ccttctcctg gccgtcggct gcgcggccgg cctcggaatc cgtgaactga
     1021 acggcttggt cgcccggggc gagttcgccc aggcggcggt gcgggtggag ggcgccaagg
     1081 attcctacgg cgccaaaaac gccctgctct ttgaattgga ccgggccttc ctgctccagc
     1141 tcgccggcga ctgggcgacg agcaacaccg ttttcgagca ggccaagcgc accgccaagg
     1201 acctcttcac caaaagcgtc accgccgaag cctccacctt cctcgtcagc gacaacatgc
     1261 gaccctacta cggcgaggac ttcgagcggg ccttgatcca cgttttctgc gccgtcaact
     1321 acaccctttt gggccagccc gccgaggccc tggtcgaatg ccggcaagtg gatttttacc
     1381 tcaaaaccct gcaggtcgat tacgcccgaa aaaacgccca caccgaggac gccttcgccc
     1441 gctacctggc ggggctcctg tacgaggacg ccggcgaaac caacgacgcc tacatttcct
     1501 ttttcaaagc gctggaagcc tacgacgggt acgccaagaa atacaaaact ccccgccccg
     1561 aggggctggt ggtcgacgcg cttcgaaccg ccaaagccct gggcttccgg gacgaaatgg
     1621 cggacattaa gaaccgatgg ggcgacgccc ccgccgtccg ggggccccag cagggcgaac
     1681 tgatcgtcct ggactacaac ggattcgccc cgcagaaaat ggactccttt tttgagatca
     1741 gcgtcaccaa cggctgggtc tacgtggacc agctgaaggc gggcagcgag gaagacgcca
     1801 aggtggacga agcccgggcg gtcctccggg gtatcgcggc cgacaaaat
//
LOCUS       DAOQLS010000446         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_44940_length_1849_cov_2.732441, whole genome shotgun sequence.
ACCESSION   DAOQLS010000446 DAOQLS010000000
VERSION     DAOQLS010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44940_length_1849_cov_2.732441"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PK362_08280"
     CDS             <1..331
                     /locus_tag="PK362_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNC74860.1"
                     /translation="VNSLHDGMNLVAKEFVAARDDLDGVLILSTFAGASRELVEALLV
                     NPYDTERMADALRFALEMSPDEQADRMKRMREVVREHNIYRWAGELIEELTRVRLASE
                     SAPVRAL"
     gene            328..1107
                     /gene="otsB"
                     /locus_tag="PK362_08285"
     CDS             328..1107
                     /gene="otsB"
                     /locus_tag="PK362_08285"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNC74861.1"
                     /translation="MTKSFWGFARRWVGRVRAAPRVLLALDFDGTLAPLVDHPSRARM
                     PATLRKRLGALTDRCDVAVLSGRSLADVRKRVGVGKVFYGGNHGLEMSGPGFGYVNPT
                     ARRLKRHTGEMMRLAQEALRGVAGVHLEEKGFGISVHYRRVRPEQMVFFKTVLSTLKR
                     KTHARAFHWTRGHCVWELRPRVRWDKGRALRTIWRHRGRPFVIAVGDDATDEDMFRAA
                     VGRGFAVKVGPEKTRAAYRLSRQGEVLAFLKWTLELVRRIP"
     gene            1104..1766
                     /locus_tag="PK362_08290"
     CDS             1104..1766
                     /locus_tag="PK362_08290"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HNC74862.1"
                     /translation="MKKAASPFRFFTRLALTLIRSEKAHTAEELLGGLRSLPDGVIYF
                     HTHRFLHEHQFLVPEPMNDFAYWAEKIAGEPALAKGLSGVNPLRHSTLAQVRSALVAV
                     VDAHVRKNPGRAPLPAEKAFQFMSARRFSIPTPHTAANLEEMVEALGRVTHASLYLHL
                     FEANLRLPPGENDFSVWLSADWGDPSLAEKIRGLDLSLHTLEEIKNKMVAFLRSSESS
                     HA"
BASE COUNT          336 a          539 c          609 g          365 t
ORIGIN      
        1 cgtgaacagc ctgcacgacg gcatgaacct cgtcgccaag gagttcgtcg cggcgcgcga
       61 cgatctcgac ggcgtgctca tcctcagtac cttcgccggc gcctcgcggg aactcgtcga
      121 ggcgctgctc gtcaatccct acgacacgga gcgcatggcc gacgccctgc ggttcgcgct
      181 tgaaatgtcg cccgatgaac aggccgatcg aatgaaacga atgcgcgagg tggttcggga
      241 acacaacatc taccgctggg ccggggaatt gatcgaggaa ctaacccggg ttcgactggc
      301 ttcggagagc gcccccgtcc gcgccctatg acgaaatcat tttgggggtt cgcccgtcgg
      361 tgggtgggtc gcgtccgggc ggcgccgcgc gtcttgctgg ccctggattt cgacgggacg
      421 ctggcccccc tcgtggatca tccatcccgg gcgcggatgc cggcgaccct gcgaaaacgc
      481 ctgggggcat tgaccgaccg ctgcgatgtg gcggtcctca gcggacgctc gttggcggat
      541 gtgcgaaaac gcgtgggggt ggggaaggtg ttttatgggg gcaatcacgg cttggaaatg
      601 tccgggcccg ggtttgggta cgtcaacccg accgcccggc ggttgaaacg tcatacggga
      661 gaaatgatgc gtctggccca ggaggccctt cggggcgtgg cgggggtgca tttggaggaa
      721 aagggattcg ggatcagcgt gcattaccgg cgggtgcgac ccgaacaaat ggtttttttt
      781 aagaccgtcc tgtcgacgct caaaaggaaa acccacgccc gggcatttca ttggacccgg
      841 ggccattgcg tttgggagtt gcggccgcgc gtgcgctggg acaaggggcg ggccttgagg
      901 acaatttggc ggcatcgcgg ccggcccttc gtgatcgccg tgggggacga cgccaccgac
      961 gaagacatgt ttcgcgccgc ggtgggccgg ggattcgccg tgaaagtggg gcctgaaaaa
     1021 acccgcgcgg cctaccggct ctcccgtcag ggggaggtgc tcgcgtttct caaatggacc
     1081 ttggaactcg tcaggagaat cccatgaaaa aagccgcttc gccttttcgt ttttttaccc
     1141 gcttggcctt aactctgatt cgaagcgaaa aagcccacac cgccgaggag ctgctggggg
     1201 gattgcgctc gttgccggac ggcgtgattt actttcacac ccaccggttt ctgcacgaac
     1261 atcaattcct ggtccccgaa ccgatgaacg acttcgctta ttgggcggag aaaatcgccg
     1321 gggaacccgc attggcgaag ggattgtcgg gggtgaaccc cctccgccat tcgacgttgg
     1381 cccaagttcg ttcggccttg gtcgcggtgg tggacgccca cgtgcggaaa aacccggggc
     1441 gggccccgtt gccggcggaa aaggcttttc aattcatgtc cgcccgacgc ttttccatcc
     1501 cgacgccgca caccgcggcg aacctggaag aaatggtcga agcgctgggc cgcgtgaccc
     1561 acgccagcct ttaccttcat ctgtttgaag ccaacctccg cttgccgccc ggggaaaacg
     1621 atttctccgt ttggctgtcg gcggattggg gcgacccgtc cttggcggaa aagattcggg
     1681 gcctggattt atcccttcac acgctcgaag agatcaaaaa taaaatggtc gcgtttcttc
     1741 gttcgtcgga gtcgtcccat gcgtaggctt gaggaatacc ggaccgtcgt ggggtcggac
     1801 gttttggagg aattgcaatt gttggccaac cccctgtccg gaaaacggg
//
LOCUS       DAOQLS010000447         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_44981_length_1848_cov_3.198550, whole genome shotgun sequence.
ACCESSION   DAOQLS010000447 DAOQLS010000000
VERSION     DAOQLS010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44981_length_1848_cov_3.198550"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1848
                     /locus_tag="PK362_08295"
     CDS             <1..>1848
                     /locus_tag="PK362_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74863.1"
                     /translation="FSTVDISYDALGRVVKSRDLVHQGGAGLDAYTTEETTNDLFDNW
                     GRAVDSLSIVTNGALKTVSHSHGLAFDANGRLTRQTIDLHESSTLPGIELSADRTITQ
                     TDMKYDAIGNLVDYTKIAKEGDLETTNRPALLEYDKNGRSVVSLERVTDNFDRDEIVG
                     NVGSQYNELGQLIGGQSVTIKGDSKALNGKQLSGIVAAILNGDLQSVKGQTLVKSLGL
                     EGLWANRTVSPTRYDAQGRLVFTDTVMDKAGWGWNNVTETKVLFAQTIGRWDRWAYED
                     RVDAIIAQMKAQGLSVTNKTFQISGDTRRHRYCYASVTYVKQVWALQDTHERSTNAVT
                     AFDALNRPIDQTLTMLRGANTTVNRQRTGYDAQGRVTALESRLRETGINGDGSPLDRR
                     FNQRQSFTYDAAGRSSAEVTRTWGDSAAPLKDTTSTVDQIVYASGQRVAWSETTTSTE
                     RTQQNVRRMDSVQYDGLGRIAAYNADTYEGEPGALQLQYSDRDARNTYDALSRLVLTL
                     GLRNWGRSSSPLPSAASPGLSPSPLMGEGRDGGETGVPGGGIQGLSDGWTAGSVTLAI
                     AYEYANGSSSISGQWITAHGTGSNAEASVRAAGINLNHHEYGMRYDSVG"
BASE COUNT          404 a          610 c          531 g          303 t
ORIGIN      
        1 gtttctccac cgtcgatatt tcttacgacg ccctgggccg cgtggtcaaa agccgggacc
       61 tggttcacca gggaggcgcg gggttggacg cctacacgac cgaagagacc accaacgatc
      121 tttttgacaa ctggggacgc gccgtggact ccctttccat cgtgaccaac ggggcgttga
      181 aaaccgtgtc ccattcccac ggcttggctt tcgacgccaa cgggcgtctc acgcgccaga
      241 cgatcgacct tcacgagtcc tccaccctgc ccggcatcga attgtccgcc gatcgcacca
      301 tcacccagac ggacatgaaa tacgacgcca tcggcaacct ggtggactac accaagatcg
      361 ctaaggaagg ggacctggaa accacgaacc gccccgccct tttggaatac gacaagaacg
      421 ggcgctcggt tgtttccttg gagcgagtca ccgacaactt tgaccgcgac gaaatcgtcg
      481 gcaacgtggg gtcccaatac aacgaactgg gccagttgat cggcgggcaa tccgtgacga
      541 tcaaagggga ctccaaagcg ttaaacggca aacagctctc ggggatagtc gcggcgattc
      601 taaatggcga cctgcaatcc gttaagggcc agacgctcgt caaatccctg ggactggaag
      661 gcctctgggc taaccgaacg gtttccccca cccgctacga cgcccaggga cggctggtgt
      721 tcaccgatac ggtcatggac aaagcggggt ggggttggaa caacgtaacg gagacgaaag
      781 ttctcttcgc ccaaaccatc gggcgctggg atcgatgggc ctacgaggac cgtgtcgacg
      841 ccatcatcgc ccaaatgaag gctcaagggc tttcggtgac gaacaaaacg ttccagatat
      901 ccggggacac aagacgccat cgctattgct acgcgtcggt gacctacgtg aagcaagttt
      961 gggcccttca ggacacccac gagcggtcca ccaacgccgt gacggctttt gatgccttaa
     1021 accgccccat cgaccaaacc ctcacgatgc ttcggggcgc caacacgacc gtcaaccgtc
     1081 agcgaacggg ctacgacgcc cagggccgag tgaccgccct ggagagccgc ctgcgggaaa
     1141 ccggcatcaa cggcgacgga agcccgttgg accgccggtt caatcagcgg caatcgttta
     1201 cctacgacgc cgccggccgg tcttccgccg aggtgactcg aacctggggc gacagcgccg
     1261 cgccgcttaa agacacgacc tccaccgtgg atcaaattgt ttatgcaagt ggccagcgcg
     1321 tggcctggtc cgaaaccacc acttccaccg agcgaaccca acaaaacgtc cggcggatgg
     1381 attcggttca atacgacggt ctgggacgaa tcgccgccta caacgccgac acctacgaag
     1441 gcgagcccgg cgccctccaa ctccaataca gcgaccgcga cgcccgcaac acctacgacg
     1501 ccctgagccg cctcgtgctg acactcggcc tacggaattg gggtcggtca tcgagccccc
     1561 tgccaagcgc tgcctcccct gggttgtctc cttccccctt gatgggggaa ggccgggatg
     1621 ggggtgaaac cggggttccg ggcggcggca ttcagggctt gagcgacggc tggaccgccg
     1681 gatcggtcac cctggccatc gcctacgaat acgccaacgg atcctcctcc atttccggcc
     1741 aatggatcac cgcccacggc acgggatcca acgccgaagc ctcggtgcgt gccgccggga
     1801 ttaatttgaa ccaccacgaa tacggcatgc gctacgacag cgtcggcc
//
LOCUS       DAOQLS010000448         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45001_length_1847_cov_6.769531, whole genome shotgun sequence.
ACCESSION   DAOQLS010000448 DAOQLS010000000
VERSION     DAOQLS010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45001_length_1847_cov_6.769531"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..275)
                     /locus_tag="PK362_08300"
     tRNA            complement(204..275)
                     /locus_tag="PK362_08300"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(241..243),aa:Asn,seq:gtt)
     gene            437..>1847
                     /locus_tag="PK362_08305"
     CDS             437..>1847
                     /locus_tag="PK362_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC74864.1"
                     /translation="MSPFHRTVSRPVAIALFGFAGLLVVISLVLRHFLVDLPSIQSLE
                     NYTPSLVTKLYDVRGELITELFVEKRSVLPLPQIPVDFQHAVLAIEDNNFYTHWGVDA
                     KGVIRAFLANLRSGRLVQGASTVTQQLAKNIFLTRERTMTRKIKELLLTLQMEASLSK
                     DEILQLYINQIYFGNGAYGLESAAKTFFGKTADQLNLPECALLAGLPKGPDKYSPYRH
                     RDRAIRRRNLVLRRMREEGYITEQEELRAGGAGHIFTKKAPDKINAQYFVEMVRLQLE
                     PTYGSEALYKGGYSIYTTLDARMQRAAEEATQKNLTSFDDRYAEQRLTQLVKDKKLTP
                     EFLEKWKKWKADPEKNEEPEEHVEPVPVQGALVSVDPHTGGIRALVGGRDFQESQFNR
                     ATQAKRQPGSTFKPFVWLAALESGLTAATVVDDLPIAYTDVERHPRLVAEATDYATLM
                     EMVTGYYTPDLPPDAPNPIW"
BASE COUNT          392 a          608 c          526 g          321 t
ORIGIN      
        1 ctcagggagt tttttttgca ccctttgggc gcaaaaaaaa ctccccggcc tggacaacgg
       61 gccacaatac ggccctaagt ggagaggatt tttactttaa gcgtcttccg ggcggaagac
      121 gcggccaccg cgaaccttgg ctttgccaag ggttctagcc accctcaggg agtttttttt
      181 gcaccctttg ggcgcaaaaa aaactccccg gcctggactc gaaccaggaa cccttcgatt
      241 aacagtcgaa tgctccacca ttgagctacc ggggaataaa tttgtgggtg cccgaccggg
      301 ggtcggggga tttaaagaac ttgtttgaga cgggtcatat taccattctc tcccggaggc
      361 cgcaagaaac gggccagggt tttccgtcgg gtgggggcct tgaggggggc tcccgaaaaa
      421 cataggatgg tgcgtcatgt cccccttcca ccgcaccgtc tcccgccccg tggccatcgc
      481 ccttttcggc ttcgcgggcc tgttggtggt catctccctg gtgctccggc atttcctggt
      541 ggacctgccc tccattcaaa gcctggaaaa ctacaccccc tccctggtga ccaagctcta
      601 cgacgtccgg ggcgagctga tcaccgagct tttcgtcgaa aagcggagcg tcctgccgct
      661 cccccagatc cccgtggact tccagcacgc ggtgctggcc atcgaggaca acaactttta
      721 cacccattgg ggcgtggacg ccaaaggcgt catccgggcc tttctggcca acctgcgatc
      781 ggggcggctg gtccaggggg cctcgaccgt cacccagcag ttggccaaaa acattttttt
      841 gacccgggaa cgcaccatga cccggaaaat caaagaactc ctgctcaccc tccaaatgga
      901 ggccagcctc agcaaggacg agatccttca gctctacatc aaccaaatct atttcggcaa
      961 cggagcctac gggctggagt ctgccgctaa aacctttttc ggcaaaacgg ccgaccagct
     1021 aaacctgccg gaatgcgccc tcctggccgg cctgcccaag gggccggaca aatattcccc
     1081 ctaccgccac cgggaccggg ccatccgtcg gcggaacctc gtgctccgcc gcatgcggga
     1141 ggaaggctac atcaccgagc aggaagaact tcgggcgggc ggggcgggcc acattttcac
     1201 caagaaggcc ccggacaaaa tcaacgccca atatttcgtg gagatggtcc ggcttcagct
     1261 ggaacccacc tacgggtcgg aagcgctcta caagggcggc tactccattt acaccaccct
     1321 ggacgcccgc atgcaacggg cggccgagga ggccacccag aaaaacctca cctccttcga
     1381 cgaccgctac gccgagcaac ggctgaccca gctggtcaaa gacaaaaaac tgactccgga
     1441 atttttggag aagtggaaaa agtggaaggc cgacccggaa aagaacgagg agcccgagga
     1501 acacgttgag cccgtgcccg tccagggcgc cctggtttcc gtggaccccc acacgggcgg
     1561 cattcgggcc ctggtgggcg ggcgggactt ccaggaaagc cagttcaacc gggccaccca
     1621 ggccaaacgc cagccgggct cgaccttcaa acccttcgta tggctggcgg ccctggaatc
     1681 cggactcacg gcggccaccg tggtggacga cctgcccatc gcctacaccg acgtggagcg
     1741 ccacccgcgc ctggtggccg aagccacgga ctacgccacg ctgatggaga tggtcacggg
     1801 ctactacacg ccggacctgc cgcccgacgc gcccaacccc atctggg
//
LOCUS       DAOQLS010000449         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45060_length_1846_cov_4.589615, whole genome shotgun sequence.
ACCESSION   DAOQLS010000449 DAOQLS010000000
VERSION     DAOQLS010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45060_length_1846_cov_4.589615"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="PK362_08310"
     CDS             <1..1816
                     /locus_tag="PK362_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC74865.1"
                     /translation="DGPQTWSARSRWARERLSAFLRRAAGTETVWERLDSVLENLAAL
                     DLFAPPTSEEEFAETFYEAVTRVRLPGTPDGGVRFLGAMEARGERFRWLFLLGLNEGA
                     FPRTVTEDPLLSDDLRRVLRDPGGYWILPKMEGYDEEKLLFGLLAASARDGLTALYAR
                     SREDGRSEIPSLYLRELARATGHSLEAAEQIPRSPRRKWNDVAPDGLTPQEAGLADLL
                     DGRPPPVEWRSLVSRAADLAAWGAATARDGLVGRPAAWLDRLAERGLSPSAIETYVSC
                     PFEFFMGRVLRAPAASPVFDGERVSAAFLGKVQHHVLFKTHDRFRNGPLPTVEAAAAH
                     LEETVRVLFDALRRLPGGPPALIWDVLAEGVRVRLAAFIRRDIDELRATGRRPSELEG
                     PRSAPHPTAGFRWNGRLDRVDLHSATGARTVIDYKNRPRRESLSSRILKGRVHQAPAY
                     LDLLSSTPGGGEAGVRYEYLATNEFEEFTAEEWRAARTAVHENQKALFEEMARGVFPI
                     RPSEGPEGHCAFCSFSSACRKAHGPSRVRAERVVGPLGRDMAPSDKRGRPLAAASSSL
                     ESPENAERASGGGGNDPAKPPARKSKGTPGRPARGKSP"
BASE COUNT          269 a          624 c          641 g          312 t
ORIGIN      
        1 ggacgggccg caaacctggt cggcccggtc ccggtgggcc cgggaacgcc tgtcggcctt
       61 cctccgccgg gccgcgggga ccgagacggt ttgggaacgg ctggattctg ttctggaaaa
      121 cctggccgcc ctggatctat tcgcgccccc gacctcggag gaggagttcg ccgagacgtt
      181 ttacgaagcc gtgacccggg ttcgccttcc cggtacgccc gatggggggg ttcggttcct
      241 gggggccatg gaggcccggg gcgagcggtt tcgttggctc ttcctcctgg ggctcaacga
      301 aggcgcgttc ccccgaacgg tgaccgaaga cccgcttctg agcgacgatt tgcgccgggt
      361 ccttcgggac cccggcggtt attggatcct ccccaagatg gaaggctacg acgaggaaaa
      421 gctgctcttc ggtctcctgg cggcgtcggc ccgggacggc ctgacggccc tgtacgcccg
      481 ctcccgggag gatggccgat ccgagatccc ttccctctat ttgcgggaac tcgcccgggc
      541 caccggccat tccctggagg cggccgagca aattccccga tcgccccggc gcaaatggaa
      601 cgacgtcgcc cccgacgggt tgacccccca ggaagccggg ttggcggatt tgctggacgg
      661 ccggccgccg cccgtcgaat ggcgatccct cgtgtcccgg gcggcggact tggcggcctg
      721 gggcgcggcc acggcccggg acggtttggt ggggcgaccc gcggcgtggc ttgaccgtct
      781 ggcggagcgg ggattgtccc ccagcgcgat cgaaacgtac gtgtcctgcc cctttgaatt
      841 tttcatgggg cgggtcctgc gagcgccggc ggcctccccc gtcttcgacg gggaacgggt
      901 gtcggccgcg ttcctgggga aagtccaaca ccacgtcctg tttaaaaccc acgaccgctt
      961 tcgcaacggg ccccttccga cggttgaggc ggcggcggcc cacctcgaag agaccgttcg
     1021 ggttctgttc gacgccctcc gtcgtttgcc cgggggaccc ccggccttga tttgggacgt
     1081 gctggccgaa ggggttcgtg ttcgactggc cgcttttatc cgccgcgata tcgatgaatt
     1141 aagggccacc gggcgccgac cgtcggaatt ggaagggccc cgatcggcgc cccaccccac
     1201 cgcgggattt cgctggaacg gccggttgga ccgggtggac cttcactcgg ccaccggggc
     1261 gcggaccgtc atcgactaca aaaatcgacc gcggcgggag tccctctcga gccggattct
     1321 caagggccgg gtccaccagg ccccggccta tttggacttg ctgtcgtcga cgcccggcgg
     1381 gggcgaggcc ggggttcgtt acgaatacct ggccaccaac gagttcgagg aattcaccgc
     1441 cgaggaatgg cgggccgccc ggacggcggt tcacgaaaat caaaaggccc tgtttgagga
     1501 aatggcgcgg ggcgtgtttc ccattcgacc ctccgagggg cccgaaggcc actgcgcctt
     1561 ttgttccttc tcttcggcct gccggaaagc ccacggtccc tcccgggtcc gggccgagcg
     1621 ggtcgtcggc cctctgggcc gagacatggc cccttccgac aagcgggggc gacccctggc
     1681 ggcggcgtcg tcgtcgttgg agtctccgga aaatgcggag cgggcctccg gcggaggggg
     1741 gaacgacccg gccaaaccgc ccgcccggaa atccaaagga accccgggac gaccggcccg
     1801 gggaaaatcg ccgtgatcct cgaccaatcc gcccgggatt tggccc
//
LOCUS       DAOQLS010000450         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45101_length_1845_cov_3.868156, whole genome shotgun sequence.
ACCESSION   DAOQLS010000450 DAOQLS010000000
VERSION     DAOQLS010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45101_length_1845_cov_3.868156"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..>1845)
                     /locus_tag="PK362_08315"
     CDS             complement(141..>1845)
                     /locus_tag="PK362_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74866.1"
                     /translation="LLVTGAQSEEVAVECMKRGADDYILKSTLKRLPSAVLNALKKRE
                     TEHQKEKAEEDLRAQEEQYRLIAEHTRDLIYLLDAKGQFTYLSPSFQKTLGYAPEELI
                     GKKAFSLLHPDDKTAARKAFQEALESRTGRTVPYRCKMKTGAWVLFESVVSWAFDDKG
                     VPNKAVIVSRDIDERKRLEEQVRQAQKMEAVGRLAGGVAHDFNNLLTAITGYSDLLLR
                     SLDRMDPRRADVEEIKETAARAATLTQQLLAFSRRQITQPTVLDLNHVVSNLEKMLRR
                     LIGEDIQLRTVLTPHIHRVRADLGQIEQIVINLGVNSRDAMPEGGKLVIETANREIQA
                     GEEATGDIPPGHYAVLSVTDTGCGMDPKTLNHLFEPFFTTKEKGHGTGLGLSTVYGIV
                     QQSGGHIRVKSALGEGTTFWIYLPRVEDVVAPADKSSETDEFFKGNETVLLAEDDRTV
                     RTLAVRILRQSGYRVLEASSGEEALRLLEESTTAIDLLVTDVVMNGISGPELAKRVNQ
                     KLPGVKILYLSGYMESDMRNDILKGVASSFLGKPFRPRDLVQRVRETLDGASIPGGDK
                     K"
BASE COUNT          315 a          547 c          562 g          421 t
ORIGIN      
        1 accgccgtcc aaccgttcga gggcctcgtc gccggtggag gcggtggcga cgtcgtggcc
       61 ggcgcttcgc agaacgttgg aggccatgtc gcgcatgact ttttcgtcgt ccaccaccag
      121 aatttttagg gatttggagg tcattttttg tccccgcccg ggatcgacgc gccgtcgagg
      181 gtttcccgca cccgctggac caaatcccgg ggtcgaaagg gttttcccag aaaggacgac
      241 gccacccctt tgagaatgtc gttgcgcatg tccgactcca tgtaacccga taaatagagg
      301 atcttcacgc ccggcagttt ttggttgacg cgcttggcca gctccgggcc cgaaatcccg
      361 ttcatcacga cgtccgtgac caacaggtcg atggcggtgg tggattcttc caggaggcgg
      421 agggcctctt cgccgctgga agcttccagg acgcggtaac cgctttgtct caatattcgg
      481 acggccaggg tgcgaacggt tcgatcgtcc tcggccaaca ggacggtttc gttcccctta
      541 aaaaactcgt cggtttcgga cgatttgtcg gcgggggcga caacgtcctc cacccgggga
      601 aggtagatcc aaaacgtcgt tccttcgccg agggcgctct tgacccggat atgaccgccg
      661 gactgctgga cgatgccgta gaccgtggag agtcccagcc ccgtcccgtg gcccttctcc
      721 ttcgtcgtaa aaaacggttc aaacaaatgg ttaagcgttt tcgggtccat gccgcagccg
      781 gtgtcggtga cggaaagcac cgcgtaatgg cccgggggaa tgtccccggt cgcctcctcc
      841 cccgcctgaa tttcgcggtt ggcggtctca atcaccaatt tccccccctc gggcatcgcg
      901 tcgcgggagt tgacgcccag attgatgacg atttgctcga tctggcccag gtcggcccgg
      961 acccggtgga tgtggggggt caggacggtc cgcaattgaa tgtcctcgcc gatcagccgt
     1021 cgaagcattt tttccaggtt ggacaccacg tggttcaaat ccaggaccgt gggttgggtg
     1081 atttggcggc ggctgaaagc cagaagctgc tgggtcagcg tcgccgcccg ggccgccgtt
     1141 tctttgattt cctccacatc ggcccgccgg ggatccatgc ggtccaggga gcggagcaaa
     1201 agatcggaat aacccgtgat ggccgtcagg agattgttga aatcgtgggc cacgccgccg
     1261 gccagtcgcc ccaccgcctc cattttttgg gcttgccgga cctgttcctc caggcgcttt
     1321 cgttcgtcga tgtcccggga gacgatgacg gctttgttgg ggacgccttt gtcgtcaaag
     1381 gcccagctga ccaccgattc gaacaacacc caagcccccg ttttcatttt gcatcggtag
     1441 ggcaccgttc ggcccgtccg gctttccaag gcttcctgaa aggcctttcg ggccgccgtt
     1501 ttgtcgtcgg gatgcaaaag ggagaaggct tttttgccga tgagttcctc gggcgcgtag
     1561 cccagggttt tctggaagga agggctgaga taggtgaatt gacctttggc gtccagcaaa
     1621 taaatcaaat cccgggtgtg ttccgcgatc aggcggtatt gctcctcctg ggcgcggagg
     1681 tcctcctcgg ctttttcttt ttgatgttcg gtttccctct ttttcagggc gttgaggacc
     1741 gccgacggca atcgtttcag cgtcgatttg agaatgtagt cgtcggcccc gcgtttcatg
     1801 cattcaacgg cgacttcctc cgactgggcg ccggtcacga gcaga
//
LOCUS       DAOQLS010000451         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45159_length_1844_cov_3.084405, whole genome shotgun sequence.
ACCESSION   DAOQLS010000451 DAOQLS010000000
VERSION     DAOQLS010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45159_length_1844_cov_3.084405"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="PK362_08320"
     CDS             complement(<1..85)
                     /locus_tag="PK362_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HNC74867.1"
                     /translation="MRTVAVVQARMGSTRLPGKVLRPLGSRT"
     gene            complement(105..1100)
                     /gene="pseB"
                     /locus_tag="PK362_08325"
     CDS             complement(105..1100)
                     /gene="pseB"
                     /locus_tag="PK362_08325"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNC74868.1"
                     /translation="MFDDKSILITGGTGSFGRRFVKTVLTRFKPKRLIVYSRDELKQY
                     EMQQEIQSAALRYFIGDIRDGDRLTQAMRGVDLVVHAAALKQVPAAEYNPMECIKTNV
                     HGAENVIAAALDNDVEKVIALSTDKAASPVNLYGATKLVSDKLFVAANNMTGKHRTRF
                     AVVRYGNVVGSRGSVVPYFQKLIDRGDRDLPVTDARMTRFWITLDQGVAFVLAGFERM
                     RGGEIFIPKIPSVRILDLVESMRPGTKPRFIGVRPGEKIHEVMVTADDAIKTLEFDDH
                     YVIQPSIVFITPVDYRSNARGESGRPVAEGFEYNSGTNPLFLTPAEIRGLHGGAA"
     gene            complement(1125..>1844)
                     /locus_tag="PK362_08330"
     CDS             complement(1125..>1844)
                     /locus_tag="PK362_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNC74869.1"
                     /translation="VAHLARWIQSLPNRHYTYRFVFIPETIGSICYLSRNLDRLRKYV
                     VAGYNVTCVGDDRAYSFLPTPEGNTLSDEVARHVLGHTHPEYIVYENADRESDERQYG
                     SPGVDLPVCSIMRSKYRAYPEYHTSLDNLELVTPSGLFGAYDVYQKCVECLEMNPILK
                     STVVCEPQLGRRGLYPTLSDGKHYDQIKIMRNLIGYANGRRSLLAIAGKLKIPLWQIQ
                     PVARRLMDEGLLGEIRETITE"
BASE COUNT          395 a          549 c          494 g          406 t
ORIGIN      
        1 cggtccggga tcccagcggg cgcaacacct tcccgggaag gcgcgtggaa cccatccgcg
       61 cctgaacgac cgcgacggtt ctcattttcc gggacggggc cagttcacgc ggcccccccg
      121 tggagtcccc gaatttcggc cggggtcaag aacaagggat tggtccccga attgtattca
      181 aaaccctccg ccaccggccg gccggattcg ccccgggcgt tcgaccggta atcgacgggg
      241 gtgatgaaga cgatcgaggg ctggatcacg taatgatcgt cgaattccag ggtcttgatg
      301 gcgtcgtcgg cggtgaccat cacctcgtgg attttttcgc ccggccgaac gccgatgaac
      361 cgcggcttcg ttccgggacg catggattcc accagatcca aaattcgcac cgaggggatc
      421 ttgggaatga aaatttcccc cccgcgcatg cgttcgaacc cggccaggac gaacgccacc
      481 ccctgatcga gggtgatcca aaaccgcgtc atgcgggcgt cggtgacggg caggtcgcgg
      541 tccccccggt cgatcaactt ctggaaatac gggaccaccg acccccggga ccccacgacg
      601 ttcccgtagc gcacgacggc gaaccgggtg cggtgcttcc cggtcatgtt gttggcggcg
      661 acaaacagct tgtccgacac cagtttcgtc gcgccgtata aattgacggg gctggccgct
      721 ttgtccgtcg acagggcgat caccttttcc acgtcgttgt ccagggccgc cgcgatgacg
      781 ttttcggccc cgtgcacgtt ggttttgatg cactccatgg gattgtattc cgcggccggc
      841 acctgtttaa gtgccgcggc gtgcacgacc aaatccactc cccgcatggc ctgcgtcagg
      901 cggtccccgt cccgaatgtc gccgataaaa tagcgtaaag cggctgattg gatttcttgc
      961 tgcatttcat attgcttcaa ttcgtcacgt gaataaacga taagacgttt gggtttgaac
     1021 cgcgtcaaga ccgtcttcac gaaccggcgg ccaaaggatc ccgtcccgcc ggtgattaaa
     1081 atggatttat catcaaacat tttccctccg ccaatcgtga aaaattattc ggtgatcgtc
     1141 tcgcgtattt cacccaacag cccttcgtcc atcaatcgcc gggccacggg ctgaatttgc
     1201 caaagaggta ttttcagttt gcccgcaatc gccaacaaag acctcctacc gtttgcgtag
     1261 ccaatcaggt ttctcatgat tttaatttga tcgtagtgtt tcccatcgct taaggtcggg
     1321 taaaggccac gtcgtcccaa ttggggctcg cacaccaccg tcgattttaa tatgggattc
     1381 atctccagac attccacgca tttttgataa acatcgtacg ccccgaacaa ccccgagggg
     1441 gtcaccaatt ccaggttgtc caacgacgta tggtactccg gataagctcg atatttggag
     1501 cgcataatcg agcaaaccgg caagtccaca ccggggctgc cgtattgacg ctcatcgctt
     1561 tcccgatcgg cgttttcgta aacgatatat tcgggatgcg tatgccccaa aacgtggcgc
     1621 gccacttcgt cggataaagt gtttccctcc ggagtcggga ggaaagagta agcgcggtca
     1681 tccccgacgc acgttacgtt gtatccggcg acgacatatt ttcgcaaacg atccaaattt
     1741 cggctcaaat aacagatgga acctatggtt tccggaataa aaacaaaccg gtaggtgtaa
     1801 tgcctattgg gcaacgactg gatccatctc gccaaatggg caac
//
LOCUS       DAOQLS010000452         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45184_length_1843_cov_5.245526, whole genome shotgun sequence.
ACCESSION   DAOQLS010000452 DAOQLS010000000
VERSION     DAOQLS010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45184_length_1843_cov_5.245526"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK362_08335"
     CDS             complement(<1..218)
                     /locus_tag="PK362_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HNC74870.1"
                     /translation="MKVLVLGSGGREHALVWALRKSGGVSEVYCAPGNAGISGLAVVP
                     ALDVTKSKEIVHFAKEKEIGLVIIGPEGP"
     gene            complement(215..1615)
                     /gene="purF"
                     /locus_tag="PK362_08340"
     CDS             complement(215..1615)
                     /gene="purF"
                     /locus_tag="PK362_08340"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNC74871.1"
                     /translation="MNGVTRDPLDVPREECGVFGIYNHKEAARLAYLGLQQLQHRGQE
                     SAGIVTSDGNRFFAHVSMGLVADVFKPEALDRLKGRMAVGHVRYATAGSSSLANAQPI
                     VVATARGPMAIGHNGNLTNAMTLRKKMESLGSIFQTSSDTEVILHLMAKSPAREIVTA
                     LKDALSQVTGAYSLVIQTTKSLYAVRDPWGVRPLHLGRLNGSLVVASETCAFDIIGAK
                     LVREVKPGEIVAIDHRGARTVAQMPSPSKAHCVFEYVYFARPDSHIFGKSVYEVRREM
                     GRQLAREAPAKADIVVAVPDSANVAAVGYADESGIPLEIGLIRSHYRGRTFIEPKQSI
                     RDFGARMKYAAVVEALKGRRVVVIDDSIVRGTTSRKLIRMLRHSGAREIHLRISSPPI
                     MGPCFYGIDTPQARELIATRMSVEKIRQYLNTDSLKYLSLEGMLKATGRNPKEFCTAC
                     FNTRYPIPVEDAGRVA"
     gene            complement(1612..>1843)
                     /locus_tag="PK362_08345"
     CDS             complement(1612..>1843)
                     /locus_tag="PK362_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74872.1"
                     /translation="LVALESAIRKLSAAGQLSVLVGAHGQPQALIRKSQFLSTERRMI
                     RHCPDRTAAAQEAAAFLRAAGSATPLFPEARR"
BASE COUNT          307 a          565 c          626 g          345 t
ORIGIN      
        1 ggcccctcgg gcccgatgat caccaggccg atttcctttt ccttggcgaa gtggacgatt
       61 tccttgcttt tggtcacgtc cagggccgga accacggcca ggccgctgat gcccgcgttg
      121 ccgggggcgc aatagacttc cgatacgccg ccgcttttgc gcagggccca aacgagcgcg
      181 tgttcgcgcc cgcccgatcc gagaacgaga actttcacgc gaccctcccg gcgtcttcaa
      241 cgggaatggg gtagcgggtg ttgaagcagg ccgtgcaaaa ttccttcggg ttgcgacccg
      301 tggccttgag catgccctcc aaactcaaat acttgaggct gtcggtgttc agatactgac
      361 ggattttttc gacgctcatg cgggtggcga tcagctcccg ggcctggggc gtgtcgatgc
      421 cgtagaagca ggggcccatg atggggggcg agctgatccg caaatggatc tctcgggcgc
      481 cggaatggcg aagcatccgg atcaacttcc ggctggtcgt tccccgcacg atggaatcgt
      541 cgatcacgac gacccggcgg cctttgaggg cctcgaccac cgccgcgtat ttcatgcggg
      601 cgccgaaatc ccgaatggat tgtttgggct cgataaaggt gcggccccgg taatggcttc
      661 ggatcaagcc gatttccagg ggaatcccgg attcgtcggc gtagccgacg gcggccacgt
      721 tggccgagtc cggcacggcg accacgatgt cggctttggc cggggcttcc cgggccagct
      781 gccggcccat ttcccggcga acctcgtaga cgcttttccc gaaaatgtgg ctgtcgggcc
      841 gggcgaagta gacgtattcg aacacgcagt gggccttgga cgggctcggc atctgggcca
      901 cggtccgggc gccccggtgg tcgatggcga cgatttcccc gggtttcacc tcgcgcacga
      961 gcttcgcccc gatgatgtcg aaggcgcagg tttccgaggc cacgacgagg gagccgttca
     1021 accggcccag gtgaaggggt cggacgcccc aggggtcccg gacggcgtag agggatttgg
     1081 tcgtttgaat caccagcgaa taggcgccgg tcacctggct cagggcgtct ttgagggccg
     1141 tcacgatttc ccgggcgggg gatttggcca tgaggtggag aatcacttcg gtgtccgagg
     1201 aggtttggaa gatggacccc agggattcca tttttttgcg caaggtcatg gcgttggtca
     1261 aattgccgtt gtggccgatg gccatggggc cccgggccgt ggcgacgacg atgggttggg
     1321 cgttggccag ggaggaggag cccgccgtgg cgtagcgcac gtggcccacg gccatgcgcc
     1381 ctttcaggcg gtccaacgcc tcgggcttga agacgtcggc caccaggccc atggacacgt
     1441 gggcgaagaa acggttgccg tcggaggtca cgatgccggc ggactcctgc ccccggtgct
     1501 gcaactgttg aagccccaaa taggccagtc gcgcggcttc tttgtggttg tagatgccga
     1561 acacgccgca ttcttcgcgg ggcacgtcca ggggatcgcg cgtcacacca ttcatcgtct
     1621 cgcctccggg aacaaagggg tcgccgagcc ggccgcccga aggaacgccg cggcctcctg
     1681 ggccgccgcg gttcgatccg ggcagtggcg gatcatccgc cgctcggtgc ttaaaaattg
     1741 cgatttgcga atcaacgcct ggggttggcc gtgggccccc accaggaccg acaactggcc
     1801 cgccgcggac aacttccgaa tggcgctttc gagcgccacc aac
//
LOCUS       DAOQLS010000453         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45227_length_1842_cov_4.616676, whole genome shotgun sequence.
ACCESSION   DAOQLS010000453 DAOQLS010000000
VERSION     DAOQLS010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45227_length_1842_cov_4.616676"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK362_08350"
     CDS             complement(<1..231)
                     /locus_tag="PK362_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74873.1"
                     /translation="MAKTKVLLVEDDRAIAKILRYNLEKEGHQVLHAEDGEAGVALFR
                     REKPRLVVLDLMLPKVDGLEVCRLIRQTDPGVP"
     gene            complement(252..632)
                     /locus_tag="PK362_08355"
     CDS             complement(252..632)
                     /locus_tag="PK362_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74874.1"
                     /translation="MTESKGKIVIADDEEDITRIVKRMLELEGYTVWAANDGQAALDL
                     YRREKPQLVILDVSMPLKDGVTVCDEIRRDDDRVLILMLTGQKKESDKVTGLSVGADD
                     YLTKPFGEKELMARVRSLFRRPNR"
     gene            complement(640..1284)
                     /gene="recR"
                     /locus_tag="PK362_08360"
     CDS             complement(640..1284)
                     /gene="recR"
                     /locus_tag="PK362_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNC74875.1"
                     /translation="MNGTGAWDRLVSALLGMPGVGPKMAERAALHLIRRRDAGDALLL
                     ALADARARMRRCPLCGDFVEGPPLEAADAAVLPEADGPCDRCRDPHRDDRLLCVVEDV
                     GDLAALERSRAFGGKYHVLGGVLSALDGVGPEELRIEALLDRVSGGTTEEVILATNPS
                     VEGETTAAYLADLLKNRGVRVTRLASGLPSGSVIQYADEHTLAQALSGRRAVPA"
     gene            complement(1281..>1842)
                     /locus_tag="PK362_08365"
     CDS             complement(1281..>1842)
                     /locus_tag="PK362_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74876.1"
                     /translation="LRVAETPTPAPVVPLTGPDGLRAAWVKTLADVEIKKPSLAPFLS
                     SARPRFDSGGGLELACAQEFQKQQLVNQQVFWSDLLRAHAGRPVTVTLTVAAPPPAPA
                     APRPQPAPVVEEAEEVVDEEADAPPPVESPATEGEEQDPRALGALDAGLKKVLDKFPG
                     KIRKADPAESAPAAASKPRAEGKPGA"
BASE COUNT          269 a          592 c          658 g          323 t
ORIGIN      
        1 gggaacgccc gggtccgtct ggcggatcag gcggcagact tccaggccgt ccactttggg
       61 caacatgagg tccaggacga ccaagcgggg tttctcccgg cggaacaggg ccaccccggc
      121 ctcgccgtcc tccgcgtgga ggacctgatg gccctccttt tccaggttgt aacggaggat
      181 tttggcgatg gcccggtcgt cttccacgag gaggaccttg gttttggcca tgggaaggga
      241 ccgggagcgg gtcagcggtt cggtcggcgg aagagggagc ggacccgggc catcaattcc
      301 ttttccccga aaggcttggt gaggtaatcg tcggcgccga cggaaagtcc ggtcaccttg
      361 tccgattcct tcttttgccc ggtcagcatc aaaatgagga cgcggtcgtc gtcccggcgg
      421 atttcgtcgc agacggtgac gccgtccttg agcggcatgg aaacgtccag gatcacgagc
      481 tggggttttt cccgccgata gaggtccagg gcggcctggc cgtcgttggc ggcccagacg
      541 gtgtatccct ccaattccag catgcgcttg acgatgcggg tgatgtcttc ctcgtcgtcg
      601 gcgatgacga tttttccttt ggattcggtc atggggtcct cacgcgggca ccgcccgacg
      661 gccggagagc gcctgggcca gggtgtgttc gtcggcgtat tggatgacgc tgcccgaggg
      721 cagccccgag gccagtcgtg tcacccggac gccgcggttt ttaagaaggt cggccaaata
      781 ggcggccgtc gtttcgcctt ccaccgatgg gttggtggcc aaaatcactt cctcggtcgt
      841 gccgccggac acgcggtcca gcagggcttc gatgcggagc tcctcggggc ccaccccgtc
      901 cagggccgag agcaccccgc ccagcacgtg gtatttccca ccgaaggccc ggctgcgttc
      961 cagggccgcc agatcgccca cgtcctccac cacgcaaagc aggcggtcgt cccggtgggg
     1021 gtcccggcag cggtcgcagg gaccgtcggc ttcgggcagg accgcggcgt cggccgcttc
     1081 cagcggaggg ccttccacaa agtcgccgca aagagggcac cggcgcatgc gggcccgggc
     1141 gtccgccagg gccaggagca gggcgtcccc cgcgtcccgg cggcggatca aatgaagggc
     1201 cgcgcgttcc gccattttgg gtcccacgcc gggcatcccc aggagggcgg acaccaatcg
     1261 gtcccaggcg ccggtcccgt tcatgccccg ggttttcctt ccgcgcgggg tttggaagcg
     1321 gcggcggggg cggattccgc ggggtcggcc ttccggattt tgccggggaa tttatccaaa
     1381 acctttttga gccccgcgtc cagggcgccc agggcccggg ggtcctgttc ttcgccttcg
     1441 gtggcggggc tttcgaccgg gggcggggcg tcggcttcct cgtccacgac ttcctcggct
     1501 tcctcgacca ccggcgccgg ttggggacgg ggagcggccg gcgcgggggg cggtgcggcg
     1561 accgtcaagg tgacggtcac gggacggccc gcgtgggcgc gcaggagatc cgaccagaag
     1621 acctgctggt tgaccaattg ttgtttctga aattcctggg cgcaggccag ctccagcccg
     1681 ccgccggaat cgaaccgcgg ccgggcgctg gagagaaaag gggccagaga cggtttttta
     1741 atttcaacgt cggcgagggt cttcacccac gcagcgcgaa gcccgtcggg ccccgtcaag
     1801 ggaacgaccg gggcgggcgt cggcgtttcg gccacgcgca gg
//
LOCUS       DAOQLS010000454         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45230_length_1842_cov_4.536654, whole genome shotgun sequence.
ACCESSION   DAOQLS010000454 DAOQLS010000000
VERSION     DAOQLS010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45230_length_1842_cov_4.536654"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK362_08370"
     CDS             complement(<1..255)
                     /locus_tag="PK362_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNC74877.1"
                     /translation="MNELTPPQRKIFDFISQQVEEVGVPPTVREIARRFDVAIGTVQG
                     HLKALDRKGVVRRMRDRARGLALAVKRDWTGSLRLPVLGRV"
     gene            326..1261
                     /locus_tag="PK362_08375"
     CDS             326..1261
                     /locus_tag="PK362_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74878.1"
                     /translation="MRPAALLLAALGLAPPVLAADAPYGDAFWKHWGDGQAEVAGYDL
                     VFPRYGHPRRGVAVAVFVTEPFSESLQVKADPGRHPAADEFQVMKLNLMRDFSTGLYD
                     YNLMTSAFVGLTPAGGRPAGTLAKVAFSAQEWCGQVFHQLNFRDGYVDESWRSYFDGE
                     GDGEHRHKIPPEGLSEDVLPLWARGLAAPFLNPGERKTRPILRSLTTARLLHKPLEWG
                     TGALTRSASPQKVTVPAGTFTVDVLTADLAEGRKWTFFVERTDARRLIKWTASDGESG
                     ELLGADRMAYWQMNGPGFEAQLKKIGLSPRPRRTP"
     gene            1258..1617
                     /locus_tag="PK362_08380"
     CDS             1258..1617
                     /locus_tag="PK362_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74879.1"
                     /translation="MNRGLPLGLVVCLSAAAGCRAPTPAEPPRASVAELDLRLKARAQ
                     QAERLRARADNTAHRNRRDINRALDDLEEFRTVAAMKLETLRAADAAHRSQAQDEAEA
                     ALADLDASLKITEEKIQ"
     gene            1614..>1842
                     /locus_tag="PK362_08385"
     CDS             1614..>1842
                     /locus_tag="PK362_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74880.1"
                     /translation="MKLPRFRTVLLVLFLAPVALLAAAALFIKLKYPPEKLRALALKW
                     AGDNLHRPVDLGEIHLGLSGLDVSKLSIRERT"
BASE COUNT          337 a          629 c          553 g          323 t
ORIGIN      
        1 cacccgcccc aacaccggca ggcggagact ccccgtccaa tcccttttca ccgccagagc
       61 caggccccgg gcccggtcgc gcatccggcg aacgaccccc ttccggtcca aggccttcaa
      121 atgaccctga acggtaccga tggccacgtc aaaacgccgg gcgatttccc gaaccgtggg
      181 cggcacgccg acttcctcca cttgctggga gataaaatcg aatattttgc gttggggcgg
      241 ggtcaattcg ttcatggccg aaataccata catttgtatg gtccccctgt caaggcggga
      301 atttcaaaat gataaaatcc cgtcgatgag acccgccgcc ctcctgctgg ccgccctggg
      361 cctggctccg cccgttctcg ccgccgacgc cccctacggc gacgcctttt ggaaacactg
      421 gggcgacggc caagccgagg tggcggggta cgacttggtt ttcccccggt atggacaccc
      481 gcgccggggc gtggccgtgg cggtcttcgt cacggaacct ttttccgaat ccctgcaagt
      541 caaagccgac ccgggccggc atcccgccgc cgatgaattc caggtgatga agctgaattt
      601 gatgcgggat ttttccaccg gcctctacga ttataatttg atgaccagcg ccttcgtcgg
      661 cctgacgccc gccggcggac gccccgccgg gaccctggcc aaggtggcct tttccgccca
      721 ggaatggtgc ggccaggtgt tccaccagct gaatttccgg gacggctacg tggacgaaag
      781 ctggcgcagc tacttcgacg gggaaggcga cggcgaacac cgccataaaa tccccccgga
      841 gggtctgagc gaagacgtcc tgcccctctg ggcccggggg ctcgcggcgc cctttcttaa
      901 tcccggggaa cgcaaaaccc gtccgatcct ccgctccctg accacggccc ggcttttgca
      961 caaacccctg gaatggggga ccggcgcctt gacccggtcg gcctcgcccc aaaaggtcac
     1021 ggtgcccgcc ggaactttta ccgtcgacgt tctcaccgcc gatctcgcgg aagggcgaaa
     1081 atggactttt ttcgtggaac ggacggacgc tcgacgactt attaaatgga cggcgtccga
     1141 cggcgaaagc ggagagttgt tgggcgcgga ccggatggcc tattggcaga tgaacggacc
     1201 gggctttgag gcccagttga aaaaaatcgg cctgtccccc cgtccccggc ggaccccgtg
     1261 aatcgcggac ttccccttgg gctggttgtt tgtctttcgg cggcggccgg atgccgggcg
     1321 ccgacacccg cggaaccccc gcgcgccagc gtggcggaat tggatttgcg attgaaagcc
     1381 cgggcccagc aagccgaacg ccttcgggcg cgagccgaca acaccgccca ccgcaaccgc
     1441 cgggacatca accgggccct ggacgacctg gaggagttcc gcacggtggc ggccatgaaa
     1501 ttggagaccc tgcgggccgc cgacgcggcc caccgttccc aggcccagga cgaggcggaa
     1561 gcggccctgg ccgacctgga cgcctccctt aaaattacgg aggaaaagat ccaatgaagc
     1621 tcccccgatt tcgcaccgtt cttctggttc tgttcctcgc cccggtggcc ctgctggcgg
     1681 ccgcggccct tttcattaaa ttaaagtacc cgcccgaaaa attgcgcgcc ctggccctga
     1741 aatgggccgg ggacaacctc caccgtcccg tcgatctcgg cgaaatccat ctgggcctct
     1801 ccgggctgga cgtgtcgaaa ctgtccattc gagaacggac gg
//
LOCUS       DAOQLS010000455         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45359_length_1839_cov_4.750561, whole genome shotgun sequence.
ACCESSION   DAOQLS010000455 DAOQLS010000000
VERSION     DAOQLS010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45359_length_1839_cov_4.750561"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1279
                     /locus_tag="PK362_08390"
     CDS             2..1279
                     /locus_tag="PK362_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNC74881.1"
                     /translation="MGDRSLAREVGRLTRVTAGLLEGVGPDVSLGEVCRVWDKAGKRF
                     YWAEAVGFRDDRIVMAPLGHVEGLGPGSFWMDGIGPVSVPVGDALLGRVVDARGAPLD
                     GGRPLALKERRPLRTAPPDPMSRRRIAEPLATGVRAIDGLMTCGKGQRLGLFAGSGVG
                     KSSLLGMIARGTQADVNVISLVGERGREVREFIEKDLGPEGLARSVVVVATSDQPPMA
                     RLHAALGATAVAEYFAEQGRDVLLMMDSVTRLAMAGREIGLALGEPPTTKGYTPSVFS
                     FLPRLLERAGNFSRGSVTGIYTVLVEADDMDDPVADTLRSILDGHVVLSRRMATQNHY
                     PAVDVLESLSRLMVDVTEAPHRRAAGILREVLAAHREAEDLLRVGAYARGSDAKIDFA
                     LAHLEKVKAFLKQDMNEKSDFPTTRAKLGALFA"
     gene            1301..1744
                     /locus_tag="PK362_08395"
     CDS             1301..1744
                     /locus_tag="PK362_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HNC74882.1"
                     /translation="MKKFRYRLETLLKLRFREEEKQAQALSQCQRRVAQVRQELERLD
                     GEWREKSRRTSAALVGALDVPRTENRMENLGRLLTAVSLRRTDLRLLETELTARLRRY
                     HDAVRDRKILERLRDIKLSEHQALRRRTETSFFDFVGATAVRRAV"
BASE COUNT          306 a          551 c          637 g          345 t
ORIGIN      
        1 cctgggcgac cgttccttgg cccgggaggt cggtcgcctc acccgggtga ccgccggtct
       61 gttggagggc gtcgggcccg acgtgagttt gggggaggtg tgccgggtct gggacaaggc
      121 gggaaaacgg ttttattggg ccgaggccgt ggggttccgg gacgaccgga tcgtgatggc
      181 gcccctgggt cacgtggagg gtctcggccc cggaagtttt tggatggacg gcatcggccc
      241 cgtgtcggtg ccggtgggcg acgccctctt gggccgcgtg gtggacgccc gtggcgctcc
      301 cctggacggc ggccgtccgc tcgccctcaa ggaacgccgc ccgctgcgga ccgccccgcc
      361 ggatcccatg agccgtcggc gaatcgcgga acctctcgcc acgggggttc gcgccatcga
      421 tggattgatg acctgcggga agggccagcg gttggggttg ttcgccggca gcggcgtggg
      481 aaaaagctcg cttctgggaa tgatcgcccg gggaacccag gccgatgtca acgtcatttc
      541 gctcgtgggg gagcgggggc gggaggttcg ggaatttatt gaaaaagacc tcgggcccga
      601 gggattggcg cgttccgtgg tcgtcgtggc gacctcggac cagccgccca tggcccgttt
      661 gcacgcggcc ctgggggcca cggccgtggc ggaatatttc gccgaacaag gccgggacgt
      721 tcttttgatg atggattccg tcacccggct ggccatggcc ggccgggaaa tcggcctggc
      781 cctgggggaa ccgcccacga ccaaagggta caccccctcg gtgttctcct ttttgccgag
      841 gctcctggag cgcgctggga atttttcccg agggagcgtt acggggatct acaccgtcct
      901 ggtggaagcc gacgacatgg acgatcccgt ggcggacacg ctgcggtcca ttttggatgg
      961 gcacgtcgtc ttgtcccggc gcatggccac ccaaaaccat tacccggccg tggacgtcct
     1021 ggaaagtttg agccgcctga tggtcgacgt gacggaggct ccccatcggc gggcggcggg
     1081 aatcctccgg gaagtgctgg cggcccatcg ggaggcggag gacttgctcc gggtgggggc
     1141 ctacgcccgg gggagcgacg ccaaaatcga cttcgccttg gcccatttgg agaaggtaaa
     1201 agccttttta aagcaggaca tgaacgagaa aagcgatttt ccgaccaccc gggcgaagct
     1261 tggcgcgctt tttgcataga gaagggtggg aggcgacacc atgaagaaat tccgttatcg
     1321 gctggaaacc cttctgaaac tgcgctttcg ggaagaagaa aagcaggccc aggccttgtc
     1381 ccaatgtcaa cgccgggtcg cacaggtccg gcaggaactc gaacgcctgg acggcgaatg
     1441 gcgcgagaaa tcccgacgga cgtcggcggc cctggtcggc gcgttggatg ttccacggac
     1501 cgagaaccgc atggaaaacc tcggccgttt gctgaccgcg gtctcgcttc gccgtacgga
     1561 ccttcggttg ctggaaaccg aattgaccgc tcgccttcgc cgatatcacg acgccgtccg
     1621 ggaccgaaaa atccttgaac gtttgcggga cattaaattg tccgagcatc aagcccttcg
     1681 gcggcggacc gaaacgtcgt tttttgattt tgtcggggcc acggcggttc gccgggccgt
     1741 ttgacgtgtc cttcgcccga tccctccttt ggcccgccgt cgtgacgtcc ctgtccacga
     1801 cctatttcct gaccgtcgga atccaggaaa aggtcctcc
//
LOCUS       DAOQLS010000456         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45581_length_1835_cov_3.211236, whole genome shotgun sequence.
ACCESSION   DAOQLS010000456 DAOQLS010000000
VERSION     DAOQLS010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45581_length_1835_cov_3.211236"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK362_08400"
     CDS             <1..969
                     /locus_tag="PK362_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74883.1"
                     /translation="PVGFGSPQGEIIPLRDESGNLSGYKKDKNGQTVLSKMDEKALRD
                     MAEKTGGTYFPASRGEIEVPRLLTAFRQMEKRSLESRVYGQLENRYRWPLWLAVGLLL
                     LEFLVPESGGWGRRLWKGLRSSVPLAAALVFLTPAARAMGRAPTAGELSRAVEKNPED
                     PRAQFQWGQGLYREGSYAAAAEAFEKVSSRASAPELRSAALYNAGNAYFRQGKTDEAI
                     KKYKEALRLFPSDPDAKHNLEFARKLKKAGGKPRPQPGSPKDDKKPRDGSGSSGEPPI
                     NESKGAGTMTKEDAERLLQAVEDQEKRAQRRARRGQPPEPPQGPDW"
     gene            982..>1835
                     /locus_tag="PK362_08405"
     CDS             982..>1835
                     /locus_tag="PK362_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNC74884.1"
                     /translation="MKRLWTVLLFLTAGPLRAGDLSFEATVDRDTLTMNDSLTLQLSI
                     SGAGLSVSRPELPSLPGFRAVFAGQSQNVSFVNGTVSNQVVYTYALEPQAPGEYTLPS
                     LTMEAGGQKASSSPIKIKVLSAGAAPAPRTGGGPAPSDGGRDLFVTTTLDKKNPVVGE
                     TVVLSFRFYSRAPLLSQPRYQAPDVTGFLTEDLPPQRQYTEIIGGRRYQVVELRTALY
                     ATAPGRLTVGPAALECSVQDFSRESFPGGFPSFFDEAFGAAKRVVLRSDPISVNVARA
                     RSAPPATSA"
BASE COUNT          349 a          600 c          575 g          311 t
ORIGIN      
        1 cccgtcgggt tcggcagtcc ccagggcgaa atcattccgt tgcgcgacga atccggcaac
       61 ctgtccggct ataaaaagga taaaaacggc cagaccgtcc tgtcgaagat ggacgaaaaa
      121 gcccttcggg acatggccga gaagaccggc gggacttatt tcccggccag ccgcggcgaa
      181 atagaggtgc cgcgcctttt aacggcgttc cgccaaatgg agaagcggtc cctggaaagc
      241 cgcgtctacg gccaattgga aaatcgctac cgatggcccc tgtggctcgc ggtcggcctg
      301 ttgctcctgg aattccttgt tccggaatcg ggggggtggg gacggcgcct ttggaagggc
      361 cttcgctcgt cggtgcctct ggcggcggcc ctggtgtttt tgacccccgc ggcccgggcc
      421 atggggcgag ccccgaccgc gggggagctt tcccgggcgg tggaaaaaaa tcccgaggac
      481 ccccgggctc aatttcaatg ggggcagggc ttgtaccggg agggaagtta cgcggcggcg
      541 gccgaggctt ttgaaaaggt ttcttcccgg gcgtcggcgc cggagcttcg aagcgccgcc
      601 ctttacaacg ccggcaacgc ttattttcgc caaggaaaaa cggacgaagc gatcaaaaaa
      661 tacaaggagg cccttcgtct ctttccgtcg gatccggacg ccaagcacaa tttggaattc
      721 gcccggaaat tgaaaaaggc cgggggaaaa ccccggcccc aacccgggtc cccgaaggac
      781 gataaaaagc cccgggacgg gagcgggtcc tcgggcgaac ccccgataaa cgaatcgaag
      841 ggggccggga ccatgaccaa ggaggacgcg gaacgattgt tgcaggccgt cgaggatcag
      901 gaaaaaagag cccagcggcg cgcccgtcgc ggccaacccc cggaaccgcc ccagggcccg
      961 gactggtaaa ataaaccgct catgaaaaga ctttggacgg ttcttctttt tttaacggcc
     1021 ggacccctcc gcgccgggga tctttccttc gaggccaccg tggaccggga caccctgacc
     1081 atgaacgaca gcctgaccct tcagctctcg atttccgggg cgggcctctc ggtgtcccgg
     1141 ccggaactgc cgtccctccc gggattccgg gccgtgttcg cgggccagtc ccagaacgtg
     1201 agcttcgtga acgggacggt ttccaaccag gtggtttaca cctacgcctt ggagccccag
     1261 gcccccgggg aatacactct gccgtctctc accatggaag ccggcggcca gaaggcctcc
     1321 agttcgccga ttaaaatcaa agtcctttcg gccggcgccg ccccggcgcc ccggaccggc
     1381 ggcggccccg caccgtccga cggcggccgg gacctctttg tgacgacgac cctcgataaa
     1441 aagaatcccg tggtgggcga aacggtggtc ctgtcctttc ggttttacag ccgggccccc
     1501 ctcctttccc agccccggta ccaggccccg gacgtcacgg gatttttgac cgaggacctg
     1561 ccgccccagc gccaatacac cgaaatcatc ggcgggcgac gctatcaagt ggtggaactg
     1621 cgaacggccc tttacgccac ggccccgggc cggttgaccg tcggacccgc ggccctggag
     1681 tgcagcgttc aggatttttc ccgtgaatcc tttccggggg ggttcccgtc cttctttgac
     1741 gaagccttcg gcgccgccaa acgcgtggtc cttcgaagcg atccgatctc cgtgaacgtc
     1801 gcgcgcgcac gcagcgcgcc tccggcgacg agcgc
//
LOCUS       DAOQLS010000457         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45616_length_1834_cov_4.736931, whole genome shotgun sequence.
ACCESSION   DAOQLS010000457 DAOQLS010000000
VERSION     DAOQLS010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45616_length_1834_cov_4.736931"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PK362_08410"
     CDS             <1..1162
                     /locus_tag="PK362_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNC74885.1"
                     /translation="QLLTETVRDHLVSDVPLGLFLSGGLDSATIAHYARAAGKTLDSY
                     TIYFGDASFSERREAAETAKRLDLRHHEEEMRPSTEVLARLGEILDEPLADPSVLPTY
                     YLCRLARRGVTVTLSGDGGDELFAGYPTYLADRYAAWFRRLPAAAQRWAVQLANAAPT
                     SFKRIPWDYRLKAFVGAAARPQPDAHFGWLEAFQREEKERLFTPDFWASVQRHPPEQS
                     FREAFEDGRHRPFLERLLYLDQRTRLLDQYLVKVDRLSMANSLEVRVPLLDNALVEFA
                     STVPANHKIRGWTTKYLFRRLMKDRLPASVLTGKKKGFSPPLAQWLAGPLKTWAEDLL
                     SPESLSRTGILRPEFPRALLAEHVARRRDNHRRLWVLLSFVQWHRAYAAPVA"
     gene            1227..>1834
                     /locus_tag="PK362_08415"
     CDS             1227..>1834
                     /locus_tag="PK362_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74886.1"
                     /translation="MEKNHRRVSALLAGALLGFAVSLRAKTSDWDSPTLWSVDTDDQS
                     SLSVRPVEGIDGLRLELKYTLLGAGHRWVQMKREWDPAALFDGRPLTFLFRANGTATM
                     EIKLVDQDGSNFICRYPLKQYGAKWEPVVLYRDSFEYGWGGNATLDRPKQLAIAISGT
                     AENGVVYIDEIGLGRKGQPATQTPDGPRLDPRRFKTGFGFAAR"
BASE COUNT          310 a          648 c          552 g          324 t
ORIGIN      
        1 ccagctgctg accgagaccg tgcgggacca tttggtgtcg gacgttcccc tggggctgtt
       61 cctctccggc gggctggatt cggccaccat cgcccactac gcccgggccg cggggaaaac
      121 gctcgacagc tacaccattt atttcgggga cgcgtccttc tccgagcggc gcgaggcggc
      181 ggaaaccgcg aaacggctgg acctccgcca ccacgaggag gagatgcgcc cctcgaccga
      241 ggtgctggcc cgcctggggg aaatcctgga cgaacccctg gccgacccgt cggtcctgcc
      301 cacctactat ttgtgccggc tggcccgccg gggcgtcacc gtcaccctct cgggggacgg
      361 cggggacgaa ttgttcgccg gttaccccac ctatttggcg gaccgttacg cggcgtggtt
      421 ccggcgtttg ccggccgcgg ctcagcggtg ggcggtccaa ctggccaacg cggcgccgac
      481 ttcctttaaa cggattccct gggattaccg gttgaaagcc tttgtgggcg cggccgcccg
      541 gccccagccc gacgcccatt tcggctggtt ggaagcgttc caacgggagg aaaaggagcg
      601 gcttttcacg ccggattttt gggcttccgt ccagcgccac cccccggaac agagttttcg
      661 ggaggccttt gaagacggcc gccaccggcc gttcctggaa cggctgctct atctggacca
      721 gcggacgcgg ctcttggacc aatacctcgt gaaagtcgat cggttgagca tggcgaattc
      781 cctggaagtc cgggtgcccc tgctggacaa cgcgctggtg gagttcgcct ccacggtgcc
      841 cgccaaccac aagatccggg ggtggaccac caaatatctt ttccgacggc tgatgaaaga
      901 ccgcctgccc gcctccgtcc tgaccggcaa gaagaaagga ttttccccgc ccctggccca
      961 gtggctcgcc gggcccctca aaacctgggc cgaggatctg ctctcgccgg agtccctgtc
     1021 ccgcacggga atcctgcgtc ccgaatttcc ccgggccctg ctggcggaac acgtcgcccg
     1081 tcggcgggac aaccaccgtc ggctgtgggt cctcctgagt ttcgtgcagt ggcaccgggc
     1141 ctacgccgcc cccgtcgcgt aaaagtttac acaccgttta caagaattta ggacgacctt
     1201 tttcccccgg gggttaaact ttccccatgg aaaaaaacca ccgccgcgtt tcggcgcttc
     1261 tcgcgggagc gctgttgggt ttcgccgttt cccttcgggc caaaacctcc gactgggaca
     1321 gccccacgct gtggtccgtc gacaccgacg accagagctc gctctccgtc cgcccggtgg
     1381 agggcatcga cggcctccgc ctggaattga aatacacgct cctgggcgcc ggacaccggt
     1441 gggttcaaat gaaacgggaa tgggaccccg ccgccctttt cgacggccgg cccctcactt
     1501 tcctattccg cgccaacggc accgccacca tggaaatcaa gctggtcgac caggacggct
     1561 ccaatttcat ctgccggtac cccctcaaac agtacggcgc caaatgggaa cccgtcgtcc
     1621 tttaccggga cagctttgaa tacggctggg gcggcaacgc caccctggac cgccccaaac
     1681 aactggccat cgccatctcc gggacggcgg aaaacggcgt ggtctacatc gacgagatcg
     1741 gcctcggccg caagggccag ccggccaccc agacgcccga cggcccgcgc ctcgaccccc
     1801 gccgctttaa aacgggcttc ggtttcgccg cccg
//
LOCUS       DAOQLS010000458         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45841_length_1829_cov_4.012965, whole genome shotgun sequence.
ACCESSION   DAOQLS010000458 DAOQLS010000000
VERSION     DAOQLS010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45841_length_1829_cov_4.012965"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /gene="atpC"
                     /locus_tag="PK362_08420"
     CDS             complement(<1..330)
                     /gene="atpC"
                     /locus_tag="PK362_08420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNC74887.1"
                     /translation="MSARIPVSIVTPERPTWEGEVDSLVLPAFEGQLGILPGHAPLLA
                     ELKPGTVVMRDGPETKLLAVSGGFVEVHGGRVSVFAETAEIADEIDAERARQAAERAR
                     AKIKSTPA"
     gene            complement(334..1071)
                     /locus_tag="PK362_08425"
     CDS             complement(334..1071)
                     /locus_tag="PK362_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74888.1"
                     /translation="MERTIGQRLTPASLFQWAGRMARHPWQFFREVATEGGYATPIYY
                     ALFWQLIAGALGFAVGFVPGATSLSVGGRLMWLVFGPPLTLGVGFFLSAIFFVIWHLM
                     GSPHPFRTAFRCWALLTPLSALSAASRVVPFLFLAVMIYGFFLLIVATVEVHGISLKK
                     ALVVWILVLVVSLALQVMAMIFRAVRTRLFNGGEMLPGAYRQGDPPRLPGQGMLPADL
                     QKQVEAEMAKHRAEVEKQTKESVPAKR"
     gene            complement(1094..>1829)
                     /gene="atpD"
                     /locus_tag="PK362_08430"
     CDS             complement(1094..>1829)
                     /gene="atpD"
                     /locus_tag="PK362_08430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNC74889.1"
                     /translation="VALTALTQAEYFRDKAGQDVLLFIDNIFRFTQAGSEVSALLGRM
                     PSAVGYQPTLSTEMGELQERITSTKKGSVTSVQAIYVPADDLTDPAPATAFAHLDATT
                     VLSRQIAELGIYPAVDPLDSTSRILDPKIVGQEHYDTARAVQRVLQRYRDLQDIIAIL
                     GIDELSEEDKLLVARARKIQKFLSQPFHVAEQFTGIPGRYVALKDTIRGFRELVDGKW
                     DHLPEQAFFMVGGIEEAAEKAKGLTQ"
BASE COUNT          355 a          581 c          582 g          311 t
ORIGIN      
        1 ggccggggtg gatttgattt tggcccgggc ccgttcggcg gcctgacggg ctcgctcggc
       61 gtcaatctcg tccgctattt cggcggtctc ggcgaaaacg gaaacccgtc cgccgtgaac
      121 ctccacgaag ccgcccgaca ccgccaaaag ttttgtttcc ggcccgtccc gcatgaccac
      181 cgtgcccggc ttcaattcgg ccaacagagg cgcgtggccg ggcaaaatgc ccaactgccc
      241 ctcgaacgcg ggcaacacca gggagtccac ttccccctcc caggtgggcc gttcgggtgt
      301 cacgatgctg acgggaatgc gggcgctcat ggctcagcgt ttggctggaa cgctctcctt
      361 cgtttgtttt tcgacttcgg cgcggtgttt ggccatttcg gcttccacct gcttctgcag
      421 gtccgcgggg agcattccct ggcccggtag ccggggcggg tcgccttggc ggtacgcgcc
      481 ggggagcatt tcaccgccgt tgaaaagccg ggttcgaacg gcgcggaaaa tcatggccat
      541 cacctggagc gccaaagaca ccaccaacac caaaatccac acgacgagcg ccttcttgag
      601 ggagatcccg tggacttcca ccgtggccac gatcaacagg aaaaacccgt aaatcatgac
      661 ggccaggaac aggaacggga ccacgcgcga ggcggcgctc agggcgctga ggggcgtgag
      721 caacgcccag caccggaacg ccgttcggaa gggatgcgga gaccccatca ggtgccaaat
      781 cacgaagaaa atcgccgaca ggaaaaagcc gacgccgaga gtcaggggcg gtccgaaaac
      841 caaccacatc aaccggccgc ccacgctcaa ggaggtcgcc ccgggaacga accccacggc
      901 aaaccccagg gcccccgcga tcaactgcca aaagagcgcg tagtagatgg gcgtcgcgta
      961 cccgccttcc gtcgccactt cgcgaaagaa ctgccagggg tgacgcgcca tccgtccggc
     1021 ccattgaaaa agggacgcgg gggtcaagcg ttgaccgatc gttcgttcca tggatcctcc
     1081 tcgagggccc gggttactgg gtcaggcctt tggccttttc cgccgcttcc tcaatgccgc
     1141 cgaccataaa aaacgcctgc tcgggcaggt gatcccactt gccgtccacc agttcgcgga
     1201 acccgcggat ggtgtccttg agcgcgacgt atcggccggg aatgccggtg aactgctccg
     1261 ccacgtggaa gggctgggac aggaattttt ggatcttccg ggcccgggcc accagcagct
     1321 tgtcctcttc ggacagttcg tcgatgccca aaatggcgat gatgtcctgc aggtcccggt
     1381 agcgctgcaa cacgcgctgc accgcccggg ccgtgtcgta gtgttcctga ccgacgatct
     1441 tgggatccag aatgcggctg gtcgaatcca gcgggtccac ggcggggtaa atacccaact
     1501 ccgcgatctg acgggaaagc acggtcgtgg cgtccaagtg ggcgaaggcc gtggccggcg
     1561 cggggtcggt caagtcgtcg gccggaacgt aaatggcctg gacggaggtg acggagcctt
     1621 ttttggtcga ggtgatgcgc tcctgcaatt cgcccatttc cgtggagagc gtgggctggt
     1681 accccacggc cgagggcatg cggcccagca gggccgacac ttcggacccg gcctgggtga
     1741 agcggaaaat attgtcgatg aacaacaaca cgtcctggcc cgctttgtcg cggaaatatt
     1801 cggcctgggt taaggccgtc aaggcgacc
//
LOCUS       DAOQLS010000459         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_45969_length_1826_cov_4.761717, whole genome shotgun sequence.
ACCESSION   DAOQLS010000459 DAOQLS010000000
VERSION     DAOQLS010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45969_length_1826_cov_4.761717"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK362_08435"
     CDS             <1..235
                     /locus_tag="PK362_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNC74890.1"
                     /translation="VSVMIDTHGTGAVPEERLVEMARDVFPLTPRGIIDYLKLRRPFY
                     RKTASYGHFGRNDADFYWEKTNKAAELKKLARV"
     gene            257..1513
                     /gene="ahcY"
                     /locus_tag="PK362_08440"
     CDS             257..1513
                     /gene="ahcY"
                     /locus_tag="PK362_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNC74891.1"
                     /translation="MKYHVKDLRLAADGENRITWAEREMPVLRLIKARFKKERPLKGV
                     RLSACLHVTTETANLAITLKEGGADVVLCASNPLSTQDDVAAALVKKHGIRTYAIKGE
                     DNKTYYKHINAALDHRPQITMDDGADVVGVLHKERPEQLPEVIGGTEETTTGVIRLRA
                     MEKDGVLEYPIVAVNDALTKHMFDNRYGTGQSTLDGILRATNVLFAGKNIVVSGYGWC
                     GRGVATRAHGLGGHVIVTEVDPFRALEAVMDGFAVMPMDKAAALGDVFITVTGDIGVL
                     RAEHFKKMKDGAILANSGHFNVEIDIPALAKMAKTKREARPFVDEYTLPGGRRIHVLG
                     EGRLINLASAEGHPASVMDMSFANQALCAEYMVKNAKGLAKKVHSVPVEIDREIARLK
                     LAGMGVRIDRLTPEQEKYLASWQEGT"
     gene            1598..>1826
                     /locus_tag="PK362_08445"
     CDS             1598..>1826
                     /locus_tag="PK362_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74892.1"
                     /translation="MKPGRWSIAVPLALLVAGAGCKNDNLFTGLHRQGTGDTASLVSD
                     GVAALSRGDYAAAERYFEDALVQDPSNSDALY"
BASE COUNT          393 a          626 c          529 g          278 t
ORIGIN      
        1 cgtgagcgtc atgatcgaca cccacggcac cggggcggtg cccgaggaac gcctggtgga
       61 gatggcgcgg gacgttttcc cgctcacgcc ccggggcatc atcgattatt taaaactgcg
      121 gcgtcccttc taccgcaaaa ccgcctccta cggtcatttc ggccggaacg acgcggactt
      181 ctactgggaa aaaaccaaca aagcggccga gctcaaaaag ctcgcccgcg tctaacccct
      241 taaaggaccc ccccccatga aataccacgt caaggacctt cgtctcgccg ccgacggcga
      301 aaaccggatc acctgggccg aacgggaaat gcccgttctg cggctcatca aggcgcgctt
      361 caagaaggaa cggccgctga aaggcgtccg gctttcggcc tgcctccacg tcacgaccga
      421 aaccgccaac ctggcgatca ccttaaagga aggcggcgcg gacgtcgttc tgtgcgcttc
      481 caacccgctc tccacccagg acgacgtggc ggcggctctg gtcaaaaagc acggcatccg
      541 gacctacgcc atcaaaggcg aggacaacaa gacctattac aagcacatca acgccgccct
      601 ggaccaccgc ccccaaatca ccatggacga cggggccgac gtggtcggcg tgcttcacaa
      661 ggaacgcccc gagcagctgc ccgaggtcat cggcggcacc gaagagacca ccaccggcgt
      721 catccgcctt cgggccatgg aaaaagacgg cgtgctggaa taccccatcg tcgcggtcaa
      781 cgacgccctg accaagcaca tgttcgacaa ccgttacggc accggccaat cgaccttgga
      841 cggcatcctc cgggccacca acgttctctt cgccggtaaa aacatcgtgg tctccggcta
      901 cggttggtgc ggccgcgggg tggccacccg ggcccacgga ctgggcggcc acgtgatcgt
      961 gaccgaagtg gaccccttcc gcgcgctcga agccgtcatg gacggcttcg ccgtgatgcc
     1021 catggacaag gccgccgccc tgggcgacgt gttcatcacc gtcaccggcg acatcggcgt
     1081 tctgcgggcc gaacatttca agaaaatgaa ggacggcgcc attctcgcca actccggcca
     1141 cttcaacgtg gaaatcgaca tcccggccct ggccaaaatg gccaaaacga aacgggaggc
     1201 ccgccccttc gtggacgaat acaccctgcc cggcggccga cggatccacg tcctgggcga
     1261 aggccgcctg atcaacctgg cctccgccga aggccacccg gcgagcgtca tggacatgtc
     1321 cttcgccaat caggccctct gcgccgaata catggtgaaa aacgccaaag ggctggccaa
     1381 gaaggtccac agcgtcccgg tggaaatcga ccgcgaaatc gcccgcctga agctggccgg
     1441 catgggcgtg cgcatcgacc gactgacgcc ggaacaggaa aaatacctgg cttcctggca
     1501 ggaaggcact tgatttaacc ggttgtctcc gatatcctag acggacatcg gccgggcggg
     1561 ccaaccgcgc cggaatatcc ccgggaggaa acagtctatg aaaccaggac gatggtccat
     1621 cgccgtaccc ttggccctgt tggtcgcggg cgcgggttgc aaaaacgaca atctcttcac
     1681 ggggcttcac cgccaaggaa ccggggacac cgccagtttg gtgtccgacg gcgtggcggc
     1741 tctctcccgg ggggactacg cggcggccga acgttatttt gaggacgccc tggttcaaga
     1801 cccctccaac tccgacgccc tctacg
//
LOCUS       DAOQLS010000460         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46209_length_1820_cov_5.715581, whole genome shotgun sequence.
ACCESSION   DAOQLS010000460 DAOQLS010000000
VERSION     DAOQLS010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46209_length_1820_cov_5.715581"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PK362_08450"
     CDS             complement(<1..545)
                     /locus_tag="PK362_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNC74893.1"
                     /translation="MAQNAMQQKIIENLWDSSRTLRELANEAPAIEKAARAIVKSLRG
                     GHKLLAFGNGGSAADAQHLTAELSGRFEKDRRGLPAVALTTNPSAVTAIANDYSYDDI
                     FSRQMEGLVKRGDVVVAISTSGNSANVLAGAKEAKKVGATVIAWTGKGGGKLKALADV
                     CLDVPSQRTARIQEGHLALLHT"
     gene            complement(555..>1820)
                     /gene="accC"
                     /locus_tag="PK362_08455"
     CDS             complement(555..>1820)
                     /gene="accC"
                     /locus_tag="PK362_08455"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNC74894.1"
                     /translation="SSLHVRYADESVCIGPATAAESYLNIPAIISAAEITGADAIHPG
                     YGFLSENTHFAEVCESCNITFIGPSKDAIRRMGDKAEARKIMGDYGVPIIPGTKGCIG
                     PDDKELFKIAKKIGFPILVKAKAGGGGKGMRVVQDASFLRDAILAATHEAKTAFGNGD
                     VYLERYLKAPRHIEVQVLGDQHGNVVSFPERDCSVQRRHQKLIEESPSPMVSDSLRKK
                     MGKAARLAAKAVKYSTVGTVEFLYDEESDDFFFIEMNTRIQVEHPVTEMVTGIDLIKE
                     QIRAAAGEKLPFESEDVQIRHHAMECRINAEDPARNFMPSPGKITNLILPGGPGVRVD
                     THMYTGYTIPTYYDSLMAKLICATRYGAGGGREHTIRRMRRALDEFIVEGVKTTIPFH
                     RDVMAHEAFLKGDVQTDFIEKHLAVTTAA"
BASE COUNT          273 a          551 c          619 g          377 t
ORIGIN      
        1 gtgtggagaa gagccaggtg gccttcctgg atgcgcgccg tccgctggga ggggacgtcc
       61 aggcacacgt cggccaaggc tttgagtttc cccccgcctt tgcccgtcca ggcgatgacc
      121 gtggcgccga ccttcttggc ttccttggcc cccgccaaca cgttggcgga attgccggag
      181 gtggaaatgg ccacgaccac gtcgccccgc ttcaccaggc cttccatctg ccgggagaag
      241 atgtcgtcgt aggaatagtc gttggcgatg gcggtgacgg ccgaggggtt ggtggtcagg
      301 gcgacggcgg gcaggccccg gcggtctttt tcaaaacggc cggagagctc cgcggtcaga
      361 tgctgggcgt cggcggcgga gcccccgttg ccgaaggcca acaatttgtg cccgccgcgc
      421 agggatttga cgatggcccg ggcggccttc tcgatggccg gcgcctcgtt ggccaattcg
      481 cgcagggtgc ggctcgaatc ccagaggttc tcgatgatct tctgctgcat cgcgttttgg
      541 gccatgggcc ggacctaggc cgccgtggtg acggccaaat gcttttcgat gaaatccgtc
      601 tggacgtccc ctttgaggaa cgcttcgtgg gccatgacgt cccggtggaa cgggatcgtc
      661 gttttgacgc cctcgacgat gaattcgtcc agggcgcgcc gcatgcggcg gatggtgtgc
      721 tcccggccgc cccccgcgcc gtagcgcgtg gcgcagatga gcttcgccat caggctgtcg
      781 tagtaggtcg gaatggtgta gcccgtgtac atgtgggtgt ccacccggac gcccgggccg
      841 ccgggcaaaa taaggttcgt gattttcccg ggggagggca tgaaattccg ggccgggtcc
      901 tcggcgttga tgcggcattc catggcgtgg tgccggatct ggacgtcctc ggattcgaag
      961 ggcagtttct cgccggccgc ggcgcggatc tgctctttga tcaagtcgat gcccgtcacc
     1021 atctcggtga ccgggtgctc gacctggatc cgggtgttca tctcgatgaa gaaaaaatcg
     1081 tcggactcct cgtcgtaaag gaattccacg gtgcccaccg tcgaatactt cacggccttg
     1141 gcggccaggc gcgcggcctt gcccatcttc ttccgcaggc tgtcgctcac catgggggac
     1201 ggcgactcct cgatcaattt ctggtggcgg cgctgcaccg agcagtcgcg ctcggggaag
     1261 ctcaccacgt tgccgtgctg gtcgcccagc acctgcactt cgatgtggcg cggggctttc
     1321 aggtaccgtt ccaaatagac gtcgccgttg ccgaaggcgg tcttcgcttc gtgggtggcg
     1381 gccagaatgg cgtcccgcag gaagctcgcg tcctgaacca cgcgcatgcc tttgccgccg
     1441 ccccccgcct tggccttgac caggatcggg aaaccgattt tcttcgcgat cttgaaaagt
     1501 tccttgtcgt cggggccgat gcaccctttg gtgccgggaa tgatgggcac gccgtagtcg
     1561 cccatgattt tacgcgcttc ggccttgtcg cccatgcggc ggatggcgtc cttggagggc
     1621 ccgatgaagg tgatgttgca gctttcgcac acttcggcga aatgggtatt ttccgagagg
     1681 aagccgtacc cggggtggat ggcgtcggcg ccggtgattt cggccgcgga gatgatcgcc
     1741 ggaatgttca ggtagctttc ggccgccgtg gcgggcccga tgcagaccga ttcgtccgcg
     1801 taacggacgt gcagggaact
//
LOCUS       DAOQLS010000461         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46225_length_1820_cov_3.447025, whole genome shotgun sequence.
ACCESSION   DAOQLS010000461 DAOQLS010000000
VERSION     DAOQLS010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46225_length_1820_cov_3.447025"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK362_08460"
     CDS             complement(<1..277)
                     /locus_tag="PK362_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74895.1"
                     /translation="MVTRRLALADQEEAILLFGQHDKNLRELERRFAVQIFVRPSERA
                     NEAGLTLAVRGRPKPVDQALATLEEMRRTAVRSKQKGTRVESVEVPLP"
     gene            complement(446..>1820)
                     /gene="aspS"
                     /locus_tag="PK362_08465"
     CDS             complement(446..>1820)
                     /gene="aspS"
                     /locus_tag="PK362_08465"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006006894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNC74896.1"
                     /translation="LRHKVTQTVRDVLAAQGFLELETPFLTKSTPEGARDFLVPSRLN
                     AGTFYALPQSPQLFKQLFMVSGFDRYYQIARCFRDEDLRADRQPEFTQIDLEMSFIDV
                     DDVIALVETLLKAVFKAALGKDIPTPFPRLSYDEAMRRFGSDKPDLRFGMEIQDVSAV
                     FAETKFQVFANALKSGGVVRALCLPGGAAMSRTEIDKLTDWVKTFGARGLAWLKMTDK
                     GPESSIAKFMSEKELADLPKAVGAKTGDIVLFGAGAAADVSAYLGPLRVELARRAGWT
                     KTEGVFQFLWVVDFPLFEYSAEDKKWNAVHHPFTRPAPGDEKTLMAGGVPAEKLAAFR
                     SRAYDIVLNGTEIGGGSLRIHQTALQSKIFEILGISGESARAKFGFLLDALDFGAPPH
                     GGLALGLDRLVALLTGEDSIRDVIAFPKTQRGADPMSGAPSTVDEAQLLKELALRIAR
                     APEPKKA"
BASE COUNT          321 a          576 c          605 g          318 t
ORIGIN      
        1 cgggcaaagg gacctccacc gactccaccc gcgtcccctt ttgtttggac cggacggcgg
       61 tgcgccgcat ttcctccagc gtcgccaggg cctggtccac gggtttgggt cggccgcgga
      121 cggcgagggt caacccggcc tcgttggctc gttccgacgg acggacgaaa atctgaacgg
      181 cgaaccgccg ctccaattcg cgaaggtttt tgtcgtgctg gccgaagagg aggatggcct
      241 cctcctgatc cgccaacgcc aggcggcggg tcaccacggc gcgcgccccc cggatacaac
      301 agaaccccgt cggccgcccg cggggggcga cggatcgggg tccgtccaag aagtcgttcg
      361 cgtcagtccc aaaagggtgt cgagcatgcg caatgaaccg ccctcaagtg taacccccgg
      421 accgggcctt gtcaacggaa cggcctcagg cctttttcgg ttccggggcc cgggcgatcc
      481 gcagggcgag ttccttgagc aattgggctt cgtccacggt cgagggcgcg ccgctcatgg
      541 ggtccgcgcc ccgctgggtt ttggggaagg cgatgacgtc ccggatggaa tcttcgcccg
      601 tcaacagggc gaccaaccgg tccaatccca gggccaaacc gccgtggggc ggggcgccga
      661 aatccaaagc gtccagcaaa aagccgaatt tggcccgggc ggactcgccg gagatgccga
      721 ggatttcaaa gattttcgac tggagggccg tttgatgaat ccgcaggctg ccgcccccga
      781 tttccgtgcc gttcaagacg atgtcgtagg cccgggaacg gaacgccgcg agcttttccg
      841 ccgggacgcc cccggccatc agggtttttt cgtccccggg cgccggacgg gtgaaggggt
      901 ggtgcacggc gttccatttt ttgtcctcgg cgctgtattc gaagagcggg aaatccacga
      961 cccagaggaa ttgaaacacc ccctcggttt tggtccaccc cgcccggcgg gcaagctcca
     1021 cccgcagcgg accgaggtag gcggagacgt ccgccgcggc gccggcgccg aaaagcacga
     1081 tgtcgccggt cttggcgccc acggccttgg gcaaatcggc caattccttt tccgacatga
     1141 atttagcgat ggaggactcg gggcccttgt ccgtcatctt gagccaggcc aggccccggg
     1201 cgccgaaggt tttgacccag tcggtcagct tgtcgatttc cgtccgggac atggcggcgc
     1261 cgccgggaag gcagagggcc cggaccacgc caccgctttt cagcgcgttg gcgaacacct
     1321 ggaatttggt ttccgcgaac acggccgaca cgtcttggat ttccatgccg aagcgaaggt
     1381 ccggcttgtc ggacccgaag cggcgcatgg cctcgtcgta ggaaagccgg gggaagggcg
     1441 tcggaatgtc tttgcccagg gccgctttga acaccgcttt tagcagggtt tccaccagcg
     1501 cgatcacgtc gtccacgtcg atgaaggaca tttccaggtc gatctgggtg aattcgggct
     1561 ggcggtcggc gcgcaggtcc tcgtcccgga aacagcgggc gatttggtaa tagcggtcga
     1621 accccgacac catgaacaat tgcttgaaaa gctggggcga ctgggggagc gcgtagaacg
     1681 tccccgcgtt caaacgggag ggcaccagga agtcccgggc cccctcgggc gtggatttgg
     1741 tcaagaacgg cgtttccaat tccaaaaacc cctgggcggc caggacgtcc cgcaccgtct
     1801 gggtcacttt gtggcgaagg
//
LOCUS       DAOQLS010000462         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46316_length_1818_cov_4.920590, whole genome shotgun sequence.
ACCESSION   DAOQLS010000462 DAOQLS010000000
VERSION     DAOQLS010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46316_length_1818_cov_4.920590"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PK362_08470"
     CDS             complement(<1..292)
                     /locus_tag="PK362_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopropylmalate isomerase"
                     /protein_id="HNC74897.1"
                     /translation="MSLDSKIPSVQGRAVAVPGDDIDTDRIMPARYLRCVTFESIGQY
                     TFQDERFDAVGKPKAHPFNDPRHRGASILVVNKNFGCGSSREHAPQGILRWGI"
     gene            complement(289..1689)
                     /gene="leuC"
                     /locus_tag="PK362_08475"
     CDS             complement(289..1689)
                     /gene="leuC"
                     /locus_tag="PK362_08475"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNC74898.1"
                     /translation="MGKTLLDKVWDLHTVRELPNGQTQLFIGLHLVHEVTSPQAFQML
                     REKGLRVAYPGRTFATVDHIVPTDQQGRPFLDGLAEEMMVALERNCREFGVRFFDFAS
                     GEQGIVHVVGPEQGLTQPGLTVACGDSHTSTHGAFGAIAFGVGTTQIRDILATQTLAL
                     ARPKVRRVRVSGNLAAGVYAKDVILAIVRTLGVNGGIGFAYEFAGDTIERMSMEERLT
                     VCNMAIEGGARVGYINPDRTTVEFLKGRPYVPAGKDFDRAAAWWLSMGSDPDALFADN
                     VNLNAAELEPQIAWGINPGQTVGVTERLPRPEDSRNGSRAGLEEAYRFTSFEPGQEVR
                     QIPINVAFIGSCTNGRMSDLREAARVAKGRRVAGGVKAIVVPGSQKVQRQAEKEGLPE
                     VFRAAGFEWRNAGCSMCLAMNPDKLKGREICASSSNRNFIGRQGSPTGRTLLMSPAMV
                     AAAAIHGRVSDVREML"
BASE COUNT          312 a          612 c          555 g          339 t
ORIGIN      
        1 ggattcccca acggaggatt ccctgcggcg cgtgctcccg ggaggacccg cacccgaaat
       61 ttttgttcac caccaggatc gacgcccccc gatgccgggg atcgttgaac gggtgcgctt
      121 tgggttttcc gacggcgtcg aagcgttcgt cctgaaaggt gtattgcccg atgctttcaa
      181 acgtgacgca ccgcaggtag cgggcgggca tgatgcggtc ggtgtcgata tcgtcgcccg
      241 gaacggcgac ggcgcgccct tgaaccgacg ggattttgga atcgaggctc atagcatctc
      301 cctcacatcg gacacccgcc cgtggatcgc ggcggcggcc accatggccg gactcatcaa
      361 cagcgttcga cccgtgggac tcccctgtcg tccgatgaag ttgcggttgg aactggaggc
      421 gcagatttcg cgtcccttca gcttgtccgg gttcatggcc aggcacatgg aacacccggc
      481 gttgcgccac tcaaacccgg cggcgcggaa cacctccgga aggccctcct tttcggcttg
      541 ccgctgaacc ttttgagagc cgggcacgac gatggctttg acccctccgg ccacccggcg
      601 gcctttcgcc actcgcgcgg cttctcgaag gtcggacatt cgcccgttgg tgcaggagcc
      661 gatgaaagcc acgttgatgg ggatttgccg gacctcttgg cccggttcaa aggaagtgaa
      721 ccgataggcc tcttccaacc ccgcccggga cccgttgcgc gaatcctcgg gccggggcaa
      781 ccgttcggtc acccccaccg tttgaccggg gttgatcccc caggcgattt ggggttccaa
      841 ttcggcggcg ttcaggttga cgttgtccgc aaacaacgcg tccgggtccg accccatgga
      901 aagccaccac gccgcggcgc ggtcgaaatc ctttcccgcc gggacataag gccggccttt
      961 gagaaactcg acggtggtcc ggtccggatt gatgtaaccc acccgcgccc cgccctcaat
     1021 ggccatgttg cagaccgtca ggcgctcctc catcgacatg cgctcgatgg tgtcgccggc
     1081 gaattcgtac gcgaacccga tcccgccgtt gacgcccagc gtccggacga tcgccaaaat
     1141 cacgtccttc gcgtagaccc cggcggcgag gtttcccgaa acgcgaacgc ggcgcacctt
     1201 gggccgcgcc agcgccaggg tttgcgtcgc cagaatatcg cggatttggg tggtccccac
     1261 gccgaaggcg atggcgccga aggctccgtg ggtcgacgtg tgcgaatcgc cgcaggcgac
     1321 ggtcaagccc ggctgcgtca acccctgctc gggccccacg acgtggacga tgccctgctc
     1381 ccccgaggcg aaatcaaaaa accggacccc gaattcccgg caattgcgct ccaacgccac
     1441 catcatttcc tccgccaggc cgtccaggaa gggacggccc tgttggtccg tcgggacgat
     1501 gtgatccacc gtggcgaagg tccgtcccgg gtaggccacc cgaaggccct tctcccgaag
     1561 catctgaaag gcctgcgggc tcgtgacttc gtgcacgagg tgcaaaccga tgaacaattg
     1621 cgtttgaccg ttcggcagct cccggacggt gtgcagatcc caaaccttat cgagcagtgt
     1681 cttgcccatg tcacattcct tcgttctttt tggcggccgc gcggcccgag gcccgcgccg
     1741 acagaatttt attgagcgcg ttcaaatacg ccttggcgct cgcctccacg atgtcggtgg
     1801 aagtgccctt gccggtga
//
LOCUS       DAOQLS010000463         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46323_length_1818_cov_3.865003, whole genome shotgun sequence.
ACCESSION   DAOQLS010000463 DAOQLS010000000
VERSION     DAOQLS010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46323_length_1818_cov_3.865003"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1818
                     /locus_tag="PK362_08480"
     CDS             <1..>1818
                     /locus_tag="PK362_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNC74899.1"
                     /translation="YGRDKPGEYLKLMRDRFDENSILQELTGQDFARFDARYREWLTD
                     YYAEQAIGATEPESDGVRATPTDGLPVFNSGPALSPDGRRLAYVSDRGGRHEVIVRRL
                     DTGEEITVAGLEGARTENLHSQNRALAFSPDGRWIAFFGEKEQRDYLYLFDVERRRLR
                     RVRAPLAEMRSPTFSPDGARLAVSALVVGRRDLFEISRAGRVLRRLTQTPEDDDQPAY
                     SPDGRRLVFSREVPAGVDAPPDRQLVLWDLEAGAAAAETRLPGRETEPVFGPEGKDVF
                     FVGEGTSAIRNLYRWNPDSGAVSRLTRVVGGNFSPVFSRDGGVSYFASYRDQSMHLYR
                     AAPDLWTRPGEPAASAAGAPPPPPPGPVAEARPYRWKASTDLFFPVVYYSSEGGLFAA
                     ALWQSSEWLGDHDLRLVFQYASGDRLLDYTAEYAYRRFRPEFFLAASGQSFYNDVDRT
                     DREREAVRTLGARYPLDRFHRLEGAVSLLHRDRTFSLSPSRDFQEDENTFSAGWTRDT
                     AAGRYLGVTRGSRLSLEEKWTSGALGTDRRYRTHRAEHHVFFPTGGESALAFRTRAGA
                     SVGHEPERLRLGGADRLRGYPRRGDDVRSTRFLLANAEWR"
BASE COUNT          276 a          574 c          630 g          338 t
ORIGIN      
        1 atacggccgg gacaaaccgg gggaatatct gaagttgatg cgggaccggt tcgatgaaaa
       61 ttccatcctg caggaattga ccggccagga cttcgcccgt tttgacgccc gctaccggga
      121 atggctgacg gattattacg ccgagcaggc gatcggcgcg accgaacccg agagcgatgg
      181 cgttcgggcg acgccgacgg acgggctgcc cgtgttcaac agcggaccgg cgctttcgcc
      241 cgacggccgt cggctggcct acgtgtccga ccggggcggt cgccacgagg tcatcgtgcg
      301 ccggttggac acgggggagg aaatcacggt cgcgggcctg gagggggcgc ggacggagaa
      361 tcttcattcc caaaaccggg ccctggcttt ttcgcccgac gggcggtgga tcgctttttt
      421 cggcgaaaag gaacaacggg attatttgta tctcttcgac gtcgagcggc gccgtcttcg
      481 ccgggttcgc gcgcccctgg cggaaatgcg gtcgccgact ttttcaccgg acggcgcccg
      541 cctggcggtc tcggctctgg tggtggggcg gcgggacctt tttgaaatat cccgggcggg
      601 ccgcgttctc cgacgtctga ctcaaacccc ggaagacgac gaccagccgg cttacagccc
      661 ggacggacgt cgactcgttt tttcccggga ggtgccggcc ggggtggacg ccccgccgga
      721 tcggcaattg gtgctttggg acctggaagc cggagcggcc gcggctgaaa cccgcctgcc
      781 cggccgagag accgagccgg ttttcgggcc ggagggaaag gacgtttttt tcgtggggga
      841 gggaacctcc gccattcgaa atctctaccg ctggaatccc gattccggcg ccgtgtcccg
      901 gttgacccgg gtggtgggag gaaacttctc gcccgtgttc tcccgggacg gcggggtttc
      961 ctatttcgcg agctaccggg atcaatccat gcacctttat cgggcggcgc cggatctgtg
     1021 gacccgaccg ggggaaccgg ccgcttcggc ggcgggcgct ccgccgcccc cgccgcccgg
     1081 accggtggcc gaagcccgtc cctaccggtg gaaggcctcc accgatttgt ttttcccggt
     1141 ggtgtattac tcctccgagg gcgggctttt cgcggcggcc ctctggcaat cctccgagtg
     1201 gctgggcgac cacgatctgc gcctcgtctt tcaatacgcc agcggggacc gtttgttgga
     1261 ctacaccgcc gaatacgcct accgccgttt ccgtccggaa ttctttctcg cggcctcggg
     1321 gcaaagcttt tacaacgacg tcgatcggac cgatcgggaa cgggaagcgg tgcggaccct
     1381 cggggcccgc tatcccctgg accggttcca ccgactggaa ggggccgttt cgttgctgca
     1441 ccgggaccgg acgttttccc tttccccgag ccgggatttt caggaggacg aaaacacttt
     1501 ttcagcgggt tggacccggg acacggccgc gggtcgttat ttgggtgtca cgcgcggttc
     1561 ccgcctctcg ttggaagaga aatggacttc gggggccctg ggcacggacc ggcgctaccg
     1621 gacccaccgg gcggagcacc acgtcttttt ccccacgggg ggggaaagcg cgttggcttt
     1681 tcgaacgcgg gcgggggcga gcgtcgggca cgagccggaa cgccttcgct tgggcggggc
     1741 agaccgactt cggggctacc cccgccgggg cgacgacgtc cgatcaacgc ggtttctgct
     1801 ggccaacgcc gaatggcg
//
LOCUS       DAOQLS010000464         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46424_length_1816_cov_5.047132, whole genome shotgun sequence.
ACCESSION   DAOQLS010000464 DAOQLS010000000
VERSION     DAOQLS010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46424_length_1816_cov_5.047132"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK362_08485"
     CDS             complement(<1..448)
                     /locus_tag="PK362_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNC74900.1"
                     /translation="MKKNVLIIGAGAVAHVAAHKAAQHNDVLGDICLASRRVEKCDQI
                     IDSVRRKKSLKDPSKKIYSRAVDALDIPALTKLIKDTRSEIVINLGTAYINMSVLEAC
                     LAAGVTYMDTAIHEDPAKVCEDPPWYANYEWKRKDRCKEKGVTAILG"
     gene            complement(466..1614)
                     /gene="nspC"
                     /locus_tag="PK362_08490"
     CDS             complement(466..1614)
                     /gene="nspC"
                     /locus_tag="PK362_08490"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNC74901.1"
                     /translation="MTLAEPSIEQLLTETRSRPTPFYLLDETRLLRNLRRITRLRERS
                     GAKAVLALKCFSTWSVFPLMREHLDGTTSSSLFEARLGHETFGKETHAYSVAFSDADI
                     EAVRGFATKVIFNSTSQLLRYHDRVKHLPVGLRINPGVSHSHFDLADPARPRCRLGAT
                     SAADIDAVADRLSGVMFHCQCENDDFEKFKFLLDRIAADYGPLLKRLDWVSLGGGIYF
                     TKEGYPLDDLAEALRDFAARFDVQVYLEPGEAAITRTAYLVTSVLDLVRNEVDIAVVD
                     SAVEPHMLDLLIYRTDAKMDLPSGGRFPYTVAGKTCLAGDVFGTFPFPAPLAVGDRVA
                     FADAAGYTMVKKNWFNGVAMPSIVVRRLDGRLDAVRDFTYDDYRSQLS"
BASE COUNT          326 a          546 c          604 g          340 t
ORIGIN      
        1 cgcccaaaat ggccgtcacg cccttttcct tgcaccggtc cttccgcttc cattcgtagt
       61 tggcgtacca gggcgggtct tcgcagactt tggcggggtc ttcgtgaatg gccgtgtcca
      121 tgtaggtcac gccggcggcc aggcaggcct ccaggacgga catgttgatg tacgccgtgc
      181 caaggttgat gacgatttcc gagcgcgtgt ccttgatcag tttcgtgagg gccggaatgt
      241 ccagggcgtc gaccgcccgg gaataaattt ttttcgaagg gtccttgagg ctctttttcc
      301 gccggacgct gtcgatgatc tggtcgcact tctcgacccg gcgggaggcc aggcagatgt
      361 ctcccaggac gtcgttgtgc tgggccgcct tgtgggccgc cacgtgggcc accgcgcccg
      421 cgccgatgat caaaacgttt ttcttcatga tgccctcacc ccctcttagg aaagctgcga
      481 tcgataatcg tcgtaggtga aatcccgaac ggcgtccagg cggccgtcca gccggcggac
      541 gacgatggac ggcatggcca cgccgttgaa ccaatttttc ttcaccatcg tgtagcccgc
      601 cgcgtccgcg aaggccacgc gatcgcccac ggccaagggc gccggaaatg gaaaggtccc
      661 gaacacgtcc ccggccaggc acgtcttgcc ggcgacggtg taaggaaaac gtccgccgga
      721 cggaagatcc atcttagcat ctgtccggta aatcagtaaa tcgagcatgt ggggttcgac
      781 cgcgctgtcg acgaccgcaa tgtcgacctc gttgcggacc aggtccagga cgctcgtcac
      841 cagatacgcc gtgcgggtga tggcggcctc gccgggctcc agatagacct gcacgtcgaa
      901 gcgcgccgcg aaatcccgca gggcttcggc caggtcgtcc aggggatagc cctctttcgt
      961 gaagtagatg ccgccgccca ggctcaccca atccagccgt ttcaggaggg gcccgtagtc
     1021 cgcggcgatc cggtccagga ggaatttgaa tttttcaaaa tcgtcgtttt cgcattggca
     1081 gtggaacatg acgccggaca atcgatcggc cacggcgtcg atgtccgccg cgctcgtcgc
     1141 cccgaggcgg caacggggcc gggccggatc ggccaggtcg aaatgggaat ggctcacgcc
     1201 ggggttgatg cggagaccca cggggaggtg cttgacgcgg tcgtggtagc ggaggagttg
     1261 ggacgtcgag ttgaaaatca ctttggtcgc gaaaccgcgg accgcctcga tgtccgcgtc
     1321 ggagaaggcc acgctgtagg cgtgggtctc cttcccgaag gtctcgtggc ccagccgggc
     1381 ctcgaagagc gagctggagg tcgtgccgtc cagatgctcc cgcatcaggg ggaacacgct
     1441 ccaggtggaa aagcacttga gggccagcac ggccttggcc ccggagcgtt cccgcaggcg
     1501 ggtgatccgc cggagatttc gaagcagacg ggtttcgtcc agcaggtaaa agggcgtcgg
     1561 ccgggaacgg gtctcggtca acagttgttc gatggagggc tcggcgaggg tcatggggac
     1621 ggattccgtc gtgcgcgacg acggacatta tatcaggttt gcccgagggc atgaaccgcg
     1681 ggcctcccga cattataaaa tggcggcgtc gcaccaccgc ccgctgggga ggatttatga
     1741 aacgcacgac ctggaacaag cttgttttga cgatggcggc cttggccgcg gtcgcgccct
     1801 cccgggcggc gaaccg
//
LOCUS       DAOQLS010000465         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46436_length_1816_cov_3.657013, whole genome shotgun sequence.
ACCESSION   DAOQLS010000465 DAOQLS010000000
VERSION     DAOQLS010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46436_length_1816_cov_3.657013"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK362_08495"
     CDS             <1..218
                     /locus_tag="PK362_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74902.1"
                     /translation="DGGDFLITGVLVEGAPKIDLMLVHESLASNRSDLTVGLVAFLLL
                     CGSGSYIIWAIRRKTGPSAGTFSKPSR"
     gene            264..851
                     /locus_tag="PK362_08500"
     CDS             264..851
                     /locus_tag="PK362_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNC74903.1"
                     /translation="MFRKSLLFAVALATLGSLRAADTYTIDNAHSSVGFAVKHLMVTT
                     TKGQFKDYTGTIQYDAKDISKSSVKVTIKTASINTDNNDRDNHLRSPDFFDATKNPDI
                     TFESTKIVKQGLGYEAAGKLTIKGVTKDVKIPFTINGPVKDPWGNTRIGVEGGLTINR
                     QDFGVSWSKTLDNGNLVVANDVKIDLVIEGIQAKK"
     gene            complement(922..1494)
                     /locus_tag="PK362_08505"
     CDS             complement(922..1494)
                     /locus_tag="PK362_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC74904.1"
                     /translation="MENLHSHSFTPRRRLFISASFQGKLLWGIAAATLLSFALLAVDY
                     YVFFGRNVPTSPWDPEMMWIFLKANRPLIIQLVVFVTVLCAITLVLSHRVAGPLYSLE
                     KSIKIVQEGNLVHRARFRKRDELSHLRDAFNAMLDAVHGKVLKDRDAVAAARREIEPL
                     LARPDLNPETKAQLERIKSHLESVTRGFTL"
     gene            1572..>1816
                     /locus_tag="PK362_08510"
     CDS             1572..>1816
                     /locus_tag="PK362_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNC74905.1"
                     /translation="MGRSEPEIVYFSDEEFAKRVVGDSIFKLWQAVDDLARLRPRRRP
                     RYRVTIFGSARARPGTLAYRHTGQLALALARMGCDIVT"
BASE COUNT          401 a          520 c          536 g          359 t
ORIGIN      
        1 tggacggcgg ggattttttg atcacggggg tgttggtcga aggggcgccc aaaatcgatc
       61 tgatgctggt gcacgaatcc ctcgcgtcca accgatccga tttgaccgtg ggcctggtgg
      121 ccttcctgct tttgtgcggg agcggaagtt atataatttg ggcgatccgc cgaaaaacgg
      181 gcccgtccgc gggaactttt tccaagccgt cgcgttaagt tagacgcaac ccctttaacg
      241 aatcccacac tcaaggaggc ttcatgtttc gcaaaagtct gcttttcgcc gtcgccttgg
      301 cgaccctcgg ttcgcttcgg gccgcggaca cctacaccat cgacaacgcc cattcgtcgg
      361 tcggttttgc cgtcaaacac ctcatggtga cgaccaccaa aggccagttc aaggattaca
      421 cgggcacgat tcaatacgac gcgaaggaca tctccaaatc ctccgtaaag gtcacgatca
      481 agacggcgtc gatcaacacg gacaacaacg atcgggacaa tcaccttcgc tcgccggatt
      541 tcttcgacgc gaccaagaac ccggacatca cctttgaaag caccaagatc gtcaaacagg
      601 gcctgggcta cgaagccgcg ggcaaattga cgatcaaagg cgtgacgaag gacgtcaaaa
      661 tccccttcac catcaacggc cccgtgaagg acccctgggg caacacccgc atcggcgtgg
      721 agggcggatt gaccatcaac cggcaggact tcggcgtgag ttggagcaaa accctggaca
      781 acggcaacct ggtggtcgcc aacgacgtga aaattgatct ggtgatcgaa ggtattcaag
      841 cgaagaaata actcctttca aaaacgcgac gggccggttc cgcctcggcg gaaccggccc
      901 gtttttttcc ggaatcgtgg tttacagcgt gaacccgcgg gtcacggatt ccaaatggga
      961 tttgatccgc tccagctggg ctttggtttc ggggttgagg tccggccggg ccagaagggg
     1021 ttcgatttcc cggcgggcgg cggccacggc gtcccggtct ttcaacacct tcccgtgcac
     1081 ggcgtccaac atggcgttga aggcgtcgcg caggtgggac aactcgtccc gttttcggaa
     1141 tcgggctcgg tggaccaggt ttccctcttg gacgattttt atcgattttt ccaggctgta
     1201 aagggggccc gcgacgcgat gggacaacac gagggtgatc gcgcacagaa ccgtcacaaa
     1261 gaccacgagt tggatgatca acggccgatt ggctttcaga aagatccaca tcatttcggg
     1321 gtcccagggg ctcgtgggca cgttccggcc aaagaagacg taataatcca ccgccagtag
     1381 agcgaacgaa agcaacgtgg cggcggcgat gccccaaaga agttttccct gaaagctcgc
     1441 gctgatgaaa aggcgccgcc gaggggtaaa agagtgtgaa tgaaggtttt ccatcgcgtt
     1501 gttccgcctt aaacgtatcc ccggaaggga acgctgtcaa cgggacaatc ccgaagagct
     1561 agaatagggg catgggccgg tccgaacccg aaatcgtcta cttctccgat gaggaattcg
     1621 ccaaacgcgt ggtgggcgat tccatattta aactgtggca ggccgtggac gatctcgccc
     1681 gcttgcgtcc tcgacgacgc ccccgctatc gggtcacgat tttcggttcc gcccgggcgc
     1741 gccccggcac cttggcctac cggcacacgg ggcagttggc gctggctttg gcgcgaatgg
     1801 ggtgcgacat cgtgac
//
LOCUS       DAOQLS010000466         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46762_length_1810_cov_3.908262, whole genome shotgun sequence.
ACCESSION   DAOQLS010000466 DAOQLS010000000
VERSION     DAOQLS010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46762_length_1810_cov_3.908262"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..>1810
                     /locus_tag="PK362_08515"
     CDS             151..>1810
                     /locus_tag="PK362_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74906.1"
                     /translation="MADTDGNRVVVFSPEGKFRWAFGARGDLPGNLSAPGGVAVAADG
                     SVAVADAGNKRAQFFSADGLLLDVWNVPGDKPGEPLAVAILPGRRLAVLCRGEGRVRQ
                     LGDGGVAAGEWAWAGSPARLSAIAGAPSAGLYVADEASGRIVEWGPAGEERNSFGSAG
                     TGPGQFRTVSALAAQGGQLAVLDQKMGRVQVFRLHGNGASDGFPAPVGRLAPARSVEI
                     PLAAEDVAARGTDLIALTAKKILRVADLGDRPGTPVEIKGETAWSGVRGVSGRDGGAL
                     LAEDGRGRAVAVGWDGKVSTAYGIDAKGKGRLKSVGAAAADGDGRIYVADPSAGKVSG
                     FGPDGIPLFSAGVSDPVDVAGLPKGAAVLNGERKTLVLLDERGKTVWESTAAFVNPVS
                     VATDLSGPQPLVYVLDEGVHRVSVWGGNRLLGQFGSSEILIRPRALTVDGAGRVWVAD
                     EKTVKGFRVSVRPAGVEGLTAKGGDGAIQLSWKPDGEGRAGSYRLEKSTRAAGGGTPV
                     GVVPSTTTAFIDEDIVPGLTYYYTLTPAARGAADDPRRPPERAAL"
BASE COUNT          279 a          564 c          668 g          299 t
ORIGIN      
        1 cacgtcgtcg tacgccgcga ccgaaagagc ggtcgccgtc aacaacaaaa gacaaacggg
       61 ttttttcatt gaaacacctc gaccgaaaaa ggcgccggca aactgaaggg tcccggggcg
      121 ctggcgctgg gtcccaaggg cgatttgtac gtggcggaca ccgacgggaa ccgggtggtc
      181 gttttttccc cggagggcaa attccgctgg gccttcggcg cccggggcga tttgcccgga
      241 aacctgagcg ctccgggcgg cgtggccgtc gccgcggacg ggtccgtggc cgtggccgac
      301 gcgggcaaca agcgggcgca attcttttcg gccgacggcc tgttgttgga tgtttggaac
      361 gtgcccgggg acaaacccgg cgaacccctg gccgtggcga ttttgcccgg ccgccgattg
      421 gccgttctct gccggggcga agggcgggtg cgtcagctgg gcgacggcgg cgtggccgcg
      481 ggcgaatggg cctgggcggg gtctcccgcc cgattatcgg cgatcgccgg ggctccgagc
      541 gccggactct acgtcgccga tgaagcctcc gggcgaatcg tcgaatgggg tcccgccggc
      601 gaggagcgaa acagtttcgg gtccgccggc acggggccgg gtcagtttcg aaccgtttcc
      661 gcgttggccg cccagggcgg tcaattggcg gttctcgatc aaaaaatggg gcgtgttcag
      721 gtttttcgac ttcacggcaa cggggccagc gacggattcc cggcgcccgt gggccgattg
      781 gccccggccc ggtcggttga aatccccttg gccgcggagg acgtggccgc ccggggaacc
      841 gatttgatcg cgttgaccgc caaaaaaatc ctccgggtcg cggatctggg cgatcgcccc
      901 ggaacgcccg tcgagatcaa aggcgaaacc gcctggtcgg gcgtgcgcgg ggtgtccggc
      961 cgggacgggg gcgccttgct cgccgaggac ggtcgcgggc gcgcggtcgc cgtggggtgg
     1021 gacggaaaag tgtcgacggc ctacggcatc gacgccaaag gcaagggtcg tttgaaaagc
     1081 gtgggcgcgg cggccgcgga cggcgacggc cgaatctacg tcgccgatcc gagcgccggc
     1141 aaagtttcgg ggttcggtcc cgacggcatt ccgctgtttt ccgccggcgt gtccgatccg
     1201 gtggacgtgg cgggattgcc gaagggcgcg gccgttctta acggcgagcg gaaaacgttg
     1261 gttctcctcg acgagcgagg gaagaccgtg tgggaatcca cggccgcttt cgtgaatccc
     1321 gtgtcggtgg ccaccgattt gtcgggtccc caacccctgg tgtacgtcct ggacgaaggg
     1381 gttcaccgtg tctcggtgtg gggcgggaac cggttgttgg gtcagttcgg atcctcggaa
     1441 attttaatcc gtccccgggc gttgacggtg gacggcgccg gccgggtgtg ggtggcggat
     1501 gaaaaaaccg tcaaagggtt ccgcgtttcg gtccgtcccg ccggcgtcga aggcctgacg
     1561 gccaaggggg gcgacggcgc cattcagctt tcctggaaac cggacggcga aggccgcgcc
     1621 ggttcctacc ggctggaaaa gtccacccgc gccgcgggcg ggggaacccc ggtcggcgtg
     1681 gtgccctcga ccacgacggc ctttatcgac gaagacatcg tgcccggtct cacctactat
     1741 tacaccctga cccccgcggc gcggggcgcc gccgacgacc cgcgccggcc cccagaacga
     1801 gccgctctcg
//
LOCUS       DAOQLS010000467         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46856_length_1808_cov_3.993725, whole genome shotgun sequence.
ACCESSION   DAOQLS010000467 DAOQLS010000000
VERSION     DAOQLS010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46856_length_1808_cov_3.993725"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PK362_08520"
     CDS             complement(<1..451)
                     /locus_tag="PK362_08520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC74907.1"
                     /translation="MTPPPDVRAFLETLGLDPALLDRLDLLAREFARTNRDINLVSYS
                     TDGELWWNHLLDSLCVLKDPEARSLASGRAIDVGSGGGFPGVPLALAKPGWSVTLLDS
                     IQKKLRAVEGFLPVLGASVVARLGRAEEIARVPDFREKFDVALCRAVG"
     gene            complement(471..>1808)
                     /locus_tag="PK362_08525"
     CDS             complement(471..>1808)
                     /locus_tag="PK362_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC74908.1"
                     /translation="FHQTPGGRIGERPSNGLSDALRALGFEVGRLKTGTPPRLDGATL
                     DYARMEPAPGDDPPIPFSHFSSDLKQKMLPCWLTRTTEKTRDIVRRNLERSPLYSGVI
                     TSTGPRYCPSIEDKIVKFAHHPSHHVFVEPEGYDTTEIYPNGISTSLPEDVQEDIVHS
                     IVGFEQAKILRYGYAVEYDFCPPTQLRPTLETKAVERLFFAGQINGTTGYEEAAGQGI
                     VAGINAALALRNEAPWTPRRDEAYIGVMIDDLVTKGTDEPYRLMTSRAEWRLHLRWDN
                     ADLRLMDHGRRVGLIPEPAYEAFVKYRNTLWSAVRAALPGDAESSFLGDLPPTLSAVG
                     SLPLLSPEEERRAQWSADHVRRGVELEKQYWGYLKRQSREIAKFRQLESRRIPEDLDY
                     DRLNGLLTEARQKLKKVRPSSLGQAARIPGVTPSDVGVLLVHVERRRREAAAR"
BASE COUNT          349 a          547 c          586 g          326 t
ORIGIN      
        1 gacccacggc gcgacagagc gccacatcga atttttcgcg aaaatccgga actcgagcga
       61 tctcttccgc ccgtcccagt cgagcgacca cggaagcccc caggaccgga aggaatccct
      121 ccaccgcccg taattttttt tggatggaat ccagcaaggt cacggaccac cccggcttgg
      181 ccaaggccag cggcacgccg ggaaatcctc cccccgaacc aacgtcgatg gcgcggcccg
      241 aggccaggga tcgcgcctcg ggatctttca gcacgcagag ggaatccaaa agatggttcc
      301 accacaactc accgtcggtc gaatacgaaa cgagattgat gtcgcggttt gtccgggcga
      361 attcccgggc cagaaggtcc aatcggtcga gcagggcggg gtcgagaccc agggtctcca
      421 aaaaagcgcg gacgtccggg ggcggggtca ccggggacct cgggaatcct tcaacgcgcc
      481 gcggcttccc gccgtcggcg ttcgacgtgc accagcaaca cgcccacgtc cgaaggagtc
      541 acgccgggga tgcgggccgc ctgccccagg gaagacgggc ggactttttt gagcttttgt
      601 cgggcttccg tcagcaaccc gttgagccgg tcgtaatcca aatcctccgg aatgcgccgg
      661 gattccaatt gccggaattt ggcgatttcc ctcgattggc gtttcaggta gccccaatat
      721 tgtttttcca attccacgcc ccttcgaacg tgatcggccg accattgggc ccgccgctcc
      781 tcttccggcg aaaggagggg aagcgagccc acggccgaaa gggtgggagg aaggtcgccc
      841 aaaaaagagg attccgcgtc ccctggaagg gccgcccgaa cggcggacca gagggtgttc
      901 cggtatttga cgaaggcctc gtaggcgggt tcgggaatca gcccgacccg gcgcccgtgg
      961 tccatgaggc gcaaatcggc gttgtcccat cgcaaatgaa gacgccactc ggcccgggac
     1021 gtcatgaggc ggtagggttc gtcggtgcct ttggtgacca aatcgtcgat catgacgccg
     1081 atgtaggcct cgtcccggcg cggtgtccaa ggggcttcgt tccgaagggc cagggccgcg
     1141 ttgatcccgg cgacgatccc ctggcccgcg gcctcttcgt agcccgtggt gccgttaatc
     1201 tgtccggcga aaaacaatcg ttcaacggcc ttcgtttcga gcgtcggccg caattgggtg
     1261 ggggggcaaa agtcgtattc caccgcgtaa ccgtaacgga gaattttggc ctgttcgaaa
     1321 cccacgatcg aatgaacgat gtcctcctgc acgtcctccg gaaggctcgt cgaaatgccg
     1381 ttggggtaaa tctccgtcgt gtcgtaccct tcgggctcca caaacacgtg gtgcgacggg
     1441 tggtgggcga acttgacgat tttgtcttcg atggacgggc aataccgcgg cccggtggag
     1501 gtgatgacgc cggaatacag gggggaccgc tccaggtttc gacgaacaat gtcccgggtt
     1561 ttttccgtgg tgcgcgtcaa ccagcaggga agcatttttt gtttcaaatc cgaggagaag
     1621 tgggaaaaag gaatgggcgg gtcgtcgccc ggggcgggct ccatgcgggc gtagtccagg
     1681 gtggccccgt ccaagcgggg cggggtcccc gtcttgaggc ggcccacttc aaaccccagg
     1741 gctcgaagag cgtcggaaag gccgttcgag gggcgctcgc cgattcgacc cccgggggtc
     1801 tggtgaaa
//
LOCUS       DAOQLS010000468         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46989_length_1805_cov_5.009143, whole genome shotgun sequence.
ACCESSION   DAOQLS010000468 DAOQLS010000000
VERSION     DAOQLS010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46989_length_1805_cov_5.009143"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK362_08530"
     CDS             complement(<1..268)
                     /locus_tag="PK362_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNC74909.1"
                     /translation="MFGKKDIDPAKMETIIGPDTRFQGNVRSKGYVRVDGEIDGSVSA
                     EGVIIGEKAVVTGDITAKMVFVGGKVTGNVTAAAALELQPKGQIR"
     gene            complement(268..1272)
                     /locus_tag="PK362_08535"
     CDS             complement(268..1272)
                     /locus_tag="PK362_08535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC74910.1"
                     /translation="MKQLANPLWRFTMYRLWRRWRKEFGRRLTIMVVPHGIARPRQIS
                     FSVPFIVFLFTLWTGLTSWAGFLASQQFDYWRAKANAHFLRLKVDYFSGQLTQSRAML
                     DEVKQLEGELRTLIGLGSRDAIIQAENSPQPLEGTSNQGTGGPSLADAQALQSLLEGK
                     TRDMSFEEISKEIQDLRAEAEVRLASVQSLSQRITEERALFRSTPNIWPTNGYLTSHF
                     GPRLSPTHGFEENHKGMDIAGPAGSPVRVTADGVVKLARWAGGYGKVVVVDHGFGYST
                     RYGHNRQLLVQAGERVRRGQIVALMGETGNATGPHCHYEVWYNGRAVNPGKFLKRPSS
                     "
     gene            1402..>1805
                     /locus_tag="PK362_08540"
     CDS             1402..>1805
                     /locus_tag="PK362_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74911.1"
                     /translation="MNRKPNPPRLLNAQRTDVRLRATLLARLQTIHLLILAEPEIADL
                     IRQVENDPLFSRLVYPQEAAWKIVRFQPHPRTRLAPSFYEAKEDVLPAGAPAEAVQLL
                     GERRDVLSLIQRMGQENFEKHFLRAESDGALEA"
BASE COUNT          331 a          544 c          553 g          377 t
ORIGIN      
        1 cccgaatctg gcctttgggc tgaagttcca gggcggccgc ggcggtcacg ttgcccgtca
       61 ctttgccacc gacgaaaacc attttggcgg tgatgtcccc ggtcacgacg gctttttcgc
      121 cgatgatgac cccttcggcg gacacgctcc cgtcgatttc accgtcgaca cgaacgtagc
      181 ctttggaccg gacattgccc tgaaagcggg tgtcggggcc aatgatggtt tccatcttgg
      241 cgggatcgat gtcttttttg ccgaacatca ggagctcggc cgtttgagga attttccggg
      301 attcacggcg cggccattat accacacttc gtaatggcaa tgggggccgg tggcgttgcc
      361 cgtttcgccc atcaaagcga cgatctgtcc ccggcgaacc cgctcgccgg cctgaaccag
      421 gagttggcgg ttgtggccgt aacgggtgga ataaccgaac ccgtggtcga ccaccaccac
      481 tttgccgtac ccgccggccc accgcgccaa tttgacgacg ccgtcggcgg tgacgcggac
      541 gggcgacccg gccggacccg caatgtccat gcctttgtgg ttttcctcaa agccgtgggt
      601 cggggaaagt cggggaccga agtgggaggt caggtagccg ttggtgggcc agatgttcgg
      661 ggtggagcga aacagggctc gctcctcggt gatgcgctgg ctcagggatt ggacgctggc
      721 cagccggact tccgcctcgg cccggaggtc ctgaatttcc ttggaaattt cctcgaaact
      781 catgtcccgg gtcttgccct ccaacaggct ttgcagggcc tgggcgtccg ccaggctggg
      841 accgcccgtc ccttgattgg aggtcccttc caggggttgg gggctgttct cggcctgaat
      901 gatggcgtcc cggctgccga ggccgatcag cgtgcgaagc tccccttcca attgcttgac
      961 ctcgtccagc atggcccgcg actgggtgag ctggccgcta aagtaatcga ccttcagtcg
     1021 gagaaagtgg gcgttggctt tggcgcgcca ataatcgaat tgctgggagg ccaaaaagcc
     1081 ggcccagctc gtcaatccgg tccaaagcgt gaacaaaaac acgatgaacg ggacggaaaa
     1141 ggagatttgc cgtggtcggg cgatcccgtg ggggaccacc ataatggtca ggcggcggcc
     1201 gaattccttc cgccatcggc gccaaaggcg gtacatcgtg aacctccaca gggggttggc
     1261 aagttgtttc acattcttat gtaacagaaa tgcccatttc tgtcaaggtt aattaaaaac
     1321 gcgcgttctt tcggcaaaat aggccttgaa agcgatttgt tcgcccctta cacttaattc
     1381 cgtagcaacg ggaggaccat tttgaaccgg aaacccaatc ccccgcgcct gttgaacgcc
     1441 cagcggaccg acgtccggct tcgggccacg ctgctggcgc gcttgcaaac cattcatctt
     1501 ctcatcctgg ccgagcccga aatcgccgat ttgattcgcc aggtggagaa cgaccctctt
     1561 ttctcccgct tggtctaccc ccaggaagcc gcttggaaga tcgttcggtt tcagccccat
     1621 ccccgcacgc gcctggcgcc gtctttttac gaggcgaagg aggacgtgct cccggctggc
     1681 gccccggccg aggccgtgca gcttttgggg gaacggcgcg atgtcctttc cctcatccag
     1741 cggatgggtc aggagaattt cgaaaaacat ttcctccggg cggaaagcga cggggccttg
     1801 gaggc
//
LOCUS       DAOQLS010000469         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_46991_length_1805_cov_4.870857, whole genome shotgun sequence.
ACCESSION   DAOQLS010000469 DAOQLS010000000
VERSION     DAOQLS010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46991_length_1805_cov_4.870857"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..>1805
                     /locus_tag="PK362_08545"
     CDS             26..>1805
                     /locus_tag="PK362_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74912.1"
                     /translation="MAQILQNHGSLRDVYISKQPRAPLVVHIQDLHDSLDAQRNTAAI
                     LEALSEARGLTLVGLEGAQGAFATQAFRNFPEEALARSLADYFLKEGLIGGAEYAGWT
                     APRPPTLWGVEDGRAYLENIESVRRSAKAQGRVGKILAQAHRLLTDLRDRKASAPFMA
                     LERHVEAYAEHREAVGPYVHYLLRQKGVDPLGYGNLRLLDDALAWESSIDFRAVESER
                     NRLLERLVHRLGSTELKTLMDESLRYRAGGLTYGEYYRGFQNRLTAAGLRLEDYPQLN
                     GYIRYVLLSEKINRVELLGELATLERRAQVRAAASPFERSLVDLGRRYRLLDKLTRHA
                     LTPADWRDFQVHRLDLIGFSEELNRVAAEAGSTERVESLAGTDLDPFEDFCAQALARN
                     AALVDSLLAKMESDHSREAVLVAGGFHTEGLTQRLREKGISYVVVTPRVNETLAMGSR
                     ALDVLARDPIPLERLFAGPTINIVTPRATAVESPGRYGRTLTGLAVALGLFLAADNAS
                     AAIAAQRLPDVAEVRVRAMENDGREIDLRWRNGDKTRLETRAAEGEAGGDRTPLAVAT
                     LEVNRNGLSEPRTIHLYAPADSPWKRG"
BASE COUNT          329 a          556 c          572 g          348 t
ORIGIN      
        1 cggaaccgcc gccctcccga aacaaatggc ccaaatactc caaaatcacg gttcgcttcg
       61 ggacgtctat atttcaaaac aaccgcgggc gcccctcgtt gtccacattc aagacttgca
      121 cgactccctc gacgcccaac ggaacaccgc ggccattttg gaagctttgt ccgaggcccg
      181 cgggctgacg ctcgtcggtt tggagggcgc ccagggcgcc tttgccaccc aggcttttcg
      241 gaattttccc gaggaagccc tggcccgttc cctcgccgac tatttcctga aggaaggcct
      301 catcggcggc gccgaatacg cggggtggac cgccccccgg ccgccgacgc tctggggcgt
      361 ggaggacggg cgcgcttacc tggaaaacat cgagtccgtc cggcggtccg caaaagccca
      421 ggggcgggtc ggaaaaatac tggcccaggc tcaccgcctg ctgaccgacc ttcgcgaccg
      481 aaaagcctcg gcgccgttca tggccctgga aagacacgtc gaagcctacg cggaacaccg
      541 ggaggcggtc gggccctacg tgcattatct gttgcggcaa aaaggcgtcg atcccctcgg
      601 ctacggaaat ctgcggcttt tggacgacgc gttggcctgg gaatcctcca tcgattttcg
      661 agcggtcgag tcggaacgca accgccttct cgaaaggttg gttcatcgat tgggttcgac
      721 ggaattgaaa accttgatgg acgaaagcct tcgttatcgc gccggcggtt tgacctatgg
      781 cgaatattac cggggattcc agaaccgttt aacggccgcc ggtttgcgtc tggaggatta
      841 cccgcaactg aacggctata ttcgttacgt tctcctttcc gagaaaatca atcgggtgga
      901 attgctgggg gaattggcga cgttggagcg ccgcgcccag gttcgggccg cggccagccc
      961 ttttgaacga tcgctggtgg atttgggtcg tcgttaccgg ttgctggaca aattgacccg
     1021 gcacgccctt accccggcgg attggcgcga ttttcaagtg catcgcttgg acctgatcgg
     1081 tttttccgag gagcttaacc gcgtggcggc ggaggccggg tcgaccgaac gcgtcgaatc
     1141 cctggcggga accgatctgg atccctttga ggatttttgc gcccaagccc tcgcgcgaaa
     1201 cgcggcgctc gtggattcct tgttggccaa aatggaatcc gaccattccc gggaagccgt
     1261 cttggtggcg ggggggtttc acacggaagg cctgactcaa cgccttcggg aaaaagggat
     1321 ctcctacgtc gtcgttactc ctcgggtgaa cgaaaccttg gccatgggat cccgcgccct
     1381 ggacgtcctg gcccgggatc cgattccctt ggaaaggctt tttgccggtc cgaccatcaa
     1441 cattgtgacc ccccgggcga ccgcggtgga atcccccggg cgttacggcc ggaccttgac
     1501 ggggttggcc gtcgcgctgg ggcttttcct ggcggcggac aacgcttccg ccgcgatcgc
     1561 ggcccaacga ttgccggacg tggcggaggt tcgggttcgc gcgatggaga acgacgggcg
     1621 ggaaatcgac cttcgttggc gcaacgggga caaaacccgc ttggaaacgc gcgcggcgga
     1681 aggcgaggcc ggcggggatc ggacgccgtt ggcggtcgca acgttggaag tgaaccggaa
     1741 cggcctttcg gaaccccgta cgatccacct ttacgccccc gccgactccc cctggaaacg
     1801 cgggg
//
LOCUS       DAOQLS010000470         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47173_length_1802_cov_3.105896, whole genome shotgun sequence.
ACCESSION   DAOQLS010000470 DAOQLS010000000
VERSION     DAOQLS010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47173_length_1802_cov_3.105896"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PK362_08550"
     CDS             complement(<1..421)
                     /locus_tag="PK362_08550"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74913.1"
                     /translation="MKSRPLPLWTGPLATAVLYVFCYPRFNAGGGLGWVVLVPFLWAV
                     PRWPLKKAVRAGGAAGLLAHLGTLYWIYPTCRWGGVGHVVSALALLSLSAYLAVYWAA
                     FAAAVKMADGMKIARPFFLAAAWVALEYARAHLLTGFP"
     gene            complement(435..>1802)
                     /locus_tag="PK362_08555"
     CDS             complement(435..>1802)
                     /locus_tag="PK362_08555"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNC74914.1"
                     /translation="VLLAKMNVDAKGWAKATEKQAIAALNALLDQPDLDKALGIAEPP
                     AALSAELRDNVARARSAGQDVDTDTLRAYNRALLESLLPENILARERRGGLHILGTER
                     HESRRIDNQLRGRAGRQGDPGSSRFYLALDDELMRLFGSERMQAWMGRLGMQEGEDIQ
                     HPWVNKAIESAQTKVEAMNFDIRKQLLDFDNVMNKQREAVYKLRNAILDGLDLTDRLK
                     TMMVESLEEKLALWAPEKAHSDEWDFQSLKAWLSRGFGVEREFKSEVYPSSSALLAEL
                     TEVLEEAFQKRASTLGADLFRQMGRFILLQVMDTAWVDHLTYLEQLRKGIFLRAYGQK
                     DPLIEFQKEGFALFEGMMQRIREETLEYMFHLEAAPQAAPAERVMMLEKPETPVVTGE
                     IPRSEPARAVRPVPLIATARPNGNGKNPPAAPAAAALPTVKTIGRNDPCPCGSGKKYK
                     KCCGK"
BASE COUNT          318 a          556 c          599 g          329 t
ORIGIN      
        1 aggggaaacc cgtcaggaga tgggcgcggg cgtattccag cgcgacccag gcggcggcga
       61 ggaaaaaggg gcgggcgatt ttcatcccgt cggccatttt gaccgcggcc gcgaaagccg
      121 cccaataaac ggccaaatag gcggagagcg acagaagcgc cagggcgctc acgacgtgcc
      181 ccacgccgcc ccagcggcag gtgggataaa tccaatacag cgtgccgaga tgggccagga
      241 gaccggccgc gccgccggcc cgaaccgctt ttttcagggg ccatcggggc acggcccaga
      301 ggaagggcac aagaacgacc caccccagtc cgccgccggc gttgaaacgg ggatagcaaa
      361 agacgtagag gaccgcggtg gcgagcggcc cggtccaaag gggaagggga cgggatttca
      421 acggagggcg gggattattt gccgcaacac ttcttgtact tcttcccgct gccgcagggg
      481 caagggtcgt tgcggccgat ggttttcacc gtgggaaggg cggcggccgc gggggcggcc
      541 ggcgggttct ttccgttccc gttgggccgg gcggtggcga tcaagggcac cgggcgaaca
      601 gcgcgcgccg gctccgatcg agggatctcg cccgtcacga cgggggtttc gggtttttcc
      661 aacatcatca cccgttcggc cggggcggcc tggggcgcgg cttccaaatg gaacatgtat
      721 tccagggtct cttcgcggat tcgctgcatc atgccttcga agagggcgaa cccttctttt
      781 tggaattcga tcaagggatc cttctgcccg taggcccgaa ggaagatgcc cttccgcagc
      841 tgttccaaat aggtcaagtg gtccacccag gcggtgtcca tgacttggag gaggatgaac
      901 cgccccattt ggcggaacag gtcggcgccc agggtcgagg cgcgtttttg gaacgcctct
      961 tccaacacct cggtcagctc cgccaggagg gcggaggagg acgggtaaac ctcggacttg
     1021 aattcccgct ccaccccgaa cccccgggag agccaggcct tcaggctttg gaaatcccac
     1081 tcgtcggaat gggccttttc cggcgcccaa agggccagct tttcctcgag ggattccacc
     1141 atcatggttt tcagccggtc cgtcaggtcc aagccgtcca aaatcgcgtt ccgcagtttg
     1201 tagacggcct cgcgctgctt gttcatgacg ttgtcgaaat ccaacaactg tttgcggatg
     1261 tcgaagttca tggcctcgac tttggtttgg gcgctctcga tggccttgtt cacccagggg
     1321 tgctggatgt cctcgccctc ctgcatgccc agacgcccca tccaggcctg catccgctcg
     1381 gagccgaaga ggcgcatcaa ctcgtcgtcg agggccagat agaaacggga ggacccgggg
     1441 tcgccctgac gtcccgcgcg gccccgcagt tggttgtcga tgcgccggga ctcgtggcgc
     1501 tcggtgccga ggatgtgcaa cccgccccgt cgttcccggg ccaaaatgtt ttcggggagc
     1561 aaactttcca gaagcgcccg gttgtaagcc cggagcgtat cggtatcgac gtcttggcca
     1621 gcgcttcgcg cccgggcgac gttgtcccga agttcggcgc tcaaagcggc gggcggctcg
     1681 gcgatcccca gggccttgtc caaatccggt tgatccaaca gggcgttcag ggccgcgatg
     1741 gcctgttttt ccgtggcctt ggcccagccc ttggcgtcga cgttcatttt ggccaggagg
     1801 ac
//
LOCUS       DAOQLS010000471         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47192_length_1802_cov_2.454493, whole genome shotgun sequence.
ACCESSION   DAOQLS010000471 DAOQLS010000000
VERSION     DAOQLS010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47192_length_1802_cov_2.454493"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK362_08560"
     CDS             <1..180
                     /locus_tag="PK362_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74915.1"
                     /translation="RYYFADAAAQQYMALVADENTFDRNLAEPAKKLARLLRNLVESF
                     IFVRGGSVREFRENS"
     gene            246..665
                     /locus_tag="PK362_08565"
     CDS             246..665
                     /locus_tag="PK362_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNC74916.1"
                     /translation="MNPYRPKELTLFLLRIVAGLLFLQAGGMKLFDWFGGVPAEFGGH
                     PKLFSLIGIGGVLEFFGGLLVLLGLFTRPAAFIVSGQMAVAYFMFHQPKGFWPIQNHG
                     EPAVLLCFIFLLFSAHGGGDWSLDAKLCCKKTVVPSK"
     gene            838..>1802
                     /locus_tag="PK362_08570"
     CDS             838..>1802
                     /locus_tag="PK362_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC74917.1"
                     /translation="MSSDESQYRQISEAPTNPRGKGAAKGFDVGDLYQGPAAEGGPAQ
                     SAGMALDEKLRQAYFWVANHAIINPFYDIEYNDAPPAVIAMGDSKSPVSLPTDQSYSS
                     FVLLPLLNFAVRKRCLLVGGPGRGKTASAVLMGVLAGYPLKELRRAILHGQPQMTIAD
                     LLGNPLPSALVEAKSADQIQISWRKWLSMRIKIIDEYNRIPTRTQSALLTVMADNYAE
                     MFDQTFECPEAAWYLTANDDAGGGTYQVIEALKDRIDVVVKALHFNSRFLKELLERIE
                     LNIRPEEVVPPEIIFTEKELDEAHRRILAVGVPPAVRRRLEFFVS"
BASE COUNT          369 a          559 c          506 g          368 t
ORIGIN      
        1 aggtattatt tcgcggatgc agcagcccag caatacatgg cacttgttgc ggatgaaaat
       61 accttcgata gaaatcttgc tgagccagca aaaaagcttg ctcgtctttt gaggaatttg
      121 gttgagagct tcattttcgt gcgcggtgga agcgttcgag aattcaggga aaatagttaa
      181 actaaaaact gaacaaaaat gttcgaactt gtccattcgc caaataagta attaaggagc
      241 cccccatgaa cccctaccgc cccaaagaac taacgctctt tctcttgcgc atcgtcgccg
      301 gcctactgtt tttacaagcc ggcggcatga aactgtttga ctggttcggc ggagtccccg
      361 ccgaattcgg cggccatccc aaactcttct ccctgatcgg catcggcggg gtgttggaat
      421 tcttcggcgg gctcctggtt ctattgggcc tcttcacccg cccggcggct ttcatcgtct
      481 cgggccagat ggcggtggcc tatttcatgt tccaccagcc caagggtttt tggcccattc
      541 agaatcacgg ggaaccggcg gtcctgctgt gcttcatctt ccttcttttc tccgcccacg
      601 gcggcggcga ctggagcctg gacgccaagc tctgctgtaa gaaaaccgtc gttccttcca
      661 agtaaccgtc cgttctttaa aaaaaggccc gcctccccaa ggggcgggcc ttttttgttt
      721 gctcttgaaa aagggacggg gggaccctcg caaaagggct ttttatcgtc tatgcttctt
      781 ggaacgcctg aaagaccccg gcgatgccgg ggagacatca gccgacccga ggcccgcatg
      841 agctccgacg aaagccaata ccgacagatt tccgaagcgc ccaccaaccc ccgggggaaa
      901 ggcgccgcca agggctttga cgtaggggac ctctatcagg gccccgccgc cgagggcggc
      961 ccggcacaat ccgcgggcat ggccctggac gagaaactgc gccaggccta tttttgggtg
     1021 gccaaccacg ccatcatcaa ccccttctac gacatcgaat acaacgacgc ccccccggcg
     1081 gtaatcgcga tgggggacag caaatccccg gtgtccctgc cgacggacca gagctattcc
     1141 agcttcgtcc tcctgcccct gttgaatttc gccgttcgga agcgttgcct gctggtgggc
     1201 gggccgggcc ggggcaaaac cgccagcgcg gttttgatgg gcgttctggc gggctatccg
     1261 ctcaaagaac ttcgccgggc cattctccac ggccagcccc aaatgaccat cgcggacctg
     1321 ttgggcaacc ccctgccctc ggccctggtc gaggccaaga gcgcggacca aatccaaatt
     1381 tcctggcgga agtggctctc catgcggatc aagatcatcg acgaatacaa ccgcatcccc
     1441 acccggaccc agtcggcgct tttgaccgtc atggcggaca actacgccga aatgttcgac
     1501 cagaccttcg aatgccccga ggccgcctgg tacctgacgg ccaacgacga cgcgggcggg
     1561 ggcacctacc aggtgatcga ggccctcaag gaccgcatcg acgtggtggt caaggccctc
     1621 catttcaaca gccggttttt gaaggaactt ttggagcgca tcgagctgaa catccgtccc
     1681 gaggaagtgg tgccgccgga gatcatcttc accgaaaagg aactggacga ggcccaccgg
     1741 cgcattttgg ccgtgggcgt gcccccggcg gtgcgccggc ggttggagtt cttcgtcagc
     1801 tg
//
LOCUS       DAOQLS010000472         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47273_length_1800_cov_3.786246, whole genome shotgun sequence.
ACCESSION   DAOQLS010000472 DAOQLS010000000
VERSION     DAOQLS010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47273_length_1800_cov_3.786246"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK362_08575"
     CDS             <1..389
                     /locus_tag="PK362_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74918.1"
                     /translation="RGDQMTYVHLNRPQFVVFGRKIEFDLRSRNWQLHVMKRLGFLRR
                     WLPQWHAPEKAFRDWYTGIVDGFQIFHDEAVYRQYVEALQVPAGVRGYRQIRAPKMDA
                     ARARAEEILGSLKLRRPAEPASARRP"
     gene            420..1013
                     /locus_tag="PK362_08580"
     CDS             420..1013
                     /locus_tag="PK362_08580"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNC74919.1"
                     /translation="MPSVITIRRAKIGDVRAMHKLLSVFAEKKELLPRAISELYENLQ
                     QFHVADDKGRVVGCCSLAVQWDNLAEVKALAVDPDFQGRGIGRKLLDACLKDAKTLGI
                     TRVFALTMKDGFFDKIGFSRVGKNDLPHKVWTECVRCPYFPDACVEIAMVKDLGGVAP
                     VPLFKAGDLPADGTTPTEPEGFPAARRARAGKKRSGS"
     gene            1048..1533
                     /gene="rplM"
                     /locus_tag="PK362_08585"
     CDS             1048..1533
                     /gene="rplM"
                     /locus_tag="PK362_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNC74920.1"
                     /translation="MPTTQTHHPKPNTVERRWFVVDADSVALGRLATLSAGLLRGKGK
                     ATFNTHQDLGDGVVVINAGKVALTGNKKTQKMDFRASGFRGGQTFTHYGKLLQEKPER
                     AVELAVYGMLPKNRLRDRFMRRLKVFRAAEGSKIYPGAIVVDTKKPKIRSGGPFALAS
                     K"
     gene            1554..>1800
                     /gene="rpsI"
                     /locus_tag="PK362_08590"
     CDS             1554..>1800
                     /gene="rpsI"
                     /locus_tag="PK362_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNC74921.1"
                     /translation="MTSETIEITRQNPLWATGRRKTAIARVRLIPGEGRVTVNGKSVE
                     TFFGGNERQKASALAPFRVSNALSTFDIIVSTTGGGIT"
BASE COUNT          362 a          600 c          529 g          309 t
ORIGIN      
        1 cccggggcga tcaaatgacc tacgtccacc tgaaccggcc ccagttcgtg gtcttcgggc
       61 ggaaaatcga gttcgatctg cgctcccgga actggcagct ccacgtcatg aaacggttgg
      121 gcttcctccg ccgatggctg ccccagtggc acgccccgga aaaggctttc cgggactggt
      181 acacgggcat cgtcgacggt ttccagatat ttcacgacga ggccgtttac cgccaatacg
      241 tcgaggccct gcaggttccg gcgggcgtcc gggggtaccg gcagatccgc gccccgaaaa
      301 tggacgcggc ccgggcccgg gccgaggaga tcctcggatc gctcaagctt cgccgcccgg
      361 cggagcccgc ctcggcccgc cgtccctagg ccacgggaag acgccgccaa aatggtaaaa
      421 tgccttccgt gatcaccatt cgacgggcca aaatcggcga cgtgcgcgcc atgcacaagc
      481 tcctctcggt gtttgccgag aaaaaggagc ttttgccccg ggccatctcc gagctctacg
      541 aaaaccttca gcagtttcac gtggccgacg acaagggccg ggtcgtgggg tgttgctccc
      601 tggccgttca atgggacaat ttggccgagg tgaaagccct ggccgtggac cccgattttc
      661 aggggcgcgg catcggccgg aagcttttgg acgcctgtct gaaggacgcg aaaaccctgg
      721 gcatcacccg ggtgttcgcc ctcaccatga aggacgggtt tttcgacaag atcggatttt
      781 cccgggtggg caaaaacgac ctgccccaca aggtctggac ggaatgcgtg cggtgcccct
      841 atttccccga cgcctgcgtt gaaatcgcca tggtcaagga cctgggcggc gtggcgccgg
      901 tcccgttgtt caaggccggc gacctgcccg ccgacggcac gaccccgacg gaacccgagg
      961 ggttccccgc cgcccgaaga gcccgcgccg ggaaaaaacg ctcgggctct tgaccaaatt
     1021 gaattaatgt tctaaaatcc tgcccctatg ccgacgacac aaacccatca ccccaaaccg
     1081 aacaccgtcg aacgccgctg gttcgtggtc gacgccgaca gcgtggccct gggccgcctg
     1141 gccactttga gcgcgggtct cctgcgcgga aaaggcaaag cgacgttcaa cacgcaccag
     1201 gacctgggcg acggcgtcgt ggtcatcaac gccggaaaag tcgccctcac cggcaacaag
     1261 aaaacccaga aaatggattt ccgcgcctcc ggtttccggg gcgggcaaac cttcacccat
     1321 tacggaaagc tgttgcagga aaaacccgag cgggcggtgg aattggccgt ttacggaatg
     1381 cttcccaaga atcgcctgcg cgaccgtttc atgcgccgcc tcaaggtgtt ccgggcggcc
     1441 gagggctcca agatttaccc cggcgccatc gtcgtggaca ccaaaaaacc caaaattcgc
     1501 tccggcggtc ccttcgccct ggcgagcaag taaccctttc tgaaaggccg atcatgacgt
     1561 ccgaaaccat cgaaatcacg cgccaaaatc cgctgtgggc cacgggccgc cgcaaaacgg
     1621 ccatcgcccg cgttcgcctc attcccggcg aaggtcgcgt gacggtcaac ggcaaaagcg
     1681 ttgaaacttt tttcggcggc aacgagcgtc aaaaggcttc ggccttggct cccttccggg
     1741 tctccaacgc cctttccacc ttcgacatca tcgtttcgac caccggcggc ggcatcaccg
//
LOCUS       DAOQLS010000473         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47418_length_1797_cov_3.933410, whole genome shotgun sequence.
ACCESSION   DAOQLS010000473 DAOQLS010000000
VERSION     DAOQLS010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47418_length_1797_cov_3.933410"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK362_08595"
     CDS             complement(<1..183)
                     /locus_tag="PK362_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNC74922.1"
                     /translation="MADRGAGGHPLTASADLFDWERQNAFARLSGDANPLHVDPAAAR
                     RLLFGRAVVHGVHGVLR"
     gene            complement(266..1561)
                     /locus_tag="PK362_08600"
     CDS             complement(266..1561)
                     /locus_tag="PK362_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNC74923.1"
                     /translation="MTSTTDPLGRDLYDLAKTLFPFPRSLTGNGVRRTLAEIQKHLPG
                     LAVHEVPSGTRAFDWTVPDEWNVEDAYVLDARGRRVIDWKKNNLHLVGYSVPVDATLS
                     REELDQHLHSLPNQPTAIPYVTSYYARRWGFCLSHRARRALKKGTYRAVIKSRLAPGS
                     MTYGELLLPGREPAEVLLSTYVCHPSMANNECSGPAVTTFLAKALAARTARRLSYRVV
                     FVPETIGAILYLSRNLEALRRSTVAGYVVTCVGDDRVYSFLPSRLGDTLADRMARHVL
                     KHRAPRARHYSYLQRGSDERQYGWPGVDLPVASIMRSKYHEYPEYHTSLDDLKFISPR
                     GLAGAYRVLQETLDGLESNGTFQTTVLGEPQLGPRGLYASLGTKKTDARAEDYLNLLA
                     YCDGTRDLLAVAEIIQRPLADCAQWAALLMEHGLLRRTA"
     gene            complement(1558..>1797)
                     /locus_tag="PK362_08605"
     CDS             complement(1558..>1797)
                     /locus_tag="PK362_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74924.1"
                     /translation="ADDLRGRGLLRSAPLGRGRGLRCRADLVAADWAERLRADPFYLL
                     TAESRRELEALQRRVGGRLTLENVEGRDAAPAENL"
BASE COUNT          301 a          592 c          627 g          277 t
ORIGIN      
        1 ccgcagaacg ccgtgcaccc cgtggaccac ggcccgcccg aaaagaagcc gccgggcggc
       61 ggcgggatcg acgtgcaggg ggttggcgtc cccggacagc cgggcgaagg cgttttgccg
      121 ttcccaatcg aaaaggtcgg cggacgcggt caagggatgg cctcccgctc cgcggtcggc
      181 caccgcttgc gcggggcatg gaccccgcgc aagcactggg cgctttttcc ccgggccaag
      241 gcccggggca tggggggaac ccctgtcacg cggtcctccg cagaaggccg tgctccatca
      301 ggagcgccgc ccattgggcg caatcggcca agggccgttg aatgatttcg gccacggcca
      361 gcaagtcgcg agtgccgtcg cagtaggcga ggaggttgag gtaatcctcc gcccgggcgt
      421 cggttttctt ggtgcccagg ctggcgtaga ggccccgggg ccccaactgg ggttcgccga
      481 gcaccgtcgt ttgaaacgtc ccgttggact cgaggccgtc cagggtttcc tgaagaacgc
      541 ggtacgcccc cgccaacccc cggggagaaa tgaatttcag atcgtccagg gaggtgtggt
      601 attcggggta ttcgtggtat ttgctccgca tgatggaggc cacgggcaaa tccaccccgg
      661 gccagccgta ctgccgctcg tcgctgcccc gttgcaaata agaatagtgt cgcgcacggg
      721 gcgcccggtg tttcagcacg tggcgcgcca tccggtcggc cagcgtgtcg ccgagacggg
      781 acgggaggaa cgaataaacg cggtcgtcgc ccacgcaggt cacgacgtag ccggcgacgg
      841 tggagcggcg cagggcctcg aggttccggc taagatagag aatggcgccg atggtttccg
      901 gcacgaagac cacccggtag gacaaccgcc gcgccgttcg ggcggccagg gccttcgcca
      961 ggaacgtcgt cacggccggc ccggaacatt cgttgttggc catggagggg tggcacacgt
     1021 aggtcgacaa caagacctcg gcgggctccc gcccggggag gagcagttcc ccgtaggtca
     1081 tggaacccgg cgccaggcgg gatttgatca cggctcggta tgtccctttt ttaagggccc
     1141 gacgcgcccg atgcgacagg caaaaccccc atcgccgggc gtaataggag gtcacgtacg
     1201 gaatggccgt cggttggttc gggagcgagt gcaggtgttg atccagctcc tcccgggaga
     1261 gcgtcgcatc caccggcacg gaatagccga ccaggtggag attgtttttc ttccagtcga
     1321 tcacccgccg accccgcgcg tccagcacgt aggcgtcctc cacgttccac tcgtcgggca
     1381 ccgtccagtc gaaggcccgg gtccccgagg gcacctcgtg caccgccagg ccgggcaaat
     1441 gtttttggat ctcggccagg gtgcgccgca ccccgtttcc ggtcaggctg cgcggaaagg
     1501 ggaaaagggt tttggccaga tcgtaaagat cgcggccgag ggggtccgtc gtcgatgtca
     1561 taggttttcg gcgggcgcgg cgtcgcgccc ctccacgttc tccaaggtca ggcggccgcc
     1621 cacgcgccgt tgaagcgcct ccaattcccg gcgggactcc gccgtcagca ggtaaaacgg
     1681 gtcggcccgc agccgctccg cccaatcggc cgccacgagg tcggcccggc accgaaggcc
     1741 ccgaccgcgg cccaggggcg cggagcgcag aagaccccgg ccccgcagat cgtcggc
//
LOCUS       DAOQLS010000474         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47498_length_1795_cov_5.117816, whole genome shotgun sequence.
ACCESSION   DAOQLS010000474 DAOQLS010000000
VERSION     DAOQLS010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47498_length_1795_cov_5.117816"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK362_08610"
     CDS             <1..255
                     /locus_tag="PK362_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNC74925.1"
                     /translation="EGNVPGRFYTTTACIACGSCEGLAPGLFRGLPSGQYVVVRQPRD
                     EKELAAAREAYQITFEPNGPGPCIVDTFDPENTPAELRGR"
     gene            301..>1795
                     /locus_tag="PK362_08615"
     CDS             301..>1795
                     /locus_tag="PK362_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74926.1"
                     /translation="MSALPKAYSTLTDRRDIDGLMDALRRAPAAHRRALIEAVFSIYQ
                     DIAMALAEGKLKWPRADLLFDRVARTLDRWWDPLDFEIRWDIRLMTLGMFPQAPRLLR
                     RKRAAVLRSLADRWFARRPRDPAVLRNRAEALWQQYRVDRNKASLRRLLDFHRKTTAA
                     RGPDAFWTQWLSHLSRAARGMPAAHRPILKMGENDFCRSAGGEWSAGRLNPFILAEAF
                     HELAHGADVPAGWRKERRFWLDRCLSLWPTDAETPQTLDRRGDVFRRAYEHYKDVRYL
                     IGALGCYQKQWEKSPEGALYSLIRTKTALAEGVRPVSENQYRRMILEQWPAVEKTARR
                     PASASLPLNLRPAASFSKWNSAAEFLEKNREVLLAEDPTTPWDRRRYEMSLAAEKAGE
                     GYYGTPYRNLVDAGARLGRWVEAEAWMIRFFLRHTIVVDGDFRAFARAPWARKRSPLA
                     QTLRSILDYYENFPSSYYDGGMPVDRLRRLPLPRLRRVLRDALDKARA"
BASE COUNT          298 a          586 c          567 g          344 t
ORIGIN      
        1 gaggggaatg ttcccgggcg cttctacacg accaccgcct gcatcgcctg cggttcctgc
       61 gaagggctgg cccccggcct ctttcggggg cttccctccg ggcagtacgt ggtggtccgg
      121 caaccccggg acgaaaagga gctggccgcc gcccgggagg cctaccaaat caccttcgag
      181 cccaacgggc cgggcccgtg catcgtcgac acgttcgacc ccgaaaacac cccggcggaa
      241 cttcgggggc gttaaggcgc cgtcgttgaa ttcctccgtt aaaaatccca tgatggtccc
      301 gtgagcgccc ttccgaaagc ctattccacc ctgaccgacc gccgcgacat cgacggtttg
      361 atggacgccc tccgccgggc cccggcggcc caccggaggg ccttgatcga ggccgttttc
      421 tcgatctatc aagacatcgc catggccctg gcggagggaa aattgaaatg gccccgggcc
      481 gaccttctgt tcgatcgggt ggctcggacc ctggatcggt ggtgggatcc attggatttt
      541 gaaatccgct gggacattcg ccttatgacg ttgggaatgt ttccccaggc tccccggctc
      601 ctgcgtcgaa aacgggccgc ggttctccgt tccttggcgg atcgttggtt cgcccgccgt
      661 ccccgcgacc ccgcggttct tcggaacagg gccgaagccc tttggcagca ataccgggtg
      721 gaccggaaca aggcgtcgct tcgccgcctc ctggatttcc accggaaaac cacggcggcc
      781 cggggccccg acgccttttg gacccaatgg ctctcccacc tgagccgggc cgcccggggg
      841 atgcccgccg cccatcggcc gatcctgaaa atgggcgaaa atgatttctg tcggtcggcc
      901 gggggggaat ggtccgcggg ccggttgaac cccttcatcc tggccgaggc gttccacgaa
      961 ttggcccatg gggccgatgt tcccgccggc tggcggaagg aacgccggtt ttggttggat
     1021 cggtgtctgt ccctttggcc cacggacgcc gaaacgcccc aaaccctgga tcgacggggg
     1081 gacgtgtttc gacgggcgta cgagcattac aaggatgtcc ggtatttgat cggcgccctg
     1141 gggtgttacc aaaaacagtg ggaaaaatct cccgaggggg ccctctattc tttgatccgg
     1201 accaaaaccg ctttggccga aggggtccgc cccgtcagtg aaaatcaata ccgccggatg
     1261 attttggagc aatggcccgc cgttgaaaaa acggcccggc ggccggcctc ggcctccctc
     1321 cccttgaatc tccggccggc ggcgtctttt tccaagtgga attcggccgc cgaatttctg
     1381 gaaaaaaatc gggaggtcct tctggccgag gacccgacga ccccctggga tcggcgccgc
     1441 tacgaaatga gcttggcggc cgaaaaagcg ggcgaagggt actacggcac gccctatcgg
     1501 aatttggtgg acgccggcgc ccggttgggc cggtgggtcg aggccgaggc ctggatgatc
     1561 cgctttttcc ttcgacacac gatcgtggtg gacggcgatt tccgggcctt tgcccgggcg
     1621 ccctgggccc ggaagcgatc gcccctggcc caaacgcttc gttcgattct ggattattac
     1681 gaaaatttcc cttcgtccta ttacgatggg ggaatgccgg tggaccggct tcgtcgcctg
     1741 ccccttcccc ggcttcggcg cgtccttcgg gacgcgctgg acaaagcccg cgccc
//
LOCUS       DAOQLS010000475         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47570_length_1794_cov_2.658999, whole genome shotgun sequence.
ACCESSION   DAOQLS010000475 DAOQLS010000000
VERSION     DAOQLS010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47570_length_1794_cov_2.658999"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1794
                     /locus_tag="PK362_08620"
     CDS             <1..>1794
                     /locus_tag="PK362_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNC74927.1"
                     /translation="PVLNAPVPNLSWKNRLVIVLGGLAGTRKEAVARSLASRLGLRYL
                     RAGFLMRIVLYELLTRYRGTDLSNNERVARYARSILAEVDLTQEPVRVRGVSTAEPDA
                     EGDTYRDRVRALIDRSPENTLLLDRLAGLPGVQESLVEKINATLRVARSQNSYNGVVL
                     VVTRPALKSGGDPAVVPFFLHAEDALRAQRTGVDPRRLTERDRASGVVGLEGEFLPAA
                     RVDANRSVREVVKDISDRLQGAYQTEAGFYREFVETHGLKGGVGADIATSSHFEPALG
                     LRAGGLSTVYAVDFLNGAPKQIEEGVWSINGDATRLSATPEFGREKLDLLVYNDSLQY
                     IVDPHYWHNAAYVRENPGAWDGDDGFSLVQDLLDQSMKEAWATLRPGGRILITWTAVR
                     PSPSGRVAPLAGRDVRELLAFAGFTDIEPRELAPGRFVVTAVKPHPPSVGRVQSVFNA
                     ALTGLRTRGPNGRKLYLFLTVAVVPWLEELVFRQWVFGDASLLPLFQWFVAVPVFVLA
                     HRILHRESWVETFSRILPAVLFSAAFLAAPGQGYNVLLHALWNAAVLALGRGPLMSVA
                     GGAPAPLYDFYHRIFHRRPLRDVFDQAARRVG"
BASE COUNT          268 a          586 c          577 g          363 t
ORIGIN      
        1 ccggtactga acgcgcccgt tcccaacctt tcctggaaaa accgcctcgt catcgtcctg
       61 ggaggtctcg ccgggacccg aaaggaggcg gtggcccgat ccctggcgag tcggctgggc
      121 ctgcgttatc tgcgggcggg gtttttaatg cggatcgtcc tctacgaatt gctcacccgt
      181 taccgcggca cggacctttc caacaacgag cgcgtcgccc gctacgcccg gtccattttg
      241 gcggaagtgg atttgaccca ggaaccggtg cgggtgcggg gggtcagcac cgccgaaccc
      301 gacgcggaag gcgacacgta ccgcgaccgg gtgcgggccc tcattgatcg ttccccggaa
      361 aacacgcttc tgttggatcg tctcgccggc cttcccggcg ttcaagagtc gttggtcgaa
      421 aaaatcaacg ccacgttgcg ggtggcgcgg tcccagaatt catacaacgg cgtggtcctc
      481 gtcgtcaccc gccccgcctt gaaatccggc ggggaccccg ccgttgttcc ctttttcctt
      541 cacgcggaag acgccctgcg ggcccaacga accggcgtgg acccccggcg gttgaccgag
      601 cgggaccggg cctcgggcgt tgtcggactg gagggggaat tccttcccgc cgcccgggtc
      661 gacgccaatc ggtcggtccg ggaggtcgtt aaggacattt ccgatcggct tcaaggggcc
      721 tatcagaccg aggcggggtt ttaccgggaa tttgtcgaaa cccacggctt gaaaggcggg
      781 gtcggtgccg acatcgccac ctcgtcccat ttcgaaccgg cgctcggtct tcgggccggt
      841 gggctatcga cggtttacgc cgttgatttt ctgaacgggg ccccgaaaca aatcgaagag
      901 ggcgtttggt cgatcaacgg cgacgccacg cgcttgagcg ccaccccgga gttcggccgg
      961 gaaaaactgg acctgctggt ctacaacgat tccctgcaat acatcgtcga tccccactat
     1021 tggcacaacg cggcctacgt tcgggaaaac cccggagcct gggacggcga cgacggtttc
     1081 tcgctcgttc aagacctgtt ggatcaatcc atgaaggaag cctgggccac ccttcgaccc
     1141 ggcggtcgga ttttaatcac gtggacggcg gttcgcccgt cgcccagcgg gcgggtggcg
     1201 cctctggccg gtcgcgacgt tcgggaactg ctggcgttcg ccgggttcac cgacatcgaa
     1261 ccgcgggagc tggcgcccgg acgcttcgtc gtcaccgcgg tcaaacccca ccccccgtcg
     1321 gtggggcggg ttcaatcggt tttcaacgcg gcgttgaccg gcctacggac ccggggcccg
     1381 aacggacgaa agctttattt gtttctcacc gtggccgtcg tcccttggct ggaggagttg
     1441 gtcttccgtc agtgggtctt cggcgacgcg tccctcctgc cgttgtttca atggttcgtg
     1501 gccgtcccgg tgtttgtgtt ggcccatcgg atcctccatc gggaatcctg ggtggaaacc
     1561 ttttcgcgga tccttccggc ggtgctgttt tcggcggcct ttctggccgc cccgggccag
     1621 ggctacaacg tgctgctcca cgctctctgg aacgccgcgg tgttggccct cggccggggt
     1681 ccgttgatgt cggtggcggg cggcgccccg gcgccgctct acgattttta ccaccggatc
     1741 ttccaccgcc gtccgcttcg ggacgttttc gaccaagcgg cccgtcgcgt cggc
//
LOCUS       DAOQLS010000476         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47683_length_1791_cov_4.864631, whole genome shotgun sequence.
ACCESSION   DAOQLS010000476 DAOQLS010000000
VERSION     DAOQLS010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47683_length_1791_cov_4.864631"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PK362_08625"
     CDS             <1..599
                     /locus_tag="PK362_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNC74928.1"
                     /translation="NALAAAGGGLALGLSLTEVVQGLESVVEVPGRLERVTENPPGDP
                     TPSRAGLEVFVDYAHTDDALKNVLQTLRPLTTGRLIVLFGCGGDRDKTKRPRMGEVAA
                     RLADRVIVTSDNPRSEDPRQIAREVEAGVRRVAGRDYEVVVERGEAIARAIAAAGRGD
                     IVLLAGKGHETYQIFADGPVPFDDRSEARRRLAERVSR"
     gene            600..>1791
                     /gene="murF"
                     /locus_tag="PK362_08630"
     CDS             600..>1791
                     /gene="murF"
                     /locus_tag="PK362_08630"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNC74929.1"
                     /translation="MLPYAWTDLARWAGGQLTHAGTSPAARFVVDSRAVEPGDVFVAL
                     RGARQDGHAFLPEVFSRGASGCLVERTPPSVPDGVSVIRVPDALRALQSLARTHREKM
                     TSTVIGITGSNGKTTTKEMLSHVLRAAGKRVLSTRGNLNSQIGLPLMMLEMSKSHTHA
                     VLEMGASARGDIARLCELARPTVGVLTGIGRAHLEFFGSLAGVADAKWELVEALPSGG
                     VAFLNADDPLLMDRKKSARCSVVTFGRSPGADVRAENIRQEPRVVFDLVMAGARRPVQ
                     LPVPGIFNVTNALAAAAVALWERVPLPSLTSALESFAPPPGRMEVRRRKDGTLFVLDA
                     YNANPDSMAAGLRSFVDAYPERPRYAVLGSMLELGPHAEPEHRALGAGLAGLSFTAVY
                     FVGAE"
BASE COUNT          273 a          590 c          613 g          315 t
ORIGIN      
        1 acaacgccct cgccgccgcg ggcggcgggt tggccctggg actttccttg acggaggtgg
       61 tccaagggtt ggaatcggtg gtggaggtcc ccggacgcct ggaacgcgtc accgaaaacc
      121 cccccggcga tccgacgccg agccgcgcgg ggttggaggt gttcgtcgat tacgcccaca
      181 cggacgacgc ccttaaaaac gttttgcaaa ccctccgccc cctaacaacg ggacggttga
      241 tcgtcctctt cggctgcggc ggtgaccggg acaagaccaa gcggccgcgc atgggcgaag
      301 tcgccgcccg gttggcggac cgcgtcatcg tgacctccga caatccccgg tccgaagatc
      361 cccgtcaaat cgcccgcgaa gtggaggcgg gggttcggcg ggttgccggc cgggactacg
      421 aggtcgtggt ggagcggggc gaggccatcg cccgggccat cgccgccgcg ggccgggggg
      481 acatcgttct tctggcgggc aagggccacg agacctacca gattttcgcc gacggcccgg
      541 tgccctttga cgaccggtcc gaggcgcgcc gtcgattggc ggagcgggtg tcccgctaga
      601 tgcttcccta cgcttggacc gatttggccc gctgggcggg cggccaattg acgcacgccg
      661 gaacgtcgcc cgcggcccgg tttgtcgtcg attcccgggc cgtggagccc ggcgacgtat
      721 tcgtcgccct tcgcggggcc cgccaggacg gccacgcgtt tctgcccgag gttttttccc
      781 ggggagccag cggctgcctg gtggaacgga cccccccgtc ggttcccgac ggcgtttcgg
      841 tcatccgggt gcccgacgcc ttgagagccc ttcagtccct cgcccggacc caccgggaaa
      901 aaatgacctc cacggtgatt ggaatcaccg gatccaacgg aaaaaccacc accaaggaaa
      961 tgttgtccca cgtcctgcgc gcggccggaa agcgggtgtt gtccacccgg ggaaatttga
     1021 acagtcaaat cgggcttccg ctcatgatgt tggaaatgag caaatcgcac acccacgccg
     1081 tcctggaaat gggggcgtcg gcccggggcg acatcgcacg gctttgcgaa ttggcgcggc
     1141 ccaccgtcgg ggtcctgacc gggatcggcc gcgcccacct ggaatttttt ggaagcttgg
     1201 cgggcgtggc cgacgccaaa tgggagctgg tggaagccct tcccagcggg ggcgtggcgt
     1261 tcttgaacgc ggacgacccc cttttgatgg atcgcaaaaa gtccgcccgg tgttccgtgg
     1321 tgaccttcgg tcggtcgccc ggggccgacg tgcgggcgga aaacatccgc caggaacccc
     1381 gggtcgtttt cgatttggtc atggccggcg cccgccgtcc ggtgcaattg cccgtgcccg
     1441 gaattttcaa cgtcaccaac gccctggcgg ccgccgccgt cgcgctctgg gagcgggttc
     1501 cgctgccgtc cctcacgtcg gccttggagt cctttgcacc gccccccggt cgaatggaag
     1561 tgcgtcgtcg gaaggacggc acgttgttcg tcctcgacgc ctacaacgcc aatccggact
     1621 ccatggcggc ggggttgcgc agttttgtgg acgcatatcc cgagcgaccc cggtacgccg
     1681 ttttaggtag catgctggaa ttgggtcccc acgcggagcc cgagcaccgg gccctggggg
     1741 ccgggttggc cggtctgtcg ttcacggccg tgtattttgt gggagccgaa g
//
LOCUS       DAOQLS010000477         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47692_length_1791_cov_3.962558, whole genome shotgun sequence.
ACCESSION   DAOQLS010000477 DAOQLS010000000
VERSION     DAOQLS010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47692_length_1791_cov_3.962558"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1297
                     /locus_tag="PK362_08635"
     CDS             128..1297
                     /locus_tag="PK362_08635"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC74930.1"
                     /translation="MNPRRIYLASEATMKTLLLILLGLSSPWVLTGCRDASGPTATAK
                     LYRCPMHPTYTSHRPGECPICHMSLVAAEPDPAPAAEKRPLFYRNPMNPDVTSPVPAK
                     DEMGMDYVPVFAVESSSDAAVPGRGPVAVAADKEQWIGVRTAVVERRDLEVLVRASAR
                     VSYDPDLYAALAEHREALAARDRTGDPEGRARAESLVRASRLRLAQLGLTDKQMDVLS
                     QQGGGGLLAGAGPRWVYAQIFEDEAALIKPGQRAELTSPAFPGRVLNGRVEAVDRVVD
                     PATRTLRARVLVSDGESLLRPEMFLEAVVHVGLGRRLVAPKEALIDTGIRRLVYVKTA
                     PGHYAPREITTGREGENDVEVLTGLEAGETVAASGNFLLDSESRLKSVVSDGKAR"
     gene            1301..>1791
                     /locus_tag="PK362_08640"
     CDS             1301..>1791
                     /locus_tag="PK362_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC74931.1"
                     /translation="MIEKIIEWSARNKYLVLLLTGVAGLGAWVALHRVPLDAIPDLSD
                     TQVIVYSKWDRSPDIIEDQVTYPIVASLLGAPKIKAVRGFSDFGFSYVYIIFQDGTDL
                     YWARSRVLEYLSKIQPRLPEGVKTELGPDATGVGWVYQYALVDKSGEHDLAQLRGFQD
                     WTLR"
BASE COUNT          306 a          583 c          594 g          308 t
ORIGIN      
        1 acgccctgcg gtcccggttg gcggtggaaa ccgaatacca ggaagccctg gcccaggtcg
       61 ccaaccactg gggcatgttg gaagaatggg tcggcgcgcc gctttcgccg gcgggccggt
      121 gattcccatg aatcctcgtc gaatctatct cgcctcggag gcgaccatga aaactctgct
      181 tctaatcttg ctgggacttt cttcgccctg ggtgcttacc gggtgccggg acgcttcggg
      241 cccgaccgcc acggccaaat tgtaccggtg tcccatgcat ccgacgtata cgtcccatcg
      301 cccgggcgaa tgccccatct gccacatgtc cctggtggcg gccgagccgg acccggcgcc
      361 cgcggcggaa aagaggcctc ttttttaccg caatcccatg aatcccgacg tgacctcgcc
      421 cgtccccgcc aaagacgaga tgggcatgga ttacgtgccg gtgttcgccg tcgaatcctc
      481 ctccgacgcc gcggtgcccg gacgcgggcc cgtggccgtg gcggccgaca aggaacagtg
      541 gatcggcgtc cgcaccgcgg tcgtggagcg gcgggatttg gaagtgcttg tgcgcgccag
      601 cgcccgggtg tcctacgacc cggacctgta cgccgccctg gcggaacacc gggaggccct
      661 ggccgcgcgg gaccggaccg gcgatcccga aggacgcgcc cgggccgaat ccctggtccg
      721 cgcttcgcgc ctgcgcctgg cgcagctggg actgaccgac aagcaaatgg atgtcctgtc
      781 ccaacaaggc ggcggcggac tactggcggg cgcgggcccc cgttgggtgt acgcccagat
      841 ttttgaggac gaagcggcct tgatcaagcc cggccaacgg gccgagttga cgtcgcccgc
      901 cttccccggc cgggtgttga acggccgcgt ggaagccgtc gaccgggtgg tggaccccgc
      961 cacgcgcacc ctgcgggccc gggtcctggt gtccgacggg gaaagcttgc tgcggcccga
     1021 aatgtttttg gaggccgtgg tccacgtggg tttgggccgc cgcttggtgg ctcccaagga
     1081 agccctgatc gacacgggaa tccgccggct ggtgtatgtg aaaacggcgc ccgggcacta
     1141 cgccccccgg gagatcacga cgggccgcga aggggaaaac gacgtggagg tgttgaccgg
     1201 attggaagcc ggcgaaaccg tggcggcctc gggcaacttc ctgttggatt ccgaatcccg
     1261 gctcaaatcc gtcgttagcg acgggaaagc gcgttaagcc atgatcgaaa aaatcatcga
     1321 atggtcggcg cgcaacaaat acctggtcct actgttgacc ggggtggcgg gcctgggggc
     1381 ttgggtcgcc cttcaccggg tgcccttgga cgccattccg gacctttccg acacgcaggt
     1441 catcgtgtac tccaagtggg accggagccc ggacatcatc gaagaccagg taacctaccc
     1501 catcgtggcg tcgctcctgg gcgcgccgaa aatcaaagcc gtgcggggat tctcggactt
     1561 cgggttctcc tacgtctaca tcatcttcca ggacgggacg gacctctact gggcccgcag
     1621 ccgcgtgctg gaatatctgt ccaaaattca gccgcgcctg ccggaagggg ttaaaacgga
     1681 gctcggtccc gacgcgacgg gcgtcggatg ggtttaccaa tacgctctgg tcgataaatc
     1741 cggcgaacac gacctggccc aactccgggg gtttcaggac tggaccctcc g
//
LOCUS       DAOQLS010000478         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_47975_length_1786_cov_3.279607, whole genome shotgun sequence.
ACCESSION   DAOQLS010000478 DAOQLS010000000
VERSION     DAOQLS010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47975_length_1786_cov_3.279607"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1786
                     /gene="carB"
                     /locus_tag="PK362_08645"
     CDS             <1..>1786
                     /gene="carB"
                     /locus_tag="PK362_08645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNC74932.1"
                     /translation="DPMGVHTGDSITVAPAQTLSDPEYQVLRNQAFACIRAIGVETGG
                     SNVQFAVDPKTGRTVIIEMNPRVSRSSALASKATGFPIAKIAALLAVGYRLDEIPNDI
                     TKKTPACFEPVIDYVVTKVPRFAFEKFTEADQTLTTSMKSVGEVMAMGRTFRESLQKA
                     LRGLEIGRAGLGADGKSIVPKVDAAEKRLAAGEADPERAKLIEDIQHKLRVPNCDRIF
                     YIKYAFQLGLSVEDIHRLSAIDPWFLEQIQRIWILEKEVRAAGKKLPMELLYRAKRDG
                     FSDAQIAYLTGLSADAVAKTRKKNFPVAFKRVDTCAAEFPADTPYFYSTYETEDEFVP
                     LSKKDRVIVLGGGPNRIGQGIEFDYCCVHAAWAIKECGHEAVMVNCNPETVSTDYDTS
                     DQLFFEPLTLEDVVNVVERIKAKGVIPQFGGQTPLNLAKALLKAGVKILGTSADSIDI
                     AEDRERFGALLKKLNIPHPAHGTARNIKEAEAAAERVGYPVMVRPSYVLGGRAMEVVY
                     DKTQLADYMRRALESGAGLPILLDRFLEDAKEVDVDAICDGKNVYIAGIMEHIEEAGI
                     HSGDSACVLPPYSLEPKVIAALERHTKDM"
BASE COUNT          378 a          584 c          512 g          312 t
ORIGIN      
        1 gaccccatgg gcgttcacac cggggattcc atcaccgtcg ctccggcgca gaccctgtcg
       61 gatccggaat accaagtgtt gcggaaccag gctttcgcct gcatccgcgc catcggcgtc
      121 gaaaccggcg gttccaacgt ccaatttgcc gtcgatccca aaaccggtcg gacggtcatc
      181 atcgaaatga atccccgggt gtcccggtcc tccgcgttgg cctccaaggc gacgggattc
      241 cccatcgcca aaatcgcggc gctcctggcc gtgggctacc gactggacga gatccccaac
      301 gacatcacga aaaagacgcc ggcctgcttc gagcccgtga tcgattacgt ggtgacgaag
      361 gtgccccgtt ttgcctttga gaaattcacc gaggccgacc agacgctcac gaccagcatg
      421 aaatccgtgg gcgaagtcat ggccatgggc cggaccttcc gggaatccct tcaaaaggcc
      481 ctgcggggcc tggaaatcgg ccgcgcgggc ctcggggccg acggcaaatc cattgtgccc
      541 aaggtcgacg ccgccgaaaa acgattggcc gcgggcgaag ccgacccgga acgcgccaag
      601 ctgatcgaag acattcaaca caaattacgg gtccccaact gcgaccggat tttctacatc
      661 aaatacgctt tccaattggg cctatccgtc gaggacatcc accgcctttc ggccatcgat
      721 ccctggtttc tggagcaaat ccagcggatt tggatcctgg aaaaagaggt tcgggccgcg
      781 gggaaaaagc ttccgatgga attgctctac cgggccaagc gcgacggttt ttccgacgcc
      841 cagatcgcct atctcacggg cttgtccgcc gatgccgtgg ccaaaacgcg gaaaaagaat
      901 ttccccgtgg cctttaagcg cgtggacacc tgcgcggccg aatttccggc cgacaccccc
      961 tatttttatt cgacctacga aaccgaggac gagttcgtcc cgctgtccaa aaaggaccgg
     1021 gtgatcgtcc tgggcggcgg ccccaaccgc atcggccagg gcatcgaatt cgactactgc
     1081 tgcgtgcacg cggcctgggc catcaaggag tgcggccacg aagccgtcat ggtcaactgc
     1141 aacccggaaa ccgtctccac ggattacgac acctcggacc agttgttttt tgagcccctc
     1201 accctggaag acgtggtcaa cgtggtggag cgcatcaagg ccaaaggcgt cattccccaa
     1261 ttcgggggcc agacgccgct caacttggcg aaggccctgc tcaaggccgg cgtcaaaatt
     1321 ctgggaacct cggccgattc catcgacatc gccgaggacc gggaacgttt cggcgccctc
     1381 ctcaaaaaat tgaacattcc ccaccccgcc cacggcaccg cccggaacat caaagaagcc
     1441 gaggcggccg ccgagcgcgt gggctatccc gtcatggtcc gcccctccta cgtgttgggc
     1501 ggccgcgcga tggaagtggt ttacgacaaa acccaactgg ccgattacat gcgccgggcg
     1561 ttggagtccg gcgccgggtt gccgatcctg ctggaccggt tcctggaaga cgccaaggag
     1621 gtggacgtcg acgccatttg cgacggaaaa aacgtctaca tcgccggcat catggaacac
     1681 attgaagaag cgggcatcca ttccggcgac tccgcctgcg tgttgccgcc ctacagtttg
     1741 gagcccaagg tcatcgccgc cctggagcgc cacaccaagg acatgg
//
LOCUS       DAOQLS010000479         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48162_length_1783_cov_3.037037, whole genome shotgun sequence.
ACCESSION   DAOQLS010000479 DAOQLS010000000
VERSION     DAOQLS010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48162_length_1783_cov_3.037037"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PK362_08650"
     CDS             complement(<1..1535)
                     /locus_tag="PK362_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74933.1"
                     /translation="MRARGVLVFLFFLPALLGALEPPAVVPDAAPADAGRWAERPIRL
                     PPLFWWEREGDRRFLMASLIYWDWKDTAASQKLLFPLFYSGREPDRSLLVSLPFVAAY
                     RRGAERWMVAGPFYRSRGGVFARTAFFPVYWQQTRLGGGRATMFLPFLFYDYRNTDRS
                     KIDQAWPLGFRRVRGTESRGWILNYVWARAPDEGFQTLFPLYWHTHSPERRTDVVGPV
                     YRRWEARPDGDRRYAGLFPLAGAGGTVGSAAARSSYLFPVYFYQREPNHSLWLTVLAS
                     RERIADRRRGHLLLYYYDRDPDQSGAGVFPVFYHGASADGFSRVTQALNFYSRREGDD
                     RFQTLFPLYGLWTNTEGSRFLSWGIWRRRTEWLTPEGTPRGTSTSGWAFLYHWKRDSL
                     GNDTRVLFPLYWHYNRPPDWGMDLAFPLYFHYRDGPLSLTASPFFVHARREDKESWSW
                     LFLYWRNDFGPKKADLLFPFYYRERGPRRWTVVTPVFWSRRSALSREGALPLAYWYAS
                     REKA"
     gene            complement(1547..>1783)
                     /locus_tag="PK362_08655"
     CDS             complement(1547..>1783)
                     /locus_tag="PK362_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009787248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNC74934.1"
                     /translation="EMLRQLTSLKDRIPGIIGVSAGVNFSARAQGYTHGFVVRFRDRA
                     ALEAYLPHPAHQAVVEHHVRPISEGVLALDYEFQ"
BASE COUNT          390 a          553 c          588 g          252 t
ORIGIN      
        1 gccttttccc ggctggcgta ccaataggcc aggggaaggg cgccttcgcg ggacaaggcg
       61 ctccgccggg accaaaagac cggcgtgacc acggtccacc ggcggggccc ccgctcgcgg
      121 taataaaaag ggaaaagcag gtcggctttc ttggggccga aatcgtttcg ccagtaaagg
      181 aacaaccaac tccaactttc cttgtcctcc cggcgggcat ggacgaaaaa gggcgaggcg
      241 gtaagggaaa ggggcccgtc ccggtagtgg aagtagaggg ggaacgccaa atccatcccc
      301 caatccgggg gacgattgta gtgccaatac aacgggaaca acacccgggt gtcgttgccg
      361 agcgagtctc ttttccaatg atagaggaac gcccaaccgc tggtggacgt cccccggggg
      421 gttccttccg gggtcaacca ttccgtccgg cggcgccaaa tcccccagga caaaaaccgg
      481 gacccctcgg tatttgtcca aaggccgtag agggggaaga gggtctgaaa ccggtcgtcc
      541 ccctcccggc ggctgtaaaa attgagcgcc tgggtgaccc gggaaaaacc gtcggcgctg
      601 gcgccatgat aaaaaaccgg aaagaccccg gcgccgcttt gatcggggtc ccgatcgtaa
      661 taatagagca acaggtgccc ccgtcggcgg tcggcgatcc gttcccggct ggccagaacc
      721 gtcagccaca gggaatggtt cggctcccgt tgataaaagt aaacgggaaa tagataggaa
      781 ctgcgcgcgg cggccgagcc caccgtcccc cccgcccccg ccagggggaa gaggcccgcg
      841 taccggcgat ccccgtcggg acgggcttcc catcggcggt atacgggccc caccacgtcg
      901 gtccgtcgtt cgggggaatg ggtgtgccaa tagagcggga acagggtttg gaagccctcg
      961 tccggcgccc gggcccagac gtagtttaaa atccagcctc gggattccgt gccccgcacc
     1021 cggcgaaacc ccaggggcca ggcctggtcg attttggaac ggtcggtgtt ccggtaatcg
     1081 taaaagagga aaggcaaaaa catggtggcg cgtccgccgc ccaaccgggt ttgttgccaa
     1141 tagaccggga agaacgccgt tcgggcgaag accccgcccc gggaacggta aaacggtccg
     1201 gccaccatcc accgctccgc gccccgtcga taggcggcca caaaaggcag cgacaccaga
     1261 agggaccggt cgggttcccg gccgctgtaa aacaacggga aaagcagttt ttgggaggcg
     1321 gccgtgtcct tccaatccca atagatcagg gacgccatca aaaaccggcg gtcgccctcc
     1381 cgttcccacc aaaagagggg cggcaaacga atgggacgct ccgcccagcg gccggcgtcg
     1441 gcgggggcgg catcgggaac aacggcgggg ggttcaaggg cgcccagaag ggcgggaagg
     1501 aaaaaaagga atacgagaac cccgcgggcg cgcaccggcg ggggccttac tgaaattcgt
     1561 agtcgagggc cagcacgccc tcgctgatgg gacgtacgtg gtgttccacc accgcctgat
     1621 gggcggggtg cggcaaatag gcctccaagg cggcgcgatc gcgaaaccgc accacgaaac
     1681 cgtgggtgta cccctgggcc cgggcgctga aattgacgcc ggccgacacc ccgataatgc
     1741 ccgggatccg gtccttgagc gacgtcaact gacggagcat ttc
//
LOCUS       DAOQLS010000480         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48295_length_1780_cov_4.711884, whole genome shotgun sequence.
ACCESSION   DAOQLS010000480 DAOQLS010000000
VERSION     DAOQLS010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48295_length_1780_cov_4.711884"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PK362_08660"
     CDS             <1..661
                     /locus_tag="PK362_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HNC74935.1"
                     /translation="YASGRTGWYTSSVSAKRMSRRGDSLSLGVDRVTRFTQSDDAVVA
                     DTYVSVWPRAYTNLRLQSARRVRILPKNDGFFELYQGFGHQWEASARVRHMSFVGDNV
                     DLYSLSLAKYYNEWYGQSRVTWVPSRDGVGVSREFVIRRYGGNEDEFVQLTLGRTSHS
                     AVGAFTPGATGDQSTYAHARLQVLARDWIGLSGTLMLEDEIHLPPRKGFSLQVFLRYN
                     D"
     gene            700..1332
                     /locus_tag="PK362_08665"
     CDS             700..1332
                     /locus_tag="PK362_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC74936.1"
                     /translation="MENFCYLVGDPAVKKCWIVDPAWDVKTALDIAAADGYTVDGALI
                     SHSHFDHCNAVESLLADRNIPLYVQPRELEYLEKGAPRGLFWDLPKDHVRPVKGGDVL
                     SLGATNLTVLHTPGHTPGSQCFLVDGRLFSGDTLFLGTCGRCDLPGGNPHELFETLNG
                     VLAKLPDDTVLFPGHNYSSRGTQSTLREEKKENRFFSVRRLDDFLEKLGF"
     gene            1335..>1780
                     /locus_tag="PK362_08670"
     CDS             1335..>1780
                     /locus_tag="PK362_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74937.1"
                     /translation="MAAPFSIFDHLALGAGAGMAPLSVFHLTGTSIVPFANDLFSSDV
                     SALAPGRGTLTCLLTAQGKLVSRVFIHHRGHEMLFLTPTDGAAGFRDAMAKYAGGSEH
                     RFEQMTGWVAVFLTGAQADVRVNSLPKAFHVDYRLLGVDGYIGVVP"
BASE COUNT          317 a          551 c          525 g          387 t
ORIGIN      
        1 gtacgcctcg ggccgtacgg gttggtacac gtcctccgtc tcggccaaac ggatgtcgag
       61 gcgaggcgat tctctttccc tgggcgtgga ccgggtcacg cggttcaccc aaagcgacga
      121 cgccgtcgtc gcggacacct atgtcagcgt gtggccccgg gcctacacca acctccgact
      181 ccaatccgcg cgccgcgtcc ggatcctgcc gaagaacgac gggtttttcg aactctacca
      241 ggggtttggc catcagtggg aggcatcggc ccgggtccgc cacatgagtt ttgtggggga
      301 caacgtcgac ctttattcgc tctctttggc gaagtattac aacgaatggt acggacaatc
      361 ccgggtcacc tgggttccgt cccgggacgg ggtcggggtt tcccgggaat tcgtcattcg
      421 acgatacggc ggcaacgagg acgaatttgt tcaattgact ctcggccgca ccagccattc
      481 ggccgtgggc gcattcacgc cgggggccac gggcgatcaa tcgacctacg cccacgcccg
      541 cctgcaggtt ctggcccggg attggatcgg tctctcgggc acgctgatgt tggaggacga
      601 aatccacctg ccgccaagaa aaggcttttc gctgcaggtc tttctgcgat acaatgactg
      661 attatgcccc cgtacctgcg ccaaattccg gtgggtccca tggaaaactt ttgctatttg
      721 gtgggggacc ccgcggtcaa aaaatgttgg atcgtcgacc ccgcctggga cgttaaaacc
      781 gccttggaca tcgccgccgc cgacggctac acggtcgacg gcgccctcat cagccacagc
      841 catttcgatc attgcaacgc cgtcgagtct cttttggccg accgcaacat ccccttgtat
      901 gttcaacccc gcgaactgga atacctggaa aagggcgctc cccggggatt gttttgggat
      961 ttgccgaagg accacgtgcg gcccgtgaaa gggggcgacg tcttgtcgtt gggcgcgacg
     1021 aacctgacgg tccttcacac ccccggccac accccgggtt cccaatgctt cctggtcgac
     1081 ggccgtcttt tcagcggcga cacgcttttt cttgggacct gcggccgctg cgatcttccg
     1141 ggtggaaatc cacacgaact gttcgaaacc ctgaacgggg tgttggccaa acttccggac
     1201 gacaccgttt tgttccccgg acacaattat tcctcacggg gaacccagag caccctgcgg
     1261 gaagaaaaaa aagaaaatcg ttttttcagt gttcgacggc tcgacgactt cctcgaaaag
     1321 ctgggatttt agtcgtggcc gcccccttct ccatctttga tcatctggcc ctcggcgccg
     1381 gcgccggcat ggcgccgttg agcgtttttc atttaaccgg aacgtcgatc gttcccttcg
     1441 ccaacgacct tttttcaagc gacgtttccg ccctggctcc cgggcgggga accctcacgt
     1501 gtttgctgac cgcccagggg aaattggtgt cccgggtgtt tattcaccac cgaggccacg
     1561 aaatgctttt tttgacgccg acggacggcg cggcggggtt tcgcgacgcc atggcgaaat
     1621 acgcgggggg gtcggagcac cgctttgaac agatgaccgg ttgggtggcg gttttcttga
     1681 cgggcgctca ggccgacgtc cgcgtgaatt ccctgccaaa ggcctttcac gtggattacc
     1741 gcctcctggg ggtcgatggg tatatcggcg tcgtccccat
//
LOCUS       DAOQLS010000481         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48449_length_1777_cov_3.565041, whole genome shotgun sequence.
ACCESSION   DAOQLS010000481 DAOQLS010000000
VERSION     DAOQLS010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48449_length_1777_cov_3.565041"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..997
                     /locus_tag="PK362_08675"
     CDS             44..997
                     /locus_tag="PK362_08675"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HNC74938.1"
                     /translation="MAFLNRIQTSWRLMKSSLSVMVDHPKLLVFPAVITACTFGILLF
                     FLAPVALQPTGHGYAEGAHWKAVSESIFTRESMDQMAAAKPEERRNQQLALTPQAMVY
                     LAVIYFVSMFLATFFTTAFYHEILSALQGGGVSVTGGLRFALTKLPAILMWSLFAGAI
                     GYLIKSLEERVGLIGKWILRFIGVAWSVASVFAIPVLVMEEEKNPVAILKRSAETLKR
                     TWGESLAGFVGLQFGGFIGILATIGLFTVGIFAASTFKMPLLLLATTGLWIAGLFAFF
                     YTMGVANSIYRCALYVFAARGTLAGPYDREAMDLAWKMKKK"
     gene            complement(1002..1403)
                     /locus_tag="PK362_08680"
     CDS             complement(1002..1403)
                     /locus_tag="PK362_08680"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNC74939.1"
                     /translation="MFKVVREIHFCYGHRLMNYAGKCRHPHGHNGRVEVEVSARKLDP
                     RGMVVDFGDIKSRLQSWIDDHLDHGMILRKDDPLVKTFKNLGEPVYLMADNPTAENIA
                     KEIFTVARKRGLPVTRVTHWETEKSWASYQP"
     gene            1478..1726
                     /locus_tag="PK362_08685"
     CDS             1478..1726
                     /locus_tag="PK362_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74940.1"
                     /translation="MSRGEKIVASVLGILVFFGLFFFIYSSMKTTPAYPPVDEAAADY
                     GDDYNFNVEGDTTTVDEALPEEPAPAETTKKDEKQPKK"
BASE COUNT          351 a          500 c          507 g          419 t
ORIGIN      
        1 ataggagatc ggtgtctcac gaacccctga cggaggattc cccatggcct ttctgaaccg
       61 catccaaacc agttggcgtt tgatgaaaag ctctctgtcc gtcatggtcg atcatccgaa
      121 gcttttggtt tttccggcgg tgatcacggc ctgcaccttt gggatcctcc tattcttttt
      181 agcccccgtg gcccttcaac ccacgggcca cggctacgcc gaaggggccc attggaaagc
      241 cgtcagcgag agtattttca cccgggagtc catggatcaa atggccgccg cgaaacccga
      301 ggaacggcga aatcaacaat tggccttgac cccccaggcc atggtgtatt tggcggtgat
      361 ctatttcgtc tccatgtttt tggcgacctt tttcaccacc gccttctacc acgagatttt
      421 gagcgccctc cagggcgggg gtgtctcggt gacgggcgga ctgcgtttcg ctttgaccaa
      481 actcccggcg attctgatgt ggtccctctt cgccggcgcg atcggctatc tcattaaatc
      541 cctggaagag cgtgtggggt tgatcggaaa atggatcctt cgattcatcg gcgtggcctg
      601 gagcgtggcc tcggtgttcg ccattccggt cctcgtgatg gaggaggaaa aaaacccggt
      661 ggcgatccta aaacgctccg cggagaccct taagagaacc tggggggaat ccctggccgg
      721 gttcgtgggg cttcagttcg gcgggttcat cgggattctt gcaaccatcg gccttttcac
      781 ggtgggaatc ttcgcggcgt ccacctttaa aatgccgctg cttcttttgg cgacgacggg
      841 gttgtggatc gcggggctct ttgcgttttt ctacacgatg ggcgtggcca acagcatcta
      901 ccgttgcgcg ctctatgtgt tcgccgcccg gggcaccctg gcgggtccct acgaccggga
      961 ggccatggac ctggcgtgga aaatgaaaaa gaaatgacgg gtcagggctg gtaagaggcc
     1021 caggactttt cggtttccca gtgggtcacg cgggtcacgg gcaacccccg cttccgggcc
     1081 acggtgaaaa tttccttggc aatgttttcg gcggtggggt tgtccgccat caggtaaacc
     1141 ggttcgccca gatttttgaa cgttttgacc aggggatcgt cttttcgaag aatcatcccg
     1201 tggtccaggt gatcgtcgat ccagctttgc aggcggcttt tgatgtcccc gaaatccacc
     1261 accatccccc gcgggtccag ttttcgggcc gaaacctcca cttccacccg gccgttgtgg
     1321 ccgtggggat ggcggcactt gcccgcgtaa ttcatcaggc ggtggccata gcagaagtga
     1381 atttcccgga cgactttgaa cacggggatt atggtaaaat actctatccg gtcggtcaac
     1441 aaatcgattt gaaatcaagg gttaaaagga gattcgcatg agtcgggggg agaaaattgt
     1501 cgcgtccgtt ctggggattt tggtgttttt cggcctcttt ttcttcattt attcttccat
     1561 gaaaaccacc cccgcctacc ccccggtgga cgaagccgcg gccgattacg gcgatgatta
     1621 caatttcaac gtggaaggcg acacgaccac ggtggatgaa gcccttccgg aagaacccgc
     1681 ccccgcggaa acaaccaaaa aggatgaaaa acagcctaag aaatagggct gttttgcgat
     1741 tcccttgcac caaccccgac gcaacgccag ccacctt
//
LOCUS       DAOQLS010000482         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48541_length_1775_cov_4.389535, whole genome shotgun sequence.
ACCESSION   DAOQLS010000482 DAOQLS010000000
VERSION     DAOQLS010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48541_length_1775_cov_4.389535"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1775)
                     /locus_tag="PK362_08690"
     CDS             complement(<1..>1775)
                     /locus_tag="PK362_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74941.1"
                     /translation="LWPAVLSRESIEHSRADVLSTTVLDDWIALMRPGDVVFLRFPFY
                     FLLEWNRREGGRLEAWFENFIRKIPAGVPVVLIEGGFRTSTWMERAHRIFARNGFGLS
                     MENRLLPAERSALGRANAVLEKTGPLEMFVGLHLNLALGGALWVYEKLPQPLAAVPGW
                     VRGRSTPGNLLGLFAVVAAAGALFASWLSPEYTALARGVSDALAALGGSPEPALWAVG
                     MGPVFGLGVVRDRGSREERTAGPSPEDDRPVRAGLLDAAHVAYDEAVRRALDPEGRRP
                     VGLYLGAGADLSNFFLSTNVDRAFFLAQNYRSAGRGPRLTLEAFRQALKNPAGFVLKF
                     PRLWAGVRFKKDYSNFKRVWGYAFGPHVSTPGRFLAALVLELKALGVDPRSATPFEST
                     EGNLGLRFLWSHPGEKPRERTVVFIDGTAASPVDLERTLQTLPPLDVVYQRAGELIPL
                     LIEADPRLVAVMNRRMAPGGRFAINPYARNWGSRQTIDRKIVFPFPLLEVSIPEEESR
                     AARVTERQLRAAGALDLLDDTYPRDNPYGFRMAIREAAPEVDLLAEERAPSAVLDWFD
                     GDVAMDGASSESVEALARESVRWAR"
BASE COUNT          347 a          606 c          577 g          245 t
ORIGIN      
        1 ccgggcccag cgaacgcttt cccgggcgag ggcctcgacg gattcggagg acgccccgtc
       61 catggccacg tcgccgtcga accaatccaa aaccgccgag ggagcgcgct cctccgccag
      121 caaatcgact tccggcgcgg cttcgcggat ggccattcga aaaccgtagg gattgtcccg
      181 gggataggtg tcgtccaaca ggtccagggc gccggccgcc cgaagctggc gttcggtcac
      241 ccgggcggcc cgcgactctt cctccggaat cgacacctcc agcaggggaa acgggaaaac
      301 gattttccgg tcgatggtct ggcggctccc ccaattgcgg gcgtagggat tgatcgcgaa
      361 ccggccgccg ggcgccattc gtcggttcat taccgcgacc aaccggggat ccgcctcgat
      421 caacagcggg atcagttcgc ccgcccgttg gtagacgacg tccaaggggg ggagggtttg
      481 aagggtgcgc tccaaatcca cgggcgaagc ggcggtgccg tcgatgaaaa caaccgtccg
      541 ctcccggggc ttttcgcccg gatggctcca gaggaagcgg aggcccagat tcccttcggt
      601 cgattcaaag ggcgtggccg agcggggatc gacgcccagc gctttgagct ccaggaccag
      661 cgcggccaaa aaccgtccgg gggtggaaac gtggggaccg aaggcgtacc cccaaacccg
      721 cttgaaattc gaatagtctt ttttgaaccg gacgccggcc cacaagcgcg ggaacttcaa
      781 aacgaaaccc gcgggatttt tgagggcctg gcggaaggcc tcgagggtca accgcgggcc
      841 ccgccccgcg ctccgatagt tttgggcgag gaagaacgcc cggtcaacgt tggtggaaag
      901 gaaaaaattg ctcaggtccg ccccggcccc cagatacagc cccaccggtc gccgaccctc
      961 cggatccagg gcccgtcgaa ccgcctcgtc gtaggccacg tgggcggcgt ccaacaggcc
     1021 ggcgcgaacg ggccggtcgt cctcggggga cggtcccgcg gttcgttcct cccgggatcc
     1081 gcggtcccgc accacgccga gaccgaacac ggggcccatt cccaccgccc acagggccgg
     1141 ttcgggcgaa cccccgaggg ccgccagggc gtccgaaacc ccccgagcca gggcggtgta
     1201 ctccggcgaa agccaagacg cgaacagcgc cccggcggcg gccacgacgg cgaagaggcc
     1261 caaaaggttt ccaggcgtcg aacgcccccg gacccacccc ggcacggcgg ccagcggctg
     1321 cggaagtttt tcgtagaccc acagggcccc gcccagggcc aggttcaaat gcaggccgac
     1381 gaacatctcc agggggccgg tcttctccag gacggcgttg gcccggccga gggcggaccg
     1441 ttccgcgggg agaagtcgat tctccatcga aaggccgaac ccgttccggg cgaaaatccg
     1501 atgggcgcgc tccatccagg tggacgttcg aaatccgcct tcgatcagca ccaccggaac
     1561 cccggcggga atctttcgga tgaaattctc gaaccaggcc tccagacggc ccccctcccg
     1621 gcggttccat tccaaaagga aatagaaagg aaaacggaga aagaccacgt cgcccgggcg
     1681 catgagcgcg atccaatcat caagaaccgt cgtgcttagc acgtcggccc ggctgtgttc
     1741 gatggattcc cgcgacaaca ccgcgggcca caggg
//
LOCUS       DAOQLS010000483         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48643_length_1773_cov_4.072759, whole genome shotgun sequence.
ACCESSION   DAOQLS010000483 DAOQLS010000000
VERSION     DAOQLS010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48643_length_1773_cov_4.072759"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PK362_08695"
     CDS             <1..346
                     /locus_tag="PK362_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNC74942.1"
                     /translation="DAVVVGADRIAANGDTANKIGTYALAVLARHHRVPFYVAAPFST
                     VDAKTATGRDIPIEERSVAEVTSFRGMSIAPRGARARHPAFDVTPAALVTAFVTDRGV
                     LRRIPALPRGAR"
     gene            333..>1773
                     /locus_tag="PK362_08700"
     CDS             333..>1773
                     /locus_tag="PK362_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC74943.1"
                     /translation="MAPVNPAPRRAVPGWAFPAAVALFVLWTYWPALSGAFLDWDDNH
                     LLLLNPHFRGLGREAWRWAWTNNPGLYMPLVWMSFSLDSALWGMNPVGFHLTNVLFHA
                     ANAVLLFFVAETLFERLFPRSSGGARRGAAALASLLWAVHPLRVESVAWIVERKDVLC
                     GFFFLLSLLFYLRFAATGRPGERWLSAGAHLAGMLSKPVAVLGPLVFLLLDWRPLARW
                     PGRPWKVLLREKIPFFLASGAAALLTIAPGGRPSDVGPAERLGQALFGLGFYLQKTLW
                     PTGLAPVYEWRGEILRWSGPPVFFMAIGAGCLLLWLFSRRRWPSFWIAGASYLLLLAP
                     LLGLIPRGPQSAADRFTYLPGLGLALLAAALPLFLSVRNRRAGAVAAFLLAAALGPLT
                     AATRRQIGLWKNSETLWRWQLERFPSSFLGHYNLGTYLLNQDRPSEALPHLDAAVRLH
                     PDVPDPFNNRGRAFFALDRLEEAGRDFDQA"
BASE COUNT          202 a          646 c          555 g          370 t
ORIGIN      
        1 ggacgccgtc gtggtgggcg ccgaccgcat cgccgccaac ggcgataccg ccaacaagat
       61 cggcacctac gccctggccg ttttggcccg tcaccaccgg gttccctttt acgtggccgc
      121 ccccttttcc acggtggacg cgaaaaccgc gacggggcgg gacatcccca tcgaagaacg
      181 gtccgtggcc gaagtgactt cctttcgggg aatgtccatc gccccccggg gggcccgggc
      241 ccgccatccc gcctttgacg tcacgcccgc ggccctggtg acggccttcg tcaccgaccg
      301 cggcgtcctt cgccgaattc ccgcccttcc gcgtggcgcc cgttaacccg gccccgcgcc
      361 gcgccgttcc ggggtgggcg tttcccgcgg cggtcgccct tttcgtcctg tggacctact
      421 ggccggcgtt gtccggcgcc ttcctcgact gggacgacaa ccacctgctt ttgctcaacc
      481 cccatttccg gggactcggc cgcgaggcct ggcgttgggc ctggaccaac aaccccggcc
      541 tttacatgcc gctcgtgtgg atgagctttt cgctggacag cgccctgtgg ggaatgaacc
      601 ccgtggggtt ccatctcacc aatgtgttgt ttcacgcggc caacgccgtc ctcttgtttt
      661 tcgtggcgga aacgcttttc gagagactct ttccccggtc ctccggcggc gcccgccggg
      721 gggcggccgc cctggcgtcg cttctgtggg cggtccatcc gctccgggtg gaatccgtcg
      781 cctggatcgt cgaacggaaa gacgtgctgt gcggtttctt cttccttctc tccctgcttt
      841 tctatcttcg gttcgcggcg acgggacggc ccggggagcg ctggctctcg gcgggggccc
      901 acctggccgg catgctttcc aaacccgtgg cggttctggg gccgctggtg ttccttctcc
      961 tggattggcg gcccctggcc cgctggccgg ggcgaccctg gaaagttctc cttcgggaaa
     1021 agattccgtt ctttctcgct tcgggcgccg ccgccctgct caccatcgct cccggcggtc
     1081 gaccctcgga cgtgggaccg gcggagcgcc tcggccaggc cctttttggc ctcgggtttt
     1141 atctgcagaa aaccctctgg ccgacgggcc tcgcaccggt gtatgaatgg cggggcgaaa
     1201 tcctccgatg gtccgggccc ccggtgttct tcatggcgat cggggcgggg tgtctgcttc
     1261 tctggttatt ttcccgacgg cggtggccgt cgttctggat cgcgggggcg tcgtatctcc
     1321 tgctcctggc cccccttctg ggtttgatcc cccggggccc ccaatcggcg gccgatcgat
     1381 tcacgtacct gccgggcctg ggcctggccc tcctggccgc ggcccttccc ctgtttctct
     1441 cggtcagaaa ccgccgggcg ggcgccgtcg ccgccttcct cctggccgcc gcgctggggc
     1501 ccctgaccgc cgccacccgt cgacaaatcg gcctttggaa aaattccgag acgctctggc
     1561 ggtggcagtt ggagcggttc ccctcctcgt ttctggggca ttacaacctg gggacctatc
     1621 tgctcaatca agaccgccca tcggaggccc tgccccatct ggacgcggcg gttcgccttc
     1681 atcccgacgt gcccgacccc ttcaacaacc ggggacgcgc ctttttcgcc ctggaccgac
     1741 tggaggaggc gggccgggac ttcgatcaag ccc
//
LOCUS       DAOQLS010000484         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48666_length_1773_cov_2.821304, whole genome shotgun sequence.
ACCESSION   DAOQLS010000484 DAOQLS010000000
VERSION     DAOQLS010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48666_length_1773_cov_2.821304"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="PK362_08705"
     CDS             <1..1566
                     /locus_tag="PK362_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74944.1"
                     /translation="PVVSPLLRGRLSKPLFQSWLNGQTARAASSREGPAAWRLLQTLD
                     PGFSDDGAPPTASEGLGAVAAKGFRGASAAVARLFTHPATPALLTLVTLPVLFGANLL
                     GLLAAFQMVGWNAPSFAAVLSFIVAVAGAMAEHKLLSDLRIEFWEKSRAPASVREAPP
                     ATTVRDVTLEVANDRAASVEALMGLVLNARRGGDVDAERELARLVAAQVALDPMRNDL
                     LDGFDALKTRRWMREALRERANEGRPGRRPAADFLLNEWLRVPWGQQLAQIGRARASG
                     LRPEAELRQSAYLAGLSGAERSMAKHVLTVAARSAEMELPSDWAQAWEHGHALGNHWW
                     GAPGVPATEASPALITALRVTELLNGAGGSPSTREAVTAQLKERAGRDARRPDILIVN
                     GSWMSEDRRGWTEERVKRELRKELRDRRNGQLDPVLAAYLKKSRVLVIAADAFSPDAV
                     LRFDAGPAHRRGKTLNLLTADLNAIVFDPRREDAPFRVLLLQCLSGRLTVLDVGEWAR
                     RALKTARVVVTSA"
BASE COUNT          269 a          581 c          601 g          322 t
ORIGIN      
        1 cccgtcgtgt cgccccttct tcgcgggagg ctgtcgaaac cgctctttca atcctggtta
       61 aacggtcaaa cggcgcgcgc cgcctcgtcc cgggaaggcc cggcggcctg gcgcctcctc
      121 caaacgttgg accccggttt ttccgatgat ggagctcccc cgacggcttc cgagggtctc
      181 ggggccgtgg cggcgaaagg gtttcgcggc gcctcggcgg ccgtggcccg tcttttcacg
      241 cacccggcga cgccggccct tttgaccctc gtgacgttgc cggttctctt cggggcgaat
      301 ctccttggtc tcctggccgc ttttcaaatg gtcggatgga acgcgccgtc ctttgcggcg
      361 gtgctgagtt tcatcgtcgc cgtcgccggc gcgatggccg aacataaatt gttgtcggac
      421 cttcggattg aattttggga aaaaagccgt gccccggcct cggtccggga agccccgccg
      481 gcgacaaccg tccgcgacgt cacgttggaa gtggccaacg atcgcgccgc ctcggtggag
      541 gcgttgatgg gactggtcct taacgcccgc cggggcggcg atgtcgacgc cgaacgcgaa
      601 ttggcccgcc tggtcgcggc gcaggtggcg ttggacccga tgcggaacga tttgttggac
      661 gggtttgacg cgcttaaaac ccggcgctgg atgcgggagg ccctgcggga acgcgccaac
      721 gaggggcgcc ccggccggcg gcccgcggcg gattttctcc tgaacgaatg gcttcgggtg
      781 ccttgggggc aacaattggc gcaaatcggg cgcgcccggg cgtcgggtct ccgccccgag
      841 gcggaacttc gacaatccgc ctatctggcg gggttgtccg gcgctgaacg ttcgatggcg
      901 aagcacgttt tgacggtcgc ggcccggtcc gcggaaatgg aacttccgtc cgattgggcc
      961 caggcttggg agcacggcca cgccctcggg aaccactggt ggggcgcccc cggcgtgccc
     1021 gccacggagg cctcgcccgc cttgatcacg gccctccggg tcactgaact tctaaacggc
     1081 gcgggagggt cgccgagcac gcgggaagcg gtgacggctc aattgaagga gcgcgccggt
     1141 cgcgacgccc gtcgccccga tatcctgatc gtcaacgggt cttggatgtc cgaagaccgc
     1201 cgggggtgga cggaagaacg ggtcaaacgc gagttgcgaa aagaattgcg ggaccgtcgg
     1261 aacgggcaat tggacccggt ccttgccgct tacctcaaga aatcccgcgt cctcgtgatc
     1321 gcggcggacg cgttttcgcc cgacgcggtc ctgcggttcg acgccggccc ggcccaccgc
     1381 cggggaaaaa cgctcaattt gttgacggcg gatttgaacg ccatcgtgtt cgatccccgg
     1441 cgggaggacg cgccgttccg ggtattgctg ctccaatgtc tttccgggcg attgaccgtg
     1501 ctcgatgtgg gcgaatgggc gcgacgggct cttaaaacgg cgcgcgtcgt cgtcacgagc
     1561 gcctgattca cgtttccgga tccgaaaggt tataattccc ccggggcccg tcgaaacggc
     1621 ggggccgacc ttcccatgcg catcgccctg atcgatttag gaaccaacgc cgttcggttc
     1681 gatgtccacg acatcggccc ggacggcctc gcccgacgcc ttcaccggga acgcctcgcc
     1741 tccgaagtgg cagcccgcac cgccgagctg cag
//
LOCUS       DAOQLS010000485         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48758_length_1771_cov_4.113054, whole genome shotgun sequence.
ACCESSION   DAOQLS010000485 DAOQLS010000000
VERSION     DAOQLS010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48758_length_1771_cov_4.113054"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="PK362_08710"
     CDS             complement(<1..1198)
                     /locus_tag="PK362_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNC74945.1"
                     /translation="MMRNSWALALVLAGAAGLTAEACDLWGPLAAGDFKGVKSCVDSS
                     NVNVLSGSDSPLTAAAKFAFRDPADIVALLLDEGAKINFQGDERRTALMNAAGKAWTQ
                     SVDLLIKKGADVRPRDANGRTALMYAGDAYDPTIVDQLVAAGAKVNDADVDGRTPLMA
                     AVVRVRKMGTAAENDRMKTIERLLAAQADINARDKEGKTALMLAAESGQTEALRKLLA
                     AKAAVNLQSKDGRTALHHALFADKPVESKILMDAGADINLPDAEGRTPLMSVLDWRRD
                     ALVDDLLNRRADVNRKSNDGKTALMEAVEARREDYIQKLMQRKALINLQNKEGDTALM
                     IAARMNSGEKCVKTLLRYSPNLTLKNVAGKTALAIAQESRHEESVLFIRAQRDNGASG
                     TKSAMRL"
     gene            complement(1249..1617)
                     /locus_tag="PK362_08715"
     CDS             complement(1249..1617)
                     /locus_tag="PK362_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HNC74946.1"
                     /translation="MLRGLMGLIIGSVAGGLGRYAVTAGFARTSLRGFWATWTVNLAG
                     CFLMGLFHAWAEVRFRISVEQRLLLMSGFCGAFTTFSAFALDTAGLIRGGHGIAAAAY
                     VASSVVLGLGMFYAGAWVGR"
     gene            complement(1619..>1771)
                     /locus_tag="PK362_08720"
     CDS             complement(1619..>1771)
                     /locus_tag="PK362_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNC74947.1"
                     /translation="GGGYYDRFLARSSVQTAALAFPEQMATRLPRRPHDRRVGVVVTA
                     NRTYTP"
BASE COUNT          323 a          593 c          526 g          329 t
ORIGIN      
        1 ccaagcgcat cgccgacttc gtccccgagg cgccgttgtc ccgttgggcg cgaatgaata
       61 aaaccgattc ttcgtggcgg gattcctggg cgatggccaa cgccgttttg cccgcgacgt
      121 ttttaagcgt caggttgggc gagtagcgga gcagcgtttt gacgcatttc tcgccggaat
      181 tcatccgggc ggcgatcatc aacgccgtat cgccttcctt gttctgaagg ttgatcagcg
      241 ctttgcgctg catcaatttt tggatgtagt cttcccgacg cgcctccacc gcctccatca
      301 acgcggtctt cccgtcgttg gatttgcggt tcacgtcggc ccggcggttc aacaagtcgt
      361 cgaccagggc gtcccggcgc caatccaaga ccgacatcag cggcgtgcga ccttccgcgt
      421 cgggcaaatt aatgtcggcc cccgcgtcca tcaaaatttt gctttcgacg ggtttgtcgg
      481 cgaaaagggc gtggtgcaac gccgtccgcc cgtctttcga ttggaggttg acggcggctt
      541 tggccgcgag caacttgcgc agggcctcgg tttggccgct ctcggcggcc agcatgaggg
      601 ccgttttgcc ttccttgtcc cgggcgttga tgtcggcttg ggcggccaaa agccgctcga
      661 tggttttcat tcggtcgttt tcggcggcgg tgcccatttt gcgcacgcga accaccgcgg
      721 ccatgagggg cgtccgaccg tccacgtcgg cgtcgttgac cttggccccg gcggccacca
      781 attgatcgac gatggtcggg tcgtaggcgt ccccggcgta catcagggcc gttcgaccgt
      841 tggcgtcccg gggccgcaca tcggcccctt ttttaatcaa cagatccacc gattgggtcc
      901 aggccttccc cgcggcgttc atcaacgccg ttcgtctttc atcgccttga aaattgatct
      961 tcgcgccttc gtccaacaac agggccacga tgtccgcggg gtcccggaaa gcgaatttgg
     1021 ccgccgcggt caatggggaa tcggacccgg aaagcacgtt cacgttcgaa gaatccacgc
     1081 aggatttgac gcccttgaaa tcgccggccg cgaggggccc ccacaggtcg caggcttccg
     1141 cggtcaaacc ggccgcgccc gccaacacca gggccaaggc ccatgaattc cgcatcattg
     1201 gtgaaatcct cccattgaaa tcgtccacgg gcccggcggc gcccgtcttt aacggcccac
     1261 ccaggcgccg gcgtaaaaca ttccaagccc caaaaccacg cttgaggcga cgtaggccgc
     1321 cgcggcgatc ccgtggccgc cccgaatcaa gcccgcggtg tccagggcga aggcggagaa
     1381 ggtcgtgaac gccccgcaaa agcccgacat caacaacaga cgttgctcga cggaaatccg
     1441 aaagcggacc tcggcccagg cgtgaaacaa acccatcaaa aaacatcccg ccagattgac
     1501 ggtccaggtc gcccaaaagc cccggaggga ggtccgggcg aaccccgccg tcacggcgta
     1561 gcgccccaag cctccggcca cgctgccgat gatgagcccc atcaagccgc ggagcatctc
     1621 aaggagtata cgtccgatta gccgtgacga ccacgcccac ccggcgatcg tggggccgcc
     1681 gcggcaagcg cgtcgccatt tgttccggga aggccagcgc cgccgtttga accgacgaac
     1741 gcgccagaaa tcgatcgtag tatcccccgc c
//
LOCUS       DAOQLS010000486         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48761_length_1771_cov_3.993590, whole genome shotgun sequence.
ACCESSION   DAOQLS010000486 DAOQLS010000000
VERSION     DAOQLS010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48761_length_1771_cov_3.993590"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PK362_08725"
     CDS             <1..1004
                     /locus_tag="PK362_08725"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74948.1"
                     /translation="PVGRPTVDEVALEESRVRFRVSVECAPDLATVEWKGLLLKDPGA
                     PVPPEAVDALVEEHRRAAAERRPTAVVVPGADSIVTVVARGKTPAAEAALAGQRPWEI
                     DLGASGADPAVCQALAGAAVGDVREVTIRLPERSGETPSGPHALALTVRAIEQRILPV
                     LDDAWARRLGFGDVASLRSAARAALGRAAQDRRRRELENQIADRLLESHALDLPPTFL
                     EEECRRLWAALRGPAAPWGAADPADAAAVRAEASRRLALTYLLSRIAAEKGLSVTTEE
                     TATVFDREVAGLPGQWRERARGAFEKRREEIGAALLTDRVFDAVLAEARWDSGGEAA"
     gene            1004..1300
                     /locus_tag="PK362_08730"
     CDS             1004..1300
                     /locus_tag="PK362_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNC74949.1"
                     /translation="MPLDKISGAIAAAGAGGPASSPLRKDTGFAATLSQALARADAPA
                     RSAEESVKSLVAGRAESPHDVMIRMEEAHLALLWTVQVRNRVMDAYNEIMRMPL"
     gene            1346..>1771
                     /locus_tag="PK362_08735"
     CDS             1346..>1771
                     /locus_tag="PK362_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74950.1"
                     /translation="MIRGVPRNAQREDALDSLNRFKDSFLAWPTGKKFLAVGLVAVSS
                     LFLHRLSLPMRGLDMVPLYARLSSEDAAAVVEKLRNESVPYALSNNGQTVSVPSGRVY
                     DLRLKMSQEGLPKGDNQGLELLDKAALGASDFVQRVGYQR"
BASE COUNT          278 a          571 c          624 g          298 t
ORIGIN      
        1 aacccgtggg ccgtcccacc gtggacgaag tcgctttgga agagtcccgg gttcgctttc
       61 gggtgtcggt ggaatgcgcc cccgatttgg ccacggtgga atggaaaggg cttcttctca
      121 aagatccggg cgccccggtc ccgcccgagg cggtggacgc cctggtggag gaacaccgcc
      181 gcgcggcggc ggaacgtcgg ccgaccgccg tggtcgtgcc cggcgcggac tcgatcgtga
      241 cggtcgtcgc ccgggggaag acgcccgccg cggaagccgc cttggccggc caacgccctt
      301 gggagatcga tttgggcgct tccggcgccg atcccgctgt ttgccaggcc ctggccgggg
      361 ccgcggtggg cgacgtgcgg gaagtgacga ttcggttgcc cgaacggtcc ggtgaaaccc
      421 cgtccggtcc ccacgcgctg gccctgaccg tgcgggcaat tgaacaacga attcttcccg
      481 tcctcgatga cgcctgggcg cgccgcctgg gatttgggga cgtggcctcg cttcgaagcg
      541 cggcccgcgc cgccctcggg cgtgccgctc aggatcggcg tcgccgggaa ttggaaaacc
      601 aaatcgccga ccggttgctg gaatcccacg ccctggatct tccgcccacc tttctcgagg
      661 aggaatgccg acgtctctgg gcggccctac ggggcccggc ggccccctgg ggcgcggccg
      721 acccggccga tgcggccgcc gtgcgcgccg aagcgtcgcg gcggttggcg ttgacgtatt
      781 tgttgtcccg aatcgccgcc gaaaaaggcc tcagcgtgac cacggaggaa accgccaccg
      841 tttttgaccg cgaagtggcg ggcctccccg ggcaatggcg ggaacgggcc cggggcgcct
      901 ttgaaaaacg acgggaggaa atcggcgccg ccctcctgac ggaccgggtc tttgacgccg
      961 tgctggccga agcccgctgg gattcggggg gggaagcggc gtgatgcctc tggacaagat
     1021 cagcggggcg atcgccgcgg ccggcgcggg aggcccggcg tcttctccgt tgagaaagga
     1081 caccgggttc gccgcgacgt tgagtcaggc cctggcccgg gccgacgctc ccgcccgttc
     1141 ggccgaggaa tcggtgaagt ccctcgtggc cggccgggcc gaaagccccc acgacgtgat
     1201 gattcgaatg gaggaagccc acttggctct gctttggacc gtccaggtcc ggaaccgggt
     1261 gatggacgct tacaacgaaa tcatgcgaat gcctctctaa acgcgaccgg cggctggacc
     1321 tctgtgagga ggccgcggac gccccgtgat ccggggcgtc ccacgcaacg cgcaacggga
     1381 ggacgccttg gactcgctca atcgatttaa agattcgttt ttggcctggc ccacggggaa
     1441 aaagtttctg gcggtgggat tggttgcggt gtcgtcgctt ttcctccacc ggctttcctt
     1501 gcccatgcgc gggctggaca tggttcccct ctacgcgcgg ctgtcgtcgg aggacgcggc
     1561 ggcggtggtg gaaaagctcc gcaacgaaag cgttccctac gctctgtcga acaacggcca
     1621 aaccgtcagc gttccctccg ggcgggtgta cgatctacgc ctcaaaatgt cccaggaagg
     1681 attgcccaag ggcgacaatc agggattgga attgctcgac aaggcggccc tgggcgccag
     1741 cgattttgtc caacgggtgg gttatcaacg g
//
LOCUS       DAOQLS010000487         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_48856_length_1769_cov_5.308051, whole genome shotgun sequence.
ACCESSION   DAOQLS010000487 DAOQLS010000000
VERSION     DAOQLS010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48856_length_1769_cov_5.308051"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1769
                     /gene="dnaK"
                     /locus_tag="PK362_08740"
     CDS             <1..>1769
                     /gene="dnaK"
                     /locus_tag="PK362_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNC74951.1"
                     /translation="LGTSNSAAAVMEGGKPVIIPSAEGTTMGGKAFPSYVAFTKDEQL
                     VVGEPARRQAVSNPEGTVFAFKRKMGQDYKYSIHGKTYTPQQLSAFILQKIKRDAEAY
                     LGEKVEKAVITVPAYFDDNQRQATKDAGQIAGLEVVRLVNEPTAASLAYGLDKIGQAQ
                     KILVFDLGGGTLDVTIMEMGYDKDIKAADFKVLATSGDTQLGGTDMDNVLIDFIAEEF
                     RKENGIDLRKDKLAMQRFKEAAEKAKIELSTVLETEINLPYITGDASGAKHLQMKLSR
                     AKLESLVQPIIDRCKHPMEEALKGAGLSASAIGKVVMVGGPTRMPCVLKFVESFVGRK
                     IEGGVNPMECVALGAAVQAGVLSSDVKDVLLLDVTPLTLGIETLGGVMTPLIKKNTTI
                     PTRASQVFSTAADNQEAVTVHVLQGEREVASGNTSLGHFNLDGIPLAPRGTPQIEVTF
                     DIDANGILNVTALDKGTQKKQHITIEAKNKLSKEEVEKFIKDAELHAEEDKKKKEEIE
                     TKNEADAIVFSTEKALKEYGDKVSQEERMAIERALTDAKEALKGGDLAKIKKTKEDLL
                     KASHKLAEEMYKAASAKAGEGAPG"
BASE COUNT          425 a          574 c          525 g          245 t
ORIGIN      
        1 ctcggaacgt ccaactcggc cgcggccgtc atggagggcg gaaagcccgt catcatcccc
       61 tcggccgaag gcaccaccat ggggggcaaa gccttcccca gctacgtggc cttcaccaag
      121 gacgaacagc tggtcgtggg cgagcccgcc cgccgtcagg ccgtctccaa ccccgagggc
      181 accgttttcg ccttcaagcg caaaatgggc caggactaca aatattccat ccacgggaag
      241 acctacaccc cccaacagct ctcggccttc attctgcaga agatcaaacg cgacgccgaa
      301 gcctatctgg gcgaaaaagt ggagaaggcc gtcatcaccg tgcccgccta cttcgacgac
      361 aaccagcgcc aggccaccaa ggacgccggc cagatcgccg ggttggaagt cgtgcgcctg
      421 gtgaacgagc ccacggcggc cagcctcgcc tacggattgg acaagatcgg ccaggcccag
      481 aaaattctgg tgttcgacct gggcggcggc acgctggacg tgaccatcat ggagatgggc
      541 tacgacaagg acatcaaggc cgcggacttt aaagtgttgg ccacctccgg cgacacccag
      601 ctgggcggca ccgacatgga caacgtcctg atcgacttca tcgccgagga gttccgcaag
      661 gaaaacggca tcgacctgcg caaagacaaa ctggccatgc agcggttcaa agaagccgcc
      721 gaaaaggcca agatcgagct ctcgacggtg cttgaaaccg agatcaacct gccctacatc
      781 acgggcgacg cctccggggc caagcacttg cagatgaagc tctcccgggc gaaactggaa
      841 tccctggtcc agcccatcat cgaccggtgc aaacacccca tggaagaggc gctgaagggc
      901 gcgggccttt cggcctcggc catcggtaag gtggtcatgg tcggcggtcc cacccgcatg
      961 ccctgcgtgc tcaagttcgt ggaatctttc gtgggccgga aaatcgaagg cggcgtgaac
     1021 cccatggagt gcgtggctct gggcgccgcc gtgcaggccg gcgtcttgag ctcggacgtc
     1081 aaagacgtcc tgctcctgga cgtgacgcct ttgaccctgg gcattgaaac cctgggcggc
     1141 gtcatgacgc ccttgatcaa aaagaacacc acgattccca cccgggcctc ccaggtgttc
     1201 tccaccgccg cggacaacca agaggcggtc acggtgcacg tcctccaggg cgagcgcgaa
     1261 gtggcctcgg gcaacacgtc cctgggccat ttcaacctgg acggcattcc cctggcgccc
     1321 cggggcacgc cccagatcga agtcaccttc gacatcgacg ccaacggcat tttgaacgtc
     1381 acggcgctgg acaagggcac ccagaagaaa caacacatca ccatcgaggc caagaacaaa
     1441 ctctccaagg aagaggtgga gaagttcatc aaggacgccg agctccacgc cgaagaggac
     1501 aagaagaaga aagaggagat cgagaccaaa aacgaagccg acgccatcgt cttctcgacg
     1561 gaaaaggccc tcaaggaata cggcgacaag gtctcccagg aagagcgcat ggccatcgaa
     1621 cgcgccctca ccgacgccaa ggaagcgctc aagggcggcg acctggcgaa gatcaaaaag
     1681 accaaggaag accttctcaa ggcctcccac aaactggcgg aggagatgta caaggccgcc
     1741 agcgccaagg cgggcgaagg cgcgccggg
//
LOCUS       DAOQLS010000488         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_49068_length_1766_cov_2.223846, whole genome shotgun sequence.
ACCESSION   DAOQLS010000488 DAOQLS010000000
VERSION     DAOQLS010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49068_length_1766_cov_2.223846"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1425
                     /locus_tag="PK362_08745"
     CDS             61..1425
                     /locus_tag="PK362_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC74952.1"
                     /translation="MADEKFDAIVVGAGPAGTSAALTMAKAGLNVVLLERGDYAGAKN
                     VQGAVMYTKMLDDILPGFWKDPQAPVERSITEQKIWVLSKDSMFQVGFKSEKWLKDPP
                     NCFTVIRANFDRWYAKKAEAAGVQLFTGVTVSEGLKKNGKVVGIKTSEGDEIFADVVI
                     AADGVNSLLGQQLGLLDEWKADEVALGVKEVLALPREKIEDRFNLEQNEGTTIEIFGE
                     NTRGMLGYSFLYTNKESISFGVGAKLSHFQKTGIRPPDLLEYAKAHPMIRRLLKGAKP
                     LEYSAHLIPEGGYNSMPKLFADGILIAGDAAQMVNASHREGSNLAMTAGRLAGETVIE
                     AKKKGDFSAKTLSAYQTKLRDSFVIPDLFDHKDLETMVEKHMDIVAEGPELMAQSLYD
                     YFQVDGRPKRDVQKAILQRLTRNPAVRAVIKSELTLKNEFSLVKMGLKLRLLLWKLTH
                     GGSL"
     gene            1446..1727
                     /locus_tag="PK362_08750"
     CDS             1446..1727
                     /locus_tag="PK362_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010979841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC74953.1"
                     /translation="MNVDEKLGSLTYKADHQAHITIKDNSTCLNKCHDKPCTTVCPAQ
                     VYQWEDGQKKLIVSFENCIECGACRMLCPFDNIQCHWPRGGFGVQYKYG"
BASE COUNT          440 a          465 c          512 g          349 t
ORIGIN      
        1 cccatccaac tggacgaagc cgtgcggcaa gtggttccgg ctccgtccct gtgaggttcc
       61 atggctgacg aaaaattcga cgcgatcgtt gtgggcgcag ggccggcagg gacttccgcg
      121 gcgctaacca tggccaaggc ggggttgaat gtggttctct tggaacgggg cgattacgcc
      181 ggcgccaaga atgtccaggg cgcggtgatg tatacaaaaa tgttggacga tattcttccg
      241 ggcttctgga aggatcccca ggcgccggtg gaacggtcga tcacggaaca gaaaatttgg
      301 gtgctctcga aggactcgat gtttcaagtt gggtttaagt cggagaaatg gttaaaagac
      361 ccgccgaatt gtttcaccgt catccgggcc aactttgacc ggtggtatgc caaaaaagcc
      421 gaagcggcgg gggtccaact ttttaccggc gtgaccgtga gcgaagggct caagaaaaac
      481 gggaaagtgg tcggcatcaa aaccagcgag ggcgacgaaa tctttgccga tgtggtcatc
      541 gcggccgacg gagtcaattc gctcctgggt caacagctgg ggcttttgga cgaatggaaa
      601 gccgacgaag tggccttggg cgtcaaagaa gtgctggcgc tcccgcgtga aaaaatcgaa
      661 gaccggttta atttggaaca aaacgaaggg accaccatcg aaattttcgg cgagaacacc
      721 cgggggatgt tgggctattc cttcctctac acgaataagg aatcaatttc ctttggcgta
      781 ggcgcaaaac tttcccattt ccaaaaaacg ggcataagac caccggacct cctggaatac
      841 gccaaagcgc atccgatgat ccgccgtttg ctaaaagggg cgaagcccct ggaatattcg
      901 gcccatttga ttccggaggg cggctacaat tccatgccga aactgttcgc cgatgggatt
      961 ttgatcgccg gggacgcggc ccagatggtc aacgcctctc accgcgaagg ctcgaatttg
     1021 gccatgaccg ccggtcggtt ggcgggcgag acggtgatcg aggccaagaa gaaaggcgat
     1081 ttcagcgcga agacgctgtc ggcgtaccaa acgaaactgc gggacagttt cgtgatcccc
     1141 gacctcttcg accacaagga tcttgagacg atggtggaaa aacacatgga tattgtggcg
     1201 gaagggccgg aactgatggc ccaatccctc tacgattatt tccaggtgga cggacggccg
     1261 aagcgggacg ttcaaaaagc gattttgcag cgcttaacgc ggaacccggc cgtgcgggcg
     1321 gtgatcaaat ccgagctgac gctcaaaaac gaatttagtc tggtcaaaat ggggctcaaa
     1381 cttcggctcc tgttatggaa actgacccat gggggttccc tgtgagctct gatgccttgc
     1441 ccaaagtgaa tgtggacgaa aagctgggaa gcctgacgta taaggccgac catcaagccc
     1501 acatcaccat caaagacaat tcgacctgct taaacaaatg ccacgacaag ccgtgcacca
     1561 cagtctgccc cgcccaggtc tatcagtggg aagacggcca aaagaagctg atcgtttcct
     1621 tcgaaaactg catcgaatgc ggcgcctgcc gcatgctctg ccccttcgac aacatccaat
     1681 gccactggcc ccgcggcggc ttcggcgtcc aatacaaata cggctgagca attttctatt
     1741 taacgaaaaa ggccctggtt ccctca
//
LOCUS       DAOQLS010000489         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_49329_length_1760_cov_4.203519, whole genome shotgun sequence.
ACCESSION   DAOQLS010000489 DAOQLS010000000
VERSION     DAOQLS010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49329_length_1760_cov_4.203519"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK362_08755"
     CDS             <1..200
                     /locus_tag="PK362_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HNC74954.1"
                     /translation="RLIEAAGLKGTRVGDARISPKHANFIENVGHASAADVHALLALV
                     QKTVREKSGVDLELEVWPVGD"
     gene            204..1136
                     /locus_tag="PK362_08760"
     CDS             204..1136
                     /locus_tag="PK362_08760"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNC74955.1"
                     /translation="MTPSIPAALRRRRIALLQGGRSAERPISLKSGRAVSAALRRLGL
                     PHQSLDIVSNLPTALRRHKIDLAFLVTHGPFGEDGRLQGLLDLLNIPYTGSGVLASAL
                     AMHKPSAKALFQNAGLPTAPWCLRRRGEPADRPPFPGPWVVKPASQGSAVGVRVVRRA
                     GEWAPALRKSWAVEPEALVERYLRGPEYTVGVLDDHPLPVVQIIPKHAFYDFYSKYAV
                     GGSRHVVPAPLPPRRVERLQRLAVAAHRALGCRHVSRVDLIWSDRPYLLEVNTLPGMT
                     DTSLLPDAARAAGLSFDDLVLRLLTLALRDAAAA"
     gene            1133..>1760
                     /locus_tag="PK362_08765"
     CDS             1133..>1760
                     /locus_tag="PK362_08765"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNC74956.1"
                     /translation="MKRRRHKVTVRRSHAAYRWRTFAESLAALIRAILFLGSAAALAV
                     GAHWFWTRSQSLAIGAVRLEGVPPVGFRDALGLQPGQPLFGFHRRDLEKRLRAAFPAL
                     ASVRLERQWDRTVRVTVVPRIPRARVLADGRWRGVDDSGALFPLPDEGAGLPVFALPD
                     DSAVRANALAFLKGLRNTKEGWTEGLYKIKMSSDGDAVLYLAGDTPVQW"
BASE COUNT          249 a          664 c          534 g          313 t
ORIGIN      
        1 cccgcctcat cgaggcggcg ggcctcaagg gaacgcgggt gggcgatgcg cggatctctc
       61 cgaaacacgc caatttcatt gaaaacgtcg gccacgcttc ggccgccgac gtccacgcgc
      121 tgttggccct cgtccaaaaa accgtccggg aaaaaagcgg cgtcgacctc gaactggaag
      181 tctggcccgt cggggattaa cgcgtgaccc cgtcgattcc ggccgccctc cggcgccgcc
      241 gtatcgcctt gctccagggg ggacgttccg ccgaacgtcc gatttcccta aaaagcggcc
      301 gcgccgtttc ggccgccctc cggcgcctgg gactgcccca ccaatccctc gacatcgttt
      361 ccaacctccc gacggccctt cgccgccata aaatcgacct cgcttttctg gtgacccacg
      421 gccccttcgg ggaggacgga cgactccagg gtcttctcga tttattgaac atcccttaca
      481 cggggagcgg cgtcctggcc agcgccctcg ccatgcacaa accctcggcc aaggcgctct
      541 ttcaaaacgc gggccttccc acggccccct ggtgccttcg acggcggggc gaacccgccg
      601 accgtccgcc gttccccggc ccttgggtcg tcaagcccgc cagccagggg tcggccgtcg
      661 gggtgcgcgt cgttcggcgg gccggcgaat gggcgccggc ccttcgaaaa tcctgggccg
      721 tggaacccga ggccttggtg gaacgctacc tccgcggccc cgaatacacc gtgggcgttt
      781 tggacgacca cccgttgccc gtggtccaga tcattcccaa acacgccttt tacgattttt
      841 actccaaata cgctgtcggc ggttcccgcc acgtggtgcc ggcgcccctg cccccccgtc
      901 gagtggaacg ccttcaacgc ctcgccgtcg ccgctcaccg ggccctgggg tgccgccacg
      961 tgagccgggt cgatttgatt tggagcgacc ggccctattt gctggaagtc aacacgctcc
     1021 cgggaatgac cgacacgtcg ctcctgccgg acgccgcccg ggccgccggc ctgtccttcg
     1081 acgatttggt ccttcgcctg ttgaccctgg cccttcggga cgccgccgcg gcatgaagcg
     1141 gcgccgccac aaagtcacgg tgcgccgcag ccacgcggcc taccgttggc ggaccttcgc
     1201 ggagtccctg gccgccctga tccgggccat cctgttcctc gggtccgccg ccgccctggc
     1261 ggtgggcgcc cactggtttt ggacccgctc ccaatccctg gccatcgggg cggttcgtct
     1321 cgagggcgtc ccgcccgtcg gcttccggga cgctctcggg ctccagcccg gtcagcccct
     1381 cttcggattt catcgtcggg atcttgaaaa acggctccgc gccgccttcc ccgccctcgc
     1441 ctccgtccga ctggagcggc aatgggaccg aaccgttcgg gtcaccgtcg ttccccggat
     1501 cccccgggcc cgggtcctgg ccgacggtcg gtggcggggc gtcgacgatt ccggcgccct
     1561 tttcccgttg ccggacgaag gcgcgggcct tccggtgttc gccctcccgg acgattcggc
     1621 cgtccgcgcc aacgccctgg ccttcttgaa gggccttcga aacaccaaag aaggctggac
     1681 ggaaggcctc tacaaaatta aaatgtcttc cgacggggac gccgtccttt acctggccgg
     1741 ggacaccccg gtccaatggg
//
LOCUS       DAOQLS010000490         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_49388_length_1759_cov_3.587441, whole genome shotgun sequence.
ACCESSION   DAOQLS010000490 DAOQLS010000000
VERSION     DAOQLS010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49388_length_1759_cov_3.587441"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1759)
                     /locus_tag="PK362_08770"
     CDS             complement(<1..>1759)
                     /locus_tag="PK362_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74957.1"
                     /translation="LFDFVSILHTQQISLWSPLHFTIEPLARAAGGDAGLFVDLLDAT
                     RDLLMDLRDRSVDTTRTEQYGVGAAAAAFVGDGLNLLRSLRFARPLVAKNQDPGWLLQ
                     LTLPAMKSDAGFEKKLNALSAFLAPLLERGVFTPYPLAVGLGTVAKIMSAEDPWARAL
                     DLLGAMVKSLDAAKVVGLFRGGLEELPTDLIPPDRLIRALTVAVALADRGVDPAPTLR
                     RGALSPESLAVAEKLTAEGGDPAPVLAGDFRIYEALGLLNRGGERLIGLALALGTRRG
                     KGERFFEEAIPAVAALEAETPGGGEDLLRFSELMIQKGLDPTVAILFGFPRAMSARPA
                     PPGLRELVVEFAGALAEASADPTAALRFALRPLAEIAGGNGDRFRELSAGILRLILSL
                     VKKGADVQDTLFHDIQRMAEEGATSEVFLAFLRRLERVFDLWSARGADLGALAERGLP
                     GLAQASVGKPWLMDIVFDRLEELGRADKIRDGLALLEHCLAVASDAAGSDAVLFRAAL
                     AALERSVARLPAELTAVAPPAAGALAGGNVARLEEILGVVADRAPVPVPAELTEALPL
                     LARLSDGADSLGSLMDRSLA"
BASE COUNT          336 a          645 c          546 g          232 t
ORIGIN      
        1 gggccaagga ccggtccatc aaggaaccca gggagtccgc gccgtcggac aaccgcgcca
       61 acagcggaag cgcctccgtc aactcggccg gcacgggtac gggcgcgcgg tcggccacca
      121 cgcccagaat ctcctccaac cgggccacgt tccctccggc caaggcgccg gccgccgggg
      181 gggccacggc cgtcagttcg gcggggagtc gcgcgacgga ccgctccagg gcggccaggg
      241 ccgcccggaa cagcaccgcg tccgaacccg ccgcgtcgct cgccacggcc aggcaatgct
      301 ccagcaatgc cagaccgtcg cggattttgt ccgcccggcc cagttcctcc aaccggtcga
      361 agacgatgtc catcagccaa ggcttcccaa cggaagcctg ggccaagccg ggcaatcccc
      421 gttcggccag ggcccccaga tccgcgcccc gggccgacca aagatcgaac acccgctcca
      481 atcgccgcag gaaggccaga aagacttccg aggtcgcccc ttcctcggcc atccgttgaa
      541 tgtcgtggaa gagcgtgtcc tggacgtcgg cccccttctt taccagggac aaaatgagtc
      601 gcagaatccc cgccgacaat tcccgaaagc gatccccgtt tcctccggcg atttccgcca
      661 agggccgcag agcgaaacgg agggccgccg tgggatcggc cgaggcttcg gccagcgccc
      721 cggcaaattc caccaccaac tcccgcaacc ccgggggcgc cggacgggcc gacatcgccc
      781 gggggaaccc aaagaggatc gccaccgtcg ggtccagtcc tttttggatc atcaattccg
      841 aaaaacggag aaggtcctcc cctccccccg gcgtttcggc ttcgagggcg gccacggcgg
      901 gaatcgcctc ctcgaaaaat ctctcgcctt tgccccgccg cgtgcccagg gccagggcca
      961 aaccgatcaa ccgttccccg ccccggttca gcaaccccag ggcctcgtag attcggaagt
     1021 cccccgccaa caccggggcg gggtcgcctc cctcggccgt caatttttcg gccacggcca
     1081 gggattcggg cgagagggcc ccgcggcgaa gggtcggcgc ggggtccacc ccccggtccg
     1141 ccagggccac ggcgacggtc aacgcccgaa tcaaccggtc gggcgggatc aaatcggtcg
     1201 gcaattcctc caggcccccg cgaaagagcc ccaccacctt ggccgcgtcc agggatttca
     1261 ccatcgcgcc cagaagatcc agggcccgag cccagggatc ctccgccgac atgattttgg
     1321 ccaccgttcc caaccccacc gccaggggat agggcgtgaa aacgccccgt tccaggagcg
     1381 gcgccaaaaa ggccgacagg gcgttcagct ttttctcgaa acccgcgtcg cttttcatgg
     1441 ccggcagggt caactgcaat aaccatcccg ggtcctgatt cttggccacc aaggggcggg
     1501 cgaagcgcag cgatcgaagg aggttcaacc cgtcgcccac aaaggccgcg gcggccgcgc
     1561 ccacaccgta ttgctcggtg cgggtcgtgt cgacggaccg gtcccgcagg tccatcagca
     1621 aatcccgggt cgcgtcgagc aaatcgacga aaagcccggc gtccccgccc gcggcccggg
     1681 ccagaggttc gatggtgaaa tggagcggag accagaggga aatctgctgg gtgtggagga
     1741 tggaaacgaa atcgaacag
//
LOCUS       DAOQLS010000491         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_49555_length_1756_cov_4.098178, whole genome shotgun sequence.
ACCESSION   DAOQLS010000491 DAOQLS010000000
VERSION     DAOQLS010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49555_length_1756_cov_4.098178"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PK362_08775"
     CDS             complement(<1..1351)
                     /locus_tag="PK362_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNC74958.1"
                     /translation="MIVLGLSFFYHDAAAALLVNGRLVAAAEEERFSRRKHDSDFPER
                     AIRFCLAQAGLTVGDVDHVVFYEKPLLKFQRLLTTLAAHAPRTAGTFREAMVSWLGEK
                     LWIKPLIQDRLGLPADKILFCEHHLSHAASAFFCSPFKEAAVLTIDGVGEWSTATLGR
                     GTARWEDGGANRLDLFSEIRFPHSLGLFYSAFTAFLGFEVNEGEYKVMGMAPYGTPRH
                     VDKVWKLLRLSEDGSFELDLSYFSFQHDRWRTYSPKFERLFGPARNPRARFVTETTSL
                     CDDTTPPAAAEINRNQYYADVAASVQRVTEEIVLKMARHLHRVTGLKSLCVAGGVALN
                     SVANHRLLKETPFEDIYIQPAAGDAGGALGAALYVEHAVAGNPRRFVMDRADWGRSNS
                     PSEAADFYLSIGVGFEEYSDENRLLDRVAGLLLDGRVTGWVQGRFEWGPRALGHRSIL
                     "
     gene            1516..>1756
                     /locus_tag="PK362_08780"
     CDS             1516..>1756
                     /locus_tag="PK362_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74959.1"
                     /translation="MEIADGKGAKARVKVNEVCIGNVGSPEDAANISRVIGMTAGLHK
                     SIPGYTVHRNCASGMEAISQGWLKIASGEADVMMVG"
BASE COUNT          378 a          516 c          553 g          309 t
ORIGIN      
        1 ccaggatcga ccggtggccc aaagcccggg gcccccattc gaagcgcccc tggacccacc
       61 ccgtcacccg accgtccagg aggagaccgg ccacgcgatc caacaaccga ttttcatcgg
      121 aatattcctc gaaacccaca ccgatcgata aataaaaatc ggcggcctcg gacggagagt
      181 tcgatcgacc ccaatcggcc cggtccatga cgaaccgtcg gggatttccc gccaccgcgt
      241 gttcgacgta gagcgccgcg cccagggccc cgcccgcgtc gcccgcggcg ggctggatgt
      301 agatgtcctc gaagggcgtt tcttttagga ggcggtggtt cgccaccgag ttgagagcga
      361 cgccgcccgc cacgcaaagg gattttaagc ccgtgacccg gtggagatgg cgggccattt
      421 tcaacacgat ttcctcggtg acccgctgaa cgctggcggc tacgtcagcg taatactgat
      481 tccgattgat ttccgcggcg gccggcgggg tcgtatcgtc gcagagactc gtggtctcgg
      541 tcacaaaccg cgcccggggg ttccgggccg ggccgaagag ccgctcgaat tttggcgagt
      601 aggtgcgcca gcgatcgtgt tggaaggaga aataggacag gtccaactcg aaactgccgt
      661 cctcgctgag ccggagaagt ttccacactt tgtccacgtg gcggggcgta ccgtaggggg
      721 ccatgcccat gaccttgtat tccccctcgt tgacttcaaa tcccaaaaac gccgtaaagg
      781 ccgaataaaa caaccccagg gagtggggaa agcgaatctc ggaaaacaag tccaaccgat
      841 tggcgcctcc gtcctcccaa cgggcggttc cccggcccag ggtggcggtg ctccattcgc
      901 cgaccccgtc aatggtcaac accgccgcct ccttgaacgg ggaacaaaag aacgcgctgg
      961 ccgcgtggga gaggtggtgt tcgcaaaaaa ggattttatc ggcgggaaga ccgagccggt
     1021 cttgaatcaa cggcttgatc caaagctttt cccccagcca ggacaccatg gcctcgcgaa
     1081 aagtcccggc cgtgcggggc gcgtgggcgg ccagggtggt cagaagccgc tgaaatttta
     1141 ggagcggctt ttcgtaaaac accacgtggt cgacgtcccc gacggttaag ccggcctggg
     1201 ccaggcagaa tcgaatcgct ctctccggaa aatccgagtc gtgctttcgg cgggagaaac
     1261 gctcttcctc ggcggcggcg accagccggc cgttcaccag aagggcggcc gcggcgtcgt
     1321 gatagaaaaa actaagtccc agaacgatca tcaggattgt cggcgaaggt cggcccaggc
     1381 gcgatcccgc gtttgccggg ggacccacgc cgggccggac ggcccaacct gccgacggcg
     1441 acgtcccagc cttccggcga tcatcgccac gggggcgaac aggaggaggt agaacagggt
     1501 gagaatcagt ttggtttgga aatcgccgat ggcaaggggg ctaaggctcg cgtaaaagtg
     1561 aatgaagttt gtattggtaa tgtgggcagc cccgaagatg cagcgaatat ttcgcgcgtg
     1621 attggaatga cagcgggtct acacaaatca attcctggct atacagttca cagaaactgc
     1681 gcttcaggaa tggaggcgat ttctcaaggc tggttaaaaa ttgcttccgg cgaggctgat
     1741 gtgatgatgg tgggag
//
LOCUS       DAOQLS010000492         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_49607_length_1755_cov_4.214706, whole genome shotgun sequence.
ACCESSION   DAOQLS010000492 DAOQLS010000000
VERSION     DAOQLS010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49607_length_1755_cov_4.214706"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PK362_08785"
     CDS             complement(<1..1410)
                     /locus_tag="PK362_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74960.1"
                     /translation="MDNTPTLRLLLADRDAGFTRIFQERFRALAGHTVTIDTCTAGAD
                     LVERFYAQPYDLVFLDLALPGLDPKDLLSRLSQTSLPLPVVALSSEHNTRPVVEAMKL
                     GVLDHLMKEDFATLDLAGFVGRLRETFRLRRENAELLQINQMKNDFLATISHELRTPL
                     TSILGLSEILLAGRMGPLVPRQSESLKKIIEQSQNLAKLINQLLDVQDIVQERMRLDF
                     RPLSLAEITQARGEAVEPLFKQKGVSLRVEPMDDRLIFQGDREAVDKVLEHVLLNALK
                     FTPAKGLVLVSAKALPPRSIQLSVLDTGQGIPNEALPYVFQKFFHADQSLTRAYGGLG
                     LGLAYCKHVVEALGGRIWVESAGVGKGTRVNMTFPQAGLKGVDNGKSLKTVLWVDDNP
                     NMLELIEVGFTALPTNVKLLTCRSGTAALESANTRPPDLIVLDIMMPDMNGLEVLDRL
                     QKNEKTRKVPVMVVSGYREA"
     gene            complement(1458..>1755)
                     /locus_tag="PK362_08790"
     CDS             complement(1458..>1755)
                     /locus_tag="PK362_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HNC74961.1"
                     /translation="LKQWLGDRVTRQELLAAWIDRFSKSYRRFAADGFAPFRPAYAAR
                     SWLRGRAVRFQTAAGAATGRAAGVDATGRLMVETPRGTVAVAAGEVSLLRLNSR"
BASE COUNT          320 a          528 c          580 g          327 t
ORIGIN      
        1 ggcctcgcgg tagccgctca ccaccatgac ggggactttg cgggtctttt cgtttttctg
       61 aagtcggtcc aaaacttcca gaccgttcat gtccggcatc atgatgtcca gaacgattaa
      121 atccgggggg cgcgtgttgg cggattccaa ggcggcggtg cccgagcggc aggtgagcag
      181 tttgacgttg gtcggaaggg ccgtgaaccc cacctcgatc aactccagca tgttcgggtt
      241 gtcgtccacc cagaggacgg ttttgagact cttgccgtta tcaacgccct tgagtccggc
      301 ctgcgggaaa gtcatgttca cgcgggtgcc cttgcccacc cccgccgatt ccacccagat
      361 gcgtccgccg agggcttcca cgacgtgttt gcagtaggcc aggcccaagc ccaatccgcc
      421 gtaggcccgg gtgagggatt gatccgcgtg gaaaaacttt tgaaatacgt agggcagcgc
      481 ctcgttgggg atgccctggc ccgtgtccag aacgctcaat tggatggacc ggggcggcag
      541 ggctttggcg gacaccaaca ccaacccctt ggccggcgtg aatttgaggg cgttcaggag
      601 cacgtgctcc agcaccttgt ccacggcttc ccgatccccc tggaagatca accggtcgtc
      661 catgggttcc acccggaggg aaaccccttt ttgcttaaaa agcggttcga cggcctcgcc
      721 ccgggcctgg gtgatttccg ccagggagag cggccggaaa tccaggcgca ttcgttcctg
      781 aacgatgtcc tgaacgtcga gcaattggtt gatgagtttc gccaggttct gggactgctc
      841 gatgattttc ttcaaacttt cgctttgccg gggcaccagg gggcccatgc ggccggccaa
      901 cagaatttcg ctgagtccga ggatcgacgt caggggcgtg cggagctcgt gggaaatggt
      961 cgccaggaaa tcgtttttca tctggttgat ctgcaggagt tccgcgtttt cccggcgcag
     1021 acggaaggtt tcccgaaggc ggccgacgaa gcccgccagg tccagcgtcg cgaaatcctc
     1081 cttcatcaaa tgatccagaa cgcccaactt catggcttcg accacgggcc gggtgttgtg
     1141 ttcgctcgac agggcgacca cgggcagggg cagcgaggtt tgggacaggc ggcttaaaag
     1201 gtccttggga tcgaggcccg gcagggcgag gtccaaaaac accaggtcgt agggttgggc
     1261 gtaaaagcgt tccaccaagt cggccccggc ggtgcaggtg tcgattgtga cggtgtggcc
     1321 ggccagggcg cgaaaccgct cctgaaaaat ccgggtgaaa ccggcgtccc ggtcggccaa
     1381 aagcaatcgt agggtgggcg tgttgtccat ggcgctcgtc gggccgaaac ccgcggctcc
     1441 ttaagtatat aggcgcttca gcgggaattc aaccggagaa gggagacttc cccggccgcg
     1501 accgcgaccg tcccccgggg ggtctcgacc atcagtcggc cggtcgcgtc caccccggcc
     1561 gcgcggcccg tggcggcgcc cgcggcggtt tggaagcgga ccgcccggcc ccgaagccag
     1621 gaacgcgcgg cgtaggccgg gcggaacggg gcgaaaccgt cggcggcaaa ccggcggtag
     1681 gattttgaaa agcgatcgat ccaggccgcc agcagttcct ggcgggtgac ccggtcgccc
     1741 agccactgct tcagg
//
LOCUS       DAOQLS010000493         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_49726_length_1753_cov_2.816843, whole genome shotgun sequence.
ACCESSION   DAOQLS010000493 DAOQLS010000000
VERSION     DAOQLS010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49726_length_1753_cov_2.816843"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PK362_08795"
     CDS             complement(<1..681)
                     /locus_tag="PK362_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74962.1"
                     /translation="MKLTLAQRLYGISLGGVLFTLLVGAVVYLGFSGLERDLRDMVTT
                     TASLRDHMEANTMHETVRADVYAAFQAVSEEERAAVAKDMNECLNHLYERLEQNKAHG
                     YNAVVREVAQESEPQVRRYGQKAQSLLALLKTRPEEAKRRLNEFDAVYDETSIVLSNM
                     GDKLEEAIRTTERRRAAIAQFGRSFSAALCFSAPVALILVVLLTLKSFRRTLASAAGS
                     LQEMAMSDV"
     gene            complement(692..1111)
                     /locus_tag="PK362_08800"
     CDS             complement(692..1111)
                     /locus_tag="PK362_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNC74963.1"
                     /translation="MKRLCTFLIASTRYALPVVSVQEILRAPVLTPVPLAPPFVKGIF
                     NLRGHILTSVDLARVVGAVSEARTTGGPQVVVRHGVETVGLAVDKVGDVETVDENRAA
                     TPPANLAPAARAVVREAHALPNALLLELDIEKILELA"
     gene            complement(1108..>1753)
                     /locus_tag="PK362_08805"
     CDS             complement(1108..>1753)
                     /locus_tag="PK362_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNC74964.1"
                     /translation="LAVEHIVDTEEIVVKPLGRHLKSMNLFEGATVLGDGAVVLILDI
                     PGLADHGGVAATELAAPAFGDALSAPKPAGEPHLLLAGPDDGRLALPLSRVLRLERIS
                     LAALERVGSEEILPYRQGPLRIVRLTRLLPERRKSARRLSPENPDQAHVVVCVSGGQP
                     VGVAVHRILDIVDEIPADTRPGGRPGVRSTVIVRGRVTELLDLDALLAGPAGSP"
BASE COUNT          316 a          598 c          551 g          288 t
ORIGIN      
        1 cacgtccgac atggccattt cctgcagaga cccggcggcg gacgccaggg tgcgccggaa
       61 ggatttcagc gtcaacagca cgacgaggat gagcgccacc ggcgccgaga aacacaacgc
      121 ggcggaaaag gacctaccga attgggcgat ggcggcccgg cgccgttccg tcgtccgaat
      181 ggcttcttcc agcttgtcgc ccatgttgga cagcacgatg gaggtttcat cgtagaccgc
      241 gtcgaattcg ttgagcctgc gcttggcctc ctccggccgg gttttcagaa gggccaggag
      301 gctttgggct ttctgtccgt accgccggac ctggggttcg ctttcctggg cgacttcccg
      361 aaccaccgcg ttgtatccgt gggccttgtt ttgctccagc cgttcataga ggtggttgag
      421 gcattcgttc atgtccttgg ccaccgcggc gcgttcctcc tccgacaccg cttgaaaggc
      481 ggcgtagacg tccgcccgaa cggtttcgtg catggtgttg gcctccatgt ggtcccgcag
      541 ggaagcggtg gtggtcacca tgtcccgcaa atcccgttcc aaccccgaaa acccgaggta
      601 caccacggcc cccaccagga gggtgaacag aaccccgccc aggctgatgc cgtacaaccg
      661 ctgcgccaac gtgagtttca tagaggcctc ctcaggccaa ttccaaaatt ttttcgatgt
      721 cgagctccag caacaacgcg ttcggcaagg cgtgcgcttc ccgcaccacg gcccgcgccg
      781 ccggcgccag attggccggc ggggtcgccg cccggttttc gtcgacggtt tcgacgtccc
      841 cgactttgtc cacggcgagc ccgacggttt cgacgccgtg gcggaccacc acctggggcc
      901 ccccggtcgt ccgcgcctcc gaaacggcgc ccacgacccg cgcgagatcc acggacgtca
      961 aaatgtgacc ccgcaaatta aagattccct tcacgaaggg cggggccaag ggcaccggtg
     1021 ttaaaaccgg cgcgcgcagg atttcctgca cggacaccac gggcagggcg taacgcgtcg
     1081 acgcgatcaa aaacgtgcaa agccgtttca cggcgatccc gcgggcccgg ccagcagagc
     1141 gtccaagtcc agcagttccg tcacgcgtcc ccggacaatc acggtcgaac gcaccccggg
     1201 gcgccccccc gggcgagtgt ccgcggggat ttcgtcgacg atatccaaaa ttcggtgcac
     1261 ggccacgccg acgggctgtc cccccgaaac gcaaacgacc acgtgggcct ggtccgggtt
     1321 ttccggggag agccgccggg ccgatttccg acgctcgggc aacaatcggg tcaggcggac
     1381 gatccgcagg ggcccctgcc ggtagggaag gatttcctcg gaccccaccc gttccagggc
     1441 cgccagggaa atccgctcca gccgaagaac ccgggacagg ggcagggcca accggccgtc
     1501 gtccggcccg gccaggagaa ggtggggttc gcccgcgggt ttcggcgcgg acagggcgtc
     1561 gccgaaggcg ggcgcggcca gctcggtggc ggccacaccg ccgtggtcgg ccaatcccgg
     1621 aatgtccaga ataagaacca ccgcgccgtc gcccagcacc gtggctccct cgaacaaatt
     1681 cattgatttc agatggcgcc ccaggggttt aacaacgatt tcctcggtgt ccacgatgtg
     1741 ttccaccgcc aag
//
LOCUS       DAOQLS010000494         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_49986_length_1748_cov_3.724158, whole genome shotgun sequence.
ACCESSION   DAOQLS010000494 DAOQLS010000000
VERSION     DAOQLS010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49986_length_1748_cov_3.724158"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PK362_08810"
     CDS             complement(<1..746)
                     /locus_tag="PK362_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNC74965.1"
                     /translation="MSNRFTERAQRVILIAQEEAKRLNHDYVGTEHLLLGLIALGEGV
                     AAQVMANLGVDLRRVRAEIEKIVGTGDNVMLLGEIPFTPRAKKVLELAVEEAQNMGHN
                     YVGTEHLLLGLIREEEGVAARVLENIGVRLDVVREEVISLLGEGQSGAQGAPGGAQPP
                     AHHQTPARTKSKTPTLDEFGRDLTQMAKDGKLDPVIGRETEIERVIQILLRRTKNNPV
                     LIGDPGVGKTAIVEGLAQRLSSDEAPELLQG"
     gene            complement(881..1741)
                     /gene="ilvE"
                     /locus_tag="PK362_08815"
     CDS             complement(881..1741)
                     /gene="ilvE"
                     /locus_tag="PK362_08815"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNC74966.1"
                     /translation="MFLNGRLVPKEQAVVSVFDHGLLYGDGVFEGIRAYNGRVFRLQE
                     HLERLYRSARAIVLDIGMPLADMEKAVLDTLRANKLKDAYIRLVVTRGVGDLGLDPKK
                     CPKATVFIIADRIALYPPECYTEGLEVNTVSTRRNSSQALNPNIKSLNYLNNILAKIE
                     AGLSGAREAIMLSLEGYVAECTGDNIFFIKGNRLVTPPTVAGALEGITRAVVWGLASG
                     AGLVPEEMLFTPFDLFTADEVFLTGTAAEVIPVVRIDARTIGAGRPGPKTQKLIQAFR
                     ELAGREGTPI"
BASE COUNT          306 a          547 c          532 g          363 t
ORIGIN      
        1 ccctgaagga gctcgggggc ttcgtcggag gaaaggcgct gggccagtcc ttccacgatg
       61 gcggtcttgc cgaccccggg gtccccgatc aacaccgggt tgtttttcgt ccgccggagc
      121 aaaatttgga tgacgcgctc aatttcggtt tcccggccga tgacgggatc cagcttgccg
      181 tccttggcca tttgggtgag gtcccggccg aattcgtcca aggtcggggt tttggatttc
      241 gtgcgggcgg gggtctgatg gtgcgccggg ggctgggcgc cgcccggggc gccctgagcg
      301 ccggattgtc cttcgcccag cagggaaatc acttcctcgc ggacgacgtc caaacggacg
      361 ccgatgtttt ccagaacccg ggccgccacg ccctcctctt cccgaatgag ccccaggagg
      421 aggtgttcgg tgcccacgta attgtggccc atgttctggg cttcctcgac ggccagttcc
      481 aaaacttttt tggcccgggg cgtgaagggg atttcgccca acaacatcac gttgtcgccc
      541 gtgcccacga tcttctcgat ttccgcccga acgcgccgca aatcgacgcc caaattggcc
      601 atcacctggg ccgccacccc ttcgcccagg gcgatcaaac ccaacaacaa atgttccgtc
      661 cccacgtaat cgtggttcag gcgtttggcc tcttcctggg cgatcagaat gactcgttgc
      721 gcgcgttcgg tgaatcggtt ggacatctcg acctccgcga tcgacggctg tttcgacttc
      781 gctgaattct agaccctttt ccgggggctg tcaatttcta cttcggccgg aacccgcttt
      841 ttcttgaacg acttccggga ccgaaagttc ccgacggaat ttaaatcggg gttccctcgc
      901 ggcccgccaa ctcccgaaag gcttggatca atttctgcgt tttgggcccg ggccgaccgg
      961 caccgatggt gcgggcgtcg atgcggacca cggggatcac ttccgccgcc gtgcccgtca
     1021 agaaaacttc gtcggccgtg aacaggtcga agggcgtgaa gagcatttcc tcgggcacca
     1081 acccggcccc ggacgccagg ccccaaacca cggcccgggt gatgccttcc agcgccccgg
     1141 ccaccgtggg gggagtcacc agacggttcc ctttgatgaa aaagatgttg tcgcccgtgc
     1201 attcggccac gtagccctcc agggagagca tgatggcttc ccgggcgccg gacaaacccg
     1261 cttcgatttt cgccaggatg ttgttcaaat agttcagcga tttgatgttc gggttaagcg
     1321 cttggctgga attgcgccgg gtcgaaaccg tgttgacctc caacccctcg gtgtagcatt
     1381 ccggcgggta aagggcgatg cggtccgcga tgatgaacac ggtggctttg gggcattttt
     1441 tcgggtccag cccgagatcg cccacgcccc gggtcacgac aagccggatg taggcgtcct
     1501 tcagtttgtt ggcgcgcaga gtgtccaaca cggccttttc catgtccgcg aggggcattc
     1561 cgatgtccag cacgatggcc cgggcggaac ggtacagtcg ttccaaatgt tcctgaagcc
     1621 ggaaaacccg gccgttgtag gcccggatgc cttcgaaaac cccgtcgccg tagagaaggc
     1681 cgtggtcgaa caccgacaca acggcctgtt ccttgggcac gaggcgcccg ttcagaaaaa
     1741 tctggagg
//
LOCUS       DAOQLS010000495         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_50037_length_1747_cov_4.852246, whole genome shotgun sequence.
ACCESSION   DAOQLS010000495 DAOQLS010000000
VERSION     DAOQLS010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50037_length_1747_cov_4.852246"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1747
                     /locus_tag="PK362_08820"
     CDS             <1..>1747
                     /locus_tag="PK362_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74967.1"
                     /translation="RLARTPDERALAARDRRLYLAGKLVDFSLSPAEWSEWRSAPAAG
                     EVDDPRFRPFADFYREAEARDAAMTVKTLQAVRSKSARNPILVTGGFHAESMDRRLAE
                     AGCVVVQAVPRLSRVDPAAGSSYLAVFAQERTPLEKLFAGEKRFLTPDPLEGERQMPF
                     AVGAALDSPEAARALYNAFPRPLVLEGVERTDDAPGPGLRADFAGGSRLGFAWRFTGD
                     GQRTFVQTPLKRGRTVASILGALMSAGWLAASGPATPAAPVNPPATIVNPAVRLNEIQ
                     SLLSAQKAALNPDEAAAVQRLLSMERSMRALSASGEMQVPEDFYQLLHSVNAMLRPRD
                     LILFPNSAPPEFALNLLRILRVSRLYPDVYYVEPLDGYQGLKTVEGVEEHGRYALGFT
                     IVHRNVNRRSADNVVRVLKAGDDLPPDYRGLRMGEVYRRQWPRGADAVGKVADDLFES
                     AAIHETEHRLNGGERDALLAEIALGPVPRFKLESVNFMYASRFDEKDTHPYFRAVSWV
                     FSRFCEETGRKSPREIGQWLTEVPDLDLRLLAVRMYVTVKGRALLRGPAAVLAPRLGL
                     PVEDVDRLLAAQAEAM"
BASE COUNT          278 a          604 c          549 g          316 t
ORIGIN      
        1 cgcctcgccc gcacccccga cgaacgagct ctcgcggccc gggaccgccg actttacctc
       61 gcgggaaaac tggtcgactt ctcactttcc cccgcggaat ggtccgaatg gcgttccgcc
      121 cccgccgcgg gggaggtcga cgacccccgc ttccggccct ttgcggattt ttaccgggag
      181 gccgaagccc gggacgccgc catgacggtc aaaaccctcc aagccgtccg gtcgaagtcc
      241 gcccggaacc ccatcttagt gacgggcggc tttcacgccg aatccatgga ccgtcgactg
      301 gccgaggcgg ggtgcgtggt ggttcaggcc gtgccccgtc tctcccgcgt ggatcccgcc
      361 gcgggatcct cctatctcgc cgtgttcgcc caggaacgga cccccctgga gaaactgttc
      421 gccggcgaaa aacggttcct caccccggat ccgttggagg gcgaacggca aatgcccttt
      481 gccgtcggcg ccgccctgga ctcgcccgaa gcggcccggg ccctgtacaa cgcgttcccc
      541 cggcccctcg ttttggaagg ggtcgagcgg acggacgacg cccccggccc cggcctgagg
      601 gcggatttcg ccggtggttc ccgtttggga ttcgcctggc gtttcacggg cgacggccag
      661 cggacctttg ttcaaacccc gttgaagcgg ggccgcacgg tggcgtcgat cctgggcgct
      721 ttgatgtccg cgggttggct ggcggcgtcg gggcccgcga cgccggccgc tcccgtgaat
      781 ccgcccgcca cgatcgtcaa ccccgccgtc cgcctcaacg aaattcaatc gcttctctcc
      841 gcccagaagg ccgcgctgaa tcccgacgaa gccgcggccg ttcaacgcct cttgtcgatg
      901 gaacgatcca tgcgcgccct gagcgcctcc ggcgaaatgc aggtgccgga ggatttctac
      961 cagcttctcc acagcgtcaa cgccatgctt cgcccgcggg atttgatcct ctttcccaac
     1021 agcgcgccgc cggagttcgc cttgaacctg ttgcgcatcc ttcgggtctc ccgcctttat
     1081 cccgacgtct attacgtgga acccctcgac ggctaccaag ggttgaaaac ggtggaaggc
     1141 gtggaggaac acggccgtta cgccctgggg ttcaccatcg tccaccgaaa cgtgaatcgc
     1201 cgctcggcgg acaacgtggt tcgggttctg aaggccgggg acgatcttcc gccggattac
     1261 cggggacttc gaatggggga ggtctatcgg cgtcaatggc cccgcggcgc ggacgcggtg
     1321 gggaaagtgg ccgatgattt gtttgaatcg gcggcgatcc acgaaaccga acaccgcctg
     1381 aacggcggcg aacgcgacgc cctattggcc gaaatcgccc tggggcccgt tccacgcttc
     1441 aaactggaat cggtcaattt catgtacgcc tcgcggttcg acgaaaagga cacccacccc
     1501 tactttcgag ccgtgtcctg ggtgttcagc cgtttttgtg aggaaacggg acggaaaagt
     1561 ccccgggaaa tcggccaatg gttgacggaa gtccccgatc tggaccttcg ccttttggcc
     1621 gttcgaatgt acgtgaccgt taagggacga gcccttctcc ggggaccggc cgccgtcctc
     1681 gccccgcgcc tggggctccc ggtggaggac gtcgaccgcc tgttggcggc ccaggccgag
     1741 gcgatgc
//
LOCUS       DAOQLS010000496         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_50256_length_1743_cov_3.734005, whole genome shotgun sequence.
ACCESSION   DAOQLS010000496 DAOQLS010000000
VERSION     DAOQLS010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50256_length_1743_cov_3.734005"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK362_08825"
     CDS             complement(<1..538)
                     /locus_tag="PK362_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNC74968.1"
                     /translation="MTGNAAPPTVVIVGRPNVGKSTLFNRLCESRRALTSPIAGTTRD
                     WLEGDCHWNGRLFRVIDTGGYSPGNDDVLTSVRAQVEEWIRRADAVVWVVDAGEGLTA
                     SDQSFARLLRPQSKRVIVVVNKSDDARRDAATAEFARLGFSPLLGISATHGRRINGLL
                     ETLEEKIPRPAAAGEFSLP"
     gene            complement(535..1080)
                     /locus_tag="PK362_08830"
     CDS             complement(535..1080)
                     /locus_tag="PK362_08830"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNC74969.1"
                     /translation="MTKPPKRALTWANRITLLRLLLTPVLVIGLLSGARGWPVLIFLL
                     AALSDVLDGAVARWRGEQTTLGKYLDPIADKLLLSSSYLALAHLQVFPLWVFVVVFSR
                     DLFILIGWNVIYILTRNFTVEPRWLGKGTTFLQMATVVLALTVPGAGILPWALGATVL
                     MTTLSAIDYVWLGSRKLGELG"
     gene            complement(1077..>1743)
                     /locus_tag="PK362_08835"
     CDS             complement(1077..>1743)
                     /locus_tag="PK362_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNC74970.1"
                     /translation="LVALVGYTNAGKSTLLNALRENPAGAVYADDKLFATLDPTTRRA
                     RLPSGRAALFVDTVGFIQHLPHHLVAAFHATLEEARNADLLVHVIDAGSAEWISQRDV
                     VREVLKTLAADALPQIELFNKTDALTAAQRAVHQRESRLLASATTGEGLGEFLTRVEE
                     RLDEVLKEREFLVPHARRDLMPRIHEAGHLLEKIPEAEGTRVRVRLDEKNWGQLRKEL
                     GLP"
BASE COUNT          344 a          577 c          538 g          284 t
ORIGIN      
        1 ctggaagaga aaattccccg gccgccgccg gccggggaat tttctcttcc agcgtttcca
       61 gcaaaccgtt gatccgccgc ccgtgcgtcg ccgaaatgcc caacaggggc gaaaacccca
      121 gccgggcgaa ttcggccgtg gccgcgtccc gtcgcgcgtc gtcggattta ttcaccacca
      181 cgatcacgcg tttcgattgg ggccgaagaa ggcgggcgaa ggactggtcg gaggccgtga
      241 gcccttcccc ggcgtccacg acccaaacga cggcgtcggc ccggcggatc cactcctcca
      301 cctgggcccg aaccgaggtc agcacgtcgt cgttgcccgg ggaatagccg ccggtgtcga
      361 tgacgcggaa aagccgtccg ttccaatggc agtcgccctc cagccagtcc cgggtggtgc
      421 cggcgatggg ggacgtcagc gcgcgccggc tttcgcaaag gcggttgaac aaagtcgact
      481 tccccacgtt ggggcggccg acgatgacga cggtgggcgg cgcggcgttt ccggtcatcc
      541 caattccccc agcttccggg acccgagcca cacgtaatcg atggccgaga gcgtcgtcat
      601 caacaccgtg gcgcccagcg cccaaggcaa gattcccgcg cccggcacgg tgagggccag
      661 gaccacggtc gccatttgca gaaaggtcgt gcctttgccg agccagcggg gctccacggt
      721 gaaattgcgg gtcaaaatat aaatcacgtt ccacccgatc aggatgaaca gatcccggga
      781 gaaaaccacc acgaacaccc acagcggaaa aacctgcaag tgcgccaagg ccaagtagga
      841 ggacgacagc aaaagcttgt ccgcgatggg atccagatat ttgcccaggg tggtttgttc
      901 cccccgccag cgggccaccg cgccgtccaa aacgtcggac aacgccgcca aaaggaaaat
      961 caagaccggc cagccgcggg cgccgctcag gaggccgatg accaacacgg gagtcaacaa
     1021 taggcgcaga agggtgatgc ggttcgccca agttaaggcg cgtttcggcg gtttcgtcac
     1081 ggcagtccca attcctttct caattgaccc caatttttct cgtccaggcg aacgcgcacg
     1141 cgggttccct ccgcctccgg gattttctcc agcaaatgcc ccgcctcgtg aatgcgcggc
     1201 atcaaatccc gccgggcgtg gggcaccaaa aattcccgct ctttgagcac ttcgtccaag
     1261 cgttcctcga cccgggtcag gaattccccg agcccttcgc ccgtggtggc gctggccaag
     1321 agccgggatt ccctctggtg gaccgcccgc tgggcggccg tcagggcgtc ggttttattg
     1381 aacagctcga tttgaggaag cgcgtcggcg gccagggttt tgaggacctc ccgcacgacg
     1441 tcgcgctgcg aaatccattc cgcgctcccg gcgtcgatca cgtgaaccaa caaatcggcg
     1501 ttccgcgcct cttcgagggt ggcgtgaaag gcggccacca gatggtgggg caaatgctga
     1561 atgaacccca cggtgtccac gaacagggcc gcccgaccgc tcggcaaacg ggcccgccgg
     1621 gtggtcggat ccagcgtggc aaaaagcttg tcatcggcgt aaaccgcccc ggcggggttt
     1681 tcccggagag cgttcaaaag cgttgatttc ccggcgttcg tgtaacccac cagggccacc
     1741 agc
//
LOCUS       DAOQLS010000497         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_50888_length_1732_cov_3.592725, whole genome shotgun sequence.
ACCESSION   DAOQLS010000497 DAOQLS010000000
VERSION     DAOQLS010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50888_length_1732_cov_3.592725"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PK362_08840"
     CDS             <1..694
                     /locus_tag="PK362_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC74971.1"
                     /translation="LLKNKSGNLQTDDLTRLERIKANGFHLLTLVNDVLDLSRVEAGR
                     MPLLLSDVNLDILVLETISEMESRISEKKEIRLLTDLPPRLAAFRTDERKFKQVLINL
                     MNNALKFTEQGSITIGVVADAATARPTRLEVRDTGIGIPEEKREVIFEAFQQADNTTA
                     RQFGGSGLGLTITAALLKLMGHRLELDSEVGRGSTFRIVFGTPEDETPASEARTVSPS
                     SADNSAKDRTAQ"
     gene            698..1078
                     /locus_tag="PK362_08845"
     CDS             698..1078
                     /locus_tag="PK362_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74972.1"
                     /translation="MKPKLDVVEDNDDNRLLLRGILEDLFELREFETGPDALRAIQAD
                     RPDIVLLDISLPGMDGVEVLVRLRENPTTRNLPVVALTAHAMVGDREKFLAFGFDDYV
                     SKPIVDESDLFAAIRRCLARTPAP"
     gene            1075..>1732
                     /locus_tag="PK362_08850"
     CDS             1075..>1732
                     /locus_tag="PK362_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC74973.1"
                     /translation="MNELTDMRGLYREILRDRRRELEVVLDKYRASSGATEEELRRLA
                     HKIAGSGSAFGFPRITEAARAAEHAPEGGVAGAVDELVAAMAAVLESQRRCPVLVVAP
                     ESPWWTEFGARLTSTGRPVVRARSAGEAGPLLNGEEVALIVLDLLLPDQDGRRFLAEL
                     REDGLNSCVPVFVLAAHVNDDLRNECLAYGSVRCFQKPVSILTLVQAVDEKVKAKPES
                     D"
BASE COUNT          349 a          526 c          539 g          318 t
ORIGIN      
        1 cctgctaaaa aacaaatccg gaaatctcca gaccgacgat ttaacccggc tggaacgaat
       61 caaagccaac ggatttcacc tcttgacgct ggtcaacgac gtcctggacc tctcccgggt
      121 ggaagcgggc cgcatgccgc ttttgttgtc ggacgtcaac ctcgacatcc tggtgttgga
      181 aacgatttcg gaaatggaat cccgcatttc ggagaagaag gaaatccgat tgttgaccga
      241 cctgccgccc cgcttggccg cgttccggac cgacgaacga aaattcaaac aagtcctgat
      301 caacctcatg aacaacgcct tgaaattcac cgaacagggc tccatcacca tcggcgtggt
      361 ggccgacgcg gcaaccgccc gccccacccg tctggaggtc cgggacacgg ggatcggaat
      421 tccggaagaa aagcgggaag tcattttcga agcgtttcaa caggccgata acaccacggc
      481 ccgccagttc gggggatccg ggttgggatt gaccatcacg gcggcgctgt tgaagctgat
      541 gggccatcgc ctggagctgg acagcgaagt cgggcggggc tcgaccttcc gcattgtatt
      601 tggcaccccg gaggacgaaa cgcccgcgtc cgaggcccgg acagtttcac cgtcttcggc
      661 cgataattcg gccaaggaca ggaccgccca ataacccatg aagcccaaac tcgacgtggt
      721 cgaagacaac gacgacaatc gcctcctttt aagggggatt ctcgaggatt tgttcgaact
      781 tcgggaattc gaaacgggcc ccgacgcgtt gcgggccatt caagccgacc ggccggacat
      841 cgttctcctg gacatatccc tccccggcat ggacggcgtg gaagtgctgg tccgcctgcg
      901 cgagaacccg acgacacgaa acctcccggt ggtcgcgttg accgcccacg ccatggtcgg
      961 cgatcgtgag aaatttctcg ccttcgggtt tgacgattac gtttcgaaac cgatcgtgga
     1021 cgaatcggac ctgttcgcgg ccatccgtcg ctgtttggcg aggaccccgg cgccatgaac
     1081 gaattgaccg atatgcgcgg gttgtaccgg gagatccttc gggatcgccg ccgggagttg
     1141 gaagtcgtcc tcgataaata tcgggcctcc tccggggcca ccgaggagga actccgccga
     1201 ttggcccaca aaatcgcggg gtcgggaagc gccttcggtt ttccccgaat cacggaggcc
     1261 gcccgggccg cggaacacgc cccggagggg ggggtggccg gagccgtgga cgagttggtg
     1321 gccgccatgg cggcggtgct cgaaagccaa cgtcggtgtc cggtgttggt ggtggctccc
     1381 gagtcccctt ggtggacgga attcggcgcg cgcctgactt ccacgggacg tcccgtggtt
     1441 cgggcccgca gcgccggcga agccggcccg ctcctgaacg gcgaggaggt cgccctcatc
     1501 gtgcttgatc tcctccttcc cgaccaggac ggacggaggt tcctggccga acttcgagag
     1561 gacgggttga acagttgcgt gcccgtgttc gtgttggcgg cccacgtgaa cgacgacttg
     1621 cggaacgaat gcctcgcgta tggatccgtc cgttgctttc aaaaaccggt atccattctc
     1681 acgctcgttc aggccgtgga tgagaaagtg aaggccaaac cggaaagcga tg
//
LOCUS       DAOQLS010000498         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_50927_length_1732_cov_1.914729, whole genome shotgun sequence.
ACCESSION   DAOQLS010000498 DAOQLS010000000
VERSION     DAOQLS010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50927_length_1732_cov_1.914729"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PK362_08855"
     CDS             <1..484
                     /locus_tag="PK362_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNC74974.1"
                     /translation="DTVERAKSAALSPCSGAIFGMGESADDILDVAYRLRETGVDSIP
                     INFLIPVPGTTLQGKNDLTPNRCLKILCLFRFLNPAMELRIAGGRELHLRSLQTLGLY
                     VANSIFVGDYLTTKGQSAAADRQMVRDMGFEVVGDPSPAEPPPALSDQVEIVTRGSRQ
                     "
     gene            492..1211
                     /locus_tag="PK362_08860"
     CDS             492..1211
                     /locus_tag="PK362_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC74975.1"
                     /translation="MNVLITGASRGIGRALALAFGGAGHAVAVNYRSRKEEAESVAAD
                     LKRRGTSADLFPGDVSDPAQAKALVDGVVNKWGRIDGLINNAGIVRDRTLLKMSGEEW
                     QDVIDTNLSGPFYCLQAAAHHMARQKSGFIFNIASILASRGGVGCANYVAAKAGLVGL
                     TKAAARELGRFNVRVNAVLPGFHPTEMSENIPAEQKGRVLAQHVLGRSTDLGELSRFV
                     LVLAEMSTVSGQVFNVDSRIL"
BASE COUNT          306 a          509 c          547 g          370 t
ORIGIN      
        1 cgacaccgtt gagcgggcca aatcggcggc cttgtccccg tgttccgggg cgattttcgg
       61 aatgggcgaa tccgccgacg acattttaga tgtggcctat cgcctgcgcg agaccggcgt
      121 ggactcgatc cccatcaact tcctcatccc tgttcccggc acaacgttgc aagggaaaaa
      181 tgacctgacg ccgaaccgat gcctcaaaat cctgtgcctt tttcggttcc tcaacccggc
      241 catggagttg cgcatcgcgg gcggtcggga acttcacttg cgctctcttc aaacgctggg
      301 gctctacgtg gccaattcca ttttcgtcgg agattactta accacgaagg gccaatccgc
      361 cgcggccgat cgccaaatgg ttcgcgacat ggggttcgag gtggtggggg atccctcccc
      421 cgccgaaccg ccgcccgcgc tgtccgatca ggtcgaaatc gtcacccgcg ggtcccggca
      481 ataaccttcc catgaatgtg ttgatcaccg gcgcgagccg aggaattggg cgagcattgg
      541 ctcttgcgtt cggcggggcg ggccacgcag tggccgtcaa ttatcgttcc cgcaaagagg
      601 aggccgaatc cgtggcggcc gacctgaaaa gacggggaac gtccgcggat ctttttcccg
      661 gcgacgtgtc cgatcctgcc caggccaagg cgttggtgga cggcgttgtg aataaatggg
      721 gtcggatcga cgggctgatc aataacgcgg gcattgttcg ggatcggacc ctcctaaaaa
      781 tgagcgggga agagtggcag gatgtcatcg acaccaacct ctccggaccg ttctattgct
      841 tgcaggcggc ggcccatcac atggcccgtc aaaaaagcgg gtttatcttc aacatcgcgt
      901 cgatcttggc atcccggggc ggcgtggggt gcgccaatta tgtggccgcc aaagccggat
      961 tggtcggcct gaccaaagcc gccgcacggg aattggggcg gttcaatgtg cgggtcaacg
     1021 cggttctgcc gggttttcac cccaccgaga tgtccgagaa tattccggct gaacaaaaag
     1081 ggcgggtgct cgcccagcat gtgttgggac ggtccaccga tttgggggaa ctctcccggt
     1141 ttgtgttggt tttggcggag atgtccactg tttccggcca agtgttcaac gtggattccc
     1201 ggattctgtg aacgagaaaa agcaagcggt cgttaccggc atcggcctcg tttgtcccct
     1261 cggggcggac cgagaatccg gttggcgttc gttgatcgac ggccgttcgg ccgtttctcg
     1321 ggacggagcc agagtcccgg gaaattggtt tttctcgata aattcccctc tccctccagt
     1381 gggattgccg cgcaccattt cgttagcgct ccaggcttgg aaggaagcct ggcaagacgc
     1441 gggtttttcc gattccggca acgggccgga tcccgatcgt gtgtcctgtg tggtgagttc
     1501 cagcaaaccg attttcggat tcggagacga cccatcttgc cagtcccccg acgtcgttgc
     1561 ctccgccgtg gctcacgccg cgggcgcgga ggggatggtg ttgaacatcg cttccgcttg
     1621 cgccactggc gcccagtcgc ttttaacggc ggcggggtgg atcgaagaag gccgggcgga
     1681 cgtggttttg gcgggcgcgg ccgaaagtgc cctccacccg ctttatctct cg
//
LOCUS       DAOQLS010000499         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_50969_length_1731_cov_3.312649, whole genome shotgun sequence.
ACCESSION   DAOQLS010000499 DAOQLS010000000
VERSION     DAOQLS010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50969_length_1731_cov_3.312649"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1731
                     /locus_tag="PK362_08865"
     CDS             <1..>1731
                     /locus_tag="PK362_08865"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNC74976.1"
                     /translation="LEDLTLWDGTVRRLWMSGEVGPRKWDFDLRAVGMEAETLVAIAN
                     LDWSVTGRWNARVRGRGTPADPGVEADIRGRDGRLGPVPYDRLEADVRWSGSTVTVRQ
                     LRVARSNGYLLTGEAHGPAVGGSAGGGGVGARLRLQDGELQILQDMWPVCKKARGTFS
                     GELNIDSGKGGLRSAGYFEIRDGQMTNRSYARRVNDLALRLLLVDDRLTVESGTARIG
                     AGRAVLRGEVLLPRDAPPEYNLWLETLGDRGVEVEVPQLSVPPGPVFSRLPIFQESLK
                     GVSRGEPVFVLHATGPQGRHVITADVTLNNSQFTYPPAKGAFHRVPGPRFWRDFWREA
                     AWDVRFHTGKDTWYRNEYVNVEIDGNLALSGRPGAWLANGKIGARQGVINYLGQNFIV
                     SQGSFEVETDTRPGLTRGVYPYISGVAEKTVAAVDARGFANDDVVTMVVDRARLGEIQ
                     PRFLSRNSPGLKSDRVAMRALGLSGEDQLTQSDRDQLFRAGLVQLVGASAAPLATRLA
                     QNFGIYMINTIYEPPETTETASPTSPNQSPSATAAPARDVSSYLRGAGASARIRLTDR
                     LFGVYKVKLD"
BASE COUNT          289 a          539 c          598 g          305 t
ORIGIN      
        1 cctggaggat ttgaccctct gggacgggac cgtccgacgt ctttggatgt ccggcgaggt
       61 gggaccccga aaatgggatt ttgaccttcg ggcggtgggg atggaagcgg aaacgctcgt
      121 cgccatcgcc aacttggatt ggtccgtcac gggccggtgg aacgcccggg tccggggccg
      181 cggcaccccc gccgatccgg gggtggaggc cgatattcgg gggcgggacg gccggttggg
      241 acccgtgccc tacgatcgcc tggaggcgga cgtccgctgg tccggatcga ccgtcaccgt
      301 tcgccagctg cgcgtggccc ggtccaacgg atatttattg accggcgagg cccacggccc
      361 ggccgtcgga ggttcggcgg ggggcggggg cgtgggggcc cgccttcgat tgcaggacgg
      421 cgaattgcaa atccttcaag acatgtggcc cgtgtgcaaa aaagcccggg gaaccttttc
      481 cggggaactg aacatcgact cggggaaagg cggccttcgg tcggccgggt actttgaaat
      541 acgggacggt caaatgacca accgttccta cgcgcggcgc gtcaacgact tggcgcttcg
      601 gctgttgttg gtggacgacc ggctgaccgt ggagtcgggg accgcccgga tcggcgcggg
      661 ccgggcggtc cttcggggag aggtcctcct tccccgggac gcgcccccgg agtacaacct
      721 gtggttggaa acgttggggg accggggggt ggaggtcgaa gtcccgcaat tgtccgtgcc
      781 gccgggcccc gtgttcagtc ggcttccgat ttttcaggaa tccctgaagg gcgtgtcccg
      841 gggggagccg gtcttcgttc tccacgccac cggcccccag gggcgtcacg tgatcacggc
      901 ggacgtcacc ctgaacaatt cccagtttac ctacccgccg gccaagggag ccttccaccg
      961 ggtgccgggc ccccgttttt ggcgggattt ctggcgcgaa gcggcctggg acgtccggtt
     1021 tcacacggga aaggacacct ggtaccgaaa cgaatacgtc aacgtggaaa tcgacggcaa
     1081 cctggccctg tccgggcggc cgggcgcctg gttggccaac ggcaaaatcg gcgcccgcca
     1141 gggagtcatc aactatttgg ggcaaaattt tatcgtgagc cagggctcct ttgaagtgga
     1201 aaccgacacc cggcccgggc tgacgcgggg ggtctacccc tacatctccg gcgtggcgga
     1261 aaaaaccgtc gcggcggtgg acgcccgggg gttcgccaac gacgacgtcg tgaccatggt
     1321 ggtggaccgc gcccgcctgg gggagattca accccgtttc ctttcccgga acagccccgg
     1381 tctcaaatcc gaccgggtgg ccatgcgggc cctgggcctt tcgggggaag atcaactgac
     1441 ccaatccgat cgggaccaac tttttcgggc cgggctcgtt caattggtgg gggccagcgc
     1501 ggcgcccctc gccacgcggt tggctcaaaa cttcggaatt tatatgatca acacgattta
     1561 cgagcccccg gaaacgaccg agaccgcttc cccaacgagc ccgaaccaaa gcccgtccgc
     1621 caccgccgcc ccggcccggg atgtgtcgtc gtatttgcgc ggcgcggggg cttcggcccg
     1681 tatccgttta accgatcggt tgttcggcgt ctacaaagtg aaattggacg a
//
LOCUS       DAOQLS010000500         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_51176_length_1727_cov_4.164474, whole genome shotgun sequence.
ACCESSION   DAOQLS010000500 DAOQLS010000000
VERSION     DAOQLS010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51176_length_1727_cov_4.164474"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PK362_08870"
     CDS             <1..536
                     /locus_tag="PK362_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNC74977.1"
                     /translation="GGPPIARFDFPRQSAGDLLCLSDFVLPASDGKMDYVALFVVTCG
                     QGILELSREWREKGDYLKSHALQAIAIESAEALAELLHDRIRSMWGIGDPKDMTLQQK
                     LQTKYRGIRVSFGYPACPNLEDQAQLFALLDPAKHIGVGLTENFMMEPEASVSALVFH
                     HPEARYFSVLGEETAVS"
     gene            596..1720
                     /locus_tag="PK362_08875"
     CDS             596..1720
                     /locus_tag="PK362_08875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC74978.1"
                     /translation="MWAGIAAVYFLLRLNLLPIPFDRDEGFFGYGGQVLLRGGALYRD
                     MVDHKPPLVFYLYSLILRVLPPTPVGVHVFLHAYNFLTTLALYRLARRWVDRGPARSA
                     ALAYAVVSSAMAVQGFTASTEMFLLLPLTLAAWFSLSKSPSLSRRWAAGALAAGAFWI
                     KSQAAPAAFFIGWGILVAGFRAGGVRLAFRRGLGFLAGAAATTGLVLWPFCRAGFLAD
                     LGYWTFSYNRLYAQGAGFWSTSVPGTVRNLGRLSLEHPGVLVLAAIGLGAARDRQKIH
                     TRWPAGFALFSFLGVLPGYAFPHYLALLTPSMALLAGVGWKAVEGKWKGSSAGLRTAV
                     LFGALAVVPGIFHSGYYFRWSGEETSLRVFDFMVSMARSK"
BASE COUNT          237 a          534 c          558 g          398 t
ORIGIN      
        1 ggggcgggcc gccaatcgcc cgctttgatt ttccccgtca atcggccggc gatctgctct
       61 gtctgagcga tttcgtcctt cccgcttcgg acgggaaaat ggattacgtc gccttgttcg
      121 tcgtgacctg cggccagggg atcctggaac tttcccggga atggcgggag aagggcgact
      181 atctcaagtc ccacgcgctc caggccatcg ccatcgaatc ggccgaggcc ctggcggagt
      241 tgttgcacga ccggatccgg tccatgtggg gcatcggcga tcccaaggac atgaccctcc
      301 agcagaagct tcaaaccaag taccggggga tccgcgtgag cttcggctac ccggcttgcc
      361 cgaacctgga ggaccaggcc caactcttcg cccttctgga cccggccaag cacatcggcg
      421 tgggcctcac ggaaaacttc atgatggagc cggaggccag cgtgtcggcc ctggtgttcc
      481 accaccccga agcgcggtat ttcagcgtgt tgggcgaaga aacggccgtt tcttgaggga
      541 tcgcccaccg gaaagaagcc cccggaccct ttccggggcg atgggcgggg gaccgatgtg
      601 ggcgggaatt gcggccgttt attttctgtt gcggctcaac ctgcttccca tcccctttga
      661 ccgggacgaa gggtttttcg gctacggcgg ccaggtgctc cttcggggcg gcgcccttta
      721 ccgggacatg gtcgaccaca aaccgcccct ggttttttat ctttattccc tgattcttcg
      781 ggttttgccg cccacgcccg tgggcgtcca cgtttttctc cacgcctaca attttctcac
      841 gaccctcgct ctttaccgat tggcccggcg gtgggtcgac cgggggcccg cccggtcggc
      901 ggctttggcc tacgcggtcg tcagttcggc catggccgtc caggggttca cggcgtccac
      961 ggagatgttt ctgcttttgc ccctgacctt ggcggcctgg ttttcacttt cgaaatcccc
     1021 ttctctctcc cgacgctggg cggccggcgc cctggcggcc ggcgcctttt ggatcaaaag
     1081 ccaggcggcc ccggcggcgt tttttatcgg ttgggggatc ctcgtcgccg gttttcgcgc
     1141 ggggggcgtc cgcctcgctt tccgacgagg tctggggttt ttggccgggg ccgcggcgac
     1201 cacggggctc gtcctgtggc cgttttgccg agcggggttt ctcgcggatc tggggtattg
     1261 gactttttcc tacaaccgcc tttacgccca gggcgcgggg ttttggtcga cgtcggttcc
     1321 ggggacggtt cgaaacctgg gccggctctc tctggagcat ccgggcgttc tcgttttggc
     1381 ggcgatcgga ctcggggcgg cgcgggaccg gcaaaaaatc cacacccggt ggccggcggg
     1441 gttcgcgctg ttttcctttc tcggcgttct gcccggttac gcctttcccc attacctggc
     1501 cctcctgacg ccaagcatgg ctctgctcgc cggcgtcggt tggaaagccg tcgaggggaa
     1561 atggaaaggt tcgtcggcgg gccttcgaac ggcggtgctt ttcggcgcgt tggcggtggt
     1621 ccccgggatt tttcattccg ggtattattt ccgatggtcc ggggaggaga cgagcttgcg
     1681 ggtttttgat ttcatggtat caatggccag atcaaaataa tcgcaaa
//
LOCUS       DAOQLS010000501         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_51364_length_1724_cov_3.270821, whole genome shotgun sequence.
ACCESSION   DAOQLS010000501 DAOQLS010000000
VERSION     DAOQLS010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51364_length_1724_cov_3.270821"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="PK362_08880"
     CDS             complement(<1..1230)
                     /locus_tag="PK362_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC74979.1"
                     /translation="MIHDAIVVGAGPAGTAAALTMARAGLSVVLLERGEYPGAKNVQG
                     AVLYAKMLEDIVPGFWKDPQNPMERPITEQRIWVLSQDSGLQVGFKSDRWLQEPHNCY
                     TIIRCNFDRWFSKKAEEAGAQLFTGVTVREGLKKDGKVVGVKTSEGDELYADVVVAAD
                     GVNSLLAQQLGLMDEWKADEVALGVKEVLALPREKLEDRFNLERGEGTTIEMFGDITQ
                     GMLGYAFLYTNKESISFGVGAKLSHLQKTGIRPPDLLEAAKNHPLVRRVLRDAKPLEY
                     SAHLIPEGGYNSMPPLYADGFLIAGDAAQMVNPSHREGSNLAMAAGRMAGETVIEAKA
                     KGDFSSKTLSNYQKKLRESFVIPDLYDHRNLESEIEKHMDFVAEAPTLLAQSAMDYFA
                     VDGRPRRDVQAAIIKRAL"
     gene            complement(1309..>1724)
                     /locus_tag="PK362_08885"
     CDS             complement(1309..>1724)
                     /locus_tag="PK362_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585461.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNC74980.1"
                     /translation="NRCLCLFATHYFELTRLAEEYREAANVHLDAVEHKDRIVFLHAL
                     EEGPASRSYGLQVAQLAGVPPGVIRSARRKLQDLETAAVPQGDLFASAAAPVTHEPVA
                     AASHPAVERLAQIDPDSMSPREALEALYALRALLG"
BASE COUNT          272 a          531 c          580 g          341 t
ORIGIN      
        1 aagcgcccgc ttgatgatgg cggcctggac gtcccggcgg gggcggccgt cgacggcgaa
       61 ataatccatg gccgactggg ccaacagggt cggcgcctcg gccacaaaat ccatgtgctt
      121 ttcgatctcg gactcgagat ttcgatggtc gtagaggtcc ggaatgacga aggactcccg
      181 aagttttttt tgataattcg aaagggtttt ggaggaaaaa tcccctttgg ctttggcttc
      241 aatgacggtt tctcccgcca ttcgaccggc ggccatggcc aggttggacc cttcccggtg
      301 ggaaggattg accatttggg ccgcgtcccc ggcgattaaa aaaccgtcgg cgtagagggg
      361 cggcatggag ttgtagccgc cctcgggaat caaatgggcc gagtattcca ggggtttggc
      421 gtcgcggagc acccgccgga ccaggggatg gttcttggcg gcctcgagca gatcgggcgg
      481 ccggatgccg gttttctgca aatgggagag tttcgcgccg accccgaagg agatgctttc
      541 cttgttcgtg taaaggaacg cgtatcccaa catgccctgg gtgatgtccc cgaacatttc
      601 gatggtggtg ccttcgcccc gttcgaggtt gaaacgatct tcgagttttt cccggggcag
      661 ggccaacacc tccttgaccc ccagagccac ttcgtcggcc ttccattcgt ccatcaagcc
      721 gagctgttga gccaaaaggg agttcacgcc gtccgcggcc acgaccacgt cggcgtagag
      781 ttcgtcgcct tcgctggttt tgaccccgac caccttgccg tcctttttca ggccttcgcg
      841 gacggtcacg cccgtgaaca actgggcccc ggcttcctcg gctttcttgg aaaaccagcg
      901 gtcgaagttg caccggatga tcgtgtagca attgtggggt tcctggagcc agcggtcgga
      961 tttaaatccg acctggaggc cggaatcctg ggagagcacc cagatgcgct gctcggtgat
     1021 ggggcgttcc atggggtttt gggggtcttt ccaaaacccg gggacgatgt cctcgagcat
     1081 tttggcgtag aggaccgcgc cctggacgtt tttggcgccg gggtattccc cccgctcgag
     1141 caggacgacg ctgaggcccg cccgggccat ggtgagggcc gccgcggtgc ccgcgggacc
     1201 cgcgcccacc acgatggcgt cgtggatcac ggtcggaggc gtgcttgcgg gttctgcgca
     1261 gggcgccgtc ccgccgttgc gtcggcctgg ggccgccggc cgggcgcgtc agccgagcag
     1321 cgctcgcaac gcatagaggg cttccagggc ttcgcgcggg ctcatggaat ccggatcgat
     1381 ctgcgcgagc cgttccacgg cggggtggga tgccgccgcc accggttcgt gggtgaccgg
     1441 cgccgccgcg ctggcgaaga gatccccctg tggcacggcg gccgtctcca gatcctgcag
     1501 cttccggcgc gcactgcgta tgacgccggg cggcacgccc gccagctgcg ccacctgcag
     1561 cccgtagctg cgggacgccg gaccttcttc cagcgcgtgc aggaacacga tgcggtcctt
     1621 gtgttccacg gcgtccaggt gcacgttggc ggcttcgcgg tattcctccg ccagccgtgt
     1681 cagctcgaag tagtgcgtcg cgaagaggca caggcagcgg ttgc
//
LOCUS       DAOQLS010000502         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_51546_length_1721_cov_2.702881, whole genome shotgun sequence.
ACCESSION   DAOQLS010000502 DAOQLS010000000
VERSION     DAOQLS010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51546_length_1721_cov_2.702881"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..342
                     /locus_tag="PK362_08890"
     tRNA            269..342
                     /locus_tag="PK362_08890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:303..305,aa:Arg,seq:tct)
     gene            409..852
                     /locus_tag="PK362_08895"
     CDS             409..852
                     /locus_tag="PK362_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74981.1"
                     /translation="MEAVVKLLAGLVMLAVSLSYLYRPSYILKVNELARGVLFNDAYV
                     LHYRRRWGMPLFVASIVFLYSGMANLKVQPTGPEAASYGEMDEAYRAFYTRRYADAER
                     LCRGILQLHPRDPHAGFLLRQTLKAMEEGEPKDHLDSSKGNPHRP"
     gene            860..>1721
                     /locus_tag="PK362_08900"
     CDS             860..>1721
                     /locus_tag="PK362_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNC74982.1"
                     /translation="MTTARGLLQGLRLPVGWEAELYVETGDDRDIGWADGRPKDCQVT
                     RSSGAALRVGRDGRRGFASGTDVSPEGVDRLWERARAAAELTPRDSARRLPGPAPISA
                     GRAPSPDRALLQAPVVLLERRLAALEKNLLKSDRRVRRALSLSFSETRGEWAVVSTRG
                     VGVAQPVGSVSFGLELLGESRGDTQVAWGSGEKIRWDRLDVRAIAADVRQRLLDSFGA
                     EPLSTGRWPVVFEPRVGVDLWELLSQAVAGDAVQKGRSFLGTKLGKPVGSSRVTIRDD
                     GRLPGGLASGP"
BASE COUNT          270 a          544 c          572 g          335 t
ORIGIN      
        1 aaaggaaata cgaataagga cattcttgta actgggggaa taagaaagta tatggagacc
       61 taaatggaaa atccgaaacc agttgtcact tgcgttgcta aaataatctt cttctatggt
      121 ttattggttg gaattccaat ttggggcccg cggtttccgc gctagcgcgc tccaaccatt
      181 tcctcggccc ccggcctcgg tcatagcctg ccggagggcg acgagcccag ctcgggaaca
      241 ttgggtttat tggttagaat tcgatttggg gcccgtagct caggtggata gagcacctgc
      301 cttctaagca gggggtcggc ggttcgaatc cgcccgggcc cggatattgc tctggccctt
      361 cgaggggccg ggcggaacgc gtcgcaacgt ctaagggaga tcctttccat ggaagccgtc
      421 gtaaaactgc tcgcggggtt ggtcatgttg gcggtgagtc tttcctacct ttaccgccca
      481 tcgtacattc tcaaggttaa tgaattggcc cggggcgttc tcttcaacga cgcctacgtc
      541 cttcattacc ggcgtcgctg gggcatgccg ctgtttgtgg cttcgatcgt tttcctttac
      601 tccggcatgg cgaatttgaa agttcagccg accggccccg aggccgcctc ctacggcgaa
      661 atggacgagg cttaccgggc tttttacacc cgtcgatacg ccgacgccga gcgcctctgc
      721 cgcggaatcc tacagctcca tccccgggac ccccacgccg ggtttctcct ccgtcaaacg
      781 ctgaaggcca tggaggaggg ggaaccgaaa gaccacctcg attcctccaa gggaaatccc
      841 catcgcccct aaatcccttt tgaccaccgc ccggggcctg ctccagggcc tgcgcctgcc
      901 cgtcgggtgg gaggcggaac tttatgtgga aacgggcgac gaccgggaca tcggctgggc
      961 cgacggccgc ccgaaggatt gccaggtgac ccggtcgtcg ggcgcggccc tgcgggtggg
     1021 gcgggacggc cgccggggtt tcgcctccgg caccgacgtc tcgcccgagg gcgtcgaccg
     1081 gctctgggaa cgggcccggg ccgcggccga gttgaccccc cgggattccg ctcgccgcct
     1141 tccggggccc gctccgattt ccgccggccg cgcgccgtcg ccggaccgcg ccctgctcca
     1201 ggccccggtg gtcctcctgg agcgccgcct ggccgccctg gaaaaaaacc ttttgaaatc
     1261 cgatcggcgg gtgcgccgcg ccctgagcct ttcgttctcc gaaacccggg gggaatgggc
     1321 cgtcgtgtcc acccgggggg tgggggtggc ccagcccgtg ggttccgtgt ccttcgggtt
     1381 ggagctcctg ggggaatccc ggggggacac ccaggtggcc tggggctccg gcgagaaaat
     1441 ccgctgggac cggctggacg tgcgcgcgat cgccgcggac gtccgccagc ggttgttgga
     1501 ttccttcggg gccgaaccgc tctccaccgg ccggtggccc gtggttttcg agccccgggt
     1561 gggggtggac ctctgggagc ttttgtccca ggcggtggcc ggcgacgcgg tgcaaaaagg
     1621 gcggtctttt ttgggaacga aactgggaaa acccgtgggt tcgagccgcg tgacgatccg
     1681 ggacgacggg cgccttcccg gcgggctggc ctccggcccc g
//
LOCUS       DAOQLS010000503         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_51589_length_1720_cov_2.870871, whole genome shotgun sequence.
ACCESSION   DAOQLS010000503 DAOQLS010000000
VERSION     DAOQLS010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51589_length_1720_cov_2.870871"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1720
                     /locus_tag="PK362_08905"
     CDS             <1..>1720
                     /locus_tag="PK362_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74983.1"
                     /translation="YVLDRDGNLFRDGALVRNVNPDAASAESLAGKISAQSKLSIDAV
                     RDYLSRGPSAGAAAEATFIEGHLAGAMHDFVRSALVNSGVKLNGDMSAVASAVVEEID
                     AYRDDQGLSFAAAVARFGAENLKQGRPEVARALAADLAVSLPTEADRAPFRHLVKMLD
                     ARSQLAPRLEGLKKAYDIRGLTGGKDRLMTPEFVYAAAYTDGLALAKEFPKDTPAVMI
                     TGDQRWSTPALRQAAIDGYTAAGAHVYFSNERVSTPAANVVPRLGAFSVEGRPVLFHR
                     VLQISASHDPWLGDASKNGMKMNGRKDFHLRSMEGPQVAAMLEGMFSAEAGRAPDPAK
                     RGRATEIHLLPAYTEFVARRFVGAVEQYGLERADLEKLVFVTDGGQGVMDDVTPGVVD
                     GINRALRERFNDPAFSLAFDSVHPASVRGPDGTVEHPADPVEAGHFADHLKTEMDRVR
                     AAHPGKIVVFAVLDGDGDRSGLMGADGKVLSSTQQAFLYFNRLLADRPADRERLRLDI
                     RMGQEILELVGQVTPANQSMIDPGWSKQRRVLLQANAPGGIEGSSHTFVRISDDANTE
                     PMDDGLV"
BASE COUNT          295 a          563 c          571 g          291 t
ORIGIN      
        1 agtatgtcct ggaccgggac ggaaacctgt ttcgcgacgg ggcgttggtg cgcaacgtca
       61 accccgacgc ggcctcggcg gaaagtttgg ccggaaagat ctccgcccaa tccaagttgt
      121 ccatcgacgc cgttcgggac tatttgtcgc gggggccgtc cgcgggcgcc gccgccgagg
      181 ccactttcat cgagggtcac ctggccgggg cgatgcacga cttcgtccga agcgccttgg
      241 ttaattccgg cgttaaactg aacggcgaca tgagcgccgt ggccagcgcg gtcgttgaag
      301 aaatcgacgc ctaccgggac gaccagggtt tgtctttcgc cgccgccgtg gcccggttcg
      361 gcgcggaaaa ccttaaacaa ggccggcccg aagtggcccg ggccctcgcc gccgatttgg
      421 cggtttccct tcccaccgag gccgaccggg ccccgttccg tcacttggtt aaaatgctcg
      481 acgcccggtc ccagctggcg ccgcgattgg aagggctgaa aaaggcctac gacatccgcg
      541 gattgaccgg cggcaaggac cgcctgatga cgcccgagtt cgtctacgcc gccgcctaca
      601 ccgacggctt ggccctggcc aaagagtttc cgaaggacac gccggcggtg atgatcaccg
      661 gcgaccaacg ctggagcacc ccggcccttc gccaggcggc catcgacggc tacaccgccg
      721 cgggcgccca cgtgtatttc agcaacgaac gcgtttcgac gccggcggcc aacgtggtgc
      781 cgcgcctggg cgccttttcg gtggagggcc gcccggtcct gttccaccgc gtgctccaaa
      841 tttcggccag ccacgacccc tggctgggcg acgcttccaa aaacgggatg aaaatgaacg
      901 gacgcaagga tttccacctt cggtccatgg aaggtcccca ggtggccgcc atgttggagg
      961 gaatgttttc ggccgaagcg ggccgggcgc cggaccccgc caagaggggg cgcgcgacgg
     1021 agattcacct cctccccgcc tacaccgaat tcgtggcccg gcggttcgtg ggcgcggtgg
     1081 agcaatacgg gctggagcgg gccgatctcg aaaaattggt ttttgtgacg gacggcgggc
     1141 agggcgtcat ggacgacgtg accccgggcg tcgtggacgg catcaaccgg gctctgcggg
     1201 agcgttttaa cgaccccgcc ttctccctgg ccttcgacag cgtccacccg gcgtcggtgc
     1261 gcggccccga tgggacagtg gaacacccgg ccgatcccgt ggaagcgggg catttcgccg
     1321 atcatttgaa gacggaaatg gaccgcgtgc gcgcggcgca ccccgggaaa atcgtggtgt
     1381 tcgccgtttt ggacggcgac ggcgaccggt cgggcctcat gggcgccgac gggaaggtgt
     1441 tgtcgtcgac ccagcaggct ttcctctatt tcaaccgcct tctggccgat cgtcccgcgg
     1501 accgggaacg tctgcggctc gacattcgca tgggccagga aatcttggaa ttggtgggtc
     1561 aagtgacccc ggccaaccaa agcatgatcg atcccggctg gtcgaaacag cgccgcgtgc
     1621 tgctgcaggc caacgccccg ggcggcattg aagggtcctc ccacaccttt gtccggattt
     1681 cggacgatgc caacacggag cccatggacg acggcctggt
//
LOCUS       DAOQLS010000504         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_51810_length_1716_cov_3.418423, whole genome shotgun sequence.
ACCESSION   DAOQLS010000504 DAOQLS010000000
VERSION     DAOQLS010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51810_length_1716_cov_3.418423"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK362_08910"
     CDS             <1..250
                     /locus_tag="PK362_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74984.1"
                     /translation="LPPDVLDALNRGQFLEAIKRLRTVQNLDLKTAKDSMDAYLKNHP
                     ELMTRIKLQQEEFLQQVKKWLWIAGAVAAGLWLWRYYR"
     gene            401..1270
                     /locus_tag="PK362_08915"
     CDS             401..1270
                     /locus_tag="PK362_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74985.1"
                     /translation="MSKKESSDLEELIRSSPTIVVARHRSDMAQDIPIENESSFSHIK
                     TLLRQFDRVEVLRDESGSVPPRFTVGDPNLATRMRLQQMGKENAGGLFPVYDGAPPQD
                     PDGVVLFLRPSRENPGANFVFTVEGAVEHRSRKNEIEHLVEQCPYDPDAIPHFSKKPG
                     PAKTAAPVRHVEDPPVPCHLQAEVRYFAKEATELVIDLAVSMDGHPLFRRDVRASGDT
                     SDPRFMPYSFPRGTHRLLVVSRGMNLRFEKEINLTKPTWVVISYNHYDSDSTQRYKEG
                     LSIEVYDHEIFPR"
     gene            1325..1663
                     /locus_tag="PK362_08920"
     CDS             1325..1663
                     /locus_tag="PK362_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74986.1"
                     /translation="MTAPMSRGRQRLGGLGLFLLGAAMTAWEWKTALIDGYYHPKAAV
                     LFPCFAWLGLGLLLFPGYREERLARGESLEGREGLRLLTPRWWAILATGLALGFGHWW
                     FMARMPSNLP"
BASE COUNT          347 a          564 c          484 g          321 t
ORIGIN      
        1 ccttcccccc gatgtcctgg acgctttgaa ccgcggccaa tttttggaag ccatcaagcg
       61 cctccgcacg gtccaaaatc tcgacttaaa aaccgccaag gactccatgg acgcctacct
      121 caagaatcac cccgaattaa tgacgcgaat caaactccag caggaggaat tcctccagca
      181 ggttaaaaaa tggctttgga tcgcgggggc cgtggcggcg ggcctttggc tctggcgtta
      241 ttaccggtag ggatcgcccc gcggggtcgt ttcgcccgtt gacagggaag gcatccggac
      301 ttataaagag gggagccttt tcgcgcctcc gttccctccc gcggcccgtg gaatggaagg
      361 tccgcagcga tttcgtcaat tcacgaggcc ccagggtgcc atgtccaaaa aagaatcttc
      421 cgaccttgaa gaactgattc gatcgtcgcc caccatcgtc gtggcccgcc accgaagcga
      481 catggcccag gacatcccca tcgaaaacga gtcgtccttt tcccatataa aaaccctgtt
      541 gcggcaattc gaccgggtcg aagtccttcg cgacgaatcc ggaagcgtcc ccccgcggtt
      601 caccgtgggc gaccccaacc tggccacgcg catgcgcctt caacaaatgg gaaaggaaaa
      661 cgccgggggg ctctttcccg tctacgacgg cgccccgccc caagaccccg acggggtggt
      721 tctgttcctg cggccctccc gggaaaatcc gggggccaat tttgttttca ccgtggaggg
      781 ggccgtcgaa caccggtccc ggaaaaacga gatcgaacac ctggtcgagc aatgccccta
      841 cgacccggac gccattcccc atttcagtaa aaagccgggc ccggcgaaaa cggcggcgcc
      901 cgtccgccac gtggaagatc cccccgtgcc ctgccacctc caggcggagg tgcgttattt
      961 cgccaaagaa gccacggaac tggtgatcga cctggccgtc tccatggacg gacacccgct
     1021 gttccgccgg gacgttcggg cgtccggcga cacctccgac ccgcggttca tgccctattc
     1081 gttcccgcgg ggaacccatc ggctgctcgt cgtgtcccgg ggcatgaacc tgcgtttcga
     1141 gaaggaaatc aatctcacca aaccgacttg ggtggtcatt tcctacaacc attacgacag
     1201 cgactcgacc caacggtaca aagaaggtct ttcaatcgag gtttacgacc acgagatttt
     1261 cccccgctga ccgcccccgg agaaccggcg cggcgaccca ttcgctgaaa cgaccccttt
     1321 tcggatgacg gccccgatgt cccgtggacg ccaacggctg ggcggcctgg gtcttttttt
     1381 gctcggcgcg gccatgaccg cctgggaatg gaaaacagcc ctcatcgacg gctattacca
     1441 ccccaaggcg gccgttcttt ttccctgttt cgcctggctg gggctggggc ttctcctatt
     1501 cccgggctac cgggaggaac gcttggcccg gggcgaatcc ctcgaagggc gcgaagggct
     1561 tcgcctcctg acgccgcgct ggtgggcgat cctggcgacg ggtttggcgc tggggttcgg
     1621 ccattggtgg ttcatggccc gaatgccgtc gaacctccct tgataccggc ggttcatcaa
     1681 cgacgaaaaa aggaacatcc atgaaaacac aaaaga
//
LOCUS       DAOQLS010000505         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_52156_length_1710_cov_3.818127, whole genome shotgun sequence.
ACCESSION   DAOQLS010000505 DAOQLS010000000
VERSION     DAOQLS010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52156_length_1710_cov_3.818127"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PK362_08925"
     CDS             complement(<1..879)
                     /locus_tag="PK362_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC74987.1"
                     /translation="MTRLKVVHVVTQLELGGAQQNTLHTVRRLNRAEFEPFLLCGPGG
                     ILDAEAGRESVPVTFVSALQRAVHPFKDPAAFFQLRRAFRSLRPDIVHTHSSKAGILG
                     RWAAWSAGVPVVLHTFHGFGFTPGQSAPVRAAYVAAERWTARISTALVAVSRANRDEA
                     LARGIGRPDQFHLIRSGVPLGPYRSLAPRREPPAGIDLRPEEKLVLTIGPFKPQKNLK
                     DFVRAAAAVLQRRSDVKFLVVGDGEGRLRLEEDLRRRGVADRVILAGWRRDVPALLAR
                     TDVFCMTSLWEGLPRSL"
     gene            complement(876..>1710)
                     /locus_tag="PK362_08930"
     CDS             complement(876..>1710)
                     /locus_tag="PK362_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74988.1"
                     /translation="LYAHNLVLEMAAERGLLGLGAFLAFAVLLIRRGGGPSPRRRALG
                     LSAAAFGTFNLVNFGFSFPALYWLSFAGAGLYWAEAKSTEAPPRWTTHRRLGLAGVVL
                     FAGAGFASYALFRSGQCVERARIEFESGRWPAAREWVDRGLRWNRWNPELYDLGAALR
                     LVARDVDGAARDLDRCVGLAPTSAGFRRDAAELALRRSRPEEALAHYDAAVRFLPLSP
                     VPWVRRGDLLRSLGRLADAEASYRSALRALADPRVMAGAPDTRAALTTQVNAKLAEVL
                     P"
BASE COUNT          351 a          608 c          545 g          206 t
ORIGIN      
        1 cagggaccgg gggagacctt cccagaggga cgtcatgcaa aacacgtcgg tacgcgccag
       61 gagcgccggg acgtcccgcc gccacccggc gagaatcacc cggtcggcca ccccccgccg
      121 gcgaagatcc tcctccaacc ggaggcggcc ctccccgtcg cccaccacca gaaatttcac
      181 gtcggagcgc ctctgaagga cggcggcggc cgcgcgcacg aaatccttta agtttttttg
      241 gggtttgaac ggtccgatgg tcaggaccaa tttttcctcc ggacgaaggt caatcccggc
      301 cggaggttcc cggcgcgggg ccaaggaccg gtagggcccc aggggcacac cgcttcgaat
      361 caaatggaat tggtccggcc gcccgatgcc gcgggccagg gcttcgtcgc gattggcccg
      421 ggacacggcg accagcgcgg tggaaatccg ggccgtccac cgctcggccg ccacgtaagc
      481 ggcgcgcacc ggcgccgatt ggccgggcgt gaatccgaac ccgtgaaagg tgtgaaggac
      541 gaccggaacg cccgccgacc acgcggccca acgacccaga atgccggctt tggaggaatg
      601 ggtgtggaca atgtccggac gaagggaacg gaacgcccgg cgcaattgaa aaaaagccgc
      661 cgggtccttg aacgggtgaa cggcccgctg caaggccgaa acaaacgtca ccggcacgga
      721 ctcccgtccg gcctccgcgt ccaaaatgcc ccccggaccg caaagcagga acggttcaaa
      781 ttccgcccgg ttcaaccgac gaacggtgtg cagcgtgttc tgctgggccc cgcccaattc
      841 gagctgggtg acgacgtgga ccactttcaa tcgagtcacg gaaggacctc cgctaatttg
      901 gcgttgacct gcgtcgtcaa cgccgcccgc gtgtccggag cgcccgccat cacccgggga
      961 tccgccaagg cccggagggc cgagcggtag gacgcctcgg cgtccgccaa ccggcccaag
     1021 gaccgcaaca ggtccccccg tcgaacccag ggcaccgggc tcaggggaag aaaacgaacc
     1081 gcggcgtcgt agtgggccag cgcctcctcg ggacggctcc gccgcagggc caactcggcg
     1141 gcgtcccgtc gaaacccggc gctggttggg gccaacccca cgcaacggtc caggtctcgg
     1201 gcggcgccgt cgacatcgcg cgcaaccaat cgcagggccg ccccgagatc gtaaagctcg
     1261 gggttccacc ggttccatcg aagaccgcgg tcgacccatt cccgggccgc cggccatcga
     1321 cccgattcaa attcgatccg cgcccgctcc acgcactgcc ccgatcgaaa aagggcgtag
     1381 gaagcgaaac ccgccccggc gaacaacacc accccggcca accccagacg ccggtgggtc
     1441 gtccaccggg gcggggcctc cgtcgatttg gcctccgccc aatagaggcc ggccccggcg
     1501 aaactgagcc agtaaagggc cgggaaagag aatccgaaat tgacaagatt aaacgtcccg
     1561 aaagccgcgg ccgaaagacc gagagcccgc cgccggggcg agggaccgcc ccctcggcga
     1621 atcagcagga cggcgaaagc caaaaaagcg ccgagcccca gaagcccccg ttccgccgcc
     1681 atttccaaaa ccagattgtg ggcgtaaagc
//
LOCUS       DAOQLS010000506         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_52457_length_1705_cov_4.312121, whole genome shotgun sequence.
ACCESSION   DAOQLS010000506 DAOQLS010000000
VERSION     DAOQLS010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52457_length_1705_cov_4.312121"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1540)
                     /gene="sufB"
                     /locus_tag="PK362_08935"
     CDS             complement(125..1540)
                     /gene="sufB"
                     /locus_tag="PK362_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNC74989.1"
                     /translation="MSSKTDLKGLGKEYEYGFHDSTAGYAFQSGRGLTRDIVERISDM
                     KKEPAWMREFRLSSLDVFNKKPMPTWGNTQALGDIQFDNIHYYMKPTDKQGRTWEEVP
                     EGIKNTFDRLGIPEAERKFLAGVTAQYESEVVYHSIRKDLEKLGVLFLSMDDGLKLHP
                     EIVKKYFGTVIPPADNKFAALNSAVWSGGSFIYVPKGVNVPIPLQAYFRINSENMGQF
                     ERTLIIADEGSFVHYIEGCTAPTYSSDSLHSAVVELIALPGAKIRYTTIQNWSKNVYN
                     LVTKRAVAHANATVEWIDGNLGSKLTMKYPAVYLVGEGARGEILSVAFSGKDQHQDAG
                     AKIVHAAPHTTSVVTSKSISKDGGRSSYRGLIKVHKGAVGCKSNVRCDALIMDPASRS
                     DTYPTMEIDEEDTSIGHEASVSKIGEEQLFYLQSRGLSEAEATLMIVNGFFEPFVKEL
                     PMEYAVELNRLIELEMEGSVG"
     gene            complement(1551..>1705)
                     /gene="sufC"
                     /locus_tag="PK362_08940"
     CDS             complement(1551..>1705)
                     /gene="sufC"
                     /locus_tag="PK362_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342396.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNC74990.1"
                     /translation="QRLLDYVKPGFVHVLAGGRIVRSGGPELALELEKKGYEGYVPAP
                     VGAGAA"
BASE COUNT          272 a          501 c          587 g          345 t
ORIGIN      
        1 caggtcggtg cggcgccagg cttcgtcctc ggttttgggc caggggaggg cggcgaagag
       61 cgtcgccgcg ctctcgcgca gggcggtcaa gcccgccgac gtgggttcca gggcggcgtt
      121 caccctaacc caccgagcct tccatttcga gttcgatcag acggttgagc tcgacggcgt
      181 actccatggg gagttccttg acgaagggct cgaagaaacc gttcacgatc atgagcgtcg
      241 cctcggcctc ggagagaccg cgggactgca gatagaaaag ctgttcctcg ccgattttgg
      301 acaccgaggc ctcgtgcccg atggaggtgt cttcctcgtc gatctccatg gtggggtagg
      361 tgtcggaccg ggaggccggg tccatgatca aggcgtcgca ccggacgttg gacttgcacc
      421 cgaccgcgcc cttgtggact ttgatcaggc cgcggtagct ggaacggccg ccgtccttgg
      481 aaatggactt ggaggtcacg accgaggtcg tgtggggcgc cgcgtggacg atcttggcgc
      541 cggcgtcctg gtgctggtct ttgcccgaga aggccacgga gaggatttcc ccccgggcgc
      601 cctcgcccac caggtagacg gcggggtatt tcatggtcag cttggagccc aggttgccgt
      661 cgatccactc cacggtggcg ttcgcgtggg ccacggcccg cttggtcacc aggttgtaga
      721 cgtttttgga ccagttctgg atggtggtgt agcggatctt ggcgcccggg agcgcgatca
      781 gctcgaccac ggcggagtgg agcgagtccg aggaataggt cggcgcggtg cagccctcga
      841 tgtagtgcac gaaggagccc tcgtcggcga tgatgagcgt gcgctcgaac tggcccatgt
      901 tctccgagtt gatgcggaag taggcctgga gcgggatggg cacgttcacg cccttgggga
      961 cgtagatgaa ggacccgccg gaccagacgg cggagttgag cgcggcgaat ttgttgtcgg
     1021 cgggcgggat gacggtgccg aaatattttt tgacgatctc cgggtggagc ttcaacccgt
     1081 cgtccatgct gaggaagagc acgcccagct tttccaaatc cttccggatg gagtggtaga
     1141 cgacttcgga ctcgtactgg gcggtaaccc cggccaaaaa cttgcgctcg gcctcgggga
     1201 tgcccaggcg gtcgaaggtg ttcttgatgc cctcgggcac ttcttcccag gtgcggccct
     1261 gtttgtccgt gggcttcatg taatagtgga tgttgtcgaa ctggatgtcg cccagggcct
     1321 gggtgttgcc ccaggtgggc atgggctttt tgttgaagac gtccagggag gacaggcgga
     1381 attcccgcat ccaggcgggt tccttcttca tgtcggagat gcgctcgacg atgtcccgcg
     1441 tcagcccccg ccccgactgg aaggcgtagc ccgccgtcga gtcgtggaag ccgtactcgt
     1501 actccttgcc cagccctttg agatcggttt tcgaggacat ggtcccccct ttaggccgcc
     1561 ccggcgccca cgggcgcggg gacgtatcct tcgtaaccct tcttttccag ttccagggcc
     1621 agctccggcc cgccggagcg gacgatgcgc ccgccggcca gaacgtgcac gaagccgggt
     1681 ttcacgtaat ccaaaagccg ttggt
//
LOCUS       DAOQLS010000507         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_52469_length_1705_cov_3.471515, whole genome shotgun sequence.
ACCESSION   DAOQLS010000507 DAOQLS010000000
VERSION     DAOQLS010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52469_length_1705_cov_3.471515"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..989
                     /locus_tag="PK362_08945"
     CDS             21..989
                     /locus_tag="PK362_08945"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNC74991.1"
                     /translation="MAKKKEENRLIAPLKLFSGNAHPALAKEIAGVLKTQVGQASVGR
                     FPDGEVEVKITENVRGADCFIIQPTSYPANDNLMELLLLVDALKRASARRITAVIPYF
                     GYGRQDRKAEPRVPISAKLVSKMIEAAGVNRVLTMDLHAGQIQGFFDIPVDHLYANPV
                     LVSYFQKKKIRTEDLVVVSPDAGGVERARAFAKRLESGLAIIDKRRLSPREAAVMHVV
                     GDVKGKIALIIDDLVDTGGTLVKAAQALKESGAIKIMAAAAHGVLAGPAIERLNASPV
                     EGLVITNSIPLSHKDAARIRVLSVAPLLAEAIARIHGERSISEMFV"
     gene            1020..>1705
                     /locus_tag="PK362_08950"
     CDS             1020..>1705
                     /locus_tag="PK362_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNC74992.1"
                     /translation="MKTIELGAERRELASKGERRQLRSAGRIPAVVYGGKKDAAPLSI
                     DGKSLFQVLKGHGTNVIMNLKVGGAGETVLLKDLQRDFVTHNIVHVDFQRVSMTEKLE
                     VNVPLHIVGEAPGVKLSGGILEHILRELRVRCLPADIPDGINIDVSALQINQGVKVKD
                     LVLPKGVESLIDGGSLVVNIVAPAEEEVATPATAAPTGAEPEVIAKGKKPEEGEEGAA
                     AAPAAKGGAAP"
BASE COUNT          347 a          534 c          547 g          277 t
ORIGIN      
        1 tcttaaaata aggacaaccc atggctaaaa agaaagaaga aaatcgattg atcgctccct
       61 taaaactttt ttcgggtaac gcccatccgg cgttggccaa agaaatcgcc ggcgtattaa
      121 aaacccaggt gggccaggcc agtgtggggc ggtttccgga cggcgaggtg gaagtgaaaa
      181 tcaccgagaa cgtgcgcggc gcggactgtt tcattatcca gcccaccagt tatccggcca
      241 acgacaattt gatggagctc ctgctcctgg tggacgcgtt gaagcgcgcc tccgcccgcc
      301 gcatcacggc ggtgatcccc tatttcggct acggccggca ggatcgcaag gccgagcccc
      361 gcgtgcccat ctcggccaag ctggtttcca agatgatcga ggccgcgggc gtgaaccgcg
      421 tgctgaccat ggacctgcac gccggccaga tccaggggtt cttcgacatc ccggtggatc
      481 atctctacgc gaacccggtg ctggtgtcct atttccagaa gaaaaagatc cgcaccgagg
      541 acctcgtggt ggtctcgccc gacgccggcg gcgtggagcg ggcccgggcc ttcgcgaagc
      601 ggctcgaatc cggactggcc atcatcgaca agcggcgcct ctccccccgg gaagcggcgg
      661 tcatgcacgt ggtgggcgac gtgaagggca agatcgccct catcatcgac gatttggtgg
      721 acaccggcgg cacgctggtc aaagcggccc aggccttgaa agagtccggg gcgatcaaaa
      781 tcatggcggc ggcggcccac ggggtgctgg cgggcccggc catcgagcgc ctgaacgcct
      841 cgcccgtgga gggattggtc atcaccaaca gtattccgct cagtcacaaa gacgcggcgc
      901 gcatccgggt gttgtcggtg gcgccgctgt tggcggaggc catcgcccgg attcacggcg
      961 agcggtccat cagcgaaatg ttcgtttgat ttttcaaaac caagagaggt attgagtcca
     1021 tgaagacgat tgaattgggc gccgaacggc gcgagttggc gagcaaaggc gagcggcggc
     1081 agctgcgctc cgccggacgg attccggccg tggtctacgg gggcaaaaaa gacgccgccc
     1141 ccctctcgat cgacggcaaa agcctgttcc aggtcttgaa gggccacgga acgaacgtga
     1201 tcatgaacct caaggtcggc ggcgcgggcg aaaccgtgct gttgaaggac cttcagcggg
     1261 acttcgtgac ccacaacatc gtgcacgtgg acttccagcg cgtctccatg acggagaaac
     1321 tcgaagtcaa cgtcccgctc cacatcgtgg gcgaagcccc gggcgtgaag ctctcgggcg
     1381 gtattttgga gcacatcctc cgggaactcc gcgtgcgctg tttgcccgcc gacattccgg
     1441 acgggatcaa catcgacgtc tccgccctcc agatcaacca gggcgtgaag gtgaaggacc
     1501 tcgttctccc caagggcgtc gagtccttga ttgacggcgg cagcctcgtc gtcaacatcg
     1561 tggcgcccgc cgaagaggaa gtcgccacgc ccgccacggc cgcgcccacc ggcgccgagc
     1621 cggaagtcat cgccaagggc aagaagcccg aggaaggcga agaaggcgcc gccgcggccc
     1681 ccgccgccaa gggcggagcc gcccc
//
LOCUS       DAOQLS010000508         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_52518_length_1704_cov_4.301395, whole genome shotgun sequence.
ACCESSION   DAOQLS010000508 DAOQLS010000000
VERSION     DAOQLS010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52518_length_1704_cov_4.301395"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="dnaA"
                     /locus_tag="PK362_08955"
     CDS             complement(<1..1027)
                     /gene="dnaA"
                     /locus_tag="PK362_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNC74993.1"
                     /translation="MERLKPQFKEEIYNLWIKPLRPTAWGDARLTVQVPNRYFADWVQ
                     KNAQAAIESSLRDMLGRDGSVALVTSETQDVEPDSMPESPAPAPVVLSRVENHVSDET
                     FNPKYTFDTFVVGPSNRFAQAAAAAVSKDPGKTYNPLFLYGGVGLGKTHILHAIAHGI
                     RANNPGARVLYVTSERFINEFIDALRFDRMKDFRAKYRNLDCLLIDDIQSLMSKENSQ
                     EEFFYTFNSLYDSRKQIIISSDRTPNETKVSARLISRFEWGVVADIQPPDLETRIAIL
                     RKKAASENIFVPDDVILYIAGQIRSNIRELEGSLIRTVAFSSLTGTPLTIESARETLK
                     DIVAASAA"
     gene            complement(1476..>1704)
                     /locus_tag="PK362_08960"
     CDS             complement(1476..>1704)
                     /locus_tag="PK362_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74994.1"
                     /translation="DVEKLLGVLQRLVDGGNTVLVIEHNLDVVKTADWILDLGPEGGA
                     GGGRLVAEGAPETVAAAADSHTGRHLRPLLK"
BASE COUNT          371 a          454 c          511 g          368 t
ORIGIN      
        1 tggccgccga ggccgccacg atgtccttca gcgtttcccg ggccgattcg atggtcagcg
       61 gggttccggt cagggagctg aaggcgacgg tccgaatcag actgccttcc aattcgcgga
      121 tgttggagcg aatctggccg gcgatgtaga gaatcacgtc gtccgggacg aaaatgtttt
      181 cggaggcggc ctttttgcgc agtatcgcga tgcgggtttc caagtccggc ggctggatgt
      241 cggccaccac gccccactcg aaccgggaaa tcaaccgggc cgacactttg gtctcgttcg
      301 gggtgcggtc cgaggaaatg atgatttgct tgcgggaatc gtagagggag ttgaaggtgt
      361 agaagaactc ctcctgggaa ttctccttgc tcatgagcga ttggatgtcg tcgatcagca
      421 aacagtccag attgcggtat ttggcccgga aatccttcat gcggtcgaac cgcagggcat
      481 cgatgaattc gttgataaat cgctcgctgg tcacgtaaag gacccgggcg ccggggttgt
      541 tggcccggat gccgtgggcg atggcgtgaa gaatatgggt tttcccaagg cccaccccgc
      601 cgtagaggaa aagagggttg taggttttgc ccgggtcctt ggaaaccgcg gcggcggccg
      661 cctgggcaaa ccggttggaa gggcccacga caaatgtgtc aaaggtgtac ttgggattga
      721 aagtttcgtc cgacacgtgg ttttcgaccc gggacaaaac cacgggcgcc ggggccggcg
      781 attcgggcat cgaatccggc tccacgtcct gggtttcgga agtcaccagg gcaacggaac
      841 cgtcccggcc cagcatgtcg cggagggacg attcgatggc cgcttgggcg ttcttttgaa
      901 cccaatcggc aaaataccga ttgggcactt gaacggttaa gcgggcgtcg ccccaggccg
      961 ttggccggag gggtttaatc cacaaattgt agatttcttc tttgaattgg ggctttaaac
     1021 gctccaaaac ccccgtccaa atagactcgg cagtttcact caaaagcaag gaaaatctcc
     1081 acttatccac agacttatac acacggtctg ataataactg tggattatct taataactta
     1141 tattttgtta aatagtgcta tcttttgccg cggcgtggca acggcaagcc caggtccgaa
     1201 tcgaagtcgc tcgtgatttt tgggtatgga aaagggctgt gaataactga agcgcgccta
     1261 tcttaatgaa gcacgccaac gttgtcaagc gggcgaaaaa aaatttacaa aacaaaatgt
     1321 ccgacggaaa aatcgccggc cacggccaaa agttctcgtt caacgacgga aaaatatttt
     1381 ttttgagcgg gcgcgcgcgg gcttgacaag ccgcggcggt acgacttcat cgtcggaaaa
     1441 acaaatattg ttttcgcgtt cgggccggcg ccgcgttatt tcaacaacgg tcgaagatgg
     1501 cggccggtgt gggagtcggc cgcggcggcc acggtttcgg gcgcgccttc ggccaccaac
     1561 cgtccgccgc ccgctccgcc ttcgggcccc agatccaaaa tccagtcggc cgttttgacc
     1621 acgtccagat tgtgttcgat gaccaggacg gtgttgccgc cgtccaccag gcgctgaagc
     1681 acgcccaaca gtttctccac gtcc
//
LOCUS       DAOQLS010000509         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_52634_length_1702_cov_3.796600, whole genome shotgun sequence.
ACCESSION   DAOQLS010000509 DAOQLS010000000
VERSION     DAOQLS010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52634_length_1702_cov_3.796600"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1702
                     /locus_tag="PK362_08965"
     CDS             <1..>1702
                     /locus_tag="PK362_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74995.1"
                     /translation="LQVLLESRGPKPENVTKALRQVPEKDRPAENVLPPAPPKDTQDL
                     WQFPSEPRPDFSPYENTYFVPNTLQMRECNECFQRGETGCKQCFGKGEEACQNCLGSG
                     TQSCLYCKGLEKVTCIRCGGEGRMTAGGVQGVGRAARCDACNGTGKFPCTHCKEGKVP
                     CPACNGGGKGPCKKCNGQGKTACPTCLGDKKIVVGLAFQADFKPFQVAVTGLAVPGPR
                     EAVELAAPTVQPVGEIAVSADEPFEKQVQNLDAGTHGRATLQQLAEKLRSRLSPNTRA
                     VKYRLEVAEGNVIRVSGYCAGQEFSFWMAAGRPEIIAEKDPLASLSNSVAVAAEEALS
                     AGDWKKAVDLARETLSYSPGHLTALTILSDWRRKVFRECLVGVVASAVLAAVFFSGLI
                     LHADKGLHKTGALFQVSALQFVVALVAGVVAFPFLLRLYPIKVRWPSLAGALAAAHLA
                     YWIAAHWVLDWNPVRTADQAALNREMEAHFKFGVAKVFYEPDLLYLQGLYKTYKDSEA
                     DLSNLQSNLDKQLELKKAHEHDQKMFNAKVAEVAASGLSAVKKKERIEELKAYYKLLN
                     I"
BASE COUNT          341 a          517 c          534 g          310 t
ORIGIN      
        1 cttcaggtct tgctggagtc ccgggggccc aagcccgaaa acgtcaccaa agcccttcgc
       61 caggtgccgg aaaaggaccg tcccgcggaa aacgttctgc ccccggcgcc ccccaaagac
      121 acccaggatc tctggcaatt tccctcggag ccgcggccgg atttctcgcc gtacgaaaac
      181 acctatttcg tccccaacac gcttcaaatg cgcgaatgca acgaatgctt ccagcgcggc
      241 gaaacggggt gcaagcaatg tttcggcaag ggggaggaag cctgccaaaa ctgtcttggg
      301 tccggcacgc agtcctgttt gtattgcaag gggttggaga aagtgacgtg catccgttgc
      361 gggggcgagg gccggatgac cgcgggcggc gttcagggcg ttggccgcgc ggcccggtgc
      421 gacgcgtgca acggcacggg aaaattcccc tgcacccatt gcaaagaggg caaggtgccc
      481 tgcccggcct gcaacggcgg ggggaaaggt ccctgtaaaa aatgcaacgg ccaagggaag
      541 acggcctgcc ccacctgcct cggcgacaag aagatcgtcg tgggtttggc ttttcaagcg
      601 gatttcaagc cgttccaggt cgccgtgacc ggtctggccg ttccgggtcc ccgggaggcg
      661 gtcgaactcg cggccccgac ggtccagccg gtgggcgaaa tcgccgtctc ggccgacgaa
      721 ccttttgaaa agcaagtcca aaatctggac gccggaaccc acggccgggc cacgctgcaa
      781 cagctggccg agaaactgcg gtcccgtctg tcccccaaca cccgcgccgt caaataccgg
      841 ttggaggtgg ccgaaggcaa cgtcattcgg gtcagcgggt attgcgccgg gcaggaattt
      901 tcgttctgga tggcggcggg ccgcccggag atcatcgcgg aaaaggaccc cctggcctcc
      961 ctcagcaatt cggtcgccgt ggcggccgag gaggcgctca gcgccggcga ctggaaaaaa
     1021 gccgtggacc tcgcccggga aacgttgtcc tactccccgg gacacctgac cgctttaacg
     1081 atcttgtcgg attggcggag aaaagtgttc cgggagtgcc tcgttggggt ggtcgcttcc
     1141 gcggtcctcg ccgccgtttt cttttcgggg ctgatcctcc acgccgataa gggattgcac
     1201 aaaaccggcg ccctgttcca ggtctcggcc ctgcaattcg tcgtggccct ggtcgcgggg
     1261 gtcgttgcct ttcccttcct cctgcgtttg tatccgatca aagttcgctg gccctctttg
     1321 gcgggggccc tggcggcggc gcacctggcc tactggatcg ccgcccactg ggtgttggat
     1381 tggaaccccg ttcggaccgc ggaccaagcg gccctcaatc gcgagatgga ggcccacttt
     1441 aaattcgggg tggccaaggt tttctacgaa cccgacctgc tttatcttca ggggttgtac
     1501 aaaacctaca aggacagcga ggcggacctg tcgaatttgc agtcgaattt ggacaaacag
     1561 ctggagttga aaaaagccca cgagcacgac cagaaaatgt tcaacgccaa ggtcgcggaa
     1621 gtcgccgcgt cggggctgtc ggcggtcaaa aaaaaggaac gaatcgagga attaaaagct
     1681 tactataaac tcttgaacat cg
//
LOCUS       DAOQLS010000510         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_52661_length_1702_cov_2.629630, whole genome shotgun sequence.
ACCESSION   DAOQLS010000510 DAOQLS010000000
VERSION     DAOQLS010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52661_length_1702_cov_2.629630"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1702
                     /locus_tag="PK362_08970"
     CDS             <1..>1702
                     /locus_tag="PK362_08970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC74996.1"
                     /translation="LLGAVLLVQAAGVLRSNSSTYDEPGVIGAGVRYWDAGRTDMALR
                     DYPPLAKYLAGAAARIPGPERTARESFVAADRYYAYGFEFLYRNSVEPERLLTAARLP
                     SLALLLTVMVVAYLWAGRWYGPWGGALAAAAVVFEPNLLAHGCLATTDMPLTAFLFLA
                     FYFLVRHGEGGRWGDLVAAGLAAGAAIAVKTPGLLFYAVAPFVLAAAGAPPRWNRKDL
                     GRWAARGAILAAAAVLVLFVVYRGHPWDFYPRLLRASFGFGFDGAAKNYLAGEIRHGR
                     FWTYYLLAFAVKTPLPFIGLAGLAAVYGRSRRRALLLGAPIVALVLLFTFAQKQNGLR
                     YLLPAYPLLAVAIGGVAAAGPRARRIAAAGVLWMAIGTFRAMPYPLAYFNEAVGGPAR
                     GHRWLVDCNLDWGQDFPAIRALWEREGKPELLLATAGLADRAHYFGPQQDVTSDDNTP
                     PWLYYQHVNSTAPTKEWLVVAASKLHGWGVSGDPFAWLRNRAPIAQPGHSSFVFDVTR
                     DPESQFQLGLWYLNGTLYAPALRQFRRAGALDPGNPFPPLAEGDVLTLTGEAAAARRA
                     Y"
BASE COUNT          201 a          560 c          605 g          336 t
ORIGIN      
        1 cctgttgggc gccgtgttgt tggttcaggc cgccggcgtt ctccgctcga attcgtcgac
       61 ctacgacgaa cccggcgtga tcggggccgg ggtccgttac tgggacgcgg gccggacgga
      121 catggccctt cgggattacc cgcccttggc caaatatctc gccggggcgg cggcccggat
      181 tccgggcccc gaacgcacgg cccgggaatc cttcgtggcg gcggaccgtt actacgccta
      241 cgggtttgaa ttcctctatc gaaattccgt cgaacccgaa cgcctgctga ccgcggcgcg
      301 cctcccgtcc ctggccctgc ttttgacggt gatggtggtg gcgtatttgt gggcggggcg
      361 ctggtacggg ccctggggcg gcgccttggc cgcggcggcg gtcgtttttg aacccaacct
      421 tctggcccac gggtgcctgg cgacgacgga catgcccctg acggcgtttt tgtttttggc
      481 cttttatttc ctcgtccggc acggcgaagg ggggcggtgg ggggatttgg tcgccgcggg
      541 tctggccgcg ggcgcggcca tcgcggtcaa gacgcccggg ctcctgttct acgccgtggc
      601 gcccttcgtc ctggcggcgg ccggggcgcc gccccgctgg aaccggaagg acctggggcg
      661 ctgggccgcc cgcggcgcga tcctggcggc cgcggcggtt ctggtgctct tcgtcgttta
      721 tcgggggcat ccctgggatt tctatccccg gcttcttcgg gccagcttcg ggttcggctt
      781 cgacggggcg gcgaaaaact atttggcggg ggagattcgc cacgggcggt tttggacgta
      841 ttacctcctg gccttcgccg tgaaaacccc cttgccgttc atcggactgg cggggttggc
      901 ggccgtttac ggacggtccc ggcgtcgggc gttgttgctg ggcgcgccga tcgtggccct
      961 ggtcctcctg ttcacgttcg cgcagaaaca aaacgggctg cgctatctcc tgccggctta
     1021 cccgcttctc gcggtggcca tcggcggggt ggcggccgcg ggaccccggg cccggcggat
     1081 cgcggccgcc ggcgttctct ggatggcgat cggcaccttc cgcgcgatgc cgtacccgct
     1141 cgcctatttc aacgaagccg tcggcggtcc cgcccggggc caccgctggt tggtggactg
     1201 caatttggat tggggtcagg acttccccgc gattcgcgcg ctctgggagc gggaggggaa
     1261 gcccgaattg ttgctggcca cggcgggcct ggccgaccgg gcccactatt tcggccccca
     1321 gcaggacgtg acctcggacg acaacacccc gccctggttg tattaccaac acgtcaattc
     1381 gacggctccg acgaaggaat ggctggtcgt ggccgcgagc aaacttcacg gctggggcgt
     1441 gtcgggggac cccttcgcct ggttgcgaaa ccgcgcgccg atcgcccagc cgggacacag
     1501 ctccttcgtg ttcgacgtca cccgggaccc ggagagccaa tttcaattgg gtctttggta
     1561 tttgaacggg acgctgtacg cgccggcgct ccggcagttt cggcgggcgg gggccctcga
     1621 tccgggcaat ccctttcctc cgctggccga aggggatgtg ttgaccttga ccggcgaggc
     1681 ggcggccgcc cgccgggcct ac
//
LOCUS       DAOQLS010000511         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_52692_length_1701_cov_3.783111, whole genome shotgun sequence.
ACCESSION   DAOQLS010000511 DAOQLS010000000
VERSION     DAOQLS010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52692_length_1701_cov_3.783111"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK362_08975"
     CDS             complement(<1..363)
                     /locus_tag="PK362_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNC74997.1"
                     /translation="MNNVERPEIQVGDKKISFEVGGVARQAAGAALVRMGDTVVIVAA
                     ANGTKPREDRDFFPLTVDYRERTYAAGRIPGGFFKREGKARDNEILTSRLIDRSIRPL
                     FPEYLRLEVQITALALSHD"
     gene            complement(521..787)
                     /gene="rpsO"
                     /locus_tag="PK362_08980"
     CDS             complement(521..787)
                     /gene="rpsO"
                     /locus_tag="PK362_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNC74998.1"
                     /translation="MITKEKKHDLVSKFGSSPKDTGNPAAQIAILTERINGLMDHFQK
                     APKDHGSRRGLLMMVGHRRRLLSHLRESEPKRYVELLKELNLRK"
     gene            complement(837..1646)
                     /locus_tag="PK362_08985"
     CDS             complement(837..1646)
                     /locus_tag="PK362_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC74999.1"
                     /translation="MKRAVLRGTAAILLAAAGSARAGFSGPEDLGVFADAPPTSSTHF
                     RLHHEDPFVPAGILNDLEGLHAKLLLDLGVFSPWAYSERIDVYLYKNAESYAEHAHMP
                     AWSAAHIDFQNRKIYGYPSAELKRTLAHEMGHLFFMQYFVAQSATPPLWLNEGVAMLM
                     EWDYGGDGDSGQRDRFLMKNRPPALTDFFKTTYQGAGNDQGRAVSLWYAQAGSVTRFL
                     MRRFSKDQFVRFCAALREGKSVDEALPLAYGLAVSDAAALDRLWRESVNAE"
BASE COUNT          328 a          476 c          564 g          333 t
ORIGIN      
        1 gtcgtgggaa agggccaggg cggtgatttg gacttccaac cggaggtatt cggggaacaa
       61 cggccggatg gatcgatcga tcaggcggct ggtcaaaatt tcgttgtccc gggccttgcc
      121 ttcgcgctta aagaagccgc cgggaatgcg gcccgcggcg taggtgcgct cgcggtagtc
      181 gacggtcaac gggaaaaaat cgcggtcttc gcggggtttc gtgccgttgg cggcggcgac
      241 gatcaccacc gtgtcgccca tgcggaccag cgcggcgccg gcggcctggc gggccacccc
      301 gccgacttcg aaggaaattt tcttgtcgcc cacttggatt tcgggtcgtt caacgttgtt
      361 catgataaaa ggactccttt ggagtgtaaa aaaaacgcgg agccccttgg acggccggcg
      421 ggttttgcgg tgttcggaag ggatcggaac gccccacgat catggagcct tcgcatccct
      481 tgcgtgtgca aaacaaccgg cgcgccgaaa gggcgccgcg ctattttcga aggttaagtt
      541 ctttgagcag ttccacgtac cgcttcggtt cggattcccg caggtgcgac aggagtcgac
      601 gccggtgacc caccatcatc aaaagtccgc gccgagaacc gtggtccttg ggggcttttt
      661 gaaagtggtc catgaggccg ttgatgcgct cggtcaaaat cgcgatttgg gcggccgggt
      721 ttcccgtgtc tttgggagag gaaccgaatt tggatacgag gtcgtgtttc ttttcttttg
      781 taatcatcgg gaggatttta gcaaatgaat gaaaatcctg taaaggattt tcttgctcac
      841 tcggcgttga cggactcccg ccacagccgg tccagggcgg ccgcgtcgga caccgcgagg
      901 ccgtaggcga ggggcagggc ctcgtcgacg cttttaccct cccgcagggc cgcgcaaaaa
      961 cgcacaaact ggtccttgga aaaccggcgc atcaaaaagc gggtgacgct gccggcttgg
     1021 gcgtaccaaa gggagaccgc ccgcccctga tcgttgccgg cgccttggta cgtcgtctta
     1081 aaaaaatccg tcaaagcggg cgggcggttc ttcatcaaaa agcggtcccg ctggccggag
     1141 tcgccgtccc ccccgtagtc ccattccatc agcatggcca cgccctcgtt cagccacagc
     1201 ggcggcgtgg ccgattgggc gacgaaatat tgcataaaga acaggtggcc catttcgtgg
     1261 gccagagtcc gtttcagttc ggcggagggg tagccgtaaa tcttccggtt ttgaaaatcg
     1321 atgtgggcgg cggaccaggc gggcatgtgg gcgtgttcgg cgtagctctc ggcgttcttg
     1381 taaagataaa catcgatgcg ttctgaatag gcccagggag aaaaaacgcc caaatccagc
     1441 aagagcttgg cgtgcaaccc ctcgaggtcg ttgagaatcc cggcggggac gaaggggtct
     1501 tcgtgatgaa ggcgaaaatg ggtcgaagag gtcggggggg cgtcggcgaa aaccccgagg
     1561 tcctcggggc ccgaaaaccc ggcgcgggcg cttccggcgg cggccaggag gatcgcggcg
     1621 gttccccgaa gaacggcgcg cttcacggcg tttcccgccg ggacatttcc cggtcctgcc
     1681 ggcggcccag cgcgatctgg g
//
LOCUS       DAOQLS010000512         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_52930_length_1697_cov_3.985993, whole genome shotgun sequence.
ACCESSION   DAOQLS010000512 DAOQLS010000000
VERSION     DAOQLS010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52930_length_1697_cov_3.985993"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PK362_08990"
     CDS             complement(<1..1051)
                     /locus_tag="PK362_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNC75000.1"
                     /translation="MIRPGLERIRKVLSRLGHPEDAFAAVIVAGTNGKGSVAAMIESI
                     LRAAGHRTGLYTSPHLVDLRERVRFDGAPISAARWRRLEARVRRAGRGTGLSEFETQT
                     ALAFLAFAESGVDLAVLEVGLGGRWDATNAHPAPEATVVTSIGLDHREWLGPTLRHIY
                     REKREVGRPGTVMVQHLPRGFWKESDRWADDLGAPLWTLGREVRVRSTSGRMSVRGPG
                     FQFSNLAVPFDGPHQVQNAALAVAVSAALMARGWKIPAASLRRGIAGARWPGRFDVVD
                     RRGPVVLDGAHNADAARALARAWRTSSWGREKATLLFGCLKDKDAAAMAKHLAPVAKR
                     VFVTALPTPRSRTADE"
     gene            complement(1065..>1697)
                     /locus_tag="PK362_08995"
     CDS             complement(1065..>1697)
                     /locus_tag="PK362_08995"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HNC75001.1"
                     /translation="VDPLGFVDTASYAQRLKESKKKTGEREAILAGVAQMNGQPVALA
                     VLDFGFMGGSMGSVVGEKVTLAIERGLKDQLPVIVVSASGGARMQEGILSLFQMAKTS
                     AALARLEKARLPFISVVTDPTTGGVTASFAMLGDVIVAEPKALVAFAGPRVIEQTIRQ
                     TLPQGFQQSEFLLKHGMVDVVVDRAHLKSRLAELAAFFMAARKAATAAAV"
BASE COUNT          297 a          574 c          570 g          256 t
ORIGIN      
        1 actcgtccgc cgtgcgggac cgcggcgtgg gcaacgcggt cacgaacacg cgcttggcca
       61 ccggggccaa atgtttggcc atggcggcgg cgtccttgtc cttcaagcaa ccgaaaagga
      121 gcgtcgcttt ttcccggccc caggaagacg ttcgccaggc tcgggccagg gcccgggcgg
      181 cgtcggcgtt gtgggccccg tccaggacca cggggccccg gcggtccacc acgtcgaaac
      241 gaccgggcca acgggccccg gcgattcccc gacggagaga cgccgcgggg attttccacc
      301 cccgggccat caaagccgcc gacaccgcga cggccagggc ggcgttttga acttggtggg
      361 ggccgtcgaa tggaaccgcc agattcgaaa attggaaacc cggccctcgc accgacattc
      421 gcccggacgt cgaacgaacg cgaacctccc ggcccagggt ccagaggggg gcgccaaggt
      481 cgtcggccca acggtcgctc tctttccaaa aaccccgggg aagatgctgg accatcaccg
      541 tgcccgggcg gcccacctcc cgcttttccc ggtaaatgtg ccgcagggtc ggcccgagcc
      601 actcccggtg gtccaacccg atggaggtga ccaccgttgc ttccggcgcg gggtgggcgt
      661 tggtggcgtc ccaacggccg cccaggccca cctccaacac cgccaggtcc acgccggatt
      721 cggcgaaggc caggaaggcc agggccgttt gagtttcaaa ttcggaaagt cccgttcccc
      781 gaccggcccg acggacccgg gcttcaagcc gccgccatcg ggcggcggaa atcggagcgc
      841 cgtcgaagcg gacacgctcc cgaagatcca ccaaatgggg cgaggtgtaa agaccggttc
      901 gatgacccgc cgcccgaaga atggattcga tcattgccgc aacggaccct ttgccgttgg
      961 tcccggcaac gatgaccgcg gcgaaggcgt cctcggggtg gcccaagcgg gagaggacct
     1021 tccggatccg ctcgaggccg ggccgaatca agggcgggga agcgtcagac ggccgccgcc
     1081 gtggcggctt tgcgcgcggc catgaaaaag gccgccagct ccgccaggcg ggacttgaga
     1141 tgggcgcggt ccacgacgac gtccaccatg ccgtgcttga gcagaaattc cgactgctga
     1201 aacccctggg ggagggtttg gcggatggtt tgctcgatga cccggggacc ggcgaaagcc
     1261 accagggcct tgggttcggc cacgatcacg tcgcccagca tggcgaaact ggcggttacg
     1321 ccgccggtgg tggggtccgt cacgacggaa ataaacggca ggcgcgcctt ttcgaggcgg
     1381 gccagggccg cgctggtttt ggccatttgg aacagggaga ggatgccctc ctgcatgcgg
     1441 gccccgcccg aagccgagac gacgatgacc ggcagttggt ctttcaggcc gcgctcgatg
     1501 gcgagggtga ctttttcccc cacgacgctt cccatgcttc cgcccatgaa tccgaaatcc
     1561 agaacggcca gggccacggg ctgcccgttc atttgggcca cgccggccaa aatggcctcc
     1621 cgttcaccgg tcttcttttt gctttccttc aggcgctgag cgtacgacgc ggtgtccacg
     1681 aaccccaggg ggtccac
//
LOCUS       DAOQLS010000513         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_53036_length_1695_cov_5.423171, whole genome shotgun sequence.
ACCESSION   DAOQLS010000513 DAOQLS010000000
VERSION     DAOQLS010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53036_length_1695_cov_5.423171"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..435)
                     /locus_tag="PK362_09000"
     CDS             complement(79..435)
                     /locus_tag="PK362_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNC75002.1"
                     /translation="MESGHSFMGSVITLALIALVLVAFWKVFEKAGRPGWQAIVPFYN
                     LYVLCQIVGWPGWYLVFFLIPLVNMVMTAVLQFKVAAVFKKGVGFAIGLILLPIVFLP
                     ILGFGDDTYTAPAPKA"
     gene            complement(486..1595)
                     /locus_tag="PK362_09005"
     CDS             complement(486..1595)
                     /locus_tag="PK362_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HNC75003.1"
                     /translation="MALVPGTVTVNPEDADVSTRLGPVKLDIPILASAMDGVVDVEFA
                     VAMGRLGGLAVLNLDGVQTRYENPKPLFKKIAEATPEVATKLVQDIYSKEPVKDKLVA
                     QRVKEIKKGGVLCAVSSIPQNAERVGKIAADAGADLFIIQGTVTTARFKSSKGPVVDL
                     AKLCQKLKIPVIIGNVVTYSAALELMETGAAALLVGVGPGAACTSRGVLGLGVPQVTA
                     TVDCAAARDFYYKKSGRYVSIITDGGMSTGGDVCKAFASGADAVMVGSVFARAEEAPG
                     RGFHWGMATPHHNLPRGTRVRVGTVGTLKQILHGPASLDDGTQNIVGAIRTCMGTVGA
                     TNIKEFQLTEIIIAPEIKHEGKLFQQAQKVGMGKK"
BASE COUNT          319 a          538 c          533 g          305 t
ORIGIN      
        1 caaggggaga gggaaaaacc cacgcgccca gaaaaatgcg aggccgggcg cctggccggg
       61 gcgcaaaact cggcgacgtt acgccttcgg cgccggcgcc gtgtacgtgt cgtcgccgaa
      121 acccaaaatc ggcagaaaca cgatgggcaa caggatgagc ccgatggcga acccgacgcc
      181 cttcttaaac acggccgcca ctttgaactg gaggacggcg gtcatgacca tgttgaccag
      241 ggggatcagg aagaacacca gataccagcc cggccagccg acgatttggc agaggacgta
      301 gaggttgtag aaaggcacga tggcctgcca acccggacgg cccgcttttt caaagacctt
      361 ccagaacgcc accaacacca acgcgatcaa tgccaacgtg atgaccgaac ccatgaagct
      421 gtgacccgat tccatgcgtc ccccttgtgc ggagtgtggt tgtcccgggg cccgaaggcc
      481 gacggttatt tcttccccat ccctactttt tgcgcctgct ggaacaactt gccttcgtgt
      541 ttgatttccg gcgcgatgat aatttcggtg agctggaact ccttgatgtt ggtggcgccc
      601 accgtgccca tgcaggtgcg gatggccccc acgatgttct gcgtgccgtc gtccagggag
      661 gccggcccgt gcaggatctg cttgagcgtg cccaccgtgc ccacccgcac gcgggtgccg
      721 cggggcaggt tgtggtgcgg ggtcgccatg ccccagtgga agccccggcc gggggcttcc
      781 tcggcccggg caaaaacgga cccgaccatg accgcgtcgg cgcccgaggc gaaggccttg
      841 cagacgtcgc cgccggtgct catgccgccg tcggtgatga tggacacgta gcggccggac
      901 ttcttgtagt aaaagtcccg ggccgcggcg cagtccacgg tcgcggtcac ctggggaacg
      961 cccaggccca aaacgccgcg gctggtgcag gccgcgccgg gccccacgcc caccaacagg
     1021 gcggcggcgc cggtttccat gagttccaaa gcggcggaat aggtgacgac gttgccgatg
     1081 atcacgggga ttttcagctt ttggcaaagt ttcgccaagt ccaccacggg gcccttggag
     1141 gacttgaaac gggccgtggt cacggtgcct tgaatgatga agaggtcggc gcccgcgtcg
     1201 gcggcgattt ttcccacccg ctcggcgttt tggggaatgg aggacacggc gcagagcacg
     1261 ccgcccttct tgatttcctt cacgcgctga gcgacgagct tgtctttgac cggctctttg
     1321 gagtagatgt cctggaccag cttggtggcc acttccggcg tggcttcggc gattttcttg
     1381 aagagcggtt ttgggttttc gtagcgggtt tggacgccgt ccaggttcaa gacggccaag
     1441 ccccccagcc gtcccatggc gacggcgaat tccacgtcca ccaccccgtc catggcgctg
     1501 gccaaaatgg ggatgtccaa cttcacgggc cccaggcgcg tcgaaacgtc cgcgtcttcg
     1561 gggttgaccg tcaccgtgcc cggcaccaac gccacttcgt caaacccgta ggcccggcgg
     1621 gcctccctat cgcgaccgat gaaaatgcca tgaacgacct ccctgagaat ttcgaaaatt
     1681 atttgacgac cacga
//
LOCUS       DAOQLS010000514         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_53227_length_1692_cov_4.846060, whole genome shotgun sequence.
ACCESSION   DAOQLS010000514 DAOQLS010000000
VERSION     DAOQLS010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53227_length_1692_cov_4.846060"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="PK362_09010"
     CDS             complement(<1..1678)
                     /locus_tag="PK362_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC75004.1"
                     /translation="MTPEGAAAKGSWAGAGTPKRAEIPAAGAASEIAPDFVARSLVGF
                     LAVSSCFYALAYFFPFVDRRVIAPDYVRWIKEFVWGGVLLFGVRAWFRRRPPLASLWS
                     SWPRRLTTQFMVVYGVLLVCHALHLFSERFVIVYAKNFLVYSPALLLAGILADNPLAL
                     SRAVGKTLVGLGTVQVVVGFAVMAFFPGAAFWRDDPTTGFHPLVGFLGNPNRFGLFLN
                     VGAAWLLVESLAARVFWRGAAAAAGLSVFAVAVAYTGAVSQLVVTLGLLAYGAFVARF
                     ALGTRWPRILAVVGLTLAALGLFAGPFLLKVPVVEDALRFFSIGTHAPAPFCTAIGNR
                     LRQFYWIRNYALNASGPSLLFGSFSSRGVFLLDSEWSDVFLNWGLVGMASLLSMVVGL
                     GWGSVRAARTSTWSLERRWVSAAHHLALAVFGATFLIDSGLYDFPTSFLLFLVAGLLA
                     ALLTEAPDIRAAATVPAGPRPGRGARGEPSPDGPAAGPYPSSPVVSIVTPAYNAAAYI
                     DETIASVQSQTFADWEMWVVDDCSKDDTAARVAARAARDPRVRLIRQPRNG"
BASE COUNT          387 a          587 c          538 g          180 t
ORIGIN      
        1 ccccgttccg cggttggcgg atcaaccgga cgcgcggatc ccgcgcggcc cgggcggcca
       61 cccgggcggc ggtgtcgtcc ttggagcaat cgtccaccac ccacatttcc caatccgcaa
      121 aggtctgcga ctgcacggag gcgatggttt cgtcgatata agccgccgcg ttgtacgccg
      181 gcgtcacgat cgacaccacc ggggacgacg ggtacggccc cgccgccggg ccgtccggcg
      241 acggctcccc gcgcgccccc cgcccgggcc ggggacccgc ggggaccgtc gccgccgccc
      301 gaatatccgg cgcttcggtc aacaaagcgg ccaggagacc cgccaccaaa aacaataaaa
      361 aactggtcgg aaaatcgtaa agaccgctgt cgattaaaaa cgtcgccccg aagaccgcca
      421 aggccaaatg gtgggcggcg gaaacccaac gacgctccag gctccaggtc gaggtccgtg
      481 cggcgcgaac ggacccccac ccgagcccga cgaccatgga cagaaggctc gccatgccga
      541 cgaggcccca attcaaaaag acgtcgctcc attcactgtc caaaagaaac acgccccggg
      601 aagagaaact gccgaagagc agggagggcc cgctggcgtt cagggcgtaa ttgcggatcc
      661 agtaaaattg gcggaggcgg ttgccgatgg cggtgcagaa aggggcgggc gcgtgcgtcc
      721 cgatggaaaa aaaacgcagc gcgtcttcca ccacgggaac cttgaggaga aaggggcccg
      781 cgaacagtcc cagagccgcc agggtcagtc ccacgaccgc caggattcgg ggccaccggg
      841 ttcccagggc gaaacgcgcc acgaaggccc cgtaggccaa caagcccaac gtgaccacca
      901 gttgactgac ggcgcccgta tacgccacgg ccacggcaaa gacggacaag cccgcggccg
      961 ccgcggcccc ccgccaaaac acacgggccg ccagcgactc caccagcaac cacgcggccc
     1021 ccacgttgag gaacagtcca aaacggttcg gattgcccag gaaccccacg agcggatgga
     1081 agcccgtcgt gggatcgtcc cgccaaaagg cggcccccgg gaaaaaggcc atgaccgcaa
     1141 atccgaccac cacctgaacg gtcccgagtc ccacgagggt tttgcccacg gcccgggaga
     1201 gggcgagggg attgtcggcg aggatccccg ccagcaacag ggcgggactg tagaccagga
     1261 aatttttggc gtaaacgatg acgaatcgtt ccgagaaaag atgcagggcg tggcacacga
     1321 gcaacacccc gtagacgacc ataaattggg tcgtcaagcg gcggggccag gaggaccaaa
     1381 gggaggcgag gggcggccgt cggcggaacc aggcccggac cccgaaaagg aggacgccgc
     1441 cccaaacaaa ttccttgatc caccggacgt agtccggggc aatgacccgc cggtccacga
     1501 acggaaaaaa ataagccagg gcgtaaaaac aggaggacac cgccagaaac cccacgaggg
     1561 aacgggccac aaaatcgggc gcgatttccg acgcggcccc ggccgccggg atctcggctc
     1621 gtttcggggt tcccgccccg gcccacgagc ccttcgccgc cgcgccctcc ggggtcacgc
     1681 gccccccccc gc
//
LOCUS       DAOQLS010000515         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_53434_length_1689_cov_2.660343, whole genome shotgun sequence.
ACCESSION   DAOQLS010000515 DAOQLS010000000
VERSION     DAOQLS010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53434_length_1689_cov_2.660343"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK362_09015"
     CDS             <1..387
                     /locus_tag="PK362_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75005.1"
                     /translation="PVHHERHSHYMVLHADVPGLENREAEFLAGLALWHKAGKPEKKE
                     WPFSDKTKRSPFLKILALLRIVDALDKPRSHRVSLSTVRRRPGAVRLFLRGQRAAVDL
                     AVLRVEQKKELFEKVFNADLSAVGPG"
     gene            complement(397..891)
                     /locus_tag="PK362_09020"
     CDS             complement(397..891)
                     /locus_tag="PK362_09020"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNC75006.1"
                     /translation="MAKEPRHTLLILRHAHRSLSHPGLDNGLSSKGREQARLILKLFK
                     SFFGRPPKARLLSSPKKRCLETLRPIAKHTGLRTETDDRLVEQRPAETQSQFEDRVRR
                     FVNEQRKAASTLTVVCSHGDWVPVALRLATGGRMRLKKGGWAQVEFEKGTAPRLSWLL
                     QKLD"
     gene            complement(884..>1689)
                     /locus_tag="PK362_09025"
     CDS             complement(884..>1689)
                     /locus_tag="PK362_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75007.1"
                     /translation="GGTEKLYTHLSTGNYNAATARAYEDLAVLTSDPSLGEDARRFFD
                     AVCAGEIPTQFQSLVPAPAKLHRRLLQLIQQESEAAKAGRPAYLFAKVNALVDRTIVE
                     SLYAASQAGVAVDLVVRGACALIPGVKGLSENIRVISLVDRFLEHSRIYYFAAANRMY
                     LSSADWMPRNFFSRLELAFPVRDERLFRYIRDVVVPVYLADTVKGRELTPQGTWRKRS
                     VADTATPRARRTSPGFVGQSIRAQFYFEELANRGYRGTPLSRVPETVGG"
BASE COUNT          319 a          519 c          537 g          314 t
ORIGIN      
        1 cccgtccacc acgaacggca ttcccactac atggttctcc acgcggacgt gcccggtttg
       61 gaaaaccggg aagccgaatt tttggccgga ttggcccttt ggcacaaggc cgggaaaccc
      121 gaaaagaagg aatggccctt ttcggacaaa accaaacggt cgccgttcct gaaaattttg
      181 gcgctcctgc gcatcgtcga cgccctggac aagcctcgtt cccatcgggt ttccctttcg
      241 acggtgcggc gacgcccggg ggccgtccgg cttttcctgc ggggacaacg cgccgccgtc
      301 gatttggcgg tgcttcgcgt ggaacaaaaa aaagaattgt tcgaaaaggt gtttaacgcc
      361 gatttgagcg ccgtcggtcc cggctgaggg ccggcgtcaa tccaatttct gcagcaacca
      421 ggagaggcgg ggggccgtgc ccttctcgaa ttcgacctgg gcccaccccc cttttttcag
      481 ccgcatccgc ccacccgtcg ccaaccgcag cgccaccggc acccaatcgc cgtgggagca
      541 caccaccgtc aaggttgagg ccgctttccg ttgttcgttg acgaatcggc ggacccggtc
      601 ctcgaactga ctctgtgttt cggcgggccg ctgttccacc agacgatcgt cggtctccgt
      661 ccgaagaccc gtgtgtttgg caatggggcg gagagtttcc aggcaccgct ttttggggct
      721 ggagagcagg cgggctttgg ggggacgacc aaagaatgat ttgaacaatt tgagaatcag
      781 acgggcctgc tcccgcccct tcgagctcag cccgttgtcc aggccggggt gcgacagcga
      841 ccggtgggcg tggcgcaaaa tcaaaagagt gtgtctcggt tccttagcca ccgaccgtct
      901 ccgggacgcg ggacaggggc gtcccgcgat agccgcggtt cgccaactct tcgaaataaa
      961 attgggcgcg gatcgattgg cccacaaagc cgggggaggt ccgccgggcc cggggcgtgg
     1021 ccgtgtcggc caccgaccgt tttcgccagg ttccctgggg ggtcaactcc cggcctttga
     1081 ccgtgtcggc cagatagacc gggacgacca cgtcccgaat gtagcggaag aggcgctcgt
     1141 cccggacggg aaacgccaac tccagccgtg aaaaaaaatt gcggggcatc caatcggcgc
     1201 tggataaata cattcgattg gcggcggcga aataataaat tcggctgtgt tccagaaatc
     1261 ggtccacgag agaaataacc cgaatgtttt cgctcaaccc tttgacgccg gggatcaacg
     1321 cgcaggcccc gcgcaccacg agatcgacgg cgacgccggc ctgggaggcc gcgtacaggc
     1381 tttccacgat cgtccggtcg accaaggcgt tgaccttggc gaaaaggtag gcggggcggc
     1441 cggccttggc ggcttcggac tcctgttgga tcaattgcag aagcctccgg tggagtttcg
     1501 cgggggccgg caccagggac tggaattgcg tcgggatctc ccccgcgcaa acggcgtcga
     1561 aaaaccgccg ggcgtcttcg cccagggacg ggtccgaggt caacacggcc aaatcctcgt
     1621 aggcgcgggc cgtggccgcg ttgtaattcc ccgtggacag gtgggtgtag agtttttcgg
     1681 ttcccccct
//
LOCUS       DAOQLS010000516         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_53657_length_1685_cov_4.900000, whole genome shotgun sequence.
ACCESSION   DAOQLS010000516 DAOQLS010000000
VERSION     DAOQLS010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53657_length_1685_cov_4.900000"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK362_09030"
     CDS             complement(<1..215)
                     /locus_tag="PK362_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNC75008.1"
                     /translation="MFGKVQRIHFVGIGGAGMSGIAEILLVLKCQVSGSDLKVTDVTE
                     RLRGMGAKVFAGHDERHVEGADVLVTST"
     gene            complement(215..1399)
                     /locus_tag="PK362_09035"
     CDS             complement(215..1399)
                     /locus_tag="PK362_09035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNC75009.1"
                     /translation="MTPPDPTAPPTVRLKAHEDRRLRRGHLWVFSNEVQSAPEGLEPG
                     SLVRFESSREEPLGVGFYNPQSLIAGRVLDRRPVPIDVAFFIGRLRRARELRERLFPD
                     GAYRWVFGESDDLPGLVVDRFGDAVVVESFCAGMDRLTPLVLEALQTFGPWKAVVLKN
                     DAAARRLEKLPESVAAVAGAPDRPHWFQADGLHLAADLLEGQKTGYFFDQRANRASAA
                     RLAKGQSVLDVFCHTGGFGLACAQAGATRVTGIDESDPALNLARLAAEKNALGDRVTF
                     TRADAFDYLMTSKENHGLVVVDPPRFASNKKNLPQAIKAYVRLNTLALRRVSGGGFLA
                     TASCSQHVDREEFRQILSRAVHESGRRAVVIHTAGAGPDHPVRPSMPETDYLKFAILH
                     VT"
     gene            complement(1396..>1685)
                     /locus_tag="PK362_09040"
     CDS             complement(1396..>1685)
                     /locus_tag="PK362_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75010.1"
                     /translation="EKAFAKLVLTSQTTDEEMEETVNLLLRLSPPPPLVLQPVTPVTD
                     LDFRLQDAQSAAPAAVIPPPPARLVSWWDWARQKLPSVKLISQMHPIWGLP"
BASE COUNT          315 a          496 c          593 g          281 t
ORIGIN      
        1 gtcgacgtga ccaacacgtc ggccccttcc acgtgccgtt cgtcgtgacc ggcgaacacc
       61 ttggcgccca ttccccgaag ccgttccgtg acgtcggtga ctttgagatc gcttccggag
      121 acctggcact tcaggaccaa aagaatttcg gcgatgccgc tcatgccggc gccgccgatt
      181 ccaacgaaat ggatgcgttg aacttttccg aacatcaggt gacgtggagt atagcgaact
      241 ttaaataatc cgtctccggc atggaagggc ggacggggtg atcgggaccg gccccggcgg
      301 tgtggatcac cacggcccgg cggcccgatt cgtggaccgc ccgggacaaa atttgtcgaa
      361 actcctcccg gtccacgtgc tgggaacagc tggccgtggc caaaaaccca ccgccggaga
      421 cccgacggag cgccagggtg ttcaggcgca cgtacgcctt gatggcctgg gggagatttt
      481 tcttgttgga ggcgaaccgc ggggggtcga caacgaccag cccgtggttt tccttggagg
      541 tcatcaaata atcgaaggcg tcggcccgcg taaaggtcac gcggtcgccc agggcgtttt
      601 tctcggcggc gaggcgggcc agattgagcg cggggtccga ttcgtcaatg cccgtcaccc
      661 gggtcgcccc ggcctgggcg caggccaacc cgaagccgcc cgtgtggcaa aaaacgtcaa
      721 ggaccgattg gcctttggcc agccgcgcgg cggaagcgcg gttcgcccgc tgatcaaaaa
      781 agtagccggt tttttgacct tccaagaggt ccgcggcgag atgaagaccg tcggcttgaa
      841 accaatgggg acggtcggga gcgccggcga ccgccgccac cgattccggc aatttttcca
      901 ggcgccgggc ggcggcgtcg tttttaagaa cgaccgcttt ccaggggccg aaggtttgaa
      961 gcgcctccag gacgaggggc gtcagccggt ccatgccggc gcaaaaggac tccaccacga
     1021 cggcgtcgcc gaagcggtcg accacgagcc ccggcaaatc gtcggattcc ccgaagaccc
     1081 agcggtaggc gccgtcgggg aaaaggcgtt cccgaagttc ccgggcccgt cgaaggcgtc
     1141 cgatgaaaaa tgcaacgtcg atggggacgg ggcggcggtc caggacccgg ccggcgatga
     1201 gggattgggg gttgtaaaaa ccgacgccca ggggttcttc ccgggaggat tcgaatcgga
     1261 ccagggaccc cggttccaag ccctcggggg cggattggac ctcgttggaa aacacccaga
     1321 gatggccccg gcgcaggcgg cggtcctcgt gggctttcag ccggacggtc gggggggccg
     1381 tgggatcggg gggtgtcacg gcaggcccca gatcgggtgc atttgggaga tcagtttgac
     1441 cgacggaagt ttttggcggg cccaatccca ccaggatacg aggcgggcgg gcggcggcgg
     1501 gatcacggcc gcgggcgccg cggactgggc gtcctgaagt cggaaatcca aatcggtcac
     1561 gggggtgacc ggttggagca cgagcggggg cgggggcgaa agccggagaa gaaggttcac
     1621 ggtttcttcc atttcctcgt cggttgtttg ggaagtgagg accaatttgg cgaaggcttt
     1681 ctccc
//
LOCUS       DAOQLS010000517         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_54240_length_1676_cov_4.378162, whole genome shotgun sequence.
ACCESSION   DAOQLS010000517 DAOQLS010000000
VERSION     DAOQLS010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54240_length_1676_cov_4.378162"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PK362_09045"
     CDS             <1..508
                     /locus_tag="PK362_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75011.1"
                     /translation="PARAARAMVLPDGKKEVRFQLEAQRITFFQDRSGGVAESSAPLS
                     MADAGGGVFIRSEESAPRPRRTELWSGRAVFDGREGRLTLENGFRPPPGFSNTMPPSE
                     SDRPFCRQTVGREIRDVSGEVMTYRPSTRRLVVERNAMARVLFEAKAAAVESKLKKEK
                     KKARGPAR"
     gene            492..1211
                     /gene="lptB"
                     /locus_tag="PK362_09050"
     CDS             492..1211
                     /gene="lptB"
                     /locus_tag="PK362_09050"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNC75012.1"
                     /translation="MALRAEDLRKRYGSRLVVDGVSVEVHPGEIVGLLGPNGAGKTTS
                     FYMTVGLVRPDGGRVLIDDQDVSALPMYRRARAGLGYLAQEPSIFRRLSVEDNLNAIL
                     EHTPLSPAQRSEKRDRLLADLGLTRLGKQMAHTLSGGEKRRTEIARALTTDPRYLLLD
                     EPFVGIDPLAVNDIQEVLGELKKRGLGLLITDHNVQATLEIVDRAYIIYAGKILVHGK
                     AQELLQSAEARRVYLGEKFHL"
     gene            1307..>1676
                     /gene="raiA"
                     /locus_tag="PK362_09055"
     CDS             1307..>1676
                     /gene="raiA"
                     /locus_tag="PK362_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNC75013.1"
                     /translation="MQIHITARHIELTPALADYARKKLERVARHFEIVLRAQVILAVE
                     KHRHSAEVVVHAQGHHDFRAREVAGDLYAAIDLAAEKLQKHLSREKDRRVRGRRGAKP
                     GEERGADRFAAPAAPREPVLT"
BASE COUNT          317 a          528 c          543 g          288 t
ORIGIN      
        1 cccggcccgg gccgcccggg cgatggtctt gccggacggt aaaaaggaag tgcggtttca
       61 attggaagcc cagcgaatca cctttttcca ggaccgatcg ggcggcgtgg ccgaatccag
      121 cgccccgctc tccatggccg acgcgggcgg cggcgtgttc atccgttccg aggagtcggc
      181 gccccgaccc cggcggacgg aattgtggtc tggacgggcc gtgttcgacg gacgcgaggg
      241 tcgcctgacg ttggaaaacg gttttcgccc gccgccgggg ttttcgaaca cgatgccgcc
      301 gtccgaatcg gaccggccct tttgccgtca aacggtcggc cgggaaattc gggacgtgtc
      361 gggcgaggtg atgacgtatc gcccgtccac ccgccgcctg gtggtggaac ggaacgccat
      421 ggcccgggtg ctgttcgagg ccaaggcggc ggcggtcgaa tccaaattga aaaaggagaa
      481 aaagaaagcc cgtggccctg cgcgctgaag accttcgtaa aagatacggc tcccgtctcg
      541 tggtggacgg cgtgtccgtg gaggtgcacc cgggcgagat cgtcggcctt ctgggaccca
      601 acggcgcggg caagaccacg agtttttaca tgacggtggg cctggtccgg cccgacgggg
      661 gccgcgtttt gatcgatgac caggacgtgt cggctctgcc gatgtaccgc cgggcccggg
      721 ccggtctggg gtatctggcc caggaaccgt ccattttccg tcggctttcg gtggaagata
      781 atttgaacgc catcctggag cacaccccgc tttccccggc ccagcggtcg gagaaacgcg
      841 accggctgct ggcggacctc ggtttgacgc gcctgggaaa acaaatggcc cacacgctct
      901 ccggcgggga aaagcgccgc acggaaatcg cccgggcgct cacgaccgat ccccgctatc
      961 tgcttctgga cgagcccttc gtcggcatcg accccctggc ggtgaacgac attcaagagg
     1021 ttctggggga actcaagaag cgggggttgg ggcttttgat cacggaccac aacgtgcagg
     1081 ccaccctgga aatcgtcgac cgtgcctaca tcatttacgc cgggaaaatt ctcgttcatg
     1141 gaaaggccca ggagctttta cagtccgccg aggcgcggcg ggtttacttg ggagaaaaat
     1201 tccatctttg agacggaaac gcttcggcgc cctggtttga cacgccgagg gtgaaattgt
     1261 aaagtgaatt gatttcaata cccgttttta tcgaaggatg accgccatgc agattcacat
     1321 cacggcccgg cacatcgaat tgacccccgc gttggccgac tacgcgcgaa aaaaactgga
     1381 acgggtcgcc cgccattttg aaatcgtcct gcgcgcccaa gtcattctcg ccgtcgagaa
     1441 gcaccgccac agcgccgagg tcgtcgttca cgcccagggc caccacgact tccgggcccg
     1501 ggaagtggcc ggcgatctgt acgcggccat cgacctggcg gcggaaaaac ttcagaaaca
     1561 tttgtcccgc gaaaaagacc gccgcgtgcg cggacgccgc ggcgccaaac ccggcgagga
     1621 acgcggcgcc gaccggttcg cggcccccgc cgcgccccgg gagcccgtgc tcaccc
//
LOCUS       DAOQLS010000518         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_54445_length_1673_cov_3.580346, whole genome shotgun sequence.
ACCESSION   DAOQLS010000518 DAOQLS010000000
VERSION     DAOQLS010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54445_length_1673_cov_3.580346"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..612)
                     /locus_tag="PK362_09060"
     CDS             complement(43..612)
                     /locus_tag="PK362_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNC75014.1"
                     /translation="MGNKKSGGSLDRRLRRVRLFAMDVDGVLTDGGIIVLNSGEEVKV
                     WNVKDRIAFFILKRFGDRFPVAWITGRKSRQVADRAKEVGVAALFQNCDHKGLALESV
                     LDKLKLTPAESLFMGDDLVDLPALRRAGVAVCPADAHPEVKKACHWVTRAAGGRGALR
                     EVVDRVLTVRGLMPAVVAQFENPRRRPNP"
     gene            complement(602..1576)
                     /locus_tag="PK362_09065"
     CDS             complement(602..1576)
                     /locus_tag="PK362_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010629177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNC75015.1"
                     /translation="MNASGWRARARRTLAIESRAVAAQARHLDAAFLKAAQRLKATKG
                     RVVVMGIGKSGLIGRKLAATLASTGTPAYFVHPTESLHGDLGMIRPGDTVLALSHSGE
                     TEELNKILAFLRERGVFLIGLTGRPRSALGRAADLIINTAVPEEACPFNITPTASTTA
                     MLALGDALALLVMEMRGFGREDFARLHPAGSLGKRLLLRVKDLMRKGAENPLVPQTKK
                     VADALQVMTRTRVGAASIVDGRGRLVGVFTDGDLRRRLPADPSLLSRPLTAVMTRSPR
                     TVAPEDLAAAVAGLFRQFGLDNFPVVDRQGRPVGLLDEKDLLEEGLGE"
BASE COUNT          305 a          550 c          564 g          254 t
ORIGIN      
        1 ccccgatcat ggccagggcg acgaacgcgc ccggtccccg gttcacgggt tcggccgtcg
       61 acggggattt tcgaattgag cgaccaccgc gggcatcagc ccccgaacgg tcaacacccg
      121 gtccaccacc tcccggaggg cgccccggcc cccggccgcc cgggtgaccc aatgacaagc
      181 tttcttcact tccggatggg cgtcggcggg acaaacagcc acgccggccc ggcgcagggc
      241 gggcaggtcc accaaatcgt cgcccatgaa cagcgattcc gcgggggtca attttaattt
      301 gtcgaggacc gactccaggg cgaggccctt gtgatcgcaa ttttgaaaga gcgcggccac
      361 gcccacttcc ttggcccgat ccgccacctg gcgggattta cgacccgtga tccaggccac
      421 ggggaaccgg tccccgaacc gtttcagaat gaaaaaggcg atgcggtcct tcacgttcca
      481 gaccttgacc tcctccccgg agttcaaaac gatgatgccc ccatcggtca gcacgccgtc
      541 gacgtccatg gcgaagagcc ggacgcgtcg aagccgccga tcgagggagc cgccggactt
      601 tttattcccc aaggccttcc tccagcaaat ccttttcgtc cagcaagccc acgggccggc
      661 cttgacggtc caccaccgga aaattgtcca acccgaattg gcggaaaagc cccgccacgg
      721 cggcggccaa atcctcggga gcgacggtgc ggggcgagcg cgtcatgacg gcggtcaggg
      781 ggcgggagag gagggacggg tccgcgggca atcggcggcg caagtcgccg tcggtgaaca
      841 cccccacgag gcggccccgg ccgtccacga tggaggccgc gcccacccgc gtgcgggtca
      901 tgacctggag agcgtcggcc acttttttgg tttggggcac caggggattt tcggccccct
      961 tccgcatcaa atccttcacc cgcaacaaca ggcgcttccc cagggacccc gccggatgga
     1021 gacgggcgaa atcctcccgg ccgaacccgc gcatttccat gaccaggaga gccagagcgt
     1081 cgcccagggc cagcatggcg gtggtggagg cggtgggggt gatgttgaaa gggcaggcct
     1141 cttcgggcac cgcggtgttg atgatcaagt ccgccgcgcg tcccagggcg gagcggggcc
     1201 ggcccgtgag gccgatcaag aagaccccgc gttcccgcag gaacgccaat attttattga
     1261 gctcctcggt ttccccggaa tgggaaagag ccagaaccgt gtcccccggg cggatcatgc
     1321 ccaggtcgcc gtgcaggctt tccgtcgggt ggacgaagta ggcgggggtg cccgtggaag
     1381 ccaaggtggc ggcgagcttc cgcccgatga gaccggattt gccgatgccc atgaccacca
     1441 cgcggccctt ggtggccttg aggcgctggg cggcttttaa aaaggccgcg tccaaatggc
     1501 gggcctgggc ggccacggcc cgggactcga tggccagggt gcgccgggcg cgggcgcgcc
     1561 agccggacgc gttcattttt tgagccaggg ggtcatctcc ggcggaatgt cgcggaggcg
     1621 gtagggttcc ggaaggcggg aaagaaaatg atccaccgcg aagaacacga tgg
//
LOCUS       DAOQLS010000519         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_54580_length_1671_cov_3.937500, whole genome shotgun sequence.
ACCESSION   DAOQLS010000519 DAOQLS010000000
VERSION     DAOQLS010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54580_length_1671_cov_3.937500"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PK362_09070"
     CDS             <1..457
                     /locus_tag="PK362_09070"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC75016.1"
                     /translation="PNSIFPIIGASGAIYGLLVAFAMMYPMSVFYIYFMIPMRAWQAV
                     ILFAAIEFLMGFSGTHSRLANIAHLGGMLTGFVYLKSATVRYDFRRWIGRMGDWSAQR
                     KEKKAVVQFHELGQEVDRILEKISADGIASLTPEEQRLMERYSRMKTKR"
     gene            468..1553
                     /locus_tag="PK362_09075"
     CDS             468..1553
                     /locus_tag="PK362_09075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC75017.1"
                     /translation="MSDDKSREGAIDVLRGVSIAAVLFYHFELADPVSRFGVYGVALF
                     FMISGYCMLPSVRGSASLGDFLAKRYARLLPALWICAVITTAVERSFPLARPDRIHGY
                     MDMAKTMVFLPAVNLPTFLPRLWGGPVNHYAFVDGAYWSLLVEFKFYWLIGLAYFALR
                     RFNPFWVIYAVSWAGNALVGGFGGPDYWTDFFPYLPLFLVGMAGWEIRHGNRRRGGWQ
                     WLAALGALAALPWMGGEKAPSLPLHGLAVGTGVFCGLMLVFWALTDQYWERPIATRTQ
                     GRWLAALGTISYPLYLLHQDVGYVLFALFRWGESPGGRALLAVPLLGGAMAVHFAIEN
                     RFRPLLRKKLRPVFDGMFAPAGLGPRA"
BASE COUNT          264 a          496 c          531 g          380 t
ORIGIN      
        1 gcccaactcg attttcccga tcatcggagc ctcgggggcc atttacggtt tgcttgtggc
       61 cttcgccatg atgtatccca tgtccgtttt ttacatctac ttcatgatcc cgatgcgggc
      121 ctggcaggcg gtgatcctct ttgcggccat cgaatttttg atggggttct caggcaccca
      181 ctcgcgcctg gccaacatcg cccacctggg gggaatgtta acgggtttcg tatatctgaa
      241 atccgccacg gttcgttacg atttccgccg gtggatcggc cggatggggg attggtccgc
      301 ccaacggaag gaaaaaaagg ccgttgtcca gttccacgag ctgggacagg aagtggatcg
      361 aattctggaa aagatttccg ccgacggcat cgccagtttg acgcccgagg aacagcgcct
      421 gatggagcgc tattcccgga tgaaaaccaa acgctgacct tcctcccgtg agcgacgaca
      481 aatcccgcga gggcgccatc gacgtcctgc ggggggtcag catcgccgcg gtccttttct
      541 accacttcga attggcggat cccgtgagcc gcttcggcgt ctacggcgtg gcgctgtttt
      601 tcatgataag cggctactgc atgttgccga gcgtgcgggg gagcgcctcc ctgggcgact
      661 ttttggccaa gcgctacgcc cggctcctgc ccgccctctg gatatgcgcc gtgataacga
      721 cggcggtcga gcggagcttt cccctggccc gcccggaccg gattcacggc tacatggaca
      781 tggccaaaac catggtcttc cttccggccg tcaatcttcc aacctttctg ccgcgccttt
      841 ggggcgggcc ggtcaaccac tacgcctttg tcgacggggc ctattggtcc ctcctggtcg
      901 aattcaaatt ttattggttg atcggcctgg cgtactttgc cctgcgccgt ttcaatccct
      961 tttgggtgat ctacgccgtg agttgggcgg gcaacgcgtt ggtgggcggg ttcggcggcc
     1021 ccgactattg gacggatttt ttcccttact tgccgctctt cctggtgggc atggccggat
     1081 gggaaattcg ccatggaaac cgacggcgcg gcggttggca atggctagcc gccctgggcg
     1141 cgctggccgc tctgccctgg atggggggcg aaaaggcgcc gtcccttccc ctgcacggac
     1201 tggcggtcgg caccggcgtc ttttgcgggt tgatgttggt gttctgggcg ctcacggacc
     1261 aatattggga gcgacccatc gccactcgga cccaggggcg ctggctggcg gccctgggca
     1321 cgatttccta tccgctctat ctcctccatc aagacgtggg atacgttctg ttcgctcttt
     1381 ttcggtgggg ggaatctccg ggaggccggg cgctcctggc cgttccgctt ctcggcggcg
     1441 cgatggccgt tcattttgcc attgaaaatc gatttcgacc gctgttgcgc aaaaagcttc
     1501 ggccggtttt tgacgggatg ttcgccccgg cggggctggg tccacgggcg tgaagggaaa
     1561 ctaaagaaga ggggaaagca cccgacagag gccttcggcg aagtgtcgga ccggggcccg
     1621 ggcgggtcgg gcggaaacgg ccacggattg gcccgccgtt ttatcgaagg t
//
LOCUS       DAOQLS010000520         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_55078_length_1663_cov_5.038557, whole genome shotgun sequence.
ACCESSION   DAOQLS010000520 DAOQLS010000000
VERSION     DAOQLS010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55078_length_1663_cov_5.038557"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK362_09080"
     CDS             <1..419
                     /locus_tag="PK362_09080"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC75018.1"
                     /translation="QVLRLDPSNTLARDYMDRIRSGGSGPTPGGFSGGPRAPDDFVRA
                     VGYLKENRFVEAIEALERYLARNPTDSKADALLKETKTRQKEQLDLLYKEGLTAYAQG
                     MKNQAIQKWQEALRVDPDFVRAKQALVKTMQEVRGQ"
     gene            416..>1663
                     /locus_tag="PK362_09085"
     CDS             416..>1663
                     /locus_tag="PK362_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC75019.1"
                     /translation="MKTHRAVNFAIAILMAAPTLRAEEPRQENTLESVTVQTTGRFSK
                     VILQSARSLKYRDMKQESAVVLYMLEPTLSRKPPVEKTYSDLVDEIRYTYKGNRTPMP
                     GGNPELLEFVTIHLKETASANIVQKDWLLAVELRRGEAAADATPGLAPEPLTGSAPTV
                     KQKGIHALPPNPTLADFLEVGLANHAPLRIAQDELKLATARHVESMRNLLPSVTGKYT
                     FSTGQMVQDPTKPDDDVAFKRKELGLEMGMPLFHSGQNVYALRQAAYQKRVAQQNVAK
                     TKSDITFEIRRAYFNMVKAQRSLRVRRELNQRTEKIIEVSRKKKQLGLITQADALGAE
                     SLYSQSYYRLLSDEKELEIARLKLSALLNTNDPLPEVVPEPKQTTDSRTLLELSVPPE
                     SLIQLGMSHRPELLISEYTAAAQH"
BASE COUNT          399 a          510 c          461 g          293 t
ORIGIN      
        1 accaggtgct gcgtttggac ccctcgaaca ccctggcccg ggattacatg gaccgcatcc
       61 gttcgggggg gagcgggccg acgcccggcg ggttctcggg aggcccccgg gccccggacg
      121 attttgttcg ggccgtgggg tatctcaagg aaaatcggtt tgtggaagcc atcgaggcgt
      181 tggaacgcta tctggcccgc aacccgacgg attccaaagc cgacgccctg ctcaaggaaa
      241 ccaaaacgcg tcaaaaggaa caattggacc ttctctacaa agagggcctg acggcctacg
      301 cccaaggcat gaagaaccaa gccattcaaa aatggcagga agccctgcgc gtcgatccgg
      361 atttcgtgcg cgcgaagcaa gcgctggtta aaaccatgca ggaggttcgc ggacaatgaa
      421 aacccatcga gcggtaaatt tcgccatcgc gattttgatg gcggcgccca cccttcgggc
      481 ggaggaaccc cgccaggaaa acaccttgga atccgtcacc gtccagacca cgggtcggtt
      541 ttcaaaagtc attttacaat ccgcgcgttc gctgaaatac cgcgacatga aacaggagtc
      601 cgccgtcgtc ctttacatgc tggagccgac gttgtcgcga aagccccccg ttgaaaaaac
      661 ctatagcgat ctggtggatg aaattcgata cacctacaaa ggcaaccgga cgccgatgcc
      721 cggggggaat ccggaacttc tcgaattcgt caccattcat ctgaaagaga ccgcgagcgc
      781 caacatcgtc caaaaggatt ggttgttggc ggttgaattg cggcgggggg aggccgcggc
      841 cgacgccacg cccggcctcg cgccggagcc tttgaccgga agcgccccga ccgtcaaaca
      901 aaaagggatt cacgcgctcc cgcccaatcc gacgttggcg gatttcctgg aggtgggttt
      961 ggccaatcac gcccccctgc gcatcgccca ggacgaattg aagctggcca ccgcgcgcca
     1021 cgtggaatcc atgcggaatc tgcttccctc cgtcacgggg aaatacacct tttccaccgg
     1081 acaaatggtt caagacccga ccaaacccga tgacgacgtc gccttcaaac gcaaagaatt
     1141 gggattggag atgggaatgc cgttgttcca cagcggacaa aacgtgtacg cccttcggca
     1201 ggccgcttat caaaaacggg tggcccaaca gaacgtcgcc aagaccaaaa gcgacatcac
     1261 ctttgaaatc cggcgcgcct acttcaacat ggtcaaagcc caacgatccc tccgcgtccg
     1321 gcgggaactc aaccagagga cggaaaagat tatcgaggtg tcccgcaaaa agaagcagtt
     1381 gggtttgatc acgcaggcgg acgccctggg cgccgagtcc ctctatagcc agtcgtatta
     1441 ccgccttctt tcggacgaaa aagagctgga aatcgctcgg ttgaagctga gcgccttgct
     1501 gaacacgaac gatcctctgc ccgaggtcgt cccggaaccc aagcaaacga cggattctcg
     1561 aactctcttg gaattgagcg ttcctcccga atccctgatc caattgggga tgtcccaccg
     1621 ccccgaactg ctcatttccg aatacaccgc ggccgcccag cat
//
LOCUS       DAOQLS010000521         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_55089_length_1663_cov_4.298507, whole genome shotgun sequence.
ACCESSION   DAOQLS010000521 DAOQLS010000000
VERSION     DAOQLS010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55089_length_1663_cov_4.298507"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1663)
                     /locus_tag="PK362_09090"
     CDS             complement(<1..>1663)
                     /locus_tag="PK362_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75020.1"
                     /translation="GVSSTPASTLPGASNLVDEASHAVRARTAYNNVGLAAEYWEGTS
                     TNKEGAVQSLTHVHDINYNALGQQVKTISDLHKIGLDLDLFQTLQRDGMAYNSFGQII
                     GYVDHTTQNGLNVVTTVMDITYDREGRITSSKSVTNKQGEELQTFYTVGGNDLTPVQL
                     AAFLEAHPGKTVADLVTSGALKFERRKVSVNETVISVTSDLKYNKLNRVLSSKETVYN
                     TDGTTSVNVLSELEYDAQGRLVASQMSNHQFGPSQQMIYHLDGVELDAQDLATLLKSE
                     DDKEGASTTGATFWDLLESGRLTVETVDVQFDKTTSTSRTNMRYNENGQLAHYRDESA
                     DPENRIEKQVNNVDVTYTMRGFVHTQTTSSRIDNTDGGWTESTGVLTFHYDAATDLLL
                     GAESHSTFTTQPPQVWTDENQDGEKDTLVWLPPVTGASHQTFGAVNGQIRLLNQISEQ
                     DIKNIENANGLTHSVTQSVYQYNAKGRLLGSVSSGQTVGNDGFGNLNEGRLQQLSVVI
                     NGEMKTIISHTDSTAKTAEGSENRSVTDLIYTYDALGHVTGAAGRG"
BASE COUNT          288 a          438 c          523 g          414 t
ORIGIN      
        1 gccccgcccg gcggctcccg tcacgtggcc cagagcgtcg taggtgtaaa tgaggtccgt
       61 gacggagcgg ttttccgatc cttcggccgt cttggcggtg ctgtccgtgt ggctgatgat
      121 ggtcttcatt tcaccgttga tcaccacgga gagctgttgc aagcgacctt cgttgaggtt
      181 gccaaatccg tcgttgccga cggtttggcc cgaagacacc gaccccaaca agcggccttt
      241 cgcgttgtat tgataaacgg attgagtcac ggaatgcgtc agaccgttgg cgttttcaat
      301 gttcttaatg tcctgctctg aaatttgatt caggagacgg atttgtccgt tgacggcccc
      361 gaaggtttgg tgggaggccc cggtcacggg cggcagccac acgagggtgt ctttttcccc
      421 gtcctggttt tcgtccgtcc agacctgggg cggttgggtg gtgaaggtgg agtgggactc
      481 ggcgcccaac aacaggtccg tggcggcgtc gtaatggaag gtcaacaccc cggtggattc
      541 ggtccagccg ccgtcggtgt tgtcgatccg ggaagacgtg gtttgcgtgt ggacgaaccc
      601 gcgcatggtg taggtcacgt caacgttgtt gacttgtttt tcaatccggt tttccggatc
      661 ggcgctttcg tcccggtaat gggccaattg accgttttcg ttgtaacgca tgttcgtgcg
      721 cgaggtgctc gtggtcttgt cgaactggac gtcgacggtt tcaaccgtca ggcggccgct
      781 ttccaggaga tcccagaagg tcgcgccggt ggtcgaagcg ccttccttgt cgtcctcgct
      841 tttcaacagg gtcgcgagat cctgggcgtc caattcaacg ccatccaagt ggtaaatcat
      901 ttgttgggaa ggcccgaatt gatggttgga catttgggaa gccaccagcc gaccttgggc
      961 gtcgtattcc aattccgaaa ggacgttgac ggacgtggtc ccatccgtgt tgtaaacggt
     1021 ttcctttgaa gagaggaccc ggttcagctt gttgtatttc aaatcgctcg tgaccgaaat
     1081 caccgtttcg tttaccgaca cttttcgccg ctcaaatttg agcgcgccgg aagtgaccaa
     1141 gtcggccacg gttttgccgg ggtgggcttc gaggaaggcc gccaattgga ccggcgtcaa
     1201 gtcgtttccc cccacggtgt agaaggtttg aagttcctcg ccctgtttgt tcgtaacact
     1261 cttggaggag gtaatgcggc cctctcggtc gtaggtgatg tccataaccg tggtgaccac
     1321 attgagcccg ttttgggtcg tgtggtccac gtagccgatg atttggccga aagagttgta
     1381 ggccatccca tcgcgttgga gcgtctggaa aaggtccaga tcgagaccga tcttgtgaag
     1441 atccgagatc gttttcacct gctgacccag cgcgttgtaa ttaatgtcgt gcacgtgggt
     1501 gagggattgg acggcccctt ccttgttggt cgatgtccct tcccaatatt ccgccgccag
     1561 cccaacgttg ttgtaggccg tgcgggcgcg cacggcgtgg gaagcttcgt cgacgagatt
     1621 ggaagccccc ggcaaggtgg aggcgggggt ggaggacacc ccg
//
LOCUS       DAOQLS010000522         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_55286_length_1660_cov_3.109034, whole genome shotgun sequence.
ACCESSION   DAOQLS010000522 DAOQLS010000000
VERSION     DAOQLS010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55286_length_1660_cov_3.109034"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1660)
                     /locus_tag="PK362_09095"
     CDS             complement(<1..>1660)
                     /locus_tag="PK362_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75021.1"
                     /translation="LTEYGLLWAGAAVLWRGAQERRWTPAGFWALGVGGGLLVALPQI
                     LPSWELAGRSLRSAITEADAARYLLTPALLLKIAIPNLLDKAASPFNPSAFGAEFWPV
                     QRNWLSTFYVGTAPLLLAVGAAFSRPRRKIFWIGSGAFFLLLAMGCEPIFTVVRRFVP
                     GFRYMTHFSNAAVVSVLAVALLAAAGGSDAAARKTAARFAGGLAALCFVGTLWAASRT
                     WLIGAMLGTAELTAVQDQRVRWAFVETGLIAIGVGLMAPRPRWGTAGLLCFTLLELAW
                     NAAGLHPLAPAGFFRERVALAAQYQNQPRRFALTPAALDTRGMAGDNLVDGYRSLRQA
                     GFPTVMLPYRAPNAWSYEVFGLSDFAAFRRRLSREDPGGPVLDFLGVEGLVAHGPLSA
                     PRVPVARSANALFYRNPGALERVTAVALSTTVVDASARLQYLSTAWNPRREVVLEGPA
                     SAPPTDTPPEIHWTDRPGRVEFKGRGVGWAVQSAPFYPGWEVYVNGSRETNRRANHAF
                     QAVALPAGEWRGAWIYRPAMLRWGVLLALTGLAGLGADAVRRARR"
BASE COUNT          327 a          568 c          562 g          203 t
ORIGIN      
        1 ccgccgcgcc cggcgcacag cgtcggcgcc caatcccgcc aggcccgtca gggccagaag
       61 aaccccccac cgaagcatcg ccggccgata gatccaggcc ccccgccatt ccccggcggg
      121 caaggccacc gcctgaaagg cgtggttcgc ccgtcggttg gtttcccggg atccgttgac
      181 gtaaacctcc catcccggat aaaacggcgc gctttgaacc gcccagccga cccctcggcc
      241 cttgaattcc acccggcccg gacgatcggt ccaatggatt tccgggggcg tgtcggtcgg
      301 cggagccgac gccggcccct ccagcaccac ttcccggcgg gggttccagg cggtggagag
      361 gtattggagg cgagccgaag catccaccac ggtggtcgag agcgccacgg cggtgactcg
      421 ctcgagagcg ccggggttgc ggtagaacaa ggcgttggcg gaccgggcga cgggcacccg
      481 gggcgccgaa aggggcccgt gggccacgag accttccacg cccaggaaat cgagtacggg
      541 gccgcccggg tcctcccggg agagtcgccg gcggaaggcc gcgaaatccg aaaggccgaa
      601 gacttcgtag gaccaggcgt tgggcgcgcg gtagggcagc atcaccgtcg ggaatccggc
      661 ctgccgcagg ctccggtagc cgtccacgag attgtccccg gccatgcccc gggtgtccaa
      721 ggccgccggc gtcagggcga aacgccgcgg ttgattttga tattgggccg ccagggcgac
      781 gcgctcccgg aaaaagcccg ccggcgccag gggatgaagt ccggcggcgt tccaggccaa
      841 ttccagcagg gtaaaacaaa gaagccccgc cgttccccac cggggacggg gcgccataag
      901 gcccaccccg atggcgatca atcccgtttc aacaaaggcc caccgaaccc gctggtcctg
      961 gacggcggtg agttccgccg tgccgagcat cgcgccgatc aaccaagtcc gggaagcggc
     1021 ccacagggtc ccgacgaaac aaagggcggc gagccccccg gcgaagcgag ccgccgtttt
     1081 tctcgccgcg gcgtccgatc cgccggcggc cgccagcagg gcgacggcca gaaccgaaac
     1141 gacggcggcg ttggaaaaat gggtcatgta gcggaacccg ggaacgaaac gccggacgac
     1201 cgtgaaaatt ggttcgcacc ccatggccaa aaggagaaag aacgccccgc tcccgatcca
     1261 gaagattttc cggcgggggc gggaaaaagc cgctcccacg gccaggagaa gcggtgccgt
     1321 tccgacgtaa aacgtcgaaa gccaattccg ctgaaccggc caaaattccg ctccgaaggc
     1381 cgatggatta aacggcgacg ccgccttgtc caggaggttc ggaattgcga tttttaaaag
     1441 caaggccggg gtcaaaagat agcgggccgc gtcggcctcg gtgatggcgg accggaggga
     1501 tcgtccggcc agttcccagg aggggagaat ctgcggcagg gcgaccaaga gccctccccc
     1561 gacgcccagg gcccagaagc cggcgggggt ccaacgccgt tcctgggccc cgcgccacag
     1621 gaccgccgct cccgcccaaa gaagcccgta ttcggtgagc
//
LOCUS       DAOQLS010000523         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_55340_length_1659_cov_3.488155, whole genome shotgun sequence.
ACCESSION   DAOQLS010000523 DAOQLS010000000
VERSION     DAOQLS010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55340_length_1659_cov_3.488155"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PK362_09100"
     CDS             <1..438
                     /locus_tag="PK362_09100"
                     /inference="COORDINATES: protein motif:HMM:NF025464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HNC75022.1"
                     /translation="PSDAPENRWWLFGSWHDQPNRDRGETWDGFPSHSPPIAIGYGFL
                     NGVDQLALTYGSPDSTTVGLVPFKRGVWHKVTVRIVWSRGAKGRAELRLDDSPKAVVS
                     ASGPNMHNDFQHYLKVGMYRHREIQGDNWIYLRDIAIKKTGVK"
     gene            624..1061
                     /locus_tag="PK362_09105"
     CDS             624..1061
                     /locus_tag="PK362_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75023.1"
                     /translation="MKAILSFAAESVGLEMEEGLEAVEFENEAEGSSFAFTTETQDKA
                     RKKNPHLVRAWWRHAGQGADEFNMVFTDIRIGLGRFQAQLTVTPSSRAAFPFDGVDIS
                     FAEIDAQETAGLISIFRSMFRDKTDLIALDVPGMPKKLPKLPR"
     gene            1137..1646
                     /locus_tag="PK362_09110"
     CDS             1137..1646
                     /locus_tag="PK362_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75024.1"
                     /translation="MKLTRLRIASQVLFSRWQGVGRAGIIRDLKRRMKKDWNRGVIEL
                     VDYVIKHKKVPDAAPKAAAVREKVLAALAKRLSLKKKGTRWVWTGRFADPASANKKKK
                     EIARFLIRSGFRYARTRGDVGVWTDGYEYRRLRGVDLSAGILLNERRKPHTLTVDVES
                     ARDFIKRIT"
BASE COUNT          364 a          438 c          526 g          331 t
ORIGIN      
        1 ccgtccgacg cgccggagaa ccgttggtgg ttgttcggct cctggcacga ccagcccaat
       61 cgggatcggg gggaaacctg ggacggcttc ccgtcccaca gcccgcccat cgccatcggc
      121 tatggctttt tgaacggcgt ggaccaactc gcgttgacct acggctcgcc ggattcaacc
      181 acggtggggt tggtgccttt caagcgtggg gtgtggcaca aggtgacggt tcgcattgtc
      241 tggtcccggg gggcgaaggg ccgggcggag ttgcgcctgg acgattcgcc caaggctgtt
      301 gtctcggcga gcggccccaa catgcacaac gacttccaac attatttgaa agtggggatg
      361 tatcgacacc gcgaaatcca aggggacaac tggatttacc tgcgggatat cgccatcaag
      421 aaaacgggag taaaatgaat ttaatggatt cgttttccaa tggggcaaac cgggaattcc
      481 gtggttgtta gcgaatttga aaacattggc agaggcgcaa cggcgttggt cgtgattgac
      541 aaggcgtttg tgtggcgagg ggggagagcg gtatccaatg aactctccct ccacaaaccc
      601 caaaaggtga ggccatggaa aaaatgaagg cgatcctctc atttgcggcc gaaagtgtgg
      661 gcctggaaat ggaggagggc ctggaggcgg tcgaatttga aaacgaggcg gaagggtcgt
      721 ccttcgcttt cacgacggag acccaagaca aggcccggaa gaaaaatccg catttggtcc
      781 gcgcctggtg gcgccacgcc gggcaggggg cggacgagtt taatatggtc ttcacggata
      841 tccgaatcgg cctggggcga tttcaagccc aattgaccgt tacaccctcc tcccgggcgg
      901 ccttcccctt tgacggcgtt gatatttcct tcgcggaaat cgacgcccag gaaaccgcgg
      961 gactcatttc gattttccgt tcgatgttcc gggataagac ggatctcatt gccttggatg
     1021 tcccgggcat gccgaagaaa ttgccgaaat tgccgcgatg aggtggaatt ccctttaaat
     1081 ttaccgagga aggcggccga ttgattggaa tttaaaatcc cgattcccaa aaccctatga
     1141 aactcacccg cttacgcatt gcctcacaag tcttgttcag ccgatggcag ggggtgggcc
     1201 gggccggcat catccgcgat ttgaagcggc ggatgaaaaa ggactggaac cgaggcgtca
     1261 tcgagctggt cgattacgtc atcaaacaca agaaagttcc ggacgcggcg ccgaaggcgg
     1321 cggctgttcg ggagaaagtc ttggccgccc tggcaaagcg cttgtcgctc aagaaaaagg
     1381 gcacgcggtg ggtgtggacc ggccgctttg cggacccggc gtcggccaat aagaaaaaga
     1441 aggaaatcgc ccggtttctg attcgctccg ggtttcgtta cgcgcgcacc cggggcgacg
     1501 tgggggtttg gacggacggc tacgaatacc gccggttgcg cggcgtcgac ctgtccgccg
     1561 ggattttgtt gaacgaacgg cgaaagcccc acaccctcac cgtggacgtg gaatcggcca
     1621 gggactttat taagcggatc acctgaaatc cgcggcgcg
//
LOCUS       DAOQLS010000524         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_55349_length_1659_cov_3.117830, whole genome shotgun sequence.
ACCESSION   DAOQLS010000524 DAOQLS010000000
VERSION     DAOQLS010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55349_length_1659_cov_3.117830"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..960)
                     /locus_tag="PK362_09115"
     CDS             complement(43..960)
                     /locus_tag="PK362_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75025.1"
                     /translation="MTPATARLLLVEDDQTLSEIVSTLLKQEAYRVTLTRNGEDALRL
                     AREERPDLILLDVLLPKMSGFEVCQRLRSDPATCLIPIILVTALGATKDKVTGFKLGA
                     DEYLSKPFESMELLARVERVLQRSREELAANPLTGLPGGVALEREIQRRLMDQEAFTV
                     ARADVSGLATFNRLYGYEKGDHVVRLIGMILRSAVVELGNNNDLVAHFGGDDFGFVST
                     SVRAEVVAARALENAETLLLMQYEEKDRTPPPRGPAPALMTLSIGIVDVAPGRFHHHV
                     PVLDEAAAALAEAKKINGHHLIRRDAVPV"
     gene            complement(957..>1659)
                     /locus_tag="PK362_09120"
     CDS             complement(957..>1659)
                     /locus_tag="PK362_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNC75026.1"
                     /translation="IPVNRVKDIMDQLIEKGRVVRGWMGVEILSVNEVIARQFGLKSI
                     EGVMINNVLPGSPADKAGLKRGDVVLSFDGKKTPTQEALVELVGKTPPKKTVAMRIVR
                     DGKERDLTLVTAEMPAETTAAPSAGESRGPGPGEPAEWEGARVVAASSEWAQRYGFAA
                     GVSGVVVLEVQPGGLAERLGLEEGDLVASVNRQKTPDPSAFLKAAKAADAKEGLLLDV
                     NRQGRWVYLSFREGQ"
BASE COUNT          284 a          567 c          499 g          309 t
ORIGIN      
        1 gggattgggc gccgacgcgg tgcgccgggc gcggcggttc gtttaaaccg gaacggcgtc
       61 tcgtcggatc aggtggtggc cgttgatttt cttggcctcg gccagggccg cggcggcttc
      121 gtccaacacg ggcacgtggt ggtgaaagcg cccgggggcc acgtcgacga tcccgatgga
      181 cagcgtcatc aacgccgggg ccgggccccg gggcgggggc gtccggtcct tttcctcgta
      241 ttgcatcagc aaaagcgttt ccgcgttttc cagggcccgg gccgccacga cctccgcccg
      301 caccgacgtg ctcacgaaac cgaaatcgtc cccgccgaag tgggccacca gatcgttgtt
      361 gttgcccaat tccaccaccg cgctgcgcaa aatcatgccg atcaaccgga ccacgtggtc
      421 gcccttctcg tatccgtaca aacggttgaa ggtcgccaat cccgacacgt ccgcccgggc
      481 gacggtgaag gcttcctgat ccatgaggcg tcgctgaatc tcgcgttcca acgccacgcc
      541 cccgggcaat cccgtgaggg ggttggcggc cagctcctcc cgactccgtt gcaacacccg
      601 ctccacccgg gccaaaagct ccatggattc gaagggcttg gacaggtatt cgtcggcgcc
      661 gagcttgaat cccgtgactt tgtccttggt ggcgcccagg gccgtcacga gaataatggg
      721 aatcaggcag gtggccgggt ccgagcgcag tcgttgacac acttcaaagc cgctcatttt
      781 gggaagcagg acatcgagca gaatgaggtc gggccgttcc tcccgggcga gccgaagggc
      841 gtcttccccg tttcgggtca aggtcacccg ataggcctcc tgcttcaaaa gcgtggaaac
      901 gatctcggaa agggtttgat cgtcttcaac gagaagcagg cgcgcggtgg cgggagtcat
      961 tggccttccc ggaagctcaa atacacccac cggccctggc gattcacgtc aagcagcaag
     1021 ccctctttgg catcggcggc cttcgcggct ttgagaaaag ccgacgggtc gggtgttttt
     1081 tggcggttca ccgaggccac caaatcgccc tcttccaacc ccaggcgttc ggccaatccg
     1141 cccggttgca cctccaggac gacgaccccc gacaccccgg cggcgaatcc gtagcgttgg
     1201 gcccattcgg acgaggcggc gacgacgcgc gccccctccc attccgcggg ctccccgggg
     1261 ccgggtccgc ggctttcgcc cgccgacggc gcggcggtcg tttccgccgg catttcggcg
     1321 gtcaccaggg tcaaatcccg ctcttttccg tcccgcacaa tacgcatggc gacggttttt
     1381 ttgggcggcg ttttgcccac caactccacc aacgcctcct gggtgggggt cttcttccca
     1441 tcaaaggaca acaccacgtc gccgcgcttg agacccgcct tgtccgcggg ggaccccgga
     1501 agcacgttgt tgatcatcac gccctcgatc gatttcaacc cgaattgacg cgcgatcacc
     1561 tcgttgaccg acaaaatctc cacgcccatc cacccccgga cgacgcggcc tttctcgatc
     1621 aattggtcca tgatgtcctt cacgcggttc accggaatg
//
LOCUS       DAOQLS010000525         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_55585_length_1655_cov_4.813750, whole genome shotgun sequence.
ACCESSION   DAOQLS010000525 DAOQLS010000000
VERSION     DAOQLS010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55585_length_1655_cov_4.813750"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PK362_09125"
     CDS             <1..853
                     /locus_tag="PK362_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC75027.1"
                     /translation="AIKKLIKEEKATVLLATPTFLQMWLKKFTREDVATLRFALTGAE
                     KLQPGFAKDFQDTLGVPLLEGYGTTELSPAACVSVLDVHHLTEHQIGHKPGKVGRPLP
                     GVSVRIVDPESGAAVPDGRPGLLLVKGPNVMRGYWGLPEKTAEVLRDGWYVTGDIAAV
                     DEDGFVEITDRLSRFSKIGGEMVPHMLVERRLWELSGEPEAQFLVLSAPDEKKGEKLV
                     VLHFNVAQDIDALVAKLAVADMPRLWIPDRRCFYKIDAWPTLASGKVDMVSARKIAQN
                     LLDASPK"
     gene            927..>1655
                     /locus_tag="PK362_09130"
     CDS             927..>1655
                     /locus_tag="PK362_09130"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC75028.1"
                     /translation="MRVTQNIVQRAALAFALGFSASPGRAASVSADFLRLGNGARAAA
                     LGNASTALVNDATAIAWNPAALTRLKTKTATLMHASYLDSAYTYQFAAYGQSVGASLG
                     FGLGVQYLSVGALPQTDLTGTEIGSFSPNDLAVSAGGAWSFGGNSVGLTAKWIRSEIV
                     KSAQTVAGDIGFLSAPLWENRLRVGLVAANLGGGLKYDREKSDLPLVVPAGLDLRTLG
                     LPSTMAGGLPPLAVLPASKVLTRLM"
BASE COUNT          287 a          525 c          539 g          304 t
ORIGIN      
        1 ggccatcaaa aagttgatca aagaggaaaa ggccactgtt cttttggcga cgcccacctt
       61 tctgcagatg tggctcaaaa aattcacccg ggaggacgtg gcgaccctcc ggttcgcctt
      121 gaccggggcg gaaaaactcc agcccggatt tgccaaggat tttcaggaca cgctgggggt
      181 ccccctgttg gaggggtacg gcacgacgga gttgtcgccc gcggcctgcg tgagcgtgct
      241 ggacgtccac cacctcaccg aacaccagat cggccacaag ccgggaaagg tggggcggcc
      301 tctcccgggc gtgtcggtcc ggatcgtcga tcccgagtcc ggcgccgccg ttcccgacgg
      361 ccgcccgggg ttgctcctgg tcaaaggccc caacgtgatg cgggggtact ggggattgcc
      421 cgaaaaaacg gccgaggtgc ttcgggacgg gtggtacgtg accggtgaca tcgccgccgt
      481 ggacgaggac ggttttgtcg aaatcaccga ccggctgtcg cggttttcca agatcggcgg
      541 cgaaatggtt ccccacatgc tggtggagcg ccggctctgg gaattgtccg gcgagcccga
      601 agctcaattc ctcgtgctgt cggcgcccga cgagaaaaaa ggggaaaagc tcgtggtgct
      661 ccatttcaac gtcgcccagg acatcgacgc cctggtggcc aaactggccg tggccgacat
      721 gccccggttg tggattccgg accgccgctg tttctacaaa atcgacgcct ggcccaccct
      781 cgcctccggc aaggtcgaca tggtgtcggc ccggaaaatc gcccaaaacc tgctcgacgc
      841 ttcccccaag taattgcccg cccctcgaaa ggccgccgtc gaacgccgcc ttgtcaaagc
      901 gtcgattcag cgttagactg ggggatatgc gcgtgacgca aaacatcgtt caaagggccg
      961 ccctggcgtt cgccctgggc ttctcggcgt cgccgggccg ggcggcgtcg gtctcggcgg
     1021 atttcctccg tctgggaaac ggcgcccgcg cggcggccct ggggaacgcc tccacggccc
     1081 tcgtcaacga cgccaccgcc atcgcttgga accccgccgc cttgacgcgc ttgaaaacaa
     1141 aaaccgccac cctcatgcac gcgagttatc tggattccgc ctacacgtac caattcgccg
     1201 cctacggcca atcggtgggc gcgtccctcg ggttcggcct cggcgttcag tatttgtcgg
     1261 tgggggcgct gccgcaaacg gatttgacgg gaaccgagat cgggtccttt tcacccaacg
     1321 atctggcggt ttccgccggg ggcgcctggt ccttcggcgg aaactccgtc ggactgacgg
     1381 ccaaatggat tcgaagcgaa atcgtcaagt cggctcagac cgtcgccggg gacatcggct
     1441 tcttgtcggc cccgttgtgg gagaaccgcc tccgcgtggg gctcgtggcg gcgaatctcg
     1501 gcggaggatt gaaatacgat cgggaaaaaa gcgatttgcc cctggtggtc cccgcggggc
     1561 tcgacttgcg gacgttgggc ttgccgtcga ccatggccgg cgggttgccg ccgttggcgg
     1621 tcttgccggc gtcgaaggtg ttgaccaggt tgatg
//
LOCUS       DAOQLS010000526         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_55691_length_1654_cov_3.533458, whole genome shotgun sequence.
ACCESSION   DAOQLS010000526 DAOQLS010000000
VERSION     DAOQLS010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55691_length_1654_cov_3.533458"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PK362_09135"
     CDS             complement(<1..826)
                     /locus_tag="PK362_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNC75029.1"
                     /translation="MSKLFSTFNLGPMSLPNRLVMAPMTRCRAGEGNVPTDLMAEYYA
                     QRASAGLLITEASQVCPEGIGYANTPGIHSEAQVAGWTKVTTAVHRAGGRIVIQLWHV
                     GRISHPLFQPKGALPVAPSAIAPKGTVYTPQGPKPYEIPRALALEELPGIVNAYRQAT
                     LNAKKAGFDGVEIHGANGYLLDQFLRDGSNKRTDRYGGSVENRARLLLEVTDAAIQAW
                     DKDHVGVRLSPGGTFNDMADSDTVGTFSYVIRELDRRGVAFVHLRETTPDDVRHGGA"
     gene            complement(936..>1654)
                     /locus_tag="PK362_09140"
     CDS             complement(936..>1654)
                     /locus_tag="PK362_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNC75030.1"
                     /translation="MRKITTIALALLSTTALWADDAKPWKDQAQLSYLSANGNTKSTT
                     IGASNLFSWTGGHFLLELPAGALNTKSKGERTAEQYYAGEKLQYKITDRNYAFEKFLW
                     ERNTFAGFDHRYDVSGGLGREWWKTVKDNFLTELGGGYINEQRVKTAGRTKFGSGRGY
                     AKYARKLSATADFSQDAEYLHNFKESRDYRVNTETALITSISTNLSLKVSYGWKFRNL
                     PPPTFAKSDSLTAVSLIINY"
BASE COUNT          291 a          476 c          530 g          357 t
ORIGIN      
        1 gggcaccccc gtggcggaca tcgtccggtg tggtttcccg caggtggacg aaggccacgc
       61 cccggcggtc caattcccga atgacatagg aaaaggtccc gacggtgtcg gaatcggcca
      121 tgtcgttgaa ggtgccgccg ggggaaagcc ggacccccac gtggtccttg tcccaggcct
      181 gtatggccgc gtcggtgact tccaaaagca aacgggcccg gttttccacc gatcccccgt
      241 aacgatccgt ccgcttgttg ctgccatccc ggaggaattg gtccagcaaa tacccgttgg
      301 cgccgtggat ttccacgccg tcgaaccccg cttttttggc gttcagggtc gcttgacggt
      361 aggcgttgac aattcccggc agttcttcca gagcaagggc ccgggggatc tcgtagggtt
      421 tgggcccctg gggcgtatac acggtgccct tgggggcgat ggccgacggc gccacgggaa
      481 gggcgccttt cggttggaag agggggtggg aaattcgccc cacgtgccag agttgaatca
      541 cgatccgtcc ccccgcccga tggaccgcgg tggtgacttt ggtccatccg gcgacttggg
      601 cctcgctgtg aatgcccggg gtgttggcgt agccgatccc ttcggggcag acctgggacg
      661 cttccgtgat aaggaggccg gccgaggccc gttgagcgta gtactccgcc ataagatccg
      721 tcgggacgtt tccctctccg gcccggcaac gggtcatggg cgccatcacc aggcggttgg
      781 gcagggacat gggtcccaga ttaaacgttg agaaaagctt ggacatgaaa tcctcctgtg
      841 cgtgtgtcta caagaacgcg aagcggacca aatggttccc gccaaaaaaa aggcccgccc
      901 cggcacaaag gccgggacgg gccttttgaa gcggcttaat agttgatgat cagagaaacg
      961 gccgttaacg aatcgctttt ggcgaaggtc ggcgggggca gattgcggaa cttccagccg
     1021 taggaaacct tcagcgacag attcgtcgat atcgacgtga tcaacgccgt ttcggtgttg
     1081 acccggtaat cccggctttc cttgaagttg tggaggtact cggcgtcctg gctgaaatcc
     1141 gccgtggccg acaacttccg ggcgtatttg gcgtatcccc gacccgaacc gaatttggtt
     1201 cggcccgcgg ttttcacccg ttgttcgttg atgtaaccgc cgccgagttc cgtcaggaag
     1261 ttgtccttga cggttttcca ccattcccgg ccgagaccgc ccgagacgtc gtaacggtgg
     1321 tcgaagcccg cgaaagtgtt tcgttcccac aggaactttt cgaaggcgta attacggtcg
     1381 gtgatcttgt actgcagttt ttcgcccgcg tagtattgct cggcggtccg ttcgcccttg
     1441 cttttcgtgt tcagggctcc ggccggcagt tcgagcagga aatgccctcc ggtccagctg
     1501 aacaaattcg aggcgccgat cgtggtcgat ttcgtgttgc cgttggcgct caagtagctg
     1561 agctgggctt gatctttcca gggcttggcg tcgtcggccc aaagcgccgt cgtggacaga
     1621 agcgcgaggg cgatcgttgt gattttgcgc atgg
//
LOCUS       DAOQLS010000527         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_55696_length_1654_cov_3.234522, whole genome shotgun sequence.
ACCESSION   DAOQLS010000527 DAOQLS010000000
VERSION     DAOQLS010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55696_length_1654_cov_3.234522"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PK362_09145"
     CDS             complement(<1..558)
                     /locus_tag="PK362_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC75031.1"
                     /translation="MTAAKPAAGLRAVILAGGRGTRLQPFTINFPKPLMPLGDKPVLE
                     VLMERLLAHGVTDMTLALGHLAELIKAYFEQKPEWKKKIHLRFVHEDAPLGTAGALSL
                     VPDLKETFLVLNGDLLTDLDLGALVAFHREKGAALTIAAHTREVKIDLGVLDIDGDRR
                     VTGYREKPVSRLEVSMGIYVYEPRVL"
     gene            complement(555..1565)
                     /locus_tag="PK362_09150"
     CDS             complement(555..1565)
                     /locus_tag="PK362_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015611425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC75032.1"
                     /translation="MATRSKSLWSGGPVLVTGAGGFIGSHVVEALLDRGARVRALVHY
                     NGAGRQGFLDEIDGRRRARLEVVAGDVRDAHQMAEVVKGCSAVAHLAALIAIPYSYQA
                     ADSYVATNVQGTLNVLEACRRHKVRRLVVTSTSEVYGTAQRTPMDETHRLNAQSPYAA
                     SKIAADQMALAYHRSHGLPVVVLRPFNTYGPRQSARAVLPTILSQILSGAKTLALGNT
                     APRRDLTFVTDTAAAFVAALERPGLDGEVIHFGQGKAASVGELAEKSMTLLRRRPRLK
                     IDRARARPAASEVDLLLCDAGKARRLLNWTPRVGLDEGLRRTAEYLRRHLGAYRPGDY
                     AR"
BASE COUNT          257 a          549 c          587 g          261 t
ORIGIN      
        1 caaaacgcgg ggctcgtaga cgtaaatccc catgctcact tcgagccggg acacgggttt
       61 ttcccggtag cccgtcaccc ggcggtcgcc gtcgatgtcc aggacgccca gatcgatctt
      121 gacctcccgg gtgtgggccg cgatggtgag ggccgcgccc ttttcccggt gaaaggccac
      181 cagggcgccg aggtccaagt cggtcagcag gtcgccgtta agcaccaaaa aagtttcctt
      241 caggtccggc accagcgaca gggccccggc ggtcccgagg ggcgcgtcct cgtgcacgaa
      301 gcggaggtgg atcttctttt tccattcggg cttctgctcg aaataggcct tgatgagttc
      361 cgccaggtgg ccgagggcca gggtcatgtc ggtcacgccg tgggccagaa gccgttccat
      421 gagcacttcc aggacgggct tgtcgcccag gggcatcagg ggtttgggga aattgatggt
      481 gaagggttga aggcgcgtgc cccggccccc ggccagaatc acggctcgca gtccggcggc
      541 gggtttcgcg gcggtcatcg ggcgtagtcc ccggggcggt aggcgccgag atgccggcga
      601 aggtactcgg cggtgcggcg aaggccctcg tccaatccca cccggggcgt ccagttcaag
      661 agccgacggg ccttgcccgc gtcgcagagc aacaaatcca cttcgctggc ggcgggtcgg
      721 gcccgggcgc ggtcgatttt aaggcgcggc cggcggcgga ggagcgtcat gcttttttcc
      781 gccagctcgc ccacgctggc ggccttcccc tggccgaaat ggatcacctc gccgtccaag
      841 cccgggcgct ccagggccgc gacgaaggcg gccgccgtgt ccgtcacgaa cgtcaggtcc
      901 cggcgggggg cggtgttccc cagagcgagg gtcttggccc ccgaaagaat ttgggaaagg
      961 atcgtcggca acacggcccg ggccgactga cgcggcccgt aggtgttgaa ggggcggagc
     1021 accacgacgg gcaggccgtg gcttcggtgg taggccaggg ccatttggtc ggcggcgatt
     1081 ttgctggccg cgtagggcga ctgggcgttc aaccggtggg tttcgtccat gggcgtccgc
     1141 tgggccgtgc cgtagacttc gctggtggag gtcacgacca accggcgcac tttgtggcgg
     1201 cggcaggctt ccaacacgtt cagcgtgccc tggacgttgg tggccacgta ggagtcggcc
     1261 gcttgatagg aatagggaat ggcgatcagg gccgccaggt gggccaccgc cgaacagccc
     1321 ttcacgacct cggccatctg gtgggcgtcc cgcacgtccc cggccacgac ctccaaccgg
     1381 gcgcgccgtc ggccgtcgat ctcgtccaaa aagccttggc gccccgcgcc gttgtaatgg
     1441 accagcgccc gcacccgggc gccccggtcc aagagggcct ccaccacgtg gctgccgatg
     1501 aaaccgcccg cgccggtcac cagcaccggc ccgccggacc aaagggattt tgaacgcgtc
     1561 gccacggtca gccctcccgc cgggcggcga agacccgggc cgccggaatc catccggtga
     1621 aaatccggta ggcgcggacc gcgccgcgcc gggc
//
LOCUS       DAOQLS010000528         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_56006_length_1649_cov_4.730238, whole genome shotgun sequence.
ACCESSION   DAOQLS010000528 DAOQLS010000000
VERSION     DAOQLS010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56006_length_1649_cov_4.730238"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1649
                     /locus_tag="PK362_09155"
     CDS             17..>1649
                     /locus_tag="PK362_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC75033.1"
                     /translation="MVATANIVSFLGIGGASLVYFLLTDPLALGYSPARAWFLVALGA
                     LGATVYVFSLLAPAVIRFAAWVLTHTGYRVTTLGAENIPARGGALLVSNHLSYADPFL
                     MTAAVPRRVRFMMMRPLYNYRPLTWFFKMMGAIPIAGNDGPKKFMASIEQARQALREG
                     HIVCIFAEGAISRLAQLLGFRKGLEAIAKDLDVPIVPVHLDRVWGSVFSFERGKFIWK
                     MPRQIPYPVTVSFGTALPSSTRADRVRQDVLELASRAFEQRLAGLRPLHQYFFRIARR
                     QWFHPAVSDSTGLRLGYGRLVTAAAFLAGKLDVLLPSEKNVGILLPPGVPGLLTNLAL
                     LFAGRVPVNLNYSLGRPVVDQICRDAGVSRVITARKMLEALKWGEEPRAVYLEDLPRP
                     SKLRGLWMFAGLRALPARWAERWWLSKSDATMDDVAALLFTSGSTGQPKGVTLTHKNV
                     HANIQGLQETFQLTPRDKIVGVLPFFHSFGFTGTMWLPILAGAQAAYHRSPLEPLAIK
                     KLIKEEKATVLLATPTFLQMWLKKFTREDVATLRFA"
BASE COUNT          255 a          554 c          503 g          337 t
ORIGIN      
        1 gggccggcgc ggaaccatgg tggccacggc caacatcgtg agctttctgg ggatcggcgg
       61 cgcctccctg gtctactttc ttctaacgga tcccctcgcc ctgggctatt ccccggcccg
      121 ggcctggttc ctggtggcgc tgggcgccct gggcgccacc gtctatgtct tttccctgtt
      181 ggcgccggcc gtgattcgtt tcgcggcctg ggtgctgacc cacacggggt accgggtcac
      241 gaccctcggg gccgaaaaca tccccgcccg gggcggggcc cttctggtgt ccaatcactt
      301 gtcctacgcc gatccgtttt tgatgacggc ggccgtgccg cgtcgggtgc gttttatgat
      361 gatgcgcccc ctctacaact accggcccct cacctggttt ttcaaaatga tgggggccat
      421 ccccatcgcc ggaaacgacg gccccaaaaa gttcatggcg agcatcgagc aggcccggca
      481 ggccctgcgg gaggggcaca tcgtctgtat cttcgccgaa ggggccattt cgcgcctggc
      541 ccaactcctc ggattccgca aagggttgga ggccatcgcc aaggacttgg acgttcccat
      601 cgttcccgtc catttggacc gcgtgtgggg gagcgtgttc agtttcgagc ggggaaaatt
      661 tatttggaaa atgccccggc aaatccccta ccccgtgacc gtgtccttcg ggacggcctt
      721 gccctcctcg actcgggcgg accgcgtccg gcaggacgtg ttggaattgg cgagccgcgc
      781 ttttgaacag cgcctggcgg gcctgcgccc gctgcatcaa tattttttcc ggatcgcccg
      841 ccgccaatgg ttccaccccg ccgtgtccga ttccaccggc ttgcgcctgg ggtacgggcg
      901 ccttgtgacg gcggccgcct tcctggccgg gaagttggac gtgttgcttc cgtccgagaa
      961 aaacgtggga atccttctgc ccccgggcgt tcccggccta ttgaccaacc tagcgctcct
     1021 cttcgccggc cgggtgcccg tgaacttgaa ctactccctg ggccgccccg tcgtcgacca
     1081 aatttgccgg gacgccgggg tctcccgggt gatcaccgcc cggaaaatgc tcgaggcgct
     1141 caagtggggg gaggagcccc gcgccgttta tctggaagac cttccgcgcc cctcgaaact
     1201 tcggggtctc tggatgttcg cgggccttcg cgccttgccc gcccggtggg cggagcggtg
     1261 gtggctttcc aaatccgacg cgaccatgga cgacgtggcc gccctgcttt tcaccagcgg
     1321 cagcacgggc cagcccaagg gcgtcaccct gacgcacaag aacgtccacg ccaacatcca
     1381 aggacttcaa gagactttcc agctgacgcc gagggacaag atcgtcggcg ttctgccgtt
     1441 tttccattcc ttcggattca cgggtacgat gtggctgccc atcctggccg gggcccaggc
     1501 cgcctaccat cgaagtcccc tggaacctct ggccatcaaa aagttgatca aagaggaaaa
     1561 ggccaccgtt cttttggcga cgccgacctt tctgcagatg tggctcaaga aattcacccg
     1621 ggaggacgtg gcgactctcc ggttcgcct
//
LOCUS       DAOQLS010000529         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_56173_length_1646_cov_4.248272, whole genome shotgun sequence.
ACCESSION   DAOQLS010000529 DAOQLS010000000
VERSION     DAOQLS010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56173_length_1646_cov_4.248272"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1646
                     /locus_tag="PK362_09160"
     CDS             <1..>1646
                     /locus_tag="PK362_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75034.1"
                     /translation="SSERRDKIDAVLHMDRRTDTHDFVSGTVYIRGEQIAFQGGKLEV
                     VNAKAGSTIMGYTTLTDVASFTLSAKGEIGGLGIGERVEHASGLVFGGVEAASRTTAR
                     AASAAADKAEVRVDANGNISLTAPTVQITATRVVTVPVAVADRLLPAGESGEIVGMMN
                     SDKGFAYRTAGNMWFVMSEGKLQAGSGTLTTTSEVANLAGGKVTITNRVVLTSDGHQK
                     ATEMLINGSSVKGGYTLANGNTAYLLSNGKLFEVAYDKGVAVTARSVNTTERLALPDF
                     RDGTTTLSRSLGYDEKTNRWQANFTSLTTVVGGRTKTMDIVGAFSDPKSQATLLLVAD
                     RSNGQRGREYLFALKQDSEGAITGVTAFGNKSIDFAPEKGKPLGQYLKTNAQGQITER
                     GTTIMIDGKLVRNNSVMDMSNARQQLGLLQDMKGYFAVQNGRLITWDEGKKQWVRVNG
                     GVQEFSNQKMYIVTAKDGAAPTAAGLQARQFNATAVGVILSLRVQDGKLQFDNRIDPK
                     TADASKMFWEARRLDGDGAALSFKDISSAAFKSAVAWVGT"
BASE COUNT          398 a          459 c          481 g          308 t
ORIGIN      
        1 gttcctcgga acgccgggac aagatcgacg ccgttctcca catggaccgg cggaccgaca
       61 cccacgattt cgtctccggg acggtttaca ttcgcggcga gcaaatcgcc ttccagggcg
      121 gcaaactgga agtcgtcaac gccaaggcgg gcagcaccat catgggttac acgaccctga
      181 ccgacgtcgc ctcttttacc ctgagcgcca aaggcgaaat cggaggtttg gggatcggcg
      241 aacgggtgga acacgccagc ggattggttt tcggcggcgt tgaagccgcc agcagaacga
      301 ccgcccgcgc cgcctcggcg gccgcggata aagccgaggt gcgggtggac gccaacggga
      361 acatcagtct gacggccccg accgttcaga tcaccgccac gcgcgtggtc accgtgcccg
      421 tggccgtcgc cgatcgcctt cttccggcag gcgaaagcgg cgaaattgtc ggaatgatga
      481 attccgataa aggcttcgcc tatcgaacgg ccgggaatat gtggtttgtg atgtccgaag
      541 gcaagcttca agcgggatcc ggaacactta cgaccacgtc cgaagtcgcc aacctcgccg
      601 gcgggaaagt cacgattacc aatcgggtcg tcctaacctc cgatggtcat caaaaggcaa
      661 cggaaatgtt gataaatggg tcgagcgtca aaggcgggta tacgctggca aatggaaaca
      721 ccgcctatct attatcgaac gggaaacttt ttgaggtggc gtacgacaag ggagtggccg
      781 tcaccgcgcg atccgtcaat acaacggaac gcctggcgtt gcccgatttc cgggatggaa
      841 cgacgacgct ttcccgatcc ttggggtatg acgagaaaac caatcgttgg caggccaatt
      901 tcacctccct gacaaccgtg gttggaggtc ggaccaaaac gatggacatt gtcggtgcct
      961 tcagcgaccc caaatcccag gccacactgc ttttggtggc cgatcgatcc aatgggcaac
     1021 gcggacggga atacctcttt gcacttaaac aagattccga aggagccatc acgggcgtta
     1081 cggcctttgg gaacaaatcg attgatttcg caccggaaaa gggaaaaccg ctgggtcaat
     1141 atttaaaaac aaacgcccaa ggccagatca ccgaacgcgg gaccaccatt atgatcgatg
     1201 ggaagctggt tcgaaacaac agcgtcatgg atatgtccaa cgctcgtcaa cagcttgggc
     1261 ttctccagga catgaaaggg tatttcgcgg tacaaaacgg tcgtctgatt acatgggacg
     1321 aggggaaaaa gcaatgggtt cgcgttaacg ggggcgttca agagttcagc aatcaaaaaa
     1381 tgtatatcgt gacggccaaa gacggggcgg ctcccaccgc cgccggattg caggctcgcc
     1441 aatttaacgc gaccgccgtc ggggtcatac tttccctgcg cgttcaagac ggcaaattgc
     1501 aattcgacaa ccgcattgat cccaaaaccg ccgacgccag caaaatgttt tgggaagccc
     1561 gtcgactgga cggggatgga gcggcgttat cgttcaagga tatttcttcc gccgccttta
     1621 aatcggccgt tgcctgggtg gggacc
//
LOCUS       DAOQLS010000530         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_56175_length_1646_cov_4.185418, whole genome shotgun sequence.
ACCESSION   DAOQLS010000530 DAOQLS010000000
VERSION     DAOQLS010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56175_length_1646_cov_4.185418"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..>1646)
                     /locus_tag="PK362_09165"
     CDS             complement(24..>1646)
                     /locus_tag="PK362_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75035.1"
                     /translation="YWTKKRLSVSNARFAAGHDPVFYWPYYSKSLKDNRWTLTVDPGN
                     SARNGYFAKSVFTYPISDETRASVLWDYYSKSGNGYGGEFAYNHPKVRGSVSGYTIED
                     RLTNGRRWNLRVGHWQQIAQRWSIQSNVAFQSDREVNNTFVRDDYDRVRQLGESSLAL
                     TRSGAIFTSRISVEHDRSFDADQDRFVTQRTILPQLSVQSSPIRLGRSNFYVNTSANF
                     RNDYVRPSLSGLTPNPINPAKDTYRQYGDGTLGIKWRLPLTKSLTLEPSAGVTEQWQS
                     HTDSGTVLDPADVTQGRGFTNLNWRHRLTRSLDYDLSHRYKVRWSPNTFHRDTLAADR
                     GLEQNDLSFFGSYRAGSTFWARATTAYDLRDTPNLNYQSPRQRFTPPALEVSAQPRPW
                     IKAFLRETVRLYPVRQPQTTAATLRFGSDDKTYLSNGISYNVGRPGQLDISQGAAFNL
                     TRGWWLSGDLQYTASGQGRLRYNKIDIREKNLIVRRDLHCWVVRITYRERPGVNEVFL
                     RVDLKTNLELRQRTALPDEQQFHPARDTRDAY"
BASE COUNT          300 a          463 c          548 g          335 t
ORIGIN      
        1 caccagaatt ttcattcgcc ctctcagtac gcgtcccggg tgtcccgggc gggatggaat
       61 tgctgctcgt cgggcagggc cgtccgctgg cgcaattcca aattcgtttt caaatcgacc
      121 cgaaggaaca cttcgttgac gcccgggcgc tcgcggtagg tgatccgcac cacccaacaa
      181 tggaggtccc gtcgcacgat caggtttttt tcacgaatat cgattttgtt gtagcggaga
      241 cgcccttggc ccgaggcggt gtattggagg tcgccggaca accaccagcc ccgggtcaaa
      301 ttgaaggcgg ccccctggga aatgtccagt tgccccggac ggccgacatt gtagctaatt
      361 ccgttggaaa gataggtttt gtcgtcggaa ccgaaccgca gcgtggccgc cgtggtttgg
      421 ggttggcgca cgggatagag acggacggtc tcgcgaagaa acgctttgat ccaaggccgg
      481 ggctgggcgc tcacctccag ggccgggggg gtgaagcgtt gccggggcga ttggtagttc
      541 aggtttggtg tgtcccgcag atcataggcc gtggtggccc gggcccaaaa agtcgacccg
      601 gcacggtagg agccgaaaaa cgataaatcg ttttgctcga ggccccgatc ggccgccaag
      661 gtgtcccggt ggaaggtgtt ggggctccag cggactttgt agcggtggga caggtcgtaa
      721 tccaaggaac gggtcagccg gtgtcgccag ttcaggttgg tgaagccccg cccttgggtc
      781 acgtccgcgg gatccagaac cgtccccgaa tcggtgtggg attgccactg ttccgtcacc
      841 cccgcggaag gttccagggt cagcgacttc gtgagcggca ggcgccattt gatccccagg
      901 gtgccgtccc cgtattggcg gtacgtgtcc ttggccgggt tgatggggtt gggcgtcaga
      961 cccgacaggg atggacggac gtaatcgttc cggaaattgg ccgaggtgtt gacgtagaaa
     1021 ttgctgcgtc ccaggcgaat ggggctcgac tgaacgctga gctggggcag gatcgtgcgc
     1081 tgggtgacga accggtcctg atccgcgtcg aaagaacgat cgtgctccac ggaaatccgc
     1141 gaggtgaaaa tggccccgct tcgggtgagg gccaggctgg attcccccag ctggcggacc
     1201 cggtcgtagt cgtcccgcac gaaggtgttg ttgacctccc ggtcgctttg aaacgccacg
     1261 ttggactgaa tcgaccaccg ttgggcgatc tgctgccaat gcccgacccg caggttccac
     1321 cggcggccgt tggtcaggcg gtcttcgatg gtgtagccgg aaacggatcc ccggactttc
     1381 gggtggttgt aggcgaattc cccgccgtat ccgtttccgg atttcgaata gtaatcccaa
     1441 aggacgctgg cccgggtttc gtccgaaatc gggtaggtga acaccgattt ggcgaaatag
     1501 ccgttccggg cgctgttgcc cggatccacc gtcaacgtcc agcggttgtc cttcagagac
     1561 ttgctgtagt agggccaata aaacaccgga tcgtggcccg ccgcgaagcg ggcgttcgaa
     1621 accgacaagc gtttcttcgt ccagta
//
LOCUS       DAOQLS010000531         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_56342_length_1644_cov_2.594084, whole genome shotgun sequence.
ACCESSION   DAOQLS010000531 DAOQLS010000000
VERSION     DAOQLS010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56342_length_1644_cov_2.594084"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1644)
                     /locus_tag="PK362_09170"
     CDS             complement(<1..>1644)
                     /locus_tag="PK362_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC75036.1"
                     /translation="NLWTAVATVLFVLGTLEFHATPASLLLGWFPNSTFAFSSGLPVL
                     AGVFGAAAVGIGAWKTRLRWHPFLGLLGATVLASAGIFVGDLTVLVLDAAGSALGFRP
                     WGSALGVLPLAVDASPVTLLNAASYVFFSAAFMLGLRKAQGRLLWDRFLGRVLVVVDP
                     LHANARLSAARWRRMLNLRFGWMGLYSVNESSLGRMTHEEAPNTNVRGTLYLQNESRV
                     SESATAMNFKQLGGSPNGPGRAWRMGIGHKPAGTANAAFTEGYVSLAMADDNPAGTDA
                     DLLTLQEYFQDAGARDTAGMALALNVAEGLANRRPLNFVIGQTSSEAKTSTTQQPHPP
                     LPAGLVRDVFGVRTPPAPTPVQSARPRGGNGRNSRSGTPGAKVEPSTPVSDPKPAGET
                     TDRAGTTHVAPPTEAPGAGSVEARPPLWRRWASSAQRTLRRALRFAALGLLLAVFVTL
                     PGSQNRWPDVHSAVPVPLRLSEHLSPPADIGGLLKRSEAGRVVSRYGGPVLLREAAGS
                     RSKILTVIPAGLSVAVLAKEGRWARVQVGAVTGYVQSAYV"
BASE COUNT          311 a          566 c          534 g          233 t
ORIGIN      
        1 acgtaggccg attgaacata cccggtcacc gcgccgacct ggacccgggc ccagcgtcct
       61 tccttcgcca atacggcgac gctgagccca gcggggataa cggtgaggat ttttgagcgc
      121 gaacccgcgg cctcccggag gaggaccggc cccccgtaac gggacaccac gcgtcccgct
      181 tcgctccgct tcaacaaacc tccgatgtcc gccggcgggg acaaatgttc ggagagccgc
      241 aacggcacgg gcaccgccga atgcacgtcg ggccaacggt tttgggagcc cggcaacgtg
      301 acgaaaaccg ccaacaacag acccagggcg gcgaaccgca gggcccgccg caacgtccgc
      361 tgggcgctcg acgcccaacg acgccacaag ggcggtcgcg cttcgacgga tccggccccg
      421 ggcgcctcgg tgggcggggc aacgtgagtc gtcccggccc ggtccgtcgt ttcccccgcc
      481 ggtttcggat ccgacaccgg cgtcgatggt tccactttcg ctcccggggt cccggacctt
      541 gaattccggc cgttccctcc ccggggacga gccgattgaa cgggcgttgg ggccgggggc
      601 gtccgaaccc cgaaaacgtc ccgaacgagg cccgcgggca gcggcgggtg gggctgctgg
      661 gtggtcgacg ttttggcttc gctggaggtt tggccgatca caaaattcag gggtcgccgg
      721 ttggccagtc cctcggcgac gtttaaggcc agggccatgc cggccgtgtc ccgggcgccg
      781 gcgtcctgga aatactcctg gagggtcagg aggtcggcgt cggtccccgc cggattgtcg
      841 tcggccatgg ccaaggacac atagccttcc gtgaaggcgg cgttcgccgt ccccgccggc
      901 ttgtgcccga tgcccatccg ccaggcgcgg ccggggccgt tggggcttcc gccgagttgt
      961 ttgaaattca tggccgtggc gctctcgctg acccgggatt cgttttgaag gtaaagggtt
     1021 ccccgcacgt tggtgttcgg ggcctcctcg tgcgtcattc gaccgagcga cgattcgttg
     1081 acggaataga gccccatcca tccgaaacgg agattcagca tccgacgcca acgggccgcg
     1141 ctcaaccggg cgttggcgtg caggggatcc accaccacaa ggacgcgtcc caggaaacgg
     1201 tcccacaaca gccgcccctg cgctttacgc aaccccaaca tgaaggcggc ggagaaaaaa
     1261 acataactcg ccgcgttgag aagcgtgacc ggggaggcgt cgacggccaa cggaagcacg
     1321 cccagagcgg aaccccacgg ccgaaacccc agggccgagc ccgcggcgtc caacaccaac
     1381 acggttaaat ccccgacgaa aatgcccgcg ctggcgagca cggtcgcccc cagcaggccg
     1441 agaaaagggt gccagcgaag gcgcgttttc cacgcgccga ttccgaccgc cgccgcccca
     1501 aacacccccg cgagcacggg caatccggac gaaaaagcga aagtcgaatt gggaaaccac
     1561 cccaacaaca aactggccgg ggtggcgtgg aattccaagg ttcccaggac gaacaaaacc
     1621 gtggccaccg ccgtccagag gttt
//
LOCUS       DAOQLS010000532         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_56590_length_1640_cov_4.459306, whole genome shotgun sequence.
ACCESSION   DAOQLS010000532 DAOQLS010000000
VERSION     DAOQLS010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56590_length_1640_cov_4.459306"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PK362_09175"
     CDS             <1..831
                     /locus_tag="PK362_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75037.1"
                     /translation="VRDLLLNRPPKDWDVVVEGAAGPVVRDVAAAEDARVESHPAFLT
                     FAIHFKDGTALDVATARTETYARPGALPTVRPAALAEDFARRDFTVNAMACRATPADR
                     GRLEDPLGGRADLDRGLVRALHPKSFEDDPTRLYRAARYAARYGWSVEPDTAGWIDRA
                     VREDRPALLSPVRRRNELFHLLEEDRPLAALQLLETWGLGRFAVPSYDLNRVPASVWV
                     GEPSADRLARRLAGLLGPDPAVAEKALRAAQTPVPLRRRVTEWLRDPRFVVDTFGRKN
                     "
     gene            865..>1640
                     /gene="trpS"
                     /locus_tag="PK362_09180"
     CDS             865..>1640
                     /gene="trpS"
                     /locus_tag="PK362_09180"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNC75038.1"
                     /translation="MTPSSTVILSGMRPTGKLHLGNYWGALSPWLDLQKDPAKKCFFM
                     VADLHALTTGYEETAGIKGNIDDMVLDWLAAGLDPSKSVLFIQSKVPEHAELALLLSM
                     ITPLGWLERIPTYKDQLRELAGREIATHGFLGYPVLQSADILIYKATAVPVGEDQLSH
                     LELCQEIARRFNRLFGPVFPEPKAILGRTPKVPGLDGRKMSKSYGNAVELSDTPDILW
                     SKIQKMYTDPKKLRANDPGHPDGCVVFALHKIYNPTVEERA"
BASE COUNT          277 a          552 c          513 g          298 t
ORIGIN      
        1 gtccgggacc ttttgttgaa ccggcccccg aaagattggg acgtcgtggt ggagggggcc
       61 gcgggcccgg tcgtgcggga cgtcgccgcc gccgaggacg cccgggtgga gtctcatccg
      121 gcctttctca ccttcgccat tcactttaaa gacggaaccg ccctggacgt cgccaccgcg
      181 cgcaccgaaa cctacgcccg gcccggcgcc ctgccgaccg tccgtccggc cgccctggcg
      241 gaggatttcg cccggcggga ttttaccgtc aacgccatgg cctgccgggc caccccggcg
      301 gaccggggcc gcctcgagga ccccttgggc gggcgcgcgg acctcgaccg gggcctggtg
      361 cgggcccttc acccaaaaag ttttgaggac gatcccacgc gcctttaccg ggcggcccgc
      421 tacgcggccc ggtacgggtg gtccgttgag ccggacaccg ccgggtggat cgaccgggcc
      481 gttcgggagg accgtccggc cttgctgagc cccgtccgcc gccgaaacga acttttccat
      541 ttgttggagg aggaccggcc tttggccgcc cttcaattat tggagacctg gggcctggga
      601 cgttttgccg tgccgtccta cgatttaaac cgagttccgg cgtcggtctg ggtcggggag
      661 ccgtccgcgg atcgcctggc ccgccgcctg gcgggcctcc tgggacccga cccggcggtg
      721 gcggaaaaag cccttcgggc ggcccaaacc ccggtgcctc tgcggcgccg ggtgaccgag
      781 tggcttcggg atccccggtt cgtcgttgac accttcgggc ggaaaaatta atatgccccc
      841 gcccacgtcg gccccccttc gcccatgacc ccttcctcca ccgtcattct gtccggcatg
      901 cgtcccaccg gaaaattgca tttgggcaac tattggggcg ccctgtcccc ctggctggac
      961 ctgcaaaaag accccgccaa aaaatgtttt ttcatggtgg ccgatctcca cgccctgacg
     1021 accggctacg aggagaccgc gggcatcaag ggcaacatcg acgacatggt gctggactgg
     1081 ctggcggcgg ggctggaccc gtccaaaagc gtgctcttca tccaatccaa agtgccggaa
     1141 cacgcggaat tggccctgct gttgtccatg atcacgcccc tgggctggct cgagcgcatt
     1201 ccgacctata aagaccagtt gcgggaactg gcgggccgcg aaatcgcgac ccacggattt
     1261 ttggggtatc cggtcctcca atccgcggac atcttgattt acaaggcgac ggccgtcccg
     1321 gtgggggaag accagttgtc ccatttggaa ctgtgccagg agatcgcgcg gcggttcaac
     1381 cgcctgttcg gcccggtttt tccggaaccc aaggccatcc tgggccggac ccccaaggtc
     1441 cccgggttgg atggccgcaa aatgtcgaaa tcctacggca atgcggtgga actgtcggac
     1501 acgccggata ttttatggtc caagattcaa aaaatgtaca cggacccgaa aaaattgcgg
     1561 gccaacgacc ccggccatcc ggacggctgc gttgtgttcg cccttcataa aatttataat
     1621 cccaccgtcg aggaacgcgc
//
LOCUS       DAOQLS010000533         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57048_length_1633_cov_5.425856, whole genome shotgun sequence.
ACCESSION   DAOQLS010000533 DAOQLS010000000
VERSION     DAOQLS010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57048_length_1633_cov_5.425856"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..>1633)
                     /locus_tag="PK362_09185"
     CDS             complement(140..>1633)
                     /locus_tag="PK362_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNC75039.1"
                     /translation="LTVALFRFDGSTTTGLVSGTTGDFYVVPLDPSLQPLDVTNLVNP
                     TPTYPAEPNKFQMDPSGVVTLTLSYRTAENVIFRIYDADGWQGYSNNRVPIAFVPRDV
                     TYSVTQPTESTVGPPSAFSMVIVSSDADTGTVAKNFSSTFTITPIRVVDGLPGAGVLQ
                     VTNGSMAGGPVTLQQAYTEAGLFRFRIDDGTRFSDSPIINMKPGSLASLSLALPASLE
                     AGTTAQIQVTLLDGFSNPIPNLPTILSLDNSALGTLLNPSGLSNAFGLLNVDFQASAG
                     GSGPGTFIARSGSFSASQGFRLLGPPVTSLSFSGYGQSTGKGSALKTGDLVTINAAYE
                     AGMALTEVRYSVDGGPFLVYGGPFALPGIGVHSVRHYGVTQAGFTHVESTRTSPTIYV
                     SAATSSDEGLVNYPNPFQAGRDVTFLEYNLAQASNVTLTIYDVMGQKIYETEVSAGQT
                     GGQAGLNRMAWDGRNADGETVGNGGYIAVLELKADGKTFRRKVAVRK"
BASE COUNT          296 a          501 c          555 g          281 t
ORIGIN      
        1 agggcgcggg cgcccgcccc gaaccgcaaa tagtccccgg cgtcgccgaa ggtgacttcg
       61 gcccgggccg ccggagcggc ggccgccaaa aggaccgcca cgggggcgtt gaccaggctt
      121 cgccgaagtc ctcggaaatt catttacgca ccgccacctt tcgccggaac gtttttccgt
      181 cggctttcag ttccagcacc gcgatgtagc cgccgttgcc gaccgtttcg ccgtccgcgt
      241 tgcggccgtc ccaggccatc cggttcaacc cggcctggcc gccggtctgg cccgcggaca
      301 cctcggtttc gtagatcttt tggcccatca cgtcgtaaat ggtcaaggtc acgttggagg
      361 cctgggcgag gttgtattcc aggaacgtca cgtcccgccc ggcttggaag gggttcgggt
      421 agttgaccag gccctcgtcc gaggaggtcg cggcggacac gtatattgtc gggcttgtcc
      481 gggtcgattc cacgtgggtg aacccggcct gggtgacgcc gtagtggcgc accgaatgaa
      541 cgccgatccc cggcaaggcg aagggcccgc cgtacaccag gaacggaccg ccgtccacgg
      601 aataccgaac ctcggtcaag gccatgcccg cctcgtaggc ggcgttgatc gtcaccaggt
      661 ccccggtttt caaggcgctt cccttgccgg tggattggcc gtacccggag aaactcaagg
      721 aggtgacggg cggtcccagg agccggaagc cctggctggc gctaaaggac ccggaccgcg
      781 cgataaaggt gcccgggccc gacccgcccg cggaagcttg aaaatcaacg ttcagaaggc
      841 caaacgcgtt gctcagtccc gaggggttca acagcgtgcc cagggcgctg ttgtccagcg
      901 acaggatcgt gggcagattc ggaatcggat tcgagaatcc gtccaagagc gtcacttgga
      961 tttgggcggt cgtcccggct tccagggaag cgggcaacgc cagggaaagg ctggccaggg
     1021 accccggctt catgttgatg atcgggctgt cggaaaaccg cgttccatcg tcgatccgga
     1081 accggaacaa cccggcttcc gtgtaggctt gctggagcgt tacgggcccg cccgccatgg
     1141 atccgttggt gacctgcagg acgcccgccc ccggcaatcc gtccacgacc cgaatgggcg
     1201 tgatggtgaa ggtggaggaa aaattcttgg cgaccgtgcc ggtgtccgcg tcggaggaca
     1261 cgatgaccat ggagaaggcc gagggggggc cgacggtcga ttcggtcggc tgggtcacgc
     1321 tgtaggtgac gtcccgcggc acgaaagcga tgggcacccg gttgttgctg tagccttgcc
     1381 agccgtcggc gtcgtaaatc cggaagatga cgttttccgc ggtgcgataa gacagcgtga
     1441 gcgtcaccac cccggaggga tccatttgga atttattggg ttcggccgga taggtcgggg
     1501 tcggattcac caaattcgtc acatccaggg gctgcaggga cgggtccagg ggcacgacgt
     1561 aaaagtcccc ggtggtgccg gacaccaaac ccgtcgtggt cgaaccgtcg aaacggaaga
     1621 gggccacggt cag
//
LOCUS       DAOQLS010000534         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57117_length_1632_cov_4.549144, whole genome shotgun sequence.
ACCESSION   DAOQLS010000534 DAOQLS010000000
VERSION     DAOQLS010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57117_length_1632_cov_4.549144"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1031)
                     /locus_tag="PK362_09190"
     CDS             complement(105..1031)
                     /locus_tag="PK362_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75040.1"
                     /translation="MKFHLHRVWKPAATAMLALVAGSAEAAFRDPLWSARAAALGGAF
                     TALGDDPTAAFYNPAATTELKSPAANFTYAKLFAGLDEVNVSLSEFAYVRPLKSFNVL
                     SVGWGSVNASGLRREDTVTLGVAHLFRDLGDLGNLSLGVSGRWLSQSFTLDNRTSSDP
                     VFRDGRTRNDFALDLHAHVTEVDALSRNLALGVSLRAVNRPDIGFASKDKLPMEAVLG
                     LAYRWRNCTLPLDIVQRGSDVTPEAGLEARFAADHLSLRFGSDTHSLGTGLGYRYERP
                     NGLVLSFDYAFLWPLNIQGTSGSHRATLGVRF"
     gene            complement(1033..>1632)
                     /locus_tag="PK362_09195"
     CDS             complement(1033..>1632)
                     /locus_tag="PK362_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNC75041.1"
                     /translation="PRSLNGDQTIQVTLKPSTHYPPVEGGVPLRVFDLTPEGLRFSRP
                     VTLSIPYPDQDQDGRVDGSGVDETLLRVFWYDGHLWRFVGGQVDPVANRVSVTVSHFS
                     VYSLAPLATTLTPDMVRPQEKIITPNGDGQNDFAQFNISGEFDVEFFDLRGERVRRLS
                     GINSWDGRDDDGKLVETGTYIYRLTGQGLTVTGALAVAR"
BASE COUNT          313 a          525 c          532 g          262 t
ORIGIN      
        1 atcgtttcag gtttgacgtc acggcgtttt ttggcggcct cacgttggat cgccgagaag
       61 gatcgacccg atcgccgccc cccagagcgc ccctcgttga acgttcaaaa ccgcaccccc
      121 agggtggccc ggtgagaccc ggaggtccct tgaatgttga ggggccaaag gaaggcgtaa
      181 tcaaaagaca gcacgagccc gttgggacgt tcgtagcggt agcccaggcc ggtgccgagc
      241 gagtgcgtgt cgctcccgaa ccggagggac aggtggtcgg cggcaaaccg ggcctccaag
      301 cccgcctcgg gggtgacgtc ggaaccccgc tgaacgatgt ccaagggcaa cgtgcaattc
      361 cgccaccggt aggcgaggcc caataccgcc tccatgggca atttgtcttt ggaggcgaac
      421 ccgatgtcgg gacggttgac cgcccgcagg gaaacgccca gggcgaggtt ccgggagagg
      481 gcgtccacct cggtcacgtg ggcgtgaagg tccaaggcga agtcgttgcg cgtgcgcccg
      541 tcccggaaaa cggggtcgct ggaggtacgg ttatcgagcg tgaacgactg ggacaaccac
      601 cgacccgaaa caccgagaga caggtttccg aggtcgccca aatcgcgaaa cagatgggcc
      661 acgccgaggg tcacggtgtc ttcccggcgc aaaccggagg cgttgacgct cccccacccg
      721 accgacagga cgttaaagga tttgagcggc cgcacatagg cgaactcgct taaggacaca
      781 ttcacctcgt ccagcccggc gaacagtttg gcgtaggtga aattggccgc cggcgatttc
      841 aattccgtcg tcgccgcggg attgtaaaaa gccgccgtgg gatcgtcccc cagcgccgtg
      901 aaggcgccgc ccagggccgc cgcccgggcc gaccaaaggg gatcccggaa agccgcctcg
      961 gcggacccgg cgaccaacgc caacatcgcc gtcgcggccg gcttccaaac tcgatggaga
     1021 tggaatttca tatcaacgcg ccaccgccag ggcgcccgtc accgtcagcc cttggcccgt
     1081 caaccggtaa atatacgttc cggtctcaac cagcttcccg tcgtcgtccc ggccgtccca
     1141 actgttgatg ccggagaggc gccgcacccg ctcgccccgc aaatcgaaaa attccacgtc
     1201 gaactcgccc gagatgttga actgggcgaa atcgttttgt ccgtcgccgt tgggcgtgat
     1261 gattttttcc tgggggcgga ccatgtcggg ggtcaacgtc gtcgccagag gggccaggct
     1321 gtagacgctg aagtgggaga ccgtcacgct cacccggttg gccaccgggt ccacctgccc
     1381 gcccacgaac cgccagaggt ggccgtcgta ccaaaacacc cgcaacagcg tttcgtcgac
     1441 gccgctcccg tccacccgtc cgtcctggtc ctggtcgggg tagggaatgc tgagggtgac
     1501 cggccggctg aagcgaaggc cttcgggcgt cagatcgaag acccgcaacg gaacgccgcc
     1561 ctccaccggt gggtaatggg tggagggctt gagcgtgacc tgaatggttt ggtcgccgtt
     1621 caggctgcgg gg
//
LOCUS       DAOQLS010000535         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57319_length_1629_cov_3.770648, whole genome shotgun sequence.
ACCESSION   DAOQLS010000535 DAOQLS010000000
VERSION     DAOQLS010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57319_length_1629_cov_3.770648"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PK362_09200"
     CDS             complement(<1..1123)
                     /locus_tag="PK362_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75042.1"
                     /translation="MIKRVTPIAAAFFCLLIVPDLHALEEVGVDDFRVSRMGVDGVPT
                     TGILPFSHIAQRIQAVAYNSQDNEYLVVYAGDTDDDPPADDEIEVFGQRIDGTSGDAI
                     GGQLRLTTAGTDFDLAVFARSPAIAYDRQRNEYLLAFESNAGNNREIWVQRLAADGTR
                     LGNETQISGFADLDGDTAGVGCYSPSVAYNPNTDDYLVVWQSGATDGLMEIYGRLVNG
                     ADGQPHAAENFRISDMGPDGNESYFARFPAVAVNTRANEYLVVWEGQDDQAPNDVSEN
                     EIFAQRLDENGAEIGVNDFPVSDMGALGSTDTNAHSPRVAYNPLDNQYLVVWEGNEEE
                     PVDVCNDGCMDIKYEIYGQRLTAAGAETGDDDFRIGTWDN"
     gene            complement(1189..>1629)
                     /locus_tag="PK362_09205"
     CDS             complement(1189..>1629)
                     /locus_tag="PK362_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNC75043.1"
                     /translation="VLKDMGRWYVFEKYRERVEFIRRHLPDCGLTADVLVGFPTETEA
                     AFENTYARIESLALSGLHVFPYSQRPGTKAAELPPLAHDVQRRRVEALLALGEKLKAQ
                     FRARFAGTVRQVVTEPEGEGWTENYIRVPHDRNLPPGRLAAVRI"
BASE COUNT          267 a          498 c          539 g          325 t
ORIGIN      
        1 ggttgtccca ggtgccgatc cggaaatcgt cgtcccccgt ttccgcgccc gccgcggtca
       61 accgctggcc gtaaatttcg tatttgatgt ccatgcaccc gtcgttgcag acgtccacgg
      121 gctcttcctc gttgccttcc caaaccacca ggtattggtt gtccaggggg ttgtaggcca
      181 cccggggcga atgggcgttg gtgtccgtgg aacccaaggc ccccatgtcg ctcacgggaa
      241 aatcgttcac gccgatctcg gcgccgtttt cgtccaggcg ctgggcgaaa atttcgtttt
      301 ccgagacgtc gttcggggct tggtcgtcct ggccttccca aacaaccagg tattcgttgg
      361 cgcgggtgtt caccgccacg gcggggaacc gggcgaaata ggattcgttg ccgtcgggcc
      421 ccatgtcgct gattcggaaa ttttcggccg cgtggggctg gccgtccgcg ccgttcacga
      481 ggcggccgta gatttccatc aggccgtccg tggcgccgct ttgccagacc accagataat
      541 cgtccgtgtt cgggttgtaa gcgacggacg gcgagtagca tccgaccccc gccgtgtccc
      601 cgtccaggtc ggcaaaaccg ctgatctggg tttcgttgcc caaccgggtg ccgtccgccg
      661 ccaaccgttg gacccaaatt tcccgattgt tgccggcgtt gctttcaaag gcgaggaggt
      721 attcgttccg ctggcggtcg taggcgatgg ccggggaacg cgcgaagacc gcgagatcga
      781 agtccgtccc cgccgtggtc agccggagct ggccgccgat ggcgtcgccg gaggtcccgt
      841 cgatccgctg gccgaaaacc tcgatttcgt cgtcggcggg cgggtcgtcg tcggtgtcgc
      901 cggcgtagac caccaggtat tcgttgtcct ggctgttgta ggccacggcc tggatccgct
      961 gggcgatgtg gctgaaaggg agaatgccgg tcgtgggcac gccgtccacc cccatgcgac
     1021 tgacccggaa atcgtcgacc cccacttctt ccagggcgtg gagatcgggg acgatgagga
     1081 ggcaaaaaaa cgccgccgcg atgggggtga cgcgcttgat cattttatct cgttgtcgga
     1141 agttgaaatg aaggagcgga ggagccgcgg tattatacca cggaaattct aaattcggac
     1201 ggccgccagg cgccccgggg ggaggtttcg gtcgtgggga acgcgaatat agttttcggt
     1261 ccagccttcc ccctcgggtt cggtgaccac ctggcgcacg gtgccggcga agcgggcccg
     1321 gaattgggct ttcaattttt cacccagggc gaggagcgcc tccacccgac ggcgctgcac
     1381 gtcgtgggcc aggggcggga gctcggcggc cttggtgccc ggccgttggg aataggggaa
     1441 cacgtgcagg cccgagaggg ccagggactc gatgcgcgcg taggtgtttt caaaagccgc
     1501 ctcggtttcc gtcgggaaac ccaccaggac gtccgcggtc aaaccgcagt ccggcaggtg
     1561 gcggcgaatg aattccaccc gctcccggta tttttcaaag acgtaccacc ggcccatgtc
     1621 ctttaagac
//
LOCUS       DAOQLS010000536         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57321_length_1629_cov_3.727446, whole genome shotgun sequence.
ACCESSION   DAOQLS010000536 DAOQLS010000000
VERSION     DAOQLS010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57321_length_1629_cov_3.727446"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1629
                     /locus_tag="PK362_09210"
     CDS             <1..>1629
                     /locus_tag="PK362_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75044.1"
                     /translation="DRGWSDPEHLWHDGWVDANKNGKVDTGEIDNDAADGVVQDAEIE
                     WVDANGNGIADAGRVVDTGSSTGSIVQDYAVFDRDVRLIRSTTTTRQTTTGDNETEQS
                     IVTEYTYDSAGRMTGASSQGETYTLVRGWSDTTVPPDGVNDEVLKVGETIGTFQQTFL
                     IIKGNAKVWETVSTNETMNINGSQNIGDMTTTYSYNQNGLVTGAVGRGHSQNIDVGWT
                     DTDVPPDGEVDELIVTGQNQSTITQEFRVINGQAKVYRTVTDTLSVDPNTGQPIENDK
                     DHYNHSISTVVNVYDAVGRLVAADGNTVFDSTALVASQPGSDTLLENAINPPPAAPVT
                     ASAGTAGTATVAPHGSAAAGQRARTPIENAVGGDLPTPSDEAGAQKIANQELPNGGTR
                     QIFARREEDGTITYRLMDTFLDGEGGRQETINLWRSDADGNTLGTPLRLFPAVTRPAQ
                     TEGESNQIGLSNLLDSIGWESGNPFAYRTLQAGTPNITPGWRYNPLTRSLERLGQALP
                     ENNRLGLEGNRHAVSQSWRDVAVTGLVLSGEESS"
BASE COUNT          372 a          540 c          476 g          241 t
ORIGIN      
        1 aggaccgcgg ctggtccgac ccggaacatc tctggcacga cggctgggtg gacgccaaca
       61 agaacggcaa agtcgacacg ggtgaaatcg acaacgacgc cgccgacggc gtcgttcaag
      121 acgcggaaat cgagtgggtg gacgcgaacg gcaacggcat cgccgacgcg ggccgggtcg
      181 tcgacaccgg ttcgtccacg ggttcgatcg ttcaggatta cgccgttttc gaccgggacg
      241 tgcgcctgat ccgctccacg accaccacgc gacaaaccac cacgggcgac aacgaaaccg
      301 agcagtcgat tgtcaccgaa tacacctacg attccgccgg acgaatgacc ggcgcgtcct
      361 cccaaggcga aacctacacc ttggttcggg gatggtcgga caccaccgtt ccgccggacg
      421 gcgtcaacga cgaagtcttg aaggtcggcg aaaccattgg caccttccag caaacgttct
      481 taatcatcaa aggcaacgcc aaagtgtggg aaaccgtcag cacgaacgaa acgatgaaca
      541 tcaacggcag ccaaaacatc ggcgacatga cgacgactta ttcctacaac caaaacggcc
      601 tcgtcaccgg cgcggtgggc cgtgggcatt cccagaacat cgacgtgggg tggacggaca
      661 ccgatgttcc gcccgacggg gaagtggatg aactgattgt cacgggccag aaccagtcga
      721 ccatcacgca ggaatttcgg gtgatcaacg gccaggccaa ggtctatcgg accgtgaccg
      781 acacgctttc ggtcgacccg aacaccggcc aacccatcga aaacgacaag gaccactaca
      841 accactcgat ttccaccgtg gtcaacgtct acgacgccgt gggccggttg gtggcggcgg
      901 acgggaacac cgttttcgac agcaccgccc tggtggccag ccagcccggt tccgacaccc
      961 ttttggaaaa cgccatcaat ccgcccccgg cggctcccgt cacggcctcg gccggcacgg
     1021 cgggcaccgc gaccgtcgcc ccccatggtt cggccgcggc cggtcaacgc gcccgcacgc
     1081 ccattgaaaa cgccgtgggc ggcgacctcc cgaccccctc ggatgaagcg ggggcccaaa
     1141 aaatcgccaa tcaagaattg cccaacggcg ggacccggca aattttcgcc cgacgcgaag
     1201 aagacggcac gatcacctat cgactgatgg acaccttttt agacggggaa ggcggacgcc
     1261 aagaaaccat caatctctgg cggtccgacg ccgacggcaa cacgctgggg acccctctcc
     1321 gacttttccc cgccgtaacg cgtccggctc aaacggaagg cgaatcgaac caaatcggtt
     1381 tgtccaacct cctggattcc atcggttggg aatcgggcaa tccgtttgcc tatcgaacgc
     1441 ttcaagccgg aacgccgaac atcaccccgg gttggcgtta caaccccctg acgcgaagcc
     1501 tcgaacgcct gggtcaggcg ctgccggaaa acaatcggtt ggggctcgag ggcaaccggc
     1561 acgcggtttc ccaatcgtgg cgggacgtcg ccgtgaccgg actcgtattg tccggggagg
     1621 aatccagcc
//
LOCUS       DAOQLS010000537         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57382_length_1628_cov_3.921170, whole genome shotgun sequence.
ACCESSION   DAOQLS010000537 DAOQLS010000000
VERSION     DAOQLS010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57382_length_1628_cov_3.921170"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..110
                     /locus_tag="PK362_09215"
     CDS             15..110
                     /locus_tag="PK362_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75045.1"
                     /translation="MDRLMEFCWKFLLPWSLVNVALAGAVFLYRS"
     gene            107..637
                     /locus_tag="PK362_09220"
     CDS             107..637
                     /locus_tag="PK362_09220"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNC75046.1"
                     /translation="MNYLKTVLTNSWALLKGLKITLWHMFSPAITVQYPYEKLALSDR
                     YRGALAFHPDVCISCEMCVRACPSNCISLEAKRNEETKKKDLAWYKIDFGKCNYCRLC
                     EEICPTKPKSVHHTQEYELSFSNRGEFVQEWKTSVPQPAASEAGQIWGAYVARGQKLG
                     PNPATNASATGSSPVA"
     gene            639..1127
                     /locus_tag="PK362_09225"
     CDS             639..1127
                     /locus_tag="PK362_09225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNC75047.1"
                     /translation="MIAKLVFAFLAVLVLVPAGLAVTVKNIFHSALWLVLSLTGVAGL
                     FALLGADFLFVAQILVYTGGITVLLLFVVLLSGSPKDWIVRQVNGQWAWGLALSAIFV
                     GLLATLFKQIPEPAELTPAAPTSAPLGLMLVRDMLLPFEAVSLVLLAALVGAIHFSKR
                     NS"
     gene            1127..1435
                     /gene="nuoK"
                     /locus_tag="PK362_09230"
     CDS             1127..1435
                     /gene="nuoK"
                     /locus_tag="PK362_09230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNC75048.1"
                     /translation="MLPLFGYTLVSGVLVSLGLFGALARRNTLSILIAIELIFNAANI
                     NLVAFNRYLHPGQPAGQAFAIFVIALAAAEAVVGLALVLTVYRQFKTVLSEDLNLLKG
                     "
     gene            1437..>1628
                     /locus_tag="PK362_09235"
     CDS             1437..>1628
                     /locus_tag="PK362_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNC75049.1"
                     /translation="MLDHAYLIPLLPLAASVLILFFGRWLPLEGAFIGIAVAAWGLVH
                     AGFILGAVYLHPVDVLVHEG"
BASE COUNT          275 a          511 c          440 g          402 t
ORIGIN      
        1 acccccgttt gcgggtggac cgactcatgg aattttgctg gaaatttctc ctgccctggt
       61 ccctggtgaa cgtggccctg gccggcgccg tcttccttta ccgatcatga actaccttaa
      121 gaccgtcctt accaacagct gggcgctatt gaaagggttg aaaatcaccc tttggcatat
      181 gttttcgccg gccatcacgg tgcaataccc ctacgaaaaa ttggcccttt ccgaccgcta
      241 ccgcggcgcc ctggcctttc accccgatgt ctgtatttcc tgcgaaatgt gcgttcgcgc
      301 ctgcccctcg aactgcatct ccctggaagc caaacggaac gaagaaacca aaaagaagga
      361 tttggcttgg tacaaaatcg acttcggaaa atgcaattac tgccgccttt gcgaggaaat
      421 ctgcccgacc aagccgaagt cggttcacca cacccaggaa tacgaattgt ccttctccaa
      481 ccggggcgaa tttgttcaag aatggaaaac gtcggtgccc caacccgccg cttcggaagc
      541 gggacaaatt tggggcgcct acgtggcgcg cggacaaaaa ctcggaccca accccgccac
      601 aaacgcttcg gcgacggggt cctcgccggt ggcctgacat gatcgctaaa ctggttttcg
      661 cgttcctggc ggttttggtg ctggttccgg cgggactcgc cgtgaccgtg aaaaacatat
      721 ttcacagcgc cctgtggctg gtgttgagtc tcaccggcgt cgcggggttg tttgccttgc
      781 tgggcgcgga cttccttttc gtcgcccaga tcctggtcta caccgggggc atcaccgtcc
      841 ttctactttt tgtggttttg ttgtccggca gccccaagga ttggatcgtc cggcaggtca
      901 acggtcaatg ggcctggggc ctggccctct cggccatttt tgtcggtttg ttggcgaccc
      961 tcttcaaaca gatacccgaa cccgcggagc tcacccccgc ggccccgacg tcggccccgt
     1021 tgggcttgat gctggtgcgg gacatgctcc ttccttttga agccgtttcc ctggtcctcc
     1081 tcgccgcatt ggtgggggcc atccactttt ccaagaggaa ttcctaatgc ttccgctttt
     1141 cggttatacc ctggtgagcg gggtgctggt gtccctgggc cttttcggcg ccctggcgcg
     1201 gcgcaacacc ttgagcatcc tcattgccat cgaattgatt ttcaacgcgg ccaacatcaa
     1261 tctggtggcc ttcaaccgct atttgcaccc cggtcaaccc gcgggccagg cctttgccat
     1321 attcgtcatc gccctggccg cggccgaagc cgtggtgggt ttggccctgg ttttgacggt
     1381 ctaccgtcaa ttcaaaacgg tcctgtccga agacttgaat ctcctgaagg gttaacatgc
     1441 tcgaccacgc ctacctcatc ccgcttttgc ccctggccgc ctcggtcttg atccttttct
     1501 tcggccgttg gcttcccctg gaaggggctt tcatcggcat cgccgtcgcc gcctggggtc
     1561 tggttcacgc cggctttatt ttgggcgcgg tttaccttca tcccgtcgac gtgttggtgc
     1621 acgaaggc
//
LOCUS       DAOQLS010000538         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57488_length_1626_cov_6.568428, whole genome shotgun sequence.
ACCESSION   DAOQLS010000538 DAOQLS010000000
VERSION     DAOQLS010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57488_length_1626_cov_6.568428"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1626
                     /locus_tag="PK362_09240"
     CDS             21..>1626
                     /locus_tag="PK362_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75050.1"
                     /translation="MTAATANAINAAVKTTTPEQRETLSVALELKGDKAVQRVPLETG
                     DGWATTEHAIDWPTIGNLKEAVFAVTPAPGRSSRGDLYFDLSFLQGGTPDLAGAGAPG
                     AFGLLNAGKRGVFNMGASDGRILVAFDDTVKKDLIRFEFTAASGSAVGVWSSEYPPAL
                     TPETANGVRVAVHAADAAQAAAVAVAVEIKGLKETQTVEVPLHEGWTTVREGVDWKTI
                     GGLKEAVFVVKPTPGGGSVNGALTLDLEFIQGDFVLKKGPRVGGKIGLVLLTGFLLFL
                     ATALVGRWIRGRSPSVPAPDAIPVPGMGRDLLMGSLLTAGLVGTVATFVVGSLPGSEG
                     SFGFLLLGALGALVGEGATRILAQRPGTPGEVFLNFVYTGLVAASSSLQPLWLAPTNG
                     AHIFLNNHLTATVLFALYHGVNAASLASTGKPLRPVVGALLTGVPTLFGALLALQSAG
                     FVEGLADALTGGGFAGRGLLLQTLGRVLLVFAVNEAVANAIGLAVKGKGLTSARGHLL
                     LFAVSLGVVVAQRSKCPRAEVRPLGIC"
BASE COUNT          242 a          529 c          548 g          307 t
ORIGIN      
        1 ccaagggata cccggccgac ttgacggccg ccaccgccaa cgccatcaac gcggcggtta
       61 aaacgaccac cccggagcaa cgggaaacct tgtccgtcgc ccttgagttg aagggcgaca
      121 aggccgttca gcgcgttccg ttggaaacgg gcgacgggtg ggccaccacc gaacacgcca
      181 tcgactggcc gaccatcgga aatctgaagg aagccgtttt tgccgtcacc cccgcccccg
      241 gccgatccag ccggggcgac ctgtatttcg acctttcttt cctccaggga ggaacgccgg
      301 atctcgccgg ggccggcgcc ccgggcgcct tcgggttgtt gaacgccggc aagcgcgggg
      361 tgttcaacat gggggcctcg gacggccgga tcctcgtggc ttttgacgac accgtcaaaa
      421 aggacctgat tcgatttgaa ttcaccgccg ccagcgggtc cgccgtgggg gtgtggagca
      481 gcgagtatcc cccggccttg acgccggaaa cggccaacgg cgttcgcgtg gcggtccacg
      541 ccgcggacgc ggcccaggcg gccgccgtgg cggtggccgt tgagatcaag ggcctcaagg
      601 aaacccagac cgtggaagtt cctctccacg aagggtggac gacggtgcgg gaaggggtgg
      661 attggaaaac catcgggggg ctcaaagaag cggtgttcgt cgtcaagccg accccgggcg
      721 gcggatccgt caacggggcg ctgacgctgg atctggaatt catccaaggc gattttgtcc
      781 tgaagaaagg cccccgggtc ggcggtaaga tcggcttggt gttgctgacg ggcttccttc
      841 tttttctcgc gacggctttg gtcggccgat ggatccgcgg tcggtcgccg tcggttccgg
      901 ccccggacgc catccccgtt ccggggatgg gtcgggatct cttgatggga tcccttttga
      961 cggccggcct cgtcgggacg gtggccacct tcgtggtggg ttccctcccg gggtccgaag
     1021 ggtctttcgg gtttctgttg ttgggcgccc tgggcgcctt ggtgggcgaa ggcgccaccc
     1081 ggattctggc tcaacgcccg ggcaccccgg gcgaagtctt cctgaatttt gtctacaccg
     1141 gcttggtggc cgcgagctca agccttcagc ccctgtggtt ggcgcccacc aacggcgccc
     1201 acatattcct gaacaaccac ctgacggcga cggtgttgtt cgcgctctac cacggggtca
     1261 acgccgcttc cctcgcctcg accgggaaac cccttcggcc ggtggtcggc gcgctcctca
     1321 ccggcgttcc gacccttttc ggcgcgctcc tggcccttca gagcgccgga ttcgtcgagg
     1381 gcctggccga cgcgctcacc gggggcggtt tcgccggacg cggcctgctt ctccagacct
     1441 tggggcgggt tttgctggtg ttcgcggtca acgaagccgt ggccaacgcc atcgggctcg
     1501 ccgtcaaagg caagggcctc acctcggccc gtgggcattt gcttcttttc gccgtgtcgc
     1561 tcggcgttgt cgtggcgcaa agaagcaaat gcccacgggc cgaggtgagg cccttgggca
     1621 tttgct
//
LOCUS       DAOQLS010000539         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57505_length_1626_cov_3.887333, whole genome shotgun sequence.
ACCESSION   DAOQLS010000539 DAOQLS010000000
VERSION     DAOQLS010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57505_length_1626_cov_3.887333"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK362_09245"
     CDS             <1..598
                     /locus_tag="PK362_09245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC75051.1"
                     /translation="APRPPDETVRRSLGLPPGVPVVGNLTHYSWWKGQSFFLEAARKV
                     LDAGIRAHFLLVGKDTDGGDALNRVHALGIDDQVTLAGFRTDMPEILSTLSASVVSSL
                     AGEGFSGVLRESMCLGIPVVATAVGGNGELVKNEKTGLIVPPADADALAAAIGRLLTE
                     PELAQRMAREARTNVVENYSIDNMVAKTIKLYERLVFA"
     gene            595..1401
                     /locus_tag="PK362_09250"
     CDS             595..1401
                     /locus_tag="PK362_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75052.1"
                     /translation="MKPLWAAVLLLLAGAARAGEWSVQYLPQVRTEYEGTGFRQVPNG
                     WELKVKPWTPAGGLRWRQSLAPKLSLQAQYWVNRSAYFKEDGNTQVGPLRQTGLTRQS
                     LQALWVDLRRPLSGSSVEAVGGLHGIYQSFRRKDIVFNGTGDPSSTLDSQTGLGAQLG
                     FHFGRLLPPMERRGFFWDGELLLGRLLWTRNNRRTEGGGIHSGGYTYSFRLEAGRAMG
                     PLRLSLGYTRQMYEILVPGGRALPSGAAGSLPINKTDFFGPFLSLGWAYQ"
     gene            1398..>1626
                     /locus_tag="PK362_09255"
     CDS             1398..>1626
                     /locus_tag="PK362_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75053.1"
                     /translation="MNLRSLALGCLAAASLSVARSAETGGTWSLTLVPGATLRYRGEG
                     KILPQGYLNRTENQNAPLYRLEWRQPADPSGD"
BASE COUNT          273 a          521 c          516 g          316 t
ORIGIN      
        1 cgcgccccgg ccgccggacg aaacggttcg ccgttccctg ggtcttcccc cgggggtgcc
       61 ggtggtggga aatttgaccc actattcctg gtggaaggga caatcttttt ttctggaagc
      121 cgcccggaaa gtgttggacg cgggaattcg ggcccatttc ctcctggtgg gcaaagacac
      181 cgacggcggc gacgccttga accgtgtcca cgccctgggc atcgacgacc aggtgaccct
      241 ggcgggattc cgcacggaca tgcccgaaat cctttccacg ttgtcggcga gcgtggtgtc
      301 gtccctggcg ggggaggggt tctccggcgt gcttcgggaa tccatgtgcc tgggaatccc
      361 cgtcgtggcc acggccgtgg gggggaacgg ggaattggtg aaaaacgaaa agacgggact
      421 gatcgtcccg cccgcggacg ccgacgccct ggccgccgcc atcgggcgcc tgctcaccga
      481 accggaattg gctcaacgca tggcccggga ggcccggaca aacgtggtcg aaaattactc
      541 gatcgataac atggtcgcta aaacgatcaa actctacgaa cgcttggttt tcgcgtgaag
      601 cccctttggg ccgcggtgtt gttgcttctc gccggggccg cccgggcggg ggaatggtcc
      661 gttcaatacc ttccccaagt tcggaccgaa tacgaaggca cggggtttcg ccaggtgccc
      721 aatgggtggg aacttaaagt caaaccgtgg accccggccg gcggtctgcg ctggcggcaa
      781 tccctggccc cgaaactctc tctccaagcc caatactggg tgaaccgctc ggcctatttt
      841 aaagaagacg gcaacaccca ggtcggccct ctccgtcaaa ccggattgac ccgccaatcc
      901 cttcaagccc tgtgggtgga ccttcggcgc cccctgtcgg gctcctccgt ggaagcggtg
      961 ggcggccttc acgggatcta ccaaagcttt cgacggaaag acattgtgtt caacggcacg
     1021 ggcgacccgt cgtccacgct ggacagtcaa acgggtctcg gcgcgcaatt gggttttcat
     1081 ttcggccgcc ttttgccgcc gatggagcgt cgcggatttt tttgggacgg cgagctcctc
     1141 ctgggccgat tgctttggac gcggaacaac cggcgcaccg aaggcggcgg cattcattcg
     1201 ggcggctaca cctactcttt tcgcctcgag gccggccgcg cgatggggcc gctccgcctc
     1261 agcctgggct acacccggca gatgtatgaa attctggttc ccggcggccg ggccctgccg
     1321 tcgggcgcgg cgggatccct tcccatcaac aaaaccgatt tcttcggacc gttcctgtcc
     1381 ttggggtggg cgtaccaatg aaccttcgct ccctggccct cgggtgcctg gccgcggctt
     1441 ccctctccgt cgcccgttcg gcggaaaccg gtggcacctg gtcgttgact ctcgtgcccg
     1501 gcgcgacgct ccgttaccgg ggcgaaggga aaattttgcc ccaggggtat ctcaatcgga
     1561 cggaaaacca aaacgccccg ctgtatcgtt tggagtggcg gcaacccgcc gatccctcgg
     1621 gggatt
//
LOCUS       DAOQLS010000540         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57531_length_1626_cov_2.732018, whole genome shotgun sequence.
ACCESSION   DAOQLS010000540 DAOQLS010000000
VERSION     DAOQLS010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57531_length_1626_cov_2.732018"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PK362_09260"
     CDS             complement(<1..1058)
                     /locus_tag="PK362_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNC75054.1"
                     /translation="MSDQLTKGQFPLGPVRQAVRDDGLDGWMFFYFQNNDPLALRLLR
                     VPANKFFTRRWFYFVPGRGAPVKLVHRIEPDALDHLPGRTLFYASWRELETGLARTLR
                     GARQVAMQYSPKNAVPYISRVDAGTVELVRSLGPRVVSSGDLVQRFESVLTPGQWRQH
                     QQTARHLHQIVRKAFAFIGRSVREKGKITEFDVQRFILREYARRHLITNAEPIVAVNK
                     NSARPHYAPTRARHAPVQKGDWVLLDIWAKPRGAEAVYADITWCGYVGAKTPEKHERI
                     FQIVRRARDAAVEFVQNRLAEGKAVRGWEVDRAARSVIQRAGYGKYFIHRTGHSIGTE
                     DHANGANMDGLETRETRRL"
     gene            complement(1051..>1626)
                     /locus_tag="PK362_09265"
     CDS             complement(1051..>1626)
                     /locus_tag="PK362_09265"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC75055.1"
                     /translation="PVLKKIWRLARALVPPDGARHFNSALMDLGATVCKPANPDCGAC
                     PLAAGCWANRNGRVSDLPVVGAEGPKKLLHFHAAIVEQGGRWLLRRRPPTGLYAGLWE
                     FPGLEFKDSPPAAQVARFLRERFQAAPSSPRPLPALRHTLSHREIVVHPWRCKLEKTA
                     GGHRWFPAKDIDRLAISSLTRRVWQQVRFHV"
BASE COUNT          288 a          495 c          554 g          289 t
ORIGIN      
        1 aggcgccggg tttcccgggt ctccagcccg tccatgttgg cgccgttggc gtggtcctcg
       61 gttccgatgg aatggcccgt gcggtggatg aaatactttc cgtaaccggc gcgctggatg
      121 accgaccggg ccgcccggtc cacttcccac cctcgcaccg cctttccctc cgccaggcga
      181 ttttgcacga attccaccgc ggcgtcccgg gcccggcgga caatttgaaa aatccgctcg
      241 tgtttctccg gcgtttttgc cccgacgtag ccgcaccagg tgatgtcggc gtagaccgcc
      301 tccgctcccc ggggcttggc ccagatgtcg aggaggaccc agtctccttt ttggacgggg
      361 gcgtgtcgcg cccgggtcgg cgcgtagtgg ggccgagcgc tgtttttgtt cacggccacg
      421 atgggctcgg cgttggtgat gagatgccgg cgggcgtatt cccggaggat gaaacgctgg
      481 acgtcgaatt ccgtgatctt tcctttttcc cggaccgagc ggccgatgaa ggcgaaggcc
      541 ttccggacga tttggtggag gtgccgcgcg gtttgttgat gttgccgcca ttggcccggc
      601 gtcagcaccg actcgaaccg ttgaaccaaa tcccccgaag aaaccacccg ggggcccagg
      661 gaccgcacga gctccacagt gccggcgtcc acccgggaaa tgtagggcac cgcgtttttg
      721 ggcgaatatt gcatcgccac ctggcgggcc ccccgcagag tacgggccag gccggtttcc
      781 agctcgcgcc aggaggcgta gaaaagtgtt cggccgggca agtgatcaag ggcgtcgggt
      841 tcgatgcgat gcactaattt aacgggcgcc ccacggcccg gcacgaaata gaaccagcga
      901 cgggtgaaaa acttattggc ggggacccgg agaaggcgaa gagccagggg atcgttgttt
      961 tggaaataga agaacatcca accgtccaat ccatcgtcgc ggaccgcttg ccggacgggg
     1021 cccaacggaa attggccctt ggtcaattga tcagacatgg aaccggacct gttgccacac
     1081 gcggcgggtc aaacttgaaa tggcgaggcg gtcgatgtct ttggcgggga accatcgatg
     1141 acccccggcg gtcttttcga gcttgcatcg ccagggatgg acgacgattt cccggtggga
     1201 cagggtgtgt cgaagggccg gcaggggccg gggcgacgaa ggagcggcct ggaatctttc
     1261 ccgaaggaac cgggccacct gggccgccgg cggcgaatct ttgaattcaa gtccgggaaa
     1321 ttcccaaagc ccggcgtaaa ggcccgtggg cggtcggcgg cggaggagcc agcggccgcc
     1381 ctgttcaacg atggccgcgt ggaagtgcag aagttttttc ggaccttcgg cccccaccac
     1441 ggggaggtcc gagacgcgac cgttccggtt cgcccaacac cccgcggcca gggggcacgc
     1501 cccgcaatcg gggttggccg gcttgcagac cgtggccccc aggtccatga gggcggaatt
     1561 gaagtgccgg gcgccgtcgg gtggaacgag cgcccgggcc aaacgccaaa ttttcttaag
     1621 gaccgg
//
LOCUS       DAOQLS010000541         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57649_length_1624_cov_3.365838, whole genome shotgun sequence.
ACCESSION   DAOQLS010000541 DAOQLS010000000
VERSION     DAOQLS010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57649_length_1624_cov_3.365838"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PK362_09270"
     CDS             <1..413
                     /locus_tag="PK362_09270"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HNC75056.1"
                     /translation="TVTTLTLAVSLTGGFRANPERTERWGTTVLYVLLASLGARASLA
                     ALGRAPYFLAVGACILAVHAVILWAGGRLFRLPLFLLASASQACVGGVVSAPMVSAVY
                     RPALSAVGLLLAVTGNVVGTYFGLVVAQISSRWG"
     gene            424..>1624
                     /locus_tag="PK362_09275"
     CDS             424..>1624
                     /locus_tag="PK362_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75057.1"
                     /translation="MKRWAIVLLVAGPSVLRAEPPASVEMGDDFPPVQISSGPALAST
                     APTPAPGAPLRVVHPPEGASLPYVKSSFVLGSVDPGSRLSIDGQSIRVYPGGGWIAMV
                     SYTPGANDIEFEVTRGTDVFNFTRRVYVAGPPGVPPVKPLNVRVLTPDVDAEVTAGDA
                     ITVTAVGSPGQEAGFVFSGSKKKHPLAESAVSRGTYRGSYVVRPGDEFEGASIKVTLT
                     AKNGAHDTSTAPGKIWRFDEESPWVVEVSTDLAILRAASAPFPGEKAGYVMFPPPGTR
                     LWVTGRRGNELRVRLSAAREAWIGADEVRFLPSGTPPPRTLAGAVSVEPAGRHTVVRM
                     NLGQRVPMEVRPLDDGAGLDVLFYGVASNTDWVHYNAPQGAVRRVEWFQDDADTYRLR
                     IHTRSRRW"
BASE COUNT          211 a          547 c          549 g          317 t
ORIGIN      
        1 tgacggtcac gaccctcaca ctggccgttt ccctgacggg agggttccgc gccaaccccg
       61 agcgtaccga acgctggggg accacggtgc tttatgtcct gttggcctcc ctcggcgccc
      121 gggcctcctt ggccgccctg ggccgggccc cctattttct cgcggtcggc gcttgcattt
      181 tggccgttca tgcggtaatt ctatgggcgg gcggtcgcct gtttcggctt ccgctttttt
      241 tattggcctc ggccagtcag gcgtgcgtcg ggggggtcgt gtcggcgccg atggtgagcg
      301 ctgtttaccg gcccgccctt tccgccgtgg gtctgctgtt ggcggtgacg ggaaacgtgg
      361 tcggcaccta ttttgggttg gtcgtggctc agatttcctc ccggtgggga tgaaggaact
      421 tccatgaaac gttgggcgat cgttttgttg gtggcgggac cgtcggtgct tcgggccgag
      481 ccgccggcgt ccgttgaaat gggcgacgat tttccgccgg ttcaaatcag ttcgggcccc
      541 gccctcgcct ccaccgcccc gacgcccgcg cccggcgccc ccttgcgggt cgtccatccg
      601 cccgaggggg cctccctgcc ctacgtgaaa tcctcttttg tcctgggttc cgtggacccg
      661 ggttcccgcc tctcgatcga cggccagtcg atccgggtct atcccggcgg gggctggatc
      721 gccatggtga gctacacccc gggggccaac gacatcgaat ttgaagtgac ccgcggcacc
      781 gatgttttca acttcacgcg gcgcgtctac gtggccggtc cgccgggcgt tccgccggtc
      841 aagccgctca acgtgcgcgt gttgacgccg gacgtcgacg ccgaagtcac cgcgggggac
      901 gcgatcacgg tgacggccgt cggcagtccg gggcaggagg ccgggttcgt tttttcgggg
      961 agcaaaaaaa agcaccccct cgccgaaagc gccgtgtccc ggggaaccta ccggggttcc
     1021 tacgtggtgc ggcccggcga cgaattcgag ggcgcttcga tcaaggtcac gttgacggcc
     1081 aaaaacggcg cccacgacac ctccacggcc cccggaaaaa tttggcggtt cgacgaggag
     1141 tctccctggg tggtcgaagt ctcgacggat ttggccatcc tgcgggcggc ttccgccccc
     1201 tttcccggcg aaaaggccgg gtacgtcatg tttccgcctc cgggaacccg gctgtgggtg
     1261 accggccgcc gcgggaacga gctgcgggtc cgtctctccg ccgcccggga ggcctggatc
     1321 ggcgccgacg aagtccgctt tttgccgtcg ggcacgccgc cgccccgcac cctggccggg
     1381 gccgtctccg tggaacccgc cggccgccac accgtggtcc ggatgaattt gggccagcgg
     1441 gtgccgatgg aagttcgccc cctggacgac ggcgcggggc tggatgtcct tttttacggg
     1501 gtggcctcca acaccgattg ggtgcactac aacgcgcccc agggcgcggt tcgccgggtg
     1561 gaatggttcc aggacgacgc ggacacctac cggcttcgaa tccacacccg ctcccggcgc
     1621 tggt
//
LOCUS       DAOQLS010000542         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57716_length_1623_cov_4.133929, whole genome shotgun sequence.
ACCESSION   DAOQLS010000542 DAOQLS010000000
VERSION     DAOQLS010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57716_length_1623_cov_4.133929"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PK362_09280"
     CDS             <1..961
                     /locus_tag="PK362_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNC75058.1"
                     /translation="PSSAPQARGNAVLSGVGLTPMPGASLEGLGGSIAFSEDDAALKD
                     FKGKFLGSPFTLALTARRFARPELTYDFKLDRLDLAKLQEIFGSTAAAESPATPAGPA
                     GPAPIARAEGSIKIGEATHPYYLGKNFQLKGLLTDLGPDLGQLSGALTLSASDGKIRN
                     LPLAEKINKLMKKDAADLTYKTLGGHFRIERGLLTTEDLAVESDQTDLAAKGRVRLTD
                     FDSDLRATVKLPAGSLGGSLGDWSSGPDGRPTLTAKITGPLTDPKISVDLSQAAKNAA
                     KDLLQKSLEKYRKPAEGDNGGSTAVDKAVDQGLKSLQKLFKKK"
     gene            1009..1584
                     /locus_tag="PK362_09285"
     CDS             1009..1584
                     /locus_tag="PK362_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75059.1"
                     /translation="MDNASAPIASRLEIQDTPLGLEIVVTPQRVWPVLLFLSFWLCGW
                     AAGETAALKSLFVSKTPLPARLFLLVWLTGWTAGGGFALALCLWMMGGRERITVNALR
                     LSVAYEVFGKGWGREYELAKIKNLRLASDDPAAPAAAGARAKLGKTGRLAFDVEGKTI
                     RFCSAANSEEATRALEALRRRYPSLGAARKF"
BASE COUNT          305 a          541 c          495 g          282 t
ORIGIN      
        1 cccctcgtcg gccccccagg cccgggggaa cgccgtcctc tccggcgtgg ggctgacgcc
       61 gatgcccggc gcttccctgg aaggcctcgg cggttcgatc gccttttcgg aagacgacgc
      121 ggccctcaag gattttaaag gcaaattcct gggatcgcct ttcactctgg ccctcaccgc
      181 ccgccgcttc gcccggccgg aattaacgta cgacttcaag ctggaccgcc tggacttggc
      241 caagcttcag gaaattttcg ggtcgacggc cgcggccgaa tcccccgcga cgcccgccgg
      301 tcccgcgggg cccgcgccca tcgcccgggc cgaaggctcc atcaaaatcg gggaggccac
      361 ccacccgtac tacctcggaa agaactttca gctcaagggc ctgttgaccg acctggggcc
      421 cgacctggga caactgagcg gggccctgac gctctcggcc tccgatggaa aaattcggaa
      481 cctcccgctc gcggaaaaaa tcaacaaact catgaaaaag gacgccgccg acctgaccta
      541 caaaaccttg ggcggccatt tcagaatcga acggggcctg ttgaccaccg aggacctcgc
      601 cgtggaaagc gaccaaacgg acctcgcggc caagggccgg gtccgtttga cggatttcga
      661 ttccgacctg cgggcgacgg tcaaacttcc ggccggttcc ctgggcggca gtttgggaga
      721 ctggtcctcc ggtcccgacg gccggcccac gctcaccgcc aaaatcacgg ggcccctgac
      781 ggaccccaaa atttccgtgg acctctccca ggccgccaag aacgccgcca aggacctgct
      841 ccagaaaagt ctcgagaaat accgcaaacc cgccgagggc gacaacgggg gttccacggc
      901 cgtggacaaa gcggtggatc aggggctgaa gtcccttcaa aaactcttca aaaaaaagtg
      961 accggagggg ttggccccgc cccgtttccg cgttatcctt aattcaccat ggacaacgcc
     1021 tccgcgccga tcgcttcgcg actggaaatc caggacacgc ccctgggact tgaaatcgtg
     1081 gtcacccccc aacgggtctg gcccgtcctt ctttttttat ccttctggct ttgcggttgg
     1141 gccgcgggcg aaacggcggc tctgaaatcc ctcttcgttt ccaaaacccc ccttccggcc
     1201 cgcctttttc tcctcgtgtg gttgaccgga tggaccgccg ggggcggatt cgccctggcc
     1261 ctttgtcttt ggatgatggg cgggcgggaa cggatcaccg tcaacgccct gcgcctgagc
     1321 gtggcctacg aggttttcgg caagggatgg gggcgggaat acgagctggc gaaaattaaa
     1381 aatttgcggt tggcgtccga cgatccggcc gccccggcgg ccgccggggc ccgggcgaaa
     1441 ttgggaaaaa cgggtcgttt ggcctttgac gtcgagggga aaaccatccg tttctgctcg
     1501 gcggccaact ccgaggaagc gacccgggcc ctggaagccc tacggcggcg ctacccgtct
     1561 ttgggggcgg cccgaaaatt ctagcgtctc aacgggcgcc gggaagggtt tcgagggagg
     1621 acg
//
LOCUS       DAOQLS010000543         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57821_length_1622_cov_2.723676, whole genome shotgun sequence.
ACCESSION   DAOQLS010000543 DAOQLS010000000
VERSION     DAOQLS010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57821_length_1622_cov_2.723676"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          286 a          479 c          575 g          282 t
ORIGIN      
        1 ccggggggtt ggggggccac cagccaggca aggcccccct atcccgccca atgcggccag
       61 aagttggccg cggtattggg gggagggggg ccgctccagg cctggcagcc ccggcagcgg
      121 tgacgtgcgc gccgttgttt ggcgtgcacg gcggcgcaat ggagccgggg ctaacggaag
      181 ccatcaccaa gcacggtctc ctgttgctac cccctcaact gaacctttgc tggaagacag
      241 gcggggcggc ggcctggggt tggccgccgc tggcagtttg gtgagcgggc ccacgcgcga
      301 ggcgcgccgg cgaaatgggc cgggccttgg gaacaaggct gggcccgcga atgctcttga
      361 ccttcggccc cggggttgtt ttttgacccc ggggacgact aaataggccc gagcccgctt
      421 tgctaccttt gcgcgcgcct ggaccgggag ggcgagggca agaccttcag ggattggcct
      481 tccccagcca acggagcggg gtgcccagcg gcggcgggtg aaacggagcg gaggtgcggc
      541 ggcgttctat gccgacgcgg cggagcgact gggttttgac cgagccgggg gaggcgagga
      601 aatggttgga gggcgctagc ccggaaaccg gacgaatcag gcggagcggc aaccgtccaa
      661 tcgcccgggc gaccgagcga gccgggcggc ggcgggggtt ttcctgccga cgcgcggcga
      721 gcgaaccggg gtggaacgtt caaccgaagc gggaggcgtc ggggtttttc aggaaggacg
      781 aaccaaccgg agcggagact tctttctttt ccagccccgg gctttcgggt ttccggggtc
      841 gggttttaat ggggccagcg cggcgatgac ggggcgacgc gccatcgccg cacagcgttg
      901 tcccagcgtg gcgtcgcgcg gggagagttc cgcccgcgat agcggtcgac ggtcaaaggg
      961 aatggtcgaa tgacggaaaa gaaaagaaac ggaacggggc gaaaaggagg gcccttcggc
     1021 cgacgatgta ttggctgaat cggcagacaa ggccccacaa tccgggtcct gcacgcaggg
     1081 tagggcgcgg cttcctgcgc cgcgccacgg tcaagccttg gggtggcccc caaggccagc
     1141 ggggccgcaa tcgtggtcgc aatcacaccg aggcttcaat ccctcccctt ggattccggc
     1201 aggtaatagt ccccggtcag gcggtagtta tcccagtatt cgtgcgcgat attggggaac
     1261 gtcagtggaa caatggttgg caccgtgtct gtttttccgc catttttcgc gaatcaaact
     1321 ttacagaagg acccaatcga ggcaatcggt tgggttcttt gcttttaggg gcgggcggaa
     1381 gggcggcggg cgggacggga gagtccgcgt ccaaaacgga agcggaattc tctttcctta
     1441 tttcctgatt gtcaacggaa gaaccctctt ttttaatccg cagagacacg tccagtccga
     1501 cgcttttttg cgaatagcgg atgcctttga tcccgcttcg aacctccacg gctttttcaa
     1561 tcccaacccc gcgcgcgcaa ccccaaaggg gtgcccttcc ccctgcttcc cccgatcccg
     1621 gc
//
LOCUS       DAOQLS010000544         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_57867_length_1621_cov_3.441890, whole genome shotgun sequence.
ACCESSION   DAOQLS010000544 DAOQLS010000000
VERSION     DAOQLS010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57867_length_1621_cov_3.441890"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK362_09290"
     CDS             <1..296
                     /locus_tag="PK362_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75060.1"
                     /translation="PPQRAEYPEALYNVARVFREIKNAPEEQKAYESLAALPNKSDPY
                     RIAGLLQWGDLLVLQGKLPQAGAAYKDVVKNAADAESRGRAQEQLTALGPAAK"
     gene            331..954
                     /locus_tag="PK362_09295"
     CDS             331..954
                     /locus_tag="PK362_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC75061.1"
                     /translation="MSFIEIMKISLVMPVLLVLSIVLVAVTIERLYFYARMGQLDSRL
                     FRRIKDHLIEGRLGEAKETANRGKGLIAEALEAVLGAAAAHHKNRQEMDHVLTLYFQR
                     TQSLLGRRLGLFGTLSFISPLLGLLGTVLGVMTAFRDLAVSGQGGPTVVAAGISEALI
                     ATASGIAVAVSAALIYNYFNFRMRHLLNSLNIFGQEIAIIVTSGKDL"
     gene            964..1422
                     /locus_tag="PK362_09300"
     CDS             964..1422
                     /locus_tag="PK362_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC75062.1"
                     /translation="MHDPSQEGELEETEKINVVPLADLTLVLLIVLMVLSPMISQSMI
                     HVAVPNIKNEGAGETPKETPAETPPEPLLIAISGDGYTLNNAKVENFDALMLALDARL
                     KENTKRPVLVTAAKSIPVGRVVEVLDLAKGVGAESVSLVKSGEDLPGDKK"
     gene            1419..>1621
                     /locus_tag="PK362_09305"
     CDS             1419..>1621
                     /locus_tag="PK362_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC75063.1"
                     /translation="MSEDVRRRITGDGAIAGINIVPVIDLCLVLLVILLIISPLLEKV
                     PVEVNLPKAHAHEEKENSLSVTIS"
BASE COUNT          340 a          510 c          473 g          298 t
ORIGIN      
        1 cgccgcccca gcgggccgag tatcccgagg ccctttacaa cgtggcccgc gtcttccggg
       61 aaatcaaaaa cgctcccgag gagcagaagg cttacgagag cctggcggcc cttccgaaca
      121 aaagcgatcc ataccgcatc gcgggcctcc ttcaatgggg ggacctcctg gtgttgcagg
      181 gcaaattgcc ccaggccggc gccgcttaca aagacgtcgt gaagaacgcg gcggacgccg
      241 aaagccgcgg gcgcgcccag gaacagttga cggccctcgg accggccgcg aaatgatttt
      301 catcatcccc ggaggacatc catgccgcac atgagtttca tcgaaataat gaagatcagt
      361 ttggtcatgc cggttttgtt ggtgctgtcc attgttcttg tggccgtcac catcgagcga
      421 ctttacttct atgcccggat gggccagttg gacagccggc tattccggcg catcaaggac
      481 catttgatcg aaggacggct cggcgaagcc aaggaaaccg cgaatcgggg caaaggcctc
      541 atcgccgagg cgctggaggc cgtgctgggc gccgccgcgg cccaccacaa gaaccgccag
      601 gaaatggatc acgtcctgac cttgtacttt caacggaccc aatccctgct cggccgccgc
      661 ctgggcctct tcggaaccct gagctttatt tcgccgctcc tcggcctgct cggaaccgtg
      721 ctgggcgtca tgacggcctt ccgcgatttg gccgtgtcgg gccagggggg gccgacggtg
      781 gtcgccgccg gtatttccga agccttgatc gccacggcgt ccggcatcgc ggtggccgtc
      841 tccgccgcgc tcatctacaa ctacttcaat ttccgcatgc ggcatctcct gaacagcctg
      901 aacatcttcg gccaggaaat cgcgatcatc gtcacctccg gaaaggatct ctaaagaaaa
      961 tttatgcacg acccgtccca agaaggggaa ctggaagaaa cggagaaaat caacgtcgtc
     1021 ccgttggcgg acttgacgtt ggttcttctc atcgtgctca tggtgctttc gcccatgatc
     1081 agccagtcca tgattcacgt ggccgtgccc aacatcaaaa acgaaggcgc gggggaaacc
     1141 cccaaggaga ctcccgcgga aacgccgccc gagccgctcc tgattgccat ttccggcgac
     1201 ggctacacgt tgaacaacgc caaagtggaa aatttcgacg ccttgatgct ggccctggac
     1261 gccagattga aggaaaacac aaaacgcccc gtcctcgtga cggcggccaa aagcattccc
     1321 gtgggccgcg tggtggaagt cctggacctg gccaagggcg tcggggcgga aagcgtctcg
     1381 ctcgtcaagt cgggcgagga cctcccgggg gataaaaaat gagcgaagac gtccggcgtc
     1441 gaatcaccgg ggacggcgcc atcgccggca tcaacattgt cccggtcatc gacctttgcc
     1501 tggtgctcct ggtcatcctg ctgatcattt cgcctctcct ggaaaaggtt ccggtggagg
     1561 tcaatctgcc caaggcccac gcccacgagg aaaaggaaaa cagcctgtcg gtgaccatct
     1621 c
//
LOCUS       DAOQLS010000545         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_58586_length_1610_cov_3.098392, whole genome shotgun sequence.
ACCESSION   DAOQLS010000545 DAOQLS010000000
VERSION     DAOQLS010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58586_length_1610_cov_3.098392"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK362_09310"
     CDS             complement(<1..340)
                     /locus_tag="PK362_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC75064.1"
                     /translation="MKHRSPPVVLKSAPDSLPTMLAYLNLPVSAESYADLLAKAGKDN
                     LSHEIFLKRLLSNEVSAKFERQVQSRLRQARFPSIKTLDTFDWAHPTAIPRAKILEAF
                     ELRFVEKYEGL"
     gene            complement(337..>1610)
                     /gene="istA"
                     /locus_tag="PK362_09315"
     CDS             complement(337..>1610)
                     /gene="istA"
                     /locus_tag="PK362_09315"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNC75065.1"
                     /translation="PGPVLLRELRQQGFPGGLRILQRHLRRLREKVKPVFIRIETPPG
                     QQAQCDWAQCGSVVVGKSRRNLSAFVMVLGYSRFMYVEFTLSQCLEDFIQCHLNAFRA
                     WGGVPLKILYDNLKQVVLSRFGADIRFNPAFMEFAGVYGFQPVPCNVARGNEKGKVEN
                     GIYFLRINFLAGQTPAWPDVNKEVQLWLDETANTRIHRTTRERPVDRLAHEKPFLLPL
                     PARPYDAAVTRAVRSSHQSLVRFDGNFYSVPHKYAYKTLVLKATSTQVRLLLDCQEVA
                     AHPRSYDRGQVIEDPKHYEGILATKRQYFHTILHKRFAELGPVAKTFLDGLIAADLHP
                     IRHLQQILMLVTTYGKDEVLSAMEHAMTCNALGSAYVQNILLQRRAAKGLPELPSLEI
                     PQRPEWNTIETPDPDLSLYDEDLGPTESSPS"
BASE COUNT          348 a          384 c          552 g          326 t
ORIGIN      
        1 ccagcccttc atatttctca acaaatcgga gctcaaaagc ctcgaggatt ttggcgcgcg
       61 gaatggcggt ggggtgggcc cagtcgaagg tatcgagggt tttgatggag ggaaaccgcg
      121 cctgacgaag ccgggattga acctggcgct cgaatttggc gctgacctca ttggacagga
      181 gccgtttcaa gaagatttcg tgggacaaat tgtccttccc ggctttggcc agaagatcgg
      241 cataagattc cgcgctgacc gggaggttca aataggccag catggtgggc agggagtccg
      301 gagccgattt aagaacgaca ggcggcgagc gatgtttcat gagggagatg actccgtagg
      361 gcctaaatct tcatcgtaga gggacaaatc ggggtcgggg gtttcgatgg tgttccattc
      421 gggccgctgg gggatctcca gggagggaag ttccggcagg cctttggcgg cgcggcgctg
      481 caggagaata ttttggacgt aagcggaacc caaggcattg caggtcatgg cgtgttccat
      541 ggcggagagg acctcgtctt ttccatacgt ggtgacaagc atcaaaatct gttggaggtg
      601 ccggatcggg tggagatcgg cggcgatgag gccatccaga aatgttttgg cgacagggcc
      661 cagttcggca aaccgtttgt gaaggatggt gtggaaatac tgacgttttg tggcgaggat
      721 gccctcgtag tgcttggggt cctcgatgac ttggccccgg tcgtaggatc gggggtgagc
      781 ggcaacctcc tggcaatcca gtaaaagacg aacctgcgtg gacgtggctt tcagaaccag
      841 cgttttatag gcgtatttat gcggaacgga atagaagttg ccgtcgaacc ggacaaggga
      901 ctggtggctg gaccggacgg cacgggtgac ggcggcgtcg tagggccgcg cggggagggg
      961 aagaagaaac ggtttttcat gagcgaggcg atcgacgggg cgctcacggg tggttcgatg
     1021 aattcgggta ttggcggttt catcgagcca gagctgaacc tccttgttga cgtccggcca
     1081 ggcgggcgtt tggcccgcca ggaagttgat gcgtaggaaa tagatgccgt tttcgacttt
     1141 gcctttctca ttgccccggg cgacgttgca ggggaccggc tggaatccat aaacgccggc
     1201 gaattccatg aacgcggggt tgaaccggat atcggcgccg aaccgggaga gaacgacttg
     1261 cttcaaattg tcgtaaagga ttttaagggg gaccccgccc cacgcgcgga aggcgttaag
     1321 gtggcactgg ataaaatcct ccaggcactg ggacaaggtg aactccacat acatgaatcg
     1381 ggaatacccg aggaccataa cgaaggcgga caagttccgc cggcttttgc cgacaacgac
     1441 ggagccgcat tgggcccagt cgcactgggc ctgttgaccc ggcggcgttt cgatacggat
     1501 gaagacgggt tttacctttt cgcgcaaacg tcggaggtgc cgttgaagga tgcggaggcc
     1561 gcctggaaat ccctgctgac gcagttcccg caagagaaca ggccccggca
//
LOCUS       DAOQLS010000546         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_59122_length_1602_cov_4.210730, whole genome shotgun sequence.
ACCESSION   DAOQLS010000546 DAOQLS010000000
VERSION     DAOQLS010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59122_length_1602_cov_4.210730"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1602)
                     /locus_tag="PK362_09320"
     CDS             complement(<1..>1602)
                     /locus_tag="PK362_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNC75066.1"
                     /translation="LKSQEVLQETQQEALKRSHQQVDAAHLAHALLAVEGHIVPDILE
                     KVGVSLSKARSYIESALGRVPVVKGNTQLTTTASFREALLAAEREAKTLKDDFISPEH
                     FLLGLLEADKDFARALGLSRDQALQAMSAIRGTHRVTDENPEAKYQALEKYGRDLTEA
                     ARQGKLDPVIGRDEEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIAEGLAQRVVSGDVP
                     EGLKDKKVISLDMGALIAGAKYRGEFEDRLKAVLKEVKDAGGNVILFIDELHTIVGAG
                     ATEGAMDAANLLKPMLARGELRCVGATTLDEYKKHIEKDAALERRFQPITVGEPTVEQ
                     TIAILRGLKEKYEVHHGVRIKDGALVAAATLSHRYIADRFLPDKAIDLVDEASSRLRM
                     EIDSMPVELDEVERKLRQLEIEKRALQKESDAPSKERLDKLEKDMAELKKSSADMKKQ
                     WEVEKVAVARIRAIKGELEALKLDEQKAERGGDLGKAAELRFGKRPQLEKQMKEAQDR
                     LAGVQKDTKMLKEEVDEEDVAQVVS"
BASE COUNT          225 a          498 c          517 g          362 t
ORIGIN      
        1 ttggacacca cctgggcgac gtcttcctcg tccacctctt ccttgagcat cttggtgtct
       61 ttctgcacgc cggccaggcg gtcctgggct tccttcatct gtttttccaa ctgcgggcgc
      121 ttgccgaagc gaagctccgc ggcctttccc aaatcccccc cgcgctcggc tttttgttcg
      181 tcgagtttca acgcttccag ctcgcctttg atggcgcgga tgcgggcgac ggcgaccttc
      241 tccacctccc actgcttttt catgtcggcg gaggattttt tgagctcggc catgtccttt
      301 tcgagcttgt ccaggcgctc cttgctggga gcgtcggatt ctttctgaag ggcgcgcttt
      361 tcgatttcca gttggcgaag cttgcgctcc acctcgtcca gctccacggg catggagtca
      421 atttccatac gcaggcggct ggaggcctcg tccacgaggt cgatggcttt gtccggcaaa
      481 aagcggtcgg cgatgtagcg gtgggacagc gtggccgcgg ccaccagcgc gccgtctttg
      541 atgcgcacgc cgtggtggac ctcgtatttt tccttgagcc cccgcaaaat ggcgatggtc
      601 tgctccaccg tgggttcccc cacggtaatg ggctggaagc ggcgctccag ggcggcgtct
      661 ttttcaatgt gctttttgta ttcgtccagg gtcgtggcgc ccacgcaacg gagttccccc
      721 cgggccagca tgggtttaag caggttggcg gcgtccatgg cgccctcggt ggcgccggcg
      781 cccacgatgg tgtggagctc gtcgataaag aggatgacgt tgccgccggc gtccttcact
      841 tctttcagga cggccttcag gcggtcttca aattcccccc ggtatttggc cccggcgatc
      901 agggcgccca tgtccaggga aatgaccttt ttgtccttga gcccctcggg aacgtcgccc
      961 gagaccacgc gctgggccag gccctcggcg atggcggttt tgcccacgcc gggctcgccg
     1021 atcaaaacgg ggttgttctt ggtccggcgg gagagcacct gcaccacccg gcggatctcc
     1081 tcgtcccggc cgatgacggg gtccagtttc ccctggcggg cggcctcggt caggtcccgg
     1141 ccgtatttct ccagggcctg gtatttggcc tccgggttct cgtccgtgac gcggtgcgtg
     1201 ccgcgaatgg ccgacatggc ttgaagggcc tggtcccggg agaggcccag agcccgggcg
     1261 aagtctttgt ccgcctccag gaggccgagc aagaaatgct cgggggagat gaaatcgtct
     1321 ttgagggttt tggcctcgcg ctcggcggcc aggagggcct cgcggaagga ggcggtggtg
     1381 gtcaactggg tgttgccctt gaccaccggg acgcggccca gggcggactc tatataggaa
     1441 cgggccttgg acagggaaac cccgaccttt tccaaaatgt cggggacgat gtgcccctcc
     1501 accgccagga gggcgtgggc caaatgagcc gcgtccacct gctggtgact gcgtttaaga
     1561 gcttcctgtt gggtttcctg caggacttcc tgggatttga gg
//
LOCUS       DAOQLS010000547         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_59274_length_1600_cov_3.859547, whole genome shotgun sequence.
ACCESSION   DAOQLS010000547 DAOQLS010000000
VERSION     DAOQLS010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59274_length_1600_cov_3.859547"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1461)
                     /locus_tag="PK362_09325"
     CDS             complement(46..1461)
                     /locus_tag="PK362_09325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC75067.1"
                     /translation="MKTNDELRIAFHGGAEEVTGSRHLVEFRGTRVMMDCGLFQGHRQ
                     EALTKSQSFPAEPSTVRAVFLSHAHIDHSGGLPLLVKKGFTGPIYCTPPTADLLAIML
                     MDSAFLQEEDAKFFNKIHAAEGLRIDPLYTREDVQKTLSLLVPTDYDKSVSVVEGLEG
                     RFQNAGHVLGSAMIHLTARTAAGIRRIVFTGDLGRQSSILMNPPSSPGPVDYLLIEST
                     YGGRRHEDIGNAGDVMAEALARAEEENGPVLIPSFALERTQEIVFLLQRLLRAGRVKP
                     VNIYVDSPMASDITAIFQKYPNHTSFSKEFRTEIKGNDDPFGLKTIRYIKSVDESKAL
                     TRTPGRKIIMAGSGMCEGGRILHHLRNLVGEPSTTILLVGYQAQGTLGRRLADGAKTV
                     KIFGLPHDVGAVVRPLKHFSAHADQPDLMRFIGALSPRPRTVFLVHGDPEQREALRVA
                     LAAAGIDRVALPAFGSSTPLA"
BASE COUNT          272 a          486 c          542 g          300 t
ORIGIN      
        1 ccagtctaca tcggattttc gaacttgcca gggcttcggg gtccctcaag ccaacggggt
       61 ggaggacccg aaggcgggca gggccacccg gtcgatgccg gccgcggcca gggcgacccg
      121 cagggcttcc cgttgctccg ggtccccgtg aaccaggaac accgtgcggg ggcggggcga
      181 cagggcgccg atgaaacgca tcaggtccgg ctggtcggcg tgggcggaaa agtgcttgag
      241 cggccgcacc accgccccca cgtcgtgggg cagtccgaaa atcttgacgg tcttggcgcc
      301 gtcggccagg cggcgcccca gggtgccctg ggcctggtaa cccaccagaa ggatcgtcgt
      361 cgacggctcc cccaccagat tgcgcaggtg gtgcaggatg cgcccgccct cgcacatgcc
      421 ggacccggcc atgatgattt ttcgacccgg cgtgcgggtc agggccttgg actcgtccac
      481 ggatttgatg taacggatgg ttttcagacc gaaggggtcg tcgttccctt tgatttcggt
      541 tcgaaactcc ttggagaagc tcgtgtggtt cgggtacttt tgaaaaatgg ccgtgatgtc
      601 cgaggccatg gggctgtcga cgtagatgtt gacgggcttc acccgcccgg cccgcagcaa
      661 tcgctgcaac agaaaaacga tttcctgggt tcgctccagg gcaaagctgg ggatgagcac
      721 cggcccgttc tcctcctcgg cccgcgcgag ggcctcggcc atcacgtcgc cggcgtttcc
      781 aatgtcctcg tgccggcggc cgccgtaggt gctttcgatc agcaaatagt ccacgggccc
      841 gggggaggag ggcgggttca tcaagatgga gctttgccgc cccaggtccc cggtaaaaac
      901 gatgcggcgg attccggcgg cggtccgggc ggttaaatgg atcatggcgg accccaggac
      961 atggcccgcg ttttgaaagc gcccctcaag cccttcgacc acggaaacgg acttgtcgta
     1021 atcggtggga accaataggg acagggtttt ttgaacgtcc tcccgggtgt agagggggtc
     1081 gatgcggagg ccctccgccg cgtggatttt attgaagaac ttcgcgtctt cttcctggag
     1141 gaaagcggaa tccatgagca tgatggccag caggtccgcc gtgggcgggg tgcaataaat
     1201 ggggccggtg aacccttttt tgaccagcag gggcagaccg ccgctgtggt cgatgtgggc
     1261 gtgggagagg aacacggcgc ggacggtcga tggttcggcg gggaagcttt gggatttggt
     1321 cagggcttcc tggcggtggc cctggaacaa cccgcagtcc atcatcaccc gggtgccccg
     1381 gaactccacc aggtggcggg accccgtcac ttcctcggcg ccgccgtgaa aagcgatccg
     1441 caattcgtcg ttggttttca tgctccctcc ggaaaaaagg ataccacttt gaacgccgcg
     1501 cgcccggcgt cctcccgggc ccgggaatgc gataatgggt cttcatgaac gctccgccct
     1561 ctcccacctc cccgccggac gtcgttgttt tgggcggcgg
//
LOCUS       DAOQLS010000548         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_59571_length_1596_cov_2.874757, whole genome shotgun sequence.
ACCESSION   DAOQLS010000548 DAOQLS010000000
VERSION     DAOQLS010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59571_length_1596_cov_2.874757"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1596)
                     /locus_tag="PK362_09330"
     CDS             complement(<1..>1596)
                     /locus_tag="PK362_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75068.1"
                     /translation="AAETHEPRTAVGQTAFWGLAGLAALGLLAASTPGGQAALPAVVL
                     AGSVRSARSGTRFPTLTIPVNVLDAGSRVENGGVVSERAVAKIRRLLPDLARAGAGGV
                     YLYNGLYTPSALGALVHTVPTAGEHVLEAGAVKVLVRNYQTKRTVGTVRESGRDRAVP
                     LSDAFGNNFSVDMSRWNPAAFDATTNEARWAELAEFTAEAHRWGLRVTLDLVPWLSPD
                     ALNETNYTWAKDKFEVAGDMTDWSDERLLARYFDHALLRIVKDGKPTRVMVGHFSPGK
                     DQIDPDFSNPDYQRFLIDHLKRLIDAGVDRVRIDMAHRLGTPQKDAVWHAWTKVITEA
                     KNHAAARGRRFQFLMESYPEYGNDQWRSEFLRLYPEEQVYEVDPFHAYERMVRGEGDG
                     LNPLRDALEAARRDRNGRAVVFPSNYDEPSLRNLGGPKAAFIALLLTYERLGVPLMID
                     LREILDEEGHNIPQAGGQDAHGDNFRHPFLRQIRRGYLALRRAWTRSPHRATVESLRD
                     FFDGARRVEVLDTQYQNRFFAVGV"
BASE COUNT          270 a          504 c          537 g          285 t
ORIGIN      
        1 acgccgacgg cgaaaaaccg attttgatac tgggtgtcca acacttcgac gcggcgggcg
       61 ccgtcgaaaa agtcccgaag ggattccacc gtcgcccggt ggggcgaccg ggtccaggcg
      121 cggcgaaggg ccaggtaccc ccggcgaatt tgacgaagaa aggggtggcg gaagttgtcg
      181 ccgtgggcgt cctggccgcc ggcctgggga atgttgtggc cttcctcgtc caatatttcc
      241 cgaaggtcga tcatcagcgg aacccccagc cgttcgtagg tcaggaggag ggcgatgaaa
      301 gccgctttgg gaccgccgag gttccgaagg gacggctcgt cgtaattgct gggaaacacg
      361 acggcccgac cgttccggtc ccgccgggcg gcttccaggg cgtcgcgcag ggggttcagc
      421 ccgtccccct ctccccggac catccgctcg taggcgtgga aggggtccac ctcgtacacc
      481 tgttcctcgg ggtagaggcg aagaaattcc gaacgccatt gatcgttgcc gtattccggg
      541 taggactcca tgaggaattg aaaacggcgc ccgcgggccg cggcgtggtt cttggcctcg
      601 gtgatcacct tggtccaggc gtgccacacg gcgtcttttt ggggcgtccc cagccggtgg
      661 gccatgtcga tccgcacgcg atccacgccg gcgtcgatca gccgtttcag gtggtcgatg
      721 agaaaccgct ggtaatcggg gttggagaaa tcgggatcga tttggtcctt gcccggcgaa
      781 aaatggccga ccatcacccg tgtgggtttt ccgtctttca cgattcgaag aagcgcgtgg
      841 tcgaaataac gggccagaag gcgctcatcg gaccagtcgg tcatgtcgcc ggcgacttcg
      901 aatttgtcct tcgcccaggt gtaattggtt tcgttcaacg cgtccgggga caaccaggga
      961 acgagatcca gcgtgacgcg cagaccccaa cgatgggcct cggccgtgaa ttcggccaat
     1021 tccgcccatc gggcctcgtt tgtcgttgcg tcgaacgccg ccgggttcca gcgggacatg
     1081 tccacggaaa aattattccc gaaggcgtcc gagagcggaa cggcccgatc gcgcccgctt
     1141 tcccgaacgg taccgacggt ccgtttggtt tgataatttc gaacgaggac tttgaccgcc
     1201 cccgcttcga gcacgtgttc cccggcggtg gggacggtgt gcacgagcgc ccccagggcg
     1261 ctcggcgtgt agagcccgtt gtagaggtaa acaccgccgg cgccggcccg ggccagatcc
     1321 ggaagcaggc ggcggatttt ggcgaccgcc cgctcgctca ccaccccgcc gttttcaact
     1381 cggctgccgg cgtccaacac gttcacgggg atcgtgagcg tcgggaaccg cgttcccgag
     1441 cgagcggatc gtacgctccc cgccaggacg accgcgggca gggcggcctg ccctccgggg
     1501 gtcgacgcgg ccaggagtcc cagggccgcc agcccggcca ggccccagaa cgccgtttgt
     1561 ccgacggccg ttcggggttc gtgagtttcg gcggcc
//
LOCUS       DAOQLS010000549         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_59702_length_1594_cov_3.447693, whole genome shotgun sequence.
ACCESSION   DAOQLS010000549 DAOQLS010000000
VERSION     DAOQLS010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59702_length_1594_cov_3.447693"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="PK362_09335"
     CDS             complement(<1..105)
                     /locus_tag="PK362_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNC75069.1"
                     /translation="MFKKILIANRGEIAVRVIRACRELDIRTVAVHSDI"
     gene            complement(105..548)
                     /locus_tag="PK362_09340"
     CDS             complement(105..548)
                     /locus_tag="PK362_09340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929718.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNC75070.1"
                     /translation="MTKTAAPPSTEEQLHELLDFAAEAGLAEVVWEKEGRRVAFKRGV
                     APVVAAPAPAPVAPSAPAAPKLHVIKSPMVGTFQRAAKDRPPLVLEGSEVSAGQKMGL
                     VEAMNIPKDVLADVHGRIVKILVENGKPVEYGQPLFEVEVTPEGQ"
     gene            complement(545..1102)
                     /gene="efp"
                     /locus_tag="PK362_09345"
     CDS             complement(545..1102)
                     /gene="efp"
                     /locus_tag="PK362_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNC75071.1"
                     /translation="MIDTSEMGNGTVFEYDGQVYQIVWFQHHKPGKGGAMLRVKIRNV
                     RTGATIERTFKSGERFQELSLSRRPMQYLYAEGDNLTFMHPETFEQISVSKEKIGDSA
                     KFLVENMDVQALYLEEEFLGVELPASIELKVVSTVPGIKGDSVSNMVKPATLENNIEI
                     MVPLFIKEGEAIRVDTRTSEYVGRV"
     gene            complement(1128..>1594)
                     /locus_tag="PK362_09350"
     CDS             complement(1128..>1594)
                     /locus_tag="PK362_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNC75072.1"
                     /translation="LPHHIPGESRLAKNSPVLFDVGCRVGAYRSDLTRSFYYGKITPS
                     YKRVYGIVDAAQKAGIGTIRPGATGGQVDAAARNVIARAGYGRTFIHSTGHGVGVDIH
                     EPPWVRPKSPDLLKPNMILTVEPGIYLAGRFGVRIEDTLRVTADGRETLTRI"
BASE COUNT          279 a          468 c          512 g          335 t
ORIGIN      
        1 gatgtccgaa tggacggcga cggtgcggat gtcgagctcg cggcaggcgc ggatgacgcg
       61 gacggcgatt tcgccccggt tggcgatcag aattttcttg aacattattg gccctcgggc
      121 gtgacttcga cttcaaacaa cggctggccg tattccacgg gcttgccgtt ttccaccaaa
      181 atcttgacga tgcgcccgtg aacgtcggcc aagacgtcct tgggaatgtt catggcttcc
      241 accaggccca tcttctggcc cgcggagacc tcgctgccct ccagcaccag aggcggccgg
      301 tccttggcgg cccgttgaaa ggtcccgacc atcggcgatt tgatcacgtg gagcttgggg
      361 gccgcggggg cggacggagc gaccggggcc ggggcgggag ccgcgacgac cggggccacg
      421 ccgcgcttga aggcgacccg gcggccttct ttttcccaga cgacttcggc caggccggct
      481 tcggcggcga agtccagcaa ttcgtgaagc tgctcttcgg tggaaggggg ggcggcggtt
      541 ttcgtcatac gcgtcccacg tattcgctgg ttcgggtgtc cacgcggatg gcctcccctt
      601 ccttgatgaa caagggcacc atgatctcga tgttgttctc cagggtcgcc ggtttgacca
      661 tgttggagac ggaatcgccc ttgatgccgg gcaccgtgct cacgaccttc aattcgatgc
      721 tggccgggag ttccacgccc aaaaattcct cttccaggta gagggcctgc acgtccatgt
      781 tttctaccag gaacttggcg gaatccccga ttttttcctt ggagacggaa atctgttcaa
      841 aggtttccgg gtgcatgaag gtcaggttat cgccctcggc gtagaggtat tgcatggggc
      901 gccgggagag cgaaagttct tgaaagcgtt cgccggactt gaaggtgcgc tcgatggtgg
      961 cgccggtgcg cacgttgcgg attttgaccc gcaacatggc cccgcccttc ccgggtttgt
     1021 gatgctggaa ccaaacgatt tgatagacct ggccgtcgta ttcaaagacg gtgccgttgc
     1081 ccatttccga tgtatcgatc atgtcgtgct ccgttgaaag ggatgaacta gatacgggtt
     1141 aacgtttccc gaccgtccgc cgttacgcga agggtgtcct cgatccgaac cccgaagcga
     1201 ccggccaggt aaatgcccgg ttccacggtg agaatcatgt tcggttttag gaggtcggga
     1261 cttttggggc gaacccaggg cggttcgtgg atgtccacgc ccaccccgtg ccccgtgctg
     1321 tggatgaagg tacgcccgta acccgcccgg gcgatgacgt tccgcgcggc ggcgtccacc
     1381 tggccgcccg ttgcgccggg ccggatggtg ccgattccgg ccttttgggc ggcgtccaca
     1441 attccataaa cccgtttata agacggcgtg attttaccgt aataaaacga ccgggtcaag
     1501 tcggagcggt aggcccccac ccgacagccc acgtcaaaca aaaccgggct gttcttggcc
     1561 aaacgggact ccccggggat gtggtggggc aggg
//
LOCUS       DAOQLS010000550         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_59843_length_1592_cov_3.720885, whole genome shotgun sequence.
ACCESSION   DAOQLS010000550 DAOQLS010000000
VERSION     DAOQLS010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59843_length_1592_cov_3.720885"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..485
                     /locus_tag="PK362_09355"
     CDS             12..485
                     /locus_tag="PK362_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75073.1"
                     /translation="MDNIKIRIKSGNNEFEAEGPSAEVEKHMEIWRREFSGLAKSPES
                     ADPSNAYYFDPGRDGLTLRKAPGIGPKGRKLADALLLILHGYRELQAKTQVEATRIAR
                     DLRESGYNDLAKLARSYHRLQKEGLAVRSGRGKGTRYRLTEPGQNAASTLARSFF"
     gene            complement(803..1096)
                     /locus_tag="PK362_09360"
     CDS             complement(803..1096)
                     /locus_tag="PK362_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75074.1"
                     /translation="MRREGGAKGTAFDGGAAATPRRSWIAFQRDLGYGAMAGSGFSEV
                     GGRPGVVLFGFGSALNLVFVFFVELPNVIDVIQDQLRFGQTEGQSEAVRFILL"
     gene            complement(1352..>1592)
                     /locus_tag="PK362_09365"
     CDS             complement(1352..>1592)
                     /locus_tag="PK362_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011653851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNC75075.1"
                     /translation="IARALAMRPRAVLFDEPTSSLDPEMKEDVVDVMSGLARDGLTMV
                     IVTHEPAVVNRIATRILRFGPLGRIEEDRRGPAHP"
BASE COUNT          420 a          446 c          425 g          301 t
ORIGIN      
        1 ggtgaattcc tgtggacaac atcaaaattc gcatcaagtc cggcaacaac gagttcgagg
       61 cagagggacc cagcgccgaa gtcgaaaaac acatggaaat atggcgccgg gagttctcgg
      121 gcctggccaa atcgccggag tccgccgacc ccagcaacgc ctattatttc gaccccggac
      181 gggacggact gacccttcga aaagcgcccg gaatcggccc gaagggccga aaattggccg
      241 acgccctgct cctcatcctc catggctacc gcgaacttca ggccaaaacc caggtggagg
      301 ccactcgaat cgcccgggac ctgcgcgaat ccggatacaa cgacctagcg aaattggccc
      361 gctcctacca ccgccttcag aaggaagggc tggccgtgcg ctccggacgg ggaaaaggaa
      421 cccgttaccg actgacggag ccgggccaaa acgcggcgtc cacgctcgcc cgctctttct
      481 tctaaaaaat tccaatcacc gatgcaaagc caacaatcga cccaaccgcc ttattccaac
      541 tattaacttc tgataatata tattatgtta aataacagac aaaacacgac gacccaaatc
      601 aagttatatt ctgtgttaaa taaattctct aaacatatga aaaacaattg ttttgtggtt
      661 gattctcaaa taactttgta tttattttga cttagcactc ttaatttgta ttatttcaac
      721 tgattttagc gttttcgctt attattccag gaggctccgc gtgttcacaa ttggtgaatt
      781 caagggaaat atggttatca gtttaaagca aaatgaatcg gaccgcttcg gattgacctt
      841 cggtttgacc aaagcgaagt tgatcctgga tcacatcgat gacattcgga agttctacga
      901 agaaaacaaa gacaagatta agcgccgaac cgaagccgaa taaaaccacc ccgggacggc
      961 caccgacctc ggaaaaaccg gatcccgcca tcgcgccata acccaggtcg cgctgaaaag
     1021 cgatccacga ccggcggggg gtggcggccg cccccccatc aaacgccgtg cccttggcgc
     1081 cgccctcacg ccgcattccc cccgccttaa aaaaatagac ctcaggacga ccattggacc
     1141 ttcccgcgaa cggggggggt cgaaagtcga tgaaggcctt tagcgacccc aaggaaaatt
     1201 caaggggtgt ggctggaggg acctgttata aggaaggaaa aggttaacga aaagccaggg
     1261 agggcgaaat ggggaatgga aagggcgaag gccttggaaa aagggtggaa gccgcagggg
     1321 gcaaggcccc gtttaagagt ttttgaccgc tttaggggtg ggcggggccg cggcgatcct
     1381 cctcgatccg ccccaggggg ccgaagcgca aaatccgagt ggcaattcgg ttgaccaccg
     1441 ccggctcgtg ggtgacgatg accatggtca aaccgtcccg ggccaacccg gacatcacat
     1501 ccaccacgtc ttccttcatt tccgggtcga ggctggaggt cggctcgtcg aatagaacgg
     1561 cccgggggcg catcgccagg gcccgggcga tg
//
LOCUS       DAOQLS010000551         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_59911_length_1591_cov_3.972005, whole genome shotgun sequence.
ACCESSION   DAOQLS010000551 DAOQLS010000000
VERSION     DAOQLS010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59911_length_1591_cov_3.972005"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1591
                     /locus_tag="PK362_09370"
     CDS             <1..>1591
                     /locus_tag="PK362_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75076.1"
                     /translation="PLFVRGAEALGRWMGARGLINGETGKPWTAEDMGRLYIRNAWIE
                     GIFLPGLFLAGLNLVDGLVPGVREAVPSMLWALVPHLAFVLLHYFPRRFTAWLGGKYG
                     VVPATRSMVYIGAGYLLGVDFYLYSFTSPFAGWTLLLSGLVWGTALVFHARMARGIAR
                     PLEVRSFTDSPEDAARAAWLESILRGAGQRPEPSPWTPELSAVGERRWPAESAALGPP
                     VRSWIKILFPERFKAWERISLESPPGMDRAFVAAVRLRDLNRDLLRQGLYATYRAFLI
                     EGQPAVDYELKKVEEARRFRLNGRDLTLVALAPLKARRVPAGPAEFLNPPAGLLVAEP
                     GRFRGAAEKVVSSMMPVGAPFTEAPALAAAVQRMIRRDLDELFRTPAESSKLDMIASG
                     VRAFYQLRGKLPDGMTLAGDAQWNREMDAMLTFLAAAQAKTYEIVLLSEAAYGYGRDS
                     AFPLLNGIAEGTLPRIDLVYLLMNISSDRTSWKALGDLHREFLGKPMSEDPPYLDQIV
                     PLFNRLATHDTPALREGVRRVM"
BASE COUNT          247 a          512 c          512 g          320 t
ORIGIN      
        1 cccgctgttc gtccgggggg ccgaggccct ggggcggtgg atgggcgccc ggggcctgat
       61 caacggggaa acgggaaagc cctggaccgc cgaggacatg ggccggcttt atattcgaaa
      121 cgcgtggatc gagggaattt tccttcccgg gctctttttg gcggggctca acctggtcga
      181 cggcctggtc ccgggagtcc gggaggccgt gccgtccatg ctctgggccc tcgttccgca
      241 cctcgccttc gtgctcctcc attatttccc ccgccggttc acggcctggt tggggggaaa
      301 gtacggtgtg gttccggcca cccgttcgat ggtttatatc ggcgccggtt atttgctggg
      361 cgttgatttc tacctctaca gttttacctc gcccttcgcc ggatggacgt tgctcctgag
      421 cggactggtg tggggaaccg ccctggtctt tcacgcccgg atggcccggg gcatcgcccg
      481 gcccctggaa gtgcgctcct tcacggattc gccggaggac gccgcccggg ccgcctggct
      541 ggagtcgatt cttcgaggcg cgggccagcg cccggaaccg tctccctgga cgcccgaatt
      601 gtccgccgtc ggggaacggc gttggccggc cgagtcggcc gccctgggcc cccccgtccg
      661 atcgtggatt aaaatattgt tcccggaacg tttcaaagcc tgggaacgaa tctccctgga
      721 atccccgccg gggatggacc gggcctttgt ggccgccgtc cgccttcggg acctcaatcg
      781 cgacttgctc cgacagggac tctacgcgac ttaccgcgct tttctgatcg agggtcaacc
      841 ggcggtggat tacgaactta aaaaggtgga ggaggctcgc cggtttcgat tgaacggccg
      901 ggacctgacc ctcgtcgcct tggccccctt aaaggcgcgg cgggtccccg cgggacccgc
      961 ggaatttctc aatccgcccg cgggcttatt ggtggcggag ccgggacgat ttcgaggcgc
     1021 tgcggaaaaa gtcgtatcga gcatgatgcc cgtgggcgct cccttcaccg aagccccggc
     1081 cctcgcggcg gcggttcaac ggatgatccg acgggacttg gacgaactct ttagaacgcc
     1141 ggccgaatct tccaagttgg atatgatcgc ctcgggcgtt cgggctttct atcaattaag
     1201 aggcaaattg ccggatggaa tgaccctggc cggcgacgcc caatggaatc gggaaatgga
     1261 cgccatgttg accttcctgg cggcggctca ggccaaaacc tacgaaatcg tcctgttgtc
     1321 cgaggcggcc tacggttacg gccgggacag cgccttcccg ctcctgaacg ggatcgccga
     1381 aggaaccctc ccccggatcg atttggttta cctgcttatg aacatctcct cggatcggac
     1441 ttcctggaag gccctgggcg acctccaccg ggagttcctc gggaaaccga tgtccgagga
     1501 ccccccctat ctggaccaaa tcgtgccgct cttcaaccgt ctggcgaccc acgacacgcc
     1561 ggcgcttcgc gagggcgtgc gccgggtcat g
//
LOCUS       DAOQLS010000552         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_60352_length_1585_cov_4.768627, whole genome shotgun sequence.
ACCESSION   DAOQLS010000552 DAOQLS010000000
VERSION     DAOQLS010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60352_length_1585_cov_4.768627"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK362_09375"
     CDS             complement(<1..304)
                     /locus_tag="PK362_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75077.1"
                     /translation="MKKWILMATVLAGTTAQAAWFGQIGRANGRDEHSIEFIYDTGDR
                     DIRVKGTNGPDVQLNAHRFTVQYARGLGQGFELFARGVPFTGRINLENSSFNPDLWG"
     gene            complement(301..702)
                     /locus_tag="PK362_09380"
     CDS             complement(301..702)
                     /locus_tag="PK362_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75078.1"
                     /translation="MIRFDYSVFREGKAPVIPVEFWGRGRWHNLWVYVDSGASFTILH
                     TYEASRLGVDLRKCPKFYIVVAGDRRIPVFTRTLKLRLDRTVVPVEVGFCSALGGAFN
                     LLGRKGVFDRFKVCFDDRNETVSFNKGGRRR"
     gene            complement(671..901)
                     /locus_tag="PK362_09385"
     CDS             complement(671..901)
                     /locus_tag="PK362_09385"
                     /inference="COORDINATES: protein motif:HMM:NF039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HNC75079.1"
                     /translation="MNKNDRWISKNFGKLVDLYGGRYVAVADERVVAVGARPEAVERK
                     ASRRPGLPASVVRVPSLGQGPALDPLRLFRLS"
     gene            complement(910..>1585)
                     /gene="atpG"
                     /locus_tag="PK362_09390"
     CDS             complement(910..>1585)
                     /gene="atpG"
                     /locus_tag="PK362_09390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNC75080.1"
                     /translation="LTHPLLKGAPAGPRGVLLISADRGLCGAFNTNLIKKTLEFLRNN
                     TDRDVRLFCVGRKGRDYFRRAGLALGGEYVGVFGQLGYATAEVIGQDLMNFFTKGGAA
                     DVTLIYTEFKSAIQQNVVVAPLLPLAPPARPVATRWEAPFIYEPERSRLLESLFPRVL
                     KAQIFRALLESYASEMGARMTAMENASKNAKELIAGLTLTANKIRQAAITKEISELVG
                     GAEALN"
BASE COUNT          320 a          534 c          453 g          278 t
ORIGIN      
        1 acccccacag atcgggattg aaggacgaat tttccaaatt gattcgcccg gtgaagggca
       61 cgccccgggc gaagagttcg aacccctgac ccaaaccgcg ggcgtattga accgtgaacc
      121 ggtgcgcgtt cagttgaacg tccgggccgt tggtgccttt gacccggatg tcccggtcgc
      181 cggtgtcgta aatgaattcg atcgaatgct cgtcccgccc gttggcgcga ccgatctgcc
      241 caaaccacgc cgcctgggcg gtggtgcccg ccaacaccgt ggccatcaaa atccattttt
      301 tcatcgacgt ctgccccctt tgttgaacga cacggtttcg ttccggtcgt caaaacatac
      361 cttaaatcgg tcgaacacgc ccttgcggcc cagcaaattg aaggcgccgc cgagggcgct
      421 gcaaaaaccc acttccacgg gaaccaccgt ccggtccagc cgcaacttca gcgttcgggt
      481 gaaaaccggg atgcgccgat cgcccgccac gacgatgtaa aacttgggac atttccgcag
      541 atccaccccg aggcggctcg cctcgtaggt gtggagaatg gtgaaactcg cccccgaatc
      601 cacgtacacc cacaggttgt gccaccggcc ccgcccccaa aactcgacgg ggatgacggg
      661 ggccttgccc tcacgaaaga cggaatagtc gaagcggatc aagcgccggt ccctgcccca
      721 acgacggaac gcgaaccacc gacgccggaa gacccggacg gcgggacgct ttccgttcca
      781 ccgcctcggg gcgggcgccc accgccacca cgcgctcgtc ggccacggcc acgtaacgac
      841 cgccgtaaag gtccacgagc ttgccgaaat tcttcgaaat ccagcggtcg tttttgttca
      901 tggcgtcccc tagttcaacg cctccgcgcc gcccaccaac tccgaaattt ccttggtgat
      961 ggccgcctgg cgtattttat tggccgtgag cgtcaaaccg gcgatcagtt ccttggcgtt
     1021 tttcgaggcg ttttccatgg ccgtcatgcg ggcgcccatt tccgaagcgt aggattccaa
     1081 gagcgcccgg aaaatttgcg ctttgagcac ccggggaaac agggattcca acaggcgcga
     1141 acgctccggc tcgtagataa aaggcgcctc ccagcgggtc gcgaccggcc gcgccggcgg
     1201 cgcgaggggc agaaggggcg ccacgaccac gttctgctgg atggcggatt taaattcggt
     1261 gtaaataagg gtgacgtcgg ccgcgccccc cttcgtaaaa aaattcatga gatcctggcc
     1321 gatgacctcc gccgtggcgt agcccaactg gccgaacacg cccacgtatt ccccccccag
     1381 ggccaacccg gcccggcgga aataatcccg accctttcga cccacgcaaa agaggcgtac
     1441 gtcccggtcg gtgttgttcc ggaggaactc cagggttttc ttgatcaaat tcgtgttgaa
     1501 ggcgccgcag agcccccggt ccgccgagat caacaacacc ccccggggcc ccgccggcgc
     1561 gcccttcaga agcgggtgcg tcaat
//
LOCUS       DAOQLS010000553         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_60360_length_1585_cov_3.988889, whole genome shotgun sequence.
ACCESSION   DAOQLS010000553 DAOQLS010000000
VERSION     DAOQLS010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60360_length_1585_cov_3.988889"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..917
                     /gene="rfbD"
                     /locus_tag="PK362_09395"
     CDS             45..917
                     /gene="rfbD"
                     /locus_tag="PK362_09395"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNC75081.1"
                     /translation="MKVLLFGGAGQLGTDLRGTKPASVDLFCPPHPEADIAERSSVAT
                     FLNRHSPDAVVNAAAFNLTDQCEINPEIATRANADGPAVLAALCRERGVRLLHFSTDY
                     VFDGTKPTPYVESDAPHPLSQYGRSKREGEKAVLGASPDFWVVRSSSLFGRAGSRGLG
                     GNFVESILARARSGAPLTVVNDVRMSPTYTRDLAERTWQLLGRGVPGGVYHVANDGSC
                     TWFEFAVEILRIAGARADVLPLRSDQWPAKFSRPAQSALASERAAALGFPPMRPWPEA
                     LRDYLAAPRSPEKL"
     gene            950..1192
                     /locus_tag="PK362_09400"
     CDS             950..1192
                     /locus_tag="PK362_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC75082.1"
                     /translation="MPTEPRPTREVMDIRELASYLGIGKSKIYGLIRARKIPAARIGR
                     QYRFSKDVIDAWLRESLITQPPEPQRGLFGGPSGPA"
     gene            1305..1526
                     /locus_tag="PK362_09405"
     CDS             1305..1526
                     /locus_tag="PK362_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75083.1"
                     /translation="MAQKVAKAGVKREEGFLYFIDKQGDVSRAKMARGGKKGGKPQKV
                     AKVGIKKEKGFLYFLDKQGDVSRAKMVRR"
BASE COUNT          287 a          519 c          501 g          278 t
ORIGIN      
        1 ggggtggctc aaaccctccg ttcccgccgg ccgctgaccc gcgcgtgaag gtcctcctct
       61 tcggcggcgc gggccagctg gggacggatt tgcgcggcac gaagcccgcg tccgtcgatc
      121 ttttttgccc tccgcatccc gaggccgaca tcgccgaacg atcgtcggtc gcgacctttt
      181 taaaccgaca ttccccggac gcggtggtca acgccgccgc gttcaatctg accgaccagt
      241 gtgaaatcaa cccggaaatc gcgacgcggg ccaacgccga cggccccgcc gtgctggcgg
      301 ctttgtgccg cgaacggggc gttcgcctcc tccatttcag caccgactac gtgttcgacg
      361 gcacgaaacc cacgccctac gtggagagcg acgcgcccca ccccctgagc cagtacggac
      421 gatcgaaacg cgagggggaa aaggccgtgt tgggggcctc cccggatttc tgggtggtgc
      481 gatcctcgag ccttttcggc cgggcgggct cccgggggct gggggggaac ttcgtggaat
      541 ccattttggc ccgggcgcgc tcgggagcgc ccctcaccgt cgtcaacgac gtccgcatgt
      601 ccccgaccta cacccgggat ttggccgagc ggacctggca actcctggga cggggcgttc
      661 cggggggcgt ttaccacgtg gccaacgacg ggtcctgcac ctggtttgaa ttcgccgtcg
      721 aaatccttcg gatcgccggg gcgcgcgccg acgtgcttcc gttgcgctcc gatcaatggc
      781 ccgccaaatt ttcccgcccc gcccaatcgg ccctggccag cgagcgcgcc gccgccctcg
      841 ggtttccgcc gatgcgcccc tggccggagg cccttcggga ctacctcgcc gcgccccgtt
      901 cccccgaaaa gctttgactt ccgtcggagc tttttatata aaaaccccga tgccgacgga
      961 accgcgcccc acccgggaag tgatggacat tcgggaactg gccagctacc tgggaattgg
     1021 aaaatccaaa atttacggtc tcatccgcgc ccggaaaatt ccggcggcgc ggatcggacg
     1081 tcaataccgg ttttcaaagg acgtgatcga cgcctggctg cgcgaaagcc tcatcaccca
     1141 gccgcccgag ccccaacggg ggttgttcgg gggtccgtcc ggccccgcgt aaggcccccg
     1201 tcgatccgcg cgggggcttc cgacggaaac cgtcttgcaa ttcgcggagg atttgctaaa
     1261 gttttgacac gcagcgttat ttctcgacga aggaggtgaa acacatggca caaaaagtgg
     1321 cgaaagccgg cgttaagcgc gaagaggggt tcctttactt catcgacaag cagggggacg
     1381 tttcccgcgc gaagatggct cgcggtggaa aaaaaggcgg caaaccccag aaggtcgcga
     1441 aggtggggat caagaaagaa aaaggcttcc tgtacttcct cgataagcag ggagacgttt
     1501 cccgcgcgaa aatggtccgc cgttaaaagc ggattggccc cgttgcaggg gtcgcagccc
     1561 gtggacacgg tttgcggggc gttgc
//
LOCUS       DAOQLS010000554         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_60465_length_1584_cov_2.934598, whole genome shotgun sequence.
ACCESSION   DAOQLS010000554 DAOQLS010000000
VERSION     DAOQLS010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60465_length_1584_cov_2.934598"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>1584
                     /locus_tag="PK362_09410"
     CDS             149..>1584
                     /locus_tag="PK362_09410"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNC75084.1"
                     /translation="MALTPALPHRRARRVALASGRGLVVALFLLFLNLAAGRFSFRWD
                     GTGDRRYSLAPATRRLLEGLDDPVRVTFYLTPDLPQPHAARGRFVSDFLEEWRVAGRG
                     RLIPEVVHPDGSDAVNRDLRAANLVPTGFTEIADDRYRVREGYMAIVLRYRDRTEILP
                     YVKDSLGLEFDLAARLRSLVSPRRKTLLLIGGHDELTAEALKAGPGAGLFSEFTVESA
                     PLSTETGVSPDALLLLGPKRTLEAREIDVLDLFLSSGVPVIVAFNHRSINPKGFRSFP
                     IDTGLGALLAHYGVRSDPAFVMDLQCQTLGFQNAQGAFRARYWPFVSVTLWNPDHPAT
                     RNLDEAVFPFCHPLRPTGDALKTSRWTTLARSSFDSWTFDGNVTLDPVALPLLHNART
                     SGPFTLAALVEGSTTSFSPPARRVPRFSLSVLGTAFLADPRAPHPAGNELFLVQLLQW
                     VTRDTDLGDLPRKGAAQRPLRPAGRVAR"
BASE COUNT          210 a          557 c          488 g          329 t
ORIGIN      
        1 cggtcggcgg ggggctcgct ttcctgggcg ttgacgggca tttcgattcc tttcgccgcg
       61 gcgtgatcga cgctcgcgac gtcctttact tcttgtccgg aacggcggcg ggtttgctcg
      121 gcgcgtacgc aggcgtggcc ggtcgccgat ggcgttgacc ccggcgcttc cccaccggcg
      181 cgcccggcgc gttgccctgg ccagtgggcg cggcctggtc gtcgcgctgt tcctcctgtt
      241 tctcaatctg gcggcgggac gtttttcctt tcgctgggac gggaccggcg accggcgcta
      301 ctccttggcc ccggccaccc gacgcctctt ggaagggttg gacgatccgg tccgcgtcac
      361 cttctacctc accccggacc tcccccaacc ccacgcggcc cgggggcggt ttgtgtcgga
      421 ttttcttgag gaatggcgcg tcgccggacg cgggcgactg atcccggagg tcgtgcatcc
      481 cgacgggtcg gacgcggtga accgggacct ccgggcggca aacctggtgc cgaccggatt
      541 caccgaaatt gccgacgacc gctaccgcgt ccgcgaaggg tacatggcga tcgtcctgcg
      601 ctatcgggac cggacggaaa tcctccctta cgtgaaggac tccctcggct tggaattcga
      661 tttggcggcg cgccttcggt ccctggtttc tccccgccgc aaaaccctgc ttctgattgg
      721 cggacacgac gagttgaccg cggaggcctt gaaagccggt cccggcgccg gcctgttctc
      781 cgaattcacc gtggaatcgg cgcctctgag cacggaaacg ggcgtttcgc cggacgccct
      841 gcttcttctc ggccccaagc gaaccctcga agccagggag atcgacgtct tggatttatt
      901 tctttcaagc ggagtgccgg tcattgtggc cttcaaccat cggtccatca accccaaagg
      961 cttccgatcg ttccccatcg acaccgggct gggggcgctc ttggcccatt acggcgtccg
     1021 gtccgatccg gccttcgtca tggatcttca atgccaaacg ttggggtttc aaaacgccca
     1081 gggggccttt cgcgcccgtt attggccctt cgtttcggtg acgctgtgga accccgatca
     1141 ccccgcgacc cggaacctcg acgaagccgt gtttcccttc tgccatcccc ttcggccaac
     1201 gggcgacgct cttaaaacct cccgctggac gacgttggcg cgttcgtcct ttgacagttg
     1261 gacctttgac gggaacgtca cgctggaccc ggtggcgctg cccctattgc ataacgctcg
     1321 aacgtccgga ccgttcaccc tggccgcctt ggtcgagggg tccacgacgt ctttctcccc
     1381 gccggcgcgg agggtccccc gcttctccct ctccgttttg ggaacggctt tcttggcgga
     1441 tccccgcgcc ccgcatcccg ccggcaacga gctttttctt gtccaacttc tgcaatgggt
     1501 cacccgggac accgacctgg gcgatctccc tcggaagggg gccgcgcagc gccccctgcg
     1561 cccggcgggt cgggtcgccc gatg
//
LOCUS       DAOQLS010000555         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_60689_length_1581_cov_5.041284, whole genome shotgun sequence.
ACCESSION   DAOQLS010000555 DAOQLS010000000
VERSION     DAOQLS010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60689_length_1581_cov_5.041284"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..>1581
                     /gene="ispG"
                     /locus_tag="PK362_09415"
     CDS             56..>1581
                     /gene="ispG"
                     /locus_tag="PK362_09415"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNC75085.1"
                     /translation="MKSAVPPTPKAPVFSPDLFAYRRRKTRVVKVGDVGVGGDHPLRL
                     QSMTTTRTQDTDATVQQTVRLVQAGCEIVRITAPTEVDAKNLKHIAAALRAKGVRVPL
                     VADIHFRPEAALEAAEHVEKVRINPGNFADGKMFKTREYSDREYAEELERIEERFTPL
                     VLKMKKLGRALRIGTNHGSLSDRIMNRYGDSPEGMVESALEFLAICRRNNYHDVIFSM
                     KSSNPKVMIAAYRLLAARLDAQGWDYPFHLGVTEAGDGEDGRIKSAVGIGALLEDGIG
                     DTLRVSLTEDPEFEIPVARRLAARFDGRPAVRYAPRRRDGDAELNPLGDAYHYQRRAA
                     RETAVGPIRLGGNHPPRVVTDISALGLSGSDILAALEKALKNSKQIPPEILVQDVSMP
                     EDAGNLSNLKRDLKFETSRLGFLARLTGTEDVAANALDAADGLIVPLKTGPSASFLAA
                     VARAATDAGKSLWLEATDAGELLAAAQNLGERPFNVVLSLRSTDASRLLHQWRFVAAQ
                     P"
BASE COUNT          299 a          573 c          464 g          245 t
ORIGIN      
        1 tccacggggg attccgaccg gcggcgatcg acgggggttt ggtataatga cccccgtgaa
       61 atccgccgtt cctccgacgc ccaaagcgcc cgtcttttct cccgatctct tcgcctaccg
      121 ccgccgcaaa acacgggtgg tgaaagtggg cgacgtgggc gtcggcgggg accaccccct
      181 tcgcctccag tccatgacga ccacccgcac ccaggacacc gacgcgacgg tccagcagac
      241 ggttcgactg gtccaggccg gatgcgaaat cgtccgcatc accgccccga ccgaagtgga
      301 cgccaaaaat ctcaaacaca tcgccgcggc gctccgcgcc aagggcgtcc gcgtccccct
      361 cgtcgccgac atccatttcc gccccgaggc cgccttggaa gccgcggagc acgtggagaa
      421 agtccgcatc aaccccggca atttcgccga cggcaaaatg tttaaaaccc gggaatattc
      481 cgaccgggaa tacgccgagg aactggagcg gatcgaggag cggttcaccc ccctggttct
      541 caaaatgaaa aaactcggcc gggcgctccg catcggaacc aaccacggat cgctctcgga
      601 ccgcatcatg aaccgctacg gcgactcccc cgaaggcatg gtggaaagcg ccctggaatt
      661 tttggccatt tgccggcgaa acaactacca cgacgtgatt ttctccatga agtcctccaa
      721 ccccaaagtg atgatcgccg cctaccgcct gttggcggcc cggctggacg cccaggggtg
      781 ggactacccg ttccacctgg gcgtgaccga agcgggcgac ggcgaggacg gccgcattaa
      841 atcggccgtg ggcatcggcg ccctgctgga ggacggcatc ggcgacaccc tgcgggtctc
      901 cctcacggag gaccccgaat ttgaaatccc cgtggcccgc cggctggccg cccgtttcga
      961 cggacgtccg gccgtccgct acgccccccg ccggcgggac ggcgacgccg aattgaatcc
     1021 cctgggcgac gcctaccatt accaacgccg cgccgcccgg gaaaccgcgg tggggccgat
     1081 ccggctgggc ggcaatcacc ccccccgggt cgtgacggac atttcggccc tcggcctctc
     1141 gggatcggac attttggccg ccctggaaaa agcgctgaag aattccaaac agatcccccc
     1201 ggaaatcctg gttcaggacg tatcgatgcc ggaggacgcg gggaatctct cgaatttgaa
     1261 aagggatctg aaatttgaaa cctcgcgcct cgggttcctg gcccggctca cgggcaccga
     1321 agacgtggcc gccaacgccc tcgacgccgc cgacggattg attgttccct tgaaaaccgg
     1381 cccctcggcc tccttcctgg cggccgtcgc ccgggcggcc accgacgcgg gaaaatccct
     1441 ttggctcgaa gccaccgacg ccggggagct cctcgcggcg gcccaaaacc tgggcgaacg
     1501 ccctttcaac gtcgtcctgt cccttcgaag cacggacgcc tcgcgcctcc tccaccaatg
     1561 gcgcttcgtc gccgcccaac c
//
LOCUS       DAOQLS010000556         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_60860_length_1579_cov_3.398294, whole genome shotgun sequence.
ACCESSION   DAOQLS010000556 DAOQLS010000000
VERSION     DAOQLS010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60860_length_1579_cov_3.398294"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PK362_09420"
     CDS             <1..1112
                     /locus_tag="PK362_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HNC75086.1"
                     /translation="YYIFAEQITVRRGDARFSDWESLRGRRVGTLTASLAQTLMERDG
                     RMTPVAYPSPVEIYKDLELGRTDAVLLDVPIAAWYAGPNPRLQNVGEPVGRGLYAGGI
                     RKDSPEFRRRVDDALAVLIRNGTLETIYRKWNMWTKDQSGLKDALATAPSIPDRGSVR
                     RYLGLVFRGAGTTILISVLSMALAVVVGFLICLGRLHGGRWLRAACVGYVEITRGTPL
                     LIQLYVLYYGLPNLGLQLNALVAAVLGVGFNYAAYESEIYRAGILSVPRGQEEAARSL
                     GLTTWQTLRHVVLPQAIRTVLPPSTNDFIALFKDTSLVSIITVMELTRAYSQAATATF
                     RFLELGLLTAGLYLMMSLPLSFLSRRLESQRRALH"
     gene            1099..>1579
                     /locus_tag="PK362_09425"
     CDS             1099..>1579
                     /locus_tag="PK362_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC75087.1"
                     /translation="MLCIDVQHLSKSFGDHPVIDDLSLRVEEGEVVVVQGPSGVGKST
                     FLRCLTYLEPFQRGTVRVGEIEIGAGMDEKRHHDVIRHLREQMGFVFQFFNLFPHLTV
                     LQNLTLAPLRVQNRPVEEAEKEAMALLNRVGLGGKAAAYPESLSGGQKQRVGIARALA
                     "
BASE COUNT          270 a          466 c          512 g          331 t
ORIGIN      
        1 cctattacat tttcgccgaa cagatcaccg ttcgacgggg cgacgcccgt ttttccgatt
       61 gggaaagcct tcggggccgc cgggtgggca cgctgacggc ttccctggcc caaaccctga
      121 tggagcggga cgggcggatg acgccggtcg cctatccatc gcccgtcgaa atctacaagg
      181 acctcgaact gggccggacc gacgcggtgc tgttggacgt gcccatcgcg gcctggtacg
      241 cgggcccgaa cccgcgcctg caaaacgtgg gggagcccgt ggggcgcggc ctttacgcgg
      301 ggggaattcg aaaagactcc ccggaatttc ggcggcgcgt ggacgacgcc ctcgccgttt
      361 tgattcggaa cgggacgttg gagaccattt accgcaagtg gaacatgtgg accaaagacc
      421 aaagcggttt gaaggacgcc ttggcgacgg ccccgtccat ccccgaccgg ggatccgttc
      481 gacggtattt gggcctggtg ttccgcgggg cgggcacgac gatcctgatt tcggtgctat
      541 ccatggcgct ggcggtggtg gtggggtttt taatttgttt gggccgactc cacggcggcc
      601 gatggcttcg ggccgcttgc gtggggtacg tggaaatcac ccggggaacg ccgctcctga
      661 tccagctcta cgtgctttac tacgggctgc cgaaccttgg cctgcaactg aacgccctgg
      721 tggcggccgt cctgggggtg gggttcaatt acgcggcgta tgaatcggaa atttaccggg
      781 ccgggattct gtccgttccc cgggggcagg aagaggcggc gcgttccctg ggcctcacga
      841 cctggcaaac cctgcggcac gtggtcctgc cccaggcgat ccgcacggtg cttccaccgt
      901 cgacaaacga tttcatcgcg ctttttaagg acacctccct ggtgtccatc atcaccgtca
      961 tggaattgac ccgggcctat tcccaggcgg ccacggcgac ctttcgattt ttggagcttg
     1021 gattgctcac ggccgggttg tatttgatga tgagccttcc cctctccttt ttgtctcgcc
     1081 gattggagtc ccaacgacgt gctttgcatt gacgttcaac atctttcgaa atccttcgga
     1141 gaccacccgg tcatcgacga tctgtcgctg cgggtggagg agggggaggt ggtcgtcgtt
     1201 caggggccct cgggggtggg gaaaagcacc ttcctgcgct gtttaaccta tctggagccc
     1261 tttcaacggg gcactgtgcg ggtgggggag atcgaaatcg gcgccggcat ggacgaaaaa
     1321 cgccaccacg acgtgatccg ccatttgcgg gagcagatgg gattcgtttt tcaatttttc
     1381 aaccttttcc cgcacctgac ggttcttcag aacctgaccc tggcgccgct tcgcgtgcag
     1441 aatcggcccg tcgaagaagc cgaaaaggaa gccatggctt tattgaaccg cgtggggttg
     1501 ggcggaaagg ccgcggctta tccggagtcg ctctccggcg gacaaaaaca acgggtgggc
     1561 atcgcccggg ccctggcca
//
LOCUS       DAOQLS010000557         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_60999_length_1577_cov_3.057162, whole genome shotgun sequence.
ACCESSION   DAOQLS010000557 DAOQLS010000000
VERSION     DAOQLS010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60999_length_1577_cov_3.057162"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1577
                     /locus_tag="PK362_09430"
     CDS             <1..>1577
                     /locus_tag="PK362_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75088.1"
                     /translation="LTVEIPALLLGSVALGPLGALAGLAVFTFLHLFLETLELHRARG
                     DLSSTRVSLAVTFSRVVAQNLRPQFFWFVLSYLPLAHFGPVPAAFAWAVLAHLSYDLE
                     RMFPAQFRALALELQSLHGVSAAWMVPPTPGEPTNALARTLATWQRIIDWQIREKNLI
                     RFHRQPVMEGAREAVLRLQPRQYRLAARAALRFTDAEGAEHFVRVKNGVEEGGVEVTV
                     VGDADVIARLPGTGELDANRDPLLPLLRSVLAINLRFHNPAEPFRRLFGLDGTGRETL
                     WGLPAPIEDTPVFHTAEPAEVDALMSAPTVVLKEKGTPATEILSEMIHRLVVDEKKVV
                     LLVGEKDLRDLRELLGAASKKNVPFLCVGAPPADLSVEERRRWLTHLVTDGAEGVTGP
                     IDLSRGLLVASGLINIGLDLPFRRVFFPKDRPDPRMFDAIVYVGLDRYPAVYAQVPIR
                     YAKDHARLIFSGAETDRPARVFRPKNREALLASGFTERDINDYEGNALDWVLRNSSAL
                     VARIVPKYADLFAPLEE"
BASE COUNT          231 a          531 c          511 g          304 t
ORIGIN      
        1 gttgacggtt gaaattccgg cgctgctcct ggggtcggtg gcgttgggcc cgttgggggc
       61 cctggcgggg ctggcggtct tcacgttcct gcatctcttt ttggagaccc tggaacttca
      121 tcgggcccgg ggggatttga gttcgacccg ggtgtccctg gcggtgacct tctcccgcgt
      181 tgtcgcccaa aatcttcgtc cgcaattttt ctggtttgtc ctttcctatc tgcccctggc
      241 ccattttggt ccggttcccg ccgccttcgc ctgggcggtt ctcgcccacc tctcctacga
      301 tttggaacgg atgttcccgg cccaattccg ggccctggcc ctggagcttc aatcgctcca
      361 cggcgtgtcc gccgcctgga tggtcccgcc caccccgggg gaacccacca acgccctggc
      421 ccggaccctg gccacctggc agaggatcat cgattggcag attcgtgaaa aaaatctgat
      481 ccggttccac cgccaacccg tgatggaagg cgcgcgggag gccgtcctcc gcctgcaacc
      541 gcgccaatac cgcctcgcgg cccgggccgc tcttcgtttc accgacgccg agggcgccga
      601 gcatttcgtc cgggtgaaga acggggtgga ggaaggcggc gtcgaggtaa cggtggtcgg
      661 cgacgccgac gtcatcgccc gccttccggg gacgggggag ctggacgcca accgcgatcc
      721 cctcttgccc ctgctgcggt ccgtgctggc catcaatttg cgttttcaca acccggccga
      781 acccttccgc cgccttttcg gcctggacgg caccgggcgg gaaacgctgt gggggctgcc
      841 cgcgcccatc gaggacaccc cggtgttcca caccgcggag cccgccgagg tggacgcgtt
      901 gatgtccgcc ccgacggtgg ttctgaagga aaaaggcacg ccggccacgg agattttgag
      961 cgaaatgatc catcggctgg tcgtggacga gaaaaaagtc gtcctcctcg tgggggagaa
     1021 ggaccttcgg gatctgcgcg aactcctggg ggcggccagc aagaaaaacg tccctttcct
     1081 ttgcgtgggc gcgcccccgg ccgatttgtc ggtggaggag cgccgccggt ggttgacgca
     1141 cctggtcacc gacggcgcgg agggcgtgac ggggcccatc gacttgagcc ggggcttgct
     1201 ggtggcgtcg ggcctgatca acatcgggct ggacctgccc ttccgccggg tctttttccc
     1261 caaggaccgg cccgatcccc ggatgtttga cgcgatcgtc tacgtgggcc tggatcgtta
     1321 ccccgccgtc tacgcccagg tgccgatccg ctacgccaag gaccacgccc ggctgatctt
     1381 ttcgggcgcg gaaaccgacc gtcccgcccg ggtgttccga cccaaaaacc gggaagccct
     1441 tttggcctcg ggctttaccg agcgggacat caacgattac gaaggcaacg ccctggactg
     1501 ggtgctgcgg aattcctcgg ccctggtggc ccggatcgtg cccaaatacg cggacctctt
     1561 cgccccgctg gaagagg
//
LOCUS       DAOQLS010000558         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_61057_length_1576_cov_3.461538, whole genome shotgun sequence.
ACCESSION   DAOQLS010000558 DAOQLS010000000
VERSION     DAOQLS010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61057_length_1576_cov_3.461538"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PK362_09435"
     CDS             complement(<1..393)
                     /locus_tag="PK362_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75089.1"
                     /translation="MSSKNGRTIFRRLLPGFLWGTLLQGLLVFGGLPLGAAPTDSLSF
                     TVSIVDADPPAAIADVSASSTVTPGQAVLAWTAPDEDAGGLNGEAVQSYVVKYATFSI
                     ASLGGNSALWWSLASPAPFPPVPQTPGNG"
     gene            complement(507..1415)
                     /locus_tag="PK362_09440"
     CDS             complement(507..1415)
                     /locus_tag="PK362_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75090.1"
                     /translation="MASGLKRRTRWSALAGLAVIAVPLWAKLSLGVRFPDIVLENVSP
                     GMVLNLRQAKGMPYVVINQSDFPVDVVVDVELPGPQHIDPKEGYEPAPKKEWVQVVPN
                     RFRLGAGDIGTAEVILSVPNDPALVGKHYQINLHARSLGTGQLALAVNHYIRFSVGSM
                     GPGALAKEKNRVAVGKLDLDMTPTLRLDKVPLGKSIALDALGGTLKITNRGDDPVKLK
                     FASNRVIASLREPGWTDPPHPEWLSMKPAVVKVKPNQVKPVKLFLNIPDAPENRGQKF
                     QFLITAELADLGIPLQYYTRVYVTTE"
BASE COUNT          267 a          494 c          500 g          315 t
ORIGIN      
        1 accgttcccg ggggtttggg gcaccggcgg gaagggggcg ggggaggcca ggctccacca
       61 aagggcggaa tttccgccca ggctcgcgat ggagaaggtg gcgtatttaa ccacgtagga
      121 ctgaacggct tcgccgttca gtcccccagc gtcctcgtcg ggcgcggtcc aggcgagcac
      181 cgcttgaccc ggggtcacgg tcgacgaggc actgacgtcg gcgatggccg cgggcgggtc
      241 ggcgtccacg atggacacgg tgaacgacag cgagtccgtg ggggccgctc ccagggggag
      301 gcctccaaag accaagagcc cctgcaacag ggttccccac aggaaccccg gcagaagccg
      361 ccggaagatc gttcgaccgt ttttcgagct caagagtcgc tcaactcctt atctttctat
      421 tcctctatta gggcgttccc ccggggggtt ggccttcgcc ccaccggttc gatcccccat
      481 gaaatgaaag gctcatcgaa atcccctcat tccgtggtca cgtagacccg ggtgtaatat
      541 tgaagcggga tccccaggtc ggccaattcc gccgtgatga ggaattggaa cttttgcccc
      601 cggttttccg gcgcgtccgg aatgttcagg aaaagcttca ccggtttgac ctggttgggc
      661 ttcaccttga ccaccgcggg tttcatggac agccattcgg gatggggtgg atcggtccat
      721 cccggttcgc gcaaactggc gatgacgcgg ttgctggcaa atttcagctt gaccggatcg
      781 tcgccgcggt tcgtgatttt gagcgtcccc cccaaggcgt ccagggcgat gcttttgccc
      841 agcgggacct tgtccagacg caacgtcggc gtcatgtcca ggtccaactt gcccaccgcc
      901 acgcggtttt tctccttggc cagggcgccg ggtcccatgg aaccgaccga gaaccggatg
      961 taatggttca cggccagggc caattgcccg gtgccgagac tgcgcgcgtg gaggttgatt
     1021 tggtagtgct tgccgaccag ggccgggtcg ttgggaacgg acaggatgac ttcggccgtt
     1081 ccgatgtcgc cggcccccaa tcggaaccga ttgggcacca cctgaaccca ctcttttttc
     1141 ggagcgggct cgtagccttc cttggggtcg atgtgttgcg ggcccgggag ctccacgtcc
     1201 accaccacgt ccacgggaaa gtcggactgg ttgatgacga cgtacggcat gcccttggcc
     1261 tggcggaggt tcaggaccat cccggggctc acgttttcca ggacgatgtc cgggaaacgg
     1321 acccccaacg acagcttggc ccacaggggg acggcgatca ccgccaaccc cgccagggcg
     1381 ctccaccgcg ttcgacgctt caaaccgctt gccatgtcgt tctccgtttt ttgtcgttgt
     1441 ccatcgtgtt gtcggtcaac ggaccgcaac gcgccgagac cttccggccg ttgccgccgt
     1501 cttttttatt gggcctccaa ttcgaccgtg aaggaccgct gggtgctgac gctcgaggag
     1561 gtcggcatgt ccaacc
//
LOCUS       DAOQLS010000559         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_61447_length_1571_cov_4.987467, whole genome shotgun sequence.
ACCESSION   DAOQLS010000559 DAOQLS010000000
VERSION     DAOQLS010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61447_length_1571_cov_4.987467"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            810..>1571
                     /locus_tag="PK362_09445"
     CDS             810..>1571
                     /locus_tag="PK362_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75091.1"
                     /translation="MTTNGPRTVLNLAWAVFGALHVISWHQKARPIPIASILPELRRE
                     PRQTPTDPTPFAFSYRGRPYNVKPLAAYDLAGLVVSHNDIGGLADIYHTKDSLDTKDV
                     CVIWGDNVATEDFHRVKFWNGPWTCYWRYPDGVRTAASQVSNNHLITQSDSIRQAIAR
                     VRVGDQIRVRGRLVAYQDAAHAEFWRTSSLTRGDTGNGACEVVLVENLEILKAGAPGW
                     WTLYLLSLGGLGALAALELVENLEILKAGAPGWWTL"
BASE COUNT          268 a          493 c          525 g          285 t
ORIGIN      
        1 acttaccttg aagtgcgcgc gccctacggg gtgaaaagct tttgacgtgc cgagccccgg
       61 agccgacggc ctccccaccg cgcggcaggc tgagcgtagc tgcggtggcg cagggtgccg
      121 acgcggcgga gcgacccgaa agacgaacca accggagtgc tcggcggggg tttttctccc
      181 caggcgacga atcaaccgga gcgggtttgg aacggattga ccgcagcggt cagcgtcctg
      241 gttgcggggg cgacggagcg agctggcccc gcgcgcgctt ttcgggcggg gccggcgagc
      301 gaccaaggcg cggcgcgaca gcgccggggt tttttcaacg ggggtttcgg gggcgagggc
      361 ggcggcccgg ccccgggcct gtgtccccct tggtccccgt ccctccattc cgcgggagaa
      421 atagagcggc cccgctgggc gcccggatag cggtcgacgg tcaaaaaaga ggagggaacg
      481 aaaaaatgac ggcaccccct ccccggccct cccccaaggg ggcgggggtc agaggtaagg
      541 cggccttgcg cgttggcacg ctccaggcat ttcctcgcct cccacggctc ggtcagagtc
      601 cgcgggtaac ctcaaaccct tctttgttgg tgggccgcct ccagccgctt cacgtggtat
      661 tccccggtca gggaatagtt ttcccaatag ctgtgggaca ggtttggaaa ggtcagcggg
      721 atgacattcg gcggcctttc atataccccg ccattttcct tccaaaaccc acgccccgcg
      781 gtgtgggttt tgaattttag ggggaccgat tgacgaccaa cggcccgcga acggtcttga
      841 accttgcctg ggcggtgttc ggcgccctcc acgttatttc ttggcatcaa aaagcgcggc
      901 ccatcccgat cgcctccatc ctcccggaac tccgtcgaga acctcgccaa acccccacgg
      961 acccgacgcc gttcgcgttc agttatcgtg ggcgccccta caacgtcaaa cccctggccg
     1021 cttacgattt agcggggctg gtggtcagtc acaacgacat cggcgggttg gccgatattt
     1081 accacaccaa agactccctg gacaccaagg acgtttgcgt gatttggggg gacaacgtgg
     1141 ccacggaaga ttttcaccgg gtgaagtttt ggaacgggcc ctggacctgc tattggcgtt
     1201 accccgacgg ggtccgaaca gcggcgtccc aggtgtccaa caaccacctg atcacccaaa
     1261 gcgattccat ccgccaagcc atcgcccggg tgcgggtcgg ggatcagatc cgtgttcgtg
     1321 gccggttggt ggcgtaccag gacgccgccc atgcggaatt ttggcgcact tccagcctga
     1381 cccgggggga cacgggcaac ggcgcctgcg aagtggtgtt ggtggaaaac ctcgagattc
     1441 tgaaggccgg cgcgcccggc tggtggacgt tgtatctgtt gtccctgggt gggctgggcg
     1501 ccctggccgc gctggagttg gtggaaaacc tcgagattct gaaggccggc gcgcccggct
     1561 ggtggacgtt g
//
LOCUS       DAOQLS010000560         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_61462_length_1571_cov_3.821900, whole genome shotgun sequence.
ACCESSION   DAOQLS010000560 DAOQLS010000000
VERSION     DAOQLS010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61462_length_1571_cov_3.821900"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PK362_09450"
     CDS             <1..508
                     /locus_tag="PK362_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC75092.1"
                     /translation="TNVHLGKSIEEIVGLFAPLFEKKKITGTVAVPAELPILALDEEK
                     MKQVVTNLISNAYKFTPEGGRITVSAEDTGETITVAVTDTGIGIPKDYVNQLFERFKQ
                     VPGTREKMGGPKGTGLGLAIAKGIVEAHGGKIWVESEVGQGSSFKFTLPKAATEASVQ
                     ANIFSRGG"
     gene            512..>1571
                     /locus_tag="PK362_09455"
     CDS             512..>1571
                     /locus_tag="PK362_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75093.1"
                     /translation="MAELENGPARKPRILVIDDEANLPIFLKDFLEESGFEVSMCYTG
                     KTAMRTALENPPDAIILDVDLPDIDGYSLCRSMRRTSTLRTVPIVMLTALADQRNEIA
                     GLKAGADDYLTKPIDTERLLARLNNAISRNIRELDANPLTHLPGNTSIIQEMERQLHQ
                     QNPFAVIYADLNNFKAFNDRYGFLRGDQAIKLAAQCLVLSVEKGASLSAGLVGSWFIG
                     HVGGDDFIVILPADQAEDTCKEIISKFDTQVPNLYDEEDRQRGFILGKNRQGQPLQYP
                     FIGMALVIVSNGDKRFTHPGEISSMASELKSFAKSFGKSAYVLDRRRNTADVLEGPVG
                     IVPPPAAAVGARRLPVMAS"
BASE COUNT          327 a          520 c          477 g          247 t
ORIGIN      
        1 gaccaacgtg cacctgggca agtccatcga ggaaatcgtg ggcctcttcg cgccgctctt
       61 tgagaaaaag aagatcaccg gaacggtcgc ggtgccggcg gaactcccca ttttggccct
      121 ggacgaggaa aagatgaagc aggtggtgac gaacctgatc tcgaacgcct acaagttcac
      181 gcccgagggc gggcgcatca ccgtttccgc cgaggacacg ggcgaaacga tcaccgtggc
      241 cgtgacggac acgggcatcg gcattcccaa ggactacgtc aaccagctgt tcgagcgctt
      301 caagcaggtg ccgggcacgc gcgagaagat gggcggcccc aagggcacgg gcctgggcct
      361 ggccatcgcc aagggcatcg tggaggccca cggcggaaaa atttgggtgg aaagcgaagt
      421 ggggcagggc agttccttca agttcaccct gcccaaggcc gcgacggagg cctcggttca
      481 agcgaacatt ttttcccggg ggggttaggc attggctgag ctggagaacg ggcccgctcg
      541 aaaaccgcgc atcctcgtca tcgacgacga ggccaacctg cctatttttc tcaaggattt
      601 cctggaggag agcggcttcg aagtgtccat gtgctacacg ggcaagacgg ccatgcgcac
      661 cgccctggaa aacccgcccg acgccatcat cctggacgtc gacttgccgg acatcgacgg
      721 ctactccctt tgccgcagca tgcgccgcac gagcacgctc cgcaccgtgc ccatcgtcat
      781 gttgaccgcc ctggcggacc agcgaaacga gatcgcggga ctcaaggccg gcgccgacga
      841 ttacctgacc aagcccatcg acaccgagcg cctcctggcc cggctcaaca acgccatcag
      901 ccggaacatc cgcgagttgg acgcgaaccc cctgacccac ctgccgggga acaccagcat
      961 cattcaggag atggagcgcc aactccacca gcagaacccc ttcgccgtca tctacgccga
     1021 cttgaacaat ttcaaggcct tcaacgaccg ttacggcttt ttgcgcggcg accaggccat
     1081 caaactggcg gcccagtgcc tggtcctgag cgtggaaaag ggcgcctccc tctccgccgg
     1141 gttggtgggg tcttggttca tcgggcacgt gggcggcgac gatttcatcg tcatcctgcc
     1201 ggcggaccag gccgaggaca cctgcaagga gatcatctcc aaattcgaca cccaggtgcc
     1261 gaacctctac gacgaggaag accgccagcg cggcttcatc ctgggcaaaa accgccaggg
     1321 ccagcccctg cagtatccct ttatcggcat ggcgctggtg atcgtctcca acggggacaa
     1381 gcgcttcacc caccccggcg aaatcagctc catggccagc gaattgaaga gcttcgccaa
     1441 atctttcgga aaaagcgcct acgtgctgga ccggcgccgc aacaccgccg acgtgttgga
     1501 aggccccgtg ggcatcgtgc cgccgcccgc cgcggccgtg ggcgcgagga ggctccccgt
     1561 gatggcgagc c
//
LOCUS       DAOQLS010000561         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_61507_length_1571_cov_2.353562, whole genome shotgun sequence.
ACCESSION   DAOQLS010000561 DAOQLS010000000
VERSION     DAOQLS010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61507_length_1571_cov_2.353562"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /gene="ribF"
                     /locus_tag="PK362_09460"
     CDS             <1..914
                     /gene="ribF"
                     /locus_tag="PK362_09460"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNC75094.1"
                     /translation="LMGRPVVLTLGTFDGVHRGHQALLARARRRARVLGGEVLAVAFD
                     RPPRLFFHPEPPPALITTPEERAVLLRRFGADRVDRLAFGRALARLTAADFLRKYVRG
                     RWRARECVVGFNFAFGRGREGDFPTLQRLARRWNLRVHAVGPVKHRGRTVSSGLIRRC
                     LRAGQTGEAADLLGHAVALSGRVVRGRGLGRRLGFPTANLRVSPDKILPSGVFSVGVT
                     LPSGTRRGGMMNIGRRPTLGTRGGRTVEVHLFDFLGDLTGQSLSVECLAPLRAEKKFP
                     SLAALVRQLRLDERRARTRVARAGWLA"
     gene            942..>1571
                     /locus_tag="PK362_09465"
     CDS             942..>1571
                     /locus_tag="PK362_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNC75095.1"
                     /translation="MENPARRSPGPLVFWSLVALVFLWSGHRPFDRITWVLEVAPAVL
                     GAGILWATRRRFPFTPTVLFLIGLHMVILMIGGHYTYARVPLFDGIRDVFGLARNHYD
                     RLGHFAQGFVPALIAREILIRRSPLKPGAWLFFLVCCVALSLSAVYELVEWRVSVATG
                     SAGDAFLGTQGDPWDTQWDMALALMGAVTSQIALGRRLFFDPGFSANGAV"
BASE COUNT          188 a          535 c          523 g          325 t
ORIGIN      
        1 gactgatggg tcggccggtc gtcctgaccc tcggcacttt cgacggcgtt caccgggggc
       61 accaggccct tctggcccgg gcccgccgcc gggcccgggt tctcggcggc gaggtgctcg
      121 ccgtggcttt tgatcgtccg ccgagacttt tttttcatcc cgaaccgccc cccgccctga
      181 tcacgacccc ggaggaacgc gccgttctgc tccgccgctt cggcgcggac cgggtggacc
      241 ggctggcctt cggccgggcc ctggcgcgct tgaccgcggc ggatttttta aggaaatacg
      301 tccggggccg ctggcgcgcc cgggaatgcg tggtcggatt taatttcgcc ttcggacgcg
      361 gccgggaggg ggatttcccg accctccaac gcctcgcccg gcgatggaac ctccgggtcc
      421 acgcggtggg gcccgtgaag caccgggggc ggaccgtgtc ctcggggctc attcggcggt
      481 gtctccgcgc gggccaaacg ggggaggcgg ccgatcttct cggccacgcc gtcgcccttt
      541 cgggacgggt cgttcgagga cggggcctgg gccgtcggtt gggctttccc accgccaacc
      601 tgcgggtttc cccggataaa attttgccgt ccggcgtgtt ttcggtcggc gtgaccctgc
      661 cctccggaac ccggcggggg gggatgatga acatcggccg ccgcccgacc cttgggaccc
      721 ggggcggccg cacggtcgaa gtccatttgt tcgattttct cggcgatctg acgggccagt
      781 ccctgtcggt cgagtgcctc gcgccgcttc gggcggaaaa aaagtttccg tccctcgcgg
      841 cgctcgtccg gcaactgcgg ttggacgaaa ggcgggcccg gacccgcgtg gcccgcgcgg
      901 gatggctggc gtaaacacgg gtttgggcct atcctaaacc catggaaaat ccggcgcgtc
      961 gctccccggg acccctcgtt ttctggtcgt tggtcgccct cgtttttctc tggtcgggcc
     1021 atcgaccctt cgaccggatc acctgggtct tggaggtggc cccggccgtt ctcggagcgg
     1081 gaattctctg ggcgacccgc cgtcgattcc ccttcacccc caccgtcctt tttctgatcg
     1141 gcctccatat ggtgattttg atgatcggcg gccattacac ctacgcccgg gttccgttgt
     1201 ttgacgggat tcgggacgtt ttcggcctcg cgcgcaacca ttacgaccgc ctgggtcact
     1261 tcgcccaggg gtttgtcccg gcgctcatcg cccgggaaat cctgattcgc cgctctccgc
     1321 tcaaacccgg ggcctggctc tttttcctgg tgtgttgcgt ggccctgtcc ctcagcgccg
     1381 tttacgaact cgtcgaatgg cgggtgtccg tggccaccgg aagcgccggc gacgcttttt
     1441 tgggaaccca gggcgatccc tgggacaccc aatgggacat ggcgctggcc ttgatgggag
     1501 cggtcacatc ccagatcgcg ctgggccgcc ggctcttctt tgatccaggg ttcagcgcca
     1561 acggcgcggt c
//
LOCUS       DAOQLS010000562         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_62495_length_1558_cov_3.357951, whole genome shotgun sequence.
ACCESSION   DAOQLS010000562 DAOQLS010000000
VERSION     DAOQLS010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62495_length_1558_cov_3.357951"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /gene="xseA"
                     /locus_tag="PK362_09470"
     CDS             complement(<1..1252)
                     /gene="xseA"
                     /locus_tag="PK362_09470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNC75096.1"
                     /translation="MAPHPRAASSINETTDDGLSVSQVNQSIAKTLEDRFSEAFWVKG
                     ELQGYDRDAAKAGQRRWGQVYFELIEKEPGADTVRASIKAVLWGDAQNTLRRKLAEVS
                     GDLKLQDGLSVKVLCTVDFYWPRASLQLKVVDVDPHFTLGDMERARRELLEKLKKSGR
                     LEKNRSLPFPELPLGVGLITSDGSAAYHDFVQELKSSGYAFTVYFVDAHTQGAETERE
                     VSRALRQLGARPEVQVVALIRGGGSRSDLIWFDKEKIAEAVADCPVPVVTGIGHEIDS
                     SVADIVAHTARKTPTAVAQFLVERVDAVDRLLADHAGALVRGTRALLDRLGRDLSSAA
                     GAWREAAGAALARSQGALREQRGLLAAALKRQLGMAGERLRTAPDRLDRSVRAVLVRA
                     SDRLAAAGRECALKDPRRLLERGYS"
     gene            complement(1219..>1558)
                     /locus_tag="PK362_09475"
     CDS             complement(1219..>1558)
                     /locus_tag="PK362_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75097.1"
                     /translation="IPLPMGKSKEIKKTVTTGDLTVLADFFTSTERFRVDAGHFGFSG
                     LGESKTWNGLENLKRFVKIILERRPDVPLNRGARWVMENKTLATAGYTDRGQFEKECR
                     WLLTLARLPR"
BASE COUNT          270 a          519 c          484 g          285 t
ORIGIN      
        1 aactgtagcc ccgttccaga agccgccggg ggtccttgag cgcgcattcc cgcccggccg
       61 cggccaaacg gtccgacgcc cgaaccagga cggcgcggac gctccgatcc aggcggtccg
      121 gggccgtccg taggcgttcc ccggccattc ccaattgacg tttgagcgcc gccgccagaa
      181 ggccccgctg ttcccgaagg gccccctggg aacgggccaa agcggccccg gccgcttccc
      241 gccaggcccc cgccgccgag gacaaatccc gccccagccg gtccaagagg gcccgggttc
      301 cccggacgag ggctccggcg tggtcggcca acaggcggtc gacggcgtcc acccgttcca
      361 ccaaaaattg cgccaccgcc gtgggcgttt tgcgcgccgt gtgggccacg atgtccgcca
      421 cggaggaatc gatctcgtgg ccgattcccg tgaccaccgg cacggggcag tccgccacgg
      481 cttcggcgat cttttcctta tcgaaccaga tcaggtcgct ccgggaaccc ccgccccgga
      541 tcagggccac aacctggacc tcgggacggg cgcccagctg tcggagggcc cgggacacct
      601 cccgttccgt ctcggcgccc tgggtgtggg cgtccacgaa ataaaccgtg aaggcgtagc
      661 cggagctttt cagttcctga acgaaatcgt gataggcggc gctcccgtcg gaggtgatga
      721 gaccgacgcc gaggggcagt tccggaaagg gcagggaccg gttcttttcc aaacggccgg
      781 atttctttaa tttttccaac aactcccggc gggcccgctc catgtccccc agggtgaagt
      841 gggggtccac gtccaccacc tttagctgaa gggaggcccg gggccaataa aaatccaccg
      901 tgcacaggac tttgaccgac aacccgtcct gaagttttaa atccccggac acctcggcca
      961 gcttccgtcg cagggtgttc tgggcatcgc cccacagaac ggccttgatg ctcgcgcgca
     1021 ccgtgtcggc cccgggttcc ttctcgatca attcgaaata aacctgcccc cagcgccgct
     1081 ggccggcttt ggcggcgtcc cggtcgtagc cctggagttc gcctttgacc cagaaagcct
     1141 cggaaaagcg gtcctccagg gttttggcga tggattggtt gacctggctg acggagagcc
     1201 cgtcgtcggt cgtttcgttt atcgaggaag ccgcgcgagg gtgaggagcc accggcattc
     1261 cttttcaaac tggccccggt cggtatagcc cgccgtcgcg agggttttgt tctccatcac
     1321 ccaacgggcg ccgcggttga gcggcacgtc gggccgccgc tcgagaatga ttttgacgaa
     1381 gcgtttcaaa ttctccaatc cgttccaggt tttggattcg cccaagccgg aaaacccaaa
     1441 atgtcccgcg tcgacgcgga aacgttcggt ggaagtgaaa aaatccgcca agacggtgag
     1501 gtctccggtg gtgacggtct ttttgatctc tttggatttt cccatgggca gggggatt
//
LOCUS       DAOQLS010000563         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_62637_length_1556_cov_4.469687, whole genome shotgun sequence.
ACCESSION   DAOQLS010000563 DAOQLS010000000
VERSION     DAOQLS010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62637_length_1556_cov_4.469687"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1139)
                     /locus_tag="PK362_09480"
     CDS             complement(171..1139)
                     /locus_tag="PK362_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75098.1"
                     /translation="MTPAGSATILIADDDRYAQKALETLLTIEGFQVVTAQDGQEALE
                     RIQERPPDLCIIDFDMPRLNGMETCRRIKNHHTTRLLPVIMVTGLLPDEERVRAIEAG
                     CDDFLPKPYQPAELMTRIRSLLRIKSLTDELEDAEAILMTLVRTIEAKDRYTLGHADR
                     VGRYAVTLGRALGCNAFELDSLRKAGMLHDIGKIGIPDAILQKAGPLDDAEWEVMRKH
                     PTIGCEICQRLKSLEGILPMIRHHHECLDGTGYPDGLKGAQVPKLVRIVNVVDIYDAL
                     TSRRSYKDAYPVQKAFRTMWEEVERGWWDGDVLKVWEELVKRESLG"
     gene            complement(1242..>1556)
                     /locus_tag="PK362_09485"
     CDS             complement(1242..>1556)
                     /locus_tag="PK362_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75099.1"
                     /translation="LKDAGWKPAFESTVFRPGDLLLRSEVIMPRWWFRPRSMRPLVRY
                     EYSSRFPLRVMDNLGAAGFYASAWGALPYTLSREPLDRYTLFEVVSATGPTEAPPSRP
                     GA"
BASE COUNT          274 a          482 c          529 g          271 t
ORIGIN      
        1 gccagcacgg ccttggccgc gcccaggccc gaccggccca cgacccggac gccccggcgg
       61 gccagccagg ccttcagttc gggcaaggcc cgccgcgccg cggttttatc ggggttgtgg
      121 aaaagggcga tgggcgaaaa ggaaccgggg gatttcgtca tgccgaactc tcagccgagg
      181 ctttcccgtt taaccaattc ctcccacacc ttgagaacgt cgccgtccca ccagccccgc
      241 tccacctcct cccacatggt gcggaaggct ttctgcaccg ggtaggcgtc tttgtaactc
      301 cgccgggaag tgagggcgtc gtagatgtcg accacgttga cgatgcggac cagcttgggc
      361 acctgggcgc ccttaagccc gtccgggtac ccggtgccgt ccaggcattc gtggtggtga
      421 cggatcatgg gcaggatgcc ttccaaactt ttgaggcgct ggcagatttc gcacccgatg
      481 gtcgggtgtt tgcgcatgac ctcccactcg gcgtcgtcca ggggaccggc cttttgaagg
      541 atcgcgtcgg ggatgccgat ctttccgatg tcgtggagca tgcccgcctt gcggagggaa
      601 tccagctcga aggcgttgca gcccagggcg cggcccaggg tcacggcgta gcggcccacc
      661 cggtcggcgt ggcccagcgt gtagcggtcc ttggcttcga tggtccgcac gagggtcatc
      721 aaaatcgcct cggcgtcctc caactcgtcc gtcagggact tgatgcgcag gagggaccgg
      781 atgcgggtca tgagctccgc gggctggtag ggcttgggaa gaaaatcgtc gcacccggct
      841 tcgatggcgc gcacacgctc ttcgtccggc aacagccccg tgaccatgat gacgggaagg
      901 aggcgcgtgg tgtggtggtt tttgattcga cgacaggttt ccatgccgtt caggcggggc
      961 atgtcaaaat cgatgatgca gaggtcgggg gggcgctcct ggatccgctc cagggcctcc
     1021 tgaccgtcct gggcggtcac gacctggaac ccttcgatcg tcaacagtgt ttcgagggct
     1081 ttttgggcgt agcggtcgtc atcggcaata agaatcgtgg ccgaacccgc gggcgtcata
     1141 ggaggagatg ataccagatt ccccccggcg gctcaacgga aaaggccccg ggaggccgac
     1201 ggtttacccc ggcgaaatca aggcgaaagc gcctttcgcg ttcaagcccc gggccgggac
     1261 ggcggcgctt cggtcggacc ggtcgccgag acgacttcaa agagggtgta tcggtccaaa
     1321 ggctcccggg acagggtgta aggcagggcc ccccaggccg aggcgtaaaa tccggcggcg
     1381 cccaggttgt ccatgacccg caggggaaat cgggaggaat actcgtaccg gaccagggga
     1441 cgcatgctcc gggggcggaa ccaccagcgc ggcatgatga cctccgatcg caggagcaga
     1501 tcccccgggc ggaaaaccgt ggattcaaag gccggtttcc acccggcgtc cttcag
//
LOCUS       DAOQLS010000564         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_62645_length_1556_cov_4.008661, whole genome shotgun sequence.
ACCESSION   DAOQLS010000564 DAOQLS010000000
VERSION     DAOQLS010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62645_length_1556_cov_4.008661"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1556
                     /locus_tag="PK362_09490"
     CDS             <1..>1556
                     /locus_tag="PK362_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75100.1"
                     /translation="KDVDGRPRTFTDNEIHQILSGWLMLMGQSGLVNRESFLHAREEL
                     RQLLAKEVVTRDQEAVDSHAAFAPYMVARLQETGQIPIFLARDALSIDEFRRYVDRLN
                     DQTSAAAVVYHPGIFTPVGPNRRSDDKELSQIIKSIKNIMDESFREIVAEGLFPYDQL
                     GHKSAAVRERIEEKFKEKTAALIEGDALFADYANQIYRQFRSHGLPMTRSFVVVDTYG
                     SGKSALFVKSVIEYFAAKERGREATPFKVDVLLGWSNHVSLSQPELSDFYAVDTRDKA
                     FLDLYWPFQYAAIHNAKSHFPMFGVKTNPTKLLSLGYQSLALYNQAVRFVRGEVPIQK
                     AGPTTDVRRDRGPPATLPGLVPLIARLYRLNPNSPEDLNTIVTEKAPLVESALFGLFY
                     FGAMMLMSSLDVLAAGYFGVPLSLSPTVFNQYSLFVLANLAFGALHARVYTYQDGQWR
                     IEQNPDGSPKTASWATRLSLAVRGMGIHSPLLLTSLTPLLGPLVTILGAAATVVLHMV
                     HNQLAAQFGW"
BASE COUNT          296 a          531 c          419 g          310 t
ORIGIN      
        1 caaagacgtc gacggccgcc cccggacttt taccgataac gaaattcacc aaattctttc
       61 cgggtggctg atgttgatgg ggcaatcggg cctggtcaac cgggaatcct tcctgcacgc
      121 ccgggaggaa ttgcggcagc tgttggccaa ggaggtcgtc acccgggacc aggaagcggt
      181 ggacagccac gcggctttcg ccccctacat ggtcgcccgc cttcaggaaa ccggccagat
      241 tccgatcttt ttggcccggg acgccttgtc catcgacgaa ttccggcggt acgtggaccg
      301 gctgaacgac cagacctccg ccgccgccgt ggtttaccat cccggcattt tcacgccggt
      361 gggccccaac cgccgcagcg acgacaagga actctctcaa atcatcaaat ccatcaagaa
      421 catcatggac gagagtttca gggagattgt cgccgaaggc cttttcccct acgatcagtt
      481 gggtcacaaa tcggcggcgg tccgggagcg catcgaagaa aaattcaaag agaaaacggc
      541 ggccctcatc gaaggcgacg cgctcttcgc ggactacgcc aatcagatct atcgccaatt
      601 tcgctcccac ggattgccga tgacgcggtc ctttgtcgtg gtcgacacct acgggtcggg
      661 caaatcggcg ttgttcgtca aatccgtcat cgaatatttc gccgccaagg aacggggtcg
      721 ggaagccacg ccctttaaag tcgacgtcct cctggggtgg tccaaccacg tttccctctc
      781 ccagccggag ctatcggatt tttacgcggt ggacacccgg gacaaggcgt tcctggatct
      841 ttactggccg ttccagtacg ccgcgattca caacgcgaaa tcccatttcc cgatgttcgg
      901 cgtaaagacc aacccgacga agttgctctc cctgggatac cagtcgctgg ccctttacaa
      961 ccaggccgtc cgtttcgttc gcggcgaggt tcccattcaa aaagcgggcc cgacgaccga
     1021 tgtccggcgg gaccgcggcc cgccggcgac ccttcccggc ctcgttccgc tcatcgcccg
     1081 gctctaccgc ctgaacccca acagccccga ggacctcaac acgatcgtca cggaaaaggc
     1141 gccgttggtg gaatcggcgc tcttcggact cttctatttc ggcgcgatga tgctgatgtc
     1201 ctccctcgac gtccttgcgg ccggctattt cggagttccc ctctctctca gcccaaccgt
     1261 cttcaaccaa tattccctct tcgtgctggc caacctcgcc ttcggcgcat tgcacgcccg
     1321 tgtctacacg tatcaagacg gccaatggcg gatcgaacaa aatccggacg ggtccccgaa
     1381 aacggcctcc tgggccacgc gcctgagcct ggccgtgcgc ggcatgggca ttcactcgcc
     1441 gcttcttttg acaagcttga ccccgcttct gggcccgctg gtgaccatcc tcggcgccgc
     1501 cgccaccgtc gtcctccaca tggtccacaa ccagctggcc gcccaattcg gctggc
//
LOCUS       DAOQLS010000565         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_62799_length_1554_cov_5.188793, whole genome shotgun sequence.
ACCESSION   DAOQLS010000565 DAOQLS010000000
VERSION     DAOQLS010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62799_length_1554_cov_5.188793"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PK362_09495"
     CDS             <1..337
                     /locus_tag="PK362_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75101.1"
                     /translation="GKSAGGGATESPAAASKDPAATKPSKEAAAAKSDKQKEQEKKLA
                     EQQRQIQEQMNRSLQDSIRQNQMILQQLQNERANQQAIQNMIQTQRQTQQAIDAARQA
                     SQASQQQKR"
     gene            359..895
                     /locus_tag="PK362_09500"
     CDS             359..895
                     /locus_tag="PK362_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75102.1"
                     /translation="MNENVSIADSQRLVKLVGYFATPFALMVGGLGVIVSGATDTVRA
                     VCVGLLGFSGFLNLIFPKFLEGQSADKKGPNVRVRMALNVLVNALLVYFLGVDFPSIW
                     LILALSPFATAIYGSRRKTAVAAATSVAILLTVHVLRGDHRFVVWAETLARVGFIVLI
                     SLMINGVANLVSRPEKIL"
     gene            964..>1554
                     /locus_tag="PK362_09505"
     CDS             964..>1554
                     /locus_tag="PK362_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC75103.1"
                     /translation="MADVHGETPLSGVYVVGDLKGVPLLKFAADSGARAIRRIAAERR
                     GAPLLAPEDGVEVLILGAGVAGAAAALEARKTGLSFAVIEAAEPFFTVANFPQGKPIF
                     AYPTDLVPAGELQFSATVKESLLEDLRSRWASSGISTVAGRAERLRRRGAGWEVLLAD
                     GSARRAARVVIALGRSGHFRRLGVPGEDRDKVTHRLH"
BASE COUNT          286 a          499 c          509 g          260 t
ORIGIN      
        1 gggcaagtcg gcgggcggcg gggcaacgga atcgcccgcg gccgcgtcca aggacccggc
       61 ggcgaccaaa ccgtcgaaag aagcggcggc ggcgaaatcc gacaagcaaa aagagcagga
      121 aaagaaattg gcggaacagc aacgacagat ccaggagcag atgaaccgca gccttcagga
      181 ttccatccgc caaaaccaaa tgattcttca acagctgcaa aacgagcggg cgaaccaaca
      241 ggccattcaa aacatgattc agactcaacg gcaaacgcaa caggccatcg acgcggcccg
      301 ccaagcgagc caggcgtccc aacaacaaaa acgctgacaa gcggacaagg ggaacaccat
      361 gaacgaaaac gtgagcatcg ccgacagtca acgtctggtt aaactcgtgg ggtatttcgc
      421 gacgcccttc gccctgatgg tgggaggcct gggggtgatc gtgtcgggag ccaccgacac
      481 ggtgcgcgcc gtgtgcgtgg gtctcctggg gttcagcgga ttcctgaacc tgatatttcc
      541 caaattcctc gaggggcaat cggccgacaa aaagggcccg aacgtgcgcg ttcggatggc
      601 cttgaacgtt ctcgtcaacg cgctcctggt ctacttcctc ggcgtggatt tcccgtccat
      661 ctggttgatt ctggccctgt cgcccttcgc cacggccatt tacgggtccc gccgcaaaac
      721 ggcggtggcg gccgcgacgt ccgtggcgat tctcctcacc gtgcatgtcc ttcgggggga
      781 ccaccggttc gtcgtttggg cggagaccct ggcgcgggtg gggttcatcg ttctcatcag
      841 cctgatgatc aacggcgtgg ccaacctggt gtcccgcccc gaaaaaattc tctgaacgtc
      901 gtcgcccgct actatcgctg gctccacggc cgctggccgg cggggcgggt ggagccgttg
      961 cccgtggcgg acgtccacgg ggagaccccg ctgtccgggg tgtacgtcgt gggcgatttg
     1021 aagggcgtcc ccctcttgaa atttgcggcg gattccggcg cccgggcgat tcgacgcatc
     1081 gcggcggaac gccgaggcgc cccgctcctc gcgccggagg acggcgtcga ggtcctgatc
     1141 ctcggggccg gcgtcgcggg cgccgccgcg gccttggaag cccggaaaac cgggctgtcc
     1201 ttcgccgtga tcgaagccgc cgaacctttc ttcaccgtcg ccaacttccc ccaaggcaaa
     1261 cccatcttcg cctatccgac cgacctggtc cccgcgggcg aactgcaatt ttccgccacg
     1321 gtcaaggaat cccttttgga ggacctgcgg tcccgttggg cctcctccgg catttcgacg
     1381 gtggccggcc gggcggagcg attgcggcgc cgaggggcgg gatgggaagt cctcctggcc
     1441 gacggatccg cgcggcgggc cgcccgggtt gtcatcgcgc tggggcggag cggtcatttt
     1501 cgtcgattgg gcgtgcccgg ggaggaccgg gacaaggtca cccatcgtct gcac
//
LOCUS       DAOQLS010000566         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_62951_length_1552_cov_3.953240, whole genome shotgun sequence.
ACCESSION   DAOQLS010000566 DAOQLS010000000
VERSION     DAOQLS010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62951_length_1552_cov_3.953240"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PK362_09510"
     CDS             <1..973
                     /locus_tag="PK362_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNC75104.1"
                     /translation="DLLNRVASIRRRCARELGFVLPPVRIRDSSALYNNTYVIKIKGA
                     EVARGDLMPGYCLAMGGDGIKNVRGLPAKEPTFGLPALWVAETDRRSAEMEGLTVVDN
                     ASVVATHLTEVLRDHAHEILGRQEVQTLLNDLKGRGQTALLEELIPGVLSAGAVHRVL
                     QNLLSEKISIRNLLQILEALSEAAGVTKDPDLLTEYARASLARQISQQYRDEEGTLYA
                     ATLAPDLERRLVESMPKGERTARLALDPALSDDVARRLLELFSAPVGAADRPVLLCSA
                     SLRAAVRRHFERFVPRLTVLSYNEITADTRVKSIGVVGAETPQPVAS"
     gene            990..>1552
                     /locus_tag="PK362_09515"
     CDS             990..>1552
                     /locus_tag="PK362_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC75105.1"
                     /translation="MRNATSEKENAGRAPATPAALENAWRRFLSDRDASARETILAQF
                     APLVRHVVGRMPVALVPQVDRDDLESAGAIGLLKALDRFDPSRNVKFDTFAYRWIQGS
                     VLDHLRRLDPLSRGGRRRVERYKDVKNEMRERLGRDPSDLEIAAELGLTPAEMRGLHQ
                     EVIHDGEMSLDGGPRGSEEGDGAGPDLL"
BASE COUNT          273 a          480 c          542 g          257 t
ORIGIN      
        1 ggatttgttg aatcgggtgg cctcgatccg acggcgctgc gcccgggaac tggggttcgt
       61 cctccccccc gtgcgcattc gggattccag cgccctctac aacaacacct acgtcatcaa
      121 aatcaaaggc gccgaagtgg cccgggggga tctcatgccc ggctactgcc tggccatggg
      181 gggcgacggc atcaagaacg tccggggatt gcccgccaag gagcccacgt tcggcttgcc
      241 cgcgctctgg gtggcggaaa cggaccggcg ttccgccgaa atggagggac tcacggtggt
      301 ggacaacgcg tcggtggtgg ccacccatct caccgaggtc ctgcgggacc acgcccacga
      361 aattttgggc cgacaggaag tccaaaccct gttgaacgac ttgaagggcc ggggccaaac
      421 ggccctcctg gaggaattga tcccgggggt tctatcggcc ggggccgtcc accgcgttct
      481 gcaaaacctt ttgagcgaaa aaatatcgat ccgcaatctg ctgcagatcc tcgaggcgtt
      541 gagcgaagcc gcgggggtga caaaagatcc cgacctgtta acggagtacg cccgggcctc
      601 tctggcgcga caaatttccc agcagtaccg ggacgaggaa ggaacgttgt atgccgccac
      661 cctggccccg gacctggagc ggcggctcgt ggaatcgatg cccaaggggg agcggaccgc
      721 ccgcctggcg ctggatcccg ccctttcgga cgacgtggcc cggcggctgt tggaactctt
      781 cagcgcgccg gtcggagcgg cggaccgtcc ggtgctcctt tgttcggcgt ccctgcgggc
      841 ggcggtccga cgacattttg agcggtttgt gccgcggttg acggtgctct cctacaacga
      901 gatcaccgcc gacacgcgcg tgaaatccat cggcgtcgtg ggggccgaaa cgccccagcc
      961 ggtggcgtct tgaattcggg aggatcctga tgaggaacgc aacgtccgag aaggaaaacg
     1021 cgggacgagc ccccgccacg cccgcggcgc tggagaacgc ctggcggcgg tttttatccg
     1081 atcgcgacgc ctcggcgcgg gaaacgattc tggcccaatt cgcgccgttg gtgcggcacg
     1141 tggtgggacg gatgcccgtg gccctggtgc cccaggtcga ccgggacgat ttggaaagcg
     1201 cgggcgccat cggtttgctg aaggccttgg accggttcga tccgagccgc aacgtcaaat
     1261 tcgacacttt cgcctaccgg tggatccagg gcagcgtgtt ggatcatttg cggcggctgg
     1321 atccgttgag ccgggggggg cgccggcggg tggagcgcta caaggacgtc aaaaacgaaa
     1381 tgcgggaacg cctgggtcgg gacccctcgg atctggaaat cgccgcggag ctcggtttga
     1441 cccccgcgga gatgcggggt ctccaccagg aggtcattca cgacggcgaa atgtccctgg
     1501 acggcgggcc ccggggctcg gaggagggcg acggagcggg cccggacctc ct
//
LOCUS       DAOQLS010000567         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_63026_length_1551_cov_3.820856, whole genome shotgun sequence.
ACCESSION   DAOQLS010000567 DAOQLS010000000
VERSION     DAOQLS010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63026_length_1551_cov_3.820856"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /gene="dnaG"
                     /locus_tag="PK362_09520"
     CDS             complement(<1..847)
                     /gene="dnaG"
                     /locus_tag="PK362_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNC75106.1"
                     /translation="MGVSRDTLDRIKSRLDTLEIIREAVPSLKQSGPRWKGNCPFHNE
                     KTPSFFYMPDKGLWHCFGACNEGGDVFRFVMKTQNMPFPEALRELARRAGVPLEWDRG
                     DDASSRRAKERDELLGLLEESAKFYADALKTQADAEPARRHLAERGIRRETIDAFALG
                     FAPRRDSFLDRALKKGTAIEPLLKVGLAARSDRTGRYHDPLGGRLIFPIRDPYGQVVA
                     FGGRTLEEEGGPKYLNSPESPVYTKGRQLYGLYEGRTTLREKAQAIVVEGYMDVVALH
                     QAGFAT"
     gene            complement(959..1159)
                     /gene="rpsU"
                     /locus_tag="PK362_09525"
     CDS             complement(959..1159)
                     /gene="rpsU"
                     /locus_tag="PK362_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNC75107.1"
                     /translation="MVFVKVRDGESIEEALRRFKRECERNGIMQEIKRREHYEPPSVK
                     RKRKQAEARRKMRRRMMRQNRG"
     gene            complement(1385..>1551)
                     /locus_tag="PK362_09530"
     CDS             complement(1385..>1551)
                     /locus_tag="PK362_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNC75108.1"
                     /translation="GGAALRAAQQLAAKFSVVEKGFRLVVNNGSDAGQAVDHLHYHFL
                     AGRRLGWPPG"
BASE COUNT          274 a          502 c          489 g          286 t
ORIGIN      
        1 ccgtggcgaa ccccgcctgg tgaagcgcca ccacgtccat gtagccttcc acgacgatgg
       61 cctgggcctt ctcccgcaac gtcgtacggc cttcgtacag cccgtagagc tggcgaccct
      121 tcgtgtagac cggggattcc ggcgagttca aatacttggg gccgccctct tcctccagcg
      181 tccggccgcc gaaggccacc acctgaccat acggatcacg gatcggaaag atcagtcgcc
      241 cgcccagggg atcgtggtag cgccccgtcc ggtcggaccg ggccgccaac cccaccttca
      301 ggagcggttc gatggcggtg ccctttttca aggcccggtc cagaaaactg tcccgccggg
      361 gggcgaaccc cagggcgaaa gcgtcgatgg tctcccggcg gatgccccgc tcggccagat
      421 ggcgccgggc cggttccgcg tcggcctggg tcttcaaggc gtcggcgtag aacttggccg
      481 attcttccaa caaccccagc agttcgtccc gctccttggc gcggcgggag gaggcgtcgt
      541 cgccccggtc ccattccagg gggacaccgg cgcggcgcgc cagttcccgc agggcttcgg
      601 ggaagggcat gttttgggtt ttcatcacga agcggaagac gtcgccgcct tcgttgcagg
      661 cgccgaagca gtgccacagg cccttgtccg gcatgtaaaa gaacgacggg gttttctcgt
      721 tgtggaaggg gcagttgccc ttccaacgag gccccgattg tttcaggctc gggaccgcct
      781 cccggatgat ttcgagggtg tccagccggg atttgatgcg gtccaacgtg tcgcgggaaa
      841 cgcccatgtc atgcgccctc ggccgacgat ccggccggcg gccccgggag acgcccccgg
      901 gacgcgcggc tcggcgtcgc gatgtgtgga gaaggaaacg aacggccgcc cggaggggct
      961 agccgcggtt ttgacgcatc atgcgtcggc gcattttccg ccgcgcttcg gcctgtttcc
     1021 gtttgcgttt cacggaaggg ggctcgtagt gttcgcggcg cttgatttcc tgcatgatgc
     1081 cgttgcgctc gcattcgcgt ttgaaacgac gaagagcttc ttcgatggac tccccgtctc
     1141 gaactttgac aaaaaccatg gacgatgaca cccccttccc gttatggatg gaattcgatg
     1201 gaaataaaaa gcccctgcac cggcgctgga tcggccggcc aaggtatacc cacgttaaca
     1261 acgtcgcggg gttttggttc cgccaaggaa gattccttaa acggaatctc cacttgcccg
     1321 aacttcggac aagtgcggaa acccattaaa cccggctgcg ccgggccaaa aattgaagtt
     1381 tccgttaacc cggcggccag ccgagtcgac gacccgctaa aaagtgatag tgcagatggt
     1441 ccacggcctg gcccgcgtcg gaaccgttgt tgaccaccag gcgaaaaccc ttttccacga
     1501 cggaaaactt ggcggccagc tgttgggcgg cgcggagcgc ggcccccccg a
//
LOCUS       DAOQLS010000568         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_63128_length_1550_cov_2.167224, whole genome shotgun sequence.
ACCESSION   DAOQLS010000568 DAOQLS010000000
VERSION     DAOQLS010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63128_length_1550_cov_2.167224"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..729
                     /locus_tag="PK362_09535"
     CDS             163..729
                     /locus_tag="PK362_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75109.1"
                     /translation="MKIMLLVLCLILSLQLAAEEKPNPAHNGHADELIGQEKETHADN
                     DDVGEESHADGDEHKEEGHEEAEEASSNVGPGNAVTAADPERGLQLSPEAFQTLEIRS
                     VSVPAKGVLPKSAIVTFKDEVGVYRLRDGWFKLVEGSAKNRNSDVVFIPHRSSDLRSG
                     DSVVVHGAPLLRVAELDAFSTGEAGHGH"
     gene            748..>1550
                     /locus_tag="PK362_09540"
     CDS             748..>1550
                     /locus_tag="PK362_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC75110.1"
                     /translation="MIEKLVRFSVNNRGLVFFLALLLAGLGWVSFQALPIDAVPDITN
                     NQVQVNTAVEGLTPEEIERYITVPIENGMGGLADLIQTRSISRIGLSQVTLVFEDRVD
                     IYRARQVVAERLQGVIPQLPEGILPGLGPVTTGLGEVYFYTIQASTPAANPTERVAQL
                     MELKSLQDWYLKPRLLTVPGVAEVNSIGGFEKQFHVQPNVRMMARYGLHFSDLIDALE
                     RTNRNVGGGYVQQTGEQFLVQATGLLKGVEDIRRVPVKSLETLKTLRIE"
BASE COUNT          347 a          444 c          442 g          317 t
ORIGIN      
        1 ggaagacctt ttcgaacggg gactcgttca aagcgctctg gtgatcgaag cccatcggca
       61 aatgacggat tacaccgaga gcttcaacgc acaggaactt cgcgccattg aagccctctg
      121 gtcgatctac cgacttgagg gccgcgctct tcaggagaaa atatgaaaat aatgttactg
      181 gtcctgtgtt tgattttatc acttcaactc gcagcggagg aaaagccgaa ccccgcccac
      241 aacgggcacg ccgacgagct cataggccaa gagaaagaga ctcatgctga taacgatgat
      301 gtcggcgaag agagccatgc cgatggagat gagcataagg aagagggcca tgaagaagct
      361 gaagaggcct cctccaacgt tggccccgga aatgcggtga cagcggccga cccggagaga
      421 ggtcttcaac tctcccccga agccttccaa accctggaaa ttcggtcggt ctccgtgccc
      481 gcgaagggcg ttcttcccaa atcggccatc gtcacgttta aagatgaagt cggcgtctac
      541 cgccttcgtg atggttggtt caaattggtc gaggggtccg ctaaaaaccg gaacagcgac
      601 gtcgtgttca tcccccacag atccagcgat ttgcgttcgg gtgattccgt ggttgtccat
      661 ggcgcgccct tgctacgtgt tgcggaactg gacgcctttt ccaccggcga agccggtcac
      721 ggccattaag gcccacagga gaattttatg atcgagaaac tcgtccgttt ttccgttaac
      781 aaccgggggc tcgtgttttt tctggccctc cttttggcgg gactggggtg ggtcagcttc
      841 caggcccttc ccatcgacgc tgtccccgac atcaccaaca accaagtgca ggtcaacacc
      901 gccgtggagg gcctgactcc ggaggagatc gagcgctaca tcaccgttcc cattgaaaac
      961 ggcatggggg gcttggccga tctcattcag acgaggtcca tttcccgaat cggtctttcc
     1021 caggtgaccc tggtttttga ggaccgagtg gacatctacc gcgcccgtca agtggtggcg
     1081 gagcggttgc aaggcgtcat tccccagctt ccggagggga ttttgccggg gctggggcct
     1141 gtcacgacgg ggctgggcga ggtttatttt tacaccatcc aagcctccac cccggcggcc
     1201 aaccccacag agcgggtggc gcagttgatg gaacttaaaa gccttcaaga ctggtattta
     1261 aaaccacggc tcctcacggt ccccggcgtc gccgaagtga acagcatcgg cggattcgag
     1321 aaacagttcc atgtccaacc caacgtccgc atgatggcgc ggtacggcct tcattttagc
     1381 gacttgatcg atgccctgga acgaaccaac cgcaatgtgg gcggcgggta tgtccaacaa
     1441 acgggcgaac agttcctggt gcaagccaca gggcttctca agggcgtcga ggatatacgc
     1501 cgcgttcccg ttaagtccct ggaaacgcta aaaacgctcc gcatcgaaga
//
LOCUS       DAOQLS010000569         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_63151_length_1549_cov_4.696787, whole genome shotgun sequence.
ACCESSION   DAOQLS010000569 DAOQLS010000000
VERSION     DAOQLS010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63151_length_1549_cov_4.696787"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PK362_09545"
     CDS             <1..1388
                     /locus_tag="PK362_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HNC75111.1"
                     /translation="PASSGFILVTVVLILTLLAIFIPVLVRMSTTDSRWSVRRSQIDE
                     AYTVAEAGLDRGSWKLGESESVWQAALAGTAVTEYNYDRTWTDLDSAYRYKIKFSSGP
                     GPTEVRVRCKVQAVRAPTMVRTLEGVYTRNFVEALVMQTDFTGSTDKFPRVHWGAVKS
                     YAALNVNSNVAMPNYPRKLARNGIQGRDTTPDDYNSDRQEYWAYDKNIGDPPVIDYEY
                     YRAKAKASSLPDTTANNGKLRMRHNPAPAATLVKSTPTLGTAYFLCADNWEGAGQDHG
                     IDFDASGGGNHYTFINSTTVVFIETSSNTCRSFIHSEIFLDIEAFIVAGPNNNLNFRP
                     DESAAFAATIPINANMEYLHSAASATWMAGGIGSMDNIWHGSRYPYNISNNIGFHGFL
                     HVQDGELNVDENSHDVKVIGIVYAKQLKIETNPKFFYVYFDDAVASKLKVVYTPIRRR
                     LYREDKADTVW"
BASE COUNT          358 a          474 c          404 g          313 t
ORIGIN      
        1 cccccgcctc gagcggtttt atattggtca cggtggtgct tatcctcacc ttgttggcca
       61 ttttcatccc cgtcttggtt cgaatgagca cgaccgattc ccggtggtcg gtgcgtcgaa
      121 gtcaaatcga cgaagcctac accgtggccg aagccggact ggaccgggga tcctggaagc
      181 tgggggaatc ggaaagcgtc tggcaagccg cgctggcggg aacggccgtg acggaataca
      241 actacgaccg gacctggacc gacctggatt cggcctaccg ttacaaaatc aaattctctt
      301 cgggtccggg ccccaccgag gtccgcgtgc ggtgcaaggt tcaggcggtc cgggcgccca
      361 ccatggttcg gactctggag ggggtctaca cccgaaactt tgttgaggcg ttggtgatgc
      421 aaaccgattt caccggctcc acggataaat tcccccgggt ccactggggg gctgtcaaaa
      481 gctacgccgc gttaaacgtc aattccaacg tggcgatgcc caattacccg cgcaaattgg
      541 ctcgaaacgg cattcaaggc cgcgacacga cgccggacga ctacaactcc gatcgccaag
      601 aatattgggc ctacgataag aacatcggtg accctcccgt gatcgattac gaatactatc
      661 gggcgaaagc gaaggcttcc tcccttccgg acacgaccgc gaacaacggc aaactgagaa
      721 tgcgccacaa tcccgccccc gccgccacct tggtcaaatc cacgccgacc ctgggcaccg
      781 cctatttcct ctgcgcggac aactgggagg gcgccggcca ggaccacggc atcgatttcg
      841 acgcgtcggg gggggggaac cattacacgt tcatcaactc cacgacggtc gtgtttattg
      901 aaacctcctc gaatacgtgc cggagtttta tccactccga gattttcctg gacatcgagg
      961 ctttcatcgt tgcaggcccc aacaacaatc tcaatttccg tcccgacgag tcggccgctt
     1021 tcgccgccac catccccatc aacgccaaca tggaatacct tcattcggcg gcctccgcca
     1081 cctggatggc cggcggcatc ggcagcatgg acaacatctg gcacggttct cggtatccct
     1141 acaacatcag caacaacatc gggttccacg ggtttctgca cgtgcaggac ggggaactca
     1201 acgtggacga aaactcccac gacgtgaaag tcatcggcat tgtctacgcc aaacagctca
     1261 aaatcgaaac caatcccaaa ttcttctatg tttattttga cgacgccgtg gcgtcgaagt
     1321 tgaaggtcgt ctacaccccc atccgtcgtc gattgtaccg ggaagacaaa gccgacacag
     1381 tttggtagat tttcaattcg agttggaaag acgcgacaaa ttaattgccc ttgaattcga
     1441 ttcccccttg aaaaaaacct gaacatcctc cacacttaaa aggcctttta tttcaacaag
     1501 gaggagccat gttcgtcggg aagggatccc gccgcggggc gaccatgat
//
LOCUS       DAOQLS010000570         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_63657_length_1543_cov_4.442876, whole genome shotgun sequence.
ACCESSION   DAOQLS010000570 DAOQLS010000000
VERSION     DAOQLS010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63657_length_1543_cov_4.442876"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1543
                     /gene="dnaE"
                     /locus_tag="PK362_09550"
     CDS             145..>1543
                     /gene="dnaE"
                     /locus_tag="PK362_09550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNC75112.1"
                     /translation="MTTAEFVHLHNHTEYSLLDGATRLTDEKGNPSEFIKAMAAAKFP
                     ALAITDHGNLFGAIEFYQVCTEVGINPIIGIEAYLAPESRLDRKGSPSESAHHITLLS
                     TNLAGYKNLMKLTSQSFLEGYYYKPRLDREILAKHSEGLVALTGCLKGEVSSALLREQ
                     PDKAAKALDDYRAIFGKDNVFVELMDHGLPDQRKVFPRLVELAKKFDAPLVATNDCHY
                     FRKDDHFAHDVLLCIGTGKTLNDPHRLKYNAPEFYYKAPQEMMETFKEVPQALRNTLS
                     IAERCRLSLTFNQILLPRYDVPVGESPEGYLEKLCLEGLKKRFGHIKPEYKTRLDYEL
                     SIIRKMGFSTYFLIVWDFIHYARRQGIPVGPGRGSGAGALTAYALNITNIDPLQNGLL
                     FERFLNPERRSMPDLDIDFSDDGREQVIEYVRQKYGEACVAQIITFGSMLARLVVRDV
                     GRVLDMPLPEVDKVTR"
BASE COUNT          307 a          527 c          447 g          262 t
ORIGIN      
        1 gcgctttccg ccgggcgtcc cgacgtggcg gcggcggcgc ggcgccggct ggtgaacccc
       61 cgggcccccg ccgacggacc gaaaccgacg gtccgcgaca acccggcgtc aaacggctat
      121 aatgcgctct cccgttaaaa acccatgacc accgccgagt tcgtccacct ccacaaccac
      181 acggagtaca gcctgttgga cggcgccacg cgcctgaccg acgaaaaagg caacccctcg
      241 gaattcatca aggccatggc cgcggcgaaa ttccccgctt tggccatcac ggaccacggc
      301 aacctgttcg gcgccatcga gttttaccag gtctgcaccg aggtggggat caaccccatc
      361 atcggcatcg aagcctatct ggcgcccgag tcccgcctgg accggaaggg cagcccctcg
      421 gagtccgccc accacatcac gctgctgtcc acgaacctcg ccggttacaa aaacctgatg
      481 aagttgacgt cccagtcctt cctggaaggc tactactaca agccccgcct ggaccgggaa
      541 atcctggcga aacattccga gggcctggtg gccctcacgg gctgtctcaa aggcgaggtg
      601 tcgtcggcgc tgttgcggga gcagccggac aaggccgcca aggccctgga cgattaccgg
      661 gcgatcttcg ggaaggacaa cgtgttcgtg gagttgatgg accacggcct gccggatcag
      721 cggaaggtgt ttccccgcct cgtggaattg gcgaaaaagt tcgacgcgcc cctcgtggcg
      781 accaacgact gccattattt ccgcaaagac gaccatttcg cccacgacgt gctcctgtgc
      841 atcggcaccg ggaaaaccct gaacgatccc caccgcctga aatacaacgc cccggagttt
      901 tattacaagg cgccccagga gatgatggaa accttcaagg aggttcccca ggcccttcgg
      961 aacaccctgt ccatcgccga acgctgccgc ctgtccctga ccttcaacca gattctcctg
     1021 ccccgctacg acgtgccggt gggcgagtcg ccggaggggt acctcgagaa gctgtgcctg
     1081 gagggcctca agaaacggtt cgggcacatc aagccggaat acaaaacccg gctggattac
     1141 gaactgtcga tcatccgcaa aatgggtttt tccacctatt tcctgattgt ctgggatttc
     1201 atccactacg cccgccgcca gggcatcccc gtggggcccg gccgcggctc gggcgccggg
     1261 gccctcacgg cctacgccct gaacatcacc aacatcgacc cccttcaaaa cgggttgctc
     1321 ttcgagcggt tcctgaaccc cgaacgccgc tccatgcccg acttggacat cgatttttcg
     1381 gacgacggtc gggagcaggt catcgaatac gtccgccaga aatacggcga ggcctgcgtc
     1441 gcgcagatca ttacgttcgg ctccatgctg gcgcggctgg tggtccggga cgtcgggcgg
     1501 gtgctggaca tgccgctccc ggaagtggac aaggtgaccc ggc
//
LOCUS       DAOQLS010000571         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_63734_length_1542_cov_3.681910, whole genome shotgun sequence.
ACCESSION   DAOQLS010000571 DAOQLS010000000
VERSION     DAOQLS010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63734_length_1542_cov_3.681910"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..615)
                     /locus_tag="PK362_09555"
     CDS             complement(79..615)
                     /locus_tag="PK362_09555"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNC75113.1"
                     /translation="MTVSATPGLDLKRFIRDIPDFPKKGIVFKDITPLLADGAAFSKT
                     IDLLAAAGRRQKATKIVAIESRGFIFGSAVAQALGVGVVPVRKRGKLPFTTISTTYAL
                     EYGTDALEMHVDALSAGDRALVVDDVLATGGTAEAVVRLIQKAGGNAVGLAFVIELDF
                     LSGRAKLPNLDVTALIRF"
     gene            complement(612..1442)
                     /locus_tag="PK362_09560"
     CDS             complement(612..1442)
                     /locus_tag="PK362_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC75114.1"
                     /translation="MRESLDAVTLYFHGVKRLQDVSKDDVSALWRAAKRGDKKAKRRI
                     VETNLRLVIPIAKKYYRPGVDFLDLIEEGNLGLMHAVDKFDPKRGFRFSTYAAYWIEQ
                     AVRRAYDEQSKTIRIPPHALEALRKWLREWDRMSISLGRNPTLQEMGKKLNLSARQIK
                     SVIDAHEAAKSVGSLDTPLDEDENLFIRDIVTDRSDQGPDEIFSLLRVRDDLAHALIQ
                     IGPREKSILDMRFGLTGRDPMTLEEVGKKLRLSRERVRQLEERGLARLRRVAQRMGLI
                     "
BASE COUNT          303 a          488 c          444 g          307 t
ORIGIN      
        1 tgaaccacca accttctcct taggacggag ccgctctatc cagttgagct acgggggctc
       61 ggaggtggag cggaaaggtt aaaaccgaat cagcgcggtc acgtccaagt tcggcagttt
      121 ggcgcgtccg ctcagaaaat cgagctcaat cacaaaagcc agccccacgg cgttcccgcc
      181 ggccttttga atcagtcgaa cgacggcctc ggcggtgccg cccgtggcca agacgtcgtc
      241 caccaccagg gcgcggtccc cggcggacag ggcgtccacg tgcatttcga gcgcgtcggt
      301 tccgtattcc aaggcgtagg tcgtggagat cgtcgtgaag ggaagtttcc cccgtttgcg
      361 aaccggaacg acgccgaccc ccagcgcctg agccacggcg gacccgaaaa tgaaaccccg
      421 ggattcgatg gcgacgatct tcgtggcttt ttgacgccgc cccgcggccg ccagcagatc
      481 gatggttttg gaaaaagccg ccccgtcggc cagaagcgga gtgatgtctt tgaaaacgat
      541 gccttttttc gggaagtccg gaatgtcgcg aataaaccgc ttgagatcca atccgggggt
      601 ggccgagacc gtcatatcaa tcccatccgc tgggccacgc gccgcagccg cgccaatccg
      661 cgctcttcca attgacgaac gcgttcccgc gacaatcgaa gtttcttacc gacttcctcc
      721 agcgtcatgg gatcccgtcc ggtcagcccg aaacgcatgt ccaaaatgct cttctcccgg
      781 ggtccgattt ggatgagggc gtgggccaaa tcgtcccgca cgcgaagcag cgaaaagatt
      841 tcgtccggcc cctggtcgga ccgatccgtg acgatgtccc ggatgaaaag attttcgtct
      901 tcgtcgagag gcgtgtccag gcttcccacc gacttggccg cctcgtgggc gtcgatgacg
      961 cttttgatct ggcgggccga caaattcaat tttttgccca tttcctgaag ggtcggattc
     1021 cggcccagac tgatcgacat ccggtcccat tcccgcaacc atttgcgcag ggcctcgagc
     1081 gcgtggggcg ggatgcggat ggttttggat tgctcgtcgt acgcccgacg gacggcctgt
     1141 tcgatccaat aggcggcgta ggtcgaaaag cggaaccccc gtttcggatc gaacttgtcc
     1201 accgcgtgca tcaaccccag gttgccctct tcgatcaagt cgaggaagtc gacgcccggg
     1261 cggtaatatt ttttcgcgat gggaatgacg agccggagat tggtctcaac gatgcgtcgt
     1321 ttcgcctttt tatcgcctct cttcgccgcg cgccagaggg cggacacatc gtctttggaa
     1381 acatcctgaa gtcgtttgac gccgtgaaaa taaagggtaa ccgcgtccaa gctttctctc
     1441 atgatcctcc cgccgagtca acccgcgatg tcaaaaccct gatcccgcgc cgcgccggcc
     1501 gaacaccgca cgacgtccac acgctccacc cgcgcccaag gg
//
LOCUS       DAOQLS010000572         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_64246_length_1536_cov_3.024983, whole genome shotgun sequence.
ACCESSION   DAOQLS010000572 DAOQLS010000000
VERSION     DAOQLS010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64246_length_1536_cov_3.024983"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PK362_09565"
     CDS             complement(<1..1404)
                     /locus_tag="PK362_09565"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HNC75115.1"
                     /translation="MTSVSHRPALLITTLAEYQTAFWLRVGLALRDRGVHALFLTYDD
                     RSHERLRAAGLPSLNAFDAARAAGAAAGAPAFDEWVHRLGVADPNLWMSHERVAFGLR
                     DGVELRRKFCAGAAAAEAAVALLRDEGRTPVVVQELGGFLSVIGAFFVARARGLDHYF
                     IEPSFYKGRFLFLKNTFAAQKFAPEPAAELTPEVRRLLEKTRASQSLVIPLKDKHHYA
                     APERKILNARNVKRFFQKWWDRRMGKRQEFGYLGAHVAGHLRMVANGWRLKGSYTPLN
                     ALGRFVYYPLHVPGDVALTLRAPDYFDQLALIDYLARTVPVSHRVAFKEHPAMIGALD
                     AGRIRSLLRRHDNVFLLSPDTNNYDVLNAAEAVVTVNSKSGAEALMLGKPVFVLGDAF
                     YRQAPSVRRIDHLAQLPGALRALFQGPVAPPDGRAVERFFQEVWNATWPGELYQPTDQ
                     AAARFADSLVHAVAADRG"
BASE COUNT          298 a          484 c          519 g          235 t
ORIGIN      
        1 ccctcggtcg gccgccacgg cgtgaacgag cgaatcggcg aatcgggcgg cggcctggtc
       61 ggtcggctgg tagagctcgc ccggccacgt cgcgttccac acttcctgga aaaaccgctc
      121 cacggcgcgg ccgtcgggcg gtgccaccgg cccctggaaa agggcgcgga gggcgcccgg
      181 cagttgggcg aggtgatcga ttcggcgaac cgaaggcgcc tggcggtaga aagcgtcgcc
      241 caaaacgaaa acgggcttcc cgagcatcag cgcctcggcc cccgatttgg aattgacggt
      301 gacgaccgcc tcggcggcgt ttaatacgtc gtagttgttg gtgtccgggg acaaaagaaa
      361 aacgttgtcg tgtcggcgca ggagggaccg aatgcggccg gcgtccaagg cgccgatcat
      421 ggccggatgc tccttgaacg ccacccggtg ggaaacggga acggttcggg cgaggtaatc
      481 gatgagcgcc agttggtcga aataatccgg ggcccgcagg gtgagcgcca cgtcgccggg
      541 gacgtgcaac gggtaataca caaaccgtcc caaggcgttc aagggcgtgt aagacccctt
      601 caaccgccag ccgttggcga ccatgcgaag atgacccgcg acgtgggccc ccagatagcc
      661 gaattcctgc cgtttcccca ttcgacggtc ccaccatttc tggaaaaacc gcttgacgtt
      721 gcgggcgttg agaatttttc gttccggcgc cgcgtaatgg tgcttgtcct tcagggggat
      781 gaccaaggat tggctggcgc gggttttttc caggaggcgg cggacctccg gcgtcagttc
      841 ggcggcgggc tccggagcga acttctgggc ggcgaaggtg tttttgagga aaagaaaccg
      901 ccctttgtaa aacgacggct cgataaaata gtggtcgagc ccgcgggccc gcgcgacgaa
      961 aaacgcgccg atcaccgaca ggaacccgcc caattcctga accacgaccg gggttcggcc
     1021 ctcgtcgcga aggagcgcca cggccgcttc cgcggccgcc gcgccggcgc aaaatttccg
     1081 ccgcagttcg accccgtcgc ggaggccgaa ggcgacgcgt tcgtggctca tccaaaggtt
     1141 cggatccgcc acccccagcc ggtgaaccca ttcatcgaaa gcgggggcgc ccgcggccgc
     1201 ccccgcggcc cgggcggcgt caaacgcgtt caaggacgga agacccgcgg cccgaagccg
     1261 ttcgtggctg cggtcgtcgt aagttaaaaa aagcgcgtga accccccggt cccgaagcgc
     1321 gaggccgacg cggagccaga aagccgtctg gtactcggcg agggtggtga tcaacagcgc
     1381 gggtcgatgg gacacggaag tcatggcgat ctctgggacg gcccccgacg gggaaacgat
     1441 ccttgggaat ttaacgcaag gcggccccgc caagcggcgg ggccccggtc gcccattcta
     1501 ccaaaccttt attcgacgga aaagcgccct tccacg
//
LOCUS       DAOQLS010000573         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_65089_length_1526_cov_2.970088, whole genome shotgun sequence.
ACCESSION   DAOQLS010000573 DAOQLS010000000
VERSION     DAOQLS010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65089_length_1526_cov_2.970088"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PK362_09570"
     CDS             complement(<1..755)
                     /locus_tag="PK362_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75116.1"
                     /translation="MESGSLSGTLIGAADLAVLLGLLALMVGMGFFFRRGQGTAEDFF
                     LGGRRLPIWAACLSFVATEVSAVTVISVPATACNENWQYLQFFIGSTAARVVIAFLFI
                     PAFYKFNCTTIYQFLGARFGRVTQTAASFFFFLTRLLGSGVRLMAACLALSVLLNTPI
                     LPVIAVFVLVGVAYMSLGGMKAVVWTNVIQAMVFIGGGLATLAFLGHTVAGGWPAIWS
                     QAGAAGHTAILEWGPARGTPHFWRAFFANPNIF"
     gene            complement(758..>1526)
                     /locus_tag="PK362_09575"
     CDS             complement(758..>1526)
                     /locus_tag="PK362_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNC75117.1"
                     /translation="LEEAARADKEFIVLDRPNPLGGAAVEGLVLSTEIRHFTAYYSIP
                     VRHGLTVGELAQWHDAHAGLNARLMVIPVKGWRRSMLWDETGLPWVPPSPNIKTPRAA
                     LLYCGVGAFESTNVSVGRGTATPFEVFGAPWLKGVPLAKKLNALKMEGVRFRSATFRP
                     TSDRYRGERCYGVRVLVDDPRRARPVDLFVNAACLLRDARPAAFRPRWAEMPRVVGSG
                     DFEIQFNGGASAADLLAVAHRSAAAFDAERRPHLLYR"
BASE COUNT          342 a          512 c          470 g          202 t
ORIGIN      
        1 cagaagatgt tggggttggc gaaaaacgcg cgccaaaagt ggggcgtccc ccgagccggt
       61 ccccattcca gaatcgccgt gtgcccggcg gcgccggctt gggaccaaat ggccggccaa
      121 ccgcccgcca cggtgtggcc caaaaaggcc agcgtggcca gcccgccgcc gatgaacacc
      181 atcgcctgga tcacgttcgt ccacacgacg gccttcatgc ctcccaacga catgtaggcg
      241 acgccgacca ggacaaagac cgcgatcacc ggcaaaatcg gcgtgttcaa caaaacggac
      301 aacgccaggc aggcggccat caaccgcacg cccgagccca acaaacgcgt gaggaaaaag
      361 aaaaaggagg ccgccgtttg cgtgacgcga ccgaaccgcg ccccgagaaa ttgatagatc
      421 gtcgtgcaat tgaatttgta aaaagccgga atgaacagga aggcgatcac cacacgggcg
      481 gcggtcgaac cgatgaagaa ctggagatat tgccaatttt cattgcaggc cgtggccgga
      541 acggaaatga ccgtcacggc gctcacttcc gtggccacga acgaaaggca ggcggcccaa
      601 atcggcagcc gacggccgcc gagaaagaaa tcctcggccg tcccctgccc ccgtcgaaag
      661 aaaaaaccca tccccaccat caacgccagg aggcccagaa ggacggcgag atccgccgcg
      721 ccgatcaacg tcccgctcaa ggacccggat tccatcgtca gcggtaaagc aggtgggggc
      781 gccgctcggc gtcgaacgcc gcggcgcttc gatgggcgac ggccaacagg tccgcggcgc
      841 tcgcgccccc gttgaactgg atttcaaaat cgccgcttcc cacgacccgg ggcatttccg
      901 cccaacgggg ccggaaagcc gcgggtcgcg cgtcgcgaag gaggcaggcg gcgttcacga
      961 agagatccac cggccgggcc cggcggggat cgtcgacgag aacccgaacg ccgtaacagc
     1021 gctcgccccg gtaacggtcc gacgtcggcc ggaacgtggc cgagcgaaaa cgaaccccct
     1081 ccattttcaa ggcgttcagt tttttcgcca acggcacccc cttcaaccag ggggccccga
     1141 aaacctcaaa gggggtggcc gtcccccggc ccaccgacac gttcgtcgat tcaaaggcgc
     1201 cgaccccgca atacaacaac gccgcccggg gcgttttaat gttcggggac ggcggaaccc
     1261 agggcaagcc cgtttcgtcc cacagcatgg atcgccgcca ccctttcacg gggatgacca
     1321 tcaagcgggc gttgagaccg gcgtgggcat cgtgccattg ggccagttcc cccaccgtca
     1381 atccgtggcg gaccggtatg gaatagtaag cggtgaagtg acggatctcg gtggacagca
     1441 ccaggccttc gacggcggcg ccgcccaggg gattggggcg gtccaacacg ataaactcct
     1501 tgtcggcccg agcggcttcc tccagg
//
LOCUS       DAOQLS010000574         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_65872_length_1517_cov_3.341997, whole genome shotgun sequence.
ACCESSION   DAOQLS010000574 DAOQLS010000000
VERSION     DAOQLS010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65872_length_1517_cov_3.341997"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PK362_09580"
     CDS             complement(<1..531)
                     /locus_tag="PK362_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC75118.1"
                     /translation="MIIERNVAKYVIFSDDSLVNALRRISENKAGFVFALTETGIVEG
                     VLTDGDVRRWLVAQKNIDMDAPVSTVANRQFVSAPDTDTAEKIEDRLTDRIRYVPVLD
                     RQGRLVGVASRKAAEVRLGDFVIADSSPAFVIAEIGNNHNGRMDLARRLVDEAVAAGA
                     HCAKFQLRSMDTLYRKN"
     gene            complement(528..1214)
                     /locus_tag="PK362_09585"
     CDS             complement(528..1214)
                     /locus_tag="PK362_09585"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNC75119.1"
                     /translation="MTRYAAVLPARAGSKGIPGKNLKAIAGKPLLAWSIEAALACGRL
                     SDVVVSSDGDELLALAGRWGAAALRRPAALATDAAATEPTLVHAVQWLESAGRRPDAV
                     VLLQPTSPLRRAGTLDKALDLFEKSGADSLLTVCESHAFFWKNPAAPEALYDFRHRPR
                     RQDIPAADRWYRENGSIYVTRTDVLLRENNRLGGRIALLPVSEEESVEIDSEADFAIV
                     EKLLERERPS"
BASE COUNT          266 a          494 c          487 g          270 t
ORIGIN      
        1 gttcttccga tagagggtgt ccatggaccg cagctggaat ttggcgcaat gggcgccggc
       61 ggcgacggct tcgtccacca ggcgccgagc caaatccatc cggccgttgt ggttgttgcc
      121 gatttcggcg atgacgaagg cgggcgaaga atcggcgatg acaaagtccc cgaggcggac
      181 ttcggccgcc ttccggctgg cgacgccgac caggcggccc tgccggtcca gcacgggcac
      241 gtagcggatc cggtcggtga ggcggtcctc gattttttcc gcggtgtcgg tgtccggcgc
      301 cgaaacgaac tgccgattgg ccacggtcga cacgggggcg tccatatcga tgtttttctg
      361 ggcgaccagc catcggcgga cgtcgccgtc ggtcagcacc ccttcgacga tgcccgtttc
      421 cgtcaaggcg aaaacaaaac cggctttgtt ctcgctgatg cgccgcaggg cgttgaccag
      481 cgaatcgtcc gaaaagatga cgtatttggc gacgttgcgt tcgatgatca cgacggtctc
      541 tctcgttcca aaagtttttc gacgatggcg aaatcggctt cggagtcgat ttcgacgctt
      601 tcctcttcgc tcacgggcaa aagcgcgatc cgcccgccca gacggttgtt ttcccgcaac
      661 aagacgtcgg tgcgggtcac gtaaatcgag ccgttctccc ggtaccaacg atcggccgcc
      721 ggaatgtcct gccgccgggg ccggtggcga aaatcgtaga gggcctccgg cgcggccggg
      781 tttttccaaa aaaacgcgtg actttcgcag accgtcagca aggaatcggc tccgctcttc
      841 tcgaacaagt cgagggcttt gtccaaggtt cccgctcggc ggaggggcga cgtcggctga
      901 agcaagacca ccgcgtccgg ccgccgaccc gcgctttcca gccactggac ggcgtgcacc
      961 aaggtcggtt ccgtggccgc cgcgtccgtc gccaaagcgg ccgggcgccg cagggccgcg
     1021 gcgccccaac ggccggccaa cgccagcagt tcgtccccat cggaggaaac cacgacgtcg
     1081 ctcaagcgtc cgcaggccag cgccgcctca atgctccacg ccaagagcgg tttgccggcg
     1141 atcgctttca gattttttcc cgggatcccc ttggagccgg cccgggccgg aaggacggcc
     1201 gcgtaccggg tcatgacggg aaggcggtct tcggcgaagg cgtcatagtg gattcatcct
     1261 attgtcctat atttcgcgcg gtgattccac cgaatcgaca ccgatcaaac aagtgggatg
     1321 ccaacggttt cggtgggcgg gaaggagcga attcagcgcc cggaacgggg caacgggacg
     1381 ggggccgaag cccccgcggg cggcgcgtat tccggcacca accgcttcat ttcggccttc
     1441 acctccgcgt cgggcactcg gacgtcggcg tcgacccatc ccaccacgta atccaaccag
     1501 ccggcgggca ccgtccg
//
LOCUS       DAOQLS010000575         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_66197_length_1513_cov_4.184499, whole genome shotgun sequence.
ACCESSION   DAOQLS010000575 DAOQLS010000000
VERSION     DAOQLS010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66197_length_1513_cov_4.184499"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK362_09590"
     CDS             <1..322
                     /locus_tag="PK362_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNC75120.1"
                     /translation="APFQLENIRFNTWHPEDEDVVKFLRLVASPDRLPLLVHCQHGSD
                     RTGTMVAIYRIAVQGWSKESALDEMVNGGYGFHPLWKNLRRYIRNLDVAAVKAKAAAD
                     GPWR"
     gene            333..551
                     /locus_tag="PK362_09595"
     CDS             333..551
                     /locus_tag="PK362_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75121.1"
                     /translation="MKKVLLWALLGFVFFLVYLFQPVCVPLTDEDLKSFNGPIEQRTD
                     RDFYLKVFQKKNGRWHQCKTRVSRLFFS"
     gene            562..948
                     /locus_tag="PK362_09600"
     CDS             562..948
                     /locus_tag="PK362_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019028297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNC75122.1"
                     /translation="MKFLGRLSEPAYALLRLATGFLFCFHGAQKILGVFSQFRPAPFT
                     QLWYGGLIELVGGFLVLTGLQTRAAAFLCSGTMAVAYFQFHWKGQWGARFFPAVNEGE
                     LAALYCFVFFFIACRGAGKWGLGKGD"
BASE COUNT          283 a          418 c          451 g          361 t
ORIGIN      
        1 cgcccccttt caattggaaa acattcggtt caatacctgg catccggagg acgaggacgt
       61 ggtgaaattc ctccgcctcg tcgcttcgcc ggatcggctg ccgttgctgg ttcattgcca
      121 gcacggatcg gatcgaaccg gcaccatggt cgccatctac cggatcgcgg ttcaagggtg
      181 gtccaaggaa agcgccttgg atgaaatggt caacggcggc tacggattcc accccctttg
      241 gaaaaacctc cgtcgttaca ttcgaaactt ggacgtcgcc gccgtcaaag ccaaggccgc
      301 cgcggacggc ccctggcgat aggtgctttc ccgtgaaaaa ggtcctcctt tgggcgttgt
      361 tgggctttgt tttcttcctg gtttatcttt tccaaccggt gtgcgttcct ttaacggacg
      421 aggatctgaa atcgttcaac ggtcccattg aacaacggac cgatcgggac ttttatctaa
      481 aggtttttca aaagaaaaac ggacgctggc accaatgcaa aacgcgggtg tcccgacttt
      541 ttttctccta gaggaggacc catgaagttt cttggaagat tgtcggaacc ggcctacgcc
      601 ctgcttcgct tggcgaccgg atttttgttt tgctttcacg gggcccaaaa aatcctgggg
      661 gtcttttccc aattccgccc ggccccgttc acccaactgt ggtatggggg gttgattgaa
      721 ctcgtgggcg gatttttggt cctgacgggc cttcagaccc gcgcggcggc cttcctttgc
      781 agcgggacca tggcggtggc ctattttcag ttccactgga aaggccagtg gggggcgcgg
      841 ttcttccccg ccgtgaacga aggcgaattg gccgccctct attgttttgt gttttttttc
      901 atcgcctgcc ggggggccgg gaaatggggt ttgggaaagg gcgactaggg gttttccttc
      961 catgaatgcg tccgggcgtt gggtccggcg cggatggatc gcggggtgga ttttgacgct
     1021 gatgggggga tcgatcgcct gccgatccgg tcgcgaggcc tcgaagaaca tcgaccccga
     1081 accgggcaac aaaggaaagt gtggggaaat tcgttcggcc ctttcgattt attattccga
     1141 caatcaaggg attttccccg cccaccttga ttcgctggtc cccaaatacc tttccgaaat
     1201 cccgacttta aatggaatcg gaaaccaccc gccctcccgc gcggtcgcgg tggtctccgc
     1261 gccacgatca tcggataccg gtggatggat ttacgtgttt aatcccgcgt cgccccggca
     1321 cggggaggtg tctatcgatt gcacccacag cgatggaaag gcgccctgga gttcgttttg
     1381 agggcgttgc cgaccgggga aggaggccgg gccgacgtgg agcgcgggta ttaggcggcg
     1441 gtcgacaaat cccggaaacc gatcaacttt ttacggtttt cgtcccacag actcagccgg
     1501 atgcacttga ggc
//
LOCUS       DAOQLS010000576         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_66248_length_1513_cov_2.361454, whole genome shotgun sequence.
ACCESSION   DAOQLS010000576 DAOQLS010000000
VERSION     DAOQLS010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66248_length_1513_cov_2.361454"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1513)
                     /locus_tag="PK362_09605"
     CDS             complement(<1..>1513)
                     /locus_tag="PK362_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75123.1"
                     /translation="PVDDDLKKGFPRGASGEGPVRYRVDPGKSLFEVVVDRESFPDVF
                     GGVVRAFSSIGPRSYAVGMVERFSEGLLRPGGKAYETRLLLSGRVGDRSVEWQKEARD
                     EGDFRQPGSFARHGVSGFFAGEGGLEEDLPYPRLFDPLAAIVPSLRTPAGRAEFESRL
                     QGELDRIFEGVGARLLALGVPRGFLISMELDLMWLPAPDGGYPRPLLLESKFYPKFES
                     ENDRERRLDVAVLPRDTTSPNSILYRLVRLTAAGLSRAPADSAAHRVGRWLDRQSPRW
                     GLWGLTGEIPGLMAASLVLGPVGALAGLAVFTVLHLLLEALELHLARGDLSSAPASGK
                     SILARVFAQNLRPQFLAFVLAYGPLAHFGPAPAALAGAILVHLSYDVYRMFPAPFRAL
                     FPRRVKPAVPTTAAKGEWIPAVLSPAAAAGLARQLRVRIDAYAKNELPQDWYLSEVRP
                     YRVALLWNIAAAYYLLESRSPGYFADQWADESPALRDRVAAVLEEIQVFDRERG"
BASE COUNT          329 a          499 c          487 g          198 t
ORIGIN      
        1 acccgcgttc ccgatcgaag acctgaattt cctccaacac cgcggcgacc cgatcccgaa
       61 gggccgggga ttcgtcggcc cattggtcgg cgaaatatcc cggcgaccgg ctttccaaca
      121 agtagtacgc ggccgcaata ttccaaagga gcgccacccg gtacggacgg acttcgctga
      181 ggtaccaatc ctggggcaat tcgttcttgg cgtaggcgtc gatccgaacc ctcaattgac
      241 gggccagccc ggcggccgcg gccggggaca ggaccgccgg aatccattcg cctttcgccg
      301 cggtcgtcgg aacggcgggt ttcacccgcc gggggaaaag ggcccggaag ggggccggga
      361 acatccggta tacatcgtag gacaggtgaa cgagaatcgc cccggcgagg gcggcggggg
      421 ccggaccgaa atgggccagg ggcccatagg caagaacgaa cgcgagaaat tgggggcgaa
      481 gattttgggc gaagacgcgg gccaggatcg acttcccgga cgccggagcg gaactcaaat
      541 cgccccgggc cagatgaagc tcgagcgcct ccagtaaaag atggagaacg gtgaaaacgg
      601 ccagtcccgc caaagccccg accgggccca gaaccaaaga cgccgccatc aatcccggaa
      661 tttccccggt caagccccaa aggccccagc ggggactttg ccgatcgagc caccggccga
      721 cccggtgggc ggccgagtcc gcgggggccc gggaaagccc ggcggcggtc aaacggacca
      781 accggtaaag aatcgaattc ggggaggtcg tgtcccgggg aaggaccgcg acgtccagcc
      841 gccgctcccg atcgttctcc gattcgaatt tgggatagaa cttcgattcc agcaacaacg
      901 gccggggata cccgccgtcg ggggcgggaa gccacatcaa atccaattcc atggaaatca
      961 aaaatccccg gggaacgccc agggccagca accgcgctcc cacgccttca aaaattcgat
     1021 ccaattcccc ctgcaggcgg gattcgaatt ccgcccggcc cgcgggcgtt cgaagggagg
     1081 gaacgatcgc ggccaagggg tcgaaaaggc gcgggtaggg aagatcctcc tccaggccgc
     1141 cttcgcccgc gaagaaaccc gacacgccgt ggcgggcgaa ggacccgggt tggcggaaat
     1201 cgccctcatc ccgagcctct ttttgccact cgacggaccg gtcccccacc cgaccggaga
     1261 ggagcaaccg ggtctcgtaa gccttgcccc ccgggcgaag caggccttcg ctgaaacgct
     1321 ccaccatgcc gacggcgtag gagcgcgggc caatgctgga aaaagcccga accacgcccc
     1381 caaagacgtc ggggaaggac tcccggtcga cgacgacctc aaaaagggat ttccccggat
     1441 ccacgcggta gcgaaccggt ccttcgcccg aggcgccccg gggaaatccc tttttgaggt
     1501 cgtcgtcgac cgg
//
LOCUS       DAOQLS010000577         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_66300_length_1512_cov_3.680165, whole genome shotgun sequence.
ACCESSION   DAOQLS010000577 DAOQLS010000000
VERSION     DAOQLS010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66300_length_1512_cov_3.680165"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..957)
                     /locus_tag="PK362_09610"
     CDS             complement(280..957)
                     /locus_tag="PK362_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNC75124.1"
                     /translation="MTVLLAERPVRTATADALLEGDLYLPDEPRGLIVFAHGSGSSRH
                     NARDRHVVQRLAAEGFGTLVFDLLTRSEDAQDRWTYRFRFDTPLLSRRLTSALDWARR
                     SPVSRHLKIGLLGAASDAGAALRVAAERTRDVAAVVLLGGRPEASRDILRRVSAPTLM
                     LVGEKDNAVMLQSRHALDQFHGPRELSVVPGASHLFLEGDTLEVAARAASDWFARHLT
                     PHARSAA"
     gene            complement(954..1115)
                     /locus_tag="PK362_09615"
     CDS             complement(954..1115)
                     /locus_tag="PK362_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNC75125.1"
                     /translation="MDICPVCGQSVDRLEAYLAEFSGDVRFFCSRACLEAFERDPARY
                     VAVPREAER"
     gene            complement(1178..>1512)
                     /locus_tag="PK362_09620"
     CDS             complement(1178..>1512)
                     /locus_tag="PK362_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC75126.1"
                     /translation="LRMAPLARWMKPGDTDPAAWVGRMVEESCARLQGKGLLVSGRVE
                     KGNPKTVLVEKAAKWRAHGLYLGARGLTRWERFMLGSVSTAVAARAACSVEVVRGPRR
                     ETALTGPA"
BASE COUNT          257 a          509 c          514 g          232 t
ORIGIN      
        1 tcggcgtctc tgacgccgcc tgccgtcccc aaagctctgc gtgggggcgg cttccgcatt
       61 cttcggcgtc tctgacgcgg cctgccgtcc ccaaagctct gcgtgggggc ggcttccgca
      121 ttcttcggct tctttggccc cgcctgccgt ccatgcctcc ggaaaaggca ggggacacgg
      181 tttccgttgg ggaattcgcc atcggaaccc tcaaatatta caccactttt cgacggattc
      241 aaccgccttt tcggcgtgga tttttggagg ggaaagaggt taagcggccg accgggcgtg
      301 gggggtgagg tggcgggcga accaatcgga ggccgcccgc gcggccactt caagggtgtc
      361 gccttccaaa aacaggtggg acgccccggg cacgacggac aactcccggg gtccgtggaa
      421 ctgatccaag gcgtgacggc tttggagcat cacggcgttg tccttctccc ccaccagcat
      481 gagggtcggg gcggaaaccc gccggagaat atcccgggag gcctcggggc gtccgcccag
      541 gagaaccacg gcggcgacgt cccgggtccg ttcggcggcc acgcgcaggg ccgccccggc
      601 gtcggacgcc gcccccagaa gaccgatttt taggtggcgg gaaaccggcg aacggcgcgc
      661 ccaatccaag gcggaggtca aacggcggga caggagcggg gtatcgaaac ggaaacggta
      721 ggtccatcgg tcctgggcgt cttcggagcg ggtcagaaga tcgaacacca gggtgccgaa
      781 cccttcggcg gccaggcgtt ggacaacgtg gcggtcacgg gcgttgtggc gggaggatcc
      841 ggacccgtgg gcgaagacga tcaacccccg gggctcgtcg ggaaggtaca aatccccctc
      901 gaggagggcg tcggccgtgg ccgttcgaac gggacgttcg gcgagaagga cggtcatcgc
      961 tcggcctccc ggggcaccgc cacgtaacgg gcggggtccc gctcgaaggc ctcgaggcag
     1021 gcccgggaac aaaagaaccg gacgtccccg ctgaactccg cgaggtacgc ttcgagccga
     1081 tccaccgact gaccgcacac gggacagatg tccatggccg cctccacccg tgactctagc
     1141 tctcccccct cggatccgcc atgacggcgg tcaggtttta cgccgggccg gtgagggcgg
     1201 tttcccggcg gggaccgcgg accacttcga cggagcaggc ggcccgggcc gccacggcgg
     1261 tcgacacgct gccgagcata aaccgttccc agcgggtgag accccgggcc cccagataca
     1321 ggccatgggc ccgccatttg gcggcttttt ccaccaacac cgtcttggga ttcccctttt
     1381 ccacccgccc ggacacgagc aaaccttttc cctgcaggcg ggcgcaggat tcctccacca
     1441 tgcggcccac ccaggccgcg ggatcggtgt ctcccggttt catccaacgg gccagaggcg
     1501 ccatccgcag gg
//
LOCUS       DAOQLS010000578         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_66380_length_1511_cov_3.926511, whole genome shotgun sequence.
ACCESSION   DAOQLS010000578 DAOQLS010000000
VERSION     DAOQLS010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66380_length_1511_cov_3.926511"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PK362_09625"
     CDS             <1..837
                     /locus_tag="PK362_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC75127.1"
                     /translation="KRVRPGLVFVASRFGSADPAAVRETALAVELIHIATLVHDDLVD
                     DALMRRQRPTVGVKFGEGAAVLLGDYVYAQAFQRLGALGRPDLVRLYADTTMVMCEGE
                     IGQYESRYRFTLSESEYLDFLGKKTASLMAAACRSGALLAGLNEAQARALETFGEKLG
                     VAFQVVDDLLDVEGDEAVVGKTLHTDLTHGKMTLPLILYSARLSSDKERQALFELLRN
                     PNGHLRGLIERVRASGVLADCRTKVQQLLNEADGALKALPDVPARRLLADIALRLSSR
                     QV"
     gene            899..1081
                     /gene="tatA"
                     /locus_tag="PK362_09630"
     CDS             899..1081
                     /gene="tatA"
                     /locus_tag="PK362_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015116966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNC75128.1"
                     /translation="MFGMGWQELLLVLVIMLLFFGPNKLPALGKSLGKALAGFKQGMK
                     EGAEEADKGLPEDPKA"
     gene            1111..>1511
                     /locus_tag="PK362_09635"
     CDS             1111..>1511
                     /locus_tag="PK362_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNC75129.1"
                     /translation="MTAPAAPPMDRPSDTPGDRARPLVDHLTEIQRRLRWSLAAVLAL
                     SVAGWFLTSPIIHRLARSVGPMIYLAPTEAFAVRFKMAVLLGVFLSAPVLIYHLWRFV
                     GVALTLSERRVVLGALPFSYLLFVVGASLGW"
BASE COUNT          240 a          501 c          452 g          318 t
ORIGIN      
        1 aaacgcgtac ggcccggtct ggtttttgtg gcctcccgat tcggttcggc cgaccccgcc
       61 gcggttcggg aaacggccct cgccgtcgag ctgattcaca tcgccacctt ggtccacgac
      121 gacctggtgg acgacgccct gatgcggcgc caacgcccca cggtcggcgt taaattcggc
      181 gagggcgccg cggtgctcct cggcgattac gtttacgcgc aggcttttca gcgcctcggc
      241 gccttgggac gtccggacct ggtgcgcctc tacgccgaca cgaccatggt catgtgcgag
      301 ggggaaatcg gccaatacga aagccgttat cgtttcaccc tctccgaatc cgaatacctg
      361 gatttcctcg gcaaaaaaac ggcctccctg atggccgccg cctgccggtc gggcgccttg
      421 ctcgccggtt taaacgaagc ccaagcccgg gccctggaaa ccttcggcga aaaattgggc
      481 gtggcgttcc aggtggtcga cgatctgttg gacgtggagg gggacgaggc cgtcgtcggt
      541 aaaacgcttc acacggattt gacccacggg aagatgactc tgcccctcat cctctattcg
      601 gctcgattgt catccgacaa ggaacgtcag gccctgtttg aactcctccg gaaccccaac
      661 ggccacctcc gcggattgat tgagcgagtc cgcgcgtccg gcgtcctcgc cgattgccgg
      721 acgaaagttc aacagttgtt gaacgaagcc gacggcgcgc tgaaggccct gccggacgtt
      781 ccggcgcggc gccttttggc ggacatcgcc cttcggctgt ccagccgaca agtttaacca
      841 cccccttttc aaagcggtat actgtgacaa accgccccgc gggcggtggg aggatccaat
      901 gttcggcatg ggttggcagg aattgctttt ggtgctcgtg atcatgttgt tgtttttcgg
      961 tcccaacaaa cttcccgcgc tgggcaaaag cctcggcaaa gccttggccg gcttcaagca
     1021 aggaatgaaa gagggcgcgg aggaagcgga caagggtctg cccgaggacc ccaaagccta
     1081 gcccttcgcc ctctccgacc gccgctttcc gtgaccgcgc ccgccgcgcc gccgatggac
     1141 cgcccgtccg acaccccggg ggaccgggcc cggcccctcg tcgatcacct caccgaaatt
     1201 caacggcgtt tacgctggag cctggccgcc gttctggcgt tgtccgtcgc cggctggttt
     1261 ttgacctccc cgattatcca ccgattggcc cggtccgtcg gaccgatgat ttacctggcc
     1321 cccaccgaag ctttcgccgt tcgctttaaa atggccgtgc tgttgggcgt gtttctgtcc
     1381 gcgcccgttt tgatttacca cctttggcgt ttcgtcggcg tcgccctgac tttgagcgag
     1441 cgccgggtcg tgttgggcgc cctcccgttc tcttacctcc tgttcgtggt cggcgcgtcc
     1501 ctgggctggt t
//
LOCUS       DAOQLS010000579         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_66753_length_1507_cov_3.094353, whole genome shotgun sequence.
ACCESSION   DAOQLS010000579 DAOQLS010000000
VERSION     DAOQLS010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66753_length_1507_cov_3.094353"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..>1507
                     /locus_tag="PK362_09640"
     CDS             206..>1507
                     /locus_tag="PK362_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75130.1"
                     /translation="MTADPDIKKYEALRDQCRAMAKDDPKGAYLNFQSSVMGFGAFPG
                     IDAVWSDACRTLADLMGPLAKGKIEDSLGRVRANPQDGQALYDLAYECFEVGQHGVAA
                     TLLAKAYRLSPGSAKIMTELISNLDEMGRHGDACRVLRESGLVEKDYFCRYLLAFHLV
                     MVGDLASAEKVPLVLSEAPENLRWPTEQVRHMLDRARAVKGIAPLDEWDLRGWHYVIN
                     GGLLLHLSPHGFKEAMRGRYAFVQDSYELCREGVDKVARLAKLWNLSGKVWSHPDRGS
                     EILGRAAGAALGWPLAPWPSKPTEEPGIFVVYDMERLNQDTFEVVENRAPGQLLWSHA
                     TKWTASAPFAADLVTFLHQSNQEPWGERLVVNPETKKAELAEPSQEDDEKLSIAILAA
                     KAEPETGDIPPDRIDYEPLARAILANAGKDFAGPLAPPAPRP"
BASE COUNT          286 a          482 c          483 g          256 t
ORIGIN      
        1 cctgccggcg gtggtgttgg acgccgcgcc gccggtcatc gacgcctccg cgcattttaa
       61 aacctgggaa gaccgtgtgc gggcggacgc cgagtccgtg caagccgccc gtcgtgtcag
      121 agaacccttg tttaagaggc tcttgcgcgc gttcctggat cggtgatgaa gcggatttaa
      181 acctggaccc tgtcaggacg aattcatgac cgccgacccg gacatcaaaa aatacgaagc
      241 ccttcgggac cagtgccggg ccatggccaa ggacgacccg aagggggcgt atttgaattt
      301 ccaatcctcg gtcatggggt ttggcgcctt ccccggaatt gacgcggtgt ggtccgacgc
      361 ctgccgaacc ttggcggacc tcatggggcc cctggccaag ggcaaaatag aggattccct
      421 gggacgggtg cgggccaacc cccaggacgg ccaggcgctc tacgacctgg cctatgaatg
      481 ttttgaggtg gggcaacacg gcgtggcggc cactctgctg gccaaggcct accgactgtc
      541 gccgggctcg gccaaaataa tgaccgagct tatcagcaat ttggacgaga tgggccgcca
      601 cggggacgcc tgccgggtgc ttcgggagtc gggcctggtg gaaaaggatt acttttgccg
      661 gtacctgctg gccttccacc tggtgatggt gggggacctg gcctcggcgg agaaggttcc
      721 cctggtctta agcgaagccc ccgaaaacct ccgctggccg accgagcaag tgcgccacat
      781 gctggaccgc gcccgggcgg tgaaaggcat cgcgcccttg gacgaatggg acctccgggg
      841 ctggcactac gtgatcaacg gcgggctact gctccatttg tcgccccacg ggttcaagga
      901 ggccatgcgc gggcgctacg cctttgtcca ggattcctat gagctttgcc gggaaggcgt
      961 cgacaaagtg gcccggctgg cgaagctgtg gaacctctcg ggaaaagttt ggagccaccc
     1021 ggaccgggga tcggaaatat tgggccgcgc cgccggggcg gccctgggct ggcccctggc
     1081 gccctggccc tccaagccga cggaagaacc aggtattttc gtggtttatg acatggagcg
     1141 gcttaaccag gacacttttg aagtcgtgga aaatcgcgcc cctggccagt tgctctggtc
     1201 ccatgccacc aaatggacgg cctcggcccc cttcgccgcg gaccttgtga ctttcctcca
     1261 ccaaagcaac caagaaccct ggggggaaag gctggtggtg aacccggaaa ccaaaaaagc
     1321 cgagttggcc gagccgagcc aagaggacga cgaaaagctg tccatcgcca tcctggccgc
     1381 caaggccgaa ccggagaccg gcgacatccc cccggaccgg atcgactatg agcccctggc
     1441 ccgcgccatc ctggccaacg ccggcaaaga ctttgccggc cccctagccc ctcccgcccc
     1501 ccgcccc
//
LOCUS       DAOQLS010000580         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_66874_length_1505_cov_5.240690, whole genome shotgun sequence.
ACCESSION   DAOQLS010000580 DAOQLS010000000
VERSION     DAOQLS010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66874_length_1505_cov_5.240690"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..657
                     /locus_tag="PK362_09645"
     CDS             55..657
                     /locus_tag="PK362_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNC75131.1"
                     /translation="MSGDVLVLNRQFYAIQITSLKRALTLLYLDHAHVVDQEYRTYSF
                     DSWRELSTGRAENPAGFIRTPSFNLAIPEVIALRVFDRLPLSQVKFTRRNIYEHYGYR
                     CCYCGVKFSTENLNLDHVVPRCREGATTWENIVTSCIPCNLKKGSRLPEEAGMKLLIH
                     PSRPKWKGPASLVFRSHFKVRQSWQRFVDNAYWNSELESK"
     gene            668..1030
                     /locus_tag="PK362_09650"
     CDS             668..1030
                     /locus_tag="PK362_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75132.1"
                     /translation="MNQSVLVVEDDPGIRDVLRLELSDQGFGVRTANSGEEALALLEK
                     SPVDLVLLDLRMPGMDGLETAQKIKSRWPALPIVLCTVCAEKRFERFIGNEIKAYLPK
                     PFTFHQLKETVSGALAAR"
     gene            1123..>1505
                     /locus_tag="PK362_09655"
     CDS             1123..>1505
                     /locus_tag="PK362_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNC75133.1"
                     /translation="MSDVPTSPSGSAPAPEGNFFNPEVTRRGLIAGAWGAFAAFLAGG
                     AAATARYMLPNVLYEPSQRFKMGKLKDLPQGVTVNKENRVWVIRDDKGVYALWSRCTH
                     LGCTPNWFQAESRFRCPCHGSNFNVA"
BASE COUNT          299 a          474 c          410 g          322 t
ORIGIN      
        1 ccttcccccg gcttttggcg gtcttgttgc aaggaagaaa acgggggccc gactatgtcc
       61 ggcgatgtat tggtccttaa tcggcagttt tacgccattc aaatcacctc gctcaagcga
      121 gcgctgaccc tcctttacct cgaccacgcc cacgttgttg accaggaata ccgcacttat
      181 tctttcgaca gttggcggga gctttccacc ggccgagccg agaaccccgc gggattcatt
      241 cgaacgccgt ccttcaacct cgccatcccc gaagtcatcg ccctgcgcgt cttcgaccgc
      301 cttccgctca gccaggtcaa attcacccgc cggaacatct acgaacacta cggctaccgc
      361 tgttgttatt gcggggtcaa attttccacc gaaaacttga atttggatca cgtggttccc
      421 cggtgtcggg agggcgcgac cacctgggaa aacatcgtga cgtcctgcat cccctgcaat
      481 ttgaaaaaag ggagccgcct gccggaagag gccggcatga agcttttgat ccacccctcc
      541 cgacccaaat ggaaagggcc cgcgtccctg gtctttcgat cccactttaa agttcggcag
      601 tcctggcagc ggttcgtcga caacgcgtat tggaactcgg aacttgaatc caaatagggg
      661 gtgtgcgttg aatcaatcgg ttctcgtggt ggaggacgat cccggcatcc gggacgttct
      721 tcgtttggag ttgtcggacc aggggttcgg tgttcgcacg gccaattccg gcgaggaagc
      781 cctcgccctt ttggaaaaat cgccggtgga tttggtgctg ctggatcttc gcatgcccgg
      841 catggacgga ttggaaacgg ctcagaaaat caaaagccgc tggccggccc tgcccatcgt
      901 cctctgcacg gtgtgcgcgg aaaaacgctt tgagcgtttc atcgggaacg aaatcaaagc
      961 ctatttgccc aagcccttca cttttcacca actgaaggaa acggtgagcg gggccctggc
     1021 ggcgcgctga aaccgcgagc gccggtccct cggtttgaca ccgatcgggt gatcttgtaa
     1081 actcaggccg caacgccaat ttcatcaaac gagtcttcgt ccatgtccga cgttcccaca
     1141 tccccgtcgg gttccgcccc ggcgcccgaa ggaaactttt tcaaccccga agtcacccgt
     1201 cgcggtttga tcgccggcgc ctggggcgcc tttgcggcct ttctcgccgg cggcgccgcc
     1261 gccaccgccc gctacatgct ccccaacgtt ctctacgaac cgtcccagcg gtttaaaatg
     1321 ggaaaattga aagacctgcc ccagggcgtg acggtgaata aagaaaaccg cgtctgggtc
     1381 atccgggacg acaaaggggt ttacgcgctc tggtcccggt gcacccactt ggggtgcacg
     1441 cccaattggt tccaagccga aagccgcttt cgttgcccct gccacgggtc caatttcaac
     1501 gtggc
//
LOCUS       DAOQLS010000581         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_66972_length_1504_cov_3.920635, whole genome shotgun sequence.
ACCESSION   DAOQLS010000581 DAOQLS010000000
VERSION     DAOQLS010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66972_length_1504_cov_3.920635"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PK362_09660"
     CDS             <1..620
                     /locus_tag="PK362_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75134.1"
                     /translation="LQFRVDSNRLPTYNPDGDPDGDGVPNLKDPDDDNDLGTLSPPAQ
                     AWRYGDDLRDDDDDNDGKIDLQVRYVLANGTLTRDQNFNEGGWGQHVEVVAKDLLTAQ
                     SSFTYFGSKNQALGKKIDLGNDGLPGTADPGENDNVIVSTEIDRVLPPTGGGSANGAL
                     DTDLERQYIVSLHVVLRLDANHDGKEDGRFETDYEPPLLPLKRSF"
     gene            621..>1504
                     /locus_tag="PK362_09665"
     CDS             621..>1504
                     /locus_tag="PK362_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75135.1"
                     /translation="MKPSPPRGSAFIQVLILLVFVVPLILFFLKSAARQNKSALQERQ
                     IKESRALTTNVMVDFMRQFSQNQTASYLDTSALARPETFSGAGFSDVTTEINALGHFI
                     YFRSTGRFGDPGGAVKHLDAVIRFAADLTRFGNLFNNTITISANGPTYNGGFYVNGNL
                     TVTGANATFNSGPVVVKGNLTVPASAVFNSDVYVEGATAFNGAAVNGVIYRYVPNIDW
                     PTIDTTYYSLHNNYAVDGTSQTWVFSVQTSTQGIFTVQGTTVVVKIPSTGGVFFANNT
                     NLTVRGTVRGRVTVVVAGP"
BASE COUNT          328 a          474 c          427 g          275 t
ORIGIN      
        1 cccttcagtt ccgggtggac agcaatcgcc tgccaacgta caacccggac ggcgaccccg
       61 acggcgacgg cgtgccgaac ctcaaagacc ccgacgacga caacgatctg ggcaccctga
      121 gccccccggc ccaggcttgg cgttacggcg acgatttgcg ggacgatgac gacgacaacg
      181 acgggaagat cgacctccag gtccgttacg tcctggccaa cgggacgctg acccgcgacc
      241 aaaatttcaa cgaaggcggc tggggccaac acgtggaagt ggtggcgaag gatctcctga
      301 cggcccaaag ttcttttacg tatttcggtt caaaaaacca agccctgggc aagaaaatcg
      361 atttgggtaa cgacggattg ccgggcacgg ccgaccccgg cgaaaacgac aacgtcatcg
      421 tttccacgga aatcgaccgg gttttgccgc cgacgggcgg cgggagcgcc aacggcgctc
      481 tggacacgga cctggaaagg caatacatcg tgtccctcca cgtggtcctt cgcctggacg
      541 ccaaccacga cgggaaggaa gacgggcgat ttgaaacgga ctacgaaccg cccctcctgc
      601 ccttgaaaag gtccttttaa atgaaaccgt cgccgccccg gggatccgct ttcatccaag
      661 tgctgatcct cctggtcttt gtcgttcccc tcatcctctt tttcctcaaa agcgccgccc
      721 gccagaacaa atcggccctt caggaaagac aaatcaagga atcccgcgcg ttgaccacca
      781 acgtcatggt cgatttcatg cggcagtttt cccaaaacca aacggcgtcc tatttggaca
      841 cgtcggcttt ggcccgaccc gaaacgtttt ccggcgcggg tttttcggac gtcaccaccg
      901 agatcaacgc cctgggccat ttcatctatt ttcggtccac gggacgcttc ggcgacccgg
      961 gcggagccgt caagcatttg gacgcggtca tccgattcgc ggccgacctg acgcgcttcg
     1021 gcaatctgtt caacaacacg atcacgattt ccgccaacgg acccacctac aacggcggtt
     1081 tttacgtaaa cgggaacctg acggtcacgg gcgccaacgc gaccttcaac agcggacccg
     1141 tggtggtcaa aggcaatctc acggtgccgg ccagcgcggt gttcaattcc gacgtctacg
     1201 tggagggggc gacggccttc aacggggcgg ccgtcaacgg cgtgatttac cgttacgttc
     1261 cgaacatcga ctggcccacc atcgacacga cttattacag ccttcacaac aactacgcgg
     1321 tggacggaac gtcccagacc tgggtcttca gcgtccaaac gagcacccag gggattttca
     1381 cggttcaagg gacgacggtg gtggtgaaaa ttccttcgac gggaggcgtg ttttttgcca
     1441 acaacacaaa tctcacggtt cggggaacgg tgcgggggcg ggtgaccgtc gtcgtggcgg
     1501 gccc
//
LOCUS       DAOQLS010000582         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_66976_length_1504_cov_3.786749, whole genome shotgun sequence.
ACCESSION   DAOQLS010000582 DAOQLS010000000
VERSION     DAOQLS010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66976_length_1504_cov_3.786749"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK362_09670"
     CDS             <1..213
                     /locus_tag="PK362_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HNC75136.1"
                     /translation="GAPVETVNLGGVHDGPGRSAVTPHLFLTDADKNDLRALAARGLR
                     IEARALPTDAAADWKDLDIAGPVAGR"
     gene            210..878
                     /locus_tag="PK362_09675"
     CDS             210..878
                     /locus_tag="PK362_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HNC75137.1"
                     /translation="MIPFNPLGVGLLYAVLSLDHFSFGPLLLSRPLVVGTACGWALKQ
                     PAAGFEIGAVGEALWALVPPAGPRQWDVGLMAALATAWLAGDSAPAGAPRWALALAGL
                     IAFPLGLLGRRIDGWARKQTRPFAAHALEGLSRGSDGPLRASLFATALLWVFKSFLVF
                     LVMESFGGALWDWIASRTPALFQTALERTWVLWIALGAGALLWQFIGRWPARSLRPGP
                     AAQP"
     gene            875..>1504
                     /locus_tag="PK362_09680"
     CDS             875..>1504
                     /locus_tag="PK362_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HNC75138.1"
                     /translation="MNRGVLARVFLRSLWLQGLWTVQRGQNLGCLYSLWPVVDGLYDR
                     PEDRAEVARTHAGFFSTHPYTAGVVLGVVAGLETDRARGNGPSGEAIAAARSAMAGPL
                     AALGEAFFWSLWRPLSFLAAMALAWAHPGHPGRIVLSFLVIYNLLHLGARWQGLRWGF
                     RWRTDVVPRLAPLRLQRVLTLAAAAGALATLVLMIAAVATGRASPGLLGA"
BASE COUNT          160 a          492 c          542 g          310 t
ORIGIN      
        1 ggcgcccccg ttgagacggt gaatttgggc ggcgttcacg acggaccggg gcggagcgcc
       61 gtgacgcccc atctcttttt aacggacgcg gacaaaaacg atcttcgcgc cctcgcggcc
      121 cggggccttc ggatcgaagc ccgcgcgctg ccgacggacg cggcggccga ttggaaggac
      181 ttggacatcg ccggccccgt ggcgggacgt tgattccgtt caatcccctc ggcgtcggat
      241 tgctatacgc cgtcctgtcg ttggaccatt tttccttcgg accccttttg ctgtcgcggc
      301 ccctggtcgt gggcacggcc tgcggttggg cgttgaagca accggcggcg ggatttgaaa
      361 tcggcgccgt gggcgaggcc ttgtgggcgt tggtgccccc ggccggtccg cggcaatggg
      421 acgtcggttt gatggcggct ttggccacgg cctggttggc gggcgatagc gccccggccg
      481 gcgccccacg ctgggccctg gccctggcgg gattgatcgc ttttcccctg ggtcttctgg
      541 ggcgtcgcat cgacggttgg gcgcgtaaac aaacccggcc cttcgcggcg cacgcgctcg
      601 agggtctttc gcgtgggtcc gacggaccgt tgcgggcgtc cttgttcgcc acggccctcc
      661 tttgggtttt taaaagcttt ctcgtttttc tggtaatgga gtccttcggg ggcgccctct
      721 gggattggat cgcgtcacga acgccggccc tgtttcaaac ggccctggag cgcacgtggg
      781 tcctttggat cgccctgggg gccggggcgc ttctgtggca attcatcggg cggtggcccg
      841 cccgatccct ccggccggga ccggcggccc aaccatgaac cgcggggtgc tcgcccgggt
      901 cttcctccgc tcgctgtggc tgcaggggct ctggaccgtt cagcggggtc aaaatctggg
      961 ttgtctctac agtttgtggc cggtcgtgga tgggctctac gatcggccgg aggatcgggc
     1021 ggaagtggcc cggacgcacg cggggttttt cagcacccat ccgtacaccg ccggggtcgt
     1081 ccttggcgtc gtggccggtt tggaaacgga ccgtgcccgg gggaacggtc cgtcgggcga
     1141 agccatcgcc gcggcccggt cggccatggc cggccccctg gcggccctgg gagaagcgtt
     1201 tttctggtcc ttgtggcggc ccctgagttt tctcgcggcg atggccctgg cttgggccca
     1261 tcccgggcac ccggggcgga tcgtcctttc gtttttggtg atttacaacc ttctgcacct
     1321 gggcgcccga tggcaagggc ttcggtgggg gtttcgctgg cggacggacg tggtgccgcg
     1381 cctggccccg ctccggcttc aacgcgttct gaccctcgcg gcggccgccg gcgccctggc
     1441 caccctcgtt ttgatgatcg ccgccgtggc cacgggccgg gcgtccccgg ggctcctcgg
     1501 cgcg
//
LOCUS       DAOQLS010000583         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_67814_length_1495_cov_3.190278, whole genome shotgun sequence.
ACCESSION   DAOQLS010000583 DAOQLS010000000
VERSION     DAOQLS010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67814_length_1495_cov_3.190278"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PK362_09685"
     CDS             complement(<1..801)
                     /locus_tag="PK362_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC75139.1"
                     /translation="MPTWATNTHDTLMPIASLEDLAKRLRVSRRTVSRVLTGGKNVAP
                     AVRERIEKYLARTDYVPNAQAARLAARRVPVLGLVFPTNFLNTLDDYVVRIIKGALRA
                     AHASGHQVTLLAFDSLDVKQTLKLHRGRLVGEFVFTAFGPPDAKALRHLRSEGVPLAA
                     VNFCAEGVDSYDCDNVRGGYLATKHLLDQGRRRIAFLHGSRWWVSSRDRHLGYTQALA
                     EAKIELPESFVVEANYEPVAAEKTTDKWLESPKGPDAIFAANDIMMLAS"
     gene            complement(843..>1495)
                     /locus_tag="PK362_09690"
     CDS             complement(843..>1495)
                     /locus_tag="PK362_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC75140.1"
                     /translation="ESPCYRCLYPEPPPPGEVPSCAEGGVLGILPGLVGLIQATEAIK
                     LILGKGRSLVGRLLLFNALEMTFDEVKIRRNPKCPVCGDKPTIKELIDYDQFCGIGRG
                     NEGAAAANGSAEITVKQLADRLNRKDKFVLIDVREPNEWDIARIPGAKLLPLSEIAER
                     ANELDTADDIVVHCKSGVRSQKAIGVLKQLGFRRLTNVKGGILAWSDEIDPAVPKY"
BASE COUNT          262 a          433 c          508 g          292 t
ORIGIN      
        1 ggaggcgagc atcatgatgt cgttggcggc gaaaatggcg tcggggccct tgggggattc
       61 cagccacttg tccgtcgttt tctcggccgc gaccggttcg tagttggcct ccaccacgaa
      121 ggattcgggc aattcgattt tcgcttcggc cagggcctgg gtgtagccca ggtgccggtc
      181 ccgggaggag acccaccaac gggaaccgtg caggaaggcg atgcggcgac ggccctggtc
      241 gagcaagtgc ttcgtggcca aatagccgcc ccggacgttg tcgcaatcgt aggagtccac
      301 gccctcggcg cagaaattga cggcggccag gggaacccct tccgagcgca gatggcggag
      361 agccttggcg tccggcgggc cgaaggcggt gaagacgaat tcccccacca gccgtcctcg
      421 gtgaagcttc agggtttgtt tgacgtccag gctgtcaaag gccaggaggg tcacctgatg
      481 tcccgaggcg tgggcggccc gaagggcgcc cttgatgatc cgaaccacgt aatcgtcgag
      541 ggtgttcagg aagttggtcg ggaacacaag acccaaaacg ggtacccgtc gggcggccag
      601 ccgagcggcc tgggcgttgg gaacgtaatc ggtgcgggcg aggtatttct cgatgcgctc
      661 ccggaccgcc ggggcgacgt tttttcctcc ggtgagaacc cgggaaaccg tccgccggga
      721 aacccgaagg cgtttggcca aatcttcaag ggaggcgatg ggcatgagag tgtcgtgggt
      781 gttggttgcc cacgtgggca tatcctatat aatttatttg tcgggcgcca gggtcgggcg
      841 cgtcagtatt tcggtacggc cgggtcgatt tcgtccgacc aggccaaaat gccgcctttg
      901 acgttggtca gccggcgaaa acccaactgc ttcaacacgc cgatggcttt ttggctccgg
      961 actcccgact tgcaatgaac gacgatgtcg tcggcggtgt cgagttcgtt ggcccgctcg
     1021 gcgatttcgc tcaaaggcag gagcttcgcg ccgggaatgc gggcgatgtc ccactcgttg
     1081 ggttcgcgaa cgtcgatcaa gacgaacttg tccttccggt tcaaccggtc ggccaattgt
     1141 ttgacggtga tttccgccga tccgttggcc gcggccgcgc cttcgtttcc ccggccgatg
     1201 ccgcagaact gatcgtagtc gatcaactcc ttgatcgtgg gcttgtcccc gcagacgggg
     1261 catttgggat tccgccggat tttgacttcg tcgaaggtca tctccagggc gttgaagagc
     1321 aggaggcggc ccacgaggga ccggcctttc cccaaaatca atttgatggc ctccgtggcc
     1381 tggatgagac cgaccaggcc gggaaggatg cccaacacgc ccccttcggc gcaggagggc
     1441 acttcgcccg gcggcggcgg ttccgggtag agacagcggt agcaggggct ctcgg
//
LOCUS       DAOQLS010000584         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_68568_length_1487_cov_3.301676, whole genome shotgun sequence.
ACCESSION   DAOQLS010000584 DAOQLS010000000
VERSION     DAOQLS010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68568_length_1487_cov_3.301676"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PK362_09695"
     CDS             complement(<1..1011)
                     /locus_tag="PK362_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC75141.1"
                     /translation="MAGTTARSANRLAEGREELERIQSKLSDKKREKEKADKRSRELR
                     AEVDRISRELTGARRALAQTNARLAESEEKRSAAEARLWASRQSLTQWQERLASALGA
                     YSRRSRTGSEGDFVSVFYERALVADQASSLGYALERHQDVVVLRDNLLAIEDRLRDLK
                     LDRERDERRIEAAAERMRELQKTTEGRRAVLESDIRALNASAQKFERLILDLIAKQKA
                     EDARRAARAKRAAPVPVAQEEISRRWRGRLPRPVEGTVVEKFGRTRNAEVNADVFSNG
                     VKWRTAPDANVAAVAEGEVLYAGPFMNYGLMVLVGHPDGLHSIYAHLSQTPMVRGQKI
                     AAG"
     gene            complement(1065..>1487)
                     /locus_tag="PK362_09700"
     CDS             complement(1065..>1487)
                     /locus_tag="PK362_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75142.1"
                     /translation="NPFPESFDAVWNPDFLRPDYLSPLSEQVAGWDGVDRVAYDGPRL
                     DRIALLSRLKVQRRAGVSILAGGTLAALVFLAGRWLFFSRRWRLAEILLAAGVGALGG
                     SAGAAAAAAWLGAFDLPGTIAGLALGLLTGLGRAAADE"
BASE COUNT          259 a          555 c          450 g          223 t
ORIGIN      
        1 cccggcggcg attttttggc cgcgaaccat cggggtttga ctcaaatggg cgtaaatcga
       61 atgcagtccg tccgggtggc cgaccagcac catcaggccg taattcatga aggggcccgc
      121 gtagagaacc tcgccctcgg ccaccgcggc cacgttcgcg tcgggggcgg tccgccattt
      181 gaccccgttt gaaaacacgt cggcgttgac ttcggcgttc cgcgtccgtc cgaatttttc
      241 cacgacggtc ccctccacgg gacggggcag ccggccgcgc catcgccggg agatctcctc
      301 ctgggcgacg ggaaccggcg ccgcgcgctt cgcccgggcg gcccggcgag cgtcttccgc
      361 cttctgcttg gcgatcaaat ccaggatcag ccgctcaaac ttctgggccg aggcgttcag
      421 cgcgcggatg tcgctttcaa gcacggcccg gcgcccctcg gtggtcttct gaagctcgcg
      481 catgcgctcg gccgccgcct cgatgcggcg ctcgtcccgc tcgcggtcca acttcaaatc
      541 ccgcaaacgg tcctcgatcg ccagcaaatt gtcccgaagg accaccacgt cctggtggcg
      601 ttccagggcg tatcccaggc tcgacgcctg gtccgccacc aacgcccgct cgtagaacac
      661 cgacacgaaa tcgccttccg acccggtgcg ggaccgtcgg gagtaggcgc ccagggccga
      721 cgccagccgt tcctgccact gggtcaaact ttgtcgggag gcccacaaac gcgcctccgc
      781 ggcggagcgc ttctcctcgg attcggccaa ccgcgcgttg gtttgcgcca gcgcccgccg
      841 ggcgccggtc aattcccggg aaatccggtc cacttcggcg cgcaactcgc gggaccgctt
      901 gtccgctttt tccttttcgc gtttcttatc ggacagcttg gactggatcc gctccaattc
      961 ctcgcggccc tcggccaatc ggttggccga ccgggccgtt gttccggcca ccgcgaccag
     1021 cgccgccgcc ccgagcggaa aaaaccacgt cgataaaaag cgcctcactc gtcggcggcc
     1081 gcccgaccca aacccgtcag aaggcccaaa gccaacccgg cgatcgtccc cggaaggtca
     1141 aacgcgccca accacgccgc ggcggcggcc gctcccgcgg acccgcccag agcgccgacg
     1201 cccgcggcca acaaaatctc ggccaaccgc caacgccggg aaaagaacag ccaacgtccc
     1261 gccaggaaaa cgagggccgc gagggtcccc ccggccagaa tggaaacccc ggcgcgccgc
     1321 tgaaccttta accgggacaa caacgcgatc cggtcgaggc ggggcccgtc gtaggccacg
     1381 cggtccacgc cgtcccaccc ggccacctgc tccgagagcg gcgacagata atccggacgc
     1441 aagaaatccg ggttccacac ggcgtcgaag gattcgggaa agggatt
//
LOCUS       DAOQLS010000585         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_68942_length_1483_cov_4.287815, whole genome shotgun sequence.
ACCESSION   DAOQLS010000585 DAOQLS010000000
VERSION     DAOQLS010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68942_length_1483_cov_4.287815"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PK362_09705"
     CDS             <1..1048
                     /locus_tag="PK362_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HNC75143.1"
                     /translation="FPEESPFLDSPDGASASVVVTLQPGSTLEKDQIRGIVHLVSGAV
                     KGLKPENVSIVDVQGRLLAGGRAADSVFSQGNDLLELQKEAEHYLEGKASSLLDGLLG
                     AGRSLVRIRVDMDAQIIKEQTERYDPTGPVRSEQTVAGGGTGPSTVKNYEVGRTMKEI
                     VASPGAVTRMYISLAVDGTFADDPAKPGERVYKPRTEDELRTIAQLVKEAVGFSEDRE
                     DQLEIRNVPFDTSRNDESAKERRQNEEAMRRDRQRAQYLEVAKHLGTSLGMGLIVLAL
                     LRLVKGDSRPVPAEVPAPVAPAPPPLPVEVPPVRAEKPFNHAEAEALILKTARQNPAD
                     LAKLMQRQFLTNAS"
     gene            1050..>1483
                     /locus_tag="PK362_09710"
     CDS             1050..>1483
                     /locus_tag="PK362_09710"
                     /inference="COORDINATES: protein motif:HMM:NF026192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75144.1"
                     /translation="MPDKNLPAVPVLTPFQKVSVLLRSLPDDQCVEVLKNYPEADVEQ
                     ICREMMNPPAVPEETRVQLLKEFNEQLGAATARPPAENIEGILARLYGVRRSGEILRR
                     MKESAVRPPSLTGLIDAVGLAAAAEEMGRELPAVAAFALGELP"
BASE COUNT          294 a          476 c          480 g          233 t
ORIGIN      
        1 gtttcccgaa gagtcgccct tcctggattc gcccgacggg gccagcgcct cggtcgtcgt
       61 gacgctgcaa ccgggatcga cgctggaaaa agaccaaatc cggggcattg tccatttggt
      121 gtcgggcgcg gtcaaagggc ttaaacccga gaacgtatcg atcgtggacg tccagggtcg
      181 cttgctggcc gggggccggg ccgccgacag cgtgttctcc cagggcaacg atctgttgga
      241 acttcaaaaa gaagcggaac attatctgga gggcaaggcc tcgtcgcttc tggacgggct
      301 tctgggcgcg ggacgttccc tggtccgcat ccgggtcgac atggacgcgc aaatcatcaa
      361 ggaacaaacg gagcgttacg acccgacggg ccccgtccgc agcgagcaga cggtggccgg
      421 gggcgggacc ggcccctcga ccgtcaaaaa ctacgaggtg ggccgcacca tgaaagaaat
      481 cgtcgccagc cccggggccg tcacgcgaat gtacatcagc ttggcggtcg acggcacgtt
      541 cgccgacgat ccggccaagc ccggcgagcg cgtctacaag ccccgtaccg aagacgaact
      601 gcgaaccatc gcccaattgg tcaaggaagc cgtggggttc agcgaagacc gggaagatca
      661 attggaaatc cgcaacgtgc ctttcgacac cagccggaac gacgagtcgg cgaaggagcg
      721 ccgccaaaac gaggaggcga tgcgccggga ccgtcaacgg gcccaatacc tggaggtggc
      781 caagcacttg ggcacctccc tgggcatggg gttgatcgtt ctcgccctgc ttcgccttgt
      841 gaagggggat tcgcggccgg tccccgcgga agtgcccgcg cccgtcgcgc ccgcgccgcc
      901 gccgctgccc gtggaagtcc cgccggtgcg tgcggagaaa ccgttcaatc acgcggaggc
      961 cgaagccctc attcttaaaa ccgcccggca aaacccggcc gatttggcca agctgatgca
     1021 gcgccaattt ttgacaaacg cgtcctaacg tgcccgataa aaaccttccc gcggttccgg
     1081 tcctgacccc ttttcaaaag gtgtcggtgc tgttgcggtc cctgccggac gaccaatgcg
     1141 tcgaagtcct caagaattac ccggaggccg acgtcgagca aatatgccgg gaaatgatga
     1201 acccgccggc cgtgccggaa gaaacccgcg tgcagttgct caaggaattc aacgagcaac
     1261 tgggggccgc caccgcccgc ccccccgccg aaaacatcga gggaatcctg gcgcgtcttt
     1321 acggggtgcg acggtcgggg gaaattctgc gccggatgaa agaatccgcc gttcgcccgc
     1381 cgtccctgac gggtttgatc gacgccgtgg gcctcgccgc ggcggccgag gaaatgggcc
     1441 gggagctccc ggccgtggcc gctttcgccc tgggggaact tcc
//
LOCUS       DAOQLS010000586         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_69118_length_1481_cov_4.450912, whole genome shotgun sequence.
ACCESSION   DAOQLS010000586 DAOQLS010000000
VERSION     DAOQLS010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69118_length_1481_cov_4.450912"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..255)
                     /locus_tag="PK362_09715"
                     /pseudo
     CDS             complement(61..255)
                     /locus_tag="PK362_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
     gene            complement(1236..1481)
                     /locus_tag="PK362_09720"
                     /pseudo
     CDS             complement(1236..1481)
                     /locus_tag="PK362_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
BASE COUNT          237 a          440 c          488 g          316 t
ORIGIN      
        1 ctgccgacgc tatcgcgaat atcggatagg tttcatcgag gaacgaacgc atggacatca
       61 ccggcaggcg ccgccggtga tgtccctgcg ttcgggggcg tcggtgttga aggtgtaggt
      121 cccggggtag atgatccccg ccgttttctt cgagccgtct ttaaaaagga tcgtgtggct
      181 gacgactttt ccgtcgaaat agacgttcgc cttgggtttg acgctcacgt tgtcgaattg
      241 ctccggtacg ctcatggtca ccttttcttg aagagggatt tgattttttt aagatcggag
      301 gacccgtcgg cgcccgcggt cccgcggagg gcgttttcca gacggtcccg aagggcgtcc
      361 agggcctttt cctggccgcc cagcttttcc ccaatgggct ttagccgccc gtccaggagg
      421 cccgccaatt gggatcggtc gccgtccacc aggccgttca cccgggattt tagttttcct
      481 tccagggcgt ccatttcgct tttggcggcg tttcttagga tgccgttcag ctcgttgtcc
      541 agggtggagg agattttgat gcggggccga ggggcggttc cggagacctc aaaagtcacc
      601 cgggtttggg ttactcccgc cagggcgttt ttgagcgccc gggccacggc gcccgtgtcc
      661 gacgactcgc cgattttaag acgcaggccc gagaggtcca agtcgatggt cccgtcgatg
      721 ttgtccccgg cgctttgaag tcgagcggtc aggcggccca gaccttggtc caccgtgacg
      781 ggcccaccga catcgcccag ggcggtgccg gccagcgaaa ggccgttcag gatcagggcg
      841 gccgtgcccc gggcgggagc gcgcgccagg tcgagggagg cgtccaattg gagcgactga
      901 gcggcggatt tcccttccag gttgaacgtg atcgggcggc ccagaccttc ggggtccgag
      961 gaaacgtccc gaagggtgcc acggtattgg atcccctcgg ccgtctcgat tttggccaga
     1021 tcccggtccg aaacctgaag ggttgacccg ctgacctcgg cgatttttaa gagaaaggcg
     1081 ggccagcggt aatggaaagg aaaagcgatg tcttgacccc ggggcgcggg ggcgggggcg
     1141 gccgccgtct tggatttctt cccgtcggcg ccgcgaagtt tttgaatctt atggaaccat
     1201 cccagagccg tttggatttt gcccatccaa acgggtccca ccagtgcccg ggtcacgccg
     1261 tcggcggaaa acccggagcg gaccagggcc aggcgtcggt cgatgtcctc tttttttagc
     1321 cgttcgatgt cggccaggtc gctcaggccg cccgaagcga tcaccggcac cgtcagcgcc
     1381 tgcgcgagct tgacggtcgc ctcgatgttg attccggaga gcatgccgtc gcggccgatg
     1441 tcggtgtaga tcacgccctc gacgccgtag tcctcgaact t
//
LOCUS       DAOQLS010000587         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_69508_length_1477_cov_3.922644, whole genome shotgun sequence.
ACCESSION   DAOQLS010000587 DAOQLS010000000
VERSION     DAOQLS010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69508_length_1477_cov_3.922644"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK362_09725"
     CDS             <1..266
                     /locus_tag="PK362_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75145.1"
                     /translation="KVYLTTGSEEIWDGVVYPRLAGNLGSLRGDPGTRFYVILKRDWW
                     ERLTAWLKWEVTHRPAAEEGRTGSAAAPRQAWHLEVSGRWGEK"
     gene            280..>1477
                     /locus_tag="PK362_09730"
     CDS             280..>1477
                     /locus_tag="PK362_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC75146.1"
                     /translation="MIYDVAVVGGGITGAGVVRDAALRGLSAVLIEKNSPGAATTASS
                     THLIHGGLRYLLYDRLTTHTTCWDSGHIVRIARPLLTRIPILWPVYDYHRHGIETVET
                     LLESYDGFQQMKEGRPHLRLTAEETRRLVPRLRPQGLRGGLLFDEWWVDPIALVRANL
                     DSALRAGAQIRTGQVVTGFLRDGARVRGVRVRLGGESQDLSARIVVNAAGPWVDRVAR
                     SAGVEVPLRLRKGVHLIYRNNVGLSPERLGLLLEAEDRERYVFVIPGADGSTLVGPTD
                     TPTPEGPDALTASAEDKRYLLASVRAYVPDFPESFDGVSVGARPILGQAGSEKYLSRE
                     FEIIDHGARDGVPGLVTAAGGKMSDFRLMAEAAVDTTARLLGMSALSRTAHETLAGEP
                     LDGALDR"
BASE COUNT          225 a          492 c          505 g          255 t
ORIGIN      
        1 ccaaagtgta tttgaccacg ggttccgagg aaatttggga cggcgtcgtt taccctcgtc
       61 tggcgggaaa cttgggatcc cttcgcggtg acccgggaac gcggttttac gtcattctca
      121 agcgggattg gtgggaacgc ctgacggctt ggttgaaatg ggaggtgacc caccgccccg
      181 ccgccgaaga ggggcggacc gggagcgcgg cggcgccccg gcaggcctgg cacctggaag
      241 tctccggccg ctggggggag aagtgataca atgcccccga tgatttacga cgtggccgtg
      301 gtggggggcg ggatcaccgg cgcgggcgtg gtccgggacg cggcgctccg gggcctctcc
      361 gccgttctga tcgaaaagaa cagtcccggc gccgccacca ccgcctcctc cacccatttg
      421 atccacggcg gcctccgcta cctcctttac gaccgcttga ccacccacac cacctgctgg
      481 gactcgggcc acatcgtccg aattgcccgg ccccttttga cccgcattcc catcctttgg
      541 cccgtctacg attaccaccg ccacggcatc gaaacggtgg aaaccctgct ggaaagctac
      601 gacggattcc aacaaatgaa ggagggccgt ccccacctac gcctcacggc cgaggaaacc
      661 cgccgcctgg tgccccgcct gcggccccag ggccttcggg gcggccttct tttcgacgag
      721 tggtgggtgg accccatcgc cctcgtccgg gccaatttgg attcggccct gcgggccggg
      781 gcccagatcc gcacgggcca ggtggtgacg ggctttttga gggacggggc ccgggttcgg
      841 ggcgtgcgcg tccgactggg cggtgagtcc caggatcttt cggcccggat cgtggtcaac
      901 gccgccggcc cctgggtgga ccgggtggcc cgaagcgcgg gggtggaggt gccgcttcgt
      961 ctgcgcaagg gcgttcattt gatttaccgc aacaacgtgg gattgtcccc ggaacggctg
     1021 ggcctgttgt tggaagccga ggaccgggag cgctacgtgt tcgtgattcc gggcgccgac
     1081 gggtccacgt tggtgggccc cacggacacc ccgacccccg aggggcccga cgccctcacg
     1141 gcctcggcgg aggacaagcg ctatttgttg gcttcggtgc gcgcctacgt tccggatttt
     1201 cccgaatcct tcgacggcgt cagcgtcggg gcccgtccca tcctggggca ggccgggtcc
     1261 gaaaaatatt tgagccggga atttgaaatc atcgaccacg gggcccggga cggcgtgccg
     1321 gggttggtga ccgccgcggg ggggaaaatg tccgatttcc ggctcatggc cgaagccgct
     1381 gtggacacca ccgcccgcct tctgggcatg agcgccctgt cgcgcaccgc ccacgaaacc
     1441 ctggccggcg aaccgctgga cggtgccctc gaccggc
//
LOCUS       DAOQLS010000588         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_69520_length_1477_cov_3.324895, whole genome shotgun sequence.
ACCESSION   DAOQLS010000588 DAOQLS010000000
VERSION     DAOQLS010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69520_length_1477_cov_3.324895"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1477)
                     /locus_tag="PK362_09735"
     CDS             complement(<1..>1477)
                     /locus_tag="PK362_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75147.1"
                     /translation="VFNIGPSQGTVNTLQDPDLQKPVLKFDFTAPAGALVGVWTKDYP
                     STVNAGAVQRVVVSVKGPTVPPPASVALEIKGVREVQRLPLVLRAGWNRTESAIDWGR
                     IGALKEIVFVVSSAPGQKAAGTLLFDLEFAAGAPASAAVLPADAQALDAAHAPARGFF
                     NIGPADGKATAVPGPDGRGALQFDYAIPPKSMAGVWTKGYPPALSPATANAVEATVRF
                     PAAGAARRLSATVELKGTKAVQRIPLELTDGVSKSLHAVDWKTIGKLKEAVLVVSPAG
                     GKSETGTLLFNLAFVKRAVLPKTGGARRLAEVAERGAFNIGKAQGTISRVRDGELRRD
                     VLRFDFTAAPKAVVGLWSKGYAADLSAAVANAVSVGVKGGRGAPVAAVGLEIKGDKET
                     QRIPLSVGAEWTRTDRAVEWDAIGKVKEVVFVVTPLDPAKKSAGRFYFDLAFEKTPVA
                     PAGAPGLGLLSATEKGVFTIGGAEGRVVIESEAAAKKNVYKF"
BASE COUNT          258 a          499 c          461 g          259 t
ORIGIN      
        1 gaatttataa acattttttt tggccgcggc ctcggactcg atgaccacgc gcccttccgc
       61 gccgccgatg gtgaacacgc ctttttccgt ggcgctcaag agacccaacc ccggcgcgcc
      121 ggcgggcgcc accggtgttt tttcaaacgc cagatcgaaa tagaaacggc ccgcgctttt
      181 tttggccggg tccaggggcg tgacgacgaa aacgacttcc tttacctttc cgatcgcgtc
      241 ccactccacc gcccgatcgg ttcgggtcca ttcggcgccc acggaaaggg gaatgcgttg
      301 ggtttctttg tcgcccttga tttccaaacc caccgcggcc acgggggccc cgcgcccgcc
      361 tttgaccccc acggacaccg cgttggccac ggccgcggac aaatccgccg cgtagccctt
      421 gctccacaac cccacgaccg ccttgggcgc ggccgtgaaa tcaaaccgaa ggacgtcccg
      481 gcggagttcc ccgtcccgaa cgcgggaaat cgttccctgg gctttgccga tgttgaatgc
      541 gccccgctcc gccacctccg ccaaacggcg ggcgccgccc gttttgggca acacggcgcg
      601 cttcacgaac gccaaattaa agagaagggt tcccgtttcg cttttccccc cggccggcga
      661 cacgaccagg acggcctctt tgagctttcc gatggttttc caatcgacgg cgtgaaggga
      721 tttggataca ccgtcggtca actcgagggg gatgcgttgg acggctttcg ttcctttgag
      781 ttccaccgtg gccgacaaac ggcgcgcggc gcccgccgcg ggaaaacgca ccgtcgcctc
      841 caccgcgttc gccgtggccg gggaaagggc gggcggatag cctttcgtcc acaccccggc
      901 catggatttg ggcgggatgg cgtaatcgaa ttggagcgcg ccccggccgt cggggcccgg
      961 aacggcggtc gctttcccgt cggcgggtcc gatgttgaag aacccccggg cgggggcgtg
     1021 ggccgcgtcc agggcctggg cgtccgcggg aaggaccgcg gcggacgcgg gcgctccggc
     1081 cgcaaattcc aaatcaaaca atagggtccc ggcggccttt tggccggggg ccgacgaaac
     1141 gacaaaaacg atttctttca aagccccgat gcgaccccaa tcaatcgccg attcggtccg
     1201 attccacccg gcgcgcagga cgagggggag gcgttgaact tcccggacgc cctttatctc
     1261 gagggccacg gaagcgggcg gcggaaccgt cggccctttg accgaaacga ccacgcgctg
     1321 gaccgccccg gcgttcaccg tcgagggata atccttggtc cacacgccca ccaacgcgcc
     1381 ggccggcgcg gtgaaatcaa attttaaaac cggtttctgg aggtccggat cctggagcgt
     1441 gttgacggtg ccctgggaag ggccgatgtt gaacacc
//
LOCUS       DAOQLS010000589         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_70290_length_1469_cov_3.398868, whole genome shotgun sequence.
ACCESSION   DAOQLS010000589 DAOQLS010000000
VERSION     DAOQLS010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70290_length_1469_cov_3.398868"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PK362_09740"
     CDS             <1..486
                     /locus_tag="PK362_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC75148.1"
                     /translation="VLTVGTIHGGERFNILPSSVVLTGTVRTLTPEWHRRMPRLIRQI
                     AQGVARAHGGRARVDYEVLSRPVVNDRAMTALARRAAEETVGARRALVLERPSMGGED
                     FSEYLSKAPGCFVYVGTGADARTRRPWHHASFVLHEPSMLTGVNFLTALARRALRELK
                     G"
     gene            483..>1469
                     /gene="thiL"
                     /locus_tag="PK362_09745"
     CDS             483..>1469
                     /gene="thiL"
                     /locus_tag="PK362_09745"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNC75149.1"
                     /translation="MSPAAKGSGPNTLGRWGEWKWLSALLPGLSRGLSRRVVLGPGDD
                     AALLNLGGETWAITTDMLVEGVHFRTSWTSGEELGHKALAVNLSDLAAMGDVTPGFGV
                     VSAGFPPQTPVSFLKGFYRGFAALARSTGFDLVGGDTVRADRITVSVTALGKVRPGNR
                     VFRRDAFHPGDVLMVTGTLGDADAGLSIAEARRPPRTADERFLLKRFRRPAPRLDAAR
                     RLARAGVRAGLDSSDGLWRSVKILCERSGVGAAVDVERLPLSPALRRWAGPGAFRRAL
                     TGGEDYELVVGASPSTAGRLERLGWATAVGTILPRASGVGLFKGKDKREAPAG"
BASE COUNT          182 a          450 c          562 g          275 t
ORIGIN      
        1 gttctgaccg tcggcacgat ccacggcggg gagcgcttca atattttgcc gtcgtcggtc
       61 gtcttgacgg ggacggttcg cactttgacc cccgaatggc accgccggat gccccgcctg
      121 attcgtcaaa tcgcgcaggg ggtggcccgg gcccacggag ggcgggcccg cgtggattac
      181 gaggtgttga gccggccggt ggtcaacgac cgggccatga cggcgctggc ccgtcgcgcg
      241 gcggaggaaa ccgtgggggc ccgtcgcgcc ctggttttgg aacggccgtc catgggcggc
      301 gaggattttt cggaatattt gtcgaaggcc ccgggttgtt tcgtctacgt cggaacgggc
      361 gcggacgccc gcacccgccg gccctggcac cacgcttcct tcgtgctgca cgagccgtcg
      421 atgctgaccg gcgtgaattt tttgacggcc ctcgcgcgcc gggcgctccg ggaactgaag
      481 ggttgagccc cgcggcgaaa gggtccgggc cgaacacgct cgggcgctgg ggggaatgga
      541 agtggctctc ggcgcttttg ccgggtttgt cccggggtct gtcccgccgg gtggtcctgg
      601 ggcccggcga cgacgccgct ctgttgaatt tgggcggtga aacctgggcc atcaccaccg
      661 acatgctggt ggaaggcgtt cactttcgaa cgtcctggac ctcgggcgag gagttgggcc
      721 acaaggccct cgccgtgaat ctatcggatt tggccgccat gggcgacgtg acccccgggt
      781 tcggcgtggt gtcggcgggt tttccgcccc aaacgcccgt gtcctttttg aaggggtttt
      841 accggggatt tgcggccctc gctcggtcga cgggtttcga tttggtcggc ggcgacacgg
      901 tgcgcgccga ccggatcacg gtgtcggtga cggcgttggg aaaggttcgg cccgggaacc
      961 gggtcttccg ccgggacgct tttcacccgg gggacgtgtt gatggtcacg ggcacgctgg
     1021 gcgacgccga cgcgggcctc tcgatcgcgg aagcccggcg gccgccccgg accgcggacg
     1081 agcggttcct gttgaaacgc tttcgacgtc cggcccctcg gttggacgcc gcgcgccggt
     1141 tggcccgggc gggggttcgg gcgggactcg acagttcgga cgggttgtgg cggtcggtaa
     1201 aaatactttg cgagcgttcc ggggtgggcg ccgcggtgga cgtcgaacgt ttgccgctct
     1261 cgcccgcgct ccggcgatgg gccgggcccg gggcgttccg ccgggccttg acgggcggcg
     1321 aggattacga gttggtcgtc ggcgcttcgc cgtcgacggc cgggcgtctc gagcgattgg
     1381 gttgggcgac cgcggtggga acgattcttc cccgcgcgtc gggcgtcggg ttgttcaaag
     1441 gcaaagacaa acgggaggct cccgctggc
//
LOCUS       DAOQLS010000590         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_70375_length_1468_cov_4.364473, whole genome shotgun sequence.
ACCESSION   DAOQLS010000590 DAOQLS010000000
VERSION     DAOQLS010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70375_length_1468_cov_4.364473"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1468
                     /locus_tag="PK362_09750"
     CDS             <1..>1468
                     /locus_tag="PK362_09750"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNC75150.1"
                     /translation="TRGHESARNPKPLQIIELRLATAARAAQNARNQPPSTPGRGTPR
                     ALLTTLLVGPLAFLPASGAWAADGALRASTDWLSFIGVTVSVAALALGGLLVLAYLSV
                     SPKMPAPLQKFGSAVLNLLNLRRRTPPTPAATPAAPAVTLPELIQRIDGLSNPVLGVL
                     KANLEAGVVATPEALAAVSVLLDAGQASLFAKWDAPGTHDDSKRGFLAQVAKMNAEYP
                     GGLTTYAANARVLLEKSARGDNPFKGKTPNAPPMTNLTGRMDEVERYQTAALPSANNL
                     AFVLMAGGMGERLGYNGIKISIPLDLVTETTYMGWYIRHILALQEASNRKTGESKKIP
                     LFIMTSEENHDVTMAFLAEHDYFGMDKEQIIVRQQPLVPAVQNNNADFALDADDPYRL
                     QGKPHGHGDVHMLLHHNGVVKQWAREGRSHVVFIQDTNGQVFNGLLAAVGVSMEKDLD
                     VNFLTVDREAGEKTGSITTLVGADQSAVTGNVEYNEIDP"
BASE COUNT          287 a          492 c          440 g          249 t
ORIGIN      
        1 acccgggggc atgaatcggc gcggaacccc aagcccctcc agatcatcga gttgcgcctg
       61 gcgacggcgg cccgcgccgc ccagaacgcc cgaaatcaac ccccttccac ccccggccgc
      121 gggacccccc gggcccttct gaccacgttg ttggtcgggc ccctggcgtt tcttccggcc
      181 tccggagcct gggcggccga cggcgccctg cgtgcgtcga cggattggtt gagcttcatc
      241 ggcgtcaccg tgtccgtggc ggccctcgcc ctgggcggcc tgcttgtgct ggcctacctc
      301 tccgtttcgc ccaaaatgcc cgcccctttg cagaaattcg ggagcgccgt tttgaatctg
      361 ttgaaccttc ggcgccgaac acccccaaca ccggccgcga cgcccgccgc ccccgcggtg
      421 acactcccgg agttgatcca acgcattgac ggcctgtcca atccggtctt gggcgtcctt
      481 aaagccaacc tggaagccgg ggtggtggcc acgccggaag cgctggccgc ggtttccgtt
      541 ttgctcgacg cggggcaagc gtcgttgttc gccaagtggg acgccccggg cacccacgac
      601 gattccaaac gggggttctt ggcccaagtg gccaaaatga acgccgaata tccgggggga
      661 ctcacgactt acgcggccaa cgcccgcgtg ctgttggaga agtccgcccg gggcgacaac
      721 ccgtttaaag gaaaaacgcc caacgcgccg cccatgacga atctcaccgg gcggatggac
      781 gaggtggaac ggtatcaaac ggccgcgttg ccttcggcca acaatttggc ctttgtgttg
      841 atggccggcg gcatgggcga acggctgggt tacaacggaa tcaagatttc cattcccctc
      901 gacttggtga ccgagaccac gtacatggga tggtacatcc ggcacatcct cgcccttcag
      961 gaagcgtcga accgaaaaac cggcgaatcg aaaaaaatcc ccctgtttat catgacgtcc
     1021 gaggaaaacc acgacgtcac catggccttc ctggccgaac acgactactt cggcatggac
     1081 aaggaacaga tcatcgttcg ccaacagccg ctggtgcccg cggtccaaaa caacaacgcc
     1141 gatttcgctt tggacgccga cgacccctac cggctccagg gcaagcccca cggccacggc
     1201 gacgtccaca tgttgctcca ccacaacggg gtggtgaaac aatgggcgcg ggagggtcgg
     1261 tcgcacgtgg tgttcattca ggacacgaac ggccaggtgt tcaacggtct tctggcggcg
     1321 gtgggcgttt ccatggaaaa ggatttggac gtcaacttcc tcaccgtgga ccgggaagcg
     1381 ggcgaaaaaa cggggtccat caccacgctg gtcggcgcgg accaatccgc ggtcacgggc
     1441 aacgtcgaat acaacgagat cgacccct
//
LOCUS       DAOQLS010000591         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_70392_length_1468_cov_3.344657, whole genome shotgun sequence.
ACCESSION   DAOQLS010000591 DAOQLS010000000
VERSION     DAOQLS010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70392_length_1468_cov_3.344657"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1468
                     /locus_tag="PK362_09755"
     CDS             21..>1468
                     /locus_tag="PK362_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC75151.1"
                     /translation="MALQVGSVTGDARVTPVRLVGTLKNLEEFRKVVVARDPSGGTVL
                     LENVATVTDSYLEPESLSRLNGQAAVSLYVQKESSANTLRVVRDLEKAWDKAWRNLPA
                     GTRKDLRQVVVSNQAVGILAAVVSVRVSLACGILLILLSLCLVESERLSTKRMAGVVL
                     GGLLALMCVTALLKISDARMEPLYWILLGLFLLLAFFDPDIRPGLIVAGSMPLSALFC
                     FLLFQACGLTLNVMSLFCLALGLGMLVDNATVVYEHINELGLPPPGPAAREAALKATE
                     EMVIPLIGGTVTNAIVFVPFLFLSREIQLMYTDVAAAVGASLFASLGISLTAVPLLAS
                     GLVLKGPPKWPTFRGALESRAKKWGEDLNRLGTLMWERGAAFSKRALERARAFYAARL
                     EPRLNSLAGHHSLAGLPPWAGLVLFVVLFAGLWALGGKTPAKIFFLLTALGLTAAGFA
                     LFRGFEGHWRRLFHRRVEILGAALLLAAAATAV"
BASE COUNT          212 a          468 c          465 g          323 t
ORIGIN      
        1 gccttctggg ccgacgaaac gtggccctgc aagtcggaag cgtcaccggc gacgcccggg
       61 tgaccccggt gcggctcgtg ggcaccctta aaaaccttga ggaattccga aaggtcgtgg
      121 tggcccggga cccctcgggc ggcaccgtcc tgctggaaaa cgtcgccacc gtcaccgaca
      181 gttacctgga accagagagc ctctcccgtt tgaacggcca agccgccgtg tccctttacg
      241 ttcaaaagga atcctccgcc aacaccttga gggtggtgcg cgacctggaa aaagcctggg
      301 acaaggcctg gcgcaacctt cccgcgggaa cgcggaaaga ccttcgccaa gtggtcgtct
      361 ccaaccaggc cgtggggata ttggcggcgg tggtatcggt tcgcgtgtcc ctggcctgtg
      421 ggattctctt gattctcctt tccctctgcc tggtggaaag cgaacgcctt tccaccaagc
      481 gcatggcggg cgtcgtcctg ggcggcctgt tggcgctcat gtgcgtcacc gccctcctga
      541 aaattagcga cgcccgcatg gagcccctct actggatcct cctggggcta tttctcctat
      601 tggctttttt cgacccggac atccggcctg ggctcatcgt ggcggggagc atgccccttt
      661 cagcgctttt ttgctttctg ctcttccagg cctgcgggtt gaccttgaac gtcatgagcc
      721 tcttctgcct ggccctgggg ctcggcatgt tggtggataa cgccacggtg gtctatgaac
      781 acatcaacga actgggcctc cccccgcccg gccccgccgc ccgggaggcg gcgctcaagg
      841 cgacggagga aatggtcatc cccttgatcg gcggcaccgt caccaacgcc atcgtcttcg
      901 ttccctttct tttcctctcc cgggaaatcc agttgatgta caccgacgtg gcggcggccg
      961 tgggcgcctc cctcttcgcc tccctgggca tttcattgac ggcggttccc ctcctggctt
     1021 ccggcctggt tcttaaaggc cctccgaaat ggccgacgtt tcggggggcg ctggaatccc
     1081 gggcgaagaa atggggcgag gacttaaaca ggttggggac gcttatgtgg gagcgcggag
     1141 cagccttctc aaagcgggct ctggagagag cccgggcctt ttacgccgcc cgccttgagc
     1201 cccgtctcaa ctcactggcc ggccatcatt ccctggcggg tctgccgccc tgggcggggt
     1261 tggttttgtt tgtggtgttg tttgcggggt tgtgggcgct tgggggcaag acgccggcca
     1321 agattttctt tctgctcacg gccctggggc tcacggcggc gggcttcgcc cttttccggg
     1381 gttttgaggg ccattggcgg cggctctttc accgtcgggt tgaaattctg ggagcggcgc
     1441 tgttactcgc ggcggccgcc acggcggt
//
LOCUS       DAOQLS010000592         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_70682_length_1465_cov_3.065248, whole genome shotgun sequence.
ACCESSION   DAOQLS010000592 DAOQLS010000000
VERSION     DAOQLS010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70682_length_1465_cov_3.065248"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PK362_09760"
     CDS             complement(<1..261)
                     /locus_tag="PK362_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75152.1"
                     /translation="MTVPDRPLWQRPLLWAWAFYDFANSAFATTTLAVVFNVYFAKSV
                     VPPEGLRILGRVLSGPALWSFTVSASMFVMVLLAPLLGAVADL"
     gene            complement(258..1157)
                     /locus_tag="PK362_09765"
     CDS             complement(258..1157)
                     /locus_tag="PK362_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC75153.1"
                     /translation="MTPEAHHRHALSTARPRRVLVFAATLTGATFLLELWGAHRSGSV
                     ALLSDAAHVFMDFGGLLFSYFAVTLAERPASDRRTFGLHRLEVLAAVANGLLVSGVAL
                     GIAWKSLHRFHGPAALPGTGWMMGLGLAGLAANIAVAAALHGPSRTDVNLRGSFLHVA
                     SDALASVGVVAAGALIAATGWRAVDPLVGLMIAALILGSALPLLRESLNLLLEGVPAG
                     LHLSEVTEALQSLPGVRRVEDVHVWGLCSHLVSLSARVILDPARMAEQPRTLSALQTL
                     LRDRFSIGHSTLQLEPAEPDARP"
     gene            complement(1154..>1465)
                     /locus_tag="PK362_09770"
     CDS             complement(1154..>1465)
                     /locus_tag="PK362_09770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC75154.1"
                     /translation="HTGLDLAAPSGTPVTAPQRGVVVFAGRIESFGNTVLLDHGQGVY
                     TYYLHMRALAVRAGDRVKTGRLLGETGAEGIATGPHVHWSLTVQGERVDPQDWVDRFI
                     P"
BASE COUNT          279 a          459 c          554 g          173 t
ORIGIN      
        1 caaatccgcc accgcgccca gcaagggggc caacaacacc atcacgaaca tggaggccga
       61 caccgtgaag ctccaaaggg ccgggcccga aaggacgcgc cccaggatcc ggaggccttc
      121 gggcggaacg acgcttttgg cgaagtagac gttgaagacg accgccaggg tcgtggtcgc
      181 gaaggcggag ttggcgaaat cgtaaaaagc ccaggcccag aggagcgggc gttgccacag
      241 cgggcggtcc gggaccgtca cgggcgggcg tcgggctcgg cgggttccaa ctgcagggtg
      301 gaatggccga tggaaaatcg gtcccgcagc agggtctgga gggcggacaa cgttcggggt
      361 tgttcggcca tgcgcgccgg gtccaggatc acccgggcgg agagggacac gagatgggag
      421 cagaggcccc acacgtgaac gtcctccacg cggcggacgc ccggaaggct ttgaagggcc
      481 tccgtcactt ccgacaaatg gagccccgcg gggacgccct ccaggaggag gttcaaggat
      541 tcccgaagaa gggggagggc gctgccgagg atcaacgcgg cgatcatcag accgacgagc
      601 gggtccaccg cccgccaccc cgtggcggcg atgagggcgc ccgcggcgac cacgccgacg
      661 ctggccaggg cgtccgaggc cacgtgcaga aaggagcccc gcaaattcac gtcggtgcgg
      721 ctgggtccgt gaagggccgc ggccacggcg atgttggccg ccagtccggc caagcccagc
      781 cccatcatcc aaccggtgcc cgggagcgcc gcgggtccgt ggaatcggtg gagggatttc
      841 caggcgatgc ccagggccac gcccgacacc aaaaggccgt tggccacggc ggccaggact
      901 tccagccgat gcaggccgaa ggttcgccgg tccgaggcgg ggcgttcggc cagggtcacg
      961 gcgaaatagg aaaagagaag gccgccgaag tccatgaaga cgtgggcggc gtccgagagg
     1021 agggcgacgg agccgcttcg gtgggcgccc cagagttcca gaaggaaggt cgcgccggtg
     1081 agggtggccg cgaacacgag aacccgccgg gggcgggccg tggaaagggc gtggcggtga
     1141 tgggcctcgg gggtcacgga atgaaccgat ccacccaatc ctggggatcc acgcgctccc
     1201 cctggacggt gagggaccaa tggacgtggg ggcccgtggc gatgccttcg gcgccggttt
     1261 cacccaacaa gcggccggtc ttgacccggt ccccggcccg gaccgcgagg gcgcgcatgt
     1321 gcaggtaata cgtgtagacg ccctggccgt gatccagcag caccgtgttt ccgaaacttt
     1381 cgattcgtcc cgcgaacacg acgacgcccc gctggggggc cgtgacgggg gttcccgagg
     1441 gggcggcgag gtccaaaccc gtgtg
//
LOCUS       DAOQLS010000593         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_71317_length_1458_cov_3.742694, whole genome shotgun sequence.
ACCESSION   DAOQLS010000593 DAOQLS010000000
VERSION     DAOQLS010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71317_length_1458_cov_3.742694"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PK362_09775"
     CDS             <1..432
                     /locus_tag="PK362_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75155.1"
                     /translation="AQWFRRGETTRLLVPLTIAPSGDRLRLTVDNGDNAPLTLANFRV
                     HYRTYALVFKARAGDPMTLHYGNPGVNAPAYDAAAVESELLAADKVDAVLELPEKGSG
                     VRWIPSGAGGGPVLWAALILATGVLLWVIAKLLPPPGNGKP"
     gene            452..1255
                     /locus_tag="PK362_09780"
     CDS             452..1255
                     /locus_tag="PK362_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNC75156.1"
                     /translation="MKIPRFSRGVGGAATAAVLLAAVGFFQRPSGSRSWAPEFAVPTR
                     FEFSPAGVKITALRDYAWKQEGGTPRERTVYVAWDDVVESRLLVSPLSPGRRGPAHVM
                     MGFGLRDGTDLVVSAEARREGGEAYSAWRGALRHYELTYVVSTEADAVSLRTVHRGAP
                     VYQYRLRADPPALRALFRDMLERADALSRRPEFYNTLFNNCAQNIRRHANRLVDRPFG
                     RSWRFLLPGYLDAEAASRGLLELEGPLDQVREAHRMDLSVESSATRGPA"
     gene            1252..>1458
                     /locus_tag="PK362_09785"
     CDS             1252..>1458
                     /locus_tag="PK362_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75157.1"
                     /translation="MSRPAPLLLRHRGFLALTLAQFFETLNDSAFKTLVTFWALAKFG
                     AEQGGRGAALVSGLFILPFLLFSTY"
BASE COUNT          212 a          506 c          463 g          277 t
ORIGIN      
        1 gcccagtggt tccggcgggg ggaaaccacc cgcctgttgg ttcccctgac catcgcgccc
       61 agcggcgatc gacttcgcct gacggtggac aacggcgaca acgcgcccct gaccctggcc
      121 aattttcgcg tccattaccg gacctacgcc ctggttttca aagccagggc gggggaccca
      181 atgaccctcc actacggaaa tcccggggtg aacgcccccg cctacgacgc cgccgccgtg
      241 gagtcggagt tgctggccgc cgacaaagtg gacgccgtct tggaattgcc ggaaaagggg
      301 tcgggcgttc gctggatccc gagcggcgcg ggcggcggcc ccgtcctctg ggcggcgctg
      361 atcctggcca cgggcgttct cctgtgggtc atcgccaaac tgttgccgcc ccccgggaac
      421 gggaagccct aaatcctccg gcgccgattc cttgaaaatc cctcgattct cccgcggggt
      481 cggcggggcc gcgacggcgg cggttctttt ggccgccgtc ggctttttcc aacgtccctc
      541 ggggtcccga tcctgggccc cggaattcgc cgtcccgacg cggttcgagt tttccccggc
      601 gggggtgaaa ataaccgccc ttcgggacta tgcttggaag caagagggag gaaccccccg
      661 tgagcgcacc gtttatgtcg cctgggacga cgtcgtggag tcccggttgt tggtgtcccc
      721 gctgtcgccc ggacgccggg ggccggccca cgtgatgatg ggattcggcc ttcgcgacgg
      781 aacggacctc gtcgtttccg ccgaagcccg gcgggagggc ggggaagcct attccgcctg
      841 gcggggcgcc cttcggcact acgaattgac ctacgtggtt tcgaccgagg cggacgcggt
      901 gtcccttcga acggtccacc ggggcgcgcc cgtgtatcaa taccgtctgc gggcggatcc
      961 ccccgccctc cgcgccctct ttcgggacat gctggaacgg gccgacgcgc tttcacggcg
     1021 ccccgagttt tacaacaccc tcttcaacaa ctgcgcccaa aacattcgcc gccacgccaa
     1081 ccgtttggtg gaccgcccct tcggccggag ctggcggttt cttctccccg gctatctgga
     1141 cgcggaagcc gcgtcccggg ggttgctgga actggaaggc ccgttggatc aagtccgcga
     1201 ggcccatcgg atggatcttt ccgtcgaatc gagcgcgacg cgaggccccg cttgagccgt
     1261 ccggcgcccc tgctgctccg tcaccggggt ttcctggccc tgaccctggc ccaatttttt
     1321 gaaacgctga acgacagcgc tttcaaaacc ctggtcactt tctgggctct ggcaaaattc
     1381 ggcgccgaac aaggcgggcg gggagcggcg ctcgtgagcg gtctgttcat ccttcccttt
     1441 cttctctttt ccacctac
//
LOCUS       DAOQLS010000594         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_71683_length_1454_cov_3.749821, whole genome shotgun sequence.
ACCESSION   DAOQLS010000594 DAOQLS010000000
VERSION     DAOQLS010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71683_length_1454_cov_3.749821"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PK362_09790"
     CDS             complement(<1..209)
                     /locus_tag="PK362_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC75158.1"
                     /translation="MAKSIVIIGGGPGGEAAAKRAAARGARVTLVEKNRLGGVCLNWG
                     CIPSKALLEAGRLSHAVSAAGATTSG"
     gene            complement(213..1046)
                     /locus_tag="PK362_09795"
     CDS             complement(213..1046)
                     /locus_tag="PK362_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75159.1"
                     /translation="MRLFRLGGTFFLIGIGAAPVAAADPPKALGATLNAEAVLRLLGG
                     RYDAEEGAARLRIPMDGGEEAECRAYLLDVAAWSAEGKVRALFLTKTLCAGHDAHVDG
                     ALLGGGVLEKREGRWAPVNVSPTIAEFGAFGDFRGEVSTVPLGERRIGFLLRMERMHQ
                     GVAAGVLSLWGTRDDRLAEILPPVVVSEDGCAGQECVDTVKEQDRFEKQVGYRPPLRW
                     AFGSELRWSAAAGAGWNELVVITTGTESDWTEDRQTVRPVRRTRRFAFSEADGVYVEQ
                     P"
     gene            complement(1064..>1454)
                     /locus_tag="PK362_09800"
     CDS             complement(1064..>1454)
                     /locus_tag="PK362_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNC75160.1"
                     /translation="LEGALKLKEISYIHAEGYPSGELKHGPIALIDEDMPVVAIATPS
                     PVYDKMMSNLEEVAARGGKVIALAARGDARVAALTPDVISMPDVPDYLSPMVNVVPLQ
                     LLAYHIAVLRGCDVDQPRNLAKSVTVE"
BASE COUNT          240 a          518 c          468 g          228 t
ORIGIN      
        1 cccgacgtgg tggcccccgc ggcggacacg gcgtgggaca aacgcccggc ttccagcagg
       61 gctttggacg ggatgcatcc ccaattgagg caaacgccgc ccaagcggtt tttctccacc
      121 agggtgaccc gggcgccccg ggcggcggcg cgtttggcgg cggcctcgcc gccgggtccc
      181 ccgccgatga tcacgattga tttcgccata aatcagggtt gctccacgta gacgccgtcc
      241 gcctccgaaa aagcgaaccg ccgcgttcgt cgaacgggcc ttacggtttg ccggtcctcc
      301 gtccaatcgg attcggtgcc cgtggtgatc accaccagct cgttccaccc cgcccccgcc
      361 gccgccgacc aacggagctc ggaaccaaag gcccaccgaa ggggcgggcg gtagcccacc
      421 tgcttttcaa agcggtcctg ctctttgacg gtgtccacgc actcctggcc cgcgcacccg
      481 tcttcggaaa cgacgacggg gggaaggatt tccgccaacc gatcgtcgcg cgttccccac
      541 agggacagga caccggcggc gacgccttga tgcattcttt ccatccggag cagaaatccg
      601 atccggcgct ccccgagggg gaccgtcgac acctcgcccc gaaaatcgcc gaaggccccg
      661 aactcggcga tggtggggct gacgttcacg ggcgcccaac gcccctcccg tttttccagg
      721 acgccgccgc cgagcaaggc gccgtccacg tgggcgtcgt ggcccgcgca aagggttttg
      781 gtgagaaaga gggcgcgtac ttttccctcg gcggaccagg cggcgacgtc cagcaaatag
      841 gcccggcatt cggcctcttc gccgccgtcc atgggaattc ggagccgcgc ggcgccttcc
      901 tccgcgtcat agcggccccc cagaagcctc aaaacggctt cggcgttgag cgtcgccccc
      961 aaggccttgg gcggatcggc cgccgcgacc ggggccgcgc cgatcccgat caaaaagaac
     1021 gtcccgccca accgaaagag gcgcacgcgc ggaacccggc gcgttattcg accgtgacgg
     1081 atttggccag gttgcggggc tggtccacgt cgcacccgcg cagaacggcg atgtggtagg
     1141 cgaggagttg aaggggcacg acgttcacca tggggctcaa gtaatccggc acgtccggca
     1201 tcgagatcac gtcgggggtc agcgccgcca cccgggcgtc gccccgggcc gcgagggcga
     1261 tcacttttcc gccccgggcg gccacctcct ccaggttgga catcatcttg tcgtacaccg
     1321 gcgacggcgt cgcgatggcc accaccggca tgtcttcgtc gatcaaggcg atgggcccgt
     1381 gcttgagctc gcccgacggg tacccctcgg cgtggatgta ggaaatctcc ttcagcttga
     1441 gggcgccttc gagc
//
LOCUS       DAOQLS010000595         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_71920_length_1452_cov_3.005011, whole genome shotgun sequence.
ACCESSION   DAOQLS010000595 DAOQLS010000000
VERSION     DAOQLS010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71920_length_1452_cov_3.005011"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..538
                     /locus_tag="PK362_09805"
     CDS             140..538
                     /locus_tag="PK362_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNC75161.1"
                     /translation="MSPSYDWYSQLKKPAWSPPAGIFGPVWTVLYIGIAVSFGHVFYK
                     TATRQWPAVVALPFALNLIFNLAFTPLQFGLKSNPLAAVDILLVLGTLLWSMIKVQPH
                     SSWVFYAQIPYLLWVSFATVLQLTITFLNR"
     gene            566..>1452
                     /locus_tag="PK362_09810"
     CDS             566..>1452
                     /locus_tag="PK362_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNC75162.1"
                     /translation="MNFDAAPTPAHWKNRLLAYYRRHRERLNVAFFLGGFLFDVVTLS
                     TVDNPWGIAQQLAYLGAIGWLLSKELLFEAKLWAPSKRLEKVWAYRVLLVHFMLGSLI
                     SLYSLFFLKSASLASSLLFIALLLGVLVANELPRVQSAGLGLKVALYALCLFSFFSML
                     WPTFLGFVGRVPFALSLASAGAAVASMALWVYRRFPNPRQVSRRILVPGAVLGALTVA
                     FYFLGWIPPVPLAVVDMGIYHNVEKRGDRYWLFHERPTWALWRKGDQDFVARPGEAVY
                     FFARIYSPARFSDELTLHW"
BASE COUNT          212 a          447 c          411 g          382 t
ORIGIN      
        1 atgcactctc ctgggtcggg tttatgcgaa tgagcgggag atgagggccc cggtgccgaa
       61 ggttgattct accaattggg tccggaaaag gcccggtgtt cccgggcccc ttctttaaaa
      121 tattggtaag ctcatggccg tgagcccttc ctacgactgg tattcgcagt tgaagaaacc
      181 cgcgtggtcc ccgccggccg gaatattcgg tccggtgtgg acggtgttgt atatcggaat
      241 cgccgtttcc tttggccatg ttttctataa aaccgccacg cgccaatggc cggccgtcgt
      301 ggccctcccc ttcgccctga acctcatttt taatttggcc ttcacgcccc tccaattcgg
      361 tttgaaatcc aaccccctgg cggccgtgga tatccttttg gttttgggga cgcttctgtg
      421 gtccatgatc aaagtgcaac cccacagctc ctgggtgttt tacgcccaga tcccctatct
      481 gctctgggtg tctttcgcca cggtactgca gctcacgatc actttcctga accgttagtt
      541 cctttcggtc catggaaccc ctcccatgaa tttcgacgcg gctcccacgc ccgcccattg
      601 gaaaaaccga ctgttggcct attaccgacg gcacagggag cggctcaatg tcgctttttt
      661 tctcggtggt ttcctcttcg acgtcgtcac cctgtccacc gtggacaacc cctggggcat
      721 cgcccaacag ttggcttatc tgggcgcgat cggctggttg ctctccaaag aactcctctt
      781 tgaagcgaag ctttgggcgc cgtccaaacg gttggaaaaa gtttgggctt accgggtgct
      841 cctggtgcat ttcatgctgg gaagcctcat cagcctatac tccctcttct tcctcaaaag
      901 cgcgtcgctg gccagttcgc ttcttttcat cgcccttctt ctgggcgttt tggtggccaa
      961 cgagctcccc cgggtgcagt cggccgggtt gggactcaag gtcgcgcttt acgccctttg
     1021 cctgttctcc tttttttcca tgttgtggcc caccttcctg gggttcgtgg ggcgggtgcc
     1081 cttcgccctc tccctggcga gcgcgggggc ggcggtggcc tccatggctc tttgggtgta
     1141 ccgtcgattt ccgaatccgc gccaggtgtc gcggcggatc ctggtgcccg gcgccgtcct
     1201 gggcgctttg acggtggcgt tttatttcct gggatggatt ccgccggtgc ccctggcggt
     1261 ggtggacatg ggcatttacc acaacgtcga aaagcggggc gaccggtatt ggctttttca
     1321 cgagcggccg acctgggccc tttggcggaa gggcgaccag gatttcgtcg cccggcccgg
     1381 cgaagccgtt tattttttcg cccggatcta ttcgcccgcc cgcttctcgg acgagttgac
     1441 gctccactgg ta
//
LOCUS       DAOQLS010000596         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_71978_length_1451_cov_6.118195, whole genome shotgun sequence.
ACCESSION   DAOQLS010000596 DAOQLS010000000
VERSION     DAOQLS010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71978_length_1451_cov_6.118195"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PK362_09815"
     CDS             complement(<1..623)
                     /locus_tag="PK362_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75163.1"
                     /translation="MSFIYPLLLSLCLALAAGSAAAEPGASWLRAANPGALNPKISVI
                     ADFVGQTGTAGDPSQTEDGFHLREVEIGVQADIDPYARADFFIGGMNDAGSPPELEEG
                     YFTLLSLPGGFQARGGKFRANFGRLNMVHPHELPQVDSPLVLDAFLGEERLASTGFEL
                     SRIFAPFDLYSEASYALLQDLGATPALPGHTITVTDQKTGLPVDLALP"
     gene            complement(686..973)
                     /locus_tag="PK362_09820"
     CDS             complement(686..973)
                     /locus_tag="PK362_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75164.1"
                     /translation="MRHRPFLLALGLLALLGFQAVHRHTGHESGFYKPHVDCSLCAWT
                     GGAGARTGAAAPNAVRVSWTALPAAPDRVGSDFFLPYTLPFGRGPPALPSI"
     gene            complement(1005..>1451)
                     /locus_tag="PK362_09825"
     CDS             complement(1005..>1451)
                     /locus_tag="PK362_09825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC75165.1"
                     /translation="TANFIGLAEGTKEFTDPLTNKKAKRPYYEGVIFHRVIDGFMIQG
                     GDPTGTGRGGPGYEFPDEIDPKQEFDKKGQVAMANRGPDTNGSQFFITLGAVGLPKNY
                     TIFGQVVSGQSVVDAIGKMPTSGPPNDRPLQPPVIRHAKIVRVGKS"
BASE COUNT          274 a          444 c          496 g          237 t
ORIGIN      
        1 ggcagggcca ggtccacggg caatccggtt ttctggtccg tcaccgtgat cgtgtggccg
       61 gggagcgcgg gcgtcgcgcc caaatcctgg agaagggcgt aggacgcttc cgagtagagg
      121 tcgaagggcg cgaagatccg gctcagctcg aacccggtgc tggccaagcg ctcctcgccc
      181 aaaaaagcgt ccagcaccag gggcgaatcg acctggggaa gctcgtgggg atgcaccatg
      241 ttgagccgac cgaaattcgc gcggaacttc ccgccccggg cctgaaaacc gccggggagc
      301 gaaagcaggg tgaagtaccc ctcctccagt tccggcggcg acccggcgtc gttcatcccg
      361 ccgatgaaaa aatcggcgcg ggcgtaggga tcgatatcgg cctgaacgcc gatttcaacc
      421 tcccgaagat gaaagccgtc ctcggtttgg ctgggatccc cggcggtgcc ggtttgtccg
      481 acgaagtcgg caatgacgct gatcttgggg tttaaagccc cggggttcgc ggcgcgaagc
      541 cacgacgccc ccggttcggc ggccgccgac ccggccgcca gcgccaggca aagcgataaa
      601 agcaaaggat agataaagga catgggtcct ccctatgagg ggttcaaggc gcgcaaaatg
      661 cgcggtccct aggaacgaac ttgaatcaga tggaaggaag ggcggggggc cctcgcccga
      721 aggggagggt gtagggaagg aaaaaatccg aaccaacgcg gtccggggcg gcgggcaggg
      781 cggtccaaga aacccggacc gcgttgggcg ccgccgcgcc cgtgcgtgcg ccggcgcccc
      841 cggtccaggc gcacagggag caatccacgt gcggtttgta aaagccgctt tcgtggcccg
      901 tgtggcggtg gaccgcctga aagccaagca gggccagcag acccagggcc agcaggaagg
      961 ggcgatgtcg catggttata gattaccccc gacgggggcg tccgtcaaga ttttccgacg
     1021 cgcacgattt tggcgtgccg aatcaccggc ggctgcaggg gccggtcgtt cggaggcccc
     1081 gaggtcggca ttttgccgat ggcgtccacc accgactgcc ccgacaccac ctgtccgaag
     1141 atggtgtagt tcttgggaag acccacggcg cccagggtga tgaagaactg ggacccgttg
     1201 gtgtccgggc cgcggttggc catggccacc tggccttttt tatcgaactc ctgcttcggg
     1261 tcgatctcgt ccgggaattc gtagcccggg ccgccccggc cggtgcccgt ggggtcgccg
     1321 ccctggatca tgaacccgtc gatcacccgg tggaaaatca cgccctcgta ataggggcgc
     1381 ttggctttct tgttcgtcaa cggatccgtg aattccttgg tcccttcggc caaaccgatg
     1441 aaattcgccg t
//
LOCUS       DAOQLS010000597         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_72156_length_1450_cov_1.945520, whole genome shotgun sequence.
ACCESSION   DAOQLS010000597 DAOQLS010000000
VERSION     DAOQLS010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72156_length_1450_cov_1.945520"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PK362_09830"
     CDS             <1..413
                     /locus_tag="PK362_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75166.1"
                     /translation="LCGLAGSYFFLAPPSRGGLGGGALGLSVKLLVFNVATTNVLLWV
                     IARSLNMRFGYYVRHQVVTVLFWTALAAASGAAAARGIRSPVAAFAAAAVLYGALAAA
                     VLSLRPQWVGVAPTDARRLFSWVGAALRGRPRKI"
     gene            432..1277
                     /locus_tag="PK362_09835"
     CDS             432..1277
                     /locus_tag="PK362_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC75167.1"
                     /translation="MESKDLTWKERIRRRSLEAAQREQGLTDLVGRLREIVPDIAGQE
                     SSEEELFNPYWEVKRRGLQAFQCREMLAAVDALGRAPGAGLTVVDIGDSAGTHLLYLK
                     ALLGPARPVETVSVNLDPRAIAKIQARGMKAVLCRAEDLRLDRPVDLFVSFEMLEHLH
                     NPALFLRRLACRDDQSRLLLTVPYRRRSQVGLHHVRRNDLKTVHAEEEHVFELSPGDW
                     TLLFRHAGWRVARSATYRQYPRRWNPAAPLWAWWWNRSDFEGFWAVLLERDRSLSDRY
                     VDWEK"
BASE COUNT          195 a          480 c          504 g          271 t
ORIGIN      
        1 ccctgtgcgg cttggcgggg tcgtattttt ttctggcgcc gccctcccgg ggcggattgg
       61 gcgggggcgc cctgggtttg tccgtcaaac tccttgtctt taacgtggcc accaccaacg
      121 tccttttgtg ggtcatcgcc cggtccctga atatgcggtt cgggtactac gttcgccatc
      181 aggtggtgac ggttcttttc tggaccgccc tggcggccgc gagcggcgcg gcggcggccc
      241 ggggaatccg ctctcccgtg gcggcgttcg cggcggcggc ggtcctctac ggcgccctgg
      301 ccgcggcggt gttgtcgctc cggccccagt gggtcggcgt ggcgccgacg gacgcccgcc
      361 ggttgttttc ctgggtcggg gccgccctgc gcggtcggcc gcggaaaatt taaattcgaa
      421 ggagcgattg catggaatcc aaggacttga cctggaagga acgaatccgc cgtcggtcgc
      481 tggaggcggc ccaacgggaa caggggctga cggatctcgt ggggcgcctt cgggagatcg
      541 tcccggacat cgcggggcag gaatcctccg aggaggaatt gttcaacccc tactgggaag
      601 tcaagcgtcg gggcctgcag gccttccaat gccgggagat gttggccgcc gtggacgccc
      661 tggggcgcgc gccgggcgcg ggcctcaccg tggtggacat cggcgattcc gccgggaccc
      721 acctgcttta cctcaaggcc cttctggggc cggcccgccc ggtggaaacc gtgagcgtca
      781 acctggaccc gcgggccatc gccaaaatac aggcccgggg aatgaaggcc gttctgtgtc
      841 gagccgagga ccttcggttg gaccgccccg tggatctttt cgtgtccttt gaaatgctgg
      901 aacacctgca caaccccgcg ctctttctcc gccggttggc ttgccgggac gaccaaagcc
      961 gcctgctgct caccgtgccc taccgccggc gaagccaggt gggcctccac cacgtgcggc
     1021 ggaacgacct caaaaccgtc cacgccgagg aggagcacgt tttcgagctg agccccgggg
     1081 attggacgct tttgttccgt cacgccgggt ggcgggtggc gcggtccgcc acgtaccgcc
     1141 agtacccccg ccggtggaac cccgccgcgc ccctctgggc ctggtggtgg aaccgctcgg
     1201 atttcgaggg gttctgggcc gtcctgttgg aacgggaccg gtccttgtcc gatcgctacg
     1261 tggattggga aaaatgaacg tgccgcctcc cgccgacgct tccgcccgcc gttggctgct
     1321 ggtcaccgac gggcccccgg gtccgttccc ggaggccgaa gtcgtttttc ctctgacgga
     1381 cgatccggcg gcgttggagg ccctggccga aaaattcggc gaccgcctcg cgccgtccgg
     1441 cgagcggatt
//
LOCUS       DAOQLS010000598         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_72251_length_1449_cov_2.224534, whole genome shotgun sequence.
ACCESSION   DAOQLS010000598 DAOQLS010000000
VERSION     DAOQLS010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72251_length_1449_cov_2.224534"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK362_09840"
     CDS             complement(<1..777)
                     /locus_tag="PK362_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC75168.1"
                     /translation="MKPQMGRGLFFFRVILLWTLAFAPGPLLAADLSRPAKDSLAREA
                     AALSQSGHYPEARAKWTELLNGGPSRAEARRWRPELGRTYEAEGNFQKALAVYQEAYD
                     ADPKNVDRLVDLARLYDVVDMDDQAIRYYAEAHERDRARRDVSLALARLYKQFGRLAE
                     AKDLAVNAVHAEPRDYSGQELLAEIEEAQGALADAARRRETVVGMHPTAAGYMLLGRL
                     WAKQGAFEQADVAFSRATAAGETGFEPLFERAVLAWRQGDP"
     gene            complement(960..>1449)
                     /locus_tag="PK362_09845"
     CDS             complement(960..>1449)
                     /locus_tag="PK362_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC75169.1"
                     /translation="IRTGEGIVLREGSVALAARASATMPGIFEPVPFRHRLLIDGGVV
                     DNVPTEVARRLGADILLCLYVPEDFSRHNVTNVLTMITQALYIQGQVISEERIKRADY
                     VIRPDVSDISALELWRSKECMEAGQRAAETAWPDLKRYLAEKFLERALSPTGAAPAAG
                     TK"
BASE COUNT          252 a          495 c          441 g          261 t
ORIGIN      
        1 ggggtccccc tgacgccacg ccagaaccgc ccgctcgaag agcggttcga atcctgtctc
       61 ccccgccgcc gtcgcccggg aaaacgccac gtcggcctgc tcgaacgcgc cctgtttcgc
      121 ccagagccgt cccaacagca tgtaaccggc cgccgtgggg tgcatgccca ccacggtttc
      181 ccgccggcgc gcggcgtcgg ccagggcgcc ctgggcctct tctatttccg ccaacagctc
      241 ttggccggaa tagtcccggg gctccgcatg gaccgcgttc accgccaggt ctttcgcctc
      301 cgccaaacgg ccgaattgtt tgtagagccg cgccaaagcc aaagacacgt cccggcgcgc
      361 gcggtcccgc tcgtgggcct cggcgtagta ccggatcgct tggtcgtcca tgtccaccac
      421 gtcgtagagc cgggccaagt ccaccaagcg atcgacgttt ttgggatcgg cgtcgtaggc
      481 ttcttgatac accgccaacg ctttctgaaa gttcccctcc gcttcgtacg tgcgccccag
      541 ttcgggccgc catcgccgcg cttcggctcg ggacgggccg ccgttcagga gttcggtcca
      601 tttggcccgc gcctccggat aatggccgga ttgggaaagg gccgcggcct cccgggccaa
      661 actgtctttc gccggccggg aaaggtcggc cgcgagaagg gggccgggag cgaacgccag
      721 ggtccagaga agaatcaccc gaaagaaaaa caacccgcgc cccatttgcg gtttcattgg
      781 agccgcccag atccccggcc gtttccgccc gaaaagaaag cctgtccttc tccccttggg
      841 agaaggtgcc ccagaggggc ggatgaggga aagggttgcc cgttcgacgg aaaaatattc
      901 ccctcaccct cccctctccc aagaggagag ggaaaaaggc ttgggcaatt gcggctcatt
      961 tactttgtcc ccgcggcagg ggccgctccc gttggggaga gcgcccgttc gagaaatttt
     1021 tccgccaaat agcgtttgag gtccggccag gcggtttccg ccgcccgttg gccggcttcc
     1081 atgcattcct tcgaccgcca cagttccagg gcggaaatat cggagacgtc gggccgaatc
     1141 acgtaatcgg cccgtttgat ccgctcttcg ctgatgacct gtccctggat gtaaagggct
     1201 tgggtgatca tcgtgagcac gttggtgaca ttgtggcggc tgaaatcttc cggcacatac
     1261 aggcaaagaa ggatgtcggc gcccaaccgc cgcgccactt ccgtgggcac gttgtccacg
     1321 acgcccccgt cgattaacag ccggtgccga aacggcaccg gttcaaaaat ccccggcatg
     1381 gtggcgctgg cccgggcggc caaggcgacg ctcccttccc gaagcacgat gccttccccc
     1441 gtgcggatg
//
LOCUS       DAOQLS010000599         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_72443_length_1447_cov_2.798132, whole genome shotgun sequence.
ACCESSION   DAOQLS010000599 DAOQLS010000000
VERSION     DAOQLS010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72443_length_1447_cov_2.798132"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PK362_09850"
     CDS             <1..490
                     /locus_tag="PK362_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNC75170.1"
                     /translation="HTIENRGPIDRRLRARIGEWLFGCDLCQDACPYNARALDAQWPE
                     FRPDRGPGAWISLREVLSLRTSEAFKKKFAGTSLLRAKRAGLVRNACLVAANGGWARE
                     LMPELTDVLNRDADPVARGHAAWALRSGGVAARRVLDHAGRAETDAAVRDEIAAALEA
                     AA"
     gene            543..>1447
                     /locus_tag="PK362_09855"
     CDS             543..>1447
                     /locus_tag="PK362_09855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC75171.1"
                     /translation="MISLDQTVREEIRRRGRVPFAEFMAWALTHPRGGYYTTGPGRTG
                     RSGDFLTNVQVPLYAELLAHQFCEMWDALGSQRFTLVELGGNDGALAEGIFRELESRG
                     RHRSASLHLIEASPAARVAARKRLSRYPHVHLHASLEDLEHIAGVEGCVYSNEFFDAL
                     PVHRVRRIGNRLTELYVTEKEGRLVEDPGDPSTPQLAAALAQSGVLLADGQEGEVCLA
                     AGDVLAGVGRILARGFLWTADYGGSAAEVYAPHRVRGTLRSFSKHQLAESPLEAVGER
                     DITAHVDFTRLARLGAAEGFRPLVYA"
BASE COUNT          212 a          485 c          503 g          247 t
ORIGIN      
        1 ccacaccatc gagaaccggg gccccattga ccgccgtctg cgggcgcgga tcggggaatg
       61 gctcttcggt tgcgaccttt gccaggacgc ctgcccctac aacgcccggg ccctggatgc
      121 ccaatggccg gaatttcgcc ccgaccgggg ccccggcgcc tggatttccc tgcgggaggt
      181 tttgtccctt cgaaccagcg aagcctttaa aaagaaattc gccgggacct ccctccttcg
      241 ggccaaacgg gcgggtctcg ttcggaacgc ctgcctggtc gccgccaatg gcggctgggc
      301 gcgggaattg atgccggagt tgacggacgt gttgaatcgc gacgccgacc ccgtggcccg
      361 gggccacgcg gcctgggccc tccggtcggg cggggtcgcc gcccgccggg ttctggacca
      421 cgccggccgc gccgaaaccg acgccgcggt tcgggacgaa atcgccgcgg ccctcgaggc
      481 ggccgcgtga gacccgattt ccatcggcct ttttcgacgg tcttcgaaat cggggggggc
      541 tgttgatctc cctggatcaa accgtccggg aggaaatccg ccggcggggc cgggtgccct
      601 tcgccgaatt catggcttgg gccctcactc atccccgggg cgggtactac acgacgggtc
      661 ccggtcgaac cggccggtcc ggggatttcc tcaccaacgt tcaggtgccc ctctacgccg
      721 aactcctggc gcaccaattt tgcgagatgt gggacgccct gggttcccag cggttcacct
      781 tggtggagct ggggggcaac gacggcgccc tggccgaggg cattttccgc gaactggaaa
      841 gccgaggccg ccaccggtcg gcgtccctgc acttgatcga agcgagcccc gccgcccggg
      901 tcgcggcgcg aaaacgtctg tcccgctacc cccacgtcca cctgcacgcg tccttggagg
      961 acctggagca catcgcgggg gtggagggct gcgtttactc caacgagttt tttgacgccc
     1021 ttcccgtgca ccgggttcgc cgcatcggga atcggctgac ggagctttac gtgacggaaa
     1081 aagaggggcg tttggtggag gatcccggcg acccctccac gccgcaactg gcggcggccc
     1141 tggcccaaag cggcgtttta ttggccgacg gccaggaggg cgaagtctgc ctggcggccg
     1201 gggacgtcct ggcgggggtg gggcgaattc tggcccgggg atttttgtgg acggcggatt
     1261 acggcggttc ggcggcggag gtctacgcgc cccaccgggt ccgaggaacc ctgcggagtt
     1321 tttcaaagca ccaattggcc gaatcgcccc tggaggccgt cggcgagcgc gacatcacgg
     1381 cccacgtgga ttttacccgg ctggcgcgcc tcggagcggc ggagggattc cggcccctgg
     1441 tctacgc
//
LOCUS       DAOQLS010000600         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_72609_length_1445_cov_3.509353, whole genome shotgun sequence.
ACCESSION   DAOQLS010000600 DAOQLS010000000
VERSION     DAOQLS010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72609_length_1445_cov_3.509353"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..928)
                     /gene="sucD"
                     /locus_tag="PK362_09860"
     CDS             complement(17..928)
                     /gene="sucD"
                     /locus_tag="PK362_09860"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC75172.1"
                     /translation="MSILLNHDTKIIVQGITGSAGSFHAKGCKAYGSQVVGGVTPGKG
                     GTEVEGIPVFNTVKEAVNATGATASMIFVPPPFAADAILEAADGGVGLIVAITEGIPV
                     LDMARVKAALRGAPVRLVGPNGPGLITPGDEKRPGCKMGIMPGYIHKPGKIGIVSRSG
                     TLTYEAVWQLSQRGLGQSTVVGIGGDPVQGSTFVDMLTLFQEDRDTEAVLMIGEIGGT
                     AEEEAAHYFKSKMTKPLFAFIAGQTAPPGKRMGHAGAIVDAGGAGGKASRGTAAEKRA
                     ALEAAGVKVVDSPADMGEIVFKALRVS"
     gene            complement(925..>1445)
                     /locus_tag="PK362_09865"
     CDS             complement(925..>1445)
                     /locus_tag="PK362_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75173.1"
                     /translation="KVGLSYIRLDGNIGCMVNGAGLAMATMDIIKLHGGEPANFLDVG
                     GGATVDQVTEAFKIILSDAHVKVIFVNIFGGIMKCDVIAQGIVQAVHKTGLDRPLVVR
                     LEGNRVEEGRKILAESGLAITPASDLTEGAKKAVALAAAAARGRPSSSDGAAAARGRP
                     SSSDGAAAGRPS"
BASE COUNT          239 a          497 c          450 g          259 t
ORIGIN      
        1 gacttgacgg gcattcctag gagacccgaa gggccttgaa aacgatttcc cccatgtcgg
       61 ccggcgagtc gaccactttg acgcccgccg cctccaaagc ggcgcgcttc tcggccgccg
      121 tgccccggga ggccttgccc ccggcgccgc ccgcgtcgac gatggccccc gcgtgaccca
      181 tgcgtttgcc cgggggcgcc gtttgtccgg cgatgaaggc gaacaggggt ttggtcatct
      241 tcgatttaaa atagtgggcc gcctcttcct cggcggtccc gccgatttcg ccgatcatca
      301 acacggcctc ggtgtcgcgg tcctcctgga acagggtcag catgtcgaca aaggtcgagc
      361 cctgcaccgg gtccccgccg atcccgacga ccgtggattg gccgaggccc cgctgggaca
      421 actgccacac cgcttcgtag gtcaaggtgc ccgagcggga aacgatgccg attttccccg
      481 gtttgtgaat gtacccgggc atgatgccca ttttgcatcc gggccgcttc tcgtccccgg
      541 gcgtgatgag cccgggcccg ttggggccca ccaggcggac cggcgcgccg cgaagggccg
      601 ccttcacccg ggccatgtcc aaaacgggaa tgccttcggt gatggccacg atcaatccaa
      661 cgcccccgtc cgcggcttcc aaaatggcgt cggccgcgaa ggggggcggc acaaaaatca
      721 tggaggccgt ggcccccgtg gcgttgaccg cttctttcac agtgttgaaa accggaatgc
      781 cctccacctc ggtgccgccc ttgccggggg tcaccccgcc caccacttgg gagccgtagg
      841 ccttgcagcc cttggcgtga aaggacccgg cggagcccgt gatcccttgg acgatgattt
      901 tcgtgtcgtg gttgaggagg atgctcatga cggcctcccg gcggcggccc catccgacga
      961 actgggacgg cccctagcgg cggccgcccc atccgacgaa ctgggacggc ccctagcggc
     1021 ggccgccgcc agggcgacgg cctttttggc cccttcggtc aaatccgagg cgggggtgat
     1081 cgccaggccc gattcggcca gaatttttcg cccttcctcc acccgattgc cctccaaccg
     1141 aacgaccagg ggccggtcga ggcccgtttt atgaaccgcc tggacgatgc cctgggcgat
     1201 gacgtcgcat ttcatgatgc ccccgaaaat attgacgaag atgaccttca cgtgggcgtc
     1261 cgacaaaatg atcttgaacg cttcggtgac ttgatcgacc gtggcgccgc cgcccacgtc
     1321 caggaaattc gccggctcgc cgccgtggag cttgatgatg tccatggtgg ccatggccaa
     1381 acccgcgccg ttcaccatgc aaccgatgtt gccgtccaga cggatgtaag agaggcccac
     1441 tttgc
//
LOCUS       DAOQLS010000601         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_72931_length_1442_cov_2.170872, whole genome shotgun sequence.
ACCESSION   DAOQLS010000601 DAOQLS010000000
VERSION     DAOQLS010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72931_length_1442_cov_2.170872"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..392
                     /locus_tag="PK362_09870"
     CDS             222..392
                     /locus_tag="PK362_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75174.1"
                     /translation="MTLTGNADLIRAALRETEEYVKETLRDLESNDVDNTAVADRIRL
                     IEKSCRRLRRAA"
     gene            421..>1442
                     /locus_tag="PK362_09875"
     CDS             421..>1442
                     /locus_tag="PK362_09875"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNC75175.1"
                     /translation="MHYKTKVTALAALFATGMSAHAIEIDMSKAVQSVKVSGDFRLRQ
                     DTMETTPNATAPGATDRSRQRLRLRLGVDVALPSNFTVKTRFATGVSEQTSTNQTLDN
                     LSNQKSFWVDRAYLEWQPVTLLKIAGGKMANPLWTLYSSDILWDEDLNPEGLGESVKL
                     PPMGPVRLFANALQMAVKENKNSEHDAAGFSEQIGGEVQLPLEMRLKVGVADHYWLHV
                     SSKSANPYDLGQGSDLSATSGTAFPAKKQDGNRRIGTNGLANNFNVQEITGELSGWVV
                     GLPLSLMGTYITNVASLEKYANQPGSLANTGYQVGSRLGKAEAAKTWEVAYFYKRVQT
                     DATLADL"
BASE COUNT          381 a          390 c          379 g          292 t
ORIGIN      
        1 aaatttgcct taaggacttg taaatttatt gtaaacaccg gcacttaatc ataagcgaca
       61 aattggccaa tatttccccc cgtgacaccg agagaggtcg gggcaatccc ccaaaaaacg
      121 tcacatggcc gtcatattca ttttacttgc gcgcaatggt tttccgagtc gaatccgggt
      181 agagtgtcgg caaaaataag agagacaagg agacagaccc catgacactg acgggaaacg
      241 cggacctgat tcgggccgcc cttcgggaaa ccgaagaata cgtgaaggag acgttgaggg
      301 acttggaatc caacgacgtg gacaacacgg ccgtggcgga ccgcatccgc ctcattgaga
      361 agagttgccg ccggcttcgc cgggcggctt gaatttgaac taaatcacag gaggaatgaa
      421 atgcattaca aaacgaaggt taccgctttg gccgctcttt ttgcgacggg gatgtccgcc
      481 cacgccattg agatcgacat gtccaaggcc gttcaaagcg tcaaagtgtc gggagacttc
      541 cgactgcggc aggacaccat ggagaccacc cccaacgcca ccgcccctgg cgctacggac
      601 cgtagccgtc aacgtcttcg cctacgatta ggcgttgatg tggcgcttcc cagcaacttc
      661 accgtcaaga cgcgtttcgc gacaggcgta agcgaacaaa cgtccacaaa tcaaacactg
      721 gataatctct ccaaccaaaa aagcttctgg gtcgaccgcg cttatttaga gtggcaaccc
      781 gtgaccctct taaaaattgc gggtggtaaa atggccaatc ctctgtggac cctctattct
      841 tcggatattc tctgggacga agacttgaat ccggagggat tgggggaaag tgtgaagctt
      901 ccaccaatgg gtcctgtccg tctctttgcc aacgcattac aaatggcggt aaaagagaat
      961 aaaaattcag aacacgacgc cgcgggattt agcgaacaaa ttggtggaga ggttcagctc
     1021 ccccttgaaa tgcgcctcaa agtcggcgtg gctgaccact actggttgca cgtgagctca
     1081 aaaagcgcga atccatacga cctggggcaa ggcagtgacc tctccgccac ctccggcact
     1141 gccttcccgg ctaagaaaca ggacggcaac cgccgaattg ggacaaatgg attggccaac
     1201 aacttcaacg ttcaagaaat cacaggcgaa ttgagtggtt gggtggtcgg attgcccttg
     1261 tcactcatgg gcacttacat cacgaacgtg gcgtccttgg aaaaatatgc caaccaacct
     1321 ggctctctcg ccaacacggg gtaccaagtg ggctcccggt tgggcaaggc cgaagcggcc
     1381 aagacctggg aagtggctta cttctataaa cgtgttcaaa ctgacgccac cttggccgat
     1441 ct
//
LOCUS       DAOQLS010000602         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_73015_length_1441_cov_2.602453, whole genome shotgun sequence.
ACCESSION   DAOQLS010000602 DAOQLS010000000
VERSION     DAOQLS010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73015_length_1441_cov_2.602453"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK362_09880"
     CDS             <1..561
                     /locus_tag="PK362_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012069591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNC75176.1"
                     /translation="QHVQVADTLMRQAVREAGAGQNVMIVIDSLTRLARVHNAERHSS
                     GRTLSGGMDARAMEIPRRIFGAARKIENGGSLTILATVLVETGSRMDDVIFEEFKGTG
                     NMEIILSREISTRRIFPAIDIAKSGTRKEELLLGPEELGAVRKLRRVLAKMTPVDAAQ
                     QLVTELQSYPTNKEFLAAVDKFASDK"
     gene            581..1096
                     /locus_tag="PK362_09885"
     CDS             581..1096
                     /locus_tag="PK362_09885"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNC75177.1"
                     /translation="MVGLALAACIAGGKSDSRFKTYQNTKYHYTIDYPAAWTAEGTAA
                     DIAESVEVTFFKVNEKAGAVKGMTLITVHVYNNPRGRPPEQWYAQDAAKESDGLYPYP
                     AKIVASAETTTVNGHPAYRVGTTDEAESFDYFVGAGTAIYRLSFEKFAGDEATPKELF
                     ERSLKSFRISQ"
BASE COUNT          323 a          396 c          440 g          282 t
ORIGIN      
        1 cagcacgtgc aggtggcgga caccctgatg cgccaggcgg tgcgggaagc gggcgcgggc
       61 cagaacgtga tgatcgtgat cgattccctg acccgcctgg cgcgggtgca caacgcggag
      121 cggcattcca gcgggcggac gctctcgggc gggatggacg cgcgggccat ggaaatcccc
      181 cggcggatct tcggcgcggc gcggaaaatt gaaaacggcg gctccctgac gatattggcc
      241 acggtgctgg tggagaccgg aagccgcatg gacgacgtga ttttcgagga gttcaaaggc
      301 acggggaaca tggagatcat tttatcccgg gagatatcga cccggcggat tttcccggcc
      361 atcgacatcg ccaaaagcgg cacgcgcaaa gaggaattgc tgttgggccc cgaggagttg
      421 ggcgcagtgc ggaaattgcg ccgcgtgctg gccaagatga ccccggtgga cgcggcccag
      481 cagttggtga cggagctgca gtcctacccg acaaacaaag agtttttggc cgcggtggac
      541 aagtttgcct cggataaata gtttctcccc gttcgttccg ttggttggat tggccctggc
      601 ggcttgtatc gccggcggga agtccgacag ccggtttaaa acctaccaaa acaccaaata
      661 ccactacacg atcgactatc ccgcggcctg gaccgccgag ggaacggccg cggacatcgc
      721 cgagagcgtg gaagtcacct ttttcaaagt caacgaaaag gccggggccg tcaaaggcat
      781 gacgctgatc accgtgcacg tctacaacaa cccccggggg cggccgccgg agcaatggta
      841 tgcccaagac gctgccaaag agagcgatgg gctttacccc tacccggcca aaatcgtggc
      901 ttcggcggag acgacgacgg tcaacggcca tccggcttat cgggtgggga cgacggacga
      961 agcggagagt ttcgactatt tcgtgggggc cgggacggcc atctatcgat tgagttttga
     1021 gaagtttgcg ggggacgagg caacgcccaa ggaattgttt gaacgctcgc taaaatcttt
     1081 tcgaatttcg cagtaatttc ctgcctttta ataaaaccaa tttcatactt cgtgggggcc
     1141 gggacggcca tctatcgatt gagttttgag aagtttgcgg gggacgaggc aacgcccaag
     1201 gaattgtttg aacgctcgct gaaatctttt cgaatatccc agtagtttcc tgccttttaa
     1261 taaaaccaag ttcatcattc gcgggccttc cggttctgca cccaattttt ttcccaacct
     1321 tgaaatcgag aatcagaatt tggcgatcaa actttaaatc caagtcgtgg gcctccccgg
     1381 cccacgcccg gccaaggcca tttttggacg cgcaaaaatg gccctggacc caaaaacgcg
     1441 c
//
LOCUS       DAOQLS010000603         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_73198_length_1439_cov_3.606936, whole genome shotgun sequence.
ACCESSION   DAOQLS010000603 DAOQLS010000000
VERSION     DAOQLS010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73198_length_1439_cov_3.606936"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PK362_09890"
     CDS             <1..434
                     /locus_tag="PK362_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC75178.1"
                     /translation="DSGARGATVTFVERGIGDVLVAWENEAFLAVNEGGKGRFEIVVP
                     SVSILAEPPVALVDKVVDRHGTRKTAEAYLKFLYTDEAQEIVARNYYRPRSAAVAAKH
                     TGDFPKLRLFTIDELFGGWQKAQKVHFADGGVFDQIYQPGR"
     gene            471..1298
                     /gene="cysT"
                     /locus_tag="PK362_09895"
     CDS             471..1298
                     /gene="cysT"
                     /locus_tag="PK362_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNC75179.1"
                     /translation="MLKSAGVLPGFGLSLGYTVFYLSLVVLIPLSTLFAKSATLGPAA
                     FWDAVTTPRVLAAFRLSFGASALAAGINSVFGLLVAWVLVRYSFPGRRFVDAVVDMPF
                     ALPTAVAGIALTALYAPNGWVGAPLARFGIQAAFAPMGVVIALIFIGLPFVVRTLQPV
                     LETMESDMEEAAMTLGASRWQTIRRVVFPPLVPALLTGFALSFARAVGEYGSVAFISG
                     NMPMKTEIAPLMIMTKLEQYDYAGATAVAVVMLFLSFGVLFAVNALQAWSRGRVGEK"
BASE COUNT          207 a          453 c          464 g          315 t
ORIGIN      
        1 tggattccgg cgcccggggg gcgaccgtga cctttgtgga gcgggggatc ggcgacgtcc
       61 tggtggcctg ggagaacgaa gcctttctgg ccgtcaacga agggggaaag ggccgctttg
      121 aaatcgttgt gccctcggtc agcattctgg ccgaaccgcc cgtcgccctg gtggacaagg
      181 tggtcgaccg ccacggaacg cggaagacgg ccgaggcgta cttgaaattc ctctacaccg
      241 acgaagccca ggagatcgtt gcgcggaact attatcgtcc ccgctcggcg gccgtggccg
      301 cgaagcacac gggggacttt ccgaagctcc gtcttttcac catcgacgaa ctgttcggcg
      361 gatggcaaaa ggcccagaag gtccatttcg ccgacggcgg ggtgttcgat caaatttacc
      421 aaccgggccg gtgagccgtc cggatagggt aatatagcgc cgtgaaaaaa atgttgaaat
      481 cggcgggggt gttgccgggg ttcgggttgt ccttgggcta caccgttttc tatctgagtc
      541 tcgtcgtgtt gatccccctc tcgaccctgt tcgccaagtc ggcgaccctg gggcccgccg
      601 ccttttggga cgccgtcacg accccccggg tgttggcggc cttccgtttg agtttcggcg
      661 cttcggccct ggcggcggga atcaacagcg ttttcggcct gttggtggct tgggtgttgg
      721 tccgctattc ctttccgggc cgccgattcg tcgatgccgt ggtcgacatg cccttcgccc
      781 tgccgacggc cgtggcgggc atcgccttga cggccctcta cgcccccaac ggatgggtcg
      841 gcgcgcccct ggcccgattc ggcattcagg cggccttcgc gcccatgggc gtcgtgatcg
      901 cgctcatttt catcggtctg ccctttgtcg tgcgcacgct ccaacccgtt ttggaaacca
      961 tggagtcgga tatggaagaa gccgccatga cgttgggggc ttcccgttgg caaacgatcc
     1021 gccgggtggt gtttccgccc ctggtgccgg cgctcctcac cggatttgcc ctctccttcg
     1081 cccgggccgt cggcgaatac ggttccgtgg cctttatttc cggcaacatg cccatgaaaa
     1141 cggaaatcgc gcccctcatg atcatgacca agttggagca gtacgattac gccggggcca
     1201 ccgccgtggc cgtcgtgatg ctcttcctgt cttttggcgt gctcttcgcc gtcaacgcgc
     1261 ttcaggcctg gagccgcggc cgggtggggg aaaaataaat ggccggcgcg acgtccaccc
     1321 tggttcggtc cgccgccggg cccgtccacc gtcccctcac ggagccgcgg tgggtccaag
     1381 tcctgttgat cggcgtcgcg atggtgtttc tggggttttt cctgttgttg cccaccctg
//
LOCUS       DAOQLS010000604         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_73386_length_1437_cov_4.478292, whole genome shotgun sequence.
ACCESSION   DAOQLS010000604 DAOQLS010000000
VERSION     DAOQLS010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73386_length_1437_cov_4.478292"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PK362_09900"
     CDS             <1..217
                     /locus_tag="PK362_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75180.1"
                     /translation="FGGGSEYRWTPGPRWTLAGRLGYSNRARDIRGVTGVSGGLGVLY
                     KKIQIDYALVPYGDVGLTHLVSLTTYF"
     gene            282..>1437
                     /locus_tag="PK362_09905"
     CDS             282..>1437
                     /locus_tag="PK362_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNC75181.1"
                     /translation="MGSEKDNNLKSTNGDGSSRRILVVSRVGCVGDLCRQLQTEGHHV
                     RYFIESKGDKDVSDGFVEKTDDWKALRAWADLVIFDDSDFGRDAEALRRDGKPVVGGT
                     VYTDRLEDDRDFGQQEMKAAGMTILPNWTFTSFEEAVAFVKKNPDRYVVKPSGSAQNE
                     KVLSYVGQEEDGQDVVAILERYKKGWARQIRSFQVQKFAAGVEVAIGAFFNGKEFLSP
                     ACVNFEHKKMFNEDIGPATGEMGTSMFWMTDSALYRETIAKMASRLAAVGFVGYFDIN
                     CIATPRAIYPLECTPRFGYPTLSIQMEGVTSRWGDLFYGLATGQTPVLKTKKGYQVGV
                     VIAVPPFPFEDATAFRKYSEGAVVIFKKPVTEGLYPGDIKLVDGDWVLAGQ"
BASE COUNT          288 a          441 c          441 g          267 t
ORIGIN      
        1 gttcgggggc ggctccgaat accgttggac ccccggcccc cgttggactc tggccggccg
       61 cctcggctac agcaaccggg cccgggacat tcggggggtc accggcgtca gcggcggcct
      121 cggcgtcctc tacaaaaaaa tccaaatcga ctacgccctc gtgccctacg gggacgtggg
      181 gctcacgcac ctcgtttccc tcaccacgta tttctaaatc gcgcgtccgc gcgtaaattt
      241 cccttgtgaa ttgacccgtt ccgggatacc ctctagggga gatgggttcc gaaaaagaca
      301 acaatttaaa atcaaccaac ggcgacgggt cctcccggcg gattttggtc gtgtcccggg
      361 tgggctgcgt gggggacctt tgccgccagc ttcaaaccga gggtcaccac gtccgctact
      421 ttattgaaag caagggcgac aaggacgtct ccgacggctt cgtcgagaaa accgacgatt
      481 ggaaggccct tcgcgcctgg gccgacctcg tgatcttcga cgattcggat ttcggtcggg
      541 acgcggaagc cctgcgccgg gacggcaagc ccgtggtggg cggcaccgtc tacaccgacc
      601 gactggagga cgatcgggat ttcggccagc aggaaatgaa agccgccggc atgaccatcc
      661 tccccaactg gacgttcacc tcctttgaag aagccgtggc cttcgttaaa aaaaaccccg
      721 accggtacgt ggtcaaaccc tccggcagcg cccagaacga aaaagtgctt tcttacgtgg
      781 gccaggagga ggacggccag gacgtcgtcg ccattttgga gcggtacaag aaggggtggg
      841 cccgtcagat ccgctccttc caggtccaga aattcgccgc cggggtcgag gtcgccatcg
      901 gcgcgttttt caacgggaag gaattcctgt ccccggcctg cgtcaacttc gaacacaaaa
      961 aaatgttcaa cgaggacatc ggtcccgcca ccggcgaaat gggcacgtcc atgttttgga
     1021 tgacggacag cgccctttac cgggagacca tcgccaaaat ggcgtcccgc ttggcggcgg
     1081 tcggtttcgt gggctatttc gacatcaatt gcatcgccac gccccgggcg atttacccct
     1141 tggaatgcac gccccggttc ggctacccga cgctcagcat ccaaatggaa ggggtgacca
     1201 gccggtgggg ggatttgttc tacgggctgg ccacgggcca gacgccggtc ctcaagacca
     1261 aaaaaggcta tcaggtgggg gtggtgatcg ccgtgccgcc ctttcccttc gaggacgcga
     1321 cggcctttcg caaatattcc gaaggcgccg tggtaatttt caaaaagccc gtcacggagg
     1381 gcctctaccc cggggacatc aagctggtgg acggggactg ggtgctggcg gggcaat
//
LOCUS       DAOQLS010000605         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_73489_length_1436_cov_5.889935, whole genome shotgun sequence.
ACCESSION   DAOQLS010000605 DAOQLS010000000
VERSION     DAOQLS010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73489_length_1436_cov_5.889935"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /gene="metF"
                     /locus_tag="PK362_09910"
     CDS             complement(<1..887)
                     /gene="metF"
                     /locus_tag="PK362_09910"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNC75182.1"
                     /translation="MKIPHILAQKRPAFSFEFFPPKTDEDAAHLLATARQLKELDPAF
                     ISVTWGAGGSTRRKTLDIVSAVKKDLGVETMAHLTCVGASREDIDGVLAEIRERGIKN
                     VLALRGDPPKGSTAFVTHPGGFAHANELVAHIKRFGDFSVGVAGYPEKHVEAPSLEAD
                     LDHLKRKVDAGADFIITQLFFGNDDFLRFRDKAAARGIALPILPGLMPVTNVGQLKRF
                     TTLCGAKIPGDLLQRLEAVQDNAEAVIRLGVAHAVAQGRRLLDAGVPGIHFYTLNRSR
                     STAPSAQERQRMLLRAMAH"
     gene            948..1271
                     /locus_tag="PK362_09915"
     CDS             948..1271
                     /locus_tag="PK362_09915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNC75183.1"
                     /translation="MRPLTVVAQARAKKGSEKFLEDALRRVARESQREEGCLMYVVHR
                     DAGDPAFFMTVERWRSQSDIDRHMASSHIGELLRRIPEWVAGAPTITVLEALVDGLPE
                     KGFPN"
BASE COUNT          262 a          437 c          485 g          252 t
ORIGIN      
        1 cagtgcgcca tcgcccgcag cagcatgcgc tggcgctcct gcgccgaggg ggcggtggat
       61 cgggagcgat tgagggtgta aaaatggatt cccggcacgc cggcgtccag caatcggcgg
      121 ccctgggcaa cggcgtgggc gacgcccagg cggatgaccg cttcggcgtt gtcctggacc
      181 gcctccaacc gctgaaggag gtcccccggg atcttggcgc cgcaaagagt ggtgaaccgt
      241 ttcaactgac cgacgttcgt cacgggcatg aggcccggca gaatcggcaa agcgatcccg
      301 cgggcggcgg ctttatcgcg aaaacgaagg aaatcgtcgt tgccgaaaaa aagttgggtg
      361 atgatgaaat ccgccccggc gtcgactttt cgtttgagat ggtccagatc ggcttccaag
      421 gacggggcct ccacgtgctt ttcgggatac cccgccacgc cgacgctgaa atccccgaac
      481 cgcttgatgt gcgccaccaa ctcgttggcg tgggcgaacc cccccgggtg ggtcacaaaa
      541 gccgtggacc ccttgggcgg atcgccccga agagccagga cgtttttgat gccccgttcg
      601 cggatctcgg ccaggacgcc gtcgatgtcc tcccgggaag cccccacgca ggtcaggtgg
      661 gccatcgttt ccacgcccaa atcttttttc acggcgctca cgatgtccaa ggtcttccga
      721 cgggtgctgc ccccggcccc ccaggtcacg gagatgaaag ccgggtccaa ttccttcaat
      781 tgccgggcgg tggccagcaa atgggcggcg tcctcgtccg ttttgggagg aaaaaattcg
      841 aaggaaaagg cggggcgctt ctgagcgagg atgtggggga tcttcacggc ccgcaaattc
      901 tatctttttt gcttaaatca cttgtcgttt cccaggaagg aggcgccgtg cgcccattga
      961 ccgtggtggc ccaggcccgg gccaaaaaag gatcggaaaa atttttggag gacgccctcc
     1021 ggcgggtcgc ccgggagagc cagcgcgagg aggggtgttt gatgtacgtc gtccatcgcg
     1081 acgcggggga cccggcgttt ttcatgacgg tggaacgttg gcgaagccag tcggacatcg
     1141 accggcacat ggcgtcctcc cacatcggcg agcttttgcg ccgaatcccc gaatgggtgg
     1201 cgggcgctcc cacgatcacg gttttggagg cccttgtcga cggccttccg gaaaagggat
     1261 tcccgaactg acatggatcc gaaaggcaaa acggcgttga tcacgggcgc ggcccgggtg
     1321 ggcggcgccg tggccgaggc gctggccgca cgcgggtgcc gggtggccct gtcgtatcgc
     1381 tcttcccgaa acgcggcgga aaaaaccgcc cgggccatcc agcggcgcgg cgggga
//
LOCUS       DAOQLS010000606         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_73632_length_1435_cov_3.089130, whole genome shotgun sequence.
ACCESSION   DAOQLS010000606 DAOQLS010000000
VERSION     DAOQLS010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73632_length_1435_cov_3.089130"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PK362_09920"
     CDS             complement(<1..1150)
                     /locus_tag="PK362_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC75184.1"
                     /translation="MKKWILVLWFSAAPLWGAESEGVFPTVDELLNRVEQFNPELRAA
                     RGEAEAAAARADRAGVWPNPVLGFSKEKIPAGDRMTHWRGEQEIPFPGKPTQERRMGR
                     HEAASAEARARLKTLEFRTEARDLAARLRRSEAVTSLIGQEIESLDALVASLRARLSA
                     GRGGSAGMGGAASGSPAADLFSLEAERGRMENMLREERQEGRAARLRLNQLLDRSLDG
                     PIVVSAPPALLPPAQPLEALLETSKTESPMLALARHERGHARAQRTQAALAWMPDLAV
                     MVDEQKMDTGESGRELGVSLSVPLWISGPGADNREARRHRAAADDAARAMTVEVERQL
                     RTEHAEVLARLESARAYERSIRPAAQSALDLVRRQYESGRGEFARVLVA"
     gene            complement(1245..>1435)
                     /locus_tag="PK362_09925"
     CDS             complement(1245..>1435)
                     /locus_tag="PK362_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75185.1"
                     /translation="RSFTPAPVPAAPAVVWNGRVETVPVSPFRSVNFTVESSVRPPPG
                     QSAGFLLAFSLRAPPTAA"
BASE COUNT          248 a          487 c          482 g          218 t
ORIGIN      
        1 gggcgaccag cacccgggcg aattcccccc gcccgctttc gtattgccgt cgcacaagat
       61 ccagggcgct ttgggccgcg ggacggatgg accgctcgta ggcccgggcc gattccaatc
      121 gggccaggac ttcggcgtgt tccgtgcgca gttggcgttc gacttcgacg gtcatggccc
      181 gggcggcgtc gtcggccgcg gcgcgatgcc ggcgggcctc ccggttgtcg gccccgggcc
      241 cggagatcca cagaggcacc gagagggaaa cacccaattc ccgtcccgat tcccccgtgt
      301 ccattttttg ctcgtccacc atgacggcca ggtcgggcat ccaggccagg gcggcttggg
      361 ttcgttgggc ccgggcgtgg ccccgttcgt gtcgggccag ggccaacatg ggactttcgg
      421 ttttcgacgt ttccaggagc gcctccaggg gttgggcggg cggcaacagc gcgggcggag
      481 ccgaaaccac gatcggccca tccagggatc gatccaacag ttggttcagg cgcaggcggg
      541 ccgcccggcc ctcctgtcgc tcctcccgaa gcatgttctc catgcgtccc cgttccgcct
      601 ccagggaaaa caggtccgcc gccggagaac cggaggccgc cccccccatc ccggcggatc
      661 cgccccgacc cgccgaaagg cgggcccgca gagaggccac cagggcgtcc agggattcga
      721 tttcctggcc gatcagggac gtcaccgctt ccgaccggcg aaggcgggcc gccaaatccc
      781 gggcttcggt tcggaattcc aaggttttca accgcgcccg ggcttccgcc gaggcggcct
      841 cgtggcggcc catgcggcgc tcctgggtgg gtttccccgg aaaggggatt tcttgctcgc
      901 cccgccagtg ggtcattcgg tcgccggcgg ggattttctc ctttgaaaaa ccgaggacgg
      961 ggttgggcca cacccccgcg cggtccgccc gggccgccgc cgcttcggcc tctccccggg
     1021 ccgcccgaag ttcggggttg aattgctcca cccgattcaa caattcatcc accgtcggaa
     1081 aaacgccctc cgactccgcg ccccacaacg gcgcggcgga aaaccataaa accaaaatcc
     1141 attttttcat tgaacgctcc aaaccgatcg aagccgatcc cggaccgact gcccggagcg
     1201 agggggatta aacgatgaaa aggaaagaga cccgacgccc ggtgttaggc ggcggtggga
     1261 ggagcgcgga gggaaaaggc gagcaaaaac ccggcggatt ggccgggcgg cggacggacg
     1321 gaagattcaa cggtgaaatt aaccgagcga aaaggagaaa cgggaacggt ttccacccgg
     1381 ccgttccaca ccaccgcggg cgcggcgggg acgggggccg gagtgaagga ccgct
//
LOCUS       DAOQLS010000607         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_73678_length_1435_cov_2.125362, whole genome shotgun sequence.
ACCESSION   DAOQLS010000607 DAOQLS010000000
VERSION     DAOQLS010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73678_length_1435_cov_2.125362"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK362_09930"
     CDS             <1..419
                     /locus_tag="PK362_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75186.1"
                     /translation="MKRSTRRVDRGVEKRLADFEPYDGENGKLVERAAAGCAIHDRSI
                     LISEVVAGIAEESKPEAKTVKLPPLSEVMILDRPSTVGEFEAFVKKLTVVPVTQVNGE
                     SPLGQYTTYEAVDAQGARYRISVEGKRKTVSGTLGK"
     gene            463..>1435
                     /locus_tag="PK362_09935"
     CDS             463..>1435
                     /locus_tag="PK362_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC75187.1"
                     /translation="MILLTGATGYVGGRLLKSLGKAEREVRCLARRPEALASRLAPGV
                     EVVAGDVLDPAALEKALAGVEAAYYLIHSMGNAVDFEEQDRRQARLFGEAAKRAGVRR
                     ILYLGGLGEEGPGLSPHLRSRQEVGRLLRESGVPVLEFRASIVLGSGSLSFEMIRSLV
                     ERLPVMVTPRWVTVEAQPIGIEDLLAYLMAGLTVPLTQSRIYEIGGADRMSYADLMRE
                     YARQRGLKRWMIPVPVLTPWLSSLWLGLVTPLFARIGRKLIESIRHPTVVRDDAARRD
                     FDVRPGGVREAMALALRNEERQFAETRWSDAFSSLGGTEVFHTRLGNR"
BASE COUNT          241 a          362 c          555 g          277 t
ORIGIN      
        1 tcatgaagcg gtccaccagg agggttgacc gcggggtgga gaagcggttg gcggattttg
       61 agccctacga cggggagaac ggaaaattgg ttgaaagggc ggcggcgggt tgcgcgatcc
      121 acgaccggtc gattttgatt tcggaagtgg tggcgggaat agcggaagag tcaaagccgg
      181 aagccaagac cgtgaaactg ccgccattgt cggaagtgat gatcctggac aggccttcga
      241 cggttgggga atttgaggcc tttgtgaaga aattgacggt cgttccggta acccaggtga
      301 acggggaaag ccccctggga caatacacga cctacgaggc ggtggacgcc cagggcgcgc
      361 gttatcgcat ctccgtggaa gggaaacgga agaccgtgag cgggaccctg ggcaagtgaa
      421 cggtcgaaag cttcggggcc agtcgatttt cgggacgacc atttgatcct actgacgggg
      481 gccaccgggt atgtgggggg gcgattgtta aagtcccttg gcaaggccga gcgggaggtt
      541 cggtgtttgg cgcggcggcc ggaggccctg gcttcgcgac ttgcgcccgg ggtggaggtg
      601 gtggccgggg acgttctgga cccggcggcg ttggagaagg ccctggcggg ggtggaggcg
      661 gcctattatt tgatccactc catgggaaac gcggtggatt ttgaggagca ggaccgtcgg
      721 caggcgcggc ttttcggcga ggcggccaag cgggcggggg tgcggcggat cctctacctg
      781 ggcgggttgg gggaagaggg ccccgggctg tcgccccatt tgcggagccg gcaggaagtg
      841 gggcgcctgt tgcgggagtc gggcgtgccg gtgttggagt ttcgggcgtc gatcgttttg
      901 ggatcgggca gtttgtcttt tgaaatgatc cgctcgctgg tggagcggtt gccggtcatg
      961 gtcacgcccc gctgggtgac ggtggaggcc caacccatcg gcattgaaga cctgttggcc
     1021 tatttgatgg ccggactgac cgtgcccctg acccaaagcc ggatttacga gatcggcggg
     1081 gcggaccgaa tgtcctacgc ggatctcatg cgcgaatacg cccgtcagcg ggggcttaaa
     1141 cgatggatga tcccggtgcc ggtcttgacg ccctggctct ccagcctgtg gttggggctt
     1201 gtgacgccgc tctttgcccg gattggccgg aagctgattg aaagcattcg ccatccgacg
     1261 gtggtgcggg acgacgcggc tcggcgggac tttgatgtgc gccccggggg cgtgcgggag
     1321 gccatggccc tggccctgcg aaacgaagag cgccagtttg cggagacccg gtggtccgac
     1381 gctttctctt ccttgggggg tacggaagtt ttccacaccc ggttggggaa ccggc
//
LOCUS       DAOQLS010000608         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_74034_length_1431_cov_4.452762, whole genome shotgun sequence.
ACCESSION   DAOQLS010000608 DAOQLS010000000
VERSION     DAOQLS010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74034_length_1431_cov_4.452762"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1071
                     /locus_tag="PK362_09940"
     CDS             118..1071
                     /locus_tag="PK362_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC75188.1"
                     /translation="MKKILVTGAAGFIGAWTAKALRERGHTVVGVDSFSPYYDVTLKR
                     ARVRALLPDVEIVEADIADRRALEPIFARHRFDQICHLAAQAGVRHSLKDPYAYESAN
                     GLGTLNLLEFARHGGRPAFVFASSSSVYGGNTKIPFSVDDPVDRPVSLYAATKRSNEL
                     TAHAYHHLFGLRCTALRFFTVYGPWGRPDMALFGFTKNILEGKPIDVYNHGDMRRDFT
                     FITDIVDGVVAALEREYDYAIFNLGHSDPVPLTRFIELIEKAVGRPAKKNLLPLQPGD
                     VPATWADIAKSTERLGFRPRVSIEEGIRQFVDWYRTYYQTP"
     gene            1173..>1431
                     /locus_tag="PK362_09945"
     CDS             1173..>1431
                     /locus_tag="PK362_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75189.1"
                     /translation="MKSKFAAFVAVCYAATNVLWATAPEAAFWSERRRALRRERPTLL
                     ASATPVGADPAVALGGLPLPAALPPEAVARLPAEFQERHAGL"
BASE COUNT          256 a          499 c          413 g          263 t
ORIGIN      
        1 gtcgcctttc gcggcgcggg tccaggaagt caccctcgcc atcaacgtgc tccccccggg
       61 ggcctacgac gtggcgcagg acgaacgcac gggagaattg acggtgaaag cgcgcaaatg
      121 aaaaaaattc ttgtgaccgg cgccgccgga ttcatcggcg cctggaccgc caaagccctt
      181 cgcgaacggg gccacaccgt cgtgggggtg gacagtttct cgccctacta cgacgtgacc
      241 ctgaagcgcg cccgggtccg ggccctgttg ccggacgtgg aaatcgtcga agcggacatc
      301 gcggaccgcc gcgccctgga gccgattttc gcccgccacc ggttcgacca aatttgccat
      361 ctggcggccc aggccggcgt ccgccattcg ctcaaagatc cctacgccta cgaaagcgcc
      421 aacggcctgg gcacgctcaa cctgttggag ttcgcgcggc acggcggacg tcccgcgttc
      481 gtgttcgcct cctcctcctc ggtctacggg ggaaacacga aaatcccctt ttccgtggac
      541 gacccggtgg atcggcccgt ttccctctac gccgccacga agcggtccaa cgaactcacg
      601 gcccacgcct atcaccatct gttcggtttg cggtgcaccg cccttcggtt cttcacggtc
      661 tacggtccct ggggccgccc cgacatggcg ctcttcgggt tcacgaagaa catcctggag
      721 ggaaaaccca tcgacgtgta caatcacggc gacatgcgcc gggattttac gtttatcacc
      781 gacatcgtcg acggggtcgt cgccgccctg gaacgggaat acgactacgc gatcttcaac
      841 ctcggccact ccgatcccgt gcccctgact cgatttatcg agctgatcga aaaggcggtg
      901 ggccgtccgg cgaaaaaaaa tcttctgccc ctccagcccg gggacgttcc ggccacctgg
      961 gccgacatcg ccaagtccac ggaacgcttg ggttttcggc cccgggtttc catcgaagag
     1021 gggatccgcc aatttgtgga ttggtatcga acctattacc agacccccta gcttttacaa
     1081 caacgttaca tactgttaaa gcgctctccc ccgtcgaacc cctagaatca tctcaggaca
     1141 tggaccgcct gggatcgttt ccgaaaaacg ccttgaagtc gaaatttgcc gcgttcgtgg
     1201 cggtgtgcta cgccgccacg aacgtgctgt gggccaccgc gcccgaagcg gctttctggt
     1261 ccgaacgacg ccgggctctt cgacgggaac ggccgacgct cctggcgtcg gcgacgcccg
     1321 tcggggccga tccggcggtc gcgctcgggg gactccccct tcccgccgcc cttcctccgg
     1381 aagccgtcgc ccgacttccg gccgaattcc aagagcgcca cgccggactg t
//
LOCUS       DAOQLS010000609         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_74082_length_1431_cov_2.550872, whole genome shotgun sequence.
ACCESSION   DAOQLS010000609 DAOQLS010000000
VERSION     DAOQLS010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74082_length_1431_cov_2.550872"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1162)
                     /locus_tag="PK362_09950"
     CDS             complement(167..1162)
                     /locus_tag="PK362_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC75190.1"
                     /translation="MARPLRIEFPGAWYHVMNRGAGRKAIFLNDGDRVQFLKVVDDVV
                     DTYRLEVHAYCLMNTHYHLLVRTPRGNLARAMRHLNGVYTQRFNRARRLDGPLFRGRY
                     KAILIDADSHLAQVSRYIHLNPVEAKIVRDPADCRWSSYRYFVGKESAPRWLNTTWTL
                     GLFGKTDKAAGLYRDFLMQGVDEKTKQFYGKRRQEPILGGDDFREKVGGGAAGSNPDK
                     EIAERKYLQKRLTVEEIIEFVAEGFTTTPEALLKDGRGRRGEGLPRIVGMALSRRPGG
                     HSLGEIGRVFGSSYSGVSVAERRLAEKISKDGDLAERIRAIVSSAFSGLEYKVKT"
     gene            complement(1310..>1431)
                     /locus_tag="PK362_09955"
     CDS             complement(1310..>1431)
                     /locus_tag="PK362_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNC75191.1"
                     /translation="ELVSIRIDNDVLDWFRHQGRKYQTRINAVLRSYVDVHSH"
BASE COUNT          328 a          406 c          337 g          360 t
ORIGIN      
        1 catggacaat cgtaatcgag aaatcacctt ccactgtttt tgcgagtcct caaccgggat
       61 tcccccttta gacagatgcg ggacacgccg taatcgaacg gtccgatgat ctgggcggtt
      121 ctttcgtaac gaaatggaaa ggaaaggaag gaaaggtcga aaggggtcaa gtcttgactt
      181 tgtattctaa tccgctaaaa gccgagctga cgatggctct tattctctca gcgaggtctc
      241 cgtccttgct tattttttcc gccagccgcc gttctgccac cgataccccc gaataggatg
      301 acccaaatac cctccctatt tctccaaggg agtgcccccc cggccgccga gataacgcca
      361 ttccaacgat tcgcggcaat ccctcccccc ggcggcctcg gccatccttg agtagggcct
      421 caggtgttgt cgtaaacccc tccgccacaa attcaataat ttcttccacc gttaatcgtt
      481 tttggagata cttcctttcg gcaatttcct tgtccgggtt tgaaccagcg gcgcccccac
      541 ccaccttttc ccggaagtcg tcccctccca ggatcggctc ttgtcgcctt ttcccgtaaa
      601 attgcttggt tttctcatcc actccctgca taaggaaatc tctatacagc ccggccgcct
      661 tatctgtttt tccaaacagt cccaatgtcc acgtcgtgtt taaccatcgt ggggcggatt
      721 ccttgcccac gaaataccga tagctggacc atcgacaatc cgctggatct ctgacgatct
      781 tcgcttcaac cggattgaga tgaatatacc ggctcacttg ggcaaggtgg gagtccgcgt
      841 caatgaggat ggccttgtat cgaccacgaa aaagggggcc atccagacgt cgggcacggt
      901 taaagcgctg agtatacacc ccattcaaat gccgcatggc tcgggccaaa tttcctcgcg
      961 gggtcctgac cagaagatga taatgtgtat tcatgaggca ataggcgtgg acttccaacc
     1021 ggtaagtgtc gacaacatca tcgactacct taagaaattg cacccggtca ccgtcattca
     1081 aaaagatggc tttgcggccc gcgccgcgat tcataacgtg gtaccatgcg ccaggaaatt
     1141 caattcggag aggtcgggcc atgcgagcag tttagcgact aaagagtatt aagtcaagac
     1201 ttgacccctt tattccttta tttttattat cagcgaaccg gcgttacccg gattgccggc
     1261 tgaaagccgg cagggtgacg tcctgttgag cgactggttg ggctcttttt cagtgggaat
     1321 gcacatcgac gtaagaccgg agtacggcgt taattcgagt ttggtatttt ctcccttggt
     1381 gcctaaacca gtccaaaaca tcattgtcga tcctaatcga aaccaattcc t
//
LOCUS       DAOQLS010000610         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_74531_length_1426_cov_4.721371, whole genome shotgun sequence.
ACCESSION   DAOQLS010000610 DAOQLS010000000
VERSION     DAOQLS010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74531_length_1426_cov_4.721371"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PK362_09960"
     CDS             complement(<1..124)
                     /locus_tag="PK362_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75192.1"
                     /translation="MSQTSTKDEKFKALQLALSTIEKQHGKEAIMKLGEKSAKVK"
     gene            complement(253..750)
                     /locus_tag="PK362_09965"
     CDS             complement(253..750)
                     /locus_tag="PK362_09965"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNC75193.1"
                     /translation="MTESPGRPWTQWAVLSVATVGFLGWAPYHLVPHKKWKGGGLLGT
                     LAGAALLWAAPTRGPLLWALFGIVLLVSIVTSHWAEKWLSLDDPRIVIDEVAGVWTAC
                     LLLPRLPAPFLLGAFFFRVFDVFKGPWGRAVSRLPGGWGVVADDLVAGVIAFFLTKGI
                     TAAFL"
     gene            complement(747..>1426)
                     /gene="pgsA"
                     /locus_tag="PK362_09970"
     CDS             complement(747..>1426)
                     /gene="pgsA"
                     /locus_tag="PK362_09970"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNC75194.1"
                     /translation="LRRLGAGPAETPRPGGSVPMNLPNKLTVARLASVPFFLLCVSWD
                     NVYARLGALGIFIAAGITDLVDGHIARTRNMVTPLGVFLDPLADKLIITAAFISFIEL
                     RDLHIPAWMIVAIVGREFVLTGLRAVAASQGQIMAADDGGKFKTSIQNTAIIAILVAL
                     VVASGLERFGGHSIENLYALGGWRGDAARVLDWVPFWMAFGATLFSLVTGVSYLRKHW
                     ALLKEYP"
BASE COUNT          295 a          418 c          478 g          235 t
ORIGIN      
        1 ccttcacttt ggccgatttt tcccccaact tcatgatggc ttctttcccg tgctgtttct
       61 caatggtgga aagggccagt tgcagggctt taaatttctc gtctttggtg ctggtttggg
      121 acatttggat cctccgatgg attcgaaaga agatcgtaat acatcgtttg cgggggtgtc
      181 aacaaaaact attcgtttgt atagtgatcg gcggttttgg aaggtggacg cggcgaattt
      241 gaagccggag gactagagga acgcggcggt aattcccttg gtgagaaaaa aagcgatgac
      301 gccggccacc agatcgtcgg ccaccacgcc ccaacctccc ggcaggcggg agaccgcccg
      361 gccccagggc cccttgaaga cgtcgaagac ccgaaagaaa aaggcgccca ggagaaaggg
      421 cgcgggaagc cggggcaaaa gcagacaggc cgtccacacc ccggccactt catcgatgac
      481 gatgcgcggg tcgtccaggg aaagccattt ttcggcccag tggctcgtga cgatcgaaac
      541 gagaaggacg atcccgaaga gcgcccagag gagcggaccc cgggtcgggg ccgcccacag
      601 aagcgccgcc ccggccaggg tgccgagcag gccgccgcct ttccattttt tatggggcac
      661 cagatggtag ggcgcccagc ccagaaatcc cacggtggcc acggacagga cggcccattg
      721 ggtccagggg cgtccgggcg attcggtcac gggtattcct tcagcagggc ccagtgtttg
      781 cgcaaatagc tgacgccggt gaccagggaa aagagcgtgg cgccgaaggc catccagaac
      841 ggcacccagt cgagaacccg ggccgcgtcc ccgcgccatc cgccgagggc gtagagattt
      901 tcaatggaat ggcccccgaa gcgctccaac cccgaggcga ccacgagggc caccagaatg
      961 gcgatgatgg cggtgttttg aatcgaggtc ttgaatttcc ccccgtcgtc ggcggccatg
     1021 atttgcccct ggctggccgc cacggcccgc aggccggtga gcacgaactc ccgcccgacg
     1081 atggcgacga tcatccaggc cggaatgtga aggtcccgaa gttcgatgaa ggaaataaaa
     1141 gcggcggtga tgatcagttt gtcggccagg gggtccagga acacgcccag gggcgtgacc
     1201 atgtttcgcg tgcgggcgat gtggccgtcg accaaatcgg tgatgccggc ggcgataaag
     1261 atccccaggg cgccgaggcg cgcgtagacg ttgtcccagg agacgcacag caggaagaac
     1321 ggcaccgagg ccaggcgcgc cacggtgagt ttgttgggca gattcatcgg gacgctcccg
     1381 cccgggcgcg gggtttccgc cgggccggcg ccgagtcggc ggaggg
//
LOCUS       DAOQLS010000611         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_74641_length_1425_cov_3.951825, whole genome shotgun sequence.
ACCESSION   DAOQLS010000611 DAOQLS010000000
VERSION     DAOQLS010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74641_length_1425_cov_3.951825"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1425)
                     /locus_tag="PK362_09975"
     CDS             complement(<1..>1425)
                     /locus_tag="PK362_09975"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75195.1"
                     /translation="RPGLISDVDLHLLLPDDLASPAPDNPLGAAYEALYYGLEKVLGA
                     QDPKPLKDFQHAGDVEHAMAFWEPKSNQMGSSYTGVDALRVYGLTARPVAGNADLFEE
                     GVRPAYRQLLSRYRREILRGVILEREAQFESADSSLLRPDFKAGLGGVREYDTITWMS
                     RFLFPRGAEGLVGLLTGWTVLAAQRRVSLPQLLRARQAFLRQARLRDRRVDFDQLSRE
                     EKIQYVQDAGLLFDTLVSLLQSYWLEVRRPQDPEYLPFFLASAGWRPRSAANVQRQLD
                     EDLSRREGRLVAVRARRSGAEGMAEFAIEALRDRPGVLAVLSGLMAAHGFSIRAAEAQ
                     FERGRVFDRFYVHLGPLAAADKKAQDREIDRLGRAVENAAADVLAGERIELGRVAADF
                     ADAGRAYLFVLPRRHPVAATLAFFTGRAGARALTVLTSDRVLGGDEDQKEFLGTLHVI
                     TRWLAERGYNIVGDPSIRTQRGIVL"
BASE COUNT          268 a          470 c          469 g          218 t
ORIGIN      
        1 aggacgatgc cccgctgggt gcggatgctg ggatcgccca cgatgttgta gccgcgttcc
       61 gccaaccacc gggtgatcac gtggagcgtt cccagaaatt ccttttggtc ctcgtccccg
      121 cccaacacgc ggtcggaggt caggaccgtg agggcccggg cgcccgcccg gccggtgaag
      181 aaggccaggg tggccgccac cgggtgccgt cgcggcagaa cgaaaagata ggcgcggccg
      241 gcgtcggcga aatcggcggc cacgcggccc aattcaatgc gttcccccgc caaaacgtcc
      301 gcggccgcgt tctccaccgc tcggcccagg cggtcgattt cccggtcctg agcttttttg
      361 tccgccgcgg ccaggggacc caggtggacg taaaaacgat caaacacccg gccccgctca
      421 aattgggctt ccgccgcgcg gatgctgaac ccgtgggccg ccatcaagcc cgaaagaacc
      481 gccaagaccc cgggtcggtc ccgaagcgcc tcgatggcga attccgccat gccttcggcg
      541 ccggaacgcc gggcccggac ggccaccagg cgtccctccc gccgggacaa gtcctcgtcc
      601 aactggcgtt gaacgttggc cgccgatcgc gggcgccaac ccgcgctggc cagaaagaag
      661 ggcagatatt cgggatcctg gggccggcga acttcgagcc agtaggattg gaggagggac
      721 acgagggtgt caaacagcag tcccgcgtcc tggacgtact ggattttttc ttcccgggaa
      781 agttggtcga aatcgacccg gcggtcccga aggcgcgcct gacgaaggaa cgcctgccgg
      841 gcccgcaaca gttggggcag ggaaacccgc cgctgggcgg ccaggacggt ccaacccgtc
      901 aagaggccga ccaacccttc ggccccccgg gggaacagga agcggctcat ccaggtgatg
      961 gtgtcgtatt cccgcacgcc gccaaggccg gccttaaaat ccggacggag gagactcgag
     1021 tcagcgcttt caaattgggc ttcccgctcc aaaataaccc cccgcaaaat ctcgcgccgg
     1081 tagcgggaca ggagttggcg gtaggccggt cggaccccct cttcaaacag gtcggcgttc
     1141 ccggccacgg gacgggcggt caacccatac acccggagag cgtcaacgcc ggtgtaggaa
     1201 ctgcccattt ggttcgactt cggttcccag aaggccatgg cgtgttccac gtcgccagcg
     1261 tgttggaaat ccttcagggg tttggggtcc tgggcgccga gtactttttc cagtccgtaa
     1321 tacaacgcct cgtaggccgc gcccagggga ttgtccggcg cgggggaggc caggtcgtcc
     1381 ggaagaagaa gatgcaaatc gacatcggag atcaagcccg gccgg
//
LOCUS       DAOQLS010000612         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_74745_length_1424_cov_3.748722, whole genome shotgun sequence.
ACCESSION   DAOQLS010000612 DAOQLS010000000
VERSION     DAOQLS010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74745_length_1424_cov_3.748722"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PK362_09980"
     CDS             complement(<1..668)
                     /locus_tag="PK362_09980"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNC75196.1"
                     /translation="MPDIKPFRAIVYGAAHRKNIRSLVCPPYDVISPEGREALVARDP
                     LNFVRVELPAGDAETRYETARALWGRWLDHGVLRRESSPVFYVYESRFRSPVDGRPLK
                     RRGVFAALRVVPWGQGVYPHEKTLPTHKADRLSLFKALEAQTSPIQLLVRDSTSEIDR
                     LIAAQTKAAPWVQFTDEAKVTHRLWKWSGDASARRLAELFKTSPCAIADGHHRYETAL
                     IYSA"
     gene            complement(686..>1424)
                     /locus_tag="PK362_09985"
     CDS             complement(686..>1424)
                     /locus_tag="PK362_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNC75197.1"
                     /translation="RGLPTVSAVLVLNDPETGLPLAIMAAGRLTDIRTGAAGGVAAKH
                     LARQNSETLGLVGCGRQAVTQWRALAGRFPFKEIRVAGQTPEEARSFIAKNGTADGPA
                     WRATSVQEAAGADIVVTTTPGRAVVVSSPWVRPGAHVNAIGADAPGKQELDVALLKRA
                     RVFVDSPEQAFHSGEVNVGLSSGAMTPDDIAGGLGDVVAGRTPGRRSDADITVFDSTG
                     LAVQDVAVAAFVYRRARERSRGQTLTL"
BASE COUNT          244 a          460 c          498 g          222 t
ORIGIN      
        1 caggcgctgt agatcaaggc cgtttcgtag cggtggtgcc cgtcggcgat ggcgcaaggc
       61 gaggtcttga acaattccgc gagtcggcgg gccgacgcgt cgcccgacca tttccacaac
      121 cggtgggtca ctttcgcctc atcggtaaat tgcacccagg gcgcggcttt ggtttgggcc
      181 gcgatcaaac ggtcgatctc gctggttgaa tcccgaacca gaagctggat ggggctcgtc
      241 tgggcctcga gggccttgaa aagggacagg cggtccgcct tgtgggtggg aagggttttc
      301 tcgtggggat aaacgccctg gccccagggg accacccgaa gggcggcaaa aacgccccgc
      361 cgttttaaag ggcgcccgtc caccggagac cgaaagcggg attcgtagac gtaaaagacc
      421 ggggaggatt cccgcctcag aaccccgtga tcgagccatc ggccccagag ggcccgggcc
      481 gtctcgtagc gggtctcggc gtcgccggcc ggcagctcca cccggacgaa attcaacgga
      541 tcccgggcga ccagggcctc ccggccctcg ggggaaatga cgtcgtaggg cggacagacc
      601 agagaccgga tgtttttgcg atgggccgcg ccgtagacga tggcgcggaa gggtttgatg
      661 tcgggcatgg gagaaagaga cctttctaga gtgtcagggt ttgtccccgg gagcgttccc
      721 gggcccgtcg gtagacgaag gcggcgaccg ccacgtcctg caccgcgagc ccggtggaat
      781 caaaaacggt gatgtcggcg tcggaccgcc ggccgggcgt ccgaccggcc acgacgtcgc
      841 ccaacccccc ggcgatgtcg tcgggggtca tggcgccgga actcaatccg acgttgactt
      901 cgccggaatg aaaggcctgc tcgggggaat cgacaaacac ccgggcgcgc ttcaacagag
      961 cgacgtccag ttcctgtttg ccgggcgcgt cggccccgat ggcgttgacg tgggcgccgg
     1021 gacgcaccca cggggaggaa accaccaccg ctcggccggg ggtggtggtg acgacgatgt
     1081 cggccccggc cgcctcctgg accgaagtcg cccgccaagc cgggccgtcg gcggtcccgt
     1141 ttttcgcgat gaacgaacgg gcctcctccg gggtttgccc ggccacgcgg atttccttga
     1201 aaggaaaacg tccggcaagg gcccgccatt gggtgacggc ctggcggccg catcccacca
     1261 gtcccagggt ttccgaattt tgacgggcca aatgcttggc cgccacgccc ccggccgcgc
     1321 cggttcgaat gtccgtcagc cgtccggcgg ccatgatcgc caggggaagc cccgtctcgg
     1381 ggtcgttcag caccagcacc gccgacaccg tggggagccc ccgg
//
LOCUS       DAOQLS010000613         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_75160_length_1420_cov_4.169963, whole genome shotgun sequence.
ACCESSION   DAOQLS010000613 DAOQLS010000000
VERSION     DAOQLS010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75160_length_1420_cov_4.169963"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1420
                     /locus_tag="PK362_09990"
     CDS             <1..>1420
                     /locus_tag="PK362_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75198.1"
                     /translation="RRVVVHILDLHDVEEAQRRVAGLLDQLAASRPGPLSVVLEGAAG
                     SLDLSALRAFADDRVRGRVADLLLRKNVLSGAEAFALSTDRDLRLRGAEDPDLYRENV
                     AALRAALPHREADAARLAAAAGAFERLREKKLPPALLELERKTDAYEAGLLPLADYLG
                     ALVPSTGGGAEGPTLRRLQRVLDMERRIRPERVAEERRRLVERLAPLLSPSETRELTS
                     AGLDHRLGRGARDRFHAVLTGLAATHGISWSDYPAFAAYEASRATAEKIDPARLQAEL
                     AALAASRRTALATDDGARALLRSGEDLRLLRRLLAFGLLPEDLPTLRARQKDLEAWAE
                     SAVGTARDGGPSTDAPSLAAAVQRAALFYFLAERRNGALVDRLSETFEDASPAAAPAV
                     VVLVAGGFHAPGVRDALLGGGIGYIGLAPRCSGPATSASLEAFRPSDSPVDRLWSGAR
                     RALHRPLISAAEPLDDAPGAVDR"
BASE COUNT          175 a          493 c          499 g          253 t
ORIGIN      
        1 cccgtcgggt cgtcgttcac attctggatt tgcacgacgt ggaagaggcc caacgccggg
       61 tggcgggcct tctggatcaa ttggccgcct cccgccccgg accgctgtcg gtcgtgttgg
      121 agggcgcggc cggttccctg gacctgtccg cccttcgggc tttcgccgac gatcgggtcc
      181 ggggccgcgt cgccgatctt ttgcttcgta aaaacgtgtt gtccggcgcg gaagccttcg
      241 ccctgtccac ggatcgggac ctgcgcctgc gcggcgccga agatccggat ttgtatcggg
      301 aaaacgtcgc ggccctgcgc gcggccctgc cgcatcggga ggccgacgcc gcccgattgg
      361 ccgcggcggc gggggccttt gaacgccttc gggaaaagaa actgcccccg gccctcctcg
      421 agctggaacg aaaaaccgac gcctacgaag cggggttgtt gcctttggcg gattacctgg
      481 gcgcgttggt cccgtcgacg ggcggaggag cggaaggtcc cacgctccgg cgccttcaac
      541 gggtgttgga catggaacgg cggattcggc cggagcgcgt cgccgaggag cgccggcgac
      601 tggtggagcg attggcgccc ctcttgtctc cgtcggaaac ccgggaatta acgagcgcgg
      661 gcctggacca tcgcctgggc cgcggcgccc gggaccgttt ccacgccgtg ttgaccggat
      721 tggccgcgac gcacggaatt tcctggtccg actatcccgc cttcgcggct tacgaggctt
      781 cccgggccac ggcggaaaaa atcgatcccg cccgccttca ggcggaactg gcggcgctgg
      841 cggcttcccg ccggaccgct ttggcgacgg acgacggcgc ccgcgccctc ctccggtccg
      901 gcgaggatct tcggctgctt cgtcgcctcc tggccttcgg actgttgccg gaggatttgc
      961 cgacgcttcg ggcccggcaa aaagatctgg aggcctgggc cgaaagcgcc gtcggaaccg
     1021 cgcgggacgg cggtccttcg acggacgctc cctccctggc cgcggcggtt cagcgcgcgg
     1081 ccctttttta ttttctggcc gaacgccgaa acggcgccct ggtggaccgg ttgtcggaga
     1141 cttttgaaga cgcgtccccg gcggccgccc cggcggtggt ggttcttgtg gccggcggat
     1201 tccacgcccc cggcgtgcgg gacgcccttc tcgggggcgg catcggctac atcggtttgg
     1261 cgccccgctg ctccggtccc gcgacgtcgg cgtccctcga ggccttccgt ccaagcgatt
     1321 cgccggtcga tcgactgtgg tcgggcgccc gtcgggccct ccaccgcccg ctgatttcgg
     1381 cggcggaacc gttggacgac gcccccggtg ccgtcgatcg
//
LOCUS       DAOQLS010000614         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_75205_length_1420_cov_2.459341, whole genome shotgun sequence.
ACCESSION   DAOQLS010000614 DAOQLS010000000
VERSION     DAOQLS010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75205_length_1420_cov_2.459341"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..>1420)
                     /locus_tag="PK362_09995"
     CDS             complement(21..>1420)
                     /locus_tag="PK362_09995"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNC75199.1"
                     /translation="GGYTAAAGLVDDLVLLRRSLEKHGGARTARGALLTLERQVRLFG
                     FHLARLDFRQHSARVRAAAEALAGGRPATAEWAGLIAAGAPRAVPRIAGDGLALREME
                     TLAELQKTFGTRAADHYILSMTRSADDLWAALFLARRAGLIRREGNRWRSTVDIVPLF
                     ETVEDLSRAPALMAALWRHPFYRQILASRGGAQEIMLGYSDSNKDAGYLAANFHLFRA
                     QESLGRAAEKAGVLLRFFHGKGGTIDRGGGPAHRAILAAPRSVPGGRLRITEQGEVIA
                     YKYGRPAVARRNFEQMASAVLTAELAPPGAKLSPARRSVYEGVLAGLENGLLDRLDED
                     LAVDAFFLGDRVDHRGERDVAARGPLRSVHHRSCSESPRTSRDWSSCSFRVAFAPFCF
                     FCLPGFTFGAGSYTRLARCTCASGSSTSPTSSSRRSALPSRSPRISPLKALRPGCASS
                     ACSMRTLARRPAKRR"
BASE COUNT          262 a          504 c          471 g          183 t
ORIGIN      
        1 cccctcggca aggccgagca ctaccgccgc ttcgccggcc tgcgcgccaa ggtgcgcatg
       61 gagcaggcgc tcgacgcgca ccccggtcgc aaggctttca agggagagat cctcggcgag
      121 cgcgacggca aggcgctcct ccgcgacgac gacgtcggcg aggtcgagct cccgctcgcg
      181 cacgtgcagc gcgcgagcct cgtgtacgag ccggctccga aggtgaagcc gggcaagcag
      241 aagaagcaga agggcgcgaa cgcgacccga aacgagcatg aagaccagtc ccgcgaggtg
      301 cggggcgact cggagcagga acgatgatga acacttcgca aggggccgcg ggcggcgaca
      361 tcacgctctc cacgatgatc gacgcggtcg ccaaggaaaa aggcatcgac cgcaaggtcc
      421 tcgtcgagac ggtcgagcag gccattctca aggccggcca gaacgccttc gtaaacggac
      481 cgccgggcgg gcgaaagctt ggcccccggg ggcgccagct cggcggtcaa gaccgcgctc
      541 gccatctgct cgaaattgcg ccgggccacc gccgggcggc cgtatttgta ggcgatcacc
      601 tccccctgct cggtgatgcg aaggcgcccc cccggcacgg accgcggcgc ggccaaaatg
      661 gcccggtgcg cgggcccgcc cccgcggtcg atggtcccgc ctttgccgtg aaagaaccgg
      721 agaagaaccc cggctttttc cgccgcccgt cccagggact cctgggcccg gaacaggtgg
      781 aaattggccg ccaaatagcc cgcgtccttg tttgaatcgg aatagcccag catgatttcc
      841 tgggccccgc cgcgggacgc caaaatttgc cggtagaacg ggtgccgcca caacgccgcc
      901 atcagcgccg gcgcccggga caaatcctcg acggtctcga acagaggcac aatgtccacc
      961 gttgaccgcc accggtttcc ctcccggcgg atcaaccccg cccgacgagc caggaagagg
     1021 gccgcccaga ggtcgtcggc ggaccgcgtc atggacaaaa tgtaatggtc ggccgcccgg
     1081 gttccgaagg tcttttgcaa ttcggccagg gtttccattt cccgaagagc cagcccgtcc
     1141 ccggcgatcc gcggcaccgc ccggggggcg ccggcggcga ttaatccggc ccattccgcc
     1201 gtagcgggcc ggccgccggc caaagcttcg gccgcggccc ggacccgggc gctgtgttgt
     1261 cgaaaatcca gccgggccag atggaacccg aacaaacgga cctggcgctc cagcgtcagc
     1321 aaggcgccgc gggccgttcg ggcgccgccg tgtttttcca agcttcggcg aagaagaacc
     1381 aaatcgtcca ccaggccggc ggccgccgtg tagcccccct
//
LOCUS       DAOQLS010000615         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_75286_length_1419_cov_2.769062, whole genome shotgun sequence.
ACCESSION   DAOQLS010000615 DAOQLS010000000
VERSION     DAOQLS010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75286_length_1419_cov_2.769062"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PK362_10000"
     CDS             complement(<1..525)
                     /locus_tag="PK362_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC75200.1"
                     /translation="MMDNDVLLDPRREVAGFEPYVPGRDMENVKRLYKLKRVIKLASN
                     ENPIGPSPRAVAALRKSLPALHRYPDGFSTALRHALADHVGVKISQVSVGAGSDELIE
                     NLAKAYVSPADEIVVSEHAFLRYKMAGDLMGATVVTVPMNVMTHDLEAMAAAVTPRTK
                     FVFIANPNNPTGTYN"
     gene            complement(530..>1419)
                     /gene="pheA"
                     /locus_tag="PK362_10005"
     CDS             complement(530..>1419)
                     /gene="pheA"
                     /locus_tag="PK362_10005"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNC75201.1"
                     /translation="PGPLPREALDDIFSTVIANFRLLQKKVTVSYFGPEATYTHQAAI
                     KHFGPAEFVAEKSIGDVFDDVESGRADYGVVPIENSTEGVVNHTLDMFMESDLVICAE
                     RQDPIAHCLMAAPGTAKVKSIASHPQALAQCRKWLESHMAGVPVTAAASTSDAAAQAA
                     LNPGVAAIASPLAADIYHLKIIAPAIQDVKNNRTRFLIIGKKVSAPSGSGRDKTSILV
                     SLRDRVGALHDLLGLFKDAHLNLTKIESRPTKRRAWEYVFFIDFLGHILEPRVQKVFL
                     ELKRTTLMVKMLGCYPRAD"
BASE COUNT          279 a          414 c          427 g          299 t
ORIGIN      
        1 gttgtaggtt ccggtggggt tgttggggtt ggcgatgaaa acgaatttcg tccgaggggt
       61 gaccgccgcg gccatggcct ccaggtcgtg ggtcatgacg ttcatgggaa ccgtgaccac
      121 agtggcgccc atcaagtccc cagccatttt gtaacgcagg aacgcgtgct cgctgaccac
      181 gatctcgtcg gcggggctca cataggcctt ggccaaattt tcgatcagtt cgtccgaccc
      241 ggctcccacg ctgacctggc tgatcttcac ccccacatgg tccgccaggg cgtggcgaag
      301 ggcggtgctg aacccgtcgg gataacggtg aagggcggga agcgatttcc gtaaagcggc
      361 cacggcccgg ggagacggcc cgatcggatt ttcgttggag gccagtttta tcacccgttt
      421 cagtttgtag agccgtttca cgttttccat atcgcggccc ggcacataag gttcgaatcc
      481 ggccacttcc cgccgggggt ccagcaagac gtcgttgtcc atcattaaat taatccgccc
      541 ttgggtaaca acccaacatt ttaaccatca gcgtggtccg cttcagttca agaaaaactt
      601 tttgcacacg gggctccagg atgtggccca gaaagtcgat gaagaacaca tactcccacg
      661 cccgtcgttt cgtcggacgc gactcgattt tggtgaggtt caaatgggcg tccttaaaca
      721 ggcccaagag atcatgaagc gctcccaccc gatcccgcag agacaccaaa atggacgtct
      781 tgtcccggcc cgatccggac ggggcggaca cttttttccc gatgatcaaa aaccgcgtgc
      841 gattgttttt gacatcctga atggccggcg cgatgatctt gagatggtaa atgtccgcgg
      901 ccagagggct ggcgatggcg gccacgccgg gatttaaagc cgcctgggct gccgcgtccg
      961 aggtggacgc cgccgcagtc accggcacgc ccgccatgtg gctttcgagc catttccggc
     1021 attgggccag cgcctgggga tgggaggcga tgctcttgac tttcgccgtg cccggcgcgg
     1081 ccatcaaaca atgggcgatg gggtcctgcc gttccgcgca aatcaccaaa tccgattcca
     1141 tgaacatgtc gagggtgtgg ttcaccacgc cctcggtgga attctcaata ggcaccacgc
     1201 cgtaatcggc gcggccgctt tccacgtcgt cgaaaacgtc gccgatggat ttttccgcca
     1261 cgaactccgc ggggccgaaa tgcttaatcg ccgcctgatg ggtgtaggtg gcctccgggc
     1321 cgaaatacga gacagtgact tttttttgca acagccggaa attggcgatg acggtgctga
     1381 agatatcgtc caaggcttcc cgtggaagcg gcccggggt
//
LOCUS       DAOQLS010000616         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_75345_length_1418_cov_5.730007, whole genome shotgun sequence.
ACCESSION   DAOQLS010000616 DAOQLS010000000
VERSION     DAOQLS010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75345_length_1418_cov_5.730007"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PK362_10010"
     CDS             <1..870
                     /locus_tag="PK362_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNC75202.1"
                     /translation="RMARHRGMILAEIEKAGLPRELLYLPIVESEYQTFAVSRAGAVG
                     MWQFMTSTAKYAGLKINYWVDERRDPSKATRAALQTLKSLHDWFDDWHLALAAYNRGM
                     YGIQRDLEFTRSTDFALLSKRQGLPMETEQYVPKLMAAVLVGENAKAYGFETSATAAS
                     APPDEVVLEKPLDLKIAAACAKTTEEVLRELNPSIRLWCTPRNESRFSFNVPAGTRAR
                     FLEELNKVKDWTPSPGYLKYTVRKGDVLGRIAQRYRTTAAAIQRENKIQNAHRLRPGQ
                     VILIRPGRGFKDD"
     gene            892..>1418
                     /locus_tag="PK362_10015"
     CDS             892..>1418
                     /locus_tag="PK362_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNC75203.1"
                     /translation="MTPIKTEPILFGTDGWRGLIARDFTFANVRRVADALARALPSKS
                     RVVIGHDHRFLSEEFARTAAGVLAARGHRVLLGQGPTTSPALSFALKGAGARAGVMIT
                     ASHNPPAYNGFKIKLPPGRSADPLFTTKVETLVEPETPRPPSVELEQRDLAKPYLAHL
                     LSRLDRAFWKKARSTR"
BASE COUNT          275 a          467 c          432 g          244 t
ORIGIN      
        1 cggatggccc ggcaccgggg catgatcctg gccgaaattg aaaaggccgg gctcccccgg
       61 gagctgctgt atttgcccat cgtcgaaagc gaatatcaaa cgttcgccgt gtcccgggcc
      121 ggggcggtgg ggatgtggca attcatgacg tccacggcca aatacgcggg gctcaaaatc
      181 aactactggg tggacgaacg gcgcgatccc tcgaaagcca cccgcgcggc gcttcagacc
      241 ctgaaaagcc ttcacgactg gttcgacgat tggcatctgg ccttggcggc ctacaaccgg
      301 ggcatgtacg ggattcagcg ggatttggaa ttcacccgat cgacggattt cgcgctgttg
      361 tccaaacgcc aggggcttcc gatggaaacg gaacaatacg tgcccaagct gatggcggcc
      421 gtcttggtcg gggaaaacgc caaggcctac gggtttgaaa cgtcggccac ggccgcgtcg
      481 gccccgcccg acgaggtggt gctcgaaaaa cccttggacc tcaaaatcgc cgccgcctgc
      541 gccaaaacga cggaggaggt ccttcgggag ttgaacccct ccatccgcct gtggtgcacg
      601 ccccggaacg aatcccgttt ctctttcaac gtcccggccg ggacgcgcgc gcgctttctc
      661 gaggaactga acaaagtgaa agattggacg ccctcgcccg gctatctcaa gtacaccgtg
      721 cgaaagggcg acgtgctcgg ccgcatcgcc cagcgttacc gaacaaccgc ggcggccatc
      781 cagcgggaaa acaaaattca aaacgcccat cgacttcgac ccgggcaggt gattctgatc
      841 cgtccgggcc ggggattcaa ggacgactga cttccaccaa ggaccccccc catgaccccc
      901 atcaaaacgg aaccgattct ctttggcacc gacggctggc gcggcttgat cgcccgggat
      961 ttcaccttcg cgaacgtgcg gcgggtggcc gacgccctgg cccgcgcgtt gccgtccaaa
     1021 tcccgggtgg tgatcggtca cgatcatcgt ttcctgtcgg aagaattcgc tcggacggcc
     1081 gccggcgtcc tggcggcccg gggtcaccgg gttcttctcg gccagggtcc gaccacgtcg
     1141 ccggccctgt ccttcgcgtt gaagggagcc ggcgcccggg cgggcgtcat gatcaccgcg
     1201 agccacaacc ccccggccta caacggtttc aagattaaac tgccccccgg tcggtcggcc
     1261 gatccgctct ttacgaccaa ggtggaaacc ttggtggagc ccgaaacgcc tcggccgccg
     1321 tcggtggaat tggaacaacg ggatttggcg aagccctatc tggcccattt gctctcccgg
     1381 ctggaccggg ccttctggaa aaaagcgcga tccacccg
//
LOCUS       DAOQLS010000617         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_75466_length_1417_cov_4.527166, whole genome shotgun sequence.
ACCESSION   DAOQLS010000617 DAOQLS010000000
VERSION     DAOQLS010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75466_length_1417_cov_4.527166"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PK362_10020"
     CDS             complement(<1..1373)
                     /locus_tag="PK362_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75204.1"
                     /translation="MATVAAVYDRMGLSTAYYHNMAHNLGVTHTLLQMLRGRPATSRD
                     DVKAAFLAGLLHDFHLRDMETAKNLPGGLEAEVTVTLRQLSALWGVERSVPKSEEALQ
                     RFHDEAYFNKIRAEFGDALRGEFTAFLGNQTPAAVLPVVEALIRRTDFPRKNADGTDE
                     KSPQTINAWFEAALNRLPAGVDHDTVRDLAGYLQTADLSVTNVQLTFDANVAVIDTGL
                     AVEKPGQASEKGTFIFLINILKPHLGRLANLPLSARRHLFDVFERFANVPGAFDVPAV
                     MGAEEKESATARWNREKEEVANILKVTESPLRRLARILSSPIPGLGRFGQVPLVSGNY
                     ETAINENPAKTLLWSAPFGENLRLLLLAYPLMMVAGAPAWALASTALMALLLTRDYHQ
                     KVFYETEDGIVRQRTATWMERLGIFSWFTAFGVGSLAVTVLMTGAFVPSFAGLGPAEI
                     LQNLPVLA"
BASE COUNT          265 a          403 c          481 g          268 t
ORIGIN      
        1 gccaggacgg ggaggttttg aaggatttcc gccggaccaa gaccggcgaa agacggaacg
       61 aaggcgcccg tcatgaggac cgtcaccgcc aaggacccaa ccccgaaggc ggtgaaccag
      121 gagaaaatgc ccagacgctc catccacgtg gcggttcgtt ggcgaacgat cccgtcctcg
      181 gtctcgtaga aaactttttg atggtaatcg cgcgtcaaca acagggccat gagcgcggtg
      241 ctggccaggg cccaggccgg ggctcccgcc accatcatca gcggataagc cagcaggagc
      301 aagcgcagat tttccccgaa gggggcggac caaagaagcg ttttggcggg attttcgttg
      361 atggcggttt cgtagttgcc ggagacgagg gggacttggc cgaaacggcc caggccggga
      421 atcggggagg aaaggatccg ggccagtcgc cgcagggggc tttcggtgac tttgagaatg
      481 ttcgccactt cctctttttc gcggttccag cgcgccgtcg ccgattcctt ttcttcggcg
      541 cccatcaccg cggggacgtc gaaggcgccg ggcacgttgg cgaatcgttc aaaaacgtcg
      601 aagagatgtc gacgggccga cagggggagg ttggccaacc ggcccaggtg gggtttcagg
      661 atgttgatca ggaaaatgaa ggtccctttt tcgctggcct gccccggctt ttccaccgcc
      721 agccccgtgt cgatcacggc gacgttggcg tcgaaggtca attggacgtt ggtgacgctt
      781 aaatcggcgg tttgaaggta accggccagg tcccggaccg tgtcatggtc gacgccggcg
      841 ggaaggcggt tcagggcggc ttcgaaccag gcgttgatgg tttgggggct cttttcgtcg
      901 gtgccgtcgg cgttcttgcg ggggaaatcc gtgcggcgaa tcagcgcttc cacgaccgga
      961 agaacggcgg ccggggtttg gttcccgagg aaagccgtga attctccgcg aagggcgtcg
     1021 ccgaattccg cccggatttt attgaaataa gcctcgtcgt gaaaacgttg aagcgcctct
     1081 tcgctcttgg gcacgctccg ttccaccccc cacagggcgc tcaattgccg gagggtgacg
     1141 gtgacttcgg cttccagtcc gcccggaaga ttcttggcgg tttccatgtc ccgcaggtgg
     1201 aaatcgtgaa gaaggccggc caggaaagcg gctttcacgt cgtcccggga ggtggccggg
     1261 cgcccgcgca acatttgaag aagggtgtgg gtgaccccca aattgtgggc catgttgtgg
     1321 tagtacgccg tggagagccc catccgatca tagaccgccg ccacggttgc catcagtcgt
     1381 cgagcggcgt cgacttcggc cgccgacagg ccgagcc
//
LOCUS       DAOQLS010000618         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_75577_length_1416_cov_4.208670, whole genome shotgun sequence.
ACCESSION   DAOQLS010000618 DAOQLS010000000
VERSION     DAOQLS010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75577_length_1416_cov_4.208670"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PK362_10025"
     CDS             <1..1405
                     /locus_tag="PK362_10025"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC75205.1"
                     /translation="VRCLQELYAGNDAGAQQALEILNAKDSWAQSRANYLSGLVSLQS
                     GFVESRSDHFVVRVPAEDAFLGDYALPALENAHAKFTDVLGAFSSGTAVVEIYPTVES
                     FSFASTLSPEAIARTGTVAVFKFGRLMILSPRATARGYRWLDALVHQYARAAVRRLSG
                     SRCPVWLEEGTARYLTIVWRRTDEFVLPPYDEGLLAAAASTAPAPGSVPWVPFDKMEP
                     AFVYLPDPATVALAESEASDAVAYVVDEHGLDGLRSLLTALKDRPREEAFAAAVGSTE
                     EELEAGWRESLAEETLNAPAGALSSAVQLKPLEDAVWAGAAAEPFLRAGDRLRQQNQW
                     AAAAAQYRKGLEAAPDNGLLMTRLARTLLASGTPEAAEPLLRRAAEKNPAFAEPFVLM
                     GDTFFDDGRYEEAQAVLQSALEINPFDPKVHEILGLIAVDVGNFAEARRSLQLALRFD
                     PENDAVAQALKRMPKRR"
BASE COUNT          220 a          475 c          484 g          237 t
ORIGIN      
        1 cgtgcgatgt cttcaggagc tctacgcggg caacgacgcg ggcgcccagc aggccctgga
       61 aatattgaac gccaaagaca gctgggccca gtcccgggcc aattatctgt cgggtctggt
      121 gtcccttcaa agcgggttcg tcgaatcccg gtcggatcat ttcgtggtcc gggtgccggc
      181 ggaggacgcg ttcctcgggg attacgcttt gccggccctg gaaaacgccc acgcgaaatt
      241 cacggacgtc ctgggcgcgt tctcttcggg cacggcggtg gtggagatct accccaccgt
      301 ggaaagtttt tccttcgcct cgacgctttc cccggaggcc atcgcccgga cgggcaccgt
      361 ggccgtgttt aaattcggtc ggctcatgat tctctcgccc cgggccacgg cccggggcta
      421 ccgctggctg gacgccctgg tccatcaata cgcccgggcg gcggtccgcc ggttgtcggg
      481 atcccgctgc ccggtgtggc tcgaggaagg cacggcccgg tacctgacga tcgtctggcg
      541 gcggacggac gaattcgttc tgccccccta cgacgaaggg ttgctggcgg cggccgcttc
      601 gacggcgccc gcgcccggtt cggtcccctg ggtccccttc gataaaatgg agccggcctt
      661 cgtctacctg cccgatccgg cgacggtggc cctggccgaa tccgaagcct ccgacgcggt
      721 ggcctacgtg gtggacgaac acgggctgga cggcctgcgg tccctgctca cggcgctgaa
      781 agaccgtccg cgggaggagg ccttcgccgc ggccgtcgga tcgacggaag aagagctgga
      841 ggcgggttgg cgggaatcct tggccgagga aaccctgaac gcgccggcgg gggccttgtc
      901 gagcgcggtt caactgaagc cgctggagga cgcggtgtgg gccggcgccg ccgcggagcc
      961 gttccttcgg gccggcgacc gcctgcgcca acaaaatcaa tgggccgccg cggccgccca
     1021 ataccggaag ggattggagg cggcgcccga caacgggttg ttgatgaccc gcctggcccg
     1081 gacgctcctg gcgagcggga cccccgaggc cgccgaaccc ctcctgcgcc gcgccgccga
     1141 aaagaacccg gccttcgcgg aacccttcgt gctgatgggc gacacgtttt tcgacgacgg
     1201 ccgctacgag gaagcccagg cggttttgca gagcgccctg gaaatcaacc cctttgatcc
     1261 gaaggttcac gaaatcctgg ggctcatcgc cgtcgatgtc ggaaatttcg cggaggcccg
     1321 acggtctctt cagttggcgc tccgattcga tccggagaac gacgccgtgg cccaggcgtt
     1381 gaaacggatg cccaaacgcc ggtgacccgc cccccg
//
LOCUS       DAOQLS010000619         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_75917_length_1413_cov_3.502946, whole genome shotgun sequence.
ACCESSION   DAOQLS010000619 DAOQLS010000000
VERSION     DAOQLS010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75917_length_1413_cov_3.502946"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK362_10030"
     CDS             <1..255
                     /locus_tag="PK362_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNC75206.1"
                     /translation="NVEFVQITDPGHARVRVWERGAGATLACGTGACGVAVAGVLTGR
                     LHRKVRLSLPGGELGIEWAADNHVFLSGPAAFVYDGVLAV"
     gene            265..1167
                     /gene="dapA"
                     /locus_tag="PK362_10035"
     CDS             265..1167
                     /gene="dapA"
                     /locus_tag="PK362_10035"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNC75207.1"
                     /translation="MFEGSCVALVTPFKDGLVDDKKLAELVEFQIAGGTAAIVPCGTT
                     GESATLSHKEHNEVVEKVISFARKRVKVIAGTGSNSTREAIELTRHAQAAGADGALLI
                     TPYYNRPTQRGLVEHFRAIARAVDLPLVLYNVPSRTGVNMLPPAIIELARSEKNIVGV
                     KEASGSVDQTTEIIEALPETFTVLSGDDSLTLPLMAVGARGVISVLANLIPKDVAALC
                     AAWKKGDAAEARRLHLKMYPLARAMFTETNPIPVKTAMGWLELCSAEMRLPLVAMEKA
                     NAEKVETALRAYGLIGPRRKHAGC"
BASE COUNT          275 a          424 c          441 g          273 t
ORIGIN      
        1 aacgtggaat tcgtgcagat cacggacccc ggccacgccc gcgtgcgggt atgggagcgg
       61 ggcgcgggcg cgaccctcgc ctgcggcacg ggcgcctgcg gggtggccgt ggcgggcgtc
      121 ctcacgggcc gcctgcaccg gaaagtccgc ctctccctcc ccggtggcga attgggcatc
      181 gaatgggcgg ccgacaacca cgtctttttg tcgggccccg cggcttttgt ttacgacggc
      241 gtcttggccg tctaaggagc gtttatgttc gaaggatcct gcgttgcgtt ggtgaccccg
      301 ttcaaggacg gtctcgtcga cgacaagaag ttggccgaat tggtcgaatt tcaaatcgcc
      361 ggcggcaccg ccgccatcgt cccctgcggc acgacgggcg agtcggcgac cctttcccac
      421 aaggaacaca acgaagtcgt ggagaaagtc atctccttcg cgcggaagcg cgtgaaggtc
      481 atcgcgggta ccggttccaa ctccacccgg gaagccatcg aactcacccg gcacgcccag
      541 gcggccgggg cggacggcgc gctcctgatc acgccctatt acaaccggcc cacccaacgg
      601 gggctggtgg agcacttccg cgccatcgcg cgagcggtgg acctgccgct cgtgctttac
      661 aacgtgccga gccgcaccgg cgtcaacatg ctgccgcccg cgatcatcga attggcccgg
      721 tcggaaaaaa acatcgtggg ggtgaaagaa gcctcgggca gcgtggatca aaccacggaa
      781 atcatcgagg cgctccccga aacgttcacg gtgctgtcgg gcgacgactc cctgacgctc
      841 cccctgatgg ccgtgggggc ccggggcgtc atctcggtct tggccaacct gatccccaag
      901 gacgtggccg ccctctgcgc cgcttggaaa aagggcgacg cggcggaggc ccgccgcctg
      961 catcttaaaa tgtatccctt ggcccgggcc atgttcacgg aaacgaaccc gatccccgtc
     1021 aaaacggcga tgggttggct ggagctttgc tcggcggaga tgcgcctgcc gctcgtggcc
     1081 atggagaaag ccaacgccga aaaagtggaa acggcgcttc gggcctacgg gttgatcggg
     1141 ccccggcgga aacacgcggg atgttaataa ttcgtcgaat aactaaacat cagtatttta
     1201 ttttcgcctt ttctttggtt ttgtcttgcg ggtccttgaa tgcgaagacc atttacatat
     1261 attcgcgtgt cgagagtgtt caaattcaac aattgccgga tgtggaaagt agtgttttga
     1321 aaaccattga taaaggaacg gtccttgggg tttcgtgtat cggtaaatct gttcgaatac
     1381 aaaatacagt gggccaatgg tgcatggcca ctc
//
LOCUS       DAOQLS010000620         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_76018_length_1412_cov_3.957259, whole genome shotgun sequence.
ACCESSION   DAOQLS010000620 DAOQLS010000000
VERSION     DAOQLS010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76018_length_1412_cov_3.957259"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..>1412
                     /locus_tag="PK362_10040"
     CDS             81..>1412
                     /locus_tag="PK362_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75208.1"
                     /translation="MSTLVPGVRMSWSVLGPLAPTLAVSSSVSAGRLRWAAVLGPIFS
                     LGAAGAAGLAAAAHAFPPLGDLALWAFSAVNAVLALTSWSDFTAFWRGRAVALHCGVI
                     GVVFGGPNPGARAMPTALSSLLDKATRRTLHRGGQSGGVASVVARENGHIEIGFRVEK
                     TAKEKSRRSPLAELMARSRDRLGAAAERDGFGGLNKIVAIAHTRYGTNLAPPVAKNAH
                     PHESEARTESIFFVGDRNRHDAYERAWEKPQGPAEIKNVSAPRGVVIAHNGDDNATAA
                     IRWADGGVMEISNDDDAEISERMTGFRPSGRGDSPQIATRLDRWVTQGSVRGALALAL
                     LMTGLSALPRGAAKKFDSATRDRVPTARAIEGALQVHALESFGKDVAALLARFPEAKS
                     LEDLYGRAGGPSPLEIDSLELEASALPAPGSPLEALRDRLAHQCPAVLSGLA"
BASE COUNT          222 a          456 c          484 g          250 t
ORIGIN      
        1 gggcgggaca tgtggcggtt ttggaaggat gggcgccggg tcgttggatc cggcgtctgg
       61 cggagtacga tccccaagga atgtcgacat tggtgcccgg ggtcagaatg tcgtggtccg
      121 tcttgggtcc tttggcgccc accttggcgg tatcttcgag cgtttccgcg ggtcgtcttc
      181 gctgggcggc ggtcctgggt cccatattta gtctcggagc cgccggggcg gcgggcctgg
      241 ccgcggccgc ccacgcgttc ccgccattgg gcgatttggc cctttgggcc ttttccgcgg
      301 tcaacgcggt cctcgcgctc acgtcctggt cggattttac cgccttctgg cgggggcgcg
      361 ccgtcgccct gcactgcggg gtcatcgggg tggttttcgg cggccccaac ccgggcgccc
      421 gggcgatgcc gacggccttg tccagtctcc tggacaaggc cacgcgccga acccttcacc
      481 ggggggggca atcgggcggc gtggcctcgg tcgtcgcccg cgaaaacgga cacatcgaaa
      541 tcggttttcg cgttgagaaa acggccaagg aaaagagccg gcgttcgccg ttggccgaac
      601 tcatggcccg aagccgggat cgtttgggcg ccgccgccga acgcgatggg ttcggcgggt
      661 taaacaagat cgtggccatc gcccacacgc gttacgggac caatctggcg cctcccgtgg
      721 ccaagaacgc ccatccccat gaaagcgaag cgcgaacgga atccattttc ttcgtgggcg
      781 accgaaaccg ccacgacgcc tacgaacgcg cctgggagaa gccccaggga ccggccgaaa
      841 tcaaaaacgt ttcggcgccc cggggggtcg tcattgccca caacggggac gacaacgcca
      901 ccgcggccat ccgttgggcc gacggaggcg taatggaaat ttccaacgac gacgacgccg
      961 agatcagcga acggatgacc ggttttcgtc cctcgggccg gggcgactct ccgcaaatcg
     1021 ccacccgttt ggatcgctgg gtcacccagg gatccgttcg cggggcgctg gccctggctc
     1081 ttctgatgac gggtctgagc gcgttgcccc ggggggccgc aaaaaaattc gattcggcga
     1141 caagggaccg ggttccaacc gcgcgggcga tcgaaggggc cctgcaggtg cacgcgctgg
     1201 aatcctttgg gaaggacgtg gccgccttgc tcgcccggtt tccggaagcc aaatccctgg
     1261 aagaccttta cggccgggcc ggcggtccat ctccgctcga aatcgattcc ttggaattgg
     1321 aagcctccgc gctccccgcc cccggatcgc cgctggaagc cctgcgcgac cggttggccc
     1381 atcaatgccc ggcggtgttg agtgggctgg cg
//
LOCUS       DAOQLS010000621         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_76022_length_1412_cov_3.780398, whole genome shotgun sequence.
ACCESSION   DAOQLS010000621 DAOQLS010000000
VERSION     DAOQLS010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76022_length_1412_cov_3.780398"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PK362_10045"
     CDS             <1..615
                     /locus_tag="PK362_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC75209.1"
                     /translation="DNAGLEEVLHESGWEWLDVVPSHMDLYGAEVELVNAESRETRLS
                     AALSRFQRAYKYVIIDCPPALNLLTLNALTAADSVLIPMPCEYYALEGLSMLVKTIEK
                     IRAGLNPRLELEGVLVTMFDGRANLAQQVLAEIKRFFGTKVYQTVIPRNVRLAEAPSH
                     GQPAIVYDRQSTGASAYLTFAREFLERRGTPVPVPEAAEPVKAS"
     gene            633..>1412
                     /locus_tag="PK362_10050"
     CDS             633..>1412
                     /locus_tag="PK362_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNC75210.1"
                     /translation="MAHKALGRGLEALLKPVMGGGGGDDRTSISKIAVEKIRPNRHQP
                     RVRFTPDSLQELADSIKIHGLAQPLLVSPSAVPGEFELVAGERRLRAARMAGLVDVPC
                     VIRPVTDRERHELSLIENIQRENLNAIEEAESMRKLMDEAGLTQEDMAKRLGKSRSGI
                     ANKLRLLELSQMIRNALMEGLLTEGHARALLGLEGKQLQEDLAKRVVQEKLNVRDVER
                     LVAEWSAAPERPRPAKRKNPDVRVLEEELQRHLGRRVVVDSR"
BASE COUNT          275 a          451 c          458 g          228 t
ORIGIN      
        1 gacaacgcgg gcctggagga agtgcttcac gaaagcgggt gggaatggct ggacgtcgtg
       61 ccctcccaca tggaccttta cggcgccgaa gtggagctgg tcaacgccga aagccgggaa
      121 acgcggctgt cggccgcctt gtcccgtttt caacgggcct acaaatacgt catcatcgac
      181 tgcccgccgg ccttgaacct tttgactttg aacgccttga ccgcggccga ctcggtcctc
      241 atccccatgc cctgcgaata ttacgccctg gaagggctct ccatgctggt caaaaccatc
      301 gagaaaattc gggccggctt gaacccccgg ttggagctcg aaggcgtgct cgtgaccatg
      361 ttcgacggcc gggccaatct ggcccagcag gtcctcgcgg aaatcaaaag gtttttcgga
      421 accaaagtgt atcagacggt gattccccgc aacgtccgtc tggccgaggc cccgagccac
      481 ggccagccgg cgatcgtgta cgaccggcaa tccaccggcg cgtcggccta tttgaccttc
      541 gcccgggaat tcctggagcg gcgcggcacg cccgttcccg tgcccgaggc ggcggaaccc
      601 gtgaaggctt cgtaatcaaa gggagaatcg cgatggcgca caaagcgttg ggtcggggcc
      661 tggaagccct gttgaagccc gtgatgggcg ggggcggggg cgacgaccga acctccatca
      721 gcaagatcgc cgtggaaaag atccgcccga accgccacca gccgcgggtg cgctttacgc
      781 cggactccct gcaggaactg gcggactcca tcaagattca cggcctggcc cagccgttgt
      841 tggtctcccc gtcggccgtg cccggcgagt tcgaattggt ggccggcgag cgccggctgc
      901 gggccgcccg catggcgggc ctcgtggacg tgccctgcgt catccgcccc gtgaccgacc
      961 gggagcgcca cgagctctcc ctgatcgaaa acatccagcg ggaaaacctg aacgccatcg
     1021 aagaagcgga gtccatgcgc aaattgatgg acgaggcggg attgacccag gaggacatgg
     1081 cgaaacgcct cggcaaaagc cgttccggga tcgccaacaa gctgcgcctt ttggaattgt
     1141 cccaaatgat ccgaaacgcc cttatggaag gcctgttgac cgagggacac gcccgcgccc
     1201 tgttggggtt ggagggcaaa cagcttcagg aagacctggc caagcgcgtc gtccaggaaa
     1261 aattgaacgt gcgggacgtg gaacgcctgg tggccgagtg gtcggccgcc ccggagcgcc
     1321 cgcgcccggc caaacgcaag aaccccgatg tgcgcgtttt ggaggaggag ctccaacgcc
     1381 acctgggacg ccgggtcgtc gtggactccc gg
//
LOCUS       DAOQLS010000622         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_76034_length_1412_cov_3.379514, whole genome shotgun sequence.
ACCESSION   DAOQLS010000622 DAOQLS010000000
VERSION     DAOQLS010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76034_length_1412_cov_3.379514"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PK362_10055"
     CDS             complement(<1..393)
                     /locus_tag="PK362_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75211.1"
                     /translation="MHGRDSSRTLAGGTRINPAPRLVGLGTAFPPFRVSQGEALRFLL
                     KRFPVSPAARTLHARVFAHESVETRRLAVDRLAEVADRDLDRTHRRFEKAAVDLSGRS
                     LAAALRRGGRSPRQIDFLAAATCTGYLCP"
     gene            complement(345..1283)
                     /gene="trxB"
                     /locus_tag="PK362_10060"
     CDS             complement(345..1283)
                     /gene="trxB"
                     /locus_tag="PK362_10060"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNC75212.1"
                     /translation="METRPLIIIGSGPAGFTAALYASRANLKPLVLGGLFKGGQPGGQ
                     LMTTTDVENFPGFPDGVEGPELMKRMRQQAQRFGAEVLDKDVESVDFSVRPFKITTDE
                     ADYRARAVILSTGATAKRLHNPAEAKFWNRGISACATCDGALPIFRNKPIVVIGGGDT
                     AMEEALYLTRFGSEVVVLHRREEFRASRVMLERAKKNPKIKFKVPYALEDTHGEKTLE
                     QLKIKNLQTGAVETLAAAGLFLAIGHEPNTALFKGQIHLDDVGYVKTDGHARTNIEGV
                     FAAGDCVDHVYRQAITAAGQGCMAAIQAERWLAEQE"
BASE COUNT          247 a          411 c          492 g          262 t
ORIGIN      
        1 cggacagaga tagcccgtgc aggtggcggc ggccaaaaaa tcgatttgcc ggggggaccg
       61 tccgccgcgc cgcagggccg ccgccaggga ccgtcccgag aggtccaccg ccgctttttc
      121 aaatcggcga tgggttcggt ccaaatcccg atcggccacc tccgcgaggc ggtcgacggc
      181 caggcgacgg gtttcgacgg attcgtgggc gaagacgcgg gcgtgaaggg tccgggcggc
      241 gggggaaacg gggaaacgct tcagcaggaa ccgaagggcc tcgccctggg aaacgcgaaa
      301 gggcggaaag gcggtgccga gaccgacgag acggggcgcg ggatttattc ttgttccgcc
      361 agccagcgtt cggcttgaat cgcggccatg cacccctgcc ccgccgccgt gatggcctgg
      421 cggtagacgt ggtcgacgca atccccggcg gcgaaaacgc cctcgatgtt ggtgcgggcg
      481 tggccgtcgg ttttcacgta accgacgtcg tccaaatgga tttggccttt gaacagggcg
      541 gtgttcggtt cgtggccgat ggccaaaaag aggcccgcgg cggccagggt ttccaccgcg
      601 ccggtttgga ggttcttgat tttgagctgt tccagcgtct tttcgccgtg ggtgtcttcc
      661 aacgcgtagg gcactttgaa tttaattttg gggttcttct tggcccgttc cagcatcacc
      721 cgggaggccc ggaactcttc ccgccggtgg aggaccacga cttcggaccc gaagcgggtc
      781 aggtaaaggg cctcctccat ggcggtgtcc ccgccgccga tcacgacaat gggcttattg
      841 cggaaaatcg gcagggcccc gtcgcaggtg gcgcaggcgg aaatgccccg gttccagaac
      901 ttggcctcgg cggggttgtg caggcgtttg gcggtggcgc cggtggagag gatcacggcc
      961 cgggcgcggt agtcggcctc gtcggtcgtg attttaaaag gacggacgga aaaatccacc
     1021 gattccacgt ccttgtcgag gacttcggcc ccgaaccgtt gggcctgttg gcgcatccgc
     1081 ttcatcaatt ccggcccttc gacgccgtcg ggaaagccgg ggaaattttc cacgtcggtg
     1141 gtggtcatca actggccgcc cggctggccg cctttaaaca ggccgcccaa caccagaggc
     1201 ttcaaattgg cccgggacgc gtagagggcg gcggtgaaac cggcgggacc ggaaccgatg
     1261 attattaagg gacgcgtttc cattcgaggg acctcttctt attcaaacgc ccgcgaccgg
     1321 ggggagttcc cggcgcgggg atcaatattc ttttttgctg gccgacaggt aacggagaat
     1381 gtcgcggatg agcagaatgc tgacgggggt cc
//
LOCUS       DAOQLS010000623         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_76691_length_1406_cov_3.727609, whole genome shotgun sequence.
ACCESSION   DAOQLS010000623 DAOQLS010000000
VERSION     DAOQLS010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76691_length_1406_cov_3.727609"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..>1406
                     /locus_tag="PK362_10065"
     CDS             123..>1406
                     /locus_tag="PK362_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75213.1"
                     /translation="MPDGLPLVSSRDYVKDGQTYRDTVTTWNIKVLKFDDVGNLVQSL
                     VYRTQTTTVLASARPSDPPIEVTVTETEARAGTRMGGFGGARSGAVILRATDSDSVWA
                     DKMNNGDDRNINGHYLPWLNSFAVESLKRLAGLHRNRARAADDIARSTTDVDIAAKRM
                     ETDTADLTRADQAVSSARSALVAAQNAARSFRAEFESMDSTGRAVLEAAKARMTGRLQ
                     TDAAALAKTFSGLDLILNGPLRLTEEEALALVTRGAFDHAGQSLSLGALASAKVKTNL
                     NNEEHFAQLSVANPQKSVSLSWKGDLRLNGAPVTKTALSAVLTPNALAAPPADGSVSG
                     LSVGEGGRLTVNRTLAQTQDAAGRITSQNTETLTLSRTDGKTMTRRTRQETAGHRYDA
                     RGQLIGYVRTTREDGKDPVVETLTGATYDDTGRQTS"
BASE COUNT          300 a          498 c          399 g          209 t
ORIGIN      
        1 acgccgccaa caccgccaaa gaagacgctc tggcccgcct ggcgacggcc gaactccaaa
       61 aagcgcggat ggatgccgcg atatcgcaat tccgatccga tcacaaagaa atcgcgcgct
      121 ccttgccgga cggtctcccc ctggtttcca gccgggatta cgtcaaggac ggtcaaacct
      181 accgggacac cgtcaccacc tggaacatca aagtcctcaa attcgacgac gtcggcaacc
      241 tggtccagtc gctggtttat cgaacccaaa ccacgacggt tctcgcctcg gcccggccat
      301 cggatccccc gatcgaggtc accgtgacgg aaaccgaagc gcgcgccggg acccgaatgg
      361 gcggttttgg cggggctcgc agcggggccg tgatccttcg ggcgacggac agcgacagtg
      421 tgtgggcgga caaaatgaac aacggcgacg accgcaacat taacggccat tatctcccct
      481 ggttgaattc cttcgccgtc gaatcgttga aacgcctagc gggccttcac cggaaccgcg
      541 cccgggccgc cgacgacatc gcccggtcga ccaccgacgt cgatatcgcc gccaaacgaa
      601 tggagaccga cacggccgat ttaaccaggg ccgaccaagc cgtgtcatcg gcccgatcgg
      661 cccttgtcgc ggctcaaaac gccgcccgat cgttccgggc ggaattcgag tccatggact
      721 ccaccggccg ggcggttttg gaagccgcga aagcgcggat gaccggccga cttcaaaccg
      781 acgccgccgc cctggccaaa acgttttcgg gattggattt aattctcaac ggccctctcc
      841 gattaacgga agaagaagct ctggccttgg tcacccgcgg agccttcgac cacgcggggc
      901 aatccctttc cctgggggcg ttggcttccg ccaaagtcaa aacgaacctt aacaacgaag
      961 aacacttcgc ccaactttcg gtcgccaacc cccaaaaatc cgtgtccctt tcctggaagg
     1021 gcgatctgcg gttgaacggc gctcccgtga cgaaaaccgc cctgtccgcc gtcttgacgc
     1081 cgaacgcctt ggcggccccg cccgccgacg gttccgtgtc cgggctttcc gtcggggagg
     1141 gcggccgttt aaccgtcaac cgcaccctgg cccaaaccca ggacgccgcc gggcggatca
     1201 cgagccaaaa caccgaaacg ctcaccctct cccgcaccga cggcaaaacg atgacccgcc
     1261 gaacccggca agaaacggcc gggcaccgat acgacgcacg gggccagttg atcggctacg
     1321 tgcggaccac ccgggaggac ggcaaagacc cggtggtgga aaccctcacc ggcgcgacct
     1381 acgacgacac cggccgccag acctcc
//
LOCUS       DAOQLS010000624         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_76798_length_1405_cov_4.903704, whole genome shotgun sequence.
ACCESSION   DAOQLS010000624 DAOQLS010000000
VERSION     DAOQLS010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76798_length_1405_cov_4.903704"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..518)
                     /locus_tag="PK362_10070"
     CDS             complement(144..518)
                     /locus_tag="PK362_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC75214.1"
                     /translation="MSTTVNAKKELGLRIRSLRKRQGLTQEEMADKCGLHWTYIGGLE
                     RGERNPTLTTMQKVAEGLGVGLTELLNAKAFQEGPVVDQDTNEGKLMRLLRRENGDTV
                     ALAAGILREVLSWRDKNASHRN"
     gene            635..1396
                     /gene="rsmA"
                     /locus_tag="PK362_10075"
     CDS             635..1396
                     /gene="rsmA"
                     /locus_tag="PK362_10075"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNC75215.1"
                     /translation="MRTKWGQNFLAHPPTMRRIAEALELAPGDAVLEIGPGRGALTQC
                     LSEKAGRLVAVELDRDLAVRLPARFPKAEIVNADFLDWPPPEVPAGTFRVIGNLPYSA
                     AAPILQKVLAWTGWDRAVFMVQKEVADRIAAPAGTKAFGILSLAVQSRATTETLFDVP
                     PGAFNPPPRVTSTVLRFRRRPVPLFRDEAAFFNVVKAGFAQRRKTLANNLAGSFSKDR
                     EAVEKILTRCGVDPRARAEVVSLPQWVALAEAIGS"
BASE COUNT          234 a          410 c          451 g          310 t
ORIGIN      
        1 cccgtgggcc gggtttacat cggctgggcg tcccggcgaa aaacccgggt ctgggaaaaa
       61 aacttattcg gggaacgggc ggcgattcgc gagcgcgcgg ccctggaggc actcaacgcg
      121 ctgtgggaaa atttgggggc ggattagttg cggtgggagg cgttcttgtc ccgccaggaa
      181 agaacttccc ggaggatgcc ggccgccaag gccacggtgt cgccgttttc gcgacggagg
      241 agccgcatga gcttgccttc gttggtgtcc tggtccacga cgggcccctc ttgaaacgct
      301 ttcgcgttca acagttccgt caggccgacg cccaacccct cggcgacttt ctgcatcgtg
      361 gtcaaggtcg ggtttcgttc cccgcgctcc aggccgccga tgtaggtcca gtgcaaaccg
      421 catttgtccg ccatttcctc ttgggtgagg ccctggcgtt tgcggaggct ccgaattcgc
      481 aatcccaact cttttttggc gttcacggtg gtcgacatgg ctgaaaatat acattacacg
      541 attggaagca acaacagaac ggcgtgtgtt taataggcga atcgatgaat tctaagagta
      601 tttttgacct ttttttcgtt cgttctcttt tgccgtgaga acgaaatggg ggcagaattt
      661 tctggcccat cctcccacga tgcggcgcat tgcggaggcc ctggaactcg cgccgggcga
      721 cgccgttttg gagatcggcc cggggcgggg ggccttgacc caatgcctat cggaaaaagc
      781 cggtcgtctc gtcgcggtcg agctcgaccg cgatttggcc gtccgccttc ccgcgcgttt
      841 tcccaaggcg gaaatcgtca acgccgattt tttggactgg ccgccgccgg aggtcccggc
      901 ggggacgttt cgggtgatcg gcaacctgcc ctattcggcg gccgccccca ttcttcagaa
      961 ggtgctcgcc tggacgggtt gggaccgggc ggtgttcatg gtgcaaaagg aagtggccga
     1021 tcgcatcgcc gcccccgccg gcaccaaagc ttttggaatt ctttccctgg cggttcaatc
     1081 ccgggctacc acggaaacgt tgttcgacgt gccgccgggg gccttcaatc cgcccccgcg
     1141 ggtgacctcc acggttctcc gcttccgtcg acgccccgtt ccgttgtttc gggacgaggc
     1201 cgcttttttt aacgtggtga aggccgggtt cgcccaacgg cggaaaacat tggcgaataa
     1261 tttggcggga tcgttttcga aagatcggga agcggtggaa aaaatcctga cccgttgcgg
     1321 ggtggatccc cgggcgcggg ccgaagtggt ttctctgccc caatgggtcg cgctggccga
     1381 ggcgatcggt tcctagggtc gaggc
//
LOCUS       DAOQLS010000625         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_76864_length_1405_cov_2.322963, whole genome shotgun sequence.
ACCESSION   DAOQLS010000625 DAOQLS010000000
VERSION     DAOQLS010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76864_length_1405_cov_2.322963"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PK362_10080"
     CDS             complement(<1..1177)
                     /locus_tag="PK362_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75216.1"
                     /translation="MVLNEKIHFGTKTDSNGLMQYAVLSRDVFVTVNGKRESTPRHVE
                     AGGALHLGLGESAVIDGKKYQGADRSIYDEMGNKLADIVGGDIKMNSWGRVTGTEGGI
                     SSPWLTQSLPDTLFSAFDSGVEYVKENPWTSAAMVVTTAAAFFIPVTGLAGLAIAAEK
                     GSKLASVASFALTAYTFENRFVTVLNTVMLTQGIASSGGDLGKISTSLVLAFAPGAAL
                     KGTELVGKMTAPVWVSNMLSMTKTEASVVGFNALREVGVSATLAARGGELFGTAATRT
                     IAFLTSAPVTGTAATIGRLALPFRSGLETLTVAASKSPAVKAIVESSYGEVITKVAAP
                     MIKNYALMEFVAKPLINAYTSGNTPQWANSVVEMYVFMPLVAFGLRPAGAAVGNVASG
                     "
BASE COUNT          299 a          416 c          405 g          285 t
ORIGIN      
        1 ccccggatgc aacgtttcct accgccgccc cggcaggacg aagaccgaag gctaccaacg
       61 gcatgaaaac atacatttca acgacgctgt tggcccattg aggcgtattt ccggaggtgt
      121 aggcgttgat aagaggtttc gcaacgaact ccatcagcgc gtagttttta atcatcggcg
      181 cggcgacttt cgtaatcact tcgccgtagc tcgattcgac gatcgccttg acagcgggcg
      241 atttcgaggc agcgacggtg agcgtctcaa ggcccgatcg gaagggcaac gcgaggcgcc
      301 caatggtggc cgctgttcct gtcaccgggg cgctggtcaa gaaagcaatg gttcgagtgg
      361 ccgcggtccc aaaaagttca ccgccccgcg cggcgagagt ggcggagact ccgacttccc
      421 tcaaggcgtt aaagccgaca acgctcgctt cggttttggt catggaaagc atattggaaa
      481 cccaaaccgg cgcggtcatt ttgccgacca attccgtgcc cttcagcgcg gctcccggag
      541 caaaggcgag tacgagagaa gtggaaattt ttccgaggtc tcccccgcta gaggcgattc
      601 cctgagtcaa catcaccgtg ttgagcaccg tcacgaaacg attttcaaag gtgtaggcgg
      661 tcagggcaaa gctggcaacg ctggccaatt tcgacccttt ttcagcggca atcgcgagac
      721 cggccaatcc ggtcacgggg atgaagaacg ccgcggcggt ggtgacgacc atggcggcgc
      781 tcgtccaggg gttttcctta acgtattcga caccggaatc gaaagcggaa aagagggtgt
      841 ccggcaggct ttgcgtcaac caaggagagg agatgccgcc ctcggtgccg gtaacgcggc
      901 cccaggaatt catcttgatg tcccctccga cgatgtccgc caatttgttc cccatttcat
      961 cgtaaatcga ccggtcggcg ccctgatact ttttaccgtc gatcaccgcg gattcgccca
     1021 atccaagatg cagagccccg ccggcttcga cgtgacgcgg agtggattcg cgtttgccgt
     1081 tcaccgtgac aaagacgtcg cgcgacaaaa ccgcgtattg catcaggccg ttgctgtcgg
     1141 tcttggttcc gaagtggatt ttctcattga gaaccacgtc gccgatggaa gaaatggagt
     1201 aatcttcccg aatgttgccc ggtttctcga cttgcgctcg aaccggcatg ttcttggaat
     1261 cggtttgaac cacccgaaca ttggtgacgt cgcgctcgac gccgttgaca aaccgaacaa
     1321 accgcgttcc ggtggccccc atggccccca aaacgccggc cgcgctatcg tctttgacgt
     1381 ccaacgaccg gacgcttagg gcgcc
//
LOCUS       DAOQLS010000626         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_76920_length_1404_cov_3.465530, whole genome shotgun sequence.
ACCESSION   DAOQLS010000626 DAOQLS010000000
VERSION     DAOQLS010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76920_length_1404_cov_3.465530"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..576
                     /locus_tag="PK362_10085"
     CDS             136..576
                     /locus_tag="PK362_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002481615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNC75217.1"
                     /translation="MTDDLYRDLILDHFRHPRNQGALEKPDVKVQGVNPLCGDNLELT
                     FIVREGKIEDIKMTGNGCSISQSSASMMTEALKGKDLSTSQSLAQAFKKMMLQNGSAD
                     DLPEDLEDLAALEGVQKYPVRVKCALLAWNTLLQGLNDYQGKKA"
     gene            592..975
                     /locus_tag="PK362_10090"
     CDS             592..975
                     /locus_tag="PK362_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNC75218.1"
                     /translation="MPTIPEIRTVLKTVYDPEIHLSVVDLGLIYDIGVEKDGDKGERV
                     NVKMTLTTPACPYGPALLSKVHSELSNMTGVSDVKVDLAWIPPWDPRTMASDEAKLKM
                     GLFELDDADETETAETPGETPKTKV"
BASE COUNT          303 a          402 c          399 g          300 t
ORIGIN      
        1 ctgccccggc ggggaccggg tccaggacgt ctatgttttt aaaaacaccc tggccgtttt
       61 cttgaacgtg tacacggccg ggttcgaggg accggacatg cgctttttag ccctgggcgc
      121 ccccttggag acgaaatgac cgacgacctt taccgcgatt tgattttgga ccacttccgg
      181 cacccccgca accagggggc gctggagaag cccgacgtca aagtccaggg cgtgaacccc
      241 ctttgcggcg acaacctgga gctgaccttc atcgtccggg aaggcaaaat cgaggacatc
      301 aagatgaccg ggaacggctg ttccatttcc cagtcctcgg cctccatgat gaccgaggcg
      361 ctcaagggga aggatttgtc cacctcccaa tccctggccc aggcctttaa aaagatgatg
      421 cttcaaaacg gctccgccga cgacctgccc gaggatttgg aagacctggc ggccctggag
      481 ggcgtgcaga aataccccgt gcgggtgaaa tgcgcgctcc tggcctggaa caccctgctc
      541 caggggttga acgactacca gggaaagaaa gcctgattta aggagacgcc cgtgcccacg
      601 attcccgaaa tccgcaccgt cctaaaaacc gtttacgatc ccgaaatcca tttgagcgtg
      661 gtggacctgg gcctgatcta cgacatcggg gtggaaaaag acggcgacaa gggcgagcgc
      721 gtgaacgtca agatgaccct caccacgccg gcctgtccct acggccccgc gctcttgagc
      781 aaggtccatt cggaactctc caacatgacg ggcgtctccg acgtgaaagt cgacctggcc
      841 tggatcccgc cctgggaccc ccgcaccatg gcctccgacg aagccaagct caagatgggc
      901 ctcttcgagt tggacgacgc ggacgaaacg gaaaccgcgg agacgcccgg agaaacgccg
      961 aaaacaaagg tctaacgccg tgtggggccg gacgggtttt attataagtt tggtcatcgg
     1021 ccttctggag ggcgtcctcc tccttcaggg agggatgcgg gcggaaacca cgacgttcac
     1081 atccggcttg ttcctatttc taccttggtt gcttccgatc acgggcgtcc ttttttggat
     1141 ttccaacaaa gggaaccccc gcggggatcg aaccgctttt cttcgcggcg cttttttcgg
     1201 gttttgtctc gtttttggcg tcccgtggat tctgaagatt tttcgtttgg tcgagactct
     1261 tttctaagag cgaaccggtc cccgggggtt ttaagaatcc tgggaattgg tataatggga
     1321 agagtcaaca aatttgccgt attaagttct ttttaatcaa gatttaattt atcgagtgga
     1381 taaaagacga agggcgccct tgcc
//
LOCUS       DAOQLS010000627         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_76991_length_1403_cov_4.453264, whole genome shotgun sequence.
ACCESSION   DAOQLS010000627 DAOQLS010000000
VERSION     DAOQLS010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76991_length_1403_cov_4.453264"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1403
                     /locus_tag="PK362_10095"
     CDS             <1..>1403
                     /locus_tag="PK362_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC75219.1"
                     /translation="TIEKHAGDLANIYADLLINNRLQREAGLRMADVYRALSRPSSKP
                     GPLWTLYMRIYELLWGLNRGDLATGQITSALEGDAQWGARLFRAYAQDWVRGSGRFGA
                     LCFPYLLNDRTGELEKILSYFHDTRDAAAGGGVPAGLAEMDPDEEGESAHPSEDPALG
                     GVPRKKGTASTAQGGGDGQYREPFEYGQILKSMGVTLSDHDIAVAYYKERALRHLIPF
                     PSRKAPVGKDPLPEGSDAWDTGESLAAIDWSETLMTSPRVIPGVTTRQRVYGTMEGTP
                     PAPEPLDLDLYVDCSGSMPNPQTQMSYLTLAGTIVALSALRAGARVQATLWSGPGQFE
                     TTGGFVRDENRILRILTGYLGGSTAFPISLLGSTYRDRKPTDRPAHVLVISDDGVTTM
                     FGPDEWRRSGLKIAAEALALARGGGTWCLNLFGRDASGTEYDQARNMGWTIFTVKDWA
                     DLVAFAQSFGRTHYEKA"
BASE COUNT          244 a          466 c          454 g          239 t
ORIGIN      
        1 caccattgaa aaacacgcgg gggacctggc gaacatctac gccgaccttc tgatcaacaa
       61 ccggcttcag cgggaggcgg gcctccgcat ggccgacgtt taccgcgcgc tgtcccgccc
      121 gtcgagcaaa cccggccccc tctggacgct ttacatgcgg atctatgaac tgctctgggg
      181 attgaaccgg ggggatttgg ccaccggaca aatcacctcc gccctggaag gcgacgccca
      241 atggggcgcg cgactctttc gggcctacgc ccaggattgg gtgcggggga gcggccggtt
      301 cggcgccctg tgctttccct atctgctgaa cgatcggacc ggcgagttgg aaaaaatcct
      361 ttcttatttt cacgacaccc gggacgccgc cgccggcggc ggggtgcccg cgggcctggc
      421 cgaaatggac cccgacgaag agggcgagtc cgcccatccg tcggaagatc cggccctggg
      481 cggcgttccc cgaaagaagg ggacggcctc caccgcccag ggcggcgggg acggccaata
      541 ccgggaaccc ttcgaatacg ggcagatttt aaaatccatg ggcgtgaccc tttcggacca
      601 cgacatcgcc gtggcctatt acaaggagcg ggccctgcgg cacttgatcc cctttccgtc
      661 gcgaaaggcc cccgtgggga aggaccccct gcccgagggg tccgacgcct gggacacggg
      721 ggagtccctg gccgccatcg attggtcgga aacgctcatg acgagccccc gggtcattcc
      781 cggcgtgacc acccgccagc gggtctacgg caccatggag ggcactccac ccgcgccgga
      841 gcccctggac ctggacctct acgtcgattg ctcgggctcc atgccgaacc cccagaccca
      901 aatgtcctat ttgaccctgg ccgggaccat cgtggcgctc tcggctctgc gggcgggggc
      961 ccgggtgcag gccacgcttt ggagcgggcc cgggcaattc gaaaccaccg gggggtttgt
     1021 ccgggacgaa aatcgaattc tgcgaatctt gacggggtat ctgggcgggt ccacggcttt
     1081 tcccatttcc ctcctgggaa gcacctaccg ggaccgaaaa cccacggacc ggcccgccca
     1141 cgtcctggtt atttccgatg atggcgtgac caccatgttc ggtccggacg agtggcgtcg
     1201 gtcgggcctt aaaatcgccg ccgaggcgtt ggccttggcc cggggcgggg gcacctggtg
     1261 cttgaatttg ttcggacgcg acgcttccgg caccgagtac gaccaggccc ggaacatggg
     1321 gtggacgatc ttcaccgtca aagattgggc ggatctcgtg gcctttgccc agtccttcgg
     1381 ccgaacccat tacgaaaagg cgg
//
LOCUS       DAOQLS010000628         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77116_length_1402_cov_3.549369, whole genome shotgun sequence.
ACCESSION   DAOQLS010000628 DAOQLS010000000
VERSION     DAOQLS010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77116_length_1402_cov_3.549369"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1402)
                     /locus_tag="PK362_10100"
     CDS             complement(<1..>1402)
                     /locus_tag="PK362_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNC75220.1"
                     /translation="TEGGGTDAASLSALLSLPETRLMEVLGNRLASAVPPPSEADLAP
                     FSPWGLALGDLGVALTRTEGRWILELLLSALLTSPRAFGGDPVAPVDEAVIQRTVHET
                     TRDWTNEAFLSFLEPGFQRVGDGPRALREAFLARIPPWAYPEEIRKAWGGPVLPVEDS
                     PERVAEAAQREALALGRIVADRLVELQADAPRTATTKPDGTKVSRADEEAMVTLASGI
                     LEAFPTHEIIGEEDLEPLAPELHRRNRANAGSPYQWFIDPLDGTETYLGGTDIFAVHL
                     ELQHEGRPLLAVVVLPRKGPDGGPLVVMARAGRAGLWVNGQWHAPRSPEEFPTDFAQM
                     TAVAHGKPATGEPYPFFQRVRALFGETVPRVGSSGLWLTGLALRRVGLSVPGLPDNYS
                     YYASQRVKSWDVAAPGFLLENAGGVLEVHDHGRPFFPLHQRYEDKEAKFSVLAALTPE
                     GAAAWRRAVLESASGDG"
BASE COUNT          247 a          476 c          467 g          212 t
ORIGIN      
        1 ccccgtcgcc ggaggcggat tccagcacgg cgcgtctcca ggcggcggcg ccctccgggg
       61 tgagggcggc caacacgctg aatttggcct ccttgtcctc atacctttga tggagcggga
      121 aaaagggccg tccgtggtcg tgaacttcga ggacgccccc ggcgttctcg agtaaaaagc
      181 ccggcgccgc cacgtcccaa ctcttaacgc gttgggaggc gtaatacgaa tagttgtccg
      241 gaagtcccgg caccgacagg ccgacgcggc ggagggccag ccccgtgagc cagagcccgc
      301 tcgaaccaac ccgggggacg gtttccccaa agagggcgcg gacccgttgg aagaacgggt
      361 agggttcgcc ggtggcgggt tttccgtggg caacggccgt catttgggcg aaatccgtcg
      421 gaaattcttc gggggagcgc ggggcgtgcc attggccgtt gacccacaat cccgcgcgcc
      481 cggcccgggc catgaccacg aggggcccgc cgtccggccc cttccgggga aggacgacca
      541 ccgccaggag gggccggccc tcgtgttgaa gctcgagatg caccgcaaag atgtcggttc
      601 cgcccagata cgtttccgtg ccgtccaggg gatcgataaa ccactggtac ggcgacccgg
      661 cgttggcgcg atttcgtcga tgcaattccg gggcgagggg ctccaaatcc tcctccccga
      721 tgatttcgtg ggttggaaag gcctcgagaa ttccggaggc cagggtcacc atggcctcct
      781 cgtcggcccg ggacaccttc gttccatcgg gtttggtggt cgcggtgcgg ggggcgtcgg
      841 cctgcaattc caccaaccgg tccgccacga tccggcccag cgccagggct tcccgctggg
      901 ccgcctcggc cacccgttcc ggggaatcct ccaccgggag aaccggaccg ccccaggctt
      961 tccgaatttc ctcgggataa gcccacgggg ggattcgggc gagaaacgct tcgcgaaggg
     1021 cccggggacc gtccccgacc cgctggaatc ccggttccaa aaaggaaaga aacgcctcgt
     1081 tcgtccaatc ccgggtcgtt tcgtgaaccg tccgctggat cacggcctcg tccaccgggg
     1141 cgacggggtc cccgccgaag gcccggggag aggtcaacag ggcactcaac aaaagctcga
     1201 gaatccagcg cccttccgtc cgcgtcaagg ccacgcccaa atcgcccaag gccagtcccc
     1261 agggcgaaaa gggcgccagg tccgcctcgg aaggaggcgg cacggcggac gccaaccgat
     1321 tccccagaac ctccatcaga cgggtttcgg gcaacgacaa aagggcgctg aggctcgcgg
     1381 cgtcggttcc gccgccctcc gt
//
LOCUS       DAOQLS010000629         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77233_length_1401_cov_3.365527, whole genome shotgun sequence.
ACCESSION   DAOQLS010000629 DAOQLS010000000
VERSION     DAOQLS010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77233_length_1401_cov_3.365527"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1401)
                     /locus_tag="PK362_10105"
     CDS             complement(<1..>1401)
                     /locus_tag="PK362_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75221.1"
                     /translation="LEALRDRLAHQCPAVLSGLAWYRESDAGTRAAAAEQWSDLFLKY
                     FFTGDLRRAGIHLVRRADTTSTYGLMASSLSEPESALWARSNQPFYLWMSDDGRSVAG
                     SSESTAFLGGRSGDSPFAHRWTLANGEIAFLSGARLTIDHVTKGRVAEFDLNDPAAVF
                     AHRRWLDLRNSRRVPPAADAGDSPTTRVRDDFNLIPKVEKAIAADFGKRSSDNAKTGR
                     AFAELLLNRLLARETDDGGLDLVVVGTEKSFDEGKTFAELLEKISSLAGRRLRTETVY
                     GADFTREDLIRMRDRGFSERTVVLGLASSGQTANTFYTLENLFEAWRAMTENARGDPA
                     ATPPHFLVSSHMDNPYTEELLGQGLGDGDPFKERNFVTFPAPLDPFHPAEAATVTHKA
                     AERLLKEVALILARRLASQRGWAGSRLTSKTVSLFRRMVDNGDELNRRIVGLDEDGNP
                     HLNSRGEPNDIPRQIQA"
BASE COUNT          261 a          446 c          433 g          261 t
ORIGIN      
        1 ggcctgaatc tggcgcggga tgtcgttggg ttccccccgg ctgttgagat ggggattccc
       61 gtcttcgtcc aggccgacga ttcggcggtt caattcgtcg ccgttgtcca ccatgcgtcg
      121 aaacaacgaa acggtcttgg aagtcaaacg cgaaccggcc catccccgtt gggaggccag
      181 gcgccgcgcc aagatcaacg ccacttcctt cagcaatcgt tcggccgcct tgtgagtcac
      241 cgtcgcggcc tcggccgggt ggaacggatc gagcggggcc ggaaacgtta cgaaattgcg
      301 ttctttgaaa gggtccccgt cgcccaatcc ctgtcccaac aattcctcgg tgtacggatt
      361 gtccatgtgc gaagacacga ggaaatgggg cggggtggcg gccggatcgc cccgggcgtt
      421 ttcggtcatc gcgcgccaag cttcgaacag gttttccaag gtgtaaaaag tgttggcggt
      481 ttgacccgaa ctggccaggc ccaagaccac cgtccgttcg ctgaaccccc ggtcgcgcat
      541 gcggattaaa tcctcccggg tgaaatcggc gccatagacg gtttccgtcc gcagccggcg
      601 gccggccaaa gaggatattt tttccaaaag ttccgcgaag gtcttccctt catcgaagct
      661 cttttccgtc ccgaccacca ccaaatccag cccgccgtcg tcggtctccc gcgcgagaag
      721 tcggttcagc aaaagttccg cgaaagccct tccggttttc gcgttgtccg acgatcgttt
      781 cccgaagtcc gccgcaatgg ctttttcgac tttgggaatg aggttaaaat cgtcccgaac
      841 gcgcgttgtc ggggaatccc cggcgtcggc cgcgggcggg acgcggcggc tgttccgaag
      901 atccagccag cgccgatggg cgaacacggc cgccggatcg ttaaggtcaa actccgccac
      961 ccgccccttg gtcacgtgat cgatcgtcag gcgcgcaccg gacaggaagg cgatttcacc
     1021 gttggcgagg gtccatcgat gggcgaaggg gctgtcgccg cttcgaccgc ccagaaacgc
     1081 cgtggattcg ctgctgccgg ccacgctgcg accgtcgtcc gacatccaca agtaaaaggg
     1141 ctggttggag cgggcccaaa gggcgctttc cggctccgac aacgaagacg ccatgaggcc
     1201 gtaggtggag gtcgtgtccg ctcgacgaac gaggtggatc ccggcccgtc gcaaatcccc
     1261 ggtgaaaaaa tatttaagga acaggtcgga ccattgttcc gcggcggcgg cgcgcgtccc
     1321 ggcgtccgat tcgcgatacc acgccagccc actcaacacc gccgggcatt gatgggccaa
     1381 ccggtcgcgc agggcctcca g
//
LOCUS       DAOQLS010000630         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77270_length_1401_cov_2.526746, whole genome shotgun sequence.
ACCESSION   DAOQLS010000630 DAOQLS010000000
VERSION     DAOQLS010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77270_length_1401_cov_2.526746"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1401
                     /gene="cobA"
                     /locus_tag="PK362_10110"
     CDS             <1..>1401
                     /gene="cobA"
                     /locus_tag="PK362_10110"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNC75222.1"
                     /translation="RLLDHCRKGATVIDAGKRGHGRVLMRQGEINRLLVRRARAGERV
                     VRLKGGDPYLFGRGGEEAEFLARARVPFEVVPGVSSLGAVPASAGIPLTHRGLASTVT
                     VVTGHGSDPNPYLRESRQERDVRRAPPVAWERIPRDGTLVVLMGLGRLPEIVRELRRA
                     GWAADTPAAVIASGTLPVQRSVRGALSDIAGRARAAGLRPPATVVFGRVVSLAAGLNW
                     FERRPLFGWTVLVTRAEGQSSGLKDLLEEQGARVVDAPAIKIDPLPLPADVRRALRHL
                     TDFDGVLFTSANAVRAAAAHLPGPWPASVPVYAVGPKTAEAIRRAGWRVARVAAEFRA
                     EGLARVLGKKLRGKTYLFPRAARGRDLLIDFLSKAGARTLLWPVYRTRLLPPTADLRA
                     RLRRGEFDAVTFTSSSTVHGLLGRMARGERARIFKKTRAASIGPLTTAALRSLGVRPA
                     VTAPGATVDSLVGALVR"
BASE COUNT          170 a          465 c          516 g          250 t
ORIGIN      
        1 cggttgctgg accattgtcg aaaaggggcg acggtcatcg acgccggcaa acggggccac
       61 ggccgggtgc tgatgcgcca gggggagatc aatcggttgc tggttcggcg cgcccgggcg
      121 ggcgagcggg tggtgcgcct gaaaggcgga gacccctatc tcttcggccg ggggggcgaa
      181 gaagccgaat ttttggcccg ggcccgggtt ccctttgaag tggttcccgg tgtttcctcc
      241 ctcggcgctg tccccgcttc ggcgggaatt cccctcacgc accgggggct cgcctcgacc
      301 gtcaccgtcg tcacgggcca cgggagcgat cccaacccct atttgcgcga atcccgccag
      361 gagcgagacg tccgtcgagc cccgccggtg gcctgggagc gcatcccccg ggacggcacc
      421 ttggtggtcc tcatgggttt gggccgtctg ccggaaatcg ttcgggaatt gcgccgggcg
      481 ggatgggccg ccgacacccc ggcggcggtg atcgcctcgg ggacgttgcc ggtccagcga
      541 tcggttcggg gggccctgtc cgacatcgcc gggcgggccc gggcggccgg attgcggccg
      601 cccgccaccg tcgtgttcgg gcgcgtggtc tctctggcgg ccgggcttaa ctggtttgag
      661 cgtcgccccc ttttcggatg gacggtcctg gtgacccggg ccgaggggca atcgagcggt
      721 ttgaaggacc tgttggagga acaaggggcc cgggtggtcg acgccccggc cattaaaatc
      781 gatccgctgc cgctccccgc cgacgttcgt cgggcgttgc gacatttgac cgattttgac
      841 ggcgtgcttt tcaccagcgc caacgcggtt cgggccgccg cggcccacct tcccggtccc
      901 tggccggcgt ccgtgccggt ttacgcggtg gggcccaaaa ccgccgaggc gatccggcgg
      961 gcgggttggc gggtggcccg ggtggccgcg gaattccggg cggagggatt ggcccgggtc
     1021 ttgggaaaaa agcttcgtgg gaaaacctat ctttttcccc gcgcggcccg ggggcgggac
     1081 ctgttgatcg attttctctc gaaggccggc gcccggacgc ttctttggcc cgtgtaccga
     1141 acgcgccttc ttccgcccac ggccgatctt cgggcccgat tgcgccgggg ggaattcgac
     1201 gcggtgacgt tcacgtcctc ctccacggtc catggcttgt tgggccgaat ggcgcgcggg
     1261 gaacgggccc ggattttcaa gaaaacccgg gcggcctcca tcgggcccct cacaaccgcc
     1321 gcgcttcgct ccctgggcgt tcgcccggcg gtgaccgcgc cgggggcgac ggtggactcc
     1381 ctggtgggcg ccctggtccg g
//
LOCUS       DAOQLS010000631         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77345_length_1400_cov_3.298885, whole genome shotgun sequence.
ACCESSION   DAOQLS010000631 DAOQLS010000000
VERSION     DAOQLS010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77345_length_1400_cov_3.298885"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK362_10115"
     CDS             complement(<1..747)
                     /locus_tag="PK362_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75223.1"
                     /translation="MKIDRRWAVLAWLTTAAMTSHGAEEDAVAVDERRLEDGDDGEYP
                     EFPAWSGDWRIRTREVYPPGDTYRNAPGSLRHNRYVSARTRLRRGAVSATALAKRAAV
                     GPALSYDAMREWGTLKAAVAVGVDGTGPSLVAGDYAMSFGQGLVFYDGFGEFVRPARV
                     RERRPRPDTSPSPEEYFRGAALGAPLGPFRVDLFVSQKALGLPLNPDGTVDFDWDDFH
                     DRSGDVQTAQDLLHHDSVTERMAGGRLSGRW"
     gene            complement(744..>1400)
                     /locus_tag="PK362_10120"
     CDS             complement(744..>1400)
                     /locus_tag="PK362_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75224.1"
                     /translation="VGLGHTDLLGMPEMPRNTVSAVAPGRGRVGWGMYGVDFGSSLYR
                     EREVGLAAGGSLDPSSALGVILKGQQMSVERYGRAAAWQMDVGWLGRPVPLLGLGFVV
                     RNATQSRFRGLPDKSAAVFQAGARFDFWNGAPAFLALRRGADGSGDWRWGQEILIRSS
                     AIFRMGYCSSPARWALGWGFAFSQWSMDYAFLTHPKLPDQHQFNLTWRGPRRLRSPSK
                     "
BASE COUNT          272 a          511 c          425 g          192 t
ORIGIN      
        1 ccaccgaccg gagagccgcc ctcccgccat cctttccgtg acggagtcgt ggtggagaag
       61 gtcctgggcc gtttgaacgt cccccgaccg atcgtgaaaa tcgtcccaat cgaagtccac
      121 cgttccgtcg gggttcaggg gcaatcccag cgctttttgg gaaacgaaca aatcgacgcg
      181 aaacgggccg agaggggccc cgagggccgc gccgcggaaa tactcctccg gggaaggcga
      241 cgtgtcgggc cggggacgcc gctcccggac ccgcgcgggc cgcacgaact ctccgaaccc
      301 gtcgtaaaaa acgagtccct gcccgaaact catggcgtaa tcccccgcca ccaaagaggg
      361 tccggtcccg tccactccaa cggcgaccgc cgccttgaga gtgccccatt cccgcatcgc
      421 gtcgtacgaa agcgccgggc ccacggcggc gcgcttggcc agggcggtgg ccgacaccgc
      481 gccccggcga aggcgggtgc gggccgaaac gtaccgattg tggcggagag accccggcgc
      541 gttccgatag gtgtccccgg gcggatacac ttcccgggtt cgaatccgcc aatccccgga
      601 ccaggccggg aattcggggt actccccatc gtccccgtct tccaaccggc gttcatcgac
      661 ggcgacggcg tcctcctcgg ccccgtggga agtcatcgcc gccgtcgtta accaggcgag
      721 cacggcccaa cggcggtcga ttttcatttc gacggggacc gaaggcgcct ggggccccgc
      781 caggtgaggt taaactggtg ctggtccggc agtttcgggt gggtgaggaa cgcgtaatcc
      841 atggaccatt gggaaaaggc gaatccccac cccaaggccc atcgggccgg cgacgaacaa
      901 taccccatcc gaaaaatcgc cgatgatcga attaaaatct cctgacccca tcgccagtcc
      961 ccggacccgt ccgccccccg gcgaagggcg aggaacgccg gcgccccgtt ccagaaatcg
     1021 aacctcgccc ccgcctgaaa aacggccgcc gatttatccg gcaaaccccg aaagcgcgat
     1081 tgggtggcgt ttcgaaccac gaacccaaga cccaacaacg gaaccgggcg ccccagccac
     1141 ccgacatcca tctgccaggc cgcggcgcgg ccgtagcgct cgacggacat ttgctggccc
     1201 ttgaggatca cccccagggc ggaggaggga tcgagggacc cgcccgcggc caaacccact
     1261 tcccgctccc ggtacaagct tgaaccaaaa tcaacgccgt acatccccca accgacccgc
     1321 cccctccccg gggccaccgc cgacaccgtg ttccggggca tttccggcat tccgaggagg
     1381 tcggtgtgac cgaggccgac
//
LOCUS       DAOQLS010000632         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77489_length_1399_cov_2.770833, whole genome shotgun sequence.
ACCESSION   DAOQLS010000632 DAOQLS010000000
VERSION     DAOQLS010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77489_length_1399_cov_2.770833"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1399
                     /locus_tag="PK362_10125"
     CDS             <1..>1399
                     /locus_tag="PK362_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75225.1"
                     /translation="GELSLPWSAFEKLLQLNKDNVLLSWEEFQRLLQQTGVAEPPPYQ
                     LQNGQVSLTREEFKHLLDRLKPPAGEGAKAYMTKAIYTGRVTRKGATVTAKIRLDVAT
                     PVGAYSPVRVPLFPEGVAFEDISLDGRPALVESAGGWTTVTVPRPGEHELTAVFALSA
                     TLDKEPYRVHFQIPSTPITQLDFFLDAPHLEARVDGAAQAVSTPADGGTRVRASLPQG
                     NSIGLSWNAVEPERAKGPAKVYATVHQLLSVQDDAVRATARVELDVLQNTINNFSIVL
                     PEGYTLLDVTGDAVGEWKKRGEGERTLFVPFSYARKGRFTVDVRVERAFKDAGGALPF
                     DGFRVLDVVRESGDLLIEKTTNGEVTVAETAGLTRLDPRETPPALSALASQTYLDAYK
                     YIRPPYRLSLDIRRHEEIAVVSSVIDTANAVTLLLKDGKCVTHVTWSIKNTARQFLEL
                     RLPAGAEVWSVVVDGR"
BASE COUNT          259 a          482 c          425 g          233 t
ORIGIN      
        1 ggggaactga gcctcccgtg gtcggcgttt gaaaaactcc tgcagctcaa caaagacaac
       61 gtgcttctct cctgggagga atttcagcgg ctcctccagc aaaccggcgt cgccgagccc
      121 ccaccctatc aacttcagaa cggccaggtc tccctgacgc gggaggagtt caaacacctt
      181 ctggatcgcc tcaaaccccc cgcgggcgaa ggggccaagg cctacatgac caaggccatc
      241 tacacgggcc gcgtcacacg aaagggagcg accgtcaccg ccaaaatccg attggacgtc
      301 gccacgcccg tcggcgccta ttccccggtt cgggtgccgc tctttcccga aggcgttgct
      361 ttcgaggaca tctccctgga cgggcggccg gccctcgtgg agagcgccgg cggctggacc
      421 acggtcacgg taccccgccc cggcgagcac gaattgacgg ccgtgttcgc cctatcggcc
      481 accctcgaca aggaacccta ccgcgtccat ttccaaattc cctcgacgcc catcacgcag
      541 ctggactttt tcctggacgc gccccatttg gaggcccggg tggacggcgc cgcccaggcg
      601 gtctcgacgc cggccgacgg cggcacccgg gtgcgggcct ccctgcccca ggggaattcc
      661 atcgggcttt cctggaacgc cgtggaaccg gaacgggcca agggccccgc caaggtgtac
      721 gcgacggtcc atcaactgct ttccgtccag gacgacgccg tgcgcgccac ggcccgggtg
      781 gaattggacg tgctccaaaa caccatcaac aatttttcca tcgttcttcc cgaaggctac
      841 accctgttgg acgtgaccgg cgacgcggtg ggggaatgga aaaaacgcgg ggagggcgaa
      901 cgcacgctgt tcgttccctt ttcgtacgcc cggaaaggtc gttttacggt ggacgtccgg
      961 gtggaacgcg cctttaagga cgccggcggg gccctgcctt tcgacgggtt ccgggtcctg
     1021 gacgtcgtcc gcgaaagcgg cgacctgctg atcgaaaaaa ccacgaacgg cgaagtcacg
     1081 gtggcggaaa cggccggctt gacgcggctg gacccccggg aaacgccccc ggccctgagc
     1141 gccctggcct ctcaaaccta cctggacgct tacaaataca ttcggccgcc ctaccgtctg
     1201 agcttggaca ttcgacgtca cgaggagatc gcggtggttt cctcggtgat cgacacggcc
     1261 aacgccgtga ccctcctgct caaggacggc aaatgcgtca cgcacgtgac ctggtcgatc
     1321 aaaaacaccg cgcgccaatt tctggaactc cgcctcccgg ccggggccga agtttggtcg
     1381 gtggtcgtgg acggccgcc
//
LOCUS       DAOQLS010000633         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77636_length_1397_cov_7.248137, whole genome shotgun sequence.
ACCESSION   DAOQLS010000633 DAOQLS010000000
VERSION     DAOQLS010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77636_length_1397_cov_7.248137"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="PK362_10130"
     CDS             <1..1368
                     /locus_tag="PK362_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75226.1"
                     /translation="VRTGMMAALTDAERFPLLTPALAKTLKTYREHPAAPRFNYACGD
                     RLTAEGLKRVRAFEAQVKKRRSSPKGLPPWIEEKVDEWLKKVPFYRQRGGRAKDFETL
                     PAIGREDLLATPWAFVPDGRALDDLMVYETTGTTGAPLGVMYHPEVPSMYLVLLRSAL
                     KAAGVELKGGPGVALLLVGAQEKALTFVSGSTYLDGAGYVKVNLKPSDWRRPEDRATY
                     LSFCSPEVVTGDPVAFQELLDLKVPLRPKALVSTSLALAAGLRKKLESTLHCPVLDVY
                     SMTECRPLAASTGGDFEIIPPDVHAEILDDRGRPLPDGRRGEVAITGGRNDFLPLIRY
                     RTGDHAAMVRRGGRRFLKGLEGRAPVVYLGSEGRRVPSIDIARALGPLALSHYVLHQR
                     ADGSFLFKCPNPADHRSALRALLEKKLGSKIRLSVEKLAEEDRRGGKVIPHRSDVKNP
                     RGPLW"
BASE COUNT          232 a          462 c          461 g          242 t
ORIGIN      
        1 gtacggacgg gaatgatggc cgccctcacc gacgccgaac gatttccgct gttgacgccc
       61 gccctggcga agacgctcaa aacctaccgg gagcacccgg ccgctccgcg cttcaattac
      121 gcctgcggcg accgcctgac cgccgagggg ttgaagcgcg tgcgggcctt cgaggcccaa
      181 gtcaaaaaac gccgatcgtc gccgaagggc ctgccgccct ggatcgagga aaaggtcgac
      241 gagtggctga agaaggttcc cttttaccgc caacggggcg gccgggcgaa ggattttgaa
      301 accctgccgg cgatcgggcg ggaggacctg ttggcgacgc cctgggcctt cgtcccggac
      361 ggccgggcct tggacgacct gatggtttac gaaaccaccg gcaccaccgg cgcgcccctg
      421 ggggtgatgt accaccccga agtgccgtcc atgtatttgg tgttgcttcg gtcggcgctc
      481 aaggccgcgg gcgtggaact taagggcggg cccggcgtgg cccttctcct ggtcggcgcc
      541 caggagaaag ccctgacctt tgtgagcggt tccacctatt tggacggcgc ggggtacgtc
      601 aaagtgaatt tgaaaccctc cgactggcgg cggcccgaag accgggccac gtatctttcc
      661 ttttgttcgc ccgaggtcgt caccggggac cccgtggcct tccaggagtt gctggattta
      721 aaagtgccgc tccggcccaa ggccctggtg agcacctccc tggcgctggc ggcgggcctg
      781 cggaaaaaat tggaatcgac gctccattgc cccgtgctgg acgtctattc catgacggag
      841 tgccgccccc tggcggcctc caccggcggc gactttgaaa tcattccccc ggacgtccac
      901 gcggagattc tggacgaccg gggccgcccc ctccccgacg gccgccgggg ggaagtggcg
      961 atcacgggcg gccggaacga ttttctcccc ttgatccgtt accgcaccgg ggaccacgcc
     1021 gcgatggttc ggcggggcgg gcgccggttt ctgaagggat tggagggccg ggccccggtg
     1081 gtgtatctcg ggtccgaggg gcggcgggtg ccctccatcg acatcgcccg ggccctgggg
     1141 cctctggccc tttcccacta cgtcctgcac cagcgggccg acgggtcctt cctatttaaa
     1201 tgcccgaacc cggcggacca ccgctcagct ctgagggctc tgttggaaaa gaaacttggg
     1261 tcaaagatcc gattgtccgt tgaaaaactg gccgaggagg accgccgggg gggcaaagtg
     1321 attccccatc gctccgacgt caaaaacccc cggggccccc tgtggtaaaa tccccgacca
     1381 tgacggcccc ggctttg
//
LOCUS       DAOQLS010000634         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77703_length_1397_cov_2.228018, whole genome shotgun sequence.
ACCESSION   DAOQLS010000634 DAOQLS010000000
VERSION     DAOQLS010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77703_length_1397_cov_2.228018"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /gene="asnB"
                     /locus_tag="PK362_10135"
     CDS             complement(<1..1331)
                     /gene="asnB"
                     /locus_tag="PK362_10135"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNC75227.1"
                     /translation="MGRRGPDHAAWKSWSGPRGVVELGHTRLSIIDLDPRSHQPFYRE
                     GLWVAFNGEIYNHVELRDALVREGLRFETTSDTEVLLALWRREGPAALDRLEGMWAFA
                     MYDERDGSLTLARDRFGEKPLFLWDDGTDLYFASEVKGLAALRGAWPRVNGDQVRRFL
                     VNGYKSLYKGTDTFFQDVRSLPAGHWRRRTADGKTTEGAYWAPRPAPEKDMTFEEAVA
                     GTRERLFRSVEWRLRADVPLAFCLSGGVDSNALIAVAKRRLGFDAHGFTVANTDPRYE
                     ERDVIEAAVADLKVRHTWVAARTEGFLENLRRLVDYHDAPVYTISYYLHGLLMEAVRR
                     AGYKVSISGTGADELFTGYFDHHLFYLAERRGDPAGAAEARANWEREVKPYVRNPFLK
                     DPDRIAADPSFRDHIFLDADKFAAYLRTPWREAFAEERYTANVLRNRMLNE"
BASE COUNT          236 a          496 c          449 g          216 t
ORIGIN      
        1 aactcgttga gcattcgatt gcgcaggacg ttcgccgtgt aacgctcctc cgcgaaggcc
       61 tcccgccagg gcgttcgaag ataggcggcg aacttgtccg cgtccagaaa aatatggtcc
      121 cgaaagcttg gatcggccgc gatccggtcc gggtccttca aaaacgggtt ccgcacgtag
      181 ggtttcactt cccgctccca gttcgcccgg gcctccgccg cgcccgccgg atcgccccgc
      241 cgctcggcca ggtagaacag gtggtggtcg aagtaccccg tgaacaattc gtcggccccc
      301 gtgccgctga tcgacacctt gtagccggcc cggcgcaccg cctccatcag caacccgtgg
      361 aggtagtagg agatggtgta caccggggcg tcgtggtagt cgaccagccg gcgcaggttc
      421 tccaggaacc cctccgtccg cgccgccacc caggtgtggc ggaccttcaa atccgccacg
      481 gccgcctcga tcacgtcgcg ttcctcgtag cgcgggtccg tgttcgcgac ggtgaatccg
      541 tgggcgtcga aaccgaggcg ccgtttggcc acggcgatca acgcgttcga atcgacgccc
      601 ccgctgaggc aaaaagccag cggcacgtcg gcgcgcaggc gccactccac cgaacggaaa
      661 aggcgctccc gcgtgcccgc cacggcctcc tcaaaggtca tgtctttttc cggcgcgggc
      721 cgcggcgccc aataggcgcc ttcggtcgtt ttcccgtccg cggtccgccg ccgccaatgg
      781 ccggccggca gggagcgaac gtcctggaaa aaggtgtccg tgcctttgta gagcgatttg
      841 tacccgttca ccaggaaacg gcgcacctgg tcgccgttga cgcggggcca ggccccccgc
      901 agggccgcca gccccttcac ttcggaggcg aaatacaaat cggtcccgtc gtcccacagg
      961 aacaggggtt tttcgccgaa ccgatcgcgg gcgagggtca aggacccgtc gcgctcgtcg
     1021 tacatcgcga aagcccacat gccttccagg cggtcgaggg cggccggacc ttcccgccgc
     1081 cagagggcca gcaagacctc ggtgtcggag gtcgtctcga aacggaggcc ctcccggacc
     1141 aacgcgtccc gaagctccac gtggttgtag atttcgccgt tgaaggcgac ccacagaccc
     1201 tcccgataaa agggctggtg ggagcgcgga tccaaatcga tgatgctcaa ccgcgtgtgg
     1261 cccagttcga ccacgccgcg gggtcccgac caggacttcc aggcggcgtg gtccggaccc
     1321 cgccgcccca accgctcgag gcacgatcgg gcggcggacg ccgcgacgcg cgcggggccg
     1381 gcgtgaccgg cgacgcc
//
LOCUS       DAOQLS010000635         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77858_length_1395_cov_3.577612, whole genome shotgun sequence.
ACCESSION   DAOQLS010000635 DAOQLS010000000
VERSION     DAOQLS010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77858_length_1395_cov_3.577612"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..574)
                     /locus_tag="PK362_10140"
     CDS             complement(80..574)
                     /locus_tag="PK362_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75228.1"
                     /translation="MNGAQWHLALIHFPVAGVFFALLLLTYAFFRKNGDVLKVALGFL
                     VVTGGAGLAAYWTGEGAEEVLEQATSVAGGWVHTHEEAAEAALIALEIAAALALGGLV
                     FFRRSGGPRPAYVGAVIGATILAAGLLGRAAHHGGQIRHTEVRPPSAADAPASKESPD
                     DHAH"
     gene            complement(639..>1395)
                     /locus_tag="PK362_10145"
     CDS             complement(639..>1395)
                     /locus_tag="PK362_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HNC75229.1"
                     /translation="ALQFLTGYVIEKSLSVDNMFVFIMIFSFFGIEAEHQPRVLKWGI
                     LGALILRFILIFVGATLVARFHWVLYVFGGILLYSAYKMAFGVEKEFNPDDNALFRRL
                     RRLMPMTGMAGQRFFVRLNGVLHATPLFLALAVVEFSDVVFALDSIPAIFSITQDTFI
                     VYTSNVFAILGLRALYFLVSGLVQLFAYLKYGVALILAFVGVKMLGSSYFHISTGVSL
                     GVILGVLAVSILLSLAFRRRPAPAAPDSASGHN"
BASE COUNT          328 a          430 c          440 g          197 t
ORIGIN      
        1 cagacgtcct tccccgtgaa cagccggcac caccaggtcg tgggccgggt cgccggcggc
       61 tttggggcgg gaggagccgt cagtgggcat ggtcgtcggg ggattccttg gaggccgggg
      121 cgtcggccgc cgacgggggg cgaacttccg tgtgtcggat ttgcccgccg tggtgggccg
      181 cgcggcccaa caggccggcg gcgaggatcg tcgcgccgat gaccgccccc acgtaggccg
      241 gccgggggcc gcccgaccga cggaaaaaga ccaggccgcc cagggccagg gcggcggcga
      301 tctccagggc gatcagggcc gcttcggcgg cctcttcgtg cgtgtgaacc cagccgcccg
      361 cgacggaggt cgcttgctcc aggacctctt cggccccttc cccggtccaa taagccgcca
      421 gcccggcccc gcccgtcacc accaggaacc cgagggccac tttcaacacg tcgccgttct
      481 tcctaaaaaa cgcgtaggtc aacagaagca gggcgaaaaa aacgcccgcc acgggaaaat
      541 ggatcaaagc caaatgccat tgagcgccgt tcatgggacc tccagggggt tgatcgctgt
      601 attttagccc gttcgcgaac gggcaaaaat gatctgggtc agttgtggcc ggaagccgaa
      661 tccggcgcgg cgggggccgg ccgtcgacgg aacgccaggg acaacaaaat ggaaaccgcg
      721 agcacgccca ggatcacccc cagggaaacc cccgtggaaa tgtgaaaata ggacgacccc
      781 aacattttca cgcccacgaa cgccagaatc aacgccacgc cgtatttcaa ataggcaaaa
      841 agttggacca acccgctgac caggaaataa agcgcccgca gacccagaat ggcgaagaca
      901 ttggacgtgt aaacaatgaa ggtgtcctgg gtgatggaaa aaatggccgg aatcgaatcc
      961 aaggcgaaaa ccacgtcgct gaattccacg accgcgaggg cgaggaacag cggcgtcgcg
     1021 tgcaggaccc cgttgaggcg gacaaagaac cgctggccgg ccattccggt catgggcatc
     1081 agccggcgca accggcggaa cagggcgttg tcgtcggggt tgaattcctt ctccacccca
     1141 aacgccatct tgtaggccga atacaggagg atcccgccga agacgtagag cacccagtga
     1201 aaccgggcga ccaaggtggc gccgacaaaa atcaaaatga agcgcaggat cagcgcgccc
     1261 aatatccccc atttcaatac gcgcggctga tgttcggcct cgatgccgaa aaaggaaaaa
     1321 atcatgatga aaacaaacat gttgtcgacg ctgagggatt tttcgatcac gtagccggtg
     1381 aggaactgca gcgcc
//
LOCUS       DAOQLS010000636         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_77972_length_1394_cov_3.253921, whole genome shotgun sequence.
ACCESSION   DAOQLS010000636 DAOQLS010000000
VERSION     DAOQLS010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77972_length_1394_cov_3.253921"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK362_10150"
     CDS             complement(<1..362)
                     /locus_tag="PK362_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNC75230.1"
                     /translation="MSVLVVGSVALDSVKTPFGQRAEALGGSAVYFSYSASFSTDVRL
                     VGVVGRDLPAKHLDLLRRRGIDLSGLQITDGETFRWSGYYEYDLNEAKTLDTRLNVFE
                     KFVPTLPESFRATDAVFLA"
     gene            complement(406..1098)
                     /locus_tag="PK362_10155"
     CDS             complement(406..1098)
                     /locus_tag="PK362_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75231.1"
                     /translation="MGLLDVKDLSFHFVESPARREAIYRLRYDVWVTELSHAQPLHFP
                     KGMITDELDAVSLHAAALRGPDPVAAIRLIPPTGKGLPIHRVINVRFSGRPEPTGEIS
                     HLVISKIYRRRKEDGPYGAEPYLRQSEGGSLPDQGPLAMTLRGRKGPRLILGLFRLIY
                     QASKQQGLTRWFCLADVKLFTLLTRYGMPFRQMADRPPDYPDRIPASMLISEFEDRLR
                     RLDPALFEEFTQ"
     gene            1194..>1394
                     /locus_tag="PK362_10160"
     CDS             1194..>1394
                     /locus_tag="PK362_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75232.1"
                     /translation="MLLWLAFFALFMAIAFVFEAVGRGVVSRRPKNVGLFGRNVGRSC
                     QGFTTPLAAGPDVEGISTGDGSR"
BASE COUNT          274 a          446 c          428 g          246 t
ORIGIN      
        1 gccaggaaca cggcgtcggt ggcccggaag gattcgggca gggtcggaac gaatttttcg
       61 aacacgttca accgcgtgtc cagggttttg gcttcgttca aatcgtactc gtaatacccc
      121 gaccaccgga acgtctcgcc gtcggtgatt tggagaccgg acagatcgat cccccgtcgg
      181 cgcaacagat ccaggtgctt cgcgggaagg tcccggccaa cgacccccac caaccgaaca
      241 tcggtggaga agctcgccga ataggaaaaa tagaccgccg atccgcccag ggcttcggcc
      301 cgctggccaa agggcgtctt caccgaatcc agcgccaccg atccgaccac caaaacgctc
      361 acgcccgcct cctcgaactt accgtccggc ccccgcggag ccggttcatt gagtaaattc
      421 ctcgaacaac gccggatcca accgccgaag ccggtcctcg aactccgaaa tcagcattga
      481 cgcggggatc cggtccgggt aatccggcgg acgatcggcc atttgccgga agggcattcc
      541 gtaccgggtc aacagcgtaa acaatttcac atcggccaag cagaaccacc gggtcaatcc
      601 ctgctgtttc gacgcctgat agatcaagcg gaaaagcccg aggatcaacc gggggccctt
      661 tcgcccccga agcgtcatgg ccaggggccc ctggtcgggc aaggaaccgc cctcgctttg
      721 gcgcagataa ggctcggcgc cgtacgggcc gtcttctttt cgtcgacggt aaatcttgct
      781 gatgaccaag tgggaaattt cacccgtggg ttccggccgt ccactgaaac gcacattgat
      841 cacccggtgg atcggcaaac ccttgccggt ggggggaatc aggcgaatcg cggccacggg
      901 gtcgggcccc cgcagggccg ccgcgtgaag ggacaccgcg tccaattcgt cggtgatcat
      961 ccctttggga aaatggagcg gctgggcgtg ggacaactcc gtcacccaaa cgtcgtaacg
     1021 aagacggtag attgcctccc gtcgcgcggg cgactccacg aagtggaaag aaaggtcttt
     1081 aacgtccagc aggcccatgg gtggtggtaa ggtcgccccc ggaatacgcg cggagacccc
     1141 cgttcacatt aatttccaat ggttcgacgg gcgccccggc ccggaaaccg tccgtgctgc
     1201 tttggcttgc gttcttcgcg cttttcatgg cgatcgcctt tgttttcgag gcggtcggcc
     1261 gcggcgtcgt gtcccgccga ccgaagaacg tcggcctttt tgggcgaaac gtcggtcgtt
     1321 cctgccaggg gttcaccacg cctctcgcgg cggggccgga cgtcgagggg atctccacgg
     1381 gggacgggag ccgg
//
LOCUS       DAOQLS010000637         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_78148_length_1392_cov_4.095737, whole genome shotgun sequence.
ACCESSION   DAOQLS010000637 DAOQLS010000000
VERSION     DAOQLS010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78148_length_1392_cov_4.095737"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(616..693)
                     /locus_tag="PK362_10165"
     tRNA            complement(616..693)
                     /locus_tag="PK362_10165"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
     gene            complement(790..861)
                     /locus_tag="PK362_10170"
     tRNA            complement(790..861)
                     /locus_tag="PK362_10170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(827..829),aa:Val,seq:tac)
     gene            complement(914..>1392)
                     /locus_tag="PK362_10175"
     CDS             complement(914..>1392)
                     /locus_tag="PK362_10175"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNC75233.1"
                     /translation="DEALALLPALLGKGFPDGVLAPETFEVFRVEGGVAKLGVDTPRE
                     VFPMEINLTELVSLEKECYVGQESVKRLSALGPPPRGLRSVKLSGAVPAGTPALVKGE
                     EVGRLTSVVQSPSLRAPIGLALLRREMCQPGQTLEFATRTGPATGQVVPFPNQPAE"
BASE COUNT          322 a          400 c          414 g          256 t
ORIGIN      
        1 cgtggtctgt tttggtgcgg ggaatgcggc gccatgttca ccacggaaca acagaagggc
       61 cataactatt accgctgcac ccgcaagaaa ggtacttgct atcagcccta tgtccgcgaa
      121 gaggtcctga aggaacaggt tttggctgag gtcaataagt tccttcttcc cggcgattgg
      181 gccgacaaga tgttggagga aatccagatc cgttatcggc agtcggccca atcaagggaa
      241 agttccgccc aagccctacg gggtgagatc gccgccacgg acgcccggct ggacaagctg
      301 gtggacgcct tcatatcagg cgatatggaa aaggacgatt atcagcggaa acgccgggag
      361 ttggtggcgg cgaaaatgga tttaaaaacc aaattggagg ggcttgggcg gaacagcgac
      421 tttcggctcg aacctgcaaa aaaactcatc aattggtcga aagaactcaa taaaagcgtt
      481 tttgaaggca attggcgcgc gctggttcaa agtcttgaga atctcggctc gaaccacagg
      541 attcaaaaca aaaccgcccg gctttccgtc gagaaccaat ttgcggtcct cgcagaagcc
      601 gggcggcatg taaactggcg gggtcgacgg gatttgaacc cgcggtctcc gccttgacag
      661 ggcggcgtgt taaccgagct acaccacgac cccaaaaatt cgttgcttgg gacgtgtatt
      721 atacacgaat caagcacgcc gtccaatgcc aatttcatcc aaccatccac cggcacaccc
      781 ttcaaaatct gggcggtgag ggatttgaac ccccgaccct tcgcgtgtaa ggcgaatgct
      841 ctaccgctga gctaaccgcc caaaaattga tcttcgccct tgaaaaaacg acgtttaccg
      901 aagggacgcc gcgtcactcg gcgggctgat tggggaaggg gaccacttgc cccgtggccg
      961 gtcccgtgcg ggtcgcgaat tccagggttt gtcccggttg gcacatctcc cgccgaagca
     1021 gggccaaacc gatgggggcg cggagggagg gcgattgaac aacggaggtg aggcgcccca
     1081 cctcctcccc tttgaccagg gccggcgttc cggcggggac ggcgccggac aatttgaccg
     1141 accgcaggcc ccgggggggc ggtcccaggg ccgagaggcg cttgacgctt tcctggccca
     1201 cgtaacactc cttttccaaa gagacgagtt cggttaaatt gatttccatg gggaagacct
     1261 cgcggggggt atccaccccc aatttggcca ctcccccctc gacccgaaac acctcgaagg
     1321 tttccggggc cagcacgcca tcgggaaacc ctttgccgag aagggcgggc aacagcgcca
     1381 aggcctcgtc gg
//
LOCUS       DAOQLS010000638         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_78161_length_1392_cov_3.537023, whole genome shotgun sequence.
ACCESSION   DAOQLS010000638 DAOQLS010000000
VERSION     DAOQLS010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78161_length_1392_cov_3.537023"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..738
                     /locus_tag="PK362_10180"
     CDS             106..738
                     /locus_tag="PK362_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75234.1"
                     /translation="MPRFPSRWRAFEGIEGLPLIIVASNALVFLFDLVQPGLTAFLRL
                     SHPALAAGEYWRLVTFLFVPPPMNPLFMVFWLYLVFLYANALERAWGSFRFTVYYLVG
                     SLATAAVGFFPSPEPVPNAYLNASLFLAFTALFPDMELLLFFILPVKVKVLGYLTWVW
                     LAWDFWAMGPTTRLAIAAAILSYLVLLGPALRERAMLRWQVFRNRRRWKN"
     gene            842..1045
                     /locus_tag="PK362_10185"
     CDS             842..1045
                     /locus_tag="PK362_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75235.1"
                     /translation="MAPVDGAAIRSFPPFFAAAHFLKTTAARPSVGLPSVLGAAILWR
                     GGAAHCRDFNMPVTLKTVARVGG"
     gene            1200..>1392
                     /locus_tag="PK362_10190"
     CDS             1200..>1392
                     /locus_tag="PK362_10190"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNC75236.1"
                     /translation="MKTPPPGSGPPPKTVAGLFHGFQFALTILAGLALGHWLDLRRGW
                     APYGTLGGFFGGAVVGFYQL"
BASE COUNT          177 a          443 c          441 g          331 t
ORIGIN      
        1 agtcacccag gggtccaagg gcccggccgc ggccaacgtg aagcccgaga ccaagcccgc
       61 gccggccgcc tgaacgccgc ccggcgaccc gccgggaacc gacccgtgcc gcgttttccg
      121 tcgcgctgga gggccttcga ggggatcgaa ggcctccccc tcatcattgt cgcctccaac
      181 gccctggtat tcctgtttga tctcgtccag ccggggttga ccgcgttttt gcggctctcc
      241 cacccggctc tggccgccgg ggagtattgg cggctcgtca cgttcctctt cgtaccgccc
      301 cccatgaacc ccctgttcat ggtgttttgg ctctatttgg tgtttttgta cgccaacgcc
      361 ctggagcggg cctggggctc cttccgattc accgtctatt acctggtggg ttccctcgcc
      421 acggccgccg tgggtttttt tccgtccccc gaacccgtgc ccaacgccta tttaaacgcg
      481 agcctgttcc tggccttcac ggcgctcttt ccggacatgg aattgttgct cttttttatt
      541 ttgccggtga aggtcaaggt tttggggtat ttgacctggg tgtggctggc gtgggatttt
      601 tgggcgatgg gcccgacgac gcgcctggcg atcgccgcgg cgatcctcag ttatttggtt
      661 ttgctggggc cggcgcttcg ggagcgggcg atgcttcgct ggcaggtttt tcggaaccgt
      721 cgccgatgga aaaattaagg gcgtcgccca ggtccggcgc ggccacgacg cggggaaatc
      781 gttcccaggc cgcgaaccgc tttttccgtt cggtgaaggt gtcggaggtt cggcggacgc
      841 cttggcgccg gttgatggcg cggcgatccg ctccttcccg ccgttctttg ccgccgccca
      901 ttttttgaag acgacggcgg cgcgaccgtc ggtcggtctg ccgtcggtcc tcggagcggc
      961 gatcctgtgg cgcggggggg cggcccattg ccgggatttt aacatgcccg ttacattaaa
     1021 aaccgtcgcc cgggtcgggg gataaatagt ttttcaattt cgttacgaaa ggccttccgc
     1081 gggccggttt gacaccctcg ggcccctttg ctaacattcg cccggacgtc gttggtccct
     1141 ccgcttcatc cgatcaccat cgtgggtttt tacgcggcgc gctcgcccga ggagcttcat
     1201 tgaaaacacc gccgccggga tccggtccgc ccccaaaaac cgtggccggc ctttttcacg
     1261 gctttcaatt cgccctcacg attctcgcgg ggttggctct ggggcactgg ttggaccttc
     1321 ggcggggatg ggcgccctac ggcaccttgg gcggtttttt cggcggggcc gtggtggggt
     1381 tttaccaatt gg
//
LOCUS       DAOQLS010000639         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_78243_length_1391_cov_5.168413, whole genome shotgun sequence.
ACCESSION   DAOQLS010000639 DAOQLS010000000
VERSION     DAOQLS010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78243_length_1391_cov_5.168413"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..407)
                     /locus_tag="PK362_10195"
     CDS             complement(27..407)
                     /locus_tag="PK362_10195"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNC75237.1"
                     /translation="MGIWKARKAAGVLASVFLAAGGARAEWAGLRPANQSGPSEAYRL
                     NCGAENFDFTDGGGNWWMKDEPFTALYRWGFTGGQAGGQSTADIQGTTEDAIYQTHHY
                     GPAGMAYRLEVPNGRYTVRLHFAA"
     gene            576..>1391
                     /locus_tag="PK362_10200"
     CDS             576..>1391
                     /locus_tag="PK362_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC75238.1"
                     /translation="MDSPLARPRRIKSERRRLPRRLTDRLWDVRRRYTYGVPAAILFA
                     LTLLGLYAFWLEPNRLIVKRVDIPLPGWPGGRPLRVVAIGDIHGGAPYMTAQKIKSMV
                     QLANRQKPDVVALLGDYVILGVLGGRFMEPETVAEALSGLSAPGGVFAVLGNHDWWYN
                     GPRLRTALERAGFRVLEDEVATITVDGRKVHVAGLADEWTRRPDVEKTLALLPAGEPA
                     LVLTHGPDLFPKIPRLNGLTLAAHTHGGQVRLPFMGAPIVPSRYRQRYVAGIVR"
BASE COUNT          229 a          471 c          427 g          264 t
ORIGIN      
        1 gcgggaacca aaagcggtgg gcgcagttat gccgcgaaat ggagcctgac ggtgtagcgg
       61 ccgttgggaa cttccaatcg ataggccatg cccgcgggtc cgtagtggtg ggtttggtag
      121 atggcgtctt cggtcgtgcc ttggatgtcg gccgtggatt ggccgccggc ctggccgccc
      181 gtgaaccccc atcgatagag agcggtgaag ggctcgtcct tcatccacca gtttcccccg
      241 ccgtccgtga agtcgaagtt ttccgccccg caattgagcc ggtacgcttc ggacggcccg
      301 ctttgattcg ctggccgcaa ccccgcccac tccgcccgcg ctccgcccgc cgccaaaaac
      361 accgatgcca acacccccgc ggccttccgt gccttccaaa tccccatgga tgctcctcct
      421 gtcggggccg gcgctgcgcg gccgggccga aattgcaaag tttaaatgcc cacgtgggca
      481 accgcaggta gaaagtatca cggttcctaa aacttgtcaa ggggggggtg aacttttttt
      541 gatccgggag agctataatt ccgggacacc cctttatgga ctctcccctc gcccgccctc
      601 gacgaattaa aagcgagcgg cggcgtttgc cccgccggct gaccgaccgc ctttgggacg
      661 tgcgccgccg ctacacctac ggcgtccccg ccgcgattct cttcgccctg acgcttctgg
      721 ggctctacgc cttctggctg gaacccaatc gcctcattgt taaacgggtg gacatccccc
      781 tgcccggatg gcccggcggg cgcccccttc gggtggtggc gatcggcgat attcatggcg
      841 gggcgcccta catgaccgcc cagaaaataa aatccatggt tcaattggca aaccgccaaa
      901 aacccgacgt ggtggcgctg ttgggagatt acgtgatcct gggggtcctc ggcggacgtt
      961 ttatggaacc ggaaaccgtg gccgaggcgt tgtccggcct ctccgccccg ggcggggtgt
     1021 tcgccgtgct tggcaatcac gattggtggt acaacggccc ccgccttcga acggccttgg
     1081 aacgcgccgg ttttcgagtg ctggaggacg aagtcgccac gatcaccgtg gacgggcgaa
     1141 aagtccacgt ggccggtctg gccgacgaat ggacccgccg acccgacgtt gagaaaacct
     1201 tggccctcct tccggcgggg gagcccgccc tggtgctgac ccacgggccc gacctttttc
     1261 cgaaaatccc ccgtctgaac ggcctcaccc tggccgcgca cacccacggc ggccaggtgc
     1321 gcttgccctt catgggagcg cccatcgttc cctcccgtta ccggcagcgc tacgtggccg
     1381 ggatcgtccg g
//
LOCUS       DAOQLS010000640         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_78364_length_1390_cov_3.764794, whole genome shotgun sequence.
ACCESSION   DAOQLS010000640 DAOQLS010000000
VERSION     DAOQLS010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78364_length_1390_cov_3.764794"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..596
                     /locus_tag="PK362_10205"
     CDS             123..596
                     /locus_tag="PK362_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC75239.1"
                     /translation="MSAKTDRRLKEKKIGEVVNPRLIQAPADISVQRALEVMRENKSS
                     YLVISEGKRVVGMFTESDVTRKLLNKKSEWGRPVRDFMTADPIVLRLDDPVGRAVDLM
                     AENDIYHVPLVNEKQELINVLSVRTLIRFLAEFYPGEVYNIPPNPHQVHHTAEGG"
     gene            599..>1390
                     /locus_tag="PK362_10210"
     CDS             599..>1390
                     /locus_tag="PK362_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNC75240.1"
                     /translation="MAVKEKKIVPSVTVRFAGDSGDGMQLTGDQFTDTTAVMGNDFAT
                     LPDFPAEIRAPAGTLPGVSSFQIQFADSLIFTPGDMADVLIAMNPAALKVNTRHVKKG
                     GLMILNENAFDKDGLEKAAYASNPLEDGSLRDWQVLKVPLSDLTANALKDHPLRPNEV
                     DRCKNFFALGLMFWLYDRSFDHTVDWISKKFAKKPEFVNANIAALKAGYNHADITELM
                     PTRFEVRKSALEPGTYRKITGNEATAIGLITAAELAKKILFLGSYP"
BASE COUNT          315 a          462 c          368 g          245 t
ORIGIN      
        1 ccagggtcga tttttcggaa aaaggacttc catcgacggt agtatcggct ttattgcccg
       61 cttcctccct tttatctaca atgattcttc cgcaaacgca tccagggact gcgaggcccg
      121 ccatgagcgc caaaaccgat cgtcgcttaa aagagaagaa aatcggggaa gtggtcaatc
      181 cccggctgat tcaggctccc gcggacatct ccgttcaacg ggccctggaa gtcatgcgtg
      241 aaaacaaatc ctcctacctc gtgatttccg agggcaagcg ggtggtcggc atgttcacgg
      301 aatccgacgt cacgcgcaaa ctgctcaaca agaaaagcga atggggccgt cccgtccggg
      361 acttcatgac cgccgacccc atcgtcctcc gcctggacga ccccgtgggc cgggcggtgg
      421 acctcatggc ggaaaacgac atttaccacg tccccctggt caacgaaaaa caggaattga
      481 tcaacgtcct gagcgtacgg accctgatcc gatttttggc cgaattttac ccgggggagg
      541 tgtacaacat cccccccaac ccgcaccaag tgcaccacac cgccgagggg ggctgaccgt
      601 ggccgtgaaa gaaaaaaaga tcgtcccctc cgtcaccgtc cggttcgccg gcgactcggg
      661 ggacggcatg cagctgacgg gggaccagtt caccgacacc accgccgtca tggggaacga
      721 tttcgccacc ctgccggatt tcccggcgga aatccgcgcc ccggccggca ctctgccggg
      781 ggtttccagt tttcaaattc aattcgccga ctccctgatt ttcacccccg gggacatggc
      841 cgacgtgctg atcgccatga accccgccgc cctcaaagtg aacacccgcc acgttaaaaa
      901 gggcggactg atgattttga acgaaaacgc cttcgacaag gacggcctgg aaaaggcggc
      961 gtacgcctcg aaccccctgg aggacggcag tctgcgggac tggcaggttc tcaaggttcc
     1021 cctctcggac ctcacggcca acgccctcaa ggaccacccc ctccgtccga acgaagtcga
     1081 ccgatgcaaa aactttttcg ccctggggct catgttctgg ctctacgacc ggagcttcga
     1141 ccacacggtc gactggatat ccaagaaatt cgccaaaaag cccgaattcg tgaacgccaa
     1201 catcgccgcc ctgaaagccg gctacaacca cgccgacatc acggagctca tgcccacccg
     1261 cttcgaagtt cggaaatcgg ccctggagcc gggcacctat cggaagatca cgggcaacga
     1321 ggccacggcc atcggcttga tcaccgccgc cgaattggcc aagaaaatcc tcttcctggg
     1381 gtcctacccc
//
LOCUS       DAOQLS010000641         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_78386_length_1390_cov_3.008989, whole genome shotgun sequence.
ACCESSION   DAOQLS010000641 DAOQLS010000000
VERSION     DAOQLS010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78386_length_1390_cov_3.008989"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..699)
                     /locus_tag="PK362_10215"
     CDS             complement(415..699)
                     /locus_tag="PK362_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNC75241.1"
                     /translation="MLHFEWDDSKDAQNQKKHGVPFGLAQYAFADRKRVIAEDLAHSG
                     TEKRFYCYGRVGEGILTVRFTYRKDRVRIFGAGYWRKGKKIYETENQIHL"
     gene            complement(864..1172)
                     /locus_tag="PK362_10220"
     CDS             complement(864..1172)
                     /locus_tag="PK362_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75242.1"
                     /translation="MMKLKGRLSTFVIGVIVGGGIVFGWISFQDRNRTQIWHAALSEL
                     AKNAHVQFNQRGCMDSHLHEELEKYAIMNEHGRWCGVLAREENPFVAVQDIPVCSETK
                     "
     gene            complement(1230..>1390)
                     /locus_tag="PK362_10225"
     CDS             complement(1230..>1390)
                     /locus_tag="PK362_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC75243.1"
                     /translation="EEAAKLFNGLSAGGKVEMPLGTSPWGSYFGMFRDKYGIEWMVDY
                     DTKYKGQR"
BASE COUNT          310 a          348 c          368 g          364 t
ORIGIN      
        1 tctccactta gggccgtatt gtggcccgtg tctgaccgag gcgagggagc cgaggaaatg
       61 gttggagggc gctagcccgg aaaccgcagg ccggggagtt tttgaagacg aaaggcccta
      121 cagcgtgaag ctgtagggcc tttttctttg tttacgctaa ggcaatgtgg ccggaccgga
      181 ctggaaattg attattttaa accctgccgg ggaggctatt tatacctcgc cgcgtaggca
      241 tccaataggg ttcggatcat cttttggtaa tgggtatggc gttttcgcgc ggcattttta
      301 aagaagtcga cgctggatcg gctcaaagac atcgtgactt tgacgttgtc ttccttaaat
      361 gcgagttctt ccggaggggg gagaaagtcg cgaatgaccc gcgcttccat gggtttatag
      421 gtgtatttga ttttcggttt cataaatctt tttccccttt cgccaatagc ccgcgccaaa
      481 aatgcggacc ctgtctttcc ggtaggtaaa ccgcacggtc aaaatacctt cgcccacccg
      541 gccataacaa tagaaccgtt tctctgtccc gctgtgggcc aaatcctcgg caataacacg
      601 cttcctgtcc gcgaaggcat actgggccaa gccaaagggg accccgtgtt tcttttggtt
      661 ttgagcgtcc ttggaatcgt cccactcgaa gtgaagcatt tggacctcaa agggcgttat
      721 gaattaccat ataactatac atacgattgt atggtaaatc aaggggtggg caggtcgggt
      781 tggctggggg ggcttcggcg cggcggggag ggcttggcta aagcaactat cggaatccag
      841 tccccgtaat ctcggctacg acgttatttg gtctccgagc aaacagggat gtcttgaacg
      901 gcgacaaaag gattttcttc ccgggctaaa acgccacacc atcgtccatg ctcattcata
      961 atggcgtact tctcgagttc ctcatgtaaa tgggaatcca tgcaaccacg ttgattgaat
     1021 tgaacatggg cattttttgc gagttcggaa agggccgcat gccagatttg agttcgattt
     1081 cggtcttgaa aactgatcca accaaaaaca atgccaccac cgacaattac gccgataacg
     1141 aatgtggaca gtcgcccttt caatttcatc atggttttct cactcctggc ctttgggaga
     1201 atgaaattac gatcggtgtt ccgggggtgc tacctttgcc ccttgtattt ggtgtcgtag
     1261 tccaccatcc attcaatgcc gtatttgtcc cggaacattc cgaaatagga tccccagggg
     1321 ctcgttccca ggggcatttc cactttcccg ccggccgaaa ggccgttaaa tagtttggcg
     1381 gcctcttccc
//
LOCUS       DAOQLS010000642         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_78732_length_1387_cov_3.046547, whole genome shotgun sequence.
ACCESSION   DAOQLS010000642 DAOQLS010000000
VERSION     DAOQLS010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78732_length_1387_cov_3.046547"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="PK362_10230"
     CDS             <1..179
                     /locus_tag="PK362_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNC75244.1"
                     /translation="ALGAYFAFSGLINMGMVMGLMPVTGVPLPFLSYGGSGLVGSFMA
                     VGLLLSIHLRRYLL"
     gene            208..>1387
                     /locus_tag="PK362_10235"
     CDS             208..>1387
                     /locus_tag="PK362_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNC75245.1"
                     /translation="MKREIIANVLPEETRIAVLEDGVLAEFMIERPALGMAKLVGNIY
                     KGKIENVLPGISSAFVNTGFEKNAYLYVTDVVSSSKDRHIEKMVRRGEDIMVQVAKEA
                     IGTKGMKVTMDISLPGRYLIYMPKSEHVGVSKNIEEPEERERLRRIVEGENLPGGVIV
                     RTEAEGAEEDELRREMKYLARLWEGVQRKFESSATGTLVHRELGLTFQVARDLLTDDA
                     QIFLIDDREEYRDVRGFVEMLAPELAERVQHYTGKTPIFQAFGVDRDLAHIRQQRIDL
                     PSGGYIIIQEAESLCAIDVNTGKFIGHKSQEETVTATNLEAADEVAKQLRLRNIGGII
                     VIDFIDMRRKRNQHKVVESLHHATRNDRAKIKILPITRLGLVEMTRERRRESLLSLLS
                     E"
BASE COUNT          281 a          445 c          422 g          239 t
ORIGIN      
        1 cggccctcgg cgcgtacttc gctttttcgg gtctgatcaa tatgggcatg gtgatgggct
       61 tgatgcccgt caccggcgtg cccctgcctt ttttgtccta cggcgggtcg gggctggtgg
      121 ggtcgttcat ggccgtgggg ttgctgttgt ccatacactt gcgccgctac ttattgtaga
      181 tcggccgttt ttcggaggaa aaacaaaatg aagagagaaa tcatcgccaa cgtgttgccc
      241 gaggaaaccc gcatcgccgt cctggaagac ggcgtgctgg ccgagtttat gatcgagcgc
      301 ccggccctcg gcatggccaa gctggtcggc aacatttaca aggggaaaat cgaaaacgtg
      361 ctccccggca tttccagcgc cttcgtcaac accggcttcg agaagaacgc ctatttgtac
      421 gtcaccgacg tcgtctcgtc gtccaaggac cgccacatcg agaagatggt ccgccgcggg
      481 gaggacatca tggtccaggt ggccaaggag gccatcggca ccaaggggat gaaggtgacc
      541 atggacatct cgctcccggg ccgctacctg atctacatgc ccaaaagcga gcacgtgggc
      601 gtctccaaaa acatcgagga gcccgaggag cgggaacgcc tgcgccgcat cgtggagggg
      661 gaaaacctgc ccggcggcgt catcgtccgc accgaggccg aaggcgccga ggaggacgaa
      721 ctgcggcggg aaatgaaata cctggcccgc ctttgggagg gcgtccagcg caaattcgaa
      781 tcctccgcca cgggcaccct ggttcaccgg gagctgggcc tgaccttcca ggtcgcccgg
      841 gacctgctca ccgacgacgc ccagatcttt ttgatcgacg accgggagga gtaccgggac
      901 gtccgcggct tcgtggagat gctcgcgccc gagctggccg agcgcgtcca gcactacacc
      961 ggcaagaccc ccatcttcca ggctttcggt gtggaccggg acctggccca catccgccaa
     1021 cagcgcatcg acctgccctc cggcggctac atcatcatcc aggaggccga gtccctctgc
     1081 gccatcgacg tcaacacggg caagttcatc ggccacaagt cccaggaaga gaccgtgacc
     1141 gccacgaacc tcgaggccgc cgacgaggtg gccaaacagt tgcgccttcg gaacatcggc
     1201 ggcatcatcg tcatcgactt catcgacatg cgtcggaaac gcaaccagca caaggtggtg
     1261 gagtccctcc accacgccac gcggaacgac cgggccaaaa tcaagattct gcccatcacc
     1321 cgcctgggcc tggtggaaat gacgcgggaa cgccgacggg aatccctgct ctcccttttg
     1381 tccgagc
//
LOCUS       DAOQLS010000643         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_78835_length_1386_cov_3.550714, whole genome shotgun sequence.
ACCESSION   DAOQLS010000643 DAOQLS010000000
VERSION     DAOQLS010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78835_length_1386_cov_3.550714"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PK362_10240"
     CDS             complement(<1..471)
                     /locus_tag="PK362_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC75246.1"
                     /translation="MTRAVFVSGLRVLDVSKSFPSSRGPRPVLKNISFHVPPGGLVCL
                     LGPNGSGKTTLLKTIGTLLLPDGGRVLLDEVDVHRASVRAKEKIGFASTEDQSFYGRL
                     SARMNLWFYGQMHGMPNALFERRLRELSSLLDLSGSLDRPFRELSSGQKQRYLLA"
     gene            complement(468..>1386)
                     /locus_tag="PK362_10245"
     CDS             complement(468..>1386)
                     /locus_tag="PK362_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC75247.1"
                     /translation="RVKDPGVESAASAPGSLTLPWDPVRKIIGLLSGGDQEILALADR
                     SASDHFLFSHVVNVTIVALRLGLALDLRGAELETLGLSAFLHDLGLIAHMEITAKPTK
                     LSPEETQRLRGHVREGQKLLDLFGGWTEELKATVSRVIGESHEREEGQGYPAQLSADR
                     IHLFAKVIAMADVYEAMTHMRPWRPRTVPHDVLRSFVEENAAEFDPMLIRAMIDTFSL
                     YPPGSFVRLSTGEIGRVLTTSPGLPLRPRVWVMIDEKGGRVDGQRVVNLGTKPGTFIT
                     EAVDETKIKTTDQRLALALRAQRWWVRGL"
BASE COUNT          273 a          416 c          459 g          238 t
ORIGIN      
        1 ggcgagcagg tagcgttgct tttgtccgct ggaaagctcc cgaaagggcc ggtccagact
       61 gccggacaag tccaacaacg acgacaattc ccggaggcgt ctttcgaaga gggcgttggg
      121 catcccgtgc atttggccgt aaaaccacag attcatccgg gcggacaacc gcccgtaaaa
      181 cgactgatcc tcggtcgagg cgaacccgat cttttccttg gcgcgaaccg acgcccggtg
      241 aacgtcgacc tcgtccaaaa gaacccggcc gccgtcgggc aggagcaagg tgccgatggt
      301 tttcagcagc gtggtcttgc ccgaaccgtt ggggcccaac aggcacacca accccccggg
      361 cggaacgtga aacgaaatgt ttttcaggac cggccgcggg ccccgggagg acggaaagga
      421 tttggaaacg tcgaggacgc gcagaccgga cacgaaaacg gcccgcgtca aaggccccga
      481 acccaccagc gctgggcgcg gagggcgagg gccaatcgtt gatcggtggt ttttattttt
      541 gtctcgtcga cggcttcggt gatgaacgtg ccgggcttgg tgccgaggtt cacgacgcgc
      601 tggccgtcca cgcggccgcc tttttcgtcg atcatcaccc acacccgggg gcggaggggc
      661 aacccggggc tggtcgtcaa cacccgcccg atttcccccg tggaaagacg gacgaaggac
      721 cccggcgggt aaagggaaaa ggtgtcgatc atggcgcgaa tcagcatcgg atcgaattcg
      781 gccgcgtttt cctccacaaa ggaccgcagg acgtcgtgag gcacggtgcg ggggcgccaa
      841 gggcgcatgt gggtcatggc ctcgtacacg tcggccatgg cgatgacttt ggcgaacaaa
      901 tggatgcgat cggcggacaa ttgcgccggg tagccctgcc cctcctcccg ttcgtggctt
      961 tccccgatga cgcgggacac cgtggccttc aattcctccg tccagccgcc gaaaagatcg
     1021 agaagttttt gcccttcccg gacgtgcccg cgcagacgct gggtttcctc ggggctgagt
     1081 ttggtcggtt tcgcggtgat ttccatgtgg gcgatcaagc ccaggtcgtg caggaaagcc
     1141 gacaggccca gggtttccaa ttcggcgccc cgaaggtcca gggccagacc caggcgcagg
     1201 gccacgatgg tgacattgac gacgtggctg aataggaaat ggtcggaggc ggaccggtcg
     1261 gcgagggcga ggatttcctg atcgcccccg gacaaaagac cgatgatttt tcgcacggga
     1321 tcccagggaa gggtcaaaga ccccggggcc gaagcggcgg attcgacccc ggggtctttg
     1381 accctt
//
LOCUS       DAOQLS010000644         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_78850_length_1386_cov_3.195342, whole genome shotgun sequence.
ACCESSION   DAOQLS010000644 DAOQLS010000000
VERSION     DAOQLS010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78850_length_1386_cov_3.195342"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PK362_10250"
     CDS             <1..445
                     /locus_tag="PK362_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HNC75248.1"
                     /translation="RRSRGAALFALTAVAALAPTAYWVGRDVAALDRLRGTTPHLLAA
                     AAPEWRAPSPTVLRAPEDGLRSVSFRLLAPAAQSVWVGGSFNDFNAADHPLTRTSEGV
                     WETTVRLAPGRYEYKFKVDGRWELDPANPERTPAPRECSLLEIGS"
     gene            442..>1386
                     /locus_tag="PK362_10255"
     CDS             442..>1386
                     /locus_tag="PK362_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75249.1"
                     /translation="MNRVGVPWVAAFLLAAGCLGPLPPRGRVRPVGGPTAEPNVVTPP
                     ALPPASGETTPFPSTVPAEGFPPMEIPPATGPAVGLLPVFWTPAPKSDPLAAARYMAA
                     RPGLRATALFPDSYFTDDANGRQARALFLSLISSGALEVALTLPGRPVLPLLMDTDNA
                     KSSTVPVAALPPRFAWPQDVTEQIALARAAHRRRWRSDPSVVVLPWDAVQGPELAELA
                     KFKLRWGLMASTPGSPVLIEDDRLSLVRPSLPPAGAATRRDWWKRGPGPGLSAGATWG
                     PVQVATLEELAAWESWLSTETFRWAKVSETVRESVSTSV"
BASE COUNT          163 a          505 c          463 g          255 t
ORIGIN      
        1 ccgccggtcc cggggggcgg cgctgttcgc cctgacggcc gtggcggctt tggcgccgac
       61 ggcctattgg gttggacgcg atgtggccgc cctggaccgg ctccggggaa ccacgcccca
      121 ccttctcgcg gccgcggctc ccgagtggcg cgcgccgtcg ccgactgttc tccgggcgcc
      181 cgaggacggt cttcggtccg tgtctttccg ccttttggcc ccggcggcgc aatccgtttg
      241 ggtcggcggt tccttcaacg attttaacgc cgccgatcat cccctcacgc gcacgtcgga
      301 gggagtctgg gagaccaccg tccgtctcgc cccgggccgt tacgaataca aatttaaagt
      361 cgacggccgg tgggaactgg atcccgccaa ccccgagcgc accccggcgc cccgggaatg
      421 ttcactgctg gagatcggtt cgtgaaccgg gtcggcgtcc cttgggtcgc ggccttccta
      481 ttggccgccg ggtgtttggg ccccctgcct ccccggggac gggtgcgccc cgtcggcggg
      541 ccgaccgccg aaccgaacgt cgtgacgccg ccggcgcttc cgcccgcttc gggcgaaacg
      601 actccctttc cctccacggt ccccgccgaa ggatttccgc cgatggaaat tcccccggcg
      661 accgggcccg cggtgggcct cttgcccgtg ttctggacgc cggcccccaa atccgacccc
      721 ttggcggccg ctcggtatat ggcggcgcgt cccggccttc gggcaacggc cctctttccc
      781 gattcgtatt tcaccgatga tgccaacgga cgacaggccc gggccttgtt tttgtcgctc
      841 atttcctccg gggccctgga ggtcgcgctc acgctgcccg gtcggccggt gttgcccctc
      901 ttaatggaca cggacaacgc caaatcgtcg accgtgcccg tggccgcgct gcctccccgc
      961 ttcgcctggc cccaggacgt gacggagcaa atcgccctgg cccgggcggc ccatcgccgc
     1021 cgttggcgtt ccgatccgtc cgtggtggtc ctgccctggg acgcggtgca gggccccgaa
     1081 ttggccgaat tggcgaaatt taaattgcgt tgggggctga tggcgtccac gcccggctcc
     1141 ccggtgctga tcgaagacga ccggctgtcc ctggtccgtc cgtccctgcc ccccgccggc
     1201 gccgcgaccc gacgcgattg gtggaaacgg ggtcccggtc cgggcctgtc cgccggagcg
     1261 acctggggtc ccgtccaggt ggccacgctg gaggaattgg ccgcttggga atcctggctg
     1321 tcgacggaga cctttcggtg ggccaaagtg tcggagaccg tgcgggaatc ggtctccacg
     1381 tcggtg
//
LOCUS       DAOQLS010000645         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_79497_length_1380_cov_3.938868, whole genome shotgun sequence.
ACCESSION   DAOQLS010000645 DAOQLS010000000
VERSION     DAOQLS010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79497_length_1380_cov_3.938868"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PK362_10260"
     CDS             complement(<1..759)
                     /locus_tag="PK362_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75250.1"
                     /translation="MKLRLKLLTIGILFSTPLRAEIHDPTGSGLRVTDVVGFSGQIIS
                     STTSLRSASSIGVLGSTITLHAASGLELRTGLWGGGPSLKSTALEQIIIFGSPRTARL
                     VIPPGAIARDYAVFTGTSPATEPLNASPAILQTATRNLARASSYSNPANGRLWEIYVQ
                     DDNGVKQSGALLRSATLTLPFDDADQDGYVDGSAPPVRVKSLSIWWLDEVHSLWVKVP
                     NPTINTSSQTVTALIPHFSVYAVMGAPSFDPSESF"
     gene            complement(831..>1380)
                     /locus_tag="PK362_10265"
     CDS             complement(831..>1380)
                     /locus_tag="PK362_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC75251.1"
                     /translation="INQLTAGILVMIFLVYVVLVVLFESLVEPFIILSAVPLCLIGVA
                     GGLVFFGLPLSTGVLVGVIMLAGVVVNNAIMLLDHLNNYTDRDEPLADRLRKSVLARM
                     RPIFLTAGSAVLGFLPMMLDTSESGVLWRPLAVTLVFGLIFSTVLTLYVTPALTHFLL
                     NDIPQWLTGRRFLAKTAPNPTP"
BASE COUNT          333 a          336 c          472 g          239 t
ORIGIN      
        1 gaaggattcc gaggggtcaa aggacggcgc ccccatgacg gcgtagacgg aaaagtgggg
       61 gatgagcgcc gtgacggtct ggctggaggt attgatggtc gggttcggaa ctttgaccca
      121 cagggagtga acctcgtcca accaccagat ggagagggat tttacgcgca ccgggggcgc
      181 gctgccgtcc acgtagccgt cctgatccgc gtcgtcaaag ggaagggtga gcgtggcgga
      241 gcggaggagt gcgccggatt gtttaacgcc gttgtcgtcc tgaacgtaga tttcccacaa
      301 ccggccgttg gcgggattgc tgtaggaact ggcccgggcc aggttgcgcg tggccgtttg
      361 gagaatggcg ggcgaggcgt tcaggggctc cgtggcggga ctggtgcccg tgaatacggc
      421 gtagtcccgg gcgatggccc cggggggaat aacaaggcgc gcggtgcggg gcgaaccaaa
      481 aatgatgatt tgttcaaggg ccgtggattt aagcgacggt ccgccgcccc aaagaccggt
      541 tcgcagttcg agccccgagg ccgcgtgaag ggtgatggtg gagcccagga cgccgatgct
      601 ggaggcggag cgaaggctcg tggtgctcga aatgatttga ccggagaatc ccaccacgtc
      661 ggtgacccgc aaaccggagc cggtgggatc gtgaatttcg gcccggaggg gggtcgaaaa
      721 taaaataccg atggtgagta gtttgagacg gagtttcacg gtagcgcaga gtatatcacg
      781 gaggggttaa aaacgtgatg aacgaaaagt aaatcagttg taacaaacgg ctaaggggtg
      841 gggttggggg ctgttttggc tagaaaccgc cgcccggtca gccattgggg gatgtcgttt
      901 aataggaagt gggttaaggc cggggtgacg taaagtgtca aaacagttga gaaaatcaat
      961 ccaaaaacga gtgtgacggc caacggacgc caaaggacgc cgctttcgga ggtgtcgagc
     1021 atcatgggta gaaaacccaa tacggcgctt cccgcggtca agaagattgg ccgcatgcgg
     1081 gccaaaaccg acttccgtag ccggtcggcc aggggttcgt cccggtcggt gtagttgttg
     1141 agatggtcta aaagcataat ggcgttatta accaccactc cggccagcat gatgaccccc
     1201 accagcactc cggtgctcaa tggcaggccg aaaaagacca acccgcccgc cacgccgatc
     1261 aagcagaggg gaaccgcgct aagaatgata aagggttcca ccagggactc aaaaagaacc
     1321 accaaaacca catagaccag aaagatcatg accaaaattc cggcggtcag ctgattgatg
//
LOCUS       DAOQLS010000646         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_79725_length_1378_cov_4.920635, whole genome shotgun sequence.
ACCESSION   DAOQLS010000646 DAOQLS010000000
VERSION     DAOQLS010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79725_length_1378_cov_4.920635"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1060
                     /gene="galT"
                     /locus_tag="PK362_10270"
     CDS             44..1060
                     /gene="galT"
                     /locus_tag="PK362_10270"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNC75252.1"
                     /translation="MPELRRDPVIGRWVIISTERSRRPGGFSTQRETQGSVPCPFCAG
                     NEEKTPPEILAFAPKNRAANAPGWWVRVIPNKYPALQIEGDLHRTGEDLFDKMDGLGA
                     HEVIVETEDHNRQLEDLDPSRVKDVLWAYRDRILDLKKDPRFEYILVFKNRGSAAGAS
                     LAHAHSQLIATPMVPIRVKQELRGAEEYFEQKGRCIFCQILQAEVEKGERVIEANEDF
                     VALAPFASRFPFEMWILPKVHSSDFESMSEAQFASLSVLLKSVLGRLNAVLDFPPYNF
                     VVHTSPLKTKGLAHYHWHIELMPKIVHTAGFEWGAGFYINPTPPEQAARYLREAPNGT
                     STAG"
     gene            1057..>1378
                     /locus_tag="PK362_10275"
     CDS             1057..>1378
                     /locus_tag="PK362_10275"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNC75253.1"
                     /translation="MKILFAASEAVPFCKTGGLGDVVGALAEALKVRGHDVRLVLPHY
                     CPPGSDEEPPGSGPLSLTIPVDGRDHPVRVWETRFGPDVPAYLIDSPSHFAREGLYRD
                     SRGVD"
BASE COUNT          250 a          464 c          418 g          246 t
ORIGIN      
        1 ccctcggtgg aaaccgccgc ggcgccggcc ccggaggccc cccatgcctg agcttcgacg
       61 cgacccggtc atcggccggt gggtcatcat ttcgacggaa cgcagccgcc gcccgggggg
      121 gttcagcacc cagcgcgaaa cccaggggtc cgttccctgc cccttttgcg cgggcaacga
      181 ggaaaagacg ccgcccgaga ttctggcctt cgccccgaaa aaccgggcgg ccaacgcccc
      241 gggctggtgg gtgcgcgtca ttcccaataa ataccccgcc ctgcagatcg agggcgacct
      301 ccaccgtacc ggcgaggatc tgttcgacaa gatggacggg ttgggcgccc acgaagtcat
      361 tgtggaaacc gaggatcaca accggcaatt ggaggatttg gaccccagcc gggtgaagga
      421 cgttctttgg gcctaccggg accgcattct ggatttgaaa aaagacccgc gcttcgaata
      481 catcctcgtt ttcaaaaacc ggggctcggc ggcgggggcg tccctggccc acgcccattc
      541 ccaactcatc gcgacgccga tggtgccgat tcgggtgaag caggaactgc gcggcgccga
      601 ggaatatttc gagcagaagg ggcgttgcat tttctgccag attctccagg ccgaagtgga
      661 gaaaggcgag cgcgtgatcg aggccaacga ggatttcgtg gctttggcgc ccttcgccag
      721 ccggttcccc ttcgaaatgt ggatcctacc gaaggtgcac agctcggatt tcgaatccat
      781 gtccgaggcc cagttcgcct ccctgagcgt ccttttgaaa agcgtgctcg ggcggctgaa
      841 cgccgtgttg gattttccgc cctacaattt cgtcgtgcac acctcgcccc tgaaaacaaa
      901 ggggttggcc cattaccatt ggcacatcga gctgatgccc aaaatcgtgc acaccgccgg
      961 ttttgaatgg ggcgccggct tctacatcaa tcccaccccg ccggaacaag cggcgcgcta
     1021 cctccgggaa gccccgaacg gaacgtcgac cgcgggatga aaatcctctt cgcggccagc
     1081 gaagcggtcc ccttttgtaa aaccggcggt ttgggcgacg tggtcggcgc cctggccgaa
     1141 gccctcaagg tccggggcca cgacgttcgg ctggtcctcc cccattattg ccctcccggc
     1201 agcgacgagg agccgcccgg ctccggcccc ctttccttga cgattcccgt cgacgggcgc
     1261 gaccacccgg tccgcgtgtg ggaaacccgc ttcggccccg acgtgcccgc ctatttgatc
     1321 gacagcccgt cccatttcgc ccgggaaggc ctctaccgcg attcccgggg cgtcgatt
//
LOCUS       DAOQLS010000647         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_79905_length_1377_cov_2.487141, whole genome shotgun sequence.
ACCESSION   DAOQLS010000647 DAOQLS010000000
VERSION     DAOQLS010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79905_length_1377_cov_2.487141"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PK362_10280"
     CDS             <1..735
                     /locus_tag="PK362_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNC75254.1"
                     /translation="KPMIVYLSTGVGLRAAVWGVAWSSSTLESGILNGVGPGVVFNHY
                     GHYLAGYLNGDTNTLKFITDASRDLSISGTVLDFGGSPIPGVAMTLSGGISATALSVS
                     ASTGGYSADHLFEGSYTVTPSKSGYAFLPVSLSYNPLQSSMAGQNFQGDLVDFSTVGN
                     LFNPVGGEQVTFNYSIIPGHVSLKVYSLRGAPVKTLVDQDQSAGNYSVTWDGRDIDGH
                     VVASGIYLVYFEANQNKSTKKVAVVK"
     gene            861..>1377
                     /locus_tag="PK362_10285"
     CDS             861..>1377
                     /locus_tag="PK362_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HNC75255.1"
                     /translation="MIMGILISTVWALDPRTGGTTLLETVDARAAALGETGSALTGDL
                     SGVNFNPAVLTTLANTQLETQFEVAPGDVRTGLVSFGQTGSRAGWGASLVALDAGTID
                     ITPTSGAPYSKRAQQDLVGTLSGGVLLGSWFRVGATMKGLQSKLAGTYSGTAVAGDGG
                     ILVDLPVEGLRL"
BASE COUNT          253 a          364 c          439 g          321 t
ORIGIN      
        1 aaacccatga tcgtttatct gtccacgggc gttggtttgc gcgcggcggt gtggggggtg
       61 gcgtggtcct cttcgacctt ggaatcgggg attttgaacg gggtcggccc gggtgtcgtt
      121 ttcaatcact acggccatta cctggcgggc tatttgaacg gcgacacgaa cacgttgaaa
      181 ttcatcacgg acgcgtctcg ggatctgtca atttccggga cagtcttgga ctttggggga
      241 tctccgattc ccggcgttgc aatgaccctg tcggggggga tttcagccac cgcgctctcg
      301 gtgtcggcct cgaccggggg gtattccgcc gaccatctgt tcgaggggtc ctacacggtc
      361 acgccgtcaa aatcagggta cgcgttcttg cccgtgtctc tctcttacaa tcctctccaa
      421 tcgtccatgg cggggcagaa ttttcaaggg gatttggttg atttttcgac cgtgggaaat
      481 ttgttcaacc cggttggagg ggaacaggtg acgttcaact actctatcat ccccgggcat
      541 gtgtcgctga aagtgtacag tttgcggggt gctccggtga aaaccctggt tgaccaggat
      601 caatccgcgg gaaattattc tgttacctgg gacggtcgtg acatcgacgg ccacgtggtg
      661 gccagcggca tctatctggt ctatttcgaa gcgaaccaaa acaaaagcac caagaaagtc
      721 gcggtggtga aatgacaaat cggattgttt tgttgctcgt catcccggcg aaagccggga
      781 tccagtctgt aaaacggtgt ttaaggttca atattaaaca ctggatcccg gctttcgccg
      841 ggatgacagc aaaatcggtt ttgatcatgg ggatcttgat ttcaacggtc tgggccctcg
      901 acccgcgcac cggcggcacg acgctgttgg aaaccgtcga tgcccgggcc gcggccctgg
      961 gggaaaccgg gtccgccttg accggggatt taagcggcgt caatttcaat ccggcggttc
     1021 tgacgacctt ggcgaatact cagctggaga cccaattcga agtggcgccg ggcgatgtgc
     1081 gcactggact cgtctcgttt ggccaaacgg gctcccgggc ggggtggggc gcatcgctcg
     1141 tcgccttgga cgccgggacc atcgacatta cccccacgtc gggcgcgccc tattccaaac
     1201 gggcccagca ggatttggtg ggcacgcttt cgggaggcgt tctgttgggc tcctggtttc
     1261 gcgtgggggc gacgatgaag gggctccaat ccaaattggc cgggacctat tcgggaaccg
     1321 ccgtggcggg agacggcgga attcttgtgg atcttcccgt ggagggattg cgattgg
//
LOCUS       DAOQLS010000648         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_80224_length_1374_cov_3.072024, whole genome shotgun sequence.
ACCESSION   DAOQLS010000648 DAOQLS010000000
VERSION     DAOQLS010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80224_length_1374_cov_3.072024"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PK362_10290"
     CDS             <1..604
                     /locus_tag="PK362_10290"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNC75256.1"
                     /translation="ALARGIAELPRGDETLRGELADLAAREGRPALHARLAAVDPEAA
                     AAIPPNNLQRVIRALEIYRLTGRPITHWHRAAGGRSPWPFRWFGLKWPPADYDDHLKR
                     RCARLWPGLLEETRRLLAGNLSPAAPAFESLGYREAVRCLTGVLSPDDGSELFYLQTR
                     HYAKRQMTWFRGEKTLEWVPVDLPPDPQKTAAEILARLKR"
     gene            652..>1374
                     /gene="hflX"
                     /locus_tag="PK362_10295"
     CDS             652..>1374
                     /gene="hflX"
                     /locus_tag="PK362_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNC75257.1"
                     /translation="MERALLVGLRLKGKNNATADSSLNELKGLLETAGGVAEDVWIQE
                     RVSRDPATLIGKGKVEEIARAVAEKGWGAVVINDDLSPTQQQNLQEHIPAKIIDRTRL
                     ILDIFAQRARTREGKLQVELAQMNYLLPRVTEKFGRFEQQVGGIGTRGPGERKLEISQ
                     RRIRDRIVRLRREIEEVRKQRALQRENRQRLPMPLVALVGYTNAGKSTLLNALRENPA
                     GAVYADDKLFATLDPTTRRARLP"
BASE COUNT          266 a          438 c          439 g          231 t
ORIGIN      
        1 cgcgttggct cggggcatcg ccgagttgcc gcggggggac gagaccctgc gcggcgaatt
       61 ggccgatctg gcggcgcgcg aagggcgccc ggcccttcac gcgcgtttgg ccgccgtgga
      121 cccggaggcg gcggccgcca ttcccccgaa caatttgcag cgggtcattc gcgccctgga
      181 aatttaccgt ttgacgggcc gccccatcac ccattggcac cgggccgccg ggggccggtc
      241 cccctggccc ttccgttggt tcggattaaa atggcccccg gcggattacg acgaccattt
      301 aaaacggcgt tgtgcccggt tgtggccggg cctgctcgag gaaacccggc gccttttggc
      361 cgggaatctt tcgccggcgg cgcccgcctt tgaatccctg ggataccggg aggcggtccg
      421 ttgtttgacg ggcgtcttga gcccggacga cggttcggaa ttgttttatc tccaaacccg
      481 ccactacgcc aaacgtcaga tgacctggtt tcggggggag aaaaccctgg aatgggtgcc
      541 ggtggacctt cccccggatc cccaaaaaac ggcggcggag attctcgccc gattgaagcg
      601 ctaaggcttt gccttccccc cggcctccgg aaccgctaaa ataagcgtcc catggaacgc
      661 gcgctcctcg tcggactccg cctcaagggc aaaaacaacg ccaccgccga ctcctcctta
      721 aacgaattga aggggttgtt ggaaacggcg ggcggggtgg ccgaagacgt gtggattcaa
      781 gaacgcgttt cccgggaccc ggcgaccctg atcggcaagg gcaaagtcga ggaaatcgcc
      841 cgggcggtgg ccgaaaaagg ctggggggcc gtcgtgatca acgacgacct ctcgccgacc
      901 caacagcaaa acctgcagga acacatcccg gccaagatca tcgaccgtac ccggttgatt
      961 ttggatattt tcgcccagcg ggcccgaacg cgggaaggga agttgcaagt cgagttggcc
     1021 cagatgaatt accttcttcc ccgggtcacg gaaaagttcg gccggtttga acagcaagtc
     1081 ggcggcatcg gaacccgggg acccggcgaa cgaaaactcg aaatcagcca gcggcggatc
     1141 cgggaccgca tcgtccggct tcgccgggaa atcgaagagg ttcgaaagca acgggccctt
     1201 cagcgggaaa accgccagcg attgcccatg ccgctggtgg ccctggtggg ttacacgaac
     1261 gccgggaaat caacgctttt gaacgccctc cgggaaaacc ccgccggagc ggtttacgcc
     1321 gacgacaagc ttttcgccac gctggatccg accacccggc gggcccgttt gccg
//
LOCUS       DAOQLS010000649         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_80515_length_1371_cov_4.309271, whole genome shotgun sequence.
ACCESSION   DAOQLS010000649 DAOQLS010000000
VERSION     DAOQLS010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80515_length_1371_cov_4.309271"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PK362_10300"
     CDS             <1..1027
                     /locus_tag="PK362_10300"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNC75258.1"
                     /translation="HRRLTVTRPLLAITLGDPAGIGPLVGARAALDPALRRRSRPLLV
                     GDAWILHRLLHRRGIAVKPLPSIDDYLDRPGVANVLHVPHPEIRTLRRGAAQRVGGES
                     AALSIRAAVALALAGRVAAVVTGPASKESFRLAGLPHPGHTEMLAALCGIDHVEMVMA
                     AGRRRTVLITRHLPLKDVSAALTTKKIIEAVLRVDAWGRTHLGLRKRPRWILCGLNPH
                     AGDGGLLGREEIRTVAPAAAALRKKGLPLLGPWPADVAWARHAAGESDFVASLYHDQG
                     MIPLKTLDGRRLVNVTVGLPFVRTSPGHGTAFDLAAGPRAEAMADAEPTLEAARVALD
                     LALRNPR"
     gene            1024..>1371
                     /locus_tag="PK362_10305"
     CDS             1024..>1371
                     /locus_tag="PK362_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75259.1"
                     /translation="MKKTLRAILLALLATAAARAEDRSPISVGLFGLTAVPLSPSEIT
                     DGYEMGSAYGARLRHEVNDRWTLGGQFANHAFRSKADGQRSVTLQSVVFVAQRRFFDS
                     RTWMPFANATLGIS"
BASE COUNT          199 a          512 c          431 g          229 t
ORIGIN      
        1 ccaccgccga ttgaccgtga cccggccgct tttggccatc acgctcggcg accccgcggg
       61 catcggcccg ctcgtgggag cccgggcggc cctggatccc gcccttcggc gccggtcccg
      121 gcccctcctg gtgggggacg cctggatcct gcaccgactt ctccaccgac gcggcatcgc
      181 cgtaaaaccc ctcccctcca tcgacgacta tcttgaccgg cccggcgtgg ctaacgtgtt
      241 gcatgtccct catccggaga tccgcaccct tcgccggggc gcggcccaac gggtgggcgg
      301 ggaatccgcc gcgttgtcga ttcgggccgc ggtggccctg gccctggccg gccgcgtggc
      361 cgccgtggtc accggcccgg cctccaagga atcctttcgg ctggcgggat tgccccaccc
      421 ggggcacacc gagatgttgg cggccctgtg cgggatcgat cacgtggaaa tggtcatggc
      481 cgcgggccgt cgacggacgg tgctcatcac ccggcatctt cctttgaaag acgtatccgc
      541 ggcgttgacg acgaagaaaa tcatcgaggc ggtccttcgg gtggacgcct ggggacggac
      601 ccatctcggc cttcgaaaac ggccccggtg gattctctgc ggtttgaacc cccacgccgg
      661 cgacggcggt ctcttgggcc gggaggaaat tcggacggtg gcgcccgccg ccgccgccct
      721 tcgaaaaaaa ggcctccccc tcctcggccc ctggccggcg gacgtggcct gggcccggca
      781 cgcggccggc gaaagcgact ttgtggcaag cctctaccac gatcagggca tgattccttt
      841 aaaaacactg gacgggcggc ggttggtcaa cgtcaccgtg gggcttcctt tcgtccgcac
      901 gtcccccggc cacggcaccg ctttcgacct ggcggccggt ccccgggccg aggcaatggc
      961 cgacgccgag ccgactttgg aagccgcccg ggtcgccctg gacctggccc tgcgaaaccc
     1021 ccgatgaaaa aaaccctccg cgcgatcctt ctggcccttt tggccaccgc cgcggcccgg
     1081 gccgaggacc gctctcccat ttccgtgggg ctattcggcc tgaccgccgt tccgctttcc
     1141 cccagcgaga tcaccgacgg ctacgaaatg ggctccgctt acggcgcccg cctgcgacac
     1201 gaagtgaacg accgctggac cctgggcggg cagttcgcca accacgcctt tcgatcgaag
     1261 gccgacgggc aacggtccgt gacccttcaa tccgtcgtgt tcgtcgccca acggagattt
     1321 ttcgactccc gcacctggat gcccttcgcc aacgccacgc tcggcatcag c
//
LOCUS       DAOQLS010000650         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_80523_length_1371_cov_3.535714, whole genome shotgun sequence.
ACCESSION   DAOQLS010000650 DAOQLS010000000
VERSION     DAOQLS010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80523_length_1371_cov_3.535714"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PK362_10310"
     CDS             <1..646
                     /locus_tag="PK362_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC75260.1"
                     /translation="LINCAGAHADRVARLFGLAADHVLVPFKGLYWKIRPERNGLVKE
                     SLYPVPDLAFPFLGVHLTRGAAGDVYAGPTAVPALGRENYGIFEGLRPGEMGQSLWRL
                     ARLYAGRDRSFRALVHRELLHYTRRGFLAAARRLVPALREDDLVPAHKVGIRPQLVRR
                     SDDRLEMDFHVEKNERSLHVLNAISPAFTCAFSFAAHLLDSWEKKESTDALAVS"
     gene            633..>1371
                     /locus_tag="PK362_10315"
     CDS             633..>1371
                     /locus_tag="PK362_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC75261.1"
                     /translation="MRSLKEKLQDRQARVGVMGLGYVGLPLAIAFAEAGLRVFGFDVD
                     EEKCRALRAGRSYVGDVPARDVQKAIGAGRLRVSSRPADLKGLDAVVICVPTPLRKTK
                     DPDISYIVAAAEVLAPRVSPGQLIVLESTTYPGTTREVILPKLQERGLVAGRDFFLAF
                     SPERIDPGNPRFGLRNTPKIVSGLTPRCTALAEALYGTVTETLVPVSSLETAEMAKLL
                     ENTFRAVNIALVNEVAQMCERLKLSATS"
BASE COUNT          217 a          444 c          456 g          254 t
ORIGIN      
        1 gctgatcaat tgcgcgggcg cccacgccga ccgggtggcg cgcctgttcg ggttggcggc
       61 cgaccacgtg ctggttccct ttaaagggct gtattggaaa attcggccgg agcgaaacgg
      121 actggtgaag gaaagcctct atcccgtccc ggatttggcg tttcccttcc tgggcgttca
      181 tttgacccgc ggcgcggcgg gggacgtgta tgcgggcccc accgccgtcc cggccctcgg
      241 gcgggaaaat tacgggattt tcgagggtct gcgcccgggg gaaatgggcc agtccctctg
      301 gcgcctggcg cggctctacg ccgggcggga ccgttccttt cgggcgctgg tccatcggga
      361 actgctccac tacacgcgcc gggggttcct ggcggccgcc cgacggttgg ttcccgccct
      421 gcgggaagac gatttggtcc ccgcccacaa agtcgggata cggccccaat tggtccggcg
      481 gtccgacgac cgcctcgaga tggatttcca cgtggagaaa aacgagcgtt ccctgcacgt
      541 gctgaacgcc atctccccgg ccttcacctg cgcgttttcg ttcgcggccc atttgctgga
      601 ttcgtgggaa aaaaaggagt cgacagatgc ccttgcggtc tcttaaggaa aaacttcagg
      661 accggcaggc ccgggtgggc gtcatgggcc tggggtatgt cggattgccg ctcgccatcg
      721 ccttcgcgga ggcgggcctg cgggtgtttg gtttcgacgt ggacgaggaa aaatgccgcg
      781 ccctgcgggc ggggcggtcc tacgtggggg acgtgccggc ccgcgatgtt caaaaggcca
      841 tcggggcggg ccgcctgcgg gtctcctcgc ggcccgcgga tttgaaaggg ctggacgccg
      901 tcgtgatttg cgttcccacc cccctgcgca aaaccaaaga cccggacatc agctacatcg
      961 tcgccgccgc cgaggtcctc gcgccccggg tgtctcccgg gcagctgatc gtgctggaaa
     1021 gcacgaccta ccccgggacg acccgggaag tcatccttcc caaattgcag gagcggggcc
     1081 tggtggccgg ccgggatttc ttcctcgcct tttcgcccga gcggatcgac cccgggaatc
     1141 cccgtttcgg cctgcggaac acgcccaaaa tcgtttcggg cctgaccccc cggtgcaccg
     1201 ccctggccga ggccttgtac ggcacggtga ccgaaaccct ggttccggtg tcctccctgg
     1261 aaacggccga aatggcgaag ctgttggaaa acacgttccg cgccgtgaac atcgccctcg
     1321 tcaacgaagt ggcgcaaatg tgcgaacggt tgaaactgag cgccacttcg t
//
LOCUS       DAOQLS010000651         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_80835_length_1368_cov_5.368621, whole genome shotgun sequence.
ACCESSION   DAOQLS010000651 DAOQLS010000000
VERSION     DAOQLS010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80835_length_1368_cov_5.368621"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1368)
                     /locus_tag="PK362_10320"
     CDS             complement(<1..>1368)
                     /locus_tag="PK362_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNC75262.1"
                     /translation="YWFPCHDSPQDRVTTEMLVRVPRGFTAVSNGRLVKKQARGTQTL
                     FHWKQDIPHATYLVTLAVGQFAEIKDRWRRVPVNYYVTPGREDDARRAFGKTPKMMEF
                     FSSKIGVPYAYPKYAQVAALDFIYGGMENTSATTQTALTLHDERAHLDFSSDPLVAHE
                     LAHQWFGDLLTCRDWAHAWLNESFATYFEALFTEFDKGAEEFAQEIRQNGEIYFDEDA
                     EHYRRPIVTKVYKQPSDLFDRHLYEKGSRVLHMIRYLLGDRDFWKSIHTYVADNRETV
                     VETRDLLDAIEKSTGRNLSRFFDQWVYGGGHPEYKVRSWWDPKTREAVVRVVQAQNTN
                     GETGLFALPIEFAFWVKGKEKRFKETVEKKTHLFRFKLSGEPELTLFDPDHWILKKVD
                     FPKSEAAWLRQLTMDPHVLGRSDAAKALGKLGGPAAVDALGKALLRETFWGVKADIAQ
                     ALGQI"
BASE COUNT          239 a          401 c          444 g          284 t
ORIGIN      
        1 ggatctggcc cagggcctgg gcgatgtcgg ctttaacgcc ccagaaggtt tcccgtagaa
       61 gggccttccc caaggcgtcg acggcggcgg gaccgccgag cttgcccagg gctttcgccg
      121 cgtcggaccg ccccagcacg tggggatcca tcgtcaattg ccgcaaccag gcggcctcgg
      181 acttggggaa atccactttc tttaaaatcc agtggtccgg gtcgaagagg gtcagttccg
      241 gctccccgga caatttgaag cggaacaggt gggttttctt ttccaccgtt tccttgaacc
      301 gcttttcctt gcccttgacc cagaaggcga attcgatggg cagggcaaag aggccggttt
      361 cgccgttggt gttctgggcc tggaccacgc ggacgacggc ttcccgggtt ttcgggtccc
      421 accaggaccg caccttgtat tccgggtggc cgccgccgta aacccactgg tcgaagaacc
      481 gcgaaaggtt ccgtcccgtg gatttttcaa tggcgtccag caaatcccgg gtctcgacga
      541 ccgtttcccg gttgtccgcc acgtaggtgt ggatggattt ccagaaatcc cggtccccca
      601 acaagtagcg gatcatgtga aggacgcggg agcccttctc gtacaaatga cggtcgaaga
      661 ggtccgacgg ctgcttgtac accttggtca cgatggggcg gcggtaatgc tcggcgtcct
      721 cgtcgaaata gatttcgccg ttttggcgga tttcctgggc gaactcctcg gcgcctttgt
      781 cgaactccgt gaagagcgcc tcgaagtacg tggcgaagct ctcgttcagc caagcgtggg
      841 cccaatcccg gcaggtgagg agatcgccga accactggtg ggccagttcg tgggccacga
      901 gcgggtcgct ggaaaaatcc aaatgggccc gctcgtcgtg gagggtcagg gccgtctggg
      961 tggtggcgct ggtgttttcc atgccgccgt agatgaagtc cagggcggcc acctgggcgt
     1021 atttggggta ggcgtagggc acgccgattt tggaggaaaa gaactccatc attttgggcg
     1081 tcttgccgaa ggcccgtcgg gcgtcgtcct cgcggccggg ggtcacgtaa taattcacgg
     1141 gcacccggcg ccaccgatct ttgatctcgg cgaattggcc cacggccagg gtcaccaggt
     1201 aggtcgcgtg cggaatgtcc tgtttccagt ggaacaacgt ttgggtgccc cgggcctgtt
     1261 ttttaaccaa tcgaccgttg gacaccgccg tgaagccccg gggaacccgc accagcattt
     1321 ccgtcgtcac ccggtcctgg ggggaatcgt ggcaggggaa ccagtagc
//
LOCUS       DAOQLS010000652         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_80996_length_1367_cov_3.901677, whole genome shotgun sequence.
ACCESSION   DAOQLS010000652 DAOQLS010000000
VERSION     DAOQLS010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80996_length_1367_cov_3.901677"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1367
                     /locus_tag="PK362_10325"
     CDS             <1..>1367
                     /locus_tag="PK362_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75263.1"
                     /translation="GTHAAAWAGAGPLVFLLALTGVTVSLLGGGGVLGLYLGHVAYNL
                     FHPGAPLAVEPVPVQFYLTREVREPLEKDPAGLQNALRRLAALLHGYARDIRVGMGPE
                     PSIQWERDGSSLSLRVTPADLGSPAAFERRGQLLADRLKTHLAGPLRGRIAGFPVIHA
                     RASDFPGGTLSRFLRENPGSKITLFQELGGAASDEHLNEIFSRHTADFPDRLFDLRPG
                     AAPLSLGAFVADPARSSWWASLFATNQRIVRENLRAMDEALASGHWPRVEAQLGGNDF
                     SRALTVWLFENYDRARPVREPIEPDVEFMIWQMMWIQQRPWVEGPWAIRGLSPRAAFE
                     TALERLYQSELLLVKTSVLRNARMAEILRRMPVNPTVQGVLIFGAAHYYGLQNDLISW
                     IAPDRDNRPGEGTGAREIAWMARLPSYRLHRDLVRLLEGEFVHPTAEDRRPFSPRVQR
                     LADLR"
BASE COUNT          204 a          460 c          433 g          270 t
ORIGIN      
        1 tcggaaccca cgccgcggcc tgggcgggcg cggggcccct ggttttcctg ttggccttga
       61 cgggcgtcac ggtgtcgctc ctgggggggg gcggcgttct gggcctatat ctgggccacg
      121 tcgcctacaa tcttttccac ccgggcgccc ccctggcggt cgaacccgtg ccggtccaat
      181 tttatttgac ccgggaggtt cgggaaccgt tggagaagga ccccgccggg cttcaaaacg
      241 ccttacgacg tctcgccgcc ctccttcacg gttacgcccg ggacattcgg gtgggaatgg
      301 ggccggagcc ttccattcag tgggagcgcg acgggagttc cctgagtttg cgggtgacgc
      361 ccgcggacct cggttcgccc gcggcctttg aacggcgggg ccagctgttg gccgatcggc
      421 tgaagaccca tttggcgggc cccctgcggg ggcggatcgc ggggttcccg gtcatccacg
      481 cccgggcttc cgatttcccc gggggcaccc tctctcgttt tcttcgggaa aaccccggga
      541 gcaaaatcac cctgtttcaa gaactcgggg gggcggcctc cgatgaacac ctaaacgaaa
      601 ttttttcgcg ccacaccgcg gattttcccg accgcctgtt cgaccttcgc cccggcgccg
      661 cccccctgtc cctgggggcc ttcgtggcgg accccgcccg gtcgagttgg tgggcctccc
      721 tcttcgccac caatcagcgg atcgtccggg agaaccttcg ggccatggac gaagcgctgg
      781 cctcgggcca ctggccccgg gtcgaagcgc agttgggcgg caacgacttt tcccgggcgt
      841 tgaccgtctg gctgtttgaa aattacgacc gcgcccgccc cgtacgggaa cccatagaac
      901 cggacgtgga gttcatgatt tggcagatga tgtggattca gcaacggccc tgggtcgaag
      961 ggccctgggc cattcgcggt ctttctcccc gggccgcctt tgaaacggcc ctggaacgcc
     1021 tttaccaaag cgaattgctg ttggtcaaaa cctccgtttt gcgcaacgcc cggatggccg
     1081 aaattctccg gcggatgccc gtcaacccca ccgtccaagg ggttctgata tttggcgcgg
     1141 cccattatta cggactgcaa aacgatctca tctcttggat cgcccccgac cgggacaatc
     1201 ggccggggga aggcaccggc gcccgggaga tcgcctggat ggcccgtttg ccctcctatc
     1261 gcctccaccg ggacctggtc cgcctgcttg aaggggaatt cgtccatccc acggcggaag
     1321 atcgaaggcc tttttcgccc cgggtccaac ggttggcgga cctccgt
//
LOCUS       DAOQLS010000653         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_81565_length_1362_cov_3.688600, whole genome shotgun sequence.
ACCESSION   DAOQLS010000653 DAOQLS010000000
VERSION     DAOQLS010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81565_length_1362_cov_3.688600"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /gene="infB"
                     /locus_tag="PK362_10330"
     CDS             complement(<1..1330)
                     /gene="infB"
                     /locus_tag="PK362_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNC75264.1"
                     /translation="MKTDKGLVTFLDTPGHEAFTAMRARGAQATDLVILVVAADDGVM
                     PQTVEALNHARAAGVPIVVAINKCDLPTANPQRIKQELANLNLAPEDWGGKTVMVEVS
                     AKAKKNLDKLLEMILLEAELLELKANPNRPAQGIVVEAKLDPRRGPVATVLIQKGTLR
                     VGDAFVCGVTAGKIRALTNDKGERVKDAPPAYPVEILGLSGTPQAGDKLVVLPSDREA
                     REIAERRQGIATAEARRTRQHLTLEAFHEAAAAGKVKQLPIVLKADVQGSLEAIRDSL
                     EKLNGAEISVRVIHAGIGGITNSDVVLADASDAVILGFNVRPDPSSESLAQREGVEIK
                     TYRIIYDMVNDVKAGLEGLLDPEEKEVTTGWADVRKTFTAPKVGFVAGCMVTDGKVTR
                     TGRARLVRNGAIVFEGAIGSLRRFKDDVREVEKGYECGISLANFQDVKVGD"
BASE COUNT          204 a          431 c          444 g          283 t
ORIGIN      
        1 tgtcccccac cttgacgtcc tgaaagttcg ccagggagat gccgcactcg taccccttct
       61 ccacttcccg aacgtcgtct ttgaaccggc gtaggctgcc gatggcgccc tcgaagacga
      121 tggcgccgtt ccgcaccagg cgggcacgcc ccgtgcgggt gacttttccg tcggtgacca
      181 tgcagcccgc cacgaagccc actttggggg ccgtgaaggt tttgcgaaca tcggcccatc
      241 cggtcgtgac ttccttttcc tcgggatcca gcaggccttc caagccggcc ttgacgtcgt
      301 tgaccatgtc gtaaatgatt cggtaggttt taatttcgac gccttcccgt tgggccaggg
      361 attcggagga ggggtcgggg cggacgttga agccgaggat gacggcgtcg gaggcgtcgg
      421 ccaacaccac gtcggaattg gtgatcccgc cgatgccggc gtggatcacg cggaccgaaa
      481 tttccgcccc gttcaatttt tccagggagt cgcggatggc ttccagggag ccctgcacgt
      541 ccgccttcaa gacgatgggc agctgcttca ccttgcccgc ggcggcggct tcgtggaagg
      601 cctcgagcgt caaatgctgg cgcgttcgac gcgcctcggc ggtggcgatg ccctggcggc
      661 gttcggcgat ttcccgggct tcgcggtcgc tgggcagcac gaccagcttg tcgccggcct
      721 ggggcgtgcc cgaaagcccc aaaatttcaa cgggataggc cgggggcgcg tccttcaccc
      781 gttcgccctt gtcgttggtc aacgcgcgga ttttgccggc ggtgacgccg cagacgaagg
      841 catcgcccac ccgcaacgtg cctttttgaa tgagcacggt ggccacgggg ccccggcggg
      901 ggtccagttt ggcttcgacg acgatgccct gggccgggcg gttggggttg gccttcaatt
      961 ccaacaattc ggcttccagg agaatcatct ccagcaattt gtccaaattt ttcttcgcct
     1021 tggccgacac ctccaccatg accgtcttgc cgccccaatc ttcgggcgcc aggttcaggt
     1081 tcgccagctc ttgcttgatg cgctgggggt tcgcggtcgg caggtcgcat ttgttgatgg
     1141 cgaccacgat gggcaccccg gcggcgcgcg cgtggttcaa ggcttcgacg gtttggggca
     1201 tgaccccgtc gtcggcggcc accaccaaaa tgaccaagtc ggtggcctgg gcgccccggg
     1261 cgcgcatggc cgtaaaggct tcgtggcccg gcgtgtcgag gaaggtgacg aggcccttat
     1321 cggttttcac ttgataggcg ccgatgtgct gggtgattcc cc
//
LOCUS       DAOQLS010000654         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_81671_length_1361_cov_4.400459, whole genome shotgun sequence.
ACCESSION   DAOQLS010000654 DAOQLS010000000
VERSION     DAOQLS010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81671_length_1361_cov_4.400459"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK362_10335"
     CDS             complement(<1..272)
                     /locus_tag="PK362_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC75265.1"
                     /translation="MTALLEIRDLRVEYRRGNVAAVRGVSIDLAPGESLGLAGESGSG
                     KSTLALSVLRLLAPEAVRAVTGRILFKGRDVLTLADRELRSLRGGAV"
     gene            complement(269..1066)
                     /locus_tag="PK362_10340"
     CDS             complement(269..1066)
                     /locus_tag="PK362_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC75266.1"
                     /translation="METLGAALVGGLVLAAILAPWAAPADPLRQDLTHRLEAPSARHW
                     MGTDDLGRDVVSRILYGARVSLTVGALAVGLSVLLGTLVGLAGGYFGGWVDAVLMRLV
                     DVLLCFPSIFLILMVIAFLEPNIANIMAIIGLTSWMGLARLVRAETLSVRDRDFVWAA
                     RGLGLSTPRLLTVHILPHVLSPVLVAATLGVGGAVMTESALSFLGLGVQPPSPSWGNM
                     LSAGKDYIHLAWWLTLFPGLAIFLTVLGFNLLGEGLRDRLDPRLSGQ"
     gene            complement(1105..>1361)
                     /locus_tag="PK362_10345"
     CDS             complement(1105..>1361)
                     /locus_tag="PK362_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC75267.1"
                     /translation="LRNALIPVVTILGLSLPDLIGGSFIFETIFAYPGMGRLGFDAVM
                     ARDYPVVMAVGTIAALLTLLGNFAADLTYAAVDPRIRFGR"
BASE COUNT          310 a          494 c          404 g          153 t
ORIGIN      
        1 acggccccgc cccgaaggga acgaagttcc cggtcggcca aggtcagcac gtcccgcccc
       61 ttaaagagga ttcgtccggt gacggcccgc accgcttccg gcgccagcag acgaaggacc
      121 gaaagcgcca gggtcgactt gccggacccc gactcccccg ccaacccgag ggattccccc
      181 ggcgccaggt cgatcgaaac gccccgcacc gcggccacgt tcccccgacg gtattccacc
      241 cgcaggtccc gaatttccaa aagggccgtc attggccgga cagccgcggg tccagacggt
      301 cccgcaaccc ctcgcccaac aggttgaaac ccaacaccgt caggaaaatc gccaagccgg
      361 gaaaaagcgt cagccaccag gccaggtgga tgtagtcctt gcccgccgaa agcatgttgc
      421 cccaggaggg cgacgggggc tgaacgccca ggcccaggaa ggaaagggcc gactccgtca
      481 tgacggcccc gcccacgccc agggtcgcgg ccaccagcac cggcgaaagc acgtggggta
      541 aaatatggac ggtcaagagc cgcggcgtcg acaaccccaa tccccgggcg gcccaaacga
      601 agtcccgatc ccggacgctc agagtttccg cccgaaccag gcgcgccagg cccatccagg
      661 aggtcaggcc gatgatggcc atgatgttgg cgatgttggg ctccaaaaag gcgatcacca
      721 tcaaaatcaa aaagatgctc ggaaaacaaa gaagcacgtc cacgagccgc atcaaaaccg
      781 cgtccaccca cccgccgaaa tagccccccg ccaatcccac cagggtcccg agcaaaacgg
      841 acagccccac ggccagggcc ccgacggtga gagacacgcg agctccgtaa agaatccggg
      901 acaccacgtc ccgccccagg tcgtcggtgc ccatccaatg gcgggccgag ggcgcctcca
      961 accggtgggt caaatcctgc cgcagggggt ccgccggcgc cgcccagggc gccaggatcg
     1021 ccgccagtac gagcccgccc acgagcgccg ctcccagggt ttccatgggg cgcccggccc
     1081 cccgccccct tcgtcgaaaa aagttcatcg gccgaaccgg atgcgggggt ccaccgccgc
     1141 gtaggtcaaa tcggccgcga aatttcccaa aagggtcaga agcgccgcga tggtccccac
     1201 ggccatgacc accggatagt cccgggccat gacggcgtcg aagcccagcc gtcccatgcc
     1261 ggggtaggcg aaaatggttt caaaaatgaa actgccgccg atcaaatccg gaagagaaag
     1321 ccccaaaatc gtcaccacgg ggatcagcgc gtttcgaagg g
//
LOCUS       DAOQLS010000655         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_81750_length_1361_cov_2.132466, whole genome shotgun sequence.
ACCESSION   DAOQLS010000655 DAOQLS010000000
VERSION     DAOQLS010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81750_length_1361_cov_2.132466"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          270 a          431 c          405 g          255 t
ORIGIN      
        1 ggttccactt tgcgccttgt ccctttcccc agaggggaga gggaggacag ggagagggtt
       61 ccttggaggg gtgtgcgcgc gattttggaa ttgaaaaagc cctggccctt cgccgaggca
      121 tcaaacggat tcactattcc aaaacgtcgg tcatcgtcga attcgtttgt gaccccgcct
      181 tcgacggaaa aacagcgtcc gagcccgtgt cctccgcccc cgcgctcaga gtggccccga
      241 acgacagtcc ccttccgacc cccgaaacaa aagagcccac ccccggacag gaagtgggcc
      301 cttctgtgtc taccgccaac aggaaatggt gtagttaccc cgatcggata caagtccatt
      361 caatcaaatt tgtgaacact tttcacgatt atcggctggc catgggcctg ccgatgtgaa
      421 cccgtgcgaa ggcctgtgac cgtttagtcg atggggtgcc cggacaaagg caacgtgcct
      481 tgaatggcac gttgggcact ttggccaagg tcgggcacgg gcgcgagatc gtgagcgcac
      541 tgggttggac cgcctccaat gcccttgtgc gggccaacat ctccacgcaa ctggagtgca
      601 tcctcgcccc cattgggggg gaggaccgag gagagggggt ccgccctgtc gggccaaaat
      661 ttgtgcccgc tatgcgggcg ccacagcgcg cctctgttct cttcgcattc ccacacagca
      721 cagcacggga cagacagaga gagagactct ctgcccttcg ggcctcgctg atcggcgggc
      781 cgcatcggat cgcttcgcca gttaacggaa gaactgaagt cgccacggaa aaaaagatcc
      841 agccggttcg agccaggaaa gaagatgggg actgccgctg aatcgctcgt ccgggaaaga
      901 aaggaatcgc tccgttcgta gcggagaaaa accgtcgcgg gccgagccgc cccaaggcga
      961 tggccagcgc agtcgtttga cgttgacggt tggtccatct tcctgggaag aaaatggaga
     1021 ccgctccgac ctttggagaa aaaccgctcg ggtggctccg gtggattcgt tcccgccgga
     1081 gcgattccgc ccatcacttc ggtgggagaa gggaaacccg ctgacggtta ttccgtcgat
     1141 cgcgggagat atgtttcccg ctccgttttg cggacaatcc tcgctccgga cgaacgcccg
     1201 tcgcggcgcc tggtttccgg actagcgtcc tccaaccatt tcctcgcctc ccacggctcg
     1261 gtcatcatac gcgccgcaac tcctgcctcc gattccgacg tttccagctc cggtcccacg
     1321 gagaagccgc gcggccagag gccgcgcccc gcttccgccc c
//
LOCUS       DAOQLS010000656         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_81780_length_1360_cov_4.846743, whole genome shotgun sequence.
ACCESSION   DAOQLS010000656 DAOQLS010000000
VERSION     DAOQLS010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81780_length_1360_cov_4.846743"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /gene="mnmA"
                     /locus_tag="PK362_10350"
     CDS             complement(<1..840)
                     /gene="mnmA"
                     /locus_tag="PK362_10350"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNC75268.1"
                     /translation="MTAPRNIVVAMSGGVDSSVAAALLHEAGHRVIGVTLRLLGKETG
                     FGCCGTTKDIDDARAVCGRLGVPHYVFDFAQTFEQKIMDPFLQSYLGGETPNPCINCN
                     RYVKFDALLKKAVTLGADAVATGHYARVEKAAGDAGPVYRLRRARDAQKDQSYVLYHL
                     GQAELSRLLFPVGDMEKADVRAAAHRLGLRTADKPESMEICFVPGANTAAYVRDEAER
                     KELLARTVRPGPIKDKTGRVLGTHKGVAFYTRGQREGLGISVGRPLYVVDLDPATNTV
                     FVGD"
     gene            complement(840..1217)
                     /locus_tag="PK362_10355"
     CDS             complement(840..1217)
                     /locus_tag="PK362_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNC75269.1"
                     /translation="MYSAKVMDHFQNPRNVGEMPEADALGKAGSPTCGDILRLYLKFT
                     GGRVAKATFKTFGCGAAIATSSVLTEIVLGKTPEELRAITNLQVAEALGGLPPIKMHC
                     SVLAEEALRSALADWAQKNRSAA"
BASE COUNT          247 a          436 c          439 g          238 t
ORIGIN      
        1 gtcgccaacg aataccgtgt tggtggcggg gtccaaatcc acaacgtaaa gggggcggcc
       61 gacggaaata cccagcccct cgcgctggcc gcgggtgtag aacgcgacgc ctttgtgggt
      121 gcccagcacc cggccggttt tgtccttgat ggggccgggc cggacggtac gggccagaag
      181 ctccttgcgc tcggcctcgt cgcgaacgta ggcggccgtg ttggcgccgg gaacgaaaca
      241 aatctccatg ctctcgggct tgtcggcggt ccgaaggccc aatcgatggg ccgccgcccg
      301 cacgtcggcc ttttccatgt cgcccacggg gaacaaaaga cgggacaact ccgcctggcc
      361 caaatgatag aggacgtaag actggtcctt ttgggcgtcc cgggcccgac ggagtcgata
      421 aacgggaccc gcgtccccgg cggctttttc cacccgggcg taatggcccg tggccacggc
      481 gtcggcgccc agggtcacgg cctttttcaa cagcgcgtcg aatttgacgt accgattgca
      541 gttgatgcag gggttcggcg tttccccccc caaataagac tggagaaagg gatccatgat
      601 tttttgttcg aaggtctggg cgaaatcgaa gacgtaatgg ggcacgccca gacggccgca
      661 gacggcccgg gcgtcgtcga tgtccttggt ggttccgcaa cagccgaacc ccgtctcctt
      721 tccgaggagc cggagggtca cgccgatcac ccggtggccc gcctcgtgca ggagggccgc
      781 cgccacggag gaatccacgc ccccggacat ggccaccacg atgttgcgcg gggccgtcat
      841 taagccgcgc tccgattttt ctgggcccaa tcggccaggg cggaacgcag ggcttcctcg
      901 gccaggaccg aacaatgcat tttgatgggg ggcagaccgc ccagggcctc ggccacctgg
      961 aggttcgtga tggcccggag ctcctccggg gttttcccga gaacgatctc cgtcaacacc
     1021 gaggaggtgg cgatggcggc gccgcaacca aaggttttaa acgtggcctt ggccacgcgg
     1081 cccccggtga atttcaaata cagacgcagg atgtccccgc aggtggggct tccggccttc
     1141 cccaaagcgt cggcctccgg catttcgccg acgttccggg ggttttgaaa atggtccatc
     1201 actttcgcgg aatacacggg atctcccgac gcttattgat tggcggcccg acggccgcgc
     1261 tcttcccaca agggcgacat ttggcgcaaa cggtccacgg ccacgggcaa aacccgcagc
     1321 gcaaaatcca cgtcggcctc ggtgttgaaa tgccccaggg
//
LOCUS       DAOQLS010000657         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_82004_length_1359_cov_1.791411, whole genome shotgun sequence.
ACCESSION   DAOQLS010000657 DAOQLS010000000
VERSION     DAOQLS010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82004_length_1359_cov_1.791411"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PK362_10360"
     CDS             <1..938
                     /locus_tag="PK362_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC75270.1"
                     /translation="VIRVIPDDSVQFLELRSQSIDFMGLTPDQYIAYDSFFQNHQKFR
                     YPSFSYTFFGFNLQRDLFKDLRVRQALALALDKREIIDGVLLGLGRSATGPFPPSSWA
                     FDPTVPELPFDPARAKALLAEAGWRDTDGDGILDKEGKPFSFTVITNQGNKMREQTAL
                     ILQAHLARIGVKMEVRILEWSSFIHDFVDKGNFDAIILGWNLGRDPDQYLIWHSSQRG
                     EGRYNFIGYQNPQADKLWEEGRRTFDQGKRQVIYRKLHRLLAQDMPYIFLYYPDALPT
                     IHKRFQNVIAAPAGIGWNFREWFVPKALQRYRLAP"
     gene            1053..>1359
                     /locus_tag="PK362_10365"
     CDS             1053..>1359
                     /locus_tag="PK362_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC75271.1"
                     /translation="MGSFLLRRLSLMLVVLVGITLMTSVVMRLAPGGLTGGVTDLSAR
                     VSAHEQRKKFMALYDLDKPWYVQYGRWLKRLSRLDFGRSFKDDRPVLEKIGERLPNTL
                     "
BASE COUNT          280 a          388 c          373 g          318 t
ORIGIN      
        1 tggtcattcg cgtgattccc gacgattcag ttcagttttt ggagcttcgg agccagtcca
       61 ttgatttcat ggggctcact cccgatcaat acatcgccta cgattcattt ttccaaaacc
      121 atcaaaagtt tcgctacccg tcgttcagct acaccttttt tggtttcaac ctccagcggg
      181 acctgtttaa agacctccgc gtgcgccagg ccctggccct ggcgttggac aaacgcgaaa
      241 tcatcgacgg tgtgctcttg ggtcttggcc gttccgcgac gggcccattc ccgccctctt
      301 cgtgggcctt cgatcccacg gtgcccgagt tgccttttga tccagcgagg gcgaaagccc
      361 tcctggcgga ggcggggtgg cgggacacgg acggagatgg aattttggat aaggagggga
      421 aacccttttc ttttacggtc atcaccaacc aaggcaacaa gatgcgggaa cagaccgcgc
      481 tcattttgca ggcccacctg gcccggatcg gggtcaaaat ggaagttcga attttggaat
      541 ggtcgtcctt cattcacgac ttcgtggaca aagggaactt cgacgccatt atcttggggt
      601 ggaatctcgg gcgggatccc gatcaatatt taatttggca ttcgagccag cggggggaag
      661 gccgctataa ttttatcggc tatcaaaacc cccaggcgga caaattgtgg gaagaagggc
      721 gccggacctt cgaccaaggc aaacggcaag tcatttaccg gaaactccac cgcctattgg
      781 cccaggacat gccttacatt ttcctttatt atccggacgc tctgcccacg atccacaaac
      841 ggtttcaaaa cgtgatcgcg gcccccgccg gcatcggctg gaacttccga gaatggttcg
      901 tgccgaaagc acttcaacgt taccgtttgg ccccctaacc atgtccgacc aaacatacga
      961 agtgtttgtt tggagcgcgg gcgtcccgcc cgcatttttt ggattgaaag ccctgtgcgg
     1021 gcgggacgcc cgcgctccaa aggcataaag gcatgggatc ttttcttctt cgtcgactgt
     1081 ctttgatgtt ggttgtgctc gtgggcatca cgctcatgac ctcggtggtc atgcggctgg
     1141 ccccgggggg actgaccggg ggcgtgacgg acttaagcgc ccgggtctcc gcccacgaac
     1201 agcgcaaaaa gtttatggcc ctctacgatt tggacaaacc ctggtatgtt caatatgggc
     1261 gctggctcaa acggctctcc cgactggact tcggccgttc cttcaaagac gaccggcccg
     1321 tcttggaaaa aatcggcgag cggttgccga atacccttc
//
LOCUS       DAOQLS010000658         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_82375_length_1355_cov_4.768462, whole genome shotgun sequence.
ACCESSION   DAOQLS010000658 DAOQLS010000000
VERSION     DAOQLS010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82375_length_1355_cov_4.768462"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PK362_10370"
     CDS             complement(<1..732)
                     /locus_tag="PK362_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75272.1"
                     /translation="MIRRPLRRHFPRAALGILLFPGLAFGFSLTDRPAGESAFHWTPY
                     FSAELYGGQSTFSGDSQSPVSGPNGSLLFVPGFRLGPRLTILPSFSVNYRQTRDVQEL
                     IGGGFLTQKQSDQSAAVKALWALTDRWKFKGYGSFKQELVQESTDEKWGQGLFDYQKT
                     AAGLELERSGMTVRSFRVGVDLYWVAFPNFDSLSADAQQFGGEVNAGTHVLDYRSLDG
                     TLSTDVLLSPRTAVSATLAASDRRFG"
     gene            complement(729..>1355)
                     /locus_tag="PK362_10375"
     CDS             complement(729..>1355)
                     /locus_tag="PK362_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75273.1"
                     /translation="VVFAHWFVEAKAEGQEPVLLSHWVPTSAFQLGNYEATKIRDEND
                     LASDGAQDLPWGYKDFVGVGAGGVVGDDRLIDGFSAAQRTQYFTNATINTKYNISEGF
                     VRTARLFLDGAPAPFTPEQFQAARETVVTLNYVNPLAATNPDLTQTSFRLTDDGRRLN
                     QFDIAQGNLKNDINFQSVFNSNLFQGRKIDLVTSPLTFRPPDERRQDQ"
BASE COUNT          273 a          372 c          449 g          261 t
ORIGIN      
        1 accgaaccgg cggtccgagg cggccagagt ggccgacacc gccgttcggg gcgaaagaag
       61 aacatcggtc gacagggtgc cgtccaaact ccggtagtcg agcacgtggg tcccggcgtt
      121 gacctcgccc ccgaattgct gggcgtccgc ggacaggctg tcgaaattcg gaaaagccac
      181 ccaataaagg tccacgccca cccgaaagct tcgcaccgtc atgcccgaac gctcgagttc
      241 cagtccggcg gccgtttttt gatagtcgaa caacccctgc ccccattttt cgtcggtgga
      301 ttcctggacc agttcctgtt tgaaggaacc gtatccttta aatttccacc ggtcggtcag
      361 agcccacagg gctttgacgg ccgcggattg gtcggattgt ttttgggtca gaaacccccc
      421 cccgatgagt tcctggacgt cccgggtttg gcggtaattg accgaaaagc tggggaggat
      481 tgtgagccgg ggccccagtc ggaatccggg gacgaacaac agggaaccgt tggggccgct
      541 gaccggggac tggctgtcgc ccgaaaaagt ggattgaccg ccgtagagtt ccgccgagaa
      601 atacggggtc cagtggaagg cggactcgcc ggcggggcga tcggtcaagg aaaacccgaa
      661 ggccaggccg ggaaagagta ggattcccag ggcggcgcgc ggaaaatggc ggcggagggg
      721 ccgacgaatc attgatcctg gcggcgttcg tcgggtggac ggaacgtcaa ggggctcgtc
      781 acgagatcga ttttccttcc ctggaagagg ttggaattaa agacggactg gaaattaatg
      841 tcgttcttta aattgccctg ggcgatgtcg aactggttca aacgccgtcc gtcgtcggtc
      901 aaccggaagg aggtctgcgt gaggtccgga ttggtggccg ccaggggatt gacgtagttc
      961 agggtcacga ccgtttcccg ggcggcctgg aattgctccg gcgtgaaggg ggccggcgcg
     1021 ccgtccagga aaaggcgggc ggtacgaaca aatccttcgc tgatgttgta cttggtgttg
     1081 atggtggcgt tggtgaagta ttgagtgcgc tgggcggccg aaaagccgtc aatgagccgg
     1141 tcgtcgccga cgaccccgcc ggcaccgacc cccacaaaat ctttatatcc ccaggggagg
     1201 tcctgggccc catcgctggc caggtcgttt tcgtcccgga ttttcgtggc ttcgtaattg
     1261 cccaattgaa aggcgctggt ggggacccaa tgggaaagga gaaccggttc ttgaccctcg
     1321 gctttggctt ccacaaacca atgggcgaat accac
//
LOCUS       DAOQLS010000659         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_82503_length_1354_cov_4.376443, whole genome shotgun sequence.
ACCESSION   DAOQLS010000659 DAOQLS010000000
VERSION     DAOQLS010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82503_length_1354_cov_4.376443"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PK362_10380"
     CDS             complement(<1..949)
                     /locus_tag="PK362_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNC75274.1"
                     /translation="MTVHPNRLQRQWILVRRWLRSSGRDVVDSTRESVGSLEAKTGAL
                     LFAAFWVAGLLGRGAPLSSAADAEIVQWARRSSPGWVVAWQAQSLVLHVLVGLSWGVV
                     FRNAWDLVGDLWKPFRRGHWTLLRQVAVTALGVFVFHAAVWLRDVGRHPALYLHLAEG
                     RWAIARGAYLLMADGRPIWLRWPLQGVAFLGLAAAGLNVVRRVGLWFWGFNRPTRLAI
                     AVLGSFLALSAVGFRAVWRAQLTPSAGPNAVVVVVDGLRSDRLFRAEGGSPAPRLVRW
                     MARGRSFTRCVPPAADADLSLMTFLTGQTPLRHGFRSRWA"
     gene            917..>1354
                     /locus_tag="PK362_10385"
     CDS             917..>1354
                     /locus_tag="PK362_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75275.1"
                     /translation="MALKPVRVDRHGAATGADEIDGGSTDVPTGAAGAAGTGFGAAGI
                     GRGVPTAETVEGTTADGAETGAAGVRGGTETGAVDPETVPGEVGGPATARGGSGTGTV
                     EPAIAPGEVVGAATARGGPLSTGETDGRSKVETAGTGAASGTGG"
BASE COUNT          261 a          463 c          506 g          124 t
ORIGIN      
        1 acgcccaacg ggaacggaac ccgtgccgaa gcggcgtttg gcccgtcaag aacgtcatca
       61 gcgacaggtc cgcgtcggcc gccgggggca cgcaacgggt gaagcttcgt ccccgggcca
      121 tccagcgcac gagccggggc gcggggcttc cgccctccgc ccggaaaagc cggtcggagc
      181 gaagtccgtc gacgacgaca acaacggcgt tgggtccggc gctcggggtc agttgggccc
      241 tccacaccgc gcgaaaaccg acggcggaaa gcgccaggaa agatcccagg accgcgatgg
      301 cgaggcgcgt ggggcgattg aacccccaaa accacaatcc gacccggcgg acgacgttca
      361 ggcccgccgc ggccaaaccc aaaaaggcga ccccttggag aggccatcgc agccaaatgg
      421 ggcggccgtc ggccatcaac aggtaagcgc cccgggcgat ggcccagcgc ccttcggcca
      481 ggtgaagata gagcgccgga tgccgcccaa cgtcccgcaa ccaaacggcg gcgtgaaaga
      541 caaagacccc cagggcggtc acggcgacct ggcgtaacaa tgtccaatgg ccacgccgga
      601 agggcttcca caggtccccg accaaatccc aggcgttccg gaacaccacc ccccaggaca
      661 acccgacgag gacgtggagc accaaggact gggcttgcca ggccacgacc catccggggg
      721 acgatcgacg ggcccattga acgatttccg cgtccgccgc cgaggaaagc ggggcgcccc
      781 gccccaggag ccccgcgacc caaaacgcgg cgaaaaggag cgcccccgtt ttggcttcca
      841 gggagcccac ggattcccgg gtggagtcca ccacgtcccg gccgctcgag cggagccagc
      901 gccggacgag aatccattgg cgttgaagcc ggttcgggtg gaccgtcacg gggcggccac
      961 cggcgccgac gagatcgatg gcggctcgac cgatgttccg accggcgcgg cgggcgccgc
     1021 cgggaccggc ttcggcgcgg ccgggatcgg aaggggggtt ccgacggcgg agaccgtcga
     1081 ggggaccacg gccgacggcg cggaaacggg cgcggcgggc gtccgcgggg gaacggaaac
     1141 gggagccgtg gacccggaaa cggtccccgg cgaggtcggg ggaccggcga cggcccgcgg
     1201 cgggagcgga acgggaacgg tggaaccggc gatcgctccc ggcgaagtcg tcggggcggc
     1261 gacggcccgg ggcggcccgc tttcgacggg cgagaccgat ggtcgctcga aggtcgaaac
     1321 ggcgggaacg ggcgcggcga gcggaacggg cggg
//
LOCUS       DAOQLS010000660         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_82519_length_1354_cov_3.685142, whole genome shotgun sequence.
ACCESSION   DAOQLS010000660 DAOQLS010000000
VERSION     DAOQLS010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82519_length_1354_cov_3.685142"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PK362_10390"
     CDS             <1..164
                     /locus_tag="PK362_10390"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175355.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC75276.1"
                     /translation="AEGVSKAILFNLCGHGHFDMGAYMEYFAGRLTDQSYEESELAMA
                     LAGLPSVKA"
     gene            184..269
                     /locus_tag="PK362_10395"
     tRNA            184..269
                     /locus_tag="PK362_10395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:Leu,seq:taa)
     gene            245..>1354
                     /locus_tag="PK362_10400"
     CDS             245..>1354
                     /locus_tag="PK362_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75277.1"
                     /translation="MGSIPTPGIYFETPRRFFPMPLKILTGSFSALEKAFVDRLRASP
                     PGLTRRTTVVTTSHQMADRLQRLLTLESGGAFANLHFQTLHTLSLETLRRSGAPVPAV
                     VNDELFHDRLLEKILLEEGRLPPERVRPLAGAHRAAVRDLMEAGVEGAAFREHFADLE
                     IPGNAKLQRLLDLADRVRETLGKLNVASSAELARRAARILEDSPDLLDPAGELIYYGF
                     YDLNGAQSEFFDAVCRAASVTLYFPCRSGRPVWAFAQRFLDLKLGAGGAVVTPLPPTV
                     DDALAGVADGLFDPAVKPEGPVGRFRIIGVSGERDDLWRAAKEIRRLKESHPDARWSD
                     FGVVARSVESRSEAARDVFESEAIPLSISDGGPLLG"
BASE COUNT          214 a          458 c          430 g          252 t
ORIGIN      
        1 aggccgaagg ggtgtccaag gccattctct tcaacctctg cggccacggg cacttcgaca
       61 tgggcgccta catggaatat ttcgcgggcc gtttgaccga tcagagctac gaggagtccg
      121 aattggccat ggcgctggcc ggccttccgt cggtcaaagc ctgagggcgc cgcggtcgtt
      181 tttgcccggg tggcggaact ggcagacgca caggacttaa aatcctgagg gccgcaaggc
      241 ccgtgtgggt tcgattccca ccccgggcat ttattttgaa acgccgagac gcttctttcc
      301 catgcccctt aaaatcctaa ccggctcctt ttccgccctc gaaaaggcct ttgtcgaccg
      361 cctgcgggcg agcccgcccg gcctgacgcg ccggacgacg gtcgtcacga cgtcccacca
      421 aatggccgac cgtctccaac ggcttttaac gttggagagc ggcggcgcct tcgccaacct
      481 ccactttcaa accctgcaca ccctgtccct ggagacgctt cgtcgatcgg gggcgccggt
      541 ccccgccgtg gtcaacgacg agttgtttca cgaccgcctc ctcgaaaaaa tcctgctgga
      601 ggaggggcgc ctgccccccg aacgcgtccg ccccctggcc ggggcccacc gggccgccgt
      661 ccgtgatttg atggaggccg gggtggaggg cgcggccttt cgggaacatt tcgccgattt
      721 ggaaattccc ggcaacgcga aattgcagcg cctgctggat ctggccgacc gggtgcggga
      781 aaccctgggg aaactgaacg tggcttcctc ggcggaattg gcccgtcggg cggcccggat
      841 tctggaagat tccccggacc ttctcgaccc cgccggggaa ctgatttact acgggttcta
      901 cgacttaaac ggggcccagt cggaattttt cgacgccgtg tgccgggccg cgtcggtcac
      961 cctctacttt ccctgccggt ccgggcgacc ggtgtgggcc ttcgcccagc ggtttttgga
     1021 cctcaagctg ggcgccggag gcgccgtcgt cacgccccta ccgcccaccg tggacgacgc
     1081 cctggccggg gtcgccgatg gactcttcga tcccgcggtg aaaccggaag gtcccgtcgg
     1141 ccggtttcga atcatcgggg tatccgggga gcgcgacgat ctttggcgcg cggccaagga
     1201 aatccgccgt ttgaaagaat cccatcccga cgcccggtgg agcgatttcg gcgtcgtcgc
     1261 ccgcagcgtg gaaagccggt cggaagcggc ccgggacgtc ttcgagtccg aggccatccc
     1321 cctgtcgatc tccgacggcg ggcccctgct cggc
//
LOCUS       DAOQLS010000661         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_82642_length_1353_cov_3.021572, whole genome shotgun sequence.
ACCESSION   DAOQLS010000661 DAOQLS010000000
VERSION     DAOQLS010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82642_length_1353_cov_3.021572"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PK362_10405"
     CDS             <1..329
                     /locus_tag="PK362_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75278.1"
                     /translation="VAEAIDRFEQTKADSLASGFLCTYTEYGTNALPRQKVPGFFYDD
                     GNLYVMRGDGLARGDRYGVRRERFVLDREERIDIDEEFDFWMAEQILRGGRGGPWVRP
                     SPPGAR"
     gene            340..>1353
                     /locus_tag="PK362_10410"
     CDS             340..>1353
                     /locus_tag="PK362_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75279.1"
                     /translation="MNLATTALRAYWPADPPLLLLGGWCVDVGPGGVPPGARVLPCPW
                     NDRARYYRAIEEADRTIERLLPPAAEALNRLHGERRTTDYWRTIAGPWLIQYVHALSD
                     RYTLLRDALREEPNLTTWVPDRRPPPPPRDTLEHLDWLGDDRYNLSLLACLSTRLGIS
                     TVIRPAPSEGAPPFPSASHGGPGFKPPWVRWSARAVDFLLGGRRRGLLAELNVSQWDT
                     WKLVLKTGGRMLPWSLGGESRLPASPDAGRRETIRLDCKPTTELESLALDLFKDCLPE
                     SLVENYQRTVALVERRLPSPVRAIVSGNGWHCHESFKAMAARERERGARLISVQHGGG
                     YGVH"
BASE COUNT          207 a          449 c          466 g          231 t
ORIGIN      
        1 gggtggccga ggcgatcgat cgatttgaac aaacgaaggc ggactccctg gccagcgggt
       61 ttctctgcac gtacacggaa tacgggacga acgcgctccc ccgccagaaa gtccccggct
      121 ttttttacga cgacggaaac ctgtacgtga tgcggggcga tgggctggcc cggggcgacc
      181 gttacggcgt ccggcgcgag cggttcgtcc tggaccgcga agagcggatc gacatcgacg
      241 aagaattcga tttttggatg gccgagcaaa tcctccgcgg cggccggggc gggccctggg
      301 tgcggccgtc cccgccgggc gcccgttaac caccgcgccg tgaacctcgc caccaccgcg
      361 ctccgcgcct attggcccgc cgatccgccc ctgttgctgc tcggggggtg gtgcgtggac
      421 gtggggccgg gcggggttcc gccgggcgcc cgcgtgctcc cctgcccctg gaacgatcgg
      481 gcgcgctatt accgggccat tgaggaagcg gaccggacga tcgagcggct gctgccgccc
      541 gccgccgaag ccctcaaccg gctgcacggg gaacgccgga cgaccgacta ttggcggacg
      601 atcgccggcc cgtggttgat ccaatacgtc cacgcgttgt ccgatcgata cacgctcctg
      661 cgggacgccc tgcgggagga gcccaactta accacctggg tcccggaccg acggcctccc
      721 ccgccgccgc gggacacctt ggagcacctc gattggttgg gggacgatcg ctacaacctt
      781 tccctgttgg cttgtttgtc cacccgcctc ggcatctcga cggtgattcg gcccgcgccg
      841 tccgaggggg ccccgccttt tccgtcggcg agccacgggg ggcccggttt taaaccccct
      901 tgggtccgct ggtccgcccg agcggtcgat tttttactgg ggggccgacg gcgcggtctc
      961 ttggccgaat tgaacgtgtc gcaatgggac acgtggaaat tggtcctgaa aaccgggggg
     1021 cgaatgctcc cgtggtcctt ggggggggag agccgtcttc ccgcgtcgcc ggacgccggg
     1081 cggcgggaga cgattcggct ggattgcaag cccaccacgg aattggagtc cctcgccctg
     1141 gaccttttca aggattgcct tcccgaatcc ctcgtcgaaa actaccaaag gacggtggcc
     1201 ctggtggagc ggcgccttcc gtcgcccgtc cgagcgatcg tttcgggaaa cgggtggcat
     1261 tgccacgaat cgttcaaagc catggccgcc cgggaaagag agcggggcgc acggctcatc
     1321 tccgttcagc acggaggggg gtacggcgtg cac
//
LOCUS       DAOQLS010000662         1349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_83045_length_1349_cov_3.544049, whole genome shotgun sequence.
ACCESSION   DAOQLS010000662 DAOQLS010000000
VERSION     DAOQLS010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83045_length_1349_cov_3.544049"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK362_10415"
     CDS             <1..205
                     /locus_tag="PK362_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75280.1"
                     /translation="EESIKDPDCPTMLKNILANMYRKQGNDRRAAEIFLDILDNARDR
                     SYADLALQKLQEIEKSLKSRKRL"
     gene            255..668
                     /locus_tag="PK362_10420"
     CDS             255..668
                     /locus_tag="PK362_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75281.1"
                     /translation="MISVPLLHWILGALPPGFLTAALAMEIAHYVWPRHRRWLYPSAS
                     WLLWVGTGGIAMAVASGFFLWSPAGDTLEGTHHQFLGFWILTVLAVLSLWRGLVKRRA
                     DPLFTLVWLAAAVVLAAVLRDGLALTHPSFFGGGS"
     gene            677..1054
                     /locus_tag="PK362_10425"
     CDS             677..1054
                     /locus_tag="PK362_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75282.1"
                     /translation="MGDTKKRLLVIEDDPALMELMLDYCEIIDCAPLPAENGGDGLAK
                     AVAEKPDAITVDHRLPDMTGLEVIKRLKSDPATRDIPVVFLSADAKLHETEAKALGAF
                     GVLLKPVSPAALRAVLVEALGEW"
     gene            1048..>1349
                     /locus_tag="PK362_10430"
     CDS             1048..>1349
                     /locus_tag="PK362_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNC75283.1"
                     /translation="MVKAPTGTNPLLENSLLLRACRGLSTERPPVWLMRQAGRYQSTY
                     RAIRSRVSMLELCKTPDLVARVTVDAVQQLGVDAAILFSDLLLPVEPMGLSLEYAEG"
BASE COUNT          242 a          433 c          405 g          269 t
ORIGIN      
        1 cgaggaaagc attaaggatc ccgattgccc cacgatgctg aaaaacatcc tggccaacat
       61 gtaccgtaaa caaggcaacg accgccgcgc cgcggaaatt tttctcgaca ttctggacaa
      121 cgcgcgggac cgaagctacg cggacctggc ccttcaaaag cttcaggaaa tcgagaaaag
      181 tttaaaatcc cgaaaacgcc tataagatcg gccgcccggg ccgaccggcg ttaaaactgg
      241 cataatttct ctctgtgatc tccgtgcctc tcttgcattg gatcctgggc gccctgcccc
      301 ccggcttttt gacggccgcg ctggccatgg aaatcgccca ttacgtttgg ccccgtcacc
      361 ggcgctggct ttatccgtcg gccagctggt tgctctgggt cggcacgggg ggaattgcca
      421 tggccgtcgc gagcggtttt tttctttggt cgccggccgg ggacaccctg gagggaaccc
      481 atcaccaatt tttggggttc tggattctga ccgtgctcgc cgtgctctcg ctctggcggg
      541 ggctggtcaa acggcgggcc gatcccctct ttacgttggt gtggctggcg gcggcggtcg
      601 ttttggcggc cgttctccgc gacggactgg ccctgaccca tccgtccttt ttcggcggcg
      661 gttcctagga ggccccatgg gcgatacaaa aaaaaggctt ttggtcattg aggacgatcc
      721 ggccctgatg gaactcatgc tcgattattg cgaaatcatt gactgcgccc cgctgccggc
      781 cgaaaacggt ggagacggtc tggccaaagc cgtcgcggag aaacccgacg cgatcaccgt
      841 ggatcaccgg ctgcccgaca tgaccggcct cgaggtgatc aaacggctca aaagcgaccc
      901 cgccacccgg gacattcccg tcgtcttcct atccgccgac gccaaactgc acgagacgga
      961 agccaaggcg ctgggcgcct tcggcgtcct gctcaaaccc gtgtcgcccg ccgccctgcg
     1021 cgcggtcttg gtggaggccc tgggggaatg gtgaaagcgc cgacggggac aaaccccctt
     1081 ttggaaaaca gccttctcct gcgcgcctgc cggggattgt cgaccgaacg tccgcccgtt
     1141 tggctgatgc ggcaggccgg acgctatcaa tcgacctacc gggccattcg gtcccgggtc
     1201 tccatgctcg agctatgcaa aacccccgat ctcgtggccc gagtgaccgt ggacgccgtg
     1261 cagcagctgg gcgtggacgc ggccattctc ttttccgacc tcctcctccc ggtggaaccg
     1321 atggggttgt ccctggaata cgccgaggg
//
LOCUS       DAOQLS010000663         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_83172_length_1348_cov_3.726218, whole genome shotgun sequence.
ACCESSION   DAOQLS010000663 DAOQLS010000000
VERSION     DAOQLS010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83172_length_1348_cov_3.726218"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="PK362_10435"
     CDS             complement(<1..1312)
                     /locus_tag="PK362_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75284.1"
                     /translation="MSLSPGARAVARRLARTLRWARILSGVGGAAAALGPGLLVFAGT
                     LWGLRVLAAFGELTAAARTFLWGAGTLAGAGLIVDFARRVPWGLRGVAARLARPGHPR
                     DEVWTAWELSRRERGGEALELLLASVPVPSLSAAAPRLWPAAWRGRWLAGIAGLALLA
                     ILARWAPGASADFWPFGGDAVPGVRSIRPGSLSFPRGESVSVEVQLSGPEVPLPEPWV
                     RGTRGPWARRAWSVAEGDRCLLVLENVREETFYRLEGGGHRSAEFALTPFDPPRLKTF
                     SAEVRWPGAPAERAPNPFAPEVPRGVTLEFTGEWEDDVTVFRVGDAAGSLRPVDPPGP
                     RFRWTHVVDGPTILGLWAARRGDAGAPLRVAEVAVSLRDDPPPQIRWRDPPEDLRVST
                     KETVALRFEATDDRGVASVVLEGSLNDGSPRRLFEKSFGAGAGAS"
BASE COUNT          239 a          514 c          453 g          142 t
ORIGIN      
        1 ccgaggcccc cgcgccggcc ccgaaggatt tttcaaagag ccgccgcggg gagccgtcgt
       61 tcaaggaccc ttccaatacg accgacgcga cgccccggtc gtcggtggcc tcaaaacgca
      121 acgccacggt ttccttcgtg gacacccgaa ggtcttccgg cggatcgcgc caacggattt
      181 gcgggggcgg atcgtcccga agggaaacgg ccacctcggc cacgcgaagg ggagccccgg
      241 catcgccccg gcgcgcggcc cacaagccga ggatcgtcgg tccgtccacg acgtgggtcc
      301 agcggaatcg cggcccgggg ggatccacgg gccggagcga tccggcggcg tcccccacgc
      361 ggaacacggt cacgtcgtct tcccactcgc ccgtgaactc cagcgtgacg ccgcgcggga
      421 cctccggcgc gaaggggttc ggcgcgcgct cggcgggggc cccgggccac cgaacctccg
      481 ccgaaaaagt tttcagtcgc ggcggatcga agggtgttaa agcgaactcc gcgcttcgat
      541 gtcccccgcc ctccaagcgg taaaaagttt cttcccgcac gttttccagt acaaggaggc
      601 accgatcgcc ctcggcgacg ctccaggccc ggcgcgccca ggggccgcgg gttccgcgca
      661 cccagggctc cggcagcggg acctcggggc cggacaattg aacctcaacg gaaaccgatt
      721 ctccccgggg gaacgaaagg gaaccgggcc gaatcgaccg aacccccggc accgcgtcgc
      781 cgccgaaggg ccaaaaatcg gccgacgctc cgggcgccca ccgggccaat atcgccaaaa
      841 gcgccaatcc cgcgatgccc gccagccagc ggccgcgcca ggccgccggc caaaggcgcg
      901 gcgcggcggc ggacagggag ggcaccggga cggaggccag gaggagttcg agggcctcgc
      961 cgccgcgttc ccgtcgggaa agttcccagg ccgtccacac ttcgtcccgg gggtggccgg
     1021 gccgggccaa tcgggccgcg acgccgcgaa ggccccaggg gacccgtcgg gcaaaatcga
     1081 cgatgagacc ggcccccgcc agcgtcccgg cgccccaaag gaacgtgcgc gccgccgccg
     1141 tcaattcgcc gaacgccgcc aggacgcgga ggccccacag cgtcccagcg aaaaccagga
     1201 gacccgggcc cagggccgcg gccgcgccac ccaccccgga aaggattcgg gcccaacgca
     1261 atgtccgggc caaccgtcga gcgacggccc gcgcccccgg ggaaaggctc accgttcgcc
     1321 ccttcgtttt cgaaccaccc attccagg
//
LOCUS       DAOQLS010000664         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_83221_length_1348_cov_2.463264, whole genome shotgun sequence.
ACCESSION   DAOQLS010000664 DAOQLS010000000
VERSION     DAOQLS010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83221_length_1348_cov_2.463264"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK362_10440"
     CDS             <1..440
                     /locus_tag="PK362_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75285.1"
                     /translation="YYLAGNNLGVQEDRKVDGNDVERRNRVTAVMGYDFGPLTLYGGL
                     VRSRGGGGVKIRPLPAASPWNRRVELEGEAYDFGRDEAYQGYTFNKPVYNAGARVKIV
                     EPWLWVGAQMEDIAVRRNLNLNFNMVFRDEDFAFLLAFIGLAR"
     gene            564..1085
                     /locus_tag="PK362_10445"
     CDS             564..1085
                     /locus_tag="PK362_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNC75286.1"
                     /translation="MFQEARIYSLANVGGQAVLVLEEVNGPRLLPVWIGLYEGGSIGM
                     ALAGQKFPRPLTHDLMLDAIKKLGAKLEKIVITGLKDSTFFAEVHIKRDGESIVLDAR
                     PSDSIALAVRENAPLFVAEDVFAACPELLKPISGEEVEDFKKKLETMTPEDFFRDLKK
                     KEGESGKPEGEKP"
BASE COUNT          294 a          324 c          413 g          317 t
ORIGIN      
        1 actactattt ggccggcaac aatttgggcg tccaagaaga tcggaaggtg gatgggaacg
       61 atgtggaaag gcgaaaccgg gtcaccgcgg ttatgggata tgatttcgga ccgttgaccc
      121 tctacggggg cctggtgcgc tcccgcggag gcggcggggt gaaaatccgt ccactgcccg
      181 cggccagccc atggaaccgt cgcgtggaat tggaaggcga agcctatgat ttcggacggg
      241 acgaagcgta tcaaggttat acgttcaata agcccgtcta taacgccgga gcgcgggtga
      301 aaattgtgga accctggctg tgggtgggtg ctcagatgga agacatcgcg gtgcggcgga
      361 atttaaattt gaactttaac atggtgttcc gcgacgaaga ttttgcgttc ttgctggcct
      421 ttatcggcct ggcgcggtaa ccggaattct ccggttggca cgaaccccgc gtcgactggg
      481 tgtggggcag aattatctta cacgtgtgga tgacggttgt ggttcagata aattaattga
      541 ggtaattcag ttaggaggat tttatgtttc aggaagcgcg catttattcg ttggccaacg
      601 tgggtggcca ggcggtgttg gtgttggaag aggtcaacgg tccgcggctc cttcctgttt
      661 ggatcgggtt gtatgaagga gggtccatcg gcatggcgtt ggccggccaa aaatttcccc
      721 gccccttgac ccacgacttg atgttggacg ccattaagaa attgggcgcg aaattggaaa
      781 aaattgtcat cacgggcctg aaagacagca cctttttcgc cgaggtgcac atcaaacggg
      841 acggcgaatc gatcgtgctc gacgcccgcc cgtcggattc catcgccctg gccgtgcgcg
      901 agaacgcccc cctctttgtg gccgaggacg tttttgccgc ctgccccgaa ctcttgaaac
      961 ccatctccgg cgaagaggtc gaagacttca aaaagaaatt ggaaaccatg acccccgaag
     1021 atttcttccg cgacctaaag aaaaaagaag gcgaaagcgg caaacccgaa ggcgagaaac
     1081 cgtagggttt tgacgttttt tgttttggtg ttgggggtgt gggatttttt aggaattttt
     1141 gtaactactc accaccctca tccggccctt cgggccacct tcccccaaga ggggaaggaa
     1201 aacagagatt ttccctctcc tcttgggaga gggcagggtg agggagctga gtagttacga
     1261 attttttggt ttttcgctcc cccattgagg cgattcgagc acgattttgt gacgatcgcg
     1321 gggctgaccc tgtccgatcc ggcccgta
//
LOCUS       DAOQLS010000665         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_83671_length_1344_cov_4.833204, whole genome shotgun sequence.
ACCESSION   DAOQLS010000665 DAOQLS010000000
VERSION     DAOQLS010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83671_length_1344_cov_4.833204"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK362_10450"
     CDS             complement(<1..289)
                     /locus_tag="PK362_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75287.1"
                     /translation="MTALVEDLEKLKSEAEGELMSLDTSEAFDAFRLKYLGRSSGRLT
                     VLLQALPGLPPEERRDVGRRANELKNHLEKSLAEKKKALDDRRLSASLTRDL"
     gene            complement(327..692)
                     /gene="rplT"
                     /locus_tag="PK362_10455"
     CDS             complement(327..692)
                     /gene="rplT"
                     /locus_tag="PK362_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNC75288.1"
                     /translation="MRVKSVVYTRQRKKKWFRRAKGAYATKKSRWRMVLQHLEMSMRH
                     MYRGRKQKKGDLRAVWIQRINAAARQEGLSYSRFLSGLKKAGVVLDRKMLANLASADP
                     ASFKQLANVARENLSVKAA"
     gene            complement(682..882)
                     /gene="rpmI"
                     /locus_tag="PK362_10460"
     CDS             complement(682..882)
                     /gene="rpmI"
                     /locus_tag="PK362_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNC75289.1"
                     /translation="MPKIKSHSGAKKRFRVTANGLVMHQTQGRRHLLIGMSSNRRRRF
                     RKDNKLNKIQSKIIRTLLPYAR"
     gene            complement(891..1328)
                     /gene="infC"
                     /locus_tag="PK362_10465"
     CDS             complement(891..1328)
                     /gene="infC"
                     /locus_tag="PK362_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010532430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNC75290.1"
                     /translation="MFVKTLDEAIRISQDRGKDLVEVAPLAQPPVCKVVDFSKYRYEK
                     EKQKKEARKHQKGGHVKEIRFRPQIGEHDFVTKLKAVQKFLEDRDKVRITVMFRGREM
                     EHKDLGQRIIERLKDHLGELATVESNPMMMGNRMILMLAPQKK"
BASE COUNT          238 a          371 c          391 g          344 t
ORIGIN      
        1 ggaggtcccg ggtgagactc gccgagaggc ggcggtcgtc cagggccttt ttcttttcgg
       61 cgagggattt ttcgaggtgg tttttgagtt cgttggcccg tcgcccgaca tcccgccgtt
      121 cctcgggagg caacccggga agggcttgaa gaagtacggt caaacgaccc gaggaccgcc
      181 cgaggtactt cagccggaag gcgtcgaacg cttcggaagt gtctagggac atcaactcgc
      241 cctcggcctc gctcttgagt ttttccaaat cctccacaag cgcggtcatg ggcggttgat
      301 cgccggcggt ccgggatccg agggggttag gcggctttaa cggagaggtt ttcccgcgcg
      361 acgttggcca attgcttgaa cgacgccgga tcggcggagg ccagattggc gagcatcttg
      421 cggtccagaa ccacgccggc ttttttcagg ccggacagga aacgggagta actcaggcct
      481 tcctgacgcg cggcggcgtt aatgcgctga atccaaacgg cgcgcagatc gccttttttc
      541 tgtttgcggc cccggtacat gtgccgcatg gacatttcca aatgctgcaa taccatgcgc
      601 caacgggact tcttggtggc gtaggctcct ttggctcgac ggaaccactt cttttttcgt
      661 tgacgggtgt aaacgacgct tttaacgcgc atagggcaag agtgtcctga taattttgga
      721 ttggattttg ttcagcttgt tgtctttgcg aaaccggcga cgacggttgg acgacatgcc
      781 gatcaacaaa tgccggcgac cctgggtctg gtgcatgacc aggccgttgg ccgtcactcg
      841 gaaccgtttt tttgcaccgc tgtggctttt aattttgggc atgatccgtc ctattttttc
      901 tggggcgcca gcatgaggat catccgattc cccatcatca tggggttcga ttccaccgtg
      961 gccagctcgc ccaaatgatc cttcaggcgt tcgatgatcc gttgtccgag gtccttgtgt
     1021 tccatttccc ggccccgaaa catcaccgtg atccgaactt tgtcacggtc ttccaaaaat
     1081 ttctgcacgg ccttcaactt ggtcacgaag tcgtgctcgc cgatttgtgg acgaaaccgg
     1141 atttccttca cgtgcccgcc cttttgatgc tttcgggctt ccttcttctg tttttctttt
     1201 tcgtagcggt acttcgaaaa gtccaccact ttgcaaacgg gcggttgggc caggggggcc
     1261 acttccacca aatctttgcc gcgatcctgg gagatgcgaa tcgcttcgtc gagggtcttg
     1321 acaaagattt ggtggaagtg gccc
//
LOCUS       DAOQLS010000666         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_83807_length_1343_cov_4.792702, whole genome shotgun sequence.
ACCESSION   DAOQLS010000666 DAOQLS010000000
VERSION     DAOQLS010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83807_length_1343_cov_4.792702"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            675..1088
                     /locus_tag="PK362_10470"
     CDS             675..1088
                     /locus_tag="PK362_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75291.1"
                     /translation="MFSQSRCFHRRRAARGFGFSFRPSVPFSIFIVGVLRSIPFDISI
                     LSVNGGAAVVAIGQHITPSNTAHIIKGQTIPSYFARGIFSLFPSRTAARRARYEHFVE
                     ITEMLIRVFWRVLHPCRVRNRASWRNVVRRFSVEN"
BASE COUNT          151 a          410 c          491 g          291 t
ORIGIN      
        1 gggccgccga aaattccgac gggaaaacgt cgcccaccgc caagcggggc gcgtgcggcg
       61 cgcgaagtgc gccgcgtgcg cggccttttc tccatgacag agcccccggc ggggggtgga
      121 ggggggaccc gccgggggct ttccgccgcg atagcggcgc tcctctcccg gcgcggcgac
      181 gcgccggggg tttttcggga gccgaccggg gtggtgtttc cgggcggccc ggctccgccg
      241 gggtttgtca gttcccaggc cgcgcacgcg ggaatcactc cggcggcgcc ggacgcgaaa
      301 cgcgcggggg gttttccgtt cacccaccag ggcggcggct tttccagccc cggggttttt
      361 cagcgccacg gcccgggggt ttttccagtc ccggcggcgc gccgggtttt ggttttggat
      421 cctccgggcg cgcggccgcg cggggtgttt tcgttgcggc cccgtcggtg ttttccggcg
      481 cgcggtgatc tggttttcat tccgtcgcca tcgtgggggt ggcggtgttt tttcggtgtc
      541 ccagacggtg ccatttcgac gtcgcggcgc ggtcgggcgg tgtctttttc cgtcgggggt
      601 cgccgcgcgc cgaccacctg gtcgtccagt tccccgcagg tttgaagtgg tcgccaccgg
      661 cgcggcgggt tttggtgttt tctcagtcgc ggtgttttca tcgccgccgc gcggcgcgcg
      721 gttttgggtt ttcctttcgt ccttccgttc cattttccat ttttatcgtc ggtgttcttc
      781 gttccattcc tttcgacatt tccattttgt cggtgaacgg cggcgcggcc gtcgtcgcca
      841 ttggtcagca catcacgcca agcaacacgg cacacatcat taaaggtcag acaataccgt
      901 cttattttgc gcgcggcatt ttttcgcttt ttccgtcgag aacggcggcg cgccgggcgc
      961 gatatgaaca tttcgttgaa atcaccgaaa tgctcattcg cgtcttttgg agggtgctac
     1021 acccttgccg ggtcagaaat cgtgcgtcct ggcgaaacgt agttcgtcgg ttttccgtcg
     1081 agaactgaaa aagggccaaa gggtcggcgg ttgggggcgt tacttttttg gggccgccgc
     1141 caggcggccc cgggggcgcg agtgaggccc cggcccgcgg aagcgggccg gggccggggc
     1201 gccaggccag gcaaaaaacg ggcggggggt ggaggggggc cccgcccgtt ttatgcctgg
     1261 ccggtttgga caggccgggc cagaggccgc gccgcgcggg ggtggagggg gccccgcgcg
     1321 gtgtggcccg gtttggccct ggt
//
LOCUS       DAOQLS010000667         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_83808_length_1343_cov_4.669255, whole genome shotgun sequence.
ACCESSION   DAOQLS010000667 DAOQLS010000000
VERSION     DAOQLS010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83808_length_1343_cov_4.669255"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            772..>1343
                     /locus_tag="PK362_10475"
     CDS             772..>1343
                     /locus_tag="PK362_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75292.1"
                     /translation="MTKIERTPGSARKEANYEIPLLSALCGSDDLSDAIERRVRGRGA
                     SFDVRPLVRKRVLSWIRDEEDGGTISSDPSTWNTVRRAVGLPPLAPAALSRFRFKPLS
                     SMRRRLEERRFQWARERERTLRANPAVPGSRKKVLSKAAGLRKVFNQMGATVESLEVN
                     FLDRYPFYLKDLGMNTPANRRRFFHHAVVFL"
BASE COUNT          248 a          360 c          436 g          299 t
ORIGIN      
        1 gctccacttt aaggaagaaa gccgggccag aaattggccc ggctttctgt ttaaaagtgc
       61 gggtagcccc ggcggttcgg gccggggctt cacccggttg ctccatccta tatttttaag
      121 gaagaaagcc gggccagaaa ttggcccggc tttctgttta aaagtgcggg tagccccggc
      181 ggttcgggcc ggggcttcac ccggttgctc catcctatat ttttaaggaa gaaagccggg
      241 ccagaaattg gcccggcttt ctgtttaaac gtgcgggtcc cacgcagagc tttgggactg
      301 acgtcggcgc agagcgccga aatctccgtc tgtagccccg gcggtttagg ccggggcttc
      361 acgatctcca cttaatatta gaaatcgccc ttgatggttt ggtcaagggc ccgagcactc
      421 catccttgac tttaaggaag aaagccgggc cagaggatgg cccggctttt tgtttaaaag
      481 tgggtgtccc acgcagagct cgtgcggggt ttcggacact ctgtggccgc cgctcgtcct
      541 cggagctctg cgaagagtat tgggactgac ggcggcgcag agcgccgaca tccccgtctg
      601 tggcctcgat tttgggaacc gggaggacat gggattggtt tatcggtata ctgtccccgt
      661 caatgggtgg tattaatttg atggggaggt cgccttatgg gagtttggga ggttccgtac
      721 gggacggcgg cgcaggccaa ggaattcctt cggcggatgc ggaagccttg gatgacgaag
      781 attgaacgga cgccggggtc cgcccgcaag gaggccaatt acgaaatccc cttgttgtcc
      841 gcgttgtgcg gatcggacga cctgagcgac gcgatcgaac ggcgagtccg cgggcggggc
      901 gcttctttcg atgtccggcc tctggtgcgg aaacgggtgc tctcctggat tcgcgacgag
      961 gaagacgggg gaacgatttc ttccgacccg tcgacctgga acaccgtgcg ccgggcggtg
     1021 ggtttgcctc ccttggcccc cgcggcccta tcccggtttc gatttaaacc gctttcatcg
     1081 atgcgtcgcc gcttggagga gcgccgtttt caatgggcgc gggagcggga gcgaacgctt
     1141 cgagcgaacc cggcggttcc gggaagccga aaaaaagttc tttcaaaggc cgccggcctg
     1201 cgtaaagtct tcaatcaaat gggggcgacg gtggagtcgc tggaagtgaa tttcctggac
     1261 cgatacccgt tttatctcaa ggatttggga atgaacacgc cggccaaccg tcggcgcttt
     1321 tttcaccacg ccgtggtttt cct
//
LOCUS       DAOQLS010000668         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_83875_length_1343_cov_2.529503, whole genome shotgun sequence.
ACCESSION   DAOQLS010000668 DAOQLS010000000
VERSION     DAOQLS010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83875_length_1343_cov_2.529503"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PK362_10480"
     CDS             <1..778
                     /locus_tag="PK362_10480"
                     /inference="COORDINATES: protein motif:HMM:NF018731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1338 family protein"
                     /protein_id="HNC75293.1"
                     /translation="FRTLATQNPASGYFVLSRIFEALGYVPQACYEFPDKHFSSIHYA
                     YPKEGFPKLFITQLKTWELSAPVRALLLKTAKGARAPLVDADLASLRALTDRDGAWTP
                     ALFQRTTNVFLKLPWPLPQRRDVEAVNRESQFGAWVMVHGHGVNHFTASVDSHRVEAL
                     NDIEKVVAALRSAGIPMKKEIEGARGSKLRQSATEAVVLPTLMKEGRKTVRVPWPFAY
                     MEIAERPLIVNPSTGRPERFEGFLGPQATQLFEMTKRPKQ"
     gene            complement(826..1068)
                     /locus_tag="PK362_10485"
     CDS             complement(826..1068)
                     /locus_tag="PK362_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNC75294.1"
                     /translation="MEPKIAQKSPCVQEEEPGKKFWCACGHSENQPYCDGSHARSGTG
                     LRPLSIEITEKKTVAWCGCKHSKNKPFCDGTHKTLP"
     gene            1154..>1343
                     /locus_tag="PK362_10490"
     CDS             1154..>1343
                     /locus_tag="PK362_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC75295.1"
                     /translation="MSLPVEISASDSAALLKADPSVKLIDVRTDAEYLVARIDGALLV
                     NNADSMAKVLSWPKETRIL"
BASE COUNT          252 a          395 c          398 g          298 t
ORIGIN      
        1 ctttcgcacc ctggccaccc aaaatcccgc ttcgggctat ttcgtcctgt cgcggatttt
       61 tgaggccctc ggatacgtgc cccaggcctg ttacgaattt ccggacaagc atttcagctc
      121 catccactac gcctatccga aggaggggtt ccccaagctg ttcatcacgc agttaaagac
      181 ctgggaattg tccgccccgg tccgggccct tcttttaaaa acggcgaagg gcgctcgggc
      241 gcccctggtg gacgcggacc tggcttccct tcgggccttg accgaccggg acggcgcgtg
      301 gacgccggcg ctttttcaac ggacgacgaa cgttttcttg aaattgccct ggcctctccc
      361 ccagcgacgg gacgtcgaag ccgtcaatcg ggaaagccaa tttggggcct gggtgatggt
      421 ccacggccac ggcgtcaatc attttacggc gtcggtggat tcccatcggg tggaggcatt
      481 gaacgacatc gaaaaggtgg tggcggcgtt gcggtcggcg ggtattccca tgaaaaaaga
      541 aatagaaggc gcccgggggt ccaaacttcg tcaatccgcg accgaggccg tcgtccttcc
      601 cacgctcatg aaggagggcc gaaagaccgt ccgggttccg tggcccttcg cctatatgga
      661 aatcgccgaa cgccccctga tcgtgaatcc ctcgaccggc cggcccgagc gtttcgaagg
      721 ttttctcggc ccccaggcca cccaattgtt cgagatgacc aagcggccga aacaataaaa
      781 aaccccggac ccgtgggggg cccggggttt ggggaacgtt gaaggtcagg gcaaggtctt
      841 gtgggtcccg tcgcagaagg gcttgttttt ggagtgtttg cacccgcacc aggccacggt
      901 tttcttttcg gtgatctcga tggagagggg gcgaaggccc gtgccggagc gcgcgtggct
      961 tccgtcgcaa tagggttggt tttcggaatg cccgcaggcg caccaaaact tctttcccgg
     1021 ttcttcttcc tggacgcagg ggcttttctg ggcgattttg ggttccatgg ttcctcctgg
     1081 agtgtctccg cccatcatat ctgattgtaa aatccttcag gaacttattc cgccgaagtg
     1141 gggttatata aatatgagtt tgcctgtgga aatcagcgcc tcggattccg ccgccctatt
     1201 aaaagcggat ccctcggtta aattgatcga tgtccgaacc gacgccgagt acctggtggc
     1261 gcgcattgat ggggcccttc tcgtgaacaa cgccgactcc atggcgaagg tcctctcctg
     1321 gcccaaggaa acccggattc tcg
//
LOCUS       DAOQLS010000669         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_85302_length_1331_cov_4.344044, whole genome shotgun sequence.
ACCESSION   DAOQLS010000669 DAOQLS010000000
VERSION     DAOQLS010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85302_length_1331_cov_4.344044"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PK362_10495"
     CDS             <1..829
                     /locus_tag="PK362_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNC75296.1"
                     /translation="RGIFDNMRPFLRANGFDRFIEQKDFARPTFTTAWGVCDEDIFDR
                     ALEEFDAQHATGRRFFAAVLSVSNHKPYTYPAGRIDLDPQRRRREHAVKYADYALGRF
                     FDKARERPFFKNTVFVVIGDHGARVYGADFIPIRSYEVPFLIYAPGMLKPKKVDVLGS
                     SLDIAPTLMGLTNMHYQSVFFGRDLWDVDPATSYALMQHDRDVGILREGRLAVLGLHR
                     TARVFKYDRASKFFSASLPLGPEETELVRDGVALYQTAYDLYQNRRYHVPDAPVLVR"
     gene            807..>1331
                     /locus_tag="PK362_10500"
     CDS             807..>1331
                     /locus_tag="PK362_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC75297.1"
                     /translation="MPPSSSAELPGGETLCPHFGACGGCATQNAPYAEQLAAKEARVR
                     GLLAPFSADEFRPIRPSPDVFHYRNKMEFAFGGLKDGPVLLGLRQKGRFDRVVDLTEC
                     RLLSTAAGPLMAAVRGWAVVHGLPTYHLKSHKGFLRYLVVREGKNTGQRLLNLVTAEG
                     DAPGSERAAPSELQH"
BASE COUNT          226 a          440 c          403 g          262 t
ORIGIN      
        1 gcggggaatt ttcgacaaca tgcggccttt tctccgggcc aacggattcg accggttcat
       61 cgaacaaaag gatttcgccc ggccgacctt cacgacggcc tggggcgtct gcgacgagga
      121 tatctttgat cgggccttgg aggaattcga cgcccaacac gccaccggtc gccgattttt
      181 cgccgccgtc ctttccgttt cgaaccacaa gccctacacc tacccggccg gccgcatcga
      241 tttggacccc cagcgccgcc ggcgggaaca cgccgtcaaa tacgcggatt acgcccttgg
      301 ccggtttttc gataaggctc gggagcggcc gtttttcaag aacaccgttt tcgtggtgat
      361 cggtgatcac ggcgcccggg tctacggggc ggatttcatt cccatccggt cctacgaagt
      421 ccccttcctg atctacgccc cggggatgtt gaaaccgaaa aaagtggacg tgttgggttc
      481 gtccttggac atcgccccga ccttgatggg gttgaccaac atgcattacc aaagcgtttt
      541 cttcgggcgg gatttgtggg acgtggaccc cgcgaccagc tacgccctca tgcaacacga
      601 ccgcgacgtg ggcattttac gggagggacg gctcgcggtc ctgggacttc accggacggc
      661 gcgcgttttc aaatacgatc gggcgtccaa attcttttcc gcctctctgc ccctgggccc
      721 ggaggagacc gagttggtgc gcgacggcgt ggcgctttac caaacggcgt acgatctgta
      781 ccagaaccgc cggtatcacg tgccggatgc ccccgtcctc gtccgctgaa ctcccgggcg
      841 gggaaaccct ctgcccccat ttcggcgcct gcggggggtg cgccacccaa aacgcgccct
      901 acgccgagca actcgccgcc aaggaggccc gggtgcgcgg cctcctcgcg cctttttccg
      961 ccgacgaatt tcggccgatc cggccctctc ccgatgtttt tcactaccgc aacaaaatgg
     1021 aattcgcctt cggcggactc aaggacgggc cggtccttct cggccttcgc caaaaaggcc
     1081 ggttcgaccg ggtggtcgat ctgaccgagt gccgcctcct gtcgacggcc gccggccccc
     1141 tgatggcggc cgtccggggt tgggcggtcg tccacgggct gccgacctac cacctgaaat
     1201 cccacaaggg atttttgcgc tatttggtgg tccgggaagg gaaaaacacg ggccaacgcc
     1261 tgttgaattt ggtgacggcg gagggcgacg cccccgggag cgagcgtgcg gcgccttcgg
     1321 agctccagca c
//
LOCUS       DAOQLS010000670         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_85568_length_1329_cov_3.459184, whole genome shotgun sequence.
ACCESSION   DAOQLS010000670 DAOQLS010000000
VERSION     DAOQLS010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85568_length_1329_cov_3.459184"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="dnaA"
                     /locus_tag="PK362_10505"
     CDS             complement(<1..842)
                     /gene="dnaA"
                     /locus_tag="PK362_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNC75298.1"
                     /translation="MTVAPPDSAQGSGDTRLIPSYTFEEFVIGPHNRFPHAAALAVSE
                     NLGKAYNPLFIYGPVGIGKTHLMQAVGHRVLQRDAKTRVLYVTAQQFMSEVIELLQAG
                     NLNALRERYRALDLLLVDDIQFLATSEATQEEFFHMFNDLHSAGKQIIMTSDRPPKML
                     TTLEDRLRSRFEWGLIADIKHTNLETRVAILRKKEGHLKGVRLADDIRIYIASRLKSN
                     IRELEGFLRRVQAYSQLNNQDVTLVLVKEIMKELLPPEEWVDDPVAEEPAAPAEEIAL
                     PPELP"
     gene            complement(904..>1329)
                     /locus_tag="PK362_10510"
     CDS             complement(904..>1329)
                     /locus_tag="PK362_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75299.1"
                     /translation="REPKLVVELKTPEDSTFKTAIFFPQGGDAPKQKFLNTITDMAQK
                     KSKKPMFFHIVHSQATSITSDNAVEWIWTAKAQGADCFFVILPPDILPDFMEGIVAEA
                     RQAGLRCFLIPQAEIGSKLLYMDLMVELMLIKRKPKTNP"
BASE COUNT          263 a          354 c          408 g          304 t
ORIGIN      
        1 ggcaattccg gcgggagcgc gatttcttcg gcgggggcgg cgggctcctc cgccacggga
       61 tcgtccaccc attcttcggg cggcagcaat tccttcataa tttccttcac caaaaccaag
      121 gtcacgtcct ggttgttgag ctgggaatag gcctgtaccc ggcgcaaaaa tccttccaat
      181 tcccggatgt tggatttgag gcggctggcg atgtaaattc ggatatcgtc ggcgagtcga
      241 acgcccttca agtgcccttc cttctttcga agaatggcca cgcgggtttc caaattcgtg
      301 tgtttgatgt cggcgatgag gccccattcg aagcggcttc gaaggcggtc ttcgagggtg
      361 gtcaacattt tgggcggacg gtccgacgtc atgatgattt gctttccggc ggaatgcaaa
      421 tcgttgaaca tgtggaaaaa ctcctcctgg gtcgcttcgg aggtggccag aaactggatg
      481 tcgtcaacca gaagcagatc cagggcgcgg tagcgctccc gaagggcgtt caggtttccg
      541 gcctggagca gctcaatcac ttccgacatg aactgctggg ccgtgacgta gagcacccgg
      601 gtcttcgcgt cccgttggag cacgcggtgg ccgacggctt gcatcaaatg cgtttttccg
      661 atccccacgg ggccgtaaat aaagagcggg ttgtacgcct tgcccagatt ttcggaaaca
      721 gccaacgccg ccgcgtgggg aaagcggttg tgcggcccaa tgacgaattc ctcaaacgtg
      781 taggacggga tcaggcgggt gtcgccggag ccctgggcgg aatcgggggg ggccacggtc
      841 accgcttgcc ctttccggcc gcggaggccc gtaaagcccg gtggtgaatc aattcaaaaa
      901 aggtcatggg ttggttttcg gtttcctttt aatcagcatg agttccacca ttaaatccat
      961 gtaaagcaat ttggagccga tttcggcctg gggaatgaga aagcaacgca ggccggcctg
     1021 ccgggcttcc gccacaattc cctccataaa gtccgggaga atgtccgggg gcagaatgac
     1081 gaagaaacag tccgccccct gggctttcgc cgtccaaatc cattcgacgg cgttgtccga
     1141 ggtgatgctg gtggcctggg aatggacgat gtggaagaac atgggctttt tcgatttctt
     1201 ttgggccatg tccgtgatcg tgttgagaaa tttctgtttc ggcgcgtcgc ccccctgggg
     1261 aaaaaagatc gccgttttga acgtggaatc ctcgggggtt ttcaactcca cgaccagttt
     1321 gggttccct
//
LOCUS       DAOQLS010000671         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_85695_length_1328_cov_3.023566, whole genome shotgun sequence.
ACCESSION   DAOQLS010000671 DAOQLS010000000
VERSION     DAOQLS010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85695_length_1328_cov_3.023566"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK362_10515"
     CDS             complement(<1..234)
                     /locus_tag="PK362_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC75300.1"
                     /translation="MRPGRVFLVGAGPGDPGLLTLRGAALLKSADVVVLDALVDRRLL
                     GHCRKETTVIDAGKRGHGRVLMRQGEINRLLVRR"
     gene            complement(231..>1328)
                     /locus_tag="PK362_10520"
     CDS             complement(231..>1328)
                     /locus_tag="PK362_10520"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNC75301.1"
                     /translation="TAPTAPPAPSRARAFLAGLALGIVLALTGALAGLWAANGRLSAG
                     FERGLRRFVFTDGNTAGFESATLDLWRGRAALRGVRFQGPEGLRGTVDRVTLSAPPAE
                     LFSGAGPVAVVGLERPALRWGDAARSGAEFRAPALPGVVREWTVTDGSGTWTLADAGL
                     TLDRWNLRLVRSDPGTSGRSVLTLDGAGRVLPEDPAAATVHVTFEEPFSPAALEGEIQ
                     IRDLSLPALYRTWGRDPELQILSGRLDVKTQFTIGGDQLTASHLVEIKDLRVDPGARK
                     KLFGLSVKRLKDVLVIDRLSFVVPMSGSVQDPQIGFATSLEQILTKILDGKIDDPEDL
                     RTWARRGGNYFGAKADAAWRAWLKRRRERAS"
BASE COUNT          245 a          471 c          427 g          185 t
ORIGIN      
        1 gcgccgaacc agcaaccgat tgatctcccc ctggcgcatc agcacccggc cgtgaccccg
       61 tttgccggcg tcgatgaccg tggtttcttt tcgacaatgg cccaacaacc gtcggtccac
      121 caacgcgtcc aaaaccacca cgtcggccga cttcaacagg gcggcgcccc gaagggtcaa
      181 taacccggga tcccccggac ccgcgcccac caggaaaacg cgccccggcc tcacgaggcc
      241 ctttcccgac ggcgcttgag ccacgcccgc caggcggcgt cggccttggc gccgaaatag
      301 ttgccccccc ggcgggccca ggttcgaagg tcctccggat cgtcgatttt tccgtccagg
      361 atcttcgtga gaatctgttc cagggaggtg gcaaacccga tttgggggtc ctgcacggac
      421 ccgctcatgg ggaccacgaa ggagaggcgg tcgatgacca aaacgtcttt cagacgtttg
      481 accgacaagc cgaaaagttt tttccgggcg cccggatcca cccgcaggtc tttgatttcc
      541 accagatggc tggccgtcag ttggtcgccg ccgatcgtga actgggtttt gacgtcgagc
      601 cgaccggaaa gaatttgcaa ttcgggatcc cggccccagg tccggtagag ggccggcagg
      661 gacagatcgc ggatttggat ttcgccttcc agagccgccg gggaaaaggg ttcctcaaac
      721 gtgacgtgaa cggtcgcggc ggccgggtcc tcggggagaa cgcgcccggc gccgtcgagg
      781 gtcaaaaccg accgcccgct cgtccccgga tcggaacgga ccagccggag attccaccga
      841 tccaaggtca accccgcgtc ggcgagcgtc caggtgccgg aaccgtcggt cacggtccac
      901 tcccggacga cccccggcag ggcgggcgcg cgaaattccg cgccgctccg ggcggcatcg
      961 ccccatcgaa gcgccgggcg ctccaagccc accacggcga cgggcccggc gccggaaaac
     1021 aattccgccg ggggggccga gagggtcacc ctgtccacgg tgccccggag cccttcgggc
     1081 ccttgaaaac ggactccccg aagcgccgcc cgtccgcgcc acaaatccag ggtggcgctc
     1141 tcaaaaccgg cggtgtttcc gtcggtaaat acgaaacgcc gaagcccccg ctcgaacccc
     1201 gccgacaacc gtccgttggc cgcccagagg cccgccaagg cccccgtcag ggccaggacg
     1261 atgcccaaag cgaggccggc caaaaaagcg cgggcgcggg acggggcggg gggagcggtc
     1321 ggggcggt
//
LOCUS       DAOQLS010000672         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_85739_length_1328_cov_2.354281, whole genome shotgun sequence.
ACCESSION   DAOQLS010000672 DAOQLS010000000
VERSION     DAOQLS010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85739_length_1328_cov_2.354281"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PK362_10525"
     CDS             complement(<1..308)
                     /locus_tag="PK362_10525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC75302.1"
                     /translation="MVFNQPLAYRTLPPAVKSLLTITVGIFGLQWVLSLTGGLDLVGL
                     FGLTPARVTGRLWLWQTVTYMFLHGGPFHLLFNCYMLWALGKVIEVQWGTRSFLFYYF
                     L"
     gene            complement(429..>1328)
                     /locus_tag="PK362_10530"
     CDS             complement(429..>1328)
                     /locus_tag="PK362_10530"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNC75303.1"
                     /translation="FLPEFRRLGARRTRRRGRGGSPIRLDFSVQDPATDTLALDSKNR
                     WVRRAGGALLFRPGGHGALLRNLEAAGGDIVFIKNIDNVPVESRQARGNRWRLVLAGR
                     LAHAQRDAARWIAALTRGAVSEKIIRGAERFVFKTLGIQNKKDSPAERKARLLEILNR
                     PWRVCGMVPNAGEPGGGPFWVNGADGPSRQILEGSQVGENQKKFLRRSTHFNPVDLVV
                     GLKDWQGRSFPLEKFVDPNQALVSVKHFEGREIRTVEHPGLWNGAMARWNTVFVEVPA
                     GIFHPVKTILDLLRPGHRASPLR"
BASE COUNT          283 a          426 c          378 g          241 t
ORIGIN      
        1 aggaaataat agaagagaaa ggaccgcgtt ccccactgga cttcgatgac tttccctaac
       61 gcccagagca tgtagcaatt gaagagaaga tggaagggac cgccgtgtag gaacatgtac
      121 gtcacggtct gccacaacca aagacggccg gtgacccggg cgggcgtgag cccaaaaagc
      181 cccaccaggt ccaacccgcc cgtgagagac aacacccact gaagcccgaa gattccgacg
      241 gtgatcgtta aaagggattt caccgcggga ggcagagttc gataggcaag cggttgatta
      301 aaaaccatca ggcccccctt gtccgtttcc tgagagccgc acgttcctcc ccaatggggc
      361 tcaaaacgtc gccgtctggc cggccgattc cgtggacaat catgacagcg attttcattg
      421 gatttatttt accttaaagg gctggccctg tggccggggc ggagcaaatc caggatcgtt
      481 ttgaccggat gaaaaattcc ggcgggcacc tcgacgaaaa cggtgttcca gcgggccatg
      541 gcgccgttcc acaagccggg atgttccacc gtccgaatct cccgcccttc gaaatgtttg
      601 acggaaacca gggcctggtt cgggtccaca aatttctcca gcggaaagga acgcccctgc
      661 caatctttca agcccaccac cagatcgacc ggattgaagt gggtcgaacg gcgcaggaac
      721 ttcttttgat tttcccccac ctgggaccct tccaggattt ggcggctcgg cccgtcagcc
      781 ccgttgaccc aaaacgggcc gcccccgggc tccccggcat tgggcaccat gccgcagacg
      841 cgccaaggac ggttcaaaat ttccagcagg cgggcttttc tttccgccgg ggaatccttt
      901 ttattttgga tgcccaaggt cttgaacaca aaacgttccg ccccgcgaat gattttctcg
      961 gaaacggccc cgcgggtcaa cgccgcgatc catcgggcgg cgtcccgctg ggcgtgcgcc
     1021 aaccgcccgg ccaataccaa ccgccagcgg ttcccccggg cctgccggga ttccaccggg
     1081 acgttgtcga tgttcttgat gaagacgata tcccctcccg cggcctccag gtttctcaag
     1141 agagcgccat gccccccggg gcgaaaaagc aatgcccctc cggcgcggcg gacccagcgg
     1201 tttttactgt cgagagccaa ggtgtccgtg gcgggatcct gaacgctgaa gtccagccgg
     1261 atcggcgatc cgccccgccc gcggcggcgg gtccggcgcg cccccaatcg cctgaattcc
     1321 gggagaaa
//
LOCUS       DAOQLS010000673         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_86103_length_1325_cov_2.918110, whole genome shotgun sequence.
ACCESSION   DAOQLS010000673 DAOQLS010000000
VERSION     DAOQLS010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86103_length_1325_cov_2.918110"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1325)
                     /locus_tag="PK362_10535"
     CDS             complement(<1..>1325)
                     /locus_tag="PK362_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75304.1"
                     /translation="FYFGAMMLMSSLDVIAAGYFGIPVSLNPVVFNQYSLFVLANLAF
                     GALHARVYTYQDGQWEIERNPDGSPKTASWATRLSLTVRGMGIHSPLLWTNILPLLGP
                     TITILGAAATVVLHMVHNQLAAQFGWRALTVGANAPVSTGISLESELPGGGHLKTSIP
                     PEKMKQVSHSSLYLVHWLVENRFNVIRAAMAGITSRATGQDKMAVFQQLAPENIKNVN
                     VTFYATGSEQNPHKKFVCKLTVELKNGEKPLTVLIPLALKRGSPTTADNTAEDETFKE
                     IQRMKFISDLDTERGLTLDQRRVPISGGFVRVTQSYDNAADRFNFDLIDPKTAPSLGS
                     TAYYVHFQEFIEGGTIREARSRRAYTSENLKSAVKNVLSIWSDMGRQFPEDAYLWIMD
                     VNPDNFMFRRNTNGETRPNAVFVDLGLKFRRSGDPAIAAADKAAAALQK"
BASE COUNT          270 a          337 c          415 g          303 t
ORIGIN      
        1 ttttttgcaa agccgcggcg gctttgtccg cggcggcgat ggcgggatcg ccggaccgtc
       61 ggaatttaag tcccaagtcg acaaatacgg cgtttggtcg ggtttcgccg ttggtgttac
      121 ggcgaaacat gaagttgtcc gggttgacat ccataatcca caaataggcg tcttcgggga
      181 attgacggcc catgtccgac caaatgctga ggacgttttt gaccgcggat ttcaggtttt
      241 cggatgtgta agcccgcctc gaccgggctt ctcgaatggt cccgccctcg ataaattcct
      301 ggaaatgaac gtaataagcg gtgcttccca aagaaggcgc tgtttttgga tcgatcaaat
      361 cgaagttgaa tcggtcggcc gcgttgtcgt agctctgcgt gacgcggacg aaccccccgg
      421 atataggcac gcgacgctgg tccagcgtca accctcgctc ggtatcgaga tccgagatga
      481 atttcatccg ctgaatttcc ttaaaggttt cgtcttcggc ggtgttatcg gcggtcgtcg
      541 gtgaacctcg cttcagggct aagggaataa gaaccgttag gggtttctcc ccatttttca
      601 attcgaccgt aagcttgcag acgaattttt tgtgggggtt ctgttcactg cccgttgcat
      661 agaacgtgac gttcacgttt ttaatgtttt ccggcgccaa ttgttgaaac acggccattt
      721 tatcctgccc ggtggctcgg gaagtgatgc cggccatggc ggcccgaata acgttgaagc
      781 ggttttccac cagccagtga acgaggtaaa gcgaactgtg gctcacttgc ttcatttttt
      841 caggtgggat cgaagttttt aagtggccgc cacccggtaa ttcggattcc aagctgatgc
      901 cggtgctcac cggggcattg gcgccgaccg ttaaggcgcg ccagccgaat tgggcggcca
      961 gctggttgtg gaccatgtgg aggacgacgg tggccgcggc gccgaggatc gtgatggtgg
     1021 gacccagcag cggcaggatg ttggtccaaa ggagagggga gtgaatgccc atgccgcgca
     1081 ccgtcaggct caggcgcgtg gcccaggagg cggtcttcgg cgatccgtcc gggttccgct
     1141 caatttccca ttggccgtcc tggtacgtgt aaacccgggc gtgcaaagcc ccaaaggcga
     1201 ggttggccag gacgaacagg gaatattggt tgaagacgac cgggttaagg gagacgggaa
     1261 tcccgaaata gccggcggcg atgacgtcga gggaggacat cagcatcatc gcgccgaaat
     1321 agaac
//
LOCUS       DAOQLS010000674         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_86393_length_1323_cov_2.306782, whole genome shotgun sequence.
ACCESSION   DAOQLS010000674 DAOQLS010000000
VERSION     DAOQLS010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86393_length_1323_cov_2.306782"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PK362_10540"
     CDS             complement(<1..918)
                     /locus_tag="PK362_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75305.1"
                     /translation="MKRLWGLMGLILAGTAGAVEGKLAPYGSVGVGYESNVFQAPSTL
                     VGVADPVRGDIFYRTVVGLDAGMKVVEGGKVFILFDHEENHFSSHSVLNNYGTEVRAG
                     YRQAWGGHWAAGLTGQWEKQNERGVDVDGVPLTQTYAYRALDISPRLEWNGEPFSATG
                     RTEATLTFTRRVADYDNPPSTPSVQAQDYTQSEIGLRLVQNYPDRYAAAFEYTLGKRD
                     YDDYLARLGGPASVEGDATTDGTRRSQKDHRLTASWERNIRPGTRWEVGAKAVLRDDG
                     YQNYFSYKQMGLYAKGKVRFESRRELSGKI"
     gene            complement(992..>1323)
                     /locus_tag="PK362_10545"
     CDS             complement(992..>1323)
                     /locus_tag="PK362_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75306.1"
                     /translation="EDREEDRREDRREDRHGGHDRGEAHDATDDRVTGGDREDHRARR
                     ERLESKLDRLETGLEDARKSGDDAKAGRLEKEIKDVKDKLAGMDDGAKKTKTESETKD
                     STRTKNR"
BASE COUNT          194 a          459 c          368 g          302 t
ORIGIN      
        1 gattttcccg ctcagttccc gccgggactc gaaccgcact ttgcctttgg cgtaaaggcc
       61 catctgtttg tagctgaaat aattttggta cccgtcgtcg cggaggaccg ccttggcccc
      121 cacttcccac cgcgtgccgg ggcggatatt ccgctcccag gacgccgtca ggcgatggtc
      181 cttttgactt cggcgggtcc cgtcggtcgt ggcatcgccc tccaccgagg cgggcccgcc
      241 caatcgggcc aggtaatcgt cgtaatcgcg ttttcccagg gtgtattcga aggcggcggc
      301 gtaccggtcc ggataattct gcaccaggcg caggccaatt tccgattgtg tgtaatcctg
      361 ggcctgaacc gacggggtcg aaggcggatt gtcgtaatcc gccacccggc gcgtgaaggt
      421 caaggtcgcc tcggtgcgtc ccgtcgccga aaaaggctcc ccgttccatt ccaaccgggg
      481 cgaaatgtcc agggcccggt aggcgtaggt ttgcgtgagc ggaacgccgt ccacgtccac
      541 gccccgttcg ttctgctttt cccactggcc cgtgaggccc gcggcccaat ggccgcccca
      601 ggcctggcgg taacccgccc gcacttccgt accgtagttg tttaataccg aatgggagga
      661 gaaatggttt tcctcgtggt caaacagaat gaaaaccttt ccgccctcca cgaccttcat
      721 tcccgcgtcc aggcccacga cggttcggta aaaaatgtcc ccccgcaccg ggtccgcgac
      781 ccccaccagg gtcgacggcg cctgaaacac gttggattcg tagcccacgc ccaccgatcc
      841 gtaaggcgcc aatttgccct ccacggcccc ggccgttccg gccaagatca accccatcaa
      901 cccccataac cttttcatat gttctcctcg ttgaatggtt tcaaacagcg gcgggggccc
      961 ccccgtcggt ggctgcgtcg tttcggaatt attagcggtt cttcgtccgg gtcgaatcct
     1021 tggtttcgga ttcggttttg gtttttttgg ccccatcgtc cattccggcc aatttatcct
     1081 tgacgtcctt gatttccttt tccaggcgtc cggctttcgc gtcgtcgccc gatttccgcg
     1141 cgtcttcgag gccggtttcc agacgatcca gcttggactc caaccgctcc cggcgcgcgc
     1201 ggtggtcttc ccggtccccc ccggtcacgc ggtcatcggt cgcgtcgtgg gcttcgcccc
     1261 gatcgtgacc cccgtgccgg tcttcccgtc ggtcctcgcg gcggtcctct tcccggtctt
     1321 cct
//
LOCUS       DAOQLS010000675         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_86463_length_1322_cov_3.559590, whole genome shotgun sequence.
ACCESSION   DAOQLS010000675 DAOQLS010000000
VERSION     DAOQLS010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86463_length_1322_cov_3.559590"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PK362_10550"
     CDS             complement(<1..395)
                     /locus_tag="PK362_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC75307.1"
                     /translation="MGRGARIRKGFTLIELMLVVAIIGLLAAIAIPKFANLVVKAKEA
                     SIKGKLGTLRSAISIYYADNEGIYPPNLVASPSPLVPKYLDEIPYIAIPTFPAHVRGN
                     LQSGELAGGRPNVADFAVPAPGNSCAWAYG"
     gene            complement(571..>1322)
                     /locus_tag="PK362_10555"
     CDS             complement(571..>1322)
                     /locus_tag="PK362_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNC75308.1"
                     /translation="VDMKELEEAIDRVIAGPERKSRMMSEKEKLVIAYHESGHTLVAK
                     KLPTSDPVHKVSIIPRGPALGYTLQLPTEDKYLTTRSEITNRLCVLLGGRMAEQLIFN
                     EMTTGAQDDLSKATQIAHKMVCEYGMSDRLGPITYRKKSEELFLGRELGEGQNYSDQT
                     AQAIDEEVKRIIADAQERVRKILSENRGALETLAKALMEKEVLNGEEITALVEGRPLP
                     TPPSTPEAGSPPRATEGGAGTGSFSPAPSPA"
BASE COUNT          249 a          352 c          407 g          314 t
ORIGIN      
        1 ccgtaggccc aggcgcagga gttgccgggg gccgggacag caaagtcggc gacgttgggt
       61 cgaccgccgg cgagctcgcc gctctgcaaa ttgccacgca cgtgggctgg gaatgtcgga
      121 atagcgatgt agggaatttc gtcaagatat ttgggaacca aagggctcgg ggaagccacc
      181 aaattggggg ggtaaatccc ttcattgtcc gcgtagtaaa tactgatggc gctccgaagg
      241 gttccgagct tgcccttgat cgaagcctct ttcgctttca ccaccaaatt ggcgaatttg
      301 ggaatggcga tggcggccag gaggccgatg atggccacca cgagcattag ctcgatcagg
      361 gtaaagcctt ttcgaattcg tgcacctctg cccatgcttt cagtttagag caaataataa
      421 gagttgtcaa tggaaaaaat taccgacagc gttaaaatca gattgtttta tagatatgaa
      481 tttaatgtgt gatttaaatt cgctgtattg gcataaagaa ccccgccaac gggattggcg
      541 gggttcttta tgatccgatg aagagatctt ctaagccggg ctgggggccg gggaaaagct
      601 cccggtgccg gcgccgccct ccgtggcccg gggcggagat ccggcttcgg gggtcgaggg
      661 gggcgtgggc aaaggacggc cctccaccag ggcggtgatt tcctcgccgt ttaacacctc
      721 tttttccatc aaggctttgg ccagagtttc cagcgccccg cggttctccg aaaggatctt
      781 gcgcacgcgt tcttgggcgt cggcaataat ccgtttgact tcttcatcga tggcctgggc
      841 cgtctgatcg gagtagtttt gtccctcgcc cagttcccgg cccaggaaca gctcctcgga
      901 ctttttgcgg taggtgatgg ggcccaggcg gtccgacatg ccgtattcgc acaccatttt
      961 gtgggcgatt tgcgtggcct tggacaagtc gtcctgggcg ccggtggtca tctcgttaaa
     1021 gatcagctgc tcggccatgc gtccgcccaa cagcacgcag aggcggttgg tgatctccga
     1081 tcgggtggtc aaatacttgt cttccgtcgg caattgaagg gtgtagccca gggccggtcc
     1141 tcgggggatg atggacactt tgtgcacggg gtcggaggtc ggcagttttt tggccaccag
     1201 cgtgtgtccc gactcgtggt aggcgatgac gagcttttcc ttctcgctca tcatgcggct
     1261 tttgcgttcc ggtccggcga tcacccggtc gatggcttcc tccaactcct tcatgtccac
     1321 cc
//
LOCUS       DAOQLS010000676         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_86565_length_1321_cov_7.272512, whole genome shotgun sequence.
ACCESSION   DAOQLS010000676 DAOQLS010000000
VERSION     DAOQLS010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86565_length_1321_cov_7.272512"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..777
                     /locus_tag="PK362_10560"
     CDS             319..777
                     /locus_tag="PK362_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNC75309.1"
                     /translation="MIPEALNKKGFTLIELMLVVAIIGLLAAIAIPKFANLVVKAKEA
                     AIKGQLGTIRSALSIYYSDNEGIHPSTNYGALRTSLTTGGKYLDEIETFSIPTVPGHR
                     GHPNFNPGSVNDVFFGNAASGGEWAYLLTSRTIVVNCTHTDSKGTTWSLW"
     gene            complement(826..>1321)
                     /locus_tag="PK362_10565"
     CDS             complement(826..>1321)
                     /locus_tag="PK362_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNC75310.1"
                     /translation="NEGALRAGRGQKSSIGIRVDLRAEQKANPFVGTSYEHRTFFSRL
                     QHFVLLSDAFLVVTGGIGTTLEAMMIWQLLQVRKIYNTPLVFVGPQWADLVDWARRHM
                     LRAKPPLADPRDLVIPHCVNTVEEATEIVRRHREKWANNGRRQTPERRRNGKNLPRKD
                     RHAW"
BASE COUNT          280 a          384 c          368 g          289 t
ORIGIN      
        1 tcgaaccctc ggtacgggtt taccccgtac agcggattag caatccgccg ccttcagcca
       61 gctcggccac ctttccatgg gggtttcggc ccctctgggg ccgcccatgg ccccaaggct
      121 gtccctttcc acaccctccg acaaacgggt gccccaccga tcggggaggc aaaaaggggc
      181 ggcagctatc gccgctgtgc tcttggggcc agataaattg gttccgtcca acaacggagc
      241 gtattttacc ttttccctag gcccaatgac gccttgaaat tcctaccggc aagcctatac
      301 tgaagcaagg acaaaattat gattcccgag gcgttaaata aaaagggatt taccttgatt
      361 gaactgatgc tcgtggtggc gatcatcggc ctcttagcgg ctatcgccat ccccaagttc
      421 gccaacctgg tggtcaaagc caaggaagcg gcgatcaaag gacaattggg cacgattcga
      481 agcgcgctat ctatctacta cagcgataac gaaggaatac atccctccac aaactacggc
      541 gcgctaagga cttcattgac caccggtgga aaatatctgg atgagattga gactttctca
      601 attcccaccg tgccggggca tcggggccac cccaatttca atccgggcag tgttaatgac
      661 gtattttttg gaaatgccgc aagtggcggc gagtgggctt atctcctcac cagccggacc
      721 attgtcgtca attgcactca taccgactcg aaagggacga cctggagttt gtggtaaaaa
      781 agtttttcct tgtgctcgtc cgttccacgg agcctcctca aatatttacc aggcatgccg
      841 gtcttttctt gggaggttct tcccgtttcg tcggcgctcg ggggtctgac gccggccgtt
      901 attggcccat ttttcccgat ggcggcggac gatctccgtg gcttcttcga cggtgttcac
      961 gcaatgggga atgacgagat cccgggggtc cgccaggggc ggtttcgcgc ggagcatgtg
     1021 ccgacgggcc caatccacaa ggtccgccca ttgggggccc acgaaaacga ggggcgtgtt
     1081 gtaaattttc cgcacctgaa gcagttgcca aatcatcatg gcttccaacg tcgttccgat
     1141 gcccccggtc acgacgagga aggcgtcgga caggagaacg aagtgctgca aacgggagaa
     1201 aaaagtccgg tgctcgtagg aggttccgac gaaagggttt gctttctgtt ccgcccgaag
     1261 gtccacccgg atgccgatgg acgatttctg cccccgcccc gcgcggaggg ccccctcgtt
     1321 g
//
LOCUS       DAOQLS010000677         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_86740_length_1320_cov_2.886166, whole genome shotgun sequence.
ACCESSION   DAOQLS010000677 DAOQLS010000000
VERSION     DAOQLS010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86740_length_1320_cov_2.886166"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..>1320
                     /locus_tag="PK362_10570"
     CDS             188..>1320
                     /locus_tag="PK362_10570"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNC75311.1"
                     /translation="MSRDQRMAANWALLHARARAAERGVPLAVVFCLTPGFPGATLRA
                     YDFMLRGLEALEARLAAAKIPFFLLAGEPTETLAAFVRKEKIGAVVTDFDPLKIKRRW
                     KTKLAEALPVAFTEVDAHNVVPAWLASPKLEYGAYTLRPKIHKLLDEFLTDFPALQPQ
                     TTPWPEIVKRVDWNRVREGLKVDTSVGPVDSWTPGEAGGARTLGLFLSKKLDAYHGRR
                     NDPTLDGQSDLSPYLHFGQVSAQKLSWEVRRRALDTPSGDAFLEELIVRRELSDNFCL
                     YNEKYDSFEGFPAWAQKSLNAHRRDKRDFLYSGKQFENAATHDELWNAAQREMVHRGK
                     MHGYMRMYWAKKILEWSTSPEEAMATAIDLNDRYELDGRDPNG"
BASE COUNT          259 a          441 c          396 g          224 t
ORIGIN      
        1 aacctctggg gcgcctgggc ctgcccctgg tccgccggga actcgaaagg atttttcggt
       61 tccgtcagga agccgtcgcc cgccacttcg gcgtgaggcc cgtcccatcc taaaacccct
      121 gcccacgacg cgccgaagcc ggtcgctcaa ccagcgcccg ccggggccgg gccccgtcgt
      181 ctattggatg agccgcgatc agcgcatggc ggccaattgg gccctattgc acgcccgggc
      241 ccgggccgcc gaacggggcg tccccttggc ggtggttttt tgcctgacgc ccggtttccc
      301 cggggccacg cttcgggcct acgatttcat gctccggggc ctggaagccc tggaggcccg
      361 cctggcggcg gccaaaatcc ccttctttct tttagcgggc gagccgacgg aaacgctggc
      421 ggcctttgtg cgaaaagaaa aaatcggggc ggtggtcacc gattttgatc ccctaaaaat
      481 caagcgccga tggaagacga aattggccga ggccctgccc gtggccttca ccgaggtcga
      541 cgcccacaac gtcgtgcccg cctggctcgc ctcgcccaaa ctcgaatacg gcgcctacac
      601 cctgcggccg aaaatccaca aactcctgga cgaattcctg acggacttcc ccgccctcca
      661 accccaaaca acgccctggc ccgaaatcgt caaacgcgtg gattggaatc gggttcggga
      721 gggattgaaa gtcgacacgt cggtggggcc cgtcgattcc tggacgcccg gggaagccgg
      781 cggggcccgg accctcggcc tcttcctttc caaaaagctg gacgcctacc acggacggcg
      841 gaacgacccg accctggacg gccaatcgga tctgtcgccc tacctgcact tcgggcaggt
      901 gtcggcccag aaattgtcct gggaagttcg tcggcgggcc ctggacacgc cttcgggcga
      961 cgcttttctt gaggaactca tcgtacggcg ggaactgtcg gacaactttt gcctctacaa
     1021 cgaaaaatac gattctttcg aggggttccc cgcctgggcc caaaaatccc tcaacgccca
     1081 ccggcgggac aaacgggact ttctctattc agggaagcaa ttcgaaaacg ccgccaccca
     1141 cgacgaactc tggaacgcgg cccagcggga aatggtccac cgggggaaaa tgcacgggta
     1201 tatgcggatg tactgggcga aaaaaatatt ggagtggtcg acgtctcccg aggaggcgat
     1261 ggccaccgcg attgacctca acgaccggta cgaactggac ggccgggacc ccaacggcta
//
LOCUS       DAOQLS010000678         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87016_length_1318_cov_2.640538, whole genome shotgun sequence.
ACCESSION   DAOQLS010000678 DAOQLS010000000
VERSION     DAOQLS010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87016_length_1318_cov_2.640538"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK362_10575"
     CDS             <1..212
                     /locus_tag="PK362_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75312.1"
                     /translation="HANGANMDGLETRETRRLLPGTLFSIEPGIYLRDFGVRSEINVG
                     LPGGRAVVTGATQRNSWAILGENPA"
     gene            209..568
                     /locus_tag="PK362_10580"
     CDS             209..568
                     /locus_tag="PK362_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75313.1"
                     /translation="MKCGVFFEKARGRWPMTMWVEDVDFEVLGKGFPVWWVATNGFTF
                     YFSGLKELDECVRVLGQKLLERPGVLAKQGTSLSWTSNKHWLSRLPAKVKTWKFRQKA
                     VADLKRARGEFNQKEAF"
     gene            647..>1318
                     /locus_tag="PK362_10585"
     CDS             647..>1318
                     /locus_tag="PK362_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC75314.1"
                     /translation="MKKILALVLSFLALPLYAHPVDPRPDKPLVQLALLLDTSNSMDG
                     LIDQAKSQLWRIVNDLAGARCRGYAPRIEVALYEYGNSGLPAGERYLRQVVSFTSDLD
                     RVSEKLFGLRTNGGEEYCGAVINDAVKNLAWDTRSSTYKTLFIAGNEPFTQGGVSYRD
                     AIQRAVGRDIVVNTIFCGDRREGIETAWANGAERGRGAYLTINQDQQIAVMRSPYDDE
                     IERLGR"
BASE COUNT          281 a          368 c          413 g          256 t
ORIGIN      
        1 accacgccaa cggcgccaac atggacgggc tggagacccg ggaaacccgg cgcctgctcc
       61 cgggcaccct cttttccatt gagccgggga tttatttaag ggatttcggt gtgcggagcg
      121 agatcaacgt gggtctcccc ggggggcggg cggtggtgac gggcgcaacg caaaggaatt
      181 cctgggcgat tctcggggag aacccggcat gaaatgcggc gttttttttg aaaaggcgcg
      241 ggggcgctgg cccatgacga tgtgggtgga agacgtggat ttcgaggtgt tgggaaaagg
      301 gttccccgtt tggtgggtgg ccaccaacgg attcactttt tatttcagtg gcctaaagga
      361 attggacgaa tgcgttcggg tgttgggtca aaaattattg gaacgccccg gcgttttggc
      421 caaacagggg acgagcctct cctggaccag caacaagcat tggctgagcc gccttccggc
      481 aaaagttaaa acatggaaat tcaggcaaaa ggcggtggcg gatttaaaac gggcgagggg
      541 ggaattcaac caaaaagaag ctttttagaa gaatcgaaga attcgcccgg gaacttttgc
      601 ggtcctccgg tgtcgtttgg ggcaggaacc atcccaggag gctgtcatga aaaagattct
      661 cgccctcgtt ttaagtttct tggccctgcc cctttacgcc catcccgtcg atccccgtcc
      721 cgataagccc ttggtccaat tggcgcttct gttggacacc tccaacagca tggacggatt
      781 gatcgaccaa gccaaatccc aactctggcg aatcgtcaac gacctcgccg gggcccggtg
      841 ccggggttac gccccccgga tcgaagtggc cctctacgaa tacgggaact ccggtcttcc
      901 ggccggggaa cggtatcttc ggcaagtcgt gtccttcacc agcgatttgg accgcgtttc
      961 ggaaaagctc ttcgggcttc gaaccaacgg cggcgaggag tactgcggcg cggtcattaa
     1021 cgacgccgtc aaaaatctgg cctgggacac ccgatcctcc acgtacaaga cgctcttcat
     1081 cgcgggcaac gaacccttca cccagggcgg tgtgagctac cgggacgcca tccaacgggc
     1141 cgtgggccgg gacatcgtgg tcaacaccat cttttgcggc gaccgccggg aagggattga
     1201 aaccgcctgg gccaacggcg cggagcgggg ccggggcgcc tacttgacca tcaaccagga
     1261 ccagcaaatc gccgtgatgc gcagccccta cgacgatgaa attgagcggt tgggccgg
//
LOCUS       DAOQLS010000679         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87234_length_1316_cov_3.848533, whole genome shotgun sequence.
ACCESSION   DAOQLS010000679 DAOQLS010000000
VERSION     DAOQLS010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87234_length_1316_cov_3.848533"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK362_10590"
     CDS             <1..241
                     /locus_tag="PK362_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HNC75315.1"
                     /translation="MMLETDVIDGLLQLCENATQEWPKKVFFMGQLAFEGETFWTRFL
                     HNRTSFVLQRRLLFNGLQAVILPIRVRLKRAVLPQ"
     gene            256..>1316
                     /locus_tag="PK362_10595"
     CDS             256..>1316
                     /locus_tag="PK362_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75316.1"
                     /translation="MSDAPDIVQLSAKKFLGACALLALAIVLSGRPLPPWVLGAEVLA
                     TIIALFVTGSIRYRLDKNALTYGAGLVVAASFWTLWNPGVRAAAMAQDRTLGTALWDV
                     FQRHFLTFAGLDDLIHLDTMLFILGLTFFVAVIAQTRLLETASFGVLAKTKGKLVPTI
                     AALTAMVSVASGVLDGVSMIGLMIRTLVILLFMAKIKDESVIYAVMVSTVVTTVCGMW
                     LAYGEPPNLIMKSNLHPHLDNAFFLRYCLPAAVGSYLIVFWNVRKRLAGKTVDLARLD
                     ILDQHIADVRFLQVTRHGETLTPVEFAEAHRAELGSHADAVIKRLHQGVPLGEALVNE
                     HLPREKRLPLLDKFMDSSLA"
BASE COUNT          221 a          436 c          371 g          288 t
ORIGIN      
        1 gatgatgctg gaaaccgacg tgatcgacgg cctccttcaa ctctgcgaga acgccaccca
       61 ggaatggcct aaaaaagtgt ttttcatggg ccaattggcc ttcgaaggcg aaacgttttg
      121 gacgcggttc cttcacaacc ggacctcttt cgtgctccag cggcggttgc tgttcaacgg
      181 ccttcaggcc gtcatccttc ccattcgagt ccggctcaag cgggccgtgc tcccccaata
      241 aatccggaga ttgccatgtc cgacgcgccc gacatcgtcc aactgtccgc caaaaaattc
      301 ctgggggcct gcgcccttct ggccctggcc atcgtccttt ccggacgtcc cctgccccct
      361 tgggtgttgg gggccgaagt tttggccacc atcatcgccc tctttgtgac ggggtccatc
      421 cgttatcggc tggacaaaaa cgccctgacc tacggggcgg gactcgtggt ggccgccagt
      481 ttttggaccc tgtggaaccc cggcgtccgc gccgcggcca tggcccagga tcggaccctc
      541 ggcacggcgc tttgggacgt tttccaacgt cactttttga cgttcgcggg actggacgac
      601 ctgatccatt tggacaccat gctcttcatc ctgggcttga ccttctttgt ggcggtcatc
      661 gcccagaccc ggttgctcga aaccgccagt ttcggcgttc tggccaaaac caagggcaaa
      721 ctggtcccca ccatcgcggc gttgacggcc atggtgtccg tggcctcggg cgtcctggac
      781 ggcgtttcca tgatcggcct catgatccgc acgctggtca tcctcctctt tatggccaag
      841 atcaaggacg agtcggtcat ttacgccgtc atggtttcca cggtcgtgac gacggtctgc
      901 ggcatgtggc tggcctacgg ggagcccccc aacctcatca tgaagtccaa cctccacccg
      961 catttggaca acgccttttt tctgcgctat tgcctaccgg cggcggtcgg ctcctacctc
     1021 atcgtttttt ggaacgtccg gaaacgcctg gcgggcaaaa ccgtcgattt ggcccgcctg
     1081 gacattttgg accagcacat cgccgacgtg cgtttcctgc aggtgacccg ccacggggaa
     1141 accctgaccc cggtggagtt cgccgaggcc caccgggcgg aactgggttc ccacgcggac
     1201 gccgtgatca agcgcctcca ccagggcgtt ccgctgggcg aagccctggt gaacgagcac
     1261 ttgccacggg aaaagcggct ccccctcctg gataaattca tggattcgtc cttggc
//
LOCUS       DAOQLS010000680         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87237_length_1316_cov_3.794607, whole genome shotgun sequence.
ACCESSION   DAOQLS010000680 DAOQLS010000000
VERSION     DAOQLS010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87237_length_1316_cov_3.794607"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PK362_10600"
     CDS             <1..795
                     /locus_tag="PK362_10600"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNC75317.1"
                     /translation="KDTNGIELVKSLKVMRPELVDLWAYFEAGFHNLAVASVRQRYPK
                     EAIKTALGLMGQGQMSLTKCVVLVDPSVNVRNFAEVLDALRNHFNPADDWMLLPGTPQ
                     DTLDFTSFKMNLGSKMILDATSLGTRRPPPRSERPDRVNVDGLDLPSRNWRDALLAVR
                     TPGPGRPVIEKILRNPAFSGYNLIAAVSDDVPLDDDELLLWGLFTRFDCARDVVPASV
                     TLNGAWPVYGGPLGIDATWKAGYPKPLTMTESIVRRVDERWKEYGL"
     gene            792..>1316
                     /locus_tag="PK362_10605"
     CDS             792..>1316
                     /locus_tag="PK362_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNC75318.1"
                     /translation="MIFEDKNLPVLWEKVRSGERLSEEDGRVLYASPDLLGVGWMADW
                     VRRARHGDRATFVFNRQINPTNICVLSCKFCDYASKEGWDNAYVLSEKEILDRLSPEL
                     REVHIVGGLYRKWSFDDYLNVLRVIRRAYPQIQLKAYTAVEIDFFARRENLSIAQVLQ
                     RLKDEGLVCLPGGGA"
BASE COUNT          254 a          395 c          401 g          266 t
ORIGIN      
        1 aaggacacga acggcatcga gctggtgaaa tcattgaagg tcatgcggcc ggagctcgtg
       61 gacctctggg cttatttcga agccgggttt cacaatttgg cggtggcctc ggtccgtcaa
      121 agatacccca aagaggcgat caaaaccgcc ctggggctca tgggccaagg gcagatgtcc
      181 ttgacgaaat gcgtcgtcct ggtggatcca tcggtcaatg tgcggaattt cgcggaggtg
      241 ttggacgccc tacgaaacca cttcaacccc gccgacgact ggatgctttt accggggacc
      301 ccccaggaca ccctggactt tacgagcttc aaaatgaacc tgggtagcaa gatgatcctg
      361 gacgccacgt ccctggggac ccgacgcccc cccccgcggt cggaacgccc ggaccgcgtc
      421 aacgtcgacg ggttggacct tccgtcccgc aattggcgcg acgccttgtt ggcggtccgc
      481 accccgggtc cgggacggcc ggtcatcgaa aaaattcttc gaaatccggc cttttcgggg
      541 tacaatttga ttgccgcggt gagcgacgac gtgcccttgg acgacgacga gttgcttctg
      601 tggggccttt tcacccggtt tgactgcgcc cgggacgtcg ttcccgcgtc cgtgaccctg
      661 aacggcgcct ggccggtcta cggcgggccc ttgggcatcg acgccacctg gaaagcgggg
      721 tatcccaagc ccctcacaat gacggagtcg atcgtacggc gcgtcgacga acgttggaag
      781 gaatacggat tatgattttc gaagacaaaa acctcccggt cctttgggaa aaagtccggt
      841 ccggcgaaag gctgtcggag gaagacggcc gggtcctcta cgccagcccg gacctcctgg
      901 gggtggggtg gatggcggat tgggtccgcc gggcccgcca cggcgatcgg gccactttcg
      961 tgtttaatcg ccagatcaat ccgacgaaca tttgcgtgct ttcctgtaaa ttttgcgact
     1021 acgcctccaa ggaaggttgg gacaacgcct acgtcctttc cgagaaggaa attctggacc
     1081 gcctttcgcc ggagctgcgg gaagttcaca tcgtgggcgg cctttaccgc aaatggtcct
     1141 tcgacgatta tctcaatgtt ctgcgggtca ttcgtcgagc ctacccccag atccagctga
     1201 aggcctacac ggccgtcgaa atcgattttt tcgcccgtcg ggaaaacctt tccatcgccc
     1261 aggtcctcca gcgactgaag gacgaaggat tggtctgcct tccggggggc ggggcc
//
LOCUS       DAOQLS010000681         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87567_length_1314_cov_2.573471, whole genome shotgun sequence.
ACCESSION   DAOQLS010000681 DAOQLS010000000
VERSION     DAOQLS010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87567_length_1314_cov_2.573471"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK362_10610"
     CDS             <1..462
                     /locus_tag="PK362_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNC75319.1"
                     /translation="PFEAAMAAQHALTQRFSAEFSIRPFLIRDQDRTLARLLEWTRDP
                     SPHVRRLCSEGTRPRLPWATRIPAFVKNPEPVFPILEILKNDPSLYVRRSVANHLGDV
                     AKDHPEKAFALCERWLTGASSELKWLIRHALRHPAKKGDKNALRLRSAAKA"
     gene            673..>1314
                     /locus_tag="PK362_10615"
     CDS             673..>1314
                     /locus_tag="PK362_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC75320.1"
                     /translation="MPKVLLTSVCRPFGEKEGDAASVGYELLHKQVTRAQGIFSPRAL
                     HVHFSLDYIAANLDAPTVVLQYPSKRELIRELKKGYDYVGISFLMAVFHKMKDAVALV
                     REHAPQSRIVLGGYGTILTDDDLKPYADFICREEGVAFFRRLLGEPERPKPYRHPHLV
                     SDLKIFSVPVSKTAKIFAGLGCPNGCDFCCTSHFFKRRHVKLLPTGKDIFDQVE"
BASE COUNT          255 a          451 c          360 g          248 t
ORIGIN      
        1 ccctttgaag ccgccatggc ggcccaacac gccctgaccc agcggttcag cgccgaattt
       61 tccatccgcc ctttcctgat ccgcgatcaa gaccgcacgt tggctcggct cttggaatgg
      121 acccgggacc ccagccccca cgtccgccgg ttgtgctccg aagggacccg cccccgcctg
      181 ccctgggcga cccgaattcc ggctttcgtc aaaaaccccg agcccgtgtt cccgattttg
      241 gaaattttga aaaacgaccc cagcctctac gttcgacgga gcgtggccaa tcacctgggg
      301 gacgtggcca aggaccatcc ggaaaaggcc tttgccctct gcgagcgttg gctgacgggg
      361 gcgtcgagcg agctgaagtg gctgattcgc cacgccctcc ggcatcccgc caaaaagggg
      421 gataaaaacg cccttcgact gaggtccgcc gccaaggcat gagttccggg gccgcgaggc
      481 gcccgaagcc cgtcaaaagg agccgcgccg tatgaccgcg gtcatatcgg ctccgcccgc
      541 ccttcaacta tagtcccctc cacaatatgg cgcccaggcg gtcgcttcga aacgattgat
      601 tctgttccgt cggccttcgc ccgttggacc cccatccgtt ccaaccgcac accccacgtc
      661 aaaggaaaaa caatgcccaa agttctctta accagcgtct gccgtccctt cggggaaaaa
      721 gaaggcgacg ccgcttccgt cggttatgaa ttgctccaca agcaggtcac ccgcgcccag
      781 gggatcttca gcccccgggc cctgcacgtc catttttccc tggattacat cgccgccaac
      841 ctggacgccc ccacggtggt gctccaatac ccttcgaaac gcgagttgat ccgcgagttg
      901 aaaaagggct acgactatgt cggcatctcc tttttgatgg ccgtgttcca caaaatgaag
      961 gacgcggtgg ctctggtgcg ggagcacgcg ccccaaagcc ggatcgtcct gggcggctac
     1021 ggcaccatat tgacggacga cgacctgaag ccctacgccg actttatttg ccgggaggag
     1081 ggcgtggcct ttttccgccg gttgttgggc gaacccgaac ggcccaaacc ctaccgccac
     1141 ccgcacctgg tgagcgacct gaaaatcttc tcggtgcccg tgtccaagac ggcgaaaatt
     1201 ttcgccggac tggggtgccc caacggttgt gacttttgct gcacctccca cttctttaag
     1261 cgccgccacg tgaagctgct tccgaccggc aaggacattt tcgaccaggt ggag
//
LOCUS       DAOQLS010000682         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87617_length_1313_cov_4.889507, whole genome shotgun sequence.
ACCESSION   DAOQLS010000682 DAOQLS010000000
VERSION     DAOQLS010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87617_length_1313_cov_4.889507"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PK362_10620"
     CDS             complement(<1..976)
                     /locus_tag="PK362_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC75321.1"
                     /translation="MKKVLLSGIPLLLGGFLRAEPLSLEHFVRGVLDRHPGLSAADAG
                     RRVAQAQLAEARAQRLPRLTAFSQLTRGDGPVYAFASLLDRRSFTASDFAVDALNRPG
                     YATHLRSSLALGVPLFAGFAIQSQERLAALGVEKAAWEEEQARQSLRRAVLEGALQQM
                     LARQMESSLAARIEAAEEEIASAQKLRAKGLVLGSDYFAAEAVLSSLRAQRLEARERR
                     AGAGETLALLRGDAVSPVEIAGSLEASPPSLPSEEAMRRALADRPDLAALDRGALQAA
                     TAARQAGRFPWPTVDALAEAQSNTDDFSSNAGQRLLMLRAQWALGDPAR"
     gene            complement(973..1173)
                     /locus_tag="PK362_10625"
     CDS             complement(973..1173)
                     /locus_tag="PK362_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNC75322.1"
                     /translation="MNIERVLRGIAGFFILVSLGLAHWHSAHWLWFTAFVGLNLFQSS
                     FTNWCPMMTLLRKCGGRPSETP"
BASE COUNT          258 a          428 c          424 g          203 t
ORIGIN      
        1 cccgggcggg gtccccgaga gcccattgag cccgaagcat tagcaggcgt tgtccggcgt
       61 tgcttgaaaa atcatcggtg ttggactgcg cttccgccag ggcgtcgacc gtgggccagg
      121 ggaaccttcc cgcctgtcgg gccgccgtcg cggcttgaag ggcgccgcgg tccagggcgg
      181 ccaaatccgg gcggtcggcc agggcccggc gcatggcctc ttcggacggc agggagggcg
      241 gcgaggcctc cagggatcca gcgatttcca ccggcgagac ggcgtcgccc cggagaaggg
      301 ccaacgtttc cccggccccc gcgcgtcttt cccgggcctc caaacgttga gcccgcaggg
      361 aggagaggac cgcttccgcg gcgaaataat ccgaccccaa caccaatccc ttggcccgca
      421 atttttgcgc gctggcgatt tcctcctcgg cggcttcgat gcgggcggcc agcgaacttt
      481 ccatttgacg ggccagcatt tgctgaagcg ccccttccaa caccgcccgc cgaagagact
      541 gacgggcctg ctcctcctcc caggccgctt tctcgacgcc cagggccgca agccgctcct
      601 ggctttgaat ggcaaacccc gcgaacaacg ggacccccag ggccagggaa ctgcgcaggt
      661 gcgtggcgta ccccggccgg ttcagggcgt cgacggcgaa atcggacgcc gtaaaagaac
      721 ggcgatccag gagggaggca aaggcgtaga ccggtccgtc tccccgggtc aactggctga
      781 aagcggtgag tcggggaagc cgctgagccc gggcttccgc tagttgcgcc tgggcgacgc
      841 ggcgcccggc gtcggcggcg gacaatccgg gatggcggtc caaaacaccc cggacaaaat
      901 gttccagaga caggggctcg gcccgaagaa agccccccag gaggagcgga attccggaaa
      961 gaaggacttt tttcacgggg tttcgctggg acgaccgccg catttacgaa gaagcgtcat
     1021 catgggacac cagttggtga aagacgactg gaacaggttc aaccccacaa aggccgtgaa
     1081 ccacaaccag tgggcgctgt gccaatgggc cagacccagg ctcaccaata tgaaaaaacc
     1141 ggcaatcccc cgcaaaacac gctcaatgtt catgaattcc tcctatacct tttggatgcg
     1201 gaaaggctca aaaggattcg cccccgggtc cacccggagg ttattttggc aattcgatgg
     1261 gagagcggcc atgatgcggg tcatcgtccg cgttgagagc cccgccggtc ccc
//
LOCUS       DAOQLS010000683         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87762_length_1312_cov_6.125696, whole genome shotgun sequence.
ACCESSION   DAOQLS010000683 DAOQLS010000000
VERSION     DAOQLS010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87762_length_1312_cov_6.125696"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1154)
                     /gene="mdh"
                     /locus_tag="PK362_10630"
     CDS             complement(204..1154)
                     /gene="mdh"
                     /locus_tag="PK362_10630"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNC75323.1"
                     /translation="MRRKISVIGAGNVGATLAQRLAETELGDVVMVDIPQTEGMPAGK
                     ALDILQSGPVYGYDSRVTGATDYAPTAGSDVVVITAGIPRKPGMSRDDLLNTNAGIVK
                     AVSAQVAAHSPEAILIVVSNPLDAMAHVALRTTGFPKNRVIGMAGVLDSARMATFLAE
                     ALSVSVEDIQPTVLGGHGDTMVPLARYTTVAGVPVTELLPKDKLDAILQRTRDGGAEI
                     VKLLKTGSAYYAPSASVVAMVKAILLDKKRILPCAAYLEGEYGARGLFLGVPVKLGAR
                     GVEQIISLSLTADEKTALQKSIGAVQELVDVLKKQNIVTS"
BASE COUNT          249 a          416 c          363 g          284 t
ORIGIN      
        1 tccagggctt cccggaaaga ctggtaatga tcctgaaaaa ccatcacttt ggacattcgg
       61 ccgaaactgc gttccgtgat catgaggaac cgatggccgt tcttggtgat ttgacactcc
      121 aaggtgtatt cccgtttccc gcactggatg gtttccgtgc tcatgcccat tctccccggg
      181 cgcgccccgt ggggcgccgc cgtctacgaa gttacgatgt tctgtttttt taaaacgtcc
      241 accaattcct ggacggcgcc gatggatttt tgaagcgcgg tcttttcgtc cgccgtcaag
      301 gaaagggaaa tgatttgctc cacgccccgg gcgcccaact tgacgggaac gcccaggaaa
      361 agtccccggg cgccgtattc gccttccaaa taggcggcgc agggcagaat ccgtttcttg
      421 tccaacagga tggctttgac catggccacg acgctggccg acggggcgta ataggcggat
      481 cccgtcttga gcaatttcac gatttcggcc ccgccgtccc gggtgcgttg aagaatggcg
      541 tccaatttat ccttcggcaa caattccgta acgggaaccc ccgccacggt cgtgtagcgg
      601 gccaagggca ccatggtgtc cccgtggccg cccagcaccg tcggttgaat gtcctccacc
      661 gacacggaca gggcttctgc caagaaagtc gccatgcgcg ccgaatcgag aaccccggcc
      721 atgccgatga cccggttctt gggaaaaccc gtggtccgca gggccacgtg agccatggcg
      781 tccagggggt tcgacacgac gatcaaaatg gcttcgggcg aatgcgcggc cacctgggcg
      841 gacacggctt tcacgatgcc ggcgttcgtg ttcaataaat cgtcgcggct catgcccggc
      901 tttcgcggaa tgcccgccgt gatcaccacg acgtcggaac cggcggtcgg cgcgtaatcc
      961 gtcgcgccgg tgacccggct gtcgtacccg tacaccgggc ccgactgcaa aatgtccagg
     1021 gcttttcccg ccggcatgcc ttcggtttgc gggatgtcga ccatcacgac atcgcccaac
     1081 tccgtttccg ccagccgctg agccaacgtg gccccgacgt tccccgctcc gataacgctg
     1141 attttccgcc tcatgatgcc ctccctcgat ttcccgcgaa ccgcgttgaa taggaataac
     1201 aatactaata tttaaacttt ttttcaattc gtcatgtctt tactgtttgt ttcctttgac
     1261 tttcggaaat ccggtgaaca agggagccca cgggggcacg tcgaccgccc cc
//
LOCUS       DAOQLS010000684         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87796_length_1312_cov_3.640414, whole genome shotgun sequence.
ACCESSION   DAOQLS010000684 DAOQLS010000000
VERSION     DAOQLS010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87796_length_1312_cov_3.640414"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1312
                     /locus_tag="PK362_10635"
     CDS             <1..>1312
                     /locus_tag="PK362_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75324.1"
                     /translation="ETFQKWIDDLRGAGKKPAAGAAAPRFQDFLYKTSPGVEAQRILN
                     DDAVRALLIGIPRNSLPAFYDLLKKDAFLNATNLPPAFAPVQARIWQVFAEGPVRFGS
                     KERDAAPRSPVMDEVQASLLRSAEQNARNPRYSITSRLDTPNNSLFEAQRILADPALL
                     EMLNKLTPEQIDAVATAPGNGAFIGSPRRQLYGTALGEIIDRVSYLFDNGPFARGGLD
                     ARQRLEVLQLVEKKLAEAAKTAAGARPKAGAVSLLGGEAAAGAAPFHDHLLRTSPDIE
                     THRVLTSNKSLELANALSIQEVETLAALAREGKFLSTRKFNGTALGDFQANLLAGFLR
                     GPLSLTRPDGTQGLTETGRGVLRQAEADLAAAADKVLRMPRYHLGSRLYDDSPFTVDA
                     QRVLADGRLQDLLNGMDAVAVDLLAEAKGDGLLLTDPRTAATSLG"
BASE COUNT          232 a          427 c          408 g          245 t
ORIGIN      
        1 gggagacctt tcagaaatgg atcgacgacc ttcgcggcgc cggaaaaaaa ccggccgccg
       61 gcgccgccgc cccccgattt caagatttcc tttataagac gtccccgggc gtcgaagccc
      121 agcggatttt aaacgacgac gcggttcggg cgcttttgat cggcatcccc cggaattcgt
      181 tgccggcctt ttacgacctt cttaaaaagg acgcgtttct gaacgcgacg aaccttcccc
      241 cggccttcgc gccggttcaa gcccggattt ggcaggtgtt tgcggaagga cccgtccggt
      301 tcggttccaa agaacgggac gccgcgcccc gttctccggt catggacgaa gttcaggcca
      361 gccttctgcg gtccgccgaa cagaacgccc ggaatccgcg ttattcgatc acttcccgtt
      421 tggacacgcc caacaactcc cttttcgaag cccaacggat tttggccgat ccggccctgc
      481 tggaaatgtt gaacaaactg acgccggaac agattgacgc ggtggcgacg gcgcccggga
      541 acggggcgtt catcggttcc ccgcggcgcc aactctacgg cacggcgctg ggagagatca
      601 tcgaccgcgt gtcgtatttg ttcgacaacg gtcccttcgc ccgcggcggt ctcgacgccc
      661 ggcagcgact cgaggtcctg caactggtgg agaagaaatt ggccgaggcg gccaagaccg
      721 ccgccggggc ccgacccaag gccggcgccg tgtctttgct cggcggggaa gcggccgccg
      781 gggccgctcc gtttcacgat cacttgcttc ggaccagtcc ggacatcgaa acccatcggg
      841 tcctgacgtc caacaagtcc ctggaattgg ccaacgccct ctcgatccag gaggtcgaga
      901 ccctcgcggc cttggcccgg gagggaaaat tcctctccac tcgaaaattc aacggaacgg
      961 ccctcgggga tttccaggcg aacctcttgg cgggattcct tcgcgggcct ctgtcgctga
     1021 cgcgtccgga cggcactcaa ggcctcaccg aaaccggacg gggggttttg cgtcaggccg
     1081 aggcggacct cgcggccgcg gcggacaaag tgcttcgcat gccccggtat catttgggaa
     1141 gccgtctgta cgacgattcc ccgttcaccg tcgacgccca gcgggttttg gcggacggac
     1201 gccttcagga tttgttgaac gggatggacg cggtcgccgt cgatttgttg gccgaggcga
     1261 agggcgatgg ccttttgttg acggatccgc ggaccgccgc gacctccctg gg
//
LOCUS       DAOQLS010000685         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87847_length_1312_cov_2.494033, whole genome shotgun sequence.
ACCESSION   DAOQLS010000685 DAOQLS010000000
VERSION     DAOQLS010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87847_length_1312_cov_2.494033"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK362_10640"
     CDS             <1..430
                     /locus_tag="PK362_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75325.1"
                     /translation="PVPAIAARARRACAGYPEIDILEKDLRWMVDRGARFDYVTASLF
                     LHHVSLRERADVLAALDRLARRGLILSDLRRSLPGYWAVGALAALRRDRVAFHDGPLS
                     VRRAFRVADLENLAATAGLPHLRARSEPWFRVSLLGEKNG"
     gene            423..>1312
                     /locus_tag="PK362_10645"
     CDS             423..>1312
                     /locus_tag="PK362_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC75326.1"
                     /translation="MAERAVVVGGGPAGASAALALLAARFEVTVFERRPRFAPRVCGT
                     FLDAEALRHLTTLGVRDLIDRESVPVRRVRVTVPPRRLRVVDLPEAGAALPRPRLEAL
                     LFDEIRRRGGAVVPGVRVFPGEDSRTVRLDGPGLGEGGTPVRRADLVVWATGRPAPDP
                     AGGGPGHYGWNADFDGVDQRPGDMSLHFTPRGYVGVVTHGDGTTNVCGLYRRENVPPD
                     WENVFRESRNDSPAFARETAAARRRTPWRGVGPLPYSRGLSPADGVLRLGDAAAVGDP
                     FMGEGIGRALASGPMLVAALR"
BASE COUNT          142 a          494 c          463 g          213 t
ORIGIN      
        1 gcccgttccg gccatcgccg cccgcgcccg tcgggcctgc gccggttacc ccgaaatcga
       61 catcctcgaa aaagaccttc gatggatggt cgatcgcggc gcgcggttcg attacgtgac
      121 ggcgtccctc tttctccacc acgtatccct tcgggagcgg gccgacgtgc tggccgccct
      181 ggaccgcttg gcccgccggg ggttgatcct cagcgacctc cggcgaagcc ttccgggcta
      241 ctgggccgtg ggcgccctgg cggccctgcg gcgggaccgg gtggccttcc acgacggtcc
      301 tctgtcggtg cgtcgggcct tccgggtcgc cgacctcgaa aacttggccg ccacggcggg
      361 cctgccccac ctccgggccc ggtcggaacc gtggttccgg gtcagcctgc tcggtgagaa
      421 aaatggctga acgcgcggtg gtcgtcggcg gcggaccggc gggcgcgagc gcggccctgg
      481 cgcttttggc ggcgcgattc gaggtcacgg ttttcgagcg ccgcccccgt ttcgcccccc
      541 gggtttgcgg aacctttttg gacgccgagg ccctccggca cttaaccacc ctgggggttc
      601 gggacctcat cgaccgggaa tccgttcccg ttcgacgggt tcgcgtgacg gttccccccc
      661 ggcgcctccg cgtcgtcgat ctgcccgagg ccggggccgc cctgccccgt ccccgtctgg
      721 aagccttgct gttcgacgaa attcggcggc ggggcggggc cgtcgtcccg ggcgtccggg
      781 tttttcccgg cgaagattcc cgaaccgtcc ggctggacgg tcccggactc ggggagggcg
      841 gaaccccggt tcggcgcgcc gacctcgtgg tgtgggccac cggccggccc gcgcccgacc
      901 ccgccggcgg cggccccggc cactacggct ggaacgccga cttcgacggg gtggaccagc
      961 gtcccgggga catgtccctc cacttcacgc cccggggcta cgtgggggtc gtgacccacg
     1021 gcgacgggac gaccaacgtc tgcggcctct accggcggga gaacgtcccc cccgactggg
     1081 aaaacgtttt tcgggaatcg cggaacgact ccccggcctt cgcccgggag acggccgcgg
     1141 cccgccgccg gaccccctgg cggggcgtcg gtcccctgcc ctattcccgg ggactctctc
     1201 ccgccgacgg cgtcctccgt ttgggggacg ccgccgccgt gggggatcct ttcatgggcg
     1261 aaggcatcgg ccgggccctg gcctccggac cgatgctcgt ggcggccctg cg
//
LOCUS       DAOQLS010000686         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_87866_length_1312_cov_2.192522, whole genome shotgun sequence.
ACCESSION   DAOQLS010000686 DAOQLS010000000
VERSION     DAOQLS010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87866_length_1312_cov_2.192522"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK362_10650"
     CDS             complement(<1..679)
                     /locus_tag="PK362_10650"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNC75327.1"
                     /translation="MKTAFLFPGQGSQYVGMGRELAENFPAARAIIDRAAAKLGAEYI
                     DVFLNGPEEKLRQTRFTQVSLYIVSLAAHEVLKGAGFKADAVAGHSLGEYSALAAAGA
                     FDFETGLELVKARGEAIGAAAEKVPGTMAAIVGLERNVVEDLCQKASTHGVCQAVNYN
                     CPGQIVVAGEVAAVQEAVKAAQAAGSPKSIVLNVSGPFHSSLMKPAADVMAGVLGRAT
                     IVSPAVPV"
     gene            complement(676..>1312)
                     /locus_tag="PK362_10655"
     CDS             complement(676..>1312)
                     /locus_tag="PK362_10655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC75328.1"
                     /translation="VHRALAPEGSFFLNVGGTPQNPWIPADVANRLRSIFVLQNQIVW
                     VKSLATGDEDDPTGRRPGLSLGHYKPVNSRRYLHSGHEFVFHFTKTGRVPLDRLAIGV
                     PYRDKSNIARWGRSADRRCRGNVWFVPYRTIQSRKGDRPHPATFPVELPVLCLKLHGL
                     ERVRTVLDPFLGLGSTGRAARSLGLNFVGFDIDATYVAEARRALTKAGANA"
BASE COUNT          244 a          461 c          395 g          212 t
ORIGIN      
        1 agaccggcac ggccggcgaa acgatcgtcg cgcggcccaa caccccggcc atgacgtccg
       61 ccgcgggttt catgagagag gagtggaacg gccccgacac gttcaacacg atggacttgg
      121 gcgaaccggc cgcctgggcg gctttgacgg cttcttgaac ggccgcgacc tccccggcca
      181 cgacgatttg gcccgggcag ttgtaattga ccgcctggca aacgccgtgg gtcgaggcct
      241 tttggcaaag gtcttccacc acgttccgct ccagacccac gatggccgcc atggtgcccg
      301 gcactttttc ggccgcggcc ccgatggcct cgccccgggc cttcaccaac tccaacccgg
      361 tctcaaaatc gaaagccccc gccgcggcca gggccgaata ttcgcccagg ctgtggcccg
      421 ccaccgcgtc ggccttgaaa ccggcccctt tcaacacttc gtgggccgcc agggagacaa
      481 tatagagaga cacctgcgtg aagcgggttt gccgaagctt ttcctccggg ccgtttaaaa
      541 agacgtcgat gtactccgcc ccgagtttgg ccgccgcccg gtcgatgatc gcccgggccg
      601 cggggaagtt ctccgccaat tcccgcccca tgcccacgta ttgggacccc tgccccggaa
      661 acagaaaagc cgttttcatg cgttcgctcc cgctttcgtc agcgcccgtc gggcttccgc
      721 cacgtaggtc gcgtcgatgt caaaccccac aaaattcaac cccagggacc gggccgcgcg
      781 gcccgtggat cccaaaccga gaaaggggtc caacaccgtg cggacccgct ccaggccgtg
      841 cagcttgagg cacaaaaccg gaagctccac cggaaacgtc gccggatggg gccggtcccc
      901 cttccgggac tggatggttc gatacggcac gaaccacacg ttgccccggc accgccgatc
      961 cgccgaccgg ccccaccggg cgatgttcga tttgtcccgg tacgggaccc cgatggccaa
     1021 ccggtccagg ggaacccgac cggtcttggt gaaatggaac acgaattcgt gcccgctgtg
     1081 caaatagcgc cgactgttga ccggcttgta atgccccagg gaaagcccgg ggcgccggcc
     1141 cgtggggtcg tcctcgtccc cggtggccag ggatttgacc cacacgattt ggttctgcaa
     1201 aacaaaaatg gatcggagcc ggttcgccac gtccgcggga atccagggat tttggggcgt
     1261 cccccccacg ttcaaaaaga aagacccctc gggggcgagc gcccggtgaa ct
//
LOCUS       DAOQLS010000687         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_88234_length_1309_cov_3.155502, whole genome shotgun sequence.
ACCESSION   DAOQLS010000687 DAOQLS010000000
VERSION     DAOQLS010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88234_length_1309_cov_3.155502"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK362_10660"
     CDS             complement(<1..414)
                     /locus_tag="PK362_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNC75329.1"
                     /translation="MNAPGDLAKDLARFWADVEGDTLLVHTDVARLNTPLPPAPRDVQ
                     LERLFDILRSAAGGRTLLLPTFNYDYPRTRVYDVRGDAGQVGVLNDHIRRRPGVVRTR
                     TPIFNFAVVGGGWSPAANENPFSAESVFGECVRRRR"
     gene            complement(422..>1309)
                     /locus_tag="PK362_10665"
     CDS             complement(422..>1309)
                     /locus_tag="PK362_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75330.1"
                     /translation="DEAVVRDAFDRHPDMVLRWTDFAAARINWDDKAKNIADLAAELR
                     LGLNAAVFLDDHPAERDRVRNALPEVLVPDWPADPALAPQALRALAAFDAVAVSEEDR
                     RRTALYAEEKARAAAAPGGSRDAWLKSLGATVWVNRLNDADLTRAAQLLNKTNQMNLA
                     TRRLTEGELRAWAAPAHRRFFTFRVADKFGDAGLVGLGSVEVEQGKARVTDFVLSCRV
                     LGRGVERTVLSVLAAEAKALGAATVTATLVPTERNRPCAEFWERESGFEKSPDGAYVL
                     DLARAYPAPDHLTVARASA"
BASE COUNT          196 a          466 c          447 g          200 t
ORIGIN      
        1 ccttctccgc cggacgcact cgccgaaaac ggattcggcg gagaaggggt tctcgttcgc
       61 cgccggggac cacccgccgc ccaccacggc gaaattgaaa atcggcgtcc gggtccggac
      121 gacgcccggc cgccggcgga tgtggtcgtt caacaccccc acctgaccgg cgtccccgcg
      181 cacgtcgtac acccgggtgc gggggtagtc gtagttgaac gtgggcaaca gcagggtccg
      241 tccccccgcc gcggaccgta gaatgtcaaa caaccgctcc aattgaacgt cccggggcgc
      301 cgggggcagc ggggtgttga gccgggccac gtccgtatgg accagcaacg tgtccccctc
      361 cacgtcggcc cagaaccggg ccaggtcctt ggccaaatcg cccggggcgt tcatcggcgt
      421 ttcacgcgga ggctcgggcc accgtcaggt ggtccggcgc gggataggcc cgggccagat
      481 cgaggacgta ggcgccgtcc gggctttttt caaaaccgga ttcccgctcc caaaattccg
      541 cgcaggggcg gttgcgttcg gtcgggacca gggtcgccgt cacggtcgcg gcgcccaggg
      601 ccttggcctc ggccgccagg acgctcagga cggtccgctc gacgccgcgg cccagaacgc
      661 ggcagcttaa aacgaagtcc gtgacgcgcg cttttccttg ctcgacttcg acgcttccca
      721 accccacgag ccccgcgtcg ccgaacttgt cggcgacccg aaaggtgaag aaacgccggt
      781 gggcgggggc ggcccaggcg cgcagttcgc cttccgtcaa gcgccgggtc gccaaattca
      841 tttgattggt cttgttcagc aactgggcgg cccgggtcaa atcggcgtcg ttcaagcgat
      901 tcacccaaac ggtcgcgccg agggatttca gccaggcgtc gcggcttccg cccggggccg
      961 ccgccgcccg ggctttctct tccgcgtaca aggccgttcg ccgccggtcc tcctccgaca
     1021 ccgccaccgc gtcgaaggcg gccagggcgc ggagggcctg gggggccagg gccgggtccg
     1081 ccggccagtc cgggacgagc acctcgggca gggcgttgcg cacccggtcc cgctccgcgg
     1141 gatgatcgtc gaggaagacg gccgcgttta ggcccagccg cagttccgcc gccaggtccg
     1201 cgatgttctt ggctttgtcg tcccaattga tccgcgcggc ggcgaaatcg gtccagcgca
     1261 gcaccatgtc cgggtggcgg tcgaaggcgt cccggaccac ggcctcgtc
//
LOCUS       DAOQLS010000688         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_88244_length_1309_cov_2.830144, whole genome shotgun sequence.
ACCESSION   DAOQLS010000688 DAOQLS010000000
VERSION     DAOQLS010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88244_length_1309_cov_2.830144"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PK362_10670"
     CDS             complement(<1..337)
                     /locus_tag="PK362_10670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNC75331.1"
                     /translation="MSRAPRVSVVMAAYNAEAFIEAAIESALAQTLPADVWELLVVDD
                     GSTDGTRARAARFGEPVRWIEQAHAGLVPACNRGLREARGEFFARLDADDVVDPRWLE
                     RCLAALEKHP"
     gene            complement(369..1304)
                     /locus_tag="PK362_10675"
     CDS             complement(369..1304)
                     /locus_tag="PK362_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC75332.1"
                     /translation="MVTGGSGFIGSHVVDRLIAKGIGVRVFDMVLPRYGQNIEFYKGS
                     LLDLESVRLALNGVDVVFHLAAIADVNDVVKDPFYAEAINVRGTANLLEAIRRSSGIR
                     RLVYGSTTWVYSAAKETNVDEDTALVAPDHFYTATKITSEYYCHSYGKLFNIPTTILR
                     YGIPYGPRAREAAVIPVFVKKAFNKEPLTIAGDGMQFRKFVYVEDLAEGNVLALQAAG
                     KNRVYNLDGQEKVTIKQIAETIQKVVGDVKIEYTPARAGDFGGKEVNSDRAAAELGWK
                     PKVAFEEGVRRYVAWLKEDMDRRKKEWDRVDPALR"
BASE COUNT          207 a          459 c          403 g          240 t
ORIGIN      
        1 cgggatgctt ctccagggcc gccaggcagc gctccagcca ccgggggtcg accacgtcgt
       61 cggcgtccaa gcgcgcgaaa aattcgcccc gggcctcccg caggccccgg ttgcaggccg
      121 ggaccagccc cgcgtgggcc tgctcgatcc accgcaccgg ttcgccgaac cgggcggcgc
      181 gcgcccgggt gccgtcggtg gacccgtcgt ccacgaccaa cagctcccag acgtcggccg
      241 ggagcgtctg agccagggcg ctttcgatgg ccgcctcgat gaacgcctcg gcgttgtacg
      301 cggccatcac gacgctgacc cgcggcgcgc ggctcatcgg gttcccccgc cgggggcggt
      361 tcgccccgtc agcgcaaggc ggggtcgacc cggtcccact cttttttccg gcggtccatg
      421 tcctctttca accacgccac gtaacggcgc acgccttcct cgaaagccac cttgggcttc
      481 cagcccaatt cggcggcggc ccggtcgctg ttgacttcct tgccgccgaa gtcgccggcg
      541 cgggccgggg tgtattcgat cttcacgtcg ccgacaacct tttggatggt ctcggcgatt
      601 tgcttgatcg tgaccttttc ctggccgtcg aggttgtaga cccggttttt gcccgccgcc
      661 tgcagggcca gcacgttccc ttcggccaaa tcctccacgt agacgaactt gcggaactgc
      721 atcccgtccc cggcgatggt caggggctcc ttgttgaagg ccttcttaac aaacaccggg
      781 atcaccgccg cttcgcgggc ccgcgggccg tagggaatgc cgtagcggag gatggtggtc
      841 gggatgttga acagcttccc gtaggaatgg cagtagtact cggaggtgat tttggtggcc
      901 gtgtaaaaat ggtccggggc caccagcgcc gtgtcctcgt ccacgtttgt ttccttggcc
      961 gcgctgtaga cccaggtggt gctgccgtag accaaccgtc gaatgcccga ggaacggcgg
     1021 atggcctcca acaagttggc cgtgccccgc acgttgatgg cctcggcgta aaacgggtcc
     1081 ttgacgacgt cgttgacgtc ggcgatcgcg gccaggtgaa agaccacgtc gaccccgttc
     1141 aacgccagcc ggaccgattc caaatccagc aagctcccct tgtaaaactc gatgttctgc
     1201 ccgtaccgcg gcaacaccat gtcgaacacc cgcacgccga tgcctttggc gatcagccga
     1261 tccaccacgt gcgacccgat aaaccccgac ccgccggtga ccaacgctt
//
LOCUS       DAOQLS010000689         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_89437_length_1300_cov_2.302008, whole genome shotgun sequence.
ACCESSION   DAOQLS010000689 DAOQLS010000000
VERSION     DAOQLS010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89437_length_1300_cov_2.302008"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..623
                     /locus_tag="PK362_10680"
     CDS             129..623
                     /locus_tag="PK362_10680"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC75333.1"
                     /translation="MNARALEAVALLARDRPDWQYIHLTGASDEAKVRAAYGDLSRRA
                     FVRGFHSDMATVYSAADFVVARAGANTVMEIRRMGRPALLVPFPYATDDHQTANAKFL
                     EQTGSVRVVQERDLSVEAFLSILKTLPDVRALRSQAAERLAHAGSEMLTAAKRAADLI
                     MEAK"
     gene            620..>1300
                     /gene="surE"
                     /locus_tag="PK362_10685"
     CDS             620..>1300
                     /gene="surE"
                     /locus_tag="PK362_10685"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC75334.1"
                     /translation="MKRAQKNILVVNDDGVYGEGLKPLARALGRLGRVTIVVPERERS
                     AASHAITLHKPIRVRKLEPNLYIMNGTPADCTRFGVIALLKEKCDLVVSGINHGANLG
                     ADTMYSGTVGAAKEACMLGIPALAVSVTTKEEKPHFDTAARFAVDAARQILARGLPKH
                     TCLNVNVPNHPWRLLKGVAVTTLGQRIYGRALSPRVDPRGLTYYWMAGAVPRGVAEPG
                     SDIEAIEAG"
BASE COUNT          235 a          407 c          421 g          237 t
ORIGIN      
        1 gtattcctga gcgccgatcc cgaggtcgcg ggctactttg aggcgctgac cggcgagggc
       61 gttgcgccgc gcaccgccat gcactggctg acgacccagc tactcccggc agtcaaggcg
      121 cgcgggcttt gaacgcccgg gcgttggagg ccgtggccct tctggcccgt gaccgacccg
      181 attggcagta catccatctc accggcgcta gcgatgaggc gaaggttcgg gccgcctacg
      241 gggacctttc ccggcgggcg ttcgttcgcg ggttccattc ggacatggcc acggtttatt
      301 ccgcggcgga tttcgtggtg gctcgggcgg gcgccaatac ggtgatggaa atccgccgca
      361 tgggccgacc ggcgttgttg gtcccgttcc cctatgccac cgacgaccat cagaccgcga
      421 acgctaaatt tttggaacaa acgggttcgg tccgcgtggt tcaagaacgg gatctcagcg
      481 tcgaagcttt tctgtccatc ttgaaaacgt tgcccgacgt ccgggctttg cgatcccagg
      541 cggcggagcg gctggctcac gcgggttcgg aaatgttgac cgccgccaaa cgggcggcgg
      601 atttaataat ggaggccaag tgaaacgagc acaaaagaat attttggtgg tgaacgacga
      661 cggcgtttac ggggaagggt tgaaacccct ggcccgggcg ttggggcgat tggggcgggt
      721 gaccatcgtc gtgccggaac gggaacggtc ggcggccagc cacgccatca cgcttcacaa
      781 acccatccgc gtgcgaaaac tggaaccgaa cctctatatc atgaacggca ctccggcgga
      841 ctgcacccgg ttcggcgtca tcgccctgct gaaagaaaag tgcgacctgg tggtctccgg
      901 cattaaccac ggagcgaact tgggcgccga caccatgtat tccggtaccg tgggcgccgc
      961 caaggaagcg tgcatgttgg gcatccccgc cctggcggtt tcggtgacaa cgaaagagga
     1021 aaagccccac ttcgacaccg ccgcccgctt cgccgtagat gcggcccgcc aaattttggc
     1081 ccggggtttg ccgaaacaca cctgcttgaa tgtgaatgtg cccaaccatc cgtggcggtt
     1141 attgaaaggc gtggccgtca ccaccttggg ccagcgcatc tacggccgcg ccctgtcccc
     1201 ccgcgtggat ccccggggcc tcacctacta ttggatggcc ggcgccgtcc cccgcggcgt
     1261 cgccgaaccc ggttccgaca tcgaggcgat tgaggcggga
//
LOCUS       DAOQLS010000690         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_89822_length_1297_cov_3.559581, whole genome shotgun sequence.
ACCESSION   DAOQLS010000690 DAOQLS010000000
VERSION     DAOQLS010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89822_length_1297_cov_3.559581"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK362_10690"
     CDS             <1..263
                     /locus_tag="PK362_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75335.1"
                     /translation="SIQQYGCVYVWAHSQGTAIAKAALSMLTLAERSRVIYEGAGPQT
                     YVDGKKLGLKEAANIRYPKDPIPITNDVLKWIGMRPKNQDWI"
     gene            254..577
                     /locus_tag="PK362_10695"
     CDS             254..577
                     /locus_tag="PK362_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75336.1"
                     /translation="MDLNKEALILAKPGSSGVPALKDIPAQLPAKYDGGVIHGVFSAG
                     GVFQITHVERVKSFEFTFVDFYATVMSEGNFKGQKVDVGGLTIQNPSAPIFNQKYVEE
                     INPAK"
     gene            940..>1297
                     /locus_tag="PK362_10700"
     CDS             940..>1297
                     /locus_tag="PK362_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75337.1"
                     /translation="MPFVVRSEPQSRREDAILVVTNHTTQDLYIRVSLFDMDGNEMRR
                     GPLAGVGAGNLFPDFGFPLSATVRHHVPHIGAERHGERSVNAAPAKLKTLVSAADGVH
                     DQADLVQRPHGGRQGKA"
BASE COUNT          308 a          322 c          348 g          319 t
ORIGIN      
        1 attcgatcca gcaatatgga tgtgtttatg tttgggccca ctcccaagga acagctatcg
       61 cgaaggctgc cctgtcgatg ctgactttgg cggaaaggtc tcgagtgata tatgaaggcg
      121 ctggccctca gacttatgtg gacggtaaaa aacttggatt aaaagaagct gcgaatattc
      181 gatatccaaa agatccgatc ccaatcacta atgatgtttt aaaatggatt gggatgcgtc
      241 ctaaaaacca ggattggatt taaataagga agctttgata ttagctaagc ctgggtcatc
      301 cggggtgcct gccctaaagg atattccggc acagcttccc gcaaaatacg acggtggggt
      361 tattcatgga gtgttttcgg ccggtggagt ttttcaaatt actcatgttg agcgtgtgaa
      421 gtcatttgaa tttactttcg tggactttta cgcgacagta atgagcgagg ggaattttaa
      481 gggccaaaag gtcgatgttg gagggcttac aattcagaat ccttctgctc cgattttcaa
      541 ccagaaatac gtcgaagaaa ttaatcctgc caaatagaaa ttcaattcaa tcgatcccat
      601 atagcgcggt aattttcgtc gtgtaagtgt aatgctcccc attgaccaag ccgggtttaa
      661 gggggtaccg gggttgggtg ttgggagagg aggctccatc cccggggtgg gaatggatat
      721 gaagtaaatt agtaggcaac gtgccctagt tcctcctata agtttggtct cctccccggc
      781 catttccggc ggcctcccca tttcataagc tttcgtaact ttattcctcc ggaaaggtac
      841 tccatgcccc ccgtgatatt gccttacacg tacattcttc ccgactggcg ggagatcaca
      901 aatattcagc cccggccgga ggacccgcca attttcctct tgccgtttgt cgtgcgcagc
      961 gaaccccagt cgcgaaggga agacgccatc ctggtggtga ccaaccacac gactcaagac
     1021 ctctacattc gcgtctcgct ttttgacatg gacggcaacg aaatgcgccg gggccccttg
     1081 gcgggggttg gcgcggggaa tctgttcccg gacttcggct ttcccttgtc ggccaccgtg
     1141 cggcatcacg ttccacacat aggcgctgag cgccacgggg aacgaagtgt gaacgcggcc
     1201 ccagccaaac ttaaaacgct ggtttccgct gccgatgggg tccatgacca ggcggacctg
     1261 gttcaaaggc cgcacggtgg ccgacaaggg aaagccg
//
LOCUS       DAOQLS010000691         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_89950_length_1296_cov_4.126511, whole genome shotgun sequence.
ACCESSION   DAOQLS010000691 DAOQLS010000000
VERSION     DAOQLS010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89950_length_1296_cov_4.126511"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PK362_10705"
     CDS             <1..687
                     /locus_tag="PK362_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC75338.1"
                     /translation="MDAINAWAQSHRLPVVEDACQAHGARWRGRPVGSLGAVGCFSFY
                     PTKNLGGFGDGGMAVTNDPALADRLRSLRNCGRRAQYEHLELGYNERLDNLQAALLRL
                     KLPRLVGWTEERRRLAEEYRRGLEGAPARPLAVAPGAEPVYHLFTVRAPRRDALKAFL
                     AERGVQSGVFYPTPLHLQPAFKSLGGKVGDCPEAERAAQDVLSLPLYPGLPAASVQRV
                     AALVREFYRV"
     gene            complement(684..>1296)
                     /locus_tag="PK362_10710"
     CDS             complement(684..>1296)
                     /locus_tag="PK362_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75339.1"
                     /translation="KTRRWRPILAGLPAALLLCFHLANNQRRNGDALYSINLHSYWYA
                     NLEFVGRPGFPATEAERQRDVYRPSLNFRRWAFEAHTPAQYLAGTIQGYGRIAWTFFA
                     QVYYRVGLPALLTGALLALHLWGLLGALGGTPGRVALAAWVLLNFPYAFVGHVFWAGR
                     FFVPFAPLALGLLVAGARDLTRRARAGWASFLLEERGAGRKIR"
BASE COUNT          229 a          420 c          442 g          205 t
ORIGIN      
        1 atggacgcca tcaacgcctg ggcccaaagc catcggcttc ccgtggtgga ggacgcctgc
       61 caggcccacg gcgcccgctg gcggggccgt cccgtggggt cgctcggggc cgtcggctgt
      121 ttcagttttt atccgaccaa aaacctgggg gggttcggcg acggcggcat ggccgttacc
      181 aacgacccgg ccctggccga ccgactgcga agtttaagga actgcggacg gcgggcccag
      241 tacgaacatt tggagctggg gtacaacgag cggttggaca atttgcaggc ggccctgttg
      301 cgactcaaac tgccgcgtct ggtgggatgg acggaggagc ggcgtcgatt ggccgaggaa
      361 taccggcggg gtctcgaggg cgcccccgcc cgccccttgg ccgtcgcccc cggggcggaa
      421 ccggtttatc atctgttcac cgttcgggcc ccccggcggg acgcgctcaa ggccttcctg
      481 gccgaacggg gcgttcagag cggcgttttc tacccgaccc cgctccatct tcagccggcg
      541 ttcaaatccc tcgggggaaa agtcggggat tgtcccgaag ccgaacgggc cgcccaggac
      601 gtgttgtcgt tgccccttta ccccggtttg cccgcggcgt cggtccaacg tgtggcggcc
      661 ttggtgcggg aattctaccg cgtctagcga atttttcgcc cggcgccccg ttcttctaag
      721 agaaaggacg cccaccccgc ccgggcccgt cgcgtcaaat cccgcgcccc ggcgacgaga
      781 agccccagag ccaagggggc gaaggggacg aagaaacgcc ccgcccaaaa aacatggccc
      841 acaaaggcgt acggaaaatt taacaggacc cacgccgcca gggcgacccg gcccggcgtc
      901 ccgccgagcg ccccgagcaa tccccacaga tgaagggcca ggagcgctcc ggtgagcagg
      961 gcggggaggc cgacccggta ataaacctgg gcgaaaaaag tccaggcgat ccgcccgtac
     1021 ccctggatcg tcccggccag gtattgcgcc ggggtgtggg cttcgaaggc ccatcgtcgg
     1081 aagttgaggc tggggcggta aacgtcccgt tgacgttcgg cctcggtggc cggaaaaccc
     1141 ggccggccca cgaattccag gttggcgtac caataactgt gcagattgat cgaatagagc
     1201 gcgtcgccgt ttcgtcgttg gttgttggcc agatggaaac acaggagaag cgcggcgggg
     1261 aggcccgcca ggatcggccg ccaccggcgg gttttt
//
LOCUS       DAOQLS010000692         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_89998_length_1296_cov_2.767929, whole genome shotgun sequence.
ACCESSION   DAOQLS010000692 DAOQLS010000000
VERSION     DAOQLS010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89998_length_1296_cov_2.767929"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PK362_10715"
     CDS             complement(<1..559)
                     /locus_tag="PK362_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75340.1"
                     /translation="MIGRIFSFQRSAVPWAFFTGGIAVALFTGLAGHTDPAGLRVQTQ
                     QFLIPTVIVMIALGAFVTIFRGEAQGEASLGTTLSDLVVTAVLWGGPLVAGLVTLAQW
                     WQGRPCFPPPEITNFAIVFALAGAGFAVCFHGVRVPLLLWHKWPPLRYGTILVLAFWV
                     VYTGPAFVARVSADLTVREDRRRRLE"
     gene            838..975
                     /locus_tag="PK362_10720"
                     /pseudo
     CDS             838..975
                     /locus_tag="PK362_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            993..>1296
                     /locus_tag="PK362_10725"
     CDS             993..>1296
                     /locus_tag="PK362_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75341.1"
                     /translation="MLPPVVFLSLRQWEETLKLFPQRRGRRLAGWLTVLALAGGSLGL
                     ADYLQAETGRWIIRDVTAAGWLGRGRTGYFLGDSFTGSDLKDAGWKPAFESTVFRPG"
BASE COUNT          260 a          398 c          398 g          240 t
ORIGIN      
        1 attccaatcg tcgtcgacgg tcttcccgca cggtcaggtc cgccgacacc cgcgccacga
       61 aggccggccc ggtgtaaacc acccaaaagg ccaacaccag gatcgtcccg tatcgaaggg
      121 gcggccattt gtgccacagc aggagcggca cccgcacccc gtggaagcac acggcgaacc
      181 cggcccccgc caaggcgaaa acgatggcga agttggtgat ttccggcggc ggaaaacagg
      241 gccgcccctg ccaccactgg gccagggtca cgagccccgc caccaagggc ccgccccaga
      301 ggaccgccgt caccaccaag tcggaaaggg tcgtccccag ggaggcttcc ccctgggctt
      361 cgccgcgaaa aatcgtcaca aaggcgccca gggcgatcat cacgatcacc gtcggaatca
      421 ggaattgttg ggtttgaacg cgaaggccgg cggggtcggt gtggccggcc aggccggtga
      481 agagggcgac ggcgatcccc ccggtgaaaa aagcccaggg caccgcgctg cgttgaaagg
      541 aaaagatgcg accgatcatg ggtgctcccc gaggtgcccc gtcgaaatgc gtccggcttc
      601 gattgaatta tccaattaaa aaagtggatt gcaaacgttt taggtgggcg aaatcggctt
      661 tccgtcgtcc cttataaaaa atgacaattc cgatgaaaaa gcctctttcc gcacccggaa
      721 aatagttgac tgacatcggt ttataaggca acccgttgat atttcgccct cgtcggatta
      781 ggcttaatta tgccaaaaca ggcgcttagg aacaggccct ttcacggcgt gctatatatt
      841 ccgacgtgcc ctcggcctac ccaccgccca gatggggggg ccggatttac tctcatcgag
      901 ttgatgctcg tggtggcgat catcggtctt ttggcggcca tcgccatccc gaaattcgga
      961 aaccttgggt cgcggcccgc tattacctgg ccttgctccc gcccgtcgtt ttcctttcgc
     1021 ttcgccaatg ggaggagacg ttgaaacttt tcccccagcg gcggggccgg cgtctggccg
     1081 gttggctgac ggtcctggcc ctggccggag ggagtctggg cctcgccgat tatttgcagg
     1141 cggaaaccgg acggtggatc atccgcgacg tgaccgccgc gggatggttg ggccgcggcc
     1201 gaacgggtta tttcctgggg gattccttta ccggcagcga tctgaaggac gccgggtgga
     1261 aaccggcctt tgaatccacg gttttccgcc cggggg
//
LOCUS       DAOQLS010000693         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_90515_length_1292_cov_3.240905, whole genome shotgun sequence.
ACCESSION   DAOQLS010000693 DAOQLS010000000
VERSION     DAOQLS010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90515_length_1292_cov_3.240905"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK362_10730"
     CDS             <1..230
                     /locus_tag="PK362_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75342.1"
                     /translation="GTYSFTQPAISQSVGARKIDRILSTGAAVVLTSSVSCLLQLRAG
                     LRRAGSRVRALHIASFFSPDRPVPPGSAGDL"
     gene            348..668
                     /gene="trxA"
                     /locus_tag="PK362_10735"
     CDS             348..668
                     /gene="trxA"
                     /locus_tag="PK362_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNC75343.1"
                     /translation="MEVQLSDSAFDKDVLKSPKPVLVDFWAPWCGPCRVLGPIVEELA
                     KEYDGKIVVGKLNTDDNPNTASHYNISAIPTMLFFKGGQVVDQMVGVYSKKDIKAKLD
                     GLLK"
     gene            675..1241
                     /locus_tag="PK362_10740"
     CDS             675..1241
                     /locus_tag="PK362_10740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC75344.1"
                     /translation="MNRRFLPLAALPLLWPALGGCGRPEHGGGTAAVDASATGAPVFS
                     LPNLEGKTVSLAEYKGRVVFLDFWATWCPPCRASVPEVEKLYGDYRGQNLQILGMNLD
                     EDASDVVRFVKKKGVSYPVILAGESGVSDLYGVRGIPHFVLIDKNGKVAEWWAGYSLD
                     MPGAWRTAIDRLLVAPPAAPAQSGPRTY"
BASE COUNT          206 a          452 c          394 g          240 t
ORIGIN      
        1 gcggcaccta cagtttcacc caacccgcga tttcccagtc cgtgggcgcc cggaagatcg
       61 accggatcct ctccacgggc gcggcggtcg tcctgacgtc gagcgtgtcc tgcctgcttc
      121 aattgcgggc gggccttcgc cgcgccgggt cccgggtccg ggccctccac atcgcctcgt
      181 ttttttcccc ggaccggccg gtccccccgg gttccgcggg cgatctttga cagccccccg
      241 ccggtgtggt aaactgcccg cgcaggcgcc gcctcgaggg aactttcccc cgggcggcgc
      301 gttcaagtaa atgatccccc tttgccccaa tccgaaagga gcctttcatg gaagtccaac
      361 tcagcgacag cgcttttgac aaagacgttt tgaaatcccc caaacccgtg ctggtggact
      421 tttgggcgcc ctggtgcggc ccctgccgcg tcctggggcc gatcgtggag gaactcgcca
      481 aggaatacga cgggaagatc gtcgtgggca agctcaacac cgacgacaac cccaacaccg
      541 cctcccatta caacatctcc gccattccga ccatgctctt cttcaaaggc ggccaggtgg
      601 tggatcagat ggtcggcgtt tactccaaaa aagacatcaa agccaagctc gacggcctgc
      661 tgaagtaggt ccgtttgaac cgccgcttcc tcccgctggc cgccctgccg ttgttgtggc
      721 ccgccttggg ggggtgcggc cgtccggagc acgggggcgg gacggccgcc gtcgacgcct
      781 ccgccacggg cgcgccggtc ttttccctgc cgaatttgga ggggaaaacc gtgtccctgg
      841 cggaatacaa ggggcgcgtg gtgttcctgg atttttgggc cacctggtgt cccccgtgcc
      901 gggcctcggt gcccgaagtg gaaaagcttt acggcgatta ccggggccag aacctccaaa
      961 tcctcggaat gaatttggac gaggacgcct ccgacgtcgt ccggtttgtt aagaaaaaag
     1021 gcgtctccta ccccgtgatc ctcgccggtg agtccggggt ctccgacctc tacggcgtgc
     1081 gcggcatccc gcacttcgtg ctcatcgaca aaaacggcaa ggtggcggag tggtgggccg
     1141 gctattccct cgacatgccg ggggcctggc ggacggccat cgaccggctt ttggtggcgc
     1201 cccccgccgc gccggcccag agcggccccc gcacctactg atcttcccgc cgggacaacc
     1261 gtgacgagtc ccgcccgccc ccgcttctgc ct
//
LOCUS       DAOQLS010000694         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_90654_length_1291_cov_3.415049, whole genome shotgun sequence.
ACCESSION   DAOQLS010000694 DAOQLS010000000
VERSION     DAOQLS010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90654_length_1291_cov_3.415049"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1291
                     /gene="murJ"
                     /locus_tag="PK362_10745"
     CDS             <1..>1291
                     /gene="murJ"
                     /locus_tag="PK362_10745"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNC75345.1"
                     /translation="DALVANAFGGGHLTDAFYAAFRLSNLFRRILGEGPLATAFVPVF
                     SDHLARKERAEANNFFYTLFTFLTVLLLGIVALGILGSPWIVSLAAGGFKTADLSKYE
                     LTVRLTRQLFPFLLFVCLAALSSAALNALGYFFIPSLAPAMLSVATIVYILFIHRWTV
                     DPIQGLAISTTVGGILHFLMLWPQLKKEGLLFRWKWNPRDPDVRRVGWLVLPSIWGLS
                     IDQVNAYVDTICASFLAEGSVTALYNSNRLMQFSLALFGVSLSTATLPHLALSAAKGD
                     WEGFKENLNFSIRLTLYMVVPATVGMVLLARPLVQLLFEHGRFTAVQTGYTAAALAAY
                     TLGLPAYSLVKVVVTAFYAQKDTRTPVRVATLCLFINMVGNVVFMHYWGVGGLAFATT
                     LASFVNVGVLVHFLRKKIGLMGGRRIGASVLQMAGASL"
BASE COUNT          183 a          428 c          375 g          305 t
ORIGIN      
        1 gggacgccct ggtggccaac gccttcggcg gcggccacct gacggacgct ttttacgccg
       61 ccttccgcct ctcgaacctg tttcggcgca ttctggggga gggccccctc gccacggcct
      121 tcgtcccggt gttttcggac cacctcgccc gaaaggaacg ggcggaagcc aataatttct
      181 tttacaccct tttcaccttc ctgacggtcc tgcttctggg catcgtggcc ctggggatat
      241 tgggttcgcc ctggatcgtt tccctggcgg ccggggggtt taaaaccgcg gacctctcca
      301 aatacgagtt gaccgtccgg ctgacccgcc agctgtttcc ctttttgttg ttcgtctgtt
      361 tggcggccct gtcctcggcc gcgttgaacg ccctgggata ttttttcatc ccgtccctgg
      421 cgcccgccat gctgtcggtg gccaccatcg tctacatcct tttcatccac cgctggaccg
      481 tcgatcccat tcagggcttg gcgatcagca ccaccgtggg gggaatcctc catttcctga
      541 tgttgtggcc ccaattgaaa aaggagggtc tgctgttccg ttggaaatgg aacccccggg
      601 accccgacgt ccgccgcgtg ggctggctcg tgctcccctc catctggggc ctgtccatcg
      661 accaggtgaa cgcctacgtg gacaccatct gcgcctcgtt cctggccgaa ggctcggtga
      721 ccgccctcta caattcgaac cggcttatgc aattttccct ggcccttttc ggcgtgtcgc
      781 tttcgacggc gacgttgccc cacctggccc tgagcgccgc caagggggat tgggaggggt
      841 tcaaagagaa cctcaatttt tccatccgcc tgactcttta catggtggtc cccgcgacgg
      901 tgggaatggt cctcctggcc cggcccctgg tgcagttgct ttttgaacac gggcggttca
      961 cggccgtgca aaccggttac accgccgcgg ccctggcggc ctacaccctg ggccttccgg
     1021 cctattccct ggtcaaggtg gtcgtcaccg ccttttacgc ccagaaggac acgcgcaccc
     1081 ccgtccgggt ggcgacgctg tgcctcttca tcaacatggt cgggaacgtg gtgttcatgc
     1141 attattgggg cgtgggcgga ttggccttcg cgacgacgct ggcgtctttc gtgaacgtcg
     1201 gggtcctggt tcactttctc cgaaaaaaaa tcggcctcat gggcgggcgt cgaatcggcg
     1261 cctcggtcct tcaaatggcg ggagcctccc t
//
LOCUS       DAOQLS010000695         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_91169_length_1287_cov_5.273539, whole genome shotgun sequence.
ACCESSION   DAOQLS010000695 DAOQLS010000000
VERSION     DAOQLS010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91169_length_1287_cov_5.273539"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK362_10750"
     CDS             complement(<1..226)
                     /locus_tag="PK362_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75346.1"
                     /translation="MGDLVDGLAPCVVRHGWWEAEPGIGRVTKKEESRVAKLKALGNG
                     QVPIQAATAFKILDEWFDPEADLKDFIGDRA"
BASE COUNT          219 a          441 c          332 g          295 t
ORIGIN      
        1 atgcccgatc tccaataaaa tcttttaggt cagcttctgg gtcgaaccat tcgtccaaga
       61 ttttgaacgc tgtcgccgct tggatcggga cttggccgtt gccaagagcc ttcaacttgg
      121 cgactctgct ctcttctttt ttcgtgacgc ggccgatacc cggctctgct tcccaccaac
      181 cgtgtcgcac cacgcaaggg gccagcccat caaccaggtc acccaccggg agtgaagagt
      241 cccgcccact tccgtttgta aatccctccc gccttgccca tgctcccccg cccagtgact
      301 catggacgcc cggggggtcg gccatgtttt taccgccgag tgaagtgtgt ccggcttccc
      361 gcggtgtttc ccgccgccgc tcgcccgcgg cgttggccac atccttaccg ccagggcgag
      421 cggcggcgtt gagaccttcc catccgcctg tctcttccgc caagtctcct cgttttcgtt
      481 tgtcaatttc ccggcccttg gcgttggcca catcttcaca aaattcgcca gctggtccat
      541 gtgagctcgg tcgccgcgcg tcagatgctt catgctgttt gcgcccttcc aatctctggc
      601 cgccggggta ggcaaggagc caccatctgt cccgtctgtg attggctccc acgtcggaag
      661 cccgtaagca acaccatcgc gcatcatacc cacgcgcggc caagtctccg agaacggttc
      721 ccaatccgtt gtgcaggatc gctgatacgt tttccaagaa caggaacctc ggtcgaacca
      781 catccgcgac acggaggact tctcggtaaa gacctgaccg cgtttcatca ccgaccccgg
      841 cacggtttcc ggcgacgcta atgtcctggc aagggaaccc cgcatggatg caatccactc
      901 gtcctttcca gtcggatgcg tcgaacatcc gaacgtctcc ctcgaacact tgcaggttcg
      961 ggaaccatcc ctcggcggct cgctctcgga ggacttggca acaataggcg tcccattcga
     1021 cggcacagat tgggtcgtgc ccgagaataa ggtcggcgag gagtcctccg ccagcccccg
     1081 cgaacaagtg caaggtttta agtggtcgca tttcattttc cacgcacgct gtctttcacg
     1141 gcttcaccgc cagcgtccca ttttcctttt ttcctttttt cctttttgtg aagctccaac
     1201 ccgtaacgga cgatcttttc tttcatttcg tccccggcgg cggccccggc ggcccaggcg
     1261 gcggcatagg cggcggcggc ccaggag
//
LOCUS       DAOQLS010000696         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_91175_length_1287_cov_4.354708, whole genome shotgun sequence.
ACCESSION   DAOQLS010000696 DAOQLS010000000
VERSION     DAOQLS010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91175_length_1287_cov_4.354708"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PK362_10755"
     CDS             <1..1099
                     /locus_tag="PK362_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75347.1"
                     /translation="ALETRPGEQKLARFYAAVTLPAPDGSVEVLLSEDLVRPAPRLLA
                     PLFAAARRLVLFHEAQRVRSLSRVLGPRGPWALTEPSRFLGQSLRDRWVARAPAPAPS
                     PAVPGPAPLGEALGRAAARAEAADFDGREILDALDRLVPVLSQNVETNGESAPPADLA
                     AQRDEFVEAFRRQWAAETDRPLSLAEARSALTSTVVQSRFDLPAEIPPAGPEVARDHL
                     VIVPLTFTARELASFVDAAVRVSRGRRIVFALPAGPSFQKTKAELFRLAGRAQIAPHV
                     AVFALPEGAVTTEGGPVLRLAVVARGLLGVQPSLITVPRSLAIDGSNLPEGPYRTALF
                     LLLDALLQAVPLRLGDLDTIERTARAVATSA"
BASE COUNT          161 a          474 c          429 g          223 t
ORIGIN      
        1 ggccctggaa acccggcccg gggaacaaaa gttggcccgg ttctacgccg cggtcaccct
       61 gccggctccc gacggaagcg tggaggtcct gttgagcgag gacctcgtcc ggcccgcgcc
      121 gcgccttctg gcccccctct tcgcggctgc ccggcgattg gtactcttcc acgaagccca
      181 acgggtccgt tccctgtccc gcgttctcgg tcctcggggc ccctgggcgt taacggagcc
      241 gtcccgcttt ctggggcaat cccttcggga tcgctgggtc gcccgggccc cggcgcccgc
      301 gccgtccccg gccgttcccg gtcctgcccc cctgggcgaa gccctgggtc gggccgcggc
      361 ccgggcggag gccgcggact tcgacggccg ggagatcctc gacgccctgg accggctggt
      421 gccggtcctt tcccaaaacg tcgaaaccaa cggggagtcg gcgcccccgg cggacctcgc
      481 cgcccagcgc gacgaattcg tggaggcctt ccgccgccaa tgggccgccg aaaccgaccg
      541 gcccctgtcc ttggccgagg cgcggagcgc cctgacctcc accgttgtgc aatcccggtt
      601 cgaccttccg gcggaaattc cgccggccgg cccggaggtc gcgcgggacc atttggttat
      661 cgttccgctg acgttcacgg cgcgggaatt ggcgtccttt gtggacgcgg ccgtccgggt
      721 ctcccgggga cggcggatcg ttttcgccct gcccgccggc ccgtccttcc agaaaaccaa
      781 agccgaattg ttccgtctgg cggggcgcgc ccagatcgcg ccccacgtcg ccgtcttcgc
      841 cctgccggag ggcgcggtca ccacggaggg cggcccggtt ctccgactgg cggtcgtggc
      901 ccggggtctc ctcggggtcc aaccctccct gatcacggtg ccgcgatcct tggccatcga
      961 cggctccaac ctgcccgagg ggccctaccg gacggccctt ttcctgcttc tcgacgccct
     1021 gctccaggcc gtgcccctgc ggctgggcga cctggacacc atcgaacgga ccgcccgggc
     1081 cgtcgccacc tcggcctaag actgcccggg acagagcttt aaaggattcg tataataagc
     1141 gggtccccgc ggcgatttta atcaagcggg cgccgccctc gcgggcgcgt ccaggtttgg
     1201 gaacggcgga gggggcggat tcgtgtacaa cccattcgga ggccttatga aaaaatggtt
     1261 ggtggttttg atggcggcgg ttttctg
//
LOCUS       DAOQLS010000697         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_91315_length_1286_cov_4.528838, whole genome shotgun sequence.
ACCESSION   DAOQLS010000697 DAOQLS010000000
VERSION     DAOQLS010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91315_length_1286_cov_4.528838"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..536)
                     /gene="bcp"
                     /locus_tag="PK362_10760"
     CDS             complement(84..536)
                     /gene="bcp"
                     /locus_tag="PK362_10760"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNC75348.1"
                     /translation="MLEIGDKAPAFTALDQDGKKVSLSDFKGKQVVLYFYPKDMTPGC
                     TVEACAFRDGHGALQKAGAVVLGVSKDPVPSHKKFSDKFSLPFSLLADEDLKIMKAYG
                     AWGEKSLYGRKFMGTLRVTYIINSNGKIKAVFKKVKPAGHDREILALL"
     gene            complement(520..942)
                     /locus_tag="PK362_10765"
     CDS             complement(520..942)
                     /locus_tag="PK362_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNC75349.1"
                     /translation="MEWIFSTEPKEYPWSKVQEGAARWDGIRGPAARKYMREIQAGDR
                     IWGYHASPQKALVCWAVAAAPAYPDPAAPDWLAIDLCFDRWLPRPIALAELRADARLA
                     DMPFLRIPRLSVAPVTSDQSKRLFSLCRNPEDSRARDR"
     gene            complement(942..>1286)
                     /locus_tag="PK362_10770"
     CDS             complement(942..>1286)
                     /locus_tag="PK362_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNC75350.1"
                     /translation="ALEGRLPVLPPFEPLAESEMADILEELRERWPGADALARHRLGD
                     VRVGETGMLLVVTAPSSSEAFQACHYALDQMKTRIPLWKKDRYADGSGEWSTQHRETM
                     LISDSVLTIPQS"
BASE COUNT          259 a          382 c          384 g          261 t
ORIGIN      
        1 ttgggcttca tcgagcgtga aataacgctg atgcataaga ataccttact aaattgtagg
       61 ccttttgtcc agccctattt cactcacaaa agggccagga tttcccggtc gtgtccggcg
      121 ggtttgactt ttttaaaaac cgcctttatt ttaccgttcg aattgataat gtaggtcacc
      181 cgaagggtgc ccatgaattt ccggccgtac agggactttt ccccccaggc gccgtaggcc
      241 ttcatgattt tcaaatcctc gtccgccagg agcgaaaagg gcagggagaa tttgtcggag
      301 aatttcttgt ggctcggaac cgggtccttg ctcaccccca ggaccacggc accggctttt
      361 tgaagagcgc cgtgtccgtc gcggaaagcg caggcctcca cggtgcatcc cggggtcatg
      421 tccttggggt aaaaatagag gaccacttgc ttccccttga agtccgacag ggaaactttc
      481 tttccgtcct ggtccagggc ggtaaaagcg ggggccttgt caccgatctc gagcacggga
      541 atcctccgga tttcgacata acgaaaaaag tcgtttggac tggtccgacg tgaccggcgc
      601 caccgacagt cgcgggattc gaaggaaagg catgtcggcc agtcgggcgt cggcgcgcaa
      661 ttcggccagg gcgatcggcc gcggcagcca ccggtcaaaa cacagatcga tggccagcca
      721 gtcgggggcc gccggatcgg ggtacgcggg cgcggcggcc acggcccaac acaccagcgc
      781 cttctgaggc gaggcgtgat aaccccaaat tcgatcgccg gcctgaatct cccgcatata
      841 tttccgggcc gcgggacccc gaatgccgtc ccaacgggcc gcgccctcct gcaccttcga
      901 ccaaggatat tccttgggtt ccgtcgaaaa aatccactcc atcagctttg gggaatggtc
      961 agaacgctgt cggaaatcaa catggtttcg cggtgttggg tggaccattc gcccgatccg
     1021 tcggcatagc ggtctttttt ccacaagggg atccgggttt tcatttggtc cagggcgtag
     1081 tggcaggcct ggaaggcctc ggaactggaa ggggcggtga ccaccaaaag cattccggtt
     1141 tcccccacgc gcacgtcgcc caatcggtgg cgcgccaggg cgtcggcccc gggccaccgt
     1201 tcccgaagtt cctccaggat atcggccatt tccgactcgg ccaggggctc gaagggcggc
     1261 aagacgggca aacggccttc gagggc
//
LOCUS       DAOQLS010000698         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_91394_length_1286_cov_2.258327, whole genome shotgun sequence.
ACCESSION   DAOQLS010000698 DAOQLS010000000
VERSION     DAOQLS010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91394_length_1286_cov_2.258327"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK362_10775"
     CDS             <1..296
                     /locus_tag="PK362_10775"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75351.1"
                     /translation="SLTDYLSAIESGAGPVADVVELGAAERRGEDLMLGLRLREGVRP
                     SPEALADYGPVLERFAALGFLTRSGEAYLPTRRGWILSNQLFEHLLSPVGSPP"
     gene            361..>1286
                     /locus_tag="PK362_10780"
     CDS             361..>1286
                     /locus_tag="PK362_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75352.1"
                     /translation="MGPRINPSRSATSWRRFLGAFLTAVYFVQSVLLAAAPESNFWSE
                     RRRSQDAQRRTLAMVSLPGPSVDAARVTAAWPTPAPEPAPVHFSVPPALRQRVARVLE
                     ALPAENITVRGLVVPPGPPNAVLVHLQDVHLNPEAQSNLGKTLASLAAHRLGDLVALE
                     GVFDAVDLAAYRQRPERDAVQTSADYFLRTNKISGPVQALMTLAEPAPVLVGVDDKAR
                     YDANVAAYLRSAPIRPGLQKAIDGWKAETAARKGQTFSPALSRFDAVVQGRRDRRTGL
                     GEYARAVSDAAPPELPRTAVRAFLQAFAMEKSL"
BASE COUNT          195 a          460 c          388 g          243 t
ORIGIN      
        1 aatcgttgac ggattatctc tccgcgatcg aatccggcgc ggggcccgtg gcggacgtcg
       61 ttgaattggg cgcggccgag cgccgcgggg aggacctgat gctgggcctc cggctccggg
      121 agggcgttcg cccgtcgccc gaggccctcg cggactacgg tcccgttctg gagcggtttg
      181 cggccctcgg atttttaacc cgctcgggcg aggcctatct ccccacccgc cggggttgga
      241 ttctttccaa ccaactgttt gagcacctct taagccccgt cggttccccg ccctaacatt
      301 tacaaaaagg ttacattaat ttaaggttct tctcgtcctt cctcgtttaa attagaggat
      361 atgggacccc gcatcaatcc ttcccgttcg gcgacctcct ggcgtcgatt tctcggcgcg
      421 tttttaaccg ccgtttactt cgtccaaagc gtccttttgg ccgcggcgcc ggaatccaat
      481 ttttggtcgg agcgccgccg ctcccaggac gcccaacggc gcaccctggc gatggtttcc
      541 ctgcccggac cgtcggtgga cgcggcccgg gtgaccgccg cttggccgac gcccgccccc
      601 gaaccggccc ccgtccattt ctccgttccc ccggcccttc gccagcgggt ggctcgggtg
      661 ttggaggccc ttccggcgga aaacatcacc gtgcggggtt tggtggtgcc cccgggaccg
      721 cccaacgcgg tgttggtgca cctgcaggac gtgcatttga accccgaagc ccaatcgaat
      781 ctcgggaaaa cgctcgcttc cctggccgcc caccgcctgg gggatttggt ggccttggag
      841 ggagtgttcg acgccgtcga cctggcggcc taccgccaac ggcccgagcg ggacgccgtt
      901 caaacctcgg cggattactt ccttcgaacc aacaaaatct ccggccccgt ccaggccctg
      961 atgaccctcg ccgaaccggc tccggtcctg gtcggcgtcg acgacaaggc ccggtacgac
     1021 gccaacgtcg ccgcctacct tcgatcggcc ccgatccgcc ccggcctaca aaaagccatc
     1081 gacggctgga aagccgagac ggccgcccgg aaaggccaaa cgttttcgcc cgccttgtcc
     1141 cgcttcgacg cggtggtgca gggacggcgc gaccgccgga ccggcctggg ggaatacgcc
     1201 cgggcggtga gcgacgccgc cccgccggaa ttgccccgaa ccgccgtccg ggcttttctc
     1261 caggccttcg ccatggaaaa aagcct
//
LOCUS       DAOQLS010000699         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_91593_length_1284_cov_4.215622, whole genome shotgun sequence.
ACCESSION   DAOQLS010000699 DAOQLS010000000
VERSION     DAOQLS010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91593_length_1284_cov_4.215622"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK362_10785"
     CDS             complement(<1..428)
                     /locus_tag="PK362_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNC75353.1"
                     /translation="MTLAWPRSLVGELKTWAEKCYPYEGCGLLIGRQEKADWRQAERF
                     FPLKNLLRHRNEADPGTIDTAAGTLGARVHTQGQFEFVIDPAEFNRAVLDAARSGLDV
                     VGVLHTHPDHPAKPSPTDAAQPLLAGWSNVIVKVDQAQFV"
     gene            complement(425..>1284)
                     /locus_tag="PK362_10790"
     CDS             complement(425..>1284)
                     /locus_tag="PK362_10790"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNC75354.1"
                     /translation="EVYAKAEFTNPGGSVKDRAAKNMILEAIKSGELTKDKILLDSTS
                     GNTGIAYAMIGAALGYRVELVMPENASEKKRIIEAFGAKVIYTDPLEGSDGAQREAQR
                     LYEADPKRFYLPDQYNNPNNWKAHYKTTAEEIWRQTEGRVTHFVAGIGTSGTLMGTGR
                     RLKERNPNIRVIAVEPSTPLHGLEGLKHMETSIVPGIYDPRVHDRKVSVYTEDAYEMC
                     CRMAREEGVLVGYSCGAALQGVFEVAMGLEEGVLVTVLPDSGERYLHTRYWEELLDNF
                     EDYMKDHEL"
BASE COUNT          215 a          378 c          402 g          289 t
ORIGIN      
        1 tccacaaatt gggcttgatc caccttcacg atgacgttgg accacccggc cagaagcggt
       61 tgggcggcgt cggtgggact gggtttcgcg gggtggtcgg gatgggtgtg cagcacgccg
      121 accacgtcca accccgaccg ggcggcgtcc agaacggccc ggttgaattc cgcgggatcg
      181 atcacaaact cgaattgccc ttgggtgtgg acccgggcgc ccagggttcc ggcggcggtg
      241 tcgatcgttc cggggtccgc ttcgttccgg tgtcggagga ggttttttaa agggaaaaaa
      301 cgctcggcct gccgccagtc ggctttttcc tgacggccga tcagcaaccc gcatccctcg
      361 taagggtagc atttttccgc ccaggtcttg agttccccca ccagggaacg gggccaggcc
      421 agggtcacag ttcgtggtcc ttcatgtaat cctcgaaatt gtccaggagc tcctcccaat
      481 agcgtgtgtg aagatatcgt tcgccggaat cgggcaggac ggtcaccagg accccttctt
      541 ccaaacccat ggccacctcg aaaacgccct gaagggccgc tccgcaggaa tagccgacca
      601 aaacgccctc ttcccgggcc atgcggcaac acatctcgta ggcgtcttcg gtgtagaccg
      661 acaccttccg gtcgtgaacg cgggggtcgt aaatgcccgg aacgatggag gtttccatgt
      721 gttttagccc ctccaggccg tgcaacgggg tggagggttc cacggcgatc actcgaatgt
      781 tcgggttccg ttccttgagc cggcggccgg ttcccatgag ggtgccgctg gtgccgatcc
      841 ccgccacaaa atgggtcacg cggccttccg tttgacgcca aatttcctcg gccgtcgttt
      901 tgtaatgggc tttccagttg ttggggttgt tgtattggtc gggaaggtag aagcgcttgg
      961 gatcggcttc gtagaggcgc tgggcttcac gctgggcgcc gtcggaacct tcgagggggt
     1021 ccgtgtaaat gaccttggcg ccgaaggctt cgatgatccg ctttttttcc gaggcgtttt
     1081 cgggcatgac caattccacc cggtaaccga gggccgcgcc gatcatggcg taggcgatcc
     1141 cggtattccc ggaggtcgag tccagcagga ttttgtcctt ggtcaattcc ccgctcttga
     1201 tcgcttctaa aatcatgttt ttggcggccc ggtccttgac cgatccgccg gggttggtga
     1261 attccgcttt ggcgtagacc tcca
//
LOCUS       DAOQLS010000700         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_91938_length_1282_cov_2.814181, whole genome shotgun sequence.
ACCESSION   DAOQLS010000700 DAOQLS010000000
VERSION     DAOQLS010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91938_length_1282_cov_2.814181"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PK362_10795"
     CDS             complement(<1..583)
                     /locus_tag="PK362_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNC75355.1"
                     /translation="MDDITSVLKEKHVFRPSKSFSEKARVSSFAQYEKLYRRSLKNPE
                     AYWAAAAKELHWFKPWKKTLDWKLPFAKWFVGGKTNVAFNCVDRHLSGWRRNKAAFVW
                     EGEDGEERVLTYQDLHREVGRFSNALKSLGVAKGDRVCLYMPLVPELAIAMLSCARIG
                     ATHSVVFGGFSSQAIRDRVNDAGCKVVVTADGGY"
     gene            complement(632..1186)
                     /locus_tag="PK362_10800"
     CDS             complement(632..1186)
                     /locus_tag="PK362_10800"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNC75356.1"
                     /translation="MRSDALFADVPLAEWARLINHGPCVIVTSGDAARANAAPAQWNM
                     PVNDDPPVAALALDADNFTAALVAKTGEFVLNVPDAALLPALKILGSRSGRDGDKIGP
                     TGLTLAPGRKVAVPHLAAALAFIECKVRKTVDVDGVRLILGDVLYAGASPAHFKDNRW
                     LPGVQTVHHLGGMVFALMGERRSP"
BASE COUNT          253 a          380 c          400 g          249 t
ORIGIN      
        1 ggtacccgcc gtcggcggtc acgaccactt tgcacccggc gtcgttcacc cggtcgcgga
       61 tcgcctggga ggagaacccg ccgaaaacca cggagtgggt cgccccgatg cgcgcgcagg
      121 aaagcatggc gatggccagt tcgggcacca ggggcatgta gaggcacacc cggtcgccct
      181 tcgcgacccc cagggatttg agggcgttgg aaaaacggcc cacctcccgg tgaagatcct
      241 ggtaggtcag gacccgttct tccccgtcct cgccctccca aacgaacgcg gccttgttcc
      301 gccgccagcc cgacagatgc cggtcgacgc agttaaaagc gacgttggtc tttcccccca
      361 cgaaccactt cgcgaagggc aatttccaat ccagtgtttt cttccagggt ttaaaccaat
      421 ggagttcctt cgccgccgcc gcccaatagg cttcggggtt tttcaagctt cgacggtaaa
      481 gtttttcgta ttgggcgaag gaggatacgc gggctttttc ggaaaacgac ttggagggac
      541 ggaacacgtg cttttctttg agtacggagg taatgtcgtc catggcacac cccttaaaag
      601 gattgcgttc ggctgagttt accaaaaagg ctcagggaga gcggcgttcc cccatgaggg
      661 cgaacaccat gcccccaagg tgatgaacgg tttggacgcc cggaagccag cggttgtcct
      721 tgaaatgggc cggagaagcc ccggcgtaga ggacgtcccc caggatcaac cggacgccgt
      781 ccacgtccac cgttttccga actttgcatt caatgaatgc aagggccgcg gcgagatgag
      841 gcacggcgac cttccgaccc ggcgcgaggg tcaggcccgt gggcccgatt ttgtccccgt
      901 cgcgccccga acgcgatccc agaattttta aggcggggag gagggcggcg tcgggaacgt
      961 tcaacacgaa ttccccggtt ttcgcaacga gcgcggcggt gaagttgtcg gcgtcgagcg
     1021 ccagggcggc gacgggcggg tcgtcgttca cgggcatgtt ccactgggcg ggggccgcgt
     1081 tggcccgcgc ggcgtcgccc gaggtcacaa tgacgcaagg cccgtgattg atcaatcggg
     1141 cccattcggc gagcggaaca tccgcaaaaa gcgcgtcgga tctcatttta tttaaaatga
     1201 atgctgttca atgttttatc gtgtttttga gccattgaaa ccggcgtgaa ttgcgcctat
     1261 ccttaagcga gggagcccat gc
//
LOCUS       DAOQLS010000701         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_92106_length_1281_cov_2.572594, whole genome shotgun sequence.
ACCESSION   DAOQLS010000701 DAOQLS010000000
VERSION     DAOQLS010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92106_length_1281_cov_2.572594"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..>1281)
                     /locus_tag="PK362_10805"
     CDS             complement(36..>1281)
                     /locus_tag="PK362_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75357.1"
                     /translation="SWLFLYWRNDFGPKKADLFFPFYYRERGPRRWTVATPVFWSRRS
                     SLSREGALPLAYWYASREKARTFVLPLYGHARTPDSRWTVVGLGYHLQNAEGRTYGLF
                     PLWGRFFSEKSSGSYLLPFYWSSGDGRGNGSLVLPLLLTSVARSKTGTPDRERHVNYL
                     ILGSVHQKGPDLSHGFFPLYQYVREGDFRNFWAPRILPLAAWERRGDSRLGYLFPYGG
                     RRTPALVWDFIFPLWYRSCHYAVEEGSVPRRGEVTERASAFFPVYWSGSNATRSYAFV
                     PPIYGRFDESVFTETGEHRRRFRVVTPLGWSADTAAGGKFRLFFPVYWRFVTAPRADH
                     STKDVAVYGPWFRVKTAGAVGGSRTIGLAPFFSATRTTEGDRYFDVLGGLYARDARGG
                     RVRHRFLYFFSTAPRWNTGGQK"
BASE COUNT          284 a          400 c          403 g          194 t
ORIGIN      
        1 atggagttct ccttgtgtgg ggtggttcta tttaactatt tttgcccgcc ggtgttccac
       61 cggggagccg ttgagaaaaa gtaaagaaac cggtggcgaa cccgcccgcc ccgggcgtct
      121 cgggcgtaca ggccccccag aacatcgaaa taccgatccc cctcggtcgt tcgggtggcc
      181 gaaaaaaacg gcgccaagcc gatcgtccgg gatcccccca cggccccggc cgttttcacg
      241 cgaaaccaag gcccgtaaac cgccacgtcc ttcgtggagt gatccgcccg gggggcggtg
      301 acgaagcgcc aatagaccgg gaagaaaagg cggaattttc cgccggcggc cgtgtcggcg
      361 ctccaaccca ggggcgtgac cacccggaac cgccgtcgat gttctccggt ttcggtgaac
      421 acggactcat cgaaacggcc gtaaatcggg ggcacaaacg cgtaggagcg ggtggcgttg
      481 ctcccgctcc aatacaccgg gaagaacgcg ctggcccgtt ccgtgacctc cccccgccgg
      541 gggaccgagc cttcctccac cgcgtaatgg caagaacggt accaaagggg aaaaataaaa
      601 tcccaaacca gggcgggcgt ccgccgtccc ccgtagggga agaggtaacc gagacgggaa
      661 tccccccgtc gttcccaggc cgcgagcggc aggatccggg gcgcccaaaa gtttcgaaaa
      721 tccccctccc ggacgtattg gtagagcgga aagaacccgt gggacagatc cggccctttt
      781 tgatgcacgg agcccaaaat caaatagttg acatggcgtt cccggtccgg ggtaccggtt
      841 ttgctccggg cgaccgaggt gagaagcaag ggaagcacca gggaaccgtt gccccggccg
      901 tccccggaag accaatagaa gggcagaagg taacttcccg aggatttttc ggaaaagaaa
      961 cggccccaca gggggaacag gccgtaggtc cgcccttcgg cgttttgaag gtggtagccc
     1021 aatcccacga cggtccagcg gctgtccggc gtacgggcgt ggccgtagag gggcaaaacg
     1081 aaggtccggg ccttttcccg actggcgtac caataggcca ggggcagggc gccctcccgg
     1141 gacaaggagc tccgccggga ccaaaacacc ggcgtggcca cggtccaccg acggggcccc
     1201 cgctcccggt aataaaaagg aaaaaacaga tcggctttct tgggtccgaa atcgtttcgc
     1261 cagtagagga acaaccagct c
//
LOCUS       DAOQLS010000702         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_92497_length_1278_cov_3.194603, whole genome shotgun sequence.
ACCESSION   DAOQLS010000702 DAOQLS010000000
VERSION     DAOQLS010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92497_length_1278_cov_3.194603"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /gene="rpe"
                     /locus_tag="PK362_10810"
     CDS             <1..598
                     /gene="rpe"
                     /locus_tag="PK362_10810"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNC75358.1"
                     /translation="AAGADWIHVDVMDGHFVPNLTIGPVVVHWLKKCTDLPLDVHLMI
                     ENPEKYIPAFAKAGAWNITVHREACRHPAEVLRLIKKHGCRAGMSVRPRTPIRAVEPH
                     LKNLDVVLVMTVEPGFGGQSFMPDMLPKVRWLRDHWRGKRKEGPWIEVDGGINADTAP
                     LTVHAGANVLVAGNAIFGAPNPGRALRDIRRRAAAPVL"
     gene            595..>1278
                     /gene="bamD"
                     /locus_tag="PK362_10815"
     CDS             595..>1278
                     /gene="bamD"
                     /locus_tag="PK362_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNC75359.1"
                     /translation="MTLPLRLSSSPRAVFTPRWERLGAVLIFFVLGCGPSEPHPRATA
                     TLTAEERDSASFIQEESREWYLWHNHGNRVQREGKFDEALDSYNRAQKAWPKSGESTD
                     TALQKAYLYLRLRQPAWAVRYADRFAAHYPRDSNPPYVRAFAYYDLGDTAKALEQADR
                     LGPENMARRMVHGLVQMKAGDRAGGTAAVVAAWTDYLKRHHRYPAPPRPEDMIQIKFL
                     NWAVLPLDAH"
BASE COUNT          230 a          420 c          409 g          219 t
ORIGIN      
        1 ggccgccggc gccgactgga tccacgtcga cgtcatggac ggccacttcg tgcccaattt
       61 gaccatcggc ccggtggtcg tgcattggct gaaaaaatgc acggaccttc ccctggacgt
      121 ccatctgatg atcgaaaacc cggaaaaata catcccggcc ttcgccaaag cgggggcctg
      181 gaacatcacg gtgcatcggg aggcctgccg ccaccccgcg gaggtgttgc ggctcatcaa
      241 aaaacacggg tgccgggcgg gaatgtccgt ccgaccccgg acgcccatcc gggcggtcga
      301 gccccatttg aaaaatttgg acgtcgtgct ggtcatgacc gtggagccgg gattcggcgg
      361 ccaatccttc atgccggaca tgctgcccaa agtccgctgg cttcgggacc attggcgcgg
      421 gaaacggaaa gagggcccct ggatcgaagt ggacggcggc atcaacgccg acaccgcgcc
      481 cctgacggtc cacgcgggcg ccaacgtgct cgtggcgggg aacgccattt tcggcgcccc
      541 gaaccctggc cgggcccttc gggacatccg ccgtcgggcc gccgcgccgg tcctttgacc
      601 ctgcccctcc gcctttcttc ttcgccccgc gccgttttca cgccccgttg ggaacgcctc
      661 ggggcggtcc tgattttttt cgtcctgggg tgcggcccgt cggaacccca tccccgggcg
      721 acggcgaccc tgaccgcgga agagcgcgat tccgcgtcct tcattcaaga agaatccagg
      781 gaatggtatc tctggcacaa tcacggaaac cgggttcagc gggaggggaa attcgacgag
      841 gcgttggatt cgtacaaccg ggcccaaaag gcctggccga aatccgggga atccaccgac
      901 acggcccttc aaaaggccta tttgtacctc cgcctgcggc agccggcctg ggccgtacgt
      961 tacgccgatc ggttcgccgc ccattatccc cgggacagca acccgcctta cgtgcgggcc
     1021 ttcgcctact acgacctggg cgacacggca aaggccctcg agcaggccga tcggttgggg
     1081 ccggagaaca tggcccggcg gatggtccat ggattggttc agatgaaggc gggggatcgg
     1141 gcggggggaa cggccgcggt ggttgcggcg tggacggact atttaaagcg acatcaccgc
     1201 tacccggcgc cgccccggcc ggaggacatg attcaaatca aatttttgaa ttgggcggtc
     1261 ctgcccttgg acgcccat
//
LOCUS       DAOQLS010000703         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_92733_length_1276_cov_4.041769, whole genome shotgun sequence.
ACCESSION   DAOQLS010000703 DAOQLS010000000
VERSION     DAOQLS010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92733_length_1276_cov_4.041769"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK362_10820"
     CDS             complement(<1..598)
                     /locus_tag="PK362_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75360.1"
                     /translation="MSLFSVLLRDPRGAEFPLVSKILGDFLKVPVIDAQHKARDAWGF
                     LGEKLSAEDAQRLAAAAQAAGAPARVVPESDLAVSPPAQSANGGGPENDVLRLTLGVP
                     AREKRIPLADLQLIALLPLREESISTVTVKEGPSAGERAAKITLMMTTGIPLPMGKSK
                     EIKKTVTTGDLTVLADFFTATERFRVDAAAEVHELMRAD"
     gene            complement(612..>1276)
                     /locus_tag="PK362_10825"
     CDS             complement(612..>1276)
                     /locus_tag="PK362_10825"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC75361.1"
                     /translation="ALGQVRTPAALQFLLRAHAAEENPKARRPINAALGEFREAAVRE
                     RLWETLAQEKSYFAETQALKSLAKQGDATVERLIDEALGRESWNDVLRSGALEALTLL
                     RSPKAVDVLIRHTAYGVPHTRRMTAIRCLAQLGGGREDVLRRLTALLDDPYLLVQMSA
                     VRALGQVGDERAVPALEKLTKGDWDGRLIRTAEETVLKLKKNIDDSPKPNRRGGGARR
                     RS"
BASE COUNT          196 a          414 c          443 g          223 t
ORIGIN      
        1 cgtcggcacg catgagttcg tggacttctg ccgccgcgtc aacgcggaac cgctcggtgg
       61 cggtgaaaaa atcggccagg acggtcaggt ccccggtggt cacggttttc ttgatctctt
      121 tggattttcc catggggagg ggaatgccgg tggtcatcat caaggtgatt ttggccgcgc
      181 gctcccccgc cgagggacct tccttgaccg tcacggtgga gattgattcc tcccgcaggg
      241 gcaaaagggc gatcaattgg aggtccgcca ggggaattcg tttttcccgg gcgggaaccc
      301 ccagggtgag gcgaaggacg tcgttttcgg gcccgccccc gttggccgat tgggccgggg
      361 gcgaaacggc cagatccgac tcgggaacga cccgggcggg ggcgccggcc gcctgggcgg
      421 cggccgccag acgctgggcg tcctcggcgg acaatttctc cccgaggaag ccccaggcgt
      481 cccgggcttt gtgttgggcg tcgatgacgg ggaccttgag aaagtccccc agaattttgg
      541 acaccagcgg aaactccgcc ccccggggat cccgcaggag aacggaaaag aggctcacgc
      601 ccgtcccggg attacgaccg tcgacgcgcc ccgccgcccc gtcgattggg tttgggcgag
      661 tcgtcgatgt tcttcttgag cttcagaacg gtttcctcgg cggtgcggat caaacgcccg
      721 tcccaatcgc ccttcgtgag tttctccagg gcgggcaccg cgcgttcgtc gcccacctgg
      781 cccagggccc ggacggcgga catctggaca agcaggtagg gatcgtcgag caacgccgtc
      841 agccgccgca aaacgtcttc ccgccccccg cccagttggg ccaggcatcg gatggccgtc
      901 atccggcggg tgtgcggcac gccgtaggcg gtgtgccgga tcaacacgtc caccgccttc
      961 ggggaccgga gcagggtcaa ggcctccagg gcgccggaac ggaggacgtc gttccaggat
     1021 tcccgtccca gggcttcgtc gatgagccgc tccaccgtgg cgtcgccctg tttggccagg
     1081 gacttgaggg cctgcgtctc ggcgaaatag cttttctcct gggccagggt ttcccaaagc
     1141 cgctcgcgca cggcggcctc ccggaattcg cccaacgccg cgttgatggg acggcgggcc
     1201 ttgggatttt cctcggcggc gtgggcccgc aacaggaatt gaagggcggc cggcgtgcgg
     1261 acctggccca gggcct
//
LOCUS       DAOQLS010000704         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_92884_length_1275_cov_3.433607, whole genome shotgun sequence.
ACCESSION   DAOQLS010000704 DAOQLS010000000
VERSION     DAOQLS010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92884_length_1275_cov_3.433607"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PK362_10830"
     CDS             complement(<1..456)
                     /locus_tag="PK362_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009634532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNC75362.1"
                     /translation="MRATVAPAVKGKWEVREIPRPDIGPNQVLIQIKASGLCYTDVHI
                     TNGALPTKFPRVLGHEPVGEIVAVGSAVTTRRVGDRVGVPWVQSTCGRCEWCLRGKGL
                     FCPEQIGTGVESPGSHAEFMPAFAQATVLIPDGVAYEQAAPIFCASYTVW"
     gene            complement(515..>1275)
                     /locus_tag="PK362_10835"
     CDS             complement(515..>1275)
                     /locus_tag="PK362_10835"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNC75363.1"
                     /translation="RRGESIPLAEKTFVLSRALPTREAERAFFGDFISRLGFDVDTTF
                     ARRFEGEADLFQAGDVHLFTSGPLEHQRFTPRWGWPPWRRVYGFRSDREALDLLTPYA
                     GTPVIPLTLVRETHYHGDTCLCAFGPRREYLMAYLPALSPESGDRLRERFRERLIPLG
                     PEDGEGFSANSFQLDGDRPVLFMPERSTAALRAAVRERGVDVVPVDVSEFLEKGGGSV
                     KCMVADLGPLPAEAPPAVQTFRRERLFRPAERAQ"
BASE COUNT          230 a          417 c          428 g          200 t
ORIGIN      
        1 ccacacggtg tagctggcgc aaaaaatcgg ggcggcttgc tcgtacgcca cgccgtcggg
       61 gatcaacacc gtggcctggg cgaaggccgg catgaactcc gcgtggctcc cggggctctc
      121 gaccccggtt ccgatctgtt ccgggcaaaa aaggcccttc ccccgcagac accattcgca
      181 acggccgcag gtcgattgca cccagggcac gcccacccgg tcccccaccc gacgcgtggt
      241 cacggcggag ccgacggcga cgatctcccc caccggctcg tggcccagca cccgggggaa
      301 tttcgtcggc agggcgccgt tggtgatgtg gacgtcggtg tagcagaggc cgctggcttt
      361 gatttggatc aagacttggt tgggaccgat gtcgggccgg gggatctccc ggacttccca
      421 ttttcctttg acggcggggg cgacggtggc gcgcataaga aaactcctgg attgagggag
      481 aagaagactt attttatctt ttttgggggt tggcttattg ggcccgttcg gcgggacgaa
      541 acaaccgttc ccgacggaaa gtttggacgg cgggcggcgc ctcggcgggg aggggaccca
      601 aatcggccac catgcatttg acggacccgc cgcccttttc caaaaactcg gagacgtcca
      661 cggggaccac gtccaccccc cgctcccgca cggcggcccg aagagcggcc gtggaccgtt
      721 cgggcatgaa cagaacgggc cggtccccgt cgagctgaaa actgttggcc gaaaacccct
      781 ccccgtcctc gggccccagg gggatcagcc gctcccggaa tcgttcccgc aaccggtccc
      841 cgctttccgg ggagagcgcc ggcaaatagg ccatcaggta ttcccgccgg ggaccgaagg
      901 cgcacaggca cgtgtcgccg tggtaatggg tttcacggac cagggtcagg gggatcaccg
      961 gcgtgccggc gtagggcgtc agaaggtcca gggcctcccg gtcgcttcga aagccgtaca
     1021 cccgccgcca gggcggccag ccccaccggg gggtgaatcg ctggtgttcc aggggccccg
     1081 aggtgaacag gtggacgtcg ccggcctgga acaaatcggc ctcgccttcg aaacgccggg
     1141 cgaaggtcgt gtccacgtcg aaacccaaac gcgaaataaa atcgccgaag aaggcacgtt
     1201 cggcttcccg ggtgggcagg gcccggctca gcacgaaggt cttttccgcc aagggaatcg
     1261 actccccccg gcggg
//
LOCUS       DAOQLS010000705         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_92953_length_1275_cov_2.045902, whole genome shotgun sequence.
ACCESSION   DAOQLS010000705 DAOQLS010000000
VERSION     DAOQLS010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92953_length_1275_cov_2.045902"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1275)
                     /locus_tag="PK362_10840"
     CDS             complement(<1..>1275)
                     /locus_tag="PK362_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75364.1"
                     /translation="LRTHSETTGLEENAAGQMIASRERITHPGQTVVADEIVKSEMAY
                     SPLGQLASYREQTLSTGPGLSMRADAQKTLSYDGAGRVIAATEAGARNGSAYTANTFH
                     VGFDLKGRPVHSVEQSTGPGGPSNIERSAVAFDDFGRRVSYHEEGVSGGSMVHQTVFA
                     EYDALGREAATEATGFRESGAFRERSESTGFNDLGQSTGRRVTGHSAAEGDYAYSQTG
                     QIYGLAGDLISFDKERTDADGITRSHWTSKGTDARGRSLGYTESGSVAKDGRPLYEFT
                     TEHTAGAYTLQNQSSSYDAVTTKRYADGAVETAATRWTDGAYDAQGRLTGFKETQTTT
                     LTDNGETKSSTSSRLRESATYYDDNGPGHLKGQLASTVDVLLDDLSPEKARRVRTADM
                     TYDAEGRSIGAETTTGEVNRLKDALRRLSELWG"
BASE COUNT          187 a          383 c          441 g          264 t
ORIGIN      
        1 ccccatagtt cgctcagccg ccggagggcg tcctttaacc ggtttacctc gccggtcgtg
       61 gtttcggcgc caatcgaacg gccttcggcg tcgtaggtca tgtcggcggt tcggacccga
      121 cgggcctttt cgggcgataa atcgtccagc aggacatcga ccgtggaggc caattggcct
      181 ttgagatggc cgggcccgtt gtcgtcgtag tacgtggcgc tttcccgaag gcgggagctc
      241 gtcgacgatt tcgtctcgcc gttgtccgtc aacgtggtcg tttgggtttc cttaaagccg
      301 gtcaggcggc cctgggcgtc gtaagccccg tcggtccaac gggtggcggc cgtttccacc
      361 gcgccgtcgg cataccgctt ggtggtgacg gcgtcgtagg aggacgattg gttttgaagg
      421 gtgtaggccc cggccgtgtg ctcggtggtg aactcgtaaa ggggccggcc gtctttggcg
      481 accgatccgg attccgtata ccccagggag cggccccggg catcggtgcc cttggaggtc
      541 caatgggagc gggtgattcc gtccgcgtcg gtgcgctctt tgtcaaaaga gattaaatcc
      601 ccggccagtc cgtaaatctg tccggtttgg gaataggcgt aatcgccttc ggcggcggaa
      661 tgccccgtga cccgccggcc ggtcgactgg cccaaatcgt tgaatcccgt ggattcgctc
      721 cgttcccgga aggcgcccga ttcccggaaa ccggtggcct cggtggccgc ttcccgaccc
      781 agggcgtcgt attccgcaaa gacggtttga tggaccatgg acccgccgga aacgccttcc
      841 tcgtggtagg aaacccgccg tccgaaatca tcgaaggcga ccgcggaccg ctcgatgttg
      901 gaggggccgc ccgggccggt ggattgctca acggaatgaa ccgggcggcc tttcaggtcg
      961 aagccgacat ggaaggtgtt ggcggtgtag gccgagccgt ttcgcgcgcc cgcttcggtg
     1021 gcggcgatga cccggcccgc cccgtcgtag ctcagggttt tctgggcgtc ggccctcatg
     1081 gaaagcccgg gcccggtgga aagggtttgc tcccgatagg aagccaattg ccccaggggg
     1141 gaataggcca tttcggattt gacgatttcg tccgcgacga cggtttggcc agggtgggtg
     1201 atgcgctccc ggctggcgat catttgcccg gcggcgttct cctccagccc cgtggtctcg
     1261 ctgtgggtgc gaagg
//
LOCUS       DAOQLS010000706         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_93343_length_1272_cov_2.714873, whole genome shotgun sequence.
ACCESSION   DAOQLS010000706 DAOQLS010000000
VERSION     DAOQLS010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93343_length_1272_cov_2.714873"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            506..715
                     /locus_tag="PK362_10845"
                     /pseudo
     CDS             506..715
                     /locus_tag="PK362_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            1062..1265
                     /locus_tag="PK362_10850"
     CDS             1062..1265
                     /locus_tag="PK362_10850"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HNC75365.1"
                     /translation="MSERKRISAPQAARALGLSDRMVRLLLAGWVREGILTVVNHSNR
                     KRAYGLSAKYRQFIGNERGSSPT"
BASE COUNT          302 a          316 c          380 g          274 t
ORIGIN      
        1 gtataccctg tgaacggtga ccacgacttc ccgccattta gagagattgg atgtcgctat
       61 tttgggctta acatcacccc gaaactacaa tgattttagc cagctggagg aaaattaata
      121 attcatgacc accagtaata tgaaaaaaaa agtcgtttgg tgtccaaaat attccctcac
      181 tcccaaggcc gggacggcgc ttatggagat cgaggccgcc aagactttgg tggaatcgtc
      241 tcctcttcct ttggccgcgg cggaggaact tcgtcgtcga gcgcgactgc ggtcaaccca
      301 ctattccacg cggatagaag gcaaccgatt gaccctggcc gaagcggaaa aggtgatcga
      361 aggccgacga gtgattttca aggggcgaga gcgggacgtc aacgaggtaa ggaactattg
      421 ggaggctctt ttgcgggggg aacgttgggc ggcagaggga aaacctttta cggaagaatt
      481 aatccggcag attcatgcct tgggtatgga cggcccccga gcgaagccca cgccttttcg
      541 tgacggccag aacgcggtca gggaggcggg ctctggcgcg ctggtttacc tgccgcccga
      601 agccaaagac gtgccggatc tgatgtcggg gcttgtccgc tggacaagtc agacagaaaa
      661 ggaaggagtg ccagttcccg tgattgccgg attggctcat taccaattcg ttaccattca
      721 cccctattac gatggaaatg ggcggacggc gcgtttgctg gcgacgttcc tcctgcatcg
      781 aggtgggtat ggactggacg ggtatctctc actggaagaa cgtcacgcac aagatctgga
      841 gagttattac ggtcgtctgt cgacggaccc tcaccataac tactacatgg gacgagagca
      901 cgctgacctg acgcagtggg ttgaatattt cctcgtcgtc gtggccgggg tttttaagat
      961 cgccaaagat gaaaccattg cattctttaa gaagaggcaa gatcatggtg gccccacggt
     1021 gagggaattg gacccccgag tccgcgtcgt gctggggcat tttgtcagag cggaaaagga
     1081 tttccgcgcc ccaagcggca agagccctgg ggttatcgga ccggatggtc cgccttcttt
     1141 tggcggggtg ggtccgcgaa gggatattga cagttgtgaa ccattcgaac cggaaacggg
     1201 cctacgggtt atcggcaaaa tatcggcaat ttatcggcaa tgaaagagga tcttccccca
     1261 catgacaccc at
//
LOCUS       DAOQLS010000707         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_93457_length_1271_cov_3.563322, whole genome shotgun sequence.
ACCESSION   DAOQLS010000707 DAOQLS010000000
VERSION     DAOQLS010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93457_length_1271_cov_3.563322"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PK362_10855"
     CDS             complement(<1..1252)
                     /locus_tag="PK362_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75366.1"
                     /translation="MTQNLQRLLGRNKMDNLTASLRGTDAFIDALDAVTKESGNQRTE
                     TAQKLAALYAIAGLEATPSTTDGWTKLFGDLRHQTEDVLNASGVSTEEKLDRLQVLGA
                     KLDTIGQITPAGTSEGIRTELNDLKTALAAARQQVTEPAIDSKAQSESKADLDHTNVP
                     ANDVSAVAPTLLAESKENASQTETAVSQNIVNSQGVEANQDQLSLAASQDNTGDAATV
                     GTETPRPDNGRKQRRGYGKKQNVDVEERLVDSVVPGAGETSGETVETPVETLNAVERE
                     LVGDRNAAKTSAVQAGATRFNIFESYLAPLQSGNRAQRALYWAMGGFVMPFVEEAAFR
                     FGVFAEAGQALFGGTGFDLASVAGDTVLFGALFAGLHSVVEMSVIARQNGGGIKGWTS
                     AISDRSVWANFGQRLVFSGAMTAVY"
BASE COUNT          217 a          396 c          379 g          279 t
ORIGIN      
        1 gataaaccgc ggtcatggcg ccggagaaga ccagccgctg gccgaagttg gcccagacgg
       61 agcggtcgga gatcgcggag gtccaccctt tgatcccgcc gccgttttgg cgggcgatga
      121 cgctcatttc gaccaccgag tgaagcccgg cgaagagagc gccgaagagc acggtgtccc
      181 ccgccacgga ggccaaatca aacccggtgc cgccgaagag ggcctgaccg gcctcggcaa
      241 agaccccgaa ccggaaggcg gcttcttcca cgaacggcat cacaaagccg cccatggccc
      301 aataaagggc ccgctgggcg cgattgccgc tttggagcgg cgcgagatag gactcaaaga
      361 tgttgaaacg tgtggccccg gcctggacgg cggaggtttt ggcggcgttc cggtctccca
      421 ccaattcccg ctccacggcg ttcagggttt caaccggcgt ttccaccgtt tccccgcttg
      481 tttcgccggc gccgggcacg acggaatcca cgagccgttc ctccacgtca acgttttgtt
      541 tctttccgta gccccggcgt tgtttccgcc cgttgtcggg acgcggggtt tcggtcccca
      601 ccgtcgcggc gtcgccggtg ttgtcctggg acgctgccaa ggacaactgg tcttgatttg
      661 cctccacccc ttggctgttg acaatgtttt gcgaaacagc cgtttcggtt tgacttgcat
      721 tttctttcga ttcggcaaga agggttgggg ccacagccga aacgtcgttc gccggcacgt
      781 tggtgtgatc caagtcggct ttgctttccg attgcgcctt cgaatcgatc gcgggttcag
      841 tcacttgctg acgagccgcc gcaagggccg ttttgagatc gtttaattcc gtgcgtatcc
      901 cttcgctcgt ccccgcggga gtgatctgtc caatggtgtc gagtttcgcc cccaaaactt
      961 gcaggcgatc gagtttttcc tccgtcgaaa caccggaggc gttcagaacg tcctctgtct
     1021 gatgtcgcaa gtcgccgaac agcttggtcc atccatccgt cgtcgagggg gtcgcttcca
     1081 acccggcgat ggcatacagc gccgcaagct tctgggccgt ttcggttcgc tgattcccgg
     1141 attccttggt gacggcgtcg agggcgtcga taaacgcgtc cgttccgcga agcgaagccg
     1201 taagattatc catcttgtta cgacccaaca accgctgaag gttttgagtc atcgggttcg
     1261 ccttcaatcc c
//
LOCUS       DAOQLS010000708         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_94028_length_1267_cov_4.235974, whole genome shotgun sequence.
ACCESSION   DAOQLS010000708 DAOQLS010000000
VERSION     DAOQLS010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94028_length_1267_cov_4.235974"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /gene="lspA"
                     /locus_tag="PK362_10860"
     CDS             <1..301
                     /gene="lspA"
                     /locus_tag="PK362_10860"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNC75367.1"
                     /translation="PQTNALFVGVTLVILAVLVKLGKELASQGPWSRAALPLVWGGAL
                     GNLMDRLWQGAVTDFLDFHLGGWHWPAFNAADSAITVGIACLVVQHFVPRSLSQG"
     gene            303..1064
                     /locus_tag="PK362_10865"
     CDS             303..1064
                     /locus_tag="PK362_10865"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNC75368.1"
                     /translation="MHPLLFHWKFLSLHTYGLFAALAVLAGHGFTVAWARRRGMDAAA
                     VSDSLPALVVGGLLGGRALYALVHWSEFAGAPLDVLKIWQGGLMWHGGVIGGVSAAAF
                     HFRRRGMPIRAWADLFSPGLALGQALGRVGCFFAGCCYGRACDFPWAVRFTDPASLAP
                     LGLPLHPSQLYDAGWNVLLFLGLWRLARRPFWSPGPGRLAALYLALSALGRWAVELTR
                     GDDRGRLWGSVTVTQGLSLAAAAIVGAWLLRRRNA"
     gene            1061..>1267
                     /locus_tag="PK362_10870"
     CDS             1061..>1267
                     /locus_tag="PK362_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNC75369.1"
                     /translation="MTKEKRFTYKGAPQRLDVFLAKNLESLSRTQIQRLIKENRIDVD
                     GAPAPARHPLAPGQVIVVRLPEFSS"
BASE COUNT          143 a          438 c          432 g          254 t
ORIGIN      
        1 tccccaaacc aacgcgctct tcgtcggggt gaccctggtc atcctcgcgg tcctggtcaa
       61 gctcggcaag gaattggcgt cccaggggcc ctggtcccgg gccgctctgc cgttggtctg
      121 gggcggcgcc ctggggaatt tgatggaccg cctgtggcag ggggccgtga cggatttcct
      181 cgactttcac ctgggcggct ggcattggcc ggccttcaac gcggccgaca gcgccatcac
      241 cgtcggcatc gcctgccttg tcgtgcagca cttcgtcccc cggtcccttt cccagggata
      301 acttgcaccc ccttctcttt cactggaaat tcctgagcct gcacacctac ggcctctttg
      361 cggccctggc ggtcctggcc gggcacgggt tcacggtggc ctgggcccgg cgccggggga
      421 tggacgccgc ggccgtgtcg gacagtctcc cggcgctggt ggtgggcggc ctgttgggcg
      481 gccgcgccct ttacgcgttg gtccattgga gcgaattcgc tggggccccc ctggacgtcc
      541 tcaaaatttg gcagggcggc ctcatgtggc acggcggggt gatcggcggc gtgtcggcgg
      601 ccgcgtttca tttccgacga cggggaatgc ccatccgggc ctgggccgat ttgttttcgc
      661 ccgggttggc cctgggccag gccctgggtc gggtgggttg tttcttcgcg ggctgttgct
      721 acgggcgggc ctgcgacttt ccctgggccg tgcgcttcac cgaccccgcc tccctcgccc
      781 ccctcggcct tcctctccac ccttcccaac tctacgacgc gggatggaac gttcttcttt
      841 ttctggggct ttggcgcctc gcccgacggc ccttttggag cccgggtccg ggacgcctgg
      901 ccgccctcta tttggccctg tccgctctcg gccgatgggc cgtggagctc acgcgcgggg
      961 acgaccgggg gcggttgtgg gggtcggtga ccgtgaccca gggtctgtcc ctcgccgcgg
     1021 ccgcgatcgt cggtgcgtgg ctgttgaggc ggcggaacgc gtgacgaagg aaaagcggtt
     1081 cacctacaag ggcgcgcccc agcggctgga cgtgttcctg gcgaagaatc tggaatccct
     1141 gtcccggacg cagatccaac ggctcatcaa ggaaaaccgc atcgacgtgg acggagcccc
     1201 cgcccccgcg cggcacccgc tggccccggg ccaagtgatc gtggtccggc tgcccgaatt
     1261 ttcctcc
//
LOCUS       DAOQLS010000709         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_94148_length_1266_cov_4.767960, whole genome shotgun sequence.
ACCESSION   DAOQLS010000709 DAOQLS010000000
VERSION     DAOQLS010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94148_length_1266_cov_4.767960"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PK362_10875"
     CDS             complement(<1..1242)
                     /locus_tag="PK362_10875"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC75370.1"
                     /translation="MLQYVVQILQRETGEGKKVGWNLLLVGPPGVGKTTLVRAFSRAL
                     QNRKFEVVPLGGMDDEAELAGHRRTYIGARMGKLARALLDLGISNPIIGLDEIDKLGA
                     RNGDPRAFLLQALDPEQNKDFRDRYLGPAPLDGVIFVATSNSLNIPGPLLDRFTVIHV
                     PGYSVDEKVEIGAHHVLPRLREEMVVSSEIDIARPRALVRAVARGYTWEEGARRLVKL
                     LRKVMLRALREWFDRGATITIDNDSLQRYLGKPWEYPSIRNEDRVGEVAVPTARGQLR
                     FLRVGREAESSETGLIVNQTWGPATVNSARLAGDIVSHRLAGTAAEWGVSLDPGSPAG
                     ALRLDAPGGIGEDDSVKGLAFVVAMMSRRLGLPVRRDAAFAGEVDLRGRVLDVPHVRE
                     RAIAAREAGAAFLFLPSAAEPN"
BASE COUNT          247 a          431 c          364 g          224 t
ORIGIN      
        1 attcggttcg gccgccgaag gcaaaaacaa gaacgccgcc cccgcctcgc gcgcggcaat
       61 cgccctttca cgcacgtgcg ggacgtccaa cacccgcccg cggagatcca cttcgcccgc
      121 aaaagcggcg tcccgccgaa ccgggagtcc caatcgacgg gacatcatgg ccaccacaaa
      181 ggccaaccct ttgacggaat cgtcttcccc gattccaccg ggcgcgtcca accggagggc
      241 gccggccgga gatcccggat ccagggagac gccccattcg gcggccgtcc cggccaaccg
      301 atgggacacg atgtccccgg ccaatcgggc gctgttgacc gtcgccgggc cccaggtttg
      361 attgacgatc aaacccgttt ccgacgattc cgcctcccga cccacccgga gaaaacgcaa
      421 ctgcccccgg gccgtgggaa cggccacctc gccgacccga tcttcgttcc gaatggaagg
      481 gtattcccag ggttttccca gatagcgctg caacgaatcg ttatcgatcg tgatcgtcgc
      541 gccccgatcg aaccattcgc ggagagcgcg caacatcact ttgcgaagca acttgaccaa
      601 tcgacgggcg ccttcttccc aggtgtaacc ccgggccacg gcccgaacca gggctcgcgg
      661 ccgggcgatg tcgatttccg acgagacgac catttcctcc cgaagacggg gcagcacgtg
      721 atgggcgccg atctccactt tttcatccac gctgtagccg ggaacgtgga tcacggtgaa
      781 ccggtccagg agcggtcccg ggatgttgag ggaattggag gtggccacaa aaatgacgcc
      841 gtccaggggc gccggtccca agtagcggtc acgaaaatcc ttgttttgct ccggatccaa
      901 tgcttgaagc aaaaaggccc gggggtcgcc gtttcgggcc cccaacttat cgatttcgtc
      961 caagccgatg atggggttgg aaatgcccaa atccaaaagc gcccgggcca atttgcccat
     1021 gcgcgctccg atgtaggtcc ggcgatgacc cgccaattcg gcttcgtcgt ccatgccccc
     1081 cagggggacg acttcgaatt tgcgattttg cagggcgcgg gaaaaggccc ggaccagggt
     1141 cgtttttccg acgcccggcg gccccaccag caacaggttc catccgactt ttttcccttc
     1201 gcccgtttcc cgctgcagga tttgtacgac gtactggagc atttcggatt tggcttcctc
     1261 caagcc
//
LOCUS       DAOQLS010000710         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_94297_length_1265_cov_3.348760, whole genome shotgun sequence.
ACCESSION   DAOQLS010000710 DAOQLS010000000
VERSION     DAOQLS010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94297_length_1265_cov_3.348760"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1265
                     /locus_tag="PK362_10880"
     CDS             <1..>1265
                     /locus_tag="PK362_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75371.1"
                     /translation="IDGTKRDVKAIDLGNGLWRIAAGAETVSGNTFFARERKLVEGNF
                     FDQVNVTPTIVYEEGGRATTVAFSVEAASPGGKFMLPSVPGQFFRGGAIEYNTMTGQI
                     TSSTGAERLTEIRKDGSTFHVVQKANATGGWASIQVKPGYVLGGDFRPTDGLALFAQN
                     GLAGARMYIDGQEVKIKSSGLVQDGLLQLTFSEGIKDREGKSMTGVKLTSSLEVSSRL
                     YAVTEPTKINGVNVTPTNRLDIDRGPQGTNGGGTPSEGLYPIKGNVPLGSPMYHSLAN
                     GVPGTEGDSPEERAAWGESIATTQLQSGAPNAALALDRSPQLNSGAAIVEVNRAGTVT
                     GVTNAQIDLTALDTTSPNFKGNVRVTSMEGISPLPDADGRFKIESATTLQIRDNAGAW
                     HDVTAAAGGTITITAGGAVFEGYAAANAD"
BASE COUNT          275 a          398 c          377 g          215 t
ORIGIN      
        1 catcgacggt accaaacggg atgtcaaagc catcgatttg ggcaatggac tctggcgcat
       61 cgccgcgggc gcggaaacgg tttccggcaa tacctttttc gcccgggaac gaaagctcgt
      121 tgaaggcaac ttctttgacc aggtcaatgt cacccccacc attgtgtatg aagagggtgg
      181 acgtgcgacc accgtggcct tctccgttga agccgccagt ccgggaggca aattcatgtt
      241 gccttcggtc ccgggccagt ttttccgggg cggcgcgatc gaatacaaca ccatgacggg
      301 acaaatcacc tcttcgacag gggccgagcg gcttaccgaa atccgcaagg atggttcgac
      361 gttccacgtt gtccaaaagg ccaatgccac cggtggatgg gcgtccatcc aggtaaaacc
      421 gggttacgtc ctgggaggcg atttccgtcc caccgacgga ttggccctgt tcgcccaaaa
      481 cggattggcc ggcgcccgca tgtatatcga cgggcaggaa gtgaagatca agtcttcggg
      541 cttggttcaa gacgggctgc tgcaattgac cttctcggaa ggcatcaaag accgcgaagg
      601 gaaatccatg acgggggtca aattgacctc ctcgcttgag gtttcctccc ggttgtacgc
      661 ggtcacggaa ccgacgaaaa tcaacggggt gaacgtcacc cccacgaacc ggttggacat
      721 cgaccgcgga ccccaaggga cgaacggcgg cggaacgccg tccgaagggc tttatccgat
      781 caaggggaac gtgcctctag gcagccccat gtaccattcg ttggccaacg gggttcccgg
      841 aaccgaaggg gattcaccgg aagaacgcgc cgcttggggc gaatccatcg ccacaaccca
      901 gcttcaatcc ggcgccccca acgccgccct ggccttggat cgttcgccgc aattgaacag
      961 cggcgcggcc atcgtggaag tcaatcgcgc gggcaccgtc accggcgtca ccaacgccca
     1021 aatcgacctg acggctttgg acacgacgtc gcccaacttc aaaggcaacg tccgcgtcac
     1081 cagcatggag ggcatttcgc cccttcccga cgccgacggg cgattcaaaa tcgaatcggc
     1141 caccacgctg caaatccggg acaacgccgg cgcctggcac gacgtgaccg cggcggctgg
     1201 cggaaccatc accatcaccg ccggcggggc ggtgtttgaa ggatacgccg cggcgaacgc
     1261 cgact
//
LOCUS       DAOQLS010000711         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_94736_length_1262_cov_3.309031, whole genome shotgun sequence.
ACCESSION   DAOQLS010000711 DAOQLS010000000
VERSION     DAOQLS010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94736_length_1262_cov_3.309031"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PK362_10885"
     CDS             complement(<1..673)
                     /locus_tag="PK362_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75372.1"
                     /translation="MSTSPVPILLVDHDEKDFNAARALLEQFETDTYCLDWAPDYEAG
                     LRALSKGAYALVLVEYHLGGKTGLDFLQSLKDRGPEAPVIVLTEKGDREVDLQVMREG
                     AYDFLIKGEYDASLLERSLRYALSRRKTEEHLLHTARELERMNSTLKETQSRIIQQEK
                     MASIGQLAAGVAHEINNPIGFVSSNLRTLAKYADSVTGFFKELQRLLADAPAGSPLHA
                     LKDQWR"
     gene            complement(771..>1262)
                     /locus_tag="PK362_10890"
     CDS             complement(771..>1262)
                     /locus_tag="PK362_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC75373.1"
                     /translation="EYVNARRSTVSRVLETIVQRQDQFLRSGHQEDLRDLTQEELARR
                     LGVHSSTISRIVASKSVLTPWNEERRIKDFLGRRSVEGVLAKIQDLVEEEAGLIRSGV
                     LPGPFRDVELCRALAERQGPRIALRTMSKYRSLLQIPNVYDRARTFSRPAPPASPPPA
                     LKS"
BASE COUNT          229 a          355 c          426 g          252 t
ORIGIN      
        1 cccgccactg gtctttgagg gcgtgcaggg gggaaccggc gggggcgtcc gccagcaggc
       61 gctgcaattc cttgaaaaag cccgtcacgc tgtcggcgta tttggcgagg gtccgcaggt
      121 tggaagacac gaagccgatg gggttgttga tttcgtgggc cacgccggcc gccagttgac
      181 cgatggaggc cattttttcc tgttgaatga ttcgggattg ggtttccttc aacgtggaat
      241 tcatgcgctc cagttcccgg gccgtgtgga gcaaatgctc ctcggttttc cggcggctca
      301 aggcgtagcg gagcgatcgt tccaaaaggg aggcgtcgta ttcgcctttg atgagaaaat
      361 cgtacgcccc ttcgcgcatc acctgcaaat cgacctcccg gtcgcccttt tcggtcagca
      421 cgatgacggg cgcctccggg ccccggtcct tgagggactg gagaaaatcc aggccggttt
      481 tgccccccag atggtattcg acaagaacca gggcgtaggc ccccttggaa agggcccgca
      541 atccggcctc gtaatcgggg gcccaatcga ggcaataggt gtcggtttca aattgctcca
      601 gaagcgcccg ggcggcgttg aaatcttttt cgtcgtggtc gaccaacagg atcggaaccg
      661 gtgatgtgga catccttccc cctcgaagtc ggcggcatcc cagggacgcg cggacgaatg
      721 gacaacgacg gcgatggccg cgttgttagg ggaattatcg gtgggggctt ctaagactta
      781 agagccgggg ggggcgaggc cggaggagcc ggccgggaga aggttcgggc ccggtcgtat
      841 acgttgggaa tttgcagcag ggagcgatat ttggacatgg ttcgaagggc gattcgaggc
      901 ccttgccgtt cggccagggc gcggcagagt tccacgtccc gaaagggccc ggggaggacg
      961 ccggatcgga tcaacccggc ctcctcttcc accaggtcct ggattttcgc caggacgccc
     1021 tccaccgaac ggcgccccag aaaatctttg atgcgccgtt cctcgttcca gggcgtcagg
     1081 acggatttgg aggcgacgat gcggctgatg gtgctggaat gcacgcccaa tcggcgggcc
     1141 agttcctcct gggttaaatc ccgcaaatct tcctggtggc cggaccggag aaattggtcc
     1201 tggcgctgga cgatggtttc caacacccgg ctgacggtgg agcgccgggc gttgacgtat
     1261 tc
//
LOCUS       DAOQLS010000712         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_94763_length_1262_cov_2.914664, whole genome shotgun sequence.
ACCESSION   DAOQLS010000712 DAOQLS010000000
VERSION     DAOQLS010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94763_length_1262_cov_2.914664"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PK362_10895"
     CDS             complement(<1..504)
                     /locus_tag="PK362_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC75374.1"
                     /translation="MNLLEVSDLSKTYAVEAGLFRRRVGAVKALAGVSFSLESGRSLG
                     LVGESGSGKTTLARILAGFLDADGGSVSWEGRPRESYSRNEWASRVQMVFQDPSASLN
                     PKLRVRTQLDEALGQRARWDRTALSGRELKERRVRLLNDVGLPPDAGEAYPHQFSGGQ
                     KQRIAIAR"
     gene            complement(501..>1262)
                     /locus_tag="PK362_10900"
     CDS             complement(501..>1262)
                     /locus_tag="PK362_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC75375.1"
                     /translation="GRILFKGRDVLALADRELRSLRGGAVATVFQDPFSALNPVLTVG
                     EQIEEVLELHAGGRNPARVRDLLARVRLPEPDRIYSSYPHQLSGGQRQRVCLAMAVAG
                     GPELLIADEPTTALDVTVQREILDLLDSLRRDLGMALLLVTHNIGLLSERTDRLGIMY
                     AGELVEVGPTRRVLSDPRHPYTQGLLKSLPRLTKTADRLPALPGQPPDLGRLPAGCPF
                     RPRCPGAFERCEKESPVLRPVKGAADRAAACHLLP"
BASE COUNT          224 a          429 c          419 g          190 t
ORIGIN      
        1 ccgggcgatg gcgatgcgtt gcttttgccc gccggaaaat tgatggggat aggcctcgcc
       61 cgcgtccgga ggcaatccca cgtcgtttaa gagccggacc cggcgctcct tcaattcccg
      121 gccggaaaga gccgtccggt cccaccgggc ccgctggccc agggcttcgt ccaactgggt
      181 ccgcacccgc aacttcgggt tcagggaggc tgagggatcc tgaaacacca tttgcacgcg
      241 ggaggcccat tcgttccggg agtagctttc acggggccga ccttcccagg aaacgcttcc
      301 cccgtcggcg tccaggaaac ccgccaggat tcgggccaag gtggtcttcc ccgaaccgga
      361 ttcccccacc aaccccaggg accgcccgct ttccagggaa aaggagacgc ccgccagggc
      421 tttcaccgcg cccacccgtc ggcggaaaag ccccgcctcc acggcgtagg ttttggacag
      481 gtcgctgacc tcgagcaaat tcatggcagc aggtggcagg ccgcggcccg atcggcggcg
      541 cccttcacgg gccgaagaac cggggattcc ttttcgcatc gttcaaaggc ccccgggcac
      601 cgggggcgaa agggacaccc ggcgggaagg cggcccaggt cgggcggctg gcccggcagg
      661 gccggcaggc ggtcggcggt cttggtcaac cgcggcaggg attttagaag cccctgggtg
      721 taaggatggc gggggtccga caggaccctt cgggtcgggc ccacttccac caactccccg
      781 gcgtacatga tgcccagccg gtccgtccgc tccgaaagaa gcccgatgtt gtgggtcacg
      841 agcaggaggg ccatgcccaa atcccgccga agggaatcca gcaagtccag aatttcccgt
      901 tgcacggtga cgtccagggc cgtggtgggt tcgtcggcga tcagcagttc cggccccccc
      961 gccacggcca tggccaggca cacccgctgg cgctggccgc cggaaagttg atgggggtag
     1021 gaagaataaa ttcggtccgg ctccggcaat cgaacccggg ccagaaggtc ccggacccgg
     1081 gcggggttcc gccccccggc gtggagctcc aacacttcct cgatttgttc ccccacggtg
     1141 agcaccgggt tcaaagcgga aaaggggtct tgaaacaccg tggccacggc cccgccccga
     1201 agggaacgaa gttcccggtc ggccagggcc agcacgtccc ggcctttgaa gaggatgcgt
     1261 cc
//
LOCUS       DAOQLS010000713         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_95050_length_1260_cov_3.179253, whole genome shotgun sequence.
ACCESSION   DAOQLS010000713 DAOQLS010000000
VERSION     DAOQLS010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95050_length_1260_cov_3.179253"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..>1260
                     /locus_tag="PK362_10905"
     CDS             92..>1260
                     /locus_tag="PK362_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75376.1"
                     /translation="MLAAVGTGLAAYLLRDTAAGDVALALPSSFLWDNGLTNAVSLAV
                     SPFLFGALGGPAGDEKVRLLSPAREAYFDRLREVVNTGGTVLYAAGGVDLSGAFRIWA
                     PAKLILVSTTRLPRAEALRAAFADALPPAETVYSRQKRQHGYALGSDLTLPANALLAL
                     TAELNELGVDPKTVLVSVDFKGRIVLSFRWEGKPRVAYLVEAKVQETGAYADLLEGGL
                     DGYFHKAAMAIPLNYSAKEPFWSLVGRALAKKAPAPGKANLLTDDQGKAPAAPARDRA
                     GQFPLAARPVEVPGESALLAALYGVARTGEDVSWLSADDAYGTRLRARVLEGDGGLPA
                     PTLEPKEAAAEPARPLPSVVAEPSSDGERWILRHDGKYIGEAAVVLSADRAVVRSL"
BASE COUNT          184 a          410 c          437 g          229 t
ORIGIN      
        1 ctacggctgg cgcctccggg ttcgccggtt ttccgaaaat ccgccccggg cccgaccgat
       61 tccgagccgg ggcccttcca cggcgctgaa aatgttggcg gccgtcggga ccgggttggc
      121 ggcctatctt cttcgggaca ccgccgccgg cgatgtggcc ttggcccttc cctcgtcgtt
      181 tttgtgggac aacggcttga cgaacgccgt gagtttggcc gtttcgccgt ttttgttcgg
      241 cgcccttggg gggcccgcgg gggacgaaaa agtccggctc ctgtcgccgg cccgggaggc
      301 gtacttcgac cgccttcggg aggtggtgaa caccggcggc acggtccttt atgccgcggg
      361 cggggtggac ctctccggcg ccttccgaat ttgggcgccc gccaagttga tcctcgtatc
      421 aacgacgcgt ctcccccggg ccgaagccct gcgcgcggct ttcgccgacg ccttgccccc
      481 cgcggagacc gtttacagcc gccaaaaacg tcaacacgga tacgccctgg gttccgattt
      541 gaccctgccc gccaacgcgc tcctggccct gacggccgaa ttgaatgaat tgggggttga
      601 cccgaaaacc gtccttgttt ccgtcgattt taagggccga attgttttgt ccttccggtg
      661 ggaagggaaa ccccgggtgg cgtacttggt ggaggccaag gtccaggaaa ccggggccta
      721 cgccgacctt ctggagggtg gactggacgg ctatttccac aaggcggcca tggcgattcc
      781 gttgaattac tcggccaagg aacctttctg gagtttggtg ggccgcgccc tggcgaaaaa
      841 agcgccggcg ccgggaaaag ccaatctgtt gaccgacgac caaggcaaag cgcccgcggc
      901 cccggcccgg gaccgcgcgg gtcaatttcc gctggcggcc cgccccgttg aagttcccgg
      961 ggagtcggcg cttttggcgg ccctctacgg cgtcgcccga acgggcgagg acgtgtcctg
     1021 gctttcggcg gacgacgcct acggcacccg ccttcgggcc cgcgttctgg agggcgacgg
     1081 gggacttccg gcgccgacgc ttgaaccgaa agaggccgcg gcggagccgg cgcggccgtt
     1141 gccgtcggtg gtggccgaac cctcgagcga cggggaacgc tggatcctcc ggcacgatgg
     1201 aaagtacatc ggcgaggccg cggtggtgct ctcggcggac cgggcggtgg tgcgaagcct
//
LOCUS       DAOQLS010000714         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_95331_length_1258_cov_3.838736, whole genome shotgun sequence.
ACCESSION   DAOQLS010000714 DAOQLS010000000
VERSION     DAOQLS010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95331_length_1258_cov_3.838736"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..128)
                     /locus_tag="PK362_10910"
                     /pseudo
     CDS             complement(1..128)
                     /locus_tag="PK362_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
     gene            complement(170..535)
                     /gene="acpS"
                     /locus_tag="PK362_10915"
     CDS             complement(170..535)
                     /gene="acpS"
                     /locus_tag="PK362_10915"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNC75377.1"
                     /translation="MTPPSLGVDIVEVKRIARLIREPRFLARVFSDREVAYCRAKKNA
                     AQHFAVRFAAKEAVYKALGRAGVAHKDISVKNEPSGKPGVELAPRLRSLESRLTLTLS
                     HTAEHAVAVALFSPPARRR"
     gene            complement(545..>1258)
                     /locus_tag="PK362_10920"
     CDS             complement(545..>1258)
                     /locus_tag="PK362_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75378.1"
                     /translation="GAVIGVFAFVRSRRRRFAVGAGAGAAAVFLAGVLRPAAPDFRLV
                     WWGRDAVLVQTPAGSGLFDSSPDGALPAAAGFLRGQGVDRPVWRCSPVAHPLRFAASG
                     GVARGREGDVRWTAFVGGPRDGVLYLLERGSSRALLGFEVSEALAVAAAPRLPSVVDV
                     LGWRPRAGAPAALDRLSRTTPRAVVFQGRSPSASLRRTGTAMYFPGRGAVAWTGGRDG
                     GRLSEFAAASPPVRRPSVW"
BASE COUNT          229 a          454 c          446 g          129 t
ORIGIN      
        1 cgtggaacgg ccaaggtgac caggcccgcg cccccccgca gagccccgac ggccgccagg
       61 cgcggggcgc ccgacatgcc cggggcgccg ccgaccacga gcacgtggcc gaaatcgttt
      121 ttgtgggaat cccggggacg gggcggaagc caggaccggg gaacgacggt catcggcggc
      181 gggcgggcgg tgagaaaagg gcgacggcca ccgcgtgctc ggcggtgtgg gacagcgtca
      241 gcgtcagccg ggattccagg gaacgcagtc gcggggccag ttcgacgccg ggtttccccg
      301 agggctcgtt tttgaccgag atgtccttgt gcgcgacgcc cgcgcgcccc agggctttgt
      361 acaccgcttc cttcgcggcg aaacgcacgg caaaatgctg ggccgcgttt tttttggccc
      421 ggcaataggc cacctcgcgg tcggaaaaga cccgggcgag aaaccggggc tcgcggatca
      481 aacgggcgat gcgtttcact tccacgatgt cgacgccgag ggagggaggg gtcatgccgt
      541 tattttacca aaccgacggc cgtcgcaccg ggggcgacgc cgccgcgaat tccgacaaac
      601 gaccgccgtc ccgtccgccc gtccaggcca ccgccccccg gccgggaaaa tacatcgcgg
      661 tccccgtccg acgaaggctc gccgacggcg atcgcccttg gaatacgacg gcgcgggggg
      721 tcgtacggct caatcggtcg agggcggcgg gcgccccggc ccggggccgc caacccagga
      781 cgtccacgac ggacggaagg cgcggggccg cggcgacggc cagggcctcg gagacctcaa
      841 aacccagaag cgcccgggac gatccccgtt ccagcaaata caaaacgccg tcccggggcc
      901 ctccgacgaa agccgtccac cgaacgtcgc cctcccgacc ccgggccacg ccgcccgacg
      961 ccgcgaaccg aagagggtgc gcgaccgggg aacatcgcca gaccggacga tccacgccct
     1021 ggccccggag aaatccggcc gcggccggaa gcgcgccgtc cggcgaagaa tcaaagaggc
     1081 cggacccggc gggggtttgc acgaggacgg cgtcccgccc ccaccacacg aggcgaaaat
     1141 ccggcgcggc gggtcgaagg actcccgcca ggaaaaccgc ggccgccccg gcgccggctc
     1201 cgacggcgaa tcgacgtcgc cgggaccgga cgaaagcgaa gacgccgatc accgcgcc
//
LOCUS       DAOQLS010000715         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_95356_length_1258_cov_3.248545, whole genome shotgun sequence.
ACCESSION   DAOQLS010000715 DAOQLS010000000
VERSION     DAOQLS010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95356_length_1258_cov_3.248545"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>1258
                     /locus_tag="PK362_10925"
     CDS             36..>1258
                     /locus_tag="PK362_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNC75379.1"
                     /translation="MTGVACAFRDSVPIMILTAQIALAAFGKGAAQESSPLGIDIVDM
                     YKDMTKASVMLMAPEKIAELTRHLLRTGLSGRPGPIHLSLPADMVKRPVPVDLRTPSQ
                     YRPQAEVFDRRSVKEAAKLLLRAHRPAILAGYGVHLSRAYEELKVLAERLKIPVATTP
                     KAKGVFPEDHILSLGIFGLAGSPQADAVLLSDETDLVLAIGTSFSEAGSHAWDPRFMN
                     GKELMHIDVDPREIGKNFPVKVGLVGDAKQVLLELNYQIERDSRWLDTTMDFGYRLAA
                     VRALKAMHPRIMDAKSLEDESLPLRPQRVIAEMRRALPDNAIVFCDIGNVMAWALHYF
                     PVREPGTFFINMGFGSMGHAVAGVIGGKLAAKDRPVVALVGDAAFAMNGMEIHTAVEN
                     NIPVIWVVMNNGGHG"
BASE COUNT          239 a          404 c          378 g          237 t
ORIGIN      
        1 gttgcacgac caccggtccg ggcgccacga acgccatgac gggcgtcgcc tgcgcctttc
       61 gggattcggt ccccatcatg attttaaccg ctcaaatcgc cttggccgcc ttcggcaagg
      121 gcgccgccca ggaaagttcg cccctgggca tcgacatcgt ggacatgtac aaagacatga
      181 ccaaggccag cgtgatgctg atggccccgg agaagatcgc cgaattgacc cgccacctgc
      241 ttcgaacggg cctgtccgga cgcccagggc ccattcattt aagcctcccg gccgacatgg
      301 tgaagcggcc ggtccccgtc gatcttcgaa cgccgtccca ataccgtccc caggcggagg
      361 tgttcgaccg gcgttcggtg aaggaagccg cgaaactcct gcttcgcgcc catcgccccg
      421 cgattttggc gggctacggg gtgcatctct cccgggccta cgaggagctc aaggtgttgg
      481 ccgagcgcct caaaatcccc gtcgccacca cgcccaaagc gaaaggggtg ttccccgagg
      541 accacatctt gtccctgggc attttcggtc tggccggttc gccccaggcc gacgcggttc
      601 tcctttccga tgagacggac ttggtgctgg ccatcggcac cagtttcagc gaagccggtt
      661 cccacgcctg ggacccccgc ttcatgaacg gcaaggaatt gatgcacatc gatgtggacc
      721 cccgggaaat cgggaaaaat ttccccgtga aggtgggtct ggtgggcgac gccaagcaag
      781 tcctcttgga attgaactat caaatcgagc gcgattcccg ttggctggac accaccatgg
      841 atttcggata ccgactggcg gcggtgcggg ccctaaaagc catgcacccg cgcatcatgg
      901 acgccaaaag cctggaggac gaatccctgc ccctccgccc ccagcgggtg atcgccgaaa
      961 tgcggcgggc cctccccgac aacgccatcg ttttttgcga tatcggaaac gtcatggcct
     1021 gggctctgca ctatttcccg gtccgagaac ccggcacgtt ctttatcaac atgggtttcg
     1081 gctccatggg acacgccgtg gccggtgtca tcggcgggaa attggccgcc aaggaccgtc
     1141 cggtggtggc gttggtgggc gacgccgcct tcgccatgaa cgggatggaa attcacacgg
     1201 cggtggagaa taatatcccc gttatttggg tcgtgatgaa caacggcgga catggcat
//
LOCUS       DAOQLS010000716         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_95462_length_1257_cov_4.180532, whole genome shotgun sequence.
ACCESSION   DAOQLS010000716 DAOQLS010000000
VERSION     DAOQLS010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95462_length_1257_cov_4.180532"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PK362_10930"
     CDS             complement(<1..219)
                     /locus_tag="PK362_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC75380.1"
                     /translation="MARIIAVANQKGGVGKTTTAVNLAASLAHLGQETLLIDMDPQAN
                     LTSGLGIARDGLEKHIYHVLLDNAGLEEV"
     gene            complement(225..518)
                     /locus_tag="PK362_10935"
     CDS             complement(225..518)
                     /locus_tag="PK362_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75381.1"
                     /translation="MPAFDVTCPCCRATLTVSGDAQSVLKFKEAPKPKAVSDLTEAAR
                     ALQKDPERREELFKQSLETEKNKGDKLKKSFNDLFKRAAEEPLERPTRDMDLD"
     gene            complement(553..>1257)
                     /locus_tag="PK362_10940"
     CDS             complement(553..>1257)
                     /locus_tag="PK362_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNC75382.1"
                     /translation="ASLALHGGAFLFLNHRAGGGDGAPLWATADAIEVDLTRPFRLTA
                     DPRLARLSANPGTGAPLVDKPTPGPTLPGGGVAAKGVEWTAPGPKTTALTTPEIEGNV
                     LSSSTGPSTGTGLVEGPSQGLGGLGTGGEGEVDWVYLTELPKLLNREEMARNIRRFYP
                     EEERRAGREGQVVARIHLNREGRVSSLDVERSAGAAFDAAAQKVLALAQFSPAKAGDR
                     TVAVKIRQAVAFRLED"
BASE COUNT          190 a          404 c          421 g          242 t
ORIGIN      
        1 gacctcctcc aggcccgcgt tgtccaggag cacgtggtag atgtgtttct ccaggccgtc
       61 ccgggcgatg ccgaggcccg acgtgaggtt ggcctggggg tccatgtcga tcaaaagcgt
      121 ttcctggccc aggtgcgcca gggaggcggc caggttcacg gcggtggtcg ttttccccac
      181 cccgcccttc tgattggcga cggcgatgat ccgggccatg ggcgctagtc cagatccatg
      241 tcgcgggtgg gccgttccag gggctcctcg gccgcgcgct taaagagatc gttgaaggat
      301 tttttcaatt tgtcgccttt gtttttttcg gtttccagcg attgcttgaa cagttcttcc
      361 cggcgctccg ggtctttttg aagggcccgg gccgcttcgg tcaagtccga caccgctttg
      421 ggtttcgggg cctctttgaa tttaaggacg ctctgggcgt cgccgctcac ggtcaaggtc
      481 gcgcggcaac aggggcaggt gacgtcaaag gccggcatgg gcggaattat accaaagtcc
      541 gacggaaatc gatcagtctt ccaggcgaaa agccacggcc tggcggattt tgaccgccac
      601 cgtccggtcc cccgctttgg ccggggaaaa ttgggccagg gccaggactt tctgggccgc
      661 ggcgtcaaag gccgccccgg ccgaccgctc cacgtccagg gagctcaccc gaccttcccg
      721 gtttaaatga atccgcgcca ccacctggcc ctcgcgaccg gcccggcgct cctcctcggg
      781 gtagaagcgc cggatgtttc gggccatttc ttcccgattg agaagttttg gaagttcggt
      841 caagtagacc cagtccactt ccccctcgcc gcccgtgccc agcccgccca agccctggga
      901 aggcccctcc accagcccgg ttcccgtcga cggtcccgtg gaggaggaca aaacatttcc
      961 ctcgatttcc ggggtcgtta aagcggtggt tttggggccg ggggcggtcc attcgacgcc
     1021 cttggccgcc acgccgcccc ccggcaaggt cggtccgggg gtgggtttgt cgaccaaggg
     1081 agcgccggtt ccggggttgg cggacaggcg ggccagccgg ggatcggccg tcaggcggaa
     1141 ggggcgggtg agatcgactt cgatggcgtc ggccgtggcc cagaggggcg cgccgtcgcc
     1201 gcccccggcc cggtggttca aaaatagaaa cgcccccccg tgcagggcca gggaagc
//
LOCUS       DAOQLS010000717         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_96749_length_1249_cov_2.758794, whole genome shotgun sequence.
ACCESSION   DAOQLS010000717 DAOQLS010000000
VERSION     DAOQLS010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96749_length_1249_cov_2.758794"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PK362_10945"
     CDS             complement(<1..474)
                     /locus_tag="PK362_10945"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNC75383.1"
                     /translation="MIHSFVRLIEALQGNPLSVPVFILLYAGGCFIAPVSLFPVAGGV
                     LFGFGPGLAINLAAALLGASGAFGLARVLGRHRLEPWLRRSGQSHPWAETLENPAPGA
                     FILLRLVGFPPFVITNYLAGLSFMPFRVFLWTSAIGLLPWTFVMTFFSGTFWAILL"
     gene            complement(471..>1249)
                     /locus_tag="PK362_10950"
     CDS             complement(471..>1249)
                     /locus_tag="PK362_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75384.1"
                     /translation="NPNIFWIAVLNGFFGSLAAFGTDQDLMQRLLTLETRKDSQKTMV
                     GTIGVSLGVLTIYLTIGTGLYVYFRQPGSVSFPANPDAVFPHFIGHVMPSVLKGWLLS
                     AIVLAGIDSPLASLATSFVTDLYRPYVEARGRRPTDREILRWSRWGVAVFGLVLAALA
                     YGFSFYKNILWLAFKIGGVTFGSLLGVFLLGLLTRRRSDRWNAVAMVSSALLNLVLLV
                     LSEKKILPMGWSWLVVLGTATTFGLASLLSRPGAREGAAA"
BASE COUNT          335 a          388 c          362 g          164 t
ORIGIN      
        1 cagcaaaatc gcccaaaagg ttccggaaaa aaacgtcata acgaaggtcc acggcaacaa
       61 cccgatcgcg ctcgtccaca aaaagacgcg aaaaggcatg aaggaaagtc cggccaaata
      121 attcgtaatc acgaagggcg ggaatcccac cagtcggagg aggataaaag ccccgggcgc
      181 cggattttcc agggtttccg cccacggatg ggattgtccc gaacgacgca accagggctc
      241 caatcgatga cgcccgagaa cccgcgccag accgaaagcc ccgctggccc cgagaagcgc
      301 ggccgccaga ttaatcgcaa gcccgggccc gaacccgaac aggacgccgc ccgccaccgg
      361 aaagaggctc accggagcga tgaagcaccc cccggcgtag agaagaatga acacggggac
      421 cgaaaggggg ttcccttgca gcgcctcaat gagccggacg aaactgtgaa tcacgcggcg
      481 gccccctccc gcgcgcccgg ccgggacagg agggacgcga gcccgaaagt cgtcgccgtt
      541 cccagaacca ccagccaact ccaccccatg ggcagaattt ttttctccga aagaacgagc
      601 aggacgaggt tcaagagggc gctcgaaacc atggccacgg cgttccatcg atcggaccgg
      661 cgccgcgtca ggagccccaa caggaaaacc cccagcaacg acccgaaggt caccccgccg
      721 attttaaaag ccagccacaa gatgtttttg taaaaactga acccgtaggc gagagcggcc
      781 agcaccaggc caaacacggc gaccccccat cgggaccatc gaaggatttc gcggtccgtc
      841 ggacggcgac cgcgggcttc cacataaggc cggtagagat ccgtgacaaa ggacgtggcc
      901 agggaggcca gcggcgaatc gatgccggcc aaaacgatgg cggacaacag ccaccctttg
      961 aggaccgacg gcatgacgtg tccgatgaaa tgcggaaaaa cggcgtccgg attggccggg
     1021 aacgaaacgg atcccggttg gcgaaagtaa acataaagac cggtgccgat cgtcaaatag
     1081 atcgtcagaa cgcccaggct caccccgatg gtccccacca tggttttttg gctgtctttg
     1141 cgcgtttcca aggtcaaaag cctttgcatc aaatcctggt cggtgccaaa agccgccagc
     1201 gatccgaaaa aaccgtttaa aacggcgatc cagaagatgt tggggttgg
//
LOCUS       DAOQLS010000718         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_96835_length_1248_cov_4.402347, whole genome shotgun sequence.
ACCESSION   DAOQLS010000718 DAOQLS010000000
VERSION     DAOQLS010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96835_length_1248_cov_4.402347"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PK362_10955"
     CDS             <1..682
                     /locus_tag="PK362_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75385.1"
                     /translation="DNPPIPGSPARRVPFAAPVTDPFADAVMPDVAFDVGAQIYAVVW
                     NQGDYRSDFESPGQPGEIFAQSISRTGVRNLPENLRISAVGFDGVETAYLPNVAARAG
                     TGEFLVVFGGRGQYPLDLNENETYGQRLSAPVVNGDLPPLPPPPVVGPNPFRPNQSPV
                     SVKNAGANGRVQVYDFAGEKIRELIADGAGDVQWDGRNDAGEIVATGTYFLHFDVGDR
                     IVKVGIQN"
     gene            679..>1248
                     /locus_tag="PK362_10960"
     CDS             679..>1248
                     /locus_tag="PK362_10960"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC75386.1"
                     /translation="MKNLARAAGIALFFAPALSAASFNGNARGTTAGVLLRMPPSPRS
                     AALGEVRASLSGDASAVLTNPAALTDVRRLSVSLAHSPYLEDTGFGHVTFAQKMGFLA
                     IGGGVQYLSAGSLTRSDSGTNALGNFKPGDAAAVAGGALKIGALSLGGAFKWVRSEVD
                     QSATAMAVDLGLTASGGTDRKWRLGATATN"
BASE COUNT          192 a          439 c          400 g          217 t
ORIGIN      
        1 ggacaacccc ccgatccccg gatcgcccgc ccggcgggtt cccttcgccg cgccggtgac
       61 ggatcccttc gccgacgcgg tcatgcccga cgtggccttc gatgtgggcg cgcaaatcta
      121 cgcggtcgtt tggaaccagg gcgattaccg ttccgatttc gaatctccgg gacagccggg
      181 cgaaattttc gctcaatcca tctcccggac cggcgtccgt aatttgcccg aaaatcttcg
      241 gatcagcgcc gttggatttg acggcgtgga gacggcgtac ctgcccaacg tggcggcccg
      301 ggccggaacc ggggagttcc tggtggtctt cggcggtcgc gggcaatatc ccctggattt
      361 gaatgaaaac gaaacctacg gccagcgact ttccgccccc gtggtgaacg gcgacctgcc
      421 gcccctgccc ccgccgccgg tggtggggcc caatcccttc cggcccaatc agagcccggt
      481 gtccgttaaa aacgcgggtg ccaacggccg cgtgcaggtc tacgacttcg ccggggaaaa
      541 gatccgggaa ttgatcgccg acggcgccgg ggacgtccag tgggacggcc gcaacgacgc
      601 cggggaaatc gtcgccacgg gcacctactt cctccatttt gacgtgggcg accgcatcgt
      661 gaaagtgggg attcaaaatt gaagaacctt gcccgcgccg ccgggatcgc cctctttttt
      721 gcgccggccc tgtcggccgc gtcctttaac ggcaacgccc ggggcaccac ggcgggcgtc
      781 cttctgcgca tgccgcccag cccgcgctcg gcggccctgg gggaggttcg cgcgtccctt
      841 tccggcgacg cctcggcggt gctcaccaac cccgcggccc tgaccgacgt ccggcggctc
      901 tcggtgtccc tggcccattc cccttatttg gaagacaccg gcttcggcca cgtcactttc
      961 gcccaaaaaa tgggttttct tgccatcggc ggcggcgtcc aatatttgtc cgccgggtcc
     1021 ctcacccgca gcgattccgg caccaacgcc ctgggcaact tcaaacccgg cgacgcggcg
     1081 gcggtggcgg ggggggccct caaaatcggc gcgctttccc tgggcggggc cttcaaatgg
     1141 gtccgctccg aagtggacca gagcgccacg gccatggccg tggatttggg cctgaccgcc
     1201 tcgggcggga cggaccggaa gtggcgcctg ggggccaccg ccaccaat
//
LOCUS       DAOQLS010000719         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_97004_length_1247_cov_4.079698, whole genome shotgun sequence.
ACCESSION   DAOQLS010000719 DAOQLS010000000
VERSION     DAOQLS010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97004_length_1247_cov_4.079698"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK362_10965"
     CDS             complement(<1..298)
                     /locus_tag="PK362_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNC75387.1"
                     /translation="MVGPEELDRLRLAADGRVESVGPRFAERLGYSPGELAGQPLKLI
                     LGRWCPDEPTAEALRALFEGPSPKPFLNLTGAEVPLRVHVESAAGGAVALRAEPP"
     gene            complement(320..682)
                     /locus_tag="PK362_10970"
     CDS             complement(320..682)
                     /locus_tag="PK362_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75388.1"
                     /translation="MRALVVDDETDVRKLIGLMLTRLGHQVREVDTGEKALEVLRGDP
                     RPDVVLLDWSMPGMDGLTTLKAIRTEDALSTLRVMMVTGMNDMDDVSAALDAGADEYL
                     MKPISARALKEKLAILGL"
     gene            complement(728..1090)
                     /locus_tag="PK362_10975"
     CDS             complement(728..1090)
                     /locus_tag="PK362_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75389.1"
                     /translation="MKAFVIDDSRAVRTILRRLLTELGFEAREAENGKDGLAALREGY
                     RPDVALVDWNMPEMDGVSFIRAVRAEPAYAAVRLVVITTEMEVGRVAEALDAGAQEYV
                     LKPLTKETLRDKLAALGL"
BASE COUNT          209 a          455 c          371 g          212 t
ORIGIN      
        1 gcggaggctc cgcccgcagg gcgacggccc cgccggcggc cgattcgacg tggacccgaa
       61 gcggaacttc ggcccccgtg aggttcaaaa agggtttggg cgacggacct tcaaagaggg
      121 cgcggagggc ctccgccgtc ggttcatccg ggcaccatcg accgagaatc aatttcaggg
      181 gctgccccgc caattcgccc ggggagtatc ccagccgctc ggcgaagcgg gggccgacgc
      241 tttcaacgcg tccgtcggcg gccagccgca gccggtcgag ttcttcgggt ccgaccatgc
      301 ccatcgggtt cctttcgtcc tacaggccga gaatcgccaa tttttccttg agcgcccggg
      361 cggagatcgg cttcatcaaa tattcgtccg cgcccgcgtc cagggccgcc gacacgtcgt
      421 ccatgtcgtt catgcccgtc accatcatga cccgaagggt cgacaaggcg tcttcggtcc
      481 gaatggcttt caacgtggtc aacccgtcca tgccgggcat gctccaatcc aggaggacga
      541 cgtccgggcg cgggtcgccg cgcaggactt ccaaggcctt ttcgccggtg tccacttccc
      601 gcacctggtg acccaagcgg gtcagcatca atccgatcaa cttccgaacg tccgtttcat
      661 cgtccaccac caaagcgcgc accgaaggac ctccttgctc gtcgatcaat tcgtccgggc
      721 ccgggatcta caaccccagg gccgccaatt tatcccgcaa cgtttccttg gtgaggggct
      781 tcaggacgta ttcctgcgcc cccgcgtcca aggcctctgc cacccgcccc acttccatct
      841 ccgttgtaat aacgaccaac cgcaccgcgg cgtaggccgg ttcggcccgc acggcgcgaa
      901 tgaaggacac gccgtccatt tcgggcatgt tccaatccac cagggcgacg tcgggtcggt
      961 aaccctcgcg gagcgccgcc aggccgtcct tgccgttttc cgcctcccgc gcttcgaacc
     1021 ccagttcggt caacaggcgc cggagaatcg ttcgcaccgc ccgggaatcg tcgatcacga
     1081 aggccttcac ggcctggccc ccttggccca acgttcgagg tccgcttgaa ccggcgacgg
     1141 cgcgtcgggt tcccgacccg cggcgccgat gaccgcgaga aaccgttgaa tgtccgtggc
     1201 cgcccgcccc aacagggcga gcagcgccgg atcgggaacg ccgccgc
//
LOCUS       DAOQLS010000720         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_97047_length_1247_cov_2.893456, whole genome shotgun sequence.
ACCESSION   DAOQLS010000720 DAOQLS010000000
VERSION     DAOQLS010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97047_length_1247_cov_2.893456"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK362_10980"
     CDS             <1..488
                     /locus_tag="PK362_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75390.1"
                     /translation="IGLMGIFWILLAGMVALFVFLKINNQYFLEEQFFELLAYAGFAL
                     VNVSIIAVVENKFQKTAVPFRDIALANACYLLLAILLAIIVWLVLEWLRRPGIPTAIL
                     LNLIALGIFVSLKQFLFNRIGLAMSNNLAYAIGYAFVLFAPIMALNLYVLLLGRGMNG
                     H"
     gene            521..>1247
                     /locus_tag="PK362_10985"
     CDS             521..>1247
                     /locus_tag="PK362_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNC75391.1"
                     /translation="MNKGIAIAGAGALLMVAGALSAWGRSFFRRETPEKYFTGPHLEI
                     ARAIARRDLDKVKSLCQGVEIDKPFKKNMTLMWFAMIKKNYEAVTLLTRLGSDLDGQV
                     AEGLGSPLDIAFNTPTTEYLRAMLDGGLAPNHTHPSHNGMMHRAVMDGTLDHLKLLLE
                     RGADVDQPDSLHRTALSNALAIVQADVALFLIERGADVNATMTNGVTIAWSLQKRIER
                     QEPGSPTAKKLELVKQAMEQRGVK"
BASE COUNT          257 a          345 c          361 g          284 t
ORIGIN      
        1 agatcggttt aatgggcatt ttctggatcc tcctcgcggg gatggtcgcc ctcttcgttt
       61 ttctgaaaat caataaccaa tattttctgg aagagcaatt ttttgaattg ttggcctacg
      121 cgggttttgc gctggtcaat gtttcaatca tcgccgttgt cgaaaacaaa ttccaaaaaa
      181 cagcggtccc ctttcgggac atcgccctgg ccaacgcctg ctacctcctg ctggcgatcc
      241 tactggccat cattgtttgg ttggtcttgg agtggctccg gcggccggga attccaacgg
      301 cgattctttt gaatctcatc gccctgggaa tattcgtctc tttaaagcaa tttcttttca
      361 atcggatcgg gttggcgatg tcgaacaatt tggcatacgc catcgggtat gcctttgtgt
      421 tgtttgcgcc catcatggcc cttaacctct acgtcctcct gttgggccgc ggcatgaacg
      481 gccattaagc ggatgaaaac ttttgtcggg ggggaaacgg gtgaataaag gaatcgccat
      541 cgccggcgcc ggggcgttgt tgatggtcgc cggcgccctt tccgcctggg ggcgctcctt
      601 ttttcgaagg gaaacgccgg agaagtattt caccggtccc cacctggaaa ttgcccgggc
      661 catcgctcgc cgggacctgg acaaggtcaa aagcctttgc caaggggtcg aaatcgacaa
      721 accctttaag aagaatatga ccctgatgtg gttcgcgatg ataaaaaaga attacgaggc
      781 ggtcacgctt ttgacgcggt tgggttccga cctggatggg caagtggcgg aggggctcgg
      841 cagccccttg gacatcgcct tcaatacgcc caccacggaa tatttgcgag ccatgttgga
      901 cgggggcctt gccccgaacc acacgcaccc ctcccacaac ggcatgatgc accgggcggt
      961 gatggacggc accctggacc acctcaagct ccttctggaa cggggcgcgg acgtggacca
     1021 gccggattcc cttcaccgga cggccctttc gaacgcgctg gccatcgttc aggcggacgt
     1081 ggcccttttc ctgatcgagc ggggcgcgga cgtcaacgcc accatgacca acggggtcac
     1141 catcgcgtgg tcgttgcaaa agcgaatcga gcggcaggag ccgggatcgc cgacggccaa
     1201 aaagttggag ctggttaaac aggccatgga acaacggggg gtcaaat
//
LOCUS       DAOQLS010000721         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_97173_length_1246_cov_4.769102, whole genome shotgun sequence.
ACCESSION   DAOQLS010000721 DAOQLS010000000
VERSION     DAOQLS010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97173_length_1246_cov_4.769102"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK362_10990"
     CDS             <1..485
                     /locus_tag="PK362_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNC75392.1"
                     /translation="RDPSSNAGAARRVLGPRGPVPGRRQYPFFLKPGDPRRDAGRFES
                     RLRRWFRNRRPDVVLLGMGEDGHTASLFPGFPALSEKRRWVAFVENAPKPPPGRLTLT
                     LPFFNRARHVLFLVQGPGKQRALRRVWRARGNTLPAGRVRPARGSLEWYVTREALRKG
                     "
     gene            600..>1246
                     /gene="tpiA"
                     /locus_tag="PK362_10995"
     CDS             600..>1246
                     /gene="tpiA"
                     /locus_tag="PK362_10995"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNC75393.1"
                     /translation="MPRRALMAGNWKMHKTIAESVDLVNALKTQAGSWPDRDVLVCPP
                     FTALAAAAQAARGTAIQVGAQNMNDNLQGAFTGEISATMIKDTGATFVVLGHSERRQF
                     YGETDALVNKKTKTALEQGLTPIVCVGETLAEREGGKTFSVIETQINGGLKGLSADQA
                     LRLVIAYEPVWAIGTGKTATPAQAQEVHAFIRRRLDALFGPKTADALRILYGGSKG"
BASE COUNT          224 a          401 c          392 g          229 t
ORIGIN      
        1 atcgggaccc ttcgagcaac gcgggcgccg cccggcgggt tctgggcccg cggggcccgg
       61 ttcccgggcg ccggcaatat cctttttttt tgaaaccggg cgatccccgt cgcgacgccg
      121 gccgttttga aagccgtctt cggcgatggt ttcggaaccg ccgtccggac gttgttttgt
      181 tgggaatggg cgaggacggc cacacggcct cgctcttccc cggatttccc gccctgtctg
      241 aaaaacgtcg atgggtcgcg ttcgtggaaa acgcccccaa accgcctccc ggccgtctga
      301 cgctgaccct tccgtttttc aatcgggccc gccacgtgct attcctggtg caggggccgg
      361 gaaaacaacg ggcccttcgt cgagtctgga gggcccgggg aaatacgctc ccggccggac
      421 gcgttcgtcc cgctcgcggg tccctggaat ggtacgtcac ccgggaagcc ctgcggaagg
      481 gatgattccc gcccggggaa ccgggtcgcc ggtccccatt gctccgacgg aaaggatgtt
      541 ctagaatacc cgacagaaac gcccttcttt tcactttgcc catcctcaaa ggagtctcta
      601 tgccccgtcg cgccttgatg gccggaaact ggaaaatgca caaaaccatc gccgaatccg
      661 tggatttggt gaacgctttg aaaacccaag ccgggtcctg gccggaccgg gatgtcctgg
      721 tttgcccgcc tttcacggcg ttggcggccg ccgcccaggc ggcgcggggg acggccatcc
      781 aggtcggcgc ccagaacatg aacgacaatc tccagggcgc cttcacgggg gaaatttccg
      841 cgacgatgat caaagacacc ggcgcgactt tcgtcgttct cggccactcc gagcgacgtc
      901 agttctacgg ggaaaccgac gcgttggtga ataagaaaac caaaacggcc ttggagcagg
      961 gtttgacccc cattgtctgc gtcggggaaa cgctcgccga acgcgaaggg gggaagacct
     1021 tttccgtgat cgaaacccag atcaacgggg gactgaaggg cctttccgcg gatcaggcgc
     1081 tccggctcgt gatcgcgtac gaaccggtgt gggccatcgg cacgggcaaa accgccacgc
     1141 cggcccaggc gcaggaggtt cacgctttta tccgccggcg gttggacgcc ttgttcgggc
     1201 ccaaaaccgc cgacgccctt cgaatcctct acgggggttc gaaggg
//
LOCUS       DAOQLS010000722         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_97790_length_1242_cov_4.093513, whole genome shotgun sequence.
ACCESSION   DAOQLS010000722 DAOQLS010000000
VERSION     DAOQLS010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97790_length_1242_cov_4.093513"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..791)
                     /locus_tag="PK362_11000"
     CDS             complement(15..791)
                     /locus_tag="PK362_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75394.1"
                     /translation="MATKTRVRSDLLTYVDREPTALWTLASLAAAAALVAARFHFASW
                     AVLGMALIATLFTFGVLWAITLYPRTYLGSRWASTAFMVALFLLCDSIGQVGFQIQWR
                     RSFEVNGAWKDFTDRGNAWRVRIPAKWQPFELYTPATGTVIFKPTRLTPAIEFSVTRH
                     PRTKDQDLSAAVEAYYLNLPKSTDTKILAGAPFDYAPGLDAYRMVHQDPQQALVLRQE
                     NVFLFHGDDLYMLTVTAVPAWFNRFPADLDRFIRSLRFTA"
     gene            942..1175
                     /locus_tag="PK362_11005"
     CDS             942..1175
                     /locus_tag="PK362_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75395.1"
                     /translation="MANALFNRLAEKVRQAVAEIHDLRKEKERLEAELALMTEENRRA
                     RRLLREHGGLIEERQKLRARVEKALLKLDKLRV"
BASE COUNT          295 a          333 c          402 g          212 t
ORIGIN      
        1 caaaaaaccg ggagtcaagc ggtaaagcgc aggctgcgaa taaaccgatc caggtcggcg
       61 gggaagcggt taaaccaagc cggaacggcg gtgaccgtca gcatgtaaag gtcgtcgccg
      121 tgaaaaagaa aaacgttttc ctggcggagg accaacgcct gttggggatc ttgatggacc
      181 attcgatagg cgtccagccc gggagcgtaa tcaaagggcg cgccggcgag gattttggtg
      241 tcggtgctct tgggcaaatt caggtagtag gcctccacgg ccgccgacag gtcctggtcc
      301 ttggttcggg gatggcgcgt tacggaaaat tcgatggccg gggtcagacg cgtgggtttg
      361 aagatcacgg tgcccgtcgc cggcgtgtac agttcgaacg gttgccactt cgcggggata
      421 cgaacccgcc acgcgttgcc ccggtccgta aaatccttcc aagcgccgtt cacttcaaaa
      481 gaccgccgcc actgaatctg gaagcccacc tgcccgatgc tgtcgcacaa taaaaagagg
      541 gccaccataa aggccgtgga agcccaccgg gaccccaaat aggtccgggg ataaagcgtg
      601 atggcccaga gaacgccgaa ggtgaagagg gtcgcgatca gcgccatccc caaaacggcc
      661 caggaggcaa agtgaaatcg cgccgcgacc aaggcggccg ccgcggccag ggaggccaac
      721 gtccaaaggg cggtgggttc ccggtcaacg taagtcagca aatccgatcg cacacgtgtt
      781 ttggtggcca tgcgtttatt atagacattc tggggggccg caacaatccg gccgggtccc
      841 gcggccgggc ggcgacggcc gcggcgatat ttaacaaaga acgttaactt accgacgatg
      901 agaagcttgt gggaggggcg gctctatgct aaattggccc cgtggcgaac gcgcttttca
      961 atcggttggc cgaaaaagtc cgccaggccg tggcggagat tcacgatttg cggaaggaaa
     1021 aggagcggtt ggaggccgaa ctggccctca tgacggagga aaatcgtcgg gcgcggcggt
     1081 tgttgcggga acacgggggg ttgatcgaag agcgccaaaa acttcgcgcc cgcgtcgaga
     1141 aagccctttt gaaattggat aaattgcgag tttgaccatg aaccccctcg ttcgagacaa
     1201 aatccgcgtc aatattttcg ggaaggacta cgaactcgac cc
//
LOCUS       DAOQLS010000723         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_97793_length_1242_cov_3.903960, whole genome shotgun sequence.
ACCESSION   DAOQLS010000723 DAOQLS010000000
VERSION     DAOQLS010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97793_length_1242_cov_3.903960"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..>1242)
                     /locus_tag="PK362_11010"
     CDS             complement(217..>1242)
                     /locus_tag="PK362_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNC75396.1"
                     /translation="EVVITGSSNWSEAALTRNHEANVLIRSKDVAREALAALRAIPRQ
                     APMADMATVEIPGEFVQDPKYLGRMANRGDERAFDVYLYLLKQGTATATVDYDDLAGS
                     LGLAMDPNAGRRQINKVLKKLVGYGLIRATTSYGKDAEVALNPLGDRRPARVPVAYWK
                     MGWDGRLGLAAKWFMVVGAFEGDVSTLRPRWSAAEKTLARRYHVSAWFISRGVMELRR
                     WNLLEVGYGTLPEGGPRLPNLYTPNEFYDPGLAEKKLEDLKRRYGEEKLARARRAAGA
                     VYEDWDLAGVEELIKLEEQFGVGRVEAGVRVVEGKRGDNPDRNLGYLIGTIRNLKFGP
                     LDVAPGR"
BASE COUNT          204 a          498 c          285 g          255 t
ORIGIN      
        1 gcctccaccc gctccccgtt gctcgtcccc atgaccgcct cctccgccgt cacgctccca
       61 tttgcgacgc caataattat taaagccacg cccgcccacc ggtttgcctt tttcatttat
      121 cttcctcgcc cacatcgaaa accgttttga tctttccttc cccccttgag ggggaaggcc
      181 cggatggggg gtgccgtcga atctgtttcc ctaggcctac ctacccggcg ccacgtccaa
      241 cggcccgaat ttcaaatttc gaatcgtccc aatcaaatac cccaaatttc gatccgggtt
      301 gtccccccgc ttcccttcga caaccctcac ccccgcctcc acccgaccca cgccaaactg
      361 ttcctccagc ttaatcaact cctccacccc ggccaaatcc cagtcctcat acaccgcccc
      421 cgcggcccgc cgggcgcgcg ccagcttctc ctcgccgtat cggcgcttca ggtcctccag
      481 ttttttctcc gccaacccgg ggtcgtaaaa ctcgttgggc gtgtaaaggt tcggcaatcg
      541 gggcccgccc tccggcaagg ttccatagcc cacttccagc aggttccacc gccgtagctc
      601 catcaccccc cggctgataa accaggccga cacatggtac cgcctcgcca gggtcttctc
      661 cgccgccgac caccggggcc gcagggtcga cacgtcgcct tcaaaagccc ccaccaccat
      721 aaaccacttg gccgccaacc ccaaccgccc gtcccacccc attttccagt aggccaccgg
      781 cacccgcgcc ggccgccggt cgccgagcgg gttcagcgcc acctccgcgt ctttcccgta
      841 ggaggtcgtg gcgcgtatca acccataccc taccagcttt tttaaaactt tgttgatctg
      901 tcttcgcccg gcgttcgggt ccatggccag gcccaaactc cccgccaaat cgtcgtagtc
      961 caccgttgcc gtcgccgtgc cctgtttcag caggtacagg taaacgtcaa aggcccgctc
     1021 gtctccccgg ttggccatcc gccccagata tttcgggtcc tgcacaaact cccccggtat
     1081 ttccaccgtg gccatgtcgg ccattggcgc ctggcgggga atggccctca gggccgccag
     1141 ggcctcccgc gccacgtctt tggatcggat caacacgttg gcctcgtggt tccgggtcag
     1201 tgccgcctcg ctccagttgc tggagcccgt gatcaccact tc
//
LOCUS       DAOQLS010000724         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_98047_length_1241_cov_2.320405, whole genome shotgun sequence.
ACCESSION   DAOQLS010000724 DAOQLS010000000
VERSION     DAOQLS010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98047_length_1241_cov_2.320405"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..820)
                     /locus_tag="PK362_11015"
     CDS             complement(71..820)
                     /locus_tag="PK362_11015"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNC75397.1"
                     /translation="MRRRAAGVLAVLALAVGPRAAEVDAPGDRLVFAQIRHAGDWDPY
                     PEAWSSAAALLRNATSLDPWPARRVVSLDGDALPESPFLVLAGRGDPELTDGEVARLR
                     EHLRAGGFLWIDNTEGGASNPFDRAAQALPSRLFPSETWRPVPADHALFRSFFLLPRA
                     GGRRQTDGRLWGLWAGDRLVAVYDRNDVLGALARDRVGQPLYPCEPGGEGQRREAQKT
                     FTNIVLFALTGTYKTDAVHQPFLERRFRERP"
     gene            complement(817..>1241)
                     /locus_tag="PK362_11020"
     CDS             complement(817..>1241)
                     /locus_tag="PK362_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75398.1"
                     /translation="EAASAPAPETVGGVWTGRRSGAVAALLRRPDGRSERLTGRAGEF
                     RAGPLELPGFYRLQFPSGGETVFAVNASRDAEEGNPARVPRDVPERWFGPGRTVWIGA
                     GDDLSRTLAGSPLRPLLRRGLLVLFVLETLMLFFRRVR"
BASE COUNT          211 a          439 c          442 g          149 t
ORIGIN      
        1 gacgacgccg ccttcgcgcg cctgctggcc gatccggcgg ccgtctccgc cgcgagggtc
       61 ggcgggcgcg tcacggccgc tcccggaacc ggcgctccag aaacggttgg tgcaccgcgt
      121 cggttttgta tgttcccgtc aaagcgaaga ggacgatgtt cgtgaaggtt ttttgggctt
      181 cccgacgttg gccttccccg ccgggttcgc aggggtagag gggctggccg acgcggtccc
      241 gggccagggc gcccagaacg tcgttgcgat cgtagacggc gaccagccgg tccccggccc
      301 aaagccccca gagtcgaccg tcggtttgcc gtcgaccgcc cgcccggggc agaaggaaaa
      361 aagaccggaa gagcgcgtgg tccgccggca cgggccgcca ggtttccgag ggaaacagcc
      421 gggaaggcag ggcctgggcg gcgcggtcaa aggggttgga ggccccgcct tccgtgttgt
      481 cgatccagag aaatcccccc gcccgaaggt gctcccgcag tcgggccacc tccccgtcgg
      541 tcaattccgg atcgccccgg cccgccaaaa ccaaaaaggg cgattcggga agagcgtcgc
      601 cgtccagcga aacgacgcgg cgggcgggcc aggggtcgag actggtggcg tttcgaagga
      661 gcgccgccgc cgacgaccac gcttcgggat aggggtccca gtcgccggcg tggcgaatct
      721 gggcgaaaac caaccggtcg ccgggggcgt cgacctccgc cgcgcgcgga ccgaccgcca
      781 gggccaggac cgcgaggacc ccggccgccc gccgcctcat cggacccgcc ggaaaaaaag
      841 catgagggtt tccaggacaa aaagaacgag aagtccgcgc cgcaacagcg gtcgaagggg
      901 cgatccggcc aatgtccggg acaaatcgtc gcccgccccg atccaaacgg tgcggcccgg
      961 accgaaccac cgttccggga cgtcccgggg aacgcgggcg ggattgccct cctccgcgtc
     1021 ccgcgacgcg ttgaccgcga agaccgtctc ccctcccgac gggaattgaa ggcggtaaaa
     1081 ccccggaagt tccaggggcc ccgcgcgaaa ttcgccggcg cggcccgtca accgctcgct
     1141 ccgcccgtcc gggcgccgaa gcaacgcggc gacggcgccg gagcgacggc cggtccagac
     1201 gccgcccacg gtttccggcg cgggagccga ggcggcttcg g
//
LOCUS       DAOQLS010000725         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_98314_length_1239_cov_3.719595, whole genome shotgun sequence.
ACCESSION   DAOQLS010000725 DAOQLS010000000
VERSION     DAOQLS010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98314_length_1239_cov_3.719595"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="dnaX"
                     /locus_tag="PK362_11025"
     CDS             complement(<1..971)
                     /gene="dnaX"
                     /locus_tag="PK362_11025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNC75399.1"
                     /translation="MPYLVLARKFRPQLFEELVGQEHVTQTLTNALKSQRLAHAYIFS
                     GPRGCGKTTTARILAKALNCVKGPTATPCGECPQCKEIAAGSSTDDVLEIDGASNRGI
                     DQIRELRDTVKYAPARSRYRVIIIDEAHQISKDGFNALLKTLEEPPPHVVFMMATTEA
                     QKIPETILSRCQRFQLRAISPADIADQLRKIISADGLTIEDAALDEVARAAHGSLRDA
                     LSLMDQVIAYAPTGATAEDVRKLLGLLPRELVRSFTTTLREESPVKTLQAIDRAVADG
                     FDLGQLTEALVDHFHRLLLWKAGVRDPRAADARDLEVDASAYSVERL"
     gene            1120..>1239
                     /locus_tag="PK362_11030"
     CDS             1120..>1239
                     /locus_tag="PK362_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC75400.1"
                     /translation="MKKNGFTLIELMLVVAIIGLLAAIAIPKFANMVVKAREAS"
BASE COUNT          210 a          346 c          451 g          232 t
ORIGIN      
        1 tccagccgct cgacggaata ggcggacgcg tcgacttcca ggtcccgggc gtcggcggcc
       61 cgggggtccc ggacgccggc cttccataga aggagccgat ggaaatggtc caccagggcc
      121 tcggtcagct ggcccaaatc gaaaccgtcg gccacggccc gatcgatggc ttgaagggtt
      181 ttaacgggcg attcctcccg cagggtcgtc gtgaagctcc gaacgagctc ccggggcagg
      241 agccccaaca gttttcgaac gtcctcggcg gtggcgcccg tcggggcgta ggcgatcacc
      301 tggtccatga gcgacagggc gtcccgcagg gacccgtggg cggcccgggc cacttcgtcc
      361 agggcggcgt cctcgatggt caggccgtcg gcggagatga ttttgcgcag ttgatcggcg
      421 atgtccgccg gggaaatggc gcgcaattgg aagcgttggc accgggagag gatggtctcc
      481 ggaatcttct gggcttcggt cgtggccatc atgaagacca cgtggggggg cggctcctcc
      541 aaggttttta agagggcgtt gaatccgtcc ttggaaatct ggtgggcttc gtcgatgatg
      601 atgacgcggt agcgggagcg ggccggggcg tatttgacgg tgtcccgtag ttcgcggatt
      661 tggtcgatgc cccggttgga ggcgccgtcg atctccagca cgtcgtccgt ggaagagccc
      721 gcggcgattt ccttgcactg ggggcattcc ccgcagggcg tggccgtggg gcccttcacg
      781 cagttcaggg ccttggcgag gatgcgggcg gtggtggtct tgccgcaacc ccgggggccg
      841 gaaaaaatat aggcgtgggc caggcgctgg cttttgagcg cgttggtcag ggtttgggtg
      901 acgtgttcct ggcccaccag ctcttcaaaa agctggggac ggaactttcg ggcaaggacg
      961 agatagggca tggcggggcc tcggaaaaga acggaaagga ctttatcaat ttccgtcgga
     1021 gcggtcaatc gaaggcccgg ggcgcttcgg ccggcgggcg ggtccgaatt cccttgcaaa
     1081 agcgggggga gggggatacc cttaatcggg gacctttcga tgaaaaaaaa cgggttcacg
     1141 ctgattgagc tcatgctggt ggtggccatc atcggcctcc tggccgccat cgccattccc
     1201 aaattcgcca acatggtggt caaggcccgc gaggcgtcc
//
LOCUS       DAOQLS010000726         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_98360_length_1239_cov_2.733953, whole genome shotgun sequence.
ACCESSION   DAOQLS010000726 DAOQLS010000000
VERSION     DAOQLS010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98360_length_1239_cov_2.733953"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PK362_11035"
     CDS             complement(<1..1012)
                     /locus_tag="PK362_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75401.1"
                     /translation="MKYFRALAPVVPILMSACAKPDPTKFNFVARNLAADGRAYGVAV
                     GPGGDVYQAGSFVAGRYLSGGPINGSLWLGRWSPDLRLLNARSIDGLFPGQDAAYGVA
                     VDPAGNVYAVGFVAVLSGGEAPWVGKFDRNLNPLGSFTGSPAVEGRRKYSQTIAVGSE
                     ENIFLGGWIGHRPSEANEVLGENVAHYGRGQIPWLAKMSPALVPLKEIFRDDVGSGLQ
                     RVCALAPDPAGGVYAAGAVSSRSTSGEKRWWVAHYDRNLALLAEYASPAGGDFEARGA
                     VLAGDGSLWIAGCEGQHGFVAHFSADLKPLSQRTFEPKPLRWAPVVRPLRLALAPKGL
                     WLS"
     gene            complement(1032..>1239)
                     /locus_tag="PK362_11040"
     CDS             complement(1032..>1239)
                     /locus_tag="PK362_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75402.1"
                     /translation="ALFEAVHRRAAEVLYWHLDGRFLGTTRHFHQQALDLTAGVHELT
                     LVDREGHRLRRRFEVLSAGRPGAR"
BASE COUNT          240 a          424 c          385 g          190 t
ORIGIN      
        1 ccgatagcca gagtcccttc ggcgccaggg cgagacgcag gggccggacc accggcgccc
       61 aacgaagggg cttgggttca aaggttcgtt gggacaaagg cttgagatcg gcggagaagt
      121 gggcgacaaa accgtgttgt ccttcgcacc ccgcgatcca aagactgccg tccccggcca
      181 agaccgcgcc ccgggcttcg aagtctccgc ccgcgggact ggcgtattcg gcgaggagag
      241 cgaggtttcg gtcgtagtgg gccacccacc agcgcttttc ccccgaggtg gaacggctgg
      301 aaaccgcccc cgccgcgtaa acgcccccgg ccgggtccgg cgcgagggcg cacacccgtt
      361 ggaggccgct tccgacgtcg tcccggaaaa tctctttcaa cggcacgagc gctggcgaca
      421 ttttcgccag ccaggggatt tgaccccggc cgtagtgggc cacgttttcg cccagaacct
      481 cgttggcctc ggacggacgg tggccgatcc aaccgcccag aaaaatattt tcctccgagc
      541 ccacggcgat ggtttggctg tattttcgcc ggccctccac cgccggcgac ccggtgaaag
      601 acccgagggg gttcaaattt cggtcaaact tccccaccca cggggcttcg cccccgctca
      661 gcacggcgac gaacccgacg gcgtaaacgt ttccggcggg gtccacggcc acgccatagg
      721 ccgcgtcctg tcccggaaac aatccgtcga tggaacgggc gttcaacaat cgaaggtccg
      781 gcgaccaacg gccgagccaa agggacccgt tgatcggacc gccggagaga tagcgcccgg
      841 ccacgaaact tcccgcttgg taaacgtcgc cgccggggcc caccgcaacc ccgtaggcgc
      901 gcccatcggc cgcgaggttt cgggcaacga aattaaattt tgtcggatcg ggtttggcgc
      961 aagccgacat caagatcggg acaacggggg ccagcgcgcg gaaatacttc atcgattcct
     1021 ccacaggtct ttcaccgggc cccggggcgg ccggcgctca gaacttcaaa ccgccgacga
     1081 aggcggtgcc cctcccgatc cacgagggtc agttcgtgaa cccccgcggt caaatccagg
     1141 gcctgttggt gaaagtgccg ggtcgttccc aaaaaccgcc cgtccaaatg ccaataaagc
     1201 acttcggcgg cccgtcggtg gaccgcctcg aaaagcgcc
//
LOCUS       DAOQLS010000727         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_98943_length_1235_cov_2.900000, whole genome shotgun sequence.
ACCESSION   DAOQLS010000727 DAOQLS010000000
VERSION     DAOQLS010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98943_length_1235_cov_2.900000"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..700)
                     /locus_tag="PK362_11045"
                     /pseudo
     CDS             complement(92..700)
                     /locus_tag="PK362_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
BASE COUNT          249 a          333 c          381 g          272 t
ORIGIN      
        1 ggtcatcgac ccgttcaaga cccgcccata aaaaagaatc ccccctttcg gggggattct
       61 tttttagttt gaaaagccga cgaaccgcga tttagaactt gtacgcgagc ccgaccgaga
      121 ggttggtcac ggacgtggta tcgccgaaac gggtgttttt gtcgttgtct ccgtgggtgt
      181 gttggcggaa gttgaccgtt tcgctttgca gacgcgccac gaggtggggg gagagcatcg
      241 tttccacgcc caccgcgtag gccatgccga aataccatcg accgacggcg taggggtgcg
      301 gtgtgccgcc cgtccagccc gtgtcgtcgc tcatgaagtc caccgcggcc cgggccggac
      361 ccgcgcccag gtaaatcatc gcgttgtcca cgaccacccc ggcgcgcact ttgagcgtct
      421 gaagggattt gatggtggtt tgttggtagg gaatgggggc gcaacccgtg cagcccatct
      481 cggaacggtc gttgttgtcc aaacgaagag tttcaacttc cacgccgtag aggaagctcc
      541 gcttttggcg ggcgtatccc aggtagaacc ccttggaccg ttcggtgtcc gaaattccgt
      601 tttgttcctg gttgtcccag aaatcgttct tgtcggcgta atccgtaacg gaacgggccg
      661 agcccagttg caagcccaag taagcgccgc tccaatcgtc cgtcgctcgc gcggctccgg
      721 ccgattagaa cgcggcaacg atccccgcca ccccaatcgc atttttcatt ttcattcggt
      781 gatgtctccc ttttcgatgt aaacaaagat tcaaatgaat atacggtggg aactgtaata
      841 tttcaaggct ttggcgtaaa ggcgttttcg cctttgggaa ctcgggcctc ctacgtcttc
      901 cgagcgcggg attcgcgcgc aaattacgaa ggcgcggtgc gcttggggaa atcgggaatg
      961 gccaaaagcg cgccttccca ctatacttta gaggccgccg gtcgcatcgt gcggttatta
     1021 aaggacggtc atcggcggat aaaaaggaga gacggatgaa gaccattggc tggatcggcc
     1081 ccttggcggg gaggggcgcc cccgccttcg cgccggggtt aaaatgccga tgaaaaagag
     1141 tggatggtcc gttgtggttt ggacgacggc gatggcgacc ctggcggtcg gtttgagcgg
     1201 aggcgataaa atgaaatatc cggtggcgaa agtca
//
LOCUS       DAOQLS010000728         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_98991_length_1235_cov_2.122034, whole genome shotgun sequence.
ACCESSION   DAOQLS010000728 DAOQLS010000000
VERSION     DAOQLS010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98991_length_1235_cov_2.122034"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1235)
                     /locus_tag="PK362_11050"
     CDS             complement(<1..>1235)
                     /locus_tag="PK362_11050"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC75403.1"
                     /translation="TMIQAHRFDGMVCIPNCDKIVPGMIMGALRVDIPTIFVSGGAMA
                     AGKTPEGQTIDLISVFEGVGAYQAGKIDDQRLTTLEKFACPTCGSCSGMFTANSMNCL
                     MEVLGLALPFNGSALAKTDEREDLARTAAAQIMTLIEMGLTPRQLITPEAIDDAFALD
                     MAMGGSTNTVLHTLAFTHEAGIDYPLERINKVADKVPHLCKVSPAGKWHMEDVHRAGG
                     IPAILNEIAVDGNTLHLDRMTVTGKTMRENIAGKRNYDTDVIRTRETAHSPVGGLAVL
                     FGNLAPDGAIVKVGGVAPAMMRHTGPARIYESEEEAMRGILNREVQEGEVVVVRYEGP
                     RGGPGMQEMLSPTSAIMGMGLGEKVALITDGRFSGGTAGACIGHVSPEAAEGGPIGLL
                     KDGDRLRIERRRGGTGGEQ"
BASE COUNT          209 a          368 c          396 g          262 t
ORIGIN      
        1 cctgctcgcc gcctgttcct ccgcgccgcc gttcgatgcg aaggcgatcg ccatccttga
       61 gcaagccgat cgggccgcct tccgccgctt ccggggaaac gtgaccaatg cacgcaccgg
      121 ctgtgccgcc gctgaagcga ccgtccgtga tcagggcgac tttttcaccc aaccccatac
      181 ccatgatggc ggaggtgggg gagagcattt cctgcatccc cgggccgccg cgggggcctt
      241 cgtaccggac gacgacgact tcgccttcct gcacttcccg gttgaggatg ccgcgcatcg
      301 cttcttcttc gctttcgtaa atgcgcgccg ggccggtgtg acgcatcatc gccggagcca
      361 cgccaccgac tttgacaatc gccccatcgg gggccagatt cccgaacaga accgccaacc
      421 cgccgacagg ggaatgggcc gtttcgcggg tgcggatcac gtcggtgtcg taattccgct
      481 tgcctgcgat gttttcgcgc atggttttcc cggtcacggt catccggtcg aggtgtaatg
      541 tgttcccatc cacggcgatc tcgttcaaaa tcgcggggat gccacccgcg cggtgaacgt
      601 cttccatgtg ccattttccg gcggggctga ctttgcacag gtggggcact ttgtcggcga
      661 ctttgttgat gcgttcgagg gggtagtcaa tcccggcctc gtgggtgaag gcgagggtgt
      721 gcaggacggt gttggtggag ccgcccatcg ccatgtctag cgcgaaggcg tcgtcaatcg
      781 cttcgggggt gatgagctgg cgaggggtga gacccatctc gatcagggtc atgatttggg
      841 cggcggcggt acgggcgagg tcttcacgtt cgtctgtttt ggcgagtgca gacccattaa
      901 aaggcaacgc caacccaagc acttccatga gacagttcat tgagttggcg gtgaacatgc
      961 cggaacacga tccacaggtc ggacaggcga atttttccag ggtggtgaga cgctggtcgt
     1021 caatttttcc ggcctggtac gcgcccacgc cttcaaagac ggagatcagg tcaatcgttt
     1081 ggccttcggg ggttttgccc gcggccatcg caccgccgga cacgaaaatg gtggggatgt
     1141 ccacgcggag tgcgcccatg atcatgccgg ggacgatttt gtcacagttg gggatgcaca
     1201 ccatgccgtc gaaacggtgg gcctgaatca tcgtt
//
LOCUS       DAOQLS010000729         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_99086_length_1234_cov_3.289228, whole genome shotgun sequence.
ACCESSION   DAOQLS010000729 DAOQLS010000000
VERSION     DAOQLS010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99086_length_1234_cov_3.289228"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PK362_11055"
     CDS             complement(<1..1216)
                     /locus_tag="PK362_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC75404.1"
                     /translation="MKVTPSHDATDFEIGARHHLPHEMVIGFDGKMTARAGRYAGLSV
                     KDAREKVVADLEAKGLLVKIDDHRHAVAVCYRCATAIEPLESSQWFLKTAEMAERAAR
                     ATEEGRVRIHPESWAKPYLAWLHGNRDWCVSRQIWWGHQVPVWYCLSCGDYSDEQMRR
                     IRADKSLSSEITKSLLRGSAKRPIASAEPLATCPDCGGRDLLQDPDVLDTWFSSGLWP
                     LTTLGWPADTEDLKHFYPTAVLATGHEILYLWVARMVMMGLALRNDIPYKDVFIHGIV
                     RDKQGRKMSKSLNNVIDPLDIMKKFGTDALRFALVAQASPGRDMQLSDDSFVGARNFA
                     NKVWNASRFVMMNLRGHRKIDLPWAERDLADRWIADRFRRAAAEADRFFDQFDPAQAA
                     RTLYAFFWNDFCD"
BASE COUNT          217 a          380 c          416 g          221 t
ORIGIN      
        1 aatcgcaaaa gtcgttccag aaaaaggcgt aaagggtccg cgccgcctgg gccgggtcga
       61 attggtcgaa gaagcggtcg gcttcggccg ccgcccggcg gaaacggtcc gcgatccagc
      121 ggtcggccaa atcccgctcc gcccagggaa ggtcgatttt tcggtgcccc cgcaggttca
      181 tcatcacaaa gcggctggcg ttccacacct tgttggcgaa atttcgggcc cccacaaaac
      241 tgtcgtcgga cagctgcatg tcgcgcccgg gggacgcctg ggccacgaga gcgaagcgca
      301 gggcgtcggt gccgaacttc ttcatgatgt ccagggggtc gatgacgttg ttcagggact
      361 tggacatttt ccggccctgt ttgtcccgga cgatgccgtg gatgaaaacg tctttgtagg
      421 ggatgtcgtt ccgcagggcg aggcccatca tcaccatccg ggccacccac agataaagga
      481 tttcgtggcc cgtcgccaaa acggcggtgg ggtaaaagtg cttcaaatcc tcggtgtccg
      541 cgggccagcc cagggtggtc aggggccaga gccccgagga gaaccaggtg tccagcacgt
      601 ccggatcctg aagcaggtcc cggccgccgc aatcggggca ggtggcgagc ggttcggccg
      661 aggcgatggg ccgtttggcg gagccccgca ggagggattt cgtgatctcg gaggagaggg
      721 acttgtccgc ccgaatgcgc cgcatttgtt cgtcggaata gtccccgcag gacaggcagt
      781 accagaccgg cacctggtgg ccccaccata tttgccggga cacgcaccag tcgcggttgc
      841 cgtggagcca cgccagataa ggtttcgccc aggattcggg gtgaatgcgg acccgaccct
      901 cctcggtggc ccgggccgcg cgctccgcca tctcggcggt cttgaggaac cattggctgg
      961 attccagggg ttcgatggcc gtggcgcagc ggtagcaaac ggccacggcg tgccggtgat
     1021 cgtcgatttt gaccaacagc cccttggcct ccaggtccgc cacgactttc tcccgggcgt
     1081 ctttgacgga gagcccggcg tagcgccccg cgcgggcggt catttttccg tcgaaaccga
     1141 tcaccatttc gtggggcaga tggtggcgcg cgccgatctc gaaatccgtc gcgtcatggg
     1201 acggggtgac tttcaccgcc ccggtgccga aaga
//
LOCUS       DAOQLS010000730         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_99377_length_1232_cov_3.384877, whole genome shotgun sequence.
ACCESSION   DAOQLS010000730 DAOQLS010000000
VERSION     DAOQLS010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99377_length_1232_cov_3.384877"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK362_11060"
     CDS             <1..543
                     /locus_tag="PK362_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC75405.1"
                     /translation="ATPQLPSEGDDHKTGAAALGELCHAVLAEWDFEGAGLDAVFSAV
                     LHRFQNERRLTVWADLAPEARRMLAAFSESPAARSLRRGEILLRESPVLFPRGGKIVR
                     GSLDILYRLDGRNILADFKTDRVEPGEEKDRARSYEGQGEAYLEGVRRSLGLDCEFEV
                     IFIRTGGAVRVLKAKTIDNK"
     gene            580..1173
                     /locus_tag="PK362_11065"
     CDS             580..1173
                     /locus_tag="PK362_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75406.1"
                     /translation="MREKAFGTHDIARICHVTPPTVGRWIEEGLLPSFTTGGGHRRVW
                     DEDLLVFLRAHNIPVPPELVAEGPPRVLVVDDEPRLRQMILRILKKAYPAAEFHEAEN
                     GFDAGKMISELIPTLVILDLRLPGVDGLKVCESVKNDDRLKQVRILTMTGFDVERSRE
                     ETREAGADGFVAKPFKPDVFLDEVRRLLPVPAASKVA"
BASE COUNT          235 a          361 c          399 g          237 t
ORIGIN      
        1 gcgacgcctc aacttccctc ggaaggggac gatcataaaa ccggggccgc ggccctgggg
       61 gagttgtgtc acgcggtgtt ggcggaatgg gatttcgagg gcgccgggct cgacgccgtg
      121 ttctccgcgg tcctccaccg gttccaaaac gagcggcggt tgaccgtttg ggcggacctg
      181 gctcccgagg cgcggcgaat gctcgccgcg ttttcggaat cccccgcggc gcggtcgctc
      241 cggcgcgggg aaattctttt gagggaatct ccggtgcttt ttccccgggg cggaaaaatt
      301 gtgcggggct ccctggacat cctctatcgg ttggacgggc gaaacatcct ggcggacttt
      361 aaaacggacc gggtggagcc gggggaagag aaggaccgcg cccgaagcta cgaaggtcaa
      421 ggggaggcct atctcgaggg ggttcggcgc tcccttggtt tggattgtga attcgaggtg
      481 atttttattc gaaccggtgg cgcggtccgc gttttgaagg caaaaaccat tgacaacaaa
      541 tgatacttag ggtatgaata agggtcagga aaggttccta tgcgtgaaaa agcgttcggc
      601 acacacgaca tcgcccgcat ttgccatgtg acgcccccca ccgtgggccg gtggattgaa
      661 gaaggtctct tgccgtcttt cacgacgggg ggaggccatc gacgcgtttg ggacgaggac
      721 cttctcgttt tccttcgggc gcacaacatc ccggtcccgc cggaactggt cgccgaaggg
      781 ccgccccggg tcctggtggt ggacgacgag ccgcgcctgc gacagatgat tcttcggatt
      841 ttaaagaaag cctaccccgc cgcggaattt cacgaggccg aaaacggctt tgacgccggc
      901 aaaatgattt cggagttgat ccccaccctg gtcatcctgg atttgcgcct gccgggcgtg
      961 gacggactca aagtctgcga aagcgtcaaa aacgacgacc gcctgaaaca ggttcgaatc
     1021 ctgaccatga cggggttcga cgtcgaacgg tcccgggagg aaacccggga ggccggcgcc
     1081 gacgggttcg tggcgaaacc ctttaaaccc gacgtcttcc tggacgaagt ccgccggctt
     1141 ctgcccgttc ccgccgccag caaagtcgct taacctcttt cccctttccc aaaagcgaac
     1201 ggcggggcct ccgtcgccgg aagcgcccgc cg
//
LOCUS       DAOQLS010000731         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_99678_length_1230_cov_5.101277, whole genome shotgun sequence.
ACCESSION   DAOQLS010000731 DAOQLS010000000
VERSION     DAOQLS010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99678_length_1230_cov_5.101277"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..440
                     /locus_tag="PK362_11070"
     CDS             117..440
                     /locus_tag="PK362_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75407.1"
                     /translation="MKWIPEAHCPKCKGPITFFDVLGIVTPFQCIYCVSCGEMVFLKH
                     RWTFLSVAVLAGVLMVLFSIEVVTKSLASMMSTFVVSLISLVALEIVMTGYVVKKNDL
                     VVRRP"
     gene            complement(496..>1230)
                     /locus_tag="PK362_11075"
     CDS             complement(496..>1230)
                     /locus_tag="PK362_11075"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC75408.1"
                     /translation="DGVFHQAAITDTTVMDEKLMRDVNVHAFVRILEAAHRTGTPKVV
                     YASSAATYGKGPVPNREDQTPAPANIYGVSKVEMEQAAKAFTDGHPAVQAVGLRYFNV
                     YGPLEFHKKAAASMIYQLYLQIAAGKSPRIFKWGEQYRDFVYVKDVVAANWKAWEYAG
                     NGVFNVGTGLGTTFNDLIAHLGRALGVTPKTEYFDNPYGFYQEATRADMTRSNTKMGF
                     KAAFPPADGIADYVHFLKTRTSPLPK"
BASE COUNT          214 a          326 c          397 g          293 t
ORIGIN      
        1 ggggtcccgg ggaacgattc gttccccggg acctttttta ttgggagagg cgcaacgtcc
       61 tttcgagagg gaaccgcctt gacaaacagg actttcgggc atagcctttg ggtgtcatga
      121 aatggattcc cgaagcccac tgtccgaaat gcaaaggccc catcacgttt tttgacgtgt
      181 tgggcattgt gacgcccttt cagtgcattt attgcgtgag ttgcggggag atggttttct
      241 taaaacaccg gtggacgttt ctgagcgtgg cggttttggc gggggtgctc atggttctgt
      301 tttccatcga ggtggtcact aaaagcctcg cttccatgat gtcgacgttt gtcgtttcgc
      361 tgatctccct ggtcgcgctg gaaatcgtca tgacgggata cgtcgtcaaa aagaacgatt
      421 tggtggtgcg gcggccctaa gcccagaatc gaatttccgt cggagataaa cgaaccccgc
      481 cttcggcggg gttttttatt tggggagggg ggaggtccgg gttttaagga agtggacgta
      541 atccgcaatg ccgtcggccg gcgggaaggc ggctttgaag cccattttgg tgttcgaacg
      601 ggtcatgtcg gcccgggtgg cctcctggta gaacccgtag ggattgtcga aatactcggt
      661 tttcggcgtc acgcccaggg cccggcccag gtgggcgatg aggtcgttga aggtcgtgcc
      721 caggccggtc cccacgttga acacgccgtt gcccgcgtat tcccaggcct tccagttggc
      781 ggccacgacg tccttcacgt acacgaaatc ccggtactgc tcgccccatt tgaatatccg
      841 gggggatttg ccggcggcga tttgaaggta gagctggtaa atcatgctgg ccgcggcctt
      901 tttgtggaat tccagggggc cgtaaacgtt gaaataccga aggccgacgg cctggaccgc
      961 cgggtgaccg tcggtgaacg ctttggccgc ctgttccatc tccaccttgc tcacgccgta
     1021 gatattggcc ggggcgggcg tttggtcctc ccggttgggc accggaccct ttccgtaggt
     1081 ggcggcgctg gaagcgtaaa cgaccttggg agtgccggtg cgatgggcgg cctccaaaat
     1141 tcgcacgaag gcgtgcacgt tgacgtcccg catcaacttt tcatccatca cggtggtgtc
     1201 ggtgatggcc gcctggtgga acaccccgtc
//
LOCUS       DAOQLS010000732         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_99820_length_1230_cov_1.674894, whole genome shotgun sequence.
ACCESSION   DAOQLS010000732 DAOQLS010000000
VERSION     DAOQLS010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99820_length_1230_cov_1.674894"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1230
                     /locus_tag="PK362_11080"
     CDS             <1..>1230
                     /locus_tag="PK362_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC75409.1"
                     /translation="LSSIQNLGAMNVFCTDKTGTLTMDEVVLERHCDVILQEDEAVLT
                     LAYLNSVHQTGLKNLLDRAILKHHDEVRALGEKVDEIPFDFSRRILSVVVSQEGRHRL
                     IAKGAPEEIFKRCARYELDGEIFPMEDLILRDLQEEHDALSRDGYRVLAIAYRDMAEP
                     KTAYGKADETDLVLRGYLAFLDPPKPSAKAALAALRRRGVACKGLTGDNALVTQKIAR
                     EVGLPVDGMLTGDELAALSDEALEPRLASCTIFARLAPLQKERVIRLLQKTGATVGYL
                     GDGINDAPALKAADVGLSVDNAVDVARESADIILLRKNLNVLEAGIVEGRRTFANILK
                     YIRMGASSNFGNMISMTGASLVLPFLPMLPIQVLLNNFLYDLSQVALPTDTVDPEALD
                     NPRPWDVAAIQRFMFIFG"
BASE COUNT          243 a          380 c          365 g          242 t
ORIGIN      
        1 ctgtcctcca ttcaaaatct gggcgccatg aacgttttct gcaccgacaa aaccggcacc
       61 ctcacgatgg acgaggtggt gctggagcgg cactgcgacg tgattctcca ggaagacgag
      121 gcggttttaa ccctcgccta cttaaacagc gtccaccaaa ccggactgaa gaacctgctc
      181 gatcgggcca tcctcaagca tcacgacgag gtccgggcgc tgggggaaaa agtggatgag
      241 atcccgtttg atttttcccg gcggatcctg tccgtcgtcg tgtcccagga ggggcgccac
      301 cgcctcatcg ccaaaggggc tccggaagaa atttttaaac ggtgcgcgcg ctacgagttg
      361 gacggcgaaa tatttcccat ggaagatctc attctccggg accttcaaga agagcacgac
      421 gccttgagcc gcgacggtta ccgcgtgttg gccatcgctt atcgggacat ggcggaaccc
      481 aaaaccgctt acggcaaagc cgacgagacc gacctggtct tgcgggggta tttggcgttt
      541 ctggaccctc ccaaaccctc cgccaaagcg gcgttggcgg ccttgcgccg gcggggcgtc
      601 gcctgcaagg ggttgacggg cgacaacgcg ctggtcactc aaaagatcgc gcgggaggtg
      661 gggttgccgg tcgacggcat gttgacgggc gacgaattgg cggcgctttc cgacgaggcc
      721 ttggaacccc ggttggcctc ctgcacgatt ttcgcccgac tggcccctct ccaaaaagag
      781 cgggtgatcc gcctcctcca aaaaaccggg gcgacggtgg ggtatttggg cgacgggatc
      841 aacgacgcgc cggccttgaa agccgccgac gtgggcctct ccgtcgacaa cgccgtggac
      901 gtggcgcggg aatcggccga catcatcctg cttcgcaaaa atctcaacgt gctcgaagcc
      961 gggatcgtgg aaggccgccg caccttcgcc aacatcctca aatacatccg catgggggcg
     1021 agttccaatt tcggcaacat gatcagcatg accggcgcca gcctcgtgtt gccctttttg
     1081 cccatgctgc ccatccaggt cctgttgaac aacttccttt atgatctttc ccaggtggct
     1141 cttccgaccg acacggtgga ccccgaagcc ttggataatc cccgcccttg ggacgtggcc
     1201 gcgattcaac ggttcatgtt cattttcggc
//
LOCUS       DAOQLS010000733         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_99860_length_1229_cov_4.431857, whole genome shotgun sequence.
ACCESSION   DAOQLS010000733 DAOQLS010000000
VERSION     DAOQLS010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99860_length_1229_cov_4.431857"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PK362_11085"
     CDS             complement(<1..471)
                     /locus_tag="PK362_11085"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HNC75410.1"
                     /translation="MRTIARATSFLSAVLLALTFGCAKPPTIEITDAENAVNAAEQAG
                     ALEYATDNFQAAKDALSDAKTKVEAKDYKNALASALDAKAKAEAAQAAVESGKAAAKA
                     EAQAGIDAIEKEVTALGDKAKKLKGAAAGEVKTALNALEADWTKVKEDFLAANFT"
     gene            complement(542..>1229)
                     /locus_tag="PK362_11090"
     CDS             complement(542..>1229)
                     /locus_tag="PK362_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC75411.1"
                     /translation="GISRNRRDYFSNVRINNGLTAGLGVGTQWNFADLAALSFELSLR
                     HVDKASPSGKAAKIADAGILVSFYLPEGWVPLIPKEELSLEDLETPLDSAPGQVDEAL
                     QMQGEINRVLQQIEDGQTPPVTFEAGNSIMLTTSYEALDNIGAILRRHPEMKVRVYGF
                     VEEEFVGGVVRGETLALARAEAVRTYLNQNFRLPDGTLISAGLPPLPAVLDGQEPPPP
                     ARHISFEVLP"
BASE COUNT          193 a          343 c          419 g          274 t
ORIGIN      
        1 ggtgaagttg gcggccagga agtcttcctt caccttggtc caatcggctt ccagggcgtt
       61 caaggcggtt ttcacttccc cggcggccgc gcctttgagt ttcttggctt tgtcgccgag
      121 ggcggtcact tctttttcga tggcgtcgat gccggcctgc gcttcggctt tggcggcggc
      181 tttgccggat tcgacggcgg cttgggcggc ttccgctttg gccttcgcgt ccagggccga
      241 ggccagggcg ttcttatagt ccttggcttc gactttcgtc ttggcgtcgc tcaaggcgtc
      301 cttggcggct tggaaattgt cggtggcgta ttccagggcg ccggcttgtt cggcggcgtt
      361 gaccgcgttt tccgcgtcgg tgatctcgat cgtcggcggt ttggcgcagc cgaaggtcag
      421 ggccaacagg acggcgctca aaaacgatgt tgcacgggcg atggttctca taacttcctc
      481 cttggaattt ggaaaggggg gtaaaaggac ggcttgatta tccgattttt tcatccgccg
      541 atcaaggcag gacttcaaac gaaatatgcc gcgcgggagg cggcggttcc tgcccgtcca
      601 aaacggcggg gagcggcgga agtcccgcgc tgatgagggt gccatccggg agacggaaat
      661 tctggttcag gtaggtgcgc acggcttcgg cccgggccag ggccagggtt tcgccccgga
      721 ccacgccgcc cacgaattcc tcctcgacaa agccgtatac gcgaactttc atctccggat
      781 ggcggcgcag gatggcgccg atgttgtcca gggcttcgta ggacgtggtc agcatgatgg
      841 agttcccggc ttcgaaggtg acggggggcg tctggccgtc ctcgatttgt tgaagcacgc
      901 ggttgatttc cccctgcatt tgaagggcct cgtccacttg gccgggggcg gaatccaggg
      961 gcgtttcgag gtcttccagg gaaagctctt ccttgggaat caagggaacc cagccttccg
     1021 ggagataaaa agagacgagg atcccggcgt cggcgatctt ggccgccttg ccggaggggg
     1081 aggccttgtc cacgtggcgg aggctcagtt cgaagctcaa ggcggccaag tccgcaaaat
     1141 tccattgggt gcccacgccc aggccggcgg tcaacccgtt gttgatgcgc acgttggaaa
     1201 agtagtcccg gcggttgcgg ctgatgccg
//
LOCUS       DAOQLS010000734         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_99874_length_1229_cov_3.635434, whole genome shotgun sequence.
ACCESSION   DAOQLS010000734 DAOQLS010000000
VERSION     DAOQLS010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99874_length_1229_cov_3.635434"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PK362_11095"
     CDS             complement(<1..1146)
                     /locus_tag="PK362_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNC75412.1"
                     /translation="MKRRNQILILGVAAVAGVFGLPPVRSAVDTTYEQIKLLVDVLQY
                     IREQYVESVDQKAIVYGAASGMTRALDPFSQFMEPEAHKEMKTETQGQFGGLGIRIAV
                     RDGWLTVITPMPDTPAYRLGILPGDKIVKIEDEVTQGLSVEDAVTRLRGKPGTKVSLT
                     IAREGDKEPRVFSITREVIKIQSVKSALLDGGIGYLRLSEFIETSNADLLKAGRELKS
                     KGMNSLILDLRNNPGGLLTSAVDVTKLFLGDNKLIVYTQGRAQPRADYLADAQAPYGN
                     LPLAILVNGGSASASEIVTGALQDQRRAIIVGSETFGKGSVQSVIPLEDGSALRLTVA
                     KYYTPSGRSIHRDEKTKKGGITPDIVIAVPRETEAKLQSQSEEVYAKD"
BASE COUNT          208 a          393 c          364 g          264 t
ORIGIN      
        1 gtccttggcg taaacctcct cggattggga ttgaagcttc gcctcggttt cgcgcggcac
       61 ggcgatcacg atgtccgggg tgatgccgcc cttttttgtt ttttcgtcgc ggtgaatgga
      121 ccgcccggac ggcgtgtaat atttggcgac ggtcaaccgg agggccgaac cgtcttccaa
      181 cggaatcacg gactggacgc tccccttccc gaacgtctcc gacccgacga tgatggcgcg
      241 ccgctggtcc tgcagcgccc ccgtcacaat ttccgaagcc gacgccgacc cgccgttgac
      301 caggatggcc aggggcaggt tcccgtacgg cgcctgggcg tcggccaggt aatccgcccg
      361 gggctgggcg cgtccctggg tgtaaacgat cagtttgttg tccccgagga acaatttcgt
      421 cacgtcgacg gccgacgtca gcaagccgcc cgggttgttc cgcaaatcca ggatgaggga
      481 gttcatcccc ttgctcttga gctcccggcc cgccttcaga agatcggcgt tcgaagtctc
      541 gatgaattcg ctcaaccgca gatacccgat cccgccgtcc aacagggcgg acttgacgct
      601 ttgaattttg atcacttccc gggtgatgga gaagacccgc ggttccttgt cgccctcccg
      661 ggcgatggtc agagacacct tggttccggg tttcccccgc aggcgggtca ccgcgtcctc
      721 gacgctcaga ccctgggtga cttcgtcttc gattttaacg atcttgtcgc cgggcaaaat
      781 ccccaaccgg taggccgggg tgtccggcat cggcgtaatg acggtcaacc acccgtcgcg
      841 gacggcgatg cggatgccca accccccgaa ctggccctgg gtttcggttt tcatttcctt
      901 gtgggcttcc ggttccatga attggctgaa ggggtccagg gcccgcgtca tgccggacgc
      961 cgccccgtaa acgatggctt tctggtcgac cgattccacg tactgctcgc gaatgtattg
     1021 cagaacgtcc accaagagtt tgatttgttc gtacgtggtg tccacggccg atcgcacggg
     1081 gggcaacccg aaaacgccgg ccacggccgc gacgcccaga attaaaattt gatttcgcct
     1141 tttcatgtcg ttctccttgt cgtggtgatg ccctcagcga acccactcca gggggttcac
     1201 cggcgtgccg cgcacgcgaa gctcaaaat
//
LOCUS       DAOQLS010000735         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_100029_length_1228_cov_4.775789, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000735 DAOQLS010000000
VERSION     DAOQLS010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100029_length_1228_cov_4.775789"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PK362_11100"
     CDS             <1..1179
                     /locus_tag="PK362_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNC75413.1"
                     /translation="VQHNISPETVNCACCGGDAASGDGGDAVPEIGHHLCRRRRGLVP
                     EVLAPILEKRSRYKTLAKTDSPNRLIYKNRANAHKWVLVCCFGYLGFKNARFGKIESH
                     ECVTAWGREILLRAKETVERRGYRVLHGIVDSLWIVGHPGMDYEALRRDIEREAGCPV
                     GLEGVYKWIRFCPSKTDPLAGVPARYFGAFTNGELKVRGLALRRRDTPGLLKEMQEGM
                     LRDLSRAADVEECRGSLPALREKSEEYRYRLKEGRVTPPELAVTFHLSKDPAHYVHDT
                     LSTLAAKQLAASGVPLHAGESVKYIIRAAGDRLKENRSIPLAFLHEGFDYDAQKYGEK
                     LAEAAEEILGGLGPEAPARGKNPPAAPARKRFPKGRRKGKLPVAELFTHPYETERNVP
                     "
BASE COUNT          243 a          394 c          398 g          193 t
ORIGIN      
        1 gtccaacaca acatttcccc cgaaaccgtc aactgcgcct gctgcggcgg ggacgccgcg
       61 tccggggacg ggggcgacgc cgttcccgaa atcggccacc acctgtgccg tcggcggcgc
      121 ggcctggtgc cggaagtcct ggcgcccatt ctggaaaagc gttcccgcta caaaaccctc
      181 gccaaaaccg attcgccgaa ccgcctgatt tacaaaaacc gggccaacgc ccacaaatgg
      241 gtgctggtct gttgtttcgg ttacctggga ttcaagaacg cgcggttcgg gaagatcgaa
      301 tcccacgaat gcgtcacggc ctggggtcgg gaaatcctcc tgcgggccaa ggaaaccgtg
      361 gagcgtcggg gttaccgggt gcttcacggc attgtggact ccctttggat cgtcggacat
      421 cccgggatgg attacgaggc cctccgccgg gacatcgaac gggaggcggg ctgcccggtg
      481 ggcttggagg gagtctacaa atggatccgc ttctgtccct ccaagacgga cccgttggcg
      541 ggggtcccgg cccgttattt cggggctttc accaacggcg agttaaaagt ccgcggtttg
      601 gccctgcgtc ggcgggacac cccgggcctg ttgaaggaaa tgcaggaggg aatgctccgg
      661 gacctgtccc gggccgccga cgttgaggaa tgccggggga gcctgccggc ccttcgggaa
      721 aagagcgagg aataccgcta ccgcctgaaa gaagggcgcg tgacgccgcc ggagctggcc
      781 gtgaccttcc acctgtccaa agaccccgcc cactacgtcc acgacacgct gtcgacgctc
      841 gcggccaagc aactggcggc ctcgggcgtt ccgctccacg ccggggaatc cgtgaaatac
      901 atcattcggg cggccgggga ccgcttgaag gagaaccgct cgatccccct ggcgttcctc
      961 cacgaggggt tcgattacga cgcgcaaaaa tacggggaga aattggccga agcggcggag
     1021 gaaatcctgg gcggtctggg gccggaggcc cccgcccggg gcaagaaccc gccggccgcc
     1081 cccgccagga aacggtttcc caaaggccgg cgaaaaggta aattacccgt cgccgagctt
     1141 ttcacccacc cttacgaaac ggaaaggaac gttccatgat caccaccccg tcgggattgc
     1201 aatacgagga caccgtcacc gggacggg
//
LOCUS       DAOQLS010000736         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_100279_length_1227_cov_2.225256, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000736 DAOQLS010000000
VERSION     DAOQLS010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100279_length_1227_cov_2.225256"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..>1227)
                     /locus_tag="PK362_11105"
     CDS             complement(116..>1227)
                     /locus_tag="PK362_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNC75414.1"
                     /translation="FGGSEPRKVTQGEMMNREVLAKSLVINRALRQGEPITADMIDIR
                     SPGRGLAPYRKNELVGRAARRDFNPGDFFYPTDVEAPAAEPRPYRFRRPFGIPIRYHD
                     LNEMAGRSNFDLLEFHLSYKDLDLDPAKFLSKNGYDLNLVVHSPELFAGDHLMDLSSA
                     NPDYRRRSEQELQRVIDVTRRLKPSFKKAHRPLIIINAGGFTLDAPLSVAARAPLYDR
                     IAESLSRVDAKGVEIIPQTMPPFPWHFGGQRYHNLFMDAADIVDFCKKHGVRVCLDVS
                     HSKLACTHAKASFQEFLEAVGPHTAHLHIVDAQGVDGEGLQIGEGEVDFRAMGATLDQ
                     TAPGVGFIPEIWQGHKNGGAGFWKALENLESFWRI"
BASE COUNT          243 a          365 c          373 g          246 t
ORIGIN      
        1 gtccccgacc cggatccctg ccacccgcca ctgatcggct aaattcgaac tcatggccgg
       61 actcttttat tgaagcggct gaacaattcc cctctggaaa agaacgtagt gataatcata
      121 ttcgccagaa cgattccaaa ttttcaagcg ccttccaaaa ccccgcgccg ccgtttttgt
      181 ggccctgcca aatttccggg atgaaaccga cgcccggcgc cgtttggtcc agggtcgcgc
      241 ccatggctcg gaaatcgact tcgccctccc cgatttgaag gccttcgccg tcgaccccct
      301 gggcgtccac gatgtgcagg tgcgcggtgt gggggcccac cgcctccagg aattcctgga
      361 aagacgcctt cgcgtgggtg caggccaact tggaatggga gacgtccagg cacacccgga
      421 cgccgtgttt tttacagaaa tcaacgatgt cggccgcgtc catgaagagg ttgtggtagc
      481 gctgcccgcc gaagtgccac gggaaaggcg gcatggtttg gggaatgatc tccacccctt
      541 tcgcgtccac ccggctcagg ctctcggcga tgcggtcgta caagggagcc cgggcggcga
      601 ccgaaagggg cgcgtccaag gtgaaccccc ccgcgttgat gatgatcaaa ggacgatgcg
      661 cttttttaaa cgagggcttc agccggcgcg ttacgtcgat cacccgctgg agttcctgct
      721 ccgagcgtcg acggtagtcg gggttggcgg aggagaggtc catcaaatga tccccggcga
      781 agagttcggg actgtgaacc accaaattca aatcgtatcc gttcttcgac agaaatttcg
      841 ccggatccag atccaaatcc ttgtagctga ggtgaaattc caacagatca aaattggatc
      901 gaccggccat ctcgttcaga tcgtggtaac ggatggggat gccgaagggg cggcgaaatc
      961 ggtagggacg gggttccgcc gccggggcct ccacgtcggt gggataaaag aaatcgccgg
     1021 gattgaaatc ccgccgggcg gcccgaccca ccaattcgtt ttttcggtag ggggccaacc
     1081 cgcggcccgg gcttcgaata tcgatcatgt cggccgtgat gggttcccct tggcggaggg
     1141 cccggttgat gacgaggctc ttggccagca cctcgcggtt catcatttcg ccctgggtca
     1201 ccttgcgggg ttccgatccg ccgaagg
//
LOCUS       DAOQLS010000737         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_100397_length_1226_cov_2.894108, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000737 DAOQLS010000000
VERSION     DAOQLS010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100397_length_1226_cov_2.894108"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK362_11110"
     CDS             complement(<1..244)
                     /locus_tag="PK362_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNC75415.1"
                     /translation="MPADGPSRPKAAGDPAHDVFRGDRGSLDVFFRPRSVAVVGATET
                     AGTVGRTLLWNLISSSFGGTVYPVNPKRPSVLGIKAY"
     gene            complement(234..725)
                     /locus_tag="PK362_11115"
     CDS             complement(234..725)
                     /locus_tag="PK362_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75416.1"
                     /translation="MNGAQLHLALIHFPVAGVFFALLLLAHAFWKKNGDVLNVALGFL
                     VLTGLAGLAAYWTGEGAEEVVEKAASVAGGWIHTHEEAAEAALIALEIAAGLALGGLL
                     FFRRSGGPRPAYVGAVFAATLLAAALLGRAAHHGGQIRHTEIRPPAAGTTASVESPDA
                     DAR"
     gene            complement(803..>1226)
                     /locus_tag="PK362_11120"
     CDS             complement(803..>1226)
                     /locus_tag="PK362_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75417.1"
                     /translation="PAGNRFFVRVNGVLHATPLFLTLVIVEFSDVVFALDSIPAIFSI
                     TQDAFIVYTSNVFAILGLRALFFLVAGLVQMFAYLKYGVALILAFVGVKMLIMHWVHI
                     STAASLSVVVGVLALSILLSVLVNRSRGPSSPAPAPPA"
BASE COUNT          270 a          355 c          439 g          162 t
ORIGIN      
        1 gataggcctt gatgcccagc acgctggggc gtttggggtt cacggggtaa accgtgccgc
       61 cgaaggagga ggaaatcagg ttccagagaa gggtgcgacc gacggtgccg gcggtttccg
      121 tggcgccgac cacggcgacc gagcggggac ggaaaaaaac gtcgagggag ccccggtccc
      181 cgcgaaaaac gtcgtgggcc gggtcgcccg cggccttcgg gcgggacggg ccgtcagcgg
      241 gcatcggcgt cggggctttc gacggaggcc gttgtcccgg cggcgggggg acggatttcc
      301 gtgtggcgaa tttgcccgcc gtggtgggcg gcgcgtccca ggagggccgc ggccagaagc
      361 gtcgcggcga acaccgcgcc gacgtaggcc ggccgggggc cgccggaccg gcggaaaaag
      421 agcaggccgc ccagggccag gcccgcggcg atttccaggg cgatgagcgc ggcttcggcg
      481 gcctcttcgt gggtgtggat ccagccgccc gccacggaag ccgctttttc aacgacctcc
      541 tcggccccct ccccggtcca ataggccgcc aaaccggcga ggcccgtcag gacgagaaac
      601 cccagggcga cgttcaacac gtcgccgttt ttcttccaaa aagcgtgggc caacagaagc
      661 agggcgaaaa agacgcccgc cacggggaaa tggatcaatg ccaaatgcaa ttgagcgccg
      721 ttcatggaac ctccaggaag atcatcgcca ttttattcga tttccgcacg ggcaaaaatg
      781 acgtgggtca gggttgaagg gattacgccg ggggggccgg cgcgggggag gaggggccgc
      841 gactccgatt gaccaggacc gatagcaaaa ttgacagggc gaggacgccg accaccaccg
      901 agagggaggc cgcggtggaa atgtgaaccc aatgcatgat caacatcttg acccccacga
      961 aggcgaggat caacgccacc ccgtatttta agtacgcgaa catctgcacc aagcccgcca
     1021 ccaggaaaaa gagggcgcgc aggcccaaaa tggcgaagac gttggaggtg tagacgatga
     1081 aggcgtcctg ggtgatggag aaaatggcgg ggatggaatc caaggcgaac accacgtcgc
     1141 tgaactcgac gatcaccagg gttaaaaaaa gcggggtggc gtgaagcacg ccgttgaccc
     1201 ggacgaaaaa acgattgccg gcgggg
//
LOCUS       DAOQLS010000738         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_101075_length_1222_cov_3.637532, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000738 DAOQLS010000000
VERSION     DAOQLS010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101075_length_1222_cov_3.637532"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1222
                     /locus_tag="PK362_11125"
     CDS             <1..>1222
                     /locus_tag="PK362_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC75418.1"
                     /translation="GIVLDTFRLSHLDGSPLSAAEEESVAFELQKFLDRPEIGENDFG
                     NGSLPRTPSGPMGSPGWLAGLTLRDIYHSLGLFFPPFHHMAEAVSPGVVGFPILPGND
                     KGVGASATPEAAATSKEPPHRPAEMTYDMTPEKRDHLAATVDYMRALVAGRRPPAVKR
                     LRKYTDDDYTFSHVYELLMSVSMNGTAEDRRTAIQLLDILILPRKTYSSLDYDLICFY
                     LATRAELLVQEGKIADALKSMKAHAELFDKYNASSDMVPATRLDMARFGAVKWARLAS
                     PLYDLQDFDNCRLAIESAGRYLNQAGVDEETRLTDPFNWVMSTRHLLEGMMLIRRGDA
                     VTASGKRSAQERRRLLEAALVHYQRSESFLPEEPFGLIQRAGALYRLDRNGEARRVLS
                     RALELGPDFGVAHAL"
BASE COUNT          209 a          417 c          377 g          219 t
ORIGIN      
        1 ggcatcgtcc tggacacctt ccggttgagc catctggacg gttcgccgct ttccgcggcg
       61 gaggaagagt ccgtcgcctt cgagctgcag aaattcttgg atcggccgga aatcggggaa
      121 aacgatttcg gcaacggctc cctgccccgg acgccctcgg gcccaatggg gtctccggga
      181 tggctggcgg gtctgacgct gcgggacatt tatcattccc tcggtctttt cttcccgccc
      241 ttccaccaca tggccgaggc ggtgagcccg ggcgtcgtgg ggttcccgat ccttcccggg
      301 aacgacaagg gcgtcggcgc ctccgccacg ccggaggccg cggcgacgag caaagagccg
      361 ccgcaccggc ccgccgaaat gacctacgac atgacgccgg aaaagcgcga ccacctggcg
      421 gccaccgtgg actatatgcg ggccctcgtc gccggccgtc ggccccccgc cgtgaagcgg
      481 ctgcgcaaat acaccgacga cgactacacc ttttcccacg tttacgagtt gctgatgtcc
      541 gtgtccatga acggcacggc ggaggaccgg cggacggcga tccaactgct ggatattttg
      601 atccttcccc ggaaaacgta ctcgtccctg gattacgatc ttatttgctt ctacctggcg
      661 acccgggccg agcttctggt ccaggagggc aaaatcgcgg acgccctcaa gtccatgaag
      721 gcccacgccg aattgttcga caaatacaac gcctcgtcgg acatggttcc cgcgacccgc
      781 ctcgacatgg cccggttcgg cgccgtgaaa tgggcccggc tggcctcgcc cctgtacgac
      841 cttcaggatt tcgacaactg ccgattggcc atcgagtcgg ccggacgcta tttaaaccag
      901 gcgggggtgg acgaggaaac ccgcctcacc gaccccttta actgggtgat gtccacccga
      961 cacctgttgg aaggcatgat gttgatccgt cgcggcgacg cggtcacggc gtccggcaag
     1021 cgatccgccc aggaacgccg ccggctgttg gaagcggcgc tcgtgcacta ccagcggtcc
     1081 gagtctttcc tgcccgagga acccttcggc ctcattcaac gggcgggcgc cctctaccgc
     1141 ttggaccgta acggggaagc ccgccgggtg ctgagccggg ccctggaatt gggccccgat
     1201 ttcggcgtgg cccacgctct cc
//
LOCUS       DAOQLS010000739         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_101607_length_1219_cov_3.114261, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000739 DAOQLS010000000
VERSION     DAOQLS010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101607_length_1219_cov_3.114261"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1075)
                     /locus_tag="PK362_11130"
     CDS             complement(86..1075)
                     /locus_tag="PK362_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75419.1"
                     /translation="MNRSRFLVVALLAIPALAVRAGFHMGLTGAAKSRAKHLEDKVNA
                     GPSVESRINTPGWADSPFISRDGQRLYFMYSRWDFGPFFLGGAPVLRGPDRPGHHNDD
                     VNPWDDSDIYVATKRGDGSWGAPVNMGFNSAGGDASGMELSSGTFFVWIKPTLPSGQP
                     DIYISTRDAFGNWSAPVGISTNINTVNLEDNPHLWPDGNSLWFTSDRGGGAGGKDIWF
                     PSKTAGVWNPPVNAGPTLNTAADEDQFWVSPVNFEVYFTRNNQIYRSSFTGLGYTAPT
                     LVNLGLAVAAEPSITDDGQDLYFATGDTTTAHIRIMHARSLGGGNWATPVPVD"
BASE COUNT          238 a          387 c          388 g          206 t
ORIGIN      
        1 gggccggttc gcggaaaagg cggtcgccgc gaatagccaa acaaccaccg caaaaaggga
       61 tcgacgaatc atgtcatctc cttgatcaat ccaccggcac cggggtggcc cagttgccgc
      121 cgcccaggga ccgggcgtgc atgatccgga tgtgggccgt ggtggtgtcg cccgtggcga
      181 aataaaggtc ctggccgtcg tcggtaatgg acggttcggc cgccacggcc aaacccaaat
      241 tcacgagcgt cggcgccgtg tacccgaggc ccgtgaagga cgaacggtaa atttgattgt
      301 tccgggtgaa atagacttcg aagttcacgg ggctgaccca aaattgatcc tcgtccgcgg
      361 ccgtgttcag ggtcgggccg gcgttgaccg gcgggttcca gacccccgcc gttttgctgg
      421 gaaaccaaat atccttcccg cccgctcccc cgccccgatc ggaagtgaac cacagggaat
      481 tgccgtccgg ccacaggtgc ggattgtctt cgagattgac ggtgttgatg ttggtgctga
      541 tgccgacggg ggcgctccag ttgccgaagg cgtcccgggt ggaaatataa atgtcgggct
      601 ggcccgacgg cagggtgggt ttgatccaca caaaaaaggt tccggaggac agctccatgc
      661 cgctggcgtc ccccccggcg ctgttgaatc ccatattgac gggggcgccc caggaaccgt
      721 cgccccgctt ggtggccacg tagatgtccg aatcgtccca ggggttcacg tcgtcgttgt
      781 ggtggcccgg gcggtccggt ccccgcagga ccggcgcccc gcccagaaag aaaggcccga
      841 aatcccaacg ggaatacatg aaatacaacc gctgtccgtc ccgggagatg aacggcgaat
      901 ccgcccagcc cggcgtgttg atccgggatt ccaccgacgg cccggcgttg accttgtcct
      961 ccagatgttt ggcccgggat ttcgccgccc cggtcaggcc catgtgaaaa cccgcccgga
     1021 cggccaacgc cggaatcgcc agaagggcga ccaccaaaaa gcgggatcgg ttcatcggga
     1081 aagggcccgg ccgaaataaa gattcacccc gacgttgacg acgaattcca gaccgccgtc
     1141 cgtgaggttc gtggcgttgt cttttaaccc gagccacccc gcgcccacgt ccaactgggc
     1201 cgccagccgc cgggcgaag
//
LOCUS       DAOQLS010000740         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_101763_length_1218_cov_3.257094, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000740 DAOQLS010000000
VERSION     DAOQLS010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101763_length_1218_cov_3.257094"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..478
                     /locus_tag="PK362_11135"
     CDS             128..478
                     /locus_tag="PK362_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNC75420.1"
                     /translation="MSDWSLYFNPRCGTCVKVKERLEARGVKPRVIEYLKQAPSVATL
                     KRLLEKMGAAPAAITRFKEPFWQEQNIDPATLKPEAWLRILSENPFLIQRPIVETETR
                     ALVARPPEEVDALF"
     gene            complement(548..934)
                     /locus_tag="PK362_11140"
     CDS             complement(548..934)
                     /locus_tag="PK362_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75421.1"
                     /translation="MEKTEAVQKKILVIENDLEEAARLAGACQGVGFDVRVESDRGQA
                     ALRAFEWRPDVIALDLEAPGLDGREVIRQLQSRPQTSRIPVLVLSGGGVPVELLTGEK
                     DRFIQKPRVLEGVVDSLRRLTSGPPN"
BASE COUNT          245 a          392 c          368 g          213 t
ORIGIN      
        1 tggacgtttt ggacgatatc gacgggccgg aagacgtttc ggcgctggcc caccgcctca
       61 aggaagcggg cgcccggtgc ccccacagcc gcgacgccct tctcaaactc aacaccggga
      121 gaatcccatg agcgactggt ccctctattt caacccacgg tgcggcacct gcgtcaaggt
      181 caaggaacgt ctggaggccc gcggggtgaa accccgggtg attgaatacc tcaagcaagc
      241 cccgtcggtg gccaccctca aacgcctgct ggaaaagatg ggggcggccc cggcggccat
      301 cacccgcttt aaagaaccgt tctggcagga acagaacatc gaccccgcca ccttgaaacc
      361 cgaggcttgg ctgaggattc tttcggaaaa tccgtttttg attcaacggc ccatcgtcga
      421 aacggagacc cgggccctgg tggcccgtcc gccggaagag gtcgacgctc tgttttaagg
      481 cccgctcggg tccaataaaa aaggcggtcc ccgtcgccgg ggaccgcctt tttgtttcgg
      541 gggaaattta gttggggggc ccggaggtca aacgccgaag gctgtcgacg actccctcca
      601 acacccgcgg cttctgaata aatcggtctt tctcccccgt cagcagttcc acggggacgc
      661 cgccgcccga caacaccagg accggaatgc ggctcgtttg agggcggctt tgaagttggc
      721 gaatgacttc gcgcccatcc aaccccgggg cttccaaatc cagcgcgatg acgtccggcc
      781 gccattcgaa ggcgcgcagg gccgcctgcc cccgatcgct ttccacccgg acatcgaacc
      841 ccaccccctg gcaggcgccc gccaaacggg cggcttcctc caggtcgttc tcgatgacga
      901 ggatcttctt ttggacggcc tcggtctttt ccatggtttc agattaccac ggccgcgaag
      961 gaaacgggat taagaaaatg taacaattat gtaacctttc gagccaataa agccgtcaaa
     1021 acgtctttat tgggcgggag gttggtttgg aggggaaccg cgttgaaaac cgcggcgggc
     1081 ggccaattcc tgggcgcgca gacgagccag ggcgcgccaa cgcagggcga gttcctgacc
     1141 tcgctgctcg acatcacgcg cggcagccag tcggcgcagt gggggaccct ggcgacggcc
     1201 ggcgtcgcca acgaagac
//
LOCUS       DAOQLS010000741         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_101782_length_1218_cov_2.927773, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000741 DAOQLS010000000
VERSION     DAOQLS010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101782_length_1218_cov_2.927773"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1218
                     /locus_tag="PK362_11145"
     CDS             28..>1218
                     /locus_tag="PK362_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNC75422.1"
                     /translation="MASPRLEKWRQNLDIDGISLVFATAYLNNPASMFGHTFLRLHRR
                     GTGADPLLDYTANFAAVTGETSGLVFAVKGLLGGYPGKFSTHPYYMKIQQYNNMENRD
                     LWEYDLALSTAQVRRLLDHLWEMGPTYFHYFFLTENCSYQLLPLLEVADPALSLSDGF
                     AWKAIPVDTLRRVLAAPGFVAARRGRPAQLKFIFARRDLLSPTERRWVERASRPGPLP
                     SAEGFPLERRALLWESAYDYYRLRHKFRRYGAPAQEAMERQLLMERSRVAAPGPDVSS
                     AALAALEPPEVGHPTGRVGLATGRGAGKGFEELELRTSLHDLTDPSPGFPRGSRLSMF
                     TAKFRYLNETRGVALERGTLVDIFSLTPWDPWARSPSWRFFMGWDRARDRFKKPSGGL
                     HFTLN"
BASE COUNT          192 a          386 c          392 g          248 t
ORIGIN      
        1 gggttcgacg gaacccgtct gcctgaattg gcgtcgcccc gccttgagaa atggcggcaa
       61 aatctggaca tcgacgggat ctccctggtc ttcgccacgg cctatttgaa caacccggcc
      121 tccatgttcg ggcacacttt tttgcgcctt catcggcggg ggaccggggc cgacccgctg
      181 ttggactaca cggcgaattt cgcggcggtg acgggcgaaa ccagcggctt ggtttttgcg
      241 gtcaaagggc ttttgggcgg ctacccggga aaattttcca cccaccccta ctacatgaaa
      301 attcagcagt acaacaacat ggagaaccgg gacctctggg aatacgattt ggcgctgtcc
      361 acggcccagg ttcgccggct cttggaccac ctgtgggaga tgggacccac gtacttccac
      421 tatttctttc tgacggagaa ttgctcgtac caattgctgc ccttgttgga ggtggcggac
      481 ccggccttgt ccctaagcga cgggttcgcc tggaaagcga ttcccgtgga caccctgcgg
      541 cgggtgttgg cggcgccggg gttcgtggcc gcgcggcggg gccggcccgc ccagttgaaa
      601 tttattttcg cgcggcgcga cctcctgtct ccgacggaac ggcgctgggt ggagcgggcc
      661 tcccggcccg ggccgctccc gagcgccgag ggttttccct tggagcggcg ggccctcctg
      721 tgggaatcgg cctacgatta ctaccgcctg cgccataaat tccgacggta cggagccccg
      781 gcccaggagg ccatggagcg tcaattgctg atggagcgga gccgcgtggc cgcccccggg
      841 ccggacgttt cctcggccgc cctggccgct ttggagcctc ccgaggtcgg ccacccgacg
      901 ggccgcgtgg gactggcaac gggccgtggc gccgggaaag gattcgagga attggagttg
      961 cgcacgtccc tccacgattt gaccgatccc tcccccgggt ttccccgggg gagtcgtctc
     1021 agcatgttca ccgccaaatt tcggtatctc aatgaaaccc gaggggtcgc ccttgagcgg
     1081 gggactctcg tggatatttt ctccctgacg ccttgggacc cctgggcccg ttcgccctcc
     1141 tggcggtttt tcatgggttg ggaccgggcc cgggaccgtt ttaaaaaacc cagcggggga
     1201 ttgcatttca ccctgaac
//
LOCUS       DAOQLS010000742         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_101803_length_1218_cov_2.570937, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000742 DAOQLS010000000
VERSION     DAOQLS010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101803_length_1218_cov_2.570937"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1218
                     /locus_tag="PK362_11150"
     CDS             <1..>1218
                     /locus_tag="PK362_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNC75423.1"
                     /translation="EGLWPRDVDYADPRYPEARRAEEDWAKPLIESGPVEALQPLLKA
                     LALLTTDQEERLLGELSAAVAKNASLADRPIVDGLFLMTGRIPKEWAKLHGIAYQRVY
                     HISAEGRKVWAGGLGPVQVFHSDAERQITRDSAIDIVDIEPDYTHRRTAEGMVPAERV
                     RPSTPLKPSHEPPFDLEVMFHGHPVKIRVRPMRGPEGNPVYLFRDNPDGDAHFTKQLY
                     DYRPSGDNPASWEEFTAFFGVATLAIIGRLEENRQKTLGAQWRPPVVHGNDAQTAMAI
                     VLLAEIAKGGNPRAAELVKSVPFLGKLAPLAARSFKTHTYGNRQGYTWGRPEDHYGVA
                     SYLSGIPEWDHDRLNFYFARRGPEAHGYPDVTAAAIAATPGHAQGVARKHGWDVMRKY
                     DDPQKVVGITNGA"
BASE COUNT          244 a          401 c          369 g          204 t
ORIGIN      
        1 ggagggactg tggccccggg acgtcgatta cgcggatccc cgataccccg aagctcgccg
       61 cgccgaggag gattgggcca aaccactcat cgaatcgggc cccgtggaag ccttgcaacc
      121 cctgttgaaa gccctcgctc tcttaaccac cgatcaggaa gagcgcctcc tgggcgaatt
      181 gtccgccgcg gtcgccaaga acgcctcttt ggccgaccgg ccaatcgtgg acggactctt
      241 tctgatgacg ggacggatcc ccaaggagtg ggccaaattg cacggcatcg cttatcagcg
      301 cgtctaccat atttccgccg agggccgaaa agtttgggcg ggcggcctcg gacccgtcca
      361 agtgtttcac agcgacgcgg aacggcagat cacccgggac tccgccatcg acatcgtgga
      421 catcgaaccg gattacaccc accgccggac ggccgaaggc atggtccccg ccgagcgggt
      481 tcgaccgtcg acgccgctca agccctccca cgaacccccc ttcgatttgg aagtgatgtt
      541 tcacggtcac cccgtcaaaa tccgggttcg gcccatgcgc gggcccgaag gcaatccggt
      601 ctacctcttc cgggacaacc ccgacggcga cgcccatttc accaaacagc tttacgatta
      661 ccggcccagc ggcgacaacc cggcttcctg ggaggaattc accgcgtttt tcggcgttgc
      721 gactttggcg atcatcggtc ggttggaaga aaatcgccaa aagaccctgg gcgctcaatg
      781 gcgcccgccg gtggtgcacg gcaacgacgc ccaaaccgcc atggccatcg ttctgttggc
      841 cgagatcgcg aaagggggca atccccgggc cgcggaactg gtgaaatccg tgcctttcct
      901 ggggaaattg gccccgttgg cggcccggtc ctttaaaacg cacacctacg ggaatcggca
      961 gggttacacc tggggacggc cggaggacca ttacggcgtg gcttcctacc tgagcggaat
     1021 tcccgagtgg gatcatgacc gattgaattt ttatttcgcg cgtcggggac cggaagccca
     1081 cgggtatccg gacgtcacgg cggcggccat cgccgccacg cccggtcacg cccaaggggt
     1141 ggcccgcaaa cacgggtggg acgtcatgcg gaaatacgac gatccccaaa aggtggtcgg
     1201 catcaccaac ggggccaa
//
LOCUS       DAOQLS010000743         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_101884_length_1217_cov_5.015491, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000743 DAOQLS010000000
VERSION     DAOQLS010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101884_length_1217_cov_5.015491"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PK362_11155"
     CDS             <1..224
                     /locus_tag="PK362_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC75424.1"
                     /translation="LDAQSEKLVQEAVERLMKNRTVLVIAHRLATVKNASRIVVLEHG
                     QIAEVGSHAELLAKDGIYRRLYELQLLER"
     gene            221..943
                     /locus_tag="PK362_11160"
     CDS             221..943
                     /locus_tag="PK362_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNC75425.1"
                     /translation="MTAAPLIFSAVSVGATLGGAHLILRRPAWAEAHLWRILAFGSGV
                     LLAMTFLHLLPEAWGMNPRWASAAVLAALGGLFVLEEFTVVHACGEVAEHCVRHHVGY
                     GALAALFLHSLADGLAIAFAFQSSERLGTAVALAVVVHKFSDGMTLSSLFLGAGHAPG
                     RAGRLSTVLSLATPLGVLTGGFLGPRAAGPGLAVLLGLAGGGFLYVSMADVLPRIHRN
                     RDPWCWTFLVVGMIAGATLPHP"
     gene            940..1140
                     /gene="thiS"
                     /locus_tag="PK362_11165"
     CDS             940..1140
                     /gene="thiS"
                     /locus_tag="PK362_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNC75426.1"
                     /translation="MKLRLNGEDREIPTVATLADLLAHLKLAPGRLACEVNGAVVRRA
                     DYAAAALREGDQIEIVQMIGGG"
BASE COUNT          177 a          403 c          402 g          235 t
ORIGIN      
        1 ccctggacgc ccagtcggag aaactggtcc aggaggccgt ggagcggctg atgaaaaacc
       61 gcacggttct ggtgatcgcc caccgcctgg ccaccgtcaa aaacgccagc cgcatcgtgg
      121 tcttggaaca cggccagatc gccgaggtcg gaagccacgc ggaactcttg gccaaggacg
      181 gcatttaccg gcgcctctac gaattgcagc tcctcgaacg atgacggccg cgcccctgat
      241 tttttccgcc gtttccgtcg gggcgaccct ggggggcgcc catttgattc tgcggcgccc
      301 ggcctgggcc gaagcccacc tctggcgcat tctggccttt ggaagcggcg tcctgttggc
      361 catgaccttc ctccaccttc tgcccgaggc ctgggggatg aatccccggt gggccagcgc
      421 ggccgtcctg gccgccctgg gcggactgtt tgtgttggag gaattcacgg tggtccacgc
      481 ctgcggggaa gtggcggagc attgcgtgcg ccatcacgtg ggctacgggg ccttggccgc
      541 cctgtttctt cacagcctgg ccgacggttt ggccatcgcc ttcgcctttc aatcctcgga
      601 acgcttgggc acggcggtgg ccctcgccgt ggtcgtccat aaattttccg acggaatgac
      661 cctgtcgtcc ttgtttttgg gcgcgggcca cgcgccgggc cgggccggcc gactttccac
      721 cgtcttgtcc ctggccaccc ctctgggggt gttgaccggc ggcttcctgg ggccccgggc
      781 cgccggaccc gggttggcgg tgctgttggg attggccggc ggcggattcc tttacgtcag
      841 catggccgac gtgctgcccc gcatccaccg taaccgcgac ccgtggtgct ggacgtttct
      901 cgtggtgggc atgatcgccg gcgccacgct cccccacccg tgaaattgcg cttgaacggg
      961 gaagatcggg aaattccgac ggtggccacc ttggccgacc tgctggctca tttgaaattg
     1021 gcgcccgggc gtctggcctg cgaagtgaac ggcgccgtgg tccggcgggc cgactacgcc
     1081 gccgcggccc tccgcgaggg cgatcaaatc gaaatcgtcc aaatgatcgg cggcggctga
     1141 ccccgcgtcg tccgaaaagc tagtataagg agaattccat gaacgaccga ccgctggtga
     1201 ttgccggacg aaccctg
//
LOCUS       DAOQLS010000744         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102012_length_1217_cov_2.040448, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000744 DAOQLS010000000
VERSION     DAOQLS010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102012_length_1217_cov_2.040448"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..>1217
                     /locus_tag="PK362_11170"
     CDS             8..>1217
                     /locus_tag="PK362_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNC75427.1"
                     /translation="MTPPRPAGDSLKKRYGYKLVSNGIGMVALLGTQAIVPRALGPAD
                     YGLFGYLTNFFTQTIELFDAGVTLGFYTKLAQRPGDHGLVRFNWGVLAAIGLLVVGAT
                     FLLHQLGLGRSVWLSTPMRFVYLAIAWCLLSKGAAVVGLMADAHGLTTSLELGRNLQR
                     FVGLSGLLALAWWGGLRLSTYFLYGQAMSVALVVAGGIVLRRHGFPLWPRRPLTSVEI
                     KGYAAELGEYATPLLWLTAVGMVVSLLDRWLLQAYGGAVQQGFYGFSMQMGSLCFVFA
                     ASLTPLLMRKMSKASAENDRERQRHLFLRLIPPLFSVSAFFGLFLAFQADRVIDLFAG
                     GRYHDARLAVAVMCLYPLHQTYGQLSGSVFYSTGQTRLYRNIGVTMTLLGLPLTYFFL
                     APASVGGQNGG"
BASE COUNT          159 a          389 c          397 g          272 t
ORIGIN      
        1 agcgcccgtg accccgccgc gcccggcggg ggattcgctc aagaaacgct acgggtacaa
       61 actggtctcg aacggcatcg gcatggtcgc ccttttgggc acccaggcca tcgtgccgcg
      121 ggccctcgga ccggccgact acgggttgtt cggctacctg acgaatttct tcacccaaac
      181 catcgagttg ttcgacgccg gcgtcaccct cggcttttac acgaaactgg cccagcggcc
      241 cggcgaccac gggctggtcc gtttcaactg gggcgtgctg gcggccatcg ggctcctggt
      301 ggtgggggcg acgttcctcc tccatcaatt gggtctgggg cggtcggtgt ggctctcgac
      361 cccgatgcgt ttcgtctact tggccatcgc ctggtgcctc ctctcgaaag gggcggccgt
      421 ggtcgggttg atggccgacg cccacggatt gacgacgtcc ctggagttgg gccggaatct
      481 tcagcggttc gtcggtctgt cgggcctgtt ggcgttggcg tggtggggag gtctccgact
      541 gtccacctat ttcctctacg gccaggccat gtccgtggcc ttggtcgtcg cgggcggcat
      601 cgtccttcgc cgccacggtt ttcccctgtg gccgcgccgc ccgctgacgt ccgtcgagat
      661 caaaggatac gccgccgaat tgggggaata cgccacgccg ttgttgtggc tcaccgccgt
      721 gggcatggtc gtctcccttc tggatcgatg gctcctgcag gcgtacgggg gggcggttca
      781 acaggggttt tatggatttt cgatgcaaat gggctcgctc tgtttcgtgt tcgcggcctc
      841 cttgacgccc ctgttgatgc gcaagatgtc caaagcgagc gccgagaacg accgggagcg
      901 ccagcgccat ctttttctcc ggctgatccc gcccctgttt tccgtttccg cgttcttcgg
      961 ccttttcctg gcgttccaag cggaccgggt gatcgacctc ttcgcggggg gccggtacca
     1021 cgacgcccgg ttggccgtgg cggtcatgtg cctctacccg ttgcaccaga cctacggcca
     1081 attgagtgga agcgtttttt actccaccgg ccaaacgcgc ttgtaccgga acatcggggt
     1141 gacgatgacg ctgctggggc tgcccctgac gtattttttc ctggcgccgg cgtccgtcgg
     1201 cggtcaaaac ggcgggg
//
LOCUS       DAOQLS010000745         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102038_length_1217_cov_1.401893, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000745 DAOQLS010000000
VERSION     DAOQLS010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102038_length_1217_cov_1.401893"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..681)
                     /locus_tag="PK362_11175"
     CDS             complement(28..681)
                     /locus_tag="PK362_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC75428.1"
                     /translation="MPYIVLVLFFLIIGWFFTSALFLEGQANLDNFFGMMPLLLGIFL
                     PAFTMRLFAEEFKTGTIETLATLPLDDSEIVLGKYGAVLSVWLAMMGLSLFYAALLAV
                     LGPPDLGQMAGGFAGAILLGAFYGALGLFASSLTRSQVVAFLLGFLFCFAFFIVGRAA
                     DYTPGVPGQLLAFLGVDRHFEGFLKGVADSRDVLYFVSGTVLSLAGTLASFNSRRWR"
     gene            795..>1217
                     /locus_tag="PK362_11180"
     CDS             795..>1217
                     /locus_tag="PK362_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC75429.1"
                     /translation="MQVGACMIGTFTDLHEAIDGNRCLLYHVIGGGTGDWDVPIGGMG
                     VVSGGMERAARAAGAKLVTGAEVTAIDPDGAVEYVRGEQQHRVTADLVLAGVAPGVLA
                     RLLGETPTVRAEGAQVKVNLLLRRLPGLRQRGVRPEAAF"
BASE COUNT          270 a          378 c          398 g          171 t
ORIGIN      
        1 atcggaattt gcggccgcgg ggggcgatca tcgccatctc cggctgttga agctggccaa
       61 ggttcccgcg agggagagga cggttccgga cacaaaataa agcacgtcac gggaatccgc
      121 cacgcctttc aaaaaccctt caaagtgacg gtccacgcca agaaacgcca acagttggcc
      181 cggcacgccg ggggtgtaat cggccgcccg accgacgata aagaacgcga aacagaacaa
      241 aaaccccaac agaaaagcca ccacttgact gcgcgtcagc gaggaggcaa agaggcccag
      301 cgccccatag aaagccccca acagaatcgc gcccgcaaac ccgccggcca tttgccccag
      361 gtccggcggg ccgaggacag ccaagagggc cgcgtaaaac agcgacagcc ccatcatggc
      421 caaccagacc gataggaccg ccccgtattt ccccaggacg atttcgctgt cgtccagggg
      481 cagcgtcgcc agggtttcga tggtaccggt cttaaactct tcggcaaaaa gccgcatggt
      541 gaaggcgggc aaaaatattc ccagcagaag cggcatcatc ccgaaaaaat tgtccaagtt
      601 cgcttgcccc tccaaaaaga gcgcgctggt aaaaaaccag ccgatgatga ggaagaacag
      661 gaccaaaacg atgtaaggca tgggggaatt gaaggagtcg gcccattctt ttttggttaa
      721 aagccaagcg cgggaaactt tcatttcgac tccgtgaact gtcggaaaat gtcatccagg
      781 gcggcggcgg gacggtgcag gtcggcgcct gcatgatcgg cacgttcacc gacctgcacg
      841 aggccatcga cggcaaccgc tgcctgctct accacgtgat cggcggcggc accggcgact
      901 gggacgtgcc gatcggcggc atgggcgtgg tgtccggggg catggagcgg gccgcgcggg
      961 ccgccggtgc gaagttggtc acgggggccg aggtgacggc gatcgatccc gacggcgccg
     1021 tcgagtacgt gcgcggcgag cagcagcacc gggtgaccgc cgacctggtg ctggccgggg
     1081 tcgctcccgg cgtgctcgcc cgactgctcg gcgaaacccc gacggtgcgg gccgagggcg
     1141 cccaggtgaa ggtgaacctc ttgctgcgcc ggctgcccgg gctgcggcag cggggcgtcc
     1201 ggcccgaggc cgcgttc
//
LOCUS       DAOQLS010000746         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102122_length_1216_cov_3.050818, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000746 DAOQLS010000000
VERSION     DAOQLS010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102122_length_1216_cov_3.050818"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1216
                     /locus_tag="PK362_11185"
     CDS             <1..>1216
                     /locus_tag="PK362_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNC75430.1"
                     /translation="ADATLWVATRDQAVIGHQPGGLDILLDKDTVRAGGPLSVLVLAP
                     ASGRHVLFGVEAAGLESLKLLSMDGAVKMVEVPVGEKHIPNFFLSATMVSDYEISEVS
                     KEVVVPPEDKFLTVEVKPDNTDHKPGEAGKWTVTTKDRAGKPVAADVSFAVSDEAVNA
                     IQSDLAGDPRAHFYGQKRYSRVRSASTFNWRNYPSDVNQTENSLEEKEMVPSGSMRER
                     RSAAMPLGAAKMKKGMARDEMDDGMVMKSAPAPAMEAQSELSQDKDSGGGGEPAVQVR
                     QNFSATAYWQPDLQTGADGEATVSMKFPDTLTSWQGHARAFTTATQVGEAKASVRTRA
                     PLMVRLQAPRFFVVGDEVVLSALVNNVSDRDMSVQVSLASGDGLSGPSGAERFTVPAG
                     GEARLDRRFKVVK"
BASE COUNT          223 a          384 c          427 g          182 t
ORIGIN      
        1 ggcggacgcc accctctggg tggccacccg ggaccaggcc gtcatcggcc accagcccgg
       61 cgggttggac attttgctgg acaaagacac cgtgcgggcc ggcggacccc tgagcgtgct
      121 cgtgctggcc cccgcctcgg gccgtcacgt cctcttcggc gtggaggccg cggggttgga
      181 aagcctgaag ctcctctcca tggacggggc ggtcaagatg gtggaggtgc cggtggggga
      241 aaagcacatt cccaatttct tcctctcggc caccatggtg tcggactacg aaatttcgga
      301 agtgagcaag gaagtcgtcg ttccgccgga ggacaagttc ctcacggtcg aagtcaaacc
      361 cgacaacacg gaccacaaac ccggcgaagc cggcaaatgg accgtgacga ccaaagaccg
      421 ggcgggcaaa cccgtggcgg cggacgtgtc cttcgccgtt tcggacgagg cggtgaacgc
      481 cattcaatcg gacctggcgg gggacccccg ggcccacttc tacgggcaaa agcgatattc
      541 ccgggtgcgg tcggcctcca cctttaactg gcggaactac ccctcggacg tgaaccagac
      601 ggaaaactcc ctggaggaaa aagaaatggt cccctccggc tccatgcggg aacggcggtc
      661 ggccgccatg cccctgggcg ccgcgaaaat gaaaaaaggc atggcccggg acgagatgga
      721 cgacggaatg gtcatgaagt cggcccccgc gccggccatg gaggcccaaa gcgaattgtc
      781 ccaggacaag gattccggcg gcgggggaga gcccgcggtg caagtccggc aaaatttctc
      841 ggccacggcc tattggcagc cggaccttca aaccggcgcc gacggcgagg ccacggtgtc
      901 catgaagttc ccggacacgc tgacttcctg gcaggggcac gcccgggctt tcaccacggc
      961 gacccaggtg ggcgaggcca aggcctcggt gcggacgcgg gcgccgctca tggtgcggct
     1021 ccaggcgccg cggtttttcg tggtggggga cgaggtcgtt ctctcggccc tggtgaacaa
     1081 cgtgtcggac cgggacatga gcgtgcaggt gtccctggcg tccggggacg gcctctccgg
     1141 cccttcgggg gcggagcgct tcactgtgcc cgccgggggc gaagcgcggc tggaccggcg
     1201 gttcaaggtg gtcaag
//
LOCUS       DAOQLS010000747         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102250_length_1215_cov_3.827586, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000747 DAOQLS010000000
VERSION     DAOQLS010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102250_length_1215_cov_3.827586"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1215
                     /locus_tag="PK362_11190"
     CDS             39..>1215
                     /locus_tag="PK362_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75431.1"
                     /translation="MQSYVDDALGRFLSAFSSVAPDRPVLSVLSLAGSAAADRPVRWA
                     GTGRRGWAYWSEPGVGLRESEVRGVWCLRHPSLPNGRLLAQPGQLVDVGPSLTALMGL
                     GPPGGGDGRALDFRRESPTEDTVVHIAFRGREAKGLLIDGHYKYIRHRGPTPRRWRPR
                     RFWIDYPREEVFDLWNDPAEQQNLAIRRRTLLARLRQSMDEIDPDPTDIRVAFRGADG
                     ARAQGAATCSTYGLRVSEDSTLPLVSRGRTEFSFTVPASSGAVIFETDPPGASYMMYL
                     TVNGRYMAPSRLRVSRLNLPLFESAGDGWHDRIKFPWMDGWAPLPATATETTAYLGRR
                     ERDPKVWRPALDRLAESPVWTPFPTTWVVGSGRLPAMTAPPPVPLAAPVPAVSTFERP
                     "
BASE COUNT          154 a          425 c          418 g          218 t
ORIGIN      
        1 ggtcccaccg acgatgggct cgttggcggg aagccgccgt gcaatcctac gtcgacgacg
       61 cgctggggcg gtttctgtcg gctttttcct ccgtcgcccc cgatcgtccg gtcctgagcg
      121 tcctgtccct ggcgggcagc gcggccgccg accggccggt gcgttgggcg gggaccggtc
      181 gaaggggctg ggcctattgg tccgaaccgg gcgtgggcct gcgggaaagc gaagtgcgcg
      241 gggtctggtg tctgcggcac ccgtccctgc ccaacgggcg cctgttggcc caaccgggcc
      301 agttggtgga cgtggggccc tccctgaccg ctctgatggg gctgggcccc ccggggggcg
      361 gcgacgggcg ggcgctcgac ttccgccggg aatcgccgac ggaggacacc gtcgttcaca
      421 tcgcttttcg cggccgggag gccaagggcc ttctcatcga cgggcattac aaatacatcc
      481 gtcaccgggg accgacgccg cggcggtggc gcccccggcg cttttggatc gattaccccc
      541 gggaggaagt ttttgacctt tggaacgatc cggcggaaca gcagaacctg gcgatccgac
      601 ggcgcacgtt gttggcccgc ctccgccagt ccatggacga gatcgatccc gatcccacgg
      661 acattcgggt ggcctttcgg ggggcggacg gggcgcgggc ccaaggggcc gccacctgtt
      721 ccacctacgg tcttcgggtg agcgaggact cgaccctgcc cttggtttcc cggggccgga
      781 ccgaattttc ctttaccgtt cccgcgtcct ccggggcggt gatctttgaa accgatcccc
      841 cgggcgcctc gtacatgatg tacctcaccg tcaacggccg atacatggcg ccgtcccgtc
      901 ttcgggtgtc ccgtttgaat ttgcccctct tcgagtccgc gggcgacggg tggcacgacc
      961 gcattaaatt cccctggatg gacgggtggg ctcccctgcc cgccacggcc acggaaacca
     1021 ccgcctattt gggccggcgc gaacgcgacc ccaaagtttg gcggcccgcc ctggatcgac
     1081 tggcggagtc ccccgtgtgg acgcctttcc cgacgacgtg ggtggtgggg agcggccgtt
     1141 tgcccgcgat gaccgcgccc ccgcccgttc cgctcgccgc gcccgttccc gccgtttcga
     1201 ccttcgagcg accgc
//
LOCUS       DAOQLS010000748         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102266_length_1215_cov_3.325862, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000748 DAOQLS010000000
VERSION     DAOQLS010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102266_length_1215_cov_3.325862"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK362_11195"
     CDS             <1..364
                     /locus_tag="PK362_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNC75432.1"
                     /translation="TGLPADQSRSRAKTPRLQILNLKEPTGQRPVLKVAPLVDWTEER
                     VRAYLRENNVPVHALLEKTLPGGYYYESLGCVLCTTPIGPNESRRAGRWRWFNTSDDK
                     KECGLHLPPHDPTSPDVP"
     gene            361..1170
                     /locus_tag="PK362_11200"
     CDS             361..1170
                     /locus_tag="PK362_11200"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNC75433.1"
                     /translation="MTDPSVFLGPARDLAVAAGRKVLALLKDPLVRSRKADHSLVTNA
                     DHAANDLLREGLRSRFPEHALLSEETGLEGPADAPLRWLVDPLDGTRAYARGEPGFSV
                     MIGLLHAGRPVLGVVYDPLADRLYDAARGHGAFLTEGGRRSPLRVTERWAWPEMPVIT
                     STGFPAVLRERLATDPGLKFLDPINSVGIKVGYLVRGAADLYLNHHPVHYWDTAGPLA
                     ILEEAGGRMTHWDGSPLTYNGDDRYAHAQPTVASNGPRHADVVTLVRGALE"
BASE COUNT          195 a          428 c          388 g          204 t
ORIGIN      
        1 caccggcctt ccggccgacc aatcccggtc ccgggccaag accccccgcc tgcaaatcct
       61 caatctcaag gaaccgacgg gccaacggcc cgttctcaaa gtcgcgccct tggtggattg
      121 gaccgaggag cgggtgcggg cctaccttcg ggaaaacaac gtcccggtcc acgccctgct
      181 cgagaaaacg cttcccggcg gctattatta cgaatccctg gggtgcgtgc tttgcacgac
      241 gcccatcggg cccaacgaat cccgccgcgc aggccggtgg cgctggttca acacctccga
      301 cgacaagaag gaatgcgggc tgcacctgcc gccccacgac ccgacgtccc ccgacgttcc
      361 gtgacggacc cttccgtttt tctggggccc gcccgggacc tggcggtggc cgccgggcga
      421 aaagtgttgg cgcttttgaa ggaccccctc gtgcgctccc gaaaagccga tcattccctg
      481 gtcaccaacg ccgaccacgc cgccaacgac ctgctgcggg agggccttcg gtcccgcttt
      541 cccgaacacg ccctgttgag cgaggaaacc ggtctcgagg gcccggccga cgccccgctc
      601 cggtggttgg tggatccctt ggacggcacc cgggcctacg cccggggcga gcccggcttc
      661 agcgtcatga tcggccttct gcacgcgggg cgaccggtgc tcggcgtggt gtacgacccc
      721 ttggccgacc ggttgtacga cgcggcccgg gggcacggcg ccttcctcac ggaaggcggc
      781 cggcgatccc ccctgcgggt caccgaacgc tgggcgtggc cggaaatgcc ggtcattacc
      841 tcgacgggat ttcccgcggt tctccgggaa cgcctggcga ccgatccggg attgaaattt
      901 ttggacccca tcaacagcgt cgggattaaa gtgggttatc tggtccgggg ggcggcggat
      961 ctctacttga accatcatcc ggtccattat tgggacaccg cgggtcccct cgccatcctg
     1021 gaggaagccg gcggccggat gacccattgg gacggttctc cgttaacgta taatggggac
     1081 gaccggtacg cccacgccca gcccacggtg gcttccaacg gcccgcggca cgcggacgtg
     1141 gtgacgttgg ttcgcggggc gctcgaatga cgtttcgccg attcgttccc cgcgaggaac
     1201 gcgcgaggcc ccaat
//
LOCUS       DAOQLS010000749         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102268_length_1215_cov_3.238793, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000749 DAOQLS010000000
VERSION     DAOQLS010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102268_length_1215_cov_3.238793"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1215)
                     /locus_tag="PK362_11205"
     CDS             complement(<1..>1215)
                     /locus_tag="PK362_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75434.1"
                     /translation="QVSTHIEKQADGSLLARTGQGNNLYDRPYQATFRMDPTGRTLAL
                     QSDRNGGSVVTAMSLAQFWQSNGLAVRDRQTTASSDSLVLRRPDGSFQQTTYEKQDNG
                     DVRAVTVLVDLEGHVTTLDVTFDSDGKVLRQNVADARWAGVQTGAGSETKTDELDSEG
                     NKVQPKEPPPSPQAVAFGQLVGLKPVWPKSTAGSQLEISGKVDGDVQRSVVLRRETDG
                     TVTAAVTDTAPDGTTRRLAYRLDDDANVLATVTDFGTPQAADLGSGFWNAVNVPATDN
                     RSNTGSNNWITRNFSLQPASSRPPARGSYQNTVKNLPGGVKQNSQLVRGSDGQLRLLS
                     TVTDVAGHTRSIFVDVPGALAASTKTVKQPTAETRSVTALRLLDNSTLTVESTRKANG
                     TVQTELSWKTAAG"
BASE COUNT          203 a          357 c          403 g          252 t
ORIGIN      
        1 gcccgcggcg gttttccaag acaattcggt ttggaccgtg ccgttggctt tgcgggtgga
       61 ctccaccgtc agcgtggaat tgtccaacaa gcgcagcgcc gtgaccgaac gggtttcggc
      121 ggtgggttgt ttgaccgttt tggtcgaagc ggccagcgct ccgggaacat cgacaaaaat
      181 ggaccgtgtg tgtcccgcga cgtccgtcac ggtcgaaaga aggcgcaatt gcccgtccga
      241 gccccgcacc agctgcgagt tttgcttgac gcccccgggc aggtttttga cggtgttctg
      301 ataagagccc cgggccgggg ggcgcgagga ggccggttga agactgaagt tgcgcgtaat
      361 ccagttgttg gatcccgtgt tcgatcggtt gtccgtggcc gggacgttga cggcgttcca
      421 gaatcccgaa cccagatccg cggcctgggg ggtcccaaaa tccgtaacgg tcgccaagac
      481 gttggcgtcg tcgtccaatc ggtaggccaa ccgccgcgtg gttccgtccg gcgcggtgtc
      541 ggtcacggcg gcggtcacgg tgccgtcggt ttcgcggcga aggacgacgg accgttggac
      601 gtcgccgtcc actttaccgg aaatctccaa ttgagacccg gcggtcgatt tcggccaaac
      661 gggcttaaga ccgaccaact ggccaaacgc gacggcttgg ggagacggcg ggggttcctt
      721 gggctgcact ttgttccctt cggaatccaa ttcgtccgtc ttcgtttcgc ttccggcgcc
      781 ggtttgcacg cccgcccaac gggcatcggc cacgttttgg cgaaggacct ttccgtcgct
      841 gtcgaaggtc acgtccaggg tggtgacgtg tccttccagg tcgacaagga ccgtaaccgc
      901 tcgaacatcg ccgttgtctt gtttttcata cgtggtctgc tggaaagatc catcgggacg
      961 gcgaaggacc agcgagtccg aggaagcggt ggtttgacgg tcccggacgg ccaacccgtt
     1021 gctctgccaa aattgcgcca gcgacatggc ggtcacgacg gacccgccgt tacggtcgga
     1081 ctggagcgca agggtgcgcc cggtggggtc catacggaac gtcgcttggt acggccgatc
     1141 gtacaagttg ttcccctgac cggttcgggc caacaacgag ccgtcggcct gtttttcaat
     1201 gtgggtcgac acttg
//
LOCUS       DAOQLS010000750         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102453_length_1214_cov_3.171700, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000750 DAOQLS010000000
VERSION     DAOQLS010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102453_length_1214_cov_3.171700"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..932)
                     /locus_tag="PK362_11210"
     CDS             complement(87..932)
                     /locus_tag="PK362_11210"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HNC75435.1"
                     /translation="MRRPLYSAVFFLGFLLIPASSRPGPYPWPHAAEGETIHSRFQAP
                     RGTARLSPPENSFGGWLQRLPLKSGRPPVRLYDGTLKNRQDVHAAVVDMPIGNRDLEQ
                     CADAVIRLRAEYLRAAGREADVCFRFTNGDPCPWPRWAGGDRPSVLGSRVAWFHRAST
                     DTSRASFEDYLFTLFRWAGTLSLSRELAAVPAGGPPEIGDVYIQGGSPGHAVIVADAA
                     RARDGSTWVLLLQSYMPAQEIHVLKNADTGEPWFPAPADGSMNSPQWAFPARSLKRFS
                     EHGCP"
     gene            complement(971..>1214)
                     /locus_tag="PK362_11215"
     CDS             complement(971..>1214)
                     /locus_tag="PK362_11215"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNC75436.1"
                     /translation="SGYLTEMDRNRDDEPQSAFLAQIPLEEGSPLSLDYVRSGGDRTF
                     GWGVATDKDGNAYVSGTFHRERHRFDPFLGRAPARR"
BASE COUNT          242 a          364 c          424 g          184 t
ORIGIN      
        1 ggtcccatcc cccttttcgc cagggaagat cgtggcggcc gttttttttg taccaggcca
       61 acagccgcga tcgaaacagg gcgggtttaa gggcagccat gttccgaaaa ccgtttaagg
      121 gaacgggccg gaaaggccca ctggggggag ttcatggacc cgtcggccgg ggccgggaac
      181 cacggctcgc cggtgtcggc gttttttaaa acgtggattt cctgggccgg catgtagctt
      241 tgcagaagga ggacccacgt ggatccgtcc cgggcgcggg cggcgtcggc cacgatcacc
      301 gcgtggccgg gggaaccgcc ctgaatatag acatcgccga tctccggcgg tccgccggcg
      361 ggaaccgccg cgagttcccg gctgagcgac aaggtgccgg cccatcggaa aagagtgaac
      421 aaataatctt caaaagacgc ccgggaggtg tcggtggacg cccggtgaaa ccaggccacc
      481 cgggagccca ggaccgacgg tcgatcgccc ccggcccaac ggggccaggg acaggggtcg
      541 ccgttggtaa accgaaaaca aacgtccgcc tcccggccgg ccgcgcggag gtattcggcc
      601 cgaaggcgaa taaccgcgtc ggcgcactgc tccaggtcgc gattcccaat gggcatatcg
      661 acgacggcgg cgtggacgtc ctgccgattt ttgagggtgc cgtcatacaa gcgaaccggg
      721 gggcgaccgg atttcaacgg caatcgttgg agccaaccgc cgaaggaatt ttcgggcggc
      781 gaaagacgcg ccgttccccg gggggcttga aagcgggaat ggatcgtttc accctccgcg
      841 gcgtggggcc aggggtaggg ccccggccgg gaggaagcgg ggatcaacag gaaccccagg
      901 aaaaaaacgg cggagtacag gggacggcgc atggggccat tctaccaaag ggggggaagg
      961 gcagtcgttt ttaccgacgg gccggggccc ggccgaggaa cgggtcaaac cggtgtcgct
     1021 cccggtgaaa ggtgccggaa acgtaagcgt tcccgtcctt gtccgtcgcc acgccccagc
     1081 cgaaagtgcg atcgcccccg gagcgcacgt aatccaggga gagcggggat ccctcctcca
     1141 ggggaatttg ggccaaaaag gcgctttggg gttcgtcgtc ccgattccgg tccatttccg
     1201 tcaaataccc cgat
//
LOCUS       DAOQLS010000751         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102744_length_1213_cov_1.367876, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000751 DAOQLS010000000
VERSION     DAOQLS010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102744_length_1213_cov_1.367876"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1213)
                     /gene="carB"
                     /locus_tag="PK362_11220"
     CDS             complement(<1..>1213)
                     /gene="carB"
                     /locus_tag="PK362_11220"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNC75437.1"
                     /translation="GLDASPIHEILLEQSVIGWKEYELEVMRDKADQCVVICSIENLD
                     PMGVHTGDSITVAPAQTLSDPDYQVLRDQAFACIRAIGVETGGSNVQFAVDPKTGHTV
                     IIEMTPRVSRSSALASKATGFPIAKMAALLAVGYRLDEIPNDITKKTPACFEPVIDYV
                     VTKVPRFAFEKFTEADQTLNTSMKSVGEVMAIGRTFRESLQKALRGLEIGRAGLGADG
                     KSIVPKVDELAPRGSDDAEKKKLLEEVEHKLRIPNCDRIFNIKYAFQLGLTVQDICGL
                     SGIDPWFVHQIGMIWDLEKEIKAAGKKMDVPLLRRAKRDGFSDVQLAYLTGLTADAVA
                     KMRKEHVPVTFKKVDTCAAEFPADTPYFYSTYEEEDESLPPSKKERVIVLGGGPNRIG
                     QGIEFDYCCVHA"
BASE COUNT          221 a          341 c          376 g          275 t
ORIGIN      
        1 gcgtggacac agcagtaatc gaactcgatg ccctggccga tgcggttggg gccgccgccc
       61 aaaacgatca cccgctcttt cttcgacgga ggcagggatt cgtcttcttc ttcgtaagtg
      121 gaatagaaat agggcgtgtc ggcgggaaac tcggcggcgc aggtgtcgac tttcttgaac
      181 gtcacgggca catgctcttt gcgcattttc gccacggcgt cggcggtcaa gccggtcagg
      241 taggcgagct ggacgtcgga aaacccgtcc cgtttcgccc ggcggagaag gggcacatcc
      301 atcttttttc cggcggcttt gatttctttt tccaaatccc aaatcatccc gatttgatgg
      361 acaaaccaag ggtcaatgcc ggagaggccg caaatgtcct ggacggtcaa cccgagctga
      421 aaggcgtatt tgatgttgaa gattcggtcg cagttgggga ttcggagttt gtgttccact
      481 tcctccaata attttttctt ttccgcgtcg tctgacccgc gcggggcgag ctcgtccact
      541 ttggggacga tggattttcc atcggcaccg agcccggccc ggccgatttc gagcccgcgc
      601 agggcttttt gcagggattc ccggaacgtg cgcccgatgg ccatgacctc gcccacggat
      661 ttcatgctgg tgttcaaggt ttggtcggcc tcggtgaatt tctcgaaggc gaaacgggga
      721 actttcgtga cgacgtagtc gatgaccggc tcaaaacagg cgggggtctt tttcgtgatg
      781 tcgttgggga tttcgtcgag ccggtagccc acggccaaga gcgcggccat tttggcgatg
      841 gggaaccccg tggctttgga ggccagcgcc gacgagcggg acacgcgggg ggtcatctcg
      901 atgatcaccg tgtggccggt tttcggatcc acggcaaact ggacgttgga gccgccggtt
      961 tccacgccga tggcgcggat gcaggcgaac gcctggtcgc gcaagacctg atagtccgga
     1021 tcggacaggg tttgcgccgg ggccacggta atggaatccc ccgtgtggac ccccatgggg
     1081 tctaagttct caatggaaca gatcaccacg cactggtcgg ccttatcccg catcacttcc
     1141 agctcgtatt ctttccagcc gatgacgctt tgttccaaca aaatttcatg gatcgggctg
     1201 gcgtccaggc ccc
//
LOCUS       DAOQLS010000752         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_102768_length_1212_cov_4.146067, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000752 DAOQLS010000000
VERSION     DAOQLS010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102768_length_1212_cov_4.146067"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK362_11225"
     CDS             complement(<1..221)
                     /locus_tag="PK362_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75438.1"
                     /translation="MIKINLLPREIYADKARRQLASLGVGIGVLIAAVLLGFYGLLKK
                     KEISLVKQLSEARMEETKYQAIANEVKQL"
     gene            complement(240..>1212)
                     /gene="pilM"
                     /locus_tag="PK362_11230"
     CDS             complement(240..>1212)
                     /gene="pilM"
                     /locus_tag="PK362_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNC75439.1"
                     /translation="LPNAGPDVAGPERRVQAISLLKEYVAKQKDSISKNAVFSVSGNS
                     VIVRFVKFPKMSRDDLSKMIVIEAEPYIPFAIPEVNIDFNILGDTVEEGQKKMETILV
                     AAKKEIINGRLEIIQQSGMTPSLIDVDAFALQNAYELNVGANAKETALLVHIGAFVTT
                     MTIIESGVPKVVRDVFIAGNTVTKALQRNFQVDAKKADDMKAKAVILATQEEREKAMA
                     DQNKEVLQMSTVILPVMKDLLAEIQRSLDFYLSQGTDRQVTRVLLSGGTSRLGNLVNY
                     LAQELRLPVEMFDPFARIDGAQSIAPDMRPLFAVAVGLALRREGDIV"
BASE COUNT          243 a          339 c          356 g          274 t
ORIGIN      
        1 tccagctgct tgacttcgtt ggcgatggcc tggtacttgg tttcctccat ccgggcttcg
       61 ctcaactgct tcaccaagga gatttccttt tttttcagaa gaccgtagaa gccgagcaaa
      121 acggcggcga tgagaacccc gatgccgacg ccgagggacg ccagttgccg gcgggccttg
      181 tcggcgtaaa tttcgcgggg gagaaggttg atcttaatca tgaaaaaggg gcccgaaggc
      241 tagacgatgt ccccttcccg ccgcagagcc aaaccgacgg ccacggcgaa cagcggacgc
      301 atgtcgggcg cgatggattg ggcgccgtcg atgcgggcga acggatcgaa catttccacc
      361 ggaagtcgca gctcctgggc caaatagttg accaaattcc ccaggcggct cgtccccccc
      421 gacaacaaaa cccgcgtcac ttggcggtcc gtcccctgcg aaaggtaaaa atccaaggag
      481 cgttgaattt ccgccagcag gtccttcatg acgggcaaaa tcacggtgga catctgaagg
      541 acttccttgt tttgatccgc catggccttc tcgcgctctt cctgggtcgc gagaatcacg
      601 gccttggctt tcatgtcgtc ggctttcttg gcgtccacct gaaaattccg ctgaagggct
      661 ttggtcacgg tgttcccggc gataaacacg tcgcgaacca ccttgggcac gcccgactcg
      721 atgatggtca tggtcgtgac aaaggccccg atgtggacca gaagcgccgt ttctttcgcg
      781 ttggccccca cgttcaattc atacgcgttt tgaagggcga aggcgtccac gtcgatcagc
      841 gaaggggtca tgccgctttg ctgaataatc tccaaccgcc cgttgatgat ttcttttttg
      901 gccgccacca aaatggtttc catttttttc tggccttcct cgacggtgtc gccgaggatg
      961 ttgaaatcga tgttgacttc ggggatggcg aaggggatgt agggctcggc ctcgatgacg
     1021 atcattttgg aaaggtcgtc ccgggacatc ttcggaaact tcacgaaacg aacgatgacg
     1081 gagttgccgg agaccgaaaa gacggcgttt ttggaaatgc tgtctttttg cttggcgacg
     1141 tattccttga gaaggctgat ggcctggacg cgccgttccg gaccggccac gtccgggccc
     1201 gcgttgggaa gg
//
LOCUS       DAOQLS010000753         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_103313_length_1209_cov_2.980069, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000753 DAOQLS010000000
VERSION     DAOQLS010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103313_length_1209_cov_2.980069"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PK362_11235"
     CDS             <1..1154
                     /locus_tag="PK362_11235"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNC75440.1"
                     /translation="NDRRYILFCRAALEAARAAGFAPDVVHAHDWQTGLLPTYLATSY
                     RDDPFFQNTATVFSIHNIAYQGHFPKETLLAAGLDWSWFTADRLEYFDGFNFLKAALV
                     TSDVLSTVSPTYAVEVQSGEEFGRGMEGVLQTRSRDFFGVLNGIDNAVWNPAEDAMIA
                     RPFSARALRGRADCKRDLQRQTGLAEDPAAPLLGMVSRLDPQKGFDILLDVLEPFIEE
                     GAQAVFLGQGSPVYQATLRTLSRKHPGRVASSTDFNETLAHKIYAGADIFLMPSRFEP
                     CGLGQMIALRYGAVPVVTPTGGLKDTVVPVSRDGKKGDGFVAAATETGAYRRALESAV
                     DRFRRDPAGWKRILKRGMARVFDWGDSVPKYEEIYRRALARRADRIGPP"
BASE COUNT          192 a          397 c          399 g          221 t
ORIGIN      
        1 acaacgaccg ccgctacatc ctcttttgcc gcgccgccct ggaggccgcc cgggccgccg
       61 ggttcgcgcc cgacgtggtt cacgcccacg attggcagac cggtttgctg ccgacctatt
      121 tggcgacgtc gtaccgggac gacccttttt tccaaaacac cgccacggtc ttcagcatcc
      181 acaacatcgc ctaccagggg cattttccga aggaaacctt gctcgccgcc gggttggatt
      241 ggtcctggtt caccgcggac cgcctggaat atttcgacgg gttcaatttt ttgaaagccg
      301 ctttggtgac gtccgatgtc ctttccaccg tgagccccac ctacgcggtg gaagtccaat
      361 cgggggagga attcggccgg ggcatggaag gggtgcttca aacgcgctcc cgggattttt
      421 tcggcgtgct gaacggcatc gacaacgccg tctggaaccc ggccgaggac gcgatgatcg
      481 cccgcccctt ctcggcccgg gctctgcggg gtcgcgccga ttgcaaacgg gaccttcaac
      541 gccaaaccgg attggccgag gaccccgcgg cgcccctcct cggcatggtt tcgcggctgg
      601 acccgcaaaa gggattcgac attttgctcg acgtgttgga gcccttcatc gaggaagggg
      661 cccaggccgt cttcctgggg caggggagcc cggtgtacca ggccacgctc cgcacgctgt
      721 cccgcaagca cccgggccgc gtcgcgagtt cgacggactt taacgaaacc ctggcccaca
      781 agatttacgc gggcgcggat atttttttga tgccttcccg gttcgagccc tgcggcctgg
      841 gccaaatgat cgccctccgc tacggcgccg tgcccgtggt gacgcccacg ggcgggctga
      901 aagacaccgt cgttccggtt tcccgcgacg ggaaaaaagg cgacgggttt gtggcggccg
      961 ccacggaaac gggggcttac cgccgggccc tggaatccgc cgtggaccga tttcgccggg
     1021 acccggccgg gtggaaacga attctcaagc ggggcatggc ccgggtcttt gattgggggg
     1081 actccgttcc caaatacgag gagatctatc gccgggcgct ggcgcgccgg gcggatcgga
     1141 tcggccctcc gtgagccttt gggcgcgggg acggcgctgg gcccggcggt ggacccggcc
     1201 ccaggtggg
//
LOCUS       DAOQLS010000754         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_103379_length_1209_cov_2.102253, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000754 DAOQLS010000000
VERSION     DAOQLS010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103379_length_1209_cov_2.102253"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PK362_11240"
     CDS             complement(<1..664)
                     /locus_tag="PK362_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75441.1"
                     /translation="MTSPSELTLDRLALGGDAVGRRPTPGAPTPEVVFVPFGAPGDRV
                     VVGREEKQKTFSRAWISQLVTPSEDRTEPRCPIFYKPGQTPSSACGGCDWQHLAYEKQ
                     TAAKRKLLVESFQRLARIAHPPVEDTVGVTGAEGPWRYRNKVQIPFALGPDGKITGGF
                     TPPGPTPWCPSTIASCSRSFPSGCSRKSGTISATPAFRFTIRRRARGGCGICWCAPPP
                     RGK"
     gene            complement(684..971)
                     /locus_tag="PK362_11245"
     CDS             complement(684..971)
                     /locus_tag="PK362_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HNC75442.1"
                     /translation="MKTREISIQGQVVTGYEIDLPGAPLVLVQAPKGFVMCGYLDVKS
                     ADKFNAAAAVVRGVKTVDDLLAKPVTDVSAAGQTLGIAPGATGRDALLKLL"
BASE COUNT          216 a          364 c          394 g          235 t
ORIGIN      
        1 gcttcccccg tggcggtggt gcgcaccagc aaatgccgca gccacccctc gcccgtcgac
       61 ggattgtaaa ccgaaacgcg ggggtggcgg aaatagtccc ggactttctt gaacagccgg
      121 acggaaagct ccgactgcac gaggcaatcg tcgaagggca ccacggtgtg ggacccgggg
      181 gcgtaaagcc gccggtgatt tttccgtcgg ggccgagggc gaaggggatt tgcaccttgt
      241 tccggtagcg ccaggggcct tcggccccgg tgacgcccac ggtgtcctcc acgggcgggt
      301 gggcgatgcg ggccagtcgc tgaaaagact ccaccaacag cttccgcttg gcggcggttt
      361 gcttttcgta cgccaagtgt tgccagtcgc aaccgccgca agcggaggat ggggtctggc
      421 ccggtttgta aaaaatcggg caacggggtt ccgttcggtc ttctgacggc gtgaccagtt
      481 gggagatcca ggcccgggaa aaggttttct gtttttcctc ccgtcccacg accacccggt
      541 cgccgggggc gccaaaggga acgaaaacga cttcgggggt cggcgcgccg ggggtcggac
      601 ggcggccgac ggcgtcgccg cccagggcga ggcggtccag cgttaattcg gagggggagg
      661 tcatggaacc ggggggggcg cgtttagagg agtttcaaca gggcatcgcg gcccgtcgct
      721 ccgggggcga tgcccagggt ttggcccgcg gcgctcacgt ccgtcacggg cttggccaac
      781 aaatcgtcga cggttttaac gccccgcacg accgcggccg cggcgttgaa tttgtccgcg
      841 gatttcacat cgaggtaacc gcacatcaca aaacctttgg gggcctgaac cagcaccagc
      901 ggcgcgcccg gaaggtcgat ttcgtagccg gtcacgacct gtccttggat cgaaatttca
      961 cgggttttca tggggcctct atgttacatc taatttacag taaattaatc gcctgtttcg
     1021 ttcccgccat tatactaaag acgtcccatg caaaatcctc ttccccgacg atttttagcg
     1081 cggaaatgcg tctccgcctt tttggcggcg gtctacgctt ttacgaacgt tttcgccgtt
     1141 cacgccgccg aaaaaagctt ttggcaggac cgccaagccg cccggggtcg gaaggctccg
     1201 acgctcctg
//
LOCUS       DAOQLS010000755         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_103654_length_1207_cov_2.729167, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000755 DAOQLS010000000
VERSION     DAOQLS010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103654_length_1207_cov_2.729167"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PK362_11250"
     CDS             complement(<1..976)
                     /locus_tag="PK362_11250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNC75443.1"
                     /translation="MAIKKSALYGSLWQSCDELRGGMDASQYKDYVLVLLFVKYISDK
                     YAGDRYAAIQVPPGASFKDMVALKGQPDIGDRINKEIIAPLEKANRITIKTDFNDPAL
                     LGSGKEMVERLTNLIAIFQNPALDFSKNRADGDDILGDAYEYLMRHFATESGKSKGQF
                     YTPAEVSRVMARIIGIGAAGATSTTTVYDPTCGSGSLLLKVGDAAHADVTLFGQEKDE
                     ATSGLARMNMILHNRPGAVIEHGNTLTTPKFKDGETLKTFDYVVANPPFSDKRWSTGL
                     GGENGALHDPFDRFQTFGAPPAKQGDYAYLLHIVRSLKSTGKGACILPH"
BASE COUNT          221 a          321 c          394 g          271 t
ORIGIN      
        1 cgtggggcag gatgcaagcg cccttgccgg tgctcttcag cgaccggacg atgtgcagca
       61 ggtaggcgta gtcgccctgc ttggcgggcg gagcgccgaa ggtctggaag cggtcgaagg
      121 gatcatggag cgcaccgttt tcaccaccca ggccggtact ccagcgcttg tcggaaaaag
      181 gtggattggc gacaacgtag tcgaaggtct tgagggtttc gccatccttg aacttgggcg
      241 tggtcagagt gttgccgtgt tcgatgacgg cgcccggcct gttgtgcagg atcatgttca
      301 tccgcgccag gccgctggtg gcttcgtcct tttcctggcc aaacagggtg acgtcggcgt
      361 gggccgcgtc gcccaccttc agcaaaagcg atccggagcc gcaggtcggg tcgtagacgg
      421 tggtggtgct ggtggcgccg gcggcgccga tgccgatgat cctggccatg acccggctga
      481 cctcggccgg ggtgtagaac tgccccttgg acttgcccga ctcggtggcg aagtgccgca
      541 tcaggtattc gtaggcgtcc cccaggatat cgtcgccgtc ggcccggttt ttcgagaaat
      601 ccagggccgg gttctggaag atggcgatga ggttggtgag ccgctccacc atctccttgc
      661 cggaacccag cagggccggg tcgttgaagt cggtcttgat ggtgatgcgg ttggccttct
      721 ccagcggggc gatgatctct ttgttgatcc tgtcgccgat gtccggctgg cccttgaggg
      781 cgaccatgtc tttgaaactg gccccgggcg gaacttggat ggcggcgtag cggtccccgg
      841 cgtatttgtc gctgatgtat ttgacgaaca agaggacgag gacgtagtcc ttgtactggc
      901 tggcgtccat cccgccgcgc aactcgtcgc aggattgcca aagggaaccg tagagggcgc
      961 tttttttaat ggccatatgg gttatgagaa attatttgcc acgaatttct actgaactcc
     1021 aagatcgttt aaaactggag tttatagtat acccgattcc ctctaggcaa taagctggtg
     1081 attccaggcc aacattaaag gtattagggg acgtcatgcc cccctaatta cagcgaaaac
     1141 ccaggaaatc cccggtgttt gggttacggg gatttgttaa aaaaaccgat tgctcggcac
     1201 cggggac
//
LOCUS       DAOQLS010000756         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_103748_length_1206_cov_4.668983, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000756 DAOQLS010000000
VERSION     DAOQLS010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103748_length_1206_cov_4.668983"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PK362_11255"
     CDS             complement(<1..542)
                     /locus_tag="PK362_11255"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNC75444.1"
                     /translation="MELTNGYFSLNGRPFFLYSGEVHYFRIPKTQWRARLTALKKAGF
                     NTVSTYIPWSWHEPEEGRIDLTGETHPERDVMTFLDTAKSLGLFVSARVGPVSNAELV
                     NEGIPGWLLSGYPDVFVTGRRNVGNLPHVTILSYLHPVFQEKVSRWYDALLPGVVQRQ
                     TTRGGNVLAVQLCNEVAMVH"
     gene            628..>1206
                     /locus_tag="PK362_11260"
     CDS             628..>1206
                     /locus_tag="PK362_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNC75445.1"
                     /translation="MKRIAKLAGTGALIFTLAYLGFTPAGLGFFGQPARVPTLAELNN
                     ANPGTTAYMEIRRREAEALGKKWRPRFSWVSLDRVSPALVNAVLAAEDSTFYQHHGIE
                     WGLTRDALLENLRRGEKARGASTITQQVARNLYLSPQKSYLRKIREMILAQRLEQTLT
                     KRRILEIYLNIAEWGPGVFGAEAAAQTYFGKPA"
BASE COUNT          253 a          371 c          364 g          218 t
ORIGIN      
        1 cagtgaacca tggcgacttc gttgcaaagt tgaacggcca acacgttgcc gccccgggtg
       61 gtttgccgct ggacaacccc gggcaaaagg gcgtcgtacc accgggacac cttttcctga
      121 aacacggggt gcaggtagga caaaatcgtc acgtggggca aattgccgac gttccggcgc
      181 cccgtcacga agacgtcggg ataccccgag agcagccagc cgggaattcc ctcgttgacc
      241 aattccgcgt tggacacggg tcccacccgg gccgacacga acaatcccag ggatttggcc
      301 gtatccagga aggtcatcac gtcccgctcg gggtgggttt cccccgtcag gtcgatgcgg
      361 ccttcctcgg gctcgtgcca gctccagggg atgtaggtgc tgacggtgtt gaacccggcc
      421 tttttgaggg ccgtcaaccg cgcgcgccat tgggttttgg ggatgcggaa ataatgcact
      481 tcgcccgagt aaaggaaaaa cgggcggccg ttaagggaaa aatagccgtt ggtcagttcc
      541 attgtaagag cgtgtccgcg gttcggagag cgattacact agcaaagaat ttcacagggc
      601 ggaaggcggt ttgttacaat ggacggcatg aagagaatcg ccaaattggc cggaaccggc
      661 gcgttgattt tcaccttggc ctatctcggg tttacgcccg cgggcctcgg gtttttcgga
      721 cagcccgccc gggttcccac cctggccgaa ctgaacaacg cgaaccccgg caccacggcg
      781 tacatggaaa ttcggcggcg ggaggccgag gccctgggca aaaaatggcg cccccgtttc
      841 tcctgggtat ccctggaccg ggtttccccc gcgctcgtga acgccgtcct ggccgccgag
      901 gacagcacgt tttaccagca ccacggcatc gaatggggcc tcacccgcga cgcgctgttg
      961 gaaaaccttc gccgggggga aaaggcccgg ggcgccagca ccatcaccca gcaggtcgcc
     1021 cgcaaccttt atttgtcccc ccaaaaaagt tacctgcgaa aaatccgcga aatgattttg
     1081 gcccaacgcc tggaacaaac tttgaccaag cggcgaattc tggaaatcta cctgaacatc
     1141 gccgaatggg gtcccggcgt cttcggcgcc gaggccgcgg cccaaaccta tttcggcaaa
     1201 ccggcc
//
LOCUS       DAOQLS010000757         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_103793_length_1206_cov_3.100782, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000757 DAOQLS010000000
VERSION     DAOQLS010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103793_length_1206_cov_3.100782"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1206)
                     /locus_tag="PK362_11265"
     CDS             complement(<1..>1206)
                     /locus_tag="PK362_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75446.1"
                     /translation="KQDADAFRITLDDADTDPVIVRAATPRPTFRANAPLAETPLRIL
                     RREDGPVETRITVELPARNVLLAEVVLAAEDAAFQRPVRVVRRVRSGDENVFDEILGA
                     ESLCRWPGEDGKTFERLSIPVARRCDSRDVDIVVANGDNPPLKITSLGARVAPTTLVF
                     NATEPGTYRLILGNPAAGPVRYDLDGLRDRLDKKQRILAEAGPLQSNPAYAPPAVAAD
                     LAESAAALDPKGWRWKSPVRIEKTGVQRLELSAAAVAGARSDGGDLRLLRDGRQVPFV
                     VDRRVVYRVLAPTVKATDAEGGRNSVWEIQLPVRGLPVSHLEIDAPEKLFSRRVVVEE
                     NRNVPGAGTERTVLGGAQWFRRGETTRLLVPLTIAPSGDRLRLTVGNGDNAPLSLANF
                     RVPYRTYALV"
BASE COUNT          193 a          402 c          419 g          192 t
ORIGIN      
        1 accagggcgt aggtccggta agggacgcgg aaattggcca gggacagggg cgcgttgtcg
       61 ccgttgccca ccgtcaggcg aagtcgatcg ccgctgggcg cgatggtcag gggaaccagc
      121 aggcgggtgg tttccccccg ccggaaccac tgggcaccgc ccaggaccgt gcgttcggtt
      181 cccgcgccgg ggacgttgcg gttttcctcg accacgaccc ggcgggaaaa caatttttcc
      241 ggggcgtcga tctccaaatg ggaaacgggc aatccccgga ccgggagttg aatctcccac
      301 acgctgtttc gcccgccctc cgcatccgtc gccttcacgg tcggcgcgag aacccgatag
      361 acgacccggc gatccacgac aaaggggact tggcggccgt cgcgcaacag gcgcaggtcg
      421 ccgccgtcgg accgggcgcc ggccacggca gcggcggaca attccaaacg ctgaacgccg
      481 gttttttcaa tccgcacggg gcttttccat cgccaccctt tgggatccag ggcggccgcg
      541 ctttcggcca aatcggccgc caccgccgga ggggcgtaag cgggattcga ttgaaggggc
      601 cccgcctccg ccagaatccg ctgtttcttg tccaagcggt cccgaaggcc gtcgagatcg
      661 tagcgaaccg gccccgcggc ggggtttccg agaatcagcc ggtaggtccc gggttccgtg
      721 gcgttgaaca ccagggtcgt cggcgccacc cgggccccga gggaggtgat cttcagcggg
      781 ggattgtcgc cgttggcgac cacgatgtcc acgtcccggg aatcgcaccg gcgggccacg
      841 ggaatggaca gccgttcgaa agtctttccg tcctccccgg gccaacggca gagggattcg
      901 gcgcccagga tttcatcgaa gacgttttcg tcgccggaac ggacccggcg taccacccgc
      961 acgggcctct ggaaagccgc gtcctccgcc gccagcacga cctcggccaa caggacgttg
     1021 cgggccggga gctccaccgt gatccgggtt tccaccgggc cgtcttcccg gcgaagaatt
     1081 cgcagcgggg tttcggcgag gggcgcgttg gcgcgaaagg tcggccgggg ggtcgcggcc
     1141 cggacgatca ccgggtcggt gtccgcgtcg tccagggtga ttcgaaaggc gtcggcgtcc
     1201 tgtttc
//
LOCUS       DAOQLS010000758         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_103910_length_1205_cov_5.924348, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000758 DAOQLS010000000
VERSION     DAOQLS010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103910_length_1205_cov_5.924348"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PK362_11270"
     CDS             complement(<1..918)
                     /locus_tag="PK362_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC75447.1"
                     /translation="MLTVQNLHKAFAAQVIFDGANLQLNAGDRYALMGPNGAGKSTLF
                     KILRGLETPDDGIVSFPRGLKIGYLPQETAELGEGTVLEETLAGEGDPGDEKRAAEAK
                     KTLMGLGFRVTDFDRIATTLSGGWRMRVAIARLLLQAPDLLLLDEPTNHLDLQSLFWF
                     QEYLQNSKSSLLLISHDRAFVNAVVHGILDLREKKIFRYVGDYEHFLAAREGEAERLM
                     AAYKKQQKDIAEAQDFINRFRAQAAKAPQVQSRIKTLEKMERIEIPPEIKKVKIQFPQ
                     PSKPGVRVLALKNIHKAYGDLKVYDGLDFE"
BASE COUNT          212 a          362 c          357 g          274 t
ORIGIN      
        1 ctcgaaatcc agcccgtcgt aaactttcaa atcgccatag gctttgtgaa tgtttttcaa
       61 ggccaacacg cggacgcccg gcttggacgg ttggggaaat tggattttga ctttcttgat
      121 ttccggcggg atctcgatcc gttccatttt ctccagcgtc ttgatgcggc tttgaacctg
      181 gggcgccttg gccgcctggg cgcggaatcg gttgatgaaa tcctgggcct cggcgatgtc
      241 cttttgctgt ttcttgtagg ccgccatcaa ccgttcggcc tcgccctccc gggcggccag
      301 gaaatgctcg tagtcgccga cgtatcggaa aatctttttc tcccgcaggt ccaaaatccc
      361 gtggaccacg gcgttcacga aggcccggtc gtgcgagatg agcaggagcg aacttttcga
      421 gttttgaagg tattcctgaa accaaaaaag ggattgaaga tccagatggt tggtcggttc
      481 gtccagaagc agcaaatccg gcgcctggag gagcaaacgc gcgatggcca cgcgcatgcg
      541 ccacccgccg gacagggtgg tcgcgattcg gtcgaagtcg gtcacccgaa agccgaggcc
      601 catcagggtc tttttggctt ccgccgcgcg tttttcgtcg ccgggatccc cctccccggc
      661 cagggtttcc tccaagacgg tgccctcgcc cagttcggcg gtttcctggg gcaggtagcc
      721 gatcttgagc ccccggggaa aggagacgat gccgtcgtcc ggggtctcca acccccgaag
      781 gattttaaaa agggtggatt tccccgcccc gttggggccc atcagagcgt accggtcccc
      841 ggcgttcaat tgcaggttgg ccccgtcgaa aatcacctgg gcggcgaagg ctttgtggag
      901 gttttgaacc gtgagcatgg ttaaaaaaaa ccggagcggc tcgccgctcc ggcggtgttc
      961 ctttcgatcg atttcttccg ggggccattg aacgggcgcc ccgcgccggt ggcatggaat
     1021 tcgccacttt tcacacgtcc gacttcgtcg gacgattcca ccgtacggcc tcctgccttt
     1081 ttacgcaggc gcctcgtctc ttgctaaatc tcggggtgac tggacttgaa cgggcgcccc
     1141 gcgccggtga catggaattc gccacttttc acacgtccga cttcgtcgga cgattccacc
     1201 gtacg
//
LOCUS       DAOQLS010000759         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_104089_length_1204_cov_4.832898, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000759 DAOQLS010000000
VERSION     DAOQLS010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104089_length_1204_cov_4.832898"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..198)
                     /locus_tag="PK362_11275"
     CDS             complement(31..198)
                     /locus_tag="PK362_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75448.1"
                     /translation="MTDPGKLIELDVKPRSRIIVADDEQDIATLIEDWLSDTYEVTVA
                     LNGKAAIQKAI"
     gene            complement(220..>1204)
                     /locus_tag="PK362_11280"
     CDS             complement(220..>1204)
                     /locus_tag="PK362_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNC75449.1"
                     /translation="DGVLIQTNLEKMTPDMCQRLIYSLLSDVQKQRFESENELDISFG
                     IKGLGRVRMNVFRQRGSVGAALRSVPSRFMSFEELGLPPVINDVVNLPRGLVLVTGPT
                     GSGKSTTLASLIDYINENRQCHIVTIEDPIEYIHFHKQSLVNQRELGTDTQSFTSALK
                     YVLRQDPDVILVGEMRDAETIAAAMTIAETGHLVFSTLHTNDAAGTVNRILDSFPPER
                     QEQARSQLSFTLQAILSQALLMHASGSGRVLAAELLLVTPAVRNLVREQKVEQIYLAM
                     QTGSKSGMQTMNQCLLGLVQRGQITHATALEYSNDVDELRKALPKETSTGF"
BASE COUNT          223 a          341 c          377 g          263 t
ORIGIN      
        1 gatgtcgcag agaatgacgt cggggcgctg ctaaatggct ttttggatgg ccgcttttcc
       61 gttcaaggcc acggtcacct cgtaggtatc ggacagccaa tcttcgatga gcgtcgcgat
      121 gtcctgctca tcgtccgcca cgatgatccg ggagcggggc ttgacgtcaa gctcgatgag
      181 tttgccgggg tccgtcatgg gcgtgggcgg gggacgggtt tagaatcccg tcgatgtctc
      241 cttcggaagc gccttgcgca attcgtccac gtcgttggaa tattccaacg cggtggcgtg
      301 ggtgatctgt ccccgctgaa ccagccccag caggcattgg ttcatggttt gcatgccgga
      361 cttgctgccc gtctgcatcg cgaggtaaat ctgctccact ttttgttccc gcacgaggtt
      421 tcggaccgcg ggggtcacca gcaggagttc ggccgccaac acgcggcccg aacccgaggc
      481 gtgcatgagc agggcctggc tcaagatcgc ttgaagcgta aaggacaact ggctgcgggc
      541 ctgctcctgg cgttccggcg gaaaggaatc caaaatccgg ttgacggtcc ccgccgcgtc
      601 gttggtgtga agggtcgaaa agaccaggtg gcccgtttcc gcgatggtca tggccgccgc
      661 gatcgtttcg gcgtcccgca tttcgcccac cagaataacg tcggggtcct gccgaagaac
      721 gtatttcagg gccgaggtga aactttgggt gtcggtgccg agctcccgct ggttgaccag
      781 cgactgtttg tggaaatgga tgtattcgat gggatcctcg atggtcacga tgtggcactg
      841 gcggttttcg ttgatgtaat cgatgaggct ggccagcgtg gtggatttcc ccgacccggt
      901 ggggcccgtc acaaggacca acccccgggg aaggttgacc acgtcgttga tgacgggcgg
      961 caatcccaat tcctcaaaac tcatgaaccg ggacgggacc gaccgcaagg ccgcgcccac
     1021 gcttccccgc tggcggaaca cgttcatccg gacgcgcccc aaccctttga tcccgaaaga
     1081 aatgtccagc tcgttttcgg attcaaaccg ctgtttttgc acgtcggaca ggagggaata
     1141 aatgagccgt tggcacatgt cgggggtcat tttttccaaa ttggtttgaa tcaaaacccc
     1201 gtcc
//
LOCUS       DAOQLS010000760         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_104303_length_1203_cov_3.216899, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000760 DAOQLS010000000
VERSION     DAOQLS010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104303_length_1203_cov_3.216899"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PK362_11285"
     CDS             <1..582
                     /locus_tag="PK362_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC75450.1"
                     /translation="RTSEAPPLLDPQTSVVSVVSRGEIIQVDMNEKKPELRFPVPLEE
                     LVEWSLLENGHYALVGYDKEAKTVAEVGPGGDVLWRYHDTAGKDKWLAHPPISGPGGL
                     VYVLTDQRVVAIKKGATVWAFNFGGTVQAGFACALSDGSVLATHDDTLQHLDGNGKAL
                     FTLKLDKPIVSPPAVDQGGSIYVATDAQLLRID"
     gene            complement(599..>1203)
                     /locus_tag="PK362_11290"
     CDS             complement(599..>1203)
                     /locus_tag="PK362_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75451.1"
                     /translation="SNFPMSILRRREVFGACILTAATVFVFSHPAPAKEKRTMIQFNG
                     TTYEHRWSKSGQNEFTPAGQEDLSKWTEMVTILDYDRVNNGEDLAGVANQVYANYQNA
                     GKIIKVNSKPRTETAPAEHFIAAALGDPNYLELVFARLFIHDGKGAAVVVSHRIYGAK
                     VGPAAGEWLEKNGASVEKALMEWKGLPTLAALRALPQSAN"
BASE COUNT          229 a          332 c          386 g          256 t
ORIGIN      
        1 cgcacgtccg aggcgccgcc cctgctcgat ccccaaacat ccgtggtgtc ggtggtttcc
       61 cggggggaaa tcattcaagt ggatatgaac gagaaaaaac cggaacttcg attcccggtc
      121 cccctggaag aactcgtgga gtggagcctt ttggaaaacg gtcattacgc cctggtcggg
      181 tatgacaaag aagcgaagac ggtggcggag gtcggcccgg gcggggacgt cctatggcgc
      241 taccacgaca cggcgggaaa agacaagtgg ctggcccacc cgcctatttc cggcccgggc
      301 ggcctggtgt atgtcctgac ggaccagcgc gtggtggcga tcaaaaaggg ggcgacggtg
      361 tgggccttca attttggcgg gaccgttcag gcggggtttg cctgcgccct gtccgacggg
      421 tcggtgctgg cgacccacga cgacactctt caacacttgg atgggaacgg caaagcgcta
      481 tttaccctga aactcgacaa acccatcgtg agcccgccgg ccgttgatca aggcgggtcg
      541 atttatgtgg ccaccgacgc ccaactgcta aggatcgatt gacgcgagct tttaaaggtt
      601 agttggcgct ctggggcagg gccctcaagg cggcgagggt gggcaggcct ttccattcca
      661 tcaacgcttt ttccaccgaa gctccgtttt tctccagcca ttcgccggcg gcgggtccga
      721 ctttggcgcc gtagatccgg tgggagacga ccacggccgc ccctttcccg tcgtgaatga
      781 aaaggcgggc gaagaccagc tcgagataat tcgggtcgcc cagggcggcg gcgatgaaat
      841 gctcggcggg cgcggtttcg gttcggggtt tggaattgac tttgatgatc tttcccgcgt
      901 tctggtaatt ggcgtacacc tggttggcaa ccccggcaag gtcctccccg ttgttcaccc
      961 gatcgtaatc gaggatggtc accatctccg tccacttcga caggtcctcc tgtccggcgg
     1021 gggtgaattc gttttgaccg gacttggacc atcggtgttc gtaggtggtt ccgttgaatt
     1081 ggatcatcgt tcgtttctcc ttggcgggag cgggatggga aaaaacaaat accgttgcgg
     1141 cggtcaagat gcacgcgccg aaaacttccc ttcgtcttag gatggacatg ggaaaatttg
     1201 agg
//
LOCUS       DAOQLS010000761         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_104859_length_1200_cov_2.558079, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000761 DAOQLS010000000
VERSION     DAOQLS010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104859_length_1200_cov_2.558079"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PK362_11295"
     CDS             <1..684
                     /locus_tag="PK362_11295"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNC75452.1"
                     /translation="ALLNALELAVLTTPEKDGRVHLTMSVAEASTQRRSLRYDKSADE
                     HYDTISAFIKSLRGSDPDAAIYWMAKMLAAGEDPRFVARRLLVAAAEDVGNADPRALL
                     IANAVFDVVEKLGMPEARIPLAQAAAYVALAPKSNAAYAAINAAWAEVENGPRREVPD
                     ALKDANRDAESRGHGLGYQYPHDFPKHFVPQTYMTQWKPFYRPTELGDERRLKEHYEA
                     LWPERRPLP"
     gene            681..>1200
                     /locus_tag="PK362_11300"
     CDS             681..>1200
                     /locus_tag="PK362_11300"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNC75453.1"
                     /translation="MIGQDVRHKDFLRRHFRDIVRGLPTRRRSREARKAADRLARLPV
                     FVRAPAVAVYVARPGEMDTGPLIALSRRAGKTVLAPIVDPSTQTLRFAVWSGRGKPNV
                     YGIPEPRTDRRSERGLGGRDLLVVPGTAFTPVGDRLGGGGGYYDRFLARSSVQTAALA
                     FPEQIATRLPARP"
BASE COUNT          208 a          395 c          390 g          207 t
ORIGIN      
        1 gccctgctga acgccctcga actggccgtc ctgacgaccc cggaaaaaga cggacgcgtc
       61 catctcacga tgtccgtggc ggaagcctcc acgcaaaggc gctctcttcg ctacgacaag
      121 tcggccgacg aacattacga cacgatttcg gcgtttatca aatccctgag ggggtcggat
      181 cccgacgcgg cgatttattg gatggccaaa atgttggccg ccggggagga cccgcggttc
      241 gtcgcccgcc ggctgttggt ggccgccgcc gaagacgtgg gcaacgcgga tccccgggcg
      301 ctgctgatcg ccaacgcggt ttttgacgtc gttgaaaaac tgggcatgcc ggaggcgcgc
      361 attcccctgg cccaggcggc cgcttacgtg gcgctggcgc cgaagtccaa cgccgcgtac
      421 gcggccatca acgccgcctg ggcggaggtg gaaaacggcc cccggcgcga agtcccggac
      481 gcgctcaaag acgccaaccg ggacgccgaa agccgcgggc acggcctggg gtatcaatac
      541 ccccacgatt ttcccaaaca cttcgtcccc caaacctata tgacccaatg gaaacccttt
      601 taccggccga cggaactggg cgacgagcgg cgcttgaagg aacattacga agccctgtgg
      661 cccgagcgac ggccccttcc atgatcggcc aggatgttcg ccacaaggat tttcttcgac
      721 gacatttccg ggacattgtt cgcggattgc cgactcgtcg tcgttcccgg gaggcgcgga
      781 aagcggcgga ccgactggcc cgcctgcccg ttttcgttcg agcgcccgcc gtggcggtgt
      841 acgtggcgcg tcccggcgaa atggacaccg gcccgttgat cgccttgagc cgtcgagccg
      901 gcaaaaccgt tttggcgccc atcgtcgacc cgtccaccca aacccttcgc ttcgccgtct
      961 ggtcggggcg gggaaagccc aacgtttacg gaatcccgga accgcggacc gaccgtcggt
     1021 cggagcgggg tttggggggc cgggatttgc tggttgttcc cggaacggcg tttacgccgg
     1081 tcggcgatcg cttgggcggg ggcgggggat actacgatcg atttctggcg cgttcgtcgg
     1141 ttcaaacggc ggcgttggcc ttcccggaac aaatcgcgac gcgcttgccg gcgcgacccc
//
LOCUS       DAOQLS010000762         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_105259_length_1197_cov_4.679510, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000762 DAOQLS010000000
VERSION     DAOQLS010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105259_length_1197_cov_4.679510"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PK362_11305"
     CDS             <1..269
                     /locus_tag="PK362_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75454.1"
                     /translation="PPTSRRGFCTWNCRDGPGRPGDLPPGSRRLLAVLTEDPVGIDAL
                     AARAGLSPADVATGLLHLELSGWARELPGKNYVRTERALPAVPS"
     gene            281..>1197
                     /locus_tag="PK362_11310"
     CDS             281..>1197
                     /locus_tag="PK362_11310"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNC75455.1"
                     /translation="MAKSLLIVESPTKEKTIGKMLGKGFVIRSSYGHIRDLPSKELGV
                     DPEKNFEPSYVILPKAKKILPELIRLAKDADKVYLATDYDREGEAIAWHLTHALKLDL
                     ARTKRITFHEITKEALQDAVKRPRDIDDALVNAQVARRVLDRLVGYRLSPLLWKKIRR
                     GLSAGRVQSVAVRLICAREEEIEKFKAEEYWTLTALLEKSGQAFAAGLFAEGETKFTK
                     FSFRAKEPLDRVLEKLRGATYVVKSVEPKERRRSPTPPFTTASLQQDSSRRLHFSASR
                     TMVVAQQLYEGIEVKAGEGPEGLITYMRT"
BASE COUNT          237 a          387 c          364 g          209 t
ORIGIN      
        1 gcccgccgac gtcgcgacgg ggcttttgca cctggaattg tcgggatggg cccggccgcc
       61 ccggggatct gccgcccggg tcccgccggc ttctcgcggt cctgacggag gatcccgtgg
      121 ggatcgacgc gctggccgcc cgggccggcc tctcgcccgc cgacgtcgcg acggggcttt
      181 tgcacctgga attgtcggga tgggcccggg aactgccggg aaaaaactac gtgcgaacgg
      241 agcgggcgtt gcccgccgtt ccctcttaaa aggatcgttc atggccaaat ccctcttgat
      301 cgtggagtcg ccgaccaagg aaaaaaccat cggcaaaatg ctggggaagg ggttcgtcat
      361 ccgaagctcc tacggacaca tccgcgatct tccctccaag gaactgggcg tcgaccccga
      421 gaaaaatttt gagccctctt acgtgatatt gcccaaggcc aaaaaaattc ttccggagtt
      481 gatccggttg gccaaagacg ccgacaaagt ctatctcgcc acggactacg accgcgaagg
      541 cgaggccatc gcgtggcatt tgacccacgc cctgaaactc gatttggcgc ggacgaagcg
      601 gatcaccttc cacgaaatca ccaaggaagc tcttcaggac gccgtcaagc gcccccggga
      661 catcgacgac gccctggtca acgcccaggt cgcccgccgg gtgctggacc gcctcgtggg
      721 ctatcgattg tcgccccttc tgtggaaaaa aatacggcgc ggcctttccg ccgggcgggt
      781 tcagtccgtg gccgtccgcc tgatctgtgc ccgggaagag gaaatcgaaa agtttaaagc
      841 cgaggaatac tggaccctga cggccctgtt ggagaaatcc gggcaagcct tcgccgccgg
      901 tttgttcgcg gagggcgaga cgaagttcac caaattttcg ttccgcgcca aggaaccctt
      961 ggaccgggtg ttggagaagc tccggggagc gacctacgtc gttaaatccg tcgaacccaa
     1021 ggagcgccgc cgatcgccga cgccgccctt cacgaccgcg agccttcagc aggattcctc
     1081 ccgccggctc catttctcgg cctcccgcac catggtggtc gcccagcaac tttacgaagg
     1141 catcgaggtg aaggccgggg aaggccccga aggtctcatc acgtacatgc gcaccga
//
LOCUS       DAOQLS010000763         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_105655_length_1195_cov_3.578070, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000763 DAOQLS010000000
VERSION     DAOQLS010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105655_length_1195_cov_3.578070"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK362_11315"
     CDS             complement(<1..264)
                     /locus_tag="PK362_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75456.1"
                     /translation="MARWTFRRWALALVLLGLGAFRIRAEETFVVEDSTVSLTTATLT
                     CDYLEFRSTDNAVIARGNAVLLSSTTRLEADDLTLYLSSRVAQA"
     gene            complement(268..>1195)
                     /locus_tag="PK362_11320"
     CDS             complement(268..>1195)
                     /locus_tag="PK362_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC75457.1"
                     /translation="KRRKRDTMRNGWRLGIDLGGTQVKLALVDERGGIRELQRVDTDR
                     RPTALVAALKKAVTPWLRRPLRGTGVGVAGDVDPVKGVVRFAPNLRWNNVPLAALFKK
                     HRFPRPLFVENDANAAAWGAYHLEFRRRVQNLVAFTLGTGVGGGLILGGRLYRGASGT
                     AGELGHMVIEADGAPCACGNRGCLEAYLGGAAMVAWARREYRRTGRAAGELDPKILED
                     RARAGDRIARAAWRRASRAFGVALANVLNIFNPEAILLAGGVARGAPLFLPAARKVMA
                     ARAFGTPARAVQITVSRRSADLGVAGAALLVP"
BASE COUNT          207 a          414 c          399 g          175 t
ORIGIN      
        1 ggcctgggcg acccggctgg agaggtaaag ggtcagatca tcggcctcca gccgcgtggt
       61 gctcgagagc aacaccgcgt tcccccgggc gatcacggcg ttgtcggtgg aacggaattc
      121 caggtaatcg caggtgaggg tcgccgtggt gagggaaacg gtggaatcct ccaccacaaa
      181 ggtttcctcc gcgcggattc ggaacgctcc cagacccaga aggaccaggg ccaacgccca
      241 ccgccggaac gtccatcgcg ccatggatta agggacgaga agcgccgcgc cggccacgcc
      301 caggtccgcg cttcgacggg ataccgtgat ttggacggcg cgggcgggcg tcccgaaggc
      361 acgcgccgcc atgacttttc gggccgcggg aaggaacagg ggcgcgcccc gggccacccc
      421 ccccgcgagc agaatcgctt cggggttgaa tatattgaga acgttggcca aggcgacgcc
      481 gaaggcccgg gaggcccgcc gccaggcggc ccgggcgatc cggtcgccgg cccgggcgcg
      541 gtcttccaaa attttcgggt ccaattcgcc ggccgcgcgg cccgttcgac ggtattcgcg
      601 ccgggcccag gccaccatcg ccgccccgcc caaataggct tccaggcagc cgcgattgcc
      661 gcaggcgcaa ggagcgccgt cggcttcgat caccatgtgg cccagttccc cggccgtgcc
      721 ggaggctccg cgatagagcc gtccgcccaa aatgaggccg ccgcccacgc cggtccccag
      781 agtgaaagcc actaaatttt ggacgcgccg ccggaattcc aaatggtagg cgccccaggc
      841 cgcggcgttg gcgtcgttct ccacaaaaag cggccggggg aaccggtgct ttttgaagag
      901 ggcggccagg gggacgttgt tccaacggag gttcggggca aaacggacga cccccttgac
      961 cgggtccacg tcgccggcga cgccgacgcc cgtgccccgc aggggacggc gcagccacgg
     1021 cgtcacggcc tttttcaagg ccgcgaccaa agccgtcgga cggcggtcgg tatcgacccg
     1081 ttgaagttcc cgaatccctc cccgctcgtc gaccagggcc aatttgacct gggtgccgcc
     1141 caagtcgatg cccagcctcc atccgtttcg catggtgtct ctcttacgcc gcttt
//
LOCUS       DAOQLS010000764         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_106005_length_1193_cov_4.323374, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000764 DAOQLS010000000
VERSION     DAOQLS010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106005_length_1193_cov_4.323374"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK362_11325"
     CDS             <1..252
                     /locus_tag="PK362_11325"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNC75458.1"
                     /translation="AEWKGCALIFFSAANESPPAGTELALAAPVLTVSDRAGFARDGG
                     MIGFVTAGSRLRLELNGPAADRAGLKISAKLRQLATEVQ"
     gene            249..>1193
                     /locus_tag="PK362_11330"
     CDS             249..>1193
                     /locus_tag="PK362_11330"
                     /inference="COORDINATES: protein motif:HMM:NF028461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HNC75459.1"
                     /translation="MKARNLAGLPIRLSLLLYGGAVGAAALLLACGAFVLYDRLTYPK
                     VLARELEEIASIVGDNSSAALAFQDTQNAAEILRTVRANRDIVAGAVYTESGSVFTAY
                     HRDPNIAPDPGPVQPAGLYRTGTSLRLYRPIEVEGDIVGTVFLEMTLGELDRRLHRYA
                     LLSLAVLAVVMVLCLGLARWLQGFIGDPLRRAAEVVETIVHQRDLTLRLEDPGNSEIG
                     QLVRSFNHMVNSLGSVIRKVSGITAALNQSAAGLQGASGQMGRVAKDSASQASLVSTA
                     AVEVSHNVQTVAENIVRLAAGLEDTLRQARQGHEAAEHS"
BASE COUNT          204 a          412 c          366 g          211 t
ORIGIN      
        1 gcggaatgga agggatgcgc cctgattttt ttcagcgccg ccaacgaaag cccccccgcc
       61 gggacggaac tcgcgctcgc cgcccccgtg ctcacggtgt cggaccgggc cgggtttgcc
      121 cgcgacggcg gcatgatcgg atttgtcacc gcgggctcgc gcctccgcct ggagctgaac
      181 ggaccggcgg ccgatcgggc cggccttaaa atcagcgcaa aacttcgcca actcgccacg
      241 gaggtccaat gaaggcgcgc aatttggccg gcctgcccat ccgcctcagc ctcttgctct
      301 acgggggggc cgtgggcgcc gccgccctcc ttctggcctg cggggcgttc gttctttacg
      361 accgcctcac ttaccccaaa gtgctggcgc gggaattgga ggaaatcgcc tccatcgtcg
      421 gcgacaattc ttccgccgcc ctggcgtttc aggacaccca gaacgcggcg gaaattctcc
      481 gaacggtacg ggccaacaga gacatcgttg ccggggcggt gtacaccgaa agcggctccg
      541 ttttcaccgc ctaccaccgc gatcccaata tcgccccgga tccgggaccc gttcaacccg
      601 cgggccttta ccgaaccgga acgtcccttc gcctctaccg acccattgag gtggaaggcg
      661 atatcgtggg caccgttttc ctggagatga cgttgggcga attggatcga cgcctgcatc
      721 gatacgccct gctgtccctc gcggttttgg ccgtcgtgat ggtcctctgc ctggggttgg
      781 cgcggtggct tcaagggttc atcggcgatc ccctccgacg ggcggccgag gtcgttgaaa
      841 ccatcgtcca ccagcgggat ctcaccctcc gactcgaaga tcccgggaac agcgagatcg
      901 gccaattggt ccgctcgttc aatcacatgg tcaacagctt ggggagcgtg atccgaaaag
      961 tcagcggcat caccgccgcc ttaaaccagt cggccgccgg ccttcagggc gcctccggcc
     1021 aaatgggccg ggtggccaag gattcggctt cccaggcttc cctcgtgtcc accgccgcgg
     1081 tggaagtgag ccacaacgtc caaaccgtcg cggaaaacat cgttcgtctg gcggccgggc
     1141 tcgaggacac cctccgccag gcccggcaag ggcacgaagc cgcggagcat tcc
//
LOCUS       DAOQLS010000765         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_106401_length_1191_cov_2.687500, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000765 DAOQLS010000000
VERSION     DAOQLS010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106401_length_1191_cov_2.687500"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PK362_11335"
     CDS             complement(<1..301)
                     /locus_tag="PK362_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75460.1"
                     /translation="MTEKKKAVETAEPAVNPDAPLSQLLLDCTKEKYRLVSLATRWAQ
                     EVKVRDQSTLQPQELLDVALKEIMLKKVTLEAIEKLPPPPKVEKKDIEFTLPTLSL"
     gene            complement(318..950)
                     /gene="gmk"
                     /locus_tag="PK362_11340"
     CDS             complement(318..950)
                     /gene="gmk"
                     /locus_tag="PK362_11340"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNC75461.1"
                     /translation="MIRGLLLVVSAPSGAGKSTLCQALVQRRPDLTLSVSCTTRPPRP
                     TEKNGVDYHFLSETDFEKRVTEGAFLEWARVHGFAYGTPRDRIDQALAAGRDVVLNID
                     IQGAESVRKAFPLESVRVFITPPSWKELEARLRGRNQDSDDVIQRRLANARAELAHAS
                     RYDYWVINDDLENALEELDVILRAEHRRVPRLSEELAALEHWGLAKSARI"
BASE COUNT          191 a          335 c          413 g          252 t
ORIGIN      
        1 tgagcgagag cgtggggagg gtgaactcga tgtccttctt ttccaccttc ggcggcggcg
       61 gaagcttttc gatggcctcg agcgtcactt ttttgagcat gatttccttc agggcgacgt
      121 ccagcagttc ctggggctgc agggtggact ggtcccggac tttcacttcc tgggcccaac
      181 gggtggccag ggagaccagg cggtattttt ccttggtgca atccaacaag agctggctga
      241 ggggcgcgtc cgggttgacg gcgggttcgg cggtttcgac ggcttttttc ttttcggtca
      301 cggtgttcct cctggaatca aatccgggcg ctcttggcga ggccccaatg ctccaacgcg
      361 gcgagttctt ccgacagacg cggcacgcgg cggtgttcgg cccggaggat cacgtccagt
      421 tcctccagag cgttttccaa atcgtcgttg atgacccaat aatcgtagcg ggacgcgtgg
      481 gccagctccg cccgggcgtt ggccaggcgg cgctgaatga cgtcgtccga atcctggttg
      541 cggccccgaa ggcgcgcttc cagttctttc caggaaggcg gcgtgatgaa gacgcgcacg
      601 ctttccaggg ggaaggcttt ccgcacggat tccgcgccct ggatatcgat gttgagcacc
      661 acgtcccggc ccgcggccag ggcctggtcg atccggtccc ggggggtgcc gtaggcgaac
      721 ccgtgcaccc gggcccattc caaaaaggcc ccttcggtca cgcgcttttc aaagtcggtt
      781 tcggacaaaa agtggtagtc caccccgttt ttttccgtcg gacgcggggg ccgggtggtg
      841 caggagacgg acaacgtcag gtccgggcgg cgttgcacca gggcctggca aagagtggat
      901 ttcccggcgc ccgagggcgc cgacacgaca aggagaaggc ctcggatcat ggggatttgg
      961 gggaccgttg gttattatag cttttccatc cgccgacggt caatcggcgt tcccggccct
     1021 tcttattttc cgggggagaa ggattcaaag tcggccggcg ggggggcttc gaacatcagg
     1081 gattttttgg tgcgggggtg gaaaaaagtc aattttcggg cgtgcaggag ggggcgcgga
     1141 ccctcttcgg cggcgtcgta agtcgtgtcg ccgaccaggg ggtggcccag g
//
LOCUS       DAOQLS010000766         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_106675_length_1189_cov_4.716931, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000766 DAOQLS010000000
VERSION     DAOQLS010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106675_length_1189_cov_4.716931"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK362_11345"
     CDS             <1..396
                     /locus_tag="PK362_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003593089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HNC75462.1"
                     /translation="LSAVGPVWKVEEKDMDAVTALWGSGPAYVFYLAEAMAAAGAALG
                     LDPALAESLARQTVYGAGRLLAESADPAGELRRKVTSPGGTTQAALAVLEAAGVIPIF
                     EKALDAARRRSSELSSSARGSQAPKESNP"
     gene            393..656
                     /locus_tag="PK362_11350"
     CDS             393..656
                     /locus_tag="PK362_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNC75463.1"
                     /translation="MIIRVRVIPNAVRSEVVGRIGSTVRVKVAAPAIDGKANNELTTF
                     LAEFFEVKNRGVKIVRGQNGKEKTVEISGRAEEELEEVMETIP"
     gene            713..>1189
                     /locus_tag="PK362_11355"
     CDS             713..>1189
                     /locus_tag="PK362_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNC75464.1"
                     /translation="MPLSPYRWSGRCLWVLEQRDLPHRQRFIPCRSAADVARAIRDMS
                     VRGAPAIGNTAAFGLALAARERVFRRPGDLVAHLEKARARLAATRPTAVNLFWALERM
                     ARVWRPAPMDNLPTRLEREARAIHAEDLASCRRIGAAGAGLLPRGAVVLTHCNAGAL"
BASE COUNT          187 a          422 c          404 g          176 t
ORIGIN      
        1 ttatcggccg tcggccccgt ttggaaggtc gaggagaagg acatggacgc cgtgacggcc
       61 ttgtggggct cgggccccgc gtacgtgttt tacctcgcgg aagcgatggc ggccgccggc
      121 gccgccctgg gattggaccc cgcgctggcc gaaagcctgg cccgccagac ggtgtacggc
      181 gcgggacgtc ttctggccga atcggcggac cccgcggggg aattgcgccg gaaggtcacg
      241 agccccggcg gcaccaccca agccgccctc gcggtgctcg aggcggcggg cgttataccc
      301 attttcgaaa aggccctgga cgcggcccga cggcgatcga gcgaattgtc gtcgtcggcc
      361 cgcggatccc aggccccaaa ggagagcaac ccatgatcat tcgtgtccga gtgataccca
      421 acgcggtacg cagcgaagtg gtcgggcgca tcggttccac cgtgcgcgtc aaggtggccg
      481 ccccggccat cgacggaaaa gccaacaacg agctgaccac cttcctcgcc gagttcttcg
      541 aagtgaaaaa ccgcggcgtg aaaatcgtcc gcgggcaaaa cgggaaggaa aaaaccgtgg
      601 aaatttccgg acgggccgag gaagagctcg aagaagtgat ggagacgatc ccttaagaag
      661 cgggtcttcc ccgccccggc gcgcgtcggc cggggcgggg ccgcgccgct ccatgcctct
      721 ctccccctac cgctggtcgg gtcgctgcct gtgggtcctg gaacagcggg accttccgca
      781 tcgacagcgt tttatcccct gccgttccgc cgccgacgtc gcccgggcca tccgcgacat
      841 gtccgtgcgg ggcgccccgg ccatcggcaa caccgccgcc ttcggcctcg ccctggccgc
      901 ccgggaacgc gtgttccgcc gacccgggga tctcgtcgcc cacctggaaa aagcgcgcgc
      961 ccggttggcc gccacccggc ccaccgccgt caatttgttt tgggccctgg aacgcatggc
     1021 gcgcgtgtgg cgccccgcgc cgatggacaa tctcccgacg cgtctcgaac gggaggctcg
     1081 ggcgatccac gcggaggact tggccagttg ccgccggatc ggcgcggccg gcgccggtct
     1141 cctgccccgg ggcgcggtcg tcctcaccca ttgcaacgcc ggggccctg
//
LOCUS       DAOQLS010000767         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_107069_length_1187_cov_3.481449, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000767 DAOQLS010000000
VERSION     DAOQLS010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107069_length_1187_cov_3.481449"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK362_11360"
     CDS             complement(<1..369)
                     /locus_tag="PK362_11360"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNC75465.1"
                     /translation="MKTWPALFLGHGSPMNAIEDSPFASAWTELGRALGKPRAVLCVS
                     AHWLTRGTYLTGNSRPRTIHDFGGFPPELYDIQYPAPGDPALAEKTAARLGLAVGAVT
                     EDWGLDHGAWSVLRRLFPAAD"
     gene            598..>1187
                     /locus_tag="PK362_11365"
     CDS             598..>1187
                     /locus_tag="PK362_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC75466.1"
                     /translation="MSFESLSLHPALVKATAALGYTEPTPIQAKAIPLALSGRDVLGA
                     AQTGGGKTAAFVLPILHGLLNKPAPGPRVLILVPTRELAVQVDRVIKECSRFSPVKTA
                     VIIGGVGHSGQTHAVRQGAQILVATPGRLMDHLEQKNFQLHRIDQLVLDEADRMLDMG
                     FLPDIKDILKHVPPERQTQLYSATLHKDVERIAIGHA"
BASE COUNT          229 a          419 c          330 g          209 t
ORIGIN      
        1 gtcggccgcc ggaaagaggc ggcgcaacac gctccaggcg ccgtggtcca gcccccaatc
       61 ctccgtcacg gcgccgacgg ccagccccaa ccgggcggcg gttttttcgg ccaaagccgg
      121 gtccccggga gcggggtatt gaatgtcgta caactccggt ggaaacccgc cgaaatcgtg
      181 aatcgttcgg ggccgggagt tccccgtcaa atacgttccc cgggtgagcc agtgggcgga
      241 cacgcacagg acggcccgcg gctttcccaa ggcgcgcccc aactccgtcc aggcggacgc
      301 gaagggcgag tcctcgatgg cgttcatcgg cgatccatga cccaaaaaca acgccggcca
      361 cgttttcatt cttatgtcct ggagagattt cgttccgcga tccatggctt ttatcaaaaa
      421 cgacgatcgg ggacaaaagt tccccctcgc ccccgccggg gccttccccc cgatttgaca
      481 ggggtccggc gatatgggac actgtcgtcg accgcagcgc cacgtccttc cggttttgtc
      541 tccaccgcaa cggcgaattc accataaaag gaacccacgg cttttttaag cctccccatg
      601 tcttttgaat cattgtccct gcaccccgcc ctcgtcaaag ccaccgccgc cctcggctac
      661 accgaaccca cccccattca agcgaaagcc attcctctgg ccctttcggg ccgggacgtt
      721 ctgggcgccg cccaaacggg cgggggcaag accgccgcct tcgttctgcc cattctccac
      781 ggactcctga acaaaccggc tccgggaccc cgggttctga tcttggtgcc gacgcgcgaa
      841 ttggccgtcc aggtggaccg cgtcatcaag gaatgctccc gtttttcgcc cgtcaaaacc
      901 gccgtcatca tcggtggcgt cggccacagc ggccagaccc acgcggtgcg ccagggggcc
      961 caaatcctgg tggccacccc cggccgcttg atggaccacc tggaacaaaa gaatttccaa
     1021 ctgcatcgca tcgatcagct ggtgctggac gaagccgacc gcatgttgga catgggcttt
     1081 ttgcccgaca tcaaggacat cctgaaacac gtccccccgg aacggcagac ccagctctat
     1141 tcggcgaccc tgcacaagga cgtggagcgc atcgcaatag gtcacgc
//
LOCUS       DAOQLS010000768         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_107453_length_1185_cov_2.986726, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000768 DAOQLS010000000
VERSION     DAOQLS010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107453_length_1185_cov_2.986726"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1185)
                     /locus_tag="PK362_11370"
     CDS             complement(<1..>1185)
                     /locus_tag="PK362_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75467.1"
                     /translation="SIELGASTAPVVVFVQDLHQNTDAQHNIGAAVRALSDARAVDLV
                     ALEGAFGPMDFGRLQSHPNRASVRAVADALLSLQKISGPVHAVWTLGPGAPPVVGVDD
                     PAHHRANIEAYRQSVRAAEGVRRSLARAEAALAARKAAELSPVLGALDRARADREAGR
                     LSVGDYVERLAASAPDAVSPVVKNFLSALADERTLDFEAAEKERAAVLSTLASRLDDG
                     DKNRLIAWSLGFRAGKIRHAEYHEFLRNLCARRGVDWKRTPTFARYVNYVLRAGSLDG
                     AALEAGIDALERKAFEKNTTTPGEARWLEETRRLAWRRRLAEFALTPREWAEGNPLGR
                     GATETLPPCFESFYREADARDRDMADNLLRALAERSARTAVLVTGGYHAHTHREARVK
                     LA"
BASE COUNT          180 a          410 c          416 g          179 t
ORIGIN      
        1 gcgccagctt cacgcgggcc tcgcggtgcg tgtgcgcgtg gtagccgccg gtgaccagga
       61 ccgccgttcg ggccgagcgc tcggccaggg cgcgcaaaag gttgtccgcc atgtcccggt
      121 cccgggcgtc ggcctcccgg taaaacgact cgaaacacgg gggaagcgtt tccgtcgctc
      181 cccggcccag ggggttcccc tcggcccatt cacggggcgt gagggcaaac tcggccagcc
      241 ggcggcgcca ggccaaccgc cgggtttcct ccagccagcg ggcctcgccc ggcgtggtcg
      301 tgtttttttc aaaggctttc cgctccaggg cgtcgatccc ggcttccaac gccgcgccgt
      361 ccagggatcc cgcccgaaga acgtaattca cgtaacgcgc gaacgtcggc gtacgcttcc
      421 aatccacccc ccgccgggcg cacaggttcc ggaggaattc gtggtattcc gcgtggcgga
      481 tcttccccgc ccggaaaccc aggctccagg cgatcagccg gtttttatcg ccatcgtcca
      541 accgggaggc gagggtggaa agaacggcgg cccgttcctt ttcggccgct tcaaaatcga
      601 gggttcgttc gtcggccagg gcgctcaaaa agttcttgac caccggggac acggcgtccg
      661 gcgccgaagc ggccaaccgt tccacgtaat cccccaccga cagtcgaccg gcctcccggt
      721 ccgcccgggc ccggtccaag gccccgagga ccggcgacaa ttccgccgcc ttgcgggccg
      781 ccagcgccgc ttcggcccgg gccaggcttc ggcggacccc ctcggccgcg cggacggatt
      841 gccggtacgc ctcgatgttg gcgcggtggt gagcggggtc gtccaccccg acgacgggcg
      901 gggccccggg gcccagggtc cagaccgcgt gaacggggcc ggaaatcttt tggagggaca
      961 ggagcgcgtc ggcgacggcg cggacgctcg cgcggttcgg gtgggattgc agacgcccaa
     1021 aatccatggg accaaaagcc ccttccaggg ccaccaggtc cacggctcgg gcgtcggaga
     1081 gggcacggac ggcggcgccg atgttgtgct gggcgtcggt gttttgatgg aggtcttgaa
     1141 cgaaaacgac cacgggggcg gtggaggcgc cgagttcgat cgagc
//
LOCUS       DAOQLS010000769         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_107862_length_1183_cov_2.096631, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000769 DAOQLS010000000
VERSION     DAOQLS010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107862_length_1183_cov_2.096631"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..635)
                     /gene="rpsE"
                     /locus_tag="PK362_11375"
     CDS             complement(114..635)
                     /gene="rpsE"
                     /locus_tag="PK362_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNC75468.1"
                     /translation="MPKIDANSLNLKETVVTINRVAKVVKGGKRFSFSALVVVGDGAG
                     HVGAAIGKAKEVQAAIQKAVSQAKKAIVKIPIKNGTIPHEVTGVFGAGTVLLKPAVSG
                     TGVIAGGSVRAVVEAAGVRDILSKSLRSPNPFNVVYATLAGLKDLETREDSYRRRGKE
                     LPAATAAAAPVAA"
     gene            complement(647..1012)
                     /gene="rplR"
                     /locus_tag="PK362_11380"
     CDS             complement(647..1012)
                     /gene="rplR"
                     /locus_tag="PK362_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNC75469.1"
                     /translation="MSLKSPQERLAFRRLRARRKIFGTAEKPRLSVYRAQRHMYAQLV
                     DDAAGRTLAAASSVMADLKKSLKTGATVEAATKVGQVLAEKAKALNISRAVFDRGGRA
                     YHGRIKAVADGARAAGLNF"
BASE COUNT          199 a          402 c          330 g          252 t
ORIGIN      
        1 cccagaccct tcccctcgac cgacaatttt tcgccggtgt cgtgatccgg gggcgggttt
       61 cagtgcgttt aaacgaatca tggttaagtc ccttataaaa ttaattaatg aaactaagcg
      121 gcgacgggag cggcggccgc tgtcgcggcg ggaagttcct taccccgacg gcgataggaa
      181 tcctcgcgcg tctccaaatc tttcaatccc gccaaggtgg catagaccac gttgaacgga
      241 ttggggcttc gcagggactt gctcaaaatg tcccgcaccc cggcggcttc caccaccgcg
      301 cgcacgcttc ccccggcgat aacgccggtt cccgagacgg cgggtttcaa caacaccgta
      361 ccggcgccga aaacaccggt cacttcgtgg ggaatcgtcc cgttcttgat gggaatcttg
      421 acgatggctt tcttcgcctg ggacaccgct ttctgaatgg ccgcctggac ttctttggct
      481 ttgccgatgg ccgcgcccac atgccccgcc ccatctccca ccaccaccag ggcgctgaac
      541 gaaaagcgct ttccgccttt caccactttg gccacccggt tgatggtgac caccgtttct
      601 ttcaagttta aactgttggc gtcgatcttt ggcacatggg ctcctgttaa aagttcagac
      661 ccgccgcgcg ggctccgtcg gccacggcct tgatgcggcc gtgataggcg cgtccgcccc
      721 ggtcgaacac ggcgcggctg atgttgaggg ccttcgcctt ttcggcgaga acctggccga
      781 ctttcgtggc ggcctccaca gtggccccgg tcttcaggga cttcttcaaa tccgccatca
      841 cgctggaggc ggccgccagg gttcgccccg cggcatcgtc gaccagctgg gcatacatat
      901 gccgctgggc ccgatacacc gacaatcgcg gcttctccgc cgtaccgaaa atcttccgac
      961 gggcgcgcag acgacgaaac gccagccgtt cttggggact ctttaaactc atttcttggc
     1021 tcctcccgcg gcgcccgcgg caccggccgc ggctttcccg gccttgcggc gcacgtgttc
     1081 cgtcgtgtaa cggataccca ctggctcgtt gttggcccag tacacgcccg gccgacggaa
     1141 ccgccgaatt tcggcggcga cctggcccac tttttctttg tcc
//
LOCUS       DAOQLS010000770         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_107876_length_1183_cov_1.866135, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000770 DAOQLS010000000
VERSION     DAOQLS010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107876_length_1183_cov_1.866135"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PK362_11385"
     CDS             complement(<1..892)
                     /locus_tag="PK362_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75470.1"
                     /translation="MHTQYEKQFGFALDERLYVGLISDYNQIANGFSTPLPNNRQINY
                     IGGTELVDYFSVRSWLETLIYHETAHNYQLNAKDNPVSSSLHKVFGNGMVISPFIIMP
                     NIAVNSFLLEGNAVLNESWHGNGGRLYSGRFKVQTLLQVKAGYFTPERMHNHTLFFPY
                     GEHHYSMGGFFQYYLAEKYGLVKTNRFFKENSLDWYWPFLTNNAMWRTIGRDFEGAMG
                     DYVKSAKKDAQSVVEVQGKVLVRSKYFSSLNGDKDELYFLVNQEGVRAPELARFNLKS
                     KSLLLRRESYMEGKVVKVGGS"
BASE COUNT          297 a          305 c          290 g          291 t
ORIGIN      
        1 aagacccgcc cactttgacg actttcccct ccatgtagct ctctcggcgg agcaataacg
       61 atttcgattt caggttgaaa cgcgccagtt ccggggcgcg aacgccctct tggtttacca
      121 gaaaatacag ctcgtccttg tccccgttca acgaagagaa atattttgac cggaccaaaa
      181 ccttcccttg gacttccacc accgactggg cgtctttctt agcactcttg acgtaatctc
      241 ccatggcccc ttcgaaatca cgaccaatcg tgcgccacat ggcgttgttg gtcaagaagg
      301 gccaatacca gtccagggaa ttttccttaa aaaaccgatt ggttttaacc agcccgtatt
      361 tctcagccag ataatattga aaaaatccgc ccatggagta atggtgttcg ccgtagggaa
      421 aaaagagcgt gtggttgtgc atgcgttccg gcgtaaaata gccggccttc acctgaagga
      481 gtgtttgaac cttaaaccgt ccgctgtaga gccgcccacc gttcccatgc caggattcgt
      541 ttaagaccgc gttcccctcc aacaaaaaag aattgacggc gatgttgggc ataataatga
      601 aaggtgaaat caccatgcca ttcccgaaga ctttatgaag cgagctggaa acgggattat
      661 ccttggcgtt caactgatag ttgtgggcag tttcgtggta aatcagcgtc tccagccacg
      721 atcgaacgct gaaatagtcc accagttccg ttccaccaat gtaattgatc tgccggttgt
      781 tgggcaacgg cgtcgaaaac ccgttggcga tttggttgta atccgaaatg agccccacgt
      841 aaagccgctc gtccagagcg aagccgaact gcttttcata ctgcgtatgc aagaaccctt
      901 ccaacgaagc cgcccgttcg gcgaccggaa ggttcttttt tgtgtagata atttggacgt
      961 tgttctggtt attggtgaaa taatcttgat cgtgtgaaac agtggcatca ccgaacagcg
     1021 gagaggcggt cgcgagcaat aaaaaaaagg atgttttttt aatggagaat tccacttaat
     1081 aaagttccgc gccgccttgg tggcaggcgg cacagttgcc gtcggcctca acgccgccga
     1141 tgtccacggg atgtttaaat ttcaagcctt gggtggacac cat
//
LOCUS       DAOQLS010000771         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_108650_length_1178_cov_3.928762, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000771 DAOQLS010000000
VERSION     DAOQLS010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108650_length_1178_cov_3.928762"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1076
                     /locus_tag="PK362_11390"
     CDS             117..1076
                     /locus_tag="PK362_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HNC75471.1"
                     /translation="MNRSFKTFFVILGAWVFGGSLWAEPKLDTARIEQWTGLKGVWNE
                     AEDVFKITYPRTDLDVNVAGVKLSPAQGLTAWVAFTPMGNRVAVMGDLVLAEDQVNPV
                     MSAALDYGLEVTALHNHFLWDSPRVMFMHIGGMGDAEALAAAVGKVFEKLKETAGGKG
                     PLPEVPETAGAPLDGKALDAVFGATGETTDGVYKIVLEKTTRMGGHSLGKTMGVNTWA
                     AFAGTDAKARVDGDVAAYESELQGVLKNLRKAGLFVTATHNHMTGESPRVIFVHYWGV
                     GPAGDLAKGIRSALNDQEKSKETKKRRPLNPAIQKWYPKTRID"
BASE COUNT          253 a          302 c          419 g          204 t
ORIGIN      
        1 ccatgacggg gatcaggggg tgggatggca agggcttccg ggtgggatac aatggggcgg
       61 ccgacgcccc ggggggccgg acgcgacgat ttgatgtcgg acaaaaggag aattcagtga
      121 atagaagttt taaaaccttt tttgtgattt taggggcttg ggtattcggc ggttccctgt
      181 gggccgagcc caagctggac acggcgcgga tagaacaatg gacggggttg aaaggcgttt
      241 ggaacgaagc ggaggatgtt tttaaaatca cttacccccg gacggatttg gacgtgaacg
      301 tggccggggt gaaattgagc cccgcccagg gcttgacggc ttgggtggcc tttacgccca
      361 tgggaaaccg cgtggccgtg atgggcgact tggtgctggc cgaagaccag gtaaacccgg
      421 taatgagcgc ggcgttggac tacgggctgg aggtgacggc cctgcacaat catttcctgt
      481 gggactcgcc ccgggtgatg tttatgcaca tcggcggaat gggcgacgcc gaggccctgg
      541 ccgccgccgt ggggaaagtg ttcgaaaagc taaaggagac cgccggcggc aagggcccct
      601 tgcccgaggt gcccgagacg gccggagcgc ccttggacgg caaggccctg gacgccgtgt
      661 tcggcgcgac gggggaaacg acggacgggg tttataaaat cgtccttgaa aagacgacgc
      721 ggatgggcgg gcactccctg ggaaagacca tgggggtcaa tacctgggcg gccttcgcgg
      781 ggacggacgc caaggcccgg gtggacgggg acgtggcggc ctacgagtcc gaactccagg
      841 gcgtgctcaa gaatttgcgg aaggcgggcc tgttcgtcac agccacacac aaccacatga
      901 cgggggaatc gccccgggtg atctttgtgc actactgggg agtgggtccg gcgggagatt
      961 tggccaaagg gattcgttcg gccttgaacg accaggaaaa aagcaaagaa acaaagaagc
     1021 gtcggcccct caacccggcc atccaaaagt ggtatccgaa aacgcggatc gactgatcct
     1081 tttcccggga gcccatgatt caaattgacg aggtgcccgc cggggcttgg acccgatggg
     1141 caccgcgctt tataaagcac gtccgagaca tcgacgtt
//
LOCUS       DAOQLS010000772         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_108824_length_1177_cov_5.073084, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000772 DAOQLS010000000
VERSION     DAOQLS010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108824_length_1177_cov_5.073084"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1177
                     /locus_tag="PK362_11395"
     CDS             <1..>1177
                     /locus_tag="PK362_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNC75472.1"
                     /translation="AKEASRFARHLVQAKSAAEEEARLRQTAESLWTAERLKEHVKLK
                     LSGNPLLVVSNREPYMHTREGRNLKWIVPAGGLVTALDPMMRAAGGTWVAHGAGDADW
                     ETVDDKNRLRVPPDDPFYTLRRVAITKEEENGYYYGFANEGLWPLCHIAHTRPLFRSQ
                     DFAAYQAVNEKFAAAVLEEIDGIEKPCVLIQDYHFALLPKMIKAKRPDARVAIFWHIP
                     WPNPEAFGICPWQRELLAGMLGADLVSFHTQFHCNNFMETVDRVLECRIEWERFTIHR
                     ENHPTLVKPFPISVDFPEPVTLPPPDRAALLKELGIKARFLAVGVERMDYTKGVLERF
                     HGIERFLERFPEYQGEFTYVQIGAPSRTHIKRYHDFLAEVDSEAERVNWKFKKRDWRP
                     "
BASE COUNT          251 a          375 c          339 g          212 t
ORIGIN      
        1 gccaaagagg cctcccggtt cgcgcggcat ttggtccagg ccaaatccgc cgcggaagag
       61 gaggcccggt tgcgtcagac ggccgaatcc ctctggacgg cggaacgttt gaaggaacac
      121 gtgaagctca aactgagcgg caaccccctc ctggtcgtct cgaaccggga gccctacatg
      181 cacacccgcg aagggcgcaa tttaaaatgg atcgttccgg ccggcggctt ggtgacggcg
      241 ttggacccca tgatgcgcgc cgcgggcggc acctgggtgg cccacggggc cggcgacgcc
      301 gactgggaaa cggtggacga caaaaaccga ttgcgggtgc cccccgacga tcccttttac
      361 acccttcggc gcgtcgccat caccaaggag gaggaaaacg gctactacta cgggttcgcg
      421 aacgaaggtc tctggcccct ctgccacatc gcccacaccc gtcccttatt ccgatcccag
      481 gatttcgccg cctaccaggc cgtcaacgag aaattcgcgg ccgccgtttt ggaggaaatc
      541 gacgggattg aaaaaccttg cgtgttgatc caggactacc acttcgccct tcttccgaaa
      601 atgatcaaag ccaaacgccc ggacgcccgg gtggctattt tctggcacat tccctggccg
      661 aacccggaag ccttcggcat ttgcccctgg cagcgtgaac tgctcgcggg catgctgggg
      721 gccgatttgg tgagctttca cacccaattc cattgcaaca atttcatgga aaccgtcgac
      781 cgggtgctcg aatgccgaat cgaatgggag cgattcacca tccatcggga aaaccacccg
      841 accctggtca aaccgtttcc catcagcgtg gactttcccg aacccgtgac tctccccccc
      901 ccggaccgcg cggccctgct gaaggagctg ggaatcaaag cccgcttcct ggccgtcggc
      961 gtcgagcgta tggattacac caaaggcgtc ttggagcgtt ttcacggcat cgagcggttt
     1021 ttggaaaggt tccccgaata ccaaggcgaa ttcacctacg tccagatcgg cgcgcccagc
     1081 cgcacgcaca tcaaacggta tcacgatttc ctggcggagg tggattcgga ggcggaacgg
     1141 gtgaattgga aattcaaaaa aagggactgg cgcccca
//
LOCUS       DAOQLS010000773         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_109024_length_1176_cov_4.502230, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000773 DAOQLS010000000
VERSION     DAOQLS010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109024_length_1176_cov_4.502230"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..510)
                     /locus_tag="PK362_11400"
     CDS             complement(163..510)
                     /locus_tag="PK362_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HNC75473.1"
                     /translation="MHDHGKDTYYHPGDLAHFGDMAKGNKELWDKFMGYYGAVFAKGA
                     LSEREKALIALGVALAVQCPYCIDAYTQACLEKGSNEKEMTEAAHVACAIRGGASLAH
                     GVQMRNVVDRLSM"
     gene            complement(521..>1176)
                     /gene="tyrS"
                     /locus_tag="PK362_11405"
     CDS             complement(521..>1176)
                     /gene="tyrS"
                     /locus_tag="PK362_11405"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNC75474.1"
                     /translation="DSVAVQSDVEIGGTDQLFNLLRGRDLQADFGQPPQVVMTLPLLE
                     GTDGVKKMSKSYGNAIALNDAPEDMFGKVMSLSDAMMWKYLELLTDRDPVPLRALHPM
                     EAKKTLAGDLVTRYRGADAATKARAHFETVFSKKGNPDDIPAYRVPRSPMSLTALMVE
                     AGLAPSKNEARRLLAQGAVELAGERVTEEREITVHSPLLIKVGKRQFRTLTPPAGTS"
BASE COUNT          191 a          375 c          376 g          234 t
ORIGIN      
        1 gggttcgaga acaggttctt gccggcgccg taggtcaccg gcggcaggcc ggcggtgctc
       61 agtcgggcgg caaagttcgg caccgcgggt tccgacaggg cccggcgttg ctccgccggg
      121 gcggccaggg ggttcccgcg ggcggtcagg gttccggcca tgtcacatcg acagacgatc
      181 gacgacgttc cgcatctgga cgccgtgggc cagggaggcc ccgccccgaa tggcacaggc
      241 cacgtgggcc gcttcggtca tttctttctc gttcgatcct ttttcgaggc aggcctgggt
      301 gtaggcgtcg atgcaatagg ggcattgaac cgccagggcc acgcccaggg cgatcagcgc
      361 cttttcccgc tcgctcaggg cccccttggc gaacacggcg ccgtaatagc ccataaactt
      421 atcccacaac tctttgttgc ccttcgccat gtcgccgaaa tgggccaaat cgccgggatg
      481 gtaataggtg tctttgccgt gatcgtgcat gcaatccccc ttaagaggtt ccggcgggcg
      541 gcgtcagagt acggaattga cgtttgccca ctttgatcaa cagcggcgag tgcacggtga
      601 tttcccgctc ctcggtcacc cgctcccccg ccaattcgac ggccccctgg gccaacagcc
      661 gccgggcttc gtttttcgag ggcgccaaac ccgcttcgac catcaaggcc gtcaagctca
      721 tcggggaccg gggcacccgg tacgccggaa tgtcgtcggg gtttcccttt ttggagaaca
      781 ccgtctcaaa atgcgcgcgg gctttggtcg cggcgtccgc gccgcgatac cgggtcacca
      841 ggtccccggc cagggttttt ttggcttcca tggggtgcag ggcccggagc ggaacggggt
      901 cccggtccgt caggagttcc aggtatttcc acatcatcgc gtccgagaga gacatcacct
      961 tcccgaacat gtcctcgggg gcgtcgttca acgcgatggc gttcccgtag gacttggaca
     1021 tctttttgac gccgtcggtg ccctccagca aaggcagggt catcacgacc tggggcggtt
     1081 ggccgaagtc ggcttgaagg tcccgtccgc gcagcaaatt gaaaagttgg tccgtgccgc
     1141 cgatttccac atcggattgg accgccacgg aatcgt
//
LOCUS       DAOQLS010000774         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_109076_length_1176_cov_3.057984, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000774 DAOQLS010000000
VERSION     DAOQLS010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109076_length_1176_cov_3.057984"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PK362_11410"
     CDS             complement(<1..567)
                     /locus_tag="PK362_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75475.1"
                     /translation="MAKRGSWIFAVFLFPWYPAVSRVDAATLQVNYDDRVLGGSTAFG
                     SNLDVNDNRHPEKHLDGERLLDRGFEASANQVWSSTFIAGGAGTIVRDTTEHYGGKAS
                     LKAVVTTVSPTARVLVVVAGPWVQSGETYQFSLWAKGGAYTGRLGVSLIDGNTFGLLG
                     STWTFDLAGSTWTQYTGSLPVTVNASSAL"
     gene            complement(619..>1176)
                     /locus_tag="PK362_11415"
     CDS             complement(619..>1176)
                     /locus_tag="PK362_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75476.1"
                     /translation="VLIFDSPTRLRFNSEVRAYSRSAGRFLVQEEGGVVAYPYVLEGG
                     QLKIAFPGEGVVVFRRPAKPAAVKPAGGQEYLVQGSFCSYSGSSNYSGSYSSSRKAFF
                     DGRGRFATGGESSFNTTLYDGGTAGGASSNAGPGGRYRVQGNKVFLTLSDGTTGVATV
                     NMRQSDGSVSELMYEGTLYAKGLCE"
BASE COUNT          239 a          378 c          355 g          204 t
ORIGIN      
        1 caaagccgac gacgcgttca cggtgacggg cagactcccg gtgtattggg tccaggtgga
       61 accggcgaga tcgaacgtcc atgtcgaccc caggaggccg aaggtgtttc cgtcgatgag
      121 ggagacgccc aaacggccgg tgtaggcgcc gcccttggcc cagagggaaa attgataggt
      181 ttcgccggat tgaacccagg gccccgcgac gaccacgagc acccgggccg tgggggagac
      241 cgtcgtcacc accgccttca acgacgcctt tcccccgtaa tgctcggtcg tgtcccggac
      301 gatcgttccc gcgccgccgg cgatgaaggt ggaactccac acttgattgg cggacgcttc
      361 aaagccccgg tccaacagtc gctcgccgtc caggtgtttt tccgggtggc ggttgtcgtt
      421 gacgtccagg ttcgagccga aggcggtcga accgcctaaa acccgatcat cgtaattcac
      481 ttgaagggtg gccgcgtcga cccgggaaac ggcggggtac cacgggaaaa ggaaaacggc
      541 gaaaatccac gacccgcgct tggccatgga catactatac gactcctttg ggggaatggc
      601 ctcaaccggg aggggcgttt actcgcacaa ccccttggcg tagagggtgc cttcgtacat
      661 cagttccgac acggacccgt cggattggcg catgttcacc gtggcgacgc cggtggttcc
      721 gtcggacaga gtcaggaaca ccttgttgcc ctgaacccgg tagcggcccc ccgggcccgc
      781 gttgctcgac gccccccccg cggtgccgcc gtcgtagagc gtcgtgttga aggacgattc
      841 gcctccggtg gcaaatcgcc cccggccgtc aaaaaacgcc ttccgtgaac tggagtagct
      901 tccgctgtaa ttcgacgatc ccgaataact gcaaaagctt ccttgaacca ggtattcctg
      961 gccgcccgcc ggtttgacgg ccgccggttt ggccggccgt cgaaaaacca cgacgccctc
     1021 tcccggaaag gctattttca attgcccgcc ctccaaaaca taggggtaag ccaccacgcc
     1081 cccctcttcc tgaaccaaaa aacgcccggc ggaacgggag taggctcgaa cttcggaatt
     1141 gaaccgaagc cgggtgggcg agtcgaaaat caacac
//
LOCUS       DAOQLS010000775         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_109215_length_1175_cov_4.067857, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000775 DAOQLS010000000
VERSION     DAOQLS010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109215_length_1175_cov_4.067857"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PK362_11420"
     CDS             <1..371
                     /locus_tag="PK362_11420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC75477.1"
                     /translation="VVSVGDGVVISRGWNTALGNLIKIRHNGMYTTYYGHLSRYAPGL
                     AVGQHVKQGQFIGRVGSTGYSTGPHLHFQIMKNGSLVNFLTLKMPSVGRLPPGRMKAF
                     LERRNNVLPPLEDLRPAPAA"
     gene            complement(428..>1175)
                     /locus_tag="PK362_11425"
     CDS             complement(428..>1175)
                     /locus_tag="PK362_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC75478.1"
                     /translation="NSMSDAPAPSPEKKRAFDTYELLVTSTEEITPKNRHIRFDLQGG
                     KTLHFKAGQFAQIFVPHDGKIRRTSYSIASSPQHTDYFELCVTLVDGGVSSTFLHKLR
                     PGDKVQAMAPLGTFTLKDETRDMVFISTGSGVAPFRSMIQDQLAKGTRKNLYLIYGHR
                     YEPDLLYRAEWEATMKSHSNFKVLFTLSRDTAWKGERGYVQEKIEKFVPGVKEKNYFI
                     CGLNNMITAVNDRLLSLGVPKEQIHFERYD"
BASE COUNT          228 a          352 c          349 g          246 t
ORIGIN      
        1 ccgtggtcag cgtcggcgac ggggtggtga tctcgcgggg ttggaacacc gccctcggca
       61 acctgatcaa aatccggcac aacggaatgt acacgactta ctacggtcat ctgtcccgct
      121 acgccccggg cctcgccgtg ggccagcacg tgaagcaggg acagttcatc ggccgcgtgg
      181 gctccacggg ctactccacc gggccccatt tgcatttcca aatcatgaaa aacggatcct
      241 tggtcaattt cctgaccctg aagatgcctt ccgtcggtcg cttgcccccg gggcgcatga
      301 aagcctttct cgaacggcgg aacaacgttc ttcccccgct ggaagacctt cggccggccc
      361 ccgccgccta aaaaaaaccc ccggagccca cgccgcggcc ccgggggacg aaaatccgcc
      421 gaccgtctca gtcgtatcgc tcgaagtgga tttgctcctt ggggaccccc agggacagca
      481 accggtcgtt gaccgccgtg atcatgttgt tgagcccgca gatgaagtaa ttcttctcct
      541 tcacgccggg aacgaatttc tcgattttct cctgcacgta gccccgctcg cccttccacg
      601 ccgtgtcccg gctcaaggtg aacaacactt taaagttgga atgggatttc atggtcgcct
      661 cccattccgc gcgatacaaa agatcgggtt cgtagcggtg accgtaaatc agataaaggt
      721 ttttcctcgt ccccttggcc aactggtcct gaatcatgct tcgaaaaggc gccacgcccg
      781 atcccgtgga aatgaacacc atgtcccggg tttcgtcctt gagggtgaaa gttcccagag
      841 gcgccatggc ctgaactttg tccccggggc ggagtttgtg gagaaacgtc gaggaaaccc
      901 cgccgtcgac cagggtgacg caaagctcga aatagtccgt gtgctggggg ctggaggcga
      961 tggaatagct cgtccggcgg atcttgccgt cgtggggcac gaaaatctgg gcgaactggc
     1021 cggccttgaa gtgaagggtc ttccccccct ggaggtcgaa gcggatgtgc cggtttttgg
     1081 gcgtgatttc ctcggtggag gtcaccaaca attcgtaggt gtcaaaagcg cgtttttttt
     1141 cggggctggg ggcgggagcg tcggacatgg agttc
//
LOCUS       DAOQLS010000776         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_109223_length_1175_cov_3.854464, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000776 DAOQLS010000000
VERSION     DAOQLS010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109223_length_1175_cov_3.854464"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..683
                     /gene="hisB"
                     /locus_tag="PK362_11430"
     CDS             63..683
                     /gene="hisB"
                     /locus_tag="PK362_11430"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNC75479.1"
                     /translation="MKDRVSEKNRVTKETRVRLRLNLDGSGRYHVSTTLPFLDHMLEL
                     FAKHGNFDLTVQAQGDTHIDDHHLVEDIGLTLGDVLLEALGDKRGIVRYGQDFRPELK
                     GKALTTMDETLSYVALDLSGRPYFDFKVKFRLQNKPPISFELFDDFFQALAMNAKMNL
                     HIKLLQGRNNHHIAESIFKGFGRALSMAVRVDPRRGSAVPSTKGAL"
     gene            680..>1175
                     /locus_tag="PK362_11435"
     CDS             680..>1175
                     /locus_tag="PK362_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75480.1"
                     /translation="MKLLRGLLIGLLTAGALVARVRAASTTTVEEAFLQAWEATQKSD
                     PKTAVFEKKAPGRYHFKTTRFPFDGELKVLNVTVNEGFGDDADLPAVGKYGFAEVELV
                     GWSKDDLMRYAQSYALWTQTHTLYHDAKGNRWLPTVEYFKSVRNAAVPVRRSLWASLS
                     TYLNI"
BASE COUNT          232 a          366 c          352 g          225 t
ORIGIN      
        1 gccctcctgg cccaggccga ggggctggtc taccacgccg cctccctcaa agcgagagtg
       61 aaatgaaaga ccgcgtgtcg gaaaaaaacc gcgtcaccaa ggaaacccgg gtccgccttc
      121 gattgaattt ggacgggtcc ggccggtacc acgtgtccac gaccctgccg ttcctggacc
      181 acatgctgga gctcttcgcc aagcacggca attttgattt gaccgtccag gcccagggcg
      241 acacccacat cgacgaccac cacctggtcg aggacatcgg tttgaccctg ggggacgttt
      301 tgctcgaggc cctgggggac aagcggggca tcgtgcgcta cggccaggat tttcgccccg
      361 agctcaaggg caaggccctc acgaccatgg acgaaacctt gtcttacgtg gcgctggatt
      421 tgtccggccg cccttatttt gatttcaagg tgaagttccg ccttcaaaac aagccgccga
      481 tctccttcga gcttttcgac gatttcttcc aggccctggc catgaacgcg aaaatgaacc
      541 tgcacatcaa gcttcttcaa gggcggaaca accaccacat cgccgaatcc attttcaagg
      601 ggttcggccg ggccctctcc atggcggtgc gggtcgatcc gcgccggggt tccgccgtgc
      661 cgtcgaccaa aggcgccctg tgaaattgct tcggggcctg ttgatcgggt tgttgaccgc
      721 cggggccctg gtggcccggg tccgggccgc ctccacgacc acggtggaag aggcgttcct
      781 tcaagcctgg gaggcgaccc agaagagcga ccccaaaacc gcggtgttcg agaaaaaggc
      841 cccgggccgc taccatttca agaccacgcg gtttcccttt gacggggagc tcaaggtgtt
      901 gaacgtcacc gtgaacgagg ggtttgggga cgacgcggat ttgccggcgg tggggaaata
      961 cggattcgcc gaggtggaat tggtgggctg gtccaaagac gacctgatgc gctacgccca
     1021 aagctacgcc ctgtggaccc agacccacac gctctaccac gacgccaagg gaaaccgctg
     1081 gttgcccacg gtggaatatt ttaaatcggt ccggaacgcc gccgttcccg tccgacggtc
     1141 cctttgggcg agcctgtcga cttatttgaa tatct
//
LOCUS       DAOQLS010000777         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_109902_length_1172_cov_1.927484, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000777 DAOQLS010000000
VERSION     DAOQLS010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109902_length_1172_cov_1.927484"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            964..>1172
                     /locus_tag="PK362_11440"
     CDS             964..>1172
                     /locus_tag="PK362_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75481.1"
                     /translation="MAVNEGLGAHRFVFLDSTDPHRVEARRVMADLALHSGAPAGDVV
                     AEKGLRDLLRWNSHRAELWRDLAHL"
BASE COUNT          172 a          298 c          384 g          318 t
ORIGIN      
        1 ggctttgcgg tggtgggggt gtgcgtgtgg cgagaatccc accgtcaatt tgtcgaggga
       61 ttgatcgtga cggttgtttg cgcccaaacc ctctttcttc tgctccctaa attattttca
      121 atccccgatt tctttatatt ccctgggaac ccccaatacg cgtcgttttg ggcctgcgcc
      181 gcggcgttct tagcccttgg gcatggaatg gccattagag atgtttctga aggggaaccg
      241 tcgtctccct ccctattgac tcgagcgggg tggggggtgg tctttctttt cgcggtgggt
      301 gggatattcc tgcttcctgt tcgctcaggc ctttgggcgt tggttgtggg agtgccggtt
      361 tttggttaca ctcgattcgg ccgccgggga ctggcagggg tcgggttcat tttgatcctt
      421 ggttttttgg cgatcccccc ttctcggatg gcccactctt tgaaatttga ggaagcgaaa
      481 agttttaaac ggaccgacat ttggcgagcg tcgttggcgg gtattgcgga acgcccgttg
      541 ttgggatggg gaccggccca gtatgagaac ttgtattggc aaaaaggact tccccaaaac
      601 gaagaacccg ttcgatttga aatgaccacg gatcgggccc acaatgactt cctccaaata
      661 agcgccgaaa gcggtgttct tgggggattg tttgccctcc tggccatggg tggccttttt
      721 tattcgtcgc ccaagggaaa acggagggcg ggggtttgtg cggcctgggc cggcgccggt
      781 gccttcgccc tggtgaattc tcctctggcc cttcccgcgt gcggggctct gtcgggatgt
      841 ttggcggcga tggcgccccc cggtcggcgg ccacgatggg tgtcttttga ttcatcggtt
      901 tatcgacgcg cagtggcctg gggcggggcc atttcgattt tgctttgttt gggtgaagtt
      961 gccttggcgg tcaacgaagg attgggcgcc catcgctttg tttttcttga ctccacggat
     1021 ccgcaccgcg tggaggcgcg acgggtcatg gcggatctgg ctctccacag cggggccccg
     1081 gctggggacg tggtcgcgga gaaaggcctt cgggatttgc ttcgatggaa ttcccatcgt
     1141 gccgagcttt ggcgggacct ggcgcatttg ga
//
LOCUS       DAOQLS010000778         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_109942_length_1171_cov_5.605735, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000778 DAOQLS010000000
VERSION     DAOQLS010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109942_length_1171_cov_5.605735"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PK362_11445"
     CDS             <1..397
                     /locus_tag="PK362_11445"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNC75482.1"
                     /translation="VLRRDVPPAVRLPLVQTVGRRFSRAGWAALAVLALTGLHRVRYL
                     LFCSPRYLTATPYGRLLSLKLGLFATMLSLSLWHDFSLGPALIRLADRPASPEFKAAS
                     RRLAFWARVNVLLVVAIVVIAAALRLTKD"
     gene            402..>1171
                     /locus_tag="PK362_11450"
     CDS             402..>1171
                     /locus_tag="PK362_11450"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75483.1"
                     /translation="MNLFAPLLDPESVPHAVLVITAVAALGLALGQLKVRGLGLGIAG
                     VLFSGLLFGHLGVTLNEHVLEFTREFGLILFVYAIGLQVGPGFFSSLKKEGLPLNLLA
                     ASVVLGGVAMAGIAHRWGGLDLPATVGVLAGATTNTPSLGAAQQALRDIAGPESELVK
                     TPGMGYAVAYPFGVIGIILVMVVLRTVFRIRPEKELEAFAQAKKQNAPGLQAINLEVT
                     NVNLAGIPIGRMPGSPQSTVVISRVRHGGRVSIARPDMT"
BASE COUNT          157 a          400 c          367 g          247 t
ORIGIN      
        1 cgtccttcgt cgggacgtcc cccccgcggt gcgcctgccc ctggttcaaa cggtgggccg
       61 gcgtttttcc cgcgccggct gggccgctct ggcggtcctg gccctcacgg ggctccaccg
      121 ggttcgttat cttttgtttt gttccccccg gtatttgacg gcgacgccct acggccggct
      181 tttgtcgctc aaactggggt tgttcgcgac gatgctgtcg ttgagcctct ggcacgattt
      241 ttccctcggt ccggcgctga tccgcctggc cgaccgaccg gcgtccccgg aattcaaggc
      301 ggcctcccgc cgtctggctt tttgggcccg cgtcaatgtt ttgttggtgg tggccatcgt
      361 ggtcatcgcc gcggccctgc gactcacaaa ggattaacgc catgaacctc ttcgccccgt
      421 tgttggatcc cgaatccgtc ccccacgccg tcctggtcat caccgccgtc gcggccctgg
      481 gactcgccct ggggcaactg aaggtccgcg ggttgggtct gggcatcgcc ggggttcttt
      541 tcagcggact gctgttcggc catctgggcg tcaccttgaa cgagcacgtg ctggaattta
      601 cccgggagtt cggactcatc cttttcgtct acgccatcgg cctgcaggtc ggcccggggt
      661 tcttttcctc cctaaaaaaa gaaggtctcc ccttgaacct cctggccgcg tcggtggtcc
      721 tgggcggcgt ggccatggcg ggcatcgcgc accgctgggg cggcctggac cttccggcga
      781 cggtcggcgt cctggccggc gccaccacga acaccccgag cctgggcgcc gcgcaacagg
      841 ctctccggga catcgcgggc cccgaatcgg aactcgtcaa aacccccggg atgggctacg
      901 ccgtggccta cccgttcggg gtgatcggga tcatcctggt catggtcgtc cttcgcacgg
      961 tttttcgaat ccgtccggaa aaggaactcg aggctttcgc ccaagccaaa aaacaaaacg
     1021 cgcccggcct ccaggcgatc aatttggaag tgacgaacgt caatctggcg ggcattccca
     1081 tcgggcgcat gccggggtcg ccgcaatcga cggtggtcat ttcccgggtg cgccacgggg
     1141 ggcgcgtgtc catcgcccgg ccggacatga c
//
LOCUS       DAOQLS010000779         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_110003_length_1171_cov_3.115591, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000779 DAOQLS010000000
VERSION     DAOQLS010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110003_length_1171_cov_3.115591"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..539
                     /locus_tag="PK362_11455"
     CDS             87..539
                     /locus_tag="PK362_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC75484.1"
                     /translation="MRPRRGFTLIELMLVVAIIGLLAAIAIPKFANMVIKAKEASIKG
                     KLGTLRSALSIYYADNEGIYPPSDYYLGIALTTGSRYLDSIPEISIPTVPDHVSARNI
                     ITSEMGYDWPNGVPGYYSWSYDYINTPSYAISVVCTHTDSSGHTWSLW"
     gene            657..1118
                     /locus_tag="PK362_11460"
     CDS             657..1118
                     /locus_tag="PK362_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC75485.1"
                     /translation="MPASRRPRGFTLIELMLVVAIIGLLAAIALPKFAELVVKAREAS
                     VKGGLGAIRSAMSIYYADNEGQNPNYVVIGESLTTGGKYLNEIPVMRSPQGYHDSSKI
                     VNRAASPPPSVMDGSGGWFYLGFPNWYAEGPQSLVVACTHTDSKGSLWSTY"
BASE COUNT          250 a          374 c          311 g          236 t
ORIGIN      
        1 taaaatgaat gctgttcaat gttttatcgt gtttttgagc cattgaaacc ggcgcgattt
       61 gggcctatcc tcaagcgagg gagcccatgc gcccacgacg cggatttacc ctgatcgaac
      121 tcatgctggt ggtggccatc atcggcctcc tcgccgccat tgccattccc aaattcgcca
      181 acatggtgat caaggccaag gaggcgtcga tcaaaggcaa attgggcact cttcgaagcg
      241 ccctgtcgat ctattacgcc gacaacgagg gaatttaccc gccctccgac tattacctgg
      301 gaatcgccct caccaccggg agccgctacc tggattcaat tcccgaaatt tccattccca
      361 ccgtgccgga ccacgtgtcc gccaggaaca tcattaccag cgagatgggg tacgactggc
      421 ccaacggggt cccgggctac tactcgtggt cctacgacta cataaacacg ccctcttacg
      481 cgatttccgt ggtctgcacc cacaccgact cgtccggtca cacctggagc ctctggtaat
      541 agtcccccca acggaaccct cgctccgagg gaatacctga cggtaattgt ccgccgaccc
      601 attgaattcg ttcggtgggg ggcctaaact gaagggaagg atccaggagg cgtcccgtgc
      661 ccgcttcaag gcgaccgcgt ggtttcacgc tgatcgagct gatgctcgtc gtggccatca
      721 tcggcctcct ggccgccatc gccctgccca aattcgccga actggtggtg aaggcccggg
      781 aagcctccgt caagggcggg ttgggggcca ttcgcagcgc gatgtcgatt tattacgccg
      841 acaacgaagg gcaaaatcca aactacgtgg tgatcggaga aagcctgacg accggcggca
      901 aatacctcaa cgaaatcccc gtcatgcgga gtccccaggg ataccacgat tcttcgaaaa
      961 tagtcaaccg tgccgcttcg ccgcccccca gcgtgatgga cgggagcggg ggatggttct
     1021 atctgggatt tcccaattgg tacgccgaag gaccacaaag ccttgtcgtc gcctgcaccc
     1081 acaccgacag caaaggctcc ctctggagca cctattaggg atccatcctt ccgggcccgc
     1141 ttttaagccc ccttcacgac gacccgttgg g
//
LOCUS       DAOQLS010000780         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_110141_length_1170_cov_4.782063, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000780 DAOQLS010000000
VERSION     DAOQLS010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110141_length_1170_cov_4.782063"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PK362_11465"
     CDS             complement(<1..133)
                     /locus_tag="PK362_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1009 domain-containing protein"
                     /protein_id="HNC75486.1"
                     /translation="MTPLGLIAGNGQFPLLVAQEARRRGRSVVVAAIEGETDPALERL
                     "
     gene            complement(156..1001)
                     /gene="lpxA"
                     /locus_tag="PK362_11470"
     CDS             complement(156..1001)
                     /gene="lpxA"
                     /locus_tag="PK362_11470"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNC75487.1"
                     /translation="MSSAAGDPPSAGIHPTAVVHPSAKIGAGVKIDPYAVIGPDVELG
                     RGTWVGAHAVVEFATVGEECRLHPHSFVGTEPQDLKFKGEKTRARLGARTTVRECVTI
                     HRGTAAAGETVVGSNCLLMAYGHVAHDAILADGVIMANAATLAGHVEVGPGAFIGGLV
                     GVHQFARIGAGAMIGAGSMVPADVAPFCMVQGDRAKIVGLNVVGLRRRGTSRDALSAI
                     KAAYRTVFGMGLPLAEAIAQIEKTERPAEVQVFLEFLKKTSHRGLCRPAPKTADGEDA
                     TEGFF"
     gene            complement(998..1165)
                     /locus_tag="PK362_11475"
     CDS             complement(998..1165)
                     /locus_tag="PK362_11475"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75488.1"
                     /translation="MKFRKPVVPGDQLELRVEVLRGGRVGKCRGEAFVKNEIVTEAEF
                     MFAIVDKQEAK"
BASE COUNT          182 a          395 c          391 g          202 t
ORIGIN      
        1 ccagacgttc cagggccggg tcggtttccc cctcgatggc ggcgaccacc acggaacgac
       61 cccggcgccg ggcttcctgc gccaccagca gcgggaattg gccgttcccg gcgatgagtc
      121 cgaggggagt caggggagcg gggcggccgg gcggctcaaa agaacccttc cgtggcgtcc
      181 tcgccgtcgg ccgtcttggg ggcgggccgg cagaggcccc ggtgggaggt ttttttcagg
      241 aactccaaga agacctggac ctccgccggt cgttcggttt tttcaatctg ggcgatggcc
      301 tcggccaggg gcagccccat gccgaagacg gtccggtagg cggctttgat ggcggacagg
      361 gcgtcccggg aggttccgcg acgccgcaga cccaccacgt tcaaaccgac aattttggcc
      421 cggtccccct gaaccatgca gaagggggcg acgtccgccg gcaccatgga gccggcgccg
      481 atcatggccc cggccccgat gcgggcaaat tgatgaacgc ccaccaagcc tccgatgaag
      541 gcgccgggtc ccacttccac gtgcccggcc agggtggcgg cgttggccat gatgaccccg
      601 tcggccagga tcgcgtcgtg ggccacgtgg ccataggcca tcaaaagaca gttggacccg
      661 acgacggttt cgccggccgc cgccgttcct cggtggatcg tcacgcattc ccgcaccgtg
      721 gtgcgggcgc ccaggcgcgc ccgggttttt tcccctttaa atttgaggtc ctggggttcc
      781 gtgccgacga aggaatgggg gtgcaggcgg cactcctcgc ccacggtggc gaattccacg
      841 accgcgtggg cgcccaccca ggtcccccgc cccaattcca cgtcggggcc gatgacggcg
      901 taggggtcga ttttcacccc cgccccaatc ttcgcggagg ggtggacgac cgccgtgggg
      961 tgaatcccgg cggaaggggg atcgccggcc gcggagctca tttggcttcc tgtttatcaa
     1021 cgatcgcgaa catgaattcc gcttcggtca cgatttcgtt tttcacaaag gcttcgccgc
     1081 ggcatttgcc cacccggccg ccccgcagca cttcgacccg aagttcgagt tgatcgccgg
     1141 gcacgacggg tttccggaac ttcaccttgt
//
LOCUS       DAOQLS010000781         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_111264_length_1164_cov_3.787196, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000781 DAOQLS010000000
VERSION     DAOQLS010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111264_length_1164_cov_3.787196"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK362_11480"
     CDS             <1..544
                     /locus_tag="PK362_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HNC75489.1"
                     /translation="RSAKPRPEMADRVREGLRARLEERRRAAPPLDPGELAATAELVS
                     QLPAETAKKIMDDLTQRDPVVGRRIADALFTFEDLTRLEDRDVQKILPRLNGVDLKMA
                     LRKCPAEISEKVFKNMSDRMSAALKEDIQNTPPQKKDVVEEAQRKIAVFIRDLVQKGE
                     VTPQRAAVAKTDGAPAEELV"
     gene            545..1126
                     /locus_tag="PK362_11485"
     CDS             545..1126
                     /locus_tag="PK362_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNC75490.1"
                     /translation="MSPRVFKKPTVSTRPIRVQEPPPAAREATPPAVPPETLRLMEET
                     LRQRLSVEFEERIARDRQADEAARAEERRRWLMDQEPALVELVLTLVAKICRTESAER
                     SDMAAAWVREGLLRLGGAGPRVVSVHPRDAEAVRSVEEARRPGVTVEENPDREPGDAV
                     IDTPTTRFDARLSTRLEEVERPLRTLSEGGLDG"
BASE COUNT          232 a          378 c          393 g          161 t
ORIGIN      
        1 ccgttccgcc aaacctcgcc ccgagatggc cgaccgggtt cgggaggggc tgcgggcccg
       61 cctggaggag cgccgacggg ccgccccgcc gctggacccc ggagaactgg ccgccaccgc
      121 cgaactggtt tcgcagttgc cggcggaaac cgccaaaaaa atcatggacg atttgaccca
      181 acgggacccg gtggtcggcc gacggatcgc cgacgccctg ttcacctttg aggatttgac
      241 ccgcctggag gaccgggacg tgcaaaagat tcttccgcgg ttgaacggcg tcgatttgaa
      301 aatggccctt cggaaatgcc ccgccgaaat cagcgaaaaa gtcttcaaaa acatgtcgga
      361 ccggatgtcg gcggccctga aggaagacat tcaaaacacc cccccgcaga aaaaggacgt
      421 ggtggaggaa gcccagcgaa aaatcgcggt gttcatccgc gacctggtgc aaaagggcga
      481 agtgaccccg caacgggcgg ccgtcgccaa gaccgacggc gcccccgcgg aggaattggt
      541 ctaaatgtcg ccccgggttt tcaaaaaacc gaccgtttcc acccgcccga tccgggttca
      601 ggaacccccg cccgcggccc gggaagcgac cccgcccgcc gttccccccg agacccttcg
      661 acttatggag gagaccctgc gccaacggtt gagcgtcgaa ttcgaggaac gaatcgcgcg
      721 ggaccgccaa gcggacgagg cggcccgggc ggaggagcga cgccgctggt tgatggacca
      781 ggaaccggcc ctcgtggaat tggtgttgac cttggtggcc aagatctgtc gaaccgaatc
      841 ggcggagcga tccgacatgg ccgcggcctg ggtgcgggag gggcttctgc gtttgggggg
      901 cgccggcccc cgggtcgtgt cggtccaccc ccgggacgcc gaagccgtcc gaagcgtgga
      961 agaagcgcgc cgacccggcg tgaccgtgga ggaaaacccc gaccgggaac ccggcgacgc
     1021 cgtgatcgac acgccgacca cgcggttcga cgcccggctg tcgacgcgtt tggaggaagt
     1081 ggaacgcccc cttcggaccc tctcggaagg cggattggat gggtgacatc gactgggcga
     1141 aatacgtcga cgtcctgggc gacc
//
LOCUS       DAOQLS010000782         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_111827_length_1161_cov_4.495479, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000782 DAOQLS010000000
VERSION     DAOQLS010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111827_length_1161_cov_4.495479"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /gene="ccsA"
                     /locus_tag="PK362_11490"
     CDS             <1..683
                     /gene="ccsA"
                     /locus_tag="PK362_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNC75491.1"
                     /translation="LYKNGLGALGAAGIGFLTQIIAHHLATQGDTMEMMRAVLDSNFW
                     LATHVVTVTIGYSSTFLSGFLAMFYILRGLFSSKLTTEAGRTLARMVYGIVCFSAFFS
                     FVGTVLGGIWADQSWGRFWGWDPKENGALLIVLWNALILHLRWGGFIRQRGLMVAAVF
                     GNIVTALSWFGVNMLGIGLHSYGFMDKAFPWLAAFVGTQVLLMVLGLLPDRFWGSRPW
                     PTEPAALS"
     gene            complement(685..>1161)
                     /locus_tag="PK362_11495"
     CDS             complement(685..>1161)
                     /locus_tag="PK362_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75492.1"
                     /translation="QNHGLAWGAATVQSIFSASDRLEVFVRRHFKLDRFPPAVSRPAT
                     AAEAPGAAPAGKRWARLEFPWPRCRFDARPFFPDFAGHFHGDPGANLVVRDERIAYFL
                     TGEEDRRGREEVLFAQALISTVAHENGCHRPLASEAVRLIQEFVLEKPAAGGPKAY"
BASE COUNT          197 a          359 c          363 g          242 t
ORIGIN      
        1 ggctctataa aaacggtctg ggggccctgg gcgcggcggg catcggtttt ttaacccaaa
       61 tcatcgccca ccatttggcc acccagggcg acaccatgga aatgatgcgg gccgtcctgg
      121 attccaattt ctggctggcc acccacgtgg tcacggtgac catcggatac agctccacct
      181 tcctctcggg atttttggcg atgttctaca ttcttcgagg acttttcagt tccaaattga
      241 cgacggaagc cggccggact ctggcccgga tggtttacgg gattgtctgt ttcagcgcct
      301 tcttcagttt cgtcggaacg gttttggggg ggatttgggc cgaccaatcc tggggccgtt
      361 tttgggggtg ggatcccaag gaaaacggcg cgctcctgat cgtcctctgg aacgcgttga
      421 tccttcatct ccgctgggga gggtttatcc gccagcgggg actcatggtc gcggctgttt
      481 tcggaaacat cgtgacggcc ctgtcctggt tcggcgtcaa catgctggga atcggcctgc
      541 actcctacgg tttcatggac aaggcgtttc cctggttggc ggctttcgtc ggaacccagg
      601 ttcttctgat ggtcttgggc ctgcttccgg accgcttctg gggcagccgc ccctggccca
      661 cggaacccgc cgccctttcg tgatttagta ggccttcggc ccgcccgcgg cgggtttttc
      721 caaaacaaat tcctgaatca aacgtacggc ctcgctcgcc aggggtcggt gacaaccgtt
      781 ctcgtgggcc accgtggaaa tcaaggcctg ggcgaaaagc acttcctccc gcccccgccg
      841 gtcctcctcc cccgtcaaga aatacgcgat ccgttcgtcc cggaccacga ggttggctcc
      901 cgggtccccg tgaaaatggc cggcgaagtc gggaaaaaaa ggccgggcgt cgaaccggca
      961 tcggggccag gggaactcca ggcgggccca acgttttccc gcgggggccg cgcccggggc
     1021 ctcggccgcg gtcgcgggcc gggaaacggc gggggggaac cggtccagct taaaatgccg
     1081 tcggacaaaa acctccaggc ggtccgacgc cgaaaaaatg gactgaacgg tcgcggcccc
     1141 ccaggccaga ccgtggtttt g
//
LOCUS       DAOQLS010000783         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_112599_length_1157_cov_3.637931, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000783 DAOQLS010000000
VERSION     DAOQLS010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112599_length_1157_cov_3.637931"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1157
                     /locus_tag="PK362_11500"
     CDS             <1..>1157
                     /locus_tag="PK362_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75493.1"
                     /translation="VGAFYADNAGLNLDRTPFANDFGVDEFLQLCERKGWTPVLTVNV
                     ASGAAHAADWVEYCNGNSGTTWGAQRAANGHPAPYGVRYWEVGNELWNQYSPEPGAIF
                     SRPHYTQANTNNYVAQFLLFAAAMKAKDPTIQVGAVGGARPSDSYLTDIVDPNWDNDV
                     LSGVGAPLGFIASHYYAPGADGGAPSPSAVFQSLLAAPDYFEQEMVNLAALAPDSVER
                     VVTEYGVSLVSTATAADRALGQTWGAGLYLAGMNNAFIRQGIVFAAQHDLLNEYESLL
                     EHTTDASGARVAVARAAHLVLALYGEGARGGHIVPATAAGTFSAPAVGWMSARANVPV
                     LDAAATLNPDEKTLTLFAVNRSTAAAQSTALRLAGFPGWLRAKGSAAITASS"
BASE COUNT          190 a          376 c          388 g          203 t
ORIGIN      
        1 gtcggcgcct tctacgccga caacgccgga ttgaacctgg accgtacccc ttttgccaac
       61 gatttcggcg tggacgaatt cctccaactc tgcgagcgaa aaggatggac gccggtgctg
      121 acggtcaacg tggcctcggg cgcggcccac gccgccgatt gggtggagta ttgcaacggg
      181 aacagcggca cgacctgggg cgcccagcgg gcggccaacg gccacccggc cccctacggc
      241 gtccggtact gggaagtcgg gaacgaactg tggaaccaat attcgccgga accgggggcg
      301 atttttagcc gcccccacta cacccaggcc aacacgaaca attacgtcgc gcaatttctg
      361 cttttcgccg ccgccatgaa ggccaaggac ccgacgatcc aggtgggcgc cgtgggaggg
      421 gcgaggccgt ccgattccta tttaaccgat atcgtggacc ccaattggga caacgacgtc
      481 ctgtcggggg tcggggcccc gttgggtttt atcgccagtc attattacgc cccgggggcg
      541 gacggcggcg cgccatcgcc gtcggccgtt tttcaatccc tgttggccgc gccggattat
      601 ttcgagcagg aaatggtgaa tttggcggct ttggcgccgg actcggtgga acgggtcgtc
      661 acggaatacg gcgtgtccct cgtcagcacg gccaccgcgg cggaccgggc cctgggccag
      721 acctggggcg cggggcttta tttggccggc atgaacaacg cttttattcg ccaagggatc
      781 gtgttcgccg cccagcacga tttgttgaac gagtacgaat ccctgctgga acacaccacc
      841 gacgcctcgg gcgcccgggt cgccgtggcc cgggccgccc atttggtttt ggccctctac
      901 ggcgaagggg cccggggggg gcacatcgtt cccgcgaccg ccgccgggac gttttcggct
      961 cccgccgtgg ggtggatgtc ggcccgggcc aacgtgccgg tgttggacgc ggccgcgacc
     1021 ctgaaccccg atgagaaaac cctgacgctc ttcgccgtca accgaagcac cgcggccgcg
     1081 caatcgacgg ccctgcggtt ggccgggttc ccgggttggt tgcgggcgaa aggatcggcc
     1141 gcgatcacgg catcgtc
//
LOCUS       DAOQLS010000784         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_112641_length_1157_cov_2.839383, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000784 DAOQLS010000000
VERSION     DAOQLS010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112641_length_1157_cov_2.839383"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1157
                     /locus_tag="PK362_11505"
     CDS             <1..>1157
                     /locus_tag="PK362_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75494.1"
                     /translation="LGEKFERPLELDLDHPVDPAIVAHLVASAIEAENEFFQNVTGEL
                     QRALTELYRWHAGVRGVTLYLYVDIAHHQVLIFSPVRSTDKSPGELRPEIRAISFAAG
                     DPVREFTPTRPPANSILFSLARAGGLPSFGWRRLDPDREMAEWKVGVLGLALEMPLLL
                     LAATTAPSPVFHVGLGLFALAHVALEARSGRLGGRSRSAAWATALSQLPARVIALAPY
                     ALVPGSPDAAHALAAVLVHLGFDFSVFGRPHLPHDPRDRSFDRGLRFPYDEESLSHRF
                     GSFHLSARDRFIGRDGRPGTIYSISLLAFPDPNSEVGRRIDALAADFLRQPFAPAFFA
                     TPSAHRHLTLRPYSVSPAAPASDADWTRIAAAAAADVARTPPYLYLVRDVG"
BASE COUNT          138 a          424 c          363 g          232 t
ORIGIN      
        1 ccctgggcga gaaatttgag cggcctttgg aattggacct ggaccacccc gtggatccgg
       61 ccatcgtcgc ccatctcgtg gcgtcggcca tcgaggccga gaacgaattt ttccagaacg
      121 tgacggggga actccagcgg gccttgacgg aactctaccg ttggcacgcg ggcgtgcggg
      181 gcgtgaccct ttacctgtac gtcgacatcg cccaccatca ggtcttgatt ttttctccgg
      241 ttcgatcgac ggataaaagc ccgggcgagc ttcgaccgga aatccgcgcc atctccttcg
      301 ccgccggaga tcccgtgcgg gaatttacgc cgacccggcc gccggccaat tccatcctgt
      361 tctccctggc ccgggccggg gggctgccgt ccttcggctg gcggcggttg gaccccgacc
      421 gggaaatggc cgaatggaaa gtgggcgttc tggggttggc cctggaaatg cccctgttgc
      481 tgctcgcggc gacgacggcg ccgtcgccgg tgttccacgt cgggctcggc ctttttgcct
      541 tggcccacgt ggctctggag gcgcgctccg gacgcctggg cgggcgttcc cggtcggcgg
      601 cctgggccac ggctctgtcc caactgccgg cgcgggtcat cgccctggcc ccctacgccc
      661 tcgtgcccgg gtcgcccgac gcggcccacg ccctggcggc ggtgttggtg catctgggtt
      721 ttgatttctc cgtcttcggc cgcccccacc ttccccacga tccccgggac cgttcgttcg
      781 atcggggtct ccggtttccc tacgacgagg aatccctctc ccaccggttc ggcagtttcc
      841 acctatcggc ccgggaccgt ttcatcggcc gggacggccg gcccgggacg atttattcca
      901 tttcactttt ggcctttccc gatccgaatt ccgaggtggg ccgccggatc gacgccctgg
      961 ccgcggattt tctccgccaa cctttcgccc cggccttttt cgcgacgccg tcggcccacc
     1021 ggcacttgac cctacgcccc tattcggtgt cgcccgcggc cccggcctcg gacgccgact
     1081 ggacccggat cgccgccgcg gccgcggccg acgtggcccg aacgcctccg tacctctacc
     1141 tcgttcggga cgtgggc
//
LOCUS       DAOQLS010000785         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_112966_length_1155_cov_4.426364, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000785 DAOQLS010000000
VERSION     DAOQLS010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112966_length_1155_cov_4.426364"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1155
                     /locus_tag="PK362_11510"
     CDS             <1..>1155
                     /locus_tag="PK362_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75495.1"
                     /translation="LGVYVLSFMVGGPPQYSPDYQYGWYYENGRPVYGLDRPTRRGIS
                     ILDPRRPHDIVKMLNRWAAVPGVDFVGLDYIRPVFGGNELVEDFVREMPGVTPPARYA
                     HMTKVERMKWIAPGRYVAPLLKTKDPARWKLSDQWFWYRAHCSARVVRQIAQEYAGKT
                     PLWGFTLSWQKGWEHAQDPVMFRDAGLDIDAIMLYEATGEQFRNLVGHWGNYALRDQL
                     NLVVGDTYDWRLHQKTTNPAGPEDFYNRMMMAVTGFHKDKPVRGIFTHDLNRALSGPR
                     QLGPYTSREWFLAGGAAMTRVRELNGALPYDLTLTAPDDARAGATLSAVIAFGERPST
                     APVRVEFFSGPDLEVSPGAVDLSPRKSSATLVLRWRPNDHSVARGRRTFLA"
BASE COUNT          191 a          376 c          368 g          220 t
ORIGIN      
        1 ctgggcgtct acgtgttgtc ttttatggtg gggggcccgc cgcagtattc gcccgattac
       61 caatacggct ggtactacga gaacggccgc cccgtttacg ggttggaccg gccgactcgc
      121 cggggaattt cgattttgga tccgcgccgt ccccacgaca tcgtcaagat gttgaaccgc
      181 tgggcggccg tgccgggggt cgatttcgtc gggttggact acatccggcc cgtcttcggc
      241 ggaaacgagc tggtggagga tttcgttcga gagatgcccg gcgtcacgcc gccggcccgt
      301 tacgcccaca tgacgaaagt ggagcggatg aagtggatcg ccccgggccg ctacgtggcc
      361 ccgttgctta aaaccaagga cccggcccgc tggaaactgt cggatcaatg gttttggtac
      421 cgggcccatt gttccgcccg ggtcgtgcgc caaatcgccc aggaatacgc cggtaaaacg
      481 cccctctggg gcttcaccct ctcctggcaa aagggctggg agcacgccca ggaccccgtc
      541 atgttccggg acgcgggttt ggacatcgac gccatcatgc tctacgaggc gaccggcgag
      601 cagttccgta atctcgtcgg ccactggggc aattacgccc ttcgggatca attgaatctc
      661 gtggtgggcg acacctacga ttggcgtctt caccaaaaaa cgacgaatcc cgcgggcccc
      721 gaggattttt acaaccggat gatgatggcc gtgacggggt ttcataagga caaacccgtg
      781 cggggaatct tcacccacga tttgaaccgg gccctgagcg gcccccggca actgggcccc
      841 tacacgtccc gggaatggtt cctggcgggc ggggccgcca tgacccgggt gcgggaattg
      901 aacggcgccc ttccctacga tttgacgttg acggcgccgg acgacgcccg cgccggcgcc
      961 acgctttcgg cggtgatcgc cttcggcgag cgcccctcca cggccccggt tcgcgtggag
     1021 tttttttccg gcccggacct ggaagtctcc cccggcgccg tggacctttc cccccggaaa
     1081 tcctcggcga cgctggtcct tcgatggcga cccaacgacc actccgtggc gcgggggcga
     1141 cgcacctttc tggcg
//
LOCUS       DAOQLS010000786         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_113016_length_1155_cov_3.105455, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000786 DAOQLS010000000
VERSION     DAOQLS010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113016_length_1155_cov_3.105455"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK362_11515"
     CDS             complement(<1..634)
                     /locus_tag="PK362_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75496.1"
                     /translation="MSSAPSDLQPQKVFTARRRRAILSFRRKGDVVWFVTSLGLFVAG
                     FTALAYTLLQLRRPLVIARAPDPAVTPLAPDAAPHPVAPAALTLPVPPAVEEPVEVPA
                     SAVDVEAEPPVQAEIPAPSPVKKSRAERREAARTETPPAPRTRAYSLLDDKATTQVLF
                     DKNARVAATPITAVGESALRLDYDFQRGEWVQAFLNIKQDLGKYTRVQFAF"
     gene            complement(631..>1155)
                     /locus_tag="PK362_11520"
     CDS             complement(631..>1155)
                     /locus_tag="PK362_11520"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC75497.1"
                     /translation="ISVGAVLLSLGVLENARGRPRRAEAHLDRARAIRENILGADSPA
                     VAESLGPWAEAVAELGRFETAEEAYLRAWGTFEKCFGPESPALLRTINNLAELYFRRS
                     ADEEAERLLRQAVTLAEKTHGPESPASTRALQNLLVAFEAQDRWDEAEAVRAKLTPTR
                     ARGRAAGHERTTRT"
BASE COUNT          188 a          375 c          403 g          189 t
ORIGIN      
        1 taaaagcgaa ttgcacccgg gtgtatttgc cgagatcctg cttgatgttc aggaaggcct
       61 gcacccattc accccgttga aaatcgtagt ccaaccgaag ggcggattct cccacggcgg
      121 taatcggcgt ggccgccacg cgggcgtttt tgtcaaaaag gacctgggtc gtcgctttgt
      181 cgtccagcag gctgtaagcg cgggtccgag gcgcgggagg cgtttcggtc cgggccgcct
      241 cccggcgttc ggcgcgggat ttcttgaccg gggacggagc gggaatttcg gcctggaccg
      301 gcggttcggc ttcgacgtcc accgcggagg ccgggacctc gaccggctcc tcgaccgccg
      361 ggggaaccgg aagcgtgagc gccgccgggg ccacggggtg gggggcggca tcgggcgcca
      421 aaggcgtcac ggcggggtcg ggcgcacggg cgatgaccag gggccgccgc agctggagca
      481 gcgtgtaggc cagggcggtg aacccggcga cgaaaagccc caggctggtg acaaaccaaa
      541 cgacgtcgcc tttccgtcgg aaagacaaaa tagcccgtcg ccgacgggcc gtgaaaactt
      601 tttgcggttg aagatcgctc ggggcggaac tcatgttcgt gtcgtcctct cgtggccggc
      661 ggcgcggccg cgggcccggg tcggggtcaa tttggcgcgc acggcttcgg cttcgtccca
      721 acggtcctgc gcctcgaagg cgacgaggag gttctggaga gcccgcgtcg acgccgggct
      781 ctccggtccg tgggttttct cggccagggt caccgcctgc cgaaggagcc gttcggcttc
      841 ctcgtcggcg gatcggcgga aatacagctc cgccaaattg ttgatcgtcc tcaggagagc
      901 cgggctctcc ggtccgaaac atttttcgaa ggtcccccac gcccgcagat acgcttcctc
      961 cgcggtttca aagcgcccca actcggccac cgcctcggcc caggggccca gggattcggc
     1021 caccgccggg gaatcggcgc ccaaaatatt ttcccggatg gcccgggccc ggtccaaatg
     1081 ggcctcggcg cggcggggac gcccccgggc gttttccaag acaccgagac tcaggagcac
     1141 cgcccccacc gaaat
//
LOCUS       DAOQLS010000787         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_113405_length_1153_cov_4.431694, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000787 DAOQLS010000000
VERSION     DAOQLS010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113405_length_1153_cov_4.431694"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK362_11525"
     CDS             <1..532
                     /locus_tag="PK362_11525"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNC75498.1"
                     /translation="GRPVIGICNGFQVLVKAGLLPGDDKPWDGSVQATLTDNDSGRFE
                     CRWIFLKTASRKSFWVKGLPEVFPLPVAHGEGKFVPASAALLNQWEKKGQVVFRYVDA
                     RGRKPAYPANPNGSAREVAGVTNAAGNILGLMPHPERNAFPFQHSHWTRVRPLPKTGV
                     GLQIFKNAVRYAAQVN"
     gene            542..>1153
                     /locus_tag="PK362_11530"
     CDS             542..>1153
                     /locus_tag="PK362_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNC75499.1"
                     /translation="MTHRYSEGVREGAAGWRWASALRDSWRAGYGGADFRADLLAGAV
                     VALVAVPLAMALAIASGVPPQYGLYTAVVAGTVVPLLGGSRFQVTGPTAAFVVVLAPV
                     AARFGLGGLLTAGFLAGLLLLVMGLGRLGGLIQYIPRTVTAGFTAGIGLVIAVLQLKD
                     FFGLQTGPLPDRFVDRLAVLAHAAPTLRPAELAVGFGTLVLLIL"
BASE COUNT          158 a          360 c          393 g          242 t
ORIGIN      
        1 gggccgtccc gtgatcggca tctgcaacgg gtttcaggtg ctggtcaaag ccggcctttt
       61 gcccggcgat gacaaaccct gggacggaag cgtccaggcc accttgaccg acaacgattc
      121 cggccgcttc gagtgccggt ggattttcct caaaaccgcg tcccgcaaaa gtttttgggt
      181 gaagggcctt ccggaagtgt tcccgttgcc cgtggcccac ggcgaaggca aattcgtgcc
      241 cgcgtcggcc gcccttttaa accaatggga aaagaagggc caagttgttt tccgctacgt
      301 ggatgcccgg ggtcgaaagc cggcctatcc ggccaacccc aacgggtccg cccgggaagt
      361 ggccggggtg accaacgccg ccggcaatat tttgggcctc atgccccatc cggagcgcaa
      421 cgcgttcccg ttccaacatt cccattggac ccgggtgcgg cctttgccca agacgggcgt
      481 ggggcttcag atttttaaaa acgccgtccg ctacgcggcc caggtcaatt aaacgttttc
      541 gatgacccac cgctactcgg aaggggttcg cgagggcgcg gcggggtggc gctgggcctc
      601 ggccttgcgg gattcctggc gggcggggta cggcggggcg gattttcggg cggaccttct
      661 ggcgggggcc gtggtggcct tggtggccgt ccccctggcc atggccttgg ccatcgccag
      721 cggtgttccg ccccaatacg gcctttacac ggcggtcgtc gcggggacgg tcgtgccact
      781 cctgggcggc tcccgctttc aagtaacggg ccccaccgcg gcgtttgtgg tcgtgttggc
      841 cccggtcgcg gcccgcttcg gactgggggg gctcctgacg gcggggttcc tggccggcct
      901 tctcttgttg gtcatggggc tcgggcgact ggggggattg attcaataca ttccccggac
      961 cgtcaccgcg gggttcaccg ccgggatcgg gttggtcatc gcggtccttc agttaaagga
     1021 ttttttcggt ttgcagacgg ggccgctgcc cgaccggttt gtcgatcggc tcgcggtgtt
     1081 ggcccacgcc gcaccgaccc tgcgcccggc ggagttggcc gtggggtttg gcaccttggt
     1141 tcttttaatt ctc
//
LOCUS       DAOQLS010000788         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_113520_length_1153_cov_1.849727, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000788 DAOQLS010000000
VERSION     DAOQLS010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113520_length_1153_cov_1.849727"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1153)
                     /locus_tag="PK362_11535"
     CDS             complement(<1..>1153)
                     /locus_tag="PK362_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75500.1"
                     /translation="SGDAPALRFETHTAPEGSSNFIQSARQGWEDFYPPERAENFNGS
                     KTPGEWLERWAADGGAWRHWMFRSAPTVATLIAGTAVAARGLAGAALTPVEIQLYPLV
                     AKITEWKAAFATAVSLSMVSLNLWAMGDRRSEHLGAALLATAFDFGTMLFLGASGLGL
                     ISLASRPTKGPAATTASTKAPKSLLRWAGLGILLLGLAWTARAAGMMEGSNPAANVSK
                     TIGADLAAPTPTRTGALPVLGAAVIPFRKARSRVATFPGRRSRSTREVDPEAGTPPVE
                     TTVRLRNEGAQWLAEEKASGRFYGVLADWIESSGPGSPHGSRHGTEPAGPFQEAAFNP
                     LTARALRFLAWQTDQALRTRDRGLRPTAEAIRMAYFWGLAGGTPSELPPL"
BASE COUNT          200 a          374 c          396 g          183 t
ORIGIN      
        1 agggggggaa gttccgatgg ggttcccccg gccaatcccc agaaataggc catgcgaatc
       61 gcttcggcgg tggggcgaag gccgcgatcc cgcgttcgaa gggcctgatc cgtttgccac
      121 gccaggaacc ggagggcccg ggccgtcaag ggattgaaag cggcctcctg gaacggaccg
      181 gccggttccg ttccgtgccg gcttccgtga ggacttccgg ggccggacga ttcgatccaa
      241 tcggccaaaa cgccgtaaaa gcgacccgag gctttttctt cggcgagcca ttgggcgcct
      301 tcgtttcgaa gccggacggt ggtttcaacc ggcggcgtcc cggcctcggg gtccacctcc
      361 cgggtggacc gggaacgcct cccggggaaa gtcgcgaccc gggaacgcgc cttccgaaac
      421 ggaataacgg ccgcgcccaa aaccgggagg gcgccggttc gggttggggt cggggccgca
      481 agatccgcgc cgatcgtttt cgaaacgttc gcggccgggt tggatccttc catcataccg
      541 gcggcgcggg ccgtccacgc gagtcccagc aggagaatcc cgagacccgc ccatcgcagc
      601 aaggatttgg gggctttggt tgacgcggtc gttgcggcgg gccccttcgt gggtcgggaa
      661 gcgagggaaa tcaacccgag gcccgaggcg cccagaaaca acattgttcc gaaatcaaag
      721 gccgtggcga gcaaggcggc gccgaggtgt tccgatcggc ggtcgcccat ggcccaaaga
      781 ttcaggctca ccatggaaag gctcaccgcg gtcgcgaacg cggccttcca ttcggtgatt
      841 ttggccacga ggggatacaa ttggatttca acgggggtca gcgccgcccc cgccagtccc
      901 cgggcggcca ccgccgtccc ggcgatcagg gtcgcgacgg tcggtgctga acggaacatc
      961 caatggcgcc aggcgccccc gtcggcggcc caccgctcca gccattcgcc gggcgttttc
     1021 gaaccgttga aattctccgc gcgttcgggc gggtaaaaat cctcccaccc ctgccgggcg
     1081 ctctgaatga aattggagct cccctcgggg gcggtgtggg tttcaaaacg aagcgccggg
     1141 gcgtcgcccg acc
//
LOCUS       DAOQLS010000789         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_113575_length_1152_cov_5.836828, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000789 DAOQLS010000000
VERSION     DAOQLS010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113575_length_1152_cov_5.836828"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1152)
                     /locus_tag="PK362_11540"
     CDS             complement(<1..>1152)
                     /locus_tag="PK362_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC75501.1"
                     /translation="PTAPVVPHIAPALAIAFVLTALAFVPLGQYVAERLRGFQARGET
                     LRGYFWELAGSLGGVVLFGLATAAGLPPAGGFLVLAGLSFFLRPPTRARWTVAVPVAL
                     ALFVAHRDRTEIHTPYYALRTHASPSGHGAFEVLANGALHQMALPVRRSDAPAASFLR
                     RARDGYQSPYDLLRRAPRRALVLGAGTGNDVAVLLDRGAESIDAVEIDPGILRLGERH
                     PDRPYASPRVRVINADGRAFLHRSTGTYDLIVLGTLDSMTRLSALSNVRLDNFIYTRE
                     SLAAIHRRLSPDGGLLMYFMISQPFIDGRLMDLLKNEFGRRPLARAEDDGVFNRIFAA
                     GPAFDHVGGALRDAPANAPVSIPPTDDWPYLYLRERGIPVHYGLAALQRA"
BASE COUNT          225 a          366 c          419 g          142 t
ORIGIN      
        1 ggcccgctgc agcgccgcca ggccgtaatg gacggggatc ccccgctccc gaagatacaa
       61 atagggccaa tcgtccgttg gcgggatcga gacgggggca ttcgccgggg cgtcccgaag
      121 agcgcccccc acgtggtcga aggccggtcc ggccgcgaag atccgattga agaccccgtc
      181 gtcctcggcc cgggccaggg ggcggcgccc gaattcgttt ttcagaaggt ccatcaagcg
      241 cccatcaatg aacggctggg agatcatgaa atacatgagg agtcccccgt cgggcgaaag
      301 ccgccggtga atcgccgcca acgattcccg ggtgtagatg aaattgtcca ggcgaacgtt
      361 ggacaacgcc gagagccgcg tcatggagtc gagcgtgccc aggacgatca ggtcgtaggt
      421 cccggtggac cggtggagaa acgcccgtcc gtcggcgttg atcacgcgaa cgcgcggcga
      481 cgcgtaaggg cggtcggggt gccgctcccc cagtcgaagg attcccggat cgatttccac
      541 ggcgtcgatg gattccgcgc cacggtccaa caggacggcg acgtcgttgc cggtcccggc
      601 gccgaggacc agggcgcgcc gcggcgcccg acgcaacagg tcgtagggcg attggtaacc
      661 gtcccgggcc cggcgcagaa aagacgccgc gggcgcgtcg gaccgccgga ccggcaaggc
      721 catttggtgg agagccccgt tggccagtac ctcaaaggcg ccgtggcccg agggcgaagc
      781 gtgggtccgc aaggcgtaat agggcgtgtg aatttcggtt cggtcccgat gggcgacgaa
      841 cagggccagg gccaccggca ccgccacggt ccagcgcgcc cgtgtgggag ggcgaaggaa
      901 aaaagaaagc cccgccagca ccagaaagcc tccggccggc ggcaggcccg ccgccgtggc
      961 gaggccaaag agaacgacgc ctccgagaga tcccgccaac tcccaaaaat agccgcgcag
     1021 ggtctcgccc cgggcctgaa accctcggag tcgttcggcc acgtactggc ccaggggaac
     1081 gaaggcgagg gcggttaaaa cgaaggcgat ggccagggcc ggcgcgatgt ggggaacgac
     1141 gggggcggtc gg
//
LOCUS       DAOQLS010000790         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_113798_length_1151_cov_3.978102, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000790 DAOQLS010000000
VERSION     DAOQLS010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113798_length_1151_cov_3.978102"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..>1151
                     /locus_tag="PK362_11545"
     CDS             67..>1151
                     /locus_tag="PK362_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNC75502.1"
                     /translation="MTDRPAPDLVTTKAVLPSGEDRRSYDDTLHCNRCGFCTSFCPTY
                     LATGDEALSPRARNQSFRALLENRLDPADAKRSFDTCLLCGICTSVCFAQVPTAKLMS
                     AAREKNREFLGAPLLARWVVRGLLPRPRLFETVLRWAYAPKRWGWSGLLARWGVLKAL
                     SPRLAAAEALVEEVPARFLREELSPAPADADVVQFIACGPNVVLPDVGRATGRLLAAA
                     GARAGAAPNVCCGLPGLSLGDREGARALARKNIEMLERYPRAIVLVDDSSCAATIKEY
                     PGLFPDDASWRERAEALRSRTRDLLEWLSRAGALSPETKGPVTYHDACKARQGQGLVQ
                     EPRRILTGAGVDLRELPEADQCCGGGGT"
BASE COUNT          166 a          384 c          401 g          200 t
ORIGIN      
        1 ccttgggacc gtcaagaaag ccttcgaccc ggacaatttg ctgaaccccg tgttgttcga
       61 acgcccatga ccgaccgccc cgcgcccgac ctggtcacga ccaaagcggt tctcccgagc
      121 ggggaggacc gccggtccta cgacgatacg ctccactgca accggtgcgg cttctgcacg
      181 tctttctgtc ccacgtacct cgccacgggc gatgaagcgc tgtcgccccg ggcgcgcaat
      241 caaagctttc gcgcgctgct ggaaaatcgt ttggaccccg ccgacgcgaa gcgatccttc
      301 gatacgtgcc ttttgtgcgg gatctgcacg tcggtgtgtt tcgcccaggt gcccacggcc
      361 aaactcatgt cggcggcccg ggagaaaaac cgggaatttc tgggggcgcc ccttctcgcg
      421 cggtgggtcg tccgaggcct cctgccgcgt ccccgtctct ttgaaaccgt cctgcgatgg
      481 gcctacgcgc cgaaacgatg ggggtggtcg gggcttctgg cccgttgggg ggtgttgaag
      541 gcgttgtccc cgcgtctggc cgccgccgaa gccctggtgg aggaagtccc cgcgcggttt
      601 cttcgggagg aactttcccc ggcgccggcg gacgccgacg tggttcagtt cattgcctgc
      661 ggtcccaacg tggtcctgcc cgacgtgggg cgcgcgacgg gccgccttct ggccgccgcg
      721 ggcgcccggg cgggagcggc gcccaacgtg tgttgcggcc tccccggcct gagtttgggg
      781 gaccgcgagg gggcccgggc cctggcccga aaaaatattg aaatgctgga gcgttacccc
      841 cgggcgatcg ttttggtcga tgattcctcc tgcgcggcga cgatcaagga gtatcccgga
      901 ctcttccccg acgacgcctc ttggcgggaa cgggccgagg ccctgcggtc gcggacccgg
      961 gacctgttgg aatggttgag ccgggcgggg gcgctctcgc cggaaacgaa gggccccgtg
     1021 acgtaccacg acgcctgcaa ggcccgccag ggccaggggc ttgttcagga accccgtcgg
     1081 atcttgaccg gagcgggcgt ggacctgcgg gaactcccgg aagcggacca atgctgcggc
     1141 gggggcggca c
//
LOCUS       DAOQLS010000791         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_113979_length_1150_cov_5.667580, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000791 DAOQLS010000000
VERSION     DAOQLS010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113979_length_1150_cov_5.667580"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /gene="ruvX"
                     /locus_tag="PK362_11550"
     CDS             complement(<1..326)
                     /gene="ruvX"
                     /locus_tag="PK362_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNC75503.1"
                     /translation="MTVCLGVDWGRARVGVAVSDESGLLAHPLETLPGGPPAAVAAAL
                     KTVAVDRGAGTVVVGLPLNMDGSEGESARAARALAAALESEGLRAILRDERLSTWRAD
                     ALLTEQG"
     gene            complement(323..877)
                     /gene="thpR"
                     /locus_tag="PK362_11555"
     CDS             complement(323..877)
                     /gene="thpR"
                     /locus_tag="PK362_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNC75504.1"
                     /translation="MKLFVSLAPPRELHTTLTRSFFPLREGVAGARWTKTEQFHFTLR
                     FIGPSDNVPALEVACREAARNAVPFDLELRGLGVFPTDGNPQVLWAGVGAGGDALNRL
                     AADLESRLGRAGFPPAEHPFQPHLTLARFTGAPRLPDGFLAAHADRPWFSFRADKIAV
                     MESQPTAYGVNHYTRSAFFLGAAG"
     gene            961..>1150
                     /locus_tag="PK362_11560"
     CDS             961..>1150
                     /locus_tag="PK362_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75505.1"
                     /translation="MTRRDVPWIGALAVAAALLFVPGFFLGRVPFAGDVTFSFHPWLT
                     YAAQEIQAGRLPLWNCYSS"
BASE COUNT          191 a          409 c          366 g          184 t
ORIGIN      
        1 ccctgttccg tcaaaagggc gtccgcccgc cacgtggaga gacgctcgtc ccggagaatc
       61 gcgcgcaacc cttcggattc cagggcggcg gccagggccc gggccgcccg ggcgctttcc
      121 ccttcggatc cgtccatgtt gagcggaagg cccaccacca cggtcccggc cccgcggtcc
      181 accgccacgg ttttaagggc cgcggccacg gcggccggcg gtccgcccgg caaggtctcc
      241 aggggatggg ccagaagacc cgactcgtcg gacacggcca cgcccacccg ggcccgaccc
      301 caatcgaccc ccaggcacac cgtcaccccg cggccccgag gaaaaaggcg ctccgcgtgt
      361 aatggttcac cccgtaggcc gtgggctggc tttccatcac cgcgattttg tcggcccgaa
      421 aggagaacca cggacggtcg gcgtgggccg cgaggaatcc gtccggaagc cgcggcgccc
      481 ccgtgaaacg cgccagggtg aggtggggtt gaaaggggtg ttccgccggc ggaaaaccgg
      541 cccgccccag gcggctctcc aagtccgccg cgagacggtt caaggcgtcg cccccggccc
      601 ccacgccggc ccaaaggacc tgggggtttc cgtccgttgg aaacactccc aagccgcgca
      661 attccagatc aaacgggacg gcgtttcggg ccgcctcccg gcaggcgact tccaaggcgg
      721 gcacgttgtc cgagggaccg atgaaccgaa gggtgaaatg gaattgctcc gttttggtcc
      781 accgcgctcc ggccacccct tcccgaagag ggaaaaaaga ccgggtcaac gtggtgtgaa
      841 gttcccgggg cggcgccaac gacacgaaca atttcatggg gccattgtcc caaagttgcc
      901 gaaaaatgaa aagggcggtt ctccttgcgg ggcccgcgcc cactgtccta cactgtcggc
      961 atgacccgtc gggacgtccc ctggatcggt gccctggccg tcgccgcggc cctgttgttc
     1021 gttcccggct tttttttggg ccgcgttccc ttcgccgggg acgtcacgtt ttccttccat
     1081 ccgtggctga cctacgccgc ccaggaaatt caggccggac gacttccgtt gtggaactgc
     1141 tattcctcct
//
LOCUS       DAOQLS010000792         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_114422_length_1148_cov_3.267155, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000792 DAOQLS010000000
VERSION     DAOQLS010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114422_length_1148_cov_3.267155"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK362_11565"
     CDS             complement(<1..385)
                     /locus_tag="PK362_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HNC75506.1"
                     /translation="MITPIAEPRGTANPTVLAWVDECARLTEPDRVLWCDGSEGEKRR
                     LIQECLADGELEELNQNLHPGAYLHRSATNDVARTEHLTFICTPTQDEAGPTNNWMAP
                     DEAYAKLRGIYRGCMRGRTMYVVPFI"
     gene            complement(387..>1148)
                     /locus_tag="PK362_11570"
     CDS             complement(387..>1148)
                     /locus_tag="PK362_11570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC75507.1"
                     /translation="GSPLGLLALRYYVLHRGLIHSWIAWELRVYGWHYIYAAIGTVLA
                     MAAWGLWAGRRLDDQRGRAELLERAAGRLRHLAATDGLTGVYVRRHLLEKLEEELRRA
                     ERYKTPIASLFVDVDNFKHFNETHGHIFGDEVLRQIARVIQKSVRESDVVGRYGGDEF
                     LVMLPHARAREAMLAAERVRQGVEALSIPKEGGPAVGVTVSVGVIAEIPHRADVLRFL
                     DIADQALRRSKGLGKNCTILCDTPASDTPHSPEET"
BASE COUNT          194 a          352 c          392 g          210 t
ORIGIN      
        1 tgatgaaagg caccacgtac atcgtccgcc ctcgcatgca tccccggtag atcccgcgca
       61 gcttcgcgta ggcttcgtcc ggggccatcc aattattggt cgggcccgct tcgtcctggg
      121 tcggcgtgca gatgaaggtc agatgttccg tgcgggccac gtcgttggtc gcggaccgat
      181 gcaggtaagc cccgggatgg aggttttgat tgagctcctc cagttcgccg tccgccaggc
      241 attcctggat caagcggcgt ttttcgcctt cggaaccgtc gcaccaaagg acccggtcgg
      301 gttcggtcag ccgggcgcat tcgtccaccc aggcgaggac ggtggggttg gcggtaccgc
      361 ggggttcggc gatgggggtg atcatattac gtttcctccg gagaatgtgg ggtgtcggaa
      421 gcgggggtgt cgcacaaaat ggtgcagttc ttgccgaggc ctttggaacg gcgaagggcc
      481 tggtcggcga tgtccagaaa acgcaaaacg tccgcccgat gggggatctc ggcgatgacc
      541 cccacgctca ccgtcacgcc caccgcgggc ccgccttcct tggggatcga cagggcttcc
      601 acgccttggc gcacgcgctc ggcggccagc atcgcctccc gggcccgggc gtgggggagc
      661 atgaccagaa attcgtcccc gccatagcgt cccaccacgt cggactcccg gacgcttttt
      721 tgaatgaccc gggcgatttg gcgcaggacc tcgtcgccga agatgtgccc gtgggtttca
      781 ttgaaatgtt tgaaattgtc cacgtcaacg aaaaggctgg cgatgggggt tttgtagcgt
      841 tcggcgcggc ggagctcctc ctccagtttt tcgagaaggt ggcgtcggac gtacacgccg
      901 gtcaatccgt cggtcgccgc cagatgacgc aagcggccgg cggcgcgctc caacaattcg
      961 gcgcggcccc gctggtcgtc caaacgccgg cccgcccaga gaccccaggc ggccatggcc
     1021 agcacggtgc cgatggccgc gtaaatgtag tgccagccgt aaacgcgcaa ttcccaggcg
     1081 atccaggagt ggatgaggcc gcgatggagg acgtagtagc ggagggccag gaggcccagg
     1141 ggggatcc
//
LOCUS       DAOQLS010000793         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_114872_length_1146_cov_2.438130, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000793 DAOQLS010000000
VERSION     DAOQLS010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114872_length_1146_cov_2.438130"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1146)
                     /locus_tag="PK362_11575"
     CDS             complement(<1..>1146)
                     /locus_tag="PK362_11575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC75508.1"
                     /translation="PIRPVPTDYPPLGAMAVLQSLEAAGHDPVFYDIDCFRPSLEEAV
                     AAIVAFKPEAIGVSAVVSTAYAHTQRLCLALKKALPDIPLVVGGNLAASAELLHRRCA
                     VDFCVVGEGERTSVALVDALARGPWRTDKERFRSIKGLTFLGADGQVVFTGYPDPLSP
                     EEFRDPDYTWLDRFSRIDNFIGDPFSRPDFARDPRSYAPHRKGMKLGTVTSAKGCVSR
                     CTFCHRWDKGYRPYGAEAVVRKIQYLKDRYNVGFFYFGDENFGSDRANLDELIEALKP
                     LDILYVVGGVRCRSVDPDLLRRMKESGCVALYYGMESGSPDILQVMEKVATLEHNRNA
                     ARWTREAGLYTVVQLVLGMPGECPRTIDETLAFVKEVTEPLPEPPHK"
BASE COUNT          206 a          351 c          398 g          191 t
ORIGIN      
        1 atttgtgggg cggctccggc aagggttcgg tgacttcctt gacgaaggcc agggtttcat
       61 cgatggtgcg ggggcattcg ccgggcatgc cgagcaccaa ctggaccacg gtgtagaggc
      121 cggcctcgcg ggtccagcgg gcggcgtttc ggttgtgctc cagcgtcgcc actttttcca
      181 tcacctgcag gatgtccggg ctgccggact ccatgccgta atagagcgcc acgcagccgc
      241 tctccttcat gcggcgcaga aggtccgggt ccacggagcg gcaccgaacg ccgccgacca
      301 cgtacaggat gtccaggggt ttcaaggcct cgatcaattc gtcgaggttc gcccggtcgg
      361 agccgaaatt ctcgtcgccg aaatagaaaa accccacgtt gtagcggtcc ttcagatatt
      421 ggattttccg gacgacggcc tcggccccgt aggggcggta gcctttgtcc caccggtggc
      481 aaaaggtgca acgggacaca cagcccttgg cggaggtcac cgtgcccagc ttcatgccct
      541 ttcggtgggg ggcgtaggag cgggggtccc gggcgaaatc cggtcgggaa aaggggtcgc
      601 cgatgaaatt gtcgatgcgg gaaaaccgat cgagccacgt gtaatccggg tcgcggaact
      661 cctcgggcga cagggggtcc gggtagccgg tgaacacgac ctggccgtcg gccccgagga
      721 aggtcaggcc cttgatggat cggaaacgtt ccttgtccgt ccgccagggc ccccgggcca
      781 gggcgtcgac cagggccacg ctcgttcgtt cgccttcgcc cacgacgcaa aaatccacgg
      841 cgcaacggcg gtgcagcaat tccgcgctgg cggccaggtt cccccccacg accaagggga
      901 tgtccggcag ggcctttttt aaagccaggc acagccgctg ggtgtgggcg tacgccgtgg
      961 aaacgacggc gctcacaccg atggcctcgg gcttgaaggc cacgatggcc gccacggctt
     1021 cctccaagga gggacggaaa caatcgatgt cgtagaacac ggggtcgtgg cccgccgcct
     1081 cgagcgactg gagaacggcc atggcgccga gcggggggta atccgtcgga acgggtcgaa
     1141 tggggg
//
LOCUS       DAOQLS010000794         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_115395_length_1143_cov_4.986213, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000794 DAOQLS010000000
VERSION     DAOQLS010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115395_length_1143_cov_4.986213"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PK362_11580"
     CDS             <1..640
                     /locus_tag="PK362_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC75509.1"
                     /translation="SGWLAHRMRNPLNVILMCAQYLKARFTDDDERREVVMAIEDKGQ
                     ALERMTHDFIRFSRTYEPRFRPENLGALLTESLAAAESDVRLHGVTVVKEWSETLPPV
                     PLDKELVGEVLGYLIDNAVEAMGGPGTLSLRAEVRGEDVVVQVGNTGGAIPAEARARM
                     FEPFFTTKERGTGLGLAISRRVVESHGGALTLLDGPDTTFEIRLPLRREGRP"
     gene            637..>1143
                     /locus_tag="PK362_11585"
     CDS             637..>1143
                     /locus_tag="PK362_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC75510.1"
                     /translation="MKKPAGPRVLVVDDEPSVGMIFHRILGDAGYEVISAANGAEGLR
                     ALKKQAPDLVFLDLQMPGMDGIETLRRLRETHPDLTVIIMTAYQTVNSAVETMKLGAL
                     DYLIKPLDAERLKSVVHQALELGEVTRRTPAVKGAGRPETLPIEEIVARGPEMSRVMS
                     LVEKVSPTD"
BASE COUNT          196 a          373 c          392 g          182 t
ORIGIN      
        1 gtccggctgg ctggcccacc ggatgcgcaa ccccctgaac gtcattttga tgtgcgccca
       61 atacctcaaa gcccggttca ccgacgacga cgagcgccgg gaggtggtca tggccatcga
      121 ggacaagggc caggctctgg agcgcatgac tcacgatttt atccgctttt cccgcaccta
      181 cgaaccccgc ttccggccgg agaacctggg ggcgctgttg accgagtccc tggcggcggc
      241 ggagtccgac gtccggcttc acggggtcac cgtggtcaag gagtggagcg aaaccctgcc
      301 gcccgtgccc ctggacaagg agttggtggg ggaagtcctg ggttatttga tcgacaacgc
      361 cgtcgaggcc atgggcggcc cgggcaccct gtcgcttcgg gccgaggtcc ggggcgagga
      421 cgtggtcgtc caggtgggca acacgggcgg ggccattccc gccgaggccc gggcgcggat
      481 gttcgaacct tttttcacca ccaaggaacg gggcaccggc ctggggctgg ccatttcccg
      541 acgggtggtg gaaagccacg gcggcgcctt gaccctgttg gacgggccgg acacgacctt
      601 tgagatccgc ctgcccctgc ggcgggaggg acgtccgtga aaaaaccggc gggcccccgg
      661 gtgctggtgg tggacgacga gccctcggtg gggatgatct ttcaccggat tctgggcgac
      721 gccggctacg aggtgatttc cgccgccaac ggcgccgagg gcctgcgggc cctcaaaaaa
      781 caggcgccgg acctggtctt cctcgacctt cagatgcccg gcatggacgg catcgaaacc
      841 ctgcggcgcc tgcgggagac ccatcccgat ttgacggtca tcatcatgac ggcctaccag
      901 accgtgaatt ccgccgtgga aaccatgaag ctgggggccc tggactattt gatcaaaccc
      961 ctggacgcgg agcgcctcaa atccgtcgtg caccaggccc tggagctggg cgaggtgacc
     1021 cgccggaccc cggcggtcaa aggcgccggc cgccccgaaa cccttcccat cgaggagatc
     1081 gtggcccggg ggcccgagat gagccgggtg atgtccctcg tcgaaaaagt gtcgcccacg
     1141 gac
//
LOCUS       DAOQLS010000795         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_116208_length_1140_cov_1.773272, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000795 DAOQLS010000000
VERSION     DAOQLS010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116208_length_1140_cov_1.773272"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1049)
                     /locus_tag="PK362_11590"
     CDS             complement(12..1049)
                     /locus_tag="PK362_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75511.1"
                     /translation="MTTLPTPDPILPDKIQLLMEEASCDRAEAELAMSLVGFDLEKAI
                     RTIGTLLRHIVVVKGKFVDTDQNRHGLLLLIADTRRNQFIRARAVVSYNPALYETSLS
                     QHWYDFERCIYAARLGEGALQQVSQDLERSFLDRLEAGRDAVISALKSPDPKELPSLL
                     NGGLSPSFPKGVQSVFVPELIDWDQFRRFPIRGEDRAPSPRPLVKTGAAAAGEPDGPA
                     PAHETGEDARPHGPGETLRIQMELVPDEAGRPVGEVRVGDRLFVRLADGRDIAHYVAK
                     LFGAVSENGPHPFEAPVETIQNEGERIRLFVRLSAGILGWADAPAAFRARVLPREADR
                     WWRRVWGRGPG"
     gene            complement(1046..>1140)
                     /locus_tag="PK362_11595"
     CDS             complement(1046..>1140)
                     /locus_tag="PK362_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018670814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNC75512.1"
                     /translation="GHKNLSTTQIYTHVTTERMRDVYEKAHPRA"
BASE COUNT          191 a          363 c          388 g          198 t
ORIGIN      
        1 cgggaggagc cttagccggg gccgcggccc cagacgcgcc gccaccagcg gtccgcttcc
       61 cgagggagaa cgcgggcgcg aaacgccgcg ggcgcgtcgg cccatcccaa aattccggcg
      121 gagagccgca cgaacaagcg gatccgttcg ccttcgtttt ggatggtttc caccggggcc
      181 tcgaaaggat gggggccgtt ttcgctcacg gcgccgaaaa gtttggccac gtaatgggcg
      241 atgtcccgcc cgtcggccag ccgcacgaac aaacgatccc ccacgcgcac ctcccccacg
      301 ggccggcccg cttcgtccgg gaccaattcc atttggatcc gaagggtttc gccggggcca
      361 tggggccggg cgtcctcccc ggtttcatgg gcgggcgcgg gtccgtccgg ctccccggcc
      421 gccgcggcgc cggttttcac cagcgggcgg ggactgggcg cgcggtcttc cccgcggatc
      481 ggaaaccggc ggaactggtc ccagtcgatg agttcgggga cgaaaaccga ttgcacgcct
      541 ttcggaaacg acggcgacag ccccccgttc aaaagagagg gaagctcttt ggggtccggg
      601 gatttgagcg cggaaataac ggcgtcccgc ccggcttcca accgatcgag gaaggaccgt
      661 tccaaatctt ggctgacctg ctggagcgcc ccctccccca gccgcgcggc gtaaatgcac
      721 cgctcgaaat cataccagtg ctggctcaag gaggtttcgt agagcgccgg gttatacgac
      781 accacggcgc gcgcccggat gaactggttg cggcgggtgt cggcgatgag gagcagaagc
      841 ccgtgccggt tttggtcggt gtcgacgaat ttgcccttga cgaccacgat gtgccgcagg
      901 agggtgccga tggtgcggat ggctttttcc aggtcgaagc ccaccagcga catggccaac
      961 tccgcttccg cccgatcgca agacgcctcc tccatcaaga gctggatttt gtcggggagg
     1021 atgggatcgg gggtcggcaa agtcgtcacg cgcgcgggtg ggccttttcg taaacgtcgc
     1081 gcattcgttc cgtcgtgacg tgggtgtaaa tctgcgtggt cgataggttt ttgtggccca
//
LOCUS       DAOQLS010000796         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_116803_length_1137_cov_2.124769, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000796 DAOQLS010000000
VERSION     DAOQLS010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116803_length_1137_cov_2.124769"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK362_11600"
     CDS             <1..268
                     /locus_tag="PK362_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75513.1"
                     /translation="FLAMEYIEGRDLRRLLRRHGWRAAETLYVGDALNDHRAATECGV
                     GFLGRLKAGARSPFPRGTPRAADFPTIARWIERFYSVPGGQESL"
     gene            265..1050
                     /gene="rfbF"
                     /locus_tag="PK362_11605"
     CDS             265..1050
                     /gene="rfbF"
                     /locus_tag="PK362_11605"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNC75514.1"
                     /translation="MKVAILCGGRGTRIRDVAENIPKPMVPIGPAPILWHIMKYYAHW
                     GHEEFVLCLGHKGDVIKDYFVNYQIRTSDVTVTLGSDKNVIYHTTNAENRWKVTLADT
                     GEDAMTGARIKRVQKYLAGEKDFLLTYGDGVGDVDLKKLVDYHRSHGKIMTLCGVHPT
                     GRFGEIISDNQGKVVEFHEKPKTGGGRVSGGFFVCRREIFDYLDDREDLVFEVEPMNR
                     LARDGQLMVYDHDGFWQPMDTYRDYNALNALYAQGKAPWMIWR"
BASE COUNT          221 a          370 c          356 g          190 t
ORIGIN      
        1 cttcctcgcg atggagtaca tcgagggccg cgacctgcgg cgcctgctcc gccgacacgg
       61 ctggcgcgcg gcggaaaccc tttacgtggg cgacgccttg aacgaccacc gcgccgcgac
      121 ggaatgcggc gtcgggttcc tgggccggct gaaggccggc gcccggtcgc ccttcccccg
      181 gggaacgccc cgggcggcgg attttccgac gatcgcccgg tggatcgagc ggttctattc
      241 tgtgcctgga ggtcaagaat ccctatgaaa gttgctatcc tatgcggggg ccggggaacg
      301 cgcattcgcg acgtggcgga gaacattccc aagccgatgg tccccatcgg ccccgcgccg
      361 atcctgtggc acatcatgaa atactacgcc cattgggggc acgaggagtt cgtcctgtgc
      421 ctcggccaca agggcgacgt gatcaaggat tatttcgtga actaccagat ccgcacgtcg
      481 gacgtcaccg tcaccctggg cagcgacaaa aacgtgatct accacaccac caacgccgag
      541 aaccgctgga aagtcaccct cgccgacacc ggcgaggacg ccatgaccgg cgcgcggatc
      601 aaacgcgttc aaaaatacct ggccggcgaa aaggactttc tgttgaccta cggcgacggc
      661 gtgggcgacg tggacttgaa aaagctcgtc gactaccacc ggtcccacgg caagatcatg
      721 accctctgcg gcgtccaccc caccggccgc ttcggcgaga tcatcagcga caaccaaggc
      781 aaggtcgtgg agttccacga aaagcccaaa accggcgggg gccgcgtgtc gggcggcttt
      841 ttcgtgtgcc gacgggaaat cttcgactat ctggacgacc gcgaggacct cgtgtttgaa
      901 gtggaaccca tgaaccggct ggcccgtgac ggccagttga tggtgtacga ccacgacggt
      961 ttctggcagc ccatggacac ctaccgcgac tacaacgccc tgaacgccct ctacgcccaa
     1021 ggcaaagcgc cgtggatgat ttggcgctga ccccggccgc tctcgtgccc ttgaagggcg
     1081 cccgggtcct cgtgaccggg gacacgggct ttaaggggtc ctggctgagt tattggc
//
LOCUS       DAOQLS010000797         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_116845_length_1137_cov_1.508318, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000797 DAOQLS010000000
VERSION     DAOQLS010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116845_length_1137_cov_1.508318"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PK362_11610"
     CDS             complement(<1..1047)
                     /locus_tag="PK362_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC75515.1"
                     /translation="MAGSPVVVAGAGLAGLSCAYHLKSPSILFDREPRPGGTARSVSV
                     GGFTFDFTGHLLHLHDPYTKTLIRRLLAGNWVECERKAWIHSHCVSTPYPFQANLHGL
                     PADVVRECVDGVREARRIFGVRPLEGAGPLNFKEWCGRLFGGGISRHFMIPYNEKLWG
                     VPATAMTPDWCGSFVPVPTLAEVEAGARRLHRKRFGYNTTFLYPRRGGIQALAEAFAE
                     KIPPLRLGESLAAVDWVHRRVMFASGESVPYRRLVSTLPLPELLKRLDPFPEELKDPL
                     NRLRWTTVLCVNVGVARPHISKASWIYFPEKKFPFYRVGFPMNFTPHVVPRGCSSMYV
                     EVSTRPGTEPSTDAG"
BASE COUNT          254 a          340 c          369 g          174 t
ORIGIN      
        1 gcccgcatcg gtcgagggtt cggtgccggg ccgggtggag acttccacat acatggacga
       61 acagccgcgc gggaccacgt ggggcgtaaa gttcatgggg aaccccaccc ggtagaaggg
      121 gaatttcttt tcggggaaat aaatccacga cgccttcgag atgtggggcc gcgccacgcc
      181 gacgttcacg cacaggacgg tggtccaccg aagacggttc aagggatctt tcaattcttc
      241 cggaaagggg tccaggcgtt tcagcaattc cggaagcggc aaggtggaga cgagccggcg
      301 gtagggcacg ctttcaccgg aggcgaacat cacccggcga tggacccaat ccaccgcggc
      361 gagggattcg cccaaccgca ggggcggaat tttttcggcg aaggcctccg ccaaggcttg
      421 gatcccgccc cggcgcggat agaggaaagt ggtgttgtag ccaaaccgtt ttcgatggag
      481 gcgccgggcc cccgcttcca cctcggccag ggtgggcacc ggcacgaatg aaccgcacca
      541 atccggcgtc atggccgtgg ccggaacgcc ccagagcttt tcgttgtagg ggatcatgaa
      601 gtggcgggaa atgccgcccc cgaacaaccg gccgcaccat tctttgaaat tcagcgggcc
      661 ggcgccttcc aacgggcgaa caccgaaaat ccgccgggct tcccgcacgc cgtccacgca
      721 ttctcggacc acgtccgccg ggagtccgtg gaggttcgct tgaaacgggt agggggtact
      781 gacgcagtgg gaatggatcc aggccttgcg ctcgcattcc acccaattgc cggccaaaag
      841 acggcggatc agcgtcttcg tgtaggggtc gtgcaaatgg aggaggtggc ccgtgaaatc
      901 gaacgtaaat ccgccgaccg acacggatcg ggcggttccg ccgggccgag gttcgcgatc
      961 aaataggatg gaaggagatt ttagatggta cgcgcaggaa agacccgcca gccccgctcc
     1021 cgccaccacc acgggggaac ccgccatcaa tgaaccacca cgtcgtgaag cttcaccatg
     1081 acaaaaagac cgaaaagcaa aattccggcg acgacggcca aggaaacgaa gaagggg
//
LOCUS       DAOQLS010000798         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_117585_length_1133_cov_2.510204, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000798 DAOQLS010000000
VERSION     DAOQLS010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117585_length_1133_cov_2.510204"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PK362_11615"
     CDS             <1..289
                     /locus_tag="PK362_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNC75516.1"
                     /translation="LMEYVEAHFREEEEQMLQASYPDFPHHKGQHLKLEAKSRELLEH
                     FGQGEEVLTVEISAFLSAWVNHHILKEDMEYIPWFQSRGLGQKPIPRARLL"
     gene            359..703
                     /locus_tag="PK362_11620"
     CDS             359..703
                     /locus_tag="PK362_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNC75517.1"
                     /translation="MNPIVLKTAGLLALSNLFMTFAWYGHLKGLTHKPWYIAVLVSWG
                     IAFFEYCLQVPANRLGATALNLSQLKILQEVITLSVFVPFAILFMGQPVRLNFLWAGF
                     CVLGAVYFIFRP"
     gene            700..>1133
                     /locus_tag="PK362_11625"
     CDS             700..>1133
                     /locus_tag="PK362_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC75518.1"
                     /translation="MTPHVLDHAFTLPLARSAVFPFFSNAENLELLTPRELHFKILSP
                     TPIPMKTGALIDYQIRLFGIPLKWRTEITLWNPPFQFIDTQLKGPYALWVHHHTFEER
                     GANATLLRDRVTYRLPLEPLGRLGLPLVRRELERIFRFRQEAV"
BASE COUNT          215 a          367 c          277 g          274 t
ORIGIN      
        1 cctcatggaa tacgtggaag cccatttccg ggaagaggag gaacaaatgc tccaggcctc
       61 ctaccccgac tttccccacc acaaaggcca acatttgaag ctcgaggcca aatcccggga
      121 gctgctggag catttcggcc agggcgagga agtactcacc gtggaaattt cggccttcct
      181 gtcggcctgg gtgaaccacc acattctcaa ggaagacatg gaatacatcc cctggtttca
      241 atcccgcggg ttgggtcaaa aacccatccc ccgggcccgc ctcctgtaat ctacgctata
      301 attccatcgg gcgccgtcgg cccccgcctt ttcgataatc ccgggcccag gacaaatcat
      361 gaatcccatc gttttaaaaa ccgccggtct tttggccctt tccaatcttt ttatgacctt
      421 cgcctggtac ggccacttga agggcttgac ccacaaaccc tggtacattg ccgttctggt
      481 cagttggggc atcgcttttt ttgaatattg cctgcaggtg ccggccaacc gtctgggggc
      541 cacggcgttg aacctttccc agcttaaaat tctccaggag gtcatcaccc tttccgtgtt
      601 cgtccccttc gcgatcctgt tcatgggcca gcccgtgcgc ttgaatttcc tctgggcggg
      661 tttctgcgtc ctgggcgcgg tctatttcat ctttcgtcca tgacccccca cgttttggac
      721 cacgctttca ctctgcccct ggcccggtcg gcggtgtttc cttttttctc caacgccgag
      781 aatttggaat tgctcacgcc ccgggagctt cattttaaaa ttctctcgcc gacgcccatc
      841 ccgatgaaaa ccggggccct catcgactat caaattcgcc ttttcggtat tccattgaaa
      901 tggcggaccg aaatcaccct ttggaatccg ccctttcaat tcatcgacac gcagttgaaa
      961 ggcccctacg ccctctgggt ccaccatcac acctttgagg agcggggagc gaacgccacg
     1021 ctcctgcggg accgggtgac ctatcgactg cccttggaac ccctggggcg gctgggcctg
     1081 cccctggtcc gccgagaact cgaaaggatt tttcggttcc gccaggaagc cgt
//
LOCUS       DAOQLS010000799         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_117673_length_1133_cov_1.519481, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000799 DAOQLS010000000
VERSION     DAOQLS010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117673_length_1133_cov_1.519481"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..>1133)
                     /locus_tag="PK362_11630"
     CDS             complement(181..>1133)
                     /locus_tag="PK362_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNC75519.1"
                     /translation="LAAESGADAAKFQNFRARHIVSDHGFRQLGTGKSHQSRWKKSVS
                     EVYQAASLPWEWTEALAKECRAAKIDYFSAPYDLEAVDMLDAHVDLYKIGSGDISWPE
                     MIVKVAGKGKPVLLATGASTLADVQRAVGLLRAARVPFSLMQCNTNYLGSVENFRHVH
                     LNVLNGYRALFPDAVPGLSDHTPGSVTVLGAVALGARTIEKHFTDDNSREGPDHPFSM
                     NPVSWREMVDRTRELESALGRPDKDVADNERDTVVIQRRCLRAARPLSAGRALGRADI
                     EVLRPAPLDAIFPYDLDKVLGRKPRRAIEAGDYFRWNDLD"
BASE COUNT          195 a          381 c          374 g          183 t
ORIGIN      
        1 ccgcggtcca agggccaggc gacgggccga acccccgcgg gcggaagcga cgccccgccc
       61 cgcgaccgaa gcgcgagggc gtgcaccgtc aggccggctt cggcaaaccg cgccagaaac
      121 cgccggtcgt gaggcgtgtc gcccggcccg acataaagca atcgctggag gggcaaggca
      181 tcaatccaaa tcgttccacc ggaagtagtc gcccgcctcg atcgcccggc ggggctttcg
      241 ccccaggacc ttgtccaaat cgtacgggaa aatggcgtcg agcggggcgg ggcgcaacac
      301 ctcgatgtcg gcgcgcccga gggcccggcc cgccgacagg ggccgggcgg cccgcaggca
      361 gcggcgttga atcaccacgg tgtcccgttc gttgtcggcc acgtctttgt ccgggcgccc
      421 cagggcggat tccaattccc gggtgcggtc gaccatttcc cgccaggaaa cggggttcat
      481 ggaaaacggg tggtccggcc cttcgcggga attgtcgtcg gtaaaatgtt tctcgatcgt
      541 ccgcgcgccc agggccacgg cgccgaggac cgtgacgctc ccgggcgtgt ggtcggacaa
      601 ccccggcacg gcgtccggga acagcgcgcg ataaccgttg agcacgttca aatgcacgtg
      661 ccgaaagttt tccaccgacc ccaggtaatt cgtgttgcat tgcatcaacg agaacggcac
      721 ccgggccgcg cgcaacaggc ccacggcccg ctgaacgtcg gcgagggtcg acgcccccgt
      781 ggccaggagg accggtttgc ccttgcccgc cactttcacg atcatttcgg gccaggagat
      841 gtccccggaa ccgattttat agaggtccac gtgggcgtcg agcatgtcca cggcctcgag
      901 gtcgtacggg gccgaaaaat aatcgatctt cgcggcccgg cattccttcg ccagggcctc
      961 ggtccactcc cagggaaggg aggccgcttg atagacttcg gagaccgatt ttttccaacg
     1021 gctctggtgc gatttccccg tccccaattg ccggaagccg tggtcgctga cgatgtggcg
     1081 ggcccggaaa ttttggaatt tcgccgcgtc ggccccgctt tcggccgcca agt
//
LOCUS       DAOQLS010000800         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_117704_length_1132_cov_5.169916, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000800 DAOQLS010000000
VERSION     DAOQLS010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117704_length_1132_cov_5.169916"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PK362_11635"
     CDS             <1..931
                     /locus_tag="PK362_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC75520.1"
                     /translation="LARDGLAVADVAESVLGGVNGLTPTAFRENAKEIPVRLRLREED
                     RKDAAALERLVIPSPFEKGGSHPLNEYGRLSIVPGPSEIRRRDQRRTVLLSVFFSPGG
                     SEKGLAAVRSVLNRFPRKGEVSVALGAEVGEVEASLNSLLIGGVAALGLVYIVLVAQF
                     NVLWLPLLALVSVPLSINGVTPALFVLRNTLNLMSGQGLMVLSGIVVNNSILLLEFIQ
                     QRRAAGMSVEDAVLGASRTRLRPILMTAGTTVIGLLPLALGIGAGAKLQAPMAITVIF
                     GLLVSTGLTLIVLPALYLEARHFFEKGPDVPAN"
     gene            complement(928..>1132)
                     /locus_tag="PK362_11640"
     CDS             complement(928..>1132)
                     /locus_tag="PK362_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75521.1"
                     /translation="LVFYRDADNTSRITEADPLMLLILEHFKSPGARLADLEPLRRRL
                     LPHNAVPLESVLSNLKDRDIVLV"
BASE COUNT          197 a          319 c          379 g          237 t
ORIGIN      
        1 cctggcccgg gacgggttgg ccgtggccga cgtggccgaa tcggttctgg ggggcgtcaa
       61 cgggttgacc cccaccgcct ttcgggagaa cgccaaggaa attcccgtga gacttcgttt
      121 gcgggaggag gaccggaaag acgccgccgc cctggaacgc ctggtgatcc cgagtccctt
      181 tgagaaaggc ggcagtcatc cgctcaacga atacggccgc ttaagcatcg tccccggccc
      241 ctcggaaatc cgccgtcggg atcaacgccg aacggtgttg ctgtcggtgt tcttttcccc
      301 gggcgggagc gaaaagggcc tggccgcggt tcgaagcgtc ctcaaccgtt ttccgcggaa
      361 gggcgaggtg tccgtggccc tgggcgccga ggtgggggaa gtcgaagcgt ccctcaacag
      421 tttgctgatc gggggggtgg cggcgctcgg attggtttac atcgtcttgg tcgcccaatt
      481 caatgtgttg tggctgccgt tgttggccct ggtgtcggtc ccgttgtcga tcaacggcgt
      541 tactccggcc cttttcgtgt tgaggaacac actaaacttg atgtcgggcc agggcctgat
      601 ggtcctttcc gggatcgtcg taaacaactc catattgctg ttggaattca tccagcaacg
      661 gcgggcggcg ggaatgtccg tggaggacgc ggtgttgggg gcgagccgaa cgcgccttcg
      721 gccgatctta atgacggcgg ggaccacggt gatcggcctt ctgcccctgg ccctcggcat
      781 cggcgccggg gcaaagcttc aggcccccat ggccatcacg gtcattttcg gcctcttggt
      841 atcgacgggg ttgacgttga ttgtgttgcc ggcgctctat ctcgaagccc ggcatttttt
      901 cgaaaaaggt cccgacgtcc cggcgaatta aaccagaacg atatcccggt ctttcaggtt
      961 cgacaaaacg gattcaagcg ggacggcgtt gtgcggtagc aaacggcggc gcaacggttc
     1021 caggtcggcc aagcgcgcgc ccggcgattt gaagtgttca agaattaaaa gcatgagcgg
     1081 atcggcttcc gtgatgcggg aggtgttgtc cgcgtcccgg taaaaaacca gg
//
LOCUS       DAOQLS010000801         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_117974_length_1131_cov_2.892193, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000801 DAOQLS010000000
VERSION     DAOQLS010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117974_length_1131_cov_2.892193"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /gene="fliD"
                     /locus_tag="PK362_11645"
     CDS             complement(<1..1122)
                     /gene="fliD"
                     /locus_tag="PK362_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNC75522.1"
                     /translation="MNGPFSASIVNDGTTGTPYRLAIVSKSTGVAGRVDLTTVADPVV
                     NEAVGTGDGSAGAALGDYRLGAPLAAASDLSSLTVGGVAFAARATGANTGVGNEVEVD
                     AATGRLRFFVDGVAANVTGEIRASYTPRALTFTEVQAAQDARIQVGTTSPQTFTSRTN
                     LFTQALPGLTMNLLSANPDKTVEVIVQPNVNAAADAVKKYIDAYNAIVTAVSAQNTYD
                     TEKKQKGGPLFGDALLTTALSQVTQAVTAAVDGLPASINSVFQVGVKAAARGTLSVDG
                     TVLQDALTNRYDDVRNVFLRTTNAARGAAVTASSSAAGYDAGDAVNGEIDSADFGPGA
                     GEVQSRGDARDATSDDRDVGVPGQRGRGGERGGVEQPERG"
BASE COUNT          154 a          393 c          383 g          201 t
ORIGIN      
        1 acctcgctct ggttgctcga cgccgccccg ttctccgccg cgaccccgct gcccgggcac
       61 accgacgtcg cgatcgtcgg aggtggcatc gcgggcatct ccgcggctct gcacctcgcc
      121 cgcgccggga ccgaagtcgg ccgaatcgat ttctccgttc acggcgtctc cggcgtcgta
      181 cccggcggcg gagctcgagg ccgtgaccgc cgcgccccgg gcggcgttcg tcgtgcgcag
      241 gaacacgttc cgcacgtcgt cgtatcggtt cgtgagcgcg tcctggagga ccgtcccgtc
      301 gaccgacaga gtcccccgag cggcggcctt cacccccacc tggaaaacgc tgttgatcga
      361 agcgggcaac ccgtccaccg ccgcggtcac cgcctgggtg acttggctca gcgccgtcgt
      421 cagaagcgcg tccccgaaca ggggcccgcc cttttgtttt ttctccgtgt cgtacgtgtt
      481 ctgggcgctc accgccgtca cgatggcgtt gtaggcatcg atgtattttt tcaccgcgtc
      541 ggccgcggcg ttgacgttgg gctggacgat cacttcgacc gtcttgtccg ggttcgcgga
      601 caacaaattc atcgtgaggc ccgggagggc ctgggtgaac aaattggtgc ggctggtaaa
      661 ggtttggggg gacgtggtgc ccacctgaat ccgcgcgtcc tgggcggcct ggacttccgt
      721 aaacgtcagg gcgcgcggcg tgtaggaggc ccgaatctcc ccggtgacgt tggcggcgac
      781 gccgtccacg aaaaaccgga gccggccggt ggccgcgtcc acttcgactt cgtttccgac
      841 gccggtgttg gcccccgtgg cccgcgcggc gaaggccacg ccccccacgg tgagggaaga
      901 gaggtccgac gcggcggcca ggggcgcccc caaccggtag tcgcccaacg cggcgccggc
      961 ggacccgtcg cccgttccca ccgcttcgtt gaccaccggg tcggcgacgg tggtcagatc
     1021 cacgcgtccc gcgacgccgg tgcttttgct cacgatcgcc aggcggtagg gcgttcccgt
     1081 ggttccatcg ttgacgatgg aggccgaaaa gggaccgttc aaggcgttga t
//
LOCUS       DAOQLS010000802         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_118284_length_1130_cov_1.734884, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000802 DAOQLS010000000
VERSION     DAOQLS010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118284_length_1130_cov_1.734884"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1130
                     /gene="murF"
                     /locus_tag="PK362_11650"
     CDS             23..>1130
                     /gene="murF"
                     /locus_tag="PK362_11650"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNC75523.1"
                     /translation="MIDSRAVQPGDLFVALKGDRRDGHDFLKEVADRGAVGAMVSRSV
                     DGLPSGFGLIRVDDTLQGLQRLGHAHREKMPVRVIGITGSNGKTTTKEMTAHLLQAMG
                     RDVFATRGNLNSQIGLPLMLLELEPHHTHAVLEMGASAKGDIQGLAEMARPQMAVITG
                     IGRAHLETFGSLEGVLAAKWELVESLGKDGIAFLNADDPLLLSRRKSARCSVVTYGLS
                     AGADVRAENVRQDPQTAFDLVVGGARRNVRLPVPGLFNVTNALAAAAVALWERGLLPH
                     VAEGLGRFTPPPQRMQLRRRPDGSMFVIDAYNANPDSMGASLDSFVQAFPHWPKTAVL
                     GSMLELGPASEQEHRQLGRKLAGLPLERIFFVGPE"
BASE COUNT          198 a          318 c          393 g          221 t
ORIGIN      
        1 ccccgcgaat gtcctcaggg tggtgattga ttcccgggcg gtccagccgg gggatctctt
       61 tgtggccctg aaaggcgacc gacgggacgg ccacgacttt ttaaaagagg tggcggatcg
      121 aggggcagtg ggggcgatgg tgagccggag cgtggacggc ctgccttccg ggttcggttt
      181 gatccgtgtg gacgacacgc ttcaaggcct tcaacggctc ggtcacgccc accgggaaaa
      241 aatgccggtt cgggtcatcg gcattacggg atccaacggc aagacgacca caaaagaaat
      301 gacggcgcac cttctgcagg ccatggggcg ggacgtcttt gccacccggg gaaacctgaa
      361 cagccagatc ggtttgccgc tcatgctttt ggaattggaa cctcaccata cgcacgccgt
      421 gctcgagatg ggcgcgtcgg ccaagggcga cattcagggg ctggcggaaa tggcccgccc
      481 ccagatggcg gtgatcacgg gaattgggcg ggcccatctg gagacgtttg ggtcgctgga
      541 aggcgtcctc gccgccaaat gggaattggt ggagtccttg gggaaagacg ggatcgcttt
      601 cttgaacgct gatgacccgt tgctcctgtc tcgtcggaaa tcggcccgct gttcggtggt
      661 cacctatggt ttgtccgccg gggccgacgt tcgggcggaa aacgttcgcc aagatcccca
      721 aacggcgttt gatctggtgg tgggcggcgc tcgccgaaac gtccgcttgc ccgtgccagg
      781 gttgttcaat gtgaccaatg ctctcgcggc ggccgcggtg gccctgtggg aacggggcct
      841 gttgccccac gtggccgaag ggttgggacg gtttaccccg ccgccccaac gcatgcaact
      901 gcgccgtcgg ccggacggtt cgatgtttgt gatcgacgcg tataacgcca accccgattc
      961 catgggcgcc agcctggaca gtttcgtcca ggcctttccc cattggccga agacggcggt
     1021 gttgggaagc atgctggaat tggggccggc gtcggaacag gaacaccggc agttgggccg
     1081 taaactggcg ggactgccgt tggaacggat tttttttgtg gggcccgaag
//
LOCUS       DAOQLS010000803         1129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_118365_length_1129_cov_3.117318, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000803 DAOQLS010000000
VERSION     DAOQLS010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118365_length_1129_cov_3.117318"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          215 a          291 c          389 g          234 t
ORIGIN      
        1 gtcgatgggc cgcaacgtgg cccgttggcg tggccgcttt atatggcaac cttccgccgg
       61 gccggggggc gggccgatga aagagtttag gggcttttgg atttcgggcg cgaaaatccc
      121 ttcggcgttt gaattgcttt tcccctcgcc ccggatgggg agaggccggg agagggatcc
      181 gccggagggt tgtccctttc cccagagggg agagggagga cagggagagg gctccttttg
      241 ggggtggtgt gcgcgcgggg ttgggattga aaaagccctt gcggttcggc gagggctgag
      301 gagcattcac tattcgaaaa agagtatccg cgtggacttc gttttggaga ccccggaatc
      361 ggagagtgac gggggtcgaa cgctcggacg ggacggagac tccgcccgcg ctctcgacgg
      421 gaaaagccag atggaatgga attcttccac cgtcgtcctc ctccggcaga cggaggaaat
      481 tcgaccggtt cgggggttgg atgccggagg aggacgacgt ctcggatggg aacggaagct
      541 ttcaatcggg aaagagaaag gcccaacgac cgtaagccgt tgagcctttt gaccccggtg
      601 gggggtttct gtaatggcgg cgattttaaa aatttgcgaa ctatcgccat cgacttccca
      661 aatgtagcac attcctattg ggataatttc cgcctgactg gggaatatta tatcaaacca
      721 acgcggttga ccctgtagca tggggcttcc gaccgcgcta gcggaagtgc cgcgtgggag
      781 cggccagccc atgtggttct gtggtgcacc ctcatcgttc cgaacggatc gacgaacgtg
      841 ataaagcatt ttccttctcc cgtccgtggg agaaggtgtc ccgaagggac ggatgagggg
      901 cggttttccg ttgaccattc cctcgcccgc tggcgggaga gggccaggga gagggtgggc
      961 gcgtttcacc tcacccccgc cgggggagag gccgggagcg gggggcattt tttttagctc
     1021 caatttgtgc ccgctatacg cgtgccacac cgtgccgctg ttctctccac gtccccgtcc
     1081 aatgaatggg acagtaggca ggacaagacc tggatcttcg gacctagcg
//
LOCUS       DAOQLS010000804         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_118745_length_1127_cov_4.074627, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000804 DAOQLS010000000
VERSION     DAOQLS010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118745_length_1127_cov_4.074627"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..899
                     /gene="cdaA"
                     /locus_tag="PK362_11655"
     CDS             87..899
                     /gene="cdaA"
                     /locus_tag="PK362_11655"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNC75524.1"
                     /translation="MAAFLARLWNGPLIHVVDIGLVALIIYRVLLLIQGTRAVQVLRG
                     LVVLALATLIVENFLHLPTLGWILKTFWLGWAVILAVVFQPELRSFLAQLGSQRLGRV
                     LIPQELRFIDEIIAGLREAAANRVGALIVLEQETGLRNFIATGTPINGEVTSDLLLTI
                     FHPHTPLHDGAAILREDRLVAAGCVLPLSNAPDIARVLGTRHRAALGLTEISDAIVLV
                     VSEETGTVSLAREGRLERDLNVEDLRERLRDLYRSLGERGLLRRRGARGSDV"
     gene            896..1030
                     /locus_tag="PK362_11660"
     CDS             896..1030
                     /locus_tag="PK362_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75525.1"
                     /translation="MSRWGRHWELKIMAVLLATFLWFALRVNAHRPLPTLGPPPTEAR
                     "
BASE COUNT          149 a          382 c          377 g          219 t
ORIGIN      
        1 aaccgcgcgc ccggccgtcg ccgggcccaa tcaaggaggc agtcaccgaa cgcgccggac
       61 gacggcgccg ttccccccat cccgccatgg ccgcatttct cgcccgtctt tggaacggcc
      121 ccctgatcca cgtcgtcgac atcggcctgg tggccctcat catttaccgc gtcctgcttt
      181 tgatccaggg cacccgcgcc gttcaggtgc tgcgcggtct cgtggttctc gccctggcga
      241 ccctcatcgt cgaaaatttc ctccatttgc cgaccctggg atggattctt aaaacctttt
      301 ggctcggctg ggccgtcatc ctggccgtcg tgttccaacc ggagcttcgg tcgtttctcg
      361 cccagttggg cagtcagcga ttgggccggg tgttgatccc ccaagagctt cggttcatcg
      421 atgagatcat cgccggcctt cgggaggccg cggccaaccg cgtgggcgcg ctgatcgtcc
      481 tggagcagga gacggggctt cggaacttca tcgccacggg cactcccatc aacggggaag
      541 tgaccagcga tttgttgctc accatttttc acccgcacac ccctctgcac gacggggcgg
      601 cgatccttcg ggaggaccgt ctggtcgcgg ccgggtgcgt gctgcccctg tccaacgcgc
      661 cggacatcgc ccgggtcctg ggcacccgcc accgggcggc cctgggcctc acggaaattt
      721 cggacgcgat cgtgctcgtg gttagcgagg aaaccggcac ggtttccctg gcccgggaag
      781 gccgcttgga acgggatttg aacgtggagg atttgcggga gcgcctgcgg gatctttacc
      841 ggtccctggg cgagcggggc ctccttcgcc ggcgcggcgc ccgggggagc gacgtatgag
      901 ccggtggggg cgtcactggg agttgaaaat catggccgtt ctgctggcga cctttctttg
      961 gttcgccctt cgcgtcaacg cccaccggcc gttgccgacg ttggggccgc cgccgacgga
     1021 ggcccgctga tggcgaaact tttcggaacc gacggcgtgc ggggcgtggc cggccgctgg
     1081 ccgctcaccc ccgatttcgt gcggcggctc ggttgggccg cggggcg
//
LOCUS       DAOQLS010000805         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_119469_length_1124_cov_3.045837, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000805 DAOQLS010000000
VERSION     DAOQLS010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119469_length_1124_cov_3.045837"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK362_11665"
     CDS             <1..402
                     /locus_tag="PK362_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNC75526.1"
                     /translation="VLTAARQAGVFDRPAADAVARAAQRPLVNLCGETPVPLAAALLR
                     RAAALVTNESGLMHLGWAVGTPTVIVAGPSDPQATSPFGPRVRVIQRREIPCVPCVRN
                     ECRRRGEETNLCLKAVTPEEVLEALAEITTR"
     gene            complement(520..>1124)
                     /locus_tag="PK362_11670"
     CDS             complement(520..>1124)
                     /locus_tag="PK362_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNC75527.1"
                     /translation="MLHATRPSFGGNLMATILCEKHRPEMATVRPMAFPRAPKQAGRQ
                     GKVINVAVDLAKIKQRTRFVRYEAEKGEAQDITSAEVIVAGGRGVGGAEGFKSIEALA
                     NLLKGAVGASRAAVDAGWIAYRHQVGLTGRTVRPKLYIAAGISGQIQHLAGMSSSEVI
                     VSINKDAEAPLNKMATFSIDGDLFEILPALVKEIEKRRGH"
BASE COUNT          181 a          381 c          366 g          196 t
ORIGIN      
        1 gtcctcaccg ccgcccgaca ggctggcgtg ttcgaccgcc cggcggccga cgcggtggcc
       61 cgggccgccc agcgccccct ggtcaatctc tgcggggaaa cccccgtccc cctggcggcg
      121 gcgctccttc ggcgcgcggc cgccttggtc acgaacgaat cgggattgat gcacctgggg
      181 tgggcggtcg gaacgcccac cgtcatcgtt gccggcccct cggaccccca ggcgacatcg
      241 cccttcggcc cccgggtccg ggtgatccag cgccgggaaa tcccctgcgt gccctgcgtg
      301 cgcaacgaat gccggcggcg cggcgaggaa accaatcttt gcctgaaggc cgtgactccc
      361 gaggaagttc tcgaggccct ggccgaaatc accacgcgtt agccgctgcc cgcgggcaag
      421 ggcccttcgg gcgtgattcg atcctggaca ggaagggaag gacgcggggg ggaaacggaa
      481 ggccggcgcc gcgggagcgg tccccggcca aggcgggggt cagtgacccc ggcgtttttc
      541 gatttctttg acgagggcgg gaagaatttc gaacaggtcg ccgtcaatgg agaaggtcgc
      601 cattttgttc aacggcgcct cggcgtcctt gttgatggag acgatcactt cgctggagct
      661 cattcccgcc aaatgctgga tttggccgga aattcccgcg gcgatgtaaa gcttgggacg
      721 cacggtgcgt ccggttaaac cgacttgatg gcggtacgcg atccaacccg cgtccacggc
      781 cgcccggctg gccccgaccg cgcccttcaa caggttggcc aaggcctcga tcgacttaaa
      841 tccctccgcc ccgccgacgc cgcgcccccc cgccacgatc acttcggcgg aagtgatgtc
      901 ctgggcttcg cctttttcgg cttcgtaacg gacaaaacgg gtgcgctgtt tgattttcgc
      961 cagatccacg gccacgttga tcactttccc ctggcgaccg gcttgcttcg gcgcccgggg
     1021 aaaggccatg ggacgaacgg tggccatttc cgggcggtgt ttttcacaca aaatcgtcgc
     1081 catcaaattg ccgccgaacg acggccgggt ggcgtgaagc atcc
//
LOCUS       DAOQLS010000806         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_119641_length_1123_cov_4.916667, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000806 DAOQLS010000000
VERSION     DAOQLS010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119641_length_1123_cov_4.916667"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..>1123
                     /locus_tag="PK362_11675"
     CDS             167..>1123
                     /locus_tag="PK362_11675"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNC75528.1"
                     /translation="MAIPRSFTPTMDNEAFRKESAPSVTKLTAYASPTTGASLFQLFN
                     TAIPFGIMWYLMLKSLSVSYALTLLLAVPTALFLVRLFIFQHDAGHGSFFQSKKANDW
                     VGTLIGILTLTPYSYWQRTHAIHHATSGNLDKRGFGDIDTLTVAEYRSLSPSLKLRYR
                     LYRHPLTMFIVGPFIQFVFKHRFPYNIPFSWKVEWRSVIYNNLGLLAIGILAANTIGL
                     KAFLLVQVPISYLACSLGAWLFYIQHQYENTYWERNDKWDYFDASVRGSSYYVLPKIL
                     QWFTGSIGLHHIHHYNSRIPNYRLQKCYNENPEFQAVTRLSLW"
BASE COUNT          237 a          351 c          260 g          275 t
ORIGIN      
        1 aaagcgtcca ggacatcggg gggaaggcct tcggtgggtt tcatggttct cccgggcgat
       61 cgtcgccgtt ttccccggtg gttcgaggaa tccctggcgt cgtcgtcgaa ttagatataa
      121 aatggtgctt caatccacaa attttaaaac attaagaggt ccgccgatgg ccattccccg
      181 ctcctttacc cccaccatgg acaacgaagc gtttcgaaaa gagagcgctc ccagcgtcac
      241 gaaactgacc gcttacgcga gcccgaccac cggcgccagc ctattccaac ttttcaacac
      301 ggccatccct ttcggaatca tgtggtatct catgctgaag agtctttccg tttcctacgc
      361 cttgacccta ttgctcgccg ttcccacggc cttgttcctg gtgcgtcttt ttattttcca
      421 gcacgacgcc ggccacggat ctttttttca atcgaaaaaa gccaacgatt gggtggggac
      481 cctcatcggg attctgacgc tgacgcctta cagttattgg cagcgcaccc acgccatcca
      541 ccacgcgact tccggcaact tggacaaacg cgggttcggg gacatcgaca ccttgaccgt
      601 ggccgaatac cgcagcctct cgccgtcgct caagttgcgg tatcgcctct accgccaccc
      661 gctgacgatg tttatcgtcg gcccctttat tcaattcgtt tttaaacacc ggtttcccta
      721 caacattccc ttttcgtgga aggtcgaatg gcggagcgtg atttacaaca acctgggtct
      781 cctggccatc gggatcctcg cggcgaacac catcggatta aaggccttcc tcctcgtgca
      841 ggttcccatt tcctacctgg cctgttccct cggggcctgg cttttttaca ttcagcatca
      901 atacgaaaac acctattggg aacggaacga caaatgggat tatttcgacg cctcggtgcg
      961 gggaagttcc tattacgtcc ttcccaaaat cctccaatgg ttcactggca gcatcgggct
     1021 acaccacatt caccactaca acagccggat tcccaattat cggttgcaga aatgctacaa
     1081 cgagaacccc gaattccagg cggtcacccg cttgtccctc tgg
//
LOCUS       DAOQLS010000807         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_119827_length_1122_cov_4.966261, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000807 DAOQLS010000000
VERSION     DAOQLS010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119827_length_1122_cov_4.966261"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..>1122
                     /locus_tag="PK362_11680"
     CDS             102..>1122
                     /locus_tag="PK362_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNC75529.1"
                     /translation="MRFLRLGLVLALLPLAPLRADRRPPYVPPLLRIGLLKQTTAVDL
                     GPGDYLLEGPVPKKRLTLKKPARLQIAGAGFLLNKARWGASVTIAPTTPADEVWVNGR
                     PYRGRLEVTRTGPAFRVVNLLSVEDYLRGVLQMETSDTWPEQALKAQAVISRTYALRN
                     RGRHGRQGYDFCREPHCQTYGGAQAERRLTDAAVDATRGEVLVDRKHRLVGAVYHSTC
                     GGSTESAENVWERGGQSYLVPVSCKWCRGSPHYTWMAKVSNELVTRSLEAGGVHVGTV
                     RALGILSHTPSGRVYRVRVYGDRATVDLKANAFRNLVDPRLIRSTFWSGLSKRGNVWQ
                     IHGRGW"
BASE COUNT          172 a          393 c          359 g          198 t
ORIGIN      
        1 gggccttccc gcgccgaagg accccgcggt ttccccgacc ctcttcgccc cgtgaggccg
       61 ccgttgccgg ccccattcga ccgtcgaccg taaatctccc cgtgcgcttc cttcggttgg
      121 gtttggtgtt ggcgttgttg cccctggcgc cccttcgggc ggaccgccgc cccccctacg
      181 ttcccccgct cctccgcatc ggccttttga aacaaacgac ggcggtcgat ttgggccccg
      241 gcgattatct gttggaaggg cccgtcccca aaaaacgctt gacgctgaaa aaacccgccc
      301 gccttcaaat cgccggggcc gggttccttt tgaacaaagc ccggtggggg gcctcggtga
      361 ccatcgcccc gacgactccc gccgacgagg tttgggtcaa cggacggccc taccggggcc
      421 gcctggaggt aacgcgaacg ggcccggcct tccgcgtcgt gaaccttctt tccgtggagg
      481 attatctccg gggggttctc caaatggaaa cctccgacac ctggcccgaa caggcgctca
      541 aggcccaggc ggtgatcagc cgcacctacg ccctgcgcaa ccggggtcgc cacggccgcc
      601 aggggtatga tttttgccgg gagccccact gccaaaccta cggcggcgcc caggccgaac
      661 gccgcctcac cgacgccgcc gtggacgcca cccggggcga agtgctcgtg gaccggaaac
      721 accggctggt gggcgcggtg tatcattcga cttgcggcgg atccacggaa tccgccgaaa
      781 acgtgtggga gcggggcggg caatcctacc tcgtcccggt ctcctgcaag tggtgccggg
      841 ggtcccccca ttacacctgg atggcgaaag tctccaacga gctggtcacc cgatccctgg
      901 aggccggcgg cgttcacgtg gggacggtgc gggccctggg cattttgtcc cacaccccct
      961 ccgggcgtgt ttaccgcgtc cgggtctacg gcgaccgcgc cacggtggat ctgaaggcca
     1021 acgcctttcg aaacctggtg gacccgcgcc tcattcgaag cacgttttgg tcgggtcttt
     1081 ccaaacgggg aaacgtgtgg cagattcacg ggcgggggtg gg
//
LOCUS       DAOQLS010000808         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_120158_length_1121_cov_2.545966, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000808 DAOQLS010000000
VERSION     DAOQLS010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120158_length_1121_cov_2.545966"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1121
                     /locus_tag="PK362_11685"
     CDS             <1..>1121
                     /locus_tag="PK362_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNC75530.1"
                     /translation="KDHPLRGSEKDRCKNFFALGLMFWLYDRKLDHTIDWISQKFAKK
                     PEFVNANIAALKAGYNHADITELMPTRYEIRQSKLAPGTYRKITGNEATALGLIAAAE
                     LSGKTLFYGSYPITPASGILEGLSKHKEFGVKTFQAEDEIAAIGSAIGASFGGALGVT
                     GTSGPGLCLKSEALGLAVITELPLVVVDVQRTGPSTGMPTKTEQTDLLQALFGRNGES
                     PVCVIAPRSPADSFDMAVEACRLAMTHMTPVVLLTDGYLANSAEPWKLPEIKDLPKII
                     VNHPTQKGAFAPYARDPQTLARPWAIPGTPGLEHRIGGLEKQNITGSVSYDPANHEKM
                     VQLRAEKVARIADDIPPLDVRGPAKGKVLVLGWGSTYGT"
BASE COUNT          252 a          361 c          327 g          181 t
ORIGIN      
        1 aaagatcacc cgctccgggg gtcggagaaa gaccggtgca aaaacttttt cgccctgggg
       61 ctcatgttct ggctctacga ccggaaactc gaccacacca tcgactggat ttcgcaaaag
      121 ttcgccaaga aaccggaatt cgtcaacgcc aacatcgcgg cgctcaaagc cggatacaac
      181 cacgcggaca tcaccgagct gatgcccacc cgctacgaaa tccgccaatc caaactggcc
      241 cccggcacct accgcaaaat cacggggaac gaagccaccg ccctgggcct gatcgcggcg
      301 gcggaactgt ccggcaaaac gcttttctac ggatcgtatc ccatcacccc ggcctccggc
      361 atactcgagg ggctctcgaa acacaaagag ttcggcgtca aaacgttcca ggcggaagac
      421 gagatcgcgg ccatcgggtc cgccatcggc gccagtttcg gcggcgcctt gggcgtgacc
      481 ggaacctcgg gccccggctt gtgtttgaaa tccgaagcgc tgggcttggc ggtcatcacg
      541 gaactgccgc tggtggtggt ggatgtccaa cgcaccggtc cttcgacggg gatgcccacc
      601 aagacggaac agaccgacct tcttcaagcg ctgttcgggc gcaacgggga atcgccggtc
      661 tgcgtgatcg cgccgcggag cccggcggat tcgttcgaca tggccgtgga agcctgccga
      721 ttggccatga cccacatgac cccggtggtc ttattaaccg acggctattt ggccaacagc
      781 gcggaaccct ggaaacttcc ggagatcaaa gacctcccca aaataatcgt gaatcacccc
      841 acccagaagg gggccttcgc cccctatgca cgagaccctc aaacgctggc gcggccttgg
      901 gcgattcccg ggacgcctgg attagagcac aggatcgggg ggttggaaaa gcaaaacatc
      961 acgggatcgg tcagctacga tcccgccaac cacgaaaaaa tggtgcaact gcgggcggaa
     1021 aaagtggccc gaatcgccga cgacattcct ccgctggacg tccgcggccc ggccaagggg
     1081 aaggtcctcg tgctcggttg gggatccact tacggcacga t
//
LOCUS       DAOQLS010000809         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_121234_length_1116_cov_3.975495, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000809 DAOQLS010000000
VERSION     DAOQLS010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121234_length_1116_cov_3.975495"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..675
                     /locus_tag="PK362_11690"
     CDS             67..675
                     /locus_tag="PK362_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75531.1"
                     /translation="MTSSRKDIDLRKHREPDLVAALFHPTEDLRQAAFREICARGEKR
                     GWEAFSFLTQDPELAGRFASIKDPFDAVGIAVAPETYDRIHAVWPKGVEHLGEGGRRE
                     LTIDFRNQRLDVMTIAAGAWPPGEKYPAGQEGFCEVEFRVRDVAAFARRVEELPPELK
                     SRLVLLPADPVTAQSPGAGRWLLVHARAARGARTVLVQLVPA"
     gene            complement(677..>1116)
                     /locus_tag="PK362_11695"
     CDS             complement(677..>1116)
                     /locus_tag="PK362_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75532.1"
                     /translation="GGRAAGLFARAVARATDDRTAAALEGERAEAEFADGRTAQGLDA
                     LARAVARDGDNEGLRTRLAVEWEKNARPLEAARQWTFLRDRHPTEKTYHWRLTQAWMM
                     AREPVKAFAAVERYLALPLEADERRAAEEFRKRLAESAAQAAR"
BASE COUNT          156 a          403 c          369 g          188 t
ORIGIN      
        1 tggttcgcgg ggcgctcgaa tgacgtttcg ccgattcgtt ccccgcgagg aacgtgcgag
       61 gccccaatga cctcttcccg caaagacatc gaccttcgta aacaccggga gccggacctt
      121 gtggcggccc tgttccaccc caccgaagac ctccgccagg ccgccttccg ggaaatttgc
      181 gcccgggggg agaaacgcgg ctgggaggcc ttctccttcc tcacccagga ccccgaactg
      241 gccggccgct tcgcgtcgat caaagatcct ttcgacgcgg tgggcatcgc cgtggcgccc
      301 gagacctacg atcggattca cgcggtgtgg cccaaggggg tggagcattt gggggagggg
      361 gggcgacggg aattgaccat cgatttccga aaccagcggt tggacgtgat gaccattgcc
      421 gccggggcgt ggccgccggg ggaaaaatac cccgccggcc aggaagggtt ttgcgaagtg
      481 gagtttcggg ttcgggacgt ggcggcgttc gcccggcggg tggaggaatt gcccccggaa
      541 ttaaaaagcc ggttggttct ccttcccgcc gacccggtga cggcccaaag cccgggcgcc
      601 ggccgatggc tcctggtaca cgcccgggcg gcccggggcg cccgcacggt gctcgtccag
      661 ctggtccccg cctagattac cgcgcggcct gcgccgcgct ttccgccagc cgttttcgaa
      721 attcctcggc cgcccgccgt tcgtccgctt ccaggggcag ggccaagtac cgttccaccg
      781 ccgcgaaggc tttgaccggt tcccgggcca tcatccaggc ctgggtcaag cgccaatggt
      841 aggttttctc cgtcgggtgg cgatccctca aaaaagtcca ttgccgggcc gcttccagcg
      901 gccgcgcgtt cttctcccac tccaccgcca gccgcgttcg cagcccctcg ttgtccccgt
      961 cgcgcgccac cgcgcgcgcg agcgcgtcca acccctgggc cgttcggccg tcggcgaatt
     1021 cggcctcggc ccgttcccct tccaacgccg cggccgtccg gtcgtcggtc gcgcgggcca
     1081 cggcccgggc gaacagcccg gccgcgcggc cgccgt
//
LOCUS       DAOQLS010000810         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_121474_length_1115_cov_3.225472, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000810 DAOQLS010000000
VERSION     DAOQLS010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121474_length_1115_cov_3.225472"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..>1115)
                     /locus_tag="PK362_11700"
     CDS             complement(113..>1115)
                     /locus_tag="PK362_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNC75533.1"
                     /translation="GTELRGPFTKFGPRDTRRWFESRGVALKTEEDGRVFPVTDRSQT
                     IVDALTAAAQGANVEVRTGAAVRAIRPDGTGFQLDLNSGESLSAARVLLATGSNPQGH
                     AWARALGHTIVPPVPSLFTFTIKDPRLKGLAGVASPRARVKLEGTSLEQAGPVLITHW
                     GLSGPAVLRLSAWGARVMHEGGYRGTLRVNWLDQSPEVLQKTFDQKRKTDPRKSLSAS
                     PFDIPQRLWDSLRTAAGLPAERRWADLSKPEMARLGAELGDGAYAVTGKSAFKDEFVT
                     SGGVALEEVDFRTMESRRRPGLYFAGEILDIDGVTGGFNFQNAWTTGWLAGRALARR"
BASE COUNT          187 a          357 c          372 g          199 t
ORIGIN      
        1 cctcaagggg ggaaggagac ccccggtgga ttttgccggg cacctaaggg cggtttgatt
       61 taaataaaaa aaagtttggg cctcgcggcc cgggcttttt ttttagcccg acttaccgcc
      121 gggccagggc tcggcccgcc agccagcccg tggtccaggc gttttgaaag ttgaacccac
      181 ccgtcacgcc gtcgatgtcc agaatttcgc ccgcgaagta aagaccgggc cgccggcggc
      241 tctccatggt tcgaaaatcc acttcctcca gggccacccc gcccgaggtc acgaattcgt
      301 ctttaaaggc gcttttgccg gtgacggcgt aggccccgtc cccgagctcc gcccccaggc
      361 gggccatttc cggttttgag aggtccgccc aacggcgttc ggcgggaagg cccgcggcgg
      421 ttcgcaggga atcccagaga cgttggggaa tatcaaaggg ggaggcggaa aggctttttc
      481 gcgggtcggt ttttcttttt tggtcgaagg ttttttgaag gacctccggg gattggtcca
      541 accaattcac ccgaagggtc ccgcgatagc cgccctcgtg catgacccgg gcgccccagg
      601 ccgacaggcg caggaccgcc gggccgctca ggccccagtg ggtgatcaac accgggcccg
      661 cttgctccag ggacgtcccc tccaatttca cccgggcccg gggcgaggcc acgcccgcca
      721 atcctttaag acgcgggtct ttgatggtga aggtgaacag ggagggaacg ggggggacga
      781 tggtgtgccc cagggcccgg gcccaggcgt gtccctgggg attgctgccc gtggcgagaa
      841 gaacccgggc ggccgagagg gattccccgg aattcaaatc cagttgaaag cccgtcccgt
      901 cgggtcgtat cgcgcgcacc gccgcgccgg tccgcacctc gacgttcgcc ccttgggccg
      961 cggcggtcaa ggcgtcgacg atggtttggg agcggtcggt gacgggaaat acccggccgt
     1021 cctcttcggt tttcaaagcc acgccccggg actcgaacca ccgccgggtg tcccgggggc
     1081 cgaacttggt gaagggtccc cgaagctccg tcccg
//
LOCUS       DAOQLS010000811         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_121710_length_1114_cov_2.913126, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000811 DAOQLS010000000
VERSION     DAOQLS010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121710_length_1114_cov_2.913126"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK362_11705"
     CDS             complement(<1..193)
                     /locus_tag="PK362_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75534.1"
                     /translation="MDLLERRKVLELLLWLTDKPLKTTELTAVLGADAPTELELREEL
                     NGLARELDERGAPYHLMEIA"
     gene            complement(194..976)
                     /locus_tag="PK362_11710"
     CDS             complement(194..976)
                     /locus_tag="PK362_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNC75535.1"
                     /translation="MTTAGNNVKLDVFEGPLDLLLYLIKKNDLNIHDIPLSQITNDFL
                     QYLGLLKDLNLNIAGEFLVMAATLMQIKARTLLPAPSREDEETVDPRADLVHRLLEYQ
                     RFKEASRHLESRLHATKDIHFRGSPTFGETDYTMDASLFDLLDAFRDVLKNLRPRVRE
                     IESEDVPIEVKIKDVLAFLSGRPYATFREILERETTRRGLIITFLAILELIRLKEIAA
                     RQSEAFAEIRVYRRDALPENLETDGAAPEPEPAPAAPLPEGE"
BASE COUNT          186 a          331 c          368 g          229 t
ORIGIN      
        1 gggcgatttc catcaaatgg tacggggccc cccgctcgtc caactcccgg gcgaggccgt
       61 tcaattcctc ccgcaattcc aattccgtgg gcgcgtcggc gcccaacacg gcggtcaatt
      121 ccgtcgtttt gagcggcttg tcggtgagcc acagcagcag ttcgaggact tttcgtcgtt
      181 ccagcaaatc catctattcc ccctcgggaa ggggcgccgc gggcgcgggt tcgggctccg
      241 gcgcggcgcc gtcggtttcc aggttttccg gcagggcgtc ccgccggtac acgcgaattt
      301 cggcgaaggc ctcgctttgt cgggcggcga tctccttcaa ccggatcaat tccaaaatgg
      361 ccagaaaagt gatgatgagc ccccggcgcg tcgtttcccg ctccaagatt tcgcggaagg
      421 tggcgtaggg gcggccggac aggaaagcga ggacgtcctt gatcttgact tcgatcggca
      481 cgtcctcgga ttcgatctcc cgcacccggg gccgcaggtt tttgagcacg tcccggaagg
      541 cgtccaacaa atcgaagagc gaggcgtcca tggtgtagtc cgtctcgccg aaggtgggcg
      601 acccccggaa atggatgtcc ttggtggcgt gcaggcggct ttcgaggtgg cgggacgcct
      661 ccttgaaccg ctggtactcc agaaggcggt ggaccaagtc ggcccggggg tcgacggttt
      721 cctcgtcctc ccgggacggg gcgggcagca gggtccgggc cttgatctgc atgagcgtcg
      781 cggccatcac cagaaattcg ccggcgatgt tcaaattcaa atctttcaac agccccaggt
      841 attgaaggaa atcgttggtg atctgggaca ggggaatgtc gtggatgttg agatcgtttt
      901 tcttgatgag gtagagcaga agatcgaggg gcccctcgaa cacgtccagc ttgacgttgt
      961 tgccggcggt ggtcatggaa gtcccatggc ggctttggcc tcgtccaagg tggcccgagc
     1021 gacgcggcgg gcggcccggg tgccttcttc caggatctcg cggacccaac ccggcttttg
     1081 cgccaggacg gcgcggcgct cctgaatttc tttc
//
LOCUS       DAOQLS010000812         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_122121_length_1112_cov_3.109745, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000812 DAOQLS010000000
VERSION     DAOQLS010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122121_length_1112_cov_3.109745"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1112
                     /locus_tag="PK362_11715"
     CDS             60..>1112
                     /locus_tag="PK362_11715"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004396 - hexokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexokinase family protein"
                     /protein_id="HNC75536.1"
                     /translation="MVEALKTLRSAAYYFGRRVNVLVTALGPVSVGRPQSAWRQISGK
                     YFNAALFKLTHLVQGLERVEREAGKAEFERRFATAAAGAFGPAWPLDRDDVLIRLAAR
                     VRSDIDAARRGQPSSASRWPANLPVLSGNERGRVLVIDWGGTRVKFRVVDLEGAGRYR
                     IGEASEFSFTAAEKRGEGERLFDRVAEAAQKIAAETPGLERAAFVFSFPVKPTALDAG
                     QLTRWTKEITATGVEGRDVVALLREALARRGASLKVKALVNDAVGALAGISYADAADG
                     RPFETADAGLILGTGVNAAVRWDGDAHNLELGQWNLGAALRAADPAGAEVWDRFLSIS
                     PTLERETASLYLGETLR"
BASE COUNT          171 a          359 c          397 g          185 t
ORIGIN      
        1 cccgccggtc ggtcctggcc gcagcgggga acggcgggtt ggggtcgaag tatccggcga
       61 tggtcgaggc cctgaaaacc ctccgctccg cggcctatta tttcgggcgg cgggtcaacg
      121 tgttggtgac ggccctgggc cccgtgtccg tgggccgccc ccaatccgcc tggcggcaaa
      181 tttcaggaaa atatttcaac gcggcgctgt tcaagttgac gcatttggtg caagggttgg
      241 agcgcgtcga acgggaagcc ggcaaggccg aatttgaacg ccgtttcgcc acggccgccg
      301 ccggggcctt cggcccggcc tggcccctgg atcgggacga cgttttgatc cggttggccg
      361 cccgggtccg ttccgacatc gacgccgcgc gacggggcca accgtcgtcg gcctcccgtt
      421 ggccggcgaa cctccccgtc ctttccggca acgaaagggg acgggtgttg gtgatcgatt
      481 ggggcggaac ccgcgttaaa ttccgcgtcg tggacttgga gggcgccggc cgctaccgca
      541 tcggggaggc gtcggaattt tctttcaccg ccgccgaaaa acggggcgag ggggagcggc
      601 ttttcgaccg ggtggccgaa gcggcccaaa aaatcgcggc cgaaacgccg ggcctcgaac
      661 gagccgcctt tgtcttctct ttcccggtga aacccaccgc cctggacgcc ggacaattga
      721 cccggtggac gaaggaaatc acggccacgg gcgtggaagg ccgggacgtg gtcgcgcttc
      781 tgcgggaggc tctggcccgg cggggagcct ctttaaaggt gaaggctttg gtcaacgacg
      841 ccgtgggcgc cctggcgggg atctcctacg ccgacgcggc tgacggacgc ccgtttgaaa
      901 cggccgacgc ggggctcatc ctcggcaccg gcgtcaacgc cgccgtccga tgggacggcg
      961 acgcgcacaa tttggagctg ggtcagtgga atctgggggc cgccctccgg gcggccgacc
     1021 ccgcgggggc cgaggtgtgg gaccgcttcc tttccatctc gccgacgctc gagcgggaaa
     1081 ccgcctccct gtacctggga gaaacactgc gc
//
LOCUS       DAOQLS010000813         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_122172_length_1112_cov_2.489120, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000813 DAOQLS010000000
VERSION     DAOQLS010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122172_length_1112_cov_2.489120"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /gene="trpD"
                     /locus_tag="PK362_11720"
     CDS             complement(<1..544)
                     /gene="trpD"
                     /locus_tag="PK362_11720"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNC75537.1"
                     /translation="MIHSAVGKLVEKIDLTFDEARESVADIFSGTVPASQMAGFLIAL
                     RMKGETVDEIAGAADAMRGAATRIHLPNTPVIADTCGTGGDKQGSFNISTAAAFAVAG
                     AGFTVAKHGNRSISSQCGSADVLEALGVKLDPPAAVVERCLKEIGIGFLFAPAFHPAM
                     KHALPVRRELGVRTVFNMLGP"
     gene            complement(541..930)
                     /locus_tag="PK362_11725"
     CDS             complement(541..930)
                     /locus_tag="PK362_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNC75538.1"
                     /translation="MGTETARKLRNNPTKTERILWKQLRQRGVKGHRFRRQHPMGPYV
                     LDFVCLEQKLVIEVDGGQHAKDVRSDAQRTFWLNKNGFKVLRFWNHEVLQNLEAVISV
                     IRSSLIDTDASPHLSPPPQGGRTEASL"
     gene            complement(977..>1112)
                     /locus_tag="PK362_11730"
     CDS             complement(977..>1112)
                     /locus_tag="PK362_11730"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514249.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HNC75539.1"
                     /translation="EDGEIMGVRHRTVPALEGVQFHPESILTEGGKDLLKNFLGFKVN
                     "
BASE COUNT          247 a          339 c          281 g          245 t
ORIGIN      
        1 aagggcccaa catattaaat acggtccgaa ccccgagttc ccgacggacc ggcagggcgt
       61 gtttcatggc cggatgaaac gccggggcga acaaaaaacc gatgccgatt tctttcaggc
      121 accgttccac cacggcggcc ggcggatcca gtttgacgcc caaggcttcc aacacatcgg
      181 cactcccgca ttgggacgag atggaccgat tgccgtgttt ggccacggtg aatcccgccc
      241 ccgccaccgc gaaggccgcc gccgtggaaa tattaaagct tccttgttta tctccccctg
      301 tgccacaagt atccgcgatc acaggcgtgt tcggtaaatg aatccgcgtc gcggcgcccc
      361 gcatggcgtc cgcggccccg gcgatctcgt cgactgtttc gcctttcatg cgaagagcga
      421 tcaaaaaccc cgccatctga ctggccggca cggtccccga aaaaatatcg gccaccgact
      481 cccgcgcctc gtcaaaggtc aggtcgattt tctccaccaa cttccccaca gcggagtgga
      541 tcataaagag gcctctgtcc tccccccttg agggggagga gaaaggtggg gggaagcatc
      601 agtgtctatc aaagacgaac gtatcacaga aatcaccgct tcaaggtttt gtaaaacttc
      661 gtggttccaa aatcgcaaca ctttaaaccc atttttattt agccagaaag ttctctgcgc
      721 atcggacctt acgtccttag catgttgccc gccatccact tcaatcacca acttctgttc
      781 caagcataca aagtcaagaa cgtagggccc catggggtgt tgacggcgaa aacgatggcc
      841 tttaacacct ctctgtcgca attgcttcca aagaattcgt tctgttttgg tcgggttatt
      901 tctaagcttt cgtgcggtct ccgttcccaa agcccccccc catcccgacc ttccccctcg
      961 aggggggaag gagaaatcaa ttgaccttga aacccaagaa attttttaaa aggtcttttc
     1021 ccccctcggt gaggatgctt tcggggtgga attggacgcc ttccagggcg gggacggtgc
     1081 ggtggcggac gcccatgatt tcgccgtctt cg
//
LOCUS       DAOQLS010000814         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_122971_length_1109_cov_2.077799, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000814 DAOQLS010000000
VERSION     DAOQLS010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122971_length_1109_cov_2.077799"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /gene="murA"
                     /locus_tag="PK362_11735"
     CDS             <1..1001
                     /gene="murA"
                     /locus_tag="PK362_11735"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNC75540.1"
                     /translation="YDLVRRMRASAVVMGPLLARLGQADVSLPGGCAIGARPINFHLK
                     AFETMGARITVHEGYISAVGRLKGSRVKLPFASVGATENVLMAASLAQGRTVIENAAR
                     EPEITDLADLLAAMGAVINGAGTDRITVDGVPALHGARHRVIPDRIEAGTFLVAAAMT
                     RGRVRLTDADPGHLTAVLTALRKAGLRVTVGPSTVEAAWVRPLKPLSIKTKVYPGFPT
                     DMQAQWMALMAVTPGRSVIEEDIFENRFLHAQELLRMSARIETRGHRAVVEGGARLSG
                     CPLMVSDLRAGAALVLAGLAARGTTKVLRVYHLDRGYEKLEKKLRALGADIRRVPQ"
BASE COUNT          182 a          326 c          387 g          214 t
ORIGIN      
        1 cctacgattt ggttcggcgc atgcgggcca gcgcggtggt catgggcccg ctcttggcgc
       61 ggctggggca ggccgacgtg tccttgcccg gcggatgcgc catcggcgcg cggcccatca
      121 actttcatct caaggccttt gaaaccatgg gcgctcgcat cacggttcat gaaggctaca
      181 tttccgccgt gggccgactg aaaggatccc gggtaaaact gcccttcgcc agcgtgggcg
      241 ccacggagaa cgtgctgatg gcggcttctc tcgcccaggg gcggacggtg atcgagaacg
      301 ccgcgcggga accggagatc acggatctgg ccgatctctt ggccgccatg ggcgccgtga
      361 tcaacggcgc ggggactgat cgcatcaccg tcgatggagt gcccgctttg cacggggcgc
      421 ggcatcgggt gatcccggac cggatcgagg cgggcacgtt tctggtggcc gcggccatga
      481 cgcgcgggcg ggttcgactg accgacgcgg accccggcca tttgacggcg gttttgacgg
      541 cccttcgaaa ggccggcttg cgcgtgacgg tgggcccaag cacggtggag gccgcctggg
      601 tgcgcccctt gaaacccctg tcgatcaaaa ccaaggttta tccggggttt ccgacggaca
      661 tgcaggctca atggatggcg ctcatggccg tgacgcccgg ccggagcgtg atcgaggaag
      721 atatttttga aaaccggttt ctccacgccc aagagcttct gcgcatgagc gcccggatcg
      781 agacccgggg ccatcgggcg gtggtggaag gcggggcgcg cctgtcgggc tgtccgctca
      841 tggtgtcgga tttgcgggcc ggggcggccc tggttttggc ggggctcgcc gcccggggaa
      901 cgaccaaagt tctccgcgtt taccatttgg atcgggggta tgaaaaactc gagaaaaaat
      961 tgcgcgccct cggcgcggat attcggcggg ttccccaatg aaatcacaga agggcttcca
     1021 ctgtttttcc cgtcatcccg gcgaaagccg ggatccagag ggttttgttt tttttgaaag
     1081 aagtttcagg gactggatcc cggctttcg
//
LOCUS       DAOQLS010000815         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_123067_length_1108_cov_3.895537, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000815 DAOQLS010000000
VERSION     DAOQLS010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123067_length_1108_cov_3.895537"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1108
                     /locus_tag="PK362_11740"
     CDS             <1..>1108
                     /locus_tag="PK362_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNC75541.1"
                     /translation="SAAAALLERHKDLPASGHAEETVAVSGRVVTCRDMGKTIFAHLQ
                     DLSGKIQIYFKKDELPEAFLAFQKTVDSGDIVGVEGSPFRTRTGEITVHVKSWRLLAK
                     ALRPPPEKFHGMTDVEIRYRRREVDLFSNPEARERFLDRQRIVSALREVLLQKNFIEV
                     ETPILQAVAGGAAAKPFVTQHHALGMELFMRIAPELFLKRLLVGGLERVFEIGRAFRN
                     EGIDTRHNPEFTILEAYQAYTDVNGMMDLTEELIRNAARKIKAVVRPAVDGAAPADPA
                     RLSFTYRGKTVALADPFARVSVEEEFKKLGLDYRAICREGNWKAAAAKVGLDVDGAPD
                     AKCFEAILDERILPKVPPASFVYGYPAVFSPLAKQ"
BASE COUNT          214 a          359 c          344 g          191 t
ORIGIN      
        1 gtcggccgcc gccgctttgt tggagcgcca caaggacctg cccgccagcg gccacgccga
       61 ggaaaccgtg gccgtgtccg gacgggtggt cacctgccgg gacatgggga aaaccatctt
      121 cgcccacctg caggatttgt ccggtaaaat ccaaatttat ttcaagaaag acgaactccc
      181 cgaggccttt ctggccttcc aaaaaaccgt ggattccggc gacatcgtcg gggtcgaggg
      241 gtcccccttc cgcacccgca cgggggaaat cacggtgcac gtcaaaagct ggcgcctcct
      301 ggccaaggcc cttcgcccgc cgcccgaaaa attccacggc atgaccgacg tggaaatccg
      361 ttatcgacga cgggaagtcg atttgttcag caacccagag gcccgggaga ggtttttgga
      421 ccgccaacgc attgtgagcg ccctccggga ggtccttctc caaaagaatt tcatcgaagt
      481 ggaaacgccc attttgcagg ccgtggcggg cggcgcggcg gccaagccct tcgtcaccca
      541 gcaccacgcc ctgggcatgg aactgttcat gcgcatcgcc ccggagctct tcctcaagcg
      601 actcctcgtg ggcgggttgg agcgggtctt tgaaatcggc cgggcgttcc gcaacgaggg
      661 cattgacacc cgccacaacc cggagttcac catcctggag gcctaccagg cctacaccga
      721 cgtgaacggc atgatggatt tgaccgagga attgatccgg aacgccgcgc ggaaaatcaa
      781 ggccgttgtc cggccggcgg tcgacggtgc cgccccggcg gaccccgccc ggctctcctt
      841 cacctaccgc ggcaagaccg tggccctggc ggaccccttc gcccgggtat ccgtcgaaga
      901 ggaatttaag aaattggggc tggactaccg ggcgatctgc cgggaaggga actggaaagc
      961 cgccgccgcg aaggtcggtt tggacgtgga cggcgccccg gacgcgaaat gcttcgaggc
     1021 cattctcgac gaacggattt tgccgaaggt gccgccggcg agtttcgttt acgggtatcc
     1081 cgccgtgttc tcgcccctgg ccaagcag
//
LOCUS       DAOQLS010000816         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_123479_length_1106_cov_4.510942, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000816 DAOQLS010000000
VERSION     DAOQLS010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123479_length_1106_cov_4.510942"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>1106)
                     /locus_tag="PK362_11745"
     CDS             complement(2..>1106)
                     /locus_tag="PK362_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75542.1"
                     /translation="PNGCDFCCTSHFFKRRHVKLLPTGKDIFDQVERYRQGDPNLVFI
                     IFDEDFLLNKTRALEFREHVMKSGWLPSIFVFSSVKAISQYTVEQILEMGIDGFWVGY
                     EGTKSGYAKQQGRPVEEIFTEFREHGITILASMIVGFDYQDHAVVAEELAGLMKLKPS
                     LAQFLIYGPVPSTPFYDRVKKADLIRDDYKNEPEKFYRRADGFTTMIKHPTLSPAQIE
                     EIQQWCFDQDFQRLGPSLFRVIESWLLGHQKLKGSPNPLMRQKAEFYAKEIRKAYPIF
                     LAGRLLGPNRVVRAWIAELQAEVHRTLGPATPLEKARSLLAFGMALWTWTTLRFNWFQ
                     HPPLTRTEYGRPVEKDSADVPAPVGALAPVLSD"
BASE COUNT          225 a          313 c          341 g          227 t
ORIGIN      
        1 gtcaatccga aaggaccggg gccagggcgc ccacgggcgc ggggacgtcc gcggaatcct
       61 tttccacggg acgaccgtat tcggtgcggg tcaggggcgg atgctgaaac caattgaacc
      121 gaagggtcgt ccaggtccac agggccatgc cgaaggccag aagggaccgg gctttttcca
      181 acggggtggc gggacccaac gtccgatgaa cctccgcttg aagttccgcg atccacgccc
      241 gaacgacccg gttggggccc aggaggcggc cggccagaaa gatcggatag gccttgcgga
      301 tttccttggc gtaaaactcc gctttttggc gcatcaacgg gttgggcgag cccttgagtt
      361 tttgatgtcc caacagccag gattcgatga cccggaaaag gctggggccc agccgttgga
      421 aatcctggtc gaagcaccat tgctggatct cctcgatctg ggccggactc aacgtcgggt
      481 gcttgatcat ggtcgtgaac ccgtcggccc gccggtagaa cttttcgggt tcgtttttgt
      541 agtcgtcgcg gatcaaatcc gcctttttga cccggtcgta gaagggcgta ctgggcacgg
      601 gaccgtatat aaggaattgg gccagggagg gtttgagctt catcaacccg gccaattcct
      661 cggccaccac ggcgtggtcc tggtagtcga agcccacgat catggaggcc aggatcgtga
      721 tgccgtgctc ccggaactcg gtaaaaatct cctccacggg ccggccctgc tgcttcgcgt
      781 atcccgactt tgtcccctcg taacccaccc aaaaaccgtc gatccccatt tccaggatct
      841 gttccacggt gtattggctg atggccttga cgctggaaaa gacgaagatg gacggcagcc
      901 agccgctttt catcacgtgc tcccggaatt ccagggcccg ggttttgttg agcaagaagt
      961 cctcgtcgaa aataatgaaa accaggttcg ggtccccttg tcggtagcgc tccacctggt
     1021 cgaaaatatc cttgccggtg gggagcaatt tgacatggcg gcgtttaaaa aagtgcgagg
     1081 tgcagcaaaa atcacagccg ttgggg
//
LOCUS       DAOQLS010000817         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_123487_length_1106_cov_4.202664, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000817 DAOQLS010000000
VERSION     DAOQLS010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123487_length_1106_cov_4.202664"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PK362_11750"
     CDS             complement(<1..699)
                     /locus_tag="PK362_11750"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNC75543.1"
                     /translation="MRRLRLLLSVSLLGVVSPAVASKGNTGAEFLKIGAGARPAALGD
                     TQAALSEGALAALWNPAALGGLSSAEISLTHNRWFEGIQDQRFVAAVPTKSAGTVALA
                     YERLAINDFLSFDAQGTNTGAVNSGDSVISLAWGRNLAQGATGGSGLYAGFAAKNINE
                     KIAGVSASGFALDFGFLFRPWGLTAQRLPWVRRSSLGFSAKNLGQGLKFDEENAPLPT
                     EYNLGAGYSQPLGGD"
     gene            complement(696..>1106)
                     /locus_tag="PK362_11755"
     CDS             complement(696..>1106)
                     /locus_tag="PK362_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75544.1"
                     /translation="LWVKVPNPTINTSSQTVTALIPHFSVYAVMGAPSFDPSESFAFP
                     VPWRPAGPNAGNAAGQTGTLAGGITFTNLPDACTIRLYTLSGGLIREINHSTGLQETW
                     DGRSADGENVASGTYVWVVSANGSKKTGKLAVIR"
BASE COUNT          185 a          321 c          409 g          191 t
ORIGIN      
        1 gtcgccgccc aggggctggc tatagccggc gcccaggttg tattccgtcg gaaggggggc
       61 gttttcctcg tcgaatttga gcccctgccc caggtttttc gcggaaaagc ccaggctgga
      121 gcggcgcacc caggggagcc gctgggcggt gagtccccag gggcggaaca gaaatccaaa
      181 atccagggcg aagcccgaag cgctgacacc ggcgattttt tcgttgatgt ttttggcggc
      241 gaacccggcg tagaggccgc tgccgccagt ggcgccctgg gcaaggttcc ggccccaggc
      301 cagggaaatc acgctgtcgc cggagttgac cgccccggtg ttggtgccct gggcgtcgaa
      361 agagaggaag tcgttgatgg ccaggcgttc gtaggccagg gccaccgtgc cggccgattt
      421 ggtcgggacg gcggccacaa accgctggtc ctgaatgcct tcaaaccaac ggttgtgggt
      481 gaggctgatt tcggcggagg aaaggccgcc cagggctgcg gggttccaga gggccgccag
      541 ggcgccttcg gacagggccg cctgggtgtc gcccagagcc gcgggccggg ctccggcgcc
      601 gatctttagg aattccgccc cggtgttccc tttggaagcc acggcggggg aaacgacgcc
      661 caagagggag acgctcaaaa ggagacgaag tctcctcatc ggatgaccgc cagtttgccg
      721 gtttttttgg agccgttggc ggacacgacc cagacatagg tgccgctggc gacgttttct
      781 ccgtcggcgc tgcgcccgtc ccaggtttcc tggaggcccg tgctgtggtt gatctcccgg
      841 atcaatccgc ccgaaagggt gtaaaggcgg atggtgcagg cgtccgggag attggtgaaa
      901 gtgatgccgc ccgccagggt gcccgtttgg ccggccgcgt tgcccgcgtt ggggcccgcg
      961 ggccgccagg gcacggggaa ggcgaaggat tccgaggggt caaaggacgg cgcccccatg
     1021 acggcgtaga cggaaaaatg gggaatgagc gccgtgacgg tctggctgga ggtgttgatc
     1081 gtcgggttcg gaactttgac ccacag
//
LOCUS       DAOQLS010000818         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_125016_length_1100_cov_1.776077, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000818 DAOQLS010000000
VERSION     DAOQLS010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125016_length_1100_cov_1.776077"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..614)
                     /locus_tag="PK362_11760"
     CDS             complement(21..614)
                     /locus_tag="PK362_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75545.1"
                     /translation="MALVLFVMGIGVLAVAEEDTVSYDERQTVDGEDADLAPGPEFSG
                     DVRVRAREVLPPGSTYEAAPPELQHNVYFYQRTRAAWGPVGAGWLAKRASVGPALSLD
                     NLREYGTLKGSVDLKETGPLRRAVAGNYTLAFGQGLLFYDGFGEYVRPVQVKDNGPRP
                     DYTSGTNDYLRGAAGRASFGPVGLDLFVSEKPLENNL"
     gene            complement(896..>1100)
                     /locus_tag="PK362_11765"
     CDS             complement(896..>1100)
                     /locus_tag="PK362_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75546.1"
                     /translation="HRMGDRVGLGATLKRQEVRMERYGSLAAYQVDAGIFGHPLPKVG
                     AGVSVKNLTQSRLGGTPESPPAL"
BASE COUNT          235 a          362 c          322 g          181 t
ORIGIN      
        1 gattcatgga ctttatcctc ttataaatta ttttccaagg gtttttcgga gacgaagagg
       61 tcgagcccca cgggcccgaa agacgcgcgg cccgccgcgc cgcgcaaata atcgttggtt
      121 cccgacgtat aatccgggcg cgggccgttg tctttcacct gcacgggccg aacgtattcc
      181 ccaaacccgt cgtagaaaag aagcccttgg ccaaacgcca gggtatagtt ccccgccacc
      241 gctcggcgga gagggccggt ttccttaaga tcaaccgatc ccttcagcgt tccatattcc
      301 cgcaggttgt cgagggaaag agccggcccc acgctggccc gtttggccaa ccaacccgcg
      361 cccacggggc cccacgccgc ccgcgtccgc tgataaaaat agacgttgtg ttggagttcc
      421 ggcggagcgg cctcgtaggt cgatcccggc gggaggacct cccgggcgcg cacgcgaaca
      481 tccccggaga attccggccc cggggcgaga tcggcgtcct ccccgtcgac ggtttgacgc
      541 tcgtcgtagc tgacggtgtc ttcctccgcc acagccagca cgccgatccc catgacaaat
      601 aagacgagcg ccaacgatcg ggaacaactc acagaaagag ggaaagatta aaggagtgtt
      661 ggtccggaag cgtcgggtgc gtgaggaacg cgtagtccaa agcaaagaac gaaaaccgca
      721 aacccatccc taaagcaaac cggttcgggc cggtttcaaa ccccgcacgg agagcgaagg
      781 cgggatggag ccaggcttct tgccccgccc gccacgacac ttttccgccg gactcggata
      841 ccacggccaa agaggtgggc ccgcgggcca aggccgtcac ggagaggcca gcgctttaaa
      901 gcgccggggg cgattcaggc gtgccaccca ggcgagactg ggtaaggttc ttcacggaca
      961 cgccggcgcc cactttggga agaggatgcc cgaaaatccc cgcgtccacc tgataggcgg
     1021 ccaaactccc ataccgttcc atccgaacct cttgtcgttt gagcgtcgct ccaagcccca
     1081 cccggtcccc catgcgatgt
//
LOCUS       DAOQLS010000819         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_125806_length_1096_cov_5.118156, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000819 DAOQLS010000000
VERSION     DAOQLS010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125806_length_1096_cov_5.118156"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PK362_11770"
     CDS             complement(<1..325)
                     /locus_tag="PK362_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75547.1"
                     /translation="MKSTVHILIPFDLGLELDFVGPDATAVAKEISSHPIRDLAFEGR
                     RFADIRLAAQIYKFGVGLLALSFAADGDLDFFAALSCRTEALQVEGAPIIPWAKTLVD
                     GVNGSA"
     gene            410..985
                     /locus_tag="PK362_11775"
     CDS             410..985
                     /locus_tag="PK362_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75548.1"
                     /translation="MKQWALVFILGALAVPGRAEDGLRIPRSSSASLATGGDESTLSW
                     PFGSVRLSSAALTRAAPETPTRGAGLAETEGTFRLKYELARTGDLVVARRLTVTAASR
                     REFHVAPSAKLREHEAVHVAINDREARRIQEALAGFRVRGLTTREAERRLKKRFREEV
                     AAVRRLHAEWDETHVFVSTGPALPEVTPGPK"
     gene            complement(956..>1096)
                     /locus_tag="PK362_11780"
     CDS             complement(956..>1096)
                     /locus_tag="PK362_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNC75549.1"
                     /translation="PTMDEAAREAYGRVKNVIIPNMFYRTDIGERWRRDGDLLRTWGY
                     LR"
BASE COUNT          187 a          351 c          372 g          186 t
ORIGIN      
        1 gggcactgcc gttcaccccg tccaccaggg ttttggccca ggggatgatg ggggcccctt
       61 cgacctgcag ggcctcggtg cggcaggaca gggccgcgaa aaaatccagg tcaccgtcgg
      121 cggcgaagga cagcgccagg aggcccaccc cgaacttgta aatctgcgcc gccaggcgga
      181 tgtcggcgaa ccgacggccc tcgaaggcca gatcgcggat gggatggctc gaaatttcct
      241 tggccacggc ggtggcgtcg gggccgacga aatccaattc aagccccagg tcgaagggga
      301 tcaaaatgtg cacggtgctt ttcatgggag tcctcgcctt taagggtagg ccatccgggc
      361 gtgtctgtca acggccgcga ccggcgatcg ctttttatag aatccgaccg tgaaacaatg
      421 ggccttggtt ttcattttgg gcgcgctggc cgtgcccggt cgcgcggagg acggactgcg
      481 gattccccga tcctcctcgg ccagccttgc caccgggggc gacgaatcga ccctgtcctg
      541 gcccttcgga tccgttcggc tttcctcggc ggccctgacc cgcgccgcgc ccgagacccc
      601 aacccgcggg gcgggcctgg cggaaaccga agggaccttc cgcctgaagt acgagttggc
      661 gcgaaccggc gacctcgtgg tggcccgtcg attgacggtg acggcggcca gccggcgcga
      721 attccacgtt gcgccttcgg ccaaactccg ggaacacgag gccgtccacg tggccatcaa
      781 cgatcgggaa gcccggcgaa ttcaagaggc cctggcgggg ttccgggttc ggggcctgac
      841 gacccgggag gcggagcgtc gacttaaaaa gagatttcgg gaggaagtgg ccgccgtccg
      901 ccggcttcac gccgagtggg acgaaaccca cgtgttcgtt tcgacgggcc ccgccttacc
      961 ggaggtaacc ccaggtccga agtaggtcgc cgtcccgacg ccagcgctcc ccgatgtcgg
     1021 tgcggtaaaa catgttggga atgatgacgt tcttgacccg tccgtaggct tcccgggccg
     1081 cttcgtccat ggtggg
//
LOCUS       DAOQLS010000820         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_126024_length_1096_cov_1.467819, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000820 DAOQLS010000000
VERSION     DAOQLS010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126024_length_1096_cov_1.467819"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..694)
                     /locus_tag="PK362_11785"
     CDS             complement(35..694)
                     /locus_tag="PK362_11785"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNC75550.1"
                     /translation="MAVRKVDEIRNPQMDYTLRCHITSFSSKDSSHASSFDVFVKGKD
                     KTFIRQTEPADEKDRLILMRERAVWTYFPSASKPIRVPLQERLTGDIAIGDIARANFT
                     NDYSARFVPPAPGEATSLYKIELLAVMEEVTYARVILWANKSNFRPVKAEYYAVSGRL
                     LKTCTFENYKMLGGAVRPSRLVMVDAVQKDKRSIVDYDQIRLGQLSESLFTKDRLKKI
                     Q"
     gene            925..>1096
                     /locus_tag="PK362_11790"
     CDS             925..>1096
                     /locus_tag="PK362_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC75551.1"
                     /translation="MTLIQAKNVTKVYSLGALSIEALRGVSVSADAGEFVALAGPSGS
                     GKTTLLNLLGCLE"
BASE COUNT          247 a          298 c          303 g          248 t
ORIGIN      
        1 cggaaagggg cggggtgttg cggagacgga tccactattg aatttttttg agacggtctt
       61 tcgtaaacag gctctccgag agttgcccca ggcggatttg gtcgtaatcc acaatcgatc
      121 ttttatcttt ctgcaccgca tcaaccatca ccaaacggga gggacgaacg gcgccgccga
      181 gcattttgta gttctcaaag gtgcaggttt tcaagagtcg gccggaaacc gcgtaatatt
      241 ccgctttcac ggggcgaaaa ttgctcttgt tggcccagag aatgaccctg gcataggtca
      301 cctcttccat cacagcgagg agctctattt tgtaaagcga ggtcgcctcc cccggcgccg
      361 gcggaacgaa ccgcgcactg taatcgttcg taaaattggc gcgggcgatg tcgccgatgg
      421 cgatgtctcc ggtcagccgt tcttgcaacg gaacgcggat gggtttcgac gcgctgggaa
      481 aataagtcca cacggcccgt tcgcgcatca gaatgaggcg atctttttcg tcggcgggct
      541 cggtttgtcg gatgaacgtt ttgtctttgc ccttcacgaa cacatcgaag ctggaggcgt
      601 gcgaactgtc tttcgagcta aaggaagtga tgtggcaacg gagagtgtaa tccatttggg
      661 gattgcgaat ttcatccacc ttccgaaccg ccatctccgc cgaaggcgat gccgcgacac
      721 caggccacga tccccacgcg accaaggcac cggcttgaac ccagcgattc catttccgaa
      781 aaggcgattc cgtcatggtg ccccctattc aaacacatgt tgaatccgtc ttcaataaat
      841 ataggcgatt tgacaataaa gtcaaggcca ttgacaaata gcccaacccc cttataatag
      901 atcgctgggt gaggaacgct ccccatgaca cttattcaag ccaagaacgt gacgaaggtt
      961 tactcgctgg gggccctctc aatagaggcc cttcgggggg tcagcgtttc ggcggatgcc
     1021 ggcgaattcg ttgccctggc tggaccttcc ggaagcggca agaccactct gctgaacctg
     1081 ttgggatgcc tggaac
//
LOCUS       DAOQLS010000821         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_126412_length_1094_cov_1.968239, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000821 DAOQLS010000000
VERSION     DAOQLS010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126412_length_1094_cov_1.968239"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1094
                     /locus_tag="PK362_11795"
     CDS             <1..>1094
                     /locus_tag="PK362_11795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC75552.1"
                     /translation="YACARLSEQKGFDFVADALEDLLTEFPNAQFVIMGDSDNSEMGN
                     DLARRFADFRARYPGRVWFGPFAGRGGWEHYMGAAADFVLSPSRFEPAGLAEKKGMRL
                     GAVPIVRAVGGLQDTVIDPRDDSDNANGFKFTEPTGAAFGEAARRALRAYQAGHGQRT
                     VDEPNIDLLQQNAMRTPKSWEDVAREYLGLYSAARDAAHTESTAEIAARRQALHPTAP
                     LIGHLPAGVDLGSIRLDRRSEISGALAELDVWLATVISGPPPTLAGSRRGESVQKTLD
                     LLREEIRTLAHYADAGLFDAGLFDADALQRHDSARVLGFGFAETKTLTGRARLGLSDE
                     FFNPKSVLYPHRREALFHEAYHVILGLADN"
BASE COUNT          183 a          358 c          347 g          206 t
ORIGIN      
        1 tgtacgcctg cgcccggctg tcggagcaaa aagggttcga tttcgtggcg gacgccttgg
       61 aagatttgct gacggagttc cccaacgccc aattcgtcat catgggcgac agcgacaatt
      121 cggaaatggg caacgacctg gcccggcgtt tcgccgattt ccgagcccgc tacccgggcc
      181 gcgtttggtt cggtcccttc gcggggcgcg gcggctggga acattacatg ggggcggccg
      241 cggattttgt tttgtccccc tcccgttttg aacccgccgg ccttgccgaa aagaagggca
      301 tgcgactggg cgcggtcccc atcgtccgcg cggtgggggg gttgcaggac accgtcatcg
      361 atccccggga cgattccgac aacgccaacg gctttaagtt cacggagccc accggcgccg
      421 ccttcggcga ggccgcgcgc cgggccctgc gggcctatca ggccggtcac ggtcagcgga
      481 cggtcgacga gccgaacatc gatctgctgc aacaaaacgc catgcgcacc ccgaaatcct
      541 gggaggacgt ggcccgggag tacctcggcc tctattcggc cgcccgggac gcggcccaca
      601 ccgaatccac ggcggaaatc gccgcccgtc ggcaagccct tcacccgacg gcccccctga
      661 tcggccattt gcccgcgggg gttgatttgg gttcgattcg tttggatcgg cgcagtgaaa
      721 tcagcggagc cctggccgag ctcgacgtgt ggttggcgac ggtgatcagc ggaccgccgc
      781 cgaccctggc gggttcccgc cgtggcgaga gcgttcaaaa aactttggat cttcttcgcg
      841 aggaaatccg tacgttggcg cactatgccg acgccggcct cttcgacgcc ggtctctttg
      901 acgccgacgc ccttcaacgc catgattcgg cccgggtgtt gggtttcggg ttcgccgaaa
      961 ccaaaacgtt aaccggtcgg gcgcgactgg gcttgagcga tgaattcttc aatccgaaat
     1021 ccgtccttta cccccatcgg cgggaagcgc tctttcacga agcctatcac gtcatcctcg
     1081 ggttggcgga caac
//
LOCUS       DAOQLS010000822         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_126542_length_1093_cov_3.018304, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000822 DAOQLS010000000
VERSION     DAOQLS010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126542_length_1093_cov_3.018304"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK362_11800"
     CDS             <1..483
                     /locus_tag="PK362_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC75553.1"
                     /translation="VLVYFQLGLVIGNLYYRMDELDKSVESYRRVLAKKPGDALVSFN
                     LAFVLRDRKNWTEALDALQPFTEGGRTLPTGGHKLLGDIFRGQGDLPKALAEYVLERS
                     ASGDSAELAQAMAQAYEDSAQDELALERWEHVAKLDPKNREARARLRRLKKRLARASR
                     "
     gene            480..>1093
                     /locus_tag="PK362_11805"
     CDS             480..>1093
                     /locus_tag="PK362_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75554.1"
                     /translation="MIRALRPLPLVLLLGAPAALPAFGQNKVRSDSFDWRIVRTPHFE
                     IHATPEGAALLPRVAQILEESHARITADAGVPVPGPTPVFLYATHNEFEQTNIADIGE
                     GTGGVTEAFKNRLLIFNDGTDAWLRHVLPHEFTHVVEFEALYGGLWRSARLLKSPFYP
                     LWFMEGLSEYESGEVDRAEEDMVLRDAATRGALYPLSELHGFNHL"
BASE COUNT          205 a          379 c          305 g          204 t
ORIGIN      
        1 gttttggtgt acttccaatt aggattggtt attggcaacc tgtattaccg gatggacgag
       61 ctggacaaaa gcgtcgaatc ctatcggcgg gtgttggcca aaaaaccggg cgacgccctg
      121 gtctccttta acctcgcttt cgtcctgcgg gaccgcaaaa attggacgga ggccctggac
      181 gctctccaac cctttaccga aggcggtcga actctgccca ccggcggtca caagctgttg
      241 ggcgacattt ttcgcggcca aggggatctg ccgaaagccc tggcggaata cgtcctggaa
      301 cgttcggcct ccggcgacag cgccgaattg gcccaggcca tggcccaggc ctacgaagat
      361 tcggcccaag acgaattggc cctggagcga tgggagcacg tcgccaagtt ggaccctaaa
      421 aaccgggaag cccgcgcccg cctgcgccgg ctcaaaaagc gcctggcgcg cgcctcccgg
      481 tgatccgggc cctccgcccg ctccccctcg tcctcctcct gggcgcgccc gccgcccttc
      541 ccgccttcgg ccaaaacaaa gtccgttcgg attcctttga ttggcggatc gttcgaacac
      601 cgcattttga aattcacgcc acccccgaag gggcggccct tcttccccgg gtcgcccaaa
      661 ttctggagga atcccacgcc cggatcacgg ccgacgcggg cgtccccgtt ccgggcccca
      721 cgcccgtttt cctctacgcc acccacaacg aattcgaaca aaccaacatc gccgacatcg
      781 gggaagggac cggcggcgtc accgaggcct ttaaaaaccg tcttttgatt ttcaacgacg
      841 gcaccgacgc ctggcttcgc cacgtcctcc cccacgaatt cacccacgtg gtcgaattcg
      901 aggccctcta cggcggcctc tggcggtcgg cgcggctact taaatcgccc ttctacccgc
      961 tttggttcat ggagggtctc tccgaatatg aaagcggcga ggtggaccgg gccgaggagg
     1021 acatggtcct gcgggacgcc gccacccggg gcgccctcta cccattaagc gaacttcacg
     1081 ggttcaacca tct
//
LOCUS       DAOQLS010000823         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_126950_length_1091_cov_4.645753, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000823 DAOQLS010000000
VERSION     DAOQLS010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126950_length_1091_cov_4.645753"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1091)
                     /locus_tag="PK362_11810"
     CDS             complement(<1..>1091)
                     /locus_tag="PK362_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNC75555.1"
                     /translation="VEGSAKVTPALQKLAEGFVAKSTSTEIRIQEVFNYIDDNIRYVS
                     MNLSQNGLTPHPAEETLKNRYGDCKDRTALAIALLKGLGVKAYPALVAPALPEIQDAL
                     PRWGYFNHMIAAVEHNGLHFVESTDNNFPYTEQHPGLNGAQVFLINGQGGRRLNLPTE
                     NVTRPVTLSKTHIQLNPDGSGRLDQTGFLARKTFPGVRQGAREIDSMPKPLAEKEKQK
                     MLAAVHAGIQEGVMELHGVDQRFGRIQAHITGAVPGLAQKNGPFLVYDLEIEDRMENF
                     PDSPRVHPLWFRETDYARTETTIQLPAGHAVDHLPRGFDMKTDGIRFKRTCKLKGNTF
                     TVIEDRWLTPARLPATEYARLRQFHQELK"
BASE COUNT          177 a          279 c          379 g          256 t
ORIGIN      
        1 ccttgagctc ttggtgaaat tggcggagcc gggcgtattc cgtggcgggg agtcgcgcgg
       61 gtgtgagcca ccggtcttcg atcacggtga aggtgttgcc cttcagctta caggtgcgtt
      121 taaagcgtat gccgtcggtt ttcatgtcga agccgcgggg aaggtggtcc acagcgtgcc
      181 ccgccgggag ctggatcgtt gtttcggtgc gggcgtagtc ggtttctcta aaccacaggg
      241 ggtggacgcg gggggagtcg gggaagtttt ccatgcggtc ttcgatttcc aagtcgtaga
      301 ccagaaaggg gccgtttttc tgggcaagtc cggggaccgc gccggtgatg tgggcctgaa
      361 tgcgcccgaa gcgttggtcc acgccatgca attccatgac gccctcttga atgcccgcgt
      421 gaacggccgc cagcattttt tgtttttctt tttccgccaa cggtttgggc atggagtcga
      481 tctcgcgagc gccttggcgc acaccgggga aggtttttcg tgcaaggaac cccgtctggt
      541 ccaaccggcc ggaaccgtcg gggttgagct gaatgtgtgt ctttgacaag gtcacggggc
      601 gggttacgtt ttcggtgggg agatttaacc gtcggccccc ttggccgttg atgagaaaga
      661 cctgcgcgcc gttgaggccg gggtgttgct cggtgtaggg gaagttgttg tccgtggatt
      721 ccacgaagtg gagcccgttg tgctccaccg cggcgatcat gtggttgaaa tacccccagc
      781 gcggaagcgc gtcttgaatc tccggcaggg cgggggccac cagggcggga taggctttga
      841 cgccaagccc tttcaagagc gcgatggcga gggccgtacg gtctttgcag tccccgtaac
      901 ggtttttgag ggtttcctcc gccgggtggg gggtgaggcc gttttggctc aggttcatag
      961 agacgtagcg aatgttgtca tcgatatagt tgaaaacctc ctgaatgcgg atttcggtag
     1021 aggtggactt ggccacgaag ccctcggcca acttttgaag ggccggcgtg actttggccg
     1081 acccctccac c
//
LOCUS       DAOQLS010000824         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_128093_length_1087_cov_2.213178, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000824 DAOQLS010000000
VERSION     DAOQLS010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128093_length_1087_cov_2.213178"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PK362_11815"
     CDS             <1..533
                     /locus_tag="PK362_11815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC75556.1"
                     /translation="SLKDGPLKVTDEDIRSFWETEKEVRAAHILVSRRDLADALRKKI
                     AAGEKFEDLAKAHSEDPASAAKGGDAGYLLPGSLVPEFENALFSMKRGETSDVVVSPY
                     GFHIIRRGDDRPLSKHPLDDAMKTRLRHALESRKLSAWFESIRQRHNVQINNENLQDV
                     VIPTPGAAPLEKANSR"
     gene            583..>1087
                     /locus_tag="PK362_11820"
     CDS             583..>1087
                     /locus_tag="PK362_11820"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNC75557.1"
                     /translation="MKKIALALAFGLTAVSPLFAAKLTNKPIAIVNGETILMSDFQKN
                     WEAFQEQQKENGKPEGMTDDFKKEARQKLFDQMVDDKVLQAEAKKRKIRVPQRDLENG
                     ILQVKARFLPDAGRKDLQTIVRRQMTAQGDNPEATPDLTAAWKELSTSNPAAVKEAEA
                     TFKSELAK"
BASE COUNT          280 a          342 c          286 g          179 t
ORIGIN      
        1 aatcgctgaa agacggcccg ctcaaggtga ccgacgagga tatacggtct ttttgggaaa
       61 ccgaaaaaga agtccgcgcc gcccacattc tggtgagccg ccgcgacctg gccgacgcgc
      121 tccgcaaaaa aatcgccgcg ggcgaaaagt tcgaggattt ggccaaggcc cattccgagg
      181 accccgcctc ggccgccaag ggcggggacg cgggctattt attgccgggc tctttggttc
      241 cggagttcga gaacgccctt ttctccatga aacgaggcga aacctccgac gtggtggtgt
      301 ctccttacgg gttccacatc atccgccggg gagacgatcg ccccctttcc aagcaccccc
      361 tggatgacgc catgaaaacc cgccttcgcc atgccttgga aagccgcaaa ctgtcggcct
      421 ggtttgagtc gattcgccag cgccacaacg ttcaaatcaa caacgaaaat ctgcaagacg
      481 tggtgatccc cacccccggg gcagctccct tggaaaaagc caattcccgt taacaatcaa
      541 tatgatagaa tcccgacacc cacttcacag gaggatttcc ccttgaaaaa aatcgccctt
      601 gcgttggcat tcggcttgac ggccgttagc cctttgttcg ccgcaaagct caccaacaaa
      661 cccatcgcca tcgtcaacgg cgaaaccatt ctgatgtccg acttccaaaa gaattgggag
      721 gccttccagg aacagcaaaa ggaaaacggg aaacccgaag ggatgacgga cgatttcaag
      781 aaagaagccc ggcaaaaatt gttcgaccag atggtggacg acaaggtcct ccaggcggag
      841 gccaagaaac gcaagatccg cgtgccccag cgggacctgg aaaacgggat cctgcaggtg
      901 aaagcgcgct ttctgcccga cgccggacgc aaagatcttc aaaccatcgt tcgacgccag
      961 atgaccgccc aaggcgacaa cccggaagcc acgccggacc tcacggccgc gtggaaagaa
     1021 ctctccacct ccaaccccgc cgccgtaaag gaggccgagg ccacctttaa aagcgaactt
     1081 gccaagg
//
LOCUS       DAOQLS010000825         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_128711_length_1084_cov_3.176871, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000825 DAOQLS010000000
VERSION     DAOQLS010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128711_length_1084_cov_3.176871"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PK362_11825"
     CDS             complement(<1..1049)
                     /locus_tag="PK362_11825"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HNC75558.1"
                     /translation="MARVSADLTVREDRRRRLEFYRKQTGTDPEDAGTGFVQGLYGFD
                     VPPFSLATDLRGNITAAGGFQWYGGMPAPGLLRLTAGGHFDETFPRFPDFPVLFSRPR
                     VLVAQSGNTYVNVGLSGGAPPTRILPNGQIDAQFAQVVGDPAHPPYSPDHMVFQSDGR
                     LVFSNEVRFVGMAPLAIVRLKPSGLIDEEFTQRANADLDARWGAARCSVVGVGSDGRI
                     FALVEKSSGAPVGRLVAFNPDGSLDATFAPPADLTIRNFRCAPDDAVYIVATPGPGTP
                     AGLMRLLENGRTDPSFGPPAYGTAIKDLEVQKDGKVLALGRLKNAVGHPAGLVRFLPD
                     GRFDPAFLPNRDSHG"
BASE COUNT          191 a          381 c          350 g          162 t
ORIGIN      
        1 atcccgtggg agtcccggtt cggcaaaaag gccgggtcga agcgcccgtc gggcagaaac
       61 cgcaccaacc ccgccgggtg gcccacggcg tttttaagcc gccccagggc cagcaccttt
      121 ccgtctttct gcacttccag gtctttgatc gccgtgccgt aggcgggagg gccaaaggac
      181 ggatccgtcc ggccgttttc caacaggcgc atcagtcccg ccggggtgcc gggcccgggc
      241 gtggccacaa tatagacggc gtcgtccggc gcgcagcgga agttccgtat ggtcaggtcc
      301 gccggcgggg cgaaggtcgc gtccaagctg ccgtcggggt tgaaggccac caatcgcccc
      361 accggcgcgc cggagctttt ctccaccagg gcgaatattc ggccgtcgct ccccactccc
      421 accaccgagc accgcgccgc gccccaccgg gcgtccaggt ccgcgttggc ccgctgggta
      481 aattcctcgt cgatcaaccc gctgggtttc agccgtacaa tcgccagggg ggccatgccc
      541 acgaagcgca cctcgttgga aaacaccagg cgaccatcgg attgaaagac catgtggtcc
      601 ggggaatagg gcgggtgcgc cgggtcgccc accacctggg cgaattgggc gtcgatctgc
      661 ccgttgggca ggatccgcgt gggcggcgct ccgccggaga gccccacgtt cacgtaggtg
      721 ttccccgact gggccaccaa cacccggggc cgggagaaaa ggaccgggaa gtccgggaac
      781 cggggaaagg tctcgtcaaa atgtcccccg gccgtcaggc gcaaaagacc cggcgcgggc
      841 attccgccgt accattggaa tcccccggcc gccgtaatgt tcccgcgcag gtccgtggcc
      901 agggaaaagg gcggcacatc gaacccgtaa agcccctgga caaacccggt gcccgcgtcc
      961 tccgggtcgg tgccggtctg ctttcggtaa aattccaatc gtcgtcgacg gtcttcccgc
     1021 acggtcaggt ccgccgacac cctcgccacg aaggccggcc cggtgtaaac cacccaaaag
     1081 gcca
//
LOCUS       DAOQLS010000826         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_129132_length_1082_cov_4.695229, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000826 DAOQLS010000000
VERSION     DAOQLS010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129132_length_1082_cov_4.695229"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..>1082
                     /locus_tag="PK362_11830"
     CDS             108..>1082
                     /locus_tag="PK362_11830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNC75559.1"
                     /translation="MTTAHKLHPLLQSLLREVSERKGSDIHIGPRFVPMIRINGDLEP
                     SATGEALTAEQTRDMGYQLMGDSLKPRFDEKNEIDMAFALSTGGRFRVNIYNQRGAVN
                     LALRYIPPQLPTIQSLGLPFAVSKLAECRRGLILISGTTGSGKSTTLAAIVDHINSTR
                     KAHILTIEDPIEFVHANKKSIVNQRELGQDTRSFADALRAAMREDPNVILVGEMRDLE
                     TVQAAITAAQTGHLVLSTIHTTDTIQIISRILDLYPPHQQAQVRLQLAETLQGAISQR
                     LLPTVDGRSREAALEILVTTPHVQKAIQDNNFGDLHNAVRNGGFYGMQT"
BASE COUNT          227 a          391 c          283 g          181 t
ORIGIN      
        1 cggaccggcg ttcgaacggc ggccgcgccc ctggacgtgc ccgtcttggc cgacgacctt
       61 cgccgcccgg cgtacctccg acgtcaactt cgaaagctcc actgaccgtg accaccgccc
      121 acaaactcca ccccctgctt caatccttgc ttcgggaggt ctccgaacga aaggggtcgg
      181 acatccacat cggtccgcgc ttcgtcccca tgatccgcat caacggggac ctggaaccct
      241 ccgccacggg cgaggcgttg accgcggaac aaacccggga catgggctac caattgatgg
      301 gggattccct gaagccccgt tttgacgaaa aaaacgaaat cgatatggct ttcgcccttt
      361 ccaccggcgg gcggtttcgg gtgaacatct acaatcaacg cggcgcggtg aatctggccc
      421 tgcggtacat tcccccgcaa ttgccgacga tccaaagcct cggtttgccc ttcgccgtct
      481 ccaaactcgc cgaatgccgt cgcgggctga tcctcatcag cggcacgacg gggtccggca
      541 agtccaccac cctggcggcc atcgtcgacc acatcaactc cacgcgcaag gcccacatat
      601 tgaccattga agaccccatc gaatttgtcc acgcgaacaa gaaatccatc gtgaaccagc
      661 gggagttggg ccaggacacc cggagttttg ccgacgccct ccgcgccgcc atgcgggagg
      721 accccaacgt catcctcgtc ggcgaaatgc gggacctgga aacggtccag gccgccatca
      781 ccgccgccca aaccggccat ctggtgctgt cgaccatcca caccaccgac accatccaaa
      841 tcatcagccg gattttggat ctgtaccccc cgcaccagca ggcccaggtg cggttgcaat
      901 tggccgaaac cctccaaggc gccatttccc aacggttgtt gcccacggtc gacgggcggt
      961 cccgggaagc cgccctggaa atcctggtca cgacgcccca cgttcaaaag gccattcaag
     1021 acaacaattt tggcgacctc cacaacgccg tccgcaacgg cgggttttac ggcatgcaaa
     1081 cc
//
LOCUS       DAOQLS010000827         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_129386_length_1081_cov_4.097466, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000827 DAOQLS010000000
VERSION     DAOQLS010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129386_length_1081_cov_4.097466"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PK362_11835"
     CDS             complement(<1..261)
                     /locus_tag="PK362_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNC75560.1"
                     /translation="MVLHVRAVTRSRGMTLVEIVIAVAILGVLAVPIARFIASLTRGV
                     TKATVQSAGGERLRPAAAALDHDLLEMNQINVSSPTALQFRVD"
     gene            complement(237..>1081)
                     /locus_tag="PK362_11840"
     CDS             complement(237..>1081)
                     /locus_tag="PK362_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75561.1"
                     /translation="LDSAVSDLNGDFNFTAPALTALLVEGHNRIYALTAKGSGFSPYV
                     IKDLILDGTPPVFSAATPTGTVKTRSPYVGLLFKDATTATTTVSGVSADVLTFVAAGA
                     TVAFRYDAANGKLAWQDASTYLPPTLADGDDVTVRVEGGDNAHYKSTQTWTFHVDIDD
                     TDDTPPVVSARQPLGAGASPLPTISCRLADPESGVVPQSLVLTVDGTVVLSTATARPL
                     GLGYDAETGTLSYTPAAPYAAGSFHTVRVHAEHWAETPADPAHRALDAATNPSAEWSF
                     TCAP"
BASE COUNT          172 a          323 c          409 g          177 t
ORIGIN      
        1 gtccacccgg aactgaaggg cggtcgggct cgacacgttg atttggttca tttcgagcag
       61 atcgtggtcc aaggccgccg cggccgggcg cagccgttcg ccgccggcgg attgcaccgt
      121 ggctttcgtg accccccgag tcaaggaggc gatgaaacgg gcgatcggca cggcgaggac
      181 gccgaggatc gccaccgcga tgacgatttc caccaaggtc atgccgcggg agcgggtcac
      241 ggcgcgcacg tgaaggacca ttcggcggag gggttcgtgg cggcgtccag agcgcgatgg
      301 gcgggatcgg ccggcgtttc ggcccaatgc tccgcgtgga cgcgcacggt gtggaaagaa
      361 cccgcggcat acggcgccgc gggggtgtag gacagcgtgc cggtctccgc gtcgtagccc
      421 agcccgagcg gccgggcggt cgcggtgctc aggacgaccg tgccgtccac cgtcagcacg
      481 aggctttggg ggacgacgcc gctttccggg tcggccaatc ggcaggaaat cgtcggcagc
      541 ggggaggcgc cggcccccag gggttgtcgc gccgagacga ccgggggcgt gtcgtcggtg
      601 tcgtcgatgt cgacgtgaaa cgtccacgtt tgggtcgatt tataatgggc gttgtcgccc
      661 ccttccaccc gcaccgtcac gtcgtccccg tccgcgaggg tggggggaag gtaggtgctg
      721 gcgtcttgcc aggccaattt gccgttggcg gcgtcgtagc ggaaggcgac cgtggccccc
      781 gcggccacga aagtcaggac gtcggccgag acgccgctca cggtcgtcgt cgcggtggtc
      841 gcgtctttaa agaggaggcc gacgtagggg gagcgggttt tgaccgtccc cgtgggtgtg
      901 gcggccgaaa agaccggcgg cgttccgtcc agaattaaat ccttgatgac gtagggggaa
      961 aatccggatc ccttggcggt cagggcgtat atccggttgt gcccttcgac caacagcgcg
     1021 gtgagggccg gggccgtgaa attgaaatcc ccgttcagat ccgagacggc cgaatccagg
     1081 g
//
LOCUS       DAOQLS010000828         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_129441_length_1081_cov_2.978558, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000828 DAOQLS010000000
VERSION     DAOQLS010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129441_length_1081_cov_2.978558"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1081)
                     /locus_tag="PK362_11845"
     CDS             complement(<1..>1081)
                     /locus_tag="PK362_11845"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75562.1"
                     /translation="AVPPGDGDAWRDRLARLWGADRAATLKPVDARGANMSVTGWVSD
                     VLRPQPTGRYQRFFINRRPVVNRRLSHALYDAYRGRLPVGQHPAAVLVLQIDPEFVDV
                     NVHPTKKDVRLSHEDQVHGFLNRAVQEALARDVQTARAFGPGPDRPDAAPSRPPPFAF
                     PRPPSMTETRAAFEIQAPLTPAPPVAESPGAEGGFSRDWFRAISFAPLAQIYATYLLA
                     QAEGEFFIFDQHAAAERALYERLAADAETGARQGLLLPWVWEPAGETAALVAEQRPAF
                     ESLGFRLEAFGAASFRVTAVPAALGDSPRVRDVLEGLAEDLAAGKIPRGLEALRVRAA
                     CRGSLMAGAVLTPPEMAEVIARLQR"
BASE COUNT          173 a          357 c          408 g          143 t
ORIGIN      
        1 caccgctgaa gccgggcgat gacctccgcc atttcgggcg gggtcaggac ggcccccgcc
       61 atcaaggacc cccggcaggc cgcgcgcacg cgaagggctt cgagtccccg gggaatcttc
      121 ccggcggcga ggtcctcggc caatccctcc aacacgtccc gcacccgggg cgaatccccc
      181 aaggcggcgg gcacggccgt gacccggaag gacgcggcgc cgaacgcttc cagccggaat
      241 cccagggatt caaaggcggg gcgttgctcc gcgaccaggg ccgccgtctc ccccgccggt
      301 tcccagaccc agggaagaag cagtccctga cgggcgccgg tttcggcgtc ggcggccaac
      361 cgttcgtaca gggcccgttc ggcggccgcg tgctggtcaa agataaagaa ttccccctcg
      421 gcctgggcca acaagtaggt ggcgtagatt tgggccaggg gggcgaaaga aatagcgcgg
      481 aaccaatccc tcgaaaatcc cccctcggcc ccgggggact cggccacggg cggcgccggg
      541 gtcaacggcg cctgaatttc aaaagcggcc cgggtttcgg tcatggaggg cggacgggga
      601 aaggcgaagg ggggcggccg ggagggcgcg gcgtccggtc ggtccggccc gggaccaaaa
      661 gcccgggcgg tttggacgtc ccgggcgagc gcctcctgga cggcgcggtt caaaaacccg
      721 tgcacctggt cttcgtggga caggcgcacg tcttttttgg tcgggtggac gttcacgtcg
      781 acgaattcgg gatcgatctg gaggaccagc accgcggccg ggtgttggcc gaccggtagc
      841 cggccccggt aggcgtcgta tagggcgtgg gagagccggc ggttgacgac ggggcgccgg
      901 ttgatgaaaa accgttggta acggccggtg ggctggggcc gtagaacgtc ggaaacccat
      961 ccggtcacgc tcatgttggc gccccgggcg tcgacgggct tgagcgtcgc ggcccggtcg
     1021 gccccccaga ggcgggccag gcggtcgcgc cacgcgtcgc cgtcgccggg cggaacggcc
     1081 g
//
LOCUS       DAOQLS010000829         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_129710_length_1080_cov_2.878049, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000829 DAOQLS010000000
VERSION     DAOQLS010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129710_length_1080_cov_2.878049"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK362_11850"
     CDS             complement(<1..427)
                     /locus_tag="PK362_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75563.1"
                     /translation="MIALRYPVRVGELSYQNTVPFRLDGRWNTMPCLSPRLLARWAEE
                     GSIDAGVIPVVEAWRLEKDFEPVGQLGIAARNRVLSVVLYSRKPWIELDRGRIGLTDQ
                     TATSARLLQTLLTVRDGLRPSFREGFRPTDDARLLIGESA"
     gene            complement(424..>1080)
                     /locus_tag="PK362_11855"
     CDS             complement(424..>1080)
                     /locus_tag="PK362_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNC75564.1"
                     /translation="DQSFVLQRLKDEGLVCLPGGGAEVFSERVRKQLFPFKIGWSKWS
                     EVHRAAHGLGIRSNATMLYGHIETIDERLDHMLKLRALQDETGGFLSFIPLAFQPGNT
                     GIVERQTSVIDDLKTIAVSRLMLDNFDHIKSYWVTVGEETTSMALRFGADDIDGTILE
                     ERIMHAAKAATPVGMARERLLRLIREAGGLPVERDALYNVVREYPRSAVPDPVPAGGR
                     "
BASE COUNT          198 a          320 c          371 g          191 t
ORIGIN      
        1 aggccgattc accgatgaga aggcgggcat cgtcggtggg gcggaagcct tcccgaaagc
       61 tcggccgaag accgtcccgg acggtcagca gcgtttgaag aagccgggcc gaggtggccg
      121 tttggtccgt gaggccgatg cggccgcggt ccagttcgat ccagggtttt cgggaataca
      181 gaacgacgct caagacccga ttgcgggcgg cgatgcccaa ttggcccacc ggctcgaaat
      241 ccttttccaa ccgccaggcc tccaccacgg gaatgacgcc ggcgtcgatg gagccctctt
      301 cggcccaccg cgccaacagg cggggcgaca aacagggcat ggtgttccac cggccgtcca
      361 aacgaaaggg caccgtgttt tgataggaga gttccccgac gcgaacgggg tagcggaggg
      421 cgatcatcgg ccccccgccg gcacggggtc cgggaccgcg gaacgggggt attcgcgaac
      481 cacgttgtaa agggcgtccc gttccacggg gaggccgccg gcctcgcgga tcaggcgcag
      541 gaggcgttcc cgggccattc ccacgggggt cgccgccttg gccgcgtgca taatgcgttc
      601 ctccagaatg gttccatcga tgtcgtcggc gccgaaccga agggccatgg aggtggtttc
      661 ctcgccgacc gtcacccaat aggatttgat gtggtcaaag ttgtccagca tcaaccggct
      721 gacagcgatg gttttgaggt cgtcgatcac ggaggtttgg cgttcgacga tgccggtgtt
      781 cccgggctgg aaagccaggg ggatgaagga gaggaatccg cccgtttcgt cctggagggc
      841 gcgcagtttg agcatgtggt ccagccgttc gtcgatcgtt tcgatgtggc cgtacaacat
      901 cgtcgcgttg gagcggattc cgaggccgtg ggcggcgcga tggacctcgg accatttgga
      961 ccaaccgatc ttgaagggga aaagctgctt tcggacgcgc tcgctgaaaa cttcggcccc
     1021 gccccccgga aggcagacca atccttcgtc cttcagtcgc tggaggacga aggattggtc
//
LOCUS       DAOQLS010000830         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_130151_length_1078_cov_4.093842, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000830 DAOQLS010000000
VERSION     DAOQLS010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130151_length_1078_cov_4.093842"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PK362_11860"
     CDS             <1..1065
                     /locus_tag="PK362_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNC75565.1"
                     /translation="LDGGVGALAVASGQSAISLALLNIAQAGDEIVSADNLYGGTYNL
                     FHYTFAKFGITVKFVKSNDLDAFRKAVTPKTKAIYAESIGNPKLDVADLEGISKIAHD
                     NGLPFVLDNTVSPYLLRPFDHGVDIAVYSATKFIGGHGTSIGGLIVDSGKFNWTNGKF
                     PLIAGPDPSYHGINFVEALKPLGNIAYIIKARVTLLRDIGPALSPFNAFLLLQGFETL
                     HLRMPRHAENALAVAQFLKKHGKVAWVNYPGLADSPEQARAKKYLPKGAGAILGFGIK
                     GGKEAGRKFIDGLKLVSHLANVGDAKTLAIHPATTTHQQLSAEEQKATGVTEDFVRLS
                     VGLEHIDDILADIDQALKKA"
BASE COUNT          229 a          361 c          294 g          194 t
ORIGIN      
        1 ctggacggcg gcgtgggggc cctggcggtc gccagcggcc agtcggccat cagcctggcg
       61 ctcctcaaca tcgcccaggc gggggacgag atcgtctcgg cggacaactt gtacggtggg
      121 acgtacaatt tgttccatta cacattcgcg aaattcggga tcaccgttaa attcgtaaag
      181 tccaacgatc tggacgcctt ccgcaaagcc gtcacgccca aaaccaaggc gatttacgcc
      241 gaatccatcg gtaaccccaa gctcgacgtc gccgacttgg aaggcatttc caaaatcgcc
      301 cacgacaacg gcctgccctt cgtcctggac aataccgtgt cgccctatct tctgcgtccc
      361 ttcgaccacg gggtggacat cgccgtctat tccgcgacca agttcatcgg cggccacgga
      421 acctccatcg gggggttgat tgtggactcc ggcaagttca actggaccaa cgggaaattc
      481 ccgttgatcg ccggtccgga cccgtcctac cacggcatca acttcgtcga agccttgaag
      541 cccctgggga acatcgccta catcatcaag gcccgcgtga cgcttttgcg cgacatcggt
      601 ccggcgcttt cgcctttcaa cgccttcctg cttttgcagg ggtttgaaac cctccatctt
      661 cgcatgcccc gtcacgcgga aaacgccctg gccgtggccc aattcctgaa gaaacacggc
      721 aaagtggcct gggtcaacta ccccggcctc gccgacagcc ccgagcaggc ccgggcgaaa
      781 aaatatctgc ccaagggcgc gggagccatt ctgggcttcg gcatcaaggg cggcaaggag
      841 gcgggccgga aattcatcga cggattgaaa ctcgtcagcc acctggccaa cgtgggcgac
      901 gccaaaaccc tggccatcca tccggccacg accacccatc agcaactttc cgccgaagag
      961 caaaaggcca cgggcgtcac cgaggacttt gtgcgccttt ccgtgggcct ggaacacatc
     1021 gacgacatcc tggccgacat cgaccaggcg ttgaaaaaag cttaagacgc gccgttcc
//
LOCUS       DAOQLS010000831         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_130221_length_1078_cov_2.717498, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000831 DAOQLS010000000
VERSION     DAOQLS010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130221_length_1078_cov_2.717498"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..988
                     /locus_tag="PK362_11865"
     CDS             101..988
                     /locus_tag="PK362_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC75566.1"
                     /translation="MSQEISRRNLFKGALVAVATAVLPRSMAQASGLVSKADAFAVLV
                     DLTRCIGCRSCMRGCQRANDLTAPQELMDLDHLSTRLSPDFRQWTVVNTEGSGSTLRH
                     VKRQCMHCLEPACVSVCPVAALDQTEQGPVIYRESRCIGCRYCMMACPFDVPRFEWNN
                     GLTPVIGKCQFCAQERLFKGLLPGCVESCPTGALKFGKREDILFEAKARIRSRPDFYM
                     DHVFGEEEVGGTSWLYLSDVPFDQLGFKKNLSKRPLPSLTWDVVSLIPGIAAVLASLF
                     GVLAHSLSRKNGHGKGQGN"
BASE COUNT          195 a          318 c          322 g          243 t
ORIGIN      
        1 cccggtccag catttggttg gtggcggttt tgggactatt ggtcgggctg ggcctcggtt
       61 ggttggccgc ctggtacgcc ctgagaacta aacaaagaaa atgagccagg agatctcccg
      121 ccgaaatcta tttaagggag ccctcgtcgc tgttgccacc gctgttcttc cccgatccat
      181 ggcccaggcc agcgggctgg tttccaaggc ggacgcgttc gctgttctcg ttgacctcac
      241 gcggtgcatc gggtgtcgat cgtgcatgcg cgggtgccaa cgggccaacg atctcaccgc
      301 tcctcaagaa ttaatggatt tggatcacct gtccacccgc ctctcccccg attttcgtca
      361 atggaccgtg gtgaacacgg aagggagcgg gtccaccctc cgacacgtca aacgccagtg
      421 catgcattgc ctcgagcccg cctgcgtatc ggtgtgtccc gtggcggccc tcgaccaaac
      481 cgaacagggg ccggtgatct accgggaaag ccggtgcatc gggtgccgct attgcatgat
      541 ggcctgcccg ttcgacgttc cccgtttcga gtggaacaac ggattgaccc cggtgatcgg
      601 caaatgccag ttttgcgccc aggaacgact gtttaagggg ttgcttcccg gctgtgtgga
      661 gtcctgtccg acgggggcgc tcaaattcgg caagcgggaa gacatccttt ttgaagccaa
      721 agcacgcatt cgatcgcggc cggattttta tatggaccac gtgttcggcg aagaagaagt
      781 gggggggacc tcctggctgt atctttccga cgtcccgttt gaccaactgg ggttcaaaaa
      841 gaatctgtcc aaacgcccct tgccttccct cacctgggac gtggtcagtt taattcccgg
      901 catcgcggcg gtcctggcgt cgctcttcgg tgttctggcc cacagcctct cccgaaagaa
      961 tggccacggg aaaggccagg ggaattgatg caaataatcg gattcctatt tctagcggcg
     1021 gtctgtggtc tcttgggttg gctggccgtg gcctatgggc tcaaccgccc tcccaaac
//
LOCUS       DAOQLS010000832         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_130382_length_1077_cov_5.322896, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000832 DAOQLS010000000
VERSION     DAOQLS010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130382_length_1077_cov_5.322896"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..>1077)
                     /locus_tag="PK362_11870"
     CDS             complement(4..>1077)
                     /locus_tag="PK362_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNC75567.1"
                     /translation="ANATGCSSIYGGNLPTTPWSKSQRGFGPAWSNSLFEDAAEFGYG
                     MTLSYAKKVGYARNLVTSLRDSIGAELADSVLNAAQVTDAQVEEQRVRVNALLEKLKS
                     LPASPKVKDLQNLAENLVRKSLWIVGGDGWAYDIGYAGLDHVLASGENVNIMIMDTEV
                     YSNTGGQSSKATPFGAIAKFASGGKGKPKKDLGLIAMTYQNIYVAQVAMGANDNQCLK
                     AFLEAEQYDGPSLIIAYSPCIEHGYDLSKSVGQTKTAVESGYWPLFRYDPRLAKQGKN
                     PFQLDSKKTLPLRTHLLGENRFAPLTKGKDDATLQSMEHAQKAIDDRWAFYERLAQQP
                     APNAAAVNSAEALPAMAAAVVTK"
BASE COUNT          161 a          313 c          371 g          232 t
ORIGIN      
        1 gtcttacttg gtgaccaccg ccgcggccat ggccggcaat gcttccgcgc tgttgacggc
       61 ggccgcgttg ggcgccggct gctgggccag ccgctcgtaa aaggcccatc ggtcatcgat
      121 cgccttttga gcgtgctcca tggattggag ggtggcgtcg tccttgccct tggtgagggg
      181 ggcgaagcgg ttttcaccca gcaaatgggt ccgcagaggc agggtctttt tgctgtccaa
      241 ctggaaggga ttcttcccct gcttggccag gcgggggtcg tagcggaaca ggggccagta
      301 gcccgattcc acagcggttt tggtctgccc cacgctcttg ctcaggtcgt agccgtgctc
      361 gatgcagggg ctgtaggcga tgatgaggga cggcccgtcg tattgctcgg cttccaggaa
      421 ggccttcagg cactggttgt cgttggcccc catggccacc tgggccacgt aaatgttctg
      481 gtaggtcatg gcgatcaggc ccaggtcttt tttgggcttg cctttgccgc ccgaggcgaa
      541 tttggcaatg gcgccgaagg gcgtggcctt ggacgattgg ccgccggtgt tcgaatacac
      601 ctcggtgtcc atgatcatga tgttgacgtt ttcgccgctg gccaggacgt ggtccagccc
      661 ggcgtagccg atgtcgtagg cccaaccgtc gccgcccacg atccagaggc tcttgcggac
      721 caggttctcc gccaggtttt gaaggtcttt caccttcggc gaagccggga gggatttcaa
      781 tttttcgagc agggcgttaa cgcggacccg ttgctcctcc acctgggcgt cggtcacctg
      841 cgcggcgttc aacacgctgt cggccaactc ggccccgatg gaatcccgca gggacgtcac
      901 gaggttccgg gcgtagccga cctttttggc gtaggagagc gtcataccgt aaccgaattc
      961 ggccgcgtcc tcgaagaggg agttggacca ggccgggccg aacccgcgct gggatttgga
     1021 ccagggcgtg gtgggcaggt tgccgccgta gatggaggag cagccggtgg cgttggc
//
LOCUS       DAOQLS010000833         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_130451_length_1077_cov_2.998043, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000833 DAOQLS010000000
VERSION     DAOQLS010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130451_length_1077_cov_2.998043"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PK362_11875"
     CDS             complement(<1..1064)
                     /locus_tag="PK362_11875"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNC75568.1"
                     /translation="MTLQPAVTVMGVDGAGWVNALYDDDDDGPHPPVRVTPSSVTWVS
                     SLPGVVQMNAVKKGNYDALAEGVSDVSGTYLGLTATATVRVAGRVISSSIVTPADGRT
                     RTYLVFRPSGYNPAVPHPLILSFHGGGGSAAIQMSQSQILPLAERETILVAYPDGTGA
                     VQTWNGGACCGYASSNTVDDVGFVRALVARLKTDYAVDPARIYSTGMSNGGILSHRLA
                     CEATDLFAAIAPVAGGLNLGGDFSACRPSRQIPVLHFHGTTDDNYPYLGGVGNGLAGV
                     SFYPIPDTMDDWLRLNGIPGNARVIYQKGIETCAVHTGFPNPVQVGLCLADPPFHLLI
                     GSATYDGGGHAWPGGIQPSAT"
BASE COUNT          214 a          327 c          374 g          162 t
ORIGIN      
        1 gtggccgagg gctgaatccc gccgggccag gcgtggccgc ccccgtcgta ggtggcgctc
       61 ccgatcagga ggtggaaggg cggatcggcc aggcaaaggc ccacttggac cgggttggga
      121 aagcccgtat gcaccgcgca ggtttcgatg cctttttgat agatcacccg ggcgtttccg
      181 gggatgccgt tcaaacgcaa ccaatcgtcc atggtgtccg gaatgggata aaaggaaacg
      241 ccggccaagc cgttccccac gccgcccaag tagggataat tatcgtcagt ggtcccatgg
      301 aaatggagca ccggtatctg gcgagaggga cggcaggccg aaaaatcgcc tccgagattg
      361 agaccgccgg cgaccggggc gatggcggca aaaaggtccg tcgcctcgca agccaggcgg
      421 tgggaaagga tgcccccgtt ggacatgccc gtggagtaaa tccgggccgg atccacggcg
      481 tagtcggttt taaggcgggc gaccagggcg cgcacgaacc ccacgtcgtc gaccgtgttg
      541 ctcgacgcgt aaccgcagca cgccccgccg ttccaggttt ggacggcccc cgtcccatcg
      601 gggtaagcga ccaaaatcgt ctctcgctcg gcgaggggaa gaatctggga ctggctcatc
      661 tgaatggccg ccgagccgcc gcccccgtga aaggaaagaa tcaacggatg gggaaccgcg
      721 gggttgtatc ccgaaggtcg gaaaaccagg tacgtgcggg ttcgcccgtc ggcgggggta
      781 acgatggagc tcgagatcac ccgacccgcc acgcgcacgg tggccgtggc ggtcaaaccc
      841 aggtaggttc ccgaaacgtc ggaaacccct tcggccaggg cgtcgtaatt gcctttttta
      901 accgcgttca tctggacaac gccgggaagg gaggagaccc aggtcacgga ggagggggtc
      961 acccgaacgg gcgggtgggg cccgtcgtca tcgtcgtcgt aaagcgcgtt cacccagccg
     1021 gccccgtcca cccccatgac ggtgacggcg ggctgcaggg tcaaacgggc ggcgcga
//
LOCUS       DAOQLS010000834         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_130648_length_1076_cov_4.136141, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000834 DAOQLS010000000
VERSION     DAOQLS010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130648_length_1076_cov_4.136141"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..>1076)
                     /gene="mltG"
                     /locus_tag="PK362_11880"
     CDS             complement(122..>1076)
                     /gene="mltG"
                     /locus_tag="PK362_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNC75569.1"
                     /translation="NSAPSRVDGGLLEIPAGAGAARVARELKDRGRLRSATWFRLLSR
                     LSRADGRLKAGVYRLAPGRSAAGILKDLVAGRGEAVRLTWPEGWALWQMADRVAAAGV
                     CGREAFLSAAQGSEGFLFPETYLFEPGAPASDVVRTARDRFGSVWEEVFRAAQAAGVV
                     PPGPVPRDPDEEFRLADGRRWTQRRTVILASLVEREARRPSDRPLVAAVYHNRLKKGM
                     RLECDPTVQYALGEWKNPLWKKDLALEHPYNTYRRFGLPPGPICSPGRDALAAALAPA
                     AVDYLYFVADDTGGHSFSSNYEDHQRAVRARRRAMRAVKKS"
BASE COUNT          203 a          376 c          332 g          165 t
ORIGIN      
        1 gtcggcggcg cccacggggg ggtcttcgtt gtaatccgcc ctcaacgccg gggcgcgtaa
       61 gcccaaaagc aagaggaaca acgtttttct cagggggtct ctaaagaaca tccctcggat
      121 tttatgattt tttgacggcc cgcatggccc gtcgtcgggc ccggaccgcc cgctgatggt
      181 cttcgtaatt cgacgaaaag gaatggccgc ccgtatcgtc ggcgacaaaa taaagataat
      241 ccaccgccgc cggagccagg gccgcggcca gggcgtcgcg gccggggctg cagatgggac
      301 cgggcgggag accgaaccgt cggtaggtgt tgtacggatg ttccaaggcg aggtcttttt
      361 tccaaagggg atttttccat tcgcccaagg cgtattgaac cgtcggatcg cattccaacc
      421 gcatgccctt tttaagtcgg ttgtggtaaa cggccgccac caggggtcgg tcgctcggac
      481 ggcgggcctc ccgttccacc aaggacgcca ggatcaccgt ccgccgttgc gtccaacggc
      541 ggccgtcggc caggcgaaat tcctcatccg ggtcccgggg gaccggtccg ggcggcacaa
      601 cgcccgcggc ctgggccgcc cgaaaaacct cctcccaaac cgacccgaaa cggtcccgcg
      661 cggttcgaac cacgtcggag gccggagccc cgggctcgaa caaatacgtt tccgggaaca
      721 aaaatccctc cgatccctgg gccgcggaca gaaacgcctc gcggccgcac acgccggccg
      781 ccgcgacccg atccgccatc tgccagagag cccatccttc cggccaggtg agccggacgg
      841 cctcgccccg gcccgccacc aaatctttca agattcccgc ggcggaacgt cccggcgcca
      901 gacggtaaac accggctttc aatcgaccgt cggcccggga aagccggctc aaaaggcgaa
      961 accacgtcgc ggaacgtaga cggccccggt ccttgagttc ccgggccacc cgggccgccc
     1021 ccgccccggc gggaatttcc aacagccccc cgtcaacgcg ggagggcgcg ctattg
//
LOCUS       DAOQLS010000835         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_130653_length_1076_cov_3.983350, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000835 DAOQLS010000000
VERSION     DAOQLS010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130653_length_1076_cov_3.983350"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PK362_11885"
     CDS             complement(<1..969)
                     /locus_tag="PK362_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNC75570.1"
                     /translation="MSQPAEKLESPGSSTGPAPRPVRVLVMDPSATGRRSLADLLQRH
                     GGIRVQTLAGPAEWDPLRRDFDPQVLVLTMAAAAGHGSRFLESLLETDPLPVVLCGGD
                     DEGDSVLRCLDRGAVAALPSPSTGSGDDLSDAPTLLIGEIHRAATAALAYDCFKKRTA
                     SEALPRDLWKAGAKSGAAPIIVMAAGEGGTSALQEILKALPEDAPGVMVATRLPQSQT
                     GAFARRLAGLCRLEVKEAVPNDVLRPGQVLIAPGNRHALLAASIKAPHVEIVEGPLVG
                     GQRPSFDVLFRSAALAAGSRAVGVLLTGLGEDGVAGLAVLRAGGART"
BASE COUNT          185 a          380 c          371 g          140 t
ORIGIN      
        1 cgtgcgggcc ccgcccgccc gaaggaccgc caaccccgcc acgccgtcct cgcccaggcc
       61 cgtcaggagc acccccacgg cccgggaacc ggccgccaag gcggcggatc ggaacaaaac
      121 gtcaaagctg ggccgttgtc cgcccacgag gggcccttcg acgatttcca cgtggggggc
      181 cttgatcgac gcggccagaa gggcgtgccg gttgccgggg gcgatcaaca cctgcccggg
      241 ccgaaggacg tcgttgggaa cggcttcctt gacctccagt cggcaaaggc cggccaggcg
      301 gcgggcgaag gcgccggttt gggattgagg caaccgggtg gcgaccatca cgccgggggc
      361 gtcttccggc agcgccttta atatttcttg aagggccgag gtgccgcctt ccccggccgc
      421 catcacgatg atcggcgccg ccccggactt cgccccggcc ttccaaagat cccggggcaa
      481 ggcctccgac gccgtccgtt tcttaaaaca atcgtaagcg agggccgcgg tcgccgctcg
      541 gtgaatttcg ccgatcagga gcgtcggggc gtcgcttaaa tcgtcgcccg acccggtgga
      601 aggcgagggc aaggcggcca cggccccccg gtccaagcag cggaggaccg aatcgccctc
      661 gtcgtcgccc ccgcagagaa ccacgggaag gggatccgtt tccagaagcg attccaaaaa
      721 acgggaaccg tggcccgcgg cggcggccat ggtgaggacc aatacctggg gatcgaaatc
      781 ccgccgcagg ggatcccact cggcgggacc cgcgagggtc tggacccgga tccccccgtg
      841 ccgctgaaga aggtcggcca aacttcggcg ccccgtggcg gaggggtcca tgaccaggac
      901 ccgcacgggg cgcggcgcgg gccccgtcga cgacccgggg gattccaatt tttcagcggg
      961 ctgggacacg cgtcccccct tggccggaat ccccgaattg atcgacgtga aaaacgacat
     1021 cgcttcgcca gggcggccgg gggctgacgg cccaccgggc ccgcacgacg cttccg
//
LOCUS       DAOQLS010000836         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_131212_length_1074_cov_2.654563, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000836 DAOQLS010000000
VERSION     DAOQLS010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131212_length_1074_cov_2.654563"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PK362_11890"
     CDS             <1..522
                     /locus_tag="PK362_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75571.1"
                     /translation="PLAKSRALAQALAVSRSRDELAARRRLVDPAGMADLNGNTSTAL
                     EQDLNAVIGGLRVAIDAESVKDSRLKDKITEKVTSLGFAVADGAAGALRLKATLAVEP
                     FDRGHPQWKFYQWKGNVQLIDADGKVLGGSTPSGQEGQLIDETARAKTTEAGEDGLAQ
                     EAGRLLSQYVFGQ"
     gene            554..>1074
                     /locus_tag="PK362_11895"
     CDS             554..>1074
                     /locus_tag="PK362_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNC75572.1"
                     /translation="MKRALTWAGVAVAGLALFACGPTRPDWIMKGSGAFKKDSKVFYG
                     VGIAEGIHSESLRRQTADNRAIAEISKQLSVVSTNLMRDYMSNTSVPADQKANEEQFI
                     ENTIKTFTSNVVSGVKIIDRYQDKSGALYSLASINLEDLKTLAGEAKGLSAEVRQHIK
                     ANAEKAFDKMSEE"
BASE COUNT          229 a          339 c          340 g          166 t
ORIGIN      
        1 cccctggcga agtcccgggc gctggcccag gccctcgcgg tgtcccggtc ccgggacgaa
       61 ttggccgccc ggcggcgcct ggtcgacccc gcgggcatgg cggacttgaa cggaaacacc
      121 tccaccgccc tggaacagga tttgaacgcc gtgatcggcg gcctgcgggt cgcgatcgac
      181 gccgaatccg tcaaggattc ccgcctgaag gacaaaatca ccgaaaaagt gacgtccctc
      241 ggtttcgccg tggccgacgg cgccgccggg gccctgcgcc tcaaggcgac cctggccgtc
      301 gaacccttcg accggggcca tccccagtgg aagttctacc agtggaaagg caacgttcag
      361 ctgatcgacg ccgacggcaa ggtattgggc gggtccaccc cgtcgggcca ggaaggccag
      421 ttgatcgacg agacggcccg ggccaagacc accgaagccg gcgaggacgg gttggcccag
      481 gaagccgggc gcctgttgtc ccaatacgtt tttgggcagt aagctttttt attctttgtt
      541 caggaggatc aagatgaaac gcgcgttgac atgggcgggg gtcgcggtgg cggggctggc
      601 gttgttcgcc tgcggaccga cacggccgga ctggattatg aaggggagcg gcgccttcaa
      661 aaaagacagc aaagtgtttt acggcgtcgg catcgccgag ggcatccatt ccgaatccct
      721 ccgccgccag acggcggaca accgcgccat cgcggaaatc tccaagcagc tctcggtggt
      781 ctccacgaac ctcatgcggg actacatgtc caacaccagc gtcccggcgg accagaaggc
      841 caacgaggaa cagttcatcg aaaacacgat caagaccttc acctccaacg tcgtgagcgg
      901 ggtcaagatc atcgaccgtt accaggacaa gagcggcgcc ttgtattccc tggcgtccat
      961 caacctggaa gacctgaaaa ccctggccgg cgaggccaag ggtctctccg cggaagtccg
     1021 tcagcacatc aaggcgaacg ccgaaaaagc cttcgacaag atgagcgagg aaga
//
LOCUS       DAOQLS010000837         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_132161_length_1070_cov_4.079803, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000837 DAOQLS010000000
VERSION     DAOQLS010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132161_length_1070_cov_4.079803"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PK362_11900"
     CDS             complement(<1..701)
                     /locus_tag="PK362_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75573.1"
                     /translation="MNKWGKWATGLWAAGLAICPSALLANENDIFGYLQFNIDRTYRN
                     PNPNVATGQITRDRPDPVDYRVLRLNLMVRSTWDQGFTTFLNLSESSNASSGSSPTFI
                     VRNAWGELPLPLSLGYLRVGKIYRPFSPYNERLDAVPTYLGVEPPEIFDTDHLFMSRF
                     CDVMVHNSANLGPTFWSFAMTTNNPESRLKNGNGDSMGSFPLGWDVKMEMGNHKFMLG
                     HSGFLSGPTGPATALG"
BASE COUNT          212 a          315 c          334 g          209 t
ORIGIN      
        1 cccagggccg tggccgggcc cgtcggaccc gacaaaaagc ccgagtgacc gagcatgaac
       61 ttgtggttgc ccatttccat tttgacgtcc cacccgaggg gaaaggagcc catggagtcc
      121 ccgttcccgt ttttcagccg ggattccggg ttgttcgtgg tcatggcgaa ggaccaaaag
      181 gtggggccga gattggccga gttatggacc atcacgtcgc aaaagcggct catgaacaag
      241 tggtccgtgt cgaaaatttc gggcggttcg acgcccagat aggtgggcac cgcgtccaac
      301 cgttcgttgt agggcgaaaa gggccggtag attttaccga cgcgcaaata ccccagggac
      361 aacggcaagg gcaattcgcc ccaggcgttt cggacgataa aggtggggct cgagccgctg
      421 gaggcgttgg aggattcgga gaggtttaaa aaggtcgtga atccctggtc ccaggtgctc
      481 cgcaccatca aattaagtcg gagaacgcgg taatcgaccg gatcgggacg gtcgcgggta
      541 atttggccgg tggcgacgtt ggggttcggg ttccggtagg tgcggtcgat attgaattga
      601 aggtagccga aaatgtcgtt ttcattggcc aacaaagccg aggggcatat cgccaagccc
      661 gcggcccaca acccggtcgc ccattttccc catttattca tccacctcgc gcgtgccgat
      721 gtcgctgctt tgttgcaagt ccggttaaag ctaacccaaa gaccgccgaa tgtcaaggcg
      781 tttcgtgttt tgcgcctccc cgtcgaattc ccgcggaact tgtgttatac tcacaccgcg
      841 ttaccgtgcg gcggtgcttc aaacggccgc cctcccgagg aggggcgatt tcccatggcg
      901 aacgaaggcc ccaccggcgg cccgcgggtt tccatcgatc cccggcgcct tcccgccttg
      961 gagacggagt ccgacgccgc cgaagcggcc ggaaattgcc ttcgggccct gccgccccgg
     1021 cgggagatcc tgaccctgat ccagggcctt tacggtccgg aaaacgccaa
//
LOCUS       DAOQLS010000838         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_132169_length_1070_cov_3.700493, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000838 DAOQLS010000000
VERSION     DAOQLS010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132169_length_1070_cov_3.700493"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK362_11905"
     CDS             complement(<1..721)
                     /locus_tag="PK362_11905"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNC75574.1"
                     /translation="MKKALWALGFLAAAGTAGARDFPKPRGAVSDFAGVLDADARARL
                     AALAADLESKTTAELAIVTVDSLAGDTVENTANAIFNAWGVGQKGKDNGVLILLAVGD
                     RKTRIEVGYGLEGTLNDGLCGEIIRAKMIPEFKAGRLAAGLEAGAQSVATIVAGGSVS
                     LPEESRGVEDRPWYPWVFGFVAVLFFGAVGYAFVLFAVLKTGVSFAGALVSGVAAMLL
                     RYLLRDQRHPIQSVLWGGGSGG"
     gene            complement(718..>1070)
                     /locus_tag="PK362_11910"
     CDS             complement(718..>1070)
                     /locus_tag="PK362_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC75575.1"
                     /translation="ESSEGFAWWGLGGSYRRLGRWAESRRAYGKALGLFRKSDDARGV
                     VMALLGLGAWAEETDRAIRAVNFRERALREARRHRLSYETALARWALDPRQVGGLGAF
                     GVSPATVKRWKDIP"
BASE COUNT          197 a          407 c          307 g          159 t
ORIGIN      
        1 ccccgccgct tcccccgccc cacaggaccg attggatcgg gtgtcgttga tcccggagga
       61 gataacgaag gagcatggcc gccacgcccg aaacgagcgc cccggcgaag gaaacgccgg
      121 tcttcagcac cgcaaaaagg acgaaggcgt aaccgacggc gccgaaaaac agaaccgcga
      181 cgaatccgaa gacccaggga taccagggcc ggtcctccac cccccgggat tcctcgggca
      241 aggaaaccga gcccccggcc acgatcgtcg ccacgctttg ggccccggct tcgagtcccg
      301 ccgcgagccg ccccgctttg aattccggga tcattttcgc acggatgatt tcgccgcaca
      361 aaccgtcgtt cagcgttcct tccaggccgt accccacctc gatccgagtc ttccggtccc
      421 ccacggccaa caatatcaaa acgccgttgt cttttccctt ctgcccgacg ccccaggcgt
      481 tgaaaatcgc gttggccgtg ttctccacgg tgtcgcccgc cagggagtcc acggtgacga
      541 tggccagttc ggccgtggtc ttgctctcca ggtccgcggc cagggccgcg aggcgggccc
      601 gggcgtccgc gtccaggacg ccggcgaaat cggagacggc cccccggggt ttggggaaat
      661 cccgggcccc ggccgtcccc gccgccgcca gaaaacccag ggcccaaagg gcctttttca
      721 tggaatgtct ttccagcgtt tcacggtcgc cggggatacc ccaaaggcgc ccaacccccc
      781 gacttggcgg ggatccaagg cccaccgggc caaggccgtc tcgtaggaca accgatggcg
      841 gcgcgcctcc cgaagggcac gctcccggaa attcaccgcc cggatcgccc ggtcggtttc
      901 ttcggcccag gctccgagcc ccaacaaagc catcacgacg ccccgggcgt cgtcggattt
      961 ccgaaaaagt cccagggcct tgccgtaggc ccggcgggat tccgcccaac gtcccaaccg
     1021 ccggtaactt ccccccaaac cccaccaagc gaacccctcg ctgctttcat
//
LOCUS       DAOQLS010000839         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_132383_length_1069_cov_4.185404, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000839 DAOQLS010000000
VERSION     DAOQLS010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132383_length_1069_cov_4.185404"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..830)
                     /locus_tag="PK362_11915"
     CDS             complement(165..830)
                     /locus_tag="PK362_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC75576.1"
                     /translation="MDIRRFLSTTSPFNVLPPAELDRLALTAHRSTVSKGDRVYNEGD
                     RAQFTFLVLGGRVQITRLARDGRPLTVELLKSGELFGCVGCSAAGQYPCEALAGQTTE
                     IIGFPMAIIVALIDRYPAFSRALYWDMSRRMRESQNMRALGAESVERRVAGVLLWLES
                     KFGPDLPFTRQAIAELASTTPESAIRTLIQFRRRGFIKTGWKKIVLQRTDALKELVEG
                     VAA"
     gene            complement(885..>1069)
                     /locus_tag="PK362_11920"
     CDS             complement(885..>1069)
                     /locus_tag="PK362_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75577.1"
                     /translation="GIRSFERNRTDIERFTGLTFPRAMLPNFPYKVTPKLEGPKPVSG
                     WQRIFAGRNRPLRRSR"
BASE COUNT          194 a          307 c          380 g          188 t
ORIGIN      
        1 ggggatccca aaccgagcaa cgcgccgacg tatccgtaac gccgagccca ggcggtgaac
       61 ggaacggcgc cgttgcgggc cgtccgggag ccggcggggg tcgtttcgtt gggcggcggg
      121 gacggctcgg tcatgaaact ctccagccgg cgcgccggct ttttttacgc ggcgacgccc
      181 tcgacgagct cttttaaggc gtcggttctt tggagaacga ttttcttcca gccggttttg
      241 atgaaaccgc gccggcgaaa ttggatgagg gttcgaatgg cggactcggg ggtcgtgctg
      301 gccagttccg cgatggcttg gcgggtgaag ggaaggtcgg ggccgaattt gctctccagc
      361 cacagcagca cccccgccac ccggcgctcc acggattccg cgcccagggc ccgcatgttt
      421 tgggattccc gcatacggcg gctcatgtcc caatacagag cgcgactgaa ggccgggtac
      481 cggtcgatga gcgccacaat gatcgccatg gggaacccga tgatttccgt ggtttgaccc
      541 gccagggctt cgcaggggta ttgcccggcg gccgaacaac cgacgcaccc gaagagttcg
      601 ccgcttttaa ggagttcgac ggtgagggga cggccgtccc gggcgaggcg cgtgatttgc
      661 acccggccgc ccaggaccaa aaacgtgaat tgggcgcggt cgccttcgtt gtacacccgg
      721 tcccctttgg aaaccgtgct ccggtgggcg gtcagcgcga ggcggtccag ctccgcgggg
      781 ggaaggacat tgaacggcga tgtggtcgaa agaaaacggc gaatgtccat ggaatcccca
      841 tgatagcatt tccgaccaaa ccgccggaaa accccggagc gggattaacg ggaacggcgg
      901 agggggcggt tgcgtccggc gaaaatccgc tgccagccgg acacgggttt cgggccttcc
      961 agtttggggg tgactttgta gggaaaattg ggaagcatgg cccggggaaa ggtgaggccg
     1021 gtgaaccgct cgatgtcggt gcggttgcgt tcgaagctgc ggatcccga
//
LOCUS       DAOQLS010000840         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_132412_length_1069_cov_3.425049, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000840 DAOQLS010000000
VERSION     DAOQLS010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132412_length_1069_cov_3.425049"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PK362_11925"
     CDS             <1..886
                     /locus_tag="PK362_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC75578.1"
                     /translation="GWSVSTKSLGFWDYRGEPGGVEFTALNAEGEKKFRTAIPLDYPR
                     TDSPDAYAGPGPLGQPLALADGGWLLSGETSGADGAGGRVFRLDGQGRLIWKAEGRAL
                     ALSRTTVVVDRGFDGVAGLNRENGEERWTKTPAGAEVPGGGADMWGFILSRGSEKGFH
                     VYDAETGERRFILPCMGKTDLELKGQLHFSHGAAILYAEPLEIIDIVDGQGKRRARWR
                     SAGRFVGFDGRGALFVTREGDWALYDADKPEAVPAVLKNRDIPDGELLKPFPDRLVVG
                     DGGVAAFNAAWGRGWIFE"
BASE COUNT          192 a          264 c          410 g          203 t
ORIGIN      
        1 ggggtggtcg gtctcgacga aaagcctggg gttctgggac taccggggag aaccgggcgg
       61 agtggagttc accgccctga acgcagaggg agaaaagaaa tttcgaacgg cgatccccct
      121 ggactacccc cggaccgatt caccggacgc ctacgccgga ccggggccgt tgggtcaacc
      181 cctggccctg gccgacgggg gatggctttt aagcggggag acatccggcg cggacggcgc
      241 gggcgggcgg gtgtttcgcc tcgacgggca agggcggttg atttggaagg cggaaggccg
      301 ggcgttggcc ctttcccgaa caacggtggt cgtggatcgg ggttttgacg gtgtggcggg
      361 gctgaaccgg gagaatggcg aggagcggtg gacaaaaacg ccggccggtg ccgaggtccc
      421 cggcgggggg gccgacatgt ggggatttat tctctcccgg ggaagcgaaa aagggttcca
      481 cgtatacgac gcggagacgg gggaacgtcg tttcatcctg ccttgcatgg gaaaaacgga
      541 tttggaactg aaagggcaac tgcactttag tcacggagcg gcgattctat acgcggagcc
      601 cctggaaatc atcgacatcg tggacggcca aggaaaaagg cgcgcccggt ggcggagcgc
      661 ggggcgcttc gtggggtttg acggtcgggg ggccctcttc gtgacccggg agggggattg
      721 ggccctatac gacgccgaca aacccgaggc ggtcccggcg gtcctaaaaa acagggatat
      781 tcccgacggg gaattgctga agccttttcc cgaccgactg gtcgtcggcg acgggggcgt
      841 ggcggcgttt aacgcggcct gggggcgggg gtggattttt gagtaggagg gcgtgtggct
      901 tactattttt ttaaatttct gatcaccagt ttgttggtgg tgtttatttc cgaaatcgcc
      961 aagcggagct cttttttggg ggcggtgttg gcgtccttgc cgctcacgtc ggtcctggcg
     1021 atgatttgga tttaccggga gacccgggag gcggggaagg tggcggacc
//
LOCUS       DAOQLS010000841         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_132620_length_1068_cov_4.394867, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000841 DAOQLS010000000
VERSION     DAOQLS010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132620_length_1068_cov_4.394867"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..513)
                     /locus_tag="PK362_11930"
     CDS             complement(94..513)
                     /locus_tag="PK362_11930"
                     /inference="COORDINATES: protein motif:HMM:NF022795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3TM-type holin"
                     /protein_id="HNC75579.1"
                     /translation="MALDPISNIAGAVKGIIDKFVPDANEKLKMQLELATKEIVQEHE
                     RLLAQANTNLEEAKNPNVFVSGWRPAAGWVCVLGLAYTFLLHPYMVWISTLASFAAPP
                     PIESGLLQYLLGAMLGIGGLRTWDKVKGVETKGVSIK"
     gene            complement(515..940)
                     /locus_tag="PK362_11935"
     CDS             complement(515..940)
                     /locus_tag="PK362_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNC75580.1"
                     /translation="MEVKMGNDPKIPPTSRNISDAANELKGKWALIQQRFHDLTGNDL
                     ILTCTHRTVAEQQRLYSQGRTTKGNIVTWVDGVTKKSNHNYYPSKAIDVAVVIGGKVS
                     WDPKDYQPLGIICKDLNLTWGGNWKPPKTDLPHIELQGA"
BASE COUNT          197 a          302 c          301 g          268 t
ORIGIN      
        1 gacggtattc gtaggtcttg tgcgccgacg tcgtgaggtt cacgacgggc caccccgtct
       61 tggtgatgag ttctttttct ttgcgtgatc gtttcatttg atcgacaccc ctttcgtctc
      121 cacgcccttt accttgtccc atgtccgaag tccgccgatc ccgagcatgg ccccgaggag
      181 atactgcaat agaccggact cgattggagg cggagccgcg aacgacgcca gagtggaaat
      241 ccagaccatg taggggtgaa gaaggaaggt gtaggccagg ccaaggacgc acacccatcc
      301 agcggcggga cgccaacccg acacaaagac attcgggttt ttggcttcct ccaggttcgt
      361 gtttgcctgg gccaaaagcc tctcatgctc ctgaacaatc tccttggtcg caagctcaag
      421 ctgcatcttc agcttttcgt tggcgtcggg gacgaacttg tcgatgatgc ccttgaccgc
      481 gccggcaatg ttggagattg ggtctaaggc catgtcatgc cccctggagt tcgatgtggg
      541 gcaggtctgt ttttggtggt ttccagttcc cgccccatgt gaggttcaaa tccttgcaga
      601 tgataccgag cggctggtag tccttcggat cccacgacac cttcccgccg atgaccacgg
      661 cgacgtcgat cgccttggac gggtagtagt tgtggttcga cttcttcgta accccatcca
      721 cccacgtcac gatgttgcct ttggtcgtcc gcccctggga gtagagccgt tgctgttccg
      781 ccaccgtcct gtgagtgcag gtcagaatga ggtcgtttcc ggtcaagtca tggaaccgct
      841 gttgaatcag cgcccacttt cctttcaatt cgttggcggc gtcggagatg tttctgcttg
      901 tcggcggaat cttaggatcg tttcccattt ttacctccaa agtattcctc ataaaatcgg
      961 tcggattggg ccagtttttg gtggaggatt atccggtggc aaatcccgcc acggacgtaa
     1021 tggaagtcga cgctcggttc acaccggcaa ttcccgggac cgccgaag
//
LOCUS       DAOQLS010000842         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_132751_length_1068_cov_2.105627, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000842 DAOQLS010000000
VERSION     DAOQLS010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132751_length_1068_cov_2.105627"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1068
                     /locus_tag="PK362_11940"
     CDS             23..>1068
                     /locus_tag="PK362_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75581.1"
                     /translation="MVGPITLPGLQYDTTYYIGVAALDDEYARNQSLLLAGLRCTLGP
                     TPVVNSAIADEVLLTVTVDVDLFNTRTGHSIKWQARIAEDAGFSVNVTTNGVKTTINP
                     TDTDVFTGRSAGKTYYTQARTINQAGAPSPWSPTKVAVFRNGIPVMTAIPSTDVFPSS
                     ITARWDNSSVPNGYKLQAQADATESDLPNGNPTGVFDFTSGGLRTTPAFSSYSFNSGM
                     TPNTTYFIQVESFYPAGGIVGTAPYVSAMTGVPFPTLTVANVYITSVTYTITESGVPN
                     THRYFVELSTTSNFSSGVATAAVSNFGAADEIRNLDLVNLIGNTTYYSRLTAFNHVNW
                     QSPTTNPGPSFITRP"
BASE COUNT          215 a          362 c          275 g          216 t
ORIGIN      
        1 tgggggattt ccggccgggc aattggtcgg cccgatcact ctgcccggcc ttcaatacga
       61 cacgacctac tacatcggcg tcgcggccct ggacgatgaa tacgcccgca atcagtcgct
      121 tttattggcc gggcttcgtt gcactctggg gcccacgccg gtggtcaatt ccgccatcgc
      181 cgacgaagtg ctgctgaccg tcaccgtcga cgtcgattta tttaacacgc gaacgggtca
      241 ctcgatcaag tggcaggcgc gcattgccga agacgcgggt ttttccgtca acgtgacgac
      301 caacggtgtt aaaacgacga tcaatcccac cgacacggac gtgttcaccg gtcgctccgc
      361 cggtaaaacc tactacaccc aagcccggac gattaaccag gccggggcgc cgtcgccctg
      421 gagccccacg aaagtggccg ttttccgaaa cggcattccc gtgatgacgg ccatcccctc
      481 caccgatgtg tttccgtcct ccattacggc ccggtgggac aacagcagcg tcccgaacgg
      541 gtataaacta caggcgcagg ccgatgccac cgagtcggat ctgccgaacg ggaaccccac
      601 gggggtcttc gattttacca gcggcggact tcgaaccacc cccgcgttct catcctattc
      661 ctttaactcc gggatgacgc ccaacacgac gtattttatt caagtggagt ctttctatcc
      721 cgccggggga atcgtcggaa ccgcccccta cgtgtcggcc atgacgggcg tcccattccc
      781 gactttgacc gtcgccaacg tgtatatcac cagcgtcacc tacaccatca ccgagagcgg
      841 cgtcccgaac acgcaccggt attttgtcga actttcgacc acctccaact ttagcagcgg
      901 ggtggcgacc gccgcggtct ccaacttcgg ggccgccgat gaaattcgca atttggattt
      961 ggttaactta atcggcaaca ccacctacta ttcccggttg acggccttca atcacgtaaa
     1021 ttggcagtcg ccgaccacca accccggtcc gtccttcatc acgcggcc
//
LOCUS       DAOQLS010000843         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_132875_length_1067_cov_3.502964, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000843 DAOQLS010000000
VERSION     DAOQLS010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132875_length_1067_cov_3.502964"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /gene="rimI"
                     /locus_tag="PK362_11945"
     CDS             <1..433
                     /gene="rimI"
                     /locus_tag="PK362_11945"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNC75582.1"
                     /translation="PWAEAPLSDLVQLETQSSPSPWSRRQFEEELAAPRSRGLAYLSA
                     DGRARAFGVYWWIPPEAQLANLAVEPALRGRGLGRALLERLMARAVAEGGRLATLEVR
                     LSNAPALALYRRLGFRETGRRPRFYEGTETAVLMEKTLDPR"
     gene            420..>1067
                     /locus_tag="PK362_11950"
     CDS             420..>1067
                     /locus_tag="PK362_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC75583.1"
                     /translation="MTPDDRSSRRILVGVAALLGAGAVGASPWEGYRLFLRGLLEERA
                     GRPAAAAEMYENVLKRDPEAPAVRAALVEACLAAGRWDAALQTARSLADGFPESVEFQ
                     ILLGRAYLAAGQSDKAQGAFDRALKLDPANVDALGWATSQTRLTNPAVAAGALEKFLK
                     ESGDGEVADAVRDRLAKLLEHEGNAAGAERHWKKILESDPGNVDALLGLAGLYDVR"
BASE COUNT          160 a          364 c          369 g          174 t
ORIGIN      
        1 tccctgggcc gaagcgccgc tgtccgatct cgtccaactg gaaacccaaa gttccccgtc
       61 cccctggtcc cggcggcaat ttgaggaaga actcgccgcg ccgcggtccc ggggactcgc
      121 ctacctttcg gccgacggtc gggcccgggc cttcggcgtc tattggtgga ttccgccgga
      181 agcccagctg gccaacctgg cggtcgagcc cgccctccgc ggacgggggc tgggccgcgc
      241 ccttctggaa cgtttaatgg cccgggccgt cgccgagggc ggccgtctgg cgaccctcga
      301 agtccggctc tccaacgccc ccgccctcgc cctttaccga cgtttgggtt ttcgcgaaac
      361 cggccgccga ccgcgcttct acgagggaac cgaaaccgcc gtcctgatgg agaaaaccct
      421 tgacccccga tgaccgatcc tcccgccgga ttctcgtcgg ggtcgcggcg ctcctgggcg
      481 ccggggcggt cggcgcgtcc ccctgggaag gataccgcct cttccttcgg ggcctgttgg
      541 aggaacgggc gggccggccc gccgcggccg cggaaatgta tgaaaacgtt ttaaagcggg
      601 atcccgaagc gccggccgtt cgagcggcgc tcgtcgaggc gtgcctcgcg gccgggcgtt
      661 gggacgccgc gcttcaaacc gcccggagcc tggccgacgg ttttcccgag agcgttgaat
      721 tccaaattct gctgggccgg gcctatttgg ccgccggcca atcggacaaa gcccaggggg
      781 ctttcgaccg ggcgctgaaa ctggatcccg ccaacgtgga cgcgctcgga tgggccacca
      841 gccaaacccg gctcaccaat ccggcggtgg ccgcgggggc gttggaaaaa ttcctcaaag
      901 agtcggggga cggggaggtg gccgacgccg tccgggaccg gctggccaag ttgctggagc
      961 acgaagggaa cgccgccggc gccgagcgtc attggaagaa gattttggag tcggatcccg
     1021 ggaacgtcga cgccttgctg ggtttggcgg gtctttacga cgtccgg
//
LOCUS       DAOQLS010000844         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_132901_length_1067_cov_2.966403, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000844 DAOQLS010000000
VERSION     DAOQLS010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132901_length_1067_cov_2.966403"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(679..>1067)
                     /locus_tag="PK362_11955"
     CDS             complement(679..>1067)
                     /locus_tag="PK362_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75584.1"
                     /translation="IEKSVEVRAGIKAIRYSQKSVGLDVALRLKKEVSSVDKRRIEKP
                     FSTSGFGSEDKPSDAALPPAPKSNEPNRMSRLGSSLKFDSQQMAGFSTTFEITLPLNF
                     PNIAHGYWKNYKLTGEFSPARSKLSV"
BASE COUNT          206 a          296 c          349 g          216 t
ORIGIN      
        1 gacaaccgac aggccgaacg gcctggcggt ggcggggcgt ccgaacgtca gcgggcaaca
       61 tcttttcccc gactgtggag ctgggccgcc gcgtggggtg tcggggcgga ccggcggacg
      121 tttcgagggc ggtggggttc cgaatcaatc ggagcaaacc gccggggttc ttttcccaac
      181 caaccaagcg agccgtgcgg cggcgcgggg gtttcgtgcc gacgcgcggc gagcgacctt
      241 ccagcgtttc cgccggagcg acccgccggg ttttcttttc ccggaaccga accaagcgca
      301 gtgggttttt ccggtgctgt tggttttgat gttcgccggg ttttggtttt tcggatcacc
      361 aggcgaatcg atcccgggac gcggacgcta tgcgctcgtg atgtcattgc tgatggtggg
      421 gtggcgatac gtcacggtgg gcggcccggg tatagcggtc acaaattcaa agaaagggcg
      481 gaagatgaaa aaaacgcgaa cccatgcggt gggcctggcg gccgacggat gtggcggctg
      541 aatcgacaga caaggcccca caatccgggt cctgcccgcg aggtagggcg cggcttcctg
      601 cgccgcgcca cagtcaagcc ttgggggggc ccccaaggcc agcggggccg caatcgcggt
      661 cgcaatcaca ccgagggttc aaaccgacaa tttagatcgg gctgggctga attccccggt
      721 caatttgtag tttttccaat acccgtgagc gatgttcggg aaattgagcg gaagcgtaat
      781 ttcgaaggtg gtggaaaacc ccgccatttg ctgagaatca aacttcaaag acgagcccaa
      841 tcgagacatt cggttgggtt cgttgctttt aggggcgggc ggaagggcgg cgtccgaggg
      901 cttgtcctcc gaaccgaaac cggaagtgga aaacggcttt tctattctcc ttttatcgac
      961 ggaagaaacc tcctttttca accgcagggc cacgtccagt ccgacactct tttgcgaata
     1021 gcggatcgct ttgatcccgg ctcgaacctc cacagatttt tcaatcc
//
LOCUS       DAOQLS010000845         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_133092_length_1066_cov_3.867458, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000845 DAOQLS010000000
VERSION     DAOQLS010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133092_length_1066_cov_3.867458"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PK362_11960"
     CDS             complement(<1..1045)
                     /locus_tag="PK362_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75585.1"
                     /translation="MPNVRRLATALARERSMDFPRVESERREFLARLSKALNERENAE
                     LLHRVVEHRAGRLGYGALFDALDGFARRHGLTLSDYPALTAYRRYAEATESIERGPLL
                     TELADLERRVEDALAVGPRARRLLVLERRWRLLTQLVDLSLSPDGWSAYLVERDALRR
                     WPADLAALDVDLPRFPSPESLRPFESFCERASRRNDALVANTLHRMRRDGARVAVLVA
                     GGFHTPGLEKALVRREVAVGVYTPRVDVLPAGNPGLAALVEDPRPLEKIFTGEPLSLV
                     SERPLGPSINLDGTRQRAVEGLLAAQIAAPLAVGDVTVAGANERLAALAGETLATIDR
                     ARVTAPRRAGAPYE"
BASE COUNT          164 a          381 c          387 g          134 t
ORIGIN      
        1 attcgtaggg cgccccggcg cgacgggggg ccgtcacccg agcccgatcg atggtcgcga
       61 gggtctcccc cgccaaggcc gccaggcgct cgttggcccc ggccaccgtc acgtcgccga
      121 ccgccagggg ggcggcgatc tgggcggcca ggagcccctc caccgcccgc tggcgggttc
      181 cgtcgaggtt gatggacggc cccaggggcc gctcgctcac gagggagagc ggctccccgg
      241 tgaatatttt ttccagggga cgggggtcct ccaccagggc cgccaatccc gggttgcccg
      301 cgggcagaac atcgacccgg ggcgtgtaga ccccgaccgc gacctcccgg cggaccaggg
      361 ccttctccag tcccggggtg tgaaacccgc cggccacgag gacggcgacc cgcgccccgt
      421 cccggcgcat ccggtgcaga gtgttggcga cgagcgcgtc gttccgccgg gaagcgcgtt
      481 cgcaaaagga ctcaaagggc cggagggatt cgggggacgg gaagcgcggc aagtcgacgt
      541 cgagcgccgc caggtccgcc ggccaacggc gcagggcgtc ccgttcgacc aaataggcgg
      601 accagccgtc cggcgagagg gacaaatcca ccagttgcgt caagagccgc catcgccgct
      661 ccaggaccag aagccgtcgg gcccgggggc cgacggccag ggcgtcttcc acgcgccgct
      721 cgaggtccgc caattccgtg agaaggggcc ctcgctcgat gctttcggtc gcctcggcgt
      781 aacgccgata agcggtcagg gcgggataat ccgagagcgt caggccgtgg cggcgcgcga
      841 acccgtccag ggcgtcgaac agcgccccgt agccgagacg gcccgcgcgg tgttccacca
      901 cccggtggag tagctcggcg ttctcccgct cgttcagggc tttggacaac cgggccagga
      961 attcccgacg ttcgctttcg acgcgcggaa aatccatgga ccgctcccgg gccaaggccg
     1021 tggccaaccg gcgaacgttc ggcaccgggg cggcggccgc tccggg
//
LOCUS       DAOQLS010000846         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_133340_length_1065_cov_4.543564, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000846 DAOQLS010000000
VERSION     DAOQLS010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133340_length_1065_cov_4.543564"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..826
                     /gene="sfsA"
                     /locus_tag="PK362_11965"
     CDS             86..826
                     /gene="sfsA"
                     /locus_tag="PK362_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HNC75586.1"
                     /translation="MEAQVVFSPPLTTGVLLKRYKRFLADVKLDSGKVITALCPNTGS
                     MKSCCEIGRPVALSYHPDPGRKHRYTWEMIRMDAGWVGVNTGVPNGLAALAIKYGQIP
                     EFISYTEVKREVRLGVNSRIDLLLEGPPGRCWVEIKNVSMLEGDAVTFPDAVTERGKK
                     HLEELCGALAKGDRAAMLFVVQRPEGKVFRPADAIDPEYGAALRRAAELGVDVLVYRA
                     KVGRERVAWGEAVPLDLSPQTPKETSRV"
     gene            819..>1065
                     /locus_tag="PK362_11970"
     CDS             819..>1065
                     /locus_tag="PK362_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNC75587.1"
                     /translation="MFEKMKQLMDMQKMAKEAERRLEEIAIERAALGGKLRAKVTGNQ
                     RVAAIEIDAALAAPGQKEVLEKALVELLNDALGDAKKE"
BASE COUNT          211 a          336 c          351 g          167 t
ORIGIN      
        1 gggccggggc gccccgggcc cgcaaggcgg ggcgccaact ggccccccag gatgtggacg
       61 acatcaacga ggagcttcgg cgactgtgga agctcaagta gttttttctc cgcctctcac
      121 gacgggcgtt ttgctcaagc gttacaagcg ctttttggcc gacgtgaagt tggattcggg
      181 caaggtgatc acggcccttt gccccaacac cgggagcatg aagagctgct gcgagatcgg
      241 ccgccccgtg gccttgagct accaccccga tcccggccgc aaacaccgct acacctggga
      301 aatgatccgc atggacgccg gttgggtggg ggtcaacacg ggcgttccca acgggttggc
      361 cgccctggcc atcaaatacg gccaaatccc ggagttcatc agctacaccg aggtaaaacg
      421 cgaggtgcgc ctcggcgtca acagccgcat cgacctgttg ttggaaggcc cccccggtcg
      481 ctgttgggtc gagatcaaaa acgtgagcat gctcgaaggc gacgccgtca cctttcccga
      541 cgccgtgacc gagcggggca agaaacattt ggaggaactc tgcggcgccc tggccaaggg
      601 cgaccgggcc gccatgttgt tcgtcgtcca gcgccccgaa ggcaaggtct tccgtcccgc
      661 cgacgccatc gacccggaat acggcgccgc cctccgccgg gccgcggagt tgggcgtcga
      721 cgttttggtt taccgcgcca aggtgggccg cgaacgcgtg gcctggggcg aggccgtgcc
      781 cctggattta tcccctcaaa cccccaagga gacgtcccgt gtttgaaaaa atgaagcagt
      841 tgatggacat gcaaaaaatg gccaaggagg ccgaacgccg cctggaggag atcgccatcg
      901 aacgcgccgc cctcgggggg aaactccggg cgaaggtgac gggcaaccag cgcgtcgccg
      961 ccatcgagat cgacgccgcc ctggcggcgc ccgggcagaa agaggttttg gagaaagccc
     1021 tggtggaact tttgaacgac gccctgggcg acgcgaagaa agagg
//
LOCUS       DAOQLS010000847         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_133363_length_1065_cov_3.503960, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000847 DAOQLS010000000
VERSION     DAOQLS010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133363_length_1065_cov_3.503960"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PK362_11975"
     CDS             complement(<1..874)
                     /locus_tag="PK362_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNC75588.1"
                     /translation="MTRLHRYVLSMFLRPLLFSYGALTILVLMAETMERLDKFIAGKA
                     TVGVVAQYLLAVWPLRSQELLPVSALLAALFALGQLSRRMEITAALSGGIHPWQLTAP
                     LLVTGVVLSVFSWGVGEAVNPWASRQAKALWNADVRHLTSFRPTKFEDVTVAGKGVFY
                     SIGLLDTDHQTLRDVVVDVTHGDRPRIQWQARSGVWTDRGWELIDGFERRFSPDGRAL
                     ERQTAFATRWIDRREKATDLVPQEPDTEEMNRHDLRQHIDRLKILGIPTQKLQVELHM
                     KTAFPWANFIVLLIG"
     gene            complement(871..>1065)
                     /locus_tag="PK362_11980"
     CDS             complement(871..>1065)
                     /locus_tag="PK362_11980"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNC75589.1"
                     /translation="TLERGGRGVGFGAAMGVLFLYYLLLVMGLNVAEKGKWPAAPALW
                     VGNVAALAVGAELFRRRLAR"
BASE COUNT          209 a          350 c          333 g          173 t
ORIGIN      
        1 ccccgatcaa cagaacgatg aaattggccc aggggaaagc cgttttcatg tgcagttcca
       61 cctggagttt ctgggtcgga atgcccagaa tcttcaatcg atcgatgtgt tgtcggagat
      121 cgtgccggtt catttcctcg gtgtccggtt cctggggcac gaggtcggtg gccttttccc
      181 gacggtcgat ccaacgcgtg gcgaaggcgg tctgacgttc cagggcccgt ccatcggggg
      241 aaaaccgccg ttcgaatccg tcgatcagtt cccatccccg gtcggtccag accccgctcc
      301 gggcctgcca ttggatccgg ggccggtcgc cgtgggtgac gtccaccacc acgtccctca
      361 gggtttggtg gtcggtgtcc agcaaaccga tggaatagaa aacgcccttt cccgccacgg
      421 tgacgtcctc gaatttcgtc gggcggaagg acgtcaaatg gcgcacgtcg gcgttccaca
      481 gggctttggc ctgccggctg gcccagggat tcacggcctc gcccacgccc caggaaaaaa
      541 cgctcaacac gaccccggtc accaagaggg gggcggtgag ttgccagggg tgaatgccgc
      601 cggaaagcgc ggccgtgatt tccatccggc gggagagctg ccccagggcg aagagggccg
      661 ccaatagggc cgagaccgga agcagttcct ggctgcgcag gggccaaacg gccagcaaat
      721 attgggccac cacgcccacc gtggcttttc cggcgatgaa tttgtccagg cgctccatcg
      781 tctccgccat gaggaccaga atcgtcagcg ccccgtagct gaaaaggagg gggcgcagaa
      841 acatggacaa aacgtaacgg tggaggcggg tcaccgggcc agccgccgtc ggaaaagctc
      901 cgcccccacg gccaaagccg cgacgttgcc gacccacaaa gccggcgcgg cgggccattt
      961 tcccttctcg gcgacgttca accccatcac caacagcagg taatagagga aaagcacgcc
     1021 catggcggcc ccgaacccca ccccccggcc gccccgctcc agggt
//
LOCUS       DAOQLS010000848         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_134702_length_1060_cov_3.132338, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000848 DAOQLS010000000
VERSION     DAOQLS010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134702_length_1060_cov_3.132338"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1060)
                     /locus_tag="PK362_11985"
     CDS             complement(<1..>1060)
                     /locus_tag="PK362_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75590.1"
                     /translation="EWGVGGSFLGLGIFAALHGFLDRTALRDRAGPFAALRWAVEQFR
                     RHYGLLLFAYLPLAFAGPIPGALAWAVLVHVAYDLERLFPETFQHLTRFLDQGPLLAG
                     AWVVPPGSAPVPVTLRSLFVRGFSPEELFAFDQRLPERGPEVFARYHTRSVVVAASRV
                     RGIQSRARAFYDEAPGLGWPPSKIGQIRKAVEAVAALYNPQRVHWFEAEVRGPGEHVL
                     AWPDAFAADVVEFLVRRERALGIPGLVQNYLLTAMLEFKTGLSEFERLEIVGILVGQA
                     PAAAREALTRALADFTAEKVPAAPTESPAAIPATPARSERRSLAQWVYAWTGAVQATA
                     FPPLDARGRPGVQEFDFAE"
BASE COUNT          210 a          358 c          359 g          133 t
ORIGIN      
        1 cctcggcaaa atcgaattcc tgaacgccgg gccgcccccg ggcgtcgagg ggcggaaagg
       61 ccgtggcctg gacggctccc gtccaggcgt aaacccattg ggcgagggac cggcgctccg
      121 agcgggcggg ggtcgccgga atggcggcgg gcgactccgt cggcgccgcc ggaaccttct
      181 ccgccgtgaa atccgcgagg gcccgcgtca aggcttcgcg cgccgccgcc ggggcctgtc
      241 ccaccaatat cccgacgatc tccaggcgtt cgaattccga aaggccggtt ttgaattcca
      301 acatcgcggt caataaataa ttctgcacca acccgggaat ccccagggcc cgttcccgac
      361 ggaccaggaa ttccacgacg tcggcggcga aggcgtcggg ccaggcgaga acgtgttccc
      421 cgggtccccg aacttccgct tcgaaccaat ggacccgttg gggattgtaa agggccgcca
      481 cggcctcgac ggccttccgg atttgtccga ttttggacgg gggccagccc aaccccggcg
      541 cctcgtcgta aaaagcccgg gcgcgtgatt ggatcccgcg cacccgcgac gccgccacga
      601 ccaccgaccg ggtgtgatac cgggcgaaca cctcggggcc gcgttccggc aaccgttggt
      661 caaaggcgaa aagttcctcg ggcgaaaaac cccggacaaa cagggagcga agcgtgaccg
      721 ggaccggggc gcttccgggc gggacgaccc aggcgcccgc caacagcggt ccctggtcca
      781 gaaaacgggt gaggtgttga aaggtctcgg ggaaaagccg ttccaaatcg tacgccacgt
      841 gaaccaacac cgcccaggcg agggccccgg ggatcggacc ggcgaaggcc agggggaggt
      901 aggcgaagag caacaggccg tagtgacgcc ggaattgttc cacggcccat cgaagggccg
      961 cgaagggccc ggcgcggtcg cgaagggcgg tgcggtccag aaaaccgtgg agcgccgcaa
     1021 agatccccaa cccgaggaac gacccgccga ccccccattc
//
LOCUS       DAOQLS010000849         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_135419_length_1057_cov_3.504990, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000849 DAOQLS010000000
VERSION     DAOQLS010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135419_length_1057_cov_3.504990"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1057
                     /locus_tag="PK362_11990"
     CDS             <1..>1057
                     /locus_tag="PK362_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HNC75591.1"
                     /translation="DSLARNSLLLLSGKQTLRVAGRPVTATQWFLDTLFRPEVADGYA
                     VFEINDPDVLGLLGIQQGSQRRYAFKDLAPHLEAIETQAHHAEGIRSEDRSRFQTAVG
                     ALQGKLILYQKIQNTLQISSVADWQGELAAFEGALAERNAPATRAKKKSDPHGGPAML
                     ASFRDRYAFLSQAAEFLPLPVPGPDPVHWVSVGATLLVRFEAPALHPGVPAYARMGDA
                     YRAGDTAAFGEAVDDYRAWLMENVPSPTRRVGSEVLFNRFAPFYKALLLYGLVALLIF
                     ISFLAWPEPLRKTASHVLWIAFLVHTFGLVSRIVLQGRPPVTNLYSSAVFVGWVAVLL
                     GIVLERLYKNGLGALGAA"
BASE COUNT          150 a          351 c          329 g          227 t
ORIGIN      
        1 ggattccctg gcgcggaatt cccttctgct cctttcggga aagcagaccc tgcgcgtggc
       61 cggtcggccg gtgacggcca cccaatggtt tctggacacc ctgtttcgcc ccgaggtggc
      121 cgacgggtac gctgttttcg agatcaacga tcccgacgtt ctggggcttt tgggcattca
      181 gcaggggtcc cagcgtcgtt acgcctttaa ggacctcgcc ccccacctgg aggccataga
      241 aacccaggcc catcacgccg agggcatccg ttccgaggac cggtcccggt ttcaaacggc
      301 cgttggggcc ctgcagggca agttgatcct ttatcaaaag attcaaaaca cccttcagat
      361 ttcctcggtc gccgactggc aaggcgaatt ggccgccttt gaaggcgccc tggcggaaag
      421 gaacgctccc gcgacccggg ccaaaaagaa atccgacccc cacggcggac cggcgatgct
      481 ggcgtccttc cgtgaccgct acgccttttt gtcccaggcc gccgagttct tgccgctgcc
      541 ggttcccggt ccggacccgg ttcattgggt cagcgtcgga gccaccttgt tggttcggtt
      601 cgaagccccg gcccttcacc cgggggtgcc ggcctacgcc cgcatgggcg acgcctatcg
      661 ggccggcgac accgccgcct tcggcgaggc cgtggacgat taccgggcct ggctgatgga
      721 aaacgttccg tccccgaccc gccgggtggg ttcggaggtt ctgttcaacc gcttcgcccc
      781 gttctacaag gcgctcttgt tgtacggcct ggtggcgctc ttgattttca tctctttcct
      841 ggcctggccg gaacccctgc ggaagacggc gtcccacgtt ctttggatcg ccttcttggt
      901 ccataccttc gggctcgttt cccgcattgt tcttcagggg cgtccgccgg tgacgaacct
      961 ctattcgtcg gcggtgttcg tggggtgggt cgccgttctc ctgggcatcg tgctggagcg
     1021 gctctacaaa aacgggctgg gggccctggg cgcggcg
//
LOCUS       DAOQLS010000850         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_136040_length_1055_cov_2.529000, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000850 DAOQLS010000000
VERSION     DAOQLS010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136040_length_1055_cov_2.529000"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1055
                     /locus_tag="PK362_11995"
     CDS             <1..>1055
                     /locus_tag="PK362_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75592.1"
                     /translation="YLGAWWAALLHWFMDVSVRFVSSGIFSFLLPGLVVSLSRHHDHP
                     ERQDAPASTDRALRWGIILFWALVFVHGVFAIPSIRARPLGHIVGLVVFVGLMAGLAE
                     LLEWRLVDASAAATERPRGPGGAPAAWQWVCGGAAVLLWGACYGVFRNYFIADINHNV
                     GIFFSKQGMWTRSSEFDARVSAPDYPYEMRKEYEEQGGALEHYETVCRLNPNFPMARY
                     FIGNVYNDWGAGIMDRAKELKGRGDAGAAEVLHKRAAEYLDKSLAAYDAVKAFAPNYV
                     QTHHQVGLVYLKKAEMESLFGDKSKSNDYWDKALQNFGLYKNLDPVFPPNHYRESYVH
                     FMRGDYEKAEKAYLNALT"
BASE COUNT          202 a          304 c          315 g          234 t
ORIGIN      
        1 tacctggggg cctggtgggc ggccctcctc cattggttta tggacgtttc cgtccgcttt
       61 gtgtcctccg gaatcttttc ctttctcctt ccgggactcg tggtttccct gtctcgccat
      121 cacgaccacc cggaacgcca ggacgcgccc gcctccacgg atcgggcgct ccgctgggga
      181 ataattcttt tttgggcgtt ggtgttcgtc cacggcgttt tcgcgattcc gagcatacgc
      241 gctcgacctc tcgggcacat cgtcggtctc gtggtgttcg tcggtctcat ggccgggttg
      301 gccgaactat tggaatggcg attggtggac gcttccgcgg cggccaccga acgtccccgc
      361 gggccggggg gcgctcccgc cgcctggcag tgggtctgcg ggggcgcggc ggtacttctc
      421 tggggggcct gctacggggt gttccgaaat tactttattg ccgatatcaa ccacaacgtc
      481 ggcatttttt tctcgaaaca agggatgtgg acgcggtcct cggagtttga cgcccgggtt
      541 tcggcgccgg actatcctta cgagatgagg aaagaatacg aagagcaagg cggcgctttg
      601 gagcattacg agaccgtctg tcgactgaat ccgaattttc ccatggcgcg ttatttcatc
      661 ggaaacgttt acaacgattg gggcgccgga atcatggatc gggccaaaga actgaagggc
      721 cgcggcgacg ccggggcggc cgaggtcctg cacaaacgcg cggccgaata cctggacaaa
      781 tccctggcgg cctacgacgc cgtaaaggcc tttgcgccca attacgtgca aacccaccac
      841 caggtgggcc tggtctattt gaaaaaggcg gaaatggaaa gcctcttcgg tgacaaatcg
      901 aaatccaacg attattggga caaggccctg caaaatttcg ggctgtacaa aaatcttgac
      961 ccggtattcc cgccgaacca ctaccgggaa tcctacgtgc atttcatgcg cggcgattac
     1021 gaaaaagccg aaaaggccta tttgaacgcc ttgac
//
LOCUS       DAOQLS010000851         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_136093_length_1055_cov_2.076000, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000851 DAOQLS010000000
VERSION     DAOQLS010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136093_length_1055_cov_2.076000"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK362_12000"
     CDS             <1..429
                     /locus_tag="PK362_12000"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNC75593.1"
                     /translation="EWGPSLDGRGHPLTRWFRRSPVAPETPGARAEQLARRYLEKKGF
                     RFLAANHRTRFGEVDLVMEEGTTVVFVEVKARSRSDFGRPEEFVTAAKQARVAKAALD
                     FVKARGLSDRPLRFDVVGLLGEEIAHLPNAFVPEAGRYLF"
     gene            complement(433..>1055)
                     /locus_tag="PK362_12005"
     CDS             complement(433..>1055)
                     /locus_tag="PK362_12005"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNC75594.1"
                     /translation="CGQLAAMDFETLYTRFGAWGHWLKRMGRGQDDAPVSRVDAVDTV
                     KSVGHATTFPANTRDPDVLKAYVMFLSEKVAARARRYRLAGREVVLTVRDADFKTRTH
                     HRALGEAIFTDDAIFAAAVGLLTEKERLEKPVRLVGVCLARLEPDGGQPSLFKESERP
                     RKLAETLDRLNARYGKGTVGRARAALAKKHGVLSPPVPPVLRAADD"
BASE COUNT          181 a          343 c          346 g          185 t
ORIGIN      
        1 gaatgggggc cgagcttgga cgggcgaggc catcccttga cgcgctggtt ccgccgctcg
       61 cccgtcgcgc cggaaacgcc cggggcccgg gcggaacagc tcgcccgacg gtatttggaa
      121 aagaagggct ttcgattttt ggcggccaac caccgcacgc gtttcgggga agtggacttg
      181 gtgatggagg agggaacgac ggtggtgttc gtcgaagtga aagcccggtc tcgatcggat
      241 ttcggccgcc cggaggaatt tgtgaccgcc gccaaacagg cccgggtggc gaaagcggct
      301 ctcgatttcg tcaaagcccg gggcctgtcc gaccgcccgc tccgtttcga cgtggtcggt
      361 ctattggggg aggaaatcgc ccatcttccc aacgccttcg ttcccgaggc gggacgctac
      421 cttttttaac ggttagtcgt ccgcggcccg gagcaccggc ggaacgggcg gggaaaggac
      481 accgtgcttt ttggccaggg ccgcccgggc ccggcccacg gtgccctttc cgtaacgcgc
      541 gttcaaccgg tccagggttt ccgccagctt ccgggggcgc tcggattcct tgaaaagcga
      601 gggttggccg ccatcgggct ccaacctagc caggcacacg ccgaccagcc ggacgggttt
      661 ttccagacgc tccttctccg tcaaaagacc gacggcggcg gcgaaaatgg cgtcgtcggt
      721 gaaaatcgcc tcgcccaaag cccggtggtg agtgcgcgtt ttaaaatccg cgtcccgcac
      781 cgtgagaacc acttcccgtc ccgccaatcg ataacgccgg gcccgggcgg ccaccttttc
      841 cgacaaaaac atgacatagg ctttcagcac gtcggggtcc cgggtgttcg cgggaaaggt
      901 cgtggcgtgc ccgacggatt tgaccgtgtc cacggcgtcc acccgggaca cgggcgcgtc
      961 gtcctggccc cggcccatgc gtttcagcca atggccccag gcgccgaacc gcgtgtaaag
     1021 cgtttcaaaa tccatggccg ccaactgccc gcagg
//
LOCUS       DAOQLS010000852         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_136744_length_1052_cov_3.471414, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000852 DAOQLS010000000
VERSION     DAOQLS010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136744_length_1052_cov_3.471414"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(439..909)
                     /locus_tag="PK362_12010"
     CDS             complement(439..909)
                     /locus_tag="PK362_12010"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNC75595.1"
                     /translation="MTQRTFRLIYALAALAFLVAGAAATRYVLARTALHECPFCQAAA
                     PLQDWSRRTDLTPVQKERLSSLESSYQKGTNGLLSRLSEEQTRLCSLLETAPADSPTV
                     DEQVDRVAAIRKEQDRLAAHHLLDVREVLTPDQRAGLFSAMKKGVCQPCELPGE"
BASE COUNT          204 a          324 c          323 g          201 t
ORIGIN      
        1 ccccgccgcg aaacccgccg tcagaacggc ggccatcaat tttttccaca tgatcttcct
       61 cctcattatt tttgcaacgc catcaaaacc atcatcacgg ccaagtccgc gccgcccatg
      121 atcatccaag gtatcgccga cttcttccac gggccgtctg cctgggggga gaattcccgg
      181 cgttcgagag gatgtcgccg ctttggggag cgtacatccg ctccacgtcc aaccggggct
      241 tgtccggcgt gtacgcctgg gatacccgcg ccttgaccgc atcccgtttt ttaagcgccg
      301 cccggatttc ggggttttcc ttccgagcga tggaaagcgc ctcgtcgcgt gaaaggggcg
      361 gcggaggctc cgcctcggac gacaccgaaa cgagaacgaa gaagagaatc ccgaacagcg
      421 accgagcttt cacaaaagtc attccccggg gagttcgcat ggttggcata ctcccttttt
      481 catggcggag aaaagccccg cccgttggtc gggagtgagg acctcgcgga cgtccaacag
      541 gtgatgggcc gcgagccggt cctgttcttt ccggatggcg gcgacgcggt ccacctgttc
      601 gtcgaccgtc ggggaatcgg cgggtgcggt ctcaagcagc gagcaaagcc gcgtctgctc
      661 ctccgataaa cggctcagta ggccgttggt tcccttttga taggatgatt ccaatgacga
      721 cagccgctct ttttgaaccg gtgtcaggtc cgttcgccgc gaccagtctt ggaggggcgc
      781 ggcggcctgg caaaaggggc attcgtgaag ggctgtccgg gcaaggacgt agcgggtcgc
      841 ggccgctccc gcaaccagga aggcgagggc cgccaaagca tagatgagtc gaaaagtccg
      901 ttgggtcatt gtccgctcca gatttgctca attgatcccg cctcgtgcgg ggaagtcagg
      961 gtcataacag tattttcaag gggaagcgcg cggttacgca acgccagcgc accgacggtc
     1021 acgcccaaga accagacagc cgcgaggccc gc
//
LOCUS       DAOQLS010000853         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_136977_length_1051_cov_4.561245, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000853 DAOQLS010000000
VERSION     DAOQLS010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136977_length_1051_cov_4.561245"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1012
                     /gene="hemB"
                     /locus_tag="PK362_12015"
     CDS             23..1012
                     /gene="hemB"
                     /locus_tag="PK362_12015"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNC75596.1"
                     /translation="MAFPATRLRRLRTTAAARRLVRETRLSPADLVWPLFVRSGRGLR
                     RPVSSMPGVFQLSVDQAVKDAREAARLGVPGVLLFGVPDRKDPLGKEAYAADGIVQRA
                     VRALKDALPDLLVATDLCLCEYTDHGHCGVVRKKGAEAVIDNDATLDLLAETARAQAE
                     AGADWVAPSGMMDGAVAALRRALDAAGRTHVGVLAYAVKYASAFYGPFRDAAESAPAF
                     GDRRTHQMDPANAREALREAALDLNEGADVLMVKPALPYLDILRRLRERFDVPLAAYN
                     VSGEYAMVKAAAAKGWIDEKRTVLEILTSLKRAGADLLITYHARDAARALADE"
BASE COUNT          153 a          370 c          362 g          166 t
ORIGIN      
        1 ctggttcttc cgaaataaaa caatggcgtt tccggccacc cgtctccgcc gccttcgaac
       61 gaccgccgcc gcccgccgcc tggtccggga aacccggttg tcgccggccg atctcgtttg
      121 gcccctcttc gtccgttccg gccggggcct tcgccgaccg gtgtcgtcca tgcccggggt
      181 gttccagctt tccgtggatc aggcggtgaa ggacgcccgg gaggcggcgc gcctgggcgt
      241 tcccggggtc ctgctcttcg gcgtgcccga tcggaaggac cctttgggga aagaggccta
      301 cgccgcggac ggcatcgtgc aacgggccgt gcgggccctc aaggacgccc tgccggacct
      361 tttggtggcg accgacttgt gcctctgcga atacaccgac cacggccact gcggcgtggt
      421 ccggaagaag ggcgcggagg ccgtgatcga caacgacgcg acgcttgacc tattggccga
      481 aacggcccgg gcccaggcgg aagccggggc cgactgggtg gcgccctcgg gcatgatgga
      541 cggggcggtg gcggcccttc gccgtgccct ggacgccgcc gggcggaccc acgtgggcgt
      601 cttggcctac gcggtgaaat acgcttcggc gttttacggc cctttccgcg acgcggcgga
      661 atccgcgccc gctttcggcg accggcgcac ccaccaaatg gatcccgcca acgcccgcga
      721 agcgcttcgg gaggccgccc tggatttgaa cgagggcgcg gacgtcctga tggtcaaacc
      781 ggccctgccc tatctggaca ttctccgccg cctgcgcgaa cggttcgacg ttccgctggc
      841 ggcctacaac gtgtcgggcg agtacgccat ggtcaaggcc gcggcggcca agggatggat
      901 cgacgaaaaa cgcacggtgt tggagatttt gacgtcgctg aaacgcgccg gggccgatct
      961 gctgatcacc taccacgccc gggacgccgc ccgcgccctg gcggacgaat gaaccgcccc
     1021 ccccgttcaa gcgcccgacg ccggcgcggg g
//
LOCUS       DAOQLS010000854         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_137040_length_1051_cov_2.797189, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000854 DAOQLS010000000
VERSION     DAOQLS010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137040_length_1051_cov_2.797189"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PK362_12020"
     CDS             complement(<1..638)
                     /locus_tag="PK362_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNC75597.1"
                     /translation="MKIKVDIFSDAICPWCYVGKKRLEKAMAVLTPEHAFEVRWHPFQ
                     LNPQTPAEGVDRAAYLARKFGGPDRLREMDAKMKEVGRGEGIEFKQEKILKTPNTLNA
                     HRLIAWSAQAGKQDALVENLFRACFSEGRDIGDTAVLARVAGESGMSASEAAAFLAGE
                     EGRAEVLKAEEEIKRAGVRGVPFFILNGRAFLEGAEPPEAFIQAVGDLSGVQP"
     gene            complement(710..>1051)
                     /locus_tag="PK362_12025"
     CDS             complement(710..>1051)
                     /locus_tag="PK362_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75598.1"
                     /translation="VLGGVARSLWKEHQEGEQRLQEAVDALSPLLQASYAGQMKGRRK
                     SLAVAYVLGVLGGLHHVYLGSLTRFLLFQFTAGGLLIWWTWDLGTMYFIVSAHNRREA
                     FKILQNLSSLP"
BASE COUNT          203 a          327 c          306 g          215 t
ORIGIN      
        1 ggttggacgc cgcttaaatc cccgaccgcc tggataaagg cctcgggcgg ctccgcccct
       61 tccagaaaag cccgcccgtt caggataaag aacggcaccc cccggacccc cgcgcgcttg
      121 atctcctcct ccgcttttaa cacctcggct cggccttctt cgccggccag aaaggcggcg
      181 gcttccgacg cgctcatacc gctctccccg gccacgcggg ccaggacggc cgtgtcgccg
      241 atgtcgcggc cttcggagaa gcaggcacgg aagagatttt cgaccagggc gtcctgcttc
      301 ccggcctggg cggaccaggc aatcaggcgg tgggcgttca gcgtgttggg ggttttgagg
      361 atcttctcct gcttgaattc gattccttcg ccgcgaccga cttccttcat tttcgcgtcc
      421 atttcccgca gccggtccgg gccgccgaat ttccgggcca gatacgccgc ccggtccacg
      481 ccctccgccg gcgtttgggg gttcagttga aaggggtgcc accgaacttc aaaggcgtgc
      541 tccggcgtta ggaccgccat ggccttttcc agtctttttt tgcccacgta gcaccaggga
      601 caaatggcgt cggaaaaaat gtccaccttg attttcatgg tcctctcccg gaaaagcgtt
      661 gcgttcccat tatgacaaca aacggccggt ggaatggttc cgacggtttc taggggaggg
      721 acgacagatt ttggaggatt ttaaaggcct cccggcggtt gtgggcgctg acgataaaat
      781 acatggtccc caaatcccat gtccaccaga tcaataatcc gcccgcggtg aattgaaata
      841 ggagaaacct ggtcaaggag cccaaataaa cgtgatgaag accgcccaac accccgagga
      901 cataagcgac cgccaaggat ttccgtcggc ctttcatttg gccggcgtaa gacgcttgaa
      961 ggagggggga gagggcgtcg acggcttctt gaaggcgttg ttcgccttct tggtgctctt
     1021 tccacaagga acgggcaacg cccccgagga c
//
LOCUS       DAOQLS010000855         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_137813_length_1048_cov_3.499496, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000855 DAOQLS010000000
VERSION     DAOQLS010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137813_length_1048_cov_3.499496"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1048
                     /locus_tag="PK362_12030"
     CDS             <1..>1048
                     /locus_tag="PK362_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75599.1"
                     /translation="GDPFIRLSRDRTTLRLRLPRAAFGNSADFESAAAPMLDALDAVL
                     ASANGGRVLGFPLRHARREDFPDRVLNRFLDEGAGRGIDLLMEVGSFFKADFLRSVFS
                     REPEAYARKLFGSNPADQKNPLSLSDFRGDSSRIGWFEELLARHRAPLASEYARIDQA
                     IRERRWSDIAGRLGGGGFSEALFAWIYDHPNQANLAREAVQADVELMHWQAEWLRSVS
                     WRQGPWAIPADVSARAAVELAVRRLTEIHWLEAKRVFLRNARLRAWAAATPIDPYRRA
                     VFPVGALHRPGLGTESFEWVDPPPAGTEGGGTDAASLSALLSLPENRLMEVLGNRLAS
                     AVPPPSEADLAPFSP"
BASE COUNT          149 a          328 c          361 g          210 t
ORIGIN      
        1 aggcgatccc ttcatccggt tgtcccggga tcggacgacc cttcggcttc gcctgccgcg
       61 cgccgccttc ggcaattcgg ccgatttcga atcggccgcg gccccgatgc tggacgccct
      121 ggacgccgtc ttggcctcgg ccaacggcgg acgtgtgctg gggttcccgc ttcggcacgc
      181 ccggcgggag gattttccgg accgggtctt gaaccgattc ctcgacgagg gcgccgggcg
      241 gggcatcgac cttctgatgg aggtgggctc cttttttaag gcggattttc tccgatcggt
      301 gttttcaagg gagcccgagg cctacgcccg taaattgttc gggtcgaacc ccgccgatca
      361 aaaaaatccc ctgagtttgt ccgatttccg gggagattcc tctcggatcg ggtggttcga
      421 ggaacttttg gcccgacatc gagccccctt ggcctcggaa tacgcccgga ttgaccaagc
      481 gatccgggaa cggcgctggt cggacatcgc ggggcggctg gggggcgggg gtttttcgga
      541 agcgctgttc gcctggattt acgatcatcc gaatcaagcc aacctcgccc gggaggcggt
      601 ccaggcggac gtggaattga tgcattggca ggcggagtgg cttcgaagcg tgtcctggcg
      661 ccaggggcct tgggcgattc ccgcggatgt ttccgcgcgg gccgccgtgg aacttgcggt
      721 tcggcggttg accgaaattc attggctgga agcgaaacgc gttttccttc gaaacgcccg
      781 gttgagggcc tgggcggcgg cgacccccat cgacccctat cggcgcgcgg ttttccccgt
      841 gggggcgctc caccgaccgg gactggggac ggaatccttt gaatgggtgg acccgccccc
      901 ggccggaacg gagggcggcg gaaccgacgc cgcgagcctc agcgctcttc tgtcgttgcc
      961 cgaaaaccgt ctgatggagg ttctggggaa ccgattggcg tccgccgtgc cgcctccttc
     1021 cgaggcggac ctggcgccct tttcgccc
//
LOCUS       DAOQLS010000856         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_138029_length_1047_cov_7.227823, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000856 DAOQLS010000000
VERSION     DAOQLS010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138029_length_1047_cov_7.227823"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PK362_12035"
     CDS             complement(<1..133)
                     /locus_tag="PK362_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNC75600.1"
                     /translation="MTTVQAPPRIKTLVLVGNPNVGKSVLFHRLTGRYATVSNYPGTT
                     "
     gene            complement(171..869)
                     /locus_tag="PK362_12040"
     CDS             complement(171..869)
                     /locus_tag="PK362_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75601.1"
                     /translation="MNKKWTMGAIVLMAGVGARAHVRDYLDTYSPATLEKGAAEMELW
                     TDLRNPDQGDAYWVHQTEVEYGVTNRYTLGLYGVFVDGQGFSALKVENRYRPEMPASW
                     PLDTALYLELKKANGRKDENEIEGKILLGKTWGAVNVTANPILALEESRDANGDTAWE
                     LESGLAVGTCYRHAWKNVTPGVELLIVENQTRLTPGVYIDVMPGIRFNVGVGIGLEKK
                     ADDAQLKSILEVEF"
     gene            complement(866..>1047)
                     /locus_tag="PK362_12045"
     CDS             complement(866..>1047)
                     /locus_tag="PK362_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75602.1"
                     /translation="LAGRWSAAREGFSELMNLQREAHRVFRPTLFQRVGRWFKKKPAG
                     PADHVGNDSQGAQQP"
BASE COUNT          181 a          336 c          313 g          217 t
ORIGIN      
        1 ccgtcgtgcc cgggtaattg gacacggtgg cgtaccgccc cgtcaaccgg tgaaaaagca
       61 ccgatttccc gacgttgggg ttccccacga ggaccagcgt cttgatccgc gggggggcct
      121 gaaccgtcgt cacgggcggc gacctccggc ggaggaccgg cgcgcggaaa tcagaattcc
      181 acctcgagaa tggatttcaa ctgggcgtcg tcggcctttt tctcaagccc gatgccgacg
      241 ccgacgttga accggatgcc cggcatcacg tcgatgtaga cgccgggggt caggcgcgtt
      301 tgattttcga caatcaacag ctcgacgccc ggcgtgacgt tcttccaggc gtggcggtaa
      361 caagtgccga cggccaggcc cgactccaat tcccaggcgg tgtcgccgtt ggcgtcccgc
      421 gactcctcca acgcgaggat cgggttcgcg gtcacgttca cggcgcccca ggtttttccc
      481 aacaatattt tcccctcgat ttcgttttcg tccttgcggc cgttggcttt cttcaattcc
      541 agataaaggg ccgtgtccag gggccaggag gcgggcatct ccggccggta gcgattttcg
      601 accttcaggg cggaaaagcc ctggccgtcg acaaacacac cgtaaaggcc cagggtgtag
      661 cggttcgtca cgccgtattc gacttcggtt tggtggaccc aataggcgtc gccctggtcc
      721 gggttgcgca aatccgtcca cagctccatt tcggccgcgc ccttctccag ggtcgccggc
      781 gaataggtgt ccaggtaatc ccgcacgtgc gcccgggccc cgactccggc cattaaaacg
      841 atcgctccca tcgtccactt tttattcatg gttgttgcgc tccttgtgag tcgtttccga
      901 catggtccgc cggtccggcg ggtttctttt taaaccaccg gccgacgcgt tgaaaaagag
      961 tggggcggaa aacccggtgg gcttcccgtt gaaggttcat caattccgaa aaaccctccc
     1021 gggcggcgga ccaccgtccg gccagca
//
LOCUS       DAOQLS010000857         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_138065_length_1047_cov_3.781250, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000857 DAOQLS010000000
VERSION     DAOQLS010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138065_length_1047_cov_3.781250"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..628)
                     /locus_tag="PK362_12050"
     CDS             complement(23..628)
                     /locus_tag="PK362_12050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HNC75603.1"
                     /translation="MIARLRGPVVEKTAGAVVVDAGGVGYEVAVGSGTAARLPDLGEE
                     VRLFVVESVAMYGGGTSLYGFLTEGEKRLFNAFRDLVPGTGAKKALELLDKAAASLAD
                     FYRAVADKNAGALVSGFGFTAKTAEKLVAALHGKLDAAPAAGSPVRGSSALEEAVAGL
                     IALGYREPAARAAAQTAQLGGAGGAAQDLIRESLRHLSGRN"
     gene            complement(625..>1047)
                     /locus_tag="PK362_12055"
     CDS             complement(625..>1047)
                     /locus_tag="PK362_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNC75604.1"
                     /translation="GVILTPAKTPLADRLCRIARELRALVARVQPDAAAIEELFFAKE
                     SRSAAIVGHGRGVLLLALAELGLPIHEYNPKQVKVALTGHGGAEKFQMQNMIQRLLGL
                     PEIPKPDDAADALAIALCHLNTFVPPAALAGPAAGRPR"
BASE COUNT          169 a          355 c          364 g          159 t
ORIGIN      
        1 cggggcgaaa gtcgtccacg gatcaattcc ttcccgacag gtgccggagg gattcgcgaa
       61 tcaaatcctg ggccgcgccg cccgccccgc ccaactgggc cgtctgggcc gccgcccggg
      121 ccgccggctc ccggtacccc agggcgatca aaccggccac ggcctcctcc agcgcgctcg
      181 agccccggac gggggagccg gcggccggcg ccgcgtccaa tttgccgtga agcgcggcca
      241 ccagtttttc ggcggttttg gccgtaaagc cgaagccgga caccagggcg ccggcgttct
      301 tatcggccac ggcgcggtaa aaatcggcga gcgaggcggc cgctttgtcc aacaattcca
      361 gggctttctt ggccccggtg ccgggcacga ggtcccgaaa ggcgttaaaa agtcgctttt
      421 ccccttcggt caaaaacccg tacagggagg tgccgccgcc gtacatggcc accgattcga
      481 cgacgaacaa ccgaacctct tcgcccaaat ccggcaggcg ggccgcggtg ccggacccca
      541 cggccacctc gtagcccacg ccccccgcgt ccacgaccac ggcgccggcg gttttttcca
      601 cgacggggcc gcgaagacga gcgatcaccg gggcctcccg gcggcggggc ccgccagggc
      661 ggccgggggc acgaaggtgt tcaaatggca gagggcgatg gcgagcgcgt cggccgcgtc
      721 gtcgggtttg gggatttccg gaagccccag gaggcgttgg atcatgtttt gcatttgaaa
      781 tttttcggcg ccgccgtggc cggtgagggc gactttgacc tgcttggggt tgtattcgtg
      841 gatgggcagc ccgagctccg ccagggccag gagcaacacc ccgcggccgt ggccgacgat
      901 ggcggcgctc cggctttcct tggcgaaaaa aagctcttca atggcggcgg cgtcgggttg
      961 aacccgggcc accaaggccc gcaactcccg ggcgatgcgg caaagccggt cggccagggg
     1021 ggttttggcg ggggtcagaa tgacccc
//
LOCUS       DAOQLS010000858         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_138105_length_1047_cov_3.147177, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000858 DAOQLS010000000
VERSION     DAOQLS010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138105_length_1047_cov_3.147177"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..644
                     /locus_tag="PK362_12060"
     CDS             111..644
                     /locus_tag="PK362_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNC75605.1"
                     /translation="MTLIGKKVAILLERDFQDLEVWYPALRLKEEGATVVFVGTTGKD
                     YKGKFGYPATADVLAADVRAADFDAVVVPGGWAPDFLRRHESVLSFVRDLHAAGKPVA
                     AICHAGWVLASAGILKGKTATSFSAIKDDVINAGARWVDREVAVDGALITARTPDDLP
                     AFCRELISQVARVPAAH"
     gene            649..963
                     /locus_tag="PK362_12065"
     CDS             649..963
                     /locus_tag="PK362_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNC75606.1"
                     /translation="MADVKVCAVADLPPGEARVVTLRDKEVAVFNVAGSFHAMDNLCP
                     HRGAPLSDGRLEGKTVICPWHGWQFDVTTGALVVSPDSRQTTYPVTVRDGHVYVAPRG
                     NA"
BASE COUNT          178 a          342 c          337 g          190 t
ORIGIN      
        1 ggtggaacgc ggcgggaacc ccgacgagcg ctcccggggc caccggcgcc gtcgttcgaa
       61 cttttttaaa ttaccttcgg tccctgtaaa acttttcggg aggcgacgac gtgacgctca
      121 taggcaaaaa agtggccatc ctgctggaac gggattttca ggatttggaa gtgtggtatc
      181 cggcccttcg gctgaaggaa gaaggagcca ccgtcgtgtt cgtcggcacc acgggcaagg
      241 attacaaagg gaaattcggc taccccgcca cggccgacgt cctggcggcg gatgtccgcg
      301 cggcggattt cgacgcggtg gtcgtcccgg gcgggtgggc accggatttt ctccggcggc
      361 acgaatccgt cctgtctttc gtgcgcgact tgcacgcggc cggcaaaccg gtggcggcca
      421 tttgccacgc cggttgggtg ctcgcctccg ccggaattct gaaaggcaaa acggcgacct
      481 ccttttccgc catcaaagac gacgtgatca acgccggcgc ccgctgggtg gaccgggaag
      541 tggccgtcga cggggccttg atcacggccc gaacccccga tgacctgccc gccttttgcc
      601 gtgaactcat ttcccaagtg gcccgcgtcc cggccgccca ctgatcccgt ggccgacgtc
      661 aaagtgtgcg ccgtcgccga tttgcccccc ggggaggccc gcgtggtgac tcttcgggat
      721 aaggaggtgg ccgttttcaa cgtcgccgga tcttttcacg ccatggacaa tctctgcccc
      781 caccgggggg cgcccctgag cgacggccgc ttggagggga aaacggtgat ttgcccctgg
      841 cacggatggc aattcgacgt cacgacgggg gccctcgtgg tcagccccga cagtcgccaa
      901 accacctacc cggtgacggt tcgcgacgga cacgtctacg tcgccccgcg ggggaacgcg
      961 tgagccggct tcgaaccctt tccgtcgccc acagccccga cccggacgac gctttcatgt
     1021 tttacgggat cgccaagggg aaagtgc
//
LOCUS       DAOQLS010000859         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_138223_length_1047_cov_2.050403, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000859 DAOQLS010000000
VERSION     DAOQLS010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138223_length_1047_cov_2.050403"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /gene="atpH"
                     /locus_tag="PK362_12070"
     CDS             <1..558
                     /gene="atpH"
                     /locus_tag="PK362_12070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNC75607.1"
                     /translation="VVLKLQDRPVAARYARALFAAASSQKAVEPVRRELAQLRAALAA
                     SPALDDALKHPRIAPEVKQQVIEKVFGRFSPVLNRFIGVLLEKKRWDALRDIFPVYER
                     LADAAGGILSVRAETALPLGDDETANVRRSLEKSWGGPVSLKTRVREDLLGGLVLHVG
                     DLVLDNSLKGQLERLREVWLAGTSR"
     gene            580..>1047
                     /locus_tag="PK362_12075"
     CDS             580..>1047
                     /locus_tag="PK362_12075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938952.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC75608.1"
                     /translation="MSIQPEEITRILKERLEGVALSTEMKEVGSVLQVGDGIARVYGL
                     GNAVAGEMLLFPHGVVGIVLNLERESVGAVLLGEATLIKEGDEVRRTGRIMEVPVGDG
                     MIGRVVNALGAPIDGKGPIKSAKARPVEVVAPGVVERQPVREPLQTGIKAIDAM"
BASE COUNT          182 a          302 c          367 g          196 t
ORIGIN      
        1 gtcgtgctga aacttcaaga tcgtcccgtg gccgcccgct acgcccgggc gcttttcgcg
       61 gccgcgtctt ctcagaaagc ggtggaaccc gtccgccggg agctcgcgca gttgcgtgcg
      121 gccttggccg cgtccccggc tttggacgac gcgttgaagc atccgcggat tgcgcccgag
      181 gtgaaacagc aggtgattga aaaggtcttc ggtcgttttt ctccggtctt gaaccggttc
      241 atcggggtgc ttctggaaaa gaagcggtgg gacgccctgc gggacatctt tcccgtctat
      301 gagcggctgg ccgatgcggc gggtggcatt ctgtccgtcc gggcggagac ggccctgccc
      361 ctcggggacg acgaaaccgc gaacgtgcgc cgctcgctgg agaagagttg gggcggtccg
      421 gtgtcgctga aaacccgggt gcgggaggat ttgttgggcg ggctcgtcct tcacgtgggc
      481 gacctcgtgt tggacaatag cttgaaaggc cagttggagc gcctgcgcga ggtatggctc
      541 gcggggacgt ctcgataaaa ttaaaaggac cctcacgcca tgtcgattca acctgaagag
      601 atcacgcgca ttttgaaaga acgtctcgag ggcgtcgccc tttcgacgga aatgaaagag
      661 gtgggctccg tccttcaagt gggcgacggc atcgcccgcg tctacggttt gggcaacgcc
      721 gtggccggcg aaatgctgtt gttcccccac ggcgtggtgg gcatcgtttt gaatttggag
      781 cgggaaagcg tgggcgccgt tctgttgggc gaggcgacgc tcattaagga aggcgacgag
      841 gtccgccgca cgggccggat catggaagtt cccgtggggg acggcatgat cggccgcgtg
      901 gtgaacgcgc tgggcgcgcc catcgacggc aaaggcccca tcaaatccgc caaagcccgg
      961 cccgtggaag tcgtggcccc cggcgtggtg gaacgccaac cggtgcgcga gcctctgcaa
     1021 accggcatca aagccatcga cgccatg
//
LOCUS       DAOQLS010000860         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_138339_length_1046_cov_4.857719, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000860 DAOQLS010000000
VERSION     DAOQLS010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138339_length_1046_cov_4.857719"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1046)
                     /locus_tag="PK362_12080"
     CDS             complement(<1..>1046)
                     /locus_tag="PK362_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC75609.1"
                     /translation="RRPVGTGPYKFLEWKPDEKIVLGANADYFEGRPRLDRIVYRIIP
                     DSSVEFLELRNESIDFMGLSPDQAAAYPEFFRHYNKFSYPSFSYTYLAFNLDHPFFAD
                     RRVRRALAQAVDRKDIIQGVLLGRGRSATGPFPPSSWAFDASVPEDVYDPEAARGLLK
                     SAGWSDTDGDGILDKDGRPFEFTVLTNQGNKMRELTATILQSHFARVGIKMNIRILEW
                     SAFIHNYVDKRNFDALILAWNLSRDPDQYVIWHSSQRGPGKYNFAGYANPAADRLWEE
                     GRQTFDPDRRRDLYHRLHRMLADDRPYLFLYYPESLPVVHRRIHGVQTAPAGIAWNFR
                     EWFVPRALQKYRGT"
BASE COUNT          195 a          318 c          343 g          190 t
ORIGIN      
        1 cgtcccccgg tatttttgaa gggcccgggg cacgaaccat tcccggaaat tccaggcgat
       61 gccggccggc gccgtttgaa ccccgtggat ccgtcgatgc accacgggca gggattccgg
      121 atagtacagg aacaaatagg ggcggtcgtc ggccagcatc cggtggagtc ggtggtaaag
      181 gtcccgccgg cggtcggggt cgaaggtttg gcggccctcc tcccacaggc ggtccgccgc
      241 cgggttcgcg taacccgcaa aattgtattt gccgggcccc cgttggctgg aatgccagat
      301 gacgtattga tccgggtccc gggagaggtt ccaggcgagg atcaaagcgt cgaaatttcg
      361 cttgtccacg taattgtgga tgaaggccga ccattccaaa atccggatgt tcattttgat
      421 ccccacccgg gcgaaatggg actgaaggat ggtcgcggtc agttcgcgca tcttgttccc
      481 ctggttggtc agcacggtga actcgaaggg gcggccgtct ttgtccaaaa tcccgtcgcc
      541 gtcggtgtcg gaccagccgg cggatttaag cagcccccgg gcggcctccg gatcgtagac
      601 gtcctcgggc accgacgcat cgaaggccca ggagctgggc ggaaagggcc ccgtggccga
      661 gcggccccgc ccgagcaaca ccccctgaat gatgtccttc cgatccaccg cctgggccag
      721 ggcccggcgc acccgccggt cggcgaagaa cgggtggtcc agattaaagg ccaggtacgt
      781 gtaggaaaag gacggatagc tgaacttgtt gtagtgccgg aaaaattccg ggtaggccgc
      841 ggcctggtcc ggcgagagcc ccatgaaatc gatcgattcg ttccgcaact ccaaaaactc
      901 cacggagctg tcggggatga tccggtagac gatccggtcc agccggggcc gcccctcgaa
      961 atagtccgcg ttggccccga ggacgatctt ttcgtcgggc ttccactcca ggaatttgta
     1021 gggtcccgtg cccacgggcc gccggt
//
LOCUS       DAOQLS010000861         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_138404_length_1046_cov_2.980827, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000861 DAOQLS010000000
VERSION     DAOQLS010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138404_length_1046_cov_2.980827"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..>1046)
                     /locus_tag="PK362_12085"
     CDS             complement(85..>1046)
                     /locus_tag="PK362_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC75610.1"
                     /translation="REKLRMGLIAHAGDGNLHPNLIFDERDPLETQRVKAAGHDMLRV
                     CVELGGSISGEHGIGSDKREAMRWLFTPDTLGLFRRIKTALDPAGLCNPDKLLPVAEP
                     SAPPVEIRGAESEALGLRIESVEQAQKWLRRAAAEKRPLTIAGHKKRLAGDVRGPLLD
                     LTGLAAVVRHDPANFTLTVQAGARIPDVHRFLAATGQHLYVFGEGTVGGVVSRNAGGG
                     PPLRDQILGMTVALPGGDLAVLGAPVVKNVAGYDAAKLLIGAWGSLGVILDLTFRLHP
                     VPAPSARASDPPRPAFWNVPALGTVKKAFDPDNLLNPVLFERP"
BASE COUNT          177 a          337 c          361 g          171 t
ORIGIN      
        1 cgtgcagaag ccgcaccggt tgcagtggag cgtatcgtcg taggaccgct ttggtcgtga
       61 ccagatcggg cgcggggcgg tcggtcatgg gcgctcgaac aacacggggt tcagcaaatt
      121 gtccgggtcg aaggctttct taacggtccc aagggcgggg acgttccaaa acgcggggcg
      181 cggaggatcc gacgcccggg ccgacggggc gggaaccggg tgcagccgga aggtgaggtc
      241 cagaatcacc cccagcgatc cccaggcgcc gatcaacaac ttggcggcgt cgtagcccgc
      301 gacgtttttc acgacggggg ccccgaggac cgcgaggtct cccccgggga gggcgacggt
      361 cattcccaaa atttgatcgc gcagaggcgg accgccgccg gcgttccgag atacgacgcc
      421 gcccacggtt ccctcgccaa acacgtacag gtgctgtccc gtggcggcga ggaaacgatg
      481 cacgtccggg atccgggccc ccgcctgcac cgtcaaggta aagttggccg ggtcgtggcg
      541 gaccaccgcc gcgaggcccg tcaggtccaa cagcgggccg cggacgtcgc cggccagccg
      601 ttttttgtga ccggcaatcg tgagggggcg tttttccgcc gccgcccggc ggagccactt
      661 ttgcgcctgt tcgaccgatt cgatccgaag gcccaacgct tcgctttcgg cgccgcggat
      721 ttcaacgggc ggggcggagg gttccgccac cgggaggagt ttgtcgggat tgcagaggcc
      781 cgccggatcg agggcggttt taattcggcg gaaaagtccc agcgtgtccg gcgtgaacag
      841 ccaccgcatg gcctcccgtt tatcggaacc gatcccgtgc tcgccggaaa tggagccgcc
      901 caactccacg cacacgcgaa gcatgtcgtg cccggcggcc ttgacccgtt gggtctccag
      961 ggggtcccgc tcgtcgaaaa tcaaattggg atgcaagttc ccgtccccgg cgtgggcgat
     1021 gagacccatc cgcaattttt cccggt
//
LOCUS       DAOQLS010000862         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_138876_length_1044_cov_4.037412, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000862 DAOQLS010000000
VERSION     DAOQLS010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138876_length_1044_cov_4.037412"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK362_12090"
     CDS             <1..288
                     /locus_tag="PK362_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75611.1"
                     /translation="MVDAGAGDWLGARLQWKNDRQSLQIVKTVPLPAAPRVRLGPLTN
                     YTLSAGMNQSLRLAWNGSTCGCQLQKIETTARQLARAREALKALESPDNRR"
     gene            360..758
                     /locus_tag="PK362_12095"
     CDS             360..758
                     /locus_tag="PK362_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75612.1"
                     /translation="MGAGSARLPDNADSFGTLFAHGFVFLAAVAGLFIGLALTAGIGR
                     ATEKALCRLGAPVAAAPSLAILVTGLALWRIAGFVPIAVPGIRSGERSGERGGPRPRI
                     ESPPPFREDPCRQPPPENPRERKRWEEECR"
     gene            complement(783..>1044)
                     /locus_tag="PK362_12100"
     CDS             complement(783..>1044)
                     /locus_tag="PK362_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNC75613.1"
                     /translation="VKAFNAVGNPHMVDPSFPGGRPTMFIGGNDAGAKKTVMGLLTDF
                     GWESVDLGGIETSRYLEPLAMIWILHYLRTKSGAHAFKLLKK"
BASE COUNT          181 a          336 c          334 g          193 t
ORIGIN      
        1 atggtggacg cgggggcggg ggattggctc ggcgcccgcc ttcaatggaa aaacgaccgg
       61 cagtcgcttc aaatcgtcaa aaccgtccct ttgcccgccg ccccccgggt tcgtcttggg
      121 cccctgacga actacacgtt gagcgcgggg atgaaccagt cccttcggct cgcctggaac
      181 gggtcgacgt gcggttgcca attgcaaaaa attgaaacaa cggcccggca actggcccgg
      241 gcccgggaag cccttaaagc cttggagtcc ccggacaaca ggcgatgata aacggcgaga
      301 agctccgatc ggttctccgg tccagcggac ttttttgcgg gggactctgg ggcgcttggg
      361 tgggcgccgg atccgcccga cttccggaca acgcggacag ctttgggact ctcttcgccc
      421 atggatttgt ttttttggcc gccgtcgccg gccttttcat cggcttggcc ctcacggccg
      481 ggatcgggag agcgaccgaa aaagctcttt gccggctggg ggcccccgtc gccgccgccc
      541 cgtcgctggc gatcctggtg acgggcttgg ccctgtggcg gattgcggga tttgtcccca
      601 tcgcggtccc cggaattcgc tcgggcgaac ggtcgggaga gcgcggcgga ccgcgcccgc
      661 gaatcgaatc gccccctcct tttcgggaag acccctgccg ccaacctccc ccggaaaatc
      721 cccgggaacg gaaacggtgg gaggaggaat gccgctaagg ggggcgcggg cggccccccc
      781 cactattttt ttagcaactt aaacgcgtgg gcgccggact tcgtccgaag gtaatgcaaa
      841 atccagatca tggccagggg ttcgaggtag cgcgacgttt caattccgcc caaatccacg
      901 ctctcccagc cgaagtcggt caacaatccc atcaccgttt ttttagcgcc ggcgtcgttg
      961 ccgccgatga acatcgtggg gcgccccccg ggaaacgacg ggtccaccat gtgggggttg
     1021 ccgacggcgt taaacgcttt gacc
//
LOCUS       DAOQLS010000863         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_138937_length_1044_cov_2.879676, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000863 DAOQLS010000000
VERSION     DAOQLS010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138937_length_1044_cov_2.879676"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK362_12105"
     CDS             complement(<1..444)
                     /locus_tag="PK362_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC75614.1"
                     /translation="MGRPFRPFVLLPFLLAACGRDPIPEPAWEGAATFHPGDVGDTFV
                     ESSLGDASRLNPLLATDSASGDICGYVFNGLVKYDKDLRLVGDLAAAWDIRRGGLEIV
                     FHLRPGVRWHDGVPFTARDVVFTYRVLIDSNVATPFGADFADVKSV"
     gene            complement(450..>1044)
                     /locus_tag="PK362_12110"
     CDS             complement(450..>1044)
                     /locus_tag="PK362_12110"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNC75615.1"
                     /translation="APTAAGPVTYPAQSRAVEEKRKFEQGYIMLGFPAAAVTDARYPK
                     IKLINALLGAGMSSPLFTVVREQAGLAYEVSSFYASRSAGSAMVIYAGTDPKNVDLAE
                     KKIHSVLTDFVSRKPTEDELNDAKNLIQGHYLMDHQTNSRIAWYLGWWELLGKGLAYD
                     VQYPADIGAVTAEDLHREAQSLFARPSVTVRVRNGGR"
BASE COUNT          202 a          315 c          356 g          171 t
ORIGIN      
        1 cacgcttttc acgtcggcga aatccgcgcc gaagggcgtg gccacgttgg aatcgatgag
       61 cacccggtag gtgaagacca cgtcccgggc ggtgaaagga acgccgtcgt gccagcggac
      121 gccggggcgg agatggaaaa caatttccag accgcccctt cgaatgtccc aggcggccgc
      181 caagtccccc accaggcgga ggtccttgtc gtatttgacc aggccgttaa aaacgtagcc
      241 gcagatgtcg cccgaagccg agtcggtggc caacagaggg ttcaggcggg aggcgtcgcc
      301 gaggctggac tccacaaagg tgtcgccgac gtcgccgggg tggaaggtcg cggcgccctc
      361 ccaagccggt tcgggaatgg ggtcgcggcc gcaggccgcc aaaaggaaag gaagaaggac
      421 gaacggccgg aacggccgac ccacgaaaac taccgcccgc cgttccggac ccgcacggtg
      481 accgacgggc gggcgaacag ggactgggct tcccggtgca gatcctcggc ggtgacggcg
      541 ccgatgtcgg cggggtattg cacgtcgtag gccaggccct tgcccaggag ctcccaccag
      601 cccagatacc aggcgatgcg ggaattggtt tggtggtcca tcagatagtg gccttgaatc
      661 aaatttttgg cgtcgttcaa ttcgtcctcg gtgggtttcc gggaaacgaa atccgtcagg
      721 accgaatgaa ttttcttttc cgccaggtcc acgtttttgg ggtcggtccc ggcgtaaatg
      781 accatcgcgc tgccggccga acgggaggcg tagaaggagg acacttcgta ggccagcccc
      841 gcctgttccc gcacgacggt gaaaaggggc gagctcatgc ccgcccccaa aagcgcgttg
      901 atgagtttga ttttcggata gcgggcgtcg gtcacggcgg cggcgggaaa accgagcatg
      961 atgtaaccct gctcaaattt ccgcttttcc tccaccgccc gggactgggc cgggtaggtc
     1021 acggggcccg ccgccgtggg ggcc
//
LOCUS       DAOQLS010000864         1043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_139165_length_1043_cov_4.065789, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000864 DAOQLS010000000
VERSION     DAOQLS010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139165_length_1043_cov_4.065789"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK362_12115"
     CDS             <1..556
                     /locus_tag="PK362_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75616.1"
                     /translation="AGSEEDAKVDEARAVLRGIAADKMIRLAVPVYQDTPYTVRGVRV
                     SAGGAQAVGTLVEDVGAIAKQNLKDRIGRTRAKAIARAVVKYLITQKVSEKVEKKNNG
                     GAGRLVKALLQAAVAMTETADKRSWRTLPDQILMARLPLPPGAHDVTVDFLNGSGGVA
                     RTAALPGVKIAAGKRTYAMVRSAE"
     gene            580..>1043
                     /locus_tag="PK362_12120"
     CDS             580..>1043
                     /locus_tag="PK362_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNC75617.1"
                     /translation="MTKRFAARTLLALGLGGVALTAAAAKIPAWVRGPDRAYPESKYV
                     IGVGVGSDLDAARTNARAEISKTFQARIQQTMKDTQTEQSSAAGKRRGPALGTQKSEL
                     NTTVSTDNLLEGVAIKETWFDKKGKKYYALALLDKVTAQRSLSSQITDLEET"
BASE COUNT          222 a          333 c          340 g          148 t
ORIGIN      
        1 ggcgggcagc gaggaagacg ccaaggtgga cgaagcccgg gcggtcctcc ggggcatcgc
       61 ggccgacaaa atgatccgcc tggccgtgcc ggtctaccag gacacgccct acaccgttcg
      121 gggcgtccgg gtgtcggccg ggggcgccca agccgtcggg accttggtgg aggacgtggg
      181 ggccatcgcc aagcagaacc tgaaggaccg catcggccgc acccgggcca aggccatcgc
      241 ccgggccgtc gtcaaatatt tgatcaccca gaaagtgtcg gaaaaggtgg aaaagaaaaa
      301 caacggcggc gccggccggc tggtgaaggc gctcctccaa gccgccgtgg ccatgaccga
      361 gaccgccgac aaaaggtctt ggcggaccct gcccgatcag attttaatgg cgcgcctgcc
      421 cctccccccg ggggcccacg acgtgacggt ggattttctg aacgggtcgg ggggcgtcgc
      481 ccgcaccgcg gccctgccgg gggtgaaaat cgcggccggt aaacggacct acgcgatggt
      541 tcgatccgcg gaataatttt ctaagatgga ggaggattta tgacgaaacg cttcgccgca
      601 cgaacgttgc ttgcgttggg cttggggggg gtggctttaa cggccgccgc ggccaagatt
      661 cccgcctggg tgcgggggcc ggaccgcgcc tacccggaat ccaaatacgt gatcggcgtg
      721 ggggtcgggt cggacctgga cgccgcccgc accaacgccc gggccgagat ttccaaaacc
      781 tttcaagcgc gcattcagca aaccatgaag gacacccaga cggagcaatc ctccgccgcg
      841 ggcaaacggc ggggaccggc cctcggcacc caaaaaagcg agctcaacac gacggtctcc
      901 accgacaatt tgctggaggg cgtcgccatc aaggaaacct ggttcgacaa gaagggcaag
      961 aaatattacg ccctcgccct gttggacaaa gtcaccgccc aacgctccct gagctcccaa
     1021 atcaccgatc tcgaagaaac cat
//
LOCUS       DAOQLS010000865         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_139482_length_1042_cov_3.117528, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000865 DAOQLS010000000
VERSION     DAOQLS010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139482_length_1042_cov_3.117528"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..562)
                     /locus_tag="PK362_12125"
     CDS             complement(2..562)
                     /locus_tag="PK362_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNC75618.1"
                     /translation="MNTTTQANTLPFKIWTRGKVRDVYDLGENLLIVATDRISAYDFV
                     LPTPVPDKGKVLCQTSNFWFEKLKEVCPNHLVATSVADFPEEIRRHCAGMEGRTVLVK
                     KAKRVDIECVVRGYLAGSGWKEYQESQSVCGVKLPAGLVESSKLPAPIFTPATKAPDG
                     EHDENISFERMVSVVGILKGILGWFF"
     gene            complement(566..>1042)
                     /locus_tag="PK362_12130"
     CDS             complement(566..>1042)
                     /locus_tag="PK362_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HNC75619.1"
                     /translation="MHMTLVEPLRFLFKDEVRALGKELGLPDEILFRQPFPGPGLAVR
                     VLGDITEEKLRMARESDYIVEEEIRAAGLYNKLWQAFTVFLPVNSVGVMGDERTYQNA
                     VALRAVNSVDAMTADWARLPYDVLAKISTRIINEVRGINRVVYDISSKPPATIEWE"
BASE COUNT          226 a          316 c          274 g          226 t
ORIGIN      
        1 cctaaaaaaa ccatcccaaa atccctttca aaatccccac cacgctcacc atccgctcga
       61 acgagatgtt ttcgtcatgc tcgccgtcgg gcgctttggt ggccggggta aaaatgggcg
      121 cgggcagttt tgacgattcc acgagccccg ccggcagttt caccccgcag accgattggg
      181 attcttgata ttccttccag ccggacccgg ccagatagcc gcgcaccacg cattcgatgt
      241 ccacccgttt ggcttttttc accaaaaccg tccggccctc catccccgcg caatgacgcc
      301 gaatctcctc gggaaaatcc gccacggatg tggccaccag atggttgggg caaacctcct
      361 ttaatttctc aaaccaaaaa ttggaggttt ggcaaaggac ttttcctttg tccggcaccg
      421 gcgtcggcaa cacaaaatcg taggccgaga ttcggtccgt ggcaacaatg aggagatttt
      481 ctcccagatc gtaaacgtcc cgcacttttc cgcgggtcca gattttaaac ggaagcgtat
      541 tggcttgagt ggttgtattc ataggtcact cccattcgat ggtggcgggg ggtttggacg
      601 aaatgtcgta gaccacccgg ttgatccccc gcacttcgtt aatgatgcgg gtggaaattt
      661 tggccaacac gtcgtaggga agacgcgccc agtcggcggt catggcgtcg acggaattga
      721 cggcgcgaag cgccacggcg ttttggtagg tgcgctcatc gcccatgacg cccacgctgt
      781 taaccggcag aaacaccgta aaggcctgcc acagtttatt gtaaaggccc gccgcgcgaa
      841 tttcttcctc cacgatataa tccgattccc gggccatgcg cagtttctct tcggtaatgt
      901 cgcccaacac ccggaccgcc aggccgggac ctgggaacgg ctggcgaaac aggatctcgt
      961 ccggcaggcc cagctctttg cccagggccc gcacttcatc cttaaaaaga aatcgaaggg
     1021 gttccaccag ggtcatgtgc at
//
LOCUS       DAOQLS010000866         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_139641_length_1042_cov_1.501520, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000866 DAOQLS010000000
VERSION     DAOQLS010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139641_length_1042_cov_1.501520"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PK362_12135"
     CDS             <1..778
                     /locus_tag="PK362_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC75620.1"
                     /translation="LLVAGTGVLIYLDQYLYERTYKAGSGLNISHESEQKTIAKTTLP
                     GGYVVRVNGPHDTGTGLYFLGDGITHAVIAGSFLTVGLVADDNRALQTASQLAEGIIA
                     NGIVVQTLKHVTGRENPNTYSDPGGRWRPFPNQKDYAKHVNKYDAFPSGHLSTAMVTV
                     TVISSNYPEYSFVKPVGYTLMGALAFQMVNNGVHWYSDYPLAIAMGWVFGKIAVDRGR
                     VKATDSASAPVCFGPRYVSGRPGAGITIPFGGPRSKRRFL"
     gene            775..>1042
                     /locus_tag="PK362_12140"
     CDS             775..>1042
                     /locus_tag="PK362_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75621.1"
                     /translation="MKRRRWFLPLLLAHFMSASLRAAEDIPDDKKPLPAWVVPAAITG
                     GALAVSFALDWKMHDRLDQYAKGEMGEWVGPKFGKPGGVAETLGR"
BASE COUNT          223 a          312 c          294 g          213 t
ORIGIN      
        1 tctcttggtc gcgggaaccg gcgtgctcat ttacttggat caatatttgt atgaacggac
       61 ttacaaagcc gggagcggcc taaatatctc ccacgagagc gaacaaaaaa ccatcgccaa
      121 aaccactctt cccggaggat atgtggtccg ggtgaacggg ccccatgaca caggaactgg
      181 cctctatttt ctgggcgatg gaatcacaca tgccgtgatc gcgggcagtt ttttaacggt
      241 gggtttggtg gcggacgaca accgcgccct ccaaaccgcc agccaactgg ccgaaggcat
      301 catcgccaac ggcatcgtgg tccaaacctt gaaacacgtg accggccgtg aaaacccaaa
      361 cacctacagc gatccgggcg ggcgctggcg tcctttcccg aaccaaaaag attacgccaa
      421 acacgtgaac aaatacgacg cctttccgtc gggccacctc tccaccgcca tggtcaccgt
      481 gaccgtcatc tcatcgaact acccggaata ctcctttgtc aaacccgtgg gctatacgct
      541 catgggcgcg ttggcgtttc agatggtgaa caacggcgtc cattggtaca gcgattatcc
      601 cctcgccatt gccatggggt gggtttttgg taagatcgct gtggaccggg ggcgagtgaa
      661 agcaacggac tccgcctcag caccggtctg tttcgggccc cgttatgtat cgggtcggcc
      721 cggagcgggg atcacgatcc ccttcggtgg tccgcgatca aaaaggcggt tcttatgaaa
      781 cgacgacgtt ggttcctgcc cctcttattg gcacatttca tgtccgcctc tctccgcgcc
      841 gccgaagata tccccgacga taaaaagccg ctcccggcgt gggtcgtccc cgcggcgatc
      901 acgggcgggg ccttggctgt ctccttcgct ttggattgga aaatgcacga tcggcttgat
      961 caatacgcca agggagaaat gggggaatgg gtcgggccca aattcggcaa accggggggt
     1021 gtcgctgaga ccctcggccg cc
//
LOCUS       DAOQLS010000867         1041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_139677_length_1041_cov_6.600406, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000867 DAOQLS010000000
VERSION     DAOQLS010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139677_length_1041_cov_6.600406"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK362_12145"
     CDS             complement(<1..468)
                     /locus_tag="PK362_12145"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNC75622.1"
                     /translation="MTKYIFVTGGVVSSLGKGIAAASLGRLLKSRGLNVTMIKLDPYL
                     NVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDVDMTRENNCTSGQIYETVIQQE
                     RRGEFLGKTVQVIPHITNEIKNRLRRVTKGRDIVLVEVGGTVGDIESLPFLEAI"
     gene            complement(465..>1041)
                     /locus_tag="PK362_12150"
     CDS             complement(465..>1041)
                     /locus_tag="PK362_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNC75623.1"
                     /translation="GEAVMTSDRCRSGTDRVAEVARRIRADLYLNIQGDEPLMTTRTV
                     RRVLALHRDASVAMGTAATALAKTDWANPNAVKVLTDRRGDALYFSRGALPYYRDGAP
                     AAPPATPLLQKHLGIYSYRADLLRQFVRWPAGYFETAEKLEQLRALEHGVRIRVATTP
                     DDSVGVDTPADAARVERRLRRSRQARGRQAI"
BASE COUNT          171 a          350 c          325 g          195 t
ORIGIN      
        1 gatggcttcc aggaagggca gggactcgat gtcgcccacc gtgccgccca cttcgaccag
       61 gacgatgtcc cggcctttcg tcacccggcg caggcggttt ttgatttcgt tcgtgatgtg
      121 cggaatcacc tggacggtct tcccgagaaa ttccccccgc cgctcctgct gaatcaccgt
      181 ttcgtagatt tggccggagg tgcaattgtt ttcccgggtc atgtccacgt cgatgaaccg
      241 ctcgtagtgg cccagatcca aatcggtctc ggccccgtcc tccgtgacat acacctcgcc
      301 gtgctggtag gggctcatgg tgccggggtc cacgttcaaa taggggtcga gtttgatcat
      361 cgtcacgttc agcccccggg atttaagcaa gcggcccagg gacgcggcgg cgatgccctt
      421 ccccagggag ctgaccaccc cgccggtcac aaaaatatat ttcgtcatat cgcttgcctc
      481 ccgcgcgcct ggcgcgaccg tcgaagacgt cgttccaccc gggcggcgtc ggccggcgtg
      541 tccaccccga cggaatcgtc gggcgtcgtc gccacgcgaa tccgcacccc gtgttccagg
      601 gcccgcaact gctccagctt ttccgccgtt tcgaaatacc cggcgggcca gcggacgaat
      661 tgacgcaaca ggtcggcccg gtaggaataa atgcccaaat gtttttgcag aagcggcgtc
      721 gcgggcggcg cggcgggagc cccgtcgcgg taatacggca aggcgccgcg ggagaaatac
      781 agggcgtcgc cccgccggtc cgtcaacacc ttgacggcgt tggggttggc ccaatcggtc
      841 ttggcgagcg ccgtggccgc cgtccccatg gccacggacg cgtcccgatg gagggccaac
      901 acgcgccgga cggtgcgggt cgtcatcagg ggttcgtccc cttggatgtt caaataaaga
      961 tcggcgcgga ttcggcgagc cacttcggcc acccggtccg tgccgctccg gcaacgatcc
     1021 gaggtcatca ccgcttcccc g
//
LOCUS       DAOQLS010000868         1041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_139727_length_1041_cov_3.456389, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000868 DAOQLS010000000
VERSION     DAOQLS010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139727_length_1041_cov_3.456389"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..>1041
                     /locus_tag="PK362_12155"
     CDS             175..>1041
                     /locus_tag="PK362_12155"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNC75624.1"
                     /translation="MILARYLTGQFLPPFFFGLSIFSGVLLLDKLFDLLDLLINKGVS
                     LWTSAQIFFLFFPTILSLSVPMALLLACLLTFGRLSEDNEILALRASGLSFRQILWPP
                     LAVAGLVSLALLPFNTHLTPTAMDQFRSLYHRIATTDPLIQIEPRRFLNVQNVRLFAG
                     DVSKDRKNLSNVWIYRLFPGYSQRIYADRGTAEITNRQLSLRLEEGQIERFSRGIATD
                     FSHVDFQHYTVNVPFQGALNARDRGWREYTTPGLLKEIHRRRSRRIATSDIEAEMHLR
                     YALAFAPLCLALV"
BASE COUNT          193 a          350 c          256 g          242 t
ORIGIN      
        1 cggtacccgg cggtccgagg aatcgtggtg cgcatgggga tcattctatt tctaagcaac
       61 gttttaacca agacatttca agccctacaa gcataaaccc ctaaacgagc gatttcaatt
      121 gaaaaatcaa cctgttccgg tcgacttcca acgccgaaat tatataatca acgggtgatc
      181 ctggcgcgct atctcactgg ccagttccta ccccctttct tctttgggct gtccatcttt
      241 tccggcgtcc tcttgttgga taagcttttc gatctcctcg atctgctgat caacaagggc
      301 gtttccctat ggacctcggc ccaaattttc ttcctcttct ttccaaccat tctcagtttg
      361 agcgtaccca tggcgctttt gttggcctgc ctgctgacct tcggccggct ctcggaagac
      421 aacgagattc tggccctgcg ggcctcgggc ctgtcctttc gccaaatcct ctggccgcct
      481 ctcgcggtgg ccggactggt gtccctcgcc cttctcccct tcaacaccca cctgaccccg
      541 acggccatgg accaatttcg aagtttgtac caccgcatcg ccacgacgga cccgctgatt
      601 caaatcgaac cccggcggtt cctcaacgtc caaaacgtgc gcctcttcgc gggcgacgtt
      661 tccaaggacc ggaaaaattt gagcaacgtc tggatctacc ggcttttccc gggctattcc
      721 cagcgcattt acgccgaccg gggcacggcg gaaatcacca accgccaatt gtccctccgc
      781 ctggaggaag ggcagatcga gcgcttctcc cggggcatcg cgacggattt ttcccacgtg
      841 gatttccagc actacaccgt caacgttccc ttccaggggg ccctgaacgc ccgggaccgg
      901 ggttggcggg agtacacgac cccggggctg ttgaaagaga tccatcgccg ccggtcccgc
      961 cggatcgcca ccagcgacat tgaggccgaa atgcacctgc gttacgccct ggccttcgcg
     1021 cccctttgcc tggccctggt g
//
LOCUS       DAOQLS010000869         1041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_139738_length_1041_cov_3.295132, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000869 DAOQLS010000000
VERSION     DAOQLS010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139738_length_1041_cov_3.295132"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK362_12160"
     CDS             <1..197
                     /locus_tag="PK362_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75625.1"
                     /translation="DGQPDPESFLDALQAFGQSFRDPRRRAALVRGALAVTPEVAVDR
                     TAREMAAFFGALPARPAVPA"
     gene            complement(194..847)
                     /locus_tag="PK362_12165"
     CDS             complement(194..847)
                     /locus_tag="PK362_12165"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNC75626.1"
                     /translation="MIGPLTQWILDALRTHGGWSVFVGVLIEQIVVPIPSPAIIMGAG
                     FLLIPAGAPWASALGTASLQIVLPGVAASTLGAIGTYAVGRYGGKAFVDRFERFLGFN
                     WKDVESLGAHFSRRGEATSLFLLRAAPIVPLSLISVVSGVLEIPPRLFVLWSVLGTIP
                     RCYLLGVLGWQMGSKALFFAKGVDRFETLFSGLIAAGVVGGILWIRRRVRKGLAEKN"
BASE COUNT          191 a          379 c          318 g          153 t
ORIGIN      
        1 gggacggcca accggacccc gagtctttcc tggacgccct tcaggccttc ggtcaatcct
       61 ttcgggatcc ccggcggcgc gccgcgctgg ttcggggggc cctggccgtc acccccgagg
      121 tcgccgtgga ccgcaccgcc cgggaaatgg cggccttctt cggagccctt cccgcccgtc
      181 ccgcggtccc cgcctagttc ttctccgcca aaccctttcg gacccggcgg cggatccaaa
      241 ggatcccccc caccacgccc gccgcgatca acccggaaaa caaggtttca aatcgatcga
      301 cgcccttcgc gaagaagagc gccttggatc ccatctgcca gcccaacacc cccagcaaat
      361 agcaacgggg aatggtcccc aacaccgacc aaaggacgaa gaggcgcgga gggatctcca
      421 gcacccccga gaccaccgag atcagggaca gcggcacgat gggcgcggcc cgcaacagga
      481 agagggacgt cgcctcgccc cgacggctga aatgggcgcc caggctttcg acgtccttcc
      541 aattgaaccc caggaaccgt tcgaagcggt ccacgaaggc cttccccccg taacgtccga
      601 cggcgtaggt cccgatggcc cccagggtgg aggccgccac ccccggcagg acgatttgaa
      661 gggaggccgt gccgagggcc gacgcccaag gggcgccggc ggggatcagg agaaatcccg
      721 cccccatgat gatggcgggc gagggaatgg gcacgacgat ctgctcgatc aacacgccca
      781 caaaaacgct ccacccgccg tgggtccgaa gggcgtccaa aatccactgc gtgaggggcc
      841 cgatcacgcg ggcccccgcc ggttcgattt ccggggccgt cggccctgat atataatagt
      901 ctccatgtcg cgtcgttcct ccctgttcgc cctcgggatc gccggaatct tcttgacggg
      961 gtgtcccaag gtccccgtca ccggccgtca ctcgctgatt ctgttgccgg aatcggagga
     1021 gttgaccctc ggggtcgacg c
//
LOCUS       DAOQLS010000870         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_140034_length_1040_cov_3.407107, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000870 DAOQLS010000000
VERSION     DAOQLS010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140034_length_1040_cov_3.407107"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PK362_12170"
     CDS             complement(<1..238)
                     /locus_tag="PK362_12170"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNC75627.1"
                     /translation="MTAKPDLIELLSRRPGTRVSGSDLAQALGMTRAAVHKRVAKLRA
                     QGFRIDGASRLGYRWVGAADRLEPGGLRRGFVRDV"
     gene            complement(336..>1040)
                     /gene="nadC"
                     /locus_tag="PK362_12175"
     CDS             complement(336..>1040)
                     /gene="nadC"
                     /locus_tag="PK362_12175"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNC75628.1"
                     /translation="EAFRLMGGARLRFLAKDGDRVRPGRTVLEVRGPLRALLSAERTA
                     LNVLSHLSGVATLTRRFVDGAGRKSLQILDTRKTLPGLRDMEKYAVRCGGGVNHRRDL
                     AAAVLIKENHLSFYRTDRDRRDLARRVAAARRRGRTVEMECRNRAEIRWGLSVGADIL
                     LLDNFSAAALPAVVRWTRAEARRRGAPVPRLEVSGGVTLPVIGRLARSGVDRVSIGRL
                     THSAPALDFNMDVEIQ"
BASE COUNT          170 a          377 c          346 g          147 t
ORIGIN      
        1 ggacatcgcg cacaaaaccg cgccgaagcc cccccggttc caggcggtcc gcggcgccga
       61 cccagcgata gcccagccga cttgcgccgt cgatgcgaaa accttgggcc cgaagtttgg
      121 ccacacgctt gtgcacggcc gcccgggtca ttccgagggc ctgggccagg tcgctgcccg
      181 acacccgcgt tccgggccgc cgggaaagca attcgatcaa atcgggtttc gccgtcaagg
      241 gatggcctcg cccggcccgg ctcggccacc gttcacgccc ggctttgacc cgtgcgaaca
      301 cgggagggtt tttatatccg ggcggtccct ccgcgtcatt ggatttccac gtccatattg
      361 aaatccaggg cgggggccga atgcgtgagg cggccgatgg acacccggtc cacgcccgat
      421 cgggccaggc ggccgatcac gggaagggtc accccccccg acacctccaa ccggggcacc
      481 ggcgctcccc ggcgccgggc ctccgcccgg gtccaccgga ccaccgcggg caaggcggcc
      541 gccgaaaaat tgtcgagcaa caagatgtcg gcgccgaccg acagccccca ccgaatttcc
      601 gcccggttcc ggcactccat ttcgaccgtc cgcccccggc ggcgggccgc ggccacccgc
      661 cgggccaagt cccggcggtc ccggtccgtc cggtaaaaag agagatgatt ttccttgatc
      721 agaacggcgg cggccaaatc ccggcggtgg ttgacgccgc ccccgcaccg gacggcgtat
      781 ttttccatgt cccgaaggcc gggaagggtt ttgcgggtgt cgaggatttg aagggatttc
      841 cgcccggcgc cgtccacgaa ccggcgcgtc agggtggcga cccccgagag atgggacaaa
      901 acgttcaagg ccgtgcgctc cgcgctcagg agcgcgcgca ggggcccgcg gacttcgagc
      961 accgttcggc cgggccggac gcggtcgccg tctttggcca ggaatcgaag gcgggcgccg
     1021 cccatcaagc ggaaggcttc
//
LOCUS       DAOQLS010000871         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_140322_length_1039_cov_3.084350, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000871 DAOQLS010000000
VERSION     DAOQLS010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140322_length_1039_cov_3.084350"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1039)
                     /locus_tag="PK362_12180"
     CDS             complement(<1..>1039)
                     /locus_tag="PK362_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75629.1"
                     /translation="GLGVSLASQVLITRSLGPVNLGNFSFLSNFFSQWIDFFDAGASL
                     CFYNRLSQRRESALIRFNWEFLSWIGATVALGIGAVAALGWGGKIWPDQHWSWVWWAF
                     LLAFLLKIVQTAGQIIDAFGHTVEGERARTGQRILALLLLAGLCGGRAIGLPTFFGYN
                     TTIALGLLGAWYVVLRKKKAPLFPAEPLTAEARREYARAYWTYSHPLVVYCLVGFAAS
                     VLDRWLLQTYAGSVDQGFFGFSFQWSSVCFLFSGAMVPLIMREFALAFQRNDAEGIRW
                     RFERYVPLLYSITGFLAAFVAVEARTLAVLAGGERFLNAAGPLAVMAYFPLHQMLGQV
                     CASYFYATGRTT"
BASE COUNT          243 a          338 c          325 g          133 t
ORIGIN      
        1 cgtggtgcgc cccgtggcgt agaaatagga cgcgcacacc tggccgagca tctggtgaag
       61 cggaaaatag gccatcaccg ccagggggcc cgccgcgttc agaaaccgct cgcccccggc
      121 caacaccgcg agggtccggg cctccaccgc cacgaaagcg gcgaggaatc cggtgatgga
      181 ataaagcagc ggcacgtagc gttcaaagcg ccagcggatg ccctcggcgt cgttccgttg
      241 gaaagccagg gcgaactccc gcatgatgag gggcaccatg gccccggaaa acaggaagca
      301 cacggaactc cactggaagg aaaacccgaa aaatccctgg tcgacggacc cggcgtaggt
      361 ctgaagcagc caacggtcca ggacgctggc ggcgaaaccc accagacaat aaaccacgag
      421 ggggtgggag taggtccaat aggcccgggc gtactcgcgc cgcgcctcgg cggtcaaggg
      481 ctccgcggga aagagcggcg ctttcttttt tcgaaggacg acataccacg cccccagaag
      541 ccccagggca atcgtcgtgt tgtagccgaa aaacgtcggc aggccgatgg cccgcccgcc
      601 gcagagcccc gccagcaaaa gcagcgccag gatccgctgc ccggtccggg cccgttctcc
      661 ctcgaccgtg tggccgaagg cgtcgatgat ctggcccgcc gtctggacga tcttcagcaa
      721 aaaagccagg aggaaagccc accacaccca ggaccagtgt tggtccggcc atatttttcc
      781 gccccatccc agggccgcga cggcgccgat cccgagggcc acggtggcgc cgatccagga
      841 caaaaattcc cagttgaatc gaatcagcgc gctttcccgg cgctgggaca accgattgta
      901 aaaacacagg gaggccccgg cgtcgaaaaa atcgatccac tggctgaaaa aattggagag
      961 gaaactgaag ttgccaagat tgaccgggcc cagggaccgg gtgatcagga cctggctcgc
     1021 cagggacacc cccagcccg
//
LOCUS       DAOQLS010000872         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_140433_length_1039_cov_2.156504, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000872 DAOQLS010000000
VERSION     DAOQLS010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140433_length_1039_cov_2.156504"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PK362_12185"
     CDS             <1..586
                     /locus_tag="PK362_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC75630.1"
                     /translation="GPSGSGKSTLLNILGGLDSPTAGSVFFGDHDLVAASAQDLTLFR
                     REHVGFVFQFYNLIPNLTAEENVALVTALAKNPMTPAEALERVGLGERRRHFPAQMSG
                     GEQQRVAIARAVAKRPDILLCDEPTGALDQTTGKQVIQALLDVNRDTGTLAVVITHNA
                     AIAGVADRVVRLADGRIASDQKNAARRSLEDIEW"
     gene            580..>1039
                     /locus_tag="PK362_12190"
     CDS             580..>1039
                     /locus_tag="PK362_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC75631.1"
                     /translation="MVTGLLRKLPRDLWTLRAQTAALAALVLCGVAVLVSSWSSYRSL
                     QAALFDYYNRYRMAELFAEVKRAPNPLLDRLRALPGVDTAETRLAEDVLLDVPGQPEP
                     ALGHFVSLPADGEPLLNRIYLRGGRAPRAGDPPEVLVHEAFAQAHRLKAGD"
BASE COUNT          164 a          384 c          330 g          161 t
ORIGIN      
        1 gggcccctcg ggcagcggga aatccacctt gctgaacatt ctgggcggcc tcgacagccc
       61 caccgccggg tcggtctttt tcggcgacca cgacctggtg gccgcgtccg cccaggacct
      121 cacgctcttt cggcgcgagc acgtgggttt tgttttccag ttttacaacc tgatccccaa
      181 cctgaccgcc gaggaaaatg tggccctggt gacggccttg gccaaaaacc ccatgacccc
      241 cgccgaagcg ctggaacggg tcggcttggg ggagcgccgc cggcactttc cggcgcagat
      301 gtcggggggc gaacagcaac gggtcgccat cgcccgggcc gtggcgaagc ggccggacat
      361 tttgctgtgc gacgaaccca ccggcgccct ggaccaaacc acgggcaaac aggtcatcca
      421 agcgcttctc gacgtcaacc gggacaccgg caccctggcc gtcgtcatca cccacaacgc
      481 cgccattgcc ggggtggccg accgcgtggt gcggctggcc gacggacgca tcgcctcgga
      541 ccaaaaaaac gccgcgcgac gctccctgga ggacatcgaa tggtgacggg cctccttcgc
      601 aaactgcccc gggacctttg gacgctccgg gcccagaccg cggccctggc ggccctggtc
      661 ctctgcgggg tggcggtgct cgtgtcctcc tggagctcgt atcggtctct ccaggccgcg
      721 ctcttcgact attacaaccg ctaccggatg gccgaacttt tcgcggaggt gaaacgggcc
      781 cccaaccccc tgctggaccg cttgcgggcc ctccccggag tggacacggc cgaaacgcgc
      841 ctcgcggagg acgttctgtt ggacgtgccc ggccagcccg agccggccct ggggcacttc
      901 gtgtccctcc ccgccgacgg cgaacccctg ctgaaccgga tttacctgcg cggcgggcgc
      961 gccccccggg ccggcgaccc gcccgaggtc ctggtgcacg aagccttcgc ccaagcccat
     1021 cgcttaaaag cgggagacc
//
LOCUS       DAOQLS010000873         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_140657_length_1038_cov_2.789420, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000873 DAOQLS010000000
VERSION     DAOQLS010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140657_length_1038_cov_2.789420"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..720)
                     /locus_tag="PK362_12195"
     CDS             complement(31..720)
                     /locus_tag="PK362_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC75632.1"
                     /translation="MSAAPAVSVRGLGKRFGTVDVLTDLSFDVQPGERVCVWGPSGAG
                     KSTLLHVLGLMTPPSTGALTLFGRDAAGLSMAERARWRNEKIGFLFQFHHLLPDLSLL
                     ENVMIPLLIRRRSPDEAAARSRDLLVRLGLGHRLEHRPADASGGERQRAALARALVGE
                     PALLLADEPTGNLDRGIGREVEALMNDEARARGATLILVTHDETLAAHMDRRLGLVDG
                     RLAEPVPGRAR"
     gene            complement(717..>1038)
                     /locus_tag="PK362_12200"
     CDS             complement(717..>1038)
                     /locus_tag="PK362_12200"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC75633.1"
                     /translation="REIGILGAMGATPGMIQRIFLLKGLLMGAFGTAAGTVLGLAISG
                     LLKKYEFVKLPADVYYVERLPVRVVPSDVLLVIVSALLIVLIATLYPSRAAAKMDTLE
                     AVRQ"
BASE COUNT          191 a          364 c          332 g          151 t
ORIGIN      
        1 ggttgggttg gagcggtcac gttttctcct tcaccgggcg cggcccggaa ccggttcggc
       61 caagcggccg tccacgagcc ccagccgccg gtccatgtgc gcggccagcg tttcgtcgtg
      121 cgtcaccaaa atcagcgtgg cgccccgggc gcgcgcttcg tcgttcatca gggcttccac
      181 ctcgcggccg atcccgcggt ccaaattgcc ggtcggttcg tcggccaaca acagggccgg
      241 ttcgcccacc aaggcccggg ccagggcggc gcgttgccgc tctccccccg aggcgtccgc
      301 cggacgatgt tccagccggt gcccgaggcc caaccggacc aacaggtccc gggaacgcgc
      361 ggccgcctcg tcgggcgacc gccgtcggat gagaagggga atcataacat tttcgaggag
      421 ggacaaatcc gggagcaggt ggtggaattg aaacagaaaa ccgatctttt cgttccgcca
      481 gcgggcccgc tccgccatgg acaagccggc cgcgtcccgg ccaaagagcg ttaaggcgcc
      541 ggtcgagggc ggcgtcatga gtcccagcac gtgcaacagc gtggatttcc cggcccccga
      601 cggcccccac acgcagaccc gctcgcccgg ttggacgtcg aaggacaggt ccgtcaggac
      661 atcgaccgtc ccgaaacgct tgcccagccc ccggaccgag accgccggcg cggcgctcat
      721 tggcggacgg cctccagggt gtccattttg gcggccgccc gggaggggta gagggtggca
      781 atcaacacga tgagcagggc gctgacgatc accaacaaca catcggacgg gaccacacgg
      841 acgggcaacc gttccacgta atagacgtcg gccggaagtt tgacgaactc gtatttcttg
      901 agaagccccg aaatggcgag ccccaggacc gtccccgccg ccgtgccaaa agcccccatc
      961 aggagccctt tcaggaggaa aatgcgctgg atcatccccg gcgtcgctcc catggccccc
     1021 aaaataccga tttcccgg
//
LOCUS       DAOQLS010000874         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_140887_length_1037_cov_3.112016, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000874 DAOQLS010000000
VERSION     DAOQLS010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140887_length_1037_cov_3.112016"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PK362_12205"
     CDS             complement(<1..827)
                     /locus_tag="PK362_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75634.1"
                     /translation="MEKGRLASVDGQARIIGALLVQNGTQYVMDKEGNVIALSELRES
                     IGPMTERVKSLVAQTEQIQKGLANAYDRLNDLETLRARYGDRGLLKETFHIAADRSAA
                     WRMSSLRTEAATLADTSDLLSQLVDEGSADIAFSLFRESTRTEKALSQSLAGLASGLA
                     GFEEATTSLKTNFEILNRIAANATEYNLTRLDDSKILTKAAEAFYSARQTILKSPLTT
                     RVVARELGKTGEGFKALLSRGIDRQWADRESRNAINTAPPVKVRPTPALDWLIHPVRT
                     "
BASE COUNT          196 a          298 c          306 g          237 t
ORIGIN      
        1 gtccggaccg ggtgtatcaa ccaatccagg gcgggggtgg ggcgcacttt gacgggaggg
       61 gccgtgttga tggcattccg cgactcccga tccgcccatt gccggtcaat cccgcggcta
      121 agcaatgcct tgaatccttc cccggttttg ccaagttcac gcgccacaac gcgtgtcgtt
      181 aaagggcttt tgaggatggt ttgtcgggcc gaataaaacg cctccgccgc cttggttaga
      241 atctttgaat catccaggcg ggtcaggttg tattccgtcg cgttggcggc aatgcgattt
      301 aatatttcaa agttggtctt aaggcttgtg gttgcctcct caaacccggc caagccgctc
      361 gcgagccctg ccaaagattg cgacaaggcc ttttccgttc gggtcgattc ccggaagagc
      421 gaaaaagcga tgtccgcgga gccttcgtcg accagttgcg aaagaaggtc ggaggtgtcc
      481 gccaacgttg cggcctcggt ccgcagggag ctcatgcgcc aagccgcgct tcggtccgcc
      541 gcgatgtgaa acgtctcttt caataggcca cgatcgccgt atcgggcccg gagggtctcc
      601 aaatcgttca atcggtcgta ggcgttcgcg agaccctttt ggatttgttc cgtttgggcc
      661 acaagggatt tgacgcgttc cgtcattggg ccgatcgatt ccctcagttc gctcagggcg
      721 atgacgttgc cttccttgtc cattacgtat tgagtgccgt tctgaaccaa cagcgcgccg
      781 atgatccgcg cttgaccgtc caccgaggcc agccgccctt tttccaccgt gtaggtttgg
      841 cccgagatcg atatttgacc cctcgtgagg gtccactgtt cgttgtttgg atcatccgaa
      901 gagagaaaag gcttatcaag accgtcaaca acgaaggaga cattgggaag gcccaggctt
      961 cccgacggaa gcgcaaacgc ttggcttccg cccgcctcga tggtgaatac gccgggtttg
     1021 acgagagcga gagcccc
//
LOCUS       DAOQLS010000875         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_141370_length_1035_cov_4.801020, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000875 DAOQLS010000000
VERSION     DAOQLS010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141370_length_1035_cov_4.801020"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK362_12210"
     CDS             complement(<1..312)
                     /locus_tag="PK362_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75635.1"
                     /translation="MIALFWPRQREERELWVASATILFQELLFIRWIGTEVRVLAYFP
                     NLLLLAAFLGLGLGCLAVNGKPWAPFWPWLLAAVTLATAGLSRVVFTAEGASEHLWLL
                     YY"
     gene            391..1029
                     /locus_tag="PK362_12215"
     CDS             391..1029
                     /locus_tag="PK362_12215"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNC75636.1"
                     /translation="MGQLVLVRHGQSQWNLENRFTGWVDVPLTDQGRNEARRAGEHLR
                     GYKFNRAFTSALQRAQNTLAIILEVTGQKTIPIEKNQALNERHYGALQGLNKEETAKK
                     FGADQVKVWRRSYDVPPPTDKTDMNPEGIAESLKDTAARTLPYFYSAILPHVKKGESV
                     IVAAHGNSLRSIVMDLDRLTKEQVLELNLDTGVPLVYDIDEKGRVTGKKILK"
BASE COUNT          261 a          298 c          319 g          157 t
ORIGIN      
        1 gtagtaaagc agccacaaat gttcggacgc tccctcggcg gtaaagacga cccgggacaa
       61 ccccgccgtg gcgagggtga ccgccgcgag cagccacggc caaaacgggg cccagggttt
      121 accgttcacg gccaggcatc ccaggcccag ccccaaaaaa gccgccagaa gcagaaggtt
      181 ggggaaataa gccagaaccc ggacttcggt gccgatccat cgaatgaaaa ggagttcctg
      241 gaaaaggatc gtcgccgagg cgacccagag ttcccgttct tcgcgttggc gcggccaaaa
      301 gagggcgatc attagattgc tataataccg aattcaaagc gcgctccgcc ggggtagccg
      361 gcggctattt taccgttgga ggccgaagtc atggggcaat tggtgttggt gcgtcacggg
      421 cagtcgcagt ggaatttgga aaaccgtttc accggttggg tggacgttcc gttgacggat
      481 caagggcgaa acgaagcccg ccgcgcgggc gaacaccttc gggggtataa attcaaccgg
      541 gccttcacct cggcccttca gcgggcccaa aacaccctgg cgatcatttt ggaagtcacc
      601 ggacaaaaaa ccatcccgat cgagaagaac caggccctga acgagcgcca ctacggggcc
      661 ctgcaggggt tgaacaagga ggaaacggcg aagaaattcg gcgccgatca ggtcaaggtg
      721 tggcggcgca gctacgacgt gcccccgccg acggataaaa ccgacatgaa cccggaaggc
      781 atcgccgaaa gcctgaagga cacggccgcc cggaccctcc cctatttcta cagcgccatt
      841 ctcccccacg tgaaaaaagg cgaaagcgtc atcgtggccg cccacggcaa cagcctgcgg
      901 tccatcgtca tggatttgga caggctcacg aaagaacagg tgttggaact caatctcgac
      961 acgggcgtgc cgctggtgta cgacatcgac gaaaaagggc gggtgacggg gaagaaaatc
     1021 cttaaataag ggggc
//
LOCUS       DAOQLS010000876         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_141674_length_1034_cov_3.721144, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000876 DAOQLS010000000
VERSION     DAOQLS010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141674_length_1034_cov_3.721144"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1034
                     /locus_tag="PK362_12220"
     CDS             <1..>1034
                     /locus_tag="PK362_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC75637.1"
                     /translation="EANPPLEWSETKNVKWKVAIPGEGSSTPAIWGDRVFILTAFSPT
                     KKADGKPTAEARPGGPPPEGRPDGPPPGPGGRKRGPGGPGGPGGPGGFGGGEKPTEAY
                     QFMVLCLDRQTGKTLWQKVAREEVPADARHRNNTYASGSPITDGQVVVAYFGSAGLFC
                     YDVDGKLKWEKQLGRMQTRNSFGEGASPALFGDTLIVNWDDEQDRDFIAAFDKNTGKE
                     LWRTPRSDATTWSTPLVVDQSGKRQVIVNATSKVRSYDLASGKEIWSCAGQTANAIPS
                     PVADAETVYVTSGFRGSALFAIKLGATGDVAGTDAVRWSHDRDTPYVPSPLLVDGLLY
                     FAKGNNGVLS"
BASE COUNT          213 a          314 c          336 g          171 t
ORIGIN      
        1 cggaagccaa tcccccgctg gaatggagcg aaaccaaaaa tgtgaaatgg aaggtggcca
       61 ttcccggaga aggctcgtcc acaccagcga tttggggcga ccgggtgttt atcttgacgg
      121 ctttcagtcc caccaaaaag gccgatggca aaccgacggc ggaagcccgg ccgggcggac
      181 cgccgccaga aggaagaccc gatggcccac cgccgggtcc ggggggacga aagcgcggcc
      241 ctggcggacc cggtgggccc ggcgggccgg ggggattcgg cggcggcgaa aagccgacgg
      301 aggcttacca attcatggtg ctgtgcttgg atcgccagac cggcaaaacc ctctggcaaa
      361 aagtcgcgcg cgaggaggtg ccagccgacg cgcgccaccg caacaacacc tacgcttccg
      421 gttcgcccat caccgatggc caggtggtcg ttgcctattt cggttcggcc ggcctgtttt
      481 gctatgacgt tgacgggaag ctgaagtggg aaaagcagtt ggggcgcatg cagacgcgca
      541 acagctttgg cgaaggcgcg tcgccagcct tgttcggcga taccctcatc gtgaactggg
      601 acgatgagca ggaccgcgac ttcatcgcgg ccttcgataa gaacaccggc aaggaactct
      661 ggcggacacc ccgcagcgat gccacaacgt ggtccacgcc cctggttgtg gaccaaagcg
      721 gcaaacgaca agtgatcgtc aatgccacca gcaaggtgcg cagctacgat ctggcatccg
      781 gtaaagaaat ttggtcctgt gccggccaga cggccaacgc cattcccagt ccggtggcgg
      841 atgcggaaac tgtttacgtc acgagcgggt ttcggggttc ggcgttgttc gccatcaaac
      901 tcggtgccac tggcgacgtg gcgggaacgg acgcggtccg ttggagtcat gatcgcgaca
      961 caccctacgt cccctccccg ctgctggtgg atggcctgct ttatttcgcc aagggtaaca
     1021 acggcgtcct ctcc
//
LOCUS       DAOQLS010000877         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_141740_length_1034_cov_2.748723, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000877 DAOQLS010000000
VERSION     DAOQLS010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141740_length_1034_cov_2.748723"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PK362_12225"
     CDS             complement(<1..375)
                     /locus_tag="PK362_12225"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNC75638.1"
                     /translation="MREGGVPGRWRAADHCFRRDPYWTAALLLAAVFAAVTIAFKLIQ
                     FNSLQTQLYDLGETSNVLWNTFHGRPFWTSVMEENYLGQHFAPALALLGPLVVVTGSA
                     GSLIVVQCLVFAAATVGVFVFAR"
     gene            complement(368..>1034)
                     /locus_tag="PK362_12230"
     CDS             complement(368..>1034)
                     /locus_tag="PK362_12230"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC75639.1"
                     /translation="SADTVVWTVNAGLSDVAQGGVRAVWRGAGFIEETLRGKTFRISP
                     FTFFQTNTRGAEELYGVVADFVGGRVTTLFDLYCGTGSIGLTVADRADRLVGVELNAA
                     AVEDARENARRQGAAHAEFHALDAAAFARSPEFQTLWTAPGAVAVVDPPRPGLQPDVR
                     RLLAEKPVHRWVYVSCNPEALVRDLTALSLVYRTVIVQPVDLFPHTPHVETVVLLERR
                     PGA"
BASE COUNT          215 a          330 c          349 g          140 t
ORIGIN      
        1 ccgagcgaaa acgaacaccc ccaccgtggc ggcggcgaaa accaaacact ggaccacgat
       61 caaggacccg gccgaacccg tgaccacgac cagggggccc aggagcgcca gcgccggagc
      121 gaagtgctgg cccagataat tttcctccat gacgctggtc caaaagggcc gaccgtgaaa
      181 ggtgttccac aaaacgttgg aggtttcccc caaatcgtag agctgggttt gaagggaatt
      241 gaattggatc aatttgaagg cgatcgtcac ggccgcgaag acggcggcca ggaggagggc
      301 cgcggtccaa tagggatcgc ggcggaaaca gtggtcggcg gcccgccaac gcccgggaac
      361 gcccccttca cgcaccgggc cggcgctcca ggagaacgac cgtttcgacg tggggcgtgt
      421 ggggaaaaag gtccaccggt tgaacgatca ccgtacggta aaccaggctc aaggcggtca
      481 gatcccggac cagggcctcg gggttgcagg acacgtagac ccagcggtgg acgggttttt
      541 cggccagaag ccgacgcacg tccggttgaa gtccggggcg gggcgggtcc acgacggcca
      601 cggcgcccgg agcggtccag agcgtctgga attcggggga ccgggcgaag gcggccgcgt
      661 ccagggcgtg gaattcggcg tgggcggcgc cctggcggcg ggcgttttcc cgggcgtcct
      721 cgaccgcggc ggcgttcagc tcgacgccca ccagtcggtc ggcccgatcg gccacggtca
      781 acccgatgga ccccgtgccg cagtataggt caaagagcgt cgtcacgcgg ccgcccacaa
      841 aatcggcgac gaccccgtac aattcctcgg ccccgcgggt gttggtttga aaaaacgtga
      901 aaggcgaaat tcgaaaagtt ttcccgcgca acgtctcttc gataaaaccc gccccgcgcc
      961 agacggcgcg taccccgccc tgggccacgt ccgacaaacc ggcgttaacg gtccaaacga
     1021 ccgtgtcggc cgac
//
LOCUS       DAOQLS010000878         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_142033_length_1033_cov_2.758691, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000878 DAOQLS010000000
VERSION     DAOQLS010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142033_length_1033_cov_2.758691"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK362_12235"
     CDS             <1..399
                     /locus_tag="PK362_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC75640.1"
                     /translation="NQYLWDEEIIARKMVFLSGPRQVGKTTYARGLIGDRFSGSYHNW
                     DNPVVRRSYASDPFFFLPRTSEAKPSLVIFDEIHKRPRWKDILKGIFASLDPAIRLLV
                     TGSARLEWFRKSGDSLVGRYSHFHMMPVSL"
     gene            608..847
                     /locus_tag="PK362_12240"
     CDS             608..847
                     /locus_tag="PK362_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75641.1"
                     /translation="MILTEDLRDITNIKTIETVERLMDLLPERIGSPLSVNSLVPHLE
                     TTFPTIKNYLTQLEKLWVLFSIRPWSQRISRSFRK"
BASE COUNT          256 a          266 c          248 g          263 t
ORIGIN      
        1 aatcaatatt tatgggatga ggaaattatt gcccgcaaaa tggttttcct gtcaggcccc
       61 cgccaggtcg ggaaaacgac ctacgctcgc ggattgattg gagaccggtt ttcaggcagt
      121 tatcacaatt gggataatcc cgtcgttcgg cggagttatg cgtccgatcc tttcttcttt
      181 ctcccgcgca catctgaagc caaaccctcc ctcgtcattt ttgacgaaat ccacaaacgc
      241 ccacgatgga aagatatcct taaaggaatt tttgcctcgc tggatccggc gattcgcctt
      301 cttgtgacgg gaagcgcgcg gctggaatgg tttcgaaaat cgggcgactc gcttgtgggg
      361 cgttattccc attttcacat gatgcctgtc tctctctgaa ttgctgggaa ccgatcccca
      421 tcactggtgg ctgtccgccg aaagagaatg ggaaaacccg tggcgttttc ttatagaacg
      481 tctgacgggt gaccggtcag aatcggtctc cctataccaa acgctctttc aattcagcgg
      541 ttttccggag cctctggcga gaggatctaa acgattcttg cggaaatgga aatcagatta
      601 cctttctttg attttaacgg aagatttaag ggatatcacc aacatcaaaa ccatcgaaac
      661 agtggaacgt ctgatggacc ttctccccga acggattggg agtccgttgt ccgtgaacag
      721 cctggttccc catttggaaa ccacctttcc aacgatcaaa aattatctca cgcaattgga
      781 aaagctatgg gtcctctttt cgattcgccc ctggagccaa cggatttcac ggtcgtttcg
      841 gaaatagaga aaatactatt tcgcgaactg ggtctacgct tcagacgaag ccaaccaatt
      901 cgaaaacatg atcgccagcg ccctcttaaa aaactgcctc acatgggagg accgaggtct
      961 cgggaaaacg gatctctttt acgtaaggac gcacgaaggc caagaggtcg attttctcct
     1021 ctccctggaa ggg
//
LOCUS       DAOQLS010000879         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_142366_length_1032_cov_2.485159, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000879 DAOQLS010000000
VERSION     DAOQLS010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142366_length_1032_cov_2.485159"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1032
                     /locus_tag="PK362_12245"
     CDS             71..>1032
                     /locus_tag="PK362_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNC75642.1"
                     /translation="MTAASPDSPLEAGDARLIPAYTFEEFVVGPHNRFPHAASLAVAE
                     NLGKAYNPLFLYGPVGIGKTHLMQSVGHRVLQRDPKTRVLYVTAQQYMTEVIELLQAG
                     KLQSLRERYRALDLLLVDDIQFLATSEATQEEFFHMFNDLHAGGKQIIMTSDRPPKML
                     TTLEDRLRSRFEWGLIADIKFTNLETRVAILKKKEGHLKGLRLADDIRLYIASRLKSN
                     IRELEGFLRRIQAYTQLYNEEISLDLVKEILKELLPPEEGGGIDAESGESPAADRVRG
                     SAKSARAEIEGFDIQPSQFEPPPPAKPAAKEEPKQTAPSPAEPKP"
BASE COUNT          235 a          337 c          274 g          186 t
ORIGIN      
        1 ggtccaagct cctctacatg gatctcatgg tggaactcat gatgttcaaa cgccggatca
       61 ggcccgtccc atgaccgccg cttccccgga ttcgccgctg gaagccggcg acgcccggct
      121 cattccggcc tacacgttcg aagagttcgt ggtgggcccc cacaaccggt ttccccacgc
      181 ggcgtccttg gccgtggcgg aaaacctcgg gaaagcctac aaccccctgt tcctctacgg
      241 ccccgtgggc atcggcaaaa cccacctgat gcagtcggtg ggacaccggg tccttcaacg
      301 ggacccgaaa acccgcgtgc tttatgtcac cgctcagcaa tacatgacag aggtcatcga
      361 gctcctccaa gccggcaaac tccaatcgct tcgcgagcgc taccgggccc tcgatcttct
      421 cttggtggac gacatccaat ttctggccac ctcggaggcc acccaagaag aatttttcca
      481 catgttcaac gatttgcacg ccggcggaaa gcagatcatc atgacctccg accggccgcc
      541 caaaatgctg accaccctgg aagatcggtt gcgaagccgg ttcgaatggg gccttattgc
      601 cgacatcaaa ttcacgaatc ttgaaacccg ggtggcgatt ctcaaaaaaa aggagggcca
      661 cctgaaaggc ctccggttgg cggacgatat ccgcctctac atcgccagcc gactgaaatc
      721 caacattcgg gaattggagg gatttcttcg gcgtatccag gcctacaccc aactttacaa
      781 cgaggaaatt tcactggacc tggtcaaaga aattttaaag gaactgttgc ctcccgaaga
      841 aggggggggg attgacgccg agtccggaga atcgcccgcc gctgatcgcg tgcgcgggtc
      901 ggccaaatcc gcccgtgctg agatcgaagg attcgacatc caaccgtcgc agttcgagcc
      961 gccccctccg gcgaaacccg cggcgaagga ggagcccaaa caaacggccc cttcgcccgc
     1021 cgaaccgaaa cc
//
LOCUS       DAOQLS010000880         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_142408_length_1032_cov_2.192426, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000880 DAOQLS010000000
VERSION     DAOQLS010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142408_length_1032_cov_2.192426"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..437
                     /locus_tag="PK362_12250"
     CDS             60..437
                     /locus_tag="PK362_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75643.1"
                     /translation="MRTRGVWWGVAGLVLVGMKKVDPVDRLVAELSQKPLWSNGTFPI
                     VPLGAKAAPAEVLRKSFESTYLAEGQIKKFKILEIRKVRVGKLTPENYRAALLETDYG
                     RKIVLFRFLGAESGWWTRIFDAR"
     gene            511..>1032
                     /locus_tag="PK362_12255"
     CDS             511..>1032
                     /locus_tag="PK362_12255"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNC75644.1"
                     /translation="MKRLTNRPKAHAWGPMLLALWGIGCASGPRELPVLGTVGDFRLT
                     AVTGEKTSALSAGALSGQVWIADFIWTRCKGPCFLMSGNMARLQRALPGEIRLVSISV
                     DPERDDPKSLRAYSKHFVGQENRWFFLTGEKAKVYALIENGFKVSAAEDRDAPLAERF
                     VHSTKFVLVDGAGM"
BASE COUNT          223 a          237 c          336 g          236 t
ORIGIN      
        1 atggttaaaa tcgaaaagcc tcgcggtgct tgcatttggt tgagaggcgg gagggtcgtt
       61 tgcggacacg cggagtttgg tggggggtcg ccggtctggt gttggtgggc atgaaaaaag
      121 tggatccggt cgatcgcctg gtggccgagc tctcccagaa gccgttgtgg agcaacggta
      181 ccttcccgat tgtgcctttg ggcgcgaaag cggctccggc ggaggtgctc cgcaaaagct
      241 ttgaatccac ctatttggcc gaggggcaaa tcaaaaaatt taagatcctt gaaatacgaa
      301 aagtgcgcgt tggaaaattg actcccgaga attaccgagc ggcccttttg gaaactgatt
      361 acggacgcaa aatcgttttg ttccggtttc tgggcgccga aagcggatgg tggacacgga
      421 ttttcgatgc ccgataaagg aaatggaaat catgatgcag atcatagatt tggtttggac
      481 gcaattgata aataaagcgc ggttaaaggc atgaaacgat tgacgaaccg ccccaaggcc
      541 catgcgtggg ggccgatgtt gctggctctc tgggggatcg gatgcgcctc cggaccgcgg
      601 gagttgccgg tgttggggac ggtcggtgat tttcggctga ccgccgtgac cggcgagaaa
      661 acatccgctc tgtcggccgg ggccttgtcg ggacaggttt ggatcgccga tttcatttgg
      721 acccggtgca aggggccatg ttttttaatg tccggcaata tggcgcggtt gcaacgagcg
      781 ctgcccgggg aaatccgtct ggtatcgatc agcgtcgatc cggagcggga cgatcccaaa
      841 tcgttgcggg cctattccaa gcattttgtg ggtcaggaaa accgatggtt ttttttgacg
      901 ggcgagaaag cgaaagttta cgccctgatc gaaaacggtt ttaaagtcag cgccgccgaa
      961 gaccgcgacg cccccctcgc cgaacggttt gttcattcaa cgaagtttgt ccttgtggac
     1021 ggggcgggaa tg
//
LOCUS       DAOQLS010000881         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_143384_length_1028_cov_4.727646, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000881 DAOQLS010000000
VERSION     DAOQLS010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143384_length_1028_cov_4.727646"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PK362_12260"
     CDS             complement(<1..710)
                     /locus_tag="PK362_12260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237076.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNC75645.1"
                     /translation="MSSGKVVQVIGPVVDMEFPAGQLPAIHNAVKIDVEAAGGETRVL
                     TAEVAQHLGDNTVRAVTLGPTDGLRRGMAAVDSGAPILVPVGPACLGRLMDVLGEPKD
                     YRGPIEAKEKFPIHRPTPDFTDQETTPQIFETGIKVIDLLEPYMKGGKVGLFGGAGVG
                     KTVVIMELINNVAKQHGGVSVFGGVGERTREGNDLWLEMQQSKLSGGESVLSKTVLVY
                     GQMNEPPGSRLRVALTALT"
     gene            complement(726..>1028)
                     /locus_tag="PK362_12265"
     CDS             complement(726..>1028)
                     /locus_tag="PK362_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75646.1"
                     /translation="VDFFQGTKSSRDNLNWLELSLAGGATVPLVEHVDLFGGVSFVRP
                     DMRFEISNAVTKFDTEQRFGGFGGIGYSPNDAWTLTASVELGAEAVVGMSARYHFQ"
BASE COUNT          189 a          336 c          313 g          190 t
ORIGIN      
        1 gttaaggccg tcaaggcgac ccgcaggcgg gacccggggg gttcgttcat ctggccgtac
       61 accagcacgg ttttggacaa cacgctttcc ccgccggaaa gcttggactg ctgcatttcc
      121 agccaaagat cgttcccctc gcgggttcgt tcaccgacgc cgccgaaaac cgaaacgccg
      181 ccgtgctgct tggccacgtt gttgatgagc tccatgatga cgacggtttt tcccacgccg
      241 gcgccgccga agaggcccac cttgccgccc ttcatgtagg gctcgagcaa gtcgatgacc
      301 ttgatgcccg tttcgaaaat ctggggggtg gtttcctgat ccgtgaagtc cggggtgggg
      361 cggtgaatgg gaaacttttc cttggcctcg atggggcccc ggtaatcctt gggttcccca
      421 aggacgtcca tcaggcgacc caaacaggcc gggcccacgg gaaccaaaat gggagccccc
      481 gaatcgacgg cggccatgcc gcggcgcagg ccgtcggtgg ggcccagggt gacggcgcgc
      541 acggtgttgt cgcccaggtg ttgggccact tcggcggtca acacccgggt ttcccccccc
      601 gccgcctcca cgtcgatttt aacggcgttg tgaatcgccg gcagctggcc cgcggggaac
      661 tccatgtcca ccacgggacc gatgacttga acgacttttc ccgaactcat gaggcgctcc
      721 ttggcttatt gaaaatgata ccgggccgac atcccgacga cggcctcggc gcccagctcc
      781 accgaggcgg tcaaggtcca agcgtcgttg ggcgagtagc cgatgccgcc gaacccgccg
      841 aatcgttgct cggtgtcgaa tttcgtaacg gcgtttgaaa tctcgaaccg catatccgga
      901 cgcacaaaac tgaccccgcc gaacaagtcc acgtgctcga ccaggggcac cgtcgcgccc
      961 ccggccaggg agagctccag ccagttcaag ttgtcccggc tggatttggt cccttgaaaa
     1021 aagtccac
//
LOCUS       DAOQLS010000882         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_143661_length_1027_cov_5.407407, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000882 DAOQLS010000000
VERSION     DAOQLS010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143661_length_1027_cov_5.407407"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..877)
                     /locus_tag="PK362_12270"
     CDS             complement(161..877)
                     /locus_tag="PK362_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75647.1"
                     /translation="MKSSTALIVITLSLAAAGCRRDDIAYYTVPKESDAPNLSVPAAA
                     PSSMPSPGTPAETAEEPKSSLPPGHPDISGAPGMDLGGAQAAGIAPSAGPENRVTWKT
                     PKGWIEKPGSGFRYATFVVPGPDGRTGDLSVTVLDGDAGGLLSNINRWRDQIGLSALA
                     EGDLGAQTERATLGGRPMTLVNFVSAEPMIDGKFKKRLRAALFAADGRTWFFKLTGDD
                     ALVAGADRSFRAFLASLKGL"
BASE COUNT          214 a          302 c          328 g          183 t
ORIGIN      
        1 atatggacct ggtccagggt tccgaaaacc acgagaagaa tcatcatggc catgagcgta
       61 atggtcaatt ccagggaccc cagaacccgt tggacggcgg gaaggggcga agcgtcgggg
      121 ggcgcgggcg gaagattcgg ggaagccttc ttgttttttt tcatagcccc ttcaaactgg
      181 ccagaaaggc ccggaacgac cggtcggccc cggccaccag agcatcgtcg cccgtcaatt
      241 taaaaaacca cgtgcggccg tccgcggcga acagagcggc ccgaagtcgc ttcttgaatt
      301 ttccatcgat catgggctcg gcgctcacga aattgaccaa ggtcatgggc cgcccgccca
      361 gggtcgcgcg ctccgtttga gcgcccaggt cgccctccgc caaggcggaa agcccgatct
      421 gatcccgcca gcggttgatg ttggacaaga ggccgcccgc gtcgccgtcc agcacggtca
      481 ccgacagatc gccggtccgg ccgtcgggcc ccggaaccac gaaagtggcg taacgaaagc
      541 ccgatccggg tttttcaatc catcctttcg gcgttttcca cgtcacgcga ttttcgggcc
      601 cggcgctcgg agcgatgccc gcggcctggg cgccgcccaa atccatgccc ggagctcccg
      661 aaatgtccgg gtgcccgggg ggcagagagg attttggctc ttcggcggtt tccgccggcg
      721 tgcccgggga cggcatggac gagggcgcgg cggccgggac ggaaagattc ggcgcgtcgg
      781 attccttggg gaccgtgtaa taagcgatgt cgtcccgacg gcacccggcc gcggccaagg
      841 aaagagtaat gacgatgagg gcggtggagg atttcattga aaagtgttca agcattatag
      901 aaatttaaga gtcgtctcca ataataggaa ttctatcggc ggggattacg aaatccactt
      961 gaattgcacc cccacggaca ctctggggaa tatttggagc acgtactagg aagggaactc
     1021 ccgcgac
//
LOCUS       DAOQLS010000883         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_143664_length_1027_cov_4.781893, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000883 DAOQLS010000000
VERSION     DAOQLS010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143664_length_1027_cov_4.781893"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK362_12275"
     CDS             complement(<1..273)
                     /locus_tag="PK362_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75648.1"
                     /translation="MRFNQGAGPALALAGLGLFSTLTFSAWMHIQAMRLSYRAQALRR
                     SIAEWERREQAAVRRSESTLSLARLDEAALSRFSLRLPRPDQLRFVR"
     gene            complement(595..>1027)
                     /gene="mraW"
                     /locus_tag="PK362_12280"
     CDS             complement(595..>1027)
                     /gene="mraW"
                     /locus_tag="PK362_12280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNC75649.1"
                     /translation="EAALRDAGEERFARKLAARLKEHAPEWATTKDLADAVCRWIPRR
                     GRSHPATRVFLGLRMAVNREMPRLRSMLERAPAVLRPGGRLAVITFHSTEDRVVKRYG
                     KEEWREGPLRAVNKKVLIASREEQRVNPRSRSAKLRVFEKI"
BASE COUNT          205 a          361 c          313 g          148 t
ORIGIN      
        1 gcggacaaaa cgaagctgat cggggcgtgg aaggcgaagg gaaaaccggg aaagggccgc
       61 ctcgtccaag cgggccaggg acaacgtcga ttcggaacga cggaccgccg cctgctcgcg
      121 ccgctcccat tccgcgatgg agcgacggag ggcctgcgcc cgataggaca acctcatcgc
      181 ctgaatgtgc atccaggcgg agaaagtcaa cgttgaaaac agccccagcc ccgcaagggc
      241 caaagccggt cccgcaccct gattgaagcg caccgcgcgc ctcccgttcc gttgaacggc
      301 gactcaaaat cgtccgggag agaggaagaa gggttcgcct tccccctctc ttcccggaag
      361 ttgcagcccg tggtgtcaaa gaggcaaggt gtctccgccg agagaagaca cccagcccaa
      421 atcgtttgca ccgcccgccg accgcgggcg cccaccggac acgaaaccgc gccgcgttgt
      481 tcaaaagggc gggtgcctcc gccgagagaa gacaccctgg ccccaaaacg tcgcccgcgc
      541 accaacgggg aacgccacga ctcggcgcgt tcgccgccgg ccctcgagca gggcctaaat
      601 tttttcaaac acccgaagtt tggcgcttcg actgcggggg ttgacccgct gctcctcccg
      661 ggaagcgatc agaaccttct tattgaccgc ccgcaaagga ccctcacgcc attcttcctt
      721 gccgtaccgc ttcaccaccc ggtcttcggt ggagtggaaa gtgatcacgg ccagccggcc
      781 gccgggtcga aggaccgccg gtgcacgctc gagcatcgag cggaggcggg gcatctcccg
      841 gttcaccgcc atccgaagac cgagaaaaac ccgggtggcg gggtggcttc gaccgcgtcg
      901 cggaatccat cgacagacgg cgtcggccaa atccttggtg gtcgcccact ccggcgcgtg
      961 ctccttcagc cgcgccgcca actttcgggc gaaccgttcc tcgcccgcgt ctcgcaaagc
     1021 cgcttcc
//
LOCUS       DAOQLS010000884         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_143783_length_1027_cov_2.435185, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000884 DAOQLS010000000
VERSION     DAOQLS010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143783_length_1027_cov_2.435185"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PK362_12285"
     CDS             <1..967
                     /locus_tag="PK362_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNC75650.1"
                     /translation="MPALEMDRATRRMGEDLVLDELFDLRLYERFRDRARGPLRGMLE
                     DLVVVEAGHYKFWKDFFGSDLQRLNPSRRVKLAVLSGICRVAGPAAVSLVLEGIEIYG
                     IRKYLELWDQYRDKPLGNAVRQILNDEMEHEDVIVSQFVSKKISPERVRTIFMGFNDG
                     LVEILGAVSGFFAALREPRLVLAASVSVAAAGAFSMAAGAYAATGSEREIERTQRRKD
                     AFLGRTAETGDGASPLSAAALVGLSYFLGASLPVLPVFFGATGVAISAVAAAAAVTVV
                     SAGLAFLSGMDMGRRVLLNLGIVAGAVAMTSLLGSLAHRWLGVSL"
BASE COUNT          148 a          332 c          359 g          188 t
ORIGIN      
        1 catgcccgcc ttggaaatgg accgcgcgac ccgccgcatg ggcgaggacc tcgtgctgga
       61 cgaattgttc gacctgcgcc tctacgagcg ttttcgggac cgcgcccggg gccccctgcg
      121 cggaatgctc gaagacttgg tcgtggtcga agccggccac tacaaattct ggaaggattt
      181 cttcggcagc gaccttcagc gcctgaaccc gagccggcgg gtgaaattgg ccgtgctttc
      241 ggggatctgc cgggtcgccg ggccggcggc ggtgtccctg gtgttggagg ggatcgagat
      301 ttacggcatc cgcaaatacc tggagctctg ggaccaatac cgggacaaac ccctcggcaa
      361 cgcggtgcgg cagatcttaa acgacgaaat ggaacacgag gacgtgatcg tctcccaatt
      421 cgtcagcaag aagatcagcc ccgaacgggt acggacgatt tttatgggct tcaacgacgg
      481 cctggtggaa atcctggggg ccgtgagcgg atttttcgcc gccctgcggg agccccgcct
      541 ggtcctggcc gcgagcgtgt cggtggccgc cgccggggcc ttttccatgg cggccggggc
      601 ctacgccgcg acgggctccg agcgggaaat cgagcggacc cagcggcgga aggacgcctt
      661 cctgggccgg accgccgaga ccggcgacgg agcgtcgccc ctttccgccg cggccctcgt
      721 gggcttgagc tactttttgg gcgcctccct gccggttctt cccgtgttct tcggcgccac
      781 cggcgtggcg atctcggccg tggccgcggc cgcggccgtc acggtcgttt ccgccgggct
      841 cgcctttttg tccgggatgg acatggggcg gcgggtcttg ctcaatttgg gcatcgtggc
      901 cggggccgtg gccatgacgt cgctgttggg atccctggcc catcggtggc tgggggtgtc
      961 cctgtgagcg cccccagcca ccgatgggcc agggatccca acagcgacgt catggccacg
     1021 gcccatc
//
LOCUS       DAOQLS010000885         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_143819_length_1027_cov_2.202675, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000885 DAOQLS010000000
VERSION     DAOQLS010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143819_length_1027_cov_2.202675"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1027)
                     /locus_tag="PK362_12290"
     CDS             complement(<1..>1027)
                     /locus_tag="PK362_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC75651.1"
                     /translation="HRALAPHGLFYAPDPASQKACTLGGNVGTNAGGPHCLKYGVTSH
                     HVTGLELVQPDGSVVQVSVDHPGFDLTGLFVGSEGTLGVATKIEVNLLPQPEDVRTFL
                     VAFPSMEAAAQTVTDIIGAGIVPATLEVMDRLTVQAVEAFVHAGYPTSAEAVLLIEVD
                     GPRDRTTLEGGRIRALCAKNKGSDFRMAVDEREREKLWEGRRGAYAAMARLAPNVLVE
                     DGVVPRTRLPEAVKKIRAIADRANLRMGLIAHAGDGNLHPNIIFDERDAAETSRVLGA
                     GQEMLRVCVDLGGSISGEHGIGADKREAMRWLFTPPTLSLFREIKRVVDPDNLCNPDK
                     LIPSADGN"
BASE COUNT          180 a          347 c          314 g          186 t
ORIGIN      
        1 gtttccgtcc gccgagggaa ttaatttgtc cgggttgcac aggttgtccg gatcaacgac
       61 ccgtttaatc tcgcggaaaa gggacagggt gggcggggta aagagccagc gcatggcctc
      121 ccgtttgtcg gcgccgatcc cgtgttcgcc ggagatggac ccgccgagat ccacgcacac
      181 ccgcagcatt tcttgcccgg cgcccagcac gcgcgaggtt tccgcggcat cccgctcgtc
      241 gaaaataatg ttggggtgga ggttgccatc cccggcgtgg gcgatgagcc ccatgcgcag
      301 gttcgcccgg tcggcgatgg cgcggatttt tttgaccgct tccggcaacc gggtgcgcgg
      361 caccacgccg tcttccacga gcacgttggg cgccaaccgc gccatggcgg cgtaggcgcc
      421 ccgccgccct tcccacagct tctcccgttc ccgctcgtcc accgccatgc gaaaatcgga
      481 gcctttgttt tttgcgcaaa gggcgcgaat gcgcccgcct tccagggtcg tccgatcccg
      541 cggaccgtcc acttcgatca acaacacggc ttccgccgag gtggggtagc ccgcatgcac
      601 gaaagcctcc accgcttgca cggtcaaccg gtccatcact tccaacgtgg cgggaacaat
      661 tccggcgcca atgatgtccg tcaccgtttg ggccgcggct tccatggagg gaaaggccac
      721 caaaaaggtc cgcacatcct cgggttgcgg cagaaggttc acttcgattt tcgtggccac
      781 acccaaggtt ccctcggatc ccacgaacag gccggtcaag tcgaacccgg gatgatccac
      841 ggacacctga accacggacc cgtccggctg aaccaattcc agcccggtga cgtggtgact
      901 ggtcaccccg tatttcaaac aatggggccc gccggcgttc gtgcccacat tgccccccag
      961 ggtgcaggcc ttttggctgg ccggatcggg ggcatagaag aggccgtggg gcgccaaggc
     1021 ccgatgc
//
LOCUS       DAOQLS010000886         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_144023_length_1026_cov_2.938208, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000886 DAOQLS010000000
VERSION     DAOQLS010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144023_length_1026_cov_2.938208"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PK362_12295"
     CDS             complement(<1..892)
                     /locus_tag="PK362_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75652.1"
                     /translation="MSELSLVQGDDSYFIARRREKTLTLSGISAAVPSADQPPPLRRL
                     KVRLWAPLPAKALAGGGTGADIIVRPVSQTERMVKSYGKALDRIPAPLAARLEGVRSA
                     MILGPGANADEIELLWRRLPHVREIHLVEVVADYIKAQDDFLVQHPDWAVNADGDPIA
                     YYAWRAPMGALPVELNGSMDFLFDHQVLDFGYLGRLGMETVARSLNRVLRPSGLHVSF
                     VFGSEPAMDEYLGPALFRDETLALALRGPPSVPALPKVPPAGVSSQAEDFLNLVRATP
                     APGAVLSLGFGAGDDERYLAR"
BASE COUNT          190 a          330 c          355 g          151 t
ORIGIN      
        1 gccgggccag atagcgttcg tcgtcgccgg cgccgaaccc gaggctcagc acggcgccgg
       61 gcgccggggt ggcgcgcacc aggtttagaa aatcctccgc ctgggaggaa acgcccgcgg
      121 gcgggacctt cggcagggcg ggaacgctcg gcggcccgcg aagggccaac gcaagggtct
      181 cgtcccggaa gagggcgggc ccgaggtatt cgtccatggc gggttcggaa ccgaaaacga
      241 aactcacgtg gagcccgctc ggccgcagga cgcggttcag ggaccgggcg accgtttcca
      301 ttcccagtcg gcccaggtac ccgaaatcca gaacctggtg atcgaagagg aaatccatcg
      361 acccgttcaa ctccaccggg agggcgccca tgggcgcgcg ccaggcgtaa taggcgatcg
      421 ggtctccgtc ggcgttcacc gcccaatcgg gatgctgaac gagaaaatcg tcctgggctt
      481 tgatgtaatc ggcgacgact tccaccagat ggatttcccg gacgtggggc aggcgacgcc
      541 acaggagttc gatttcatcg gcgttggccc cgggaccgag gatcatcgcg cttcggacgc
      601 cttccaaccg ggccgccaag ggcgcgggaa tccggtcgag ggccttcccg taggatttca
      661 ccatccgctc ggtctgggaa acgggccgga cgatgatgtc ggccccggtt cccccgcccg
      721 ccagcgcttt cgccggaaga ggcgcccaca aacggacttt aagccggcgg agcggcggcg
      781 gttggtccgc ggacggaacg gcggccgaaa tccccgacag cgtcaaggtc ttttcccgcc
      841 ggcgggcgat gaagtaactg tcgtccccct ggaccaggga caattcgctc acgttccatt
      901 ccccgaaaac atcgaaccga tggcggagga cccaggctcg tagggcttga agttccgcgg
      961 gggcaagggg tcgacggggc gaaaagaggg cgatggaccc ccgcgtaccg tccgatcgtt
     1021 cccggt
//
LOCUS       DAOQLS010000887         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_144585_length_1024_cov_3.642931, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000887 DAOQLS010000000
VERSION     DAOQLS010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144585_length_1024_cov_3.642931"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PK362_12300"
     CDS             complement(<1..677)
                     /locus_tag="PK362_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC75653.1"
                     /translation="MSRVVLQNVSKWYGLVPAVQNVSLEVEDRELFVLVGPSGCGKTS
                     LLRLIAGLDDVSDGKILIDRRVVNHDAPKSRNIAMVFQNFALYPHMTVFDNMAFGLRM
                     RGYSDEEVSARVRQASDMLELTPLLDRKPNEISGGQRQRVAVGRAIVRKPRVFLFDEP
                     FSNLDAKMRAETRGELLRLHQRLQTTMIYVTHDQVEAMTMGDRIAVLKDGAVQQVGTP
                     LDLYRRP"
     gene            complement(680..>1024)
                     /locus_tag="PK362_12305"
     CDS             complement(680..>1024)
                     /locus_tag="PK362_12305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC75654.1"
                     /translation="QMVRRLAHPGVTLGRFGGDEFALIMEMDSQVEVFAWFERLRQLT
                     AESPLRDREALIRFTISAGVSAYLPGRPALSDLMAQADHALYQAKRAGRDRVVAWRAP
                     GSTSSSTAPVKG"
BASE COUNT          198 a          327 c          322 g          177 t
ORIGIN      
        1 ttgggccgcc ggtaaaggtc caggggcgtg cccacttgtt gaacggcgcc gtccttcaac
       61 accgcgatgc ggtcgcccat ggtcatggct tccacctggt cgtgggtcac gtaaatcatg
      121 gtcgtctgaa gccgctggtg aaggcgcagg agctcgcccc gggtttcggc ccgcattttg
      181 gcgtccagat tggaaaacgg ctcgtcaaac agaaacaccc ggggtttgcg aacaatggcc
      241 cggcccaccg ccacccgctg ccgctgaccg ccggatattt cgttgggttt gcggtccaga
      301 agcggggtca gttccaacat gtccgaggcc tggcgaaccc gggcggaaac ctcctcgtcc
      361 gaatagcccc gcatgcgcag accgaaggcc atgttgtcga acaccgtcat gtggggatag
      421 agggcgaaat tctggaaaac catggcgatg tttcgggact tgggcgcgtc gtggttcacc
      481 acgcggcggt cgatcagaat tttcccgtcc gagacgtcgt ccaagccggc aatcaggcga
      541 agcagggacg tcttgccgca tcccgagggt cccaccagca caaacaattc ccggtcttcc
      601 acttccaggc tcacgttctg cacggcgggc accaggccgt accatttgga cacgttttga
      661 agaacgacgc gggacatggt taacctttca ccggcgcggt ggaggaagac gtcgacccgg
      721 gggcgcgcca cgccacgacc cgatcgcgtc cggcccgttt ggcttggtac aaggcgtggt
      781 cggcctgggc catcaaatcg gacagcgccg gccgaccggg aaggtaggcg ctcacgccgg
      841 cggagatcgt gaaccgaatc aaggcctccc ggtcccgaag gggcgattcc gccgtcagct
      901 gacgcaggcg ttcaaaccag gcgaacacct ccacctggga atccatttcc atgatcagag
      961 cgaattcatc gccgccgaag cgccccaacg ttacccccgg gtgggcgagg cggcgcacca
     1021 tctg
//
LOCUS       DAOQLS010000888         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_144596_length_1024_cov_3.399381, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000888 DAOQLS010000000
VERSION     DAOQLS010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144596_length_1024_cov_3.399381"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PK362_12310"
     CDS             <1..908
                     /locus_tag="PK362_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75655.1"
                     /translation="QLLSSLPNLNDYTLLANGGWNANWYVGHNTCWVHKLPAASSGPY
                     VRAFVGAKLGAMKTEPIPGRPTWERRVIPGEVAIALAPEPSWSQSKRYLLAMSEDIPL
                     EGDPDNALEGVGEARWFWVEVPLKSISTTGDNYVALYSPDDEFRDARHAPILAAGPSN
                     GVVDTWLNNSVRGQPPLTPVEALKTPVVSYEPAVALKLVPAREERPRVAWRRPPAKDA
                     EINPRLVLEAEVSGSDIESAWVEFSTDSRSWRRMGRREYGAPYAFTLTRETLPKGPVQ
                     VRAVAQDTWENAGASPVLVLKVPRK"
BASE COUNT          181 a          357 c          312 g          174 t
ORIGIN      
        1 cccagctgtt gtcgtccctt cccaacctca acgattacac cctcctggcg aacggcgggt
       61 ggaacgccaa ttggtacgtc ggccacaaca cctgctgggt ccataaattg ccggccgcct
      121 cctcgggccc gtatgtccgg gccttcgtgg gcgccaaatt gggcgccatg aaaacggagc
      181 ccattcccgg acgtccaacc tgggaacggc gggtgatccc cggcgaggtg gccatcgccc
      241 tggcccccga accgtcctgg tcccagagca aacggtattt gctggccatg tccgaggaca
      301 ttcccctgga gggcgacccg gacaacgcgc tggaaggcgt gggcgaagcc cgctggttct
      361 gggtcgaagt gcccctcaaa tcgatttcaa cgaccggcga caactacgtg gcgctctatt
      421 cgcccgacga cgaatttcgg gacgcccgcc acgcgccgat tctggcggcc ggtcccagca
      481 acggcgtcgt ggacacctgg ctcaacaatt cggtgcgcgg gcaaccgccg ttgacgccgg
      541 tcgaggccct gaaaacgccg gtggtgtcct acgagcccgc cgtggcgttg aaactggtcc
      601 ccgcccggga agagcgcccc cgggtggcct ggcgccgtcc ccccgcgaaa gacgccgaaa
      661 tcaatccccg attggtgctg gaagccgagg tttccggttc cgacatcgaa agcgcctggg
      721 tggagttctc gacggattcc cgttcctggc ggcggatggg ccgccgggaa tacggagccc
      781 cctacgcttt caccctgacc cgggaaaccc tgcccaaggg acccgtgcag gtccgggccg
      841 tcgcccagga cacctgggag aacgccggcg cctcgcccgt tttggttttg aaagttcccc
      901 gaaaataacg gaggtcccat gaaacgactt gttctgcttt tgccttttct cgccgcctgc
      961 acctccaagc cgaaggaggc cgccgctccc gcgcccaagg tagacaacac cgtggtccgc
     1021 taca
//
LOCUS       DAOQLS010000889         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_144864_length_1023_cov_3.538223, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000889 DAOQLS010000000
VERSION     DAOQLS010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144864_length_1023_cov_3.538223"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PK362_12315"
     CDS             <1..>1023
                     /locus_tag="PK362_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNC75656.1"
                     /translation="GRFGRKPEGLWLAETAVDTETLNVLIEHGIRFTLLSPQQARAVR
                     PARRSADAESPESAWRDVSGGRIDPSRPYLWRSPAGGTIVLFFYDAPISRAVAFEGIL
                     NNGETFAARLTDGFSNDRQEPQLVHIATDGESYGHHHRFGDMALAYALKKLEQDRRLS
                     LTNYGEFLDHHPPNWEVQIHEASSWSCPHGVERWRSNCGCRMGSHAHWNQEWRTPLRD
                     SLNELAADLDRLFESRGADLFVDPWRARNAYIDVVLDRGPETLEGFWKSQTRRPLSPA
                     EQSQALKLLEMERHRLLMFTSCAWFFDEISGLESTTVLLSTARALQLAAQFPGAEGLE
                     ESFLNRL"
BASE COUNT          183 a          360 c          306 g          174 t
ORIGIN      
        1 cggccggttc ggccggaaac ccgaaggcct gtggctggcc gaaacggccg tggacacgga
       61 aaccctgaac gtgctcatcg aacacggcat tcgattcacc ctcctctccc cgcaacaagc
      121 ccgggccgtc cgccccgccc gtcgctcggc cgacgccgaa agtccggaat ccgcctggcg
      181 ggacgtgtcg gggggccgga tcgaccccag ccgcccctat ttgtggcggt cgccggccgg
      241 cggcaccatc gtcctctttt tttacgacgc tcccatttcc cgggcggtcg cctttgaagg
      301 catcctgaac aacggcgaaa cgttcgccgc ccggctgacc gacggctttt cgaacgaccg
      361 tcaagaaccc caactcgtgc acatcgcgac cgacggggaa tcctacggcc accaccaccg
      421 gttcggcgac atggccctgg cctacgcgct gaagaaactg gagcaggacc gccggctttc
      481 gctcaccaac tacggcgaat ttttggatca ccaccccccg aactgggagg tgcaaatcca
      541 cgaggcgtcc tcctggagtt gcccccacgg ggtggaacgc tggcggtcca attgcgggtg
      601 tcgcatggga tcccacgccc actggaacca ggaatggcgg acccccctgc gggattccct
      661 gaacgaactg gccgcggacc ttgaccgtct gttcgaatcc cgcggcgcgg atttattcgt
      721 cgacccctgg cgcgcccgga acgcgtacat cgacgttgtc ctcgaccgcg gacccgagac
      781 gctggagggg ttctggaaat cccaaacccg gcggccgttg tcccccgcgg aacaatcgca
      841 ggcgctgaag ctcctggaaa tggagcggca tcggctgctc atgttcacaa gctgcgcctg
      901 gtttttcgac gaaatctccg gactggaaag caccacggtc ctcctgtcga cggcccgggc
      961 tctgcaactg gccgcccaat tccccggggc ggaggggctt gaagaatcct ttttgaaccg
     1021 gct
//
LOCUS       DAOQLS010000890         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_144945_length_1023_cov_2.451446, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000890 DAOQLS010000000
VERSION     DAOQLS010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144945_length_1023_cov_2.451446"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..>1023)
                     /locus_tag="PK362_12320"
     CDS             complement(84..>1023)
                     /locus_tag="PK362_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC75657.1"
                     /translation="LPRLIGRPLHEAFDSPSPEALEKAIAEAAASHQIQTAEIQCGVC
                     SLDRFYEARIVPSAPGELMAIVRDITDRKRWEKAAVESAKLSAMGRLAGGVAHEINNP
                     LGIILGFAQALKEQWSAEESIRHPLAFIEKEVLRCKALVQNLLIYTRKSKPEEREALD
                     LSRVTEEALTLVMAQGRVKDVELVRNLKSDVPVWGNKNQIQQIVLNLCNNAMDAMAGG
                     GTLIVSTERIALGDRPGGQLSVADTGIGVPEAIRSKIFEPFFTTKEVGRGTGLGLALV
                     HEIVQNHSGEIALTSVEGAGTTFFVRFPAGETGPGE"
BASE COUNT          180 a          336 c          291 g          216 t
ORIGIN      
        1 ccttcccccc cctgcccgac ggcttcgaag ccaaggacct tccggtcctg gtccgtttcc
       61 gcctccagta gttcgtcggg ccctcattcg cccggcccgg tttctcccgc cggaaatcga
      121 acaaaaaaag tagtccccgc cccctccacg ctcgtcaaag cgatttcccc gctgtggttt
      181 tgaacgattt cgtgcaccag ggccaacccc agccccgttc cgcggcccac ctctttggtc
      241 gtaaaaaaag gctcgaagat tttggatcgg atcgcctcgg ggacgccgat gcccgtgtcg
      301 gccacgctca attggccacc gggccgatcg ccgagggcga tccgttccgt gctcacgatc
      361 agggtgccgc ccccggccat ggcgtccatg gcgttgttgc agaggttcag cacgatttgc
      421 tgaatttggt tcttgttccc ccacacggga acgtcggact tcaagttccg aaccaattcg
      481 acgtccttga cccgcccctg ggccatgacc aaggtcaaag cctcttcggt cacccgactg
      541 aggtccaggg cttcccgttc ttcgggtttg gatttgcgcg tgtaaatcag taggttttga
      601 accaacgcct tgcaacggag aacttccttc tcaataaagg ccaacggatg acggatggat
      661 tcctccgccg accactgttc cttgagcgcc tgggcgaacc ccaaaatgat ccccaggggg
      721 ttgttgattt catgggccac gccgccggcc aagcggccca tggccgacag cttggccgac
      781 tcgacggccg ccttttccca tcgtttccgg tccgtgatgt cccggacgat ggccatcagt
      841 tcgccgggag cggaggggac gattcgggcc tcgtaaaatc gatccagcga acaaacgccg
      901 cattgaatct ccgctgtttg gatttggtgg gaggccgcgg cctccgcaat ggctttttcg
      961 agggcttccg gcgacgggga atcaaacgct tcgtgaaggg gccgcccgat gaggcgcggc
     1021 agg
//
LOCUS       DAOQLS010000891         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_145125_length_1022_cov_3.853154, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000891 DAOQLS010000000
VERSION     DAOQLS010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145125_length_1022_cov_3.853154"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1022)
                     /locus_tag="PK362_12325"
     CDS             complement(<1..>1022)
                     /locus_tag="PK362_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNC75658.1"
                     /translation="GVSPTRAPIPALLAVAGLHHDMIRKGTRMKVGLLLESGEPREVH
                     HFATLLGYGAGAINPYMAYETFDDMIDQGLLVGVDHKTAVKNFVKAATKGVIKTLSKM
                     GISTIQSYRGAQIFEAIGLAPSVVDKYFTSTPTRVGGADLAVVAEEARRRHHRAFPDR
                     VTDGKVLDVGGEYQWRKEGEAHLFNPETIHRLQLAARTNNYATYKSYAEIVNKQEKTL
                     YTLRGLLGFKPATPIPLDEVEPVEAIVKRFKTGAMSYGSISQEAHESLAIAMNRLGGK
                     SNTGEGGEDPARFTPLADGDSKRSSIKQVASGRFGVTSEYLVNADDIQIKMAQGAKPG
                     EGGQLP"
BASE COUNT          147 a          306 c          356 g          213 t
ORIGIN      
        1 cgggcagctg gccgccctcg ccgggcttgg cgccctgggc catcttgatc tggatgtcgt
       61 cggcgttgac caggtactcg ctggtgacgc cgaagcggcc ggaggccacc tgcttgatcg
      121 acgagcgctt ggagtccccg tcggcgagcg gggtgaagcg cgccgggtcc tcgccgccct
      181 cgccggtgtt ggacttgccg cccaggcggt tcatggcgat ggccagggac tcgtgggctt
      241 cctgcgaaat ggagccgtaa ctcatggcgc cggttttaaa tcgtttcacg atggcctcga
      301 ccggttcgac ttcgtcgagc ggaatgggcg tggcgggctt gaaacccaaa aggccccgca
      361 gggtgtatag ggttttttcc tgcttgttca cgatctccgc gtaggatttg taagtcgcgt
      421 agttgttcgt gcgggccgcc agttgcaaac ggtggatggt ttcggggttg aagaggtgcg
      481 cttcgccctc cttccgccat tggtattcgc cgcccacgtc caggaccttg ccgtcggtga
      541 cccggtccgg gaaggcgcgg tggtggcgtc ggcgggcctc ttcggcgaca accgccaaat
      601 cggcgccccc cacgcgggtg ggcgtgctgg tgaaatattt atcgacgacc gacggcgcca
      661 gtccgatggc ctcgaaaatc tgggcgccgc ggtagctttg gatggtcgaa atgcccatct
      721 tggagagcgt tttgatgacg cccttggtgg cggccttcac gaaattcttc acggccgttt
      781 tgtggtccac gcccaccagc aggccctgat cgatcatgtc gtcgaaagtt tcgtacgcca
      841 tgtaggggtt gatggccccg gcgccgtagc cgaggagcgt tgcgaagtgg tggacctccc
      901 ggggctcgcc gctttccagg agcagcccga ctttcatgcg ggtgcccttg cggatcatgt
      961 cgtggtgcaa cccggccacg gccaggaggg ccgggatcgg ggctcgggtc ggcgaaacgc
     1021 cc
//
LOCUS       DAOQLS010000892         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_145869_length_1020_cov_2.212435, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000892 DAOQLS010000000
VERSION     DAOQLS010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145869_length_1020_cov_2.212435"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PK362_12330"
     CDS             complement(<1..293)
                     /locus_tag="PK362_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75659.1"
                     /translation="MNGRRFRSPHRPLSDVFRSALRAGVAAAVFLTSFGPLGAENWWT
                     DRRRAAHSRVAEAAPATESLGAVLPAAEEAWSARVFGRAEGAAAPLLPAWIRST"
     gene            complement(368..>1020)
                     /gene="coaE"
                     /locus_tag="PK362_12335"
     CDS             complement(368..>1020)
                     /gene="coaE"
                     /locus_tag="PK362_12335"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNC75660.1"
                     /translation="KGFCMSSTATTSAPGGIGSIERAQLIMVPIISGRPFFWKPAAAA
                     FRARRIPVVDADQIVRRALGPRGAARSRVARLFGPGVVQGSGLDRKKIAAAVFSNPSL
                     RRRLEGILHPLVAREIRRAIARRPALLVLDVPLLFESGLDRLADATLVIGAPWALRRR
                     RLVRGGRMTASDARRRDAAQMPLAAKKRRADFVIDNRGSRADLDREIRRFLEAIKK"
BASE COUNT          192 a          326 c          361 g          141 t
ORIGIN      
        1 gtggagcgga tccaggccgg caacaacggg gccgccgcgc cttcggcgcg gccgaaaacc
       61 cgggcgctcc aggcctcctc ggcggccggg aggacggcgc cgagggattc cgtggccgga
      121 gcggcctccg ccacgcgcga atgggccgca cgccgacgat ccgtccacca attttcggcg
      181 cccaggggcc cgaaagaagt taagaaaacg gcggcggcca cccccgcccg gagggcggac
      241 cggaagacat cggaaagggg gcgatggggg gatcggaacc gtcggccgtt catagagctt
      301 tagtatagac gccccgggcg gaagaaccgg ggaagggatt gtaaaggttt tgtaaaagag
      361 ggcgtattta tttttttatg gcctctagaa atcgtctgat ttcccgatcc aaatccgctc
      421 gggaccccct gttgtcgatg acaaaatccg cgcgccgttt tttggccgcc aggggcattt
      481 gggcggcgtc ccggcggcgg gcatcggagg ccgtcatccg gccgccgcga accagccgcc
      541 ggcgccggag agcccagggg gcgccgatca ccagggtggc gtcggccaaa cggtccaggc
      601 cgctttcaaa aaggaggggg acgtccagca ccaacagggc ggggcggcgg gcgatggcgc
      661 gtcgaatttc ccgggccacc agggggtgaa gaattccctc cagacgccgc cgcagggacg
      721 gattggaaaa gaccgccgcg gcgatttttt ttcgatccaa accggaccct tgaacgacgc
      781 cggggccgaa aaggcgcgcc acccgggaac gggcggcgcc gcgcggcccg agggcccggc
      841 ggacgatttg gtccgcgtcc accaccggaa ttcgccgggc ccgaaacgcc gcggcggcgg
      901 gcttccaaaa gaaaggccgc cccgatataa tcgggaccat gatcaactgc gccctctcga
      961 tgctgccgat ccctccaggg gcggaggtgg tggcggtgct gctcatgcag aaccccttcc
//
LOCUS       DAOQLS010000893         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_145898_length_1020_cov_1.986528, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000893 DAOQLS010000000
VERSION     DAOQLS010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145898_length_1020_cov_1.986528"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..>1020)
                     /locus_tag="PK362_12340"
     CDS             complement(187..>1020)
                     /locus_tag="PK362_12340"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC75661.1"
                     /translation="LVIDRYGDVCVIESFAAGMDRLLPFILEAVKSFGPWKAIVLRND
                     ASARRLEHLPEDVRVLDGALETPHWFTTDGIVMAADPLQGQKTGFFFDQRVNRAAVAA
                     LAPGRHVLDVFCHTGGFALWCAKAGAKSVRGVDVSAAALDLARLTAEKNGWADRCDWQ
                     KADAFEHLAHLKETFDIVVLDPPRFAPAKKNLPDAIKAYVRLNALGLKRVAGGGFLAT
                     ASCSQHVSRDDFRQIISRAGHESGRKVKIVYQGGPGPDHPVRPSMPETDYLKFAVLYV
                     S"
BASE COUNT          230 a          325 c          294 g          171 t
ORIGIN      
        1 agccccgcta tcggcgaaaa atcgtgctca aaacgcctca atgggggagc gaaagaataa
       61 aaccactaaa ccccacaaaa accaggggca acgccgccat accccgaagt cccttattga
      121 aagtttttcc ctctcccctt gggagagggc agggtgaggg attgaatgtt ggtaaaaacc
      181 agccgttcag gaaacgtaca aaaccgcgaa cttgagataa tcggtttccg gcatggacgg
      241 ccgaacagga tggtccggcc cgggcccgcc ctgataaacg attttgactt tccgaccgga
      301 ctcatgacca gcgcgggaaa tgatctggcg gaaatcgtcc cggctgacat gctgggaaca
      361 gctcgccgta gccaaaaatc cgccgccggc cacccgtttc aacccgagcg cgttcaaccg
      421 cacataggcc ttgatcgcgt cgggcagatt tttcttggcg ggggcgaacc gcggcggatc
      481 caacaccacg atgtcgaagg tctctttcaa atgggcgaga tgctcgaacg cgtcggcctt
      541 ctgccaatcg caccggtcgg cccagccgtt tttttccgca gtcagacggg ccaggtccaa
      601 ggccgccgcg gagacgtcca cgccgcgaac gcttttggcc ccggccttgg cgcaccagag
      661 ggcaaacccg cccgtatggc aaaacacgtc caacacatgg cgccccggcg ccagggcggc
      721 cacggccgcg cggttcaccc gctggtcaaa gaaaaatccg gtcttctgcc cttgcagggg
      781 atcggcggcc atgacgattc cgtccgtcgt gaaccagtgg ggcgtttcaa gggcgccgtc
      841 cagtacccgc acgtcttccg gcaaatgttc gagccggcgg gcggacgcgt cgttgcgaag
      901 cacaatggcc ttccacgggc cgaaactttt caccgcctcg agaatgaacg gaagcaaccg
      961 atccatcccg gcggcgaacg actcgatcac gcaaacatcg ccgtatcggt cgatgacgag
//
LOCUS       DAOQLS010000894         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_146058_length_1019_cov_3.092324, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000894 DAOQLS010000000
VERSION     DAOQLS010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146058_length_1019_cov_3.092324"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PK362_12345"
     CDS             complement(<1..1008)
                     /locus_tag="PK362_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNC75662.1"
                     /translation="MNALPKTVKQLVVLDKTKEPGSQGEPLYLDVSSAIMESVMENRL
                     PFAMPRIYGGRYGLSSKEFNAGMIKGLYDHLKAGTMKNHFTIGINDDVSHTSLSYDPN
                     FIVEDKEIIRAVFWGLGGDGTVSANKNSIKIIGEDTPNYAQGFFFYDSKKAGAITISH
                     LRFGKKPIRSAYLIQKANFVACHDYQFMGKYDVLKYAEEGATFLLNSPLPADKVWDDL
                     PAEAQKKIIERKVKFYVLDGYAVAKQVGLGRRFNIPLQTAFFALANILPKEEAVKHIK
                     EYIEKTWTKKGEDVVRMNKAAVDHALETLTEVKYPNKVTSTKSMQPAVTSNAPDFVQS
                     VT"
BASE COUNT          185 a          254 c          322 g          258 t
ORIGIN      
        1 cgtgacgctt tggacgaaat ccggcgcgtt ggaagtgacc gcgggctgca tggacttggt
       61 ggacgtgact ttgttcgggt atttcacttc cgtcaaggtt tccaaggcgt gatccacggc
      121 ggccttgttc attcggacga cgtcctcgcc ctttttggtc caagtctttt cgatgtattc
      181 cttgatgtgc ttgacggctt cttccttggg aaggatgttg gccagggcga agaacgccgt
      241 ctgcaggggg atgttgaacc ggcggcccag gcccacttgc ttggccacgg cgtagccgtc
      301 caacacgtaa aacttcactt tccgctcgat gatttttttc tgggcctccg ccggcagatc
      361 gtcccagact ttatcggccg ggagcgggct gttaaggagg aacgtggcgc cttcctcggc
      421 gtatttcaag acgtcgtatt tgcccatgaa ctgatagtcg tggcaggcga cgaaattggc
      481 cttttggatc aggtacgcgg aacggatggg ctttttgccg aagcgcaggt ggctgatggt
      541 gatggcgccg gcctttttgg agtcgtagaa gaaaaagccc tgggcgtaat tgggcgtgtc
      601 ctcgccgatg attttgatgg agtttttgtt ggcggacacc gtcccgtcgc cgcccaagcc
      661 ccagaagacc gcccggatga tttctttgtc ctcaacgatg aaattggggt cgtagctcag
      721 gctggtgtgg gaaacgtcgt cgttgatgcc gatggtgaaa tggttcttca tggtgccggc
      781 cttcaggtgg tcgtagaggc ccttgatcat gccggcgttg aattccttgg aggacaggcc
      841 gtagcgaccg ccgtaaatgc ggggcatggc gaaaggaaga cgattctcca tcacactttc
      901 catgatggcg gaggacacgt ccagataaag gggttcgccc tgggagccgg gttctttggt
      961 tttgtccaaa acgaccaact gcttgacggt cttgggcagg gcgttcacga aatactctg
//
LOCUS       DAOQLS010000895         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_146223_length_1019_cov_1.542531, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000895 DAOQLS010000000
VERSION     DAOQLS010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146223_length_1019_cov_1.542531"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1019
                     /locus_tag="PK362_12350"
     CDS             <1..>1019
                     /locus_tag="PK362_12350"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNC75663.1"
                     /translation="SGVSSNGTLLPNSALVFGGSGANRTVVLTPALNQNGTTTLTLTV
                     TDGGGLTAQTAFKLTVTAVNDPPVALAQSLGVPSNTPKLIHLGGTDVDGDTLSYAYAQ
                     PSHGSVTGTAPLVTYTPNNGYVGFDTFTFTASDGLLPSPSASIVLQLDDTPPAVSITQ
                     PAEGATVMYTAALSAAATDNQSVDRVLFYADGVAIATATSETSAFTAGWDTSSAANGS
                     HVLTARAYDNAGNTALSSAVTVTVDNPALAQLAVAPGSLGFYGVEGGPVPPVSPLTLS
                     TASAGGSPSWTISVSTPWLSVSPLSGRGPNTVSVGVSPVGLSTGVFSEPGKGELVTLG
                     AGPAN"
BASE COUNT          160 a          396 c          281 g          182 t
ORIGIN      
        1 tgtccggcgt ctccagcaac gggacgctcc tccccaattc cgcactcgtg ttcggcgggt
       61 ccggcgccaa ccgaacggtc gtgctgacgc ccgccttaaa ccaaaacggc acgaccaccc
      121 tcacgttgac ggtgaccgac ggcggaggcc tgaccgccca aaccgctttt aaactcaccg
      181 tgaccgccgt gaacgatccg cccgtggccc ttgcccaatc cctcggcgtt ccctccaaca
      241 cccccaaact gatccacctc gggggcaccg acgtggacgg cgacaccctc tcctacgctt
      301 acgcccaacc cagccatgga tcggtcaccg gcaccgctcc gctggtcaca tatacgccga
      361 acaacggcta tgtgggattc gacaccttca ctttcacggc gtcggacgga ctcctcccca
      421 gtccttccgc gtcgattgtt ctacagctcg acgacacgcc gcccgccgtt tcaatcaccc
      481 aacccgcgga gggggcgacg gtcatgtata cggcggctct gtcggccgcc gcgaccgaca
      541 atcaatccgt ggatcgggtc ctcttttacg cggacggcgt tgcgatcgcc acggccacga
      601 gcgagaccag cgccttcacc gccggctggg acacgtcttc cgccgcgaac ggatcccatg
      661 ttcttaccgc ccgggcgtac gacaacgccg gcaacaccgc cttgtcctcc gcggtgaccg
      721 tcacagtcga caatcccgcc ttggcccagt tggcggtggc ccccggctcc ctgggcttct
      781 acggagtgga aggcggcccg gttccccccg tgagcccttt gactctttcg accgcctccg
      841 ccgggggatc gccctcttgg acgatcagcg tcagcacccc ctggctgtcc gtttctcccc
      901 tttcgggccg cgggccgaac acggtttcgg tgggcgtgtc tcccgtgggc ctttccaccg
      961 gcgtcttctc tgaacccggc aagggcgagc tggtcaccct cggtgccggc ccggcaaac
//
LOCUS       DAOQLS010000896         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_147154_length_1015_cov_4.790625, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000896 DAOQLS010000000
VERSION     DAOQLS010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147154_length_1015_cov_4.790625"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PK362_12355"
     CDS             complement(<1..142)
                     /locus_tag="PK362_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNC75664.1"
                     /translation="MPWLDLTIAGFFEVFWAVCLKYAQGFTRLGPSVASVAGMVLSVW
                     FLS"
     gene            complement(183..470)
                     /locus_tag="PK362_12360"
     CDS             complement(183..470)
                     /locus_tag="PK362_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75665.1"
                     /translation="MKLYVDDVRTPPAGWRLARTVAEAQALLERETVEEVSLDYYIGD
                     GEGGTFLPVAETIAALPEDRRPRRVRLHSASDAGAARMARVLSGRVPEIIR"
     gene            complement(467..>1015)
                     /locus_tag="PK362_12365"
     CDS             complement(467..>1015)
                     /locus_tag="PK362_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNC75666.1"
                     /translation="TRGRPTSAAPPAVIDVSLVPQGEGLNADDPDRLPTVPVLSVVDG
                     DTLEILWNDRPTKLRYYGVNTTERDQACYREGTDLNRALSGGAVRLAFDERTEDKYGR
                     LLAYAFTPEGKSIDAALVAAGMGKAWRRDGRFREQIVSLEDDARLHRRGCLWGVDRPG
                     GTAPSGAKPRTTRSRRSRRAKP"
BASE COUNT          175 a          347 c          349 g          144 t
ORIGIN      
        1 gggacagaaa ccaaacgctc aggaccattc ccgccacgga agcgacggag ggccccaatc
       61 gggtgaaacc ctgggcgtat ttcaagcaca cggcccaaaa cacttcaaag aatccggcga
      121 tggtgagatc cagccacggc atgggaaaaa gaatactcgc ccgggacccg gggagcaagg
      181 ggtcagcgaa tgatttccgg aacccgaccc gacaagaccc gggccatccg agcggccccg
      241 gcgtcggacg cgctgtgaag ccgcacccgg cggggacgac ggtcctcggg cagggcggcg
      301 atggtttcgg ccacgggcag gaaggtgccg ccctcgccgt cgccgatgta atagtccagg
      361 ctcacttctt ccacggtttc ccgttccaga agggcctggg cctccgccac ggtccgcgcc
      421 aggcgccagc cggccggggg cgttcgaacg tcgtcgacgt acagcttcac ggtttggcgc
      481 ggcgggagcg ccgcgaacgc gtcgttcggg gcttcgcccc cgaaggcgcg gttcccccgg
      541 gcctatcgac gccccagagg cagccgcgcc gatggagccg ggcgtcgtcc tccagggaaa
      601 cgatctgctc ccggaagcgt ccgtcccgcc gccacgcttt gcccatcccg gccgccacca
      661 acgcggcgtc gatggatttc ccctcggggg tgaaggcgta ggccaggagg cggccgtatt
      721 tgtcctccgt gcgctcgtcg aaagccagcc ggacggcgcc gcccgacaac gcccggttca
      781 ggtcggtccc ttcccggtaa caagcctggt cgcgctcggt ggtgttgacc ccgtaatagc
      841 gcaatttcgt gggccggtcg ttccaaagaa tttcgagggt gtccccgtca accacggaca
      901 gcacgggcac ggtgggcagg cggtcgggat cgtcggcgtt caacccctcc ccctgaggga
      961 cgagggacac gtcgatcacc gcgggcgggg cggcggaggt cggacggccc cgggt
//
LOCUS       DAOQLS010000897         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_147197_length_1015_cov_3.316667, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000897 DAOQLS010000000
VERSION     DAOQLS010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147197_length_1015_cov_3.316667"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK362_12370"
     CDS             <1..192
                     /locus_tag="PK362_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75667.1"
                     /translation="ERKDLTASVRALLRLQGENVLIIVRSDENVSYGDLTDILKEIKD
                     AGAKNIAVGTQLIQDKAGS"
     gene            254..>1015
                     /locus_tag="PK362_12375"
     CDS             254..>1015
                     /locus_tag="PK362_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75668.1"
                     /translation="MRHWEMLYDRQPLGLDAALFGVALLLHAPLLLIQFKAAPHSHRA
                     EPRVVKVELLENLNTQVFSNPISVRPTTGPVIKSPIDMPQYKDLIKRIQEARLKGDLL
                     HVVKPHVVPPAIPKAGLGTGPIKLPDAGLNNKGAFNHVAAPQLGSGPAGLKGGSAAAG
                     PAATGRPLGDIGGGPAGPALLGNKGSFSIRSGQIGSIGSGEGIKVGAGGAGAIAIKTT
                     KTGSVDDAVLDPIPVQDKGRFGALGGGDGSGGTLAG"
BASE COUNT          194 a          313 c          326 g          182 t
ORIGIN      
        1 gagcgcaagg atttgacggc ctcggtgcgg gcgcttctcc gtcttcaagg ggaaaatgtt
       61 ttaatcatcg tccgttccga cgaaaacgtg agctacggcg acctgacgga cattttgaag
      121 gaaatcaagg acgccggcgc gaagaacatc gccgtgggaa ctcagctcat tcaggacaag
      181 gcggggtcgt gaggaacttt tcgaccgcgc cggtgtcctt caaatcgggg accgtgtccc
      241 cggaggcctt ttaatgcgcc attgggagat gctttacgat cgtcagcccc tggggctgga
      301 cgcggccctt ttcggggtcg cccttctcct ccacgcgccc ctgctcctga ttcaattcaa
      361 agcggccccc cactcgcacc gggcggaacc ccgggtggtc aaggtggaat tgcttgaaaa
      421 tttaaatacc caggttttct cgaaccccat ttccgtccgg ccgaccacgg gccccgtcat
      481 caaaagcccc atcgacatgc cccagtacaa ggatttgatt aaacgcatcc aggaggccag
      541 actgaagggc gaccttcttc acgtggtcaa accccacgtc gtaccgccgg ctattcccaa
      601 ggccggcttg ggcacgggtc ccatcaaatt gccggatgcg gggctgaaca acaagggcgc
      661 tttcaatcac gtggcggccc cccaattggg aagcggtccg gcggggctta aaggcgggtc
      721 ggcggcggcc gggcccgcgg ccaccgggcg ccccctgggc gacatcggcg gcggtccggc
      781 gggacccgcg ctcctcggca acaagggcag tttttccatt cgatcgggcc aaatcggctc
      841 catcggatcc ggcgagggca tcaaagttgg ggccgggggc gccggggcca tcgcgattaa
      901 aaccacgaaa accgggtcgg tggacgacgc ggtgttggat cccattccgg tccaggacaa
      961 agggcgtttc ggcgctttgg ggggcggcga cggatcgggg ggaaccctgg ccggc
//
LOCUS       DAOQLS010000898         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_147462_length_1014_cov_4.113660, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000898 DAOQLS010000000
VERSION     DAOQLS010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147462_length_1014_cov_4.113660"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PK362_12380"
     CDS             complement(<1..687)
                     /locus_tag="PK362_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75669.1"
                     /translation="MKMTAQFIISMVLAVGLVAAGSAYLLMRQERSNQTDELNRRSAL
                     VAEGLEESVSTAMAQGSTKDLQRILRRFENKSRLTGIVVFDVKDKALAGTTVLLKSLP
                     EKSDLVAEVIDTRQARGEYVKWRGKSDYAYAYPIVLNDQPAGALLIVHDATYIHGYLA
                     RIWRHTFGRALVQMAVITLLILGLMRWSVFGPIHQMAEWMKKLRAGDTVETPPLPHSE
                     LFAPLAKEASR"
     gene            complement(684..>1014)
                     /locus_tag="PK362_12385"
     CDS             complement(684..>1014)
                     /locus_tag="PK362_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75670.1"
                     /translation="ATVAAAQFIGTGLLSALLDNNVVADFAGRALRGFELTVVHASAM
                     AQIAGYAVGGCLTHIGSAQSVVAFAFIRKDIAPHFTPWDWFKTMGPFVLKLTALLMAV
                     ILLEVLL"
BASE COUNT          211 a          282 c          322 g          199 t
ORIGIN      
        1 ccgggaggcc tctttggcca ggggggcgaa aagttcggaa tggggcaggg gcggagtttc
       61 gacggtatcg cccgcgcgca atttcttcat ccattcggcc atttggtgga tggggccgaa
      121 gacgctccat cgcatcaacc cgaggatgag cagggtaatc accgccattt gaaccagggc
      181 tcggccgaag gtgtggcgcc agatgcgggc caagtacccg tgaatgtagg tcgcgtcgtg
      241 aacgatcaaa agggcgccgg cgggctgatc gttgaggacg atcggatagg cgtaggcgta
      301 atcggacttg ccgcgccatt taacgtattc gccccgggct tggcgggtgt cgatgacttc
      361 ggccaccagg tcggatttct cgggaaggga ttttaaaagg accgtggtcc ccgccaacgc
      421 cttgtccttc acgtcaaaaa caacgatgcc cgttaaacgg gatttgtttt caaaccgccg
      481 aagaatcctt tggaggtcct tcgtggatcc ctgggccatt gcggtgctca ccgattcctc
      541 gaggccctcg gccaccaggg cggaccggcg gttcaattca tcggtttgat tgcttcgctc
      601 ctgtcgcatg agcaagtagg ccgatcccgc ggccaccagg cccaccgcca acaccatgga
      661 aataataaat tgcgccgtca ttttcatagg aggacctcga ggaggattac ggccatcagg
      721 agggccgtta atttcaggac aaaaggcccc atggttttga accaatccca gggggtgaaa
      781 tgcggggcga tgtcttttcg gatgaaggca aaagccacca ccgattgggc ggatccgatg
      841 tgggtcaaac atccgcccac ggcgtagccg gcaatttggg ccatggccga ggcgtggaca
      901 acggtgagtt cgaatccccg cagggcccgt ccggcgaaat cggccaccac gttgttgtcc
      961 aaaagggcgg acaggagtcc cgttccgatg aattgggcgg cggccaccgt cgcc
//
LOCUS       DAOQLS010000899         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_147866_length_1013_cov_2.501044, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000899 DAOQLS010000000
VERSION     DAOQLS010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147866_length_1013_cov_2.501044"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PK362_12390"
     CDS             <1..912
                     /locus_tag="PK362_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNC75671.1"
                     /translation="GKLKVESARETIRDINPNVNVKTYNLPFTRDVAMDIVSHYDVVV
                     DGTDNFQTRYLTNDTCVFLKKPNVYASIFRFDGQATVFNPSDAESPCYRCLYPEPPPP
                     GEVPSCAEGGVLGILPGLVGLIQATETIKLIIGKGRSLVGRLLLFNALEMSFDELKIR
                     RNPNCPVCGKNPTITQLVDYDQFCGVGRGNEKEADMNGLAEMTVKELKARMDRKDAFV
                     LVDVREPSEYEIAQIPGARLIPLGDVAVRANELDTADDIIVHCRSGVRSAKAIQVLKQ
                     LGFKKLTNVKGGILAWSDEIDPSVPKY"
BASE COUNT          225 a          279 c          306 g          203 t
ORIGIN      
        1 gggaagctca aggtcgaaag cgcccgggaa accattcggg acatcaaccc caacgtcaac
       61 gtgaaaacct ataacctgcc gttcacccgg gatgtggcca tggacatcgt cagccattac
      121 gacgtggttg tggacggtac ggataatttt caaacccggt acctcaccaa cgacacctgc
      181 gtttttctca agaaaccgaa cgtgtacgcg tcgatctttc ggttcgacgg ccaggccacg
      241 gtgttcaatc ccagcgacgc cgagagcccc tgctaccgct gtctctaccc ggaaccgccg
      301 ccgccgggcg aagttccgtc ctgcgccgag gggggcgtgc tggggatcct cccggggctg
      361 gtggggttga ttcaggcgac ggagaccatc aagttgatca tcgggaaagg ccggtctttg
      421 gtggggcggt tgctgttgtt caacgcgctg gaaatgagct tcgacgaatt gaaaatccgg
      481 cgcaacccga actgtcctgt gtgtggaaaa aatccaacga tcacccaatt ggtggattac
      541 gatcaattct gcggggtggg acgcggcaat gaaaaggagg ccgacatgaa cggcttggcg
      601 gaaatgacag tgaaagagtt gaaggcccgc atggaccgaa aggacgcgtt tgttctcgtg
      661 gatgttcggg aacccagcga atacgaaatc gcccaaatcc ccggggcgcg cctcattccc
      721 ctgggggatg tggccgtccg cgccaacgag ctggacaccg ccgacgacat tattgtccat
      781 tgccgatccg gtgtccgcag tgcgaaggcc attcaagtgc tgaaacagtt gggcttcaaa
      841 aaactgacca acgtcaaggg cggcatcttg gcgtggtccg acgaaatcga tccgtccgtt
      901 ccaaaatatt gattcgatca gtttccatgg gcgccggatc gcgttacggg gtgggtctgc
      961 ttgaccgcat tgggaacacg ccgcttttaa aacttgagcg gctgtcccgc tgg
//
LOCUS       DAOQLS010000900         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_148033_length_1012_cov_4.538140, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000900 DAOQLS010000000
VERSION     DAOQLS010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148033_length_1012_cov_4.538140"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK362_12395"
     CDS             complement(<1..282)
                     /locus_tag="PK362_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75672.1"
                     /translation="MSPLPPDNTSASLSPAPTPRAPAAPVRWLPPFLRRSVRPLGRHI
                     ASLISLRTRLLWVLLLAFVPAFTLIVRDHFEWEQQDHREISDEAMLNARL"
     gene            complement(385..>1012)
                     /locus_tag="PK362_12400"
     CDS             complement(385..>1012)
                     /locus_tag="PK362_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75673.1"
                     /translation="PRPRCWPSSSRVVCRPDRRPRLRGNDWSGLRIATPGPETTALLA
                     LRLARPGFTPVDMPFDKIIAAVQSGRVDAGLIIHEGQLTYRTHGLEKVLDLGQWWHRE
                     TRLPLPLGLDVVRKDLGIRLARELAAALTQSIRLAYRDKKASVAYALRYGRGIGAAVG
                     EKFVKMYVNRDTLDLGPQGEKALRLLYDRARKAGWLPRRPTFRVVRPR"
BASE COUNT          182 a          299 c          373 g          158 t
ORIGIN      
        1 cagacgggcg ttgagcatgg cttcgtcgga tatttcccgg tggtcctgct gttcccattc
       61 aaaatggtcc cgaacaatca gcgtgaaggc cgggacaaaa gcgagcagga ggacccagag
      121 caaccgggtg cgcaggctga tgagggaggc gatgtggcgc cccagggggc ggaccgaccg
      181 ccgaaggaaa ggcggcaacc agcggacggg ggcggccggg gcccgcggag tcggggccgg
      241 cgaaagggag gcgctcgtgt tgtcgggggg caaaggggac atgcggtcgt tcggtcgttg
      301 ggaaccggca cctcggtctg cgtccggaat cgaaaacgtc ttcccttata tagcccccga
      361 acggggcggt gtcaagacgg ccggttaacg ggggcgaacg acgcggaagg tgggtcgccg
      421 gggcagccaa ccggcttttc gggcccggtc gtaaagcagg cggagggcct tttcgccctg
      481 gggacccaga tcgagggtgt ctcggttgac gtacattttc acgaattttt cccccaccgc
      541 cgccccgatg ccccggccgt agcggagagc gtaggccacg gaagcttttt tgtcccggta
      601 ggcgagtcga atgctttgcg tcaaggccgc ggccaattcc cgggcgaggc ggattccgag
      661 gtccttgcgg accacgtcga gccccagggg caacggcaaa cgggtttccc ggtgccacca
      721 ttggcccaag tccaggacct tttccaaccc gtgggtgcgg taggtcagct ggccttcgtg
      781 aatgatcaaa cccgcgtcca cgcgacccga ttgaacggcg gcgatgattt tatcaaaggg
      841 catgtccacg ggggtaaacc cgggacgggc caggcgaagc gccaggaggg cggtggtttc
      901 ggggcccggc gtcgcgatgc ggagcccgga ccaatcgttg ccgcgaagcc gcggacggcg
      961 gtccggccgg cagaccacgc gtgagctgct cggccagcac ctcggcctgg ga
//
LOCUS       DAOQLS010000901         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_148408_length_1011_cov_3.328452, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000901 DAOQLS010000000
VERSION     DAOQLS010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148408_length_1011_cov_3.328452"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PK362_12405"
     CDS             <1..892
                     /locus_tag="PK362_12405"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HNC75674.1"
                     /translation="LFESWVQAPCDVEIASEFRYRNPLIGPNDLLVAISQSGETADTI
                     AAVRHAKEKGATTLAICNSVGSTLYRECHGKILTHCGPEIGVASTKAFTAQLAVLYVL
                     ALYGALLRGTLPQPDVEKIMKNLLHLPRWVGEALKTDSVVAALAKKYFHSRNFLFLGR
                     NLNYPIALEGALKLKEISYIHAEGYPSGELKHGPIALIDEDMPVVAIATQGRVYDKVM
                     TNLEEVSARGGRVIALATAGDTRITALTSDIVWMPDVPESMAPAVNVVPLQLLAYHIA
                     VLRGCDVDQPRNLAKSVTVE"
BASE COUNT          209 a          319 c          286 g          197 t
ORIGIN      
        1 cttgttcgag tcctgggttc aagcgccctg cgatgtggag atcgcctccg aattccgcta
       61 tcggaacccc ctcatcggcc cgaacgattt gttggtggcg atttcccagt cgggcgaaac
      121 ggccgacacc atcgccgcgg ttcgccacgc caaagaaaag ggtgcgacca cgttggcgat
      181 ctgcaattcg gtggggtcga ccctttaccg cgaatgccac gggaaaattc tcacccactg
      241 cggtccggaa atcggcgtgg ccagcacgaa ggccttcacc gcccaactgg cggttctcta
      301 tgtgttggcc ctttacggcg ccctgttgcg gggcacgttg ccccaacccg acgtggagaa
      361 aatcatgaag aacttgctcc acctgccaag gtgggtgggc gaagctctta aaacggattc
      421 ggtggtggcc gccctggcga aaaaatattt ccacagtcgg aatttcctgt tcctcgggcg
      481 aaatctcaac tatcccatcg ccctggaagg ggcgttgaag ctgaaagaaa tttcttacat
      541 ccacgcggaa ggctatccct ccggcgaact gaaacacggc cccatcgcct tgatcgacga
      601 agacatgccg gtggtggcca tcgccaccca aggccgcgtc tacgacaagg tcatgaccaa
      661 cttggaagaa gtgtccgccc ggggcgggcg tgtgatcgcc ctggccaccg cgggcgacac
      721 gcgcatcacc gccttgacct cggacatcgt ttggatgccg gacgtccccg aatccatggc
      781 cccggcggtg aacgtggtgc cccttcaact gttggcctac cacatcgccg tcctccgcgg
      841 ctgtgacgtg gaccaacccc ggaaccttgc caaatccgtc acagttgagt gaagaatccg
      901 cccatcaggc tgaaatcgtg aaacggagca ctgcccgaaa atctctcatt cctcgtggct
      961 atgcgagctg gttgagtggc ctaaagtcgc gcatctccgg tgcgaaacag c
//
LOCUS       DAOQLS010000902         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_149084_length_1009_cov_2.146751, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000902 DAOQLS010000000
VERSION     DAOQLS010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149084_length_1009_cov_2.146751"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..701
                     /locus_tag="PK362_12410"
     CDS             108..701
                     /locus_tag="PK362_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75675.1"
                     /translation="MDEYRKAIQESKAAARRYAARRAVELGVSPEAYLREKAKKRKER
                     RLDRKKKALRRERKFAKALREKRIPHVVTGWAVQVSAAYKDLPALRRREGWGGNKGDV
                     PPAEQAAYDRLSTQGHKALRAARVGTADGYEYFLQGITFYFFVRRDPRRAQKIITTVF
                     QRGQPFRRSKGTLVTFYKDRTSDRVPFVLRGKKVPND"
     gene            694..>1009
                     /locus_tag="PK362_12415"
     CDS             694..>1009
                     /locus_tag="PK362_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75676.1"
                     /translation="MIKKPSETKSKARVWLEQNANPFPLATNRFGTKKNALSYVKKLE
                     ALGAVDVKVADVLDEPWRIKKEKGPYATSLKVTLPDAKAPRKRLFAFIAREKKHPRAE
                     VPE"
BASE COUNT          256 a          288 c          288 g          177 t
ORIGIN      
        1 caggccatgg aactacgggg ggtcaaattt ccggccacgc ccccggccca ggtgcgaata
       61 gaaaaaaatt taaatgccaa tgactgaatt tccagcggag ttccaaaatg gatgaatatc
      121 gaaaagcgat tcaagaatcc aaagcggcgg ctcgtcgtta cgccgcccgc cgcgcggtgg
      181 agttgggggt ttctcccgaa gcctatcttc gcgaaaaggc gaaaaagcgc aaagagcgtc
      241 gattggaccg aaagaaaaag gctctgcgtc gagaaagaaa attcgccaaa gccttgcgag
      301 aaaagcgcat tccgcacgtt gtgaccggat gggccgtcca ggtcagcgcg gcttataagg
      361 atttgcccgc cctacgccgg cgggaggggt gggggggaaa caagggggac gttcccccgg
      421 ccgaacaagc cgcctacgat cgtttgtcca cccaagggca caaggccctt cgggcggccc
      481 gggtgggaac ggcggacggt tacgaatatt ttcttcaggg catcacgttt tatttctttg
      541 tccgccggga tccccggcgc gcgcaaaaaa taatcaccac ggtgttccag cgtgggcagc
      601 cctttcgccg gtccaaggga accctcgtca ctttttataa agaccgaacc tccgaccggg
      661 ttccctttgt ccttcgagga aagaaggttc ccaatgatta aaaagccgtc cgaaaccaaa
      721 tccaaagccc gggtatggct ggagcaaaac gccaacccct tccccttggc caccaatcgg
      781 tttgggacaa agaagaacgc cctgtcctac gtaaaaaagt tggaagccct gggcgcggtg
      841 gacgtaaagg tggcggatgt tttggacgaa ccttggcgaa tcaaaaaaga aaagggccct
      901 tacgccacgt cgctcaaggt caccctgccg gacgccaaag ccccccgcaa gcggctcttt
      961 gccttcatcg cccgggaaaa aaaacacccc cgcgccgaag tgccggagg
//
LOCUS       DAOQLS010000903         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_149280_length_1008_cov_2.971668, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000903 DAOQLS010000000
VERSION     DAOQLS010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149280_length_1008_cov_2.971668"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1008)
                     /locus_tag="PK362_12420"
     CDS             complement(<1..>1008)
                     /locus_tag="PK362_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNC75677.1"
                     /translation="VLAAVVVALVERFVPGLEIATIGRRFHFQIDGRAWDGVPGILPA
                     LRAPWSFPGPGGAPLAFNVALLEALAPSALAIALLAAIESLLSAVVADGMAGTRHNPD
                     AELTALGVGNLVGPFFGGIPATGAIARTATNIRSGARSPLAAAFHGLFILGVLLAAAP
                     LLGRVPMASLAALLLVVAYHMSELRHVRYLLNVAPRSDAAVLLICFGLTVVFDMVAGV
                     SVGIVLASLLFMRRMAATTRGRTLGSETGAGASPPPGFVYYEIAGPMFFGAVDNAIDA
                     IASVDRSVRGVLFLMDKIPVMDVSGLVALESAIRKLSAAGQLSVLVGAHGQPQALIRK
                     S"
BASE COUNT          220 a          358 c          321 g          109 t
ORIGIN      
        1 gcgatttgcg aatcaacgcc tggggttggc cgtgggcccc caccagaacg gacaactggc
       61 ccgccgcgga caacttccga atggcgcttt cgagcgccac caaccccgaa acgtccatga
      121 ccgggatttt gtccatcaaa aacagcaccc cgcgaacgct ccggtccacc gaggcaatgg
      181 catcgatggc gttgtccacc gcgccgaaaa acatgggccc ggcgatctcg taataaacga
      241 accccggggg cggcgaagcc ccggcgcccg tttcgcttcc cagggtccgg ccccgggtgg
      301 tggccgccat ccgacgcata aacagcaggg acgccaaaac gatgccgacg gaaacgcccg
      361 ccaccatgtc aaaaacgacc gtcaagccga aacagatcaa caacaccgcc gcatcgctcc
      421 ggggcgcgac gtttaaaaga taacgcacgt gccggagttc cgacatgtgg taggcgacga
      481 ccagcagcaa cgccgccaga gaggccatgg gcacccgccc cagcaggggc gccgcggcga
      541 gcagaacccc cagaataaaa agaccgtgga acgccgccgc caggggcgac cgggccccgc
      601 ttcggatgtt ggtggccgtc cgggcgatgg cgcccgtggc cgggatgccg ccgaagaaag
      661 gccccaccaa gttgcccacg cccagggccg tgagctccgc gtcggggttg tgccgggtgc
      721 cggccatgcc gtcggccacc accgccgaca agagggattc gatggccgcc aaaagcgcga
      781 tggccaaagc cgagggcgcc agggcctcca ggagcgcgac attgaacgcg aggggcgcgc
      841 ccccgggccc ggggaacgac cagggggccc gcaacgcggg cagtatcccc gggacgccgt
      901 cccacgcccg gccgtcgatt tgaaaatgaa aacgccggcc gatggtggcg atttccaacc
      961 ccgggacaaa ccgttccacg agcgccacga ccaccgcggc caaaacca
//
LOCUS       DAOQLS010000904         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_150047_length_1006_cov_1.837014, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000904 DAOQLS010000000
VERSION     DAOQLS010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150047_length_1006_cov_1.837014"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK362_12425"
     CDS             <1..532
                     /locus_tag="PK362_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75678.1"
                     /translation="WAMAAIFLAAFSRVCVGDGTVITPVDPDSQTEDKVIFENRRLSL
                     GLTYVGGGIRWGLQKGWSSEVRYVTGQEGSAGGTVKAQFLSARAAKHWMSGHRWAPYG
                     AIEGGTLRAADSSRKASGTAFGLVGGIEFAMTRKLSITFEMGPYSIGLKDKVGGIGGQ
                     GTDIVLGSTLYWHLFQ"
     gene            543..>1006
                     /locus_tag="PK362_12430"
     CDS             543..>1006
                     /locus_tag="PK362_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75679.1"
                     /translation="MVKRPTTRGILLGGMPFLAGCVLWAGVHFGVGGPAAGRGKEIFE
                     QEQGRLWAKITTPAAGALVAGRTPVSLEVGPGINRVEFETEEGIAATVVDPPFGWVWD
                     TRSGPDGARRVTVRAYGAGGAPVTSDVSVTVDNTPPRVSLSPLSPPLTAASTV"
BASE COUNT          182 a          267 c          352 g          205 t
ORIGIN      
        1 ctgggccatg gcggccatct ttctggccgc tttttcacgg gtctgtgtcg gtgatggaac
       61 ggtgatcacc cccgttgacc ccgattccca aacagaagac aaagtcattt ttgaaaacag
      121 gcgattgtcc ctcggattaa cctacgtcgg gggcggaata cgctgggggt tacaaaaagg
      181 atggtcttcg gaagttcgat atgtgaccgg ccaggaaggt tcggcgggtg ggactgtgaa
      241 ggcccaattt cttagcgccc gggcggccaa acattggatg tccggtcatc gctgggcgcc
      301 ttacggcgcc attgaaggcg ggacattgcg cgccgcagac tcttcccgaa aagcgtctgg
      361 gacggcgttc gggctggtgg gaggaattga gttcgccatg acaagaaagt tatccatcac
      421 attcgaaatg ggcccttact ccattgggct gaaagataag gtcggaggca tcggaggtca
      481 gggcaccgac atcgttttgg ggagcacttt atattggcac ctatttcaat gaggagagga
      541 ccatggtgaa acggccgacg actcgaggca ttttgttggg ggggatgcca tttctcgcgg
      601 gttgcgttct atgggcgggg gtgcattttg gcgtcggcgg cccggcggcc ggccggggaa
      661 aagagatctt cgagcaggaa cagggccgcc tgtgggcgaa aatcacgacc ccggcggcgg
      721 gcgccttagt ggccgggcga acaccggttt ctttggaggt cggcccgggc ataaatcggg
      781 tggagtttga gacggaggag gggatcgccg cgacggtggt cgatcctccg ttcggctggg
      841 tctgggatac ccggtccggc ccggacgggg cccgtcgagt gacggttcgg gcgtacggcg
      901 cgggtggcgc gccggtcacc tccgacgtct ccgtgacggt ggacaacacg cctccccgtg
      961 tgagcctgtc gccgctttca cctccgctga cggcggcctc caccgt
//
LOCUS       DAOQLS010000905         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_150350_length_1005_cov_1.938947, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000905 DAOQLS010000000
VERSION     DAOQLS010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150350_length_1005_cov_1.938947"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PK362_12435"
     CDS             <1..464
                     /locus_tag="PK362_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC75680.1"
                     /translation="PVKLEKQLAFMASRDAAVSFTSFRRINRDGSRVGDPVAIPPRLN
                     YRQLLSNTALATSTVIVDRARTGTFRMTPTYYDDFALWLDLLKRGVDAHGLSEDLMRY
                     RVVGKSVSRNKFRSALWVWRTYRGVERLSWIDAAVCFAQYALRALWKYRRF"
     gene            472..951
                     /locus_tag="PK362_12440"
     CDS             472..951
                     /locus_tag="PK362_12440"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC75681.1"
                     /translation="MSRGRVLVTGANGFVGRALMSALRDGGLDFRAAVRSADRRPPAA
                     EFVAVGDLGSRTDWRNALAGVDRVVHLAGRAHVMSPGDDDAFQRVNAEGTAGLARAAA
                     AAGVRRFILLSTVGVHGARSPGDRPLTEESPIAPVGPYARSKWEGEESLRAIGVLFF"
BASE COUNT          138 a          307 c          359 g          201 t
ORIGIN      
        1 cccccgtcaa attggaaaag caactcgcgt tcatggcgtc ccgggacgcg gcggtgagct
       61 ttacgtcctt tcgccgcatc aaccgggacg gcagccgggt cggcgacccg gtggccattc
      121 cgccgcggct caattaccgc cagttgttgt cgaacaccgc gctggccacg tcgaccgtca
      181 tcgtggaccg ggcgcgaacg gggacgttcc ggatgacccc gacgtattac gacgatttcg
      241 ccttgtggct cgatttgtta aaacggggcg tcgacgccca cggcctttcc gaggacctca
      301 tgcgctaccg ggtggtcggg aaatccgtgt cccgcaataa attccgttcg gcgctgtggg
      361 tgtggcgcac gtatcggggc gtcgaacgcc tttcgtggat cgacgcggcc gtttgctttg
      421 cccaatacgc cctgcgcgcg ctttggaaat atcgacgatt ttgaggccgg cgtgagccgc
      481 ggccgggtct tggtgacggg cgccaacggt tttgtgggtc gggccctgat gtcggcgctt
      541 cgggacggcg gtctcgattt tcgagcggcg gtccgttccg cggatcgtcg tcccccggcg
      601 gccgaatttg tcgccgtggg cgacctgggc tcccggacgg attggcggaa cgctttggcc
      661 ggggtggatc gggtggtcca tttggccggc cgggcccacg tgatgtcgcc cggggacgac
      721 gacgcttttc aacgggtgaa cgcggagggc accgccggtc tggcccgggc cgcggcggcg
      781 gcgggcgttc ggcgttttat tcttttaagc acggtggggg ttcacggggc gcgctcgccc
      841 ggggatcggc ccttgaccga ggaaagtccc atcgcgcccg tgggccccta cgcccgatcc
      901 aaatgggagg gggaagaatc ccttcgggcg atcggcgttt tattctttta agcacggtgg
      961 gggttcacgg ggcgcgctcg cccaaggatc ggcccttgac cgagg
//
LOCUS       DAOQLS010000906         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_150450_length_1004_cov_4.751317, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000906 DAOQLS010000000
VERSION     DAOQLS010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150450_length_1004_cov_4.751317"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PK362_12445"
     CDS             complement(<1..541)
                     /locus_tag="PK362_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNC75682.1"
                     /translation="MNILDMIDKPQDLRNIRKDLLPILAKDIRQKILDVTSQVGGHLG
                     AGLGTVELAIALHYVFESPKDKIIWDTGHQAYPHKLLTGRRDRFHTLRQFGGISGFLS
                     RNESEHDVFGAGHASTSISAALGFAAARDVLKQDHHVIAVANDGSLTGGMAFEALQNA
                     GHLGTDLLVVLNDNQMFISH"
     gene            complement(543..>1004)
                     /locus_tag="PK362_12450"
     CDS             complement(543..>1004)
                     /locus_tag="PK362_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC75683.1"
                     /translation="RAVGSQGMVGGQAVDLESEGRGVALNGSRAKVLDYIHRHKTGAL
                     ITASLSVGALLSGASPKAQRALERYGDQIGLAFQIADDVLDIVGNKALLGKSGSDAEN
                     QKLTYPAVYGLEESQRRARRAVDEAKRLLKPFGRRADLLVALADYIITREN"
BASE COUNT          164 a          308 c          341 g          191 t
ORIGIN      
        1 ggtgggagat gaacatttgg ttgtcgttca gcaccaccaa aagatccgtg ccgagatgcc
       61 cggcgttttg cagggcttcg aaggccatgc cgccggtcag ggagccgtcg ttggccacgg
      121 cgatcacgtg gtggtcctgc ttcaacacgt cccgggcggc ggcgaagccc agggcggccg
      181 aaatggaggt cgaggcgtgg cccgcgccga acacgtcgtg ctccgattcg ttccgggaaa
      241 ggaagcccga aatgccgccg aactgccgca gggtgtggaa gcggtcccgg cgacccgtga
      301 gcagtttgtg gggataggcc tgatggcccg tgtcccagat gattttgtcc ttgggcgact
      361 cgaagacgta atggagggcg atggccagct ccacggtgcc caaaccggcg cccaggtggc
      421 cgcccacctg ggaggtcacg tccaggattt tctggcggat gtccttggcg agaatgggca
      481 ggaggtcctt gcggatgttc cgcaggtcct ggggtttgtc gatcatgtcc aaaatattca
      541 tgtcagttct ctcgggtgat gatgtaatcg gccagggcca ccaacagatc ggcccggcgg
      601 ccgaagggtt tcaaaaggcg tttggcttcg tccacggccc gtcgggcccg gcgctgggat
      661 tcctccaggc cgtagaccgc ggggtacgtc agcttttggt tctcggcgtc ggagccgctt
      721 ttgcccagga gcgccttgtt gcccacgatg tccaacacgt cgtcggcgat ttgaaacgcc
      781 aaaccgattt gatcgccgta gcgctccagg gcccgttggg ccttgggcga ggcgccggag
      841 agcagcgccc ccaccgacag gctcgcggtg atgagggcgc cggttttgtg gcggtggatg
      901 tagtccaaca ccttggcccg gcttccgttc agggccacgc cgcgcccttc ggattccaaa
      961 tccaccgcct ggccccccac catgccctgg gaccccacgg cccg
//
LOCUS       DAOQLS010000907         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Elusimicrobiota bacterium isolate AMR_MDS_0542
            NODE_151442_length_1001_cov_3.548626, whole genome shotgun
            sequence.
ACCESSION   DAOQLS010000907 DAOQLS010000000
VERSION     DAOQLS010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810174
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Elusimicrobiota bacterium (wastewater metagenome)
  ORGANISM  Elusimicrobiota bacterium
            Bacteria; Elusimicrobiota.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,454
            Genes (coding)                    :: 2,430
            CDSs (with protein)               :: 2,430
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 1 of 24
            Pseudo Genes (incomplete)         :: 23 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Elusimicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151442_length_1001_cov_3.548626"
                     /isolate="AMR_MDS_0542"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2030800"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK362_12455"
     CDS             <1..629
                     /locus_tag="PK362_12455"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNC75684.1"
                     /translation="WARDADSAAVLSAAGGTSAGVGSVWGRGLQGFARPTSLKRVLWI
                     PRAPASAEEWAGKVNGLRALEPWDHGFLMMHPAQDRALLGALRARLTFPHRLETWEPP
                     DDVLAVLGRYDLVVSMRYHGLVAAALTSRPAAALAAHGKVDVLARELGAPRLSWGATA
                     TDWRERIRAAFDRGPASPGERPAVARRDLAALERWLSDAGVPLPSQNL"
     gene            665..>1001
                     /locus_tag="PK362_12460"
     CDS             665..>1001
                     /locus_tag="PK362_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC75685.1"
                     /translation="MSAPALSAAPRRVAAVGRVAWPGGWPLPVTLGVLSAALGFSVSL
                     LTQEQRMSSAFAAAESDAFRWVVFLKNSADRPSLEERLKSLPGLRRLEFTSKEQALES
                     AQRDPALAEG"
BASE COUNT          115 a          341 c          362 g          183 t
ORIGIN      
        1 tgtgggcccg ggacgcggat tccgcggcgg tcctttccgc cgccggcgga acgtcggcgg
       61 gcgtgggttc cgtctggggc cgcggccttc aggggtttgc ccggccgacg tcgttgaagc
      121 gcgtcctctg gattccccgg gcgccggcgt ccgcggagga atgggccggg aaagtgaacg
      181 gacttcgcgc cctcgaaccc tgggaccacg gattcttgat gatgcacccg gcccaggacc
      241 gagcgctcct gggggccttg cgggcccgct tgacgtttcc gcaccggttg gaaacctggg
      301 aacctcccga cgatgtgttg gcggttctgg ggcgttacga cctggtggtg tccatgcggt
      361 atcacggcct ggtggccgcc gccctgacgt cccggccggc ggcggccttg gcggcccacg
      421 ggaaggtgga cgttttggcc cgggaattgg gcgcgccccg cctgtcctgg ggcgcgaccg
      481 cgacggattg gcgggagcgg atccgcgcgg ccttcgaccg ggggcccgcg tcgccggggg
      541 aacgacccgc cgtcgcccga cgggatttgg cggccctgga gcggtggctc tccgacgcgg
      601 gagtcccgct tccctctcaa aatttgtaaa atgccggcgt gaaacttttc cttcggttcg
      661 gccggtgagc gcccccgctt tgtcggcggc cccgcggcgc gtcgccgcgg tcggacgcgt
      721 cgcctggccc ggagggtggc ccctgcccgt cacgctcggc gtcctgtccg ccgcgctcgg
      781 cttttccgtt tcccttctga cccaggaaca gcggatgtcg tcggccttcg ccgcggcgga
      841 atccgacgcc tttcgttggg tcgtgttttt gaaaaacagc gccgatcgtc cctccctgga
      901 agaacggttg aaatcccttc cggggcttcg acggctcgag ttcacctcca aggaacaggc
      961 cttggagtcc gcccagcggg atccggccct ggccgaaggg c
//
